LOCUS       DAOQVY010000001       108970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_198_length_108970_cov_6.167791, whole genome shotgun sequence.
ACCESSION   DAOQVY010000001 DAOQVY010000000
VERSION     DAOQVY010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 108970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 108970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..108970
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198_length_108970_cov_6.167791"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..447
                     /locus_tag="PKN91_00005"
     CDS             175..447
                     /locus_tag="PKN91_00005"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNS26479.1"
                     /translation="MSAPEPRIEYGVQLGAFSSEDKARAEWQRISGKFANVLGGLSPA
                     VSPVKSGAGSLYRLRASTASESAARGICESLRAQGQPCVVVLPVGK"
     gene            874..2202
                     /locus_tag="PKN91_00010"
     CDS             874..2202
                     /locus_tag="PKN91_00010"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNS26480.1"
                     /translation="MHRLILRGALIALLLVTTAHAAAPAEIRTTTLANGMRVIVWPDH
                     DIPNVALYNYVRVGSRNEAPGITGLAHFFEHMMFNGTTRRPPGEFDREMEARGGRNNA
                     STSDDLTIYMDWFPAGSIEAVLDLEGDRLANLAFDPQVIESERGVVYSERRLRVEDDN
                     NGFLFEQVQSTAFAAHPYHLPTIGWPSDIEGWTLADLQAFYRTYYAPNNCTLVVVGDV
                     LPDAVFALARQYLEPIPRQEPPPAVRTLEPVQPGEKHVAVERRAQTPLLQLAYKAPQA
                     DDPRNPALNLLSVILGRGDASRLHRLLVEDRKVAISVDTYLQEGFDPGLFWVFATLPA
                     GGDPAGVQAQIDAEFARLIEDGVTDAELSRARNLLSAAYWRQLATIDGKAALLGEFEV
                     MHGDYRKLFDLPDAFARVTAEDIRAVAKELLDPRRRTVGVLTPPANAGEG"
     gene            2204..3628
                     /locus_tag="PKN91_00015"
     CDS             2204..3628
                     /locus_tag="PKN91_00015"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNS26481.1"
                     /translation="MRTIAIALCAAVALLATAAPAPAAGVVVPPYTRTVLPNGATLLV
                     MPREDVPLIAFTAIVRGGSVAETGEQAGVASLTAGLLEKGAGSRNAFEFADTVEGAGG
                     SFSAAAASEAIVVSGQFLAQDRELMVELLADALLRPHFAAAEFDSLRNRRIESIRAAK
                     DSDPSGLIRSYGRAFLFAKHPYGRAASGSERSLASITRDDVLAYYRANFGGDRLTLIV
                     AGDVDTVALTKSVEAAFAGWARAAVPLPALASPVRTTGRRVLLVDQPGAAQTYFWLAN
                     TGVARNFAARAPLEVVNTLYGGRFTSILNTELRIKSGLSYGASSGFVRGSVPGEFAIS
                     SFTQTDTTAAAIDLALATLAGLHEQGLTDDMLASSRTYVLGQFPLQLETASHWVAALA
                     ELELYGLDRSYIEGYGGAVEGVDRQAAQRVIEEAFPRPENLAIVMIGDAAKIRAVAAR
                     YGPVTEMPLSAPDFAAPPAPRPVR"
     gene            complement(3632..4498)
                     /locus_tag="PKN91_00020"
     CDS             complement(3632..4498)
                     /locus_tag="PKN91_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNS26482.1"
                     /translation="MPIERFIDARERNLGGFNVRRILPAPGRRMVGPFIFFDHIGPAD
                     FPPGRGIDVRPHPHIALATVTYLYAGAFLHRDSLGNVMEIRPGDVNWMTAGRGIAHSE
                     RTPPRLRERGAHMHGIQSWVALPDGHEEIEPAFAHHPAATLPHLEVDGCELRVIAGEA
                     YGRRSPVEVLWPTLYVDASMPAGALLEMPADYTERAIYVSEGEVDIDGEAVGAARLAL
                     LARDATVTLRAVHNSRVMLLGGEPFATPRHIWWNFVASSRERIEQAKQDWARGRFAPV
                     VDETEFTPLPDD"
     gene            4531..8655
                     /locus_tag="PKN91_00025"
     CDS             4531..8655
                     /locus_tag="PKN91_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26483.1"
                     /translation="MDDGEGQSGSDAVRLFPRVVGLVLLSFVSATQVQAADPHVPAAL
                     QDWRGWVLTGEEHRACARLASPGVAAGSREARSCVWPGRLVLDVGARGGEFRQAWQVE
                     ADSWVALPGNADQWPRDVTIDGRRGAIVARNGIPHARLAPGEYQIAGRFEWTARPESL
                     AVPAETGLVDLVVDGTRVAAPTRTGNTLLLGARQGVQQSVSFDFDVHRLLVDDIPLRL
                     VTRLTLRATGGSREVLLGPVLPAGFAPIALDSPLPARVEADGRLRVQLRAGVFGIDLI
                     ARAVDGAAQVTRPSAAEPWPAEEIWSFAGDDRLRVAALEGGESIDPAQADVPAEWRAY
                     PAMRVLQDTALGVVERSRGMASLDDNQLQVHRQLWLDFDHAGFTAVDQLRGSMRSGWR
                     LDMAAPWSLGSARLDDVPLLVTRGQGEGTTGVELRSPELVLQTVARSAGARGALPATG
                     WTTRFEAVNGELHVPPGHRLVAALGVDSAPDAWLERWGLWSLFGVVIIAAFVGWLAGK
                     PAGVLAFAALALTYQDAPQVIWLWGNLLAALALARAAPEGRLRGFALRYRTISFAVLA
                     IVLVPFMWSQLRLALHPQLDSAYSAGWNLPMPMPAARAPAAPPPPVMDMATARPEMAA
                     VQEVKQETPEPRARKDVIATGAMASRTERYAPGTVVQAGPGIPTWQYRSYAYSWSGPV
                     EPSQTARFVIAGPFVLAAWRVVGVLLLAALFAVLLAGSGPNWRERLPPRWRPWLTHAA
                     ARGTAPLAVALCFGVALVAGPSPAAAQLPDSQLLGELKARLTKPAECEPSCAELLAAR
                     VEVDPARLTLTIDAAALAHLAVPVPAAPGAWLLDSVTVDGTPSHALRRGGDEHLWVPL
                     QPGTRTIVVSGRIASTDAVQLVFPWPPKRVVVAASGWEVAGVSDGRLPGGSLDLSRHT
                     EAGARPVAGVDVAAEFPPFARVVRRFEIGAETRVETRVERVAPLRAAFSLPVPLLVGE
                     SVLTEDVEVRDGQTALAVLPAGAAAVGWASTLPRADSLTLTAPAADSRLVEVWEFDVD
                     AQWHATFEGFVATLPEELGGEWVFEFHPRAGEQLTAKFERPVAAEGAPLAIEAVERTL
                     SIGRRATDSTLAVRYRATQGGRHTVKLAPEARITSVTVDGEALALRPENGELSIPILP
                     GEHTVTVSSTHASGVATIVKPEPLDLGAPASNVRTTLSLPANRWALFAAGDGVGPAIL
                     YWSELVAFLLTAWLVGRSGRSPLRTHEWVLLGLGLSTLSWGVLALVAIWLFAMKWREG
                     WAGQVVRWQFNTVQVLLAVLTYWAVTALLFYGIRDGLLSTPDMGVTGPGSGGNRYSWF
                     LDRTGGALPRPMVLSLPLWVFKSLVFVWAVWAAFAVLRWLRAAWNAWKSGGYWR"
     gene            complement(8752..9132)
                     /locus_tag="PKN91_00030"
     CDS             complement(8752..9132)
                     /locus_tag="PKN91_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26484.1"
                     /translation="MSKRKASAASQPGPRLHKVSQYLERNFPDFFAETRFQVGDDDYF
                     LYARFGQYLARSIEQRRVPREKIERGFTVLNKMARISARDPGVRRMLISGPLEYIVDA
                     PKARALARKRLSPIAQGYLASLCE"
     gene            complement(9191..10789)
                     /locus_tag="PKN91_00035"
     CDS             complement(9191..10789)
                     /locus_tag="PKN91_00035"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNS26485.1"
                     /translation="MGFKHPGSALLLAGVLVACGGGGDGSIGGGGGGGGGGGGGGGGT
                     GFTPGFFPASSTYESRCANPRSGNDPVSGDPWPDRLGTALDEKNWLRSWTHELYLWYR
                     EVPDLNPAGYGVLEYFDLLKTDALTPSGRPKDQFHFTYLTSEWQQLSESGVSAGYGLQ
                     WALLSATPPRQLVVAYIEPDGTSPATMAGLARGAQVLAIDGIDLVNTNVDSEIDALNE
                     AIYPSDTGLSHSFTVQDIGGGPQRTVSMTSTVVTAAPVQNVRTIATPNGLVGYLLFND
                     HIATAELALVDAITTLRNQGIVDLVLDVRYNGGGYLDIASELAYMIAGGAQTQGRTFE
                     LLQFNDQHPTRDPVTGRTIAPMPFHTVAQDFSVAGGTPLPALDLPRVFVLTGPGTCSA
                     SEAIINSLRGVDVMVVQIGSTTCGKPYGFYPWDNCGTTYFSIQFRGVNAKNFGDYADG
                     FSPENTVGTVGVPLPGCSVADDFNHALGDPAEARLSTALHFRQTGLCGVPPSGMAQAQ
                     VGALPLKDGVTRKSPWLENRILRR"
     gene            11025..15494
                     /gene="gltB"
                     /locus_tag="PKN91_00040"
     CDS             11025..15494
                     /gene="gltB"
                     /locus_tag="PKN91_00040"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNS26486.1"
                     /translation="MTASGRGLYRDSYERDSCGFGLIASLDDQPSHWLIETAILSLAR
                     LTHRGAIAADGKTGDGCGLLLKKPEKFLRTVATEAGLKLSRRFAAGNVFLDPRPEKAG
                     AARAELEAQLTREGLEVAGWRVLPTDSRACGEEALKTLPRIEQLFVNCRIPDTDEASF
                     NRRLFLARRRAEKALEASDPVFYVPSLSASTLVYKGMVMPQHLTEFYPDLSDTRLESS
                     VAVFHQRFSTNTLPQWRLAHPFRLLAHNGEINTIQGNRNWAVARGPLFRSPLLPSLDD
                     IQPLVSLTGSDSQSLDNMLEVFLMGGLDVLHAMRMLVPPAWQTLDSIDSDLRAFYEYY
                     ATHMEPWDGPAGIVTTDGRYAACLLDRNGLRPARYVITKNRHLTVASETGVWDYRPAD
                     IVAKGKLGPGDMIALDLHSGKLLSTRDIDQILKTRHPYKTWLKRGVRYLETDLIDPRL
                     AAEPMDRDTLGLYQKMFNITAEERDEIIRVLAADESEAVGSMGDDTPMPVLSHQVRSL
                     YDYFRQQFAQVTNPPIDPLREAIVMSLQTQIGPECNIFAPSPEHARQIVLGSPILSQR
                     KLRQILGLTDFGVTHQFIDLQYPQEESLEAAIERICDEAERAVRDDTLVLLLSDRYLV
                     RGKLPIHALLATGAVHHRLVRTGLRSKCNLLVETGTARDPHHFACLIGYGATAVYPFM
                     AYQTLFDMMRKGRLKLDAEERLEIGRRYRAGIRKGLYKVMSKMGITTIASYRSSQLFE
                     IVGLADKVVDLCFRGTESRVQGADFDDLARDAAQLAARAWNPREPIAPGGLHKYVHGG
                     EYHMYNPDVIATLQAAVMTGEYARYREFARLVNERPVSAFRDLLRLKGGAAPIAIDAV
                     EPIDTIIKRFDSAGMSLGALSPEAHEALAIAMNRLGARSNSGEGGEDPARYGSEKNSK
                     IKQIASGRFGVTPAYLVSAEVLQIKVAQGAKPGEGGQLPGHKVNEMIAHLRFARPGVG
                     LISPPPHHDIYSIEDLAQLIFDLKQVNPAALVSVKLVAEPGVGTVAAGVAKAYADLIT
                     VSGHDGGTGASPISSIHYAGTPWELGLAETHQTLRQMDLRHKVRLQTDGGLKTGLDVV
                     KAAIIGAESFGFGTAPMVALGCKYLRICHLNNCATGVATQHPVLRSKHFIGLPEMVMN
                     YFRFVAQEVREILAGLGVARLADIIGRTELLEILEGETARQRKLDLGPILSTGGLVAD
                     RPQYCIDPSNRPFDKGELAERMVADMRGAIEARSGGEWHYSVSNFNRSIGARVSGEIA
                     RRWGNHGMAEHPLTVRLTGNVGQSFGVWNAGGLHLYLEGDANDYVGKGMAGGRIVLRH
                     PGTAGFVHRDTIIMGNTCLYGATGGELFAAGVAGERFAVRNSGAVAVVEGTGDHCCEY
                     MTGGVVCVLGRTGVNFGAGFTGGFAYVLDLERDFVDRYNHELIDISRLQVEAMQAHVQ
                     HLRALIGRHVEETGSVWGEEMLNDFRSYVGKFWVVKPKAASIDSLIENLRRAA"
     gene            15491..16900
                     /locus_tag="PKN91_00045"
     CDS             15491..16900
                     /locus_tag="PKN91_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS26487.1"
                     /translation="MSDKHLQFLEIPRRDPEKLPVEERVAKSAEIYRQYDAEAASQQA
                     GRCLSCGNPFCEWKCPVHNYIPNWLKLIEEGNLFEAAELSHRTNSLPEVCGRICPQDR
                     LCEGACTLNDGLGAVSIGAIEKYITDEALKQGWKPDMSNVTPTGRRVGIVGAGPAGLA
                     CADILARSGVQPIVYDRYRRIGGLLTYGIPPFKLDKEVVEKRREILEGMGVEFRLGVQ
                     IGRDIAFDAFLADHDAVFLGMGTYTHVKGGFPGEELPGVYEALPYLISSIRHELGESD
                     AEALIDLRGRRVVVLGGGDTSMDCNRTAIRQGATSVKCTYRRDEANMPGSRRDYKNSI
                     EEGVEFLFNRQPIEIVGSDRVEGVKLVETRLGPPDARGRRLPETVPGSEEIIPADAVV
                     IAFGFQPSPAPWFAAHDIALHPNGRVRVSAGRTTPFQTTNPKVFAGGDMVRGADLVVT
                     AVFEGREAAKGILGYLGIA"
     gene            complement(17018..18895)
                     /locus_tag="PKN91_00050"
     CDS             complement(17018..18895)
                     /locus_tag="PKN91_00050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNS26488.1"
                     /translation="MPATDMLARVRGAKAPVAVGKAGGRPMLSWVGKRAPATVQAFPA
                     QLVETYGGAAPDDTLWDTWPDGLRRGGLLYHGDNKEVLAHLLANGFRGQIKLIYIDPP
                     FDSGADYVRKVELRGPKGKVKLEGEGYTLGEQIQYTDIWANDNYLQFMYERLLLLKEL
                     MAQESIIVLHCDWKKSHHLRCVMDEVFGSQSFQNEVYWYFYNKMHDDRKGILPRATNT
                     LLVYGLGSAQKLHKLASPRENVVKQLKRVKVDGVLENLKDEQGNLVYQDSDSKTLDNV
                     WGIPLIPPADHKQKTDYPTQKPEGLLDLVLHGYTNPGELVLDAFIGSGTTAVSAQRLG
                     RRWIGCDINKGAIQTTAKRLQRVVEEQLTAGGKPAQGKLLDESGSHAPAPAQLGFTHW
                     RVNDYDLAIQHNEAVNLACEYLGVERLRSDAFFDGLLGKRLVKIIPFGHPLSPMDLEA
                     IRNELEARPDEDRAITVVCLGRELAADRWLADWNRLRKKKTDPHHIEVIELRSDPKYG
                     GFMQHEKAQARVAVKRKGGKLHVEIKDVVSPGILERLAQQSGVVQAKVADWRAMVDSV
                     MIDTGYDGAVFNVVHADVPIRRTDFVVGEYKLDAPDGTTTVAVKVTDMLGEEVLVVCK
                     A"
     gene            complement(18985..21618)
                     /locus_tag="PKN91_00055"
     CDS             complement(18985..21618)
                     /locus_tag="PKN91_00055"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNS26489.1"
                     /translation="MQLPRLIAEKLAGWRAAGYPHERYAAIAEILDWLSGRDAGQPPF
                     LRIPQVRALETYWYMRLVEDTPGIFDLYQKLAPTRVELRKTLGLTSDVVREYAEEHGF
                     DALIEHVLSDDAFVKQHKLESLRETLTLHYPSYILALAMGAGKTMLIGAIIATEFAMA
                     QEHPDEEFVHNALVFAPGKTIIESLRELATMPFDGILPPRMHKLFSASVKLTFTRDGS
                     PDIDVLPGSHYNIVVTNTEKIRIQKSAIRKGDLGSLFAGAGDADQLRQDVANRRLQRL
                     AQLPHLGVFSDEAHHTYGQSMDAELKKVRKTVDYLAAETNVVCVVNTTGTPYYNKQPL
                     LDVVYWYGLSQGIEDGYLKPVSGNIEAYDFGDDLKPYVAEVVADFLEHYGDVALPNGA
                     RAKLALYFPQTDDLEAMRPVVEAALAANGRAPTECLANHSRSPQADVDAFNRLNDPMA
                     PHRVILLVNRGTEGWNCPSLFACALARNLQSSNNFVLQAATRCLRQVPGNAHKAKIYL
                     SQDNYTVLDRQLQETYGESLAELNRNQANLRHAKIVLLKENIPPLVLKRQIVRVRRVE
                     TRIPAKLELQRPQDSDPALTVQRYELAAQVSSTRVMRELGDALPVDATIDAADTRTVA
                     ADLAARYRLPLFAVLDALRSTYRDDDVPHRHLDALADQLEAQVRHYETESEEIEVALA
                     LVRQEGFDEEQDADGKPVRTAEITYRKDKADRLWSVEDARADYGGNFGFHYSPYNFDS
                     LPEKDFLAKVLDTLRLDTGDIEDVYFTGALGRDKTDFVVEYKGEDGRWHPYSPDFLIR
                     RKPQAGGAPGSGKLLIVEIKQERLRADYTDGESGRKAMAVREWARLNPDRIAYEMLFT
                     DTSTLANNDFAPVRTFLGAKA"
     gene            complement(21637..22041)
                     /locus_tag="PKN91_00060"
     CDS             complement(21637..22041)
                     /locus_tag="PKN91_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNS26490.1"
                     /translation="MTRVLVDANVLLDVLTDDPQWHGWSAAQLDACAAEAELCINPIV
                     YAEISVGFERIEELDDALPSDAFTRLELPWEAGFLAGKAFLQYRRTRGARTSPLPDFY
                     IGAHAAIEGMALLTRDAKRYRTYYPKLELICP"
     gene            complement(22052..22300)
                     /locus_tag="PKN91_00065"
     CDS             complement(22052..22300)
                     /locus_tag="PKN91_00065"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HNS26491.1"
                     /translation="MTATADRLDLRLSVKDKNRLRRAAELHGLPVATFVREAALREAE
                     TTIAHPPKARRGSLAARLRGRATARMGTNEIMRLTRGA"
     gene            complement(22297..22539)
                     /locus_tag="PKN91_00070"
     CDS             complement(22297..22539)
                     /locus_tag="PKN91_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNS26492.1"
                     /translation="MRRKSRWDTDAEAGACGSRESSMAKAAVPEALFRIADRCVQVVG
                     GIGVSGDTIVEPVRCGFLPSGGQTACTVCYRTRRLI"
     gene            complement(22589..23578)
                     /locus_tag="PKN91_00075"
     CDS             complement(22589..23578)
                     /locus_tag="PKN91_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNS26493.1"
                     /translation="MKPQRRILVAIKDPASSTPPAVAKAARLAQALGAELELFHAMTI
                     PISADTHLYETGSLANFERGARKRAVEQLEAMAGPLRRKKLTVRTAADWDYPAHEAIV
                     RRARRIKAELIVADLHAGRRLAPWLLHLTDWELLRTSPFPVLLVKNDEAWGRPIVLAA
                     IDPTHAFAKPAGLDSEILKVAARLSSALGGSLHAMHAYGPIPVGVHPEVGVGPREVAN
                     IKDAAESAARKALDRALRGTKIPARRRHLVVGSPVKAIPRTAGELGSAIVVMGAVSRS
                     ALKRVVIGNTAERVLRALPCDVLVVKPAKFVTGVSRTPRGVRYMMSTKLPLLY"
     gene            23787..24143
                     /locus_tag="PKN91_00080"
     CDS             23787..24143
                     /locus_tag="PKN91_00080"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNS26494.1"
                     /translation="MARTAPKTRICLWYDREAEEAANFYAGLFPDSRVLSVQRAPADY
                     PSGKAGDVLVVEFTVLGVECGWCKDKWGVSWQITPLALTEAMASADPAVAKRVFEAMM
                     TMRKIDIAAIEKARRG"
     gene            complement(24173..25330)
                     /locus_tag="PKN91_00085"
     CDS             complement(24173..25330)
                     /locus_tag="PKN91_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNS26495.1"
                     /translation="MTEQIPLARRVEAFVREKIAPYERDLRRGPHGPAKDLVTELRDL
                     ARAAGLLTPHILADGSHLSHRETAQVLRAAGLSPLGPVALNVAAPDEGNMYLLGRVAS
                     PEQQRRFLQPLIEGRTRSAFLMTEPAEDEGAGSDPSMMKTTAVLDGDHWVVNGRKAFI
                     TGADDAGVGIVMAKTANGAIMLLIELPHPAVTIERVLDTIDSSMPGGHAIVRIDNLRV
                     HCDQMLGQSGEGFKYAQVRLAPARLTHCMRWLGACTRAQEIASEYAVKRTAFGKPLID
                     HEGVGFMLAENRIELKQAELMIDWCAGVLDSGAAGTSESSMAKVAVSEALFRVADRCV
                     QVMGGTGVSGDTIVEQVFREVRAFRIYDGPTEVHKWSLAKRIKRETLQRLA"
     gene            complement(25359..26759)
                     /locus_tag="PKN91_00090"
     CDS             complement(25359..26759)
                     /locus_tag="PKN91_00090"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS26496.1"
                     /translation="MEKPHEAALMRLQSRAYAGTVLGVLLLVYTSNFIDRTILGTLGQ
                     AVKDDLGISDTQLGLLQGFAFAVLYTLLGIPLARLAERRSRVGIISICLFVWSGFTAL
                     CGMAQNFAQLLLYRIGVGIGEAGCSPAAHSLITDYFPAQRRATALSIYSFGVPLGTMI
                     GAVAGGYIAQTFGWRQAFLIVGLPGIVLAVVTWIVVREPQRGQCDPIESRPLDTAPPA
                     PLGAVVRRLAGCRSFRHMACGATLISFAAYGGSTFLAPYFLRSFALDYAQVGLIYGLI
                     AGLGAGAGTLLGGRICDRIGEREPKWYALFPGLTLLLATPLYLLAWVQDTWTVAAGLL
                     LVAAFLHYMYLGPTFGVMHNLVDARMRATATALLLFVINFVGLGGGPLFTGWLIDLFS
                     AQAFAPFGFGEFLTSCPGGRAPQDSGPELLAACRESLERGTRGGQFITHAIIAWAAVH
                     FLLGARWLRGDLARAG"
     gene            complement(27259..27993)
                     /locus_tag="PKN91_00095"
     CDS             complement(27259..27993)
                     /locus_tag="PKN91_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26497.1"
                     /translation="MTKSILQRDHRSVMGRILYTSRKPERLGQERGREYFRIDTHASG
                     ARTITAHCEIDDRPSVMRDITYALDAEWMPTDCFVRLSVGDRFAGSGWFRFAPDLTEC
                     ETWTAAEGRVRQTMALARPLVTFQNHAIACDAWHLRLIDRKRPGEVQRIPQMLLSSPD
                     HRGATGPMLHSIGVNIVYVGPEKVTVGAGTFDAWRYQFVTNVELPEEHPPYDVWCTSD
                     GDFLFLQGGVAGYMQTWYELVELERS"
     gene            complement(27990..29327)
                     /locus_tag="PKN91_00100"
     CDS             complement(27990..29327)
                     /locus_tag="PKN91_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNS26498.1"
                     /translation="MHWRDLAGDHRREIFAIAGPMVLANLSAPLIGLVDTAVVGHLGE
                     ASYIGGVAVGAVILSFAFTVLNFLRMTTTGLAAQAAGAGNATRLRTVLAQGVGSALYL
                     GALLLIAQVPLGMLALRLIEPSAAVDAQAWTYYSIRIWSAPAALANFALIGWFVGIGN
                     ARAPLVMVLSANLVNIALDLLFVPGLGYGVAGVAWASLIAEYLAAALGLRLVVRELGA
                     RPGHWRRADILDLRSLRQLFAANGALFLRTLLLMFAFAFLTSMGARQTDAILAANAIL
                     VNLQYLMAYLLDGYANAVESLTGRAIGARDGTSLRSIVDECLAWTLAIALAIALAYGA
                     AGPLAIRVVTSVPGVIEAAYTYLPWMVLSPLVCAWAFLYDGVFIGAQRPGAMFGVMLV
                     AVGVVFLPAWWLTRGFGNHGLWFAFMLFNAARGLQQHLLFRSWMRRGTILEVR"
     gene            complement(29331..30893)
                     /locus_tag="PKN91_00105"
     CDS             complement(29331..30893)
                     /locus_tag="PKN91_00105"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNS26499.1"
                     /translation="MYKSLVIALLCAALAACGVNPVTGKKEIQFVSEAQEVSIGQQQY
                     APMRQVEGGDLEVLPELTAYVSGVGQKLAAVSDRHLPYEFVVLNNSVPNAWALPGGKI
                     AVNRGLLTELTSEAELAAVLAHEIVHAAARHGAKAQERGTLMQAGMAAAQIGAAVGGM
                     DAGLANLALGGASIGLQMVTMKYGRDAELESDFYGMKYMKAAGYDPWGAVTLQETFVR
                     LAGDKARNKSWLDGLFASHPPSQERVDRNRATATELGRGGVIGDDTYAARVRPLLDMK
                     PGFDKYDEALAAARKNELDRANSLALEAARLLPQDGRTQQLLGDIAVARKNPQAALGY
                     YRKAEALAPNYFGSHLGTGIASYQLGDKATAASAFERSAKLLPTAPAAYYLGAIARDR
                     GDRATALQYFKAAAASNSSYGKEATAELVRLDLPQNPGNYVAAAVQADASGRVYVVIE
                     NRTPLPIAAIVVTPVLVDANGNILQRGAAVQIRGPVAAGQRASAQINTGNLTAEQMRY
                     LRARVDSARLAQ"
     gene            complement(30916..32103)
                     /locus_tag="PKN91_00110"
     CDS             complement(30916..32103)
                     /locus_tag="PKN91_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNS26500.1"
                     /translation="MAAPASKKHVALDPMDLYGVRALLTEDEQMVQDSVARLVDDQVL
                     PVIRECFEQHRFPKELVPELAALGLLGSSIDGYGCAGLNSVSYGLICQELERGDSGIR
                     SFVSVQSSLCMYPIHAFGSEEQKQKYLPRMAKGELIGCFGLTEPHGGSDPSNMKTHAK
                     KRGKDWVINGAKMWITNGAIADLCIAWAMTDEGIRGFIVEKGTKGFTAPEIENKFSLR
                     ASVTSALFFDNVVVPEEQMLPGVVGLRGPLSCLTQARYGITWGVIGAAQACLAQMIDY
                     TKSRVLFGRPLAQTQAIQIRLADMARRITTAQLLSLQLGRLKDKGDMVPSQVSLAKWN
                     NCRMALDVARDARDMLGGAGISAEYVPIRHMLNLESVITYEGTETVHQLTIGRELTGI
                     NAF"
     gene            complement(32168..32665)
                     /locus_tag="PKN91_00115"
     CDS             complement(32168..32665)
                     /locus_tag="PKN91_00115"
                     /inference="COORDINATES: protein motif:HMM:NF017897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNS26501.1"
                     /translation="MRTVTQWLDEYGESHRNPVNKLLHWICVPAIVVSVIGLLWSLPV
                     PAAFRAVSPWLNWATITALPGLAYYFRLSPALALGNVLVFVAVFAIVHGLLRLPWPLW
                     QTCAGIFVVAWIGQFIGHACEGRRPSFFKDLQFLLIGPLWLLAFVYRALRLSYGPRPR
                     AGQNT"
     gene            32715..33254
                     /locus_tag="PKN91_00120"
     CDS             32715..33254
                     /locus_tag="PKN91_00120"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNS26502.1"
                     /translation="MSASSARPRQRLLGWLATREWLSDARKLEWYPPFWLMRIRVLEL
                     APDWRVVRIRLPLSSISRNPGGVMFGGFQASLADPIPALACARIFPGYSVWTRGLAID
                     FDRGGSTDLEFRFEMPLSQEADIRAELAARGRATPTFDCGYYLKDGTRCTRVRNTVAI
                     RPQGYDRATTPPASPDSVG"
     gene            33429..33581
                     /locus_tag="PKN91_00125"
                     /pseudo
     CDS             33429..33581
                     /locus_tag="PKN91_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024833.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
     gene            33666..34007
                     /locus_tag="PKN91_00130"
     CDS             33666..34007
                     /locus_tag="PKN91_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNS26503.1"
                     /translation="MPGNITFQTDSADLNASFFGVLDSVVLVAKEYDKTIIEAAGHTD
                     STGSEQYNQQLSERRAGTVAAYLTNRGILADRVITVGAGEMRPIATNDTPEGRTQNRR
                     VELTFAPIRAQ"
     gene            complement(34066..34932)
                     /gene="rpoH"
                     /locus_tag="PKN91_00135"
     CDS             complement(34066..34932)
                     /gene="rpoH"
                     /locus_tag="PKN91_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HNS26504.1"
                     /translation="MQLVARRNDFLLSGPIGSFDAYLDQISRIPVLTREREQELATRF
                     HEEGDLAAARDLVLSHLRFVVHVARGYGGYGLPLADIVQEGNVGLMKAVKRFDPAMGV
                     RLVSFAVHWIRAEIHEYVLRNWRLVKVATTKAQRKLFFNLRRMKKNLAWLTHEETQAV
                     ARDLGVRPEEVTEMEQRLSSRDLSFDPVPDLDDDDEGHGPAAYLPAPDADPAELVAEA
                     EAEADTSQHLAHAIEALDARSRDIVQRRWVDDRKATLHDLADEYGVSAERIRQIESAA
                     LKKLRGALTAAA"
     gene            complement(35074..36000)
                     /gene="ftsX"
                     /locus_tag="PKN91_00140"
     CDS             complement(35074..36000)
                     /gene="ftsX"
                     /locus_tag="PKN91_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNS26505.1"
                     /translation="MIGPVTWSAHHIQAALGAAGRLARAPLSTLFTVGVIGLALALPL
                     GLKLFVDNARAATGDFANAVDLSVYFAIGTPLEKVQQLAERARARSGVATATVIPADA
                     ALAEFRQYSGFGAALDAIEDNPLPHVLGVRPQAGADSPADLAKLREYLTSWPEVELVQ
                     MDVEWVNRFNAILEVLRRALLVAALVLAAGVLAVVGNTIRLEIQNRRAEIEVTKLVGG
                     SNAFVRRPFLYTGVLYGLLGALLAAAIVLAATSVLAPAVADLAAAYGSRYQMLGASRL
                     DTLRLVGGGAALGWLGAWLAAARQLRLIEPRA"
     gene            complement(35997..36692)
                     /gene="ftsE"
                     /locus_tag="PKN91_00145"
     CDS             complement(35997..36692)
                     /gene="ftsE"
                     /locus_tag="PKN91_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNS26506.1"
                     /translation="MIRFERVAKRYPNGREAIAGVSFEVARGEMVFLTGRSGAGKSTV
                     LKLIALLERPTRGVVTVNGQSTGPLPARRIPAFRRQIGVVFQDHKLLVDRPVFDNVAL
                     PLVVAGTPLRELDKRVRAALDQVGLLGKEQLLPLELSIGEQQRVGIARAVISRPAVLI
                     ADEPTGNLDPELALEVMNVFRRFQEVGTTVMVATHDMHLVREFGKREIVLEGGHVRGV
                     EEVLVPTVGAAAP"
     gene            complement(36689..37654)
                     /gene="ftsY"
                     /locus_tag="PKN91_00150"
     CDS             complement(36689..37654)
                     /gene="ftsY"
                     /locus_tag="PKN91_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNS26507.1"
                     /translation="MATIESDDGNAREGFFKRLRAKLNKPNSWLSYDLANLFRGREID
                     EVALEELEARLLTADVGVEATEQILEALAQRAARKELDDVEALVDALKNAIFEILEPC
                     AKPLDISGSRKPFVVLVVGVNGSGKTTTIGKLAQRLQHEGKSVLLAAGDTFRAAAIEQ
                     LGVWADRTGAAMVAQQAGADPAAVVFDALQSARARGTQVLICDTAGRLQSQSHLMDEL
                     KKVKRVIQRADPSAPHEVLLVIDANQGQNALAQAREFNEAVGVTGLALTKLDGTAKGG
                     IAVAIARQLRLPIRFIGIGESAEDFGVFDARAFATALVEGREAAE"
     gene            complement(37709..39760)
                     /locus_tag="PKN91_00155"
     CDS             complement(37709..39760)
                     /locus_tag="PKN91_00155"
                     /inference="COORDINATES: protein motif:HMM:NF023683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26508.1"
                     /translation="MGPKQGSLTLAATTSILLLLAGCSGGSGIDGPDIGPTDTTSGSL
                     PGGSTVTPAPTGLRPLFQVAQGLLPWPTDLYFAGSTDGTLNISPANSLMPAQAALNAL
                     DGYGVNTVIRARFSGPVNAASLTPASVRVIRVNIDNATKATVGIAGVLVPGVDYSVGL
                     AADAAVGNTIVEIRPLMPLVPSTGATNNGYLVLLTDAITDAAGAAAVPDHDYATIKTA
                     LAGGATCPSITNPTLNGVCQLTGAHLQIAGAVGVNPASVVLSFSFSTGSTRDSLAAVA
                     ATATAQPIVAQATGLDTSQINPALPGVADIYAGILQIPYFLSKSAPLTGSWQGNPSPL
                     GGTTHLTRFNPLPVATETLSIPLLATAPNAASGRTQPAAGWPVVIFQHGITGDRTNML
                     GLADAFAGQGFVVVAIDQPLHGITDTTSPLYQAPNERTFNLDVMNNATGAAGPDGVID
                     SSGASFINLTSLLTARDNLRQAAADLITLTRSVGDLDLTGDGVSDIDATRIHFVGLSL
                     GSIVAVPWISLSAGVQTATLSVPGGLIAQLLQDSPTFGPRINAGLQAQGLIPGTTLYS
                     QFFRDAQTVIEAGDPVNYMAAAAAAKPILLQQVLGDTVVPNSATQRLIDLGGLAKVGT
                     PGLNPASRAYVNMTAGTHASLLDPTASLAATIEMQTQAVTFAVSDGTLVAISDPTVVE
                     P"
     gene            39820..40413
                     /gene="rsmD"
                     /locus_tag="PKN91_00160"
     CDS             39820..40413
                     /gene="rsmD"
                     /locus_tag="PKN91_00160"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNS26509.1"
                     /translation="MASNSRRSARQLRIIGGAWRGRKFRFPPVDSIRPTPDRVRETLF
                     NWLMGRVDGARALDLFAGSGALGLEALSRGAAEAIFVERDARVARHLEGVLAELDARH
                     ARVVNADALRFLRGPARPFDLVFLDPPFDSELAGLAAGALEAGGWLAPAALIYLELPV
                     AVPVPPLPGTWRIVRSGRAGEVGYHLAERAARGAGAA"
     gene            40410..40898
                     /gene="coaD"
                     /locus_tag="PKN91_00165"
     CDS             40410..40898
                     /gene="coaD"
                     /locus_tag="PKN91_00165"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004751128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNS26510.1"
                     /translation="MRRHAVYPGTFDPITNGHHDLVRRAATIFSRVVVAISENPGKQP
                     LLSLDRRLALAREVLVDLPQVEVMSYKGLTVEFARQQSLTVIVRGLRAVSDFEFEFQL
                     ANMSRHLSDDIETVFLTPKEQFTFISSTLVREIAVLGGDVSQFVHPIVEAELRKHRRE
                     PS"
     gene            40895..42571
                     /gene="ggt"
                     /locus_tag="PKN91_00170"
     CDS             40895..42571
                     /gene="ggt"
                     /locus_tag="PKN91_00170"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNS26511.1"
                     /translation="MRRAVVAFSLLLATLFATALPAAGPAPGKAAIASAHPLATAAGH
                     EILAKGGNAFDAAVAVSAALAVVEPIGSGLGGGGFYLLHRASDKLDVMVDAREKAPAA
                     ASRDMFLDADGNVVRALARDSALSAGISGEPAGMELLAQKYGRLPLAVSLAPAIRFAN
                     EGFPLYPRLQANIQRKRDVFATQPEVAAVWLRDGESPALGTIIRQPDLARTLERLARH
                     GVRDFYEGALARELVAGVRRLGGNWTAADLAAYRALERAPIVGAYRGARIVSASPPAS
                     GGIVLVEALNILSGYDLAQVDAATRKHLIIESMRRAHRDRALWLGDPDFASMPVAELI
                     HPYYAAGLRASLRLDRATPSAELPGVEPPPQGTQTTHFSILDAEGNRVAGTITLNAFF
                     GAGLVVPGTGVFLNNQMDDFSSKPGVPNGFGLVGASANAIAPGKRMLSSSTPTFVESD
                     RGLMIAGSPGGSFITGMVLLATLDFLDGKDAVEIVGAPRIHHQYLPDVLQFETDALTA
                     VERATLAQRGHNLREANRRWGNMQVVTWDYASGRVEAASDPRGHGEGQVY"
     gene            42549..42953
                     /locus_tag="PKN91_00175"
     CDS             42549..42953
                     /locus_tag="PKN91_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03067 domain-containing protein"
                     /protein_id="HNS26512.1"
                     /translation="MAKDRSTEVLERRLDGSWLPTAAFVAAEEVPVGELRVARLDLAA
                     GRYRIFDREEQILDSGEYSIDAAVLPVAMDMVGVAGPNAGRRLEAIIDIEGDTLTVCY
                     DLDGLARPESMRPEKDQLLLRISYARCLQRPS"
     gene            complement(42841..43650)
                     /locus_tag="PKN91_00180"
     CDS             complement(42841..43650)
                     /locus_tag="PKN91_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HNS26513.1"
                     /translation="MRKHFAARDPVMAALIRAAGPYRLAPEGARSPFESLARAITHQQ
                     LNGIAAERILGRFVALCGSGAFPEPGDVLARPDHELRAVGLSFAKIASLKDLAAKTGN
                     GIVPHGAALATLSDAQIIDRLTQVRGIGRWTVEMLLIFQLGRPDVLPVDDFGVRNGFR
                     LAYGLRGMPTPRALAAFGERWAPHRTAAAWYLWRAVDIAKLGKLPAPVAKPRGIRIVK
                     RKSAAGNGAPRKPAVRKGAAGSARKKSAAATGLSRAASTRAARGRRGRSRR"
     gene            complement(43647..44462)
                     /gene="mutM"
                     /locus_tag="PKN91_00185"
     CDS             complement(43647..44462)
                     /gene="mutM"
                     /locus_tag="PKN91_00185"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNS26514.1"
                     /translation="MPELPEVETTRRGIEPHVLDRRIVTLAVHEPRLRWPVDAQLPAR
                     VAGQRVLRTRRRAKYLLLDLESGTLLLHLGMSGNLRVLPAATPRIAHDHFDLVLDSGA
                     TLRFNDPRRFGSLLYTEDDPARHPLLAALAPEPLEAQFDAAYLWRVTRRRRVAIKSLI
                     MNARLVVGVGNIYASEALFRARIRPGRQARHLTAREVARLVRAIRTVLAGAVRAGGTT
                     LRDYVGADGAPGYFRQKLYVYERTGQPCRVCRTPIRRRVQGQRATYWCPECQR"
     gene            complement(44541..44696)
                     /gene="rpmG"
                     /locus_tag="PKN91_00190"
     CDS             complement(44541..44696)
                     /gene="rpmG"
                     /locus_tag="PKN91_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNS26515.1"
                     /translation="MREKIKLLSSAGTGHFYVTMKNKRLHPEKMETKKYDPVVRKHVS
                     YKEAKIK"
     gene            complement(44710..44946)
                     /gene="rpmB"
                     /locus_tag="PKN91_00195"
     CDS             complement(44710..44946)
                     /gene="rpmB"
                     /locus_tag="PKN91_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018677334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNS26516.1"
                     /translation="MSRVCEITGKRPAVGNRVSHANNKKRRRFLPNLHVQRFWLEDEK
                     RWISLRVSSNGLRTIEKNGIDAVVADLRTKGVKV"
     gene            complement(45052..45441)
                     /locus_tag="PKN91_00200"
     CDS             complement(45052..45441)
                     /locus_tag="PKN91_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26517.1"
                     /translation="MSARPPLGTRAPLTARTRPCPHCKAVILESASVCPGCRHHLRFD
                     PVAAQRQGETRTAMRIDGTIRGPDPAEPCEYCVTVAIRDAHGKEIGRHVIGVGAMRPG
                     EERSFTLAVEVLPPVGPRSQPRPPGRK"
     gene            complement(45438..45956)
                     /locus_tag="PKN91_00205"
     CDS             complement(45438..45956)
                     /locus_tag="PKN91_00205"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNS26518.1"
                     /translation="MALWKDTTTPGSSPAPIPSAAKDPVREVERPAVAPLNSAHDTRR
                     PKERVELKETVISSGLNIEGKIEGAGNVRISGKFKGDVHIEGDLTIDAGAQLNGQVRA
                     NLVAIAGELQGNVESARRVELAETAVMTGDVKAGALTVAAGSRMRGQVEFGWDEKHSR
                     GHGEARGTASGT"
     gene            complement(46045..46719)
                     /gene="radC"
                     /locus_tag="PKN91_00210"
     CDS             complement(46045..46719)
                     /gene="radC"
                     /locus_tag="PKN91_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNS26519.1"
                     /translation="MGIRAWPAGEQPREKLVNLGVRQLSDAELLAVLIGSGSAGLDAV
                     ALARELLLCTGSLRGLLGSDRETVCRLRGLGLARWCRLQAALEVARRHYQETLQARPV
                     LDAPRHTAEFLVAQLRDRPYEVFCCLHLDSRHRLIALDELFRGTIDGASVHPREVVRQ
                     ALARNAAAVILAHNHPSGVAEASQADELITRRLRDALALMDIRVLDHVIVGDGACMSF
                     AERGLL"
     gene            46826..48061
                     /gene="coaBC"
                     /locus_tag="PKN91_00215"
     CDS             46826..48061
                     /gene="coaBC"
                     /locus_tag="PKN91_00215"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNS26520.1"
                     /translation="MQGRKILLGVTGGIAAYKSPDLVRRLAERGAQVQVVMTAGAAQF
                     VTATTFQAVSGRAVRDDLWDPAAEAAMGHIELARWADLVLIAPATAHSLAGLAQGHAD
                     DLLGTLCLATEAPIAVAPAMNRVMWANPATQANVAVLRARGVHVWGPGSGDQACGETG
                     DGRMLEPLELAGLAEALLGAPRGPLAGRRVLVTAGPTRERIDPVRFISNRSSGKMGFA
                     IARAARDLGAEVLLVTGPVALPTPAGVRRIDIESAEDMLAAVTREVATTDIYISTAAI
                     ADYRPVRPAAQKIKKKEAALDLSLERTVDVLAAVAARADRPFVVGFAAETESVEQNAR
                     EKLLRKKLDLIAANEVGDDKVFDKDDNALVVISRTDRTELGPAAKAQLADALVALIAE
                     RYAAHRAAQDAATHGKARA"
     gene            48058..48534
                     /gene="dut"
                     /locus_tag="PKN91_00220"
     CDS             48058..48534
                     /gene="dut"
                     /locus_tag="PKN91_00220"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNS26521.1"
                     /translation="MSGVAATAHRPLKVRILDPRIGGEWPLPAYATPGAAGLDLRAAL
                     DAPLELAPGRSELIPTGLAIWIEDPALAAVILPRSGLGHKHGVVLGNLVGLIDSDYQG
                     PLMVSCWNRGAAAYTVQPGERIAQLVVVPVVQVQFDVVEDFAATARGAGGFGHTGR"
     gene            complement(48558..49217)
                     /locus_tag="PKN91_00225"
     CDS             complement(48558..49217)
                     /locus_tag="PKN91_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNS26522.1"
                     /translation="MLRWPALTVTLTLAVLAAGPVDAATKNKNKGADTGEVSYRWTDE
                     KGVVHYGDHVPPEYAKSERAVLNQDGVVIRRLEAEKTPEQRALDAARDRDIDARRQHD
                     RFLLTTYTSVKDIEALRDQRLEQIVGQSAAARQYVDSLNTRLTGLQAKAQLYRPYSSD
                     ENARRMPDDLAADLVRTVSELRAQNNILLTKQQERDSVALQFQADMDRFVELQSSIAK
                     H"
     gene            complement(49246..49905)
                     /gene="pyrE"
                     /locus_tag="PKN91_00230"
     CDS             complement(49246..49905)
                     /gene="pyrE"
                     /locus_tag="PKN91_00230"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNS26523.1"
                     /translation="MQEFQRTFIDLALARDALRFGRFTLKSGRESPYFFNAGLFNDGE
                     SLAILGRCYAAAIARAGLGFDMLFGPAYKGIPLVAATAIALAGEHGRNLPWAFNRKEA
                     KDHGEGGSIVGHRLEGRVLIIDDVITAGTAVRESVALIRASGAQPVGIALALDRQERG
                     QGERSAVQEVEADYGLRCISILTLGGLIDALGEAKGDGRRISAEHHAALQAYRDRYGI
                     A"
     gene            49947..50723
                     /locus_tag="PKN91_00235"
     CDS             49947..50723
                     /locus_tag="PKN91_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNS26524.1"
                     /translation="MRIVTLNANGIRSAAAKGAFEWLGRQDCDVICLQETKAQEQQLA
                     DHPIDIPGYHRYFHDAKRPGYSGVALYARRRPDAVSRGFGVSEFDDEGRYLEARFGKL
                     SVVSLYLPSGSSGPERQASKFRFLDAFLPHLAALKRKRRQFVLCGDWNIAHKPIDLRN
                     WKANQKNSGFLPEERAWLDRVFDELGYVDAFREVDPRPDQYTWWSNRGQARAKNVGWR
                     IDYQIASPGLAGSAQRAVIHTADRFSDHAPLVMDYDLKRL"
     gene            50720..52006
                     /locus_tag="PKN91_00240"
     CDS             50720..52006
                     /locus_tag="PKN91_00240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS26525.1"
                     /translation="MTAATAATPAAPRPRFRDVLREPRMIAVLGLGVASGLPYNLTDG
                     TLQAWLKDYGISNTQIGLLTLVGLPYAWKFLWAPFMDRYQLPFLGRRRGWIFLLQLAL
                     AAVIAACALFSPADALTAVAILAFAIGVLSASQDIVINAYTADVAQPHERGLAAMATS
                     VGYRAAAWFSFSIALIVAQFAGWKAAYLMIAAAMLALAIGTLFAPEPRERAAPPDSLR
                     ASIVDPLRQLLSGPGAVALVVLLVVYKVGDAFSLRLFTPFLMDLGFSKAEIGVVTKTT
                     MAVATIAGTVAGGLWMIRLGLFRALLWFGAFQAVSNLTYMMLAVAGKDHVLMIVAIAI
                     DNFAGGMGSVALTAFILALCDQRFSAFQYALLSAIAVIPRTYLGVPAGYLADRAGWPT
                     FYFVSFIAAIPGIALVWYLRRDVRALDARGAPGTVA"
     gene            52003..52389
                     /locus_tag="PKN91_00245"
     CDS             52003..52389
                     /locus_tag="PKN91_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNS26526.1"
                     /translation="MTDIDLSRVLCTLDDLPVDSARDFVVGGGSWPLRGLLVRTREGV
                     HAYLNRCPHARHPLNLRPHEFLTPDASLILCRSHGALFERATGQCVAGPCAGQALTAI
                     AIRVEAGCVLLDEAVDPASFEDVEAF"
     gene            complement(52363..52683)
                     /locus_tag="PKN91_00250"
     CDS             complement(52363..52683)
                     /locus_tag="PKN91_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26527.1"
                     /translation="MMATPFPNIGAWYRLDSGEAFEVVACDTDDGTIEIQYFDGTVEE
                     LDIEDWELHAEERRLEEVEPPEDWSGSFDVDSDFERGALSSDDYEETRGMLAGPLEGL
                     DIFE"
     gene            52777..53400
                     /locus_tag="PKN91_00255"
     CDS             52777..53400
                     /locus_tag="PKN91_00255"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNS26528.1"
                     /translation="MFDQVVKFFVVFFVVVEPVSLLPLFVSLTDGANEEFRRRMARRA
                     VLLSGAILFLFALFGAKFLELMGISLEAFRIFGGLLLFLIALEMVFARESGTRTTSRE
                     QAENRQRADISVFPLAFPFIAGPGALATILLWFGPVSITATPGHWAALFSAVLAVLLI
                     AWVFMRLAERLMRVIGVTGASVSSRLLGVILGALAVQFVIDGLKGAF"
     gene            complement(53435..53605)
                     /locus_tag="PKN91_00260"
     CDS             complement(53435..53605)
                     /locus_tag="PKN91_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26529.1"
                     /translation="MADDSTAELRPQALVDTRQLAERAGRLKSEIKELEKDRARYEQE
                     LEDFRVTVAWTG"
     gene            complement(53595..53816)
                     /locus_tag="PKN91_00265"
     CDS             complement(53595..53816)
                     /locus_tag="PKN91_00265"
                     /inference="COORDINATES: protein motif:HMM:NF022426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HNS26530.1"
                     /translation="MSGNECRTVDWRTIGYEDGVAGHSGDRVAQHRRACAKHGVTPDL
                     AQYQDGRSEGLREYCQPSNGFRVGAGRGR"
     gene            complement(53950..54636)
                     /gene="trmB"
                     /locus_tag="PKN91_00270"
     CDS             complement(53950..54636)
                     /gene="trmB"
                     /locus_tag="PKN91_00270"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNS26531.1"
                     /translation="MKRTIRSFVIRGGRMTDAQQRALENLWPRYGIDTDTSADGSTAL
                     DLDAIFGRHAPRTLEIGFGNGDHLASLAARHPERDYLGIEVHRPGVGRLLLSCEAQGS
                     RNVRAICRDAIEVLEQCLAPASIDEVLLLFPDPWHKKRHHKRRIVQPPFVAAVARVLA
                     CGGVFRLATDWEPYAMQMLEVLDASAEFDNLVPGGGFAPRDPERAATRFERRGERLGH
                     GVWDLAYRRR"
     gene            complement(54633..55766)
                     /locus_tag="PKN91_00275"
     CDS             complement(54633..55766)
                     /locus_tag="PKN91_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26532.1"
                     /translation="MTSRSALLRPCLPLMLICLAGCVTTAPTDPVVIAQPREITTTEV
                     LAEAGDGADRDSVARYRPTEITTGEVQAISAGDIAPPLDFRQRLDRAHDVLYTRFQRL
                     VEATDRRYARPDKELKPVPAAPFRIGVTGELLGRNDKTEASLDLDLDVSLRLPNLEDR
                     LRIFVTSVELDESPEVAGEDSNLRAGLRYQLLKYVSFDIGAKADLPPVAFMAVKWSRE
                     YHLGRWDFYPFAKLFAETDESVGYAAAATFDRWSGRRLLRSSTYAKWRNDRDETEWSQ
                     SLVFALAEQLIVPDRYGSYLRARDIGKGVALHLLASGLDAHGVDYYETGLLIKRPTGN
                     PWLHWFVEPLVRWDRRYDWDADPGIRIGLDALFWDLARWGRAP"
     gene            complement(55827..56228)
                     /locus_tag="PKN91_00280"
     CDS             complement(55827..56228)
                     /locus_tag="PKN91_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26533.1"
                     /translation="MRAITVALTLAVGTIASPALHADEFTKEDLARWEAQFQSVVAEG
                     RALWGDGKLGKNGVACGQCHPNAANTHPETYPKFQKQLGRVAGLWEMINWCLRNPLEG
                     DPLAADDPKMVALLAYATWERRGVKLEPGKH"
     gene            complement(56252..57322)
                     /locus_tag="PKN91_00285"
     CDS             complement(56252..57322)
                     /locus_tag="PKN91_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNS26534.1"
                     /translation="MNLQSRRSFLKSAGTAGAVATLPLSLVEVAFAKPEQNFTFAYIS
                     DSHIQQIEGTKFVRNWDRGLIRAVAEANLLQPRPDFVMYGGDLAQLGNKPELDHGADM
                     LGKLRGKVHHVMGEHDYYLDLGEYWSKLFGPQWYSFDHKGVHFVVLNSILTYDEWTFE
                     RWPTAEQRMLEMAGLDNPNGSPFMVGDKQRAWLAKDLAKVAKSTPVVVFSHSPLQKIY
                     KGWNFWTDDAEQVQQLLAPFDKVTVFYGHVHQIQYNQIGNIAFTSAMATAWPWPYPET
                     YAQAESHLPKLTVHMNRADPFFERDATGWQFIDVHTGRAATHFNLYNNKVRTVAFNAA
                     SGRPEDTAYQDPAARIAPQSHF"
     gene            complement(57319..58491)
                     /locus_tag="PKN91_00290"
     CDS             complement(57319..58491)
                     /locus_tag="PKN91_00290"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNS26535.1"
                     /translation="MHAPRTLWFVAACAAAAWAALPATAAAAGTAGPALGLPPVPVPA
                     DNPQTAEKIALGDKLFNDKRFSATGEVACSFCHDSQKGFADGPLVTAEGINKAKGTRN
                     SPTVLNSAYFTSLFWDGRSPDLEEQALHPFVNPIEMGLPNHDPILAIVRTDPEYVAAF
                     SAVFGKSGEAITIREVTQAIAAFERTLVSGDSPFDRYYFGGDKSALSAAAIRGFDVFL
                     SKGRCVSCHTIEQDHALFTDNRFHNIGVGVNRIQGDIGTLAPAFLRAKAEGIDVDKVV
                     LADPKVSELGRFAITDTLDELGAFKTSTLRNIAITAPFMHDGSLATLRDVVDHYNNGG
                     VTVATDPVNDFLSGGIRPLDLTESEILDLVAFLESLTSPEFAATPSAGGTTKGTQP"
     gene            complement(58611..59219)
                     /locus_tag="PKN91_00295"
     CDS             complement(58611..59219)
                     /locus_tag="PKN91_00295"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNS26536.1"
                     /translation="MSGAIGPAGIAILGTIVVLSLFGLYGSPAFLERCLFRPYWFLRK
                     RDYPTAVLAGFVHADLPHLLFNSITFWFFGIPLERLIGTRPFLVLYFAGLLLSQFGTW
                     AKHRDNPDYATLGASGAISAVLFASIVYLPTQSIFILPIPFPIPAPLFAVGYLAYTWW
                     SAKHARGRINHDAHFAGALTGLAFVALAHPQAYSRAFALLTN"
     gene            59375..60181
                     /locus_tag="PKN91_00300"
     CDS             59375..60181
                     /locus_tag="PKN91_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNS26537.1"
                     /translation="MKVRRSYVDGRYGQMHVRSAGDAGLAHRPLLCFHLSPVSGAIYD
                     KWLAEMGCDRLALAPDTPGYGMSDPPPAVPTIADYAAAMGDLVDALALGEFDVMGYHT
                     GSKIAVELARQRPAQVKHLVLMSTPIYTPEDFAAIRELTSVAPIAADGQFLVDRWKKH
                     LLYQDRRATLESSMRNFPWHLMGGERSDWGYRAAFGYSYPDNIVQVTAPILVFNPEDD
                     LCTYTPRVRPYLKNGMVRDLPGWSHQLLDFSTGEMATMVRGFLDEDRFPA"
     gene            60191..60361
                     /locus_tag="PKN91_00305"
     CDS             60191..60361
                     /locus_tag="PKN91_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26538.1"
                     /translation="MDQISRITITIAFIGLAGLIVGFTTRDRPYGTYLMWAGVMCMIG
                     VVVYHIVKTVQS"
     gene            complement(60396..61733)
                     /gene="gdhA"
                     /locus_tag="PKN91_00310"
     CDS             complement(60396..61733)
                     /gene="gdhA"
                     /locus_tag="PKN91_00310"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNS26539.1"
                     /translation="MEARIQQIYDQVIRRNPGEVEFHQAVKEVVDSLGPVLAKHPEYA
                     EQKIIERICEPERQIIFRVPWQDDRGEVQINRGFRVQFNSALGPYKGGLRFHPSVYLG
                     IVKFLGFEQVFKNALTGMPIGGAKGGADFDPKGRSDGEVMRFCQSLMTELHRHLGEYT
                     DVPAGDIGVGAREIGYLFGQYKRMTNRYESAVLTGKSPSWGGSLVRREATGYGAVYFV
                     DEMLKVRGQSIEGQTCVVSGSGNVAIYAMEKLQRLRGSVIACSDSGGVIYHKSGIDLD
                     LVKRLKEVERRRISEYAQHHRDARYIAGGSVWDIPCEIALPCATQNELDEAAARKLVA
                     NGCIAVAEGANMPTTPGALRVFQEAGIAFGPGKAANAGGVATSALEMQQNASRDTWSF
                     EHTEQRLQEIMSGIHRNCHEAAAEYGSPGNYVLGANIVGFRRVAEAMLAFGVI"
     gene            complement(61806..62273)
                     /locus_tag="PKN91_00315"
     CDS             complement(61806..62273)
                     /locus_tag="PKN91_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26540.1"
                     /translation="MSGANATMDLLRQHVGIWEGDYTHIDPADRSVQESYAFRIKVEC
                     PDYGPIAYRQTSRYRWDDGRTTELVYTGIAQGDRLLIDDGRVRGEIRAIEPQTLYMRF
                     GYTADPGNQVTEMIQLSRDGIHRARTWHWLRDEQLWRITLVREQRKSRDLADW"
     gene            complement(62270..63247)
                     /locus_tag="PKN91_00320"
     CDS             complement(62270..63247)
                     /locus_tag="PKN91_00320"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNS26541.1"
                     /translation="MSRDLNDTLVFVKVVEHGSFIGAARALRLPKTTVSRKIQDLEAR
                     LGTQLMHRTTRKLGLTEAGNVYFEHSQHIARVLDEAESAVGQLQSGPRGWLRLTAPFS
                     FGVTWIAPLLGEFYLRYPEVRVEMELTNESLDLIDKEFDLALRIGRLNDPSLVARRLI
                     SFRTQVYANPTYIAQHGEPHHPDELVHHRTFAMSSYHRNSGNDYVWRLSNGAHKADFP
                     IDPVLVSNDPAALRGALLSGEGLMLGADVMVKPDAAQGRVVRVLAGWEGPTLDMNAVF
                     LRGQGKSPKVRAFVDFLVERLSVDADFMEAICPHGKCQESVEPARADAA"
     gene            63489..64679
                     /locus_tag="PKN91_00325"
     CDS             63489..64679
                     /locus_tag="PKN91_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNS26542.1"
                     /translation="MLAGHLPWTGAALVLIALVGTTLVAFGSGANHDAGIGHMRPPDV
                     SVATVLTQSVQPWDEFTGRITAVESVELRARVSGYVERVAYEEGQFVRKGDLLFLIDQ
                     RSYRAEYERARAELARARSEAALARSQDERTRALVAARAASQEESDERRAAADQSLAA
                     VRAAEAALERARLDLEFTEVRAPIDGRAGRALITAGNLAQADSTLLTTVVSVDPVYVY
                     FDADEQTYLRYQGLARSGERAASGNAVRIGLATETGFPHAGTVDFIDNQVDAGTGTIR
                     VRALVPNPGLLLTPGLFARVQLQGGEERAALLIDDRAVLTDQDRKYVYVLGPGNAAVR
                     KDVTLGRTSGDLRVVTAGLEAGDTIIVNGVQKVFFEGMPVSPQLVAMGESSSGAVRVA
                     AGSN"
     gene            64689..67832
                     /locus_tag="PKN91_00330"
     CDS             64689..67832
                     /locus_tag="PKN91_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNS26543.1"
                     /translation="MDFSRFFIDRPIFAAVLSIVIFAAGLIALPGLPVSEYPDVVPPT
                     VIVRAVYPGANPKVIAETVATPLEEAINGVEDMMYVKSVAGSDGVLLTTVTFRPGTDP
                     DDAQVAVQNRVSQALARLPEDVRRQGVVTQKQSPTLTMVVHLTSPDRTYDSLYLRNYA
                     TLKVRDELARLPGVGNAQMWGSGDYAMRIWLDPDKVAARGLTADDVVRAVREQNVQVS
                     AGQLGAEPAPNGSDFLVSINAQGRLQTEEEFGRIVVKSGADGELVRLADVARLELGAG
                     DYTLRSQLDNQDAVAIGIFEAPGANAIAVSDAVRARMADLAKRFPPGVAYSVVYDPTV
                     FVRDSIKAVIATLLEAVVLVVLVVILFLQTWRASIIPLISVPVSIVGTFAALHLLGFS
                     VNTLTLFGLVLAIGIVVDDAIVVVENVERNIAEGKTPLAAAHQAMREVSGPIIAIALV
                     LCAVFVPMSFLEGVTGQFYRQFAVTIAISTVISAVNSLTLSPALAAKLLRAHDAPKDA
                     PTRLIDRLLGGVFRPFNRFFGTSAARYQAAVAHSLHRRGAVFAVYAVLLVAAWGMLQA
                     VPGGFIPTQDKLYLMGGVKLPEGASIERTDAVIRRMSEIALGTEGVASAVAFPGLNAL
                     QFTNTPNTGVVFFGLEPFGERTRSAAEINAEINAKIAGIQEAFAFSLMPPPILGIGAG
                     SGYSLFIQDRAGLGYGALQEAVSRFSGAVARTPGMGFPISTYQANVPQLDAQVDRVKA
                     KAQGVPLTELFDTMQTYLGSTYVNDFNRFGRTWQVIAQADASFRDEPSDIARLRTRNL
                     AGEMVPIGSVVTLGETYGPDPVMRYNGYPAADLLGEADPRLMSSNEAMAAIAQIAPQV
                     LPEGMTMEWTDLSYQQATQGNAALVVFPLAVLLVFLVLAALYESWTLPLAVILIVPMT
                     LLAALTGVWLTGGDNNVFVQVGLVVLMGLACKNAILIVEFARELELAGRGVVEAALEA
                     CRLRLRPIVMTSIAFIAGTVPLVLAHGAGSEVRTVTGITVFAGMLGVTLFGLFLTPVF
                     YVALRRLAAPRLELAHA"
     gene            67825..69252
                     /locus_tag="PKN91_00335"
     CDS             67825..69252
                     /locus_tag="PKN91_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNS26544.1"
                     /translation="MRRHRLMLVLTMAMALTGCAVGPDYVRPDLPVPGSFTVAPAAAP
                     DSAGEVVLAPAVDPEFWRGLGDPLLSQLVDAALESNHDLRIALANYDRANALLRGARF
                     DRYPTVTAGGSAASERATADAAPGASREERDLDRYGADIRAGWELDLFGRVRRSVEAQ
                     RAERDAAASDLAALQIVIVGEVARSYMELRGLQERLRVARANAVNQRDTLELVEARVG
                     AGRDTDFDRSRARAQLETTLARVPALESAVAVTLHRLAVLTGRPPAALTAQLASAAPL
                     PALPASVDAGTPGELLRRRPDVIAAEARLHAATARVGIATADLFPRFTLAGLIGTQAI
                     DTDALFERDSEVRFAALGVDWSFLDVGRVRARIAAADADAAAALAGYEQAVLLALEDT
                     ENALVRLAKARLEDRHLESAALDSATAARLARLRFEAGAVDLLDVLDAERTQLLAQDA
                     FADARTRSATAAVGLYRALAGGYGR"
     gene            69313..69386
                     /locus_tag="PKN91_00340"
     tRNA            69313..69386
                     /locus_tag="PKN91_00340"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69345..69347,aa:Gly,seq:ccc)
     gene            complement(69436..70008)
                     /locus_tag="PKN91_00345"
     CDS             complement(69436..70008)
                     /locus_tag="PKN91_00345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HNS26545.1"
                     /translation="MSTYTVGYLIGSLAKGSINRQLANALVKLAPKELRFHEITFNEL
                     PLYSYDYDADFPEVATRFKKAISESDAILFVTPEYNRSIPGGLKNAIDWASRPWGTNS
                     FARLPSGVIGASTGAIGTAVAQQQLRSVLSFCNSPQMNAPEAYIRFTPGLINDKFEVT
                     DSGTEKFLRGFMTEFHQFIARVRTVLPREG"
     gene            70125..71297
                     /locus_tag="PKN91_00350"
     CDS             70125..71297
                     /locus_tag="PKN91_00350"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNS26546.1"
                     /translation="MTTTKLLNLGAIVALLAACGGSGNEAAADAVPAATGNAPAAMSF
                     DGFVATPVARFDKPWAMTFLPDGRLLVTEMAGVLKLYDVAGNRTGIVSGVPAVFHKNQ
                     GGFSDVVLHPQYSTNQLVYISYSEAGDGGAGAAVARARLVLDAAGGGALEGLEVIWRQ
                     VPKKSGAGHFGQRIAFDRDGMLWISSSERQEFDPAQDLDTNLGKIVRLNDDGSVPDGN
                     PFADRGGVSAQIWTLGHRNILGLAFDAQGQLWAHEMGPKGGDELNRIERGANYGYPIV
                     SNGDHYDGRVIPDHDTRPEFRAPVVSWTPVIAPAGFVFYDGDLFPEWKGSAFIGGLVS
                     MSLVRIAFDGDSAREAERFNMQRRIREVEQAPDGALWLLEDGNRNGQGWLLKLTPR"
     gene            complement(71301..72392)
                     /locus_tag="PKN91_00355"
     CDS             complement(71301..72392)
                     /locus_tag="PKN91_00355"
                     /inference="COORDINATES: protein motif:HMM:NF022825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HNS26547.1"
                     /translation="MTRWIALTVVAALAQTTAMAADDTGFYGPLRARDLTPFGFLRLD
                     MRPVLGGFGEPGAWAVATEVAYQNTWALSRETEKYLGRLPGRRQLGPAEFQAIRDLPG
                     ENFLIDLELAQFDMTLYYQLSHAWSAYLILSGATYQGGFLDGMIERFHDAFAFSNFGR
                     PAANRNDVNILLDLKSSQYAAYEAPTSGGLLDPTLGVRYSGIAMPENWKLVLEGAAKI
                     PVAGRREMLSSGRVDFGVQATLQRYWQRQAFYANLSGVYYAGTGGFMPSDAQVIPTLV
                     LGYELKLSARTNAIIQGYASPSVYTRADTDLDELTATKFQISLGVHHRRGAHLFTFAA
                     TENLQNFNNTPDIGFQLGWAYVAGRPLRP"
     gene            complement(72445..73344)
                     /locus_tag="PKN91_00360"
     CDS             complement(72445..73344)
                     /locus_tag="PKN91_00360"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNS26548.1"
                     /translation="MDRFRAIEAFAKVVEFGGFARAAERLGTSTSAVSRLVAELEAHL
                     DARLINRTTRRLSLTEAGQSFYERSVPLLLDLEEAESSVRASAVLPSGSLRLSCGVTF
                     GERFLAPAISEFAALHPQLVFDLDLSDRQVDLVEEGFDLAIRIGAIAHQGLVSRRIGW
                     TYPLCCASPAYLERHGTPRTPEELGAHAILTYTLMPLPNVWRFESAQGTRHELRVATR
                     HRANSGRVLAALAASGLGVVIEPDFIVAPELQTGALVRVLADYALARSPVSAVYPSRR
                     HLSAKVRSFVDFLAGYATRTLAT"
     gene            73457..74062
                     /gene="gstA"
                     /locus_tag="PKN91_00365"
     CDS             73457..74062
                     /gene="gstA"
                     /locus_tag="PKN91_00365"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HNS26549.1"
                     /translation="MKLYYTPGACSQAPHIAARELDLPIDTVKVDLATHKLADGTDFY
                     TINPKGYIPLLALDDGTTISEANVILQYFADQKPGTLAPVFGTLERWKLMELLSFIAS
                     EVHKGYAVLWNPQTPAEVRKAAIDRLGTRFDLLAKTLATQPYLTGGSFTIADAYLFVL
                     LNWTKIHKIDLTPWPALQAFQARVAARPAVQATLKAEGLLK"
     gene            74066..74851
                     /locus_tag="PKN91_00370"
     CDS             74066..74851
                     /locus_tag="PKN91_00370"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HNS26550.1"
                     /translation="MTTRLPTLFLSHGSPMHALDAGGAGPAWEALAASLPRPRAILIA
                     SAHWETTLPMLTGGERLETLHDFSGFPPELYRVRYDVPGAPELAARAGQLLKDAGYTP
                     AINACRGIDHGAWVPLKWMYPARDVPVVQLSVQTALGAAHHLGLGEALAPLRDEGVLV
                     IGSGHVTHNLRDWAAHRGDRQPADYVVQFPAWLNERLLRRERDELLAWQERAPSAARA
                     HPTDEHFLPIFIALGAAGADAAPTRFYTAIDAGVLSMDAYRFD"
     gene            complement(74856..75431)
                     /locus_tag="PKN91_00375"
     CDS             complement(74856..75431)
                     /locus_tag="PKN91_00375"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNS26551.1"
                     /translation="MSQLGRILLKGLVAILPIGLTIYLIYWLGITTEKLLSGPIHWFV
                     PPADYIPGMGLVAGFIVLLIVGLLVNAYVIRRGMKVVENLVMRVPVVKTVYGALRDVT
                     KLVDTSGRKSELERVVMVKFGPGRVMGFVTQEHAALPGIDTDGDLVAVYLPMSYQIGG
                     YTVYLPRSQVEPTDLSVEAAMRIVLTGGLQR"
     gene            complement(75514..76044)
                     /locus_tag="PKN91_00380"
     CDS             complement(75514..76044)
                     /locus_tag="PKN91_00380"
                     /inference="COORDINATES: protein motif:HMM:NF008437.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HNS26552.1"
                     /translation="MNRQTMIGAVAGAVLVTGVAALAGYRVMSNDRYAEVLSVEEIQR
                     TERTPREVCRDEVVTQQKPVKDKHQVTGSVVGAVVGGVLGSQIGSGSGRDAATVAGAA
                     AGGYAGNKVQERIQEGNTEQVTVTRCETVFDAKQVPTGEFNVRYRLGDAEHTVKMDHD
                     PGSRLPVKDGKVVTGS"
     gene            complement(76114..77226)
                     /locus_tag="PKN91_00385"
     CDS             complement(76114..77226)
                     /locus_tag="PKN91_00385"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNS26553.1"
                     /translation="MSRYVPLIVAALLAAAALIAAAALWRRSRAARTLPAAPASSAPP
                     AAARVATAPGAPAPGDAWVALHRLALSAPQLEPQVPAGHEEAAAWFAEGIEACAAQDR
                     YMPRRPMLLPQLVRAVNDTETSRRELAGIIARDPALAADLLRLANSPYYRMSRKPIES
                     IDRAVAVLGTEGIRRLVAAAVLQPVFRAQGGHARFPDTTWDHAFRSGVAAESYAAIVD
                     GSDPFAAQLLALLMGLGGIMVYRMAFDRYAGPTQATPPPALIAAMIDRHAARAARAVA
                     ASWGLSERALAALEEQRADADARAMSPLGRSVWFGRLTGALAVLRSSGAMTDDAALAT
                     LRAAGTPAQQTERIWMRLVPEAIIAAPEAARAPARM"
     gene            complement(77234..77908)
                     /locus_tag="PKN91_00390"
     CDS             complement(77234..77908)
                     /locus_tag="PKN91_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26554.1"
                     /translation="MFEESLGYLVVKFAHILLFVYWLGGDAGVFYSSGFVVNPKLSRD
                     ARLTAFKIFAELDMLPRYCLALMLSVGGLLAWYIDVPHLPGQLPLILLLGPLWVLLVW
                     AVHHYQGQALGKRLTQFDFYFRCFMIVALIASVGYAYGDGRIRPYPWLAAKLLIFAFL
                     IFCGLMIRLKLPRFVEGYKQLLTSGATEESDRNMSEGLRACRPYVLAIWVGLLAAAFI
                     GIAKPA"
     gene            78066..>80770
                     /locus_tag="PKN91_00395"
     CDS             78066..>80770
                     /locus_tag="PKN91_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNS26555.1"
                     /translation="MSTPLHAAIPGDLAPGLFRDMAEQWPGGVIVVNRDGVIRWLNAA
                     AHDWAPAGAGALVGACIDSMRLPWLQDHHDLAMVLGGGSQQLAPRAVIDRHGRERYCG
                     LRLMPLRRGQAICGAMLFVEEGVAVDGSTVVQAPSISDTARAIALSASQVGAWHCNLQ
                     SGEGGVDAAWCAALQLDPCTGPGHLARWAKHVHPDDLAAYREGWDEFAQGASDSFEAE
                     FRALTDDSRWMWLLQRGQVVEHDEDGRPLRAAGICIEIDARKRAEVALQEKESRLATA
                     LWGARAAFWQWNITSDVSARSAMWFAMTGYQREEWERETRPWHSRLHPDDAERVERTV
                     RDHFEGRTQSLEIEYRIRVASGEYRWVQDRGRVTEWDFQGNPTIAIGVSLDIESQKQA
                     ELELRASEARLETAVWGAKIGLWEFDFETSHARWYNDWCDQVDFDACEGADHQSRWEA
                     NIHPDDRAEVDRSFDEHLAGRIGHYDAEYRVRTRSGEWRWIFERGRVTARAPDGAPLR
                     MVGVCMDIEERMVAARDAREVRERLERAIDVARIGIWSWDVVDGVFHCNELYADYACG
                     GKVPRDAAERQRVRVAHLHPDDLGEIRALEESVHSGRQDEFECRHRVRHAQGDWRWVL
                     QRGRVTARSADGTPAHMAGFLVDVSDSYEEVMTREKAVAKLQTLAEHTPDYLLLVDRD
                     LRVQIANRGWGAYSTADIVGRSVLDFVPEANRQVLTALYTTVLATGISDERTVGQPRA
                     DGSGMRLFVHRASPVRENGVITGLTLAMTDATARLQAEERSRATESVLKTIAEVTPDW
                     LVMLDENQRCTFINRSIAGHEPGEIIGRRLDELPVRVLGDGVRELLQGVLRSGRARTE
                     EHDFHAPDGTSRRFELRVRPVHAAAAGSGTGRGLVVTASDVTTRKT"
     assembly_gap    80771..80780
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <80781..81437
                     /locus_tag="PKN91_00400"
     CDS             <80781..81437
                     /locus_tag="PKN91_00400"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNS26556.1"
                     /translation="ALEREILEIANREQQRMGRDLEDGLIEDLTGIALQLRAVAGQLR
                     KEGSEARADVEDAIGQVNSAIEGTRVLASGLSPVGAERGGLVAALEALAARTRDRHGV
                     QVDVVTQLATPLVLVDGALNHLYRIAQEAVINAVRHGHPRHVRIELALDGGRVRLRVT
                     DDGRGLGPLAAQSAGLGRRVMRYRAQMLDGELTVRPGESGVVVECICPVANNQGAAAF
                     "
     gene            81458..82075
                     /locus_tag="PKN91_00405"
     CDS             81458..82075
                     /locus_tag="PKN91_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNS26557.1"
                     /translation="MRQGLVRMIEAAPDLSVCGEVENVVDARRAIREHQPDVVIVDLT
                     LGQGDGLELVREVRAHHPRLPMLVLTMHEEAIYAERMLAAGARGYIMKEAASEQLLTA
                     LRRVLSGRVYLSDAMATRLGERGAMPEGETADDPLARLSNRELQVLGKLGRGMSSREI
                     ASHLAVSVKTIESHRQSIKRKLNLTSNAQLLQYALSFVASTRGGV"
     gene            82143..82715
                     /locus_tag="PKN91_00410"
     CDS             82143..82715
                     /locus_tag="PKN91_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNS26558.1"
                     /translation="MADGGTAGARAVAVADIEDALVARARRGDARAFERLYREHAGRV
                     HGLCLRMARDPQLAEDYTQETFIRAWRALDRFEARSRMSTWLHRIAVNVILERRRRRD
                     LPLEFVEDAAEYDRDNDTLDTPVEEAELEAAIGSLPPGARDVLTLCGIYGYTHGEAAA
                     MLGIAEGTCKAQLHRARALVRARVASGEAR"
     gene            82712..83296
                     /locus_tag="PKN91_00415"
     CDS             82712..83296
                     /locus_tag="PKN91_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26559.1"
                     /translation="MNEHERKVASPGELPQEIPPARDLWPGIAAAIGAADDVRAVTAP
                     LEPARRRFAVHPLLGLAAAISCVAVGMWLGRVSAPPVTVAAAPGEPGAAPGGAAPVPA
                     ASLALDARFDRAREELLRDVAERLAALPPESRAKVEASLADLQRARTDIEAALGRDPG
                     NALLQAMLVNSYQDEMRVLAAVRDTWGYQEDVGA"
     gene            83293..84198
                     /locus_tag="PKN91_00420"
     CDS             83293..84198
                     /locus_tag="PKN91_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNS26560.1"
                     /translation="MSMQLMRSALGVALAALLLATPAMARDDRTVDERIAADADGEVV
                     VSNVSGSVRVEGWAGKEVEITGSLGAGVERLDVVRDGKRVVVKVVLPRTSSHRSSADL
                     RVRVPVASRLEVTTVSADSSVRGVTGALRLSSVSGEIEARGVASDSEIKTVSGDIRVT
                     AASTPIKLRATSVSGTIVVDDLSGEVETLTVSGEMLLDLDALTNVRARSTSGDSRISG
                     QLARDARAEFESVSGDMSLKFGAAAGFAVDAESFSGELSNCFGVKAEAVSKYAPGERL
                     SLTRGEGSAQVRIKTMSGSVRICDR"
     gene            84215..85492
                     /locus_tag="PKN91_00425"
     CDS             84215..85492
                     /locus_tag="PKN91_00425"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNS26561.1"
                     /translation="MRVAKWVLIGIAGLLVAVALAVLVITQFVDPARFKGPIERQVAA
                     ATGRDFKLAGDIELSFFPWLALTTGAAELPAPAGFADPRFLAWRNAHVGVRLLPLLRG
                     ELVVDRVRLAGLDARLVKAADGRENWAFGDANGEDSGDASGEGTLPDIAGIELREARV
                     TYIDEAAGTQFELADARIDIEPIRSGVPMRIEAAATASMADLPERIAVELESRVTLGP
                     PIVLADTSLSGTLREGRFGAAGVPWRLAAPRVSYDAASGALAAPEWQLRFNDAQLDGE
                     LAARLGEPLTAEGRVSLAPVSLRETLAAAGVELPPTRDKAAWERFGFDAVFRVSGPAF
                     TVSPLAIRLDDTSLGGTIERAALEDSLIVFELRADRMDVDRYLKPAGTPSEPFVFPAE
                     ALKALRARGTLAIDEAALEGVRLRGVVFEADTP"
     gene            85489..86532
                     /gene="mutY"
                     /locus_tag="PKN91_00430"
     CDS             85489..86532
                     /gene="mutY"
                     /locus_tag="PKN91_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNS26562.1"
                     /translation="MTGIADALLPWYRVHGRHDLPWQRERSPYLVWVSEIMLQQTQVA
                     TVIPYFERFVGRFPDVTTLADAPLDEVLHLWSGLGYYARARNLHRAARRVRDEFAGRF
                     PGTFAEIESLPGVGRSTAGAILALSRGERLPILDGNAKRVLARRFGVTGSPGERAVEQ
                     RLWALAEENTPHTEVDAYTQAIMDLGATVCVRRRPLCPICPLRENCVAYRSNRQHVIP
                     APRVRARSARRVREVVMLVAQREDGAVLLERRPAEGIWGGLWCLPEFASETAARGYAA
                     SQLEGAETDARALAPVPHVFTHFDLVITPLLARCRDGGRVADEDRTLWYDIRSPARVG
                     LPAPIQLILASLT"
     gene            86529..86798
                     /locus_tag="PKN91_00435"
     CDS             86529..86798
                     /locus_tag="PKN91_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HNS26563.1"
                     /translation="MSRTVTCVMLKREAPGLDRPPYPGTLGQRIFEQVSKEAWARWVG
                     HQTMLINEYRLTPVDPKARKFLEQEMEKFFFGEGSVVPEDYRPPR"
     gene            complement(86889..87434)
                     /locus_tag="PKN91_00440"
     CDS             complement(86889..87434)
                     /locus_tag="PKN91_00440"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HNS26564.1"
                     /translation="MQKTLLDFWWVPIVRGTVAILFGILVFAWPGISLLAFLMLIAAY
                     WIVDGIASIYYAIKATNWGWPFWSGVISVAAGGVAVLAPGLAAISILIVIAVWAIAHG
                     LLDIYTAIRFRKEIDFEWWLGLSGAVSVIFGVLVLRQPAAGALAIATLIGAFALVIGV
                     VLVISGLRIRAFRNKRRPMGV"
     gene            87597..87672
                     /locus_tag="PKN91_00445"
     tRNA            87597..87672
                     /locus_tag="PKN91_00445"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:87630..87632,aa:Phe,seq:gaa)
     gene            88003..90492
                     /locus_tag="PKN91_00450"
     CDS             88003..90492
                     /locus_tag="PKN91_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS26565.1"
                     /translation="MKPTYLRRAVATAPVIRAALRQALGVSVALLLLSPAQAQDEPAS
                     QNAQAQEANGLAEVVVTARKRDEALLDTPLSVQAFTSEDIASAGLRNLEDVSAMTAGL
                     NFQKLGNSQAGRYNSAIRFRGLELLVTTPTSQTGSLFIDGVYILGGAASMQFTDVERI
                     EVIRGPQAAYFGRGTFGGAINYVTADPASSFGGRTSFEYSPNFGSTGINGSIEGPLGD
                     TLSGRLSVSSSKRGAMFTATDGGALGEERTDMITGTLLWQPSDALKVKVRALYGEDDD
                     GPASSTFVPFRLYGNCPIGTPITVKTTTGEVATTMRRDLHCGSVPVVPVSNNTTFYTV
                     DTTLGPQNTYDIMVLNNGLPPTDTPAMDHFGLRSIFHLYTGSVDYDFNDALTMSVLLG
                     YNKRDTTQIRDDDSYDTMGRVTKGFLSLESTSVEARLYYDGGNRLRGMIGVNYFDEES
                     FGDIDGGIGLVTNLLGPPSIGSAGGSIDAGKTDTSAVFASVEYDLLENLTLSLEGRYQ
                     DIDTETLGGAYPGPYTTTKSKHNNTLPRFLVNFKPFESTTLYANYSEGALGGSRNTAF
                     AIRTPAEKEVILAQVPGVSEQLAQQKLESYEIGWKQSLGSSRSWFSLSAYQMDWTNIP
                     GSAQVTFISPTTGNAVSIGVTLPGDAELKGIEAELRWQPLDQLALHASGGYIDAKYTD
                     FISAGLNSYFGLGGALGSYKADGATLPRSPQETFALSATWTDTLNSDWDWYVRGDATY
                     AGKAYTDETNLSWIGGYSLLNSRIGFARGDGLNIELFCRNCLNEDGWRTGRRAIDWFD
                     TAPLPNFLTRVGSLSQPMEKREIGLRVQFEF"
     gene            complement(90644..91603)
                     /locus_tag="PKN91_00455"
     CDS             complement(90644..91603)
                     /locus_tag="PKN91_00455"
                     /inference="COORDINATES: protein motif:HMM:NF020468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1838 family protein"
                     /protein_id="HNS26566.1"
                     /translation="MLILTGSLAAGLSAVPQAFGAANASVPAAFKGPYIDLTTPKGNV
                     NAAVRIDANLDESKQKWASVSGIVCGVRDGAALRDLFGFEVVSVARAWGQPDGSYRVL
                     HREAIFYSDLATGDILRSWKNPYIDEVVEVVDVINDPWNHFFAESIRVGGPDYGGLNP
                     QAERKSVPFSNNFFDAGNGMVQNRQSINLYYKAALQPDKWPRESAGPMNRVSEFFTTT
                     VKLADLQDRNKTSVLHTGSWARVTPWLPWMLMGQAPGHILYHSTVQSFDSLDGFKPKV
                     RAHCEKHYPHMLEAPPREYWDKPNLSSLEVYAKTQKPKPPKKP"
     gene            91888..92589
                     /locus_tag="PKN91_00460"
     CDS             91888..92589
                     /locus_tag="PKN91_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNS26567.1"
                     /translation="MSRPWTGIVPEEDIRRYEAAGFGRPTGYGARPALLIIDVQYRTV
                     GTRPMPFWDAIEEFRTSCGEVGTNAVREIAPLLKLFRDNGHPVLYPHVAPKLSFDSGR
                     LGDKVPAIMNVSAQGYEFVPTIAPTKGDILVPKKHPSAFFGTPLVSYLINLEIDTLIV
                     TGCSTSGCVRATVVDGFAYNFKVVVPEECVFDRSNTVHAVSLFDMSYKYADVRPVADV
                     ISEVAGLPARRPHPG"
     gene            92638..93645
                     /locus_tag="PKN91_00465"
     CDS             92638..93645
                     /locus_tag="PKN91_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNS26568.1"
                     /translation="MEKIPVTLLTGFLGSGKTTLLNGILGLKDFADTAVIVNEVGQIG
                     LDHVLVRAGADNIRLLESGCLCCAVEGTLTETMMDLWSLRHRGDIPAFGRVIIETSGL
                     AEPAPIARSLIQDRFLSSHFVLGRILVTIDALDGIRQLSSFHEAVEQLAIADAVILTK
                     TDLVHGDVAELTEAIRRVNSTAPIIRASLGAIDPSVLTGSSSFTLETPASSQHVHSDF
                     VCRAAYVDDPVSWSGVAAFVDLCRRTYGAELLRCKGLLRIDGEGGAVLLQGVNGAFST
                     TTLERWPDSDPRSRLVCIFRGEPDRFESMLTLLHTEAGTMPPASLDELEQGSMACALI
                     R"
     gene            93627..94991
                     /locus_tag="PKN91_00470"
     CDS             93627..94991
                     /locus_tag="PKN91_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HNS26569.1"
                     /translation="MRFDTLISNGILVCPDRGPFAADIGVTGEHIAAVMSPGQGAQAR
                     EIIDASGQHILPGLIDTHVHFGFAEPVTEYTSETIYAAQGGFTTVLGYFLGSRSYRDV
                     FAEELPLATERAFVDFGFHFCAATERHLEELAAYAHDLGVTSFKYFMNFKGSEGLYLG
                     VEGTDDGYFYDLLAKAASLGEVTVVVHTENIELVNRMRQKFQSGGRNTLRDWSLAKPP
                     ITEAEPTVRAMMLAEHLGARIYIPHLSTRSALNEVRRWRERYANIFVETCPHYLTHTM
                     DADIGSMGKANPPFRSADDVAAMWEGLADGSIDVVASDHVPRKRSTKDKPLWQASQGF
                     PGTATILPILLDQGYHRGRLSLQRIAQLLTSRPAEIFGIAESKGRVAPGLHADLTLVN
                     LDFERVVRAEELGSYSDYSLYDGWRLKGWPRRTIVRGATVMQDGVMTGAGGHGRYIRR
                     AAAS"
     gene            complement(95099..96379)
                     /locus_tag="PKN91_00475"
     CDS             complement(95099..96379)
                     /locus_tag="PKN91_00475"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS26570.1"
                     /translation="MLLLVAYAFAIVDRIIIGLLVDDIKTDLGVTDTQIGILQGFAFA
                     ILYTLAAIPVGVLVDRWNRVKLLTIGIGVWSVMTIVSGFARSFGGLFVSRIGVGLGEA
                     TVTPASSSLISDYFPPEKRQQAYGVFLVGGAIGAGMAYLLGGFAIAIGGPMQEWFPTL
                     LGGFSTWHITFILVGLPGLLIALLMLFTVREPVRRERATATQKFSFDAILRQYRRHPI
                     AYAATYVGAVLNIAIVYAQLGWVPTAFIRLYDWTPTQIATVLSMVSVPCAVISALSAG
                     WFLSWFLKRGRTDGPILVAAMQGGVWAIFGTLKFMMPTGELAMFFHAMTALTGSMGVA
                     AALAGISQITPNELRGQFTAIYALLTGLVSVTAGSFIVGYLSDNLFPGTRGVGMSLST
                     VYFAFGVLAFVALFIGRRDFQAATVHARQWVNKG"
     gene            complement(96782..97783)
                     /locus_tag="PKN91_00480"
     CDS             complement(96782..97783)
                     /locus_tag="PKN91_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNS26571.1"
                     /translation="MLSSVRMFALLLASLIGAAPVIGMAQSPDTAYPGEPKSAGTITY
                     DEIVKRYADQNSRFVVLEGSDGIRVHYKDQGTGPAILLVHSSVGDLVDWDGWVKVLSK
                     SYRVVRLDLPGFGLTGPVPSGNYSVDRYLMLVDALMDHLGIERFAIAGTSFGGLVAFR
                     YAGTRTDRVTALVLQNSAGIEYGGRGGTTERARSPARVFTPEVTTTDMISSTLKSIIN
                     DHSKITPVMVQRKVDYWNVIGRDREGFIATRMYERGNPQRVLGHVRAPALVLWGGNSN
                     ALSPETAQAFVDALKNAKSVKKIIYPGGGHLLHIERPEETVADVKAFLDKEIGKASK"
     gene            98457..101414
                     /locus_tag="PKN91_00485"
     CDS             98457..101414
                     /locus_tag="PKN91_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNS26572.1"
                     /translation="MKLSVILWGIPQAMRICAAVYPKFAQRLKERDCIAQFQLKDQPK
                     GRWIQLKGGKISSRAGIHPSPDFTLFFKNEKIASSFLTPPFDQLERIDAAKNFKITMQ
                     GPDALVDWFMALISSMESYTWTSGTAMGNGVTRYANGTNGGPIFVYVKDGKIIRTTPM
                     DLDDSDAGSYTIKARGKTFVPPRRTTLAAHGQCQKSMVYSKNRILKPMKRVDFDPHGE
                     RNIQNRGKSKFVEISWDEALDIVAGEIKRCKAVGPGAICVANGSHHQWGNFGHYLSAA
                     NRFWNLIGRTRLMHNPDSWEGWFWGAMHHWGNSMRLGAAEPYGTVEDCLQQADMIVFW
                     SSDPDATYGFEGTERRRWAKELGIKMVHIDPYMNHTAAHLGGKWFAPKPGTDAAFAQA
                     LCHVWITEGTYDKEFVEKRTTGFAEWKAHVLGEDDGIPKTPEWQEGETGIPAREVRAL
                     AREWGRKKTYLGAGGWGCGVGGAGRGPTGTQWARMMTILGAMQGMGRPGSNFGNLQFG
                     APVDMNFYFPGYAEGGMSGDLTGSANATNNYQRMPHILTMSTVKQAIPRMWLPEAITR
                     GKAMGYLTAVDSLQGQFIPFGYPAPGHVPVQMLFRYGSASFGTMVDGNRWVNMYQHES
                     LKFVVNQSVWWEGETSYCDIILPACTVFERWDIGEWYNVGPAYVHHMYSMNNHRVISM
                     QHKCIEPLGESKSDYDIFLALAERLDLGAVYSEGGNSEYEWCKRAFESSDVAKNMSWR
                     QFLKKGYYVVPPEPEGSRAPVAMRWFYEGRKKDVPEPAPLPSDYLGNYGEGLQTPSGK
                     FEFVPTTLKRFEDPERPPTNRYMRTFENPQDNPELAKYPLRLLSPHSRYPFHVMGDDE
                     GCFLRDIREHRVKVGEHYYLVARISPEDASARGIRDDDLIRLWNDRGSVVCAAQVTSR
                     LRPGVISASTASAEYRPVGEPGKSTDLGGCVNMLNPSKSITPKSHGIRPNTTLIQVEK
                     WTGVDTWKPAEVA"
     gene            101416..102258
                     /locus_tag="PKN91_00490"
     CDS             101416..102258
                     /locus_tag="PKN91_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26573.1"
                     /translation="MAAKWNLIVDVERCNNCRGCFLAVKDEHTGNDFPGYAAAQPAMG
                     ANWLNIERKERGSYPIVEAHFMPVMCNHCDDAPCMKAATNGAVSKREDGIVIIDPEKS
                     KGQKQIVDACPYGAISWNEEKQIPQAWIFDAHLLDQGWSKTRAEQSCPTDVFKSVKVE
                     DAQMQRIKEQEGLEVLHPEFGTKPRVYYKNLHLMTTCLVGGSVAAEINGVDECAADAE
                     VVLKKDGREIARTTTDTFGEWKIDKLAPNSGRYQLEITGVSGRFSMEFDLGAESLYLG
                     VMRL"
     gene            102466..103515
                     /locus_tag="PKN91_00495"
     CDS             102466..103515
                     /locus_tag="PKN91_00495"
                     /inference="COORDINATES: protein motif:HMM:NF020468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1838 family protein"
                     /protein_id="HNS26574.1"
                     /translation="MPKFLISRRESLGLAASVAGAAAASALGAASAAAAPAAAKKPNV
                     DFVTKVDFNSPEWNRDIRARMTSDVDPTKEKLGWLKGKVYGVRPNEAVRTLFAFEGFS
                     LVRTKRLPDGSWRRLLRETVFYRDIKTGKILKTWHNPYTNETVRVVPIANNPYNSTVT
                     AYSRSGKPYLGEWIEGPNNTLTLYSGVNLFYPNLLQPSKWPRESAGEFNQVTENFYYT
                     VNRADAENPNLTNVPVLGAWQRITPWLPWMLMGAAPGHIDYFSMFATIPGGRDGLKNL
                     PQDLVEAARENDPLMLSAPTEDNLPNESSLEAYAKEQQPAPVPPGWQPPQPPPPPEFT
                     GDPANLGRQGAGPSN"
     gene            complement(103719..104714)
                     /locus_tag="PKN91_00500"
     CDS             complement(103719..104714)
                     /locus_tag="PKN91_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNS26575.1"
                     /translation="MRVSLKAGGLVALACVWLGLVGCDGFREVPRAELEARYAVPTSK
                     FTEVDGTRIHYTDAGAGPVVVLLPPSFMNLRVWDATAEILAREFRVIRIDFPVIGLSG
                     PDPSQDFTMDRFADLLHGLTTTLGVEKFSLVGTSSGGIVAFQYAGRWPERVERLVLIN
                     CAGLPRTAATNPNRPRGGALSRWWRSHYSSRKARQDSMNSQMGTGRPAPAELVTIVHD
                     LNRREGIKEDSAVMLKQFRTGDPQAALAEVRAPTMIIWGKKNITLSHLEANVFELWLS
                     GAPSMVLKYDELGHYPYVEDPQRVAADLSAFFKGERDDELARTIRQPVAESNVVR"
     gene            104827..105342
                     /locus_tag="PKN91_00505"
     CDS             104827..105342
                     /locus_tag="PKN91_00505"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNS26576.1"
                     /translation="MSAAQHVLLERLFEERRHSLAEFFRRRLRATADAADLAQEVYLR
                     MLRLPATSHIDNPEAYLFTVAANLAREYAVLERRRGIRVRTEDCEGTRHMVQQPTFEN
                     DVDTSARVERLQEVLAQLSPKCRAAVLLQYKHGLSYDEIATQLGVSRNMVKKYLSQGL
                     AHCRRRMSRLA"
     gene            105370..106464
                     /locus_tag="PKN91_00510"
     CDS             105370..106464
                     /locus_tag="PKN91_00510"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNS26577.1"
                     /translation="MDAADMEIKGQASADISQQAAAWFVANQNPDEARARAQEFLAWL
                     RRSPEHVQEYLAVAQLARALGDCAEAPPIEQLVREAGTATENVVPLHGFRPPSAAPQR
                     GQWSRRRALPALAASFIVAIIGLAVWFSSAIPRPQASVAQYITRHGEQRSLKLPDGSM
                     IHLNSDSEVKVGFDTTQRRVELLAGQALFDVEKDPGRPFKVIAGRTEVVAVGTQFEVY
                     RRGDATLVTVVEGEVAVSANPLAGGASESPGKVALVAGQRLRVPQVVDAAASESIEVP
                     EIVNVRETIAWPRLQIVVDSRPLGEVVQEFNRHATPTIVIDDPGLAGRRISGVFDVHD
                     GDNFLEFIRRMDHVVVATYEDSVRLILDNN"
     gene            106554..>108970
                     /locus_tag="PKN91_00515"
     CDS             106554..>108970
                     /locus_tag="PKN91_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS26578.1"
                     /translation="MSRFSPLRTLLIAALFAAGAPGLALAQAPATLRATIPAQSLASA
                     LNVLATENDLQLAYESEIVAGRDAPAVPVGTPLEQALRSLLGDSGLGYEFIGERSIRI
                     FKAAGATNTTAGSTRVAAAAGAARDSGLLEEIVVSARKRDEALLDTPLSVQAFTADDI
                     ESAGLRNLEDVSAMTAGMNFQKLGNSQSGRYNSAVRFRGLELLVTTPTSQTGSLFIDG
                     VNILGGAASMQFTDIERIEVIRGPQAAYFGRGTFGGAINYVTADPASSFRGRASLEYS
                     PNFGSSGISASIEGPLGDTLSGRLSVASNKRGAMFTATDGGALGAERTDMITGTLLWQ
                     PSDALRVRVRALYGEDDDGPASSTYVPFRLHGNCPIGTPITVKTTAGEVDATMRRNLH
                     CGEVPVIPVTNNTTFYTVDTSRGTFNTRDILVDNYGLPATDTPRMDHFGLRSIFHLYT
                     GSIDYEFNDALTMSVLLGYNKRDTTQIRDDDNYDTFGRVTKGYMSLESTSVEARLYYD
                     GGNRLRGMLGVNYFDQDTFGDVDGGIGLVTNLLGVPSVAAGGGSVEAGKTDTSAVFAS
                     VEYDLLENLTLSLEGRYQDVSTDSLQGTYPGPYTESKTSRHDAVPRFLVTYKPFENTT
                     LYANYGEASLGGSRNASYAIRTPAEQEVIRGLVPGVSEQLAEQKLEAYEIGWKQSLGS
                     SRSWFALSAYTMDWINIPGSAQLTFISPTTGNPLSMGVTLPGNAELTGFEVELRWQPL
                     DQLVLNAAVGVVDAEYSSFISAGLNSYFTLPMGANYKTDGATLPRSPQETFALSATWT
                     DTLNSDW"
BASE COUNT        17593 a        36039 c        37182 g        18146 t
ORIGIN      
        1 gagaacttcg cactcggggg tagcgctccg gcggctgccg cgcccgctcc cgcgcccgct
       61 ggcgtgccac ccgcgataat cgcggcgccg gcgcccgccg cggccgatac cgctccgtcc
      121 gtcgccgccg cagcaccgcc tgacgcagcg cctctgtccg cgccggcgcc gagggtgtcc
      181 gcgcccgagc cgcgcatcga gtacggcgtg cagctcggcg cgttcagcag cgaggacaag
      241 gcgcgggcgg aatggcagcg tatcagcggc aagttcgcga atgtgctcgg tggcctttcg
      301 ccggctgttt ccccggtcaa gagcggcgcc ggatccctct accgtctgcg tgcctcgacg
      361 gcgagcgaat cggccgcgcg cggtatctgc gagtcgctgc gtgcacaggg ccagccctgc
      421 gtggtcgtcc tgcccgtcgg aaagtgacgc tgcatgtcac ctgccgccgc ggcgtttcca
      481 ttggtgcgaa cgtaagtcgc tgattcatca ggctacggcc gctggcacgt cgcttgctcc
      541 actccctgcg acatgagcag tcgcagctac acgctactga tgcccgcgat gtgcccgagc
      601 gacagcgtcc gacactggcc ggtcgccttg ctcctcgcca gtcgcggtgg cgctctgtcg
      661 cagactcgta tcgatgcgcg cctcgcccgc gcgcattgcg tggcgaaagc cctggccgcg
      721 gcaagtaccc ggtactccgt cggcacgagg gactgacccg cccgaacagg atcgcgaaca
      781 acttccccgg tgttcgctca actccgccgc ggcagccaga ctgtccgcgg cgtctttttt
      841 tccgcagcgc gcgcgggccg ctagaatgcg cccatgcatc gcctcatcct tcgcggcgca
      901 ctcatcgcgc tgctgctcgt caccacggcg cacgccgctg caccagcaga gatccgcacg
      961 accacccttg ccaacggtat gcgtgtgatc gtgtggccag accacgacat ccccaacgtc
     1021 gcgctctaca actacgtgcg cgtcggcagc cgcaacgagg cgcccggcat caccgggctc
     1081 gcgcatttct tcgagcacat gatgttcaac ggcaccacgc ggcgtccgcc cggcgagttc
     1141 gaccgggaaa tggaggcgcg cggcggccgc aacaacgcct cgacctcgga cgacctgacg
     1201 atctacatgg actggtttcc ggccggctcg atcgaggcgg tgctcgacct cgagggcgat
     1261 cgcctcgcca atctcgcgtt cgatccgcag gtgatcgaga gcgagcgcgg cgtcgtgtac
     1321 tccgagcgtc gcctgcgcgt cgaggacgac aacaacggtt tcctgttcga gcaggtgcag
     1381 agcacggcgt tcgcggcgca cccgtaccat ctcccgacca tcggctggcc ctccgacatc
     1441 gagggctgga ctctcgccga cctgcaggcg ttctatcgca cgtactacgc gccgaacaac
     1501 tgcacgctgg tggtggtcgg cgacgtgctg ccggacgccg tgtttgcgct ggcgcgccag
     1561 tacctcgagc cgattccgcg gcaggagccg ccacccgcgg tacgcacgct cgagccggtg
     1621 cagccgggcg agaagcacgt cgccgtcgag cgtcgcgcgc agaccccgct gctgcaactc
     1681 gcctacaagg cgccgcaggc cgacgatccg cgcaatccgg cgctgaacct gctgtcggtg
     1741 atcctgggcc ggggcgatgc ctcgcgactg catcgcctgc tcgtcgaaga ccgcaaggtc
     1801 gccatcagcg tcgataccta cctgcaggag ggcttcgatc cgggactctt ctgggtattc
     1861 gcgacgctgc cggccggcgg cgatccggcc ggagtgcagg cgcagatcga cgcggagttc
     1921 gcgcgtctga tcgaggatgg cgtcaccgac gccgagcttt cccgcgcgcg caacctgttg
     1981 agcgccgctt actggcgcca gctcgccacc atcgacggca aggcggcgct gctcggcgag
     2041 ttcgaagtga tgcacggcga ttaccgcaag ctgttcgacc tgcccgatgc cttcgcgcgc
     2101 gtcacggcgg aggacatccg cgcggttgcg aaggagctgc tcgatccgcg ccggcgcacc
     2161 gtcggtgtgc tcacgccgcc cgcgaacgcg ggggagggct gacatgcgca cgatcgcgat
     2221 tgccctctgc gccgcggtcg cgctgctcgc caccgccgcg cccgctcctg cggccggtgt
     2281 ggtggtgccg ccatatacgc gtacggtgct gccgaacggc gcgaccctgc tcgtaatgcc
     2341 gcgcgaggac gtgccgctga tcgcgtttac cgccatcgtc cgcggcggct cggtggccga
     2401 gaccggcgag caggccggtg tcgcctcgct caccgcgggc ctgctcgaga agggcgccgg
     2461 gtcgcgaaac gcctttgagt tcgccgacac cgtcgaaggc gcgggtggca gcttcagcgc
     2521 cgcggcggcg agcgaggcga tcgtcgtcag cggccagttc ctcgcgcagg accgcgagct
     2581 gatggtcgag ctgctggccg atgcgctgct gaggccgcat ttcgcggcgg cggaattcga
     2641 cagcctgcgc aaccgtcgca tcgagtcgat ccgcgcggcg aaggactcgg acccgtcggg
     2701 gctgatccgc agctacggcc gcgcgttcct gttcgcgaag cacccgtacg gcagggcggc
     2761 cagcggcagc gagcgctcgc tcgcctcgat cacccgcgat gatgtgctcg cctactatcg
     2821 cgcgaacttc ggtggcgatc ggctgacgtt gatcgtcgcc ggagacgtgg ataccgtcgc
     2881 gctgacgaaa tccgtcgagg cggcctttgc gggctgggcg cgcgccgcgg tgccgctgcc
     2941 ggcgctcgcc tcgccggtgc gcacgaccgg gcgccgcgtg ctgctcgtcg accagccggg
     3001 tgcggcgcag acctacttct ggttggcgaa cacgggcgtt gcgcggaatt tcgccgctcg
     3061 cgcgccgctc gaggtcgtca acacgctgta tggcgggcgc ttcacgtcga tcctgaatac
     3121 cgaactgcgg atcaagtcgg gtctgtcgta cggcgcgagc tccggattcg tgcgcggctc
     3181 cgtgccgggc gagttcgcga tcagctcctt cacccagacc gacacgaccg ccgcggccat
     3241 cgacctcgcg ctcgcgacgc tcgccggcct tcatgaacag ggtctcacgg acgacatgct
     3301 cgcttcctcg cgcacttacg tgctcggcca gttcccgttg cagctcgaga cggcctcgca
     3361 ctgggtcgcg gcgctggccg agctcgagct ctacggcctc gatcgctcct atatcgaggg
     3421 ctacggcggc gcggtcgaag gcgtggaccg gcaggcggcg cagcgggtga tcgaggaggc
     3481 attcccgcgg ccggagaatc ttgcgatcgt gatgatcggc gacgcggcga agatcagggc
     3541 ggtggcggcc aggtacggcc cggtgaccga gatgccgctc tcggcgccgg atttcgccgc
     3601 gccccccgcg ccgcgcccgg tccgctgacg ctcaatcgtc cggcagcggc gtgaactccg
     3661 tttcgtcgac gacgggcgcg aagcgaccgc gtgcccagtc ctgcttcgcc tgctcgatgc
     3721 gctcgcggct cgaggccacg aagttccacc agatgtggcg cggcgtcgcg aagggctcgc
     3781 cgccgagcag catcacgcgc gagttgtgca cggcccgcag cgtgacggtc gcatcgcgcg
     3841 ccagcagcgc gaggcgcgcc gcgccgaccg cctcgccgtc gatgtcgact tcgccttcgc
     3901 tcacgtagat ggcgcgctcg gtgtaatcgg caggcatctc gagcaaggcg ccggcgggca
     3961 tcgacgcatc gacatagagc gtgggccaca gtacttcgac gggtgagcgg cgtccgtagg
     4021 cctcgccggc gatcacgcgc agttcgcaac cgtcgacctc gagatgcggc agcgtggcgg
     4081 ccgggtggtg cgcaaaggcc ggttcgatct cctcgtggcc gtcgggcagc gcgacccacg
     4141 actggatgcc gtgcatgtgc gcgccgcgct cgcgcagtcg tggcggagtg cgttccgaat
     4201 gcgcgatgcc gcgcccggca gtcatccagt tgacgtcgcc cggccggatc tccatcacgt
     4261 tgcccaggct gtcacggtga aggaaagcgc cggcatacag gtaggtcacg gtcgcgagtg
     4321 cgatatgcgg atggggccgc acgtcaatac cgcgaccggg cgggaagtcg gcggggccga
     4381 tgtgatcgaa gaagatgaag ggcccgacca tgcgtcgccc gggcgccggc agtatccggc
     4441 gcacgttgaa gccgccaagg ttccgctcgc gcgcgtcgat gaatctctcg atgggcatcg
     4501 gaccctcccc tcgacggccg atcatggacc atggatgatg gggaaggcca gtcagggagc
     4561 gatgccgtgc gcctgtttcc acgtgtagtc ggactcgtgt tgctgtcgtt cgtatccgcg
     4621 acgcaggtgc aggccgccga cccgcacgtg cccgcagcgc tgcaggactg gcgtggctgg
     4681 gtgctcacgg gcgaggaaca tcgcgcctgc gcccgcctcg cctcgccggg cgtcgcggcg
     4741 ggcagtcgcg aggcgaggag ctgcgtctgg ccggggcggc tcgtgctcga cgtgggcgct
     4801 cgcggcggcg agttccgcca ggcgtggcag gtcgaggccg acagctgggt ggccttgccg
     4861 ggcaatgccg accagtggcc gcgcgatgtc acgatcgacg ggcggcgcgg cgcgatcgtc
     4921 gcgcgcaacg gcattcccca tgcgcgactc gcgccgggcg agtaccagat cgccggtcgc
     4981 ttcgagtgga ccgcgcgccc cgaatccctc gcggtgccgg cagagaccgg cctcgtcgat
     5041 ctcgtggtcg acggcacgcg tgtcgccgcg cctacgcgca ccggcaacac gctgctgctc
     5101 ggtgcgcgcc agggcgtgca gcagtccgtc agcttcgatt tcgacgtgca tcggctgctc
     5161 gtcgatgaca ttccgctgcg tctcgtcaca cgcctgacac tgcgcgccac gggcggcagc
     5221 cgcgaggtgc tgctgggacc ggtgctgccg gccggcttcg cgccgatcgc gctcgactcg
     5281 ccgctgccgg cacgcgtcga agcggatggc cggctgcgcg tgcagttgcg tgcgggtgtc
     5341 ttcggcatcg atctcatcgc gcgcgccgtc gatggcgccg cgcaggtcac gcggccgtcg
     5401 gccgccgagc cctggcccgc ggaggagatc tggagctttg ccggcgacga tcgcctgcgt
     5461 gtcgcggcgc tcgagggcgg tgaatccatc gatcccgcgc aggcggatgt gccggccgag
     5521 tggcgcgcct atcctgcgat gcgggtgctg caggacacgg cactcggcgt cgtcgagcgc
     5581 agtcgtggca tggcgagcct cgacgacaac caactgcagg tgcatcgcca gctgtggctc
     5641 gatttcgatc acgcgggctt caccgcggtc gatcagctgc gcggcagcat gcgatcgggc
     5701 tggcggctcg acatggctgc gccgtggagt ctcggcagtg ctcgcctcga cgatgtaccc
     5761 ctgctggtca cgcgggggca gggcgaaggc acgaccggcg tcgagctgcg ttcgcccgag
     5821 cttgtgctgc agaccgtcgc gcgcagcgcc ggcgcgcgcg gtgcgctgcc ggcgaccggc
     5881 tggacgacgc gattcgaggc ggtcaatggc gaactacacg tgccgcccgg ccatcggctc
     5941 gtcgcagcgc tgggcgtcga ttcggcgccg gacgcctggc tcgaacggtg gggactttgg
     6001 agcctgttcg gcgtcgtgat catcgcggcg ttcgtcggct ggctcgcggg caagccggcc
     6061 ggcgtgctcg cgttcgcggc gctcgcgctc acttaccagg atgcgccgca ggtgatctgg
     6121 ctttggggca atctgctcgc tgccctcgcg ctggcgcgcg cggcgccgga agggcgcctg
     6181 cgcggtttcg ccctgcgcta ccgcacgatc agtttcgcgg tgctggccat cgtgctcgtg
     6241 ccgttcatgt ggagccagct gcgcctggcg ctgcatccgc agctcgacag cgcgtactcc
     6301 gccggctgga accttcccat gccgatgccg gcggcacgtg cgccggccgc gccaccgccg
     6361 cccgtcatgg acatggccac ggcgcgcccc gagatggctg ccgtgcagga agtgaagcaa
     6421 gagacgccgg aaccgcgcgc caggaaggac gtcatcgcga ccggcgccat ggcgtcgcgc
     6481 accgagcgct atgcgcccgg cactgtcgtg caggcgggtc cgggcattcc cacctggcag
     6541 taccgcagct acgcgtactc gtggtccgga ccggtcgagc cctcgcagac ggcgcgcttc
     6601 gtgatcgcag gcccgttcgt gctggccgcg tggcgcgtcg tgggtgtgct gctgctcgcg
     6661 gcgctgttcg cagtgttgct ggcgggttcg ggcccgaact ggcgagagcg gctgccgccg
     6721 cggtggcgac cctggctgac gcacgccgcg gcgcgcggga ccgcgccgct cgccgttgcc
     6781 ctgtgctttg gagtggcgct ggtggcgggt ccgtcgccgg ccgcagccca gctgcccgat
     6841 tcgcagctgc tcggtgagct gaaggctcgc ctgacgaaac ctgccgagtg cgagccaagc
     6901 tgtgccgagt tgctggcggc gcgcgtcgag gtcgaccccg cgcgcctcac gctcacgatc
     6961 gatgccgccg cgctcgcgca tctcgccgtg ccggtgcccg ctgcgcccgg cgcgtggctg
     7021 ctcgacagcg tgacagtcga tggcacgccg agtcacgccc tgcgtcgcgg cggcgacgag
     7081 catctgtggg taccgctgca gcctggtacg cgcacgatcg tcgtgtcggg ccgcatcgcg
     7141 tcgaccgatg cggtgcagct ggtgtttcca tggccaccga agcgcgtcgt ggtggcggcc
     7201 tcgggctggg aggtcgcggg tgtcagcgat gggcgccttc cgggtggctc gctcgatctg
     7261 tcccggcaca ccgaggcggg tgcacgaccc gttgcgggcg tcgacgtcgc ggcggagttc
     7321 ccgccgttcg cgcgcgtcgt gcgccgcttc gaaatcggcg cggagacccg cgtcgagacg
     7381 cgcgtcgagc gcgtcgcgcc gctgcgggcc gcgttctcgc tgcccgtgcc gctgctggtg
     7441 ggcgaatcgg tgctcaccga ggatgtcgag gtgcgcgacg ggcagaccgc gctcgcggtg
     7501 ttgccggccg gcgcagccgc cgtcggatgg gcttcgacgc tgccgcgcgc cgactccctc
     7561 acgctgactg cgcctgcggc cgattcgcgc ctggtcgagg tgtgggagtt tgacgtcgac
     7621 gcgcagtggc atgccacctt cgagggcttc gtcgccacgc tgccggagga actggggggc
     7681 gagtgggtgt tcgagttcca cccgcgcgcg ggtgaacagc tgacggcgaa gttcgagcgc
     7741 cccgttgccg ccgaaggtgc gccgctcgcg atcgaagcgg tcgagcgcac gctgtcgatc
     7801 ggcaggcgcg cgaccgattc gacgctggcc gtgcgctatc gcgcgaccca gggcgggcgg
     7861 cacacggtga agctcgcgcc ggaagcgcgc atcacgtcgg tgacggtcga cggcgaggcg
     7921 ctcgcgcttc gccccgagaa cggagagctg tccatcccga tcctgccggg cgagcacacg
     7981 gtgacggtgt ccagtacgca cgcgagcggg gtcgcgacga tcgtgaagcc ggagccgctc
     8041 gacctcggcg ctccggcgag caatgtgcgc accactctgt cgctgccagc gaatcgctgg
     8101 gcgttgtttg ccgcgggcga tggcgtgggg cccgcaatcc tctactggag cgagctcgtc
     8161 gccttcctgc tgactgcctg gctggtcggg cgcagtgggc gctccccgct gcgcacccac
     8221 gagtgggtgc tgctgggcct cggtctctcg accctgtcgt ggggcgtgct tgcgctcgtc
     8281 gcgatctggc tgttcgcgat gaagtggcgc gaaggctggg ccgggcaggt ggtgcggtgg
     8341 cagttcaaca ccgtgcaggt gctgctcgcg gtgctgactt actgggccgt cacggcgttg
     8401 ctcttctacg gcatccgcga tggcctgctg tcgacgccgg acatgggcgt gaccggcccg
     8461 ggttccggcg gcaaccggta ctcctggttt ctcgaccgaa cgggtggcgc gctgccccgg
     8521 cccatggtgc tgagcctgcc gctgtgggtg ttcaagtcac tcgtgttcgt gtgggcggtg
     8581 tgggccgcgt tcgcggtgct gcgctggctg cgcgcggcct ggaatgcctg gaagagcggc
     8641 ggctactgga ggtgacgggc cccgggtcgc gaccatcagc gaactcgcgc ggcgtttcgg
     8701 ctgcagtccc tcgacactga cggagctgct cgaacactac cggcaccacg cctactcgca
     8761 gaggctcgcg aggtagccct gcgcaatcgg cgagaggcgc ttgcgtgcca gcgcgcgcgc
     8821 cttcggcgca tcgacgatgt actcgagcgg gccggagatc agcatgcggc gcacgcccgg
     8881 atcgcgggcc gagatgcggg ccatcttgtt gagcacggtg aagccgcgct cgatcttctc
     8941 gcggggcacg cggcgctgct cgatggatcg cgcgaggtac tgtccgaagc gcgcatagag
     9001 gaagtaatcg tcgtcgccga cctggaatcg cgtttccgcg aagaagtcgg ggaagttcct
     9061 ctccaggtac tggctgacct tgtgcaggcg gggaccaggt tggctggcag cggacgcttt
     9121 gcgcttgctc atcgcggcgc gaacgttacg cgcgcgcagg tcacgatgca accgcagttg
     9181 cgcgcgcccg tcagcgccgc aggatgcggt tctcgagcca gggcgacttg cgcgtgaccc
     9241 cgtccttgag cggcaacgca cccacctgcg cctgtgccat gccgctcggc ggcacgccgc
     9301 acaggcccgt ctgccggaag tgcagtgcgg tagagaggcg cgcttcggcg ggatcgccca
     9361 gcgcatgatt gaaatcgtcc gcgaccgagc agccgggcaa cggcacgccc acggtgccca
     9421 ccgtgttctc cggtgagaaa ccgtcggcgt agtcgccgaa gttcttcgca ttgacgccgc
     9481 ggaactggat cgagaaatac gtcgtgccgc agttgtccca gggatagaaa ccgtagggct
     9541 tgccgcaggt cgtcgagccg atctgcacga ccatgacatc gacgccgcgc aggctgttga
     9601 tgatcgcctc gctcgccgag caggtgccgg gaccggtgag tacgaagacg cgcggcagat
     9661 cgagggcggg cagcggagtg ccgcccgcga cgctgaagtc ctgcgcgacc gtatgaaagg
     9721 gcatcggcgc gatcgtccga ccggtcaccg ggtcacgcgt cggatgctgg tcgttgaact
     9781 gcagtagctc gaaggtgcgg ccctgcgtct gcgcgccgcc ggcgatcatg taggcgagct
     9841 cgctcgcgat atcgaggtag ccgccgccgt tgtagcgcac gtcgagcacg aggtcgacga
     9901 tgccctggtt gcgcagcgtc gtgatcgcat cgaccagggc cagctcggcg gtcgcgatgt
     9961 ggtcgttgaa cagcaggtag ccgaccagac cgttcggcgt tgcgatggtg cgcacgttct
    10021 gcacgggggc cgccgtcacg acggtcgagg tcatcgacac ggtgcgctgc ggtccgccac
    10081 cgatatcctg gaccgtgaaa ctgtgcgaga ggcccgtgtc ggaggggtag atcgcctcgt
    10141 tcagcgcgtc gatctcgctg tcgacattgg tgttcacgag gtcgatgcca tcgatcgcga
    10201 gtacctgcgc gccgcgcgcg aggcccgcca tggttgcagg cgaggtgccg tccggctcga
    10261 tgtaggcgac gaccagctgg cgcggcggag tggcgctcag cagcgcccat tgcaggccgt
    10321 atcccgcgga tacgcccgat tccgacagtt gctgccactc gctcgtcagg taggtgaagt
    10381 ggaactggtc cttggggcgg cccgagggcg tgagggcatc cgtcttgagc agatcgaagt
    10441 attcgagcac gccgtagccg gccgggttga gatcgggaac ctcgcggtac cagagataca
    10501 gctcatgggt ccacgagcgc agccagttct tctcgtcgag ggcggtgccg agccgatcgg
    10561 gccacgggtc tccactcacc ggatcattgc cgctgcgcgg gtttgcgcag cgggactcgt
    10621 aggtcgagga ggccggaaag aacccgggcg tgaagccggt gcccccaccg cccccaccgc
    10681 ctccaccacc accgccgcca ccgccgccga tcgagccgtc cccgccacct ccacaggcga
    10741 cgagcactcc cgcgagcagc aatgccgatc ctggatgctt gaaccccatg gtcattcctc
    10801 aggttttcag cgcatgccga cattgaccgc acggtcactg ccggtatgat ggaaaccata
    10861 gtagttcaag gtgttgcacg gcgtcgctcc cgggatatat ccctggcctc gggtccggtt
    10921 ggcggtccac gataaccttt tgaaaaattt accgttgcag gcattggcct gcggcgcggg
    10981 ggcggtgtgc ccgccgcgcc agtgttgatc cagaggccaa gaacatgacc gcatccggac
    11041 gcgggctata tcgtgacagc tacgagcgcg acagctgcgg ctttgggctg atcgccagtc
    11101 tcgacgacca gccaagccat tggctgatcg agacggcaat cctttccctc gcacgcctga
    11161 cccatcgcgg cgccatcgcc gccgacggca agacgggcga cggctgcggc ctgctgctca
    11221 agaaacccga gaaattcctg cgcaccgtcg cgaccgaggc gggcctcaag ctcagccgcc
    11281 gcttcgcggc gggcaatgtc ttcctggatc cccggccgga gaaggcgggc gcggcgagag
    11341 ccgagctcga ggcgcagctc acgcgcgaag gactcgaggt cgccggctgg cgggtgctgc
    11401 ccaccgactc gcgcgcctgc ggcgaggagg cgctgaagac gctgccgcgc atcgagcagc
    11461 tgttcgtcaa ctgccgcata cccgataccg acgaggcgtc gttcaaccgc cggctgttcc
    11521 tcgcgcggcg gcgtgccgag aaggcgctcg aggcatcgga tccggtgttc tatgtgccga
    11581 gcctctcggc ctcgacgctc gtctacaagg gcatggtgat gccccagcat ctcacggagt
    11641 tctatcccga cctgtccgat acccgactcg agtcgtcggt ggcggtcttc caccagcgct
    11701 tctcgaccaa cacgctgccg cagtggcggc tcgcgcatcc gttccggctg ctcgcgcaca
    11761 acggcgagat caacacgatc cagggcaacc gcaactgggc ggtggcacgc ggtccgctgt
    11821 ttcgctcgcc gctgctgccg agcctcgacg acatccagcc actcgtctcg ctcaccggct
    11881 cggactcgca gagcctcgac aacatgctcg aggtgttcct gatgggtggc ctcgacgtgc
    11941 tgcacgccat gcgcatgctg gtgccgccgg cgtggcagac gctcgattcg atcgactccg
    12001 acctgcgcgc gttctacgag tactacgcga cccacatgga gccatgggac ggccctgcgg
    12061 gtatcgtcac cactgacggg cggtatgccg cctgcctgct cgatcgcaac ggtctgcgcc
    12121 cggcgcgtta cgtgatcacg aagaaccgcc acctgaccgt cgcttccgag accggcgtgt
    12181 gggactaccg gccggcggac atcgtggcga agggcaaact cggcccgggc gacatgatcg
    12241 cgctcgacct gcacagcggc aagctgctgt cgacgcgcga catcgaccag atcctgaaga
    12301 cccgtcaccc gtacaagacc tggctcaagc ggggcgtgcg ttatctcgag accgacctga
    12361 tcgaccccag gctcgccgcc gagccgatgg accgcgacac actcggcctg taccagaaga
    12421 tgttcaacat cacggccgag gagcgcgacg aaatcatccg cgtgctcgcg gccgacgaga
    12481 gcgaggccgt cggttcgatg ggcgacgaca cacccatgcc ggtgctctcg caccaggtcc
    12541 gttcgctcta cgattatttc cgccagcagt tcgcgcaggt gacaaatcca ccgatcgatc
    12601 cgctgcgcga agcgatcgtg atgtcgctgc agacgcagat cgggccggag tgcaacatct
    12661 tcgcgccgtc gcccgaacat gcccggcaga tcgtgctcgg ctcgccgatc ctctcgcagc
    12721 gcaagctgcg gcagatcctt ggcctcaccg atttcggcgt cacgcaccag ttcatcgacc
    12781 tgcagtaccc gcaggaggaa tcgctcgagg ccgcgatcga acgcatctgc gacgaggcgg
    12841 agcgggcggt ccgcgacgac acgctggtgc tgctgctgtc cgaccgctac ctggtgcgcg
    12901 gcaagttgcc gatccacgcg ctgctcgcga ccggcgcggt gcaccaccgg ctcgtgcgca
    12961 cgggcctgcg ttcgaagtgc aatctgctgg tcgagaccgg cacggcacgc gatccgcatc
    13021 atttcgcctg cctgatcggc tatggcgcga ccgccgtgta tccgttcatg gcctaccaga
    13081 cgctgttcga catgatgcgc aagggtcgcc tgaagctcga cgccgaggaa cgcctcgaga
    13141 tcggcaggcg ctaccgcgcc ggcattcgca agggcctcta caaggtgatg tccaagatgg
    13201 gcatcaccac catcgcgagc tatcgcagct cgcagctgtt cgagatcgtc gggctcgcgg
    13261 acaaggtggt cgacctgtgt ttccgtggca ccgagagccg cgtgcagggc gccgacttcg
    13321 acgatctcgc gcgtgacgca gcccagctcg cggcgcgcgc gtggaatccg cgcgagccga
    13381 tcgcgcccgg cggcctgcac aagtacgtgc acggcggcga gtaccacatg tacaaccccg
    13441 acgtgatcgc gacgctgcag gcggccgtga tgacgggcga gtacgcgcgg taccgtgaat
    13501 tcgcgcgcct cgtgaacgag cggccggtct cggccttccg cgacctgctg cgcctcaagg
    13561 gcggcgcggc gccgatcgcg atcgacgccg tcgagccgat cgatacgatc atcaagcgct
    13621 tcgacagcgc cgggatgtcg ctcggcgcgc tctcgcccga agcgcacgag gcgctggcga
    13681 tcgcgatgaa ccggctcggc gcgcgctcga attcgggcga aggcggcgag gaccccgcgc
    13741 gctacggcag cgagaagaac tcgaagatca agcagatcgc ctcggggcgc ttcggcgtga
    13801 ctcccgccta tctcgtcagt gccgaggtgc tgcagatcaa ggttgcgcag ggcgccaagc
    13861 ccggcgaggg cggccagctg cccggccaca aggtcaacga gatgatcgcg catctgcgct
    13921 tcgcgcgccc cggcgtcggg ttgatctcgc cgccgccgca ccacgacatc tattcgatcg
    13981 aggacctcgc gcagctgatc ttcgacctga agcaggtcaa tcccgcagcg ctcgtgtccg
    14041 tgaagctcgt cgccgagccc ggggtcggca ccgtggcggc cggcgtcgcc aaggcctatg
    14101 ccgacctgat cacggtgtcc ggccacgacg gcggcaccgg cgcgagcccg atttcctcga
    14161 tccactacgc cggcacgccc tgggagctcg gcctcgccga gacgcaccag accttgcggc
    14221 agatggacct gcgccacaag gtgcgcctgc agaccgatgg cggcctgaag accgggctcg
    14281 atgtcgtgaa agccgcgatc atcggcgcgg agagcttcgg cttcggcacc gcccccatgg
    14341 tcgcgctcgg ctgcaagtac ctgcgcatct gtcacctgaa caactgcgcg acgggcgtcg
    14401 cgacccagca tcctgtgctg cgctcaaagc acttcatcgg cctgccggaa atggtcatga
    14461 actatttccg cttcgtcgcg caggaagtgc gcgagatcct ggccgggctc ggcgtcgcgc
    14521 ggctcgccga catcatcgga cgcaccgagc tgctcgaaat cctcgagggc gagaccgcaa
    14581 gacagcgcaa gctcgatctc gggccgatcc tctcgaccgg cggtctcgtg gcggaccggc
    14641 cgcagtactg catcgatccg tcgaaccggc cgttcgacaa gggcgagctt gccgagcgca
    14701 tggtcgccga catgcggggc gcgatcgagg cgcgcagtgg cggcgagtgg cattacagcg
    14761 tcagcaactt caaccgctcg atcggtgcgc gcgtctcggg cgagatcgcg cggcgctggg
    14821 gcaaccatgg catggcggaa catccgctga cggtgcgcct gaccggcaat gtcggccaga
    14881 gcttcggcgt ctggaatgcg ggcggcctgc acctgtatct cgagggcgat gcgaacgact
    14941 atgtcggcaa gggcatggcg ggaggacgga tcgtgctgcg ccatcccggc acggcgggct
    15001 tcgtccatcg cgacacgatc atcatgggca atacctgcct gtatggtgcg accggcggcg
    15061 aactgtttgc cgcgggtgtc gccggcgagc gcttcgcggt gcgcaacagc ggcgctgtgg
    15121 cggtcgtcga gggcacgggc gatcactgct gcgagtacat gacgggcggc gtcgtctgtg
    15181 tgctcggccg caccggcgtc aacttcggcg cgggcttcac cggcggcttc gcctacgtgc
    15241 tcgacctcga gcgcgatttc gtcgaccgct acaaccacga gctgatcgac atctcgcgcc
    15301 tgcaggtcga ggcgatgcag gcacacgtgc agcacctgcg cgcgctgatc gggcgccacg
    15361 tcgaggagac cggcagcgtg tggggcgagg agatgctgaa cgacttccgc tcgtatgtcg
    15421 gcaagttctg ggtcgtgaaa ccgaaggccg cgtccatcga cagcctgatc gagaacctga
    15481 ggcgcgcggc atgagcgaca aacacctgca attcctcgag atcccccgcc gcgatccgga
    15541 gaagctgccg gtcgaggagc gcgtcgcgaa atccgcggag atctaccggc agtacgacgc
    15601 cgaagcggcg tcgcagcagg cggggcgctg cctgtcctgc ggcaatccgt tctgcgagtg
    15661 gaagtgcccg gtgcacaact acatcccgaa ctggctcaag ctgatcgagg aaggcaatct
    15721 cttcgaggcc gcggaactct cgcaccgtac gaactcgctg cccgaagtct gcgggcgtat
    15781 ctgcccgcag gaccggctgt gcgagggtgc ctgcacgctg aacgacggcc tcggtgcggt
    15841 ctcgatcggc gcgatcgaga aatacatcac cgacgaggcg ctgaagcagg gctggaagcc
    15901 ggacatgtcg aacgtcacgc cgaccgggcg tcgcgtcggc atcgtgggcg caggtcccgc
    15961 gggactcgcc tgcgcggaca ttctcgcccg cagtggcgtg caaccgatcg tctacgaccg
    16021 ctaccggcgc atcggcgggc tgctgaccta cggcatcccg ccgttcaagc tcgacaagga
    16081 agtcgtcgag aagcgccgcg aaattctcga gggcatgggc gtcgagttcc gcctcggcgt
    16141 gcagatcggc cgcgatatcg ccttcgacgc cttcctcgcg gaccatgatg cggtgttcct
    16201 cggcatgggc acctacacgc acgtgaaggg cggctttccg ggcgaggagc tgcccggcgt
    16261 gtacgaggcg ctcccgtacc tgatctcgag catcaggcac gaactcggcg agagcgacgc
    16321 ggaggcattg atcgacctgc gcggcaggcg ggtcgtcgtg ctcggcggcg gcgacaccag
    16381 catggactgc aaccgcaccg cgatccgcca gggcgccacg tcggtcaagt gcacctaccg
    16441 ccgcgacgag gcgaacatgc cgggttcgcg ccgcgactac aagaacagca tcgaggaggg
    16501 cgtcgagttc ctcttcaacc gccagccgat cgagatcgtc ggcagcgatc gggtcgaagg
    16561 cgtgaagctg gtcgagacgc ggctcgggcc gccggatgcg cgcggtcgcc ggttgcccga
    16621 gaccgtgccg ggcagcgagg agatcattcc cgccgatgcg gtcgtgatcg cgttcggctt
    16681 ccagccgagc cccgcgccgt ggttcgcggc gcacgacatc gcgctgcatc cgaacggccg
    16741 cgtgcgcgtc tcggcgggca ggacgacgcc gttccagacg accaacccca aggtattcgc
    16801 ggggggtgat atggtgcgcg gcgccgatct cgtcgtgacc gcggtgttcg aggggcgcga
    16861 ggcggcgaag gggatactcg gctatctcgg catcgcctga gggtacgcgc ccgccaagca
    16921 gttcgaggcg cagcggggag tacggatcac gtccgggtcg tactggacgg cgttgctcgt
    16981 ggcggggcaa ccgtctccgc ggctggcggg cccgagttca tgccttgcag accaccagaa
    17041 cttcctcacc cagcatgtca gtgaccttca ctgccacggt agtcgtaccg tccggggcgt
    17101 ccagcttgta ctcgccgacc acgaaatcgg tcctgcgaat cggcacatca gcgtggacca
    17161 cgttgaacac cgcaccgtca tagccggtgt caatcatcac gctgtccacc atcgcacgcc
    17221 agtccgccac cttcgcctgc accacaccgg actgctgggc caggcgctcc aggatgcctg
    17281 gggagacaac atccttgatc tccacgtgca gcttgccacc cttgcgtttg actgcgacgc
    17341 gggcctgcgc cttctcgtgc tgcatgaacc caccatactt cgggtcgctg cgcagctcga
    17401 tgacctcgat gtggtgcggg tcggtcttct tcttgcgcag gcggttccaa tcggccagcc
    17461 agcggtcggc ggccagctcc cttccgaggc agacgacagt gatggcgcgg tcctcgtcag
    17521 ggcgcgcctc cagctcgttg cggatcgcct ccagatccat cggtgaaagc ggatggccga
    17581 aaggaatgat cttgaccagc cgcttgccca gcaggccgtc gaagaaggca tcgctgcgca
    17641 gccgctccac gcccaggtat tcgcaggcca ggttcaccgc ctcgttgtgc tggatggcaa
    17701 ggtcgtagtc gttgacgcgc cagtgggtga agccgagctg cgccggtgcc ggcgcatggc
    17761 taccgctctc gtccagcaac ttgccctgtg ccggcttccc gcccgctgtg agctgctctt
    17821 ccacgacccg ctgcaggcgc ttggcggtgg tctggatcgc gcccttgttg atgtcgcagc
    17881 cgatccagcg gcggccgagg cgctgcgcag aaaccgccgt tgttccggat ccaatgaatg
    17941 cgtccaggac aagctcgcca gggttggtgt agccgtggag cacaagatcc agcaaaccct
    18001 ccggcttctg agttggatag tcagtcttct gcttgtggtc cgcaggcggg atcaacggaa
    18061 tcccccaaac attgtcgagc gtcttggagt ccgagtcttg atagacaaga tttccctgct
    18121 cgtcctttaa gttctccaac acgccgtcga ctttgacacg cttcagctgc tttacgacat
    18181 tctcgcgcgg gctggcgagc ttatggagct tctgagcgga gccaagtccg tatacaagca
    18241 gggtgttagt ggcccgcggg aggatcccct ttcgatcatc atgcatcttg ttgtagaagt
    18301 accaatagac ctcgttctgg aagctctgcg agccaaacac ttcatccatc acgcagcgca
    18361 ggtgatgtga tttcttccag tcgcaatgga gaacgatgat tgactcttgg gccatcaatt
    18421 ccttgaggag caacagccgc tcatacatga actgcagata gttgtcgttg gcccagatat
    18481 cggtgtactg gatctgctcg cccagggtgt agccctcgcc ttcgagcttc accttgccct
    18541 tggggccacg cagttccacc ttgcgcacgt agtcggcgcc ggagtcgaac ggcgggtcaa
    18601 tgtagatcag cttgatctgc ccgcggaagc cgttggccag caggtgcgca agtacttcct
    18661 tgttgtcgcc gtggtagagc aggccgccgc ggcgcaggcc gtccggccac gtgtcccaca
    18721 gggtgtcatc cggcgccgcc ccgccatagg tctcgaccag ttgcgcgggg aacgcctgca
    18781 cggtggccgg cgcacgcttg cccacccagg acagcatcgg cctgccgccg gctttgccga
    18841 ctgcgaccgg cgccttcgcg ccccgcacgc gagccagcat gtcggtcgcc ggcatcgcgc
    18901 tggtcgccga ttccgctgca gccctcttcg tcgaagacgg ccttgtagcc ttgttgacag
    18961 ctttctttgc ggccctacgt gcggtcatgc cttggccccc agaaacgtgc ggaccggagc
    19021 gaagtcgttg ttcgccaggg tactggtgtc ggtaaagagc atttcgtagg cgatgcggtc
    19081 gggattgagc ctggcccact cgcgcacggc catcgccttg cggccgcttt cgccgtcggt
    19141 gtagtcggcg cgcaggcgct cctgcttgat ctccacaatc agcagcttgc cgctgccggg
    19201 tgcacccccg gcctgcggct tgcgccggat caggaagtcg ggcgagtacg gatgccagcg
    19261 cccgtcctcg cccttgtact cgaccacgaa atccgtcttg tcgcggccca atgcgccggt
    19321 gaagtacacg tcttcgatgt cgccggtgtc gagcctcagg gtgtccagca ccttggccag
    19381 gaaatccttc tccggcagcg aatcgaagtt gtaggggctg tagtggaagc cgaagttccc
    19441 accgtagtcg gcacgagcat cctccacgct ccataaccgg tcggccttgt ccttgcggta
    19501 ggtgatctcg gcggtgcgaa caggcttgcc atccgcgtcc tgctcctcgt cgaagccttc
    19561 ctgccgcacc agagcgagcg ccacctcgat ctcttcggac tcggtttcgt aatggcgcac
    19621 ctgcgcttcc agttgatccg ccagggcatc caggtggcga tgcggcacgt cgtcgtcacg
    19681 ataggtgctc cgcagcgcat ccaggacggc gaatagcggc agccggtaac gagccgccaa
    19741 gtcggcggct acggtacggg tgtccgcagc gtcgatggtc gcatccactg gcagtgcgtc
    19801 acccagctcg cgcatgacac gcgtgctgct gacctgggcg gccaactcgt agcgctgcac
    19861 agtcagcgcg ggatcgctgt cctgcggccg ctgcagctcc agtttggccg ggattctcgt
    19921 ctccacccgg cgcacgcgga cgatctgtcg cttgagcacc agcggcggaa tgttctcctt
    19981 gagcagcacg atcttcgcgt ggcgcaaatt cgcttgattg cggttcagtt cagccaggga
    20041 ttcaccgtag gtctcctgca gctgacgatc cagcacggtg tagttgtcct gcgacaggta
    20101 gatcttggcc ttgtgtgcgt tgccgggcac ctggcgcagg cagcgggtcg ccgcctgcag
    20161 cacgaaattg ttggacgact gcagattgcg ggcgagcgcg caggcgaaca ggctgggaca
    20221 gttccagccc tccgtgccgc ggttgacgag caggatgacg cggtggggag ccattggatc
    20281 gttgagccgg ttgaaggcgt ccacgtcggc ttgcggactg cgcgagtgat tggccaggca
    20341 ctcggtcggc gcgcggccat tggccgccag cgctgcctcc accacggggc gcatggcttc
    20401 caggtcatcg gtctgcggga agtagagggc cagcttggcc ctggcgccat tgggcagcgc
    20461 aacgtcgccg taatgctcga ggaaatcagc gaccacttcg gccacgtagg gcttcaaatc
    20521 atcgccaaaa tcgtaagcct cgatattgcc ggacaccggc ttgaggtagc cgtcctcgat
    20581 accctgcgac aggccatacc agtacaccac gtccagcagt ggctgcttgt tgtagtaggg
    20641 cgtgccggtc gtgttcacca cgcagacgac attggtctcg gcggcaaggt agtccacggt
    20701 cttgcgcacc ttcttcagct cggcatccat ggactggccg taggtgtgat gcgcctcgtc
    20761 cgagaagaca cccaggtgcg gcagctgggc cagccgctgc aggcggcggt tggccacatc
    20821 ctgcctgagc tggtcggcgt caccggcgcc ggcaaacagg ctgccgaggt cgcccttgcg
    20881 gatcgcggac ttctggatgc ggatcttctc ggtgttggtg acgacgatgt tgtagtgcga
    20941 gccagggagc acatcgatgt ccgggctgcc atcgcgggta aaggtcagct tcacgctggc
    21001 ggaaaacagc ttgtgcatgc gtggcggcaa gatgccatcg aaaggcatgg tcgccagctc
    21061 gcgcagtgac tcgatgatgg tcttgccggg ggcgaaaacc agcgcgttgt gcacgaactc
    21121 ctcgtccggg tgctcctgcg ccatggcgaa ctcggtggcg atgatcgcgc cgatcagcat
    21181 ggtcttgccg gcgcccatgg ccagggcgag gatgtagctg gggtagtgga gggtcagggt
    21241 ttcgcgcagg gattcgagct tgtgttgttt gacgaaggcg tcgtcactga ggacgtgctc
    21301 gatcaacgcg tcgaagccgt gttcctcggc gtattcgcgc acgacatcgc tggtcaggcc
    21361 gagcgtcttg cgcaattcga ccctggttgg cgcgagcttc tggtacaggt cgaagatgcc
    21421 gggagtatct tccaccagcc gcatgtacca gtaagtttcc agcgcccgca cctgcgggat
    21481 acgcagaaag ggcggctgcc cggcatcacg gcccgaaagc cagtccagta tttcggcgat
    21541 ggcggcgtac cgctcatgcg gatagccggc tgcgcgccaa cctgccagtt tctcagcgat
    21601 cagtcgtggc agttgcatgt acagttggct tccggctcaa gggcagatca gttccagctt
    21661 cggatagtag gtgcgataac gcttcgcgtc tcgcgtcagc agcgccatgc cctcgatggc
    21721 ggcgtgggcg ccgatgtaga agtccggcaa cggcgaggtg cgcgcgcccc tggtgcgtcg
    21781 gtactgcagg aaggctttcc cggccaggaa gccggcctcc cacggcagct ccagccgggt
    21841 gaaggcatca gacggcaggg catcgtccag ctcctcgatg cgttcgaacc ccaccgagat
    21901 ctccgcatag acgatgggat tgatgcacag ctctgcctcg gcagcgcagg catcgagctg
    21961 ggcggccgac cagccatgcc actgcgggtc gtcagtcagc acgtccagca ggacgttggc
    22021 gtcgaccagt acgcgggtca cggcatgcgc atcatgcgcc gcgggtcagc cgcatgattt
    22081 cgttggtgcc catgcgggcg gtcgcgcgcc cacgcaagcg ggcggccagg ctgccgcggc
    22141 gtgccttggg ggggtgggcg atggtggtct cggcctcgcg cagggctgct tcgcgcacga
    22201 aagtggctac cggcaggccg tgcagctcgg cggcacggcg caggcggttc ttgtccttga
    22261 cgctcaggcg caggtcgaga cggtcggcgg ttgcggtcat atcagcctcc ttgtacggta
    22321 gcataccgta catgctgtct gcccgccaga agggaggaag ccgcagcgga ccggctcgac
    22381 gatcgtatcg ccgctcacgc cgatgccgcc caccacttgc acgcagcgat cagcgatgcg
    22441 gaacagggct tccggcacgg cggccttcgc catcgaggat tctctgctgc cgcatgcgcc
    22501 cgcttccgca tcggtatccc atctcgattt ccggcgcacc gggcagcacc aggagtcgga
    22561 ggtgtgcgcc gttcaggcgc ccgcacagtc aatagagcaa tggcaatttg gtcgacatca
    22621 tgtaccggac tcccctgggg gtgcgtgaca caccggtcac gaacttcgca ggcttgacca
    22681 cgaggacatc gcaaggcaac gcgcgcagca cacgctccgc cgtgttgccg atgacgacgc
    22741 gcttcagcgc cgagcgtgac acggcgccca tgacgacgat tgcgctgccc agctcgcctg
    22801 cggtgcgcgg gatcgccttg accggcgacc cgacgacgag gtgcctccgg cgggccggaa
    22861 ttttcgtccc gcgcagcgcg cgatcaagag ccttgcgcgc cgccgattcc gcggcatcct
    22921 tgatgttggc aacctccctg gggccgacgc cgacttccgg gtgcacgccg accggtatcg
    22981 gcccataggc gtgcatggca tgcaatgatc cgcccagggc gctggacaat cgtgcggcga
    23041 ctttgaggat ctcggaatcc agcccggccg gttttgcgaa cgcatgcgtc ggatcgattg
    23101 cagccagcac gatgggacga ccccaggctt cgtcgttctt gaccagcaga accggaaacg
    23161 ggctggtgcg cagcaattcc cagtcggtga gatgcaacaa ccaaggcgcg agacgacgac
    23221 cggcatggag gtcagccacg atcagttccg ccttgatcct gcgggctcgc cgcacgatcg
    23281 cctcatgggc cggataatcc cagtccgcgg ccgtgcgcac cgtcagcttc ttgcgtcgca
    23341 gcgggccggc catggcttcg agctgttcca cggcgcgctt gcgcgccccg cgctcgaaat
    23401 ttgccagact gccggtctcg tacaggtgag tgtcggcgga tatggggatg gtcatggcgt
    23461 gaaagagctc gagttcggcg cccagcgcct gcgcgagcct cgcggctttc gcgacagccg
    23521 gcggtgtgct cgaggccggg tcttttatcg cgaccagaat ccgacgctga ggcttcatca
    23581 gtacctcgcc agtgatgagt tgcgcgaagc ctccgcaggc gcttgccgca attctgcggc
    23641 aacccctgtt tcggctgaac gcgcatcacg gatgttcgaa tggacaccat aacataaccg
    23701 ctgcgcgaga cgccgcgtac catcggcgtg cggccggacg cgctcttcgg tgtcgtcggt
    23761 cgcaaagaca gccagggaga acgcacatgg caaggactgc acccaaaaca aggatctgtc
    23821 tctggtacga ccgcgaagcc gaagaggcgg cgaatttcta cgcgggcttg tttcccgaca
    23881 gccgcgtcct cagcgtccag cgcgcacccg cggactaccc gagtggcaag gccggcgacg
    23941 tgctggtcgt cgagttcacc gtgcttggcg tcgagtgcgg ctggtgcaag gacaagtggg
    24001 gcgtgtcgtg gcagatcacg ccgctcgcgc tgaccgaggc gatggcctcc gcggatccgg
    24061 ccgtggcgaa gcgcgtgttc gaggcgatga tgaccatgcg gaaaatcgac atcgccgcga
    24121 tcgagaaggc gcggcgcggc tgactcgcac cgcgcgcccc gaccggcgcc cgctacgcca
    24181 gccgctgcag ggtctcgcgt ttgatcctct tcgcgagcga ccacttgtgc acttcggtag
    24241 ggccgtcgta gatgcggaac gcgcgcacct cgcggaacac ctgctcgacg atcgtgtctc
    24301 cgctcacgcc ggtgccgccc atcacctgca cgcagcgatc ggcgacgcgg aacagggctt
    24361 ccgataccgc gactttcgcc atcgaggatt cgctggtgcc tgccgcgccg ctgtcgagca
    24421 cgccggcgca ccagtcgatc atgagctccg cctgtttcag ctcgatgcgg ttctccgcca
    24481 gcatgaaacc cacgccctcg tggtcgatga gcggcttgcc gaaggccgtg cgctttacgg
    24541 cgtattcgct cgcgatctcc tgcgcgcgcg tacaggcgcc gagccagcgc atgcaatggg
    24601 tgagccgggc cggggcgagg cgcacctgcg cgtacttgaa gccttcgccg ctctggccca
    24661 gcatctggtc acagtgcacg cgcaggttgt cgatccgcac gatcgcgtgg ccgccgggca
    24721 tggagctgtc gatggtgtcg agcacgcgct cgatcgttac tgcgggatgc ggcagttcga
    24781 tgagcagcat gatcgcgccg ttcgccgtct tggccatcac gatgccgacg ccggcatcgt
    24841 cggcaccggt gatgaacgcc ttgcgtccgt tgacgaccca gtggtcgccg tcgaggaccg
    24901 cggtcgtttt catcatcgag ggatcggagc cggcgccctc gtcctcggcc ggctcggtca
    24961 tcaggaaggc cgaacgggtg cggccctcga tgagcggctg caggaagcgc cgctgctgct
    25021 cgggggaggc gacgcggccc agcaagtaca tattgccttc gtccggcgcg gccacgttga
    25081 gcgccaccgg gccgagcggc gagagcccgg ccgcgcgcag cacctgtgcg gtttcgcggt
    25141 gcgagagatg cgagccatcg gcgaggatgt gcggcgtcag cagaccggcg gcgcgcgcga
    25201 ggtcgcgcag ctcggtgacg agatccttcg caggaccgtg cggaccgcgg cgcaggtcgc
    25261 gctcgtacgg cgcgatcttc tcgcgcacga aagcctcgac gcgcctagcc agcggtatct
    25321 gttcggtcaa tgcatgtctc ccggtgagga cgaagatctt acccggcacg cgccaggtcg
    25381 ccccgcagcc accgcgcgcc gagcaggaag tgcacggcgg cccaggcgat gatcgcgtgg
    25441 gtgatgaatt gcccgccccg cgtgccgcgc tcgaggctct cgcggcacgc ggccaggagt
    25501 tccggcccgc tgtcttgcgg cgcccgtccg cccgggcagc tcgtgaggaa ctcgccgaag
    25561 ccgaacggcg cgaaggcttg cgcgctgaag aggtcgatga gccagcccgt gaacagcggg
    25621 ccgccgccga gtccgacgaa gttgatgacg aaaagcagca gcgccgtcgc ggtagcgcgc
    25681 atgcgcgcat cgacgaggtt gtgcatgacg ccgaacgtcg ggccgaggta catgtagtgc
    25741 aggaatgccg caacgagcag caggcccgcg gcgaccgtcc acgtgtcctg cacccaggcc
    25801 agcaggtaga gcggggtggc gagcagcagc gtcagaccgg gaaagagggc gtaccacttc
    25861 ggctcgcgct caccgatccg gtcgcagatg cgtccgccga gcagcgtgcc ggcgcccgcg
    25921 cccaggccgg cgatgagtcc gtagatcagc cccacctgcg catagtcgag cgcgaagctg
    25981 cgcaggaaat acggcgcaag gaaggtcgag ccgccgtacg ccgcgaagct gatcaaggtc
    26041 gcgccgcagg ccatgtggcg aaacgagcga cagccggcca gccgtcgcac gaccgcgccc
    26101 agcggcgccg gcggcgccgt gtccagcggt cgcgactcga tggggtcgca ctggccacgt
    26161 tgcggctcgc ggaccacgat ccaggtcacc acggcgagga cgatgccggg caggcccacg
    26221 atcaggaacg cctggcgcca gccgaacgtc tgggcgatgt agccacccgc caccgcgccg
    26281 atcatcgtgc cgaggggcac gccgaaggaa tagatcgaca acgccgtggc gcgccgctgt
    26341 gccgggaaat aatccgtgat cagcgaatgc gccgccgggg aacatcccgc ctcgccgatg
    26401 ccgacgccga tgcgatagag cagcagttgc gcgaagttct gcgccatgcc gcacagcgcc
    26461 gtgaatcccg accagacgaa caggcagatg gagatgatgc cgacgcgact gcgacgctcg
    26521 gcgaggcgcg cgagcggaat gccgagcaag gtatacagca cggcgaaggc gaagccctgc
    26581 agcaggccga gctgcgtgtc ggagatgccg agatcgtcct tgaccgcctg gccgagtgtg
    26641 ccgaggatcg tgcggtcgat gaagttcgac gtgtacacga gcagcagcac gccgagcacc
    26701 gtgcccgcgt aggcgcgcga ttgtagtcgc atcagcgcgg cctcatgcgg cttctccact
    26761 gtcgatcacc cgcattgtcg ggcgggatcc acggctgcaa aaaaacggcc ccgcgcgagg
    26821 cagggccgga gttgaatcaa cattcgaggt cggtcgctgt cagcttccac cgcgtccggg
    26881 tcgagacccg taacgatggg atcgcgtttt cacggacttt taacgattta cggctggaac
    26941 gttggggcgc tgccccggcc aactgctaaa ggcccgcgtg tcagttcacg cccttggtcg
    27001 ctcccgatag cggcaatatt taccgccgtc tggacccgtg cccgcgccga ctgcctcgac
    27061 tttttatcgg taaaccctgt gcgctcctcg catcacccgg atgctgtatc gccacgtaca
    27121 caaactgcaa ttgtcgcgcc agctccctga acccatgcca aatccgcgac ttgcattggc
    27181 aactagactc caatgggagt tatttgtaaa gcctgtcgac aaaaatcgac gtcgttttgg
    27241 cacgacacga gctggtcgtc agctcctctc gagctcgacc agctcgtacc aggtctgcat
    27301 gtagccggcg acgccgccct gcaggaacag gaaatccccg tcggacgtgc accagacgtc
    27361 gtacggcggg tgctcctcgg gcagctcgac gttggtcacg aactggtagc gccaggcatc
    27421 gaaggtgccg gcgcccacgg tgaccttctc ggggcccacg taaacgatgt tgacgccgat
    27481 cgagtgcagc atcgggccgg tggcgccgcg atggtcgggc gaggagagca gcatctgcgg
    27541 tatgcgctgc acctcgccgg gccgcttgcg gtcgatcagg cgcaggtgcc aggcgtcgca
    27601 ggcgatcgcg tggttctgga aggtgacgag cggcctggcg agcgccatcg tctggcgcac
    27661 ccggccctcg gcggcggtcc atgtctcgca ttcggtgaga tctggcgcga agcggaacca
    27721 cccggagccc gcgaagcggt cgccgaccga gaggcgcacg aagcagtcgg tgggcatcca
    27781 ctcggcatcg agcgcatagg tgatgtcgcg catgacgctc gggcgatcgt cgatctcgca
    27841 atgcgcggtg atcgtccggg cgccgctcgc gtgggtgtcg atgcggaagt actcgcgccc
    27901 gcgctcctgg ccgagacgct cgggcttgcg gctggtgtag aggatgcgac ccatcacgct
    27961 cctgtggtcg cgctgcaaaa tcgacttcgt catcgaacct ccaggatcgt gccgcggcgc
    28021 atccagctcc tgaacagcag gtgctgctgc aggccgcgcg ccgcattgaa gagcataaac
    28081 gcgaaccaaa ggccgtggtt gccgaagccg cgcgtgagcc accacgcggg aaggaacacg
    28141 acgcccacgg ccaccagcat cacgccgaac attgcgccgg gacgctgtgc accgatgaag
    28201 accccatcgt agaggaatgc ccaggcgcag acgagcggcg agagcaccat ccacggcaaa
    28261 tacgtatacg cggcctcgat tacgccgggg acgctcgtca cgacgcggat cgcaagcggg
    28321 ccggccgcgc cgtaggcgag cgcgatggca agcgcgatcg ccagcgtcca cgcgaggcat
    28381 tcgtccacga tgctgcgcag cgacgtgccg tcgcgcgcac cgatggcccg gccggtcagc
    28441 gactcgacgg cgttggcgta accgtcgagc aggtaggcca tcaggtactg caggttcacg
    28501 agtatcgcgt tcgccgcgag tatcgcgtcg gtctggcgcg cccccatgct ggtcaggaag
    28561 gcgaacgcga acatcagcag caaggtacgc aggaagagcg cgccgttcgc ggcgaacaac
    28621 tggcgcagcg agcgcaggtc gagaatgtcg gcgcgccgcc agtggccggg gcgcgcgccg
    28681 agctcgcgca cgacgaggcg caggccgaga gcggcggcca ggtattccgc gatcagcgac
    28741 gcccaggcga cgccggctac gccgtagccg agcccgggca cgaagagcag gtcgagcgcg
    28801 atgttgacga ggttggcact gagcaccatg acgagcggcg cgcgcgcatt gccgatgccg
    28861 acgaaccagc cgatcagcgc gaaattcgcg agcgcggccg gcgcgctcca gatgcggatc
    28921 gaataatagg tccatgcctg cgcgtcgacg gccgcgctcg gctcgatgag ccgcagcgcc
    28981 aacatgccga gcggcacctg cgcgatgagc agcagcgcgc cgaggtacag tgccgaaccc
    29041 acaccctgcg ccagcaccgt gcgcaggcgt gtcgcgttcc cggcgccggc cgcctgcgcg
    29101 gcgaggccgg tggtcgtcat gcgcaggaag ttcaggaccg tgaaggcgaa gctgaggatc
    29161 accgcgccga ccgcgacgcc gccgatatag ctcgcctcgc cgagatggcc gacgacggcg
    29221 gtgtccacga ggccgatgag cggggcggag agattcgcga gcaccatcgg gcccgcgatc
    29281 gcgaagattt cgcgacgatg gtcccctgcg agatcccgcc agtgcacgcg ctactgcgcg
    29341 agcctggccg agtcgacgcg cgcgcgcagg tagcgcatct gctccgcagt caggttgccg
    29401 gtgttgatct gcgcggaggc gcgctggccc gcggcgaccg ggccgcggat ctgcacggcc
    29461 gcgcccctct gcaggatgtt gccgttggcg tcgaccagca ccggcgtgac cacgatcgcg
    29521 gcgatcggca gcggcgtgcg attctcgatc accacgtaga cacggccgct ggcatcggcc
    29581 tgcacggccg cggcgacata gttgccgggg ttctgcggca ggtcgagtcg cacgagctcg
    29641 gcggtcgctt ccttgccgta gctgctgttc gacgcggccg cagccttgaa gtactgcagt
    29701 gcagtggccc ggtcgccgcg atcgcgcgcg atcgcaccca ggtagtacgc agccggcgcg
    29761 gtcggcagca gcttcgcgct ccgctcgaag gcgctcgccg cggtggcctt gtcgccgagc
    29821 tggtaggacg cgatgccggt accgaggtgc gagccgaagt agttcggcgc gagcgcctcg
    29881 gctttgcggt agtagccgag tgccgcctgc ggattcttgc gcgcgacggc gatgtcgccg
    29941 agcagctgct gggtgcggcc gtcctgcggc agcaggcgcg ccgcctcgag cgccagcgag
    30001 ttcgccctgt cgagctcgtt cttgcgcgcg gcggcgagtg cctcgtcgta tttgtcgaag
    30061 cccggtttca tgtcgagcag cggccggacg cgcgcggcgt aggtgtcgtc gccaatcacg
    30121 ccaccacgac ccagttccgt cgccgtcgcg cggttgcgat cgacgcgctc ctgcgagggc
    30181 gggtgcgagg cgaacaggcc gtcgagccag ctcttgttgc gggccttgtc gcccgcgagg
    30241 cgcacgaagg tttcctgcag cgtgaccgcg ccccacggat cgtagcccgc ggccttcatg
    30301 tacttcatgc cgtagaaatc gctctcgagc tcggcatcgc gaccgtactt catcgtgacc
    30361 atctgcaggc cgatcgaagc gccgccgagt gcgaggttgg cgaggcccgc gtccatgccg
    30421 ccgaccgcgg cgccgatctg cgcggccgcc atgcccgcct gcatcagggt gccgcgctcc
    30481 tgggccttgg caccgtggcg cgcggccgcg tgcacgattt cgtgggccag caccgccgcg
    30541 agctcagcct cgctggtcag ttcggtgagc aggccgcggt tgaccgcgat cttgccgccg
    30601 ggcagtgccc atgcgttagg cacggagttg ttgagcacga cgaattcgta gggcaggtgg
    30661 cggtcggaca ccgccgcaag cttctggccg acaccgctca cataggcggt gagttcgggc
    30721 agcacctcga ggtcgccgcc ctcgacctgg cgcatcggcg catactgctg ctggccgatc
    30781 gagacttcct gcgcctcgga gacgaactgg atttccttct tgccggtgac cggattgacg
    30841 ccgcacgcgg cgagcgctgc acatagcagc gcaatgacga gcgacttgta cacggcgatc
    30901 cctcccgcgt tgcgtctaga aggcgttgat tcccgtcagt tcgcgaccga tcgtcaactg
    30961 gtgcaccgtc tcggtgccct cataggtgat gacactttcg aggttcagca tgtgccggat
    31021 cggcacatac tcggcgctga tgcccgcgcc gccgagcatg tcacgcgcgt cgcgcgcgac
    31081 gtcgagggcc atgcggcaat tgttccactt cgcgagcgaa acctgcgagg gcaccatgtc
    31141 gcccttgtcc ttgagccgcc cgagctgcag agacagcagc tgcgcggtgg tgatccggcg
    31201 ggccatgtcg gcgagacgga tctggatcgc ctgcgtctgc gcgagcgggc ggccgaacag
    31261 tacgcgcgac ttcgtgtagt cgatcatctg cgcgaggcag gcctgcgccg cgccgatcac
    31321 gccccaggtg atgccgtagc gtgcctgcgt gaggcaggac aggggcccac gaaggccgac
    31381 gacgccgggc agcatctgct cctcgggcac gaccacattg tcgaagaaca gcgccgacgt
    31441 gaccgatgcg cgcagcgaga acttgttctc gatctccggt gccgtgaagc ccttggtgcc
    31501 cttctcgacg atgaagccgc ggatgccctc gtcggtcatc gcccaggcga tgcagaggtc
    31561 ggcgatcgcg ccgttggtga tccacatctt cgcgccgttg atgacccagt ccttgccgcg
    31621 cttcttcgcg tgggtcttca tgttcgacgg gtccgagccg ccatgcggct cggtcaggcc
    31681 gaagcagccg atgagctcgc ccttggccat gcgcggcagg tacttctgct tctgctcctc
    31741 gctgccgaag gcatggatcg ggtacatgca cagcgaggac tgcaccgaca cgaagctgcg
    31801 gatgccggaa tcgccccgct cgagctcctg gcagatgagg ccgtagctga ccgaattgag
    31861 gcccgcgcag ccgtagccgt cgatcgagga accgagcagg ccgagcgctg cgagttcggg
    31921 cacgagttcc ttcgggaagc ggtgctgttc gaagcactcg cgaatgaccg gcagcacctg
    31981 gtcatcgacg agccgcgcga ccgagtcctg caccatctgc tcgtcttcgg tgagcagggc
    32041 gcggacgccg taaaggtcca tcgggtcgag ggcaacgtgc ttcttgctgg cgggtgcggc
    32101 cactttgggg tctcctgggc ggggccggcg attatagcgg cgcggacagg ccccgacgcg
    32161 gtgaagacta cgtattttgc ccggcccgcg gccgcgggcc gtagctgagc ctgagggcgc
    32221 ggtagacgaa ggcgagcagc cacaacggtc cgatcagcag aaactgcaag tccttgaaga
    32281 atgacgggcg cctgccttcg caggcatggc cgatgaactg cccgatccag gccactacga
    32341 agatgcccgc gcaggtctgc cacagcggcc agggcagccg cagcaggccg tgcacgatcg
    32401 cgaacacggc cacgaagacg agcacgttgc cgagcgcgag tgccggcgag agcctgaagt
    32461 agtacgcgag acccggcagc gcggtgatcg tcgcccagtt gagccacggc gaaaccgcgc
    32521 ggaacgccgc aggcaccggc aacgaccaca gcaggccgat caccgacacg acgatcgccg
    32581 gcacgcagat ccagtgcagc aacttgttga ccgggttgcg gtggctctcc ccgtattcgt
    32641 cgagccactg agtgaccgtg cgcatcgtgt aaccttccgc ggaccgacag gagcgacgct
    32701 accttcgcat gcccatgtcc gcaagttccg cgcgaccccg ccagcgcctg ctcggctggc
    32761 tggcgacgcg cgagtggctc tccgatgcgc gcaagctcga atggtatccg ccgttctggc
    32821 tgatgcggat ccgcgtactc gaactcgcgc ccgactggcg cgtcgtgcgg atccggctgc
    32881 cgctcagttc gatttcgcgc aacccgggtg gcgtgatgtt cggcggcttc caggcgtcat
    32941 tggccgatcc gatccccgcg ctcgcgtgcg cgcggatctt cccgggatat tcggtctgga
    33001 cccggggcct ggcgatcgat ttcgatcgcg gcggcagcac cgatctcgaa ttccggttcg
    33061 aaatgccgct gtcgcaggaa gcagacatcc gcgccgaact cgccgcccgg gggcgtgcga
    33121 cgcccacgtt cgactgcggc tattacctga aggatggcac gcgctgcacg cgggtgcgca
    33181 atacggtagc catccgtcca cagggatatg atcgcgccac gaccccgccg gcttcaccgg
    33241 acagcgtcgg atagccggcg gatttagtca tcgttcagtt tcggcgcgct agcatcgcgc
    33301 ccatccgcag tctggaggtc tcgcatatgt cattcgtcac cgccacccgc atggcgctgc
    33361 tggccgcatt cgtcgccgtg gccacggccg gctgttatac gaccgatccg tacaccggcg
    33421 accgggaaat gaacaagacc tcgaagggct cgatcatcgg tgcagcctcg ggtgccgtga
    33481 tcggcctgat caccggcgac agttcacgcg agcgccgcga acgcgcgctg atcggcgccg
    33541 gtgtcggtgg tctggccggc gcgggcgtcg gcgtctacat ggaccgccag gaggcgaagc
    33601 tgcgcgagca gctgcgcggc accggcgtgt cggtcacgcg tgtgggcgac aacatcacgc
    33661 tcaacatgcc gggcaacatc accttccaga ccgacagcgc cgatctcaac gcatcattct
    33721 tcggcgtgct cgattcagtc gtgctggtcg cgaaggaata cgacaagacg atcatcgagg
    33781 ccgccggcca caccgacagc accggcagcg agcagtacaa ccagcagctc tcggagcgtc
    33841 gtgccggcac cgtcgccgcc tacctgacga atcgcggcat cctcgccgat cgcgtcatca
    33901 cggtgggtgc gggcgagatg cggccgatcg ccaccaacga cacgcccgag ggccgcacgc
    33961 agaaccgacg tgtggaattg accttcgccc cgatccgcgc gcagtagcgc ggatccgggc
    34021 gcgcgagctc gccgcagcca gcggcgctac cgatacctag cgcgctcagg ccgcggcggt
    34081 gagggcgcca cgcagcttct tcagcgcggc ggactcgatc tggcggatgc gctcggccga
    34141 cacgccatac tcgtcggcga gatcgtgcag cgtggccttc cgatcgtcga cccagcgccg
    34201 ctgcacgatg tcgcggctgc gcgcatcgag cgcctcgatg gcgtgcgcga ggtgctggct
    34261 cgtatccgcc tccgcttcgg cctcggccac cagctcggcc gggtcggcat cgggggccgg
    34321 caggtacgcg gccgggccat ggccctcgtc gtcgtcgtcg aggtcgggca caggatcgaa
    34381 agacaggtcg cgcgaggaga gccgctgctc catctcggtg acttcctccg ggcgcacgcc
    34441 caggtcgcgt gcgacggcct gcgtctcttc gtgggtcagc caggcgaggt tcttcttcat
    34501 gcgccgcagg ttgaaaaaca gtttgcgctg cgccttggtg gtggcgacct tcacgagtcg
    34561 ccagttgcgc agcacgtact cgtggatctc ggcgcggatc cagtgcaccg cgaaggacac
    34621 gagacgtacg cccatggccg ggtcgaagcg cttgacagcc ttcatcagcc cgacgttgcc
    34681 ttcctgcacg atgtcggcga gcggcagccc gtagccgcca tagcctcgcg ccacgtggac
    34741 gacgaagcgc aggtgcgaaa gcaccaggtc gcgtgcggct gcgaggtcac cctcctcatg
    34801 gaagcgggtg gccagttcct gctcgcgctc gcgcgtaagc accggaatcc gcgagatctg
    34861 gtcgagatag gcatcgaagc tgccgatggg gcccgacagc aggaagtcgt tgcgacgagc
    34921 gacaagctgc atggtgtcca tatcctcccg atactagcac tcgctacatt agagtgctaa
    34981 aggggtccgg gagttcccct gtgcatgccc cagattaggc attcaaatca atagcttacg
    35041 ctatttcgcg ggcatggccc ccacctgtgg tccctaggcc cgcggctcga tcaggcgcag
    35101 ctgtcgcgcc gcggccagcc aggctcccag ccagccgagc gccgcgccgc cgccgaccag
    35161 gcgcagcgta tcgaggcgac ttgcgcccag catctggtac cggctgccat aggcggccgc
    35221 gaggtcagcg acggccgggg cgagcacgga ggtggccgcg agcacgatgg cggccgcgag
    35281 caaggcaccg agcaggccgt agagcacgcc ggtgtagagg aacgggcggc gcacgaaagc
    35341 attgctgccg cccaccagtt tcgtcacctc gatttccgcg cgacgattct ggatctcgag
    35401 ccggatcgtg ttgccgacga cggccagcac gcctgcggcg agcaccagcg cggcgacgag
    35461 cagcgcacgg cgcaggacct cgaggatcgc gttgaagcgg ttcacccact cgacatccat
    35521 ctgcacgagc tcgacctcgg gccaggacgt caggtactcg cgcagcttcg cgagatccgc
    35581 gggcgagtcg gcgccggcct gcgggcgcac gccgagcacg tgcggcagcg ggttgtcctc
    35641 gatcgcatcg agcgcggcgc cgaagcccga gtactggcgg aattcggcaa gggccgcatc
    35701 ggccgggatc acggtggcgg tggcgacacc cgagcgcgcc ctggcgcgct cggcgagttg
    35761 ctgcaccttc tcgagcggcg tgccgatcgc gaaatagacc gacaggtcga ccgcattcgc
    35821 gaaatcgccg gtcgcggcgc gcgcattgtc gacgaacagc ttgaggccga gcggcagcgc
    35881 gagcgcgagc ccgatcaccc cgaccgtgaa cagtgtcgag agcggcgcgc gtgcgagacg
    35941 gcccgcggcg ccgagcgcgg cctgaatgtg gtgcgcagac caggtgaccg ggccgatcac
    36001 ggcgcggcgg ctcccacggt cggcacgagc acctcctcaa cgccgcgcac atggccgccc
    36061 tcgagcacga tctcgcgctt gccgaattcg cgcacgaggt gcatgtcatg agtggcgacc
    36121 atcactgtcg tgcccacttc ctggaaacgg cggaacacgt tcatgacctc gagcgcgagc
    36181 tcggggtcga gattgccggt gggctcgtcg gcgatcagca cggcgggcct ggaaatcacc
    36241 gcgcgggcga tgccgacgcg ctgctgttcg ccaatcgaca gctcgagcgg cagcaattgc
    36301 tccttgccga gcaggcccac ctggtcgagc gcggcgcgca cgcgcttgtc gagctcgcgc
    36361 agcggcgtgc cggcgaccac cagcggcagc gcgacattgt cgaacaccgg ccggtcgacg
    36421 agcagcttgt ggtcctggaa cacgacgccg atctgccgac ggaatgccgg gatcctgcgc
    36481 gccggcagcg ggccggtgct ctggccgttg accgtcacca cgccgcgcgt cgggcgctcg
    36541 agcagagcaa tgagcttcag cacggtgctc ttgcccgccc ccgagcggcc ggtgaggaac
    36601 accatttcgc cgcgcgccac ctcgaagctc acgccggcga tggcctcgcg cccgttcgga
    36661 tagcgcttgg ccacgcgctc gaagcggatc actcagcggc ctccctgccc tcgacgagcg
    36721 cggtggcgaa cgcacgtgcg tcgaacacgc cgaagtcctc ggcggactcg ccgatgccga
    36781 tgaagcggat cggcagccgc agttggcgcg cgatcgcgac tgcgatgcct cccttggccg
    36841 tgccgtcgag cttggtgagc gcgagcccgg tgacgccgac cgcctcgttg aactcgcgcg
    36901 cctgggcgag tgcgttctgt ccctggttgg cgtcgatcac gagcagcact tcgtgcggcg
    36961 cggacgggtc cgcgcgctgg atcacgcgct tgaccttctt cagctcgtcc atcaggtgag
    37021 actggctctg cagccgaccc gcggtatcgc agatcagtac ctgggtgcca cgcgcgcgcg
    37081 ccgactgcag cgcatcgaac acgacggcgg cgggatcggc tcccgcctgc tgcgccacca
    37141 tcgcggcgcc ggtgcggtcg gcccacacgc cgagctgctc gatggcggcc gcgcggaacg
    37201 tgtcgccggc ggcaagcagg acgctcttgc cctcatgctg caggcgttgc gcgagcttgc
    37261 cgatcgtcgt cgtcttgccc gagccgttga cgccgaccac cagcacgaca aatggtttgc
    37321 gtgaaccact gatatcgagc ggcttggcac acggttcgag gatctcgaag atcgcgttct
    37381 tcagcgcgtc gaccagcgcc tcgacatcat cgagttcctt gcgcgcggca cgttgcgcga
    37441 gcgcctcgag aatctgctcg gtcgcctcga cgccgacatc ggcggtcagc aggcgcgctt
    37501 cgagttcctc gagcgcgacc tcgtcgatct cgcgcccgcg gaacaggttc gcgagatcgt
    37561 aggaaagcca cgaattcggc ttgttgagct tggcgcgcag gcgcttgaaa aagccctcgc
    37621 gcgcgttgcc gtcgtcggat tcgattgttg ccatgcgcct cgtctcgttg ccggcattgt
    37681 accggcaacc tggccgggtt ggccgcgact agggctcgac taccgtggga tccgaaatcg
    37741 cgaccagcgt gccgtccgat accgcgaacg tgaccgcctg ggtctgcatc tcgatcgtgg
    37801 ctgccagact cgcggtggga tcgagcagtg aggcgtgcgt gcccgcggtc atgttcacgt
    37861 aggcgcgcga ggccggattg aggcccggag tgcccacttt cgcgaggcca ccgagatcaa
    37921 tcaggcgctg ggtagccgaa ttcgggacca ccgtatcacc gaggacctgc tgcagcagga
    37981 tgggcttggc ggctgcggcg gctgccatgt agttgaccgg atcacccgcc tcgatgacgg
    38041 tctgcgcatc gcggaagaac tggctgtaga gcgtggttcc ggggatgagg ccctgcgcct
    38101 gcaggccggc gttgatgcgc gggccgaacg tgggcgaatc ctgcagcagc tgtgcgatga
    38161 gcccgcccgg caccgacagc gtcgcggtct gcacgccggc cgacagcgaa atccacggca
    38221 ccgcgacgat gctgccgagg gacagaccga cgaagtggat gcgcgtcgcg tcgatgtcgg
    38281 acacgccatc gccggtcagg tcgagatcgc cgacgctgcg cgtcagcgtg atcaggtcgg
    38341 ccgccgcctg gcgcaggttg tcgcgcgcgg tcagcaggct cgtcaggttg atgaagctcg
    38401 cgcccgacga atcgatcacg ccgtcgggtc ccgcggcgcc cgttgcgttg ttcatcacgt
    38461 cgagattgaa ggtgcgctcg ttcggcgcct ggtagagcgg gctcgtcgtg tcggtgatgc
    38521 cgtgcagtgg ctggtcgatc gctacgacca cgaaaccctg gcccgcgaac gcatcggcga
    38581 ggcccagcat gttggtgcga tcgccggtga tgccgtgctg gaagatcacg accggccagc
    38641 cggcggccgg ctgcgtgcgg cccgaggccg cgtttggcgc ggtcgcgagc agcggaatcg
    38701 acagggtctc ggtcgcgacc ggcagcggat tgaagcgggt gaggtgtgtc gtgccgccga
    38761 gcggcgacgg attgccctgc catgagccgg tgagcggtgc gctcttcgag aggaaatagg
    38821 gaatctgcag gatgccggcg tagatgtcgg cgacgcccgg caatgcggga ttgatctggc
    38881 tcgtgtcgag tccggtcgcc tgcgcaacga tcggttgcgc ggtggcggtg gccgcgaccg
    38941 cggcgagcga atcgcgcgtc gagccggtcg aaaagctgaa agagagcacc acgctcgcgg
    39001 gattcacgcc cacggcgccg gcgatctgca ggtgggcacc ggtcagctgg cacacgccgt
    39061 tcaatgtggg attcgtgatg gacgggcagg tggcgccgcc ggcgagcgcg gtcttgatgg
    39121 tcgcgtagtc gtgatcgggc actgccgcgg cgccggccgc atcggtgatg gcatcggtca
    39181 gcagcacgag atagccgttg ttggtcgcgc ccgtgctcgg cacgagcggc atgagcgggc
    39241 ggatctcgac gatggtgttg ccgacggccg cgtctgccgc gagtcccacg gagtaatcga
    39301 cgccgggcac gagcacgccg gcgataccca cggtcgcctt ggtcgcattg tcgatattga
    39361 cgcgtattac gcgcaccgac gccggtgtca gcgatgcggc attgactggt cccgagaagc
    39421 gcgcgcggat cacggtgttc acgccgtatc cgtcgagcgc gttgagggcg gcctgcgcgg
    39481 gcatcagcga attcgctggc gagatgttca gcgtgccgtc cgtggagccg gcgaaataga
    39541 ggtcggtcgg ccagggcagc aggccctgcg cgacctggaa cagcggacgc aggccggtgg
    39601 gtgcgggagt gacggtcgag ccgccgggca ggctgccgct cgtcgtgtcg gtgggcccga
    39661 tatcgggacc gtcgataccc gagccgccgc tgcagccggc gagcagcagc agaatcgatg
    39721 tggtggccgc gagtgtcaag ctgccctgtt tcggacccat gatctttcct cgcgcggcga
    39781 gtactggtcg cgagcgacct agggtacgct tcccgcatcg tggccagcaa cagcaggcgg
    39841 agcgcgcgac agttgcgcat catcggaggc gcatggcgcg gccggaagtt ccgctttccg
    39901 cccgtggatt cgatccgccc gacccccgat cgcgtgcgcg agacattgtt caactggctg
    39961 atgggccgcg tcgatggcgc gcgcgcgctc gacctgtttg ccggcagcgg cgcgctcggc
    40021 ctcgaggcct tgtcgcgcgg cgccgcagag gcgattttcg tcgagcgcga tgcgcgtgtt
    40081 gcgcgccacc tcgagggcgt gctcgcggaa ctggacgcgc ggcatgcgcg tgtcgtgaac
    40141 gccgatgcgt tgcgtttcct gcgcggcccg gcgcggccgt tcgacctcgt gtttctcgac
    40201 ccgccgttcg acagtgagct ggcgggcctc gccgcaggcg cgctcgaagc cggcggctgg
    40261 ctcgcgcccg ccgcgctcat ctacctggaa ttgcccgtcg cggtgcccgt tccgccactg
    40321 cccggcacat ggcgaatcgt caggtccggg cgtgcgggcg aggtcgggta tcatctcgcc
    40381 gaacgcgccg cgaggggagc aggagcagca tgaggcgtca cgccgtctat ccgggcacat
    40441 tcgacccgat caccaatggc caccacgatc tcgtgcgtcg cgcagcgacc atcttctcgc
    40501 gcgtcgtcgt cgcgatctcg gagaacccgg gcaagcagcc gctgctctcg ctcgaccggc
    40561 gcctcgcgct tgcgcgcgaa gtgctcgtcg acctgcccca ggtcgaggtc atgtcctaca
    40621 agggcctcac cgtcgagttc gcgcgccagc agagcctcac cgtgatcgtg cgcggcctgc
    40681 gcgcggtgtc cgactttgaa ttcgagttcc agctcgcgaa catgagccgg cacctgtccg
    40741 acgacatcga gacggtgttc ctgacgccga aggaacagtt cacgttcatc tcctcgacgc
    40801 tggtgcgcga gatcgccgtg ctcggtggcg acgtcagcca gttcgtgcac ccgatcgtcg
    40861 aggccgagtt gcgcaagcac cggcgcgagc catcatgagg cgggcggtcg tcgcattttc
    40921 gctgctgctg gcgaccctgt tcgccacggc gctgccggcc gccgggcccg cacccggcaa
    40981 ggccgccatc gcgagcgcgc atccgctcgc caccgcggcc ggccatgaga tcctcgccaa
    41041 gggcggcaat gcattcgatg cggcggtggc cgtcagcgct gcgctcgcag tcgtcgagcc
    41101 gatcggttcc gggctcggcg gcggcggctt ctacctgctg catcgcgcga gcgacaaact
    41161 cgatgtgatg gtcgacgcgc gcgagaaggc acccgcggcg gcctcgcgtg acatgttcct
    41221 cgacgcggac ggcaatgtag tgcgggcgct ggcgcgcgac agcgcgttga gcgccggcat
    41281 atcgggtgag cccgccggca tggaactgct ggcgcagaag tacggccggc tgccgctcgc
    41341 ggtctcgctg gcgccggcga tccgtttcgc gaacgagggc tttccgctct atccgcgcct
    41401 gcaggccaac atccagcgca agcgcgacgt ttttgcgaca cagcccgaag tcgcggcggt
    41461 gtggctgcgc gacggcgaga gccctgcgct cggcacgatc atccgccagc ccgatctggc
    41521 gcgcacgctc gagcgcctgg cgcggcatgg cgtgcgcgat ttctacgaag gtgcgctggc
    41581 gcgcgaactg gtcgccggcg tgcgcaggct cggcggcaac tggacggccg cggacctggc
    41641 cgcctatcgc gcactcgagc gcgcgccgat cgtcggcgcg taccgcggcg cgcggatcgt
    41701 ctccgcatca ccgccggcat cgggcggcat cgtgctagtc gaggcgctca atatcctttc
    41761 gggctacgat ctcgcgcagg tcgatgccgc gacacgcaag catctgatca tcgaatcgat
    41821 gcgccgcgcg catcgcgatc gcgcgctgtg gctcggggac ccggacttcg ccagcatgcc
    41881 ggtcgcggaa ctgatccacc cctattacgc ggcaggcctg cgcgcctcgc tgcggctcga
    41941 cagggcaacg ccgagcgccg agctgcccgg cgtggagccc ccgccccagg ggacgcagac
    42001 gactcacttc tcgatactcg atgccgaggg caatcgggtc gccggcacga tcacgctcaa
    42061 cgccttcttc ggcgccggcc tcgtcgtgcc gggcaccggg gtcttcctca acaaccagat
    42121 ggacgatttc tcctcgaagc cgggcgtgcc gaacggcttc ggactggtcg gcgccagcgc
    42181 gaacgcgatc gcgcccggca agcgcatgct gtcttcttcc acacctacct tcgtcgaaag
    42241 tgacaggggc ctgatgatcg cgggcagccc tggcggcagt ttcatcaccg gtatggtgct
    42301 gctggccacg ctcgatttcc tcgacggcaa agacgcggtc gagatcgtcg gcgcgccgcg
    42361 catccatcac cagtacctgc ccgatgtcct gcagttcgag accgacgcgc tgaccgccgt
    42421 cgagcgcgca acgctcgcgc agcggggcca caacctgcgc gaagcgaacc ggcgctgggg
    42481 caacatgcag gtcgtcacct gggactacgc gagcggccgc gtcgaggccg catcggatcc
    42541 acgcggccat ggcgaaggac aggtctactg aggtgctcga gcgccggctc gacggcagct
    42601 ggctgccgac cgccgcattt gtcgctgcag aggaggtccc ggtcggagag ttgcgcgtgg
    42661 cacgcctcga cctcgctgcc gggcgctacc gcatcttcga tcgcgaggaa cagatcctcg
    42721 acagcggcga gtacagcatc gatgccgcgg tgctgccggt cgcgatggac atggtcggcg
    42781 tcgccggccc caatgccggc cgcaggctcg aagccatcat cgacatcgag ggcgacacac
    42841 tcaccgtctg ctacgacctc gacggcctcg cgcggcccga gtcgatgcgg cccgagaaag
    42901 accagttgct gctgcggatt tcttacgcgc gctgcctgca gcgcccttcc tgaccgccgg
    42961 tttccggggc gcgccgttgc ctgccgcgct cttgcgtttc acgatcctga taccgcgcgg
    43021 cttcgcgacc ggcgccggca gcttgccgag cttcgcgata tcgaccgcgc gccacaggta
    43081 ccaggcggcg gccgtgcggt gcggcgccca gcgctcgccg aacgcggcga gcgccctcgg
    43141 cgtcggcatg ccgcgcaggc cgtaggccag gcgaaagccg ttgcgtacgc cgaagtcgtc
    43201 gaccggcagc acgtcgggac gcccgagctg gaagatcagc agcatctcga ccgtccagcg
    43261 gccgatgccg cgcacctgtg tcagccgatc gatgatctgc gcgtcgctga gtgtcgcgag
    43321 cgctgcgccg tgcggcacga taccgttccc ggtcttcgcc gcgagatcct tcaggctcgc
    43381 gatcttcgcg aacgacagtc ccaccgcgcg cagttcgtga tcgggccgcg cgaggacgtc
    43441 gccgggttcg ggaaatgcgc cactgccgca gagcgcgacg aaccggccga ggatgcgctc
    43501 ggcggcgatg ccgttcagct gctggtgcgt gatcgcgcgc gcgagcgatt cgaacggcga
    43561 gcgcgcgcct tcgggcgcga ggcggtaggg ccccgctgcg cggatcagcg cggccatgac
    43621 cggatcacgg gcggcgaaat gcttcctcat cgctgacact ccgggcacca gtaggttgcg
    43681 cgctggccct gcacgcggcg gcggatcggg gtgcggcaca cgcggcaggg ctggccggtg
    43741 cgctcgtaca catacagttt ctgccggaag tagccgggcg caccatcggc accgacatag
    43801 tcgcgcagtg tggtgccgcc ggcccggacc gcgcccgcga gtacggtgcg gatcgcacgc
    43861 acgagccgtg ccacttcgcg cgccgtcagg tgacgcgcct ggcggccggg gcggatgcgc
    43921 gcgcgaaaca gcgcctcgct cgcatagatg ttgccgacgc cgacgacgag gcgcgcgttc
    43981 atgatcagcg acttgatcgc cacgcgccgc cggcgcgtca cgcgccacag gtaggcggca
    44041 tcgaactgcg cttcgagcgg ctcgggcgca agcgcggcga gcagcggatg gcgcgccgga
    44101 tcgtcctcgg tgtacaggag gctgccgaag cggcgcggat cgttgaagcg cagcgtggcg
    44161 cccgaatcga gcacgaggtc gaaatgatcg tgggcgatgc gtggcgtggc ggccggcagc
    44221 acacgcagat tgcccgacat gccgaggtgc agcagcagcg tgccgctttc gaggtcgagc
    44281 agcaggtact tggcgcgccg gcgggtgcgc agcacgcgct ggcccgccac gcgcgcgggc
    44341 agttgcgcgt cgaccggcca cctgaggcgc ggttcgtgta ccgcgagcgt gacgatgcgg
    44401 cgatcgagca cgtgcggctc gatgccccgg cgggtcgtct cgacttcggg cagttcgggc
    44461 atgtgcgcaa cgataaaaga aaacggccgg tggacctacg ccgcaccggc cgttctgtcg
    44521 cgggacccgt cgcttcgggc ctacttgatc ttggcttcct tgtacgacac gtgcttgcgc
    44581 acgaccgggt cgtacttctt cgtctccatc ttttccggat ggaggcgctt gttcttcatc
    44641 gtcacgtaga aatggccggt gccggcggac gagagcagct tgatcttttc acgcatcgca
    44701 gtgtgctcct cagaccttca cgcccttggt gcgcagatcg gcgacgacgg cgtcgatgcc
    44761 gttcttctcg atcgtgcgca ggccattcga cgacacgcgc aggctgatcc agcgcttttc
    44821 gtcttccagc cagaaccgct ggacgtgcag attcggcagg aagcggcggc gcttcttgtt
    44881 gttcgcgtgc gagacgcgat tgccgacagc cgggcgcttg ccggtgattt cgcagacgcg
    44941 ggacatgata agccctcgaa attcagtaac ttggaagctg gcgcgggaac agggccccgc
    45001 gaaaaagacg ggctttatac cagtctggcg cggccggggc aaccgcccgg cctatttgcg
    45061 cccgggcggg cgcggctggc ttcgcggacc caccgggggc agcacctcga ccgccagcgt
    45121 gaaggagcgc tcctcgccgg ggcgcatggc gcccaccccg atgacatgcc ggccgatctc
    45181 cttgccgtgc gcgtcgcgaa tggcgacggt gacgcagtac tcgcagggct cggccggatc
    45241 cgggccccgg atcgtgccgt cgatgcgcat cgccgtgcgg gtctcgccct ggcgctgcgc
    45301 ggcgacggga tcgaacctca ggtgatgccg gcagcccggg cagacgctcg cgctctccag
    45361 gatgaccgcc ttgcagtgcg ggcaagggcg ggtcctggca gtgagcggcg cacgagtgcc
    45421 gagcggcggt cgtgcgctca tgtgccgctg gcggtgcctc gtgcttcgcc gtggccccgg
    45481 gagtgcttct cgtcccagcc gaactcgacc tgcccgcgca tgcgcgagcc cgccgcgacg
    45541 gtgagggcgc cggccttgac gtcgccggtc atgacggcgg tttcggccag ttccacgcgg
    45601 cgcgcggact cgacattgcc ctgcagctca ccggcgatcg cgacgaggtt ggcgcgtacc
    45661 tggccgttga gttgcgcacc ggcgtcgatg gtcagatcgc cttcgatgtg cacgtcgccc
    45721 ttgaacttgc ccgagatgcg cacgttgccg gcgccctcga tcttgccctc gatgttgagg
    45781 ccgctcgaga tcaccgtctc cttcaattca acgcgttcct tggggcgccg cgtgtcatgg
    45841 gcggaattga gcggcgccac ggccgggcgc tcgacttcgc gcaccgggtc ctttgcggcc
    45901 gacgggatcg gggcagggct gctgccgggt gtggtcgtgt ctttccaaag agccatgtat
    45961 gcctcccaac tagcaaagcg aacggccaca ttaaccgctg cggggactct taccaatgtc
    46021 ggggcatggc gacagggccg gggtctacag cagccccctt tcggcgaagg acatgcaggc
    46081 cccgtcgccg acgatgacgt ggtcgagcac ccggatgtcc atcagtgcca gggcatcgcg
    46141 cagacgccgg gtgatcaact cgtcggcctg gctcgcctcg gcgacgccgg acgggtggtt
    46201 gtgggccagg atcaccgccg cggcattgcg ggccagcgcc tggcgcacga cttcgcgggg
    46261 atggacgctc gccccgtcga tcgtgccgcg aaacagctcg tcgagtgcga tcagccggtg
    46321 gcggctgtcg aggtgcaggc agcagaagac ctcatagggg cggtctcgca gctgggcgac
    46381 gaggaattcc gcggtatgac gcggggcgtc gagcaccggc cgcgcctgca gggtctcctg
    46441 gtagtggcgc cgcgcgactt cgagggcggc ctgcagacgg caccagcgcg cgaggcccag
    46501 cccccgcagg cggcagacgg tttcgcgatc gctccccagc aggccgcgca ggctcccggt
    46561 gcagagcagc aactcgcgcg cgagcgccac ggcgtcgagc ccggcagagc cactgccgat
    46621 cagcacggcg agcagctcgg catcggacag ctgccgcacg cccagattca cgagtttctc
    46681 gcgcggctgt tcgcccgcgg gccaggcgcg aatgcccatg acgacctccc tgcaatgggc
    46741 ccatttggcc gcggacaggg gtgggtggcg agccgagaat cgggtctgtt tgcgcgcccg
    46801 gcgaaacggg cccgataatg cccggatgca gggccgcaag attctccttg gtgtgaccgg
    46861 cgggatcgcc gcgtacaaga gtcccgacct cgtgcggcgc ctcgccgaac ggggcgcgca
    46921 ggtgcaggtg gtgatgaccg ccggcgccgc gcagttcgtg accgccacga ccttccaggc
    46981 cgtatccggg cgcgctgtgc gcgacgacct gtgggacccg gcggccgagg ccgcgatggg
    47041 ccatatcgag ctcgcccgct gggccgacct cgtgctgatc gcgccagcca ccgcacacag
    47101 cctcgcgggg ctcgcacagg gccacgccga tgatctgctc ggcaccctgt gcctcgcgac
    47161 cgaggcgccg atcgccgtgg cgccggcaat gaatcgcgtc atgtgggcca atcccgcgac
    47221 ccaggccaac gtggcggtgc tgcgcgcgcg cggcgtgcac gtgtggggcc cgggctcggg
    47281 cgaccaggcc tgcggcgaaa cgggcgacgg gcgcatgctc gagccgctgg aactcgccgg
    47341 cctcgccgaa gcgctgctcg gcgcgccgcg cgggccgctc gccggcaggc gcgtgctggt
    47401 caccgcgggc ccgacgcgcg aacggatcga tccggtgcga ttcatcagca accgcagctc
    47461 cggcaagatg ggcttcgcga tcgcccgcgc ggcgcgcgat ctcggcgccg aagtgctgct
    47521 ggtcaccgga ccggtcgcgc tgccgactcc cgcgggcgtg cggcgcatcg acatcgaatc
    47581 agccgaggac atgctcgcgg cggtcacgcg cgaggtcgcg accaccgaca tctacatctc
    47641 gaccgccgcg atcgccgact atcgacccgt gcgacccgcc gcgcagaaga tcaagaagaa
    47701 ggaggccgcg ctcgacctgt cgctcgagcg cacggtcgac gtactggccg cggtggcggc
    47761 gcgtgccgac cggccgttcg tcgtcggctt cgccgcggaa accgaatccg tcgagcagaa
    47821 tgcgcgcgag aaactgctgc gcaagaagct cgacctgatc gccgcgaacg aagtcggtga
    47881 cgacaaggtc ttcgacaagg acgacaacgc gctcgttgtg atctcgcgta ccgatcgcac
    47941 cgaactcggg ccggcggcca aggcgcagct cgcggatgca ctcgtggcgc tgatcgccga
    48001 acgctatgcg gcgcaccgcg ccgcacagga tgccgcgaca cacggcaaag cccgcgcgtg
    48061 agcggcgtcg ccgcgacagc acaccggccg ctcaaggtcc ggatcctcga tccgcgcatc
    48121 ggcggcgaat ggccgctgcc ggcttatgcg acgccggggg ccgcgggact cgacctgcgt
    48181 gccgcgctcg acgcgccgct cgaactcgct cccggccgct cggaactgat tcccacgggt
    48241 ctcgcgatct ggatcgagga tcctgcgctc gccgccgtga tcctgccgcg ttcaggcctc
    48301 ggccataagc acggcgtcgt gctcggcaat ctcgtcggcc tcatcgattc ggattaccag
    48361 ggcccgttga tggtctcgtg ctggaaccgc ggcgcggcgg cctacaccgt gcagcccggc
    48421 gagcgcattg cgcagctggt ggtggtgccg gtggtgcagg tgcagttcga tgtcgtcgag
    48481 gatttcgccg cgaccgcgcg cggcgcgggc ggattcgggc atacgggacg ctgagtcggc
    48541 cggcgcgacg ccggcgccta gtgtttcgcg atgctcgact gaagttcgac gaacctgtcc
    48601 atgtccgcct ggaactggag cgcgacgctg tcgcgctcct gctgcttcgt cagcaggatg
    48661 ttgttctgcg cgcgcagttc gctgaccgtg cgcaccaggt cggcggcgag gtcgtcgggc
    48721 atgcgccgtg cattctcgtc gctgctgtag ggccggtaga gctgcgcctt cgcctgcagt
    48781 ccggtgaggc gcgtgttgag cgaatcgacg tactgcctgg ccgcggcgga ctggccgacg
    48841 atctgctcga ggcgctggtc acgcagcgcc tcgatgtcct tcaccgaggt ataggtagtc
    48901 agcaggaagc gatcgtgctg gcggcgcgcg tcgatgtcgc gatcgcgcgc ggcgtcgagg
    48961 gcgcgctgct cgggggtctt ttcggcctcg agccggcgga tcaccacgcc gtcctggttc
    49021 aggaccgcgc gctcgctctt ggcgtattcg ggcggaacat ggtcgccata atgcacgacg
    49081 cctttttcgt cggtccacct gtacgagact tcgcccgtgt ctgcgccctt gttcttgttc
    49141 ttggtcgcgg cgtcgaccgg gcctgcggca agtaccgcga gagtcagcgt gacggtcagg
    49201 gcgggccagc ggagcatggc ggtattctgc acgagcgggc ggcgcttagg ctatgccgta
    49261 acggtcccgg taggcctgca gcgcggcatg gtgctccgcg gaaatgcgac gcccgtcacc
    49321 cttcgcctcg ccgagtgcat cgatcagtcc gccgagggtc aggatgctga tgcacctgag
    49381 gccgtagtcg gcttccactt cctgcacggc cgaccgctcg ccctggccgc gctcctggcg
    49441 atcgagggcg agggcgatgc cgaccggctg cgcgccgctc gcgcggatca gcgccaccga
    49501 ctcgcgtacc gcggtgcccg cggtgatcac gtcgtcgatg atgaggaccc ggccctcgag
    49561 acggtggccg acgatgctgc cgccctcgcc gtggtccttc gcttccttgc gattgaacgc
    49621 ccacggcagg ttgcggccgt gctcgccggc gagcgcgatc gcggtcgccg cgaccagtgg
    49681 aatgcccttg taggccggcc cgaacagcat gtcgaacccg aggcccgcgc gggcaatggc
    49741 cgcggcgtag cagcggccga ggatcgcaag cgactcgcca tcgttgaaca ggcccgcgtt
    49801 gaagaaatac gggctctcgc gcccggactt cagcgtgaag cgtccgaagc gcagcgcatc
    49861 gcgcgccagc gcgaggtcga tgaatgtgcg ttgaaattcc tgcatgcggg ggtcctgtgt
    49921 acgcggcggg acgctatcat gcggcgatgc gcatcgtcac gctgaacgcg aacggcatcc
    49981 gctccgccgc ggcgaagggc gccttcgaat ggctgggccg ccaggattgc gacgtgatct
    50041 gcctgcagga gaccaaggcg caggagcagc agctcgcgga ccacccgatc gacatccccg
    50101 gttaccaccg ctatttccac gacgcgaagc gccccggcta ttcgggcgtg gcgctgtatg
    50161 cgcgccgcag gcccgatgcg gtctcgcgcg gcttcggcgt cagcgagttc gacgacgaag
    50221 gccgctatct cgaggcgcgc ttcggcaagt tgtcggtcgt gtcgctgtac ctgccgtcgg
    50281 gctcgtcggg tcccgaacgg caggcctcga agttccgctt cctcgatgct tttctgccgc
    50341 acctcgcggc gctgaagagg aaacgccgcc agttcgtcct gtgcggcgac tggaacatcg
    50401 cgcacaagcc gatcgacctg cgcaactgga aggcgaacca gaagaattca ggcttcctgc
    50461 ccgaggagcg cgcctggctc gaccgcgtat tcgacgaact cggctatgtc gatgcgttcc
    50521 gcgaggttga tccgcggccc gaccagtaca cgtggtggtc gaaccgcggc caggcccgcg
    50581 cgaagaacgt gggctggcgc atcgattacc agatcgcgag cccgggactc gcgggcagcg
    50641 cgcagcgcgc cgtgatccat accgccgacc ggttctccga tcacgcgccg ctcgtgatgg
    50701 actacgacct gaagcgcctg tgacggccgc aaccgccgcc acgcccgccg caccccggcc
    50761 gcgctttcgc gacgtgctgc gcgaaccgcg catgatcgcg gtgctcggac tcggggtcgc
    50821 ctccggcctg ccgtacaacc tgaccgacgg cacgcttcag gcctggctca aggattacgg
    50881 tatctcgaac acgcagatcg gcctgctgac gctcgtcggc ctgccctatg cgtggaagtt
    50941 cctgtgggcg ccgttcatgg atcgctacca gctgccattc ctcggccggc gccgcggctg
    51001 gatcttcctg ctgcagctcg cgctcgccgc ggtgatcgcg gcctgtgcgc tgttctcgcc
    51061 cgccgatgcg ctgaccgcgg tcgccatcct cgcgttcgcg atcggcgtgc tgtcggcctc
    51121 gcaggacatc gtcatcaacg cctataccgc cgacgtcgcc cagccgcatg agcgggggct
    51181 cgcggcgatg gcgacatcgg tgggctatcg ggcggcagcg tggttttcgt tttcgatcgc
    51241 gctgatcgtc gcgcagttcg ccggctggaa ggcggcctac ctgatgatcg cggcggcgat
    51301 gctggcgctc gcgatcggca cgctgttcgc gcccgagccg cgcgagcggg cggcgccgcc
    51361 cgattcgctg cgcgcctcga tcgtcgatcc gttgcggcag ttgctgtcgg ggccgggcgc
    51421 cgtcgcgcta gtcgtgctgc tcgtggtgta caaggtgggc gacgccttct cgctgcgcct
    51481 gttcacgccg ttcctgatgg acctcggctt ctccaaggcc gagatcggcg tggtcaccaa
    51541 gaccacgatg gcggtcgcga ccatcgccgg caccgtcgcc ggcgggctat ggatgatccg
    51601 gctcggactg tttcgcgcgc tgctgtggtt cggcgccttc caggccgtgt cgaacctcac
    51661 ctacatgatg ctggccgtgg ccggcaagga ccacgtgctg atgatcgtgg cgatcgcaat
    51721 cgacaacttc gccggcggca tgggatcggt cgcgctcacc gccttcatcc tcgcgctgtg
    51781 cgaccagcgc ttcagcgcct tccagtacgc actgctgtcg gcgatcgcgg tgattccgcg
    51841 aacctacctc ggcgtaccgg cgggctacct ggccgatcgc gccggctggc ccactttcta
    51901 tttcgtgtcg ttcatcgcgg cgatcccggg catcgcgctc gtgtggtacc tgcgccgcga
    51961 tgtccgcgcg ctcgatgcgc gtggtgcgcc gggcaccgtg gcgtgaccga tatcgacctg
    52021 tcgcgcgtgc tgtgcaccct cgacgatctg cccgtggaca gtgcgcgcga tttcgtcgtg
    52081 ggtgggggca gttggccgct gcgggggctc ctggtgcgca cgcgcgaggg cgtccacgcc
    52141 tacctcaatc gctgtccgca cgcgcgccat ccgctcaacc tgcggcccca cgagtttctg
    52201 accccggatg cgagtctgat cctgtgccgc tcgcacggcg cgctgttcga gcgcgcgacg
    52261 ggtcagtgtg tcgccggccc ctgtgcgggt caggcgctca ccgcgatcgc gatacgggtg
    52321 gaggcgggct gcgtactgct cgatgaggcg gtggacccgg cgtcattcga agatgtcgag
    52381 gccttctagc gggccggcga gcatgccgcg ggtctcctcg tagtcgtcgg agctgagggc
    52441 accgcgctcg aaatcgctgt cgacatcgaa cgaaccgctc cagtcctcgg gcggctcgac
    52501 ttcttcgagc cggcgctcct cggcatgcag ttcccagtct tcgatgtcga gttcctcgac
    52561 cgtgccgtcg aaatactgga tttctatcgt gccgtcgtcg gtatcgcagg cgaccacttc
    52621 aaaggcctcg ccagagtcga ggcgatacca ggcgccgata ttcgggaatg gagtcgccat
    52681 catcgtgcct ccgggccgaa ctgctatgct gatatccaaa gccatcctgc gcccgggaac
    52741 cggcgctttc aagcgggaaa tttgccgcgc agcatcatgt tcgatcaggt cgtgaaattc
    52801 ttcgtcgtat tcttcgtggt ggtcgagccc gtctcgctgc tgccgctgtt cgtgagcctc
    52861 accgacggcg cgaacgagga attccgccgg cgcatggcga ggagggccgt gctgctctcc
    52921 ggcgcgatcc tgttcctgtt tgcgctcttt ggtgcgaagt ttctcgaact gatgggcata
    52981 agcctcgaag ccttccgcat attcggcggt ctgctgctgt tcctgatcgc gctcgaaatg
    53041 gtgttcgcgc gcgaatcggg tacgcgcacg acgagccgcg agcaggccga gaaccgtcag
    53101 cgtgccgata tttcggtttt cccgctcgcg tttccgttca tcgccggtcc cggcgcgctc
    53161 gcgacgatcc tgctgtggtt cggtccggtg tcgatcaccg cgacgccagg gcactgggcg
    53221 gcgctgtttt cggccgtgct cgcggtgctc ctgatcgcct gggtgttcat gcggctcgca
    53281 gagcgcctga tgagagtgat cggcgtcact ggtgcgagcg tatcgagccg gttgctcggc
    53341 gtgatcctcg gcgcgctggc ggtgcagttc gtgatcgacg gactgaaagg cgcgttctag
    53401 gggcgcgccg cggcgcataa ggccgcgagc gcgctcaacc ggtccaggcg accgtgacgc
    53461 gaaagtcttc gagctcctgc tcgtagcgcg cacggtcttt ctcgagctcc ttgatctcgg
    53521 acttcaggcg gcccgcgcgt tcggcgagct gcctggtgtc gacgagcgcc tgcgggcgca
    53581 gctcggcggt actgtcatct gccacgacca gcgccgaccc tgaagccgtt cgacggctgg
    53641 cagtattcgc gcagtccctc gctgcggccg tcctggtatt gggcgaggtc cggcgtgacg
    53701 ccgtgtttcg cgcatgccct gcggtgctgc gcgacgcgat cacccgaatg gcccgcgacg
    53761 ccgtcctcgt agccgatggt gcgccagtcg acggtacgac attcgttgcc gctcatggac
    53821 gcgcagccgc cgagggcgag agcggcgaag gcgaggctcg tcgcgagggc gggcgcttac
    53881 gcgatgcgcg gtggcatagg ctgccaaggg tagcgtcgcg gcccgtgatc gcgcgtgagc
    53941 gccgtcacat cagcggcgtc gataggcgag gtcccacacg ccatggccca gccgttcgcc
    54001 gcggcgctcg aagcgcgtgg ccgcgcgctc cggatcgcgc ggcgcgaagc ccccgccggg
    54061 cacgaggttg tcgaactcgg ccgaggcatc cagcacctcg agcatctgca tcgcgtaggg
    54121 ctcccagtcg gtcgcgaggc ggaatacgcc gccgcacgcc agcacgcgcg cgaccgcggc
    54181 gacgaatggc ggctgcacga tgcggcgctt gtggtggcgc ttcttgtgcc agggatccgg
    54241 gaacagcagc agcacctcgt cgatgctcgc gggcgccagg cactgctcga ggacttcgat
    54301 ggcgtcgcga cagatggcgc gcacattgcg gctgccctgc gcctcgcagc tcagcagcag
    54361 gcggccgacc ccgggcctgt gaacctcgat gccgaggtag tcgcgttcgg gatggcgcgc
    54421 agcgagcgag gcgaggtgat cgccgttgcc gaagccgatc tcgagcgtgc gcggagcgtg
    54481 gcggccgaag atcgcgtcga gatcgagcgc tgtcgatccg tcggctgagg tgtcggtgtc
    54541 gatgccgtaa cgcggccaga ggttttcgag cgcgcgctgc tgcgcatcgg tcatgcggcc
    54601 gccgcggatc acgaagctgc gaatcgtgcg tttcatggcg cccgccccca gcgcgcgaga
    54661 tcccagaaca gcgcgtcgag gccgatgcgg atgccgggat cggcatccca gtcgtacctg
    54721 cggtcccagc gcacgagcgg ctcgacgaac cagtgcagcc agggattacc ggtcggtcgc
    54781 ttgatcagca ggccggtctc gtaatagtcg acgccgtgag cgtcgagtcc cgacgccagc
    54841 aggtgcaggg ccacgccctt gccgatgtcg cgcgcgcgca ggtaggagcc atagcgatcg
    54901 ggcacgatca actgttcggc gagggcgaag acgagtgact gggaccactc ggtctcgtcg
    54961 cggtcgttgc gccacttggc gtaggtcgag gagcgcagca ggcgccgccc ggaccagcgg
    55021 tcgaaggtcg ccgcggccgc gtagccgaca ctctcgtccg tctcggcgaa cagcttcgcg
    55081 aacgggtaga aatcccaccg gccgaggtgg tactcgcggc tccacttcac cgccatgaac
    55141 gcgaccggcg gcaggtccgc cttggcgccg atgtcgaagc tcacgtattt cagcagctgg
    55201 taacgcaggc cggcgcgcag gttggagtcc tcgccggcga cctccggcga ctcgtcgagc
    55261 tcgacgctcg tgacgaagat gcgcaggcgg tcctcgaggt tgggcagccg cagcgagaca
    55321 tcgaggtcga ggtcgagcga ggcttcggtc ttgtcgttgc ggccgagcag ctcgcccgtg
    55381 acgccaatgc gaaacggagc ggccggcacc ggcttcaact ccttgtccgg gcgcgcgtag
    55441 cgccggtcgg tcgcctcgac gaggcgctgg aagcgcgtgt aaagcacatc atgggcgcga
    55501 tcgagccgct ggcggaaatc gagcggcggg gcgatgtccc cggcactgat cgcctgcact
    55561 tcacccgtcg tgatctcggt cgggcggtag cgcgcgacag agtcgcgatc cgcgccgtca
    55621 ccggcctcgg cgagcacttc ggtggtcgtg atctcgcgcg gctgcgcgat gacgaccgga
    55681 tccgtcgggg cggtggtcac acaacccgca aggcatatca gcatgagcgg caggcaaggg
    55741 cgcagcagcg cggagcggct ggtcatgccg caagcatacg ggggcgcgcg ccggcgtgcc
    55801 gccgtcctgg gtcgcggaat gctccctcag tgcttgccgg gctcgagctt cacgccgcgg
    55861 cgctcccagg tcgcgtaggc gagcagcgcg accatcttcg ggtcatcggc ggcgagcgga
    55921 tcgccctcga gcggattcct caggcaccag ttgatcatct cccacaggcc cgcgacccgg
    55981 ccgagctgct tctggaactt cggataggtt tccgggtgcg tgttggcggc gttcgggtgg
    56041 cactggccac aggcgacgcc gttcttgccg agcttgccgt cgccccacag cgcgcggcct
    56101 tccgcgacga cgctctggaa ctgcgcttcc cagcgcgcca ggtcttcctt cgtgaattcg
    56161 tccgcgtgca gcgcgggcga ggcgatcgtg ccgaccgcga gagtcaatgc aactgtgatg
    56221 gctcgcatgg tggatctccc tcgggtaggc atcagaagtg gctctgcggc gcgatgcgcg
    56281 cggcggggtc ctggtaggcc gtatcttcgg ggcgcccgct cgccgcattg aacgcgacgg
    56341 tgcgcacctt gttgttgtag aggttgaagt gcgtcgccgc gcggcccgtg tggacgtcga
    56401 tgaactgcca gccggtcgcg tcgcgctcga agaacgggtc ggcgcggttc atgtgcacgg
    56461 tgagtttcgg caggtggctc tcggcctggg cgtaggtctc ggggtaaggc cacggccacg
    56521 cggtcgccat cgccgaggtg aacgcgatgt tgccgatctg gttgtactgg atctggtgca
    56581 cgtggccgta gaacaccgtg accttgtcga acggcgcgag gagttgctgc acctgctcgg
    56641 catcgtcggt ccagaagttc cagcccttgt agatcttctg cagcggcgag tgcgagaaca
    56701 ccaccaccgg cgtcgatttc gcgaccttcg cgaggtcctt cgccagccag gcgcgctgct
    56761 tatcgccgac catgaacggc gagccgttcg ggttgtcgag gccggccatt tcgagcatgc
    56821 gctgctcggc ggtcggccag cgctcgaagg tccactcgtc gtaggtgagg atgctgttca
    56881 gcacgacgaa gtgcacgccc ttgtggtcga agctgtacca ctgcgggccg aacagcttcg
    56941 accagtactc gccgaggtcg aggtagtagt cgtgctcgcc catcacgtga tggaccttgc
    57001 cgcgcagctt gccgagcatg tcggcgccgt ggtcgagctc gggcttgttg cccagctgcg
    57061 cgaggtcgcc gccgtacatc acgaagtcgg ggcgcggctg cagcaggttc gcctcggcga
    57121 ccgcgcggat caggccgcgg tcccagttgc gcacgaattt cgtgccctcg atctgctgga
    57181 tgtgcgaatc ggagatgtag gcgaacgtga agttctgctc cggtttcgcg aaggcgacct
    57241 cgacgagcga cagcggcagg gtcgcgaccg cgccggcggt gccggcgctc ttcaggaaac
    57301 tgcggcgtga ctggaggttc atggctgcgt tcctttggtc gtgccgcccg cggagggcgt
    57361 ggcggcgaac tcggggctcg tcagcgattc gaggaaggcg acgaggtcca ggatctcgga
    57421 ttccgtcagg tcgagcggcc ggatgccgcc cgagaggaag tcgttgaccg ggtcggtcgc
    57481 caccgtgacg ccgccgttgt tgtaatgatc gacgacgtcg cgcagtgtcg cgaggctgcc
    57541 gtcgtgcatg aacggcgccg tgatcgcgat attgcgcagg gtcgaagtct tgaacgcgcc
    57601 gagctcatcg agcgtgtcgg tgatcgcgaa gcggccgagt tccgagacct tcggatcggc
    57661 gagcaccacc ttgtcgacat cgatgccctc ggctttggcc cgcaggaacg ccggtgccag
    57721 cgtgccgatg tcgccctgga tgcggttcac gccgaccccg atgttgtgga agcggttgtc
    57781 ggtgaacagc gcatggtcct gctcgatcgt gtggcaggac acgcagcgac ccttgctcag
    57841 gaatacatcg aagccacgga tcgccgcggc ggagagcgcg ctcttgtcgc cgccgaagta
    57901 atagcggtcg aacggcgaat caccggaaac cagcgtgcgc tcgaatgcgg caatggcctg
    57961 cgtgacctcg cggatcgtga tcgcctcgcc gctcttgccg aacacggcgc tgaaggccgc
    58021 gacgtattcc ggatcggtgc gcacgatcgc gaggatggga tcgtggttcg gcaggcccat
    58081 ctcgattgga ttgacgaacg ggtgcagcgc ctgctcctcg agatcgggcg agcggccatc
    58141 ccagaagagg ctggtgaagt acgccgaatt cagcacggtc ggcgaattgc gcgtgccctt
    58201 cgccttgttg atgccctcgg cggtgaccag cggtccgtcg gcgaaaccct tctgcgagtc
    58261 gtggcagaag gagcaggcga cttcgccggt ggcgctgaag cgcttgtcgt tgaagagctt
    58321 gtcaccgagc gcgatttttt cggcggtctg cgggttgtcg gcgggaaccg ggaccggcgg
    58381 aaggccgagt gccgggccgg cggtaccggc agcggctgcg gtggccggga gggccgccca
    58441 tgccgcggcg gcgcacgccg cgacgaacca tagagtgcga ggcgcatgca tcgtcgttct
    58501 cccctgattc agcagcaagg ctgttttaga cttagtctat gggtcgagtc tagtccccgg
    58561 tttttggctg agtcaacgag cgcgcggccg gaatcggtga tcgaggccga tcagttggtg
    58621 agcagcgcga atgcgcggct gtaggcctgc gggtgcgcga gggcgacgaa cgcgagcccc
    58681 gtcagggcgc cggcgaagtg cgcgtcgtgg ttgatgcgcc ccctcgcgtg cttcgccgac
    58741 caccaggtgt aggcgaggta gcccaccgca aacagcggtg cggggatcgg gaacgggata
    58801 ggcagaatga agatgctctg tgtcggcagg tagacgatcg aggcgaacag taccgcgctg
    58861 atcgcaccgg acgcgccgag agtcgcgtag tcgggattgt cccggtgctt cgcccaggtg
    58921 ccgaactggc tcagcagcag gccggcgaaa tagagcacca ggaacgggcg cgtgccgatc
    58981 aggcgctcga gcggaatgcc gaagaaccag aacgtgatcg aattgaacag cagatgcggc
    59041 aggtcggcgt gcacgaagcc ggccagtacc gcggtcgggt agtcgcgctt gcgcaggaac
    59101 cagtagggac ggaagaggca ccgctcgagg aacgccggcg acccgtagag gccgaacagc
    59161 gagagcacga cgatcgtccc gagtatggcg atcccggcag gtccgatggc gccgctcatc
    59221 cccatgcgct cctcacgcga acggcgcgtc gatcgaggcc ggcggtgcga ccgtcgccag
    59281 ccgggcagcg tgcatcgagc cgaacgcaga cagtctcgca ccggctgcga acatccccaa
    59341 gcagttgtgt gagcctgtta ccatgagcgc catcatgaag gtacgcagaa gctacgtcga
    59401 tggtcgttat ggtcagatgc atgtgcgcag cgccggagat gccgggctcg cgcaccgacc
    59461 gctgctgtgc tttcacctga gcccggtgtc gggagccatc tacgacaagt ggctcgccga
    59521 gatgggctgc gaccggctcg cgctcgcgcc cgacacaccg ggctacggca tgagcgatcc
    59581 gccacccgcc gtgcccacga tcgccgatta cgccgcggca atgggcgatc tcgttgacgc
    59641 gctcgcgctc ggcgagttcg acgtcatggg ctaccacaca ggatccaaga tcgccgtcga
    59701 gctcgcgcgc cagcggcctg cgcaggtgaa gcacctggtg ttgatgtcga caccgatcta
    59761 cacgccggag gatttcgccg cgatccgcga gctgacctcg gtcgcgccga ttgccgccga
    59821 cgggcagttc ctggtcgaca ggtggaagaa gcatctgctc taccaggacc ggcgcgcgac
    59881 cctcgaaagc tcgatgcgca atttcccgtg gcacctgatg ggcggcgagc ggagcgactg
    59941 gggctatcgg gcggcgttcg gctattccta tccggacaac atcgtccagg tgaccgcgcc
    60001 gatcctcgtg ttcaatcccg aggacgacct gtgcacctac acgccgcgcg tgcgcccgta
    60061 tctgaagaac ggcatggtgc gcgacctgcc gggttggagc caccagctgc tggacttcag
    60121 caccggggaa atggcgacga tggtgcgcgg cttcctcgac gaggatcgct ttcccgccta
    60181 gcatgcgccc gtggaccaga tctcgcgcat taccatcacg atcgcgttca tcggcctcgc
    60241 cggcctgatc gtcggcttca cgacccgtga tcgcccgtac ggtacctatc tgatgtgggc
    60301 cggcgtcatg tgcatgatcg gcgtggtcgt gtaccacatc gtgaagaccg tgcagtcctg
    60361 acggcgaccg ccgccgtccg cgcgatcgcg cgcgctcaga tcacgccgaa cgcgagcatc
    60421 gcctcggcga cgcgccggaa gccgacgatg ttcgcaccga gcacgtagtt gcccgggctg
    60481 ccgtattccg ccgcggcctc gtggcagttg cggtggatgc cgctcatgat ctcctgcagg
    60541 cgctgctcgg tgtgctcgaa actccaggtg tcgcggctcg cgttctgctg catctcgagc
    60601 gccgaggtgg cgactccgcc cgcgttggcg gccttgcccg gtccgaaggc gatgccggct
    60661 tcctggaaca cgcgcaacgc gcccggcgtc gtcggcatgt tggcgccctc ggcgaccgcg
    60721 atgcagccgt tggcgacgag cttgcgcgcg gccgcctcgt cgagctcgtt ctgcgtcgcg
    60781 cagggcaggg cgatctcgca ggggatgtcc cagacgctgc cgccggcgat gtaccgcgcg
    60841 tcgcgatggt gctgcgcgta ctcggagatg cggcgccgct cgacttcctt cagccgcttg
    60901 accagatcga ggtcgatgcc gctcttgtgg tagatgacgc cgcccgaatc cgagcaggcg
    60961 atcacgctgc cgcgcaggcg ctgcagcttc tccatcgcgt agatcgcgac attgcccgag
    61021 cccgaaacga cgcaggtctg gccctcgatc gactggccgc ggaccttcag catctcgtcg
    61081 acgaagtaga ccgccccgta gccggtcgcc tcgcgccgca cgagcgaccc gccccagctc
    61141 gggctcttgc cggtcagcac cgccgattcg tagcggttgg tcatgcgctt gtactggccg
    61201 aacaggtagc cgatctcgcg cgccccgacg ccgatgtcgc cggccggcac gtcggtgtat
    61261 tcgccgaggt ggcggtgcag ctcggtcatg aggctctggc agaagcgcat cacctcgccg
    61321 tcgctgcggc ccttggggtc gaagtcggcg ccgcccttcg cgccgccgat cggcatgccg
    61381 gtgagagcgt tcttgaagac ctgctcgaag cccaggaact tgacgatgcc gaggtagacc
    61441 gacgggtgga agcgcaagcc gcccttgtag ggtccgagcg cgctgttgaa ctgcacgcga
    61501 aagccgcgat tgatctgcac ctcgccgcgg tcgtcctgcc acggcacgcg gaagatgatc
    61561 tggcgctcgg gttcgcagat gcgctcgatg atcttctgct cggcgtattc cgggtgcttg
    61621 gcgagcaccg gaccgaggga gtcgaccact tccttcaccg cctggtggaa ttcgacttcg
    61681 cccggattgc gccggatcac ctggtcatag atctgctgga ttcgcgcttc catgcgtcaa
    61741 tcatgcgcag taccgcaggc gatgtgaatg cgggaaacca cgcacgccgg cagggcgggc
    61801 ggtgcctacc agtcggccag gtcgcggctc ttgcgttgct cgcgcacgag cgtgatccgc
    61861 cacagctgct cgtcgcgcag ccagtgccag gtgcgtgcgc ggtgtatgcc gtcgcgcgag
    61921 agctggatca tctcggtgac ctggttgccc gggtccgcgg tgtacccgaa gcgcatatac
    61981 agggtctgcg gctcgattgc gcggatctcc ccgcgcaccc ggccgtcgtc gatcagcagc
    62041 ctgtccccct gcgcgatgcc ggtgtagacg agttcggtgg tgcggccgtc gtcccagcgg
    62101 tagcggctcg tctggcgata ggcaatcggc ccgtagtcgg gacactcgac cttgatgcga
    62161 aacgcgtagc tttcctgcac cgagcggtcc gccggatcga tgtgggtgta gtcgccctcc
    62221 cagatgccga catgctggcg cagcaggtcc attgttgcgt ttgcgccgct catgccgcgt
    62281 cagcccgggc cggctcgacg ctctcctggc atttgccgtg cgggcagatc gcttccatga
    62341 agtcggcatc gacactgagg cgctcgacga ggaagtcgac gaacgcgcgg accttgggcg
    62401 atttgccctg gccgcgcagg aacacggcgt tcatgtcgag ggtcgggcct tcccagcctg
    62461 cgagcacgcg caccacgcgg ccctgggcgg cgtccggctt caccatcacg tccgcaccga
    62521 gcatcaggcc ctcgcccgag agcagcgcgc cgcgcagcgc cgcgggatcg ttggagacca
    62581 gtaccgggtc gatggggaag tcggccttgt gcgcgccatt gctcaggcgc cacacatagt
    62641 cgttgccgct gttgcgatgg tagctgctca tcgcgaacgt gcgatggtgc accagctcat
    62701 cgggatggtg cggctcgccg tgctgcgcga tgtacgtcgg gttggcgtag acctgcgtgc
    62761 gaaacgaaat caggcggcgc gcgaccaggc tcgggtcgtt cagcctgcca atgcgcagcg
    62821 cgaggtcgaa ttccttgtcg atcaggtcga gggattcgtt cgtgagctcc atctcgacgc
    62881 gcacttccgg atagcgcagg tagaactccc cgagcagcgg cgcgatccag gtgacgccga
    62941 acgaaaaagg cgcggtgagt cgcagccagc cgcgcggtcc gctctgcaac tggccgaccg
    63001 cgctctcggc ttcatcgagc acgcgcgcga tgtgctggct gtgctcgaag tagacattgc
    63061 cggcctcggt gaggccgagc ttgcgggtgg tccggtgcat caactgcgtg ccgaggcgcg
    63121 cctcgaggtc ctggatcttg cggctgaccg tggtcttggg cagtcgcagc gcgcgcgccg
    63181 cgccgatgaa gcttccgtgc tcgaccacct tgacgaagac cagggtgtcg ttgagatcgc
    63241 gtgacatggc atccttcctg agttcgatta tcggcccacc gacgggacaa tattttccaa
    63301 aagtcggggc taatcaagcc cgttcgtcgc gcctatcctg tccgtgcaac gcaaccggaa
    63361 gtacggcaat ggatatggac attcaacggc aaacccacaa tcaagcactt gcaccgggcg
    63421 ctggggaccc gccggcgaac tatcccgccc atgggagtga aagtgccacc cgtgggacaa
    63481 tccggggggt gctagcggga cacctgccat ggaccggcgc tgccctggtc cttatcgcgc
    63541 tcgtcggcac gaccctcgtg gcattcgggt cgggtgcgaa ccacgatgcg ggcatcgggc
    63601 acatgcggcc gcccgacgtg agcgtcgcga ccgtgctcac gcagagcgtg cagccctggg
    63661 acgagttcac cggtcgcatc accgccgtcg agagcgtgga gctgcgcgcg cgcgtcagcg
    63721 gctacgtcga acgcgtcgcc tatgaagagg gccagttcgt ccgcaagggc gacctgctgt
    63781 tcctgatcga ccagcgcagc taccgcgcgg agtacgagcg ggcgagggcc gagctggcgc
    63841 gcgcgcgcag cgaggcagcg ctcgcgcgca gccaggatga gcgcacccgg gcgctcgtcg
    63901 cggcgcgcgc cgcatcgcag gaggaatccg acgagcgcag ggcggcggcc gaccagagtc
    63961 tcgcggcggt ccgggcggcc gaggcggcac tcgagcgtgc ccggctcgat ctcgagttca
    64021 ccgaggtgcg cgcgccgatc gacggccgcg cgggacgcgc gctgatcact gccggcaacc
    64081 ttgcgcaggc cgacagcacg ctgctgacga cggtggtgtc ggtcgatccg gtgtacgtgt
    64141 acttcgatgc cgacgagcag acctacctgc gctaccaggg actcgcgcgc agcggcgaac
    64201 gcgcggcctc cggcaatgcc gtgcgtatcg ggctggcgac cgagacgggc ttcccgcacg
    64261 ccggcaccgt cgatttcatc gacaaccagg tcgacgcggg caccggcacg atacgagtgc
    64321 gcgcactggt gccgaatccc ggtctgctgc tgacgcccgg gctcttcgcg cgcgtgcagt
    64381 tgcagggcgg cgaggagcgc gccgcgctgc tgatcgacga cagggcggtg ctgaccgacc
    64441 aggaccgcaa gtacgtgtac gtgctcgggc ccggcaacgc cgcggtgcgc aaggacgtca
    64501 cactcgggcg gacgtcgggt gacctgcggg tcgtcacggc gggtctcgag gccggcgaca
    64561 cgatcatcgt gaacggcgtg cagaaggtgt tcttcgaagg catgccggtg tcgccgcagc
    64621 tcgtcgcgat gggcgagtcg tcctcgggcg cggtgcgcgt cgccgcgggc tcgaactgag
    64681 ggcgcgcgat ggacttctcc aggttcttca tcgaccggcc gattttcgcg gccgtgctgt
    64741 cgatcgtgat cttcgctgcc gggctgatcg cactgccggg actgccggtg tccgagtatc
    64801 ccgacgtggt gccgccgacc gtgatcgtgc gcgccgtcta cccgggcgcg aacccgaagg
    64861 tcatcgccga gaccgtcgcg acgccgctcg aggaggcgat caacggcgtc gaggacatga
    64921 tgtacgtgaa atcggtcgcg ggcagcgacg gcgtgctgct cacgaccgtg accttccgtc
    64981 ccggcaccga cccggacgat gcgcaggtgg cggtgcagaa ccgcgtgagc caggcgcttg
    65041 cgcgcctgcc cgaggacgtg cgccgccagg gcgtcgtgac gcagaagcag tcgccgaccc
    65101 tcacgatggt ggtgcacctc acctcgcccg atcggaccta cgactctctc tacctgcgca
    65161 actacgcgac gctcaaggtg agggacgaac tcgcgcgcct gccgggcgtc ggcaatgcgc
    65221 agatgtgggg ttcgggcgac tatgcgatgc gcatctggct cgacccggac aaggttgccg
    65281 cgcgcggcct gaccgccgac gacgtggtgc gcgcggtgcg cgagcagaac gtgcaggtct
    65341 cggccggtca gctcggcgcg gagcccgccc cgaacggcag cgacttcctc gtgtcgatca
    65401 acgcgcaggg ccgcctgcag accgaggagg agttcggtcg catcgtcgtg aaaagcggcg
    65461 cggacggcga actcgtgcgt ctcgccgatg tcgcgcgcct cgagctcggc gccggcgact
    65521 acacgctgcg ctcgcagctc gacaatcagg atgccgtcgc gatcggcatt ttcgaggcgc
    65581 cgggtgccaa tgcgatcgcg gtatccgatg ccgtgcgcgc gcgcatggcg gaccttgcga
    65641 agcgcttccc gccgggcgtg gcctacagcg tcgtctacga ccccaccgtg ttcgtgcgcg
    65701 actcgatcaa ggcggtcatc gccacgctgc tcgaggcggt ggtgctggtc gtgctcgtcg
    65761 tgatcctctt cctgcagacc tggcgcgcct cgatcattcc gctgatcagt gtgccggtgt
    65821 cgatcgtggg cacgttcgcc gcgctgcacc tgctcggctt ttcggtgaat accctgacgc
    65881 tcttcggcct cgtgctcgcc atcggcatcg tcgtcgatga cgcgatcgtc gtggtcgaga
    65941 acgtcgagcg caacatcgcg gaaggcaaga ccccgctcgc cgcggcccac caggcgatgc
    66001 gcgaagtgtc gggcccgatc atcgcgatcg cgctcgtgct gtgcgcggta ttcgtgccga
    66061 tgtcgttcct ggaaggtgtc accggccagt tctatcgcca gttcgccgtc acgatcgcga
    66121 tctccaccgt gatctcggcg gtgaactcgc tgacgctctc gcccgcgctc gcggcgaagc
    66181 tgctgcgcgc gcacgatgcg ccgaaagatg cgcccacccg gctgatcgac cgcctgctcg
    66241 gcggcgtgtt ccggccgttc aaccgcttct tcgggacgag tgccgcccgc taccaggccg
    66301 cggtggcgca cagcctgcat cggcgcggcg cggtcttcgc ggtctacgcc gtgctgctcg
    66361 tcgccgcctg gggaatgctg caggcggtgc ccggcggctt catcccgacg caggacaagc
    66421 tgtacctgat gggtggcgtc aagctccccg aaggcgcctc gatcgagcgc accgacgccg
    66481 tcatccgccg catgagcgag atcgcgctcg ggaccgaggg ggtcgcgagc gcagtggcct
    66541 tcccgggact gaacgcgctg cagttcacca acacgcccaa caccggcgtc gtgttcttcg
    66601 gcctcgagcc cttcggtgaa cgcacgcgca gcgcggccga gatcaatgcc gagatcaacg
    66661 cgaagatcgc cggcatccag gaagcgttcg ctttctcgct gatgccgccg ccgatcctcg
    66721 gcatcggcgc gggctccggc tactcgctgt tcatccagga tcgcgccggg ctcggttacg
    66781 gcgcgctgca ggaggcggtg tcgcgattct ccggcgccgt ggcgcggacg cccggcatgg
    66841 gcttcccgat cagcacctac caggcgaacg tgccgcagct cgatgcgcag gtcgatcgcg
    66901 tgaaggcgaa agcgcagggc gtgccgctga ccgaactgtt cgacaccatg cagacctatc
    66961 tcggttcgac ctatgtcaac gacttcaacc gcttcgggcg tacctggcag gtgattgcgc
    67021 aggccgatgc gtcgttccgc gacgaaccgt ccgatatcgc caggctgcgc acgcgcaacc
    67081 tcgccggtga gatggtgccg ataggcagtg tcgtcacgct gggcgagacc tacggccccg
    67141 atcccgtgat gcgctacaac ggctacccgg ccgccgacct gctgggcgag gccgacccgc
    67201 gcctcatgtc ttcgaacgag gcgatggccg ccatcgcgca gatcgcgccg caagtgctcc
    67261 ccgagggcat gacgatggag tggaccgacc tcagctacca gcaggcgacg caaggcaacg
    67321 ctgcgctggt cgtgttcccg ctcgcggtgc tgctcgtatt cctggtgctg gcggcgctct
    67381 acgagagctg gacgctgccg ctcgccgtga tcctgatcgt gccgatgacg ttgctcgcgg
    67441 ccctcaccgg cgtgtggctc accggcggcg acaacaatgt cttcgtgcag gtgggcctcg
    67501 tcgtgctgat gggactcgcg tgcaagaacg cgatcctgat cgtcgagttc gcgcgcgagc
    67561 tggagctcgc gggccgcggc gtcgtcgagg cggcgctcga agcctgccgg ctgcggctgc
    67621 ggccgatcgt gatgacctcg atcgcattca tcgccggcac ggtgccgctc gtgctcgcgc
    67681 acggcgccgg cagcgaggtg cgcaccgtga cgggaatcac cgtattcgcg ggcatgctcg
    67741 gcgtaacgct gttcggcctc ttcctcacgc ccgtgttcta cgtggcgctg cgccggctcg
    67801 ctgcgccgcg actggagctg gctcatgcgt agacaccgat tgatgctggt gctgacgatg
    67861 gcgatggcgc tcaccggctg cgccgtggga ccagactacg tgcgcccgga tctgcccgtg
    67921 ccggggagtt ttaccgtcgc gccggctgcc gcaccggaca gtgcgggtga agtggtgctc
    67981 gcgcccgccg tggaccctga attctggcgc gggctcggcg atccgctgct ctcgcagctg
    68041 gtcgacgccg cgctcgagtc gaaccacgac ctgcgcatcg cactcgcgaa ctacgatcgc
    68101 gccaacgcgc tgctgcgcgg cgcgcgcttc gaccgttacc cgaccgtgac ggcgggcggc
    68161 tcggcagcca gcgagcgcgc gactgccgac gcggcgccgg gtgcctcgcg tgaggagcgg
    68221 gacctcgacc gctacggtgc cgacatccgc gccggctggg aactcgacct cttcgggcgg
    68281 gtgcgtcgct cggtcgaggc gcagcgggcc gaacgcgacg cggccgccag cgacctcgcc
    68341 gccctgcaga tcgtcatcgt gggcgaggtc gcgcgcagct acatggaact gcgcgggctg
    68401 caagagcggc tgcgcgtcgc ccgcgcgaat gcagtgaatc agcgcgacac actcgaactc
    68461 gtcgaggcac gcgtcggcgc gggtcgcgac accgacttcg accgctcacg cgcccgcgcg
    68521 cagctcgaaa cgactctcgc gcgcgtgccg gcgctcgaat cggcggtggc ggtcaccctg
    68581 caccggctcg ccgtgctcac cggccggcct cccgcggcgc tcaccgcgca gctcgcgagc
    68641 gccgcgccgc tgccggcact gcccgcgtcc gtcgatgcgg gcacgcccgg cgaactgctg
    68701 cgtcgccggc ccgacgtcat cgcggcggag gctcgcctgc acgccgcgac cgcgcgcgtc
    68761 ggcatcgcca cggccgacct gttcccgcgc ttcacgctcg ccggactgat cggcacgcag
    68821 gccatcgaca cggacgcgct cttcgagcgc gattccgagg tgcgattcgc cgcgctgggc
    68881 gtggactggt cgttcctcga tgtcgggcgc gtgcgcgcac gcatcgcggc ggccgacgcc
    68941 gacgccgcgg cggcactcgc gggctacgag caagccgtgc tgctcgcgct cgaagacacg
    69001 gagaacgcac tggtgcgtct cgccaaggcg cgcctcgagg accggcatct cgagagcgcc
    69061 gcgctcgaca gcgcgacggc ggcgcggctc gcgcggctgc gcttcgaggc gggcgcggtg
    69121 gacctgctcg atgtgctcga tgccgagcgc acgcaactgc tggcgcagga tgcgttcgcc
    69181 gacgcgcgca cgcgcagcgc caccgctgcg gtggggctct accgcgcgct cgccggcggc
    69241 tacggcagat agcccgggcc gcccggcggg tttgcccggc ccgcgcgggg ccctatagaa
    69301 tcaggttccc gcgcgggtgt agttcaatgg tagaactgca gcttcccaag ctgctaacgt
    69361 gggttcgatt cccatcaccc gctccatccc aactcgcccg ccgcgacgat gcgcgcgtgt
    69421 tctaggtccc cgtgctcagc cctcgcgcgg aagcaccgtg cgcacccggg cgatgaactg
    69481 gtggaactcg gtcatgaagc cgcgcaggaa tttctccgtg ccgctgtcgg tgacttcgaa
    69541 cttgtcgttg atcaggcccg gcgtgaaccg gatgtaggcc tccggcgcgt tcatctgcgg
    69601 tgaattgcag aagctcagta cgctgcgcag ctgctgctgc gccacggccg tgccgatcgc
    69661 gccggtggag gcgccgatga cgcccgaagg caggcgcgcg aaggagttgg tgccccacgg
    69721 ccggctcgcc cagtcgatgg cgttcttcag tccgccggga atggagcggt tgtattcggg
    69781 ggtcacgaac aggatggcgt ccgattcgct gatcgccttc ttgaagcggg tggccacttc
    69841 cgggaaatcc gcgtcgtagt cgtagctgta gagcggcagt tcattgaagg tgatctcgtg
    69901 gaagcgcagt tccttcggcg ccagcttgac gagcgcattg gcgagctggc ggttgatgga
    69961 gcccttggcg aggctgccga tcaggtatcc cacggtgtaa gtgctcatgg cggttcgagc
    70021 tccggtcggg tgggcaggtg tgtcagtcta gcccccgtca tgcattgcag tggtgattcg
    70081 ggtatgttcg cggctgctcc gccgccacac tcgcaggact cgtcatgacc accacgaagc
    70141 tgctgaacct gggcgccatc gtcgcgctgc ttgccgcctg cggcggatcg ggaaacgagg
    70201 ccgccgcgga tgccgtgccg gccgcgaccg gcaacgcgcc cgcagcaatg tccttcgatg
    70261 ggttcgttgc tacgccggtc gcccgcttcg acaagccctg ggcgatgact ttcctgcccg
    70321 acggccgcct gctcgtgacc gagatggcgg gtgtgctgaa gctctacgat gttgccggca
    70381 accgcaccgg catcgtgagc ggcgtgccgg ccgtgttcca caagaaccag ggcggcttct
    70441 ctgatgtggt gctgcatccg cagtactcca cgaaccaact cgtctacatc agttactcgg
    70501 aagccggtga cggcggcgcc ggcgccgccg tggcgcgtgc gcggctggtg ctcgatgccg
    70561 cgggcggcgg tgccctcgaa ggtctcgagg tgatctggcg ccaggtgccg aagaagagcg
    70621 gtgccgggca cttcggccag cgcatcgcct tcgaccgaga cggcatgctg tggataagct
    70681 cgagcgagcg ccaggagttc gacccggcgc aggacctcga caccaatctc ggcaaaatcg
    70741 tgcgtctcaa cgacgacggc agcgtgcccg acggcaatcc gttcgcggat cgcggcggcg
    70801 tgagcgccca gatctggacg ctgggccatc gcaatatcct cgggctcgcc ttcgatgcgc
    70861 agggacagct gtgggcccac gagatggggc cgaagggcgg cgacgaattg aatcgcatcg
    70921 agcgcggcgc caattacgga tatccgatcg tctcgaacgg cgatcattac gacggcaggg
    70981 tgatccccga ccacgacacg cgcccggaat tccgcgcgcc ggtggtcagc tggactccgg
    71041 tgatcgcgcc ggcgggcttc gtgttctacg acggtgacct gttccccgaa tggaagggca
    71101 gcgcgttcat cggcggcctg gtgtcgatgt cgctggtgcg cattgcgttc gacggcgaca
    71161 gcgcgcgcga ggctgagcgc ttcaacatgc agcgtcgcat ccgcgaagtg gagcaggccc
    71221 cggacggcgc gctctggctg ctggaagacg gcaaccgcaa cggccagggc tggctgctga
    71281 agctcacgcc gcgctgagcg tcacggccgc aacgggcggc cggccacata ggcccagccg
    71341 agctggaagc cgatgtcggg cgtgttgttg aagttctgca ggttctcggt cgccgcaaac
    71401 gtgaacagat gcgccccgcg ccggtggtgc acgccgaggc tgatctggaa cttggtcgcg
    71461 gtgagctcgt cgagatcggt gtcggcgcgc gtgtacacgc tcgggctggc gtagccctgg
    71521 atgatcgcat tggtgcgcgc ggagagcttc agctcgtagc cgagcacgag cgtggggatc
    71581 acctgggcat ccgaaggcat gaagcccccg gtgccggcgt aatacacgcc cgagaggttt
    71641 gcgtagaagg cctggcgctg ccagtagcgc tgcaacgtgg cctgcacgcc gaagtcgacg
    71701 cgtccgctcg agagcatttc gcgccggccg gccaccggaa tcttggcggc gccctcgagt
    71761 accagcttcc agttctccgg catcgcgatg ccggagtagc gcacgccgag cgtcggatcg
    71821 agcagtccgc cgctcgtcgg cgcttcgtac gccgcgtact gcgacgattt gaggtcgagc
    71881 aggatattga cgtcgttgcg gttggcggcg ggacgcccga agttgctgaa ggcgaacgcg
    71941 tcgtgaaacc gctcgatcat gccgtcgagg aagccgccct ggtaggtcgc accgctcagg
    72001 atcagataag cgctccaggc gtgtgaaagc tggtaataga gcgtcatgtc gaactgggcg
    72061 agttccagat cgatcaggaa gttctccccg ggcaggtcgc ggatcgcctg gaactcggca
    72121 gggccgagct ggcgccggcc cggcagcctg ccgaggtact tctcggtctc gcggctcagc
    72181 gcccaggtgt tctggtaggc gacttcggtc gcgacggccc aggcgcccgg ttcgccgaac
    72241 ccgcccagca ccgggcgcat gtcgagccgc aggaagccga acggggtcag gtcgcgcgcg
    72301 cgcagtgggc cgtagaagcc ggtgtcgtcg gccgccatgg cggtggtctg cgccagggct
    72361 gcgacgacgg tgagcgcaat ccatcgggtc atgtatcgct cgggtgaggg tgggagacca
    72421 tgcttggttg ccatgccgcc ccggtcaagt ggccagcgtg cgggtggcgt atccagcgag
    72481 gaaatcgacg aaggagcgca ccttggccga caggtgccgc cggctcgggt agacggccga
    72541 caccggcgag cgcgcgagcg cgtagtcggc gagtacgcgc acgagcgcgc cggtctgcag
    72601 ttccggggcc acgatgaaat ccggttcgat caccacgccg agccctgaag cggccaacgc
    72661 ggcgagcacg cggccgctgt tggcgcgatg ccgtgtcgcc acgcgcagct cgtggcgcgt
    72721 gccctgtgcc gattcgaagc gccacacgtt cggcagcggc atcagcgtgt aggtgaggat
    72781 ggcgtgcgcg ccgagctctt ccggcgtgcg tggcgtgccg tgccgctcga gataggcagg
    72841 cgaggcgcag cacagcggat aagtccagcc gatacggcgc gagacgagtc cctgatgcgc
    72901 gatcgcaccg atccggatcg cgagatcgaa gccttcctcg acgaggtcca cctgtcggtc
    72961 cgagaggtcg aggtcgaaca ccagttgcgg atgcagggcc gcgaattcgc tgatcgcggg
    73021 cgcgaggaag cgttcgccga aggtcactcc gcagctgagt cgcaagctgc cgctcggcag
    73081 taccgcgctc gcccgcaccg agctttcggc ttcctcgagg tcgagcagca acggcacgct
    73141 gcgctcgtag aacgattggc cggcttcggt gagcgacaga cgccgcgtcg tgcgattgat
    73201 cagccgtgca tcgagatgcg cttcgagttc ggccacgagc cgcgagacgg cggaggtcga
    73261 tgtgccgagc cgttcggccg cgcgcgcgaa gccgccgaac tcgacgactt tcgcgaaggc
    73321 ctcgatggcc ctgaatcgat ccatccccag attattgcag attcagcaac aatatttccc
    73381 aattgtgggt ctttatcccg cgatactgct ctaccactat ggcggcacgt cactattcaa
    73441 cgcgaggtac tcgtccatga agctttacta cacgcctggc gcctgctcgc aggcccccca
    73501 catcgccgcc agggaactcg acctgcccat cgataccgtg aaggtggatc tcgccaccca
    73561 caagctcgcc gacggcaccg atttctacac gatcaatccc aagggctaca taccgctgct
    73621 ggcactcgac gacggcacca cgatttcgga agccaacgtg atcctgcagt acttcgccga
    73681 tcagaagccc ggcacgctcg cgcccgtgtt cggtacgctc gagcgctgga aactgatgga
    73741 attgctgtcg ttcatcgcca gcgaagtgca caagggctac gccgtgctgt ggaatccgca
    73801 gacgcctgcc gaagtgcgca aagccgcgat cgacagactg ggcacgcgct tcgacctgct
    73861 ggcgaagact ctcgcgacac agccgtacct caccggcggc agtttcacga tcgcggacgc
    73921 ctacctgttc gtgctgctca actggacgaa gatccacaag atcgacctga cgccgtggcc
    73981 ggcgttgcag gcgttccagg cgcgggtggc tgcacggccc gccgtgcagg cgacgctcaa
    74041 ggccgagggc cttctgaagt aggcgatgac gacgcgactg ccgaccctgt ttctctcaca
    74101 tggctcgccg atgcatgccc tcgatgccgg cggggcaggt ccggcgtggg aggcgctcgc
    74161 cgcctccctg ccccgaccgc gcgccatcct gatcgcgtcg gcgcattggg agaccacgct
    74221 gccgatgctc acgggcggcg agcggctcga gacgctccac gacttcagcg gcttcccacc
    74281 cgaactctat cgcgtgcgtt acgacgtgcc gggcgcgccg gagctggcgg cgcgcgccgg
    74341 gcagctgctg aaggacgccg gctacacgcc ggcgatcaac gcctgccgtg gcatcgacca
    74401 cggggcgtgg gtgccgctga agtggatgta cccggcgcgt gatgttcccg tggtgcagct
    74461 ctcggtgcag actgcgctcg gcgccgcgca tcacctgggg ctgggcgagg cgctcgcccc
    74521 gctgcgcgac gagggcgtac tcgtgatcgg ctcgggacac gtgacgcaca atctgcgcga
    74581 ctgggcggcg catcgcggtg atcggcagcc cgccgactat gtcgtgcagt tcccggcgtg
    74641 gctcaatgag cggctgctgc gccgcgaacg cgacgaactc cttgcctggc aggaacgcgc
    74701 gccgtcggcg gcgcgggcgc atccgaccga cgagcatttc ctgccgatct tcattgcgct
    74761 cggcgccgcg ggcgccgacg ccgcgccgac gcgcttttac accgcgatcg acgccggcgt
    74821 gctgtcgatg gacgcctacc gcttcgactg aggcgtcagc gctgcaggcc gccggtcagc
    74881 acgatgcgca tcgcggcttc caccgaaagg tcggtgggct cgacctggct gcgcggcagg
    74941 tacaccgtgt agccgccgat ctggtaactc atcggcaggt agaccgcgac gagatcgccg
    75001 tcggtgtcga tccccggcaa cgcggcatgc tcctgcgtca cgaagcccat cacgcggccc
    75061 ggaccgaact tcaccatcac cacgcgttcg agctcgctct tgcgcccgct cgtgtcgacc
    75121 agcttggtga catcgcgcag cgcgccatac acggtcttta cgaccggcac gcgcatcacg
    75181 aggttctcga cgaccttcat gccgcggcga atcacatagg cgttgaccag cagcccgacg
    75241 atgagcagca cgatgaagcc ggccacgagg cccatgcccg ggatgtaatc ggcgggcgga
    75301 acgaaccagt ggatcggccc cgacagcagc ttctcggtcg tgatgccgag ccagtagatc
    75361 aggtagatgg tcagtccgat cggcaggatc gcgacgagac ctttgagcag gatgcgaccg
    75421 agctgactca atgggtggct cccggtaagg ggagcgtgat tctagtcgat cgccgccgcg
    75481 cagcgggcgg gcgatcgacc ccggggcggg ccctcagctt ccggtgacga ccttgccgtc
    75541 cttgaccggc aggcgcgaac cggggtcgtg atccatcttc accgtgtgct ccgcatcacc
    75601 caggcggtag cgtacgttga actcaccggt cggcacctgc ttcgcatcga acacggtctc
    75661 gcagcgggtc accgtgacct gctcggtgtt gccttcctgg atgcgctcct ggaccttgtt
    75721 gccggcatag ccgccggccg cggcacccgc gacggtcgcc gcgtcgcgtc ccgagccgct
    75781 gccgatctgg ctgccgagca cgccgccgac caccgcaccg acgaccgatc cggtcacctg
    75841 gtgcttgtcc ttgacaggct tctgctgggt cacgacttcg tcgcggcaga cctcgcgtgg
    75901 cgtacgttcg gtacgctgga tctcttccac ggagagaact tcggcgtagc ggtcgttcga
    75961 catcacgcgg taacccgcga gcgcggcaac gccggtgacc aggaccgcgc cggcgacggc
    76021 gccaatcatc gtttgtctgt tcatgcggcg aatcctagcc gggcgggccg gtgcggctgc
    76081 aggctcagat cacgtttgcg tgtgcgaatc gtcctacatg cgcgcaggag cgcgagccgc
    76141 ctcgggtgcg gcgatgatgg cctccggaac gaggcgcatc cagatacgct cggtctgctg
    76201 tgccggggtg ccggccgcac gcagggtggc gagcgcagca tcgtcagtca tcgcgccgga
    76261 tgagcgcagg acggcgagcg cgccagtgag ccgcccgaac cagacgctgc gtccgagcgg
    76321 cgacatcgcg cgcgcatcag cgtccgcgcg ctgctcctcg agcgccgcga gcgcgcgttc
    76381 cgacaatccc cagcttgccg cgacggcgcg cgccgcgcgc gcggcatgcc ggtcgatcat
    76441 ggccgcgatc agcgccggcg gcggtgtcgc ctgcgtcggc cccgcgtagc ggtcgaaggc
    76501 catgcgatac accatgatgc cgccgagtcc catcagcagt gcgagcagct gtgcggcgaa
    76561 cggatcgctg ccgtcgacga tcgccgcgta tgactcggcg gcaacgcccg atctgaacgc
    76621 atggtcccag gtcgtgtcgg gaaagcgcgc gtgtccgccc tgtgcgcgaa acaccggttg
    76681 caggaccgcc gccgcgacca gtcggcggat gccttcggtg ccgagcacgg cgaccgcgcg
    76741 gtcgatgctc tcgatcggct tgcgcgacat gcggtagtac gggctgttcg cgagacgcag
    76801 caggtcggcg gccagtgccg ggtcgcgcgc gatgatgccg gcgagctcgc gccgcgaagt
    76861 ctcggtgtcg ttcacggcgc gcaccagctg tggcagcagc atcggccggc gcggcatgta
    76921 gcggtcctgc gccgcgcagg cctcgatccc ctcggcaaac cacgccgccg cctcttcgtg
    76981 gccggcgggg acctgtggtt cgagctgcgg cgccgacagc gccagccggt gcagcgccac
    77041 ccaggcgtcg ccgggggcag gggcgcccgg cgcggtcgcc acgcgtgccg ccgcgggcgg
    77101 cgccgaactg gcaggcgcgg cgggcagggt ccgcgcggcg cgcgagcgtc gccagagcgc
    77161 tgccgcggca atcaatgccg cggcagcgag cagtgcggcg acgatcagcg gcacgtagcg
    77221 ggacaagcgg attctaggcg ggcttggcga tgccgatgaa cgccgccgcg agcagcccga
    77281 cccagatcgc gagtacatag ggccggcagg cgcgcagtcc ttccgacatg ttgcgatcgc
    77341 tctcctcggt cgcaccgctc gtgagcagct gcttgtaccc ctcgacgaac cgcggcagct
    77401 tcagccggat catgaggccg cagaagatca ggaacgcgaa gatgagcagc ttcgcggcga
    77461 gccacggata gggacggatg cgcccgtcgc cgtatgcata gccgaccgac gcgatcagcg
    77521 ccacgatcat gaagcagcgg aagtagaagt cgaactgcgt gagccgcttg ccgagcgcct
    77581 ggccctggta gtggtgcacg gcccagacga gcagcaccca cagcggaccg agcagcagga
    77641 tcagcggcag ttgtcccggc aggtgcggca cgtcgatgta ccaggcgagc agcccgccga
    77701 ccgacagcat gagtgcgagg cagtagcgcg gcagcatgtc gagttcggcg aagatcttga
    77761 atgcggtgag gcgcgcgtcc cgcgacagtt tcgggttgac gacgaagccg ctcgagtaga
    77821 acacgccggc gtcgccgccc agccagtaca cgaacagaag gatgtgtgcg aacttgacga
    77881 ccagataccc cagagactct tcgaacatgc cttgactcct gccgggccgc gactgccgcc
    77941 cagtgagccg cgatgttatc ccggcgcagg caaagtgcga actgcatcac atccgccggg
    78001 ggccaatggc gccgatcgat aattccgtca aacggcggtg cgtgccgatc ggacagaatc
    78061 ctcaaatgag cacgccgctg catgccgcca tccccggcga cctcgctccg ggactgttcc
    78121 gggacatggc cgaacagtgg ccgggcggcg tgattgtcgt gaatcgcgac ggcgtcattc
    78181 gctggttgaa cgcagcggcc cacgactggg cgccggcggg cgccggtgcg ctggtcggcg
    78241 cctgcatcga cagcatgcgc ctgccgtggc tgcaggatca ccacgacctg gcgatggtgc
    78301 tcggcggcgg ttcgcagcag ctcgcgccgc gcgcggtcat cgatcggcac ggtcgcgagc
    78361 gctactgcgg tttgcggctc atgccgctgc gtcgcggcca ggcgatatgt ggggcgatgc
    78421 tgttcgtcga agaaggtgtc gcggtggacg gctcgaccgt ggtgcaggcg ccgtcgattt
    78481 ccgataccgc ccgggccatt gccctgagcg catcgcaggt cggtgcctgg cactgcaacc
    78541 tgcagagcgg cgagggcgga gtcgacgccg cgtggtgcgc ggcgctccag ctcgacccgt
    78601 gcaccgggcc cgggcatctc gcacgctggg cgaagcatgt ccatccggac gacctcgccg
    78661 cgtatcggga gggctgggac gaattcgcac agggcgccag cgactccttc gaagcggagt
    78721 tccgcgcgct caccgacgac tcgcgctgga tgtggctgct gcagcgcggg caggtggtcg
    78781 agcacgacga ggacggccgg ccgctgcgcg ccgccggcat ctgcatcgag atcgacgcgc
    78841 gcaagcgcgc cgaggtcgcg ctgcaggaga aggagtcgcg tctcgccacc gcgttgtggg
    78901 gcgcgcgtgc cgccttctgg caatggaaca tcacgagcga cgtgtctgcg cgcagcgcca
    78961 tgtggtttgc gatgaccggc taccagcgcg aggaatggga acgcgagacg cgaccctggc
    79021 actcgcgcct gcatcccgac gatgccgagc gcgtcgagcg cacggtgcgc gaccatttcg
    79081 aggggcgcac ccagtcgctc gagatcgaat accggatccg cgtcgcctcc ggcgagtacc
    79141 gctgggtgca ggatcgcggt cgcgtcaccg aatgggactt ccagggcaac ccgaccattg
    79201 cgatcggcgt atcgctcgat atcgaatcgc agaagcaggc cgagctcgag ctgcgcgcca
    79261 gcgaggcgcg cctcgaaacc gcggtctggg gtgccaagat cggcctgtgg gagttcgact
    79321 tcgaaacgag tcatgcgcgc tggtacaacg actggtgcga ccaggtggac tttgatgcct
    79381 gcgagggcgc cgatcaccag tcgcgctggg aggcgaatat ccaccccgac gaccgcgccg
    79441 aggtggaccg cagcttcgac gaacacctgg ccgggcgcat cgggcactac gacgccgagt
    79501 accgcgtgcg cacccgaagt ggcgagtggc gctggatctt cgagcgcggg cgggtcacgg
    79561 cacgcgcgcc cgacggtgcg ccactgcgca tggtcggcgt gtgcatggac atcgaggagc
    79621 gcatggtcgc ggcgcgcgac gcgcgcgagg tgcgcgagcg cctcgagcgt gcgatcgacg
    79681 tggcgcgcat cggcatctgg agctgggatg tcgtcgacgg cgtgttccac tgcaacgagc
    79741 tttacgccga ctacgcctgc ggcggcaagg tcccgcgcga tgccgccgag cgccagcgcg
    79801 tgcgcgtggc gcacctgcat ccggatgacc tcggggagat ccgtgcactc gaggagtcgg
    79861 tgcacagcgg ccggcaggac gaattcgaat gccgccaccg ggtacgccac gcccagggcg
    79921 actggcgctg ggtactgcag cgcggacgcg tgaccgcgcg ttccgctgac ggcactccgg
    79981 cccacatggc cggcttcctg gtcgatgtga gcgacagtta cgaagaggtg atgacgcgcg
    80041 agaaggcggt cgcgaagctg cagaccctgg ccgagcacac gcccgattac ctgctgctgg
    80101 tcgatcgcga cctgcgcgtg cagatcgcca accgcggctg gggcgcgtac agcacggccg
    80161 acatcgtagg gcgcagcgtg ctcgatttcg tgccggaggc aaaccggcag gtgctcaccg
    80221 cgctgtatac gactgtgctt gccaccggca tctcggacga gcgcaccgtc gggcagccgc
    80281 gggccgatgg cagcggcatg cgactgttcg tgcatcgcgc ctcgccggtg cgggagaacg
    80341 gcgtgatcac cggtctcacg ctcgcgatga ccgacgccac cgcgcgcctg caggccgagg
    80401 agcgctcgcg cgcgaccgag tcggtgctga aaacgatcgc cgaggtgacc ccggactggc
    80461 tggtgatgct cgatgagaac cagcgctgca ctttcatcaa tcgcagcatc gctggtcacg
    80521 agccgggcga gatcatcggg cgcaggctcg atgaactccc ggtgagagtg ctgggcgacg
    80581 gcgtgcgcga gctgctccag ggcgtgctgc gcagtggccg ggcgcgcacc gaggagcatg
    80641 atttccacgc ccccgacggt acgtcgcggc gcttcgagct gcgggttcgc ccggtgcacg
    80701 ccgccgcggc cgggagcggc accggccgcg gcctcgtggt caccgccagt gacgtgacca
    80761 cacgcaagac nnnnnnnnnn gcgctcgagc gcgagatcct cgagatcgcc aatcgcgagc
    80821 agcagcgcat gggccgcgac ctggaagatg gcctgatcga ggatctcacc ggcatcgcgc
    80881 tgcagttgcg cgccgtggcc gggcagctgc gcaaggaagg ctcggaggcg cgtgccgacg
    80941 tggaggatgc catcgggcag gtcaactcgg cgatcgaggg cacgcgggtg ctcgcgagcg
    81001 gcctttcgcc ggtcggcgca gagcgcggcg gactcgtcgc cgccctcgaa gcacttgccg
    81061 cgcgcacgcg cgaccggcat ggtgtacaag tggatgtggt gacgcagttg gccaccccac
    81121 ttgtacttgt cgacggagcg ctcaatcatc tgtaccggat cgctcaggaa gcagtgatca
    81181 atgctgtacg gcacggacac cccaggcatg tgcggatcga gctcgcgctg gacggcggtc
    81241 gggtccggct gcgcgtcacc gacgacgggc gtgggctcgg acccttggcc gcgcagtccg
    81301 ccggccttgg acgccgggtc atgcgctacc gggcgcagat gctcgacggc gaactcaccg
    81361 tcaggccggg tgagtcgggc gtcgtggtgg agtgcatatg tccggtagcg aacaaccagg
    81421 gcgccgccgc gttctgatcg tcgacgacca tccgatcgtg cgccagggcc tggtccgcat
    81481 gatcgaagcc gctcccgacc tctccgtgtg cggcgaggtc gagaacgtgg tggatgccag
    81541 gcgagcaatc cgcgagcatc aacccgacgt cgtgattgtc gatctgaccc tggggcaggg
    81601 cgacggcctc gaactcgtgc gcgaagtgcg cgcccatcac ccgcgtctgc cgatgctcgt
    81661 gctcaccatg cacgaggagg ccatctacgc cgagcggatg ctcgccgctg gggcgcgcgg
    81721 ctacatcatg aaggaggcgg cttccgagca gctgctgacc gcactgcgcc gcgtcctctc
    81781 gggcagggtc tatctcagcg acgcgatggc gacccgcctg ggcgagcgcg gcgccatgcc
    81841 ggaaggcgag acggccgatg acccgctcgc gcggctcagc aatcgtgaac tgcaggtgct
    81901 gggcaagctc ggccggggga tgtcctcgcg cgagatcgcc agccaccttg ccgtatcggt
    81961 caagaccatc gagtcccacc gacagagcat caagcgcaag ctcaacctca cgtccaacgc
    82021 gcaactgctg cagtacgccc tgagcttcgt ggcctcgacc cgcgggggtg tctgagcccg
    82081 accccggggc cttaaaccct ggcggcccgg aacgcttcca acccaggcat aaggaggaac
    82141 ccttggcaga cggtgggact gcgggcgccc gcgccgtggc ggtggccgac atcgaggatg
    82201 cgctggtggc acgggcacgg cggggcgatg cgcgcgcgtt cgagcgcctg tatcgcgagc
    82261 acgccggccg ggtccacggc ctgtgcctgc gcatggcccg cgatccgcaa ctggccgagg
    82321 actacacgca ggagaccttc atccgcgcat ggcgtgcgct cgaccgcttc gaggcgcgca
    82381 gcaggatgtc gacctggctg caccgcatcg ccgtcaacgt gatcctcgag cgccgccgcc
    82441 ggcgcgacct gccgctggag ttcgtcgagg acgccgccga gtacgatcgt gacaacgaca
    82501 cgctcgatac ccccgtcgaa gaagcagagc tcgaggcggc gatcggctcg ctgccgccag
    82561 gggcgcgcga cgtgctgacg ctctgcggga tctacggcta cacgcatggc gaggccgccg
    82621 cgatgctcgg catcgcggaa gggacctgca aggcacagtt gcatcgcgcg cgcgccctgg
    82681 tgcgtgcgcg ggtggcgagt ggagaggcac gatgaacgag cacgaacgca aggtcgcttc
    82741 gccgggcgaa ctgccgcagg agatcccgcc ggcgcgcgac ctgtggcccg gcattgccgc
    82801 ggccatcggt gcggctgacg atgtgcgcgc cgtcacggcg ccgctcgagc cggcgcgtcg
    82861 acgcttcgcg gtgcacccgc tgctggggct ggcggcggcg atttcctgtg tggccgtcgg
    82921 catgtggctg ggtcgcgtct cggcgccgcc ggtcaccgtg gctgcggcac cgggcgagcc
    82981 cggcgccgca cctggcgggg ccgcccccgt gccggcggcg agcctggcgc tcgatgcgcg
    83041 cttcgatcgc gcgcgagagg aactgctgcg cgacgtggcc gagcgcctgg ccgccctgcc
    83101 gccggagagc cgtgcgaagg tcgaggcgag cctggcggac ctgcagcgcg cacgcacgga
    83161 catcgaggcg gcgctcggac gcgaccccgg caacgcgctg ctgcaggcga tgctggtgaa
    83221 cagttaccag gacgagatgc gggtactcgc ggcggtgcgc gatacctggg gctaccagga
    83281 ggatgtcgga gcatgagtat gcaattgatg aggagtgcgc tcggtgtcgc gctggccgcg
    83341 ctgctgctgg cgaccccggc gatggcgcgc gacgaccgga cggtcgacga gcgcatcgcg
    83401 gccgatgcgg acggcgaagt ggtggtcagc aatgtcagcg gctcggtgcg ggtcgagggc
    83461 tgggccggca aggaggtcga gatcaccggc tccctcggcg caggcgtcga gcgcctcgat
    83521 gtggtgcgcg acggcaagcg ggtcgtcgtc aaggtggtac tgccgaggac ttcgtcgcac
    83581 cgctcgtcgg cggatttgcg cgtgcgcgtg ccggtggcat cgcgcctcga ggtgacgacc
    83641 gtcagtgccg acagttcggt gcgcggcgtc accggtgcgt tgcgcctgtc gtcggtgagc
    83701 ggcgagatcg aggcccgcgg tgtcgcgagc gacagtgaga tcaagaccgt cagcggcgat
    83761 attcgcgtga cggcggcttc gacgccgatc aagctgcgcg cgaccagcgt cagcgggacg
    83821 atagtcgtcg acgacctgtc gggcgaagtc gagacattga cggtgagcgg ggagatgctg
    83881 ctcgacctcg atgcgctcac caacgtccgg gcacggtcca cctccgggga ctcgcgcatc
    83941 agcgggcagc tcgccaggga tgcccgggcc gagttcgaaa gcgtgagcgg tgacatgagc
    84001 ctcaagttcg gtgccgcggc cggttttgcc gtcgacgcgg agtcgttcag cggcgagctg
    84061 tcgaattgct tcggcgtgaa agccgaggcg gtgagcaagt acgcaccggg ggagcggctg
    84121 tcgctgacac gcggcgaggg cagcgcacag gtgcgcatca agacgatgag cggcagtgtg
    84181 cggatctgcg atcgctgacg gctagaatgg ctgtgtgcgc gtcgcgaaat gggttctgat
    84241 cggcatagcc gggctgctgg tggcggtggc gctcgccgtc ctcgtcatca cgcagttcgt
    84301 cgatccggcc cggttcaagg ggccgatcga gcggcaggtc gccgccgcca ccgggcgtga
    84361 tttcaagctc gcgggcgaca tcgaactttc cttcttcccc tggctcgcgc tgacgaccgg
    84421 cgccgcggag ttgccggcgc cggcgggatt cgccgacccg cgcttcctcg cctggcgcaa
    84481 tgcgcatgtc ggcgtgcgcc tgctgccgct gctgcgcggc gaactcgtgg tcgatcgcgt
    84541 acggctcgcc gggctcgacg cgcgactcgt caaggcggcc gacgggcgcg agaactgggc
    84601 atttggcgac gccaacggcg aggacagcgg tgacgccagt ggcgagggca cgctgcccga
    84661 catcgccggc atcgaactgc gcgaagcgcg cgtcacctat atcgacgagg cggccggcac
    84721 acagttcgaa ctggccgacg cgcgcatcga catcgaaccc atccgcagcg gtgtgccgat
    84781 gcgaatcgag gcggcggcga ccgcctcgat ggccgacctg cccgaacgca ttgccgtgga
    84841 actggaatcg cgcgtcacgc tgggcccgcc gatcgtgctc gccgacacga gcctgtccgg
    84901 tacgctgcgc gaaggtcgct tcggcgcggc aggcgtgccg tggcgcctcg ccgcgccgcg
    84961 cgtcagttac gacgccgcga gcggtgcgct cgctgcgccc gagtggcagc tgcgcttcaa
    85021 tgatgcgcag ctggacggcg agctcgctgc gcgactgggc gagccgctca ccgccgaagg
    85081 tcgggtcagc ctcgcaccgg tttcgctgcg cgagacgctc gccgccgccg gcgtggagct
    85141 gccgccgacg cgcgacaagg cggcctggga gcgcttcggg ttcgatgccg tattccgcgt
    85201 gtccggtccg gccttcaccg tatcgccact ggccattcgc ctcgatgaca cgagcctcgg
    85261 cggcacgatc gagcgggccg ccctggagga cagcctcatc gttttcgaac tgcgcgccga
    85321 tcgcatggac gtcgatcgct atctgaagcc cgcaggcacg ccgagcgaac cgttcgtctt
    85381 tccggccgag gcgctcaagg cgctgcgtgc ccggggcacg ctcgcaatcg atgaagccgc
    85441 gctcgagggc gtgcggctgc gcggtgtggt attcgaggcc gacacgccat gacggggatc
    85501 gccgacgcgc tcctgccctg gtatcgcgtc catggccgcc acgaccttcc atggcagcgc
    85561 gagcgctcgc cctatctcgt gtgggtctcg gaaatcatgc tgcagcagac gcaggtggcc
    85621 accgtgatcc cgtacttcga gcgtttcgtg gggcgcttcc ccgacgtcac gacactcgcc
    85681 gatgcgccac tcgatgaggt gctgcacctg tggtccggac tcggctacta cgcgcgggcg
    85741 cggaacctgc atcgcgccgc ccggcgcgtg cgcgacgagt tcgcgggacg ctttccggga
    85801 acctttgcgg agatcgagag cctgcccggc gtcggccgct ccacggccgg cgcgatcctg
    85861 gcgctctcgc gcggcgagcg cctaccgatc ctcgacggca atgccaagcg cgtgctcgcg
    85921 cgccgcttcg gcgtcaccgg ctcgcccggt gagcgcgccg tcgagcagcg cctgtgggcg
    85981 ctcgccgagg agaacacgcc gcacacggag gtcgatgcgt acacgcaggc gatcatggat
    86041 ctcggtgcca cggtgtgcgt gcgccggcgc ccgctctgtc cgatctgtcc gctgcgcgag
    86101 aactgcgtcg cgtaccgcag caatcgccag cacgtgattc ctgcgccccg cgtccgtgca
    86161 cgcagcgcgc ggcgcgtacg cgaagtcgtg atgctcgtcg cgcagcgcga ggacggcgcc
    86221 gtgctgctcg agcgtcgtcc ggccgagggc atctggggcg gcctgtggtg cctgccggag
    86281 ttcgcgagcg agacggcggc gcggggctac gcggcgtccc agctcgaggg cgccgagacc
    86341 gacgcacggg cgctcgcgcc ggtgccgcat gtgttcacgc acttcgatct cgtgatcacg
    86401 ccgctgctcg cgcgctgccg cgacggcggc cgagtcgcgg acgaggatcg cacgctctgg
    86461 tatgacatcc gctcgccggc gcgggtagga ttgccggccc cgatacagct gatcctcgcg
    86521 agcctgacat gagccgaacc gtgacctgcg tgatgttgaa aagggaggct ccgggcctcg
    86581 atcgccctcc ttacccgggt acgctcggcc agcggatctt cgagcaggtc tcgaaggaag
    86641 cgtgggcacg ctgggtcggc caccagacga tgctgatcaa cgaataccgc ctgacaccgg
    86701 tggatcccaa ggctcgcaaa ttcctcgagc aggaaatgga gaaattcttc ttcggcgaag
    86761 ggtccgtggt gcccgaggat tatcgtcctc cgcgttgaga tccatgtcgc cgtcactgcc
    86821 ggcggggccc ggcgcggttc ctgcccgacg atcgcgatcc gcatggcgcc tcgcgggcgc
    86881 gcgccggctc agactcccat cgggcgccgc ttgttgcgaa aggccctgat ccgcaggccc
    86941 gagatcacca gcacgacgcc aatcacgagc gcgaaggcgc caatgagcgt ggcaatggcc
    87001 agcgcgccgg cggccggctg cctcagcacg agcacgccga agatgacgct gaccgcgccc
    87061 gagagcccga gccaccactc gaagtcgatt tccttgcgga agcgtatcgc ggtgtagatg
    87121 tcgagcaggc catgcgcgat cgcccagacg gcgatcacga tcaggatcga gatcgcggcg
    87181 agcccgggcg caagcaccgc gacgccgccc gccgcaacgc tgatgacgcc gctccagaat
    87241 ggccagcccc agtttgtcgc cttgatggcg tagtagatcg aggcgatgcc atcgacgatc
    87301 cagtacgccg cgatcaacat gaggaaggcg agcaggctga tgccgggcca ggcgaacacg
    87361 agaatgccga agaggatggc gaccgtgccg cgcacgatcg gtacccacca gaaatcgagc
    87421 agggtcttct gcatgatcag tgctcccccc cggggcgcgg cggccattgt gcccgctagg
    87481 atggaccagc ccgtcggtcc cgggcaatgg cgtgtgggga atcaacgact tagcccgggc
    87541 ctcgcttgac gccccgccgc gccgcggctc taatacgcgg cccttcctcc aacgttggcc
    87601 aggtagctca gttggtagag cagcggattg aaaatccgcg tgtcgttggt tcgattccga
    87661 ccctggccac cactttccca attgtcctcg agttcgagtg cctgcccggg gtactcaggc
    87721 cgatgacggc gatcgcgccg cttccgtttg gctgaactcg catacgtagt taccgcaatg
    87781 ccagtgactt cacgacggtc gcaccgcaag atcgcgcggc cgatttctcc gccttgccgt
    87841 ttcgctgcgg tgctgacctg ggtcaaggtc aattaccatg actgcggcta gagtgtttac
    87901 acaggttcga tgatcggctt gcgcacgaag cagccggagg ctttcgctgc acatcggcgc
    87961 agaccgcacc tctcaaagcg atcactattt cggagaacca ccttgaagcc aacctacctc
    88021 aggcgtgcgg tcgcgaccgc ccccgtgatt cgtgctgccc tccgccaggc cctcggcgta
    88081 tcggtagcgt tgttgctgct gtcacccgcg caggcccagg acgagccggc gtcccagaac
    88141 gcccaggcgc aggaggccaa cggccttgct gaggtcgtgg tcaccgcgcg caagcgcgac
    88201 gaggccctgc tcgatacgcc gctttcggtg caggcgttca cttcggagga catcgcgtcc
    88261 gccggtctgc gcaatctcga agacgtctcc gcgatgaccg ccggcctgaa cttccagaag
    88321 ctgggcaact cgcaggcggg ccgctacaac agcgccatcc gcttccgcgg cctcgagttg
    88381 ctggtcacga ctccgaccag ccagacgggc tcgctgttca tcgatggcgt gtacatcctc
    88441 ggcggcgctg cgtcgatgca gttcaccgac gtcgagcgca tcgaagtgat ccggggcccg
    88501 caggccgcct acttcggtcg cggcaccttc ggcggcgcga tcaactacgt gaccgccgat
    88561 ccggcgagca gcttcggcgg ccgcacgagc ttcgagtaca gcccgaactt cggctccacc
    88621 ggcatcaatg gcagcatcga gggtccgctt ggcgacacgc tgagcggacg actctcggtt
    88681 tcctccagca agcgcggcgc gatgttcacc gccaccgacg gcggcgcgct cggcgaggag
    88741 cgcaccgaca tgatcacggg caccttgctc tggcagccgt cggatgcgct caaggtcaag
    88801 gtccgcgcgc tgtacggcga ggacgacgac ggcccggcct ccagcacctt cgtgccgttc
    88861 aggctctacg gcaactgccc gatcggcacg ccgatcaccg tgaagacgac gaccggcgag
    88921 gtggccacga ccatgcgtcg cgacctgcac tgcggtagcg taccggtcgt gccggtgagc
    88981 aataacacca ccttctacac cgtcgatacg acgctgggcc cgcagaacac ctacgacatc
    89041 atggtgctca acaacggtct gccgcccacc gacacgccgg cgatggacca cttcggcctg
    89101 cgctcgatct tccacctcta cacgggctcg gtcgactacg acttcaacga cgcgctgacg
    89161 atgtcggtgc tgctcggtta caacaagcgc gatacgacgc agatccgcga cgatgacagc
    89221 tacgacacca tgggccgggt gaccaagggc ttcctgagcc tggagtcgac gtcggtcgag
    89281 gcgcgcctgt actacgacgg cggcaaccgc ctgcgcggca tgatcggcgt gaactacttc
    89341 gacgaggagt ccttcggcga catcgatggt ggcatcggtc ttgtgaccaa cctgctcggc
    89401 ccgccgtcga tcggttccgc gggcggctcc atcgatgccg gcaagacgga tacgagcgcc
    89461 gtgttcgcct cggtcgaata cgatctgctc gagaacctga ctttgagcct cgagggacgc
    89521 taccaggaca tcgatacgga gacgctgggc ggcgcctatc cgggcccgta caccaccacg
    89581 aagtccaagc acaacaacac gctgccgcgc ttcctggtca acttcaagcc cttcgagagc
    89641 acgacgctgt acgcgaacta ctctgaaggc gcgctgggcg gcagccggaa cacggcattc
    89701 gcgattcgca cgccggcgga aaaggaagtc atcctggcgc aggttccggg cgtcagtgag
    89761 cagctcgcgc agcagaagct cgagtcctac gagatcggct ggaagcagag tctcggctcg
    89821 tcgcgctcct ggttctcgct gtccgcctac cagatggatt ggacgaacat cccgggctcg
    89881 gcgcaggtga ccttcatcag ccccacgacc ggcaatgcgg tgtccatcgg cgtcacgctg
    89941 ccgggcgatg ccgagctcaa gggcatcgaa gccgagctgc gctggcagcc gctcgatcag
    90001 ctggcgctgc acgcgagtgg cggctacatc gacgcgaagt acacggactt catcagcgcg
    90061 ggcctgaact cctacttcgg cctgggcggc gccctgggca gctacaaggc ggacggcgcg
    90121 accctgccgc gctcgccgca ggagactttc gcgctgtcgg ccacctggac cgacacgctg
    90181 aactccgact gggactggta cgtgcgcggt gacgccacgt acgcgggcaa ggcctacacc
    90241 gatgagacca acctgtcgtg gatcggcggc tactcgctgc tgaacagccg gattggcttc
    90301 gcccgcggag atggcctgaa catcgagctg ttctgccgca actgcctcaa cgaggacggc
    90361 tggcgcacgg gccgccgcgc catcgactgg ttcgacacgg cgccgttgcc gaacttcctg
    90421 acgcgcgtcg gctcgctgtc gcagccgatg gagaagcgcg agatcggcct gcgcgtgcag
    90481 ttcgagttct gatccgaggc gagcccctcg tgccgctggg cacgaggggt tgcatcgagg
    90541 agcgcgcctg gagcgcgcgc aaaaagcaag gccccgtttc cggggccttg ctgcgtctgg
    90601 acttcgtacg cgcccggcat cgcccgggcg cgtacgacca gcgtcagggc ttcttaggcg
    90661 gtttgggctt ctgggtcttc gcgtacacct cgaggctcga gaggttgggc ttgtcccaat
    90721 actcgcgcgg cggcgcctcg agcatatgcg ggtagtgctt ctcgcagtgc gcccggacct
    90781 tgggcttgaa gccgtcgagc gagtcgaacg actgcacggt cgagtggtag aggatgtggc
    90841 caggagcctg gcccatcagc atccacggca gccacggtgt gacgcgcgcc caggagccgg
    90901 tgtgcagcac cgaggtcttg ttgcggtcct gcaggtcggc gagtttgacc gtggtcgtga
    90961 agaactcgct gacgcggttc atcggaccgg cgctctctcg cggccacttg tccggctgca
    91021 gcgcggcctt gtagtagagg ttgatggact ggcgattctg caccatgcca ttgcccgcat
    91081 cgaagaagtt gttgctgaac ggcaccgact tccgctcggc ctggggattc aggccgccgt
    91141 aatccggccc gccaaccctg atgctttccg cgaagaagtg gttccagggg tcgttgatga
    91201 cgtccacgac ctcgacgacc tcgtcgatgt agggattttt ccagctgcgc aggatgtcgc
    91261 cggtggcgag gtcggaatag aagatcgcct cgcggtgcag cacacggtag ctgccgtccg
    91321 gctggcccca tgcgcgcgcc accgagacga cctcgaagcc gaacaggtcg cgcagtgccg
    91381 cgccatcgcg cactccgcag acgatgccgc tgacgctggc ccacttctgc ttgctctcgt
    91441 cgaggttggc atcgatgcgc acggcggcgt tcacgttgcc cttgggcgtc gtgaggtcga
    91501 tgtagggacc cttgaatgca gccggcacgg aggcgttcgc ggcgccaaag gcctggggca
    91561 cggcgctgag tccagccgcc agcgagccag tgagaattaa catgtcacga cgatccattt
    91621 gcacctcggg ctgattctgg ccgcaccatg cggttcggcg agagtgcgcc ggatcgggca
    91681 cccagtcaac gcgtgcaccg ggccggcgtg tcagatgact tcgaatcgtt gcagcagcac
    91741 gccgcactgg caaagcgaga cactacggat ttctacgaat gcatggggaa tcgtgcgtaa
    91801 tatagggtga tacgtttgat gtcgcggcgc agacggcatc cggtccggat tttcgaaagt
    91861 aacccctaca cgaaggtgca attgatcatg agtcgcccct ggacaggcat tgtccctgaa
    91921 gaggacatca ggcgttacga ggcagcgggt tttgggcggc cgacgggata cggtgcacgg
    91981 ccggcgctgc tgatcatcga cgtgcagtac cggaccgtcg gtacacggcc gatgccgttc
    92041 tgggatgcga tcgaggagtt tcgtaccagc tgcggcgaag tgggcaccaa tgcggttcgc
    92101 gagatcgcgc cactcctgaa gctcttccgg gacaatggtc atcccgtgct ctatccgcac
    92161 gtggctccca agctgagctt cgattccgga cgcttgggcg acaaagttcc cgcaatcatg
    92221 aacgtctcgg cgcaggggta tgagttcgtg ccgacgattg cgccgacgaa gggcgatatt
    92281 ctcgtgccga agaaacaccc gagcgccttc ttcggcacgc cgttggtcag ttatctgatc
    92341 aatctcgaaa ttgacacgct catcgtcacc ggctgctcta ccagcggctg cgttcgcgca
    92401 acggtagtcg atggctttgc atacaacttc aaggtcgtcg tgcctgaaga gtgtgtcttc
    92461 gaccgcagca ataccgttca tgcggtcagc ctgttcgaca tgtcttacaa gtatgcggat
    92521 gtacggcctg ttgccgacgt catcagcgaa gttgcaggac tcccggcccg caggccgcac
    92581 ccgggctgat tgcaccactc gacctgagtg gcccggaaca ggacgatggg tgctcgcttg
    92641 gagaaaatcc ccgtcacact gctgaccggc tttctgggta gcggaaagac gacgcttctg
    92701 aacggcatcc tgggtctgaa agactttgcg gatactgccg tcatcgtcaa tgaagtcggt
    92761 cagataggcc tggatcacgt gctggttcgg gcaggcgcgg acaacatcag gctgttggag
    92821 tccggctgcc tgtgctgcgc ggtcgagggt accttgaccg aaacgatgat ggatctctgg
    92881 agtcttcgtc atcgcggtga cataccggct ttcggccgcg tgatcatcga gaccagcggc
    92941 ctggccgagc ccgcgccgat cgcgcgctcg ctgatacagg acaggttcct ttcgtcgcat
    93001 ttcgtgcttg gccgcatact ggtcacgatc gatgccctcg atgggatccg gcagctatcg
    93061 agctttcatg aagctgtcga acagttggcg atcgccgacg ccgtaatcct caccaagaca
    93121 gatctggtgc acggggacgt ggctgagctg acagaagcca ttcggcgggt caattcgaca
    93181 gcgccgatca tccgggcgag tctgggtgcg atcgatccgt cggtgttgac cgggtcgtcc
    93241 agcttcaccc tcgaaacgcc cgcttcgtcg cagcatgttc actccgactt cgtatgccgt
    93301 gcagcttacg tcgacgaccc ggtgagctgg tctggtgtcg cggcattcgt cgatttgtgc
    93361 cggcgcactt atggtgccga actcttgcga tgcaaggggc tgctgcgcat agacggcgag
    93421 ggcggtgcgg ttctgcttca gggtgtgaat ggcgcattca gtaccacgac gctggagcgc
    93481 tggccggata gtgatccccg ctcgcgtctc gtgtgtattt tccgcggaga gccggaccgt
    93541 ttcgagagta tgctgacact gttgcacacc gaggccggga ctatgccgcc tgcctctctc
    93601 gacgaacttg aacaaggatc gatggcatgc gctttgatac gctgatttcc aacgggattc
    93661 tggtgtgccc ggatcgcggg ccgtttgctg cggatattgg cgtcaccggc gaacatatcg
    93721 ctgcggtgat gtctccgggg caaggtgcac aagccaggga aatcatcgat gcgtccgggc
    93781 agcatattct ccccggcttg atcgataccc atgtccattt cggtttcgcg gagcccgtga
    93841 ccgagtacac gagcgaaacg atctacgccg cgcaaggcgg attcaccaca gtgcttggtt
    93901 atttcctggg tagccggtcc taccgcgatg tgttcgccga ggaactgccc ctggccaccg
    93961 aacgcgcttt cgtcgatttc gggttccact tctgcgcggc gacggagaga cacctggaag
    94021 agctggcagc ttatgcgcac gacctgggcg taacctcttt caagtacttc atgaacttca
    94081 agggaagtga aggtctctat ctcggagttg aaggaacgga tgacggctat ttctacgacc
    94141 tgctcgcgaa agccgcgtca ctcggtgaag tcacggtcgt ggtgcatacg gagaatattg
    94201 agctggtgaa ccgtatgcgc cagaagttcc agtcaggtgg cagaaacacc ttgcgtgact
    94261 ggtctctggc caaaccgcca attaccgagg ctgaaccgac cgtgcgggcg atgatgcttg
    94321 ccgagcatct tggggcgagg atctacatcc ctcatctgag tacccgttcg gcgctgaacg
    94381 aggtccggcg ctggcgtgaa cgctatgcca acatctttgt cgagacctgc cctcactatc
    94441 tgacacacac gatggacgct gatatcggga gcatgggaaa ggccaacccg ccttttcgct
    94501 cggctgatga tgtcgcagcc atgtgggagg gcctggcaga cggttcgatc gacgtagtgg
    94561 catctgatca tgtgccccga aagcgttcga cgaaggacaa gccgctgtgg caggcgtctc
    94621 agggctttcc aggtacggcg acgatactcc cgatactatt ggatcagggc tatcaccggg
    94681 gacgcctgtc acttcagcga attgcccagt tgctgacctc gaggcctgcc gaaattttcg
    94741 gtatcgctga atcgaagggg cgcgtggcac cgggtctgca cgcggatctg accttggtca
    94801 atctggactt cgaacgtgtg gtgcgtgccg aggaactcgg ctcctactcg gactatagcc
    94861 tgtacgatgg ttggcgcttg aaagggtggc cgcgccgcac gattgtccgt ggtgcgactg
    94921 tcatgcagga cggcgtcatg acgggtgccg gcggccatgg ccggtatatt cggcgggccg
    94981 ctgccagctg atccgatctt ccgcttgaac ccggccggtc tcgggctttc gagacccgcg
    95041 cgtgcacgcg tacgccacct gctggcgtcg ggcacgcgcg gtcaccaggt gcgagacact
    95101 agcccttgtt gacccactgt cgtgcatgca ccgtggccgc ctggaaatcc cgacggccga
    95161 tgaacagagc aacaaacgca agtacaccaa atgcaaaata gacggtgctg aggctcattc
    95221 caacaccgcg ggtaccgggg aacaggttgt cggaaaggta gccgacgatg aaggagccgg
    95281 cggtaacgga caccagtcca gtcagcagcg cgtagatggc agtgaactga ccccgcagct
    95341 cattgggtgt gatctggctg attccggcca gcgccgccgc gacgcccatc gatcccgtaa
    95401 gggcagtcat ggcgtgaaag aacattgcga gctccccggt cggcatcatg aacttcagtg
    95461 tcccgaatat tgcccacaca ccaccctgca tggccgcgac aaggatcggc ccgtcggttc
    95521 ggccccgctt caggaaccag gacagaaacc acccggcact cagtgcgctt atgaccgcgc
    95581 acggcacgga gaccatagac agcaccgtcg caatctgtgt cggtgtccag tcatagagtc
    95641 gaatgaatgc tgtcggcacc cagccgagct gtgcatagac gatggcgatg ttcaggaccg
    95701 caccaacata cgttgcggcg taggctatcg ggtgccggcg atattgccgc aggatggcat
    95761 cgaagctgaa tttctgagtc gcggtggcgc gctcgcgccg gaccggctcg cgaaccgtga
    95821 acagcattag cagcgcgatg agcaatcctg gcaggccaac caggatgaac gtgatatgcc
    95881 aagtgctgaa gccgccgagc aacgtgggga accactcctg catcggtccc ccaatggcga
    95941 tggcgaagcc gccaagcagg tacgccattc ccgcgccgat cgcaccgccg accaggaata
    96001 ccccgtacgc ctgctggcgt ttctctggtg ggaaatagtc actgatcagc gaggaggagg
    96061 cgggcgtgac ggtggcttcg cccagtccta cgccgatccg ggagacaaat aacccgccga
    96121 acgaacgcgc aaagccagac acaatcgtca tgacgctcca cacgcctatg ccgatggtga
    96181 gcaacttcac tcgattccag cggtcgacga gcacgccgac cgggatagcg gccaacgtgt
    96241 acaggatcgc gaacgcaaat ccctgcagga tgccgatctg cgtgtctgtc acgcccaggt
    96301 cggtcttgat gtcgtccacc aggagcccga tgatgattcg gtccacgatc gcgaaggcat
    96361 aggcgacgag cagcaacacc gtcacatacc acgcgtaagc gggccgcggc caaggggacg
    96421 aagctgtcag tactgaagag cggacattgg cgctcgtcgt ttcatccggg gagtttatgg
    96481 cttgctccaa atatcaatga ccatggaaac cctgattcac tctcggcgtc caggttccga
    96541 acccatatcg ggtttcgaac ccatcgcagt cgacagtcgc agcaggctgt tcataatctc
    96601 atatgcgctc atctggcctg cggcggtcac cggcaacggc cgctggcggc tgggcgccac
    96661 gcggccgttg cttcgcttgc cgtacgctcc ttcggtcccg acggagcttt cctgattgaa
    96721 gatcagctgg tccggaaatc ggccagcagg ccacacccac gctgactgct ccactggcgc
    96781 gtcatttgga cgccttgccg atttccttat cgaggaacgc cttgacgtcg gcgaccgttt
    96841 cttccgggcg ttcgatgtgc agcaggtgac caccgcccgg gtagatgatc ttcttgacgc
    96901 tcttggcatt cttcaaggcg tcgacgaatg cctgcgcggt ttccggcgac agcgcattgc
    96961 tgttcccacc ccatagcacc aacgctggcg cccgcacgtg accgaggacg cgctgcggat
    97021 tgccgcgctc gtacattcgg gtcgctatga atccttcgcg gtcgcggccg atcacattcc
    97081 agtagtccac cttgcgttgg accatcactg gcgtgatctt gctgtgatcg ttgatgatgc
    97141 tcttgagggt cgagctgatc atgtcggtcg tggtcacctc cggcgtgaag acccttgcag
    97201 ggctgcgcgc ccgctcggtc gtaccgcccc tgccgccgta ctcgatgccc gccgagttct
    97261 gcaggaccaa cgcggtcacg cgatcagtgc gagtccccgc atagcgaaac gccaccagcc
    97321 ctccgaagga ggtgcccgcg atggcgaagc gctcgatacc caagtggtcc atgagggcat
    97381 cgaccagcat gaggtagcgg tcgaccgagt agttgccgct cggcaccggc ccggtcaggc
    97441 cgaaccccgg caggtccagt cgcaccactc gataactctt gctgagcacc ttcacccagc
    97501 cgtcccagtc cacgaggtct ccgactgaac tgtgcaccag gagtatggcc ggaccggtgc
    97561 cctggtcctt gtaatggacc ctgataccgt ccgagccttc gaggacgacg aaacgggaat
    97621 tctggtctgc gtacctcttt acgatctcgt catacgtgat cgtgccagcc gacttcggtt
    97681 cgcccggata cgcggtgtcc ggcgactgcg ccattccgat gaccggcgct gcaccaatca
    97741 agctggccag cagcaaggca aacatacgaa ctgaactcaa catgcgcgat tcctccgggg
    97801 gtcgcaatcg atcggggatc gcaatcgatg ctacgcggcg cctcaggcgc tgtcactggc
    97861 gtttctcggc tgcatcgacg agttccatgc agtgcacaag cgcctgcgca agatactcct
    97921 ccaccacctg cacgctgatc ccgcgacacc gcccgatcac cgcacgatca ccctgcgcaa
    97981 cgtttgccgc gcatgccctc cagacagcag cctcatcgaa tcctgaaccg gttaagggga
    98041 cattccgggt acccaaatcg cccatatcgc actctataga gtgttgggca tagacagact
    98101 ggagacgatg gcgggcaagt ctcgcgatta caggcagttc cggactcgag cgactgaacg
    98161 tcgggatcaa gcgttgcatc gatgtggtga gtatttaccc ttacgagacg cgctttcgtg
    98221 cgccccgtag gcgcgttgca gcccgggcag gtccaggtcg acgagtggca actgcagcat
    98281 cgccgcatgt cgcttgaacc gctgcgtttg ctgctccaca atgaaattct ctcgactgcg
    98341 ggccattgtc agccgagcga gagaccggtc acacgagatc tcacgactca ttcgcccatc
    98401 tgccggacgc gatcgagggc tggcgccggc aatcgtgcca atttttggag agtgacatga
    98461 agctttcggt catcctgtgg ggtatcccgc aagcgatgcg catatgcgca gctgtctatc
    98521 cgaagttcgc acagcgcctc aaagagcgtg actgcatcgc gcagttccag ttgaaggacc
    98581 agcccaaggg ccgctggata cagctcaagg gtggcaagat ttccagccgt gccggcatcc
    98641 atccgagccc cgatttcacg ctgttcttca agaacgagaa aatcgcctcg agtttcctga
    98701 ccccgccctt cgaccagctc gagcgcatcg atgcggccaa gaacttcaag atcaccatgc
    98761 agggcccgga tgccctggtg gactggttca tggcgctgat ctcgagcatg gagtcgtaca
    98821 cctggaccag cggcaccgcc atgggcaacg gtgtgacgcg ctacgccaac ggtaccaatg
    98881 gcgggccgat cttcgtctat gtgaaggacg gcaagatcat ccgcacgaca ccgatggacc
    98941 tcgacgacag cgacgcgggg tcgtacacga tcaaggcacg cggcaagacc ttcgtgccac
    99001 cgcgcaggac cacgctcgct gcacacggcc agtgccagaa gtcgatggtg tattcgaaga
    99061 accgcattct caagccgatg aagcgcgtgg atttcgatcc gcatggcgag cgcaacatcc
    99121 agaaccgcgg caagtcgaaa ttcgtcgaga tcagctggga cgaggcgctg gatatagttg
    99181 ccggtgagat caagcgctgc aaggcggttg gcccgggcgc catttgcgtc gccaacggat
    99241 cgcatcacca gtggggaaat ttcggccatt acctgagtgc ggccaatcgc ttctggaacc
    99301 tgatcggcag gaccaggctg atgcataacc cggacagctg ggaaggctgg ttctggggcg
    99361 cgatgcatca ttggggcaac agcatgcgcc tcggtgccgc ggagccttac ggcacggttg
    99421 aagactgcct gcagcaagcc gacatgatcg tgttctggtc cagcgacccc gacgcgacct
    99481 atggtttcga gggcacggag cgccgccgct gggccaaaga gctcgggatc aagatggtgc
    99541 acatcgatcc ctacatgaac cacacggccg cccacctcgg cggcaagtgg tttgcaccga
    99601 agcccggcac ggacgcggcc tttgcccagg cgctctgcca tgtctggatc accgaaggta
    99661 cctacgacaa ggagtttgtc gagaagcgca ccaccgggtt tgccgagtgg aaggcgcatg
    99721 tgctcggtga ggacgacgga atcccgaaga ccccggagtg gcaggaaggc gagaccggca
    99781 ttccagcccg cgaggtgcgc gcgctggccc gcgagtgggg tcgaaagaaa acctatctcg
    99841 gtgcgggtgg ctggggttgc ggcgtgggcg gtgcggggcg cgggccgacc ggcacgcagt
    99901 gggcgcggat gatgaccatc ctcggcgcca tgcagggcat gggccggccg ggttccaact
    99961 tcggcaacct gcagttcggt gcgccggtgg acatgaattt ctacttcccc ggctacgccg
   100021 agggcggcat gtcgggcgac ctgaccggca gcgccaacgc gaccaacaac taccagcgca
   100081 tgccgcacat actcacgatg agtacggtga agcaggcgat cccgaggatg tggttgcccg
   100141 aggcgatcac ccggggcaag gcgatgggtt acctgactgc ggtcgattcc ctgcagggtc
   100201 agttcatccc attcgggtat cccgcgcccg ggcatgtgcc ggtgcagatg ctcttcagat
   100261 acggcagcgc ctcgtttggg acgatggtcg acgggaaccg ctgggtcaac atgtaccagc
   100321 acgagagtct caagttcgtg gtgaaccagt ctgtctggtg ggaaggcgag acgtcgtact
   100381 gcgacattat cctgccggcc tgcacggtgt tcgagcgctg ggatatcggt gagtggtaca
   100441 acgtcggccc ggcctacgtg caccacatgt attccatgaa caaccaccgc gtaatctcga
   100501 tgcagcacaa gtgcatcgag ccgctcggtg agtcgaagtc ggactacgat atctttctgg
   100561 ccctcgccga gaggctcgat ctgggcgcgg tctactctga aggaggtaac tccgagtacg
   100621 agtggtgcaa gcgcgcattc gaatcatcgg atgtggcaaa gaacatgtca tggcgtcagt
   100681 tcctgaagaa gggctactac gtggtgcctc ccgaaccgga gggttcgcgg gcgccggtcg
   100741 ccatgcgctg gttctatgag ggccgcaaga aagatgtgcc ggagcccgct ccgctgccat
   100801 ccgactacct cggcaactat ggcgagggcc tccagactcc gtcgggcaag ttcgagttcg
   100861 tgccgactac gctgaaacgt ttcgaggatc cggaacgtcc gccgacgaac cggtacatgc
   100921 ggaccttcga gaatccgcag gacaaccccg agctcgcgaa gtatccgctg cggttgttgt
   100981 cgccgcattc gcgttatccc ttccacgtca tgggcgatga cgaaggctgt ttcctgcgcg
   101041 acatccgcga gcatcgcgtc aaggtgggcg agcactacta cctcgtggcg cggatcagcc
   101101 ccgaggacgc cagcgcacgc ggcatcaggg acgatgacct gatccggctg tggaatgatc
   101161 gcggctcggt cgtttgtgcc gcgcaggtca ccagtcggct gcggccgggc gtgatcagtg
   101221 cctcgacggc atcggccgaa taccggccgg tcggcgagcc gggcaagtca accgatctgg
   101281 gcggctgcgt caacatgctg aacccgagca aatccattac cccgaaaagc cacggtatcc
   101341 gcccgaacac gaccctgata caggtcgaaa aatggacggg tgtggatacc tggaagccgg
   101401 cggaggtggc gtgacatggc ggcaaagtgg aacctcattg tcgatgtcga gcgctgcaac
   101461 aactgtcgcg gctgttttct ggccgtcaag gatgagcaca ccggcaacga cttcccgggc
   101521 tatgcggcgg cacagccggc catgggcgcc aactggctga acatcgagcg caaggagcgc
   101581 ggcagctatc cgatcgtcga ggcgcacttc atgccggtga tgtgcaatca ctgtgacgat
   101641 gcaccgtgca tgaaggccgc taccaacggc gcagtcagca aacgtgagga tggcatcgtc
   101701 atcatcgatc cggaaaagag caaagggcag aagcagatcg tggatgcctg tccctacggc
   101761 gcgatctcct ggaatgagga gaagcagatt ccgcaggcct ggatcttcga tgcgcacctg
   101821 ctcgaccagg gctggagcaa gacccgcgcc gagcagtcct gccccaccga tgtgttcaag
   101881 tcggtgaagg tcgaagacgc gcagatgcag cggatcaaag agcaggaagg cctggaagtg
   101941 ctgcacccgg agttcggcac gaagccccgc gtgtactaca agaacctgca cctgatgacg
   102001 acgtgtctgg tcggcggatc ggtggcggcc gagatcaatg gtgtcgacga gtgcgccgca
   102061 gatgcggagg tcgtgctgaa aaaggatggt cgtgaaatcg cccggacgac taccgatact
   102121 ttcggtgagt ggaagatcga taagctcgct ccgaacagtg gcaggtacca gttggagatc
   102181 accggcgtat cgggccgctt ctcgatggaa ttcgatctcg gcgctgaaag cctctatctc
   102241 ggcgtgatga gactctagca ggctgttgaa gcatgccatg catcacgcag agcaccgcgg
   102301 gatgagacgc aggtggaacc gggcggagac tctgtccggg cgtatgcctc gcgacggccg
   102361 gccgtcaatc gcgccatcgt gcggaactct gtcgaacaag aagcagtaaa gcccaagcgt
   102421 aagccttcct acaatcgtaa ttgttcctga agtatggagt cggagatgcc caaattcctg
   102481 atttcgagaa gagagagcct cggcctcgcc gcatccgtgg cgggtgcggc agccgcatcg
   102541 gcactgggtg cggcgagcgc agcagccgcg ccggcagcgg cgaagaagcc caacgttgat
   102601 ttcgtcacca aggtcgattt caacagcccc gaatggaatc gcgatatccg cgcgcggatg
   102661 acttccgacg tggaccccac caaggaaaag ctcggttggc tcaagggcaa ggtatatggc
   102721 gtgcgcccca atgaggctgt gaggaccctg ttcgcgttcg aaggcttcag cctcgtgcgc
   102781 accaagcgcc ttccggacgg cagctggcgc cgcctgcttc gtgaaacggt attctaccgc
   102841 gacatcaaga ccggcaagat cctcaagact tggcacaacc cgtacacgaa tgagactgtg
   102901 cgtgtcgttc cgatcgcgaa caatccatac aacagcacgg ttactgccta ttcgcgcagc
   102961 ggcaagccgt atctcggcga gtggatcgaa gggccgaaca acaccctcac actgtactcg
   103021 ggcgtcaacc tcttctaccc gaacctgctt cagccgtcga agtggccgcg tgaatcggct
   103081 ggcgagttca atcaggttac cgagaatttc tactacacgg tcaatcgcgc ggacgcggag
   103141 aatccgaacc tgaccaatgt gccggtgctg ggcgcgtggc agcgcatcac gccctggctc
   103201 ccctggatgc tcatgggcgc ggcgccgggc cacatcgatt atttcagcat gttcgcgacg
   103261 atccctggcg gtcgcgatgg cctcaagaat ctgccgcagg atctggtcga ggctgctcgc
   103321 gagaatgatc cgctgatgct ttcggcgccc accgaagaca acctgccgaa cgaatccagc
   103381 ctcgaggcct atgccaagga gcagcagcct gcgccggtcc cgccaggctg gcagccgccg
   103441 cagcccccgc cgccgccgga gttcaccggc gatccggcga atctcggcag gcagggcgcc
   103501 gggccttcga actgacgctg tccttgcagc tccctcggaa gatcaggcgc gctggccggt
   103561 tctctagcgg accggttcag cgccgcctct ctaccgggcg ctccccaggc gcagggacgc
   103621 ccggtgccga caatgattgt cacggcaaag cgttcgtctg gcgggatagc gcagcttccc
   103681 gcgggcggcc cgctcgcccg tgagccgcgt ggcggcaatc acctcaccac attcgattcc
   103741 gctaccggtt ggcgaatggt gcgggccagc tcgtcatcgc gctcgccctt gaagaacgcg
   103801 ctcaggtcgg cggcgacccg ttgcgggtcc tcgacatagg ggtagtgccc gagttcgtcg
   103861 tacttcagga ccatcgaagg cgcgcccgag agccacagct cgaagacgtt ggcctcgagg
   103921 tgcgagaggg tgatgttctt cttgccccag atgatcatcg tgggtgcgcg cacctcggcg
   103981 agcgcggcct gcggatcgcc ggtgcggaac tgcttcagca tcacggcgct gtcttccttg
   104041 attccctcgc ggcggttcag gtcgtgcacg atcgtgacca gctccgcagg cgccggccgg
   104101 ccggtgccca tctgcgagtt catgctgtct tgacgggcct tgcgcgacga gtaatggctg
   104161 cgccaccagc gcgagagggc gccgccgcga ggacggttgg ggttggtcgc cgcggtgcgc
   104221 ggcaagccgg cgcagtttat gagtacgagg cgctcgacgc gctcgggcca gcgccccgca
   104281 tactggaacg cgacgatgcc gcccgaggag gtgccgacga ggctgaactt ctccacgccg
   104341 agtgtcgtgg tcaagccgtg caggaggtcg gcgaaacggt ccatggtgaa gtcctggctc
   104401 gggtcgggcc cggacaggcc gatcaccggg aagtcgatgc ggatcacgcg gaactcgcgc
   104461 gcaaggattt ccgcagtggc gtcccagacg cgcaggttca tgaacgaggg cggcagcagc
   104521 acgacgaccg ggccggcgcc ggcgtcggtg tagtggatgc gcgtgccgtc cacttccgtg
   104581 aacttcgagg tcggcaccgc atagcgggcc tcaagttccg cgcgcggcac ctcccggaag
   104641 ccatcgcaac ccacaaggcc cagccacacg caagcgagcg ccacgaggcc gcccgccttc
   104701 agagaaactc gcatgctcac gccctcgagg tagatacgta catagaccta gagcccggcg
   104761 ccgacgatcc gggtactctg attttgcctt caccgctcat tgatgtttcg atggaagggt
   104821 gacttgatgt cggcagcgca acatgtactg ctcgagcgcc tcttcgagga acgccggcat
   104881 tcgctggcag agttcttccg gcgccgtctg cgcgcgaccg ccgatgcggc ggaccttgca
   104941 caggaggtct atctgcgcat gctgcgcctg ccggcgacct cgcacatcga taacccggag
   105001 gcttacctgt tcaccgtcgc cgccaatctc gccagggagt acgcggtgct cgagcggcgc
   105061 cgcggcattc gcgttcggac ggaggactgc gagggtaccc ggcacatggt gcagcagccg
   105121 actttcgaaa acgatgtcga taccagcgca cgcgtggagc gcctgcagga agtattggca
   105181 cagctctcgc cgaaatgccg ggcagccgtg ctgctgcagt acaagcacgg cctgtcgtac
   105241 gacgaaattg ccacgcagct gggtgtctcg cgcaacatgg tcaagaagta cctctcgcag
   105301 ggcctggcgc attgccggcg ccggatgtcg cggctcgcct agatatcctt cagatatact
   105361 gcggcggaca tggatgcggc ggacatggaa atcaagggcc aggcatcggc agacatttcg
   105421 cagcaggctg ccgcctggtt cgtagccaac cagaatccgg atgaagctcg tgcacgcgcg
   105481 caggagttcc tcgcgtggct gcgtcgctcg ccggagcatg tgcaggagta tctggcggtt
   105541 gcgcagctcg cacgcgcgct cggtgactgc gccgaggcgc cgccgatcga gcagctggtg
   105601 cgcgaggcgg gaaccgcgac cgagaacgtc gtgccactcc acggctttcg gccgcccagt
   105661 gcagcgccgc agcgtggcca gtggtctcgc cggcgcgcat tgcccgcgct ggcggcctcg
   105721 ttcattgttg ccatcatcgg tctggctgtc tggttcagca gtgccatccc gcgaccgcag
   105781 gcatctgtcg cgcagtacat cacccggcat ggcgagcagc gttcgttgaa gctgcctgac
   105841 ggatcgatga tccacctcaa ttccgattcc gaggtcaagg tgggcttcga tacgacgcag
   105901 cggcgcgtgg aactgctcgc cggccaggcg ctgttcgacg tagagaagga tccggggcga
   105961 ccctttaaag tcattgccgg caggacggag gtcgtggctg tcggcaccca gttcgaagtc
   106021 tatcgccgcg gcgatgccac gcttgtgacg gtggtggagg gcgaagtcgc ggtcagcgcg
   106081 aatccgctgg ccggcggcgc gtccgaatcg cctggcaagg tggcgctcgt ggcgggccag
   106141 cgattgcgcg tgccgcaggt cgtcgacgca gcggcgagcg agagcatcga ggttccggag
   106201 atcgtgaacg tgcgcgagac catcgcctgg ccgcgcctac agatcgtagt cgactcgcgg
   106261 ccgctcggcg aagtcgtgca ggagttcaac cgccacgcta ctcccacaat cgtcattgac
   106321 gatcccggac tcgccgggcg ccggatcagt ggcgtgttcg acgtgcatga cggtgacaat
   106381 ttcctcgagt tcatacggcg catggaccac gtggtggtcg cgacgtatga agattccgtg
   106441 cggctcatac tcgacaacaa ttaaaaccgg tacccgttta ccggcatcgc acctcaccac
   106501 atagctagag ggcaggcgtc tgcatgctcc agactattgg gaggtcacga tacatgtccc
   106561 ggttttcacc gctcagaaca ttgctgattg ccgcgctgtt tgcggccggc gctcctggtc
   106621 tcgctctcgc gcaggccccc gctacgctgc gagccacgat tccggcgcag agcctggcgt
   106681 cggcgttgaa cgtccttgcg accgagaatg atttgcagct cgcctacgag tcggagatcg
   106741 tcgctggtcg tgatgccccg gctgtgccgg tgggcacgcc gctcgagcag gccctgcggt
   106801 ctctgctggg cgatagtggc ctcggttacg aattcatcgg cgagcgctcc atccgtatct
   106861 tcaaggctgc cggcgccacc aacaccacgg ccggctccac ccgtgtggcc gccgccgcag
   106921 gcgcggcccg cgacagcggc ctgctcgagg aaatcgtggt gagcgcgcgc aaacgcgacg
   106981 aggctctgct cgacacgccg ctttcggtgc aggcattcac cgcggatgac atcgagtccg
   107041 ccggtctgcg caatctcgag gacgtctccg cgatgaccgc cggcatgaac ttccagaagc
   107101 tgggtaattc gcagtccggc cgctacaaca gcgcggtccg cttccgcggc ctcgagctgc
   107161 tggtcacgac tcccaccagc cagacgggct cgctgttcat cgacggcgtc aacatcctcg
   107221 gtggcgctgc gtcgatgcag ttcaccgaca tcgagcgcat cgaagtgatc cgcggcccgc
   107281 aggccgccta cttcggccgc ggcaccttcg gcggcgcgat caactacgtg accgccgatc
   107341 cggcaagcag cttccggggg cgtgcaagcc tcgagtacag cccgaacttc ggctccagcg
   107401 gcatcagtgc cagcatcgag ggcccgctgg gcgacacact gagcgggcgc ctctccgttg
   107461 cgtccaacaa gcgcggcgcg atgttcaccg ccaccgacgg cggtgcgctc ggcgcggagc
   107521 gcaccgacat gatcacgggc acgctgctct ggcagccgtc ggatgcgctc agggtcaggg
   107581 tccgcgcgct gtacggcgag gacgacgacg gcccggcctc cagcacctat gtgccgttca
   107641 ggctccatgg caactgcccg atcggcacgc cgatcaccgt gaagacgacg gccggcgagg
   107701 tggatgcgac catgcgtcgc aacctgcact gcggcgaggt gccggtcatc ccggtgacca
   107761 acaacacgac gttctacacc gtcgatacgt cgcggggcac gttcaacacc cgcgacatcc
   107821 tggtggacaa ctacggcctg ccggccaccg atacgccgag gatggaccac ttcggcctgc
   107881 gctcgatctt ccacctctac acgggctcga tcgactacga gttcaacgac gcgctgacga
   107941 tgtcggtgct gctcggctac aacaagcgcg atacgaccca gatccgcgac gatgacaatt
   108001 acgacacctt cggccgggtg accaaaggct atatgagcct ggagtcgacg tcggtcgagg
   108061 cgcgcctgta ctacgacggc ggcaaccgcc tgcgcggcat gctcggcgtg aactacttcg
   108121 accaggacac cttcggcgac gtcgacggtg gcatcggcct cgtgaccaac ctgctcggcg
   108181 tgccgtcggt cgccgccggt ggcggctccg tcgaagcggg caagacggac acgagcgccg
   108241 tgttcgcctc ggtcgaatac gatctgctcg agaacctgac gctgagcctc gagggccgct
   108301 accaggacgt cagcacggat tcgctgcagg gtacctatcc gggtccgtac acggaatcga
   108361 agacctcgcg acacgacgct gtcccgcgtt tcctcgtgac ctacaagccc ttcgagaaca
   108421 cgacgctgta cgcgaactac ggcgaagcct cgctcggcgg cagccggaac gcgtcgtacg
   108481 cgatccgcac gccggcggaa caggaggtca tccgtgggct ggtgcccgga gtcagcgagc
   108541 agctcgccga gcagaaactc gaggcgtacg agatcggctg gaagcagagc ctcggctcat
   108601 cgcgctcctg gttcgcgctg tccgcctaca cgatggactg gatcaacatt ccgggctcgg
   108661 cccagttgac cttcatcagc cccacgaccg gcaatcccct gtccatgggt gtcaccctgc
   108721 cgggcaacgc ggagctcacc ggcttcgagg tggagctgcg ctggcagccg ctcgaccagc
   108781 tggtgctgaa tgcggctgtg ggcgtagtgg acgcggagta ctcgagcttc atcagcgcgg
   108841 gactgaactc ctacttcacc cttccgatgg gcgccaacta caagaccgat ggcgcgacgc
   108901 tgccgcgctc gccgcaggag actttcgcgc tgtcggccac ctggaccgac acgctgaact
   108961 ccgactggga
//
LOCUS       DAOQVY010000002        49017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_802_length_49017_cov_6.021792, whole genome shotgun sequence.
ACCESSION   DAOQVY010000002 DAOQVY010000000
VERSION     DAOQVY010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 49017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49017
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_802_length_49017_cov_6.021792"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1000
                     /gene="dmeF"
                     /locus_tag="PKN91_00520"
     CDS             38..1000
                     /gene="dmeF"
                     /locus_tag="PKN91_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011589743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HNS26579.1"
                     /translation="MHVHRTEHLGHDHVYLGDDHERNERRTWWVIGLTLVMMVGEVVA
                     GMLFGSMALLADGWHMATHAGALSITALAYAYARRHARNARFTFGTGKVGDLAGFASA
                     LILAVIALLIAVESLQRFLAPVPIAFTEATAVAALGLAVNLVSALLLRGGHSHAGNGR
                     RGHDHDHDHEHGHTASGADLNLRAAYLHVIADALTSVAAILALVLGRWLGWVWLDPVI
                     GVAGAVLIGRWSLGLMRATGEVLLDAADDTALAQAVREAIEAGDETVTDLHLWRVGPG
                     RHAAIVSLVAAQPLAPQEYKARLAHLPQVVHVSIEAHRCPEHPQ"
     gene            997..2652
                     /locus_tag="PKN91_00525"
     CDS             997..2652
                     /locus_tag="PKN91_00525"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNS26580.1"
                     /translation="MNLLALACLEAAVFFNANLHTGAEPVAGADAILVQDARISFIGP
                     TEEARRRAPEGAHFVDLAGATVVPGFTDAHAHLAGIGLREMAFDLDGTASLAELQARL
                     AARAASSPDDWITGRGWIESRWEPARFPTRAALDAVVADRPVLLERADGHAAVANSRA
                     LALAGIDASTPDPAGGRILRDAHGEPTGMLIDAATRLVERLVPPPSEADALRALELGA
                     ARSVRLGWTQLQDAGTDLAGITRLCRLYDEGRVKLRLYSAIGGPGSDADALLAGRIAR
                     HPCGERLTVRAIKLYMDGALGSRGAALLAPYSDARDSTGLLLNTETQLLPILEQALRS
                     GLQIETHAIGDRGNRMVLDLYERAFDAVPVAERSLAEPRWRIEHAQVLDRADLPRFSA
                     LGVIASMQPSHAISDLYFAPARLGAQRLAGAYAWQSLLASGAIVAAGSDAPVERGDPV
                     QEFYAAVVRRSPEGYAGEDWHREQRVTREQALRMLTLAPAQAAFEERERGTLEVGKLA
                     DFTVLSQDLLAVPDEAILATRVLMTVIGGEIAYRADLTAVTTE"
     gene            2737..3090
                     /locus_tag="PKN91_00530"
     CDS             2737..3090
                     /locus_tag="PKN91_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HNS26581.1"
                     /translation="MNKTERLTRRQLIGVTVTAAAGLSGAAAFAQAPAGAASFPKLDE
                     KEPTAVALKYVHDAKLVDPKKSPNYKPGQKCANCLQITGKDGDAWRPCNIFPKKLVAA
                     DGWCSVWVHKPGTAI"
     gene            3051..3971
                     /locus_tag="PKN91_00535"
     CDS             3051..3971
                     /locus_tag="PKN91_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNS26582.1"
                     /translation="MQRLGAQARHRHLSLRAPCVATLLCLIAAQVVAATAGAFSITDD
                     TGQPVALTRPAARIVALAPGATALLFAAGAGDRIVATIEYADEPDAARRLPRIGDVQA
                     IDLERLVALRPDVVVVTEGITSPLVIERVRGLHLSVYATRYSRLDDIAPSVARLGRLA
                     DTADVAEAAAARLAGTLAALRRQYAGRGPLEVLYQVWNRPIYSIGGPHIVTDALATCG
                     AHNAFADQRVAAPALGIEAVIARNPDVIVVSAPRAVASAWVEEWRKFPRLAATANDRL
                     YVFDDPRLDRMGPAAIEATANLCRILDAAR"
     gene            complement(3968..4450)
                     /locus_tag="PKN91_00540"
     CDS             complement(3968..4450)
                     /locus_tag="PKN91_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNS26583.1"
                     /translation="MPSFDIVSEIDLHELTNAVDQANRELAQRFDFRDTGAKYELEGH
                     TITLQAQVEFQLKQMLDILRIKLGKRGIDIVSLDIKDPETNLAAARQQVELRHGIDHE
                     TGRKITKLVKDSKLKVQCAIQGEKVRVTGKQRDDLQATMALLRKSPFDRPLQFENFRD
                     "
     gene            complement(4450..5694)
                     /locus_tag="PKN91_00545"
     CDS             complement(4450..5694)
                     /locus_tag="PKN91_00545"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNS26584.1"
                     /translation="MTRTTLFIAASVVLAACARPPADAPPVEDSVLAGQYRATLQLPG
                     GELPFGFELVRLGDRNLIYLQNGAERLAVEDVTLAGDTLEARMPGYENRISAKAEGAA
                     LRGELLLVKRGGANQVIPFIAERGVTHRFFATPAASAGSVSGRWAVTFAEADGKTSPG
                     VAEFRQEGAAVTGTILTPTGDHRYLAGEMRGNELYLSTFDGAHAYLYRAQLAADDTLA
                     GTFWSGLAWEQAFSARRDEAATLEGAPATAVRNAAERLEFTFPDLDGQPVSLSDPRFA
                     DKVVVITLAGSWCPNCHDEAMFLAPWYQRNRARGLEVISLMFEHFGDFEAAAAATRLF
                     RADLGIQYTTLIAGVSDRDDVAKKLPQLDAIYAFPTTLFLDRSGRVRHVHTGFSGPAT
                     GAHYDALTAQFDALLEQLLAEG"
     gene            complement(5691..8180)
                     /locus_tag="PKN91_00550"
     CDS             complement(5691..8180)
                     /locus_tag="PKN91_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26585.1"
                     /translation="MAGDYEKLGVFYLGRPYDPQAGAALPEPLLYDSRDLTTHAVCVG
                     MTGSGKTGLCLALLEEAGIDGVPAICIDPKGDLANLLLTFPQLAPADFEPWIDPAEAA
                     RKGQSVPDYAKVVANNWRKGLADWNQTPDRIAALRARVDLAIYTPGANTGLPLSVLRS
                     LDAPAEALRADVTSLRERVSGVVGGLLSLVGRDADPLQSREHILLSSIIEQAWRAGDG
                     LDLARLIAAVQKPPMDKVGALDLETFYPAKERTALAMALNNLLASPGFAAWLEGDPLD
                     AQRLLYTPEGKPRISIISIAHLSDPERMFVVTLVLNELIGWMRRQSGTSSLRALLYMD
                     EIFGYFPPTANPPSKPPMLTLLKQARAFGVGCVLATQNPVDLDYKGLGNTGTWFIGRL
                     QTDRDKQRVLDGLESALPGGAAGSRATLDRLMGSLTQRVFLMRNVHDDAPQLMQSRWA
                     LSYLRGPLTGPEISRLMADRKRDPSTAVAPATPAGASAAPAAMAATSSAAASGATASS
                     ARPAVPADVREYFAAALRGAGPIEYRPMILGTAKLHFVDARSKVDEWRTVTLLAPFSD
                     SGDEVEWPPAGEGAAPGVLADQPAADAQFATLPAAALRAQNYARWGKALAASLYESAR
                     AGIYSSKTLAMTSQPGESEGDFRARLAHAARERRDAAVEKIRDRYAAKRTSLADQVRR
                     AEERVGRERDQLSERRTQTAVSVGSAVLGALFGRKALSASTVGRAGTAARTASRVGRE
                     QADVARAEDNLAVLTQRLADLDAECEAALAVAATDFDASTLALEHKSIAPRKSDIAIG
                     EISLVWVPWRRGADGFPAPAWEGAPTAPLSV"
     gene            complement(8181..8522)
                     /locus_tag="PKN91_00555"
     CDS             complement(8181..8522)
                     /locus_tag="PKN91_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin assembly protein"
                     /protein_id="HNS26586.1"
                     /translation="MGFRELLDAWRTGGMSPRTVTEYAVRLPLDDAARLDALAELFPG
                     RTREQMITELLGLALDEMVSALPYERGSKVISTDDQGDPIYEDIGLTPRLIELARKHR
                     KRLQAGLPKAK"
     gene            8854..10239
                     /locus_tag="PKN91_00560"
     CDS             8854..10239
                     /locus_tag="PKN91_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HNS26587.1"
                     /translation="MRKTFDIAGDLDTPVSAFMKLGAFRPRFLLESVEGGERLGRYSF
                     IGFGDSLEYRLESDALYVDGVRTPAPADRDALLASLRAALARAARPQPEIAHVPLAGG
                     LVGYAAYDVVRYFERLPEHAQPVAGTPLMHYVAPRSMLVFDHLTRGIAVLHAGSAGER
                     ESLRREIVRALRGGLPNGQARGRHSEPVPSLTREAYIEGVRRAKEAITAGDVYQLVLS
                     ARFSGRHDLDPFQVYRALRLINPSPYLYYSRLGEHTVVGSSPEALVKLSAGVAELRPI
                     AGTRPRDDDPQRDAANEAALRADPKENAEHVMLVDLARNDLGRVASAGSVRVEPYRSI
                     ERYSHVMHMVSGVHGTLAPGRDAFDLFAAAFPAGTLVGAPKVRAMQLIDELEPVRRGL
                     YGGTIGYFGAQGDMDQAITIRTLVFNGDEYSFQAGAGIVADSDPDAEYDEVRAKGAAL
                     VRALALAEEGL"
     gene            10236..10817
                     /locus_tag="PKN91_00565"
     CDS             10236..10817
                     /locus_tag="PKN91_00565"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNS26588.1"
                     /translation="MSARLLLIDNYDSFTYNLVQAFMVLGAEVLVYRNDAIDVEAARR
                     LEPTHLCISPGPGTPRDAGVSIAMIRAFAGQIPVFGVCLGHQAIVEAFGGEIVRAGRL
                     MHGKTSTIEHDGRGLFAGLPQPCTVGRYHSLIAAPDRLPAELEVSARTAQGEIMAVRH
                     RKFDVEGVQFHPESVLTPDGPQLMANFLKTRHA"
     gene            10810..11820
                     /gene="trpD"
                     /locus_tag="PKN91_00570"
     CDS             10810..11820
                     /gene="trpD"
                     /locus_tag="PKN91_00570"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNS26589.1"
                     /translation="MRELLQRLIDREDLDEAAAGALLRGLADTATPPALAGAALAALQ
                     AKGVAASELRGLALAMRSLARRPEISPGAPTLDIVGTGGDRSGSVNLSTGSALLAAAC
                     GLRVVKHGNRSVSSRTGSADVLEALGLALPLDEQAAGSLLDQCGFTFLFAPYYHPAMK
                     AIAPVRAALGVRTAFNILGPLVNPAEPAFHLIGAYSAPVARLMADALAGMPIERAFVV
                     HGAEGWDEPTPVGEFMLIDVRPGSIVETIRDPREAGMARCAAAALVGEDASFNAAALQ
                     RTLQGEERGALRDALTLGAGLALELAGKARSLEEGIAAAAAAIDAGRAGGVLAALGAS
                     RR"
     gene            11817..12647
                     /locus_tag="PKN91_00575"
     CDS             11817..12647
                     /locus_tag="PKN91_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26590.1"
                     /translation="MSDFLATMAAASRARVHAALAACPLDAMAARARNSPPPPPLVLS
                     PGRFDIIAEMKLRSPAAGALRAASADDIAARVRDYARAGAAAVSVLTEPSRFDGALEH
                     LAQAAHALAPARVPAMRKDFLVDPYQVMEARVAGAGGVLVILRMLPRTDIEALLAAAA
                     SLELFALLEAFDEADIELAHELARHAGGRLLVGLNCRDLATLQVVPGRLEALAPRLPA
                     HLPRVAESGLDSAQDAARIAAAGYDLALVGSALMKRDAPGTLLADMLAAGRAARGSVP
                     "
     gene            12647..13240
                     /locus_tag="PKN91_00580"
     CDS             12647..13240
                     /locus_tag="PKN91_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNS26591.1"
                     /translation="MWVKICGMTTPAAVDAAVAAGADAIGFVFAPSVRRVAPAHAAML
                     AAPARGRARCVAVMQHPSQAEVTAMLAEFAPDVLQTDSEDFAALDLPASLERLPVLRA
                     GRELPTQLPRRVLYEGARSGAGEVADWTRAAQLCVRNVEVVLAGGLTPENVDSAVRAV
                     RPFGVDVSSGVESAPGVKSAARIAGFVAAARAAGREG"
     gene            13242..14507
                     /gene="trpB"
                     /locus_tag="PKN91_00585"
     CDS             13242..14507
                     /gene="trpB"
                     /locus_tag="PKN91_00585"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNS26592.1"
                     /translation="MTSLSTQEQAQLLADSIAGKFPDERGRFGPYGGRYVPETLIPAI
                     ERLEAGLRAHLHAPDFQHELADELRSWVGRPTALTHARRLGARWGAEVWLKREDLAHT
                     GAHKINNAIGQALLAKRLGAKRVVAETGAGQHGVASAAACARVGLPCTVYMGAVDMER
                     QAPNVGRMRLLGAQVVPVTEGDQTLRAAVDEALRDWVADPLETYYLLGSAIGPHPYPY
                     LVRELQTVIGREARAQILEQAGGLPDALIACVGGGSNAIGIFKPFIADASVAILGIEA
                     GGRGTALGENSATLALGRPGVLQGAYSVLLQDEHGQIQETHSVSAGLDYPGVGPEHAL
                     LQAAGRVSYESASDDEALAAVKECCEAEGILPAIESAHALAGARRWAAAHPGKRILIG
                     LSGRGDKDMPTLQRTLIAQLEAAAHGARS"
     gene            14504..15295
                     /gene="trpA"
                     /locus_tag="PKN91_00590"
     CDS             14504..15295
                     /gene="trpA"
                     /locus_tag="PKN91_00590"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNS26593.1"
                     /translation="MTAPQSLAPHERISAAIRAAAPHGPALVAFMTAGYPKRERFREQ
                     LAAVAGAADVVEIGVPFTDPMADGVTIQRSSREALAQGVTLEWILEELAAMPALRAPL
                     LLMSYLNPLLSFGFEKLAQAAERARVAGFIVPDLPFDEGGELAEALAARGIALVQMAT
                     PVSTPERLRRVCGASRGFVYAVTMTGTTGRSAAVPDDVLGYLQRVRAVSQVPVCAGFG
                     IRTREQVARLAGHVDGVVVGSALVEVLERGEDPGAWLRGLRQDGE"
     gene            15292..16494
                     /locus_tag="PKN91_00595"
     CDS             15292..16494
                     /locus_tag="PKN91_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HNS26594.1"
                     /translation="MTHQPDPRPTLGLAALLAFLSMVSPFALDTFFPSFPAIAQQFSL
                     SALQLQQTLTAYLLPYGLTMLLHGPLSDAYGRRRVILIGVSLFALASLACAFAPSFGW
                     LLAFRAVQGATAGAGMVVSRAIVRDLYSGHEAQRLMSLMTMLFGFAPAIAPVIGGWVH
                     VAFGWRAVFIFLVLLGATITFVAWWRLPETHPPERRFAFQFRSLITISWRIVSHREFL
                     LLALSTALHFVSVVAYIGAAPAIVLEQWGLTETQFAMLFVPLIGGFVAGAFTSGRMAG
                     RLSPHAQTRLGFLVSAIGGLLSTLALWLFSPVPIIVQQVLIFTAAYGVQIVSPIITLR
                     LLDLYPDTRGAVSSVQSFVSIMVAAFTMGVVVPALHGSLLLLALGSLVGALVGWSLWR
                     MADAAPAR"
     gene            16532..17233
                     /locus_tag="PKN91_00600"
     CDS             16532..17233
                     /locus_tag="PKN91_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26595.1"
                     /translation="MDTVDFITVPSRFRGPDHSANGGYFAGLVSARSASPVTVRLLSP
                     PPLDEPMLIQREGATLRVTHAETIVAEARAIDGGLPAAPEPPSYEEALEASRHYAGFH
                     THIYPNCFVCGMHRERGDGLRIFPGDARDGLVAAPWVPDASLAGDDGKVAPEFMSAAL
                     DCPGYFAVHLELPLLLGEFTVHIDRRVHIDEPCVVVGWRLGSSGRKHVVGTALYDEDG
                     QLCARARGLWIEPRV"
     gene            17247..17648
                     /locus_tag="PKN91_00605"
     CDS             17247..17648
                     /locus_tag="PKN91_00605"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNS26596.1"
                     /translation="MHPDVSKYNDAQDPADREICELLAREMDRNLPEAESRVWHGHPV
                     WFLDGNPIAGYSRQKPGIRLMFWSGADFDEPGLNVIGRKFKDASVFFNAASGIKKRDL
                     SRWLAKARTIQWDYKNVVKRKGRLERLKSPR"
     gene            18007..19641
                     /locus_tag="PKN91_00610"
     CDS             18007..19641
                     /locus_tag="PKN91_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HNS26597.1"
                     /translation="MVDFILDLVGYTPDQLLTQRRLLEPSFGNGDFLLPAIERLLRSW
                     AANDHLGDPLAALAPCIRGVELHKETFANTHAKVTAFLIDRGIQRSIAAKLTDTWLIH
                     GDYLLADLPAPFDVVVGNPPYVRQELIPDVLLHEYRRRYSTVYDRADLYIPFIERSLR
                     SLVDRGVLGFICADRWMKNRYGGPLRALVANDFRLKIYVDMTDTPAFHSEVIAYPAIT
                     VISREKSGPTRVAHRPRIAKDCLTTLSDKLTNEQIRPDCVEVRELAGITTDGEPWILE
                     SSDQMALVRRLERDFPTLEGAGCKVGIGVATGADKVYIGQFDELDVEPDRKLRLAMTR
                     DIVTGRVDWRGFGVINPFADQGGLVNLDNYPRLKRYLDTHKAEILGRHCAKKIPANWY
                     RTIDRITPSLACRPKLLIPDIKGEAQIVYEDGNLYPHHNLYFIISDEWDLRALQAVLL
                     SGIARIFVSMYSTKMRGGYLRFQAQYLRRIRVPNWKDVPVGVRQELKEAADARDVVAC
                     DRAVFKLYGLTHGESAALGGVVKPNESGPSGLRPQS"
     gene            19604..20344
                     /locus_tag="PKN91_00615"
     CDS             19604..20344
                     /locus_tag="PKN91_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017173568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaeR7I family type II restriction endonuclease"
                     /protein_id="HNS26598.1"
                     /translation="MSLDLQDYDRKASDAVRAFWANREKARQKQIQDGKIDQGERAGV
                     TAGKNMDGFVDLVSDIVKANGLAHADIHQHRPLLALPGYFRPTKLWDLLVIYKGHLIA
                     AIELKSQVGPSFGNNFNNRTEEAIGTAHDLWTAYREGAFGKQPRPFVGWMMLVEDAKA
                     SRVSIRDSSPHFPVFSEFQGASYIKRYDLLCQRLTQERLYSTAALMTTPRSAVNDGEF
                     GAESDMTSFKTFVTRLAGHIAAEGARLG"
     gene            20421..20912
                     /locus_tag="PKN91_00620"
     CDS             20421..20912
                     /locus_tag="PKN91_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26599.1"
                     /translation="MDLTTEQIIELWFHYEEVAMHFNELIIQYRLQLMGGVGAIGALS
                     AYLIGDKVEDRQRRYRLRLVASLGLFVLVLAAALLDLLYYNKLLLASVDVLIDFEKAH
                     PPLNMSTRIAEKIAGQGLPVICGAYGVVLGSLGLFTLWSWRQHSKCTERSLGDPTDTS
                     CVG"
     gene            complement(20958..22598)
                     /gene="ubiB"
                     /locus_tag="PKN91_00625"
     CDS             complement(20958..22598)
                     /gene="ubiB"
                     /locus_tag="PKN91_00625"
                     /inference="COORDINATES: protein motif:HMM:NF003404.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HNS26600.1"
                     /translation="MKLRVLARLIEIQRVLVRHGLDDFVRATHLYRPLRYLLLASPWT
                     WFARRHGGSRGERLRLALEELGPIFVKFGQAVSTRRDLLPQDIADELAKLQDRVPPFP
                     GAEARAAVERAYGQPIGEAFASFDETPLAAASIAQVHVAHLADGREVIVKVLRPGMHA
                     VIERDLEVLEALAELAQRYWSLAPRLRPREIVDEYRKTILDELDLMREAANAAQLKRN
                     FEGSSLLYVPEVHWDLCRVDVMVMERIHGIQISDMAQLRAKGTDLRKLAVNGVEIFFT
                     QVFRHNFFHADMHPGNIFVLADDPANPRYAAIDFGIVGTLDPRDQHYLAENFLAVFDR
                     NYRRVAELHVESGWVPRDTRIDEMESAVRTVCEPIFRKPLKEISFGTVLLRLFEISRR
                     FDMRIQPQLILLQKTLLNVEGLGRELYPDLDVFATASPVLREWMRERVSVRRIAGEIR
                     THLPDLIAMLRVLPPMVQGAVQRAQDGTLRLPVAADGLDELREELRGSQRRRDAMLIA
                     AAALLGGIVWLALERDPAWPGWLLTAAAVVGAFFARRG"
     gene            complement(22595..23209)
                     /locus_tag="PKN91_00630"
     CDS             complement(22595..23209)
                     /locus_tag="PKN91_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNS26601.1"
                     /translation="MITSAVQQLLNRNLPRSPQARALCEELAGRRAALTIEGTGLRLV
                     LASDGQRITLTHETRPEGEASPPADAEIGGTPLNLLALLGTDAEGVIRRGDVRIDGDA
                     QLAQRFRELAHLLRPDIEEELSQLVGDAAAHGLARAAAGVLDFGRQVAATGSRNVAEY
                     LAHEHADLVPRAEADAFFADVDRTREDVDRLAARIELLVRRDQP"
     gene            complement(23217..23972)
                     /locus_tag="PKN91_00635"
     CDS             complement(23217..23972)
                     /locus_tag="PKN91_00635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS26602.1"
                     /translation="MSQPSADPTIDFGYERVRREDKARRVRGVFDSVADNYDLMNDLM
                     SGGVHRIWKRFALSITGLRPGQYALDVAGGTGDIALGLARQVGPGGLVVLTDINAAML
                     AHGRDCLLDAGHLRGIECVQANAEQLPFADGSFHCVTIAFGLRNVTDKAAALASMRRT
                     LKPGGQLIVLEFSKPIDALQPLYDAYSFGLLPLLGRVVAKDEASYRYLAESIRMHPDQ
                     ETLLGMMRGAGLEGCRYHNLSGGIVAVHRGYRY"
     gene            complement(24161..24460)
                     /locus_tag="PKN91_00640"
     CDS             complement(24161..24460)
                     /locus_tag="PKN91_00640"
                     /inference="COORDINATES: protein motif:HMM:NF017922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNS26603.1"
                     /translation="MAARICTEIRLRQHSRLLKVSFDDGRSFELPFEYLRVNSPSAEV
                     QGHGPGQRVKVVGKEGVGIRAVQPVGQYAVRLVFDDGHDSGLYTWDYLYELGERL"
     gene            24579..26393
                     /locus_tag="PKN91_00645"
     CDS             24579..26393
                     /locus_tag="PKN91_00645"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS26604.1"
                     /translation="MPPTDTSRPDYFHRVVDCQWACPAHTDVPEYIRLIAQGRFTDAY
                     MVNRASNVFPGILGRVCDRPCEPACRRGRVEDKPVAICRLKRVAADHKDEFRDRLPQI
                     PEQGNGKRIACIGAGPVSLTVANDLLPLGYQVTIFEQFREPGGLMRTNIPSFRLPQTV
                     LDEEIGAIVDMGAELKLNHPIKSMRALLEGGGFDAVFVGSGAPKGKELKLPGREEGAA
                     NIHIGINWLESVAFGHLNAIGREVLIIGVGNTAMDCCRTSLRLGAAHVKVMARKPRQF
                     FKASAWELDDAEEENVEIIVNRSPKRFVIEQGRLTGMLFGVMEYDIDAGGRITAERVA
                     GEEFLPADDVILAIGQENAFPWIERDLGIEFDKWSVPKVDATTFQSTRPEVFFGGDAA
                     FGPKNIIWAVEHGHQAAISIHRHCQGVAVTERLAPGVNLQSRKMGMHEWSYKNDYNPV
                     ERRLMPHVSLKERFKKLNIEVELGFTAEQAAAEVQRCLNCDIQTVFEAKLCIECDACI
                     DICPTDCLTITTDGTEDDLRTRLRAPAANLEQALYVSAPLKQTGRVMVKDEDLCVHCG
                     LCAERCPTAAWDMQKSYVAWPHAKDQPEKCQPSLPKIA"
     gene            26363..28225
                     /locus_tag="PKN91_00650"
     CDS             26363..28225
                     /locus_tag="PKN91_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNS26605.1"
                     /translation="MPALTAQDRVNDFVIKIATVNGTGSASANALLMKAIFRSGVPVM
                     GKNYFPSNIQGLPTWYEIRVTRDAHTARSGRIDLMVAMNAETYGKDMREVDSGGYLIY
                     DSTWPRPALLSREDITVLGVPLARLCNERFKGVRTRILMKNICYAGVLAALLDIDLER
                     MRELLAETYAKKPQLADSNMEAVQLGYDYAKQHFNCPLPLRVAPLDRTAGHILIDGNT
                     ATGLGAMFAGATVGAWYPITPSTSVMDAYKAFCDKWRVDPETGRRNFAFIQAEDELAA
                     IGMVLGASWNGARAFTATSGPGISLMNEFLGLGYYAEIPAVVFDVQRVGPSTGMPTRT
                     QQCDLLSCAYASHGDTKHVCLYPASPEECFYFAIAAFDLAERLQTPVMVLSDLDIGMN
                     DWMCPEFKWDDSYRPDRGKVLPLEEIVKLEKFYRYLDKDDDGIPYRTLPGVHPKAAYF
                     TRGSGHTQYGAYTEDSAEYQIVVDRLLRKFGTAKTLVPKPVIEMADKRTDVAIVAAGS
                     SDGAVREALVVLAGMGIPVNYLRVRAFPFNDEVERFLAEHRLVFVVEQNRDAQLRSLL
                     TLETQVEKSRLRSLLHYSGLPISSSFIIEGVLTEVAPRPLRSVAPVHATGTPAE"
     gene            28230..29285
                     /locus_tag="PKN91_00655"
     CDS             28230..29285
                     /locus_tag="PKN91_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNS26606.1"
                     /translation="MTFIPKPKVRHPSLPRNDLGLTRRDYEGVLSTLCAGCGHDSITA
                     ALTEAIWGLSLEPQNIAKLSGIGCSSKTTAYFVGGAHGFNSVHGRMPAIAMGANAANR
                     KLSYIGVSGDGDTLSIGLGQFCHAIRRNLNMLYVIENNGVYGLTKGQFSASADIGTKA
                     KKGEKNLQPPIDPVQLAMSLGASFVARSFSGDKRQLVPLIQAGLRHRGFALIDVLSPC
                     VTFNDHEGSTKSYAYTREHYEPVVNADFVPLEREIVADYAEGESLPIVMHDGSRILLR
                     KFAQDYDPTDRVAASAFIQARQKQGEFLTGLLYIDESQPEFHEVNGTPDAPLNSLPFA
                     ALSPGAQGLAKILSRYR"
     gene            29343..29462
                     /locus_tag="PKN91_00660"
     CDS             29343..29462
                     /locus_tag="PKN91_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HNS26607.1"
                     /translation="MGKGDPRSRRGKIYQGSFGKTRPKKKAAKKKAAGKSTRK"
     gene            complement(29452..30549)
                     /locus_tag="PKN91_00665"
     CDS             complement(29452..30549)
                     /locus_tag="PKN91_00665"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNS26608.1"
                     /translation="MTTYAAITGWGKCLPPAVLSNQDLAGMLETSDEWITSRTGIRER
                     RISHVPMAELAHVAAARALACAGMAGREIELIVFGTCSFEDQVPNMASGLQQRIGAQG
                     CAAMDVNTACTSFLYGLSSATAMIRNGVVSNALVVGAEIISPYMDWSDRNVAVLFGDG
                     AAAVVLQATDSEEGLLAEKLGCYGDARETLRIHGMGGNYANQGRLYGVTSWQFEGQEI
                     FKRAVLGMSEASAAALGKRGLTPDDIQLAVPHQANLRIIEAVAKRAGIPMERVFTNVQ
                     RYGNMSSATVPVGLCEALESGRIEKGGWVLMPAFGGGLTFCAHLVRWGNRITPLDTTD
                     IELPPCDRTAVELIHEVAARKVTRAPDPATS"
     gene            30847..31038
                     /locus_tag="PKN91_00670"
     CDS             30847..31038
                     /locus_tag="PKN91_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HNS26609.1"
                     /translation="MKYQSTGDAWLAIAVIFLVIAIVAAVFGFGGIAAGAAGIARILF
                     FVFLIAFAVTLLVGLFRRA"
     gene            31098..32534
                     /locus_tag="PKN91_00675"
     CDS             31098..32534
                     /locus_tag="PKN91_00675"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HNS26610.1"
                     /translation="MTEGNGLPWRQYRSVHLDFWDGLRLLPGSIWLPIVAISLLALGV
                     FLMNEWSMGRIRIAAEGVNRTLGMQNEVIALRSQLVDLESAQRGYLLTTNRRYLAPYE
                     AALPELHVISSKLRTLAAADAEVLQHVTRLEELRGIKIAEVTASISLTQRGDRASAMA
                     ILRTGEGRKVMDEFRAAAHELLQLLDTRLEALRAEQARSLVLSRYAFAALALLALALI
                     LLVVRLFVANSLRREEQRHEEEHERRRLEVVIEDRTRELSRLTTHLQSASEQEKADLA
                     RDLHDELGGLLTAAKMDLAWLQGRSITMDPEVREKLDELVRTVTEAMHLKRRVVENLR
                     PALLDHFGLPTALEAYFDETCKRAGLSCRTSIPEDTAPIPQQLAIALFRVGQESLTNI
                     IRHARARNVEMQFEVTESGYHLTVSDDGVGIDPERARSSHGLSGMRHRIVSLNGSFDI
                     QSVPGEGTRLDILVPFAEAGVQGYQLSA"
     gene            complement(32519..32998)
                     /locus_tag="PKN91_00680"
     CDS             complement(32519..32998)
                     /locus_tag="PKN91_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS26611.1"
                     /translation="MPADLHATGTADRRAAVRNAGQTGGRRELPGGELRILLIEDSRP
                     LRERMIGMLTVPGVMQVTGTAETEREATALIDGNDYDALVVDVELREGSGITTIRHAR
                     RSYAARRQPLIIVLTNYALPTVRQRCIEVGADHFLDKMQQFLQVRPLLEAHYRHADS"
     gene            complement(33001..33639)
                     /locus_tag="PKN91_00685"
     CDS             complement(33001..33639)
                     /locus_tag="PKN91_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNS26612.1"
                     /translation="MPQVNVIIGDDHQVVRAGLRQIISSQPDLRVAAECRSGKDVIAA
                     LKEHACNVLLLDLSLPDMSGLDVLKHVRSHYEGIGVLVLSSYPEKQFGINVLRGGANG
                     FISKGVEESELLRAVRLVARGNRYVGPELADLLLSGIDGTGDQPRHRVLSEREFQIFV
                     KLAEGKTVTEIANKLFLSVKTVSTYRSRILDKMGMKTNADITYYAIKNSLLQ"
     gene            complement(33649..33846)
                     /locus_tag="PKN91_00690"
     CDS             complement(33649..33846)
                     /locus_tag="PKN91_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26613.1"
                     /translation="MSENSYSSLARVRAKYPVDAATSAVPGIMRWHIAAARGEIQRAA
                     AVTTSTWKRARRAVIGLLYDN"
     gene            33991..34671
                     /locus_tag="PKN91_00695"
     CDS             33991..34671
                     /locus_tag="PKN91_00695"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HNS26614.1"
                     /translation="MSSNVERAPGEKLTVQFEARKCIRSRHCVLGDPRVFVPNADGEP
                     NVEGEWIHPDAASVDAVVEIAHKCPSGAITYERIDGGPQEAPPPVNVVRVRENGPLAF
                     HAEMHFYGGGSALRAALCRCGASKSKPFCDTSHADAGFAASGEAPTRDSPALAVCNGP
                     LEVIPVENGPLHVKGNLEVVTGTGRTVERVTECWLCRCGGSGGKPFCDGTHRRIGFTA
                     AGLAPRRK"
     gene            complement(34687..35385)
                     /locus_tag="PKN91_00700"
     CDS             complement(34687..35385)
                     /locus_tag="PKN91_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNS26615.1"
                     /translation="MSGDEVVVDVPAASAGIVPAVDASVIWLHGLGADGHDFVPIVPE
                     LRLPPTVAVRFCFPHAPVRPVTLNGGLPMRAWYDIRELSTEGRQDEAGTRASAARVDQ
                     LVQREIDRGVAPARIVIAGFSQGGAVALHAALRYPARLGGVMALSTYLPLHASLEREA
                     SAANRDLPILMCHGQYDPVLPIGLGEASRDALQRLGYPVEWKTYPMQHEVCRAEIDDI
                     SGWLAARLAGGGHA"
     gene            complement(35382..36746)
                     /gene="hslU"
                     /locus_tag="PKN91_00705"
     CDS             complement(35382..36746)
                     /gene="hslU"
                     /locus_tag="PKN91_00705"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNS26616.1"
                     /translation="MSAEVKLPGGAALTPQQIVRELDKHIVGQGDAKRAVAIALRNRW
                     RRMQVDDDLRPEITPKNILMIGPTGCGKTEIARRLAKLANAPFVKIEATKFTEVGYVG
                     RDVDSIVKDLVDVAVKMAREQEVEKVRERALDAAEERVLDALLPKPRAMGFSTDEPPV
                     PRDAETRQKFRKMLRERQLDDREIEIELRAIPAGVDIMAPPGMEEMQQQLSSMFQNLA
                     GGRTHSRKVKIKEALKLLVDEEAAKLVNEEELKARAVANAEQNGIVFIDEIDKVTRRQ
                     ESFGADVSREGVQRDLLPLVEGSTVATRYGAVKTDHVLFIASGAFHLSKPSDLIPELQ
                     GRLPIRVELASLGVDDFVRILTEPDAALTTQYAALMETEGVRLVFAADGVRRIAEIAA
                     QVNERTENIGARRLHTVMERLLEEISFEATERHGSTVTVDAAYVDARLGNLAKDEDLS
                     RYIL"
     gene            complement(36746..37291)
                     /gene="hslV"
                     /locus_tag="PKN91_00710"
     CDS             complement(36746..37291)
                     /gene="hslV"
                     /locus_tag="PKN91_00710"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNS26617.1"
                     /translation="MSEVWNPHGTTILGVRRNGAVAVGGDGQVTLGNTVMKGNARKVR
                     PLGGGKVLAGFAGGTADAFTLFERFEGKLEKHGNNLTRAAIEIAKDWRSDRYLRRLEA
                     LLLVGDREKLLVISGNGDVIEPEHDLVAIGSGGAFAQAAARALVENTELDARTIVERA
                     LGIAADICIYTNRNFVIEELK"
     gene            complement(37304..38203)
                     /gene="xerC"
                     /locus_tag="PKN91_00715"
     CDS             complement(37304..38203)
                     /gene="xerC"
                     /locus_tag="PKN91_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNS26618.1"
                     /translation="MPSSIADWIARFRRHLATERRLSPHTDSNYARDLAALVRFCETE
                     RIADPARLDSQHVRLFAARSHAAGLAPRSVQRRLSAVRSFCTFLVREGAISTNPAHDV
                     RAPKAGKRLPGTLDADKVTQLLNVKATDPLALRDRALMELAYSSGLRLAEIVALDIGD
                     LDLADRTVRVLGKGAKTRILPVGRPALAALRSWLRERAALARAGETAVFVGRNGTRMG
                     PRAVQLRVAYWARRQGLGVHVHPHLFRHSFATHLLESSRDLRGVQELLGHADISTTQV
                     YTHLDFQHLARIYDSAHPRARKK"
     gene            complement(38184..38891)
                     /locus_tag="PKN91_00720"
     CDS             complement(38184..38891)
                     /locus_tag="PKN91_00720"
                     /inference="COORDINATES: protein motif:HMM:NF016243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HNS26619.1"
                     /translation="MTTREARGLPTETIDEESIARYLQLNPDFFERHQAILARMRLPH
                     ARSASTISLVERQVEVMRERQAELEGKLADFVRVARANDVLTGKIQRFTRRLLRATSR
                     QDMAAQIEASLREDFDAFHATLILVNGNERGFEGLPERFLRAVSADDPNFKSFEGLFA
                     SGKPRCGQVRDTQRQFLFGTDSTEIGSVALVPLGEHGSVGLLALGSHDRERFHPGMST
                     EFLARMGELIADAVVYR"
     gene            complement(38888..39754)
                     /gene="dapF"
                     /locus_tag="PKN91_00725"
     CDS             complement(38888..39754)
                     /gene="dapF"
                     /locus_tag="PKN91_00725"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNS26620.1"
                     /translation="MRVDFTKMHGLGNDFIVFDAPAGATPPSAAELRRLADRHTGIGF
                     DQALMLEPPRREGTATYYRIFNADGQEVEQCGNGARCIAALLAQRGKARDGELAMDSP
                     GGLIRARIEAGGLVSVDMGVPDFNPAALPFDASTEAHVYLLDVGGSEVEIGAVSMGNP
                     HAVLTVASAAAAPVDRLGPAIEHHPRFPKRVNVGFMEIVDRNHIILRVHERGTGETLA
                     CGTGACAAVAVGRRHGRLDERVSVRVAGGELTITWPGPGKNIWLTGPAEVSFTGSVDV
                     NGAHASRGAENP"
     gene            complement(39772..40197)
                     /locus_tag="PKN91_00730"
     CDS             complement(39772..40197)
                     /locus_tag="PKN91_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNS26621.1"
                     /translation="MFRNPDQAAIEQLLRTARTIAVVGLSTDPSRPSHDVARQLQRYG
                     YRIIPVNPAATTILGERAVPDLDHLADALPAGTRPDIVDVFRRPEHVAGLVDDCIRLG
                     MPALWLQDGVIDEAAASRAQAAGIFTVMDRCLFRDRAAL"
     gene            40285..41286
                     /locus_tag="PKN91_00735"
     CDS             40285..41286
                     /locus_tag="PKN91_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNS26622.1"
                     /translation="MRVRLLPSAREFDARPDEPVLAAALRGHFNLPHSCRSGHCSSCR
                     ARIVAGQVWYPGARPPGLTAEEEAAGLALLCQARAASDLVIEAREIRRVTDVEIKTLP
                     CRIETRERLAPDVLRLQLRLPAVEQFHFLPGQYLDVLLDGGRRRSFSIASPPHDAAQL
                     ELHVRRVPGGDFTGELFAHDAPGALLRIEGPIGQFVYVDAPGPVVFVAGGTGFAPVKS
                     MLRHALERGTPRDIHFYWGARTPVDLYEEALLYAWAARHPRFHYTGVLSEAPPDSAGH
                     ESGWVHEAVLAHHPDLSTFEVYAAGPPAMVEAIRARFPAQGLAPRRLHFDSFDYAPR"
     gene            complement(41299..41940)
                     /locus_tag="PKN91_00740"
     CDS             complement(41299..41940)
                     /locus_tag="PKN91_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26623.1"
                     /translation="MKVTAANAYGQAEQAFDDWLADHARSGWARAFEPATSTLRNRAL
                     GRVAAVLGTPAPSAAARRWVADTIQAWAPLEVLFPTRDYPDEPGVSGELAAHMTAIVE
                     REYAFMLTAARCTLVTEWLQADAQKLGLDLVLAEAMLAALPDGTDDCWVQQHIRMSLA
                     LAEYLHRQAIGMRQLLADAVAMSYTTSVALRRRQLHAACRSTPCLVATTRRYA"
     gene            complement(42006..42746)
                     /locus_tag="PKN91_00745"
     CDS             complement(42006..42746)
                     /locus_tag="PKN91_00745"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNS26624.1"
                     /translation="MKLKTLIVDDEPPARARLASLLAEIDDVQVVGEGASGREALELT
                     MDLSPDVVLIDVRMPDMDGLEAAQRIARMSEPPAVIFTTAYDEYAVNAFEAQAAGYLL
                     KPIRKDKLLAAIAACRRLTRPQMARAESATAAPAPRRSHVPVRRRDGIKLIPLGEIRC
                     FVADQKYTTVHHEGGEDLIEESLRLLEEEFADGYVRIHRSALVSVAHLERIERDPDGQ
                     YFVRLRGHADRLAVSRRMAGDLRERFRL"
     gene            complement(42721..43788)
                     /locus_tag="PKN91_00750"
     CDS             complement(42721..43788)
                     /locus_tag="PKN91_00750"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNS26625.1"
                     /translation="MSEAQPQHTHYLPDFCAGRTVLATVLVIVLTALIVTLGRDASVL
                     DFWTDLARTSFFLIWVGLAGQGLLCAARAQLARLSVAGSAAAVLGIVCAVVVIVSEVA
                     YRLGASDMLAPIEVGRLFPREHLPFILRNVVIAFIVTALALRYLYVTHEWRRNIEMQS
                     RARVHALQARIRPHFLFNSMNTIAALTRSHPALAEEAVQDLADLFRVSLAEKRDQITL
                     AEEIEVARTYERIERLRLGERLHVEWRVDAVPPDAIVPSLMIQPLLENAIYHGIEPRS
                     GGGTVKVTGEHANGLVTIVVRNPVPEGLVPRDGNRLALANIRERLSLMYGTRAAVKAG
                     RFDDEYIVTLRFPHEAQDAHR"
     gene            complement(43785..44669)
                     /locus_tag="PKN91_00755"
     CDS             complement(43785..44669)
                     /locus_tag="PKN91_00755"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNS26626.1"
                     /translation="MRAGFIGLGAMGQHMARNLLKAGHLVAVWNRTPATAEAFARDNG
                     VAAAASPAELAAAVDAVVLCVSADADVLEVVRRIAPALRPGALVIDCSTASAAAAREA
                     GAVLAARDVDFLDCPVSGGVEGARDGTLAVMCGGDAAAFERAGPLLRAMGRTITRFGA
                     LGAGQAAKATNQIMCAGIIRAVAEAMAFARAQDLPLDALVETLGKGAGSSWYFVNRAP
                     NMIRGSFPAGFKVRLHAKDLGICRAMAAECGVELPVVESMLAEYAELIRQGYGDEDIS
                     ADYRLKAALFERAARKDA"
     gene            complement(44759..45238)
                     /locus_tag="PKN91_00760"
     CDS             complement(44759..45238)
                     /locus_tag="PKN91_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNS26627.1"
                     /translation="MKAPPMRPLALIALAFLLAPLGAQADVYRWVDSRGEVHYSDRPV
                     EGAVLVKTASPRPPSPSDAAPSRVAATPYVAPPVSPEQATRAAGQAVAQDLARKRAEE
                     CTEAKARYEKSLQARRIFRTLENGEREYLTDAEADQVRVTNRAAMDAACGPAPRASR"
     gene            complement(45431..46747)
                     /gene="amt"
                     /locus_tag="PKN91_00765"
     CDS             complement(45431..46747)
                     /gene="amt"
                     /locus_tag="PKN91_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNS26628.1"
                     /translation="MRKLRTSLATALLLAVAPLIALGQELAAPVPDKADTTWMMLSTI
                     LVLLMVAPGLALFYGGMVRAKNMLSVLMQVFVVFSLVVVLWVLYGYSLAFTGEGAWIG
                     GFSRALLAGITPDSTAATFTDGVVIPELVFVAFQSTFAAITTCLIVGSFAERVRFAAV
                     LVFSVIWFTAAYLPIARMVWYGEGFMFQRGALDFAGGTVVHINAGIAGLVGSLVVGKR
                     LGYGTAPMAPHNLPLVMIGASLLWVGWFGFNVGSNLEANAFTGLVFINTLVATAAAIL
                     AWSAGEWIFRGKPTMLGAASGAVAGLVAITPSCGFLGVGGALVLGLIAGCACLWAVAR
                     LKVWAGYDDSLDVFGVHAVGGIVGAILTGVLVSPTFGGTGIDGYSMGAQVWTQLLGVL
                     ITIVWSGIVAFIAYKIADLTVGLRVTEEQEREGLDTTYHGERAYQG"
     gene            complement(46776..47114)
                     /locus_tag="PKN91_00770"
     CDS             complement(46776..47114)
                     /locus_tag="PKN91_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNS26629.1"
                     /translation="MKMITALIRSHKLDELQLAVARLGVQGLTVTEVMGHGRQRGHLE
                     HYRSAGYRVEVVPKTRVEIAVGEDIVEPVVEAIANVARTGKVGDGKVFVAPLEEAVRI
                     RTGETGAAAT"
     gene            47318..47575
                     /locus_tag="PKN91_00775"
     CDS             47318..47575
                     /locus_tag="PKN91_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HNS26630.1"
                     /translation="MERIPIDELARRLLESLPAGVRTLQRDLETNFRAVLRANLARLD
                     LVARDEFDAQARVLERTRARLEALEAQVAALERARDSGASD"
     gene            47562..>49017
                     /locus_tag="PKN91_00780"
     CDS             47562..>49017
                     /locus_tag="PKN91_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNS26631.1"
                     /translation="MRATEVTQVARVASRAQVGLTAPVVDVEVHLGPGLPNFTIVGLP
                     AAAVRESRERVRSALACCGFELPAGRITVNLAPADLPKEGGRFDLSIAVGILIASRQL
                     RVAQALGGCELYGELGLAGELRPVPGLLPAAAHAAAAGHQVIVPAANAWEAAMAAPQV
                     AGAAHLSEVCAYLAGDAAACARTAHARPASACRAPREYPDLRDVRGQRAAKRALTIAA
                     AGSHSLLLVGPPGSGKTMLAQRLPGLLPPLDTAEALEVATIASVGGRGFDHGNWGCRP
                     FRSPHHTASAHAIVGGGPRARPGEVTLAHLGVLFLDELPEFDRRVLEGLREPLENGSV
                     AISRAALDVHYPARFQFVAAMNPCPCGYHGDESGRCCCSPTAIAKYRGRLSGPLLDRL
                     DLRVEVPRVADPLCDGPPADEAASAALSRCVSAARARQMERAGKLNAWLTVRELAGHC
                     ALAPAGHALLVASSRTLMLSARSQHRILRVARTIA"
BASE COUNT         7545 a        16545 c        16931 g         7996 t
ORIGIN      
        1 cccgccaatt ccgccaacgc cgcctcccag gcgcgccatg cacgtccacc gcactgagca
       61 tctgggccac gaccacgtct atctcggcga cgatcacgag cgcaacgagc gccgcacgtg
      121 gtgggtcatc gggctgacgc tcgtgatgat ggtcggcgaa gtcgtcgccg gcatgctgtt
      181 cggttcgatg gcgctgctcg ccgacggctg gcacatggcg acccatgccg gtgcgctcag
      241 catcaccgcg ctcgcctatg cctatgcgcg ccgccacgca cgcaatgcgc gcttcacctt
      301 tggcaccggc aaggtcggcg acctcgcagg cttcgccagc gcgctgatcc tcgccgtgat
      361 cgcgttgctg atcgccgtcg aatccctgca gcgatttctg gccccggtac cgatcgcgtt
      421 caccgaagcg accgccgttg cggcgctggg cctcgcggtg aatctcgtca gtgcgctgct
      481 cctccgtggc ggccactcgc acgcgggcaa tgggcgccgc ggccacgacc acgaccatga
      541 ccacgaacac ggccacacgg ccagtggcgc ggacctcaac ctgcgcgcgg cctacctgca
      601 tgtcatcgcc gatgcgttga cgtcggtcgc cgcgatcctt gcactggtac tcgggcgctg
      661 gctcggctgg gtgtggctcg acccggtgat cggcgtcgcg ggcgccgtgc tgatcgggcg
      721 ctggtcgctc ggcctgatgc gcgccacggg cgaggtcctg ctcgatgccg cggacgacac
      781 cgcgctcgct caggctgtgc gcgaggcgat cgaagccggc gacgagaccg tcaccgacct
      841 gcacctgtgg cgcgttggtc cgggacgcca cgccgcgatc gtgtcgctgg tggccgcgca
      901 gccgctcgcg ccccaggaat acaaggcccg ccttgcgcac ctgccacagg tcgtgcacgt
      961 gtcgatcgaa gcccatcgct gcccggagca cccgcaatga acctgctggc actcgcctgc
     1021 ctcgaggcgg ccgtgttttt caatgcgaac ctgcataccg gtgccgaacc ggtggccggc
     1081 gcggacgcca tcctcgtgca ggatgcgcgc atcagtttca tcgggccgac ggaggaagca
     1141 cgtcgccgcg cacccgaggg tgcgcacttc gtggatctcg ccggcgcgac cgtcgtgccc
     1201 gggttcaccg atgcgcatgc ccacctcgcg ggcatcggtc tgcgcgagat ggctttcgac
     1261 ctcgacggca cggcgagcct cgccgaactg caggccaggc tcgccgcacg ggctgcatcg
     1321 agcccggacg actggatcac ggggcgcggc tggatcgaat cgcgctggga gcccgcgcgg
     1381 tttccgacgc gtgcggcgct ggatgccgtc gtcgccgatc gcccggtgct gctcgagcgc
     1441 gccgacggcc atgccgcagt ggccaacagt cgcgcacttg cgctcgcggg tatcgacgca
     1501 tcgacgccgg atcctgcggg cggccggatc ctgcgtgatg cgcacggtga gcccacgggc
     1561 atgctgatcg atgcggcgac gcggctcgtc gagcggctcg tgccgccacc ctctgaagcc
     1621 gacgcgttgc gcgccctcga gctgggcgcg gcgcgcagcg tgcggctcgg ctggacgcag
     1681 ctgcaggatg cgggcaccga tctcgccggg atcacgcgcc tgtgccggct ctacgacgag
     1741 ggcagggtca agttgcggct ctattccgcg atcggcgggc cgggcagcga tgcggacgcg
     1801 ctgctcgccg ggcgcatcgc gcgccatccc tgcggcgagc gtctcacggt gcgtgcgatc
     1861 aagctctaca tggacggtgc gctcggctcg cgcggcgccg cgctgctcgc gccttacagc
     1921 gatgcgcggg acagcacggg cctgttgctc aacacggaga cgcagctgct gccgattctc
     1981 gagcaggcgc tgcgctcggg gctacagatc gagacgcacg cgatcggcga tcgcggcaac
     2041 cgcatggtgc tcgatctcta cgagcgcgcc ttcgacgccg tgcccgtcgc ggagcgctcg
     2101 ctcgccgaac cgcgctggcg catcgagcat gcgcaggtgc tggaccgcgc ggacctgccg
     2161 cgcttctcgg ccctcggcgt gatcgcctcg atgcagccct cgcatgcgat cagcgacctg
     2221 tacttcgcgc cggcgcgcct cggtgcgcag cgcctcgccg gcgcgtacgc ctggcaaagc
     2281 ctgctcgcga gcggtgcgat cgtggccgcc ggctccgacg cgccggtcga gcgcggcgat
     2341 cccgtgcagg agttctacgc cgccgtggtg cggcgctcgc ccgagggtta cgcgggcgag
     2401 gactggcatc gcgagcagcg cgtcacgcgc gagcaggcac tgcgcatgct cactctcgcg
     2461 ccggcgcaag ccgcattcga ggagcgcgag cgcggcacgc tggaggtggg caagctcgcc
     2521 gacttcactg tgctgtcgca ggacctgctg gcggtgccgg acgaggcgat tctcgcgaca
     2581 cgcgtgctga tgacggtaat cggtggggag atcgcctacc gcgcggactt gacagctgtc
     2641 acgactgaat gagtttcatt cccgttttgt gcgctgcgcc aagggtatca ttggcgaagc
     2701 ggtttacgag accgcttcac tggagtcccc ccgcaaatga acaagactga acgactgacc
     2761 cggcggcaac tgatcggcgt gacggtgacg gctgcggccg gcctgtcggg cgctgccgcg
     2821 tttgcgcaag caccggccgg cgccgcgagt tttccgaagc tcgacgagaa ggagccgacc
     2881 gccgttgcgc tcaagtacgt gcatgacgcc aagctggttg atccgaagaa gagcccgaac
     2941 tacaagcccg gccagaagtg cgccaactgc ctgcagatca ccggcaagga cggcgacgcc
     3001 tggcgtccct gcaacatctt cccgaagaag ctcgttgccg cggacggctg gtgcagcgtc
     3061 tgggtgcaca agcccggcac cgccatctga gcctgcgcgc gccgtgcgtc gccacgctcc
     3121 tgtgcctgat tgcggcgcag gtcgtggcgg ccaccgcggg cgcgttctcc atcactgacg
     3181 ataccggcca gccggtcgcg ctcacacggc cggctgcccg catcgtcgcg ctcgcaccgg
     3241 gcgcgaccgc gctgctgttt gcggcgggcg ccggcgatcg catcgtggcc acgatcgagt
     3301 acgccgacga acccgatgcg gcgcggcgac tgccgcgcat cggcgatgtg caggcgatcg
     3361 acctggaacg cctcgtcgca ttgcggccgg atgtggtcgt cgtcaccgaa gggatcacct
     3421 cgccgctggt catcgaacgc gtccgcggcc tgcacctgtc ggtctatgcg acccgctatt
     3481 cgcggctcga cgacattgcg cccagcgtcg cgaggctcgg gcgcctcgcc gacacggcag
     3541 acgtggccga agccgccgcc gcgcggctcg ccggcacgct cgcggcgctg cgcaggcagt
     3601 acgccggtcg cgggccgctc gaagtcctgt accaggtctg gaaccggccg atctattcga
     3661 tcggcggccc gcacatcgtg accgatgcgc tcgcgacctg cggcgcgcac aatgccttcg
     3721 cggaccagcg tgtcgccgca ccggcgctcg gcatcgaggc ggtgatcgcg cgcaatcccg
     3781 acgtcatcgt cgtgtcggca ccccgcgccg tggcgagcgc ttgggtcgag gagtggcgga
     3841 agttcccgcg cctcgccgcg acggcgaacg atcgcctgta tgtgttcgat gatccgcgcc
     3901 tcgatcgcat gggaccggcg gcgatcgagg ccaccgcaaa cctgtgtcgc atcctcgacg
     3961 ccgcgcgcta gtcgcggaag ttctcgaact gcagcggccg gtcgaacggc gacttcctta
     4021 gaagcgccat cgtggcctgc aggtcgtcgc gttgcttgcc ggtgacgcgc accttttcgc
     4081 cctggatcgc gcactgcacc ttgagcttcg agtccttgac gagcttcgtg atcttgcggc
     4141 ccgtctcgtg gtcgatgccg tggcgcagct ccacctgctg gcgcgccgcg gcgaggttcg
     4201 tctccggatc cttgatgtcg agtgagacga tatcgatgcc gcgcttgccg agcttgatgc
     4261 gcaggatgtc gagcatctgc ttcagctgga actcgacctg cgcctgcagc gtgatggtgt
     4321 ggccctcgag ttcgtacttc gcaccggtgt cgcgaaagtc gaagcgctgc gcgagctcgc
     4381 gattcgcctg gtccacggcg ttcgtcagct cgtgcaggtc gatctcggag acgatgtcga
     4441 aggagggcat cagccttccg ccagcagttg ttcgagcagc gcgtcgaact gcgccgtcag
     4501 cgcgtcgtag tgcgcgccgg tcgcgggccc cgaaaagccg gtgtgcacat ggcgcacgcg
     4561 gcccgagcgg tcgaggaaca gcgtggtcgg gaaggcgtag atcgcgtcga gctgcggcag
     4621 cttcttcgcg acgtcgtccc tgtcggaaac accggcgatc aatgtcgtgt actggatgcc
     4681 gaggtccgcg cggaagaggc gcgtggcggc cgccgccgcc tcgaagtcgc cgaagtgctc
     4741 gaacatgagc gagatcacct cgaggccgcg ggcgcgattg cgctggtacc agggcgcgag
     4801 gaacatcgct tcgtcgtggc agttcgggca ccagctgccg gccagcgtga tcacgaccac
     4861 cttgtccgca aagcgcgggt ccgagagcga caccggctgg ccgtcgagat cggggaacgt
     4921 gaactcgagg cgttcggccg cattgcgcac ggcggtggcg ggtgcgcctt cgagtgtcgc
     4981 tgcctcgtcg cggcgcgcgc tgaacgcctg ctcccaggcg agccccgacc agaacgtgcc
     5041 ggcgagcgtg tcatccgccg caagctgcgc gcggtacagg taggcgtgcg cgccgtcgaa
     5101 cgtcgacagg tacagctcgt tgccgcgcat ctcgcccgcg agataccgat gatcacccgt
     5161 gggcgtcagg atcgtaccgg tcacggcggc gccttcctgc cggaactcgg caacgccggg
     5221 cgaggtcttg ccgtccgcct cggcgaaggt cacggcccag cgcccggaca cgctaccggc
     5281 gctcgcggcc ggcgtggcga agaagcgatg cgtgacgccg cgctcggcga tgaacgggat
     5341 gacctggttc gcgccgccgc gcttgaccag cagcagttcg ccgcgcagcg cggcgccttc
     5401 ggctttcgcg ctgatgcggt tctcgtagcc cggcatgcgc gcctcgagcg tgtccccggc
     5461 aagcgtcaca tcctcgacgg cgagccgctc ggcgccgttc tgcaggtaga tgaggttgcg
     5521 atcgccgagg cgcacgagtt cgaagccgaa cggcagctcg ccgcccggca actgcagcgt
     5581 cgcgcggtac tgaccggcca agacggagtc ctcgacgggc ggcgcatcag ccggcgggcg
     5641 cgcgcaggcc gcaagtacga cggatgccgc gatgaaaagc gtagtgcgtg tcataccgat
     5701 agtggcgccg ttggggcgcc ctcccaggcc ggcgcaggaa agccgtccgc accgcgccgc
     5761 cacggaaccc acaccagtga gatctcgcca atggcaatgt cgctcttgcg cggcgcgatg
     5821 gatttgtgtt cgagcgcgag cgtgctggcg tcgaagtcgg tcgccgccac ggcgagggct
     5881 gcttcgcatt cggcgtcgag gtccgcgagg cgctgcgtga gcacggccag gttgtcctcg
     5941 gcgcgtgcga cgtcggcttg ttcgcggccc acgcgcgagg cggtgcgcgc agccgtgccg
     6001 gcgcgaccca cggtcgaggc gctgagcgcc ttgcgcccga agagtgcgcc gagcaccgcc
     6061 gagcccaccg acaccgccgt ctgcgtgcga cgctcggaca actggtcgcg ctctcggccg
     6121 acgcgctcct cggcgcgccg cacctggtcc gcaaggctcg tacgcttcgc ggcgtagcgg
     6181 tcgcgtatct tctccacggc cgcgtcgcgc cgctcgcgcg cggcatgcgc gaggcgcgcg
     6241 cggaagtcgc cttcgctctc gccgggctgc gacgtcatcg ccagcgtctt gctggagtag
     6301 atcccggcgc gcgccgattc gtacaggctg gcggcgaggg ccttgcccca ccgggcgtaa
     6361 ttctgcgcac gcagcgctgc agcgggcagc gtcgcaaact gcgcatccgc tgcgggctgg
     6421 tctgcgagta cgccgggcgc tgcaccctcg ccggcgggtg gccattccac ctcatcgccc
     6481 gagtcgctga atggcgcgag cagcgtgacg gtgcgccatt cgtcgacttt cgagcgggcg
     6541 tcgacgaagt gcagcttcgc cgtgccgagg atcatgggcc gatactcgat cgggccggcg
     6601 ccgcgcaggg cagcggcaaa gtactcgcgc acgtcggcgg gcacggcggg gcgggcgctg
     6661 gatgccgtcg ctccagacgc cgcggccgaa gatgtcgcgg ccattgccgc cggtgcggcc
     6721 gatgctccgg ccggtgtggc cggcgccacc gcagtgctgg ggtcgcgctt gcgatcggcc
     6781 atcagccgcg agatctctgg cccggtgagc ggtccgcgca ggtaggacag cgcccagcgc
     6841 gactgcatca gctgcggcgc atcgtcgtgc acgttacgca tcaggaagac gcgctgcgtg
     6901 aggctgccca tcaatctgtc gagcgtcgcg cggctgcccg ccgccccgcc cggcagcgcg
     6961 ctctcgaggc cgtcgagcac gcgctgcttg tcgcggtcgg tctgcaggcg accgatgaac
     7021 caggtgcccg tgttgccgag ccccttgtag tcgagatcga ccgggttctg ggtcgcgagc
     7081 acgcagccga caccgaaggc gcgtgcctgc ttcagcaggg tgagcatcgg tggtttcgag
     7141 ggcggattcg ccgtcggcgg gaagtagccg aagatctcgt ccatgtagag cagggcgcgc
     7201 agcgaggagg ttcccgactg gcggcgcatc cagccgatca gctcgttcag cacgagtgtg
     7261 acgacgaaca tccgctcggg atcggacagg tgagcgatcg agatgatgct gatgcgcggc
     7321 ttgccctctg gcgtatacag cagacgctgc gcgtcgagcg gatcgccttc gagccatgcc
     7381 gcgaaccctg gcgaggcgag caggttgttg agcgccatcg cgagcgcggt gcgctccttc
     7441 gccggataga aagtctcgag atcgagcgcc ccgaccttgt ccatcggcgg cttctgcacc
     7501 gcggcgatca ggcgagcgag atcgagcccg tcacccgcac gccaggcctg ctcgatgatg
     7561 ctcgagagca ggatgtgctc gcggctctgc agcggatcgg cgtcgcgtcc gacgaggctc
     7621 agcagtccgc cgaccacgcc gctcacacgc tcgcgcagtg aggtgacatc ggcacgcagc
     7681 gcctcggccg gcgcatcgag cgagcgcagt accgacaacg gcaggccggt gttggcaccc
     7741 ggcgtgtaga tcgcgagatc gacccgcgcg cgcagcgccg caatgcgatc gggcgtctgg
     7801 ttccagtcgg cgaggccctt gcgccagttg ttcgcgacca ccttggcgta gtccggcacc
     7861 gactggccct tgcgcgccgc ctcggccggg tcgatccacg gctcgaagtc ggccggtgcc
     7921 agctgcggga aggtcagcag caggttcgcg agatcgccct tcgggtcgat gcagatcgcg
     7981 ggcacgccat cgatgccggc ttcctccagc agcgcgaggc acaagccggt cttgccgctg
     8041 ccggtcatgc cgacgcagac cgcgtgagtc gtcagatcgc gcgagtcgta cagcagcggc
     8101 tcgggcaggg ccgccccggc ctgcggatcg tagggccgtc cgagatagaa gacgccgagc
     8161 ttctcgtagt cgccggccat ctacttcgcc ttgggcaatc cggcctgcag gcgtttcctg
     8221 tgcttgcgcg caagttcgat gagcctcggc gtgagcccga tgtcctcgta gatcggatcg
     8281 ccctggtcgt cggtcgagat caccttgctg ccgcgctcgt agggcagcgc cgagaccatt
     8341 tcatcgagcg cgaggccgag cagctcggtg atcatctgct cgcgggtgcg acccgggaac
     8401 agttccgcga gcgcatcgag ccgggcggcg tcatcgagcg gcagccgcac cgcgtactcg
     8461 gtcaccgtgc gcggcgacat gccgccggtg cgccaggcgt cgaggagttc cctgaagccc
     8521 acggcctact ccagattgcg ggtggtgcgc cgattgtacc tttgcggcgg cgcgtaatcc
     8581 gcaccgcggc gcgcggccgg taagtgtttc ccgcaggtcg cgccccggcg ggcaaccggc
     8641 attgacgctg cgacggccgc tcccccatac tgagaacaga tgaaatcccg cgaattcacg
     8701 gctcattgct ggtggtggcg acactcctga cggagtgggc gccgacgtga ccgactagtc
     8761 ccccggattt ctcaggtttc gatacgcaag acccatctcc gtcgctgacg gcgatgggtt
     8821 tttgttttgc gcggcttgcg gcagggaatg tggatgcgga agactttcga catagcaggt
     8881 gacctcgata cgccggtctc ggcgttcatg aagctcggcg cattccggcc gcgtttcctg
     8941 ctcgagagtg tcgagggcgg cgagcgcctt ggccgatatt ccttcatcgg cttcggcgac
     9001 agcctggagt accggctcga atccgatgcg ctgtacgtcg acggcgtgcg cacgccggcg
     9061 cccgcggatc gcgatgccct gctcgccagc ctgcgcgcgg cgctggcgcg cgccgcgcgg
     9121 ccacagcccg agatcgccca cgtgccgctc gccggtgggc tcgtgggcta tgccgcctac
     9181 gacgtcgtgc ggtatttcga acgcctgccg gagcatgcgc agccggtcgc cgggacaccg
     9241 ctgatgcatt acgtcgcgcc gcgctcgatg ctggtgttcg atcacctgac gcgcggcatc
     9301 gcggtgctgc acgcgggctc ggccggtgag cgtgaatcgc tgcggcggga aatcgtgcgc
     9361 gcactgcgcg gcggattgcc gaacgggcag gcgcgcggcc gtcacagcga gcccgtgccg
     9421 tcgctgacgc gcgaggctta catcgagggc gtgcggcgcg ctaaagaggc gatcaccgcg
     9481 ggcgatgtgt accagctcgt gctgtcggcc cggttctccg gccggcacga tctcgatccg
     9541 ttccaggtct accgcgcact gcgtttgatc aatccctcgc cgtacctgta ctacagccgg
     9601 ctcggtgagc acacggtggt cggctcctca cccgaggcgc tggtcaagct ctccgcgggt
     9661 gtcgccgagc tgcggccgat cgccggcacg cgaccccgcg acgacgaccc gcagcgggac
     9721 gcggccaacg aggcggcgct gcgcgccgac ccgaaggaga acgccgagca cgtgatgctg
     9781 gtcgatctcg cgcgcaacga cctgggccgc gtggcgagcg ccggcagcgt gcgcgtcgaa
     9841 ccgtaccgct cgatcgaacg ctacagccat gtgatgcaca tggtgagcgg cgtgcacggc
     9901 acgctggcgc cgggacgcga tgccttcgac ctgttcgcgg cggcatttcc tgccggcacg
     9961 ctggttggtg cgccgaaggt gcgcgcgatg cagctgatcg acgaactcga gccggtcagg
    10021 cgcggcctct acggcggaac gatcggctac ttcggcgcgc agggcgacat ggaccaggcc
    10081 atcacgatcc gcaccctcgt attcaacggc gacgagtaca gcttccaggc cggcgccggc
    10141 atcgtcgcgg acagtgatcc ggacgccgaa tacgacgagg ttcgcgccaa gggcgcggcg
    10201 ctcgtgcgtg cactcgcgct ggcggaggag gggctgtgag cgcgcgcctg ctgctgatcg
    10261 acaactacga ctcgttcacc tacaacctcg tgcaggcctt catggtcctc ggcgcagagg
    10321 tactcgtcta tcgcaacgac gcgatcgacg tcgaggccgc gcgccggctc gagccgacac
    10381 acctgtgcat ctcgccgggc ccgggcacgc cgcgggacgc cggcgtgtcg atcgcgatga
    10441 tccgcgcctt cgccggacag atcccggtgt tcggtgtgtg cctcggtcac caggccatcg
    10501 tcgaggcctt cggtggcgag atcgtgcggg ccggacggct gatgcacggc aagacctcga
    10561 cgatcgaaca tgacggccgc ggcctgttcg ccggcctgcc gcagccgtgc acggtcggcc
    10621 gctatcattc gctgatcgcc gcgcccgacc ggctccccgc cgagctcgag gtgagtgcgc
    10681 gtaccgcgca gggcgagatc atggccgtgc gccaccgcaa gtttgatgtc gagggcgtgc
    10741 agttccatcc cgagagcgtg ctgacgccgg acggcccaca gctgatggcc aatttcctga
    10801 agacccgcca tgcgtgaact gctgcagaga ctgatcgacc gcgaagacct cgacgaggcg
    10861 gctgccggcg cgctgctgcg cggcctggcc gacaccgcaa cgccgcccgc gctcgccggc
    10921 gctgcgctcg ccgcgctgca ggcgaagggc gtggcggcca gcgagttgcg cggcctggcg
    10981 ctcgcgatgc gctcgctcgc gcgccggcct gagatttccc cgggcgcgcc gacgctcgac
    11041 atcgtcggta ccggaggcga ccgttcgggc agcgtcaatc tgtcgacggg cagtgcactg
    11101 ctcgcggccg cctgcggcct gcgcgtcgtc aagcacggca accgttcggt ctcgagccgc
    11161 accggcagcg ccgacgtgct cgaggcgctc ggcctcgccc tgcccctcga tgagcaggcg
    11221 gcgggcagcc tgctcgacca gtgcggtttc acgttcctgt tcgcgcccta ctatcacccg
    11281 gcgatgaagg ccatcgcgcc ggtgcgtgcg gcgctcggtg tgcgcaccgc gttcaatatc
    11341 ctggggccgc tggtgaaccc ggccgaaccg gcgttccacc tgatcggcgc gtacagcgcg
    11401 ccggtcgcgc ggctgatggc cgatgcgctc gccggcatgc cgatcgagcg cgcattcgtc
    11461 gtgcatggcg cggagggctg ggacgagccg acgccggtcg gcgagttcat gttgatcgac
    11521 gtgcgtccgg gcagcatcgt cgagacgatc cgcgatccgc gcgaggccgg catggcgcgc
    11581 tgtgcggcgg ccgcgctggt cggcgaggat gcgtcattca acgccgcggc gctgcagcgc
    11641 acgctgcagg gcgaggagcg cggtgcgctg cgcgatgcgc tgacactggg cgcgggactc
    11701 gcgctcgaac tcgcgggcaa ggcgcgcagc ctggaggagg gcatcgccgc cgcggcggcc
    11761 gccatcgacg ccggccgcgc cggcggcgtg ctcgcggcac tcggggcatc gcgccgatga
    11821 gcgatttcct cgcgacgatg gccgccgcga gccgcgcccg tgtgcacgcg gcgctcgccg
    11881 cctgccctct cgatgcgatg gccgcgcgag cgcgcaactc gccgccgcca ccgccgctcg
    11941 tgctgtcgcc cgggcgcttc gacatcatcg ccgagatgaa gctgcgctcg ccggccgccg
    12001 gtgcgctgcg cgcggcatcg gccgacgata tcgctgcgcg cgtgcgcgat tacgcgcggg
    12061 cgggcgcggc ggcggtgtcg gtgctcaccg agccgagccg cttcgacggt gcactcgaac
    12121 atctcgcgca ggccgcgcac gcgctcgcgc cggcacgggt gccggcgatg cgcaaggatt
    12181 tcctcgtcga tccctaccag gtcatggagg cgcgcgtcgc cggcgccggt ggcgtgctcg
    12241 tgatcctgcg catgttgccg cgcaccgata tcgaggcgtt gctggctgcc gccgcgtcgc
    12301 tcgaactgtt cgcgctgctc gaggcgttcg atgaggcgga catcgaactc gcgcacgagc
    12361 tggcgcgcca cgccgggggc aggctgctgg tgggcctcaa ctgccgcgat ctcgcgacgc
    12421 tccaggtcgt gccaggcagg ctcgaagcgc tcgccccgcg cctgccggcg catctgccgc
    12481 gcgtggcgga gagcggcctc gattcggcgc aggatgcggc gcgcatcgcc gcggcaggct
    12541 acgatctcgc gctcgtcggg agcgcactga tgaagcggga cgcacccggt acgctgctgg
    12601 ccgatatgct cgcggccggg cgcgcagccc gcgggtccgt gccgtgatgt gggtgaagat
    12661 ctgcggcatg acgacgcccg cggcggtcga cgcggcagtc gccgcgggtg ccgatgccat
    12721 cggtttcgtg ttcgcgccct cggtgcgacg ggtcgcaccc gcgcatgccg caatgcttgc
    12781 ggccccggcg cggggtcgcg cgcgatgcgt ggccgtgatg cagcatccga gccaggccga
    12841 agtgacggcg atgctggccg agtttgcgcc cgacgtgctg cagacggaca gcgaagactt
    12901 cgcggcgctc gacctgcctg cctcgctcga gcgcctgccg gtgctgcgtg ccggtcgcga
    12961 actgcccacg cagctgccgc gccgcgtact gtatgagggc gcaaggagcg gcgcgggcga
    13021 agtcgccgac tggacccggg ccgcgcagct gtgcgtgcgc aacgtcgaag tcgtgctggc
    13081 gggcgggttg acgccggaga atgtcgacag tgcggtgcgc gcggtgcgcc cgttcggcgt
    13141 cgatgtgtcg agtggcgtcg agagcgcacc gggcgtcaag agcgcggcga ggatcgcagg
    13201 attcgtggcg gcggcgcgag ccgccggccg ggagggctga catgacatcg ttgagtacgc
    13261 aggagcaggc gcaactgctc gctgattcca tcgccgggaa atttccggat gagcgtgggc
    13321 gtttcggccc gtacggcggc cgctacgtgc ccgagaccct gatccccgcg atcgagcggc
    13381 tcgaggcggg cctgcgtgca cacctgcatg cgccggattt ccagcacgaa ctggccgatg
    13441 agctgcgcag ctgggtcggc cggccgaccg cgctcacgca cgcgcgccgc ctcggcgcgc
    13501 gctggggcgc cgaggtatgg ctcaagcgcg aggacctcgc gcacaccggc gcgcacaaga
    13561 tcaacaacgc gatcggccag gcgctgctgg ccaagcgcct cggcgccaag cgcgtcgtcg
    13621 cggagacggg cgcgggccag cacggcgtgg cgagcgccgc ggcgtgcgcg cgcgtgggcc
    13681 tgccgtgcac ggtgtacatg ggcgccgtcg acatggaacg ccaggcgccg aacgtcgggc
    13741 gcatgcgcct gctcggcgcc caggtcgtgc cggtgacgga aggcgaccag acgctgcgcg
    13801 cggcggtcga cgaggcgctg cgcgactggg tcgccgatcc gctcgaaacc tattacctgc
    13861 tcggctcggc gatcgggccg catccctatc cctatctcgt gcgcgaactg cagacggtca
    13921 tcggtcgcga ggcgcgcgcg cagatcctcg agcaggccgg cggcctgccg gacgcgctga
    13981 tcgcctgcgt cggcggcggt tcgaacgcga tcgggatttt caagccgttc atcgccgatg
    14041 cctccgtggc gatcctcggt atcgaggccg ggggccgcgg cacggcgctc ggggagaact
    14101 ccgcgacgct cgcgctcggt cggcccggcg tgctgcaggg cgcctattcg gtgctgctgc
    14161 aggacgagca cggccagatc caggagacgc attcggtttc ggcgggtctc gattatcccg
    14221 gcgtcgggcc cgagcacgcg ctgctgcagg ccgcggggcg tgtcagctac gagtcggcga
    14281 gcgacgacga ggcgctcgcc gcggtcaagg agtgctgcga ggccgagggc atcctgccgg
    14341 cgatcgagag cgcgcatgcg ctcgccggtg cgcgtcgctg ggcggcggca catcccggca
    14401 aacgcatcct gatcggcctc tcgggtcgcg gcgacaagga catgccgacc ctgcagcgca
    14461 cgctgatcgc gcagctcgag gcagccgcgc acggggcgcg gtcgtgacgg cgccgcagtc
    14521 cctggcgccg cacgagcgca tcagcgccgc gatccgcgcg gcggctccac atggtccggc
    14581 gctcgtcgca ttcatgaccg ccggctaccc gaagcgcgag cgcttccgcg agcagcttgc
    14641 ggcggtcgcg ggtgccgccg acgtcgtcga gatcggcgtg ccgttcaccg atccgatggc
    14701 ggacggcgtg accatccagc gctcgagccg cgaggcgctc gcacaggggg tcacgctcga
    14761 gtggatactc gaggaactcg ccgccatgcc ggccctgcgg gcgccgctgc tgctgatgag
    14821 ttacctcaat ccgctgctgt cattcggttt cgagaagctc gcgcaggctg cggagcgcgc
    14881 gcgcgtcgcg ggcttcatcg tgcccgatct gccgttcgac gagggtggcg agctggccga
    14941 ggcgctcgcc gcgcgcggca tcgcgctcgt gcagatggcg acgccggtca gcacgcccga
    15001 gcgcctgcgc agggtctgcg gcgcaagccg cggtttcgtc tatgccgtca cgatgaccgg
    15061 aacgaccggc aggagtgccg ccgtgcccga cgacgtgctc ggctacctgc agcgcgtgcg
    15121 ggccgtgtcg caggtgccgg tatgcgccgg attcggcatc cgcacgcgcg agcaggtcgc
    15181 gcggctcgcc ggacatgtcg acggtgtggt cgtcgggtcc gcactcgtcg aggtgctcga
    15241 gcgcggcgag gatcccgggg cctggctgcg cggactgcgt caggacggcg agtgacacat
    15301 cagcccgacc cgcgcccgac tctcgggctc gcggcactgc tcgccttcct gtcgatggtg
    15361 agcccgttcg cgctcgatac tttctttccg tcattcccgg cgattgcgca gcagttctcg
    15421 ctctcggcgc tgcagttgca gcagacgctc accgcctacc tgctgcccta tggcctcacg
    15481 atgctgctgc acgggccgct gtcggacgcc tacggacgcc ggcgcgtgat cctcatcggc
    15541 gtgagcctgt tcgcgctcgc ttcgctcgcc tgtgcattcg cgccgagctt cggctggctg
    15601 ctcgcgtttc gcgcggtgca gggcgccacg gccggcgccg gcatggtcgt ctcccgcgcc
    15661 atcgtgcgtg acctgtactc cggccacgag gcgcagcggc tgatgtcgct gatgacgatg
    15721 ctgttcggtt ttgcgccggc gatcgcgccg gtgatcggcg gctgggtgca tgtcgccttc
    15781 gggtggcgcg ccgtgttcat cttcctcgtg ttgctcggcg ccacgatcac attcgtcgcc
    15841 tggtggcgct tgccggagac gcatccgcca gagcggcgtt tcgccttcca gtttcgctcg
    15901 ctgatcacga tttcctggcg catcgtcagc caccgcgagt ttctgttgct cgcgctgtcg
    15961 accgcgctgc acttcgtttc ggtggtcgcc tatatcggcg ccgcccccgc catcgtgctc
    16021 gagcagtggg ggctcaccga aacgcagttc gcgatgctgt tcgtaccgct catcggtggc
    16081 ttcgtcgccg gtgcgttcac ttcgggacgc atggcgggcc gcctgagccc gcacgcacag
    16141 acccgcctcg gcttcctggt ctcggccatc ggcgggctgc tctcgacgct cgcactctgg
    16201 ctgttctctc cggtgccgat cattgtgcag caggtgctca tattcactgc agcctacggt
    16261 gtgcagatcg tgagtccgat catcacgttg cgcctgctcg acctgtaccc ggacacccgt
    16321 ggcgcggtgt cgtccgtgca gtcattcgtg tcgatcatgg tcgcggcctt cacgatgggg
    16381 gtggtggtgc cggcgctgca cggctccctg ctgctgctgg cgctcggctc gctggtcggc
    16441 gcgctggtcg gttggtcgct gtggcgcatg gcggacgcgg cgccggcaag gtagattcgg
    16501 caaagggggg cttcccggag gtggcgggag gatggatacg gtcgacttca ttacggtgcc
    16561 gagtcgtttt cgcggcccgg atcactcggc aaacggcggc tatttcgcgg ggctggtctc
    16621 ggcgcgctcg gcctcgcctg tgaccgtgcg gctgctctcg ccgccgcccc tcgatgagcc
    16681 gatgctgata cagcgcgaag gcgcgacact gcgcgtcacc cacgcggaga ccatcgtcgc
    16741 cgaggcgcgc gccatcgacg gtggcctgcc ggccgcgccg gagccgcctt cctacgagga
    16801 ggcgctggag gcatcaaggc actacgcggg cttccacacc cacatctatc cgaattgctt
    16861 cgtgtgcggc atgcatcgcg agcgcggcga cggcctgcgc atctttcccg gcgatgcgcg
    16921 cgatggcctg gtggccgcgc cctgggtgcc ggatgcctcg ctcgccggcg acgacggcaa
    16981 ggtcgccccc gagttcatgt ccgcggcgct cgattgtccc ggctacttcg ccgtgcacct
    17041 cgagctgccg ttgctgctcg gcgagttcac cgtgcacatc gaccggcgcg ttcacatcga
    17101 tgagccctgc gtggtggtcg gctggcggct cggctcgagc ggccgcaagc acgtggtcgg
    17161 taccgcgctc tacgacgagg acggccagct gtgcgcgcgg gcgcgcggcc tgtggatcga
    17221 accgcgggtc taggcgggtg gcagggatgc atccggacgt ctcgaaatac aatgacgcgc
    17281 aggatcctgc ggaccgggaa atctgcgagt tgctcgcccg ggaaatggac cgcaatctcc
    17341 ctgaagccga aagcagggta tggcacggtc atccggtgtg gttcctggac ggcaacccga
    17401 tcgccggcta cagccggcag aagccgggaa tccggctcat gttctggagc ggtgcggatt
    17461 tcgacgaacc gggattgaac gtgatcggcc ggaagttcaa ggacgcatcg gtcttcttca
    17521 atgcggcttc cgggatcaaa aagcgcgacc tgtcccgctg gctcgcgaaa gcaaggacga
    17581 tccagtggga ctacaagaac gtcgtcaagc gcaagggtcg gctggagaga ctgaaatcac
    17641 cccggtaggc cacggtggct gctgagccag gggacctcga tgccggtgct ttcggcccga
    17701 ccgtgcattt ctacagaaac tggggagaaa tctgccgagg gtcgctgcgc tgcggaaccg
    17761 ctattctgtg cagcacgcta tcattggccg tcgcgaaacc gagatcgctc acgcgcactt
    17821 gagtggcggt ctatttgagc gcaaaacggc gttttctctc tcccgatcag gccccgtccc
    17881 atgactttag cccctcgtcc tgttcaacag tctctccccg cgctctgccc cctaactgac
    17941 gccatcgagg ccctggctag cgctggcagt attgaagtac gcggggcgat aaacacccgt
    18001 cgcgaagtgg ttgacttcat tctcgacctc gtcggctaca cgcctgatca gctgttgacc
    18061 cagcgccgac tcctcgagcc ctcgttcggg aacggtgact ttcttcttcc cgcaattgaa
    18121 cgcctcctcc gctcgtgggc ggcaaacgat cacttaggtg atcccctcgc ggcgctcgct
    18181 ccgtgcatcc gaggtgtcga gctacataag gagacctttg cgaatactca cgcgaaggtg
    18241 actgcatttc tcattgaccg cggcattcag aggtcgattg ctgcgaagct caccgatacg
    18301 tggctcatcc acggagatta tcttctagct gatcttccgg ccccgttcga cgttgtggtc
    18361 ggtaatccgc cctatgtgcg ccaggagcta atccccgacg tgctcctgca tgagtatcga
    18421 cgccgctaca gtacggtcta cgaccgggcc gacctctaca tcccatttat tgagcggtcc
    18481 ctacgaagcc tcgtcgaccg cggtgtcctc ggcttcatct gtgccgaccg ctggatgaag
    18541 aaccgctatg gggggccgct acgagcactc gtggcgaacg acttccggct caagatctat
    18601 gtggacatga cagacacacc ggcctttcat tccgaggtca ttgcctaccc cgcgatcacc
    18661 gtaatcagcc gtgaaaaatc cggtccgaca cgcgttgcac accggccgcg tatcgcaaaa
    18721 gattgcctca cgaccctatc cgacaaacta accaacgagc agatacggcc tgactgtgtt
    18781 gaggttcgtg aacttgcggg tatcacaact gatggcgagc cgtggatact tgagtcctcc
    18841 gatcagatgg ccctggttcg tcgcctggag cgtgattttc cgacgctcga aggcgccggc
    18901 tgcaaagtcg ggattggtgt tgcaaccggc gcagataagg tctacatcgg ccaatttgac
    18961 gagctcgatg ttgagcctga ccggaagctg cgccttgcaa tgacccgcga catcgtgacg
    19021 ggcagagtcg actggcgcgg gtttggtgtg atcaatccct tcgcagacca gggcggcttg
    19081 gtaaatctcg acaactatcc gcgcttgaaa cgctatctcg atacccataa ggccgaaatt
    19141 cttggccgcc attgtgcgaa gaagattccg gcgaactggt accgaactat cgaccgaata
    19201 acaccgtctc ttgcttgccg gccgaagctg ctcattcccg atatcaaagg tgaggcccag
    19261 attgtctacg aggatggcaa tctctatccg catcacaacc tatatttcat catctctgac
    19321 gagtgggacc tgcgggcctt gcaggccgtt ctcctctccg ggatcgcgcg gatttttgta
    19381 tcgatgtact caacaaagat gcgcggcgga taccttcgct tccaggctca atatttgcgc
    19441 cgaattcggg taccaaactg gaaggacgtt cctgtaggcg tccgtcagga actcaaggag
    19501 gcggccgacg ctcgcgacgt ggtagcctgc gacagagccg tgttcaagct gtatggcttg
    19561 acgcacggag aatccgcagc tctagggggg gtggttaagc cgaatgagtc tggaccttca
    19621 ggactacgac cgcaaagcta gtgacgccgt gcgggccttt tgggccaacc gggagaaggc
    19681 acgacaaaag cagatccagg atggcaagat tgaccaaggc gagcgcgcgg gggtgaccgc
    19741 gggcaagaac atggatggtt ttgtcgattt ggtaagcgac atcgtcaaag caaacggcct
    19801 cgcgcacgcc gacatccatc agcaccgtcc cttgcttgca ctgcccggct actttcgtcc
    19861 tacaaaactg tgggacctgc tcgttatcta caaaggccac ctcattgcgg caatcgagct
    19921 gaagagtcag gttggacctt cgttcggcaa taacttcaac aaccgaacgg aagaggcgat
    19981 cggcaccgct cacgatctat ggaccgccta tcgcgaaggt gcgtttggaa aacaaccaag
    20041 gcctttcgtc gggtggatga tgctggttga ggatgccaag gcctctcgag tgagcatccg
    20101 tgacagctcg ccacatttcc cagtgttttc ggaatttcag ggcgcgtcgt acatcaaacg
    20161 ctacgatctg ttatgtcagc ggcttacgca agaacggctc tactcaacag cggcattaat
    20221 gacaacgccg cggtccgctg tgaatgatgg cgagtttggc gcggaatcag acatgactag
    20281 cttcaagact ttcgtgacga gattggccgg gcacatagcg gctgaaggtg cgcgactcgg
    20341 ctgaatccca gcggttctgc cgaacccggc gcgaaaacca gggcaatgag cggggcacat
    20401 tctgacgacg gcacagcgct atggatctaa cgacggaaca aatcattgaa ctttggtttc
    20461 actacgaaga agtggcgatg cacttcaacg agttgattat ccagtatcga ttgcagctca
    20521 tgggcggggt cggggcgatc ggtgcgctgt cggcttatct cattggagac aaagtcgaag
    20581 atagacagag gcgataccga ctgcgccttg tcgcatcttt gggcctgttt gtccttgtac
    20641 tagccgcggc cttgttggat cttctttact acaacaagct gctcctcgca tcggtcgatg
    20701 tgctgattga tttcgaaaaa gcccaccctc cattgaacat gagtactcgc atcgcagaga
    20761 agatcgcagg acaaggtcta cctgtaattt gcggcgcgta tggtgtcgta cttgggtcgc
    20821 ttggcttgtt caccctgtgg tcatggcggc aacacagcaa gtgcaccgag cggtctctgg
    20881 gagacccaac tgatacctcc tgcgtaggct agtcaggtag ttgcctactg catttctgga
    20941 agcgctcgaa ggttcgctta cccgcgccgc gcgaagaacg caccgaccac tgccgcggcg
    21001 gtgagcagcc agcccggcca ggccgggtcg cgctccagcg cgagccagac gatgccgccg
    21061 agcagcgcgg cggcggcgat cagcattgcg tcgcgccgac gctgcgagcc gcgcagttct
    21121 tcgcgcagtt cgtcgaggcc gtccgccgcc actggcaggc gcagcgtgcc gtcctgggcg
    21181 cgttgcaccg cgccctgcac catgggaggc agcacgcgca gcatcgcgat caggtccgga
    21241 agatgggtcc tgatctcgcc ggcgatgcgc cgcacgctca cccgctcgcg catccactcg
    21301 cgcagcaccg ggctcgcggt tgcgaacacg tcgagatcgg gatagagctc gcgtcccaga
    21361 ccctcgacgt tgagcagtgt cttctgcagc aggatcagct gcggctggat gcgcatgtcg
    21421 aagcggcgcg agatctcgaa caggcgcagc agcacggtgc cgaacgagat ttccttcagc
    21481 ggcttcctga agatcggctc gcacacggtg cgcacggccg attccatttc gtcgatgcgc
    21541 gtgtcgcgcg gcacccagcc ggattcgaca tgcagttcgg cgacgcgccg gtagttgcgg
    21601 tcgaacaccg cgaggaagtt ctcggcgagg tagtgctggt cgcgagggtc gagcgtgccg
    21661 acgatgccga agtcgatcgc cgcatagcgc ggattggccg gatcatcggc gagcacgaag
    21721 atgttgcccg ggtgcatgtc ggcatggaag aagttgtggc ggaatacctg cgtgaagaag
    21781 atctcgacgc cgttgaccgc gagtttgcgc aggtccgtgc ccttcgcgcg cagctgcgcc
    21841 atgtcgctga tctggatgcc gtggatgcgc tccatcacca tcacgtcgac acgacacagg
    21901 tcccagtgca cctccggcac atagagcagc gacgagcctt cgaagttgcg cttcagttgc
    21961 gcggcgttgg ctgcctcgcg catcaggtcg agttcgtcga ggatcgtctt gcggtactcg
    22021 tcgacgattt cccgcgggcg cagccgcggc gccagcgacc agtagcgctg cgcaagctcg
    22081 gcgagcgctt cgagcacctc gaggtcgcgc tcgatgaccg catgcatgcc gggccgcagc
    22141 accttgacga tcacctcgcg cccgtcggcg aggtgcgcga cgtgcacctg ggcgatcgac
    22201 gcggcggcca gcggggtctc gtcgaatgag gcgaacgcct cgccgatcgg ctgaccgtag
    22261 gcgcgctcca ccgccgcgcg tgcctcggcg cccggaaacg gcggcacgcg gtcctgcagc
    22321 ttcgccagct catcggcgat gtcctgcggc agcaggtcgc ggcgcgtgga gaccgcctgg
    22381 ccgaacttga cgaagatcgg gccgagttcc tcgagcgcaa gtcgcaggcg ttcgccacgc
    22441 gagcccccgt ggcggcgcgc gaaccaggtc cagggcgagg ctagcagcag gtagcgcagc
    22501 gggcgataca ggtgggtcgc gcgcacgaag tcgtcgagcc cgtggcgcac gagcacgcgc
    22561 tggatctcga tcaggcgcgc gagcactcgc agcttcaagg ctgatcccgt cgaaccagca
    22621 gctcgatgcg cgccgcgagc cggtcgacat cctcgcgcgt gcggtcgacg tcggcgaaga
    22681 aggcgtcggc ctccgcgcgc ggcaccaggt cggcgtgctc gtgtgcaagg tactcggcga
    22741 cgttgcggct gccggtcgcg gccacctgtc ggccgaagtc cagcacgccg gccgcggcac
    22801 gtgcgagacc gtgcgcggcg gcgtcgccca cgagctgcga gagttcttcc tcgatgtcgg
    22861 gacgcagcag atgtgcaagc tcgcggaagc gttgtgcaag ctgcgcatcg ccgtcgatgc
    22921 gcacgtcgcc gcgacggatc acgccttcgg catcggtacc cagcagcgcg agcagattga
    22981 gcggcgtgcc gccgatttcg gcgtcggccg gcggcgacgc ctcgccctca ggtcgcgtct
    23041 catgggtcag cgtgatgcgc tggccatcgg aggccagcac gaggcgcaat ccggtgccct
    23101 cgatcgtgag cgcagcgcgg cgcccggcga gttcctcgca cagcgcacgc gcctgcggcg
    23161 agcgcggcag gttgcggttg agaagctgct gcacggctga cgtgatcatc ggcgcatcag
    23221 tagcgatagc cgcggtgcac ggccacgatg ccgccgctga gattgtggta gcggcaaccc
    23281 tcgaggcccg cgccgcgcat catgccgagc agtgtctcct ggtcggggtg catgcggatc
    23341 gactcggcga gatagcggta gctcgcctcg tccttagcga ccacgcgtcc gagcagcggc
    23401 aacaggccga acgaataggc gtcgtacagc ggctgcagcg cgtcgatcgg cttcgagaac
    23461 tcgagcacga tcaattgccc gccaggcttc agcgtgcggc gcatcgaggc gagcgcggcg
    23521 gccttgtcgg tgacgttgcg caggccgaag gcgatcgtga cgcagtggaa gctgccgtcc
    23581 gcgaaaggca gctgctcggc attcgcctgc acgcattcga tgccgcgcag gtggccggca
    23641 tctagcaggc agtcgcgacc gtgggcgagc atggccgcat tgatatcggt caagacgacg
    23701 aggccgcccg ggccgacctg acgcgcaagg ccgagcgcga tgtcaccggt accgcccgct
    23761 acgtcgagcg cgtattggcc cggccgcagc cccgtgatcg acagcgcgaa ccgcttccag
    23821 atgcggtgca ccccacccga catcaggtcg ttcatcaggt cgtagttgtc ggccaccgag
    23881 tcgaatacgc cgcgcacgcg ccgcgccttg tcttcgcggc gcacgcgttc atagccgaag
    23941 tcgatggtcg ggtcggcgga aggctggctc aaggtcacct cgcgtgggcc ggcattctag
    24001 cgcgccgccc cgccgcagga tggattgcag cgccggaaag aagatttcac ctttacaagt
    24061 ttgtggaagt ccgctaacaa tcggcgccac ttcaagtccg caaggactgt tgagacgggg
    24121 gtcttaaggg gccgggcacc atgtgcccgg ccccttttct tcagagtcgc tcgccgagct
    24181 cgtacaggta gtcccaggtg tagaggccgc tgtcgtgccc gtcgtcgaac acgagacgca
    24241 cggcgtactg gccgaccggt tgcaccgcac gaatgccgac gccctccttg ccgacgacct
    24301 tgacgcgctg cccggggccg tgtccctgaa cttcggccga cggcgagttg acgcgcaggt
    24361 actcgaacgg cagctcgaag ctgcggccat cgtcgaaact caccttgagc aggcgcgagt
    24421 gctgacgcag gcggatttcc gtacagatgc gggcggccat ggacgcagct tagccctccg
    24481 gcgcgcgcgg tatctatccc gctattgcgg cgagcggcca tcgcctatac tgattcgacg
    24541 cccgccgatc ctggctgccg ctgccgccgt cgactcgaat gccacctact gacacgtccc
    24601 gcccggatta ctttcatcgt gtcgtcgatt gccagtgggc ctgcccggca cacacggacg
    24661 tccccgaata catccggttg atcgcgcagg gtcgcttcac cgacgcctac atggtcaatc
    24721 gcgcgtcgaa cgtgttcccc gggatcctcg ggcgcgtctg cgatcgtccc tgtgagccgg
    24781 cctgccgtcg cggacgcgtc gaggacaagc cggtggcgat ctgtcgcctg aagcgcgtcg
    24841 ccgccgacca caaggacgaa ttccgggatc ggctgccgca gattccggag cagggcaacg
    24901 gcaagcggat cgcgtgcatc ggcgccgggc cggtgtcgct caccgtggcc aacgacctgc
    24961 tgccgctcgg ctaccaggtc accatcttcg agcagttccg cgagcctggc gggctgatgc
    25021 gcaccaacat cccgtccttt cggctgccgc agacggtgct cgacgaggag attggcgcca
    25081 tcgtcgacat gggtgccgag ctcaagctca atcacccgat aaagagcatg cgcgcactgc
    25141 tcgagggcgg cggcttcgac gcggtgttcg tcgggtccgg tgcaccgaag ggcaaggaac
    25201 tgaaactgcc gggccgggaa gagggcgcgg ccaacatcca catcggcatc aactggctcg
    25261 aatcggtggc cttcggtcac ctgaatgcga tcggccggga ggtgctgatc atcggcgtcg
    25321 gcaacacggc gatggactgc tgtcgcacct cgctgcgcct cggcgccgcg cacgtcaagg
    25381 tcatggcgcg caagccacgc cagttcttca aggcctcggc gtgggaactc gacgacgcag
    25441 aggaagagaa cgtcgagatc atcgtcaacc gctcgccgaa gcggttcgtc atcgagcagg
    25501 gcaggctcac gggcatgctg ttcggcgtga tggaatacga catcgatgcc ggcgggcgca
    25561 tcaccgccga gcgcgtcgcc ggcgaggaat tcctgccggc cgacgatgtg atcctcgcca
    25621 tcgggcagga aaacgcattt ccgtggatcg agcgcgacct cggcatcgag ttcgacaagt
    25681 ggagcgtgcc gaaggtcgat gcgacgacct tccagtcgac gcgaccggag gtcttcttcg
    25741 gcggcgacgc ggccttcgga ccgaagaaca tcatctgggc ggtcgagcat ggccaccagg
    25801 ccgcgatttc gatccatcgc cactgccagg gcgtggcggt caccgagcgg ctggcgccgg
    25861 gcgtgaacct gcagagccgc aagatgggca tgcacgagtg gtcgtacaag aacgactaca
    25921 acccggtcga gcggcggctg atgccgcatg tgtcgctcaa ggaacgcttc aagaagctca
    25981 acatcgaggt cgagctcggc ttcactgccg agcaggccgc ggccgaggtg cagcgctgcc
    26041 tcaactgcga catccagacg gtgttcgagg cgaagctgtg catcgagtgc gatgcctgca
    26101 tcgacatctg cccgaccgac tgcctgacca tcaccacgga cggcaccgag gacgatctgc
    26161 gcacgcggct gcgggcgccg gccgcgaacc tcgagcaggc gctgtacgtc tcggctccgc
    26221 tgaagcagac cggccgggtg atggtgaagg atgaagacct ctgcgtgcac tgcggcttgt
    26281 gcgcggaacg ttgcccgaca gccgcctggg acatgcagaa gtcgtacgtg gcgtggccgc
    26341 acgccaagga tcagccggaa aaatgccagc cctcactgcc caagatcgcg taaacgactt
    26401 cgtcatcaag atcgcaacgg tgaacggcac gggctccgcc agtgccaacg ccctgctgat
    26461 gaaggcgatt ttccgcagcg gcgtgccggt gatgggcaag aattacttcc cgtcgaacat
    26521 ccagggactg ccgacctggt acgagatccg cgtaacccgg gatgcgcaca ccgcgcgctc
    26581 cggccgcatc gacctgatgg tcgccatgaa tgccgagacc tacggcaagg acatgcgcga
    26641 ggtcgacagc ggcggttacc tgatctacga ctcgacctgg ccgcgcccgg cgctgctctc
    26701 gcgcgaggac atcaccgtgc tcggcgtgcc gctcgcgcgt ctgtgcaacg aacgcttcaa
    26761 gggcgtgcgc acgcgcatcc tgatgaagaa catctgctac gcgggagtgc tcgcggcact
    26821 gctcgacatc gacctcgagc ggatgcggga gctgctcgcc gagacctacg ccaagaagcc
    26881 gcagctcgcc gacagcaaca tggaggcggt gcagcttggc tacgactacg cgaagcagca
    26941 tttcaactgc ccgctgcccc tgcgcgtcgc gccgctcgac cgcacggccg gccacatcct
    27001 gatcgacggc aataccgcga ccgggctcgg cgcgatgttc gccggcgcca cggtgggcgc
    27061 ctggtatccg atcacgcctt ccacttcggt gatggacgcg tacaaggcct tctgcgacaa
    27121 gtggcgcgtc gatccggaga ccggccgcag gaacttcgcg ttcatccagg ccgaggacga
    27181 actcgcggcg atcggcatgg tgctcggcgc ctcgtggaac ggcgcacgtg cgttcaccgc
    27241 gacctcgggc cccggcatct cgctgatgaa cgaattcctc gggctcggct actacgccga
    27301 gatccccgcc gtggtgttcg acgtgcagcg ggtcggcccc tcgaccggca tgccgacccg
    27361 cacccagcag tgcgacctgc tgtcctgcgc ctatgcctcg cacggggaca ccaagcacgt
    27421 atgcctgtac cccgcgagtc ccgaggaatg cttctacttc gccatcgcgg cgttcgacct
    27481 cgccgagcgg ctgcagacgc cggtcatggt gctgtcggac ctcgatatcg gcatgaacga
    27541 ctggatgtgc cccgagttca agtgggatga ctcctaccgg cccgaccgcg gcaaggtgct
    27601 gccgctcgag gaaatcgtca agctcgaaaa gttctaccgc tacctcgaca aggacgacga
    27661 cggcatcccg taccgcaccc tcccgggcgt gcatcccaag gcggcgtatt tcacgcgcgg
    27721 ctccgggcac acgcagtacg gcgcctatac cgaggattcg gccgaatacc agatcgtcgt
    27781 cgaccggctg ctgcgcaagt tcggtaccgc gaagactctc gtgccgaagc cggtcatcga
    27841 aatggccgac aagcgcaccg atgtcgcgat cgtcgcggcg ggcagcagcg atggcgccgt
    27901 gcgcgaggcg ctcgtcgtgc tggccggcat gggcatcccg gtgaactacc tgcgcgtgcg
    27961 cgcgttcccg ttcaacgacg aagtcgagcg cttccttgcc gagcaccgtc ttgtgttcgt
    28021 cgtcgagcag aaccgcgacg cgcagttgcg ctcgctgctg acgctcgaga cccaggtcga
    28081 gaagtccagg ctgcgctcgc tgctgcacta cagcggcctg ccgatctcct cgagcttcat
    28141 catcgaaggt gtgctgaccg aggtcgcacc gcggccgctg cgctcggtcg ctccggtgca
    28201 cgccaccggc acgccggcgg agtgagccca tgaccttcat accgaagccg aaagttcgcc
    28261 acccgtcgct gccgcgcaat gatctcggcc tcacgcgccg cgactacgag ggcgtgctgt
    28321 ccacgctgtg cgcgggctgc ggccacgatt cgatcaccgc cgcgctcacc gaggcgatct
    28381 gggggctgtc gctcgagccg cagaacatcg ccaagctctc gggcatcggc tgttcgtcga
    28441 agaccacggc ctatttcgtc ggcggcgcgc acggcttcaa ttcggtgcac gggcgcatgc
    28501 cggcgatcgc gatgggtgcg aacgcggcga accgcaagct gagctacatc ggcgtctccg
    28561 gcgacggcga cacgctgtcg atcggcctcg ggcagttctg ccacgcgatc cgccgcaacc
    28621 tgaacatgct ctacgtgatc gagaacaacg gcgtctacgg cctcaccaag gggcagttct
    28681 cggcctcggc ggatatcggc acgaaggcga agaagggcga gaagaacctg caaccgccga
    28741 tcgatccggt gcagctcgcc atgagcctcg gcgcctcgtt cgtcgcgcgc agtttctcgg
    28801 gcgacaagcg ccagctcgtg ccgctgatcc aggccggcct gcgccatcgc ggcttcgcgc
    28861 tgatcgacgt gctctcgccc tgtgtcacct tcaacgacca cgagggctcg accaagagct
    28921 acgcctacac gcgcgagcac tacgagccgg tggtcaacgc ggatttcgtg ccgctcgagc
    28981 gcgagatcgt cgccgattac gccgagggtg aatccctgcc gatcgtgatg cacgacggca
    29041 gccgtatcct gctgcgcaag tttgcgcagg attacgaccc cacggatcgt gtcgccgcga
    29101 gtgccttcat ccaggcgcgg cagaagcagg gcgagttcct gaccggcctg ctctacatcg
    29161 acgagtcgca gccggagttc cacgaggtga atggcacgcc ggacgcgccg ctcaacagcc
    29221 tgccgttcgc ggcgctgtcg ccgggtgcgc aggggctcgc gaagatcctg tcgcgatatc
    29281 gctgaacgtg cggcgacggg ctgctagact cgccccgctt tcacgcacaa ccggagtcga
    29341 caatgggtaa aggtgacccc cgcagccgtc gcggcaagat ctaccagggt tcgttcggca
    29401 agacacggcc caagaagaag gcggcgaaga agaaggccgc aggcaaatcc actaggaagt
    29461 agccgggtcc ggcgcgcggg tgactttgcg cgccgccact tcgtggatca gttcgacggc
    29521 cgtgcgatcg cacggcggca gctcgatgtc ggtggtgtcg agcggcgtga tccgatttcc
    29581 ccagcgcacg aggtgcgcgc agaaggtgag gccgccgccg aaggccggca tcagcaccca
    29641 gccgcccttc tcgatgcggc ccgactcgag cgcctcgcac agtccgaccg gcacggtcgc
    29701 cgaggacatg tttccgtagc gctgcacgtt ggtgaacacg cgctccatag ggatgccggc
    29761 gcgcttcgcc acggcctcga tgatccgcag gttcgcctgg tggggcacgg cgagctggat
    29821 gtcgtcgggg gtgagcccgc gcttgccgag cgcggctgcg ctcgcctcgc tcatgccgag
    29881 caccgcgcgc ttgaagatct cctgcccttc gaactgccag gaggtgacgc cgtacaggcg
    29941 cccctggttc gcgtagttgc cacccatgcc gtggatgcgc agggtctcgc gcgcatcgcc
    30001 gtaacagccg agtttttccg cgagcaggcc ttcttccgag tccgtcgcct gcaggacgac
    30061 cgcagcggca ccgtcgccga acagcaccgc gacgttgcgg tccgaccagt ccatgtacgg
    30121 cgagatgatc tcggcgccga cgaccagcgc gttgctcacg acgccgttcc tgatcatcgc
    30181 cgtggcgctc gacagcccgt agaggaaact ggtgcaggcc gtgttgacgt ccatggcggc
    30241 gcagccctgc gcgccgatgc gctgctgcag gcctgaagcc atgttcggca cctggtcctc
    30301 gaagctgcag gttccgaaaa cgatcagttc gatttcgcgt cccgccatgc cggcgcaggc
    30361 cagcgcccgc gcggcggcga cgtgggcgag ctcggccatc gggacatgcg agatgcggcg
    30421 ttcacggatg ccggtgcggc tcgtgatcca ctcgtcgctc gtctccagca tgcccgcaag
    30481 gtcctgattg gaaaggacgg ccggcggcag gcacttgccc cagccggtga ttgctgcgta
    30541 ggtggtcatg gccgcgcagc atacgtgccg gggccccggc gcgcacctgt cagtcgtttc
    30601 ctacgcgcgc cgcgccacag gctgactgcc aggcggcatg gcgccgcgta gcttcggact
    30661 tgcgaacgcc ggtgccttcg ccccccgtcg agtaccggcc tcctggacgc cttcacgcga
    30721 gtgaaggcgt cttttttcgt gccgtcgcgg cgtaggacag ggcacgcgca atccggccgc
    30781 gcggacgaca tcctcggcgg gctgtcccgc atacgctcgc ctgccgtgtc acgacaggac
    30841 cccacgatga agtaccagag caccggcgat gcctggctcg cgattgccgt catcttcctg
    30901 gtcatcgcga tcgtggccgc cgtgttcggc ttcggcggca tcgcggcggg cgcggccggg
    30961 atcgcgagga tcctgttctt cgtcttcctg atcgccttcg ccgtgacgct gctggtcggg
    31021 ctcttcaggc gcgcctagtc ccctcgcaca gccgctgctg ctagcatggc gcgtgtggtt
    31081 tgctggcggg gtggcaggtg actgagggga acgggctgcc gtggcgccaa tatcgcagcg
    31141 tccatctcga tttctgggat ggcctgcgat tgctgcccgg ctcgatctgg ctgccgatcg
    31201 tcgcgatctc gctcctcgcc ctcggcgtgt tcctgatgaa cgaatggtcg atgggccgca
    31261 tccgcatcgc cgccgagggc gtcaaccgca ccctcggcat gcagaacgag gtgatcgcgc
    31321 tgcgctcgca gctggtcgac ctcgagagcg cgcagcgggg atatctgctc accaccaaca
    31381 ggcgttacct ggctccctac gaggccgcgc tgccggagct gcacgtgatc agctccaagc
    31441 tgcgcacgct cgccgcggcg gatgccgagg tgctccagca cgtcacgcgg ctcgaggagc
    31501 tgcgtggcat caagatcgcg gaggtgaccg cgtcgatctc gctgacgcag cgcggtgacc
    31561 gcgccagcgc catggcgatc ctgcgcaccg gcgagggccg caaggtcatg gacgagttcc
    31621 gcgccgccgc ccatgagctc ctgcaactgc tcgacacccg gctcgaggcg ctgcgcgccg
    31681 agcaggcgcg cagcctcgtg ctgtctcgct acgccttcgc ggcgctggcg ctgctcgcgc
    31741 tcgcgctgat cctgctggtg gtgcgcctgt tcgtcgccaa ttcgctgcgc cgcgaggaac
    31801 agcgccacga ggaggagcac gagcgccgca ggctcgaggt cgtcatcgag gaccgcacgc
    31861 gcgagctgtc gaggctcacg acccacctgc agagcgcttc ggaacaggag aaggccgatc
    31921 tcgcgcgcga cctgcacgac gaactgggcg ggctgctgac cgcagcgaag atggatctcg
    31981 cctggctgca gggccgttcc atcacgatgg accccgaagt gcgcgagaag ctcgacgagc
    32041 tggtgcgcac ggtgaccgaa gcgatgcacc tgaagcgccg cgtcgtcgaa aacctgcgac
    32101 cggcgctgct cgatcatttc ggcctgccga ccgcgctcga ggcctatttc gacgaaacct
    32161 gcaagcgtgc ggggctctcg tgccgcacgt cgattccgga agacacggca ccgattccgc
    32221 agcagctcgc gatcgcgctg ttccgcgtcg gccaggaatc gctcaccaac atcatccgcc
    32281 acgccagggc gaggaacgtc gagatgcagt tcgaggtgac cgagtcgggc taccacctca
    32341 cggtgagcga cgacggcgtc ggcatcgatc cggagcgcgc gcgcagttcg cacggcctct
    32401 ccggcatgcg ccatcgcatc gtgagcctga acgggtcgtt cgacatccag agtgtcccgg
    32461 gggaggggac gcggctcgat atcctggtgc cgttcgcgga agccggagta cagggctatc
    32521 agctgtcagc gtgacgatag tgggcttcga ggagtggccg gacctgcagg aactgctgca
    32581 tcttgtcgag gaagtggtcg gcgccgacct cgatgcagcg ctggcgaacg gtgggcagcg
    32641 cgtagttggt gagcacgatg atcagcggct gccggcgcgc ggcgtatgag cgccgggcgt
    32701 ggcgtatcgt cgtgatgccg ctgccctccc tcagttcgac gtcgacgacc agtgcgtcgt
    32761 agtcattgcc atcgatcagt gcggtcgcct cgcgttcggt ttccgcggtg ccggtcacct
    32821 gcatcacgcc cggcacggtg agcatgccta tcatgcgctc gcgcagcggg cgcgaatcct
    32881 cgatcagcag gatgcgcagt tccccgccgg gcagctcccg ccggccgccc gtctgcccgg
    32941 cgttgcgtac ggctgcacgg cgatcggcgg tcccggtggc gtggagatcg gcaggcatgg
    33001 ctactgcagg aggctgttct tgatcgcgta gtaggtgatg tcggcgttgg tcttcatgcc
    33061 catcttgtcg aggatgcgcg agcgataggt gctgaccgtc ttgacgctga ggaacagctt
    33121 gttcgcgatc tcggtcacgg tcttgccctc ggcgagcttc acgaagatct ggaattcgcg
    33181 ctccgacagc acgcggtggc gcggctggtc accggtgccg tcgatgccgg acagcagcag
    33241 gtcggcgagc tcggggccga cgtagcggtt gccgcgcgcg accagccgca cggcacgcag
    33301 cagttccgac tcctcgacgc ccttgctgat gaagccgttg gcgccgccac gcagcacgtt
    33361 gatgccgaac tgcttctccg gatagctgct cagcaccagc acgccgatgc cctcgtaatg
    33421 cgagcgcacg tgcttcagca cgtcgagccc cgacatatcg ggcagcgaca ggtcgagcag
    33481 cagcacgttg caggcgtgtt ccttgagcgc ggcgatgaca tccttgcccg agcggcattc
    33541 ggccgcgaca cgcaggtcgg gctgcgagga aatgatctgt ctcaggcctg cccgcacgac
    33601 ctgatgatcg tccccgatga tgacattgac ctgaggcatt cccctcgcct agttgtcgta
    33661 aagcagcccg atcacggcgc ggcgcgcgcg cttccaggtc gaggtggtca ccgcggccgc
    33721 gcgctggatc tcgccgcgtg ccgccgcgat gtgccagcgc atgatccccg gtaccgcgct
    33781 cgtcgccgca tcgaccggat actttgcgcg aacgcgcgcg agactgctgt aggaattctc
    33841 cgacatgacc tcaacggacc tctgcccata cccgccggca gccaaaatag cgccgtgtgc
    33901 gcgcctggca cagggcggga gggacgtgtc gcgcgtagga cgaggctgac atggcacaat
    33961 gggcgccagg cgcagcggag gcacgcaagt atgtcctcga acgtcgagag ggcgccgggc
    34021 gagaagctca cggtgcagtt cgaagcccgc aagtgcatcc gttcgcggca ttgcgtgctg
    34081 ggcgacccgc gcgttttcgt cccgaacgca gacggcgagc cgaacgtgga aggcgagtgg
    34141 atccaccccg acgcggcgtc cgtcgacgcg gtggtcgaaa tcgcgcacaa gtgcccatcg
    34201 ggcgcgatta cctacgagcg gatcgatggc gggccgcagg aggcgccgcc gcccgtcaat
    34261 gtcgtgcggg tgcgcgagaa cgggccgctc gccttccacg ccgaaatgca cttctacggt
    34321 ggcggcagcg cactgcgcgc ggcgctgtgc cgctgcggtg cttcgaagtc gaaaccgttc
    34381 tgcgacacga gccatgccga cgcgggtttt gccgccagcg gcgaggcgcc gaccagggat
    34441 tcccccgcgc tcgcggtgtg caacggtccg ctcgaggtca tcccggtcga gaacgggccg
    34501 ctgcacgtga aaggcaatct cgaagtggtg accggcacgg gccgcaccgt ggagcgcgtt
    34561 accgagtgct ggctgtgccg ctgcggcggg tcgggcggca agccgttctg tgacggtacc
    34621 catcgcagga tcgggttcac ggccgcgggg ctggcgccca ggcgcaagta gcctcgcgag
    34681 tgccgatcag gcgtgcccgc cgccggcgag gcgtgcagcc agccagccgg agatgtcgtc
    34741 gatttcggcg cgacacacct cgtgctgcat tggataggtc ttccattcca ccgggtatcc
    34801 gaggcgctgc agtgcatcgc gcgacgcctc gccgagcccg atcggcagca ccgggtcgta
    34861 ctgcccgtgg cacatgagga tggggagatc gcgatttgcg gccgacgcct cgcgctcgag
    34921 actggcgtgc agcggcaggt aggtggacag ggccatgacg ccgccgagcc gcgccgggta
    34981 acgcagcgcc gcgtgcagcg ccacggcgcc gccctgcgag aagccggcga tcacgatgcg
    35041 cgcgggcgcg actccccggt cgatctcacg ctggacgagc tggtcgacgc gcgccgcgga
    35101 ggcgcgcgtg ccggcctcgt cctggcgccc ctcggtcgac agctcgcgga tgtcgtacca
    35161 cgcgcgcatc ggcaggccgc cgttcagcgt gactggccgg accggcgcat gcgggaagca
    35221 gaagcgcacc gccacggtgg gcggcagccg cagctccggc acgatgggca cgaagtcgtg
    35281 cccgtcggcg cccagtccgt gcagccagat aacgcttgca tcgaccgccg gaacgatgcc
    35341 ggcgctcgcc gctgggacat cgacgacgac ttcgtcgccg ctcacaggat gtaccgcgac
    35401 aggtcctcgt ccttggcgag attgccgagt cgcgcatcga cataggccgc atccacggtc
    35461 acggtgctgc cgtggcgctc ggtggcttcg aaggaaatct cctcgaggag ccgctccatc
    35521 acggtgtgca ggcggcgtgc gccgatgttc tcggtacgct cgttgacctg cgccgcgatc
    35581 tccgcgattc gccgcacgcc gtccgcggcg aatacgaggc gcacgccctc ggtttccatc
    35641 agcgcggcgt actgcgtggt gagcgccgca tcgggctcgg tcaggatgcg cacgaaatcg
    35701 tcgacgccga gcgatgcgag ctctacgcgg atcggcaggc ggccctgcag ctcgggaatc
    35761 agatccgaag gcttcgacag gtggaacgcg cccgaggcga tgaacagcac gtgatcggtc
    35821 ttcacggcgc cgtacctggt agcgacggtc gagccctcca cgagcggcag caggtcgcgc
    35881 tgcacgccct cgcgcgacac gtcggcgccg aacgattcct gccgccgggt caccttgtcg
    35941 atctcgtcga tgaaaacgat gccgttctgc tcggcgttgg ccactgcgcg cgccttcagc
    36001 tcctcctcgt tgacgagctt ggccgcttcc tcgtcgacca gcagcttgag cgcctccttg
    36061 atcttcacct tgcgcgaatg cgtgcggccg ccggcgaggt tctggaacat cgaggaaagc
    36121 tgctgctgca tttcctccat gccgggaggc gccatgatgt cgacgcccgc ggggatcgcg
    36181 cgcaactcga tctcgatctc gcgatcgtcg agctggcgct cgcgcagcat cttgcggaac
    36241 ttctggcgcg tctcggcatc gcgcggcacc ggcggctcgt cggtggagaa gcccatggcg
    36301 cgcggcttgg gcagcagcgc atcgagcacg cgctcctcgg cggcgtcgag agcccgctcg
    36361 cgcaccttct cgacttcctg ctcgcgggcc atcttgaccg cgacatcgac caggtccttg
    36421 acgatcgagt cgacgtcgcg gccgacatag ccgacttcgg tgaatttggt cgcctcgatc
    36481 tttacgaacg gcgcattggc gagcttcgcg agccggcgcg cgatctcggt cttgccgcaa
    36541 cccgtggggc cgatcatcag gatgttcttc ggcgtgatct cggggcgcag gtcgtcgtcg
    36601 acctgcatgc gtcgccagcg gttgcgcagc gcgatggcga cggcacgttt ggcgtcgccc
    36661 tggccgacga tgtgcttgtc gagctcgcgc acgatctgct gcggcgtgag cgcggctccg
    36721 ccgggaagct tcacttcggc gctcattact tcagttcctc gatcacgaaa ttgcggttgg
    36781 tgtagatgca gatgtcggcg gcgatgccga gcgcgcgctc gacgatcgtg cgcgcatcga
    36841 gttcggtgtt ctcgacgagc gcgcgcgccg cggcctgcgc gaaagcgccg cccgagccga
    36901 tcgcgacgag gtcgtgttcg ggctcgatca cgtcgccgtt gccggagatc acgagcagct
    36961 tttcccggtc gcccacgagc agcagcgcct cgagccgacg caggtagcgg tccgaacgcc
    37021 agtctttggc aatctcgatc gccgcgcggg tcaggttgtt gccgtgcttc tcgagcttgc
    37081 cctcgaagcg ctcgaacagc gtgaaggcat cggcagtgcc gcccgcgaag cccgcgagca
    37141 ccttgccgcc gccgagcgga cgcaccttgc gggcattgcc cttcatcacg gtgttgccga
    37201 gcgtgacctg gccatcgccg ccgacggcga cggcaccgtt gcgtcgcacc ccgaggatgg
    37261 tggtgccgtg gggattccac acttcgctca tggggaaacc agcctacttc ttgcgcgcgc
    37321 gcggatgcgc ggaatcatag atgcgggcga gatgctggaa atcaaggtgc gtgtagacct
    37381 gggtggtcga gatgtcggcg tggccgagca attcctgcac gccgcgaaga tccctactgg
    37441 attcaagtag atgcgtcgca aacgaatgcc tgaaaaggtg cgggtgcacg tgcactccga
    37501 gcccctgccg gcgcgcccag taggcgaccc gcagctgcac ggcccgcggg cccatgcgtg
    37561 tgccattgcg cccgacgaac acggcagtct cgccggcccg ggcgagcgcc gcgcgttcgc
    37621 gcagccagct gcgcagtgcc gcgagcgccg ggcggcccac cggcaggatg cgggtcttgg
    37681 cacccttgcc caatacacgc acggtgcggt cggcgaggtc gaggtcgccg atgtcgagcg
    37741 ccacgatctc ggcgaggcgc aggccggacg agtaggcgag ctccatcagc gcgcggtcgc
    37801 gcagcgcgag cgggtcggtc gctttgacgt tcagcagctg cgtcaccttg tcggcatcga
    37861 gcgtgcccgg caggcgcttg ccggccttcg gcgcgcggac atcgtgcgcg gggttcgtgc
    37921 tgatggcgcc ctcgcgcacg aggaaggtgc agaacgagcg caccgccgac aggcgccgct
    37981 gcacgctgcg cggcgcgagg cccgcggcgt gcgatcgcgc ggcgaacagc ctcacatgct
    38041 ggctgtcgag gcgggcgggg tcggcgatac gctcggtctc gcagaagcgc acgagggccg
    38101 cgaggtcgcg cgcgtagttc gagtccgtgt gcggcgagag ccgccgctcg gtggcaagat
    38161 gccgccggaa gcgcgcgatc cagtcagcga tagacgacgg catccgcgat cagctcgccc
    38221 atgcgggcga ggaactcggt actcatgccg ggatggaagc gctcccggtc gtggctgccg
    38281 agtgccagca ggccaaccga gccgtgttcg ccgagcggca cgagcgcgac cgagccgatc
    38341 tcggtggagt cggtgccgaa caggaactgg cgctgtgtgt cgcgcacctg cccgcagcgg
    38401 ggtttgccgc tcgcgaacag gccctcgaac gacttgaagt tcgggtcgtc ggccgacacg
    38461 gcgcgcagga agcgctcggg cagtccctcg aagccgcgtt cgttgccgtt cacgagaatc
    38521 agcgtggcgt ggaaggcgtc gaagtcctcg cgcaggctcg cttcgatctg ggcagccatg
    38581 tcctggcgcg aagtggcgcg cagcagccgg cgcgtgaagc gctggatctt cccggtcagc
    38641 acatcgttcg cccgcgcgac gcgcacgaaa tccgcaagct tgccctcgag ctcggcctga
    38701 cgctcgcgca tcacctcgac ctggcgctcg accagcgaaa tggtcgacgc cgagcgcgcg
    38761 tgcggcagcc gcatgcgcgc gaggatcgcc tggtggcgct cgaagaaatc ggggttgagc
    38821 tgcaggtagc gcgcgatcga ctcttcgtcg atcgtctcgg tcggcagtcc gcgggcctct
    38881 cttgtcgtca tgggttctcc gccccccggg acgcatgcgc gccgttcacg tcgacgctgc
    38941 cggtgaaaga tacttcggcc ggacccgtga gccaaatatt cttgccggga ccgggccagg
    39001 tgatcgtgag ttccccgccc gcgacgcgca cgctgacgcg ttcatccagg cggccgtggc
    39061 gacggccgac ggcgacggcc gcgcaggcgc cggtaccgca ggcaagcgtt tcgccggtgc
    39121 cccgctcgtg gacccgcaat atgatgtggt tgcgatccac gatctccatg aagccgacgt
    39181 tgacgcgctt cgggaagcgc ggatggtgct cgatcgccgg ccccagccga tcgaccggcg
    39241 ccgcggccgc cgatgcgacg gtcagcacgg cgtgcggatt gcccatcgac accgcgccga
    39301 tctcgacttc gctgccgccg acgtcgagca ggtacacatg cgcctcggtg gaggcgtcga
    39361 acggcagcgc cgccggattg aagtccggca cgcccatgtc gaccgagacg aggccgcccg
    39421 cttcgatgcg ggcccggatg aggccgccgg ggctgtccat cgcgagttca ccatcgcgcg
    39481 ccttgccgcg ctgcgcgagc agcgcggcga tgcagcgcgc accgttgccg cattgctcga
    39541 cctcctggcc gtcggcattg aagatgcggt aataggtcgc ggtgccctca cggcgcgggg
    39601 gctcgagcat cagcgcctga tcgaaaccga tgccggtgtg gcggtcggcc agacggcgga
    39661 gctcggccgc ggacggcggc gtggcgccgg cgggcgcgtc gaacacgatg aagtcgttgc
    39721 cgagaccgtg catcttcgtg aagtcgacgc gcattggcga agtcgcgcgc ttcaaagggc
    39781 cgcccggtcg cggaacaggc agcgatccat caccgtgaaa attccggctg cctgcgcccg
    39841 gctggccgcg gcctcatcga tcacgccgtc ctgcaaccag agcgccggca tgccgaggcg
    39901 gatgcagtcg tcgacgaggc cggcgacgtg ctcggggcgg cggaacacgt cgacgatatc
    39961 gggcctggtg cccgccggca gcgcatcggc gaggtgatcg agatcgggca ccgcacgttc
    40021 gcccaggatg gtggtggcgg cgggattgac cggaatgatc cggtaaccgt agcgctgcaa
    40081 ttgccgtgcg acgtcgtggc tgggccgcga cgggtcggtc gagaggccga cgacggcgat
    40141 ggtcctggcc gtgcgcagca gctgctcgat cgcagcctgg tcgggattgc ggaacatccg
    40201 cgtagtttac ggcggatcga gcagcggcgt gaccccgtga gcagcggccg atcgcgcgct
    40261 cggcactgcg cccataatgg gcggatgaga gtgaggttgc tgccgtcggc gcgggagttc
    40321 gatgcgcggc cggacgagcc ggtgctggcg gcggcgctgc gcgggcattt taacctgccg
    40381 cacagctgca ggagcggcca ctgctcgtcc tgtcgcgctc gcatcgtcgc ggggcaggtg
    40441 tggtatcccg gcgcgcggcc ccccggcctg acggcggagg aagaggccgc cgggttggcg
    40501 ctgctctgcc aggcgcgcgc ggcgagcgat ctcgtgatcg aagcgcgcga gatccgccgc
    40561 gtcaccgatg tcgagatcaa gactctgccg tgtcgcatcg agacgcgcga gcggctcgcg
    40621 ccggatgtgc tgcgattgca gctgcgcctg ccggcggtcg agcagttcca cttcctgccg
    40681 ggacagtacc tcgacgtgct gctagacggt ggccggcggc gcagcttttc gatcgcctcg
    40741 ccaccgcacg atgcggcgca actggaactg catgtgcggc gcgtgccggg cggggacttc
    40801 accggggagc tctttgcaca cgacgcgccg ggcgcgctgt tgcgcatcga aggtccgatc
    40861 ggccagttcg tctacgtgga tgcgccgggg cccgtggtct tcgttgccgg cggcaccggg
    40921 tttgcaccgg tgaagtcgat gctgcgccat gccctcgaac gcggtacgcc gcgggacatc
    40981 cacttctact ggggcgcgcg cacgccggtc gatctctacg aggaagcgct gctgtacgca
    41041 tgggcggcgc gccatccgcg ctttcactac accggtgtcc tgtcggaagc gccgccggat
    41101 tcagccggcc acgagtcggg ctgggtgcac gaggcggtcc tcgcgcatca tccggatctg
    41161 tcgactttcg aggtgtatgc cgcaggcccg ccggccatgg tcgaggcgat acgcgcgcgt
    41221 ttcccggcgc agggtcttgc gccgcggcgg ctgcacttcg attccttcga ctacgcgccg
    41281 cgctgacgcg cgcgcggctc aggcgtagcg acgcgtggtg gcgaccaggc agggcgtgga
    41341 gcggcaggcc gcgtgcaact ggcgacgtcg cagtgccacg ctcgtggtgt acgacatggc
    41401 cacggcatcg gcgagcagct ggcgcatgcc gatcgcctgg cgatgcagat actcggcgag
    41461 cgcgagcgac atgcggatgt gctgttgcac ccagcagtcg tcggtgccgt ccggcagcgc
    41521 ggcgagcatc gcctcggcga gaacgagatc gagaccgagc ttctgcgcgt cggcctgcag
    41581 ccattcggtg accagtgtgc accgcgcggc ggtgagcatg aaggcgtatt cgcgctcgac
    41641 gatcgccgtc atgtgagcgg cgagttcgcc gctcacgccc ggctcgtccg gataatcacg
    41701 ggtcggaaac agcacttcga gcggcgccca ggcctggatc gtgtcggcga cccagcggcg
    41761 cgcggcggcg gacggcgcag gagtaccgag caccgcggcg acccgcccca gcgcccggtt
    41821 gcgcagcgtg ctggtcgcag gctcgaaggc gcgggcccag ccgctgcggg catggtcggc
    41881 cagccagtcg tcgaaggcct gctcggcttg tccataagcg ttggcggctg tgaccttcat
    41941 ccctgaaact cccgtgatga gagccacata tgccgcatcg gccgcaggtc ctgcgttgat
    42001 gcgcgtcaca ggcggaagcg ttcgcgcagg tctccggcca tccggcggct cacggcgagc
    42061 cggtccgcgt ggccgcgcag gcggacgaaa tactgcccgt cagggtcgcg ctcgatgcgc
    42121 tcgaggtgcg cgacgctgac gagtgcgctg cggtggatgc gcacgtagcc gtcggcaaac
    42181 tcctcctcga gcaggcgcag cgactcctcg atcaggtcct cgccgccttc atggtgcacg
    42241 gtcgtgtact tctggtcggc gacgaaacag cggatttctc ccagcggaat caacttgata
    42301 ccgtcgcgac ggcgtaccgg tacatggctg cgccgggggg ccggcgcggc cgtcgcggat
    42361 tctgcgcgcg ccatctgcgg ccgggtcagg cggcggcagg ccgcgatcgc agccaggagc
    42421 ttgtccttgc ggatgggttt cagaaggtag ccggcggcct gtgcctcgaa cgcgttgacc
    42481 gcgtattcgt cataagccgt cgtgaatatg acagcgggcg gctcgctcat ccgcgcaatg
    42541 cgctgcgcgg cttcgaggcc gtccatgtcg ggcatgcgca cgtcgatcag caccacgtcc
    42601 ggcgacaggt ccatcgtcag ctcgagcgct tcgcgtccgc tcgcgccctc gccgacgacc
    42661 tgcacgtcgt cgatctccgc gagcaggctc gcgagccgcg cgcgcgccgg cggctcgtcg
    42721 tcaacgatga gcgtcttgag cttcatgcgg gaacctgagc gtgacgatgt actcgtcgtc
    42781 gaagcgcccg gccttcaccg ccgcgcgcgt gccgtacatc agcgacaggc gctcgcggat
    42841 gttggcgagc gcgaggcggt tgccgtcgcg cggcacgagg ccctcgggca ccgggttgcg
    42901 caccacgatg gtgacgagcc cgttggcgtg ctcgccggtc accttgaccg tgccgccgcc
    42961 gctgcgcggc tcgatgccgt ggtagatcgc gttttctagc agcggctgaa tcatcaggct
    43021 cggcacgatg gcatccggcg gcaccgcatc gacgcgccat tcgacgtgca ggcgttcgcc
    43081 gaggcgcagt ctctcgatgc gctcataggt cctcgcgacc tcgatctcct cggcgagcgt
    43141 gatctggtca cgcttctcgg caaggctcac gcggaacaga tcggcgaggt cctgcaccgc
    43201 ttcctcggcc agcgccgggt gcgaacgcgt gagcgcggcg atcgtgttca tgctgttgaa
    43261 caggaagtgc ggccggatgc gcgcctgcag cgcatgcacg cgcgcgcgcg actgcatttc
    43321 gatgttgcgg cgccattcat gggtcacgta gaggtaacgc agcgcaagcg cggtcacgat
    43381 gaaggcgatg acgacattgc gcaggatgaa cggcaggtgc tcgcgtggga acagccgccc
    43441 gacctcgatc ggcgccagca tgtcgctcgc gcccagccgg tacgccacct cggagacgat
    43501 caccacgacg gcgcagacga tgccgagcac ggcggccgcg ctgccggcga ccgacagcct
    43561 cgcgagctgc gcgcgcgccg cgcacagcag gccctggccg gcgaggccta cccagatcag
    43621 gaagaacgaa gtgcgcgcga gatcggtcca gaaatcgagc accgaggcat cgcgaccgag
    43681 tgtgactatg agcgccgtca gcacgatgac cagcaccgtt gcgagcacgg tgcggcccgc
    43741 gcagaagtcg ggcaggtagt gcgtgtgctg cggctgggct tcgctcatgc gtccttgcgc
    43801 gcggcgcgct cgaacagcgc cgccttcagg cgatagtcgg cggagatgtc ctcgtcgccg
    43861 tagccctggc ggatcaactc ggcgtactcg gcgagcatgc tctcgacgac cggcagctcg
    43921 acgccgcatt cggccgccat cgcgcgacag atgccgaggt ccttggcgtg caggcgcacc
    43981 ttgaagccgg ccgggaaact gccgcggatc atgttcggcg cgcgattgac gaagtaccag
    44041 ctcgagcccg cgcccttgcc gagcgtctcg acgagcgcgt cgagcggcag gtcctgcgcg
    44101 cgcgcgaacg ccatcgcctc ggcgaccgcg cggatgatgc cggcacacat gatctggttg
    44161 gtggccttgg ccgcctggcc cgcgccaagg gcgccgaagc gcgtgatcgt gcggcccatg
    44221 gcgcgcagca ggggcccggc gcgctcgaaa gcggctgcat cgccgccgca catcactgcg
    44281 agcgtgccgt cgcgcgcgcc ttcgacacca ccgctcaccg ggcagtcgag gaaatcgacg
    44341 tcgcgcgccg ccagcactgc gccggcctcg cgcgcggccg ctgcgcttgc ggtcgagcag
    44401 tcgatcacga gcgcgccggg acgtaatgcc ggggcgattc gcctgacgac ttcgagcacg
    44461 tcggcgtcgg ccgagacgca caacacgacg gcatcgaccg cggcggcgag ttcggcgggg
    44521 gaggcggcag cagcgacgcc gttatcgcgg gcgaaagcct cggccgtcgc gggcgtgcgg
    44581 ttccacaccg cgacgagatg tcctgccttc agtaggttgc gggccatgtg ctggcccatg
    44641 gcgccgagac cgatgaaacc tgcacgcatg ccttgatcgc tccggggctg gtgcggcgcg
    44701 ggtcgccgcg ggccgcaact ttagcagcct gcgcgcgagc ctgtcggagg cgggcggact
    44761 agcggctcgc gcgcggggct ggtccgcagg cggcatccat ggccgcgcga ttagtgacgc
    44821 ggacctggtc ggcctcggcg tccgtaaggt attcacgctc gccattctcg agcgtgcgga
    44881 aaatgcggcg ggcctgcagt gacttctcgt agcgggcctt cgcctcggtg cactcctcgg
    44941 cacgcttgcg cgcgaggtcc tgcgccaccg cctgcccggc cgcgcgcgtc gcctgttcgg
    45001 gactcaccgg cggcgccaca tagggcgtcg ccgcgacccg gctgggcgcg gcatcggacg
    45061 ggctcggcgg gcgtggactg gcggtcttca cgagcacggc gccctccacc ggacgatcgc
    45121 tgtagtgcac ttcgccgcgc gagtcgaccc agcggtagac atcggcctgc gcgccgagcg
    45181 gggccaggag gaaggcgagg gcaatgaggg cgagcgggcg catcggtggg gccttcatat
    45241 ctctagtatg gcggctcggg cacgaccgaa gtttgaaatc ggtcacagta cgcgggcggg
    45301 gggccgcccg cgccctgccc ggggccctga gattagggcc tcgggggtca aagcgcgagg
    45361 ccccgggagg cgcgaggcct cccgtgcgcg cgctgcgcca gcctcgcgcc cgtggccccg
    45421 ggcgccgggc ctacccctgg taagcgcgct ccccgtggta cgtggtatcc agcccctccc
    45481 gctcctgctc ctccgtcacg cgcaggccca ccgtcaaatc ggcgatcttg taggcgatga
    45541 aggcgacgat gccggaccac acgatcgtga tcagcacgcc gagcaactgc gtccacacct
    45601 gcgcacccat gctgtagccg tcgatgcccg taccgccgaa ggtgggcgag acgagcacgc
    45661 ccgtcaggat cgcgccgacg atgccaccga cggcgtgcac gccgaacacg tcgagcgagt
    45721 cgtcatagcc cgcccacacc ttgagcctgg cgaccgccca caggcaggcg cagcccgcga
    45781 tgagcccgag caccagcgcg ccgccgacgc cgaggaagcc gcaggagggc gtgatcgcga
    45841 cgaggcccgc gacggcgccc gaggcggcgc cgagcatcgt cggcttgccg cggaagatcc
    45901 actcaccggc gctccaggcg aggatcgccg cggcggtggc gaccagggtg ttgatgaaca
    45961 cgaggccggt gaatgcattc gcctcgaggt tcgagccgac gttgaagccg aaccagccga
    46021 cccacaggag cgacgcgccg atcatcacca gcggcaggtt gtgcggggcc atcggcgccg
    46081 tgccgtagcc gagccgcttg ccgaccacca gcgagccgac gaggcccgcg atgcccgcgt
    46141 tgatgtgcac gacggtgccg cccgcgaaat cgagcgcgcc gcgctggaac atgaatccct
    46201 ccccgtacca caccatgcgc gcgatcggca ggtaggcggc ggtgaaccag atcaccgaga
    46261 agaccagcac cgccgcgaat cgcacgcgtt cggcgaacga gccgacgatc aggcaggtag
    46321 tgatcgccgc gaacgtcgac tggaaggcga cgaacacgag ttcggggatc acgacgccgt
    46381 cggtgaaggt cgccgcagtg ctgtcgggcg tgatgccggc gagcagcgcg cggctgaagc
    46441 cgccgatcca cgcgccctca ccggtgaagg cgaggctgta accgtagagg acccaaagca
    46501 ccacgaccag cgagaagacc acgaagacct gcatcagcac cgagagcatg tttttggcgc
    46561 gcaccatgcc gccgtagaac agcgcgagtc cgggcgccac catcagcagc accaggatcg
    46621 tggacaacat catccaggtg gtgtcggcct tgtcgggcac gggcgccgcg agctcctggc
    46681 cgagcgcgat caatggcgca acggcgagca gcagggcggt ggcgagtgag gttcgtaact
    46741 tgcgcatgcg tcgactccgt tggcttcggc tggattcagg ttgcggcggc gccggtctcg
    46801 ccggtgcgaa tgcgcaccgc ttcttcgagc ggtgcgacga aaactttgcc gtcgccgacc
    46861 ttgccggtgc gcgcgacatt ggcgatggct tccacgaccg gctcgacgat gtcctcgccg
    46921 accgcgatct cgacgcgcgt cttcggcacg acctcgacgc ggtacccggc gctgcggtag
    46981 tgctcgaggt ggccgcgctg gcggccgtgg cccatcactt cggtcaccgt cagtccctgg
    47041 acgcccagcc gcgcgacggc gagctgcagt tcgtcgagct tgtgggagcg tatgagggcc
    47101 gtgatcatct tcatcgtgtt tccggtgcgt ttggcgagga atgcacgctg acccccgtgg
    47161 cctgcacgcg tatccttgcc gtgtccgcgc cagtcggagt gcagttttcg tgccagtcgc
    47221 ttcaggcacg aaaatgcgcc gctggcgcgt gtttggccgt cgccgctgca ccgtattgga
    47281 gctggccggc tggtccgtgc gtcaattgag ggcaaacatg gaacgcattc cgatcgacga
    47341 actcgcgagg cgcctgctcg agtcgctccc cgccggggtg cgcacgctgc agcgcgacct
    47401 ggaaaccaat ttccgcgccg tgctgcgcgc caacctcgca cggctcgatc tcgtcgcgcg
    47461 cgacgaattc gatgcgcagg cgcgcgtgct cgagcgcacc cgtgcccggc tcgaagcgct
    47521 cgaggcacag gtcgccgcac tggagcgtgc gcgcgactcc ggtgcgagcg actgaggtca
    47581 cgcaggttgc gcgcgtcgcg agccgcgcgc aggtcgggct gaccgcgccc gtcgtcgatg
    47641 tcgaggtgca cctcgggccc ggcctgccca atttcaccat cgtcggtctg cccgccgcgg
    47701 cggtgcgcga gagccgcgag cgcgtgcgct cggcgcttgc gtgctgcggc ttcgaactgc
    47761 cggccggtcg catcaccgtc aacctggcgc cggccgacct gccgaaggaa ggtggccgct
    47821 tcgacctctc gatcgcggtc ggcatcctga tcgcctcgcg ccagttgcgg gtcgcgcagg
    47881 cgctcggtgg ctgcgaactc tacggcgagc tcggtctcgc cggcgaactg cgacccgtgc
    47941 cgggtctgct gcccgcggcg gcgcatgccg cggccgcggg ccaccaggtc atcgtgccgg
    48001 cggccaatgc ctgggaagcc gcgatggcgg cgccgcaggt cgcgggcgcc gcgcacctta
    48061 gcgaggtctg tgcctacctg gccggcgatg cggccgcgtg cgcacgcacg gcgcacgcgc
    48121 gacccgcgag tgcctgccgc gcgccgcgcg agtatcccga tctgcgcgac gtgcgcggcc
    48181 agcgcgccgc caagcgcgcg ctgacgattg ccgccgccgg gtcacacagc ctgttgcttg
    48241 tgggcccgcc gggtagcggc aagacgatgc tcgcccagcg tctgccgggc ctgctgccac
    48301 cgctcgatac cgccgaggcc ctcgaagtcg cgaccatcgc gtcggtcggc gggcgcggct
    48361 tcgaccatgg gaactggggt tgccgaccat ttcgcagccc gcatcacacg gcatcggcgc
    48421 atgcgatcgt cggcggcgga ccgcgggcgc gccccggtga ggtcaccctg gcgcaccttg
    48481 gcgtgctgtt cctcgatgag ctgcccgagt tcgaccgccg cgtcctcgag ggcctgcgcg
    48541 aaccgctgga gaacggcagc gtcgcgatct cgcgcgcggc gctcgatgtg cactatccgg
    48601 cgcgcttcca gttcgttgca gcgatgaatc cctgcccttg cggttaccac ggcgacgagt
    48661 ccgggcgctg ctgctgttcc ccgaccgcga tcgcgaaata ccgcggtcgc ctgtccgggc
    48721 cgctgctcga tcgcctcgac ctgcgcgtcg aagtgccgcg cgtcgccgat cccctatgcg
    48781 atgggccgcc ggccgacgaa gcggcgagcg ccgcgctgtc ccggtgcgtc agtgccgcga
    48841 gggcgcgcca gatggagcgg gcgggaaagt tgaatgcctg gctcaccgtg cgcgagcttg
    48901 ccgggcactg cgcgcttgca ccggcgggtc atgcactgct cgttgcgagc agccgcacgc
    48961 tgatgctctc ggcacgcagc cagcaccgca ttctgagggt cgcgcgcacg atcgcgg
//
LOCUS       DAOQVY010000003        47231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_862_length_47231_cov_6.158937, whole genome shotgun sequence.
ACCESSION   DAOQVY010000003 DAOQVY010000000
VERSION     DAOQVY010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 47231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47231
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_862_length_47231_cov_6.158937"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1879
                     /locus_tag="PKN91_00785"
     CDS             <1..1879
                     /locus_tag="PKN91_00785"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNS26632.1"
                     /translation="RLELLEREQQEIGARATALADELAQREAALAARDAAYAELQQRF
                     ESGDESAGRARAQLAAVQGELTQLREELASTHEQLAAARGDAAGAREALAERERREAS
                     RSAEVAELRAALAAHRERASTREGQRGVYESLLLDAESDVANREARAAALESELAQAR
                     GHIASLETELANAGTRITALMAERDAVNARLHDSEAAIAARAAAQAAGEREAEARLAE
                     LAARDATIAALEAERDRQVETLAERARDIDAGVVERDALRQAVATLEEALARRAQQID
                     EQQAENERLATAVINLTRELQGAQTTYSSQRDSVSRQDERLENLDRLLRAAEARDADH
                     EATMRAATALHDELEAMLAGEKQRNAALAEELAALQAAVAERDTHVSSAGEALRSHTE
                     RSRELERRERELIEQLEERAGALDAARAELLEATGRVAELQQDLQAAEEQVNRVEAEL
                     RAKQVRLDELGAEATQTRAQLAATRRALEERDALIGRIEAETASSAAVLGSIRESMQR
                     LDPLAVTGNEATADGLARLLTRLDGPGEVVHVLGRKTTIGRTPDNDLQINAKFISRHH
                     AVILAGPTLTIVEDLDSTNGVYVNGRRVMREALTEGDLVMIGKTKFRYSVRPVGDRNP
                     G"
     gene            1950..3467
                     /gene="ilvA"
                     /locus_tag="PKN91_00790"
     CDS             1950..3467
                     /gene="ilvA"
                     /locus_tag="PKN91_00790"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HNS26633.1"
                     /translation="MASPYIERILKARVYDVAIESPLEHAPRLSRRIGNEVALKREDL
                     QPVFSFKLRGAYNKIANLSQSAAARGVICASAGNHAQGVALAARRRGANAVVVMPVTT
                     PHIKVQAVIDLGAETVLHGDDYDAAYEHALELARERGLVFVHPFDDPDVIAGQGTVAV
                     EVLRQAGGDIDAIFVPVGGGGLIGGIAVYVKYLYPRIKVIGVEPVDSAAMHDSLRAGS
                     RVALERVGMFADGVAVRRVGEETFQLARDFVDEIVLVDTDETCAAIQDIFEDTRSIAE
                     PAGALAVAGIKKFAARAGWRGKRLVAIISGANMNFDRLRFVAERADLGAQREALLAVE
                     IPEQPGSFLAFCQALGPRAITEFNYRYESAHAAQIFVGFALSQGREEKDAVVAALRAA
                     GYLVQDMTDNEVAKLHARYMVGGHARGIEHELVFRFEFPERPGALLRFLQAIGTRWNI
                     SLFHYRNHGSDYGRVLAGIQVPPAEREDFLLHLAELHYEYVDESDNPVYRMFLGA"
     gene            3464..4372
                     /locus_tag="PKN91_00795"
     CDS             3464..4372
                     /locus_tag="PKN91_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNS26634.1"
                     /translation="MNDIDAAYVPRRVPVHEDLVLRGLRHRITRWGPPSRSPIVLLHG
                     FLDAADSWQFLVDELPEDWSFVGLDWRGFGGSAWQQDGYWFPDYLADLEALLAVVADA
                     APVRVIGHSMGGNVAALYDGIRPGRLRWIVNLEGFGLADTKPDDAPARYARWLDELAK
                     PPRQSTYASPEDLAKFLRTRNARLTRARAAFLARAWVREDGRRWRLAADPRHRLVNPV
                     LYRREEALACWRRGTAPMLLLLGALTEYRARLGEHGSDGHLRASFPNAMIDTLAGVGH
                     MMHHEDPAAVARAIERFARIQVPDVS"
     gene            4369..4635
                     /locus_tag="PKN91_00800"
     CDS             4369..4635
                     /locus_tag="PKN91_00800"
                     /inference="COORDINATES: protein motif:HMM:NF021193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HNS26635.1"
                     /translation="MNWKWPDLHRDHPLHLPYRWMKRIVITLTGCTVLAVGLAMTVLP
                     GPAFIVIPAGLAILGLEFAWARHTLQAIRKRGALIVEAARSKLS"
     gene            complement(4632..5120)
                     /locus_tag="PKN91_00805"
     CDS             complement(4632..5120)
                     /locus_tag="PKN91_00805"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNS26636.1"
                     /translation="MRRRDFLAACGALIAQQMTHADDRGIALVSAGGLRSALDAERGK
                     VVVLNLWATWCVPCLREIPDLVRIERELAPQVVLIGVAMDEPDERESRVAPFHAKYFP
                     DFRSYLRSERELDAIASVVDPAWNEVLPTTYLLGRDGRVVRRIQGAKSHEEFRALIAA
                     AL"
     gene            complement(5120..6442)
                     /locus_tag="PKN91_00810"
     CDS             complement(5120..6442)
                     /locus_tag="PKN91_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26637.1"
                     /translation="MPNQTTIALAVLAALALVAPAAAAPPATTPLALSTDEQLALAPD
                     VAVGPGGEIAVLWLAKGNPESAAAKAAEADRAKHGHTHLSSMNLYVAVSKDGGASFGA
                     PLRVNREDNEVWGFAVSRPRVAYGPGGTLHIAYPANETQPQLGKPVLTARYTRSTDGG
                     ASFEVPRRLSTVTDADLAQVIHGGFASVAAFGTMGVAPNGDVHLVWIDTRDMKSGADN
                     ASIYGVVSHDDGRTFTQDRVEAAGGVCPCCQLSLAFDADSRPWLGSRLVTPDGVRAST
                     VARADGVDGSFGTRVSTGGAPWKIDGCPLKPTVLAVRGDTVYTAVHNGAEDPPGVLLA
                     VSRNGGASFEAAYPAHPGAQVSDAPAIALAGGDRAVVAWHAKADGPRRIFYRTLDAAG
                     QPLDAPSTLTSGDETAQHPALASRHDGRVQIAWQQGDLIYTAIIGPAE"
     gene            6514..7560
                     /locus_tag="PKN91_00815"
     CDS             6514..7560
                     /locus_tag="PKN91_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNS26638.1"
                     /translation="MEDIASAPWLRRLIVAALLAGLIVLAFEVLKPFIVPVIWAVILA
                     HVTWPLYRRVLHAMKQRRAPAALLMTVAVTLALVVPAIWLAELIRTELVAAYGAFAER
                     LAQGVTLPRFLLDLPWAGPWLTELLARIADDPGALNVEVLTWLERSAGEIGGVIGGVG
                     RNIVKLAIAMLSLFFMYRDGESFAAQVTRVLEQSLGQRVHGYMLAISQTVRAVVYGLL
                     AAALAQGTLAGLGYWVAGLNAPIALGALTMIAALIPFLTPVVWVGASVWLLITNHAVA
                     GLGLFLWGLLAVSWIDNFVRPLVISNATRIPFLLVMFGVLGGVLAFGLVGLFVGPVIL
                     AVLVAVWREWLTDK"
     gene            complement(7573..9267)
                     /locus_tag="PKN91_00820"
     CDS             complement(7573..9267)
                     /locus_tag="PKN91_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNS26639.1"
                     /translation="MASVRITRHFATIEQGRWGRRQVHYRRTGKGPLLILLHQSPLSS
                     RDLVPTMQRWHRHFTCIAPDTPGYGQSDTLGVESAEMGDFADAVIEFLDAIKVRKAAF
                     YGYHTGAMMAAQVAAKYPERVHAVAANGYVIPTREERDDLLANYLPPFRPTWDGGHLA
                     WLWSRMREQVIFFPWYAKGAADRMHVNLPSPASLQAGLVEMLRAGDNYRVAYRAAFAM
                     PSAEVLQQIRVPALITAASTDPTSRYLVRATDPPRNVTVRECATALETLELCLAHLRK
                     ARPSAAPKLAPTRPIAGRLWQQSLDVRGGQLRIRRNTDAPGRTVVVLHDAAGSSDTVA
                     ALAASFIGRRPVLAIDLPGHGESDSTLPGGTVTVAAYARALRGALDALGIRECDIVGV
                     WGGGLVGLELAHLAPRRVRRLVMVGPLWFDRALQAELRQRYAPDLQVDAHGGHLLRAW
                     HFLRDQGLFWPWYNTTREGIIRGEPNVDTQMVQRRVLEVMRAPAWRAAYRAHFSYPVK
                     ARLRDVKVATLLAAADWDPMREHALAAAQTHAQLPFCALARDPAEWGPTLLRFLDA"
     gene            9356..10615
                     /locus_tag="PKN91_00825"
     CDS             9356..10615
                     /locus_tag="PKN91_00825"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS26640.1"
                     /translation="MQRNAAAYMSVRRPAALRALRHRDFARFAAARFCATLGWQMLSV
                     AVGWQVYEFTGDPLALGLVGLAQFLPFVALVLPAGQIADRADRRLILVLSYAVEALCA
                     LALFALALLDLRITWPIFVAMLLFGAARAFWMPAGQAMTPNLVPAEDFSAAVAVNSAL
                     FQVSVIAGPAIGGLLFLAGAEIVYGSVLLLLVLVVVLMLRIRPVRVAGSGDGYHLRDA
                     LEGLRFVIRNKPLLGAISLDLFAVLLGGATALLPVYASDVLHIGPAGLGILRTAPGVG
                     AAVVAAWLAVAPLSRHVGRWMFFGTGLFAFATVVFGLSTSFALSLVALVLLGAGDMLS
                     VFIRHILVQLETPDSIRGRVSAVNAMFIGTSNELGEFRAGVAARYLGAVPAVVWGGVA
                     CFGVVLAYTALLPTLRRLDRFPWPNRG"
     gene            10723..11868
                     /locus_tag="PKN91_00830"
     CDS             10723..11868
                     /locus_tag="PKN91_00830"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNS26641.1"
                     /translation="MRLPHSPTPRVPRDQRRRVLLGIATLPLAPLAVARARSSGFSLA
                     LIGQCLIRHDLCAQPWPGLAPLARRLRANDACFSDLEVAIRGPREGAPTRDSGTLHTA
                     DPVVLDCLRAFGVSFLATSNNHAFDIGTGGILDTIDALRARQFAFAGSGRTLAEAAAP
                     AYQLTAAGRLAVVAAAAGKIRDGGAATDARPGVHELRSAEGGLDAGDVERMLESLRLA
                     AREADVVIAYLHNHLWAEEISDTPDWQRSFARRCVDAGASVFVAHGTPLLQGLELYRG
                     APLFHGLGSFIFQTRKPVGAYGEPNWESLVAECRFERGRLIEAQLTPVALAPVGIGGP
                     DDYDTRGRPSPADASQARETLGRVAGLSQRLGYRLRHDGARATLRAG"
     gene            complement(11894..13738)
                     /locus_tag="PKN91_00835"
     CDS             complement(11894..13738)
                     /locus_tag="PKN91_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNS26642.1"
                     /translation="MSAEPGIRRRTERVAMRDGVHLATDIYLPQGTGPWPALLERTPY
                     GRRGTNHADRSLADPVPKSKPEIAMQFARAGYACVLQDCRGRFDSEGEFTKYLNEQPD
                     GIDTLAWIAAQPWCDRRIGTLGFSYGAHVQTALAAAAPAGLGAMFIDSGGFSSAFHSG
                     IRQGGAFELKQLTWALKHARLSPATAADPVRRAALEAADIRTWARVMPWTRDHSAVSA
                     APEYEAYVIEQWSQECFGAFWERPGLYARGSYDRFADVPMVHMSSWYDPYALTATENF
                     VALSKLKRGPVRLVMGPWTHGQRSVSHAGAVDFGPSATLDGNVAPDYMTLRRDFFDRH
                     LLGRDAPDWLAAPVTVFVMGGGAGTRTPQGRLLHGGAWLRTADWPPPEARPAHWYLHA
                     GGVLATQQPCHDEVIAWRFDPRDPVPTIGGAIASGAPLMEGGAFDQRETPGIYGAKHP
                     GRALAARADVQVFETEPLVDDVIVAGPVRARLWVSTSALDTDFTVKLLDVHPPSPDWP
                     DGYAMNLTDGIRRLRFREGYDRALPAEPGRVYAIDVELFPTANRFVAGHRIRIDVSSS
                     NFPHFDINPNTGAPAGVPSEPVVADNCLHLGPRGPSHVTLHVLPVRGA"
     gene            complement(13735..15270)
                     /locus_tag="PKN91_00840"
     CDS             complement(13735..15270)
                     /locus_tag="PKN91_00840"
                     /inference="COORDINATES: protein motif:HMM:NF015746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HNS26643.1"
                     /translation="MRVVLDRIERLGNALPDPVVIFLWLIAALIVASAIAASLGVAAV
                     HPLTGERIAAESLLSEANVRRLLVEMPQTFVRFPPLGLVVVVMLGAAVAERSGLLATL
                     VGRAVSAAPRAWLTPATFLIALLSHHASDAAYVVLIPLAAIVYREAGRHPLLGMAVAY
                     AGISGAFAGNLLPGQFDALMLGITQSAAQLLEPTRGLNPLGNWWFTAALGGLLFCVAW
                     PVAERWVAPRLAAVVVDGEALEVIGVDSVPTVPSEAQRRGLRRAGVAALLVIAVFAAL
                     VLWPGYTPLLDPDSEGYARKAPFYSALIAGFLLLFLACGWVYGRAVGSITGHRDVVRM
                     MVDGLRDIAPYLVIAFAASHFIAMFAWSNLGPILAIHGANWLRGLALSPVAMLVPLLL
                     VSSVFDLLIGSASAKWSAMAPVVVPMLMLLGISPEMTTAAYRVGDSIFNIVTPVAANF
                     VLVLVYCQRWVRGFGVGSLISMMLPFSAAFGIAGLLLVALWAGLGLPVGPGAPAIHLP
                     AVP"
     gene            15446..16345
                     /locus_tag="PKN91_00845"
     CDS             15446..16345
                     /locus_tag="PKN91_00845"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNS26644.1"
                     /translation="MKISLRGLESFREIMRKGSATAAARELSLSQPAVSRLIGQLERE
                     LGFNVFDRIKGRLVPTQQGLLLYEEVESAFLGLERVSTVARDVHDLNAGQLRIVAPPS
                     LAEGPLVGIIGTFIKKYPNVRVVLDSNSRPTAMNMIATRAADCGFGKLPLEHPGIHKR
                     PLVVNETACVVPRGHRLARKPTVSATDLASEELIMIGRGSDTRLRIEAALRAAGVVPR
                     IRLETHNVGAACAFVAAGVGITLVNDMLARAYCKREIELRPFRPRIIHEYIFMTSSGA
                     SASPLTLAFYAACRDALHRYHAA"
     gene            complement(16417..19089)
                     /locus_tag="PKN91_00850"
     CDS             complement(16417..19089)
                     /locus_tag="PKN91_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS26645.1"
                     /translation="MLPLAVCAQDVASNAIEEVTVTGSRIARADAESVGPLTTLTAED
                     ITFAAALSVGELLQELPGAGVSLNSNGTQGTSFGVSSINLRYLGSAEGSGNRVLVLVD
                     GHRWVNAAGGRTFRDFVDLNTIPMGLVDSIEVLKDGASAIYGADAIAGVVNIRTRRSI
                     EGIEARLNYGQTSHSDGESLSADLSMGHSFGRVSALLSTSYVNTDPVLTMDRSLTAVA
                     AVAPTNPPSSPRGLYLLPGVATRAVTRIPDTNGDDPADFRVATLPDDYYNTQAQGVYA
                     TGPSERFGVFGRIGVEFSDSLSGHIELLYNERKSNQLFSPLLLDLRGSYSITIPADHP
                     YNPWQTAFTTNSLRLQRMPVEVGNRNNVQDVETQRIAFGLDGEFELAGLWNWDLFASH
                     AKNDATFNAYNQADQDKIALALGPNDRCAANNCVPLNIFGVMTPQMADYIRYTARNDN
                     GTEQTDVAFNISGSLVDLPAGRVGVAAGVEWREESAYDDPDAYVNSAPEFITNETRTT
                     SSTREPTRGKYDLWEAYAELAVPLLADLPGARALDLSAAVRHSDYSSFGNATTSKLGL
                     AWRPVDDLLIRGTFSEGFRAPSILELYQGQRQTTFQAVDPCNGGGAGLPGCAGVPATY
                     NQNQFGSGTLRGTIGGNPDLKPETADTYSVGFAYTPGWAAGMSLTADWYRIEIDEAIA
                     SQSAQQLLTLCAQRAGAYCNLVDRDLSTGEVRDLRQVVLNFSSIEVEGLDVTWRYGFD
                     TPIGRLNAVLDASRLFHFTNFVPQTDGTVQRTELAGTGDTPRSTYPKWKGSAGLRWEQ
                     GPWSGGYKARYIGSSTDVAGNTLNGGRTDDVVYHDLQLGHHFERFNSNITLGIDNVLD
                     EMPPISRANNPMNFDIYTYDVRGRYYFIRLMTRM"
     gene            19556..20821
                     /locus_tag="PKN91_00855"
     CDS             19556..20821
                     /locus_tag="PKN91_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNS26646.1"
                     /translation="MSAAYAGEVPAPTALIHAGHLLDRPGSQPRSSATVLVRDGRIAE
                     VRDGFVDPQDFPGAQVIDMRAYFVLPGLIDSHVHFNGTESGKGALLERLSKDTAFFAY
                     RAATLARLTLDAGFTTVRVLGNPAGVTLSLRDAIAAGALPGPRILDAGNSIAATGGSG
                     DVTLGVHSALAGHMDQDNLCDGTESCRRAVRLQVRRGVDLIKLKITGGVNSRVATGLG
                     RQMFIDEARAVVETAHLYGKKVAVHAHGADGINLALECGADSIEHGTLLDDQGIELML
                     RSGAFLVPTLSTATTYVERLANNPGTYTQEVREKMEWRASVTGQALRQAYARGVRIAF
                     GTDAGVSPHGRNAEEFVLMVKYGMTPGDAIKAATLNAAELLGIADEVGSLEPGKRADL
                     VAVRGNPLEDIAVLQHVPFVMKGGLIWKQ"
     gene            20902..23004
                     /locus_tag="PKN91_00860"
     CDS             20902..23004
                     /locus_tag="PKN91_00860"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HNS26647.1"
                     /translation="MAGWLKGRGAVSNPAPRFERLHELGVDDGWYTEPAPDSIATLVQ
                     PEPARSVISRNDSPDIGFSQSINPYRGCEHGCVYCLAGDTAITMADGSAKPLASLERG
                     DEIIGTVRRGHYRRYVATRVLAHWRTAKPAWRVTLADGTRVIASGDHRFLTERGWKFV
                     AASGSSHARLTLNNSLLGFGHVAQVDRCETESAKRGYLTGVIRGDGHLKVYSYRREGR
                     ASGDQHRFRLAMIDSEPLARAASWLGEFGAPTARYEFGTATAGRQAMGAIRCSSRASV
                     ALITHLVRWPETADRDWQRGFVGGIFDAEGSFSDGIIRIGNTDPRIISELITALCAFG
                     FDTVLESIPRRDRKPMQYVRLRGGLAQNLRFFRLFDPCIARKRNIAGAAVKSRADLRV
                     VAIEPLPGTRELFDITTGTGDFLANGVVSHNCYARPSHAYMGLSPGLDFETRLFYKAG
                     AAARLTEELSRPRYQPKTIMLGANTDPYQPLEKRLRVTRSILEVLAEFHHPVAIVTKG
                     ALIERDLDILAALASERLVSVMVSLTTLDVDLKRRLEPRAASPAARLAAIRHLRAAGV
                     PVGTLIAPVIPGLTDHELERLLGAAAEAGARCAGYVVLRLPHELKDMFRDWLAEHYPD
                     RARHVMSRVHSIRGGRDNDPQFGSRMRGTGADAQLLRQRFDVALRRLGLERRMGFELD
                     TTRFRRPDARGRQMPLAL"
     gene            23149..23784
                     /locus_tag="PKN91_00865"
     CDS             23149..23784
                     /locus_tag="PKN91_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26648.1"
                     /translation="MQARVEESRELPTRISGDEGVVVLAKPQVEGAGTEAGFMDCVGA
                     NLAGGTGGLRVHGNDEFVDAMFPWFEPSTAPSRPEAVSALLAHPGVSERVAASGVRYI
                     VWVDGATRRTDGGGSLACGAAPGAAGCIGFGWWEKESDYEATIWDLQQAKSAGSVGTN
                     VTGTSAIVGAVIPLPFIARVQGTACNRLATQLRSFFAGDGLAEPVGTDDAR"
     gene            23788..24624
                     /locus_tag="PKN91_00870"
     CDS             23788..24624
                     /locus_tag="PKN91_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26649.1"
                     /translation="MRLSQGWIPVLVAALLLGGCATQQSGGSSGGGSSGGGSSDGGSS
                     GSSMPSGGGSTGSGMPSGDGSTGGGMSMPSGGVPDTGQPGTPGTAGDAAGQPDGSGEQ
                     EGATGAAGSEGAAGTGPELPSGTAQTPEERRAAVDRRLDDSLGTFDERIRKEQEGVAK
                     ERESNAASGAGAGVLVAQTGSESGGRPGDLKSDRDSASTQGGSQGSQGGQAGAGSEGP
                     ARTSAGTGGSGKAAVERADGSDDDIVARRLRRAAEQETDPELKEKLWKEYDEYKRSVR
                     GG"
     gene            24633..>24907
                     /locus_tag="PKN91_00875"
     CDS             24633..>24907
                     /locus_tag="PKN91_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26650.1"
                     /translation="MHDVVRLLRQAGALALLAALAACVTQETRPLPKLNPVQATEQIP
                     EAELLDVGIRAFDANIPEQYKDDEEELARRRIFPEVRQAESAYLPGLL"
     assembly_gap    24908..24917
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <24918..26008
                     /locus_tag="PKN91_00880"
     CDS             <24918..26008
                     /locus_tag="PKN91_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26651.1"
                     /translation="DRRVEAIVPILNPTVHEQILDQIMVANLADNRQSWTVLPDGTSV
                     RVVPPNATVDVVVDGRIIGSTGARLALVVTVTDSMGRVWIKDKAYEGEADLGSYKTDA
                     ALQARDPFQNVYSQIANDMLAARAALTSADLREIRRVTELRFAGEFAPQAVQGYLSTD
                     KQGISRVARLPAEGDPLMERIDRIRERDLSVIDTFDGYHASFTDKVESTYGNFRRVSQ
                     EEIEKEDRARASARTRTVLGAAAVLASVLIPDQCSGGDYTCRNVQSAARTAGAVGGVA
                     GVMSGIKKYSEAKVHAEALKELADTFRAEAAPLVVEVEGRTLKLTGTAEEQYREWRAL
                     LHQIYLEETGGVVAGAPPQDSPAVTPSE"
     gene            26071..28455
                     /locus_tag="PKN91_00885"
     CDS             26071..28455
                     /locus_tag="PKN91_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNS26652.1"
                     /translation="MAKIGVGGHSEVWRARDLVRREDLALKVMKRIAAAPADAWALLQ
                     HEYAVTRELGHPGILRVYEPLDCDGTLVLPMALAPGGDLGALRGEPYTRIVPALIGIA
                     QALEHAHGRGVVHRDLKPGNVLLDAEGHTLIADFGVASFSGGSPFADHGSPFSASPQA
                     LRGEPPTPSDDIYGLGTLAYELLSGYPPFYPRFETRRVLEEPVADLRPIQPAPPRLVA
                     LVMRMLAKSREARPESMRAVIDELHASLHDTVTIETEALAGSGNGTEVIAASNPTTPI
                     AGSPGDASGDDRTMAIELPEPMRALALPTSGSADEIVAAPHDAPALIEIEPAAAPRER
                     RIGTWLALAAVAAAIVLVFFVMPRLAPRTAAPSAGVDAQDAPAEALDPATPAADPVEA
                     LATLRAGITKRLAAVETRAAAVWGGDDFAAAKQAAEHAGVLADDGEIDAAVTEFTALG
                     KRLADIEARAPDALAAELGRGDAALEAGEPARARNAFELAQRIDPQNARAAAGLARAQ
                     ALEIAMPLLAQAANAAASARHAEAAELYQQALAADPANTQAREGLAAARAAVGAGEYA
                     RTLASGFSALQAGDLGRARSEFERARAMQPSASAPQQGLEQVAAAEQARAGDEARRRA
                     ASLEASERWGEALAIYEEVLARDPALQFAQAGRDRAAPRAELARRLQLIIDNPDRLAA
                     DAVRRETEELLELARAIEPGGPVIRSQVSRIELLLPAYDQVVRVALESDNATEVTIQR
                     VGAFGTFLRREIELKPGRYVVTGRRDGYRDIRREITVAPGTTTQTIAVSCIEPI"
     gene            28437..30866
                     /locus_tag="PKN91_00890"
     CDS             28437..30866
                     /locus_tag="PKN91_00890"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNS26653.1"
                     /translation="MHRADLMETERISVTEPLGQRELAAPLSIGAAGSDIVVPGTDAT
                     PALVLDVDEGLWRVTPAQDAGARLNGERLREARDLRAGDVITLADAQVIVRATAPCAI
                     EVLHLAGNDTVAPLVVAEERDGALDDEDIEIGAVAREVLALDDEAPRAAGGRIARRVA
                     IAAALAVVAAVFALFASVQRVPLVLDPVHARVGATDTLIDWHSGATLFVLPGKHQLRA
                     SAPGYVAVEREVTVTREGAVPVRLRLAKEPGVLVIDTGGVMASVAVDGAEIGRVPGEV
                     RVAAGNRTLTLCAERYLDAVVEVEVEGLGVRQDLTVAMQPSWGTLALTVNTPGAMLAI
                     DGAAPAAVEPRIELPAGVHQLAISAAGAKSWQSAIVIKAGETTTIGPIDLGAPDARLV
                     VRSVPSGADVSLDGVYRGRTPLDIAVAPGSMHDVLVARTGHAPWSRSFSAASGERTVL
                     DARLEPVYVSLTVRGEPADAEVLIDGVSRGRTPLTLDVLAGEHAIEVRKVPLASHESR
                     ITLAPGLARTIEYQLTAAGRPSSALAAGTQLGTRVGYVLRLVQPATFQVGSERREQGR
                     RPNETRRQVTLTRAYYIGATEVTNAQFRRFRPEHASGHVNNTSLDLDAQPVVKVTWED
                     AVQFCNWLSEQEGLTPAYESRDGKWQLKTPVSNGFRLPTEVEWEYAARYAGPGALQRY
                     SWGNALPVAPANGNYAGAEAIGTMSPILETYRDDFPVAAPVGKFGANRLGLYDLSGNV
                     SEWVHDYYVSLPEGAAQTDPFGPAQGTRHAVRGSNWRTATVADLRLAWRDGAEGSSQV
                     IGFRVARYADP"
     gene            30892..31176
                     /locus_tag="PKN91_00895"
     CDS             30892..31176
                     /locus_tag="PKN91_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26654.1"
                     /translation="MRNIWRQIWIISFASLALWAAVLAIAYRLPSAVAQSTEEPAPSA
                     EPAPAGEAPPDEAGMIDEGPQIGAPVTEREEVPPELRQSADNNISFPVDI"
     gene            31192..31995
                     /locus_tag="PKN91_00900"
     CDS             31192..31995
                     /locus_tag="PKN91_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNS26655.1"
                     /translation="MPIQRRYPSEFVFSLFALAITIVIVHGVYVLDIRPKAQAILEEQ
                     RAGMLKDPNYIAERSFYVLIKDYEQESEIILFIWALAIMGYKARHQKRERETLEGDPL
                     KLPEGMKILPEDSREYTRQLEALPSEKRQLLLPRALLAALARFGATRSVQDVSNVAHG
                     TCQSEAERLDSEMSMLRYIAWAIPAIGFIGTVRGIGDALSQAHKAVGGDISGVTEGLG
                     VAFNSTLIALLLSIFLMFLLHQLQLAQERLVLDAETYLDTRLIRNMQAR"
     gene            32003..33397
                     /locus_tag="PKN91_00905"
     CDS             32003..33397
                     /locus_tag="PKN91_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNS26656.1"
                     /translation="MTTLGVSIHDGALALALDGRVLSVAPAIVRRAGGAAPAGTPALD
                     FLRSRPAEASARHWSDIAAEGAAARAALPIALAELRERLREACAVDGAAAGCDAAWFA
                     VGAAFPPSALSGVLGAARAVPLPLRGFVDAAVASVAPLGLTRPAIVLEVGLFHVAATA
                     VECGPTVRRRRSMVSRGGGLLDIYQSWLDVVSAAMVLRTRFDPLHDAAIEQRLFSELP
                     ALAAQAEESGVATVTLEGPGGEPHAVEIARDQLAAAAAPRYRELERCLHELRRAGAPV
                     ALVVPAALLRLAGLRERFESLPDCELVIVPDGWAGAVASMLPVPAEGADAPVRLLRRL
                     DALEAAPAGEAPRRERLATRGGAVDPTHVLHAGRALAPDDRGISVGRDGNDVVISVSS
                     GAAGVSRRHCTFLRGDDGVMLIDHSRHGTWVNDERVAGRARLRAGDEVRIGEPGIVFA
                     LIAVGAESAQASAD"
     gene            33375..34469
                     /locus_tag="PKN91_00910"
     CDS             33375..34469
                     /locus_tag="PKN91_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNS26657.1"
                     /translation="MRKRRQTEVFSLSFLDCICCGFGAVVLFYTVVSAQSGVERIRKT
                     EDLAAEVRKLEDEVLEGTRNLVVLRNTLEKTSSETASAASRATRLIEELKQARDQGPI
                     IDNTTLAQREHIEKLKADLRSLEESTRRLEAASKDVAPPGDAVKVTRGADRRYITGLR
                     LHGKHVLVLLDSSASMLDEDIVNVIRLRNTGDATRRAAAKWRRGVATVSWLAGQIPPG
                     SSYQVYRFDTKPEPVLADTAGNWLKGGDQATGDRLLEALDEVVPQNGTSLVNAFRAIR
                     QLSPAPDQVILITDGLPTQGASPPAVRRYIDAGGRARLFEDAIKALPPRTPIDVVLLP
                     MRGDNPAAHAFWRLARNSGGAYLMPSKDWP"
     gene            34480..35526
                     /locus_tag="PKN91_00915"
     CDS             34480..35526
                     /locus_tag="PKN91_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26658.1"
                     /translation="MKLQRRDTEVFSLSFLDCICCGFGAIILLLVLSEFGEPIVIEKS
                     REQLDSQVLALQQELNVIRGETEVLNREMQARVDELRKERLRLARVSGEVSSIRGQYE
                     ASRKEAAVTNIIEGELVQAYESLSEELKRLQKVSRRRPPTEAVGGIPIDSEWIIFVID
                     TSGSMLSMHWEGAQQVLKEILDIYPRVRGLQVLDDEGKPMFAGTRGQWLQDNPQQRTR
                     IIDKMRGWRAFSDSDPVQGIQEAVATYWAADKRISVYVIGDEFTGDSIQRALDAVDKA
                     NPPDANGRRRVRIHAIGFPEARGMPAFTSIRFAALMRAMCDRNGGTFVPVTNEKSCAR
                     YIEVFGTRQCISEG"
     gene            35508..36284
                     /locus_tag="PKN91_00920"
     CDS             35508..36284
                     /locus_tag="PKN91_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26659.1"
                     /translation="MHQRRLAKVIGPLLVRLAVGAGALLLAGCGSVEIAPEPVIPRPL
                     VRPIAATTAVVLGGEQRNFKHTETRAGVDWLVALGAGHKRLAEEVFGALFPGCVFVDE
                     LEEARGIAGLDAVFEPRMLQYSFATANETGAGYYAVTINYRINIYGPDLKLVDSYTLT
                     GYGNDRDKALSSSEPLAGATRAAMRDAAAKFLVQFPQQPLAQRLASGEPLVAPGDAAP
                     GEEINRADAARQIEAVPVLAPKNEPVSPPVDSQPKVVQLP"
     gene            complement(36296..37669)
                     /locus_tag="PKN91_00925"
     CDS             complement(36296..37669)
                     /locus_tag="PKN91_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS26660.1"
                     /translation="MRGKSALAALLIVACGAALAPRAGAVTDAERTAIYQQFRTLFDA
                     GRYEEALPLAEQLVAATREQYGADDRALAAPLTNLGTTQLRLNQFSAAEASYRRALEI
                     VRSKANATATDRALLRPLQGLGMTYARSDQPAAATTALQAAIDLSRNIDGLYNLEQLE
                     YLFTLIDAYVQDNRLTEAEREHLYAFRIAETAYGRDDPRMLPAYDYLARWYEYVGRYT
                     TARAHHYRALRIAEASSGRGSIATVMPLRGLARAYRLEYLFGAEPVEQSAVNDPLQFS
                     SGPGASQSPSRLNPEGERSLQLALAAIDKAEPPDPALRGETLVDLADWQLIGGNIRGA
                     LLAYREAWQALEEAGTTALVDAPRQLRYRPPSASIARFKGGDVNDYEEFVIEAKFTVK
                     ADGGTADIATVPSVAPQSMHDSVAYAVRKAVYAPRLVNGEPVDTPEVSLVERMLKRRP
                     QVADDTG"
     gene            37725..38417
                     /locus_tag="PKN91_00930"
     CDS             37725..38417
                     /locus_tag="PKN91_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNS26661.1"
                     /translation="MNAVHKTAMVAGASGLVGERLISVLCESGDYARVYAVTRRPLAR
                     DHARLANRIVRFEQLEAQLRGITCTDAYCCLGTTLKAAGSEAAFRHVDFDLVLAFARA
                     AKAAGAQRFVLVSAAGADRGSRSFYLRVKAEAEAAVMAIGFAALDIMQPGLLLGSRRG
                     LRPLELLAQWTLPAIAPLMIGPLQPLRPVSATTLAEAMLGAARSGRRGVMRFTWRAIT
                     TLAAGQASRRAG"
     gene            complement(38418..38666)
                     /locus_tag="PKN91_00935"
     CDS             complement(38418..38666)
                     /locus_tag="PKN91_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26662.1"
                     /translation="MAKRKRTTHRSSKGTKLYAVRDKSGKFKDIQSYKRAHAADLRST
                     AASELPKRKAKKKATKKTAAKKKVAKRPVARRKVAKKK"
     gene            complement(38729..39856)
                     /locus_tag="PKN91_00940"
     CDS             complement(38729..39856)
                     /locus_tag="PKN91_00940"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNS26663.1"
                     /translation="MLAAVTYVSFQRTQSRRRAAWLLALTLVLIFYASFFPFRFDVGR
                     LIAAWGGGPGQLLPWGYSARADRAGNLLSYLPVGALLAYLAPVRWRAPLAFACAVALA
                     GALSVTIEVLQHVTRSRVPNLVDVAMNSSGGALGAIAGALLRSRPWPIGAIALRHARP
                     EPIALVLLALWFAMHAVPFMPRLNQGHAWAGMQPLRDLDYDFIAILTSCAGYLVVASA
                     VRRLVVPDSVMRIFLTVAALSLAARVVFVGQWLTLDECLGLAIALPLAVTLRGVTRAR
                     AFRITAAIVTLAFALHLVLPPALRWGDALIFLERAFMITGMLWLAAGGGVSLLASTAA
                     AAVGLATLAGVPSGLLAVVGGFLVHAGRVLHSTPVARIVER"
     gene            39963..42557
                     /locus_tag="PKN91_00945"
     CDS             39963..42557
                     /locus_tag="PKN91_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNS26664.1"
                     /translation="MDPLRACLGTRMGRRIAVTVAVAVLAPTLLAAFVLLLHSARTMD
                     AAAARALTIDAQAFAAEFGARLDGGRAAANRHDAAVSFAAESAARLPADAVATPIGTE
                     RRIALLDGAGRLLFSTAPLPTDVVDLLANYSRAPAATRGALPLAWQAAGVEWQGGAAR
                     LEPREPGGTSWSVVVFKPRSAWSAAVAPLGGELAALALFALASMVIGTLTLVRRYVPI
                     TMRFAAAQGEGGVGIAPGPRSVDADELGDAIHAGDLATGRLAERVAALETLGEIDRLL
                     LGSAELEQMFDTILSRVRAVTRCDGVAITLIDADSATHGRVYFAAPQVVDLPVVRVEL
                     DPDMLATLSQAQEGITITRCEPERHSLLRPLAELGAQFFWVWPVISGDRLVAALSLGY
                     REPPSADPSVANYGAEFASRLSVALSNSARDERLYRQAHFDALTGLPNRLLFRDRLAQ
                     ELASAVAGGSRGALLYIDLDHFKRVNDTVGHGAGDQLLTIVAQRLRACVKDGDTVARL
                     AGDEFTVVLRQVTDPDAVRTVAERIIAALEAPVNVAGRDHLVRGSIGITLFPDDGNNI
                     DELMRNADTAMYRAKDLGRGRAVFFDRALTVRQVSATGSGLYRALRHREFSLYYQPQF
                     SIDDGRLVGLEALLRWQTPRSGLKYPAEFVPAAEETGLIVDIGGWVLEAACAQIALWR
                     EQGMSPPRLAVNVSALQLKCNGFVANLEQLLARHGLAPGAIELEVTEAVLANPDIAEV
                     LRELAARGIRLALDDFGAGETALAHLRHHPISVVKIDRTFIEEIAHDPTAAALGATVI
                     AMAHALRKSVVAEGVETIEQLDFLREHGCDIAQGYYLARPLSAAGIGELLAGRAIASQ
                     LTRDRLVG"
     gene            42662..43108
                     /locus_tag="PKN91_00950"
     CDS             42662..43108
                     /locus_tag="PKN91_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNS26665.1"
                     /translation="MPASGDKTSPAATAPGRGIVLAAEGVIGTPYRYGGASLQGFDCS
                     GLTQFAHRAAGISIPRTAAAQQRAATAVARNALQPGDLVFFVTGRQGVDHVGVYAGGG
                     RFVHAPSSGRVVAYAYLDDPWYAQRYVGAGRYWTHATVAATPRSSP"
     gene            43122..43898
                     /locus_tag="PKN91_00955"
     CDS             43122..43898
                     /locus_tag="PKN91_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNS26666.1"
                     /translation="MPGRTLATLTAAVLVTAMTTAYAADAPTVLITGANRGLGLEFAR
                     QYAARGWQVIATARRPAEATALAELAAAHPGVSIETLDVTDGAAIEALAMKLRGQAID
                     VLLHNAGITGARAGHGLGSFDYDLYRRVLETNAIGPMRLTEALLDNVAMSRQKKIVTI
                     SSSEGSITRVDAPRGYWYRSSKAAVNMLMRNLAFEVRKRGITVVLVNPGPVDTDMMKG
                     VRMPLQPVPVAAATVIRIIDGVALEETGRFWDYQGGELPW"
     regulatory      44056..44137
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            44218..45903
                     /locus_tag="PKN91_00960"
     CDS             44218..45903
                     /locus_tag="PKN91_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26667.1"
                     /translation="MQVTAEATAEQGAEPAQYVINLCASTTPMALAQPKSAALKRFTF
                     FVSRRREDGRERFRLHMGYFASTEEADEWLGVVRELYPAAWVGEAPGRKLRAAARAAT
                     PPAAEPVRPPVPPIAAAQAPKPEPAAAREVAPAPPAATSAAPPEMAALSNVREVLALL
                     EDDPPGASDVRKAPAAAADDATDATRSMPVQTLYVPPPPVLPEHIPTVQALTPAPMAA
                     RPESRPTPKPSVPKAAAPKPAAPKPAAQRPAPPKVAAPAPQAPLARPAVPTQKIAPPR
                     VTPPPQPAAASSSLSDTQVLRVLEERRADAGAAPGPQTAEERAAQAAIELLRPDDTRT
                     MRALKDEVQLNVAVQFAVQLDWSVTPIDLDSVPPLAIFSAYTLYTIESNRDGRKWFGL
                     RLGFFSDALSAKQVAYYVRSDFASVAVVPVPAAEKERAIENAHSQPLVPHKRSTRKPV
                     DEFKLFDAEPVEKPPAPSAQAVRAKLEAAARADRNPARARPRPAARKKANSLEDALEQ
                     TLEILGASELEIDNGRGELLNDTGVRHLSVKIDKRTSTFSKLLDRLSDRVGKG"
     gene            complement(45904..46206)
                     /locus_tag="PKN91_00965"
     CDS             complement(45904..46206)
                     /locus_tag="PKN91_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26668.1"
                     /translation="MAHSLVCWKCGASLAALSLPLLRLDECPACAAQLHVCRLCVEYD
                     TRVAKHCREPTAEEVRDKEHANFCDHFKPREGAYVPANTAEIERSRAALEDLFRKG"
     gene            46260..>47231
                     /locus_tag="PKN91_00970"
     CDS             46260..>47231
                     /locus_tag="PKN91_00970"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNS26669.1"
                     /translation="MVKQNATGATGTGSPLLVEFAGRIVMIGCGSIGQVILPLLRKHI
                     GLGPDSLIVLAADDTGRGVAYENGAPFFYSHLKPHRFRRLLARHVRQGDLLLNLSVDV
                     SSLDLVRFAAERGALYVDTSVEPWPGVFDNPMLDVRQRTNFMTRHRALALARELGTDS
                     PTAVVDHGANPGLVSHFVKRALLDLQQALRVDGEPPATRDDWATLARDLGVSLIQVSE
                     RDTQASDRPKRPGEFVNTWSIDGILDESVQPSEISYGTAEKHRPRGSQFHRRDSGTIF
                     LDRPGGATFARSWTPSVGGFQGLMMSHDEAFSIGDFLSLRAGGKFTYR"
BASE COUNT         7338 a        16115 c        16442 g         7326 t
ORIGIN      
        1 gcgactcgag ctgctcgagc gcgagcagca ggaaatcggc gcgcgcgcca ctgcactcgc
       61 ggacgaactc gcgcaacgcg aggctgcgct cgccgctcgc gatgccgcct acgccgagct
      121 gcaacagcga ttcgaatccg gtgatgaaag cgcgggcagg gcgcgcgcgc agctggccgc
      181 cgtgcagggc gagctgacgc agctgcgcga agagctcgcc agcacgcacg agcagctggc
      241 cgcggcgcgc ggggacgcag caggagcacg cgaggcgctg gccgagcgcg agcggcgcga
      301 ggcgagccgc agcgcggaag tcgcggaact gcgcgcggcg ctcgccgcac accgcgagcg
      361 ggccagtacc cgcgagggtc agcgcggggt ctacgagagc ctgctgctcg atgccgaaag
      421 cgatgtcgcg aaccgcgaag cgcgggccgc ggcgctcgag agcgaactcg cgcaggcgcg
      481 ggggcacatc gcctcgctcg agaccgaact ggcgaatgcc ggtacccgca tcaccgccct
      541 catggccgag cgcgacgccg tcaatgcgcg cctgcatgac agcgaagcgg cgatcgccgc
      601 gcgggctgcg gcgcaggcgg cgggcgagcg cgaagccgag gccaggctgg cggaactggc
      661 ggcgcgcgat gcaacgatcg cggcgctcga ggccgagcgt gaccgccagg tcgagacgct
      721 ggcggagcgc gcgcgggaca tcgacgccgg cgtcgtcgag cgcgatgcgc tgcgccaggc
      781 cgtggcgacg ctcgaggagg cgctcgccag gcgcgcgcag cagatcgacg agcaacaggc
      841 cgagaacgag cggctcgcca ccgcggtgat caatctcacc cgggagcttc agggcgcaca
      901 gaccacctac agctcgcagc gcgacagcgt cagtcgccag gacgagcggc tcgagaacct
      961 cgatcggctg ctgcgggccg ccgaggcgcg cgatgccgac cacgaggcca cgatgcgcgc
     1021 cgcgacggcg ctgcacgatg agctcgaggc catgctcgcc ggcgagaagc agcgcaatgc
     1081 cgcgctggcc gaggagctgg ccgcgctgca ggccgcggtc gccgagcgcg acacgcatgt
     1141 cagcagcgcg ggcgaggcgc tgcgctcgca caccgagcgt tcccgggaac tcgagcgccg
     1201 cgagcgcgaa ctcatcgagc aactcgagga gcgcgccggc gcactcgatg cggcgcgtgc
     1261 cgaactgctg gaagccaccg ggcgcgtcgc cgagctgcaa caggacctgc aggccgcgga
     1321 agagcaggtc aaccgggtgg aggccgaact gcgcgccaag caggtgcgcc tcgacgagct
     1381 cggcgccgaa gcgacgcaga cccgcgcgca gcttgcggcg actcggcgcg cgctggagga
     1441 gcgcgatgcg ctgatcggcc gcatcgaggc cgagaccgcg agcagcgccg ccgtgctcgg
     1501 cagcatccgc gagagcatgc agcgcctgga tccgctcgcc gtgacaggca acgaggccac
     1561 cgccgacggc ctggcgcggc tgctgacacg cctcgacgga cctggcgagg tcgtgcacgt
     1621 gctcggtcgc aagacgacga tcgggcgcac gccggacaac gacctgcaga tcaatgccaa
     1681 gttcatcagc cggcaccacg cggtgatcct ggccggaccc acgctgacca tcgtcgagga
     1741 cctcgacagc accaacggcg tctatgtgaa cggccggcgg gtcatgcgcg aggcgctcac
     1801 cgagggcgac ctcgtcatga tcggcaagac gaagttccgc tactccgtgc gcccggtcgg
     1861 cgaccgcaac ccgggttgaa cggcgaacac gggccccgcg atgcaccgaa attcggtaag
     1921 ctgaccgcca cacaattgcg gcggttccct tggcgagtcc ctacatcgag cgcatcctta
     1981 aagcgcgcgt ctacgacgtg gcgatcgagt cgccgctcga gcacgcaccc cgtctgtcgc
     2041 gccgcatcgg caacgaagtc gcgttgaagc gcgaggacct gcagccggtc ttctccttca
     2101 agctgcgcgg cgcctacaac aagatcgcga acctctcgca gtccgcggcc gcgcgcggcg
     2161 tgatctgcgc atcggcgggc aatcacgcgc agggcgtcgc gctcgcggcg cggcggcgcg
     2221 gcgccaacgc cgtcgtcgtg atgccggtga ccacgccgca catcaaggtg caggcggtga
     2281 tcgacctcgg cgcagagacg gtgctgcacg gtgacgatta cgacgcggcg tacgagcacg
     2341 ccctcgagct cgcgcgcgag cgcggcctcg tgttcgtgca cccgttcgac gatcccgacg
     2401 tgatcgccgg ccagggcacg gtggccgtcg aagtcctgcg ccaggcgggc ggggacatcg
     2461 acgcgatctt cgtgccggtc ggtggcggtg ggctgatcgg cggcatcgcc gtgtacgtca
     2521 agtatctcta tccgcgcatc aaggtgatcg gcgtcgagcc ggtcgactcg gcagccatgc
     2581 acgactcgct gcgtgcgggc agccgcgtcg cgctcgagcg cgtcggcatg ttcgccgatg
     2641 gagtcgcggt gcgccgcgtg ggcgaggaga ccttccagct tgcgcgcgat ttcgtcgacg
     2701 agatcgtgct ggtcgacacc gatgaaacct gcgcggcgat ccaggacatc ttcgaggaca
     2761 cgcgctcgat cgccgaaccc gcgggcgcgc tcgcggtcgc cggcatcaag aaattcgccg
     2821 cacgcgccgg gtggcgcggc aagcgcctgg tggcgatcat cagcggcgcc aacatgaact
     2881 tcgaccggct gcgcttcgtc gccgagcgcg ccgacctggg cgcgcagcgc gaggcgctgc
     2941 tcgcggtcga gattccggag cagccgggca gcttcctcgc cttctgccag gcgctcgggc
     3001 cgcgcgccat caccgagttc aactaccgtt acgagagcgc gcacgccgcg cagatcttcg
     3061 tgggcttcgc gctttcgcag ggtcgcgagg agaaggacgc ggtggtggcc gcgctgcgcg
     3121 ccgcgggcta cctcgtgcag gacatgacgg acaacgaagt cgccaagctg cacgcgcgct
     3181 acatggtcgg cggccatgca cgcggcatcg aacacgagtt ggtgttccgc ttcgaatttc
     3241 ccgagcgccc gggtgcgctg ctgcgcttcc tgcaggcgat cggcacgcgc tggaacatca
     3301 gcctgttcca ttaccgcaac cacggctcgg actacggccg cgtgctcgcc ggcatccagg
     3361 tcccgcccgc cgagcgcgag gattttctgc tgcatctggc cgagctgcat tacgagtatg
     3421 tcgacgagag cgacaacccc gtctaccgga tgttcctcgg cgcgtgaacg atatcgacgc
     3481 tgcctatgtg ccgcgccgcg tgccggtgca cgaggatctc gtgctgcgtg gtctgcgtca
     3541 tcgcataacc cgctggggtc cgccctcgcg ctcgccgatc gtgctgctgc atggtttcct
     3601 cgacgcggcc gacagctggc agttcctcgt cgatgagctg ccggaggact ggtcgttcgt
     3661 cggcctcgac tggcgcggct tcggggggtc ggcctggcag caggacggct actggttccc
     3721 ggactacctc gccgacctcg aggccttgct cgcggtcgtg gccgatgcgg cgccggtacg
     3781 cgtgatcggg cacagcatgg gcggcaatgt cgcggcgctg tacgacggca tccgccccgg
     3841 ccggctccgc tggatcgtca atctcgaggg cttcggcctg gcggacacga aaccggacga
     3901 tgcgccggcg cgctatgcac gctggctcga cgagctggcg aagcccccgc ggcagtcgac
     3961 ctatgcctcg ccggaggatc tcgcgaagtt cctgcgcacg cgcaacgcgc ggctcacccg
     4021 ggcgcgtgcg gcattcctcg cccgcgcctg ggtacgcgag gatggccggc gctggcggct
     4081 cgccgccgac ccgcggcatc ggctggtcaa tcccgtgctc taccggcgcg aggaggcgct
     4141 cgcctgctgg cggcgcggca ccgcacccat gctgctgctg ctcggggcgc tgaccgagta
     4201 ccgcgcgcgg ctcggcgagc acggcagcga cgggcactta cgcgcgtcgt tcccgaatgc
     4261 catgatcgac acgctggccg gcgtcggcca tatgatgcac cacgaggatc cggccgccgt
     4321 cgcgcgcgcc attgaacgat tcgcccggat tcaggtccca gacgtttcat gaactggaaa
     4381 tggcccgatc tgcatcgcga ccatccgctg cacctgccct acaggtggat gaagcgcatc
     4441 gtcatcacct tgacgggctg cacggtgctg gcggtgggtc tcgcgatgac ggtgctgccg
     4501 gggccggcct tcatcgtcat tcccgcgggc ctcgcgatcc tcggcctcga gttcgcctgg
     4561 gcgcgccata cgctgcaggc catcaggaag cgcggcgccc tgatcgtcga ggcggccagg
     4621 tcgaagctgt cctagagcgc cgccgcgatc agcgcgcgga attcctcatg gctcttcgcg
     4681 ccctggatgc gccgcacgac ccggccgtcg cgcccgagca ggtaagtcgt cggcagcact
     4741 tcgttccacg ccggatcgac cacgctcgcg atcgcgtcga gctcgcgctc gctgcgcagg
     4801 tagctgcgaa agtccgggaa gtatttcgcg tggaagggcg cgacccgcga ctcgcgctca
     4861 tcgggctcgt ccatcgccac gccgatcagc acgacctgcg gcgcaagctc gcgctcgatg
     4921 cgcaccaggt ccgggatttc acgcaggcag ggcacgcacc aggtcgccca gaggttcagc
     4981 accacgacct tgccgcgctc ggcgtcgagc gccgagcgca gcccgccggc cgaaacgagc
     5041 gcgatgccgc gatcgtcggc gtgcgtcatc tgctgcgcaa tgagtgcgcc acaggcggcg
     5101 aggaagtcac gccgccgcat cactcggccg gaccgattat cgcggtgtag atcagatcgc
     5161 cctgctgcca cgcgatctgc acgcggccgt cgtggcgcga tgcgagcgcc ggatgctgcg
     5221 ctgtttcatc gccgctcgtg agcgtactgg gcgcatcgag cggctgaccc gcggcatcga
     5281 gcgtgcgata gaagatacgg cgcgggccgt cggccttcgc gtgccatgcg accacggcac
     5341 gatcgccgcc cgcgagcgcg atcgccggcg catcggagac ctgtgcgccc gggtgcgccg
     5401 gatacgcggc ctcgaagctc gcgccgccgt tgcgggatac cgcgagcagc acgccggggg
     5461 ggtcttccgc accattgtgc acggccgtgt agacagtgtc gccgcgcacg gcgaggacgg
     5521 tcggcttcag cgggcaacca tcgatcttcc acggcgcgcc gcccgtcgat acgcgcgtgc
     5581 cgaagctgcc gtcgacgcca tcggcgcgcg ccaccgtgct ggcacgcacg ccgtcgggcg
     5641 tcacgagtcg cgagccgagc caggggcgcg agtcggcatc gaaggcgagc gacagctggc
     5701 agcacgggca gaccccacca gccgcctcga cgcggtcctg cgtgaatgtg cggccgtcgt
     5761 cgtgcgacac cacgccgtag atgctggcgt tgtcggcgcc gcttttcatg tcgcgcgtgt
     5821 cgatccacac gaggtgcacg tcgccgttcg gcgcgacgcc catcgtgccg aatgccgcca
     5881 cgctcgcgaa gcccccgtgg atgacctgcg cgaggtcggc atcggtgacg gtgctgagcc
     5941 tgcgcggcac ctcgaaactc gcaccgccgt cggtcgagcg cgtgtagcgc gcggtgagca
     6001 ccggcttgcc gagttgcggc tgcgtctcgt tggccggata ggcgatgtgc agcgtgccgc
     6061 ccggtccata cgcgacgcgt ggccggctca ccgcgaagcc ccacacttcg ttgtcttcgc
     6121 gattgacgcg cagcggtgcc ccgaagctcg cgccgccgtc cttcgagacg gcgacataga
     6181 gattcatcga cgacagatgg gtgtggccgt gcttcgcgcg atccgcctcg gcggccttcg
     6241 cggccgcgga ttcgggatta cccttcgcga gccacagcac cgcgatctcc ccgccgggcc
     6301 ccaccgccac gtcgggagcg agtgccagct gctcatccgt gctcaaggcg agcggagtcg
     6361 tcgcaggtgg cgctgcagca gcgggcgcca ccagcgccag cgcggccagc actgcgaggg
     6421 cgatcgtggt ctggttcggc atgccaggtt cccggtggcc ggtgatccgc acattgtagg
     6481 cctgaccatg cgagtcctgc gacaatggcc gcaatggaag acatcgcctc cgctccgtgg
     6541 ttgcgtcgcc tgatcgtggc ggccctgctc gcaggcctca tcgtgctggc gttcgaggtg
     6601 ctgaagccat tcatcgtgcc ggtcatctgg gcggtgatcc tggcgcacgt cacctggccg
     6661 ctctaccggc gcgtgctgca cgcgatgaaa cagcgccgcg cgccggcggc gctgctgatg
     6721 acggtcgcgg tcacgctggc gctggtcgtg ccggcgatct ggctcgcgga actgatccgc
     6781 accgaactgg tggcggccta tggggcgttc gccgagcgcc tcgcccaggg ggtgaccttg
     6841 ccgcggttcc tgctcgatct gccctgggcc gggccctggc tcaccgagtt gctcgcgcgc
     6901 attgccgacg atcccggagc cttgaacgtg gaagtgctca cgtggctcga gcgctcggcg
     6961 ggggagatcg gcggcgtgat cggcggcgtc ggccgcaaca tcgtcaagct cgccattgca
     7021 atgctgagcc tgttcttcat gtaccgcgat ggcgagtctt tcgcggcgca ggtcacgcgc
     7081 gtgctggagc agtcgctcgg ccagcgcgtg cacggctaca tgctcgcgat cagccagacc
     7141 gtgcgcgcgg tggtgtacgg gctgctggcc gcagcgcttg cacagggcac gctcgccggc
     7201 ctcggctact gggtcgcggg cctgaacgcg ccgatcgcgc tcggcgccct gacgatgatc
     7261 gcggcgctga ttccgttctt gaccccggtc gtgtgggtgg gcgccagcgt ctggctgctg
     7321 atcaccaacc acgccgtcgc cggcctcggc ctcttcctct ggggtctgct cgcggtcagc
     7381 tggatcgaca acttcgtgcg tccgctcgtc atcagcaacg ccacccgcat accgttcctg
     7441 ctggtgatgt tcggtgtgct gggcggcgtg ctggccttcg gcctcgtcgg cctgttcgtc
     7501 ggcccggtga ttctcgcggt gctggtcgcc gtgtggcgcg aatggctcac cgacaagtga
     7561 gggatgccgg gtctaggcat ccaggaaccg cagcagcgtc ggaccccact ccgccggatc
     7621 gcgcgcgagc gcgcagaagg ggagctgcgc atgggtctgc gcggcggcga gcgcatgttc
     7681 gcgcatggga tcccagtcgg cggcggcgag cagtgtcgcg accttcacgt cccgcagccg
     7741 cgccttcacc ggatagctga agtgcgcacg ataggccgcg cgccacgccg gcgcgcgcat
     7801 cacctcgagc acgcgtcgct gcaccatttg cgtatcgacg ttgggctcgc cgcggatgat
     7861 gccttcgcgc gtggtgttgt accacggcca gaacaggccc tggtcgcgca ggaagtgcca
     7921 ggccctgagc aggtggccgc catgcgcgtc gacctgcaga tcgggcgcgt aacgttgtcg
     7981 cagctcggcc tgcaacgcgc ggtcgaacca caggggaccg accattacga ggcggcgcac
     8041 gcgtcgcggt gcaaggtgcg cgagttccag gccgacgagg cctccgcccc agacacccac
     8101 gatgtcgcat tcgcggatgc cgagcgcgtc gagcgcgccg cgcagcgcgc gcgcgtaggc
     8161 ggcgacggtg acggtaccgc ccggcagggt ggagtcggat tcgccatgcc ccggcaggtc
     8221 gatcgcgagt accggccggc ggccgatgaa cgacgcggcg agagccgcca ccgtgtcgga
     8281 ggatccggcg gcgtcgtgca acacgacgac ggtgcgcccg ggcgcatcgg tgttgcgacg
     8341 gatgcgcagc tgcccgccgc gcacatcgag gctctgctgc cagagccgcc cggcgatcgg
     8401 ccgggtcgga gcgagcttcg gcgcggcgct cggccgcgcc ttgcgcaggt gcgcgaggca
     8461 cagctcgagc gtttcgagcg ctgtcgcgca ttcccgcacg gtcacgttgc gcggcgggtc
     8521 ggtcgcccgc acgagatagc gtgacgtcgg gtcggtcgat gccgcggtga tcagcgccgg
     8581 caccctgatc tgctgcagca cctcggcgct cggcatcgcg aaggccgcgc gataggcgac
     8641 gcgataattg tcaccggcgc gcagcatctc gacgaggccc gcctgcaggc tggcgggcga
     8701 cggcaggttg acgtgcatcc ggtcggcggc gcccttggcg taccacggga agaaaatcac
     8761 ctgctcgcgc atgcggctcc acagccacgc gaggtgcccg ccgtcccaag tcggcctgaa
     8821 cggtggcagg tagttcgcga gcaggtcgtc acgctcttcg cgggtcggga tcacgtagcc
     8881 gttcgcggcg acggcgtgca cgcgctccgg gtacttcgcg gcgacctgtg ccgccatcat
     8941 cgcgccggtg tgatagccgt agaaggccgc cttgcgaacc ttgatcgcgt cgaggaactc
     9001 gatcaccgca tcggcgaaat cgcccatttc ggcgctctcg acgccgagcg tatcggactg
     9061 cccgtagccc ggcgtgtcgg gcgcgatgca ggtgaagtgc cggtgccagc gctgcatcgt
     9121 cggcaccagg tcgcgcgagg acagcggcga ctggtgcagc aggatcagca gggggccctt
     9181 gcccgtgcgt cggtagtgca cctggcgcct gccccagcgg ccctgttcga tcgtggcgaa
     9241 atgacgagtg atgcgtacgc tggccatggc tgctcccggt tggatcgtgc ggattattgg
     9301 ccaaaggcga ctcggtgcgg cggacgaagt atgctcacca ccaatccatg gcctcgtgca
     9361 acgcaatgcc gccgcgtaca tgagcgtgcg ccgtcctgca gccctgcgcg ccctgcgcca
     9421 tcgcgatttc gcgcgctttg ccgccgcgcg cttttgcgcg acgctcggat ggcagatgct
     9481 gagcgtcgcc gtgggctggc aggtctacga attcaccggc gatccgctcg cgctcggcct
     9541 ggttggactg gcccagtttc tgccgttcgt ggccctcgtg ctgccggcgg ggcagatcgc
     9601 cgatcgcgcc gaccggcggc tgatactcgt gctctcctac gcggtcgagg cgctgtgcgc
     9661 actcgccctg ttcgcgctcg cgctactcga cctcaggatc acctggccga tcttcgtggc
     9721 gatgctcctc ttcggcgccg ctcgcgcgtt ctggatgccg gccggccagg cgatgacgcc
     9781 gaacctcgtg ccggcggagg acttcagcgc ggccgtggcc gtcaattccg cgttgttcca
     9841 ggtgtcggtg atcgcggggc cggcgatcgg cgggctgctg ttcctcgccg gcgcggagat
     9901 cgtgtatggc agcgtgctgc tgctgctcgt actggtcgtc gtactgatgc tgcgcatacg
     9961 gccggtgagg gtcgcgggtt cgggcgacgg ctatcacctg cgcgacgcgc tcgaaggcct
    10021 gcgcttcgtg atacgcaaca agccgctgct cggggcgatc tcgctcgacc tgttcgcggt
    10081 gctgctcggc ggcgccacgg cgctgctgcc ggtctacgcg agcgatgtgc tgcacatcgg
    10141 cccggcgggc ctcggcatcc tgcgcacggc gccaggggtc ggcgcggccg tggttgcggc
    10201 gtggctcgcg gtggcgccgc tctcgcgcca tgtcgggcgc tggatgttct tcggcacggg
    10261 actgttcgcc ttcgcgaccg tcgtgttcgg cctctccacg tcgttcgcgc tgtcgctcgt
    10321 agcgctggtc ctgctcggtg cgggcgacat gctgagcgtg ttcatccgcc acattctcgt
    10381 gcagctcgag actcccgaca gcatccgcgg gcgcgtcagc gccgtgaacg cgatgttcat
    10441 cggcacctcg aacgagctcg gtgaattccg cgcgggcgtc gccgcgcgtt acctcggcgc
    10501 agtgccggct gtggtctggg gtggcgtcgc ctgcttcggc gtcgtgctcg cctacaccgc
    10561 gctgttaccg acgctgcgca ggctcgaccg ctttccgtgg ccgaacaggg gctagacgag
    10621 gcatgcgcgc atggggactc aggaaggcgc gcacagcgca taccctgatg tcggcagcgc
    10681 atacgcttga ccggatgaca ggcggcacgc atggttcgcc aaatgaggtt gccgcattcg
    10741 ccgacgcccc gtgtaccgcg cgatcagcgc cgccgcgtgc tgctcggcat cgcgacgctg
    10801 ccgttggcgc cgctggcggt ggcgcgcgcc cggtcatccg gtttttccct cgcactgatc
    10861 gggcagtgcc tcatccgtca cgatctgtgc gcgcagccct ggcccggtct cgcgccgctc
    10921 gcacggcgcc tgcgtgcgaa cgacgcgtgc ttctccgatc tcgaagtcgc catccgcgga
    10981 ccgcgcgaag gggcacccac gcgggattca gggaccctcc atacggccga tcccgtggtg
    11041 ctcgattgcc tgcgcgcctt tggcgtgagc tttctcgcga cctcgaacaa ccacgcgttc
    11101 gacatcggaa cgggcggcat tctggacacc atcgatgcgc tgagggcgcg ccaattcgca
    11161 ttcgccggca gcggccgcac tctcgctgaa gccgccgcac cggcatatca actgaccgcc
    11221 gcgggtcgcc tcgcggtggt cgccgcggcc gccgggaaga tccgcgacgg cggcgcggcc
    11281 acggacgcgc ggcccggcgt ccatgagctg cgcagtgccg agggcggcct cgatgccggc
    11341 gacgtcgagc gcatgctcga atcgctgcga ctcgccgcgc gcgaagccga cgtcgtgatc
    11401 gcctacctgc acaatcacct gtgggcggaa gagatctcgg acactcccga ttggcagcgc
    11461 agcttcgcgc gccgttgtgt cgatgccggg gcgagcgtgt tcgtcgcaca cggcactccg
    11521 ctgctgcaag gcctcgaact ctatcgcggc gcaccgctgt tccacggcct cggcagcttc
    11581 attttccaga cgcgtaagcc ggtcggcgct tacggcgagc cgaactggga gagcctcgtg
    11641 gcggaatgcc gctttgaaag gggtcggtta atagaagcgc agctcacgcc cgttgctctc
    11701 gcaccagtgg gcatcggcgg cccggacgat tacgacacgc ggggccgccc ttcgcctgcc
    11761 gacgcctcac aggcgcgcga gacactcggg cgtgttgcgg gcctttcgca gcgactcggc
    11821 taccgcctgc gccatgatgg cgcgcgcgcg acgctccgcg ccggataggg tgcgcgcaag
    11881 tcgaccgcac cgttcatgcg ccgcgcacgg gcagcacgtg aagcgtgacg tgcgatggcc
    11941 cgcgggggcc caggtgtaag cagttgtcgg ccaccacggg ctcgctcggc acaccggcgg
    12001 gcgcgcccgt gttcggattg atatcgaagt gcgggaaatt gctgctcgac acgtcgatgc
    12061 ggatgcgatg gccggcgacg aagcggttgg cggttggaaa caactccaca tcgatcgcgt
    12121 agacgcgtcc gggttccgcc ggcaacgcgc gatcatagcc ctcgcgaaag cgcagccgcc
    12181 ggatgccgtc ggtcagattc atcgcgtaac catctggcca gtccggcgag ggtggatgca
    12241 cgtcgagcag cttcaccgtg aagtcggtgt cgagcgcgct cgtcgacacc cagaggcggg
    12301 cccggaccgg gccggcgacg atgacatcgt ccacgagtgg ctcagtttcg aacacctgca
    12361 catccgcgcg cgccgcgagc gcgcgccccg gatgctttgc gccgtaaatg cctggcgttt
    12421 cgcgctggtc gaatgcaccg ccctccatga gcggggcgcc cgaggcgatc gcgccgccga
    12481 tcgtcggcac cgggtcgcgc ggatcgaagc gccaggcgat cacctcgtca tggcagggct
    12541 gctgcgtggc gagtacgccg cccgcatgca ggtaccagtg agcgggacgg gcctccggtg
    12601 gtggccagtc ggccgtacgc agccaggcgc ctccgtgcag caatcggccc tgtggcgtgc
    12661 gcgtgcccgc gccgccgccc atgacgaaca cggtgacagg cgctgccagc cagtcgggcg
    12721 cgtcgcgccc gagcaggtga cggtcgaaga aatcgcggcg cagcgtcatg tagtcaggag
    12781 cgacgttgcc gtcaagtgtc gccgacggcc cgaagtccac cgcgccggcg tgcgacaccg
    12841 aacgctggcc gtgcgtccat gggcccatca cgagccgcac cggcccgcgc ttcagcttcg
    12901 acagcgcgac gaagttctcg gtggccgtga gtgcatacgg gtcataccag cttgacatgt
    12961 gcaccatcgg cacgtcggcg aaacggtcgt agctgccgcg cgcgtacagg cccggccgct
    13021 cccagaaggc gccgaaacat tcctgcgacc actgctcgat gacatacgct tcatattccg
    13081 gcgcggcact cactgccgaa tgatctcgtg tccacggcat cacgcgtgcc caggtccgta
    13141 tgtcggcagc ctcgagcgca gcccggcgca cggggtcagc cgcggtggcg ggcgagaggc
    13201 gtgcgtgctt gagcgcccag gtcagctgct tcagttcgaa cgcgccgccc tggcggatgc
    13261 cgctgtgaaa cgcgctcgag aagccccccg aatcgatgaa catcgctccg agtcccgcgg
    13321 gtgccgccgc cgcgagcgcc gtctgcacgt gcgcaccgta agagaaaccg agcgtcccga
    13381 tgcggcggtc gcaccagggc tgggccgcaa tccacgcgag cgtgtcgatg ccgtccggtt
    13441 gttcgttcag gtacttcgtg aactcgcctt cggaatcgaa acggccgcga cagtcctgca
    13501 acacgcaggc gtagcccgca cgcgcgaact gcatggcgat ctcgggcttc gacttcggca
    13561 cgggatctgc aaggctccgg tcggcatgat tcgtgccgcg cctgccgtag ggcgtgcgct
    13621 cgagcagtgc gggccagggg cccgtgccct gtggcaggta gatatccgtc gcgaggtgca
    13681 cgccatcgcg catcgcgacg cgctcggtgc gccgtcggat tccaggttcc gcgctcacgg
    13741 taccgccggc agatgaatgg caggcgcacc cggcccgacc ggcaggccga gccctgccca
    13801 cagcgccacg agcagcagcc ccgcgatgcc gaatgccgca ctgaagggca gcatcatcga
    13861 gatcagcgac ccgacgccga aaccgcggac ccagcgctgg caatacacga ggaccagcac
    13921 gaagttggcg gcgactggcg tcacgatgtt gaagatcgag tcgccgacac ggtaggcggc
    13981 ggtagtcatc tcgggcgaga tgccgagcaa catcagcatg ggcacgacga ccggagccat
    14041 cgcgctccac tttgccgaag ccgatccgat cagcagatcg aacaccgagg acacgagcag
    14101 cagcggcacg agcatcgcca cgggcgagag cgccagtccc cgcagccagt tcgcgccatg
    14161 gatcgcgagg atgggtccga gattcgacca tgcgaacatc gcgatgaagt gcgacgcggc
    14221 gaacgcgatg acgagatagg gtgcgatgtc gcgcagtccg tccaccatca tccgtacgac
    14281 atcgcgatgc cccgtgatgc tgcccacggc gcggccatag acccagccgc aggcgaggaa
    14341 cagcagcagg aaccccgcga tgagtgcgct gtagaagggc gccttgcgtg catagccttc
    14401 ggagtccgga tccagcaatg gcgtgtaccc gggccagagc acgagcgcgg cgaagacggc
    14461 aatgaccaac agagccgcga cacctgcgcg gcgcaggccg cgtcgctgcg cctcgctcgg
    14521 tacggtcggc acggaatcga ccccgatcac ctcgagcgct tcaccatcga cgacgaccgc
    14581 cgcgagtcgg ggagcgaccc agcgctccgc gacgggccag gcgacgcaga acagcaagcc
    14641 gccgagcgcc gctgtgaacc accagttgcc gagcggattc aggccgcgtg tcggttcgag
    14701 cagttgcgcg gcactctgcg tgatgccgag catcagcgca tcgaactgtc ctggcagcag
    14761 gttgcctgcg aacgcgcccg agatgccggc atacgccacc gccatgccga gaagcgggtg
    14821 gcggcccgct tcgcgataga cgatggccgc aagcggaatc aagacgacat acgcggcatc
    14881 cgaggcgtga tgcgacaaca gcgcgatcag gaacgtggct ggcgtgagcc acgcgcgcgg
    14941 tgccgcgctc acggctcgcc ccacgagcgt tgccagcagg ccagaccttt cggcaaccgc
    15001 cgcgccgagc atcacgacga cgacgagccc gagcggcgga aagcgcacga acgtctgcgg
    15061 catctcgacc agcaggcgcc gcacgttcgc ttcggagagc aggctctcgg ccgcgatgcg
    15121 ttcgccggtg agcggatgca ccgccgcgac accgagcgac gcggctatcg cggatgcgac
    15181 gatcagcgcc gcgatcagcc acaggaagat gacgaccggg tcaggcagcg cgttgccgag
    15241 ccgctcgatg cgatccagca ccactctcat ggcacgcgaa cttccatcag gctgcccggc
    15301 gggttgcgca tagcgtaccg gctcaatcac gggacacaat atgctggcgc gcgctcatca
    15361 ggccatgcgc tcccggcatg attcggcggc gatcgcgcgg cgccgtcagc ttgcatcggc
    15421 cagactccgc ggctaccttg caggcgtgaa gatcagcctg cgcggactgg agagtttccg
    15481 cgaaatcatg cggaagggtt ccgcgacagc ggccgcgcgc gagctgagtc tcagccaacc
    15541 ggctgtaagc cgcctgatcg ggcaactcga gcgcgaactc ggcttcaacg tatttgatcg
    15601 catcaagggt cgactggtgc ccacgcagca gggcttgctg ctctacgaag aggtggagtc
    15661 cgcattcctg ggtcttgagc gcgtgagcac ggtcgcgcgt gacgtgcatg acctgaacgc
    15721 cggtcaactg cgcattgttg cgccgccgag cctcgccgag ggacccctgg tgggcatcat
    15781 cggcactttc ataaagaaat atccgaatgt gcgggtggta ctcgactcga actcccgtcc
    15841 caccgccatg aacatgatcg ctacgcgtgc cgccgactgc ggtttcggca agctgcccct
    15901 ggagcatccc ggcatccaca agcgcccact ggtggtgaac gaaacagcct gcgtggtccc
    15961 gcgcgggcat cgactggcgc gcaaaccgac ggtgagtgcg accgatcttg ccagtgaaga
    16021 gctgatcatg atcggtcgcg ggagcgatac gcgattgcgg atcgaggcgg cacttcgtgc
    16081 cgccggcgtc gttcctcgca tacgcttgga aacccacaac gtcggggctg cctgcgcgtt
    16141 cgtggcggcc ggtgtcggca taactctcgt caacgacatg ctcgcacgcg cctattgtaa
    16201 gcgcgagatc gagctgcgcc cgttccggcc acgaatcatc catgaataca tattcatgac
    16261 atcgtccggc gcgtccgcat cgccgctgac gcttgcgttc tatgccgcct gccgggatgc
    16321 actccatcgc taccacgccg cgtgactcgc aggcaggggc cggcgccggg caacgttcag
    16381 cattttcacc ggcgccctga tccgccccca ggagcgtcac atcctggtca tcagccgtat
    16441 gaaatagtag cggccacgaa cgtcataggt gtagatgtcg aagttcatgg ggttgttggc
    16501 ccgcgagatc ggcggcattt catcgagcac gttgtcgatg ccgagggtga tgttggagtt
    16561 gaatcgctcg aagtgatgac ccaactgcag gtcgtggtag acgacgtcgt ccgtgcggcc
    16621 tccgttgagg gtgttgcccg ccacgtccgt gctgctgccg atatagcgcg ccttgtagcc
    16681 gccgctccat gggccctgct cccagcgcag ccccgcgctg cccttccact tcggataagt
    16741 ggatcgcggc gtgtcgccgg tgccggccag ctcggtccgc tgaaccgtgc cgtcggtctg
    16801 cggcacaaag ttcgtgaaat ggaacaggcg cgacgcatcg agcactgcat tcagccggcc
    16861 gatcggcgta tcgaagccgt agcgccaggt gacgtccagg ccctcgacct cgatgctgct
    16921 gaaattcagc acgacctggc gcagatcacg tacctctccg gtgctgaggt ccctgtccac
    16981 caggttgcag tacgcgccag ctctctgggc gcacagggtg agcagctgct gtgcggactg
    17041 gctcgcgatc gcctcatcga tctcgatgcg gtaccagtcg gccgtgagtg acatgcccgc
    17101 ggcccagccc ggcgtgtagg cgaagcccac gctgtaggta tcagcggtct ccggtttcag
    17161 gtccgggttg ccgccgatcg ttccgcgcag ggtgcctgat ccgaactggt tctgattgta
    17221 ggtcgcaggc acgccagcac accctggcaa cccggcgccg ccaccgttgc atggatcgac
    17281 tgcctgaaag gtcgtctgcc gctgcccttg gtagagttcc aggatagacg gtgcgcgaaa
    17341 gccctcggag aaggtgccgc gaatcagcag gtcatccacc gggcgccatg ccagcccgag
    17401 cttgctcgtc gtggcattgc cgaagctcga atagtcggag tgacgaactg ctgcgctcag
    17461 atcgagcgct cgcgcaccgg gcaggtccgc gagcagtggc accgcgagtt cggcgtacgc
    17521 ctcccacaga tcgtacttgc cacgtgtcgg ctcgcgggtg gaggaagtcg tgcgtgtctc
    17581 gttcgtgatg aactccggcg cggagttgac atacgcatcg ggatcgtcgt acgcggattc
    17641 ttcccgccat tccacgcccg ctgccactcc aacgcggcca gccggcaggt cgaccaggct
    17701 gccggagatg ttgaaagcca cgtcggtctg ttcggtgccg ttgtcgttgc gcgcggtata
    17761 gcggatgtag tcggccattt gcggcgtcat gacgccgaag atgttcaacg gtacgcagtt
    17821 gttggccgca cagcgatcgt tcggtccgag cgcgagcgcg atcttgtcct ggtctgcctg
    17881 gttgtaggca ttgaatgtcg cgtcgttctt ggcgtgcgat gcgaacagat cccagttcca
    17941 aagccccgcg agctcgaatt cgccgtccag gccgaaggcg atccgctggg tttccacatc
    18001 ctgcacgttg ttgcgattgc cgacctcgac cggcatgcgc tgcaatcgca gtgaattggt
    18061 ggtgaatgcc gtctgccagg gattatacgg atgatcggcg ggaatggtga tcgagtacga
    18121 tccccgcagg tcgagcagga gcggtgagaa cagctgattg gacttgcgct cgttgtagag
    18181 cagttcgata tggccactca gcgaatcgct gaactccacg ccgatccggc cgaagacgcc
    18241 gaagcgctcg ctggggccgg tcgcgtacac gccctgggcc tgagtgttgt agtaatcatc
    18301 cggaagggtg gcgacgcgga aatccgccgg atcgtcgcca ttcgtatcgg gaatccgggt
    18361 gaccgccctg gtcgctaccc ccgggaggag atagagaccg cgcggactgc tcggcgggtt
    18421 cgtcggcgca accgccgcga ccgcggtaag ggaccgatcc attgtcagaa cggggtcggt
    18481 atttacgtag ctcgtcgaca gcagtgcgga tacgcgacca aagctgtgac ccatgctcag
    18541 gtccgcgctg aggctctcgc cgtcactgtg cgaggtctgg ccgtagttga ggcgagcctc
    18601 gatgccctcg atgctgcggc gtgtgcggat gttcaccacg cctgcgatcg cgtcggcgcc
    18661 gtagatcgcg gatgcgccat ccttcagcac ctcgatgctg tcgaccagtc ccatcggaat
    18721 ggtgttgaga tcgacgaagt cgcggaaggt gcgcccacct gccgcattca cccagcggtg
    18781 tccatcgacg agcaccagca cgcggttgcc gctgccctcg gcgctgccga gataacgcag
    18841 gttgatcgag ctgacaccga atgaggtgcc ttgcgtgccg ttcgaattga ggctgactcc
    18901 cgctcccggc agctcctgca acagttcacc caccgacagc gctgcggcga aagtgatgtc
    18961 ttcggcagtg agcgtcgtca gcgggccgac cgattcggcg tccgcgcgtg cgatgcgtga
    19021 acccgtgacc gtgacttcct cgatcgcatt ggacgcgaca tcctgggcgc aaaccgcgag
    19081 ggggagcaac gctgcaatgc ccgcggagcc gaggcgccgc cacgcaaggt gtgcagtgga
    19141 cgatctccgg gaaacgaacc gatcgggtct cgagttgtga ttcgcatgat ctgtcgacat
    19201 gagtcggatg ctcccccggt ttctggtcgg aattctggat tcagcgcccg cgcccgcggc
    19261 tcgtcggagt ttcgatttcc gcgcctcacc gtgcacgtga cgcactcgtc gcggcagatg
    19321 tgaggtcgtg cagcactcga tgcgttccgg ggcgcgaccg cgagcgaccg tagagcaaag
    19381 ccagcagtgc aacaagatta ttgcgtgttg catcgaccta taagcatgct gtatagcaaa
    19441 gacgtttccg ggaactgcca gtactctggc cgcattcctg caatccggaa atgaatcggg
    19501 gacgcatgtg ctcaggcgcg gccgactgta cgtagccact ctcgctgcaa tcattgtgtc
    19561 tgctgcctac gcgggcgagg tgccagcgcc gacggcgctc attcatgcgg gccatcttct
    19621 cgaccggcca ggctcgcagc cgcgctcgag tgcgaccgtg ttggttcgtg atggcaggat
    19681 tgcggaggtc cgtgatggct ttgtcgatcc gcaggacttc ccgggcgcgc aggtcatcga
    19741 tatgcgtgcc tacttcgttc tgcccggcct gatcgattcg catgttcact tcaacggcac
    19801 cgagtccggc aaaggggcat tgctggaacg cctgagcaag gacaccgcgt tcttcgctta
    19861 ccgggcagca acactggccc gcctcacgct cgatgcgggc ttcacgacgg tgcgggtgct
    19921 gggcaacccc gccggcgtaa cgctctcgct ccgcgatgcc atcgcagccg gcgccctgcc
    19981 ggggccccgc atcctcgacg ccggcaacag catcgccgcg acgggcggct cgggtgatgt
    20041 gacgcttggc gtgcactcgg cgctggccgg gcatatggat caggacaacc tgtgcgacgg
    20101 tacggaatcg tgccggcgtg cggtgcgcct gcaagtccgt cgcggcgtcg atctcatcaa
    20161 actcaagatc accggtggag tgaacagccg ggtcgcgacc gggctggggc gccagatgtt
    20221 catcgacgag gcgcgggcgg tcgtcgagac agcgcacctg tacggcaaga aggttgccgt
    20281 gcacgcgcat ggcgctgacg gcatcaatct cgcgctcgag tgcggcgccg actccatcga
    20341 gcacggaacg ttgctcgatg accagggcat cgagttgatg ctgcgctcgg gtgccttcct
    20401 cgttcccacc ctttcgacag ccaccaccta cgtggagcgc ctcgcgaaca accccgggac
    20461 ctatacgcaa gaggtacgcg agaagatgga gtggcgcgca tccgttaccg gacaggccct
    20521 gcggcaggcg tacgcccgcg gcgtgcgcat cgcctttggc accgacgcag gcgtctcgcc
    20581 gcacggacgc aatgcggagg aattcgtgct catggtgaag tacggcatga cgcctggcga
    20641 cgccatcaag gccgctacgc tcaatgccgc agagctcctc ggtatcgccg atgaggtcgg
    20701 ctcgctcgag ccgggcaaac gcgccgacct cgtcgcggtg cgcggcaacc cgctggaaga
    20761 cattgctgtt ctgcagcatg tgcccttcgt gatgaagggc ggcctgatct ggaagcagta
    20821 gcaatcggcg gttgccgggg gcgagccgcc tcgcgtgtgg aggtgctgga ctcggcaact
    20881 gtatatttgt acagttcgcg gatggcaggc tggctcaaag gacgtggtgc ggtatcgaac
    20941 ccggctccac ggttcgagcg actccatgaa ctcggggtcg acgacggctg gtacactgag
    21001 cccgcgcccg acagcatcgc gacgctcgtg cagcccgaac cggcgcgcag cgtcatcagc
    21061 cgcaacgatt cgcccgacat cggcttctcg cagtcgatca acccgtaccg cggctgcgag
    21121 catggctgcg tgtactgcct ggccggcgac accgcgatta ccatggcaga cggaagtgcc
    21181 aagccgctgg cgtcgctcga gcggggcgac gaaatcattg gaacggtacg acgcgggcac
    21241 taccgccggt acgtggcgac ccgggtgctc gcccattggc gcacggcgaa gccagcctgg
    21301 cgcgtcaccc tagctgacgg aacacgggtg atcgcaagtg gcgatcatcg ctttctgacc
    21361 gagcgcggct ggaagttcgt ggccgcctcg ggctcgtcgc acgctcgact gacattgaac
    21421 aactcactgc tgggttttgg tcatgtggcc caagtcgaca gatgtgagac ggagagcgcc
    21481 aagcgaggct acctcactgg cgtcatccgg ggtgacgggc acctgaaggt ctattcgtac
    21541 cgccgggaag ggcgagcttc aggagatcag caccgcttca ggctcgcaat gatcgattcg
    21601 gaacctctgg ccagagcggc gtcgtggctc ggcgaatttg gtgcgccgac ggcccgctac
    21661 gaattcggca ctgcgacagc gggacggcag gccatgggcg cgatccgttg cagctcgcgc
    21721 gcctcggttg ccttgattac gcatctcgtc aggtggccgg aaactgcgga ccgtgactgg
    21781 cagcgaggct ttgtcggtgg aatattcgac gccgagggct cgttctccga tggaatcatc
    21841 cgtatcggca ataccgatcc ccggatcatc tcggagttga tcacggcgct ctgcgccttc
    21901 ggcttcgaca ccgtactaga atcgatcccg cgcagagatc gcaagcccat gcagtacgtt
    21961 cgcctacgtg gaggactcgc acagaatttg cgctttttca ggctttttga cccctgcatc
    22021 gcccggaagc gcaatatcgc cggtgccgcc gtgaagtcgc gcgccgatct gcgggtcgtg
    22081 gccatcgagc cgttgcccgg gacccgcgaa ctgttcgaca tcacgaccgg tactggcgat
    22141 tttctggcga acggcgtggt gagccataac tgctatgccc gcccgagcca cgcttacatg
    22201 ggcctctcgc cgggcctcga tttcgaaacc cgtctcttct acaaggccgg tgcggcggcc
    22261 cggctcaccg aagaactctc gcggccccgc taccagccga agacgatcat gctcggcgcc
    22321 aacaccgacc cctatcagcc gctcgagaag cgcctgcgcg tgacccgctc gatactggaa
    22381 gtcctggcag aattccacca cccagtcgcg atcgtcacca aaggtgcttt gatcgaacgc
    22441 gatctggaca tcctggcggc gctggcgagc gagcgtctgg tcagcgtcat ggtgagcctg
    22501 acgacgctgg acgtcgacct gaagcggcgc ctcgaaccgc gcgcggcgtc gcccgcggcc
    22561 cgcctcgccg cgatccgcca tttgcgcgcc gcaggcgtgc cggtcggcac actcatcgcg
    22621 ccggtcattc cggggctcac cgaccatgag ctcgagcgcc tgctcggggc cgccgccgag
    22681 gccggcgcgc gctgcgccgg ctacgtggtc ctgcgcctgc cgcacgagct gaaggacatg
    22741 ttccgtgact ggctcgcgga acactatccg gaccgcgcgc ggcatgtgat gtcccgggtg
    22801 cattcgatcc gcggcgggcg cgacaatgac ccgcagttcg gcagccgcat gcgtgggacg
    22861 ggcgccgacg cgcaattgct caggcagcgc ttcgatgtcg cattgcgacg actgggcctc
    22921 gagcggcgca tgggcttcga gctggacacc acgcggttca ggcggccgga cgcgcgcggt
    22981 cgccagatgc cgcttgcgct ctgaaggcat ttcgtcgcaa tgcgggaacc gcccgccgca
    23041 gggctggtca gaacccgata cccaccccat ggcatactct gcatacccat gaagtccagg
    23101 gcggctaagc tgataggtgt cgcggggctc ctcagcctct gcgggtgcat gcaggcgcgc
    23161 gtcgaagaat cgcgcgagct gcctaccagg atcagtgggg acgagggagt cgtcgtgctc
    23221 gcaaagcccc aggtcgaggg cgcagggacc gaggcgggat tcatggactg tgtgggcgcc
    23281 aatctggccg gcggcacggg cggactgcgg gtgcacggca acgacgaatt cgtcgacgcg
    23341 atgttcccct ggttcgagcc gagcaccgcg ccctcacgtc ccgaagctgt ctccgcgttg
    23401 ctcgcgcacc cgggcgtcag cgagcgcgtc gccgcgagcg gcgtgcgtta catcgtctgg
    23461 gtcgatggcg cgacccgccg caccgacggc ggcggcagcc tggcctgtgg tgccgccccc
    23521 ggtgcagcag gctgtatcgg cttcggctgg tgggagaagg aatccgatta cgaggccacg
    23581 atctgggacc tccagcaggc caagtcggct ggcagtgtcg gcaccaacgt gaccggcact
    23641 tccgcgatcg tcggcgcggt gattccgctg ccgttcatag cgcgcgtgca gggtacggcg
    23701 tgcaatcgcc tggccacgca gctgcgcagt ttcttcgccg gtgacggcct ggcagagccg
    23761 gtcggcaccg acgacgcgag gtgagtcatg cgcctgtcac agggatggat accggtactt
    23821 gtcgcggcgc tgctgctggg cggctgcgcg acccagcaga gcggcggctc atccggcggt
    23881 ggctcgtccg gcggcggctc gtccgacggc ggctcgtcgg gtagcagcat gccgtcgggc
    23941 ggtggctcga caggcagcgg tatgccgtcg ggcgacggct cgacgggtgg tgggatgtcg
    24001 atgccatcgg gcggagttcc ggacaccggt cagccgggca ccccgggcac cgcgggtgac
    24061 gcggccgggc agccggacgg ctccggcgag caggagggcg ccaccggcgc agctggcagc
    24121 gaaggcgcgg cgggcactgg tcccgagctg ccctcgggca ccgcgcagac tccggaggaa
    24181 cggcgcgccg ccgtggatcg ccggctcgat gattcactcg gcaccttcga cgagcgcatc
    24241 cgcaaggaac aggaaggtgt cgccaaggag cgcgaaagca atgccgcgag cggtgcgggc
    24301 gccggcgtgc tcgtcgcgca aacgggcagc gagtcgggcg ggcgtccggg tgacctcaag
    24361 tccgaccgcg attcggcttc aacccagggc ggcagccagg gcagccaggg cggccaggca
    24421 ggcgcgggct cagagggtcc cgcgcgtacc agcgccggta ccggtggcag cggcaaggcg
    24481 gcggtcgaac gtgcagacgg cagtgacgac gacatcgtcg cgcgccgtct gcgtcgcgca
    24541 gccgagcagg aaaccgatcc ggagctgaag gaaaagctct ggaaggaata cgacgagtac
    24601 aagcggagcg tgcggggcgg atgagcggtt cgatgcacga cgtagtgcgg ctgttgcgtc
    24661 aggccggcgc cctggcgctg cttgccgcgc tcgcggcctg cgtgacgcag gagacgcgac
    24721 cgctgccgaa gctcaatccg gtccaggcga ccgagcagat cccggaggcc gaactgctcg
    24781 acgtcggcat tcgcgctttc gatgccaaca tccccgagca gtacaaggac gacgaggaag
    24841 agcttgccag gcgacgcatc ttcccggagg tgcgccaggc cgagtccgct tatctgcccg
    24901 gactgctnnn nnnnnnntgg accgccgggt cgaggcgatc gttccgatcc ttaacccgac
    24961 ggtgcatgag cagattctcg accagatcat ggttgccaac ctcgccgaca accgccagag
    25021 ctggaccgtc ctccccgacg gcacgtcggt ccgcgtcgtg ccgccgaatg cgacggtcga
    25081 cgtggtggtc gacggcagga tcatcggttc cacgggcgcg cggctcgcgc tcgttgtcac
    25141 cgtgaccgat tccatgggtc gggtgtggat caaggacaag gcgtacgagg gcgaggcgga
    25201 cctcggttcg tacaagaccg acgccgcgct gcaggcgcga gaccctttcc agaacgtcta
    25261 ctcgcagatc gccaacgaca tgctcgccgc gcgggctgcg ctcacgtccg ccgacctgcg
    25321 cgagatccgg cgcgtcaccg aactgcggtt tgccggcgaa ttcgcgcctc aggccgtcca
    25381 gggctacctc tcgaccgaca agcagggcat ctcgcgcgtt gcgcgcctgc cggccgaggg
    25441 tgacccgctg atggaacgca tcgaccgcat acgcgagcgc gacctgtcgg tcatcgatac
    25501 cttcgacggc taccacgcca gcttcaccga caaggtcgag agcacctacg gcaacttccg
    25561 gcgcgtcagc caggaagaga tcgaaaagga agaccgcgcg cgtgcctcgg cgcgcacccg
    25621 gaccgtgctc ggcgccgcgg cggtgctcgc gagcgtactc atcccggacc agtgctcggg
    25681 gggcgactac acctgccgca acgtgcagag cgccgcgcgg acagccggtg cggtcggcgg
    25741 cgttgcgggc gtgatgtcgg gcatcaagaa atattccgag gcgaaggtgc atgccgaggc
    25801 attgaaggaa ctcgcggata ccttccgcgc cgaggccgcg ccgctcgtcg tcgaagtcga
    25861 gggccgcacg ctgaagctga ccggcaccgc cgaagagcag taccgcgagt ggcgcgcatt
    25921 gctgcaccag atctacctcg aggaaaccgg cggtgtcgtc gccggggcgc cgccgcagga
    25981 tagtcccgcc gtcacgccca gcgagtgagt acgtgtccac ccaatacgaa cctggttcgc
    26041 ggcgatcgcc tgtgcggccg gttcacgctg atggccaaga tcggcgtcgg cggccattcg
    26101 gaggtctggc gcgcgcgtga tctcgtgcgg cgcgaggatc tcgcgctgaa ggtcatgaag
    26161 cgcattgcgg ccgcgccggc cgatgcctgg gcgctgctgc agcacgagta cgcggtcacc
    26221 cgcgaactgg gccatcccgg gatcctcagg gtctacgaac cgctggattg cgacggcacg
    26281 ctggtgctgc cgatggcgct cgcgccgggc ggcgatctcg gcgcgctgcg cggcgagccc
    26341 tatacgcgca tcgtgccggc gctgatcggg atcgcgcagg cgctcgagca cgcccacggt
    26401 cgcggtgtcg tgcatcgcga cctgaagccg ggcaacgtgc tgctcgacgc cgaggggcac
    26461 acgctgatcg ccgatttcgg tgttgcgtcg ttctcgggcg gcagtccgtt cgcggaccat
    26521 ggctcgccgt tcagcgcgag cccgcaggcg ctgcgtggcg agccgcccac gccttccgac
    26581 gacatctacg gcctcggcac gctcgcctat gaactgctga gcggctatcc gccgttctac
    26641 ccgcgcttcg agacgcggcg cgtgctcgag gagccggtcg ccgaccttcg gcccatacaa
    26701 cccgcgccgc cgcgcctggt cgcgctcgtg atgcgcatgc tggcgaagag ccgcgaggcg
    26761 cgcccggagt cgatgcgcgc ggtgatcgat gaactgcacg cgtcgctgca cgacacagtg
    26821 acgatcgaga ccgaagcgct tgccgggtcc ggcaacggca ccgaagtcat cgccgcgtcg
    26881 aatccgacaa cgccgatcgc gggctcgccg ggtgatgcgt ccggcgacga ccgtacgatg
    26941 gcgatcgagt tgcccgagcc gatgcgcgcg ctggccttgc ccacgtccgg atcggcggac
    27001 gagatcgtcg ccgcgccgca cgatgccccg gcactgatcg agatcgaacc cgcagctgcg
    27061 ccaagggagc gacgaatcgg cacctggctc gcactcgcgg ccgtggctgc ggcgatcgtg
    27121 ctcgtgttct tcgtgatgcc gcgactcgcg ccgcgtactg ctgcgccatc ggcgggcgtc
    27181 gatgcacagg atgcacccgc cgaagccctg gatccggcca cacccgccgc ggatcccgtc
    27241 gaggcgctcg cgacgctgcg cgcgggcatc acgaagcggc tggctgccgt cgagacgcgc
    27301 gcggccgcgg tgtggggcgg cgatgatttc gcagcggcga agcaggccgc ggagcatgcc
    27361 ggcgtgcttg ccgacgatgg tgagatcgac gccgccgtca cggagttcac ggcgctgggc
    27421 aagcgcctgg ccgatatcga ggcgcgcgcc ccggacgcgc tcgccgcaga gctgggtcgc
    27481 ggcgatgcgg cgctcgaggc cggtgaaccg gcacgtgcgc gcaacgcctt cgaactcgcg
    27541 cagcgcatcg atccgcagaa tgcccgcgcc gccgccggcc tcgcgcgcgc ccaggcgctg
    27601 gagatcgcga tgccgctgct tgcccaggcc gccaacgcgg cagcgtccgc gcgccacgcc
    27661 gaagcggccg aactctacca gcaggcgctc gcagcggatc ccgccaacac gcaggcgcgc
    27721 gagggactgg ccgctgcgcg cgcggcggtt ggtgccggcg agtacgcgcg tacgctggca
    27781 agcggcttca gtgccctgca ggcgggcgat ctcggccgcg cgcgcagcga gttcgaacgc
    27841 gcgcgcgcca tgcagccgtc ggcgagtgct ccgcagcagg gcctcgagca ggtggcggcc
    27901 gccgagcagg cgcgcgccgg agacgaggcg cgcaggcgcg cggcgtcgct cgaggcgtcc
    27961 gaacgctggg gcgaggcact ggcgatctac gaggaggtgc tcgcgcgcga tccggcgctg
    28021 cagttcgcgc aggcaggccg cgatcgggcc gcgccgcggg ccgagctcgc gcgacgcctg
    28081 cagctgatca tcgacaatcc cgaccggctc gcggccgacg ccgtgcgcag ggagaccgag
    28141 gagctgctcg agcttgcgcg cgccatcgag cccggcggcc ccgtgatccg ctcgcaggtg
    28201 agccgcatcg aactgctgct gccggcctac gaccaggtcg tgcgcgtcgc gctcgagtcg
    28261 gacaacgcca ccgaggtcac catccagcgc gtcggcgcct tcggcacttt cctgcggcgc
    28321 gagatcgagc tcaagccggg ccgctacgtc gtcaccggca ggcgcgatgg ctaccgcgat
    28381 atccgccgcg agatcacggt cgccccgggc accaccacac agaccatcgc cgtgagttgc
    28441 atcgagccga tctgatggaa accgagcgca tatcagtcac cgagccactc gggcagcgcg
    28501 aacttgccgc gccgctgtcg atcggtgccg cgggctcgga catcgtcgtc ccgggtaccg
    28561 acgcgactcc cgcgctggtg ctcgatgtcg acgaggggct gtggcgcgtc acaccggcgc
    28621 aggacgcggg tgcgcggctg aacggcgagc gcctgcgcga ggcgcgcgac ctgcgcgccg
    28681 gcgacgtgat cacgctggcc gatgcgcagg tcatcgtgcg tgccaccgcg ccgtgcgcca
    28741 tcgaggtgct gcacctcgcc ggcaacgata cggtcgcgcc gctcgtggtc gccgaggagc
    28801 gcgacggcgc gctcgatgac gaagacatcg agatcggcgc cgtcgcacgc gaggtgctgg
    28861 ctctcgatga cgaggcgccg cgcgccgcgg gcggacgcat cgcccgccgc gtcgccatcg
    28921 cagctgcgct cgccgtggtc gccgcggtgt tcgcgctgtt tgccagcgtg cagcgtgtgc
    28981 cgctcgtgct cgatccggtg cacgcgcgtg tcggcgccac cgacacattg atcgactggc
    29041 actcgggcgc gacgctcttc gtgctgccgg gcaagcacca gctgcgtgcg agcgcgcccg
    29101 gttatgtggc tgtcgagcgg gaggtgaccg tgacccgcga gggtgcggtg cccgtgcgcc
    29161 tgcggctcgc gaaggaaccg ggcgtgctcg tcatcgacac cggtggcgtg atggcgagtg
    29221 tcgccgtcga tggcgccgag atcggccgcg tgccgggcga agtgcgggtc gcagcgggca
    29281 atcgcacgct gaccctgtgt gcggagcgtt atctcgacgc cgtcgtcgag gtcgaagtcg
    29341 agggcctcgg cgtgcgccag gacctgacgg tcgcgatgca gccgtcctgg ggcacgctcg
    29401 cgctcacggt gaacacgccg ggcgcgatgc tggcgatcga cggggccgcg cccgctgccg
    29461 tcgaaccgcg gatcgaactg ccggccggcg tgcaccagct ggcgatttcc gccgcgggcg
    29521 cgaaatcctg gcagagcgcg atcgtgatca aggccggcga gacgacgacg atcggcccga
    29581 tcgacctggg tgcgcccgat gcacgcctgg tcgtgcgctc cgtgccctcg ggcgccgatg
    29641 tctcgctcga tggcgtctat cgtggccgca cgccgctcga catcgccgtg gcgcccggca
    29701 gcatgcacga tgtgctggtc gcgcgaaccg gccacgcgcc gtggtcacgc agtttcagcg
    29761 ccgcgtcggg cgagcgaacg gtgctcgacg cgcgactcga gccggtatac gtctcgctga
    29821 ccgtgcgcgg cgaaccggcc gatgccgaag tgctgatcga cggcgtgtcg aggggccgca
    29881 cgccgctgac gctcgatgtg ctcgccggcg agcatgcgat cgaggtgcgc aaggtgccgc
    29941 tcgcctcgca cgagtcgagg atcacgctcg caccgggact cgcgcgcacc atcgaatacc
    30001 agctgaccgc ggcgggacga ccctcgagcg cgctcgccgc cggcacgcag ctcggcacca
    30061 gggtcggcta cgtgctgcgc ctcgtgcagc cggcgacatt ccaggttggc agcgagcggc
    30121 gcgagcaggg ccggcggccg aacgagacgc gccggcaggt cacgctgacg cgcgcctact
    30181 acatcggggc gaccgaggtg accaatgcgc agttccggcg cttccggccc gagcacgcct
    30241 cgggccatgt caacaacacc agcctcgatc tcgacgcgca accggtcgtc aaggtgacgt
    30301 gggaagatgc cgtgcagttc tgcaactggc tctcggaaca ggaagggctc acgcccgctt
    30361 acgaaagcag ggacggcaag tggcaactca agaccccggt gtcgaacggc ttccggctgc
    30421 cgaccgaggt ggaatgggag tacgccgcgc gttacgccgg ccccggggcg ctgcagcgct
    30481 actcctgggg caatgcgctg ccggtcgctc ccgcgaacgg caactacgcg ggcgcggaag
    30541 cgatcggcac catgagcccg atcctcgaga cctaccgcga cgatttcccg gtcgccgcgc
    30601 cggtcgggaa attcggcgcc aaccggctcg ggctgtacga tctgtcgggc aacgtgagcg
    30661 aatgggtgca cgattactac gtctcgctgc cggaaggcgc ggcacagacc gacccgttcg
    30721 gccccgcgca gggaacccgg cacgcggttc gcggctcgaa ctggcgtacg gccacggtgg
    30781 ccgacctgag gcttgcctgg cgcgacggcg ccgagggctc gagccaggtg atcgggttcc
    30841 gcgtggcacg ctacgccgac ccctgagacc cacgagcacg gggatatgcc catgagaaac
    30901 atctggcgcc agatctggat catcagcttc gcatcgctcg cactctgggc cgcggtgctc
    30961 gccatcgcgt accggctgcc gtccgcggtc gcgcagtcga ccgaggaacc tgcgccttcc
    31021 gccgagccgg caccggctgg cgaggcgcca cccgatgagg cgggcatgat cgacgagggt
    31081 ccgcagatcg gcgcgcctgt caccgaacgc gaggaagtgc cgccggagct gcgccagtcc
    31141 gccgacaaca acatcagttt tcccgtcgac atctgacact gaaagccacg catgcccatc
    31201 cagcgcagat accccagcga attcgtcttc agcctgttcg cactcgcgat cacgatcgtg
    31261 atcgtgcacg gcgtctacgt gcttgacatc aggccgaagg cgcaggcgat cctcgaggag
    31321 cagcgtgccg gcatgctgaa ggatccgaac tacatcgccg aacgttcctt ctacgtgctg
    31381 atcaaggatt acgagcagga gagcgagatc atcctgttca tctgggcgct ggcgatcatg
    31441 ggctacaagg cgcggcacca gaagcgcgag cgtgagacgc tcgagggcga tcctctcaag
    31501 ctgcccgagg gcatgaagat cctgcccgag gactcgcgcg agtacacccg ccagctcgag
    31561 gcgctgccgt ccgaaaagcg ccagttgctg ctgccgcgag cgctgctcgc cgcgctcgcg
    31621 cgcttcggtg ccacgcgcag cgtgcaggac gtgtcgaacg tcgcgcacgg cacctgccag
    31681 tcggaagccg agcggctcga ttcggagatg tcgatgctgc gctacatcgc ctgggcgatt
    31741 ccggccatcg gcttcatcgg taccgtgcgc ggtatcggcg atgcgctgtc acaggcgcac
    31801 aaggcggtcg gcggcgacat ctcgggtgtc accgagggcc tcggcgtcgc gttcaattcg
    31861 acgttgatcg ccctgctgct gtcgatcttc ctgatgttcc tgctgcacca gctgcagctc
    31921 gcccaggagc ggctggtgct cgacgccgag acctatctcg atacacggct catacgcaac
    31981 atgcaggcga ggtgacggag gcatgacgac gctcggcgtg tcgatccacg atggcgctct
    32041 ggcgctggcg ctggacggac gcgtgctgtc ggtcgcgccc gcgatcgtgc ggcgtgcggg
    32101 tggtgccgcg cccgccggca cgcccgcgct cgatttcctg cgcagtcgtc ctgccgaggc
    32161 ctcggcgcgg cactggagcg acatcgccgc ggaaggagcc gccgcgcgcg ccgcgttgcc
    32221 gatcgcgctg gcggaactgc gcgagcgcct gcgcgaggcc tgcgcagtgg acggcgccgc
    32281 cgcgggctgc gatgcggcct ggttcgcggt cggcgcggcc ttcccgccga gcgcattgtc
    32341 gggcgtgctc ggcgcggcgc gcgccgtgcc gctgccgttg cgcggcttcg tcgatgcggc
    32401 ggtcgcgagc gtcgcgccgc tcgggctcac gcgtcccgcg atcgtgctgg aagtggggct
    32461 cttccacgtc gccgccacgg cggtggagtg cgggcccacg gtgcgtcggc gccgctccat
    32521 ggtgagccgc ggcggcggcc tgctcgacat ctaccagtcc tggctcgacg tggtgagcgc
    32581 cgcgatggtg ctgcgcacgc gcttcgatcc gctgcacgac gcggctatcg agcagcgcct
    32641 gttcagcgag ctgccggcac tcgcggccca ggcggaggag agcggtgtgg ccaccgtcac
    32701 gctcgagggg cccggcgggg agccgcacgc cgtcgagatc gcccgcgatc agttggcggc
    32761 agcggccgcg ccgcgttacc gcgaactcga acgctgcctg cacgagttgc gccgcgcggg
    32821 cgcgccggtc gcactcgtgg tgcccgcggc gctgctgcgg ctcgccggcc tgcgcgagcg
    32881 tttcgagagc ctgccggatt gtgaactcgt catcgtccct gacggctggg cgggcgcggt
    32941 cgcttcgatg ttgccggtgc cggccgaggg tgccgatgcg ccggtacgcc tgctgcgccg
    33001 tctcgatgcg ctcgaggccg cgcctgccgg cgaagcgccg cgacgcgagc gcctcgccac
    33061 ccgcggcggg gcggtcgatc cgacccatgt gctgcatgcc gggcgcgccc ttgcgcccga
    33121 tgaccgcggc atcagcgtcg gccgtgacgg taacgacgtc gtcatcagcg tgtcgtccgg
    33181 cgccgcgggc gtgtcgcggc gccattgcac gttcctgcgt ggcgatgacg gcgtcatgct
    33241 catcgaccac agccgccatg gtacgtgggt gaacgacgag cgggtcgcgg ggcgcgcgcg
    33301 gctgcgcgcc ggtgacgagg tacgcatcgg cgagcccggc atcgtgttcg cgctgatcgc
    33361 ggtgggagcc gaaagtgcgc aagcgtcggc agactgaggt cttcagcctc tcgttcctcg
    33421 actgcatctg ctgcggtttc ggcgcggtcg tgctcttcta caccgtcgtc agtgcgcaat
    33481 ccggcgtaga gcgcatacgc aagaccgagg atctcgccgc cgaagtccgc aagctggaag
    33541 acgaggtgct cgagggcacg cgcaacctcg tcgtgctgcg caatacgctc gagaagacga
    33601 gcagcgagac cgcgagcgct gcgtcgcgcg ccacgcggct gatcgaggag ctgaagcagg
    33661 cgcgcgacca gggcccgatc atcgacaaca ccaccctcgc gcagcgcgag cacatcgaga
    33721 agctcaaggc cgacctgcgc tccctcgagg agagcacgcg ccggctcgag gccgcgagca
    33781 aggacgtcgc tcccccgggc gatgcggtga aggtgacgcg cggcgccgac cgccgctaca
    33841 tcaccgggct gcggctgcac ggcaagcatg tgctggtgct gctcgattcg tcggcgagca
    33901 tgctcgacga ggacatcgtc aacgtgatcc gcctgcgcaa taccggcgat gcgacgcgcc
    33961 gtgcggccgc caaatggcgt cgcggcgtcg ccacggtgag ctggctcgcg ggccagattc
    34021 cccccggcag ctcctaccag gtctatcgct tcgataccaa accggagccg gtgctcgccg
    34081 ataccgccgg caactggctg aagggtggcg accaggctac gggcgaccgc ctgctcgagg
    34141 cgctggacga ggtcgtgccg cagaacggca ccagcctcgt gaacgccttt cgggcgatcc
    34201 gccagctctc gccggcgccc gaccaggtca tcctgattac ggatggcctg ccgacccagg
    34261 gcgcgtcgcc accggccgtc cgccgctaca tcgacgccgg cgggcgcgca cgcctgttcg
    34321 aggacgcgat caaggcgctg ccgccgcgca caccgatcga tgtcgtgctg ttgccgatgc
    34381 gtggcgacaa tccggcggcg catgccttct ggaggctcgc ccgcaacagc ggcggcgcct
    34441 acctgatgcc ctcgaaggac tggccgtgag ggcgccgcga tgaagctgca gcggcgtgac
    34501 accgaggtct tcagcctctc cttcctcgac tgcatctgct gcggtttcgg cgccatcatc
    34561 ctgctgctgg tgctgtcgga gttcggcgag ccgatcgtga tcgagaagag ccgcgagcag
    34621 ctcgacagcc aggtgctcgc gctgcagcag gaattgaacg tgatccgcgg cgagaccgag
    34681 gtgctgaacc gggagatgca ggcgcgcgtc gacgagttgc gcaaggagag gctgaggctg
    34741 gcgcgcgtga gcggcgaagt ctcctcgatc cggggccagt acgaggcgag ccgcaaggaa
    34801 gccgcggtca cgaacatcat cgagggcgag ctcgtccagg cctacgaatc gctgagcgag
    34861 gagctgaagc ggctgcagaa ggtctcgcgg cggcgcccgc cgaccgaggc cgtcggcggc
    34921 atcccgatcg acagcgagtg gatcatcttc gtgatcgaca cctcgggcag catgctgtcg
    34981 atgcactggg agggcgctca gcaggtgttg aaggaaatcc tcgacatcta cccgcgggtg
    35041 cgcggactgc aggtgctcga tgacgagggc aagccgatgt ttgccggcac ccgcggacaa
    35101 tggctgcagg acaatcccca gcagcgcacg cgcatcatcg acaagatgcg cggctggcgg
    35161 gcattctcgg actccgaccc ggtgcagggc atccaggagg cggtggccac ctactgggct
    35221 gccgacaagc ggatcagcgt gtatgtgatc ggcgacgagt tcaccggcga ctcgatccag
    35281 cgcgcgctcg atgccgtcga caaggccaat ccgcccgatg ccaacggccg gcggcgcgtg
    35341 cgcatccacg cgatcgggtt tccggaggcc cgcggcatgc cggcgtttac aagtatccgc
    35401 tttgctgcct taatgagggc gatgtgcgac cgcaatggcg gtacgttcgt gccggtgacg
    35461 aacgaaaagt cctgcgcgcg ctatatcgag gtcttcggta cacgccaatg catcagcgaa
    35521 ggctagcgaa ggtaatcggc ccgttacttg tccggctggc ggtcggcgcc ggggcactgc
    35581 tgctcgccgg ttgcggcagc gtcgagattg cacccgaacc ggtcatcccg cggccgctcg
    35641 tccggccgat cgcggccacc acggccgtgg tgctgggcgg ggagcagcgt aacttcaagc
    35701 acaccgagac ccgcgccggc gtcgactggc tggtcgccct gggcgccggg cacaaacggc
    35761 tggccgaaga ggtcttcggt gcgctcttcc ccggctgcgt attcgtggac gaactcgagg
    35821 aagcgcgggg cattgccggc ctcgacgcgg tgttcgagcc gcgcatgctg cagtattcgt
    35881 tcgcgaccgc caacgaaacc ggtgccggtt actacgcggt gaccatcaac taccgcatca
    35941 acatctacgg gccggacctg aaactcgtcg attcgtacac gctcacgggc tacggcaacg
    36001 accgcgacaa ggcgctctcg agcagcgagc cgctcgccgg cgccacgcgc gcggccatgc
    36061 gcgatgcggc ggcgaagttc ctcgtgcagt ttccgcagca gcccctggcc cagcggctcg
    36121 ccagcggcga accgctggtc gcgccggggg acgccgctcc cggcgaggag atcaaccggg
    36181 ccgacgcggc acgccagatc gaggccgtgc cagtgctcgc gccgaagaac gagccggtct
    36241 cgccgcccgt ggattcgcag ccgaaggtcg tgcagttgcc ctagcgaggc gccagtcagc
    36301 ccgtatcgtc cgccacctgg ggccggcgct tcagcatgcg ctcgaccagt gacacctccg
    36361 gcgtgtcgac cggctcaccg ttgacgaggc gaggcgcata cacggccttg cgcaccgcgt
    36421 acgcaacgct gtcgtgcatc gattgcgggg caacgctcgg cacggtggcg atgtcggccg
    36481 tgccaccgtc ggccttcacg gtgaacttcg cctcgatcac gaattcctcg tagtcgttga
    36541 cgtcgccgcc cttgaagcgc gcgatggacg ccgagggtgg ccggtagcgc agctgccggg
    36601 gggcatcgac gagggctgtc gtgcccgctt cctcgagggc ctgccaggct tcgcggtagg
    36661 caagcagcgc accgcggatg ttgccgccga tcagctgcca gtcggcgagg tcgaccaggg
    36721 tttcgccgcg cagcgcggga tccggcggct cggccttgtc gatcgccgca agcgccagct
    36781 gcagcgaacg ctcgccttcg ggattgaggc gggacggcga ctggctggcg ccgggacccg
    36841 agctgaactg aagcgggtcg ttgaccgccg actgctcgac cggttcggca ccgaaaaggt
    36901 actcgaggcg gtaggcgcgc gcgagcccgc gcagcggcat cacggtggcg atactgcccc
    36961 gccccgagga cgcctcggca atgcgcagcg cgcgatagtg atgcgcgcgc gcggtcgtat
    37021 agcgccccac gtactcgtac cagcgggcca ggtagtcgta cgcgggcagc atgcgcggat
    37081 cgtccctgcc gtaagccgtc tccgcgatgc ggaaggcgta caggtgctcc cgctcggcct
    37141 cggtcagcct gttgtcctga acataggcgt cgatcagcgt gaacaggtat tcgagctgct
    37201 cgaggttgta caggccgtcg atgttgcgcg acaggtcgat cgctgcctgc agggcggtcg
    37261 tggcggcagc aggctggtcg ctgcgcgcgt aggtcatgcc gaggccctgc agcggccgca
    37321 gcagcgcccg gtcggtcgcg gtcgcgttgg ccttggatcg cacgatctcg agcgcgcgcc
    37381 ggtaggacgc ctcggcggcc gagaactggt tgaggcgcag ctgtgtcgtg ccgagattgg
    37441 tgagcggtgc ggcgagcgcg cggtcatccg ctccgtactg ctcgcgggtc gcggcgacca
    37501 gttgctcggc gagcggcagg gcctcttcgt agcgtcccgc gtcgaacaga gtgcgaaact
    37561 gctgatagat ggccgtgcgc tcggcgtcgg tgacggcacc tgcccggggc gcgagcgcag
    37621 ctccgcaggc gacgatcaac agcgccgcga gtgccgattt tcctcgcatt cgatgctcca
    37681 gctgctaccc gcaagatatc attcctgcag gcgggggaca taccatgaac gctgtgcaca
    37741 agaccgccat ggtcgccggg gcgagcggtc tcgtcggtga acggctgatt tcggtgcttt
    37801 gcgagtcggg cgactatgcg cgcgtgtacg ccgtcacgcg ccgcccgctg gcacgtgacc
    37861 acgcgcggct cgccaatcgc atcgtgcgct tcgagcagct cgaggcgcag ctgcgcggca
    37921 tcacctgcac cgacgcctac tgttgcctcg ggaccacgct caaggccgcg ggctccgagg
    37981 ccgcgttccg gcacgtcgac ttcgacctgg tgctcgcctt cgcgcgcgcc gccaaggctg
    38041 cgggggcgca gcgcttcgtg ctggtgtccg ccgcgggtgc cgatcgcggc agccgctcgt
    38101 tctatctgcg ggtgaaggcg gaggcggaag cggccgtcat ggcgatcggg ttcgccgcgc
    38161 tcgatatcat gcagccggga ctgctgctcg gttcgcggcg cgggctgcgg ccgctcgagc
    38221 tgctggccca gtggacgctg ccggcaatcg caccgctgat gatcggcccg ctgcagccgc
    38281 tgcgcccggt gagcgcgacg accctcgcgg aggccatgct gggcgccgcc cgctccgggc
    38341 gacgcggtgt catgcgtttc acctggcgcg cgatcacgac gcttgccgcg gggcaggcgt
    38401 cgcgtcgcgc cggctgatta cttcttcttc gctaccttgc gcctggcgac cgggcgcttg
    38461 gcgaccttct tcttggcagc cgtcttcttg gtggccttct tcttcgcctt gcgcttcggc
    38521 aactcgctgg cagcggtgct gcgcaggtcc gctgcgtgcg cgcgcttgta gctctggatg
    38581 tccttgaact tgccgctctt gtcgcgcacc gcgtaaagct tggtgccctt gctgctgcga
    38641 tgtgtcgtgc gtttacgttt cgccatgaga cctctctccc gagtgtatct ggaaacgcgg
    38701 caacgctaat acgctggacc gcgcagcgtc aacgctcgac gatgcgcgcg accggggtcg
    38761 agtgcagcac gcgacccgcg tgtacgagga atccgccgac cacggcgagc agtccgctcg
    38821 gcactccggc cagtgtcgcg aggccgaccg cggcagccgc agtcgaggcg agcagcgaca
    38881 cgccgccgcc tgctgccagc cacagcatgc cggtgatcat gaacgcgcgc tcgaggaaga
    38941 tcagcgcgtc tccccagcgc agcgccggcg gcagcaccag gtgcagcgcg aaagcgagcg
    39001 tgacgattgc cgcggtgatg cggaatgcgc gcgcgcgcgt cacgccccgc agtgtcaccg
    39061 ccagcggcag tgcgatggcg agaccgaggc attcatcgag tgtgagccac tggccgacga
    39121 agacgacgcg ggcggcgagc gagagggccg cgacggtgag gaagatgcgc atgacgctgt
    39181 ccggcacgac caggcgacgc acggccgagg cgaccaccag atagcccgcg caactggtga
    39241 gaatcgcgat gaagtcgtag tcgaggtcgc gcagtggctg catgcccgcc caggcgtggc
    39301 cctggttgag ccgtggcatg aacggtaccg cgtgcattgc gaaccacagc gccagcagca
    39361 ccagtgcgat cggttcgggg cgtgcatggc gcagggcgat cgccccgatg ggccaggggc
    39421 gcgaacgcag cagggcgccg gcgatggcgc ccaacgcgcc gccgctgctg ttcatcgcga
    39481 cgtcgaccag attgggtacg cgtgagcgcg tcacgtgctg cagtacttcg atcgtgacgg
    39541 acagcgcgcc cgcgagcgcc acggcacagg cgaaggcgag cggcgcgcgc cagcgcactg
    39601 gcgcgaggta ggcgagcagt gcgccgacgg gcaggtagga cagcaggttg ccggcccggt
    39661 cggcgcgcgc cgaatagccc cacggcagga gctggcccgg cccgccgccc cacgccgcga
    39721 tgagccggcc gacgtcgaag cgaaacggaa agaacgacgc gtagaagatg agcaccagcg
    39781 tcagtgccag taaccacgcg gcacggcgtc tcgactgggt gcgctggaag gagacatagg
    39841 tcacggcggc aagcatagcg tgatgcaatt cacatctgcg cgctttgctc tggcgcacgg
    39901 gttggcggat cggtgacacg tgccacaccg gccgggtgcc gtctttcgtt aaattgccgg
    39961 tcatggaccc actccgtgcg tgcctcggca cccgcatggg acgccgcatc gccgtcaccg
    40021 ttgcggttgc cgtgctggcg ccgaccctgc tcgcggcatt cgtgctgttg ctgcattcgg
    40081 cgcgcacgat ggacgcggcg gcggcgcgcg ccctgacgat cgacgcgcag gcgttcgccg
    40141 ccgagttcgg cgcacgcctc gacggcgggc gggcagccgc gaatcgtcac gatgcggccg
    40201 tcagcttcgc cgccgagagt gccgcgcgac tgccggccga cgccgtcgcg acgccgatcg
    40261 gcaccgaacg acgcattgcg ctgctcgacg gcgcgggtcg gctgctcttc agcaccgcgc
    40321 cgctgccgac cgacgtcgtc gatctgctcg ccaactacag tcgtgcaccg gccgccacgc
    40381 gcggggcgct gccgctcgca tggcaggccg ccggagtcga gtggcagggc ggtgccgcgc
    40441 gcctcgagcc gcgcgagccc ggcggaacat cctggtccgt ggtggtcttc aagccgcggt
    40501 ccgcatggag tgccgccgtt gcgccgctcg gcggcgagct ggcagcgctc gccctgttcg
    40561 cgctggcgtc gatggtcatc ggcacgctca cgctggtgcg ccgctacgtg ccgatcacga
    40621 tgcgcttcgc tgccgcgcag ggggagggcg gcgtgggcat cgcgcccggc ccgcgcagcg
    40681 tcgatgccga cgaactcggc gacgcgatcc atgccggcga cctcgcgacc ggccggcttg
    40741 cggagcgcgt ggccgcgctc gaaaccctgg gcgagatcga ccggctgctg ctcggctccg
    40801 ccgagctcga gcagatgttc gacacgatcc tgtcgcgcgt gcgtgcggtc acgcgctgcg
    40861 atggcgtcgc gatcacgctg atcgacgccg actcggcgac acacgggcgc gtctatttcg
    40921 cggcaccgca ggtcgtggac ctgccggtgg tgcgggtcga actcgatccg gacatgctgg
    40981 cgacgctctc gcaggcgcag gagggcatta cgatcacgcg ctgcgaaccg gagcgccaca
    41041 gcctgctgcg gccactcgcg gagctcggtg cgcagttctt ctgggtatgg cccgtcattt
    41101 cgggcgaccg cctggtcgcc gcactgtcgc tcggctatcg cgagccgccg tccgccgatc
    41161 cgtcggtcgc aaactacggc gccgagttcg cctcgcgcct gtccgtggca ctgtcgaaca
    41221 gtgcgcgcga cgagcgcctg tatcgccagg cgcatttcga tgcgttgacc ggcctgccta
    41281 accggctgct gttccgcgac cgcctcgcgc aggagcttgc gagcgcggtg gccggcggct
    41341 cgcgcggcgc gctgctgtat atcgacctcg atcacttcaa gcgcgtgaac gacacggtcg
    41401 gccacggcgc cggcgaccag ctgctcacga tcgtcgcgca acgcctgcgc gcctgcgtga
    41461 aggacggtga tacggtcgcg cgcctcgccg gcgacgagtt caccgtggtg ctgcgccagg
    41521 tcaccgatcc cgacgccgta cgcaccgtcg ccgagcgcat catcgccgcg ctcgaggcgc
    41581 cggtgaacgt ggccggccgc gatcacctgg tgcgcggcag catcggcatc acgctgttcc
    41641 ccgacgacgg caacaacatc gacgagctga tgcgcaacgc cgatacggcc atgtatcgcg
    41701 ccaaggacct cgggcgcggt cgcgcggtgt ttttcgatcg cgcgctgact gtgcgccagg
    41761 tgtcggcaac cggcagcgga ctgtaccgcg cactgcgcca ccgcgagttc tcgctctact
    41821 accagccgca gttcagcatc gacgacggcc gcctggtcgg cctcgaggcg ctgctgcgct
    41881 ggcagacgcc gcgaagcgga ctcaagtacc cggccgagtt cgtgcccgcc gccgaggaga
    41941 ccggcctgat cgtcgacatc ggcggctggg tgctcgaagc ggcctgcgcg cagattgcgc
    42001 tgtggcgcga gcaggggatg tcgccgccgc ggctcgccgt gaacgtctcg gcgctgcagc
    42061 tgaagtgcaa cggcttcgtc gcgaacctcg agcagctgct cgcgcgccac gggctggccc
    42121 ccggcgcgat cgagctggaa gtcaccgagg ccgtgctcgc caaccccgac atcgctgagg
    42181 tgctgcgcga gctcgccgcc cgcggcattc gcctcgccct cgacgatttc ggcgccggcg
    42241 agaccgcgct tgcgcacctg cgccaccatc cgatcagcgt cgtgaagatc gaccgcacgt
    42301 tcatcgagga gatcgcccac gatcccaccg ccgcggcgct cggtgctaca gtgatcgcca
    42361 tggcgcatgc acttcgcaag agtgtggtgg cggaaggcgt ggaaacgatc gagcaactcg
    42421 atttcctgcg cgagcacggc tgcgacatcg cgcagggcta ctacctcgcg cgaccgctgt
    42481 ccgccgccgg catcggcgag ttgctcgcgg gtcgcgcgat cgcatcccag ctgacgaggg
    42541 accgtctcgt ggggtagctg gcgtgatgcg gccgcaaccg ttgctcgcag tgccgctcat
    42601 gttgctgctg gcggcctgcg ccggcacgcc gcgctcctca gcgccgacat ccggcgcccc
    42661 cgtgcccgcg tccggtgaca agacgtcgcc cgcggcgacc gcacccggcc gcggcatcgt
    42721 gctggccgcc gaaggcgtga tcggcacacc ctatcgctac ggtggcgcca gcctccaggg
    42781 gttcgactgc agcggactca cgcagttcgc gcaccgtgcg gccggcatct ccattccgcg
    42841 caccgcggct gcccagcagc gcgcggcgac cgcggtcgca cgcaatgccc tgcagcccgg
    42901 cgatctcgtg ttcttcgtga ccggtcggca gggcgtggac cacgtggggg tctatgccgg
    42961 tggcgggcgt ttcgtgcacg cgccatcgag cgggcgtgtc gtcgcctacg cctatctcga
    43021 cgatccctgg tacgcgcagc gctacgtggg cgcgggccgg tactggacgc acgccacggt
    43081 cgctgcgaca ccgcgctcct cgccgtgata cgttcaccgg catgccgggc cgcacccttg
    43141 ccacgctgac tgctgcagta ctggtcaccg ccatgacaac ggcgtacgcg gccgatgccc
    43201 cgactgtcct catcaccggc gcgaatcgcg ggctcggact cgagttcgcg cgccagtacg
    43261 ccgcgcgcgg ctggcaggtg atcgccacgg cgcgcaggcc ggccgaggcc acggcgctcg
    43321 cggaacttgc cgccgcgcac cccggtgtgt cgatcgagac cctcgacgtg accgatggcg
    43381 ccgccatcga ggcgctcgcg atgaaattgc ggggccaggc gatcgacgtg ttgctgcaca
    43441 acgccgggat caccggcgcg cgggccggcc acgggctcgg cagcttcgac tacgacctct
    43501 accggcgcgt gctcgaaacc aatgcgatcg gcccgatgcg cctcaccgag gcgctgctcg
    43561 acaatgtcgc catgagccga cagaaaaaga tcgtgacgat ctcctcgagc gagggttcga
    43621 tcacgcgggt cgatgcgccg cgtggttact ggtaccgctc gagcaaggcc gcggtgaaca
    43681 tgttgatgcg caatctcgcc ttcgaggtgc ggaagcgggg catcacggtg gtactcgtca
    43741 atcccggccc ggtcgacacc gacatgatga agggcgtgcg catgccgctg cagccggtcc
    43801 cggtcgcggc cgcgacggtg atccgcatca tcgatggcgt ggcactcgaa gagaccggcc
    43861 ggttctggga ctaccagggc ggcgagttgc cctggtagcg gtgcctggat ttcggccttt
    43921 ttcggtcagt gagcgctgcc gccgcgttat gtcgcgccgc agcgccgctt cgccctcgtc
    43981 tgcgaacgag tgcacggatt gcacatacga gccgccgaat cgcttgacgg tgcgcagcga
    44041 cacttccaga ctttatgtgt gatcaggcaa acccgccgcg aggcggggac gcaaagccac
    44101 cggtcttggc gcgcgccgag acagcggggt tgcctcgagg cgccgcatac cgggccaagg
    44161 cttcgagact ccccggcctg gcgtccctgt ccgcaagggt gttccgggga aatacagatg
    44221 caggttacgg ctgaagccac ggctgagcag ggcgcagagc cagcgcagta cgtcatcaat
    44281 ctgtgcgcgt ccacgacgcc gatggcgctc gcgcagccga aatccgccgc gctgaaacgc
    44341 ttcactttct tcgtcagccg ccgccgcgag gatggccgcg aacgcttccg tctgcacatg
    44401 ggctacttcg cctccacgga agaggcggac gaatggctcg gcgtcgtgcg cgagctgtat
    44461 ccggccgcgt gggtcggcga ggcgccgggc aggaaactgc gcgccgctgc gcgcgcggcc
    44521 acgccgcccg ccgctgagcc ggtccggccg cccgtgccgc cgattgcggc ggcgcaggcg
    44581 ccgaagccgg agcctgctgc ggcccgcgaa gtcgcacctg cgccacccgc ggcgacatcc
    44641 gccgcgccgc cggagatggc ggcactgtcg aacgttcgcg aagtgctcgc tctgctcgag
    44701 gacgacccac ccggcgccag cgacgtgcgc aaggcacccg cggccgctgc ggacgatgcc
    44761 acggacgcca cccgcagcat gccggtgcag acactctatg tgccgccgcc gccggtgctg
    44821 ccggaacaca tcccgaccgt gcaggcgctg accccggcgc cgatggcggc caggcccgaa
    44881 tcgcgcccga cgccgaaacc ctcggtgccg aaagctgcgg cgccgaagcc cgcggcgccg
    44941 aagcctgcgg cccaaagacc cgcgccgccg aaagtcgccg cgcctgcgcc gcaggctccg
    45001 ctcgccaggc ctgcggtgcc gacgcagaag atcgcgccgc ccagggtcac gccgccgcca
    45061 cagccggcag cggccagctc atcgctctcc gacacacagg tgctgcgcgt cctcgaggag
    45121 cggcgcgccg acgccggtgc agcgcccggt ccccagaccg ccgaggagcg tgcggcgcag
    45181 gcggccatcg agctgctgcg accggacgac acgcgcacga tgcgcgcgct caaggacgag
    45241 gtgcagctga acgtggcggt gcaatttgcc gtgcagctcg attggtcggt gacgccgatc
    45301 gacctcgaca gcgtgccgcc gcttgcgatc ttcagcgcct atacgctgta caccatcgag
    45361 tcgaaccgcg acggacgcaa gtggttcgga ctgcgcctcg gcttcttcag cgacgcgctg
    45421 tcggcgaagc aggtcgcgta ctacgtgcgt tcggacttcg cgagtgtcgc ggtggtgccg
    45481 gtgccggcgg cggaaaagga gcgcgccatc gaaaacgcgc acagccagcc gctcgtgccg
    45541 cacaagcgca gcacccggaa acccgtcgat gagttcaagc tgttcgatgc cgaaccggtg
    45601 gagaagccgc cggcgccgtc ggcacaggcc gtgcgcgcga aactcgaggc cgcggccagg
    45661 gccgacagga atccagcgcg ggcgcggccg agacccgcgg cgcgcaagaa ggcgaattcg
    45721 ctcgaggacg cactcgagca gactctggag atcctgggtg cgagcgaact cgagatcgac
    45781 aacggccgcg gcgagctgct gaacgatacc ggtgtgcgcc acctgtcggt gaagatcgac
    45841 aagcgcacct ccacgttctc gaagctgctc gaccggctct ccgatcgcgt cggcaagggc
    45901 tgactagccc ttgcggaaca agtcctcgag cgccgcgcga gagcgctcga tttcggcggt
    45961 gttcgccggc acgtacgcgc cttcacgcgg cttgaagtga tcgcagaagt tcgcgtgctc
    46021 cttgtcgcgc acttcctcgg cggtgggctc gcggcaatgc tttgcgacgc gcgtatcgta
    46081 ttccacgcat agcctgcaga catgcagctg cgcggcgcac gccggacatt cgtcgagccg
    46141 cagcagcggc agcgacagtg cggcgagcga agcgccgcat ttccagcaca cgagactgtg
    46201 ggccatcacc gaagcttagc aaggtcgcgc ctgtggcgcg cggcggctaa cattccccga
    46261 tggtgaaaca gaatgcaacg ggtgcgacag gcaccggatc gccgctgctg gtcgagttcg
    46321 cgggccgcat cgtcatgatc ggctgcggca gtatcggcca ggtgatcctg ccgctgctgc
    46381 gtaaacacat cgggttgggc cccgattcgc tcatcgtgct cgcggcggac gatacggggc
    46441 gcggcgtcgc ttacgagaat ggcgcgccgt tcttctactc gcacttgaag ccgcaccgct
    46501 tccggcggct gcttgcgcgt cacgtgcgtc agggcgacct gctgctgaac ctctcggtcg
    46561 acgtctcgag cctcgacctc gtgcgcttcg cggccgagcg cggcgcgctc tacgtggata
    46621 cctcggtcga gccctggccc ggcgttttcg acaatccgat gctcgacgtg cgccagcgca
    46681 ccaacttcat gacgcgccat cgcgcgctcg cgctcgcgcg ggaactcggc accgactcgc
    46741 cgacggcggt ggtcgaccac ggcgcgaacc cggggctcgt gtcgcacttc gtcaagcgcg
    46801 cgctgctcga cctgcagcaa gcgctgcgtg tcgacggcga gccgccggcc acccgcgacg
    46861 actgggcgac gctggcgcga gatctcggcg tgtcgctgat ccaggtttcc gagcgcgata
    46921 cccaggcgag cgatcgcccc aagcggcccg gcgagttcgt caacacctgg tcgatcgacg
    46981 gcatcctcga cgagtcggtg cagccgtcgg agatctccta cggcaccgcc gagaaacacc
    47041 ggccgcgcgg ctcgcagttt catcggcgcg attcgggcac gattttcctc gatcgcccgg
    47101 gcggcgcaac gttcgcgcgc agctggaccc catcggtcgg cggcttccag ggcctgatga
    47161 tgtcgcacga cgaagcgttc tcgatcggcg acttcctgtc gctgcgcgcc ggtgggaagt
    47221 tcacttaccg g
//
LOCUS       DAOQVY010000004        36628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_1329_length_36628_cov_5.078665, whole genome shotgun sequence.
ACCESSION   DAOQVY010000004 DAOQVY010000000
VERSION     DAOQVY010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 36628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36628
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1329_length_36628_cov_5.078665"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2035)
                     /locus_tag="PKN91_00975"
     CDS             complement(<1..2035)
                     /locus_tag="PKN91_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS26670.1"
                     /translation="MESSVNHSKYIGTAIALCLAAPLAAWAQSTDEGGLAEVTVTAQR
                     RSQSMQSVPVAVTAFDPASLDARQVLTLRDVSNVVPNLWMETNTGLSSGARAALRGVG
                     EDESFFTSDPPVGIYIDDVYIPRQTGALFDLYDVERIEVLRGPQGTLYGRNTSAGAIK
                     LVSVKPSQDTRIMAELTGGDYGRIDARASVSGGLSDTVSGQVALLSRQRDGYDRNLVN
                     GARVNDQDVVSGRAALRWQPTDALDILLSYDQTRERSTPGYATGVLLQPPGDLGPWNP
                     DEQYDGDTDVHTLRSDLLDPLNDIDQRGASLNIGWDVGNVTVRSITSWREMENTLLLD
                     ADGQDTCFGLALPCLHLFQDQTQDQFSQELQLQGTAFGDRVDYIVGAYYFTESNWQRT
                     ENIILAPFGANPYFDTSLDTDSIALFASATWAATDRLNLTAGLRWTEDSKDFRSAVFD
                     ATGAAQQVCAAPDGSQAYSAGPCTAGSPAGAVTDDLSRTVDQTWSAVTPRAAVDFQLT
                     DDAMIYASLSKGFKSGAFDGRESGLVLYTMQPIAPETTLAYEVGAKAEWFDRRLRTNI
                     ALFLNEIDDLQGTGTNLDSGTFTRFSVGDAETRGAEIEITAKPIEPLTLTAAIGLLDT
                     KYTKINFNQMADCGPVGTGDVDLELKFSPDVSSFVGASWRVPLARLRGGLEFGA"
     gene            complement(2148..3053)
                     /locus_tag="PKN91_00980"
     CDS             complement(2148..3053)
                     /locus_tag="PKN91_00980"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNS26671.1"
                     /translation="MEMRQLRHFLALMETKSYWRAAESCGITPQALSKSIRRFEEQLG
                     VRLFDRDTRSVKPTLFAEQIAGYARNIDAEARSLRRSLDTLLGAGTNQLVVGTGAAAA
                     TKIVVEAVLAVRAQNPTLAVTVLEGTFEAMVPHLMHGKVDVVVSIMTTDAVDKLIDFR
                     VLKVEQYRVYARAGHPLAGEVSVPLAKLCEFPWIAGADHDRVSEDVEASFRAAGIDPP
                     TPVFRTDSVSFAINLVMQCNALFILPSETVRREVESGQLVAIDVQAERWTRPTVVFFR
                     KNSTRSPDSIRFLKELKRIVERSTY"
     gene            2981..3919
                     /locus_tag="PKN91_00985"
     CDS             2981..3919
                     /locus_tag="PKN91_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNS26672.1"
                     /translation="MTQPRASSSSSPSAPENGAAVSFPSAARSARLKGIGYMLAAVGL
                     FAVVDVTLKVLVQWYPPLQVSFLRAATSLPVVLLPLLFNRRWGELRTSRPGFHLLRGL
                     LGVGSLLAYVLALRYLSLSNMYAIFLSAPLMIAALSIVLLKERVDWHRWFGIGAGLLG
                     VVIILRPGAGMIGLGGLAALLAALCYALNAISMRALGRTDTTVSMVWWYLVVMAIVTG
                     LLAIPGWVALELGHAAWIVALGVVGAAAQHALTEAFRCAPPSAVAPFEYTALLWGIGF
                     DLALWGVLPSSLVLAGGAVVIGGGLYVIHCERKVKS"
     gene            3916..4404
                     /locus_tag="PKN91_00990"
     CDS             3916..4404
                     /locus_tag="PKN91_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNS26673.1"
                     /translation="MSRTGPVKRVTIWVRDIERSLALYRDLLGLEVIEDKTLAGPAIA
                     GMVGYQDATMRMVHLAPPGAEYGWVGLYALIDARPPTEPVPAPREDRLSPGQAAVVLT
                     TDQIDAIARDLDAQGYRFLLRPQGYVKPTDSPKMPAGRYTEMIFFDPDGIPVSIMGYE
                     PL"
     gene            complement(4382..5143)
                     /locus_tag="PKN91_00995"
     CDS             complement(4382..5143)
                     /locus_tag="PKN91_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26674.1"
                     /translation="MSTIKRGRVLRDTSAGEGLVFVDGNQYAFRLEGVWKSEYAPKVN
                     MGVEAEFDEQGRLMALRSVSASGVAGEQAAQALGAAQESAKRLAAELQAKGLPAFQQY
                     AQRIGYPVLAAFVALIIGWFFLAAVSVDLGFMGRNSVTFWQGLKFLNSGAAAALGGGS
                     TGFYGLLCIAALLAVVLPQVWPNPRARYGMAAPLALVLLVLIVAYAKVPAVPQGAEEF
                     AAEFRKAVSLGLGTYLAFAAAIYLAWKGLRAARNP"
     gene            complement(5144..6316)
                     /locus_tag="PKN91_01000"
     CDS             complement(5144..6316)
                     /locus_tag="PKN91_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26675.1"
                     /translation="MKALLALIALAALPLAVPAADDCAGRSHCVATRSFVADVTNFRV
                     SKSGRYRVLTATVNFRNRTDKPLTLGYVGDSGIAMDEQGNRYVVANAQAVRGIGLVSG
                     SSFDPKFTLQPGEASDTRFELVWDPGKAIVGVSYELDLAIREINAVTPDQFRLGSEHS
                     VHFSQLAEGAASAAPVGANAAATGRSAPAAPAAATPAAIAADPCAGSTRCYNAGPFIA
                     EVVQLTSAAMGSGVRHHSVALNIRFRNVSDRPIILGYHSGSSAGIDNFGNTYYRGRAG
                     THDTSVKGIGYVDGRSADTQFALAPGQSRNATFNLIRYEAKPPIGNAWGYDVVIDELE
                     ILPGQQVRTVRENSLNFQNLTAGTFAGTATNPADGAAAAQDVASQVIGLFKKLKDK"
     gene            complement(6330..7517)
                     /locus_tag="PKN91_01005"
     CDS             complement(6330..7517)
                     /locus_tag="PKN91_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26676.1"
                     /translation="MISSRSILKAGVLLLFAPLALAAERDLDPEQLALTYHKLAGKAF
                     DFDRHAAASRVVGQASGFDRPDVLKQEIARQRAVFDTASDQIVFETDIQNSISDYDHD
                     RGEFSVAIFEPGSYMPVYFNRQEYRVVFANAAQARAIRMPKEEAREFDRRIMRDGNRS
                     VMTPVRFRIVGDGDPAGAVSGQYVVRAELISAQVLDGDGSVLATPDFTATPAAPPVSF
                     DPLAVDAGGLRVGVTADQLEAAVTRLYRKPVRRPRGPRSDSDPRFTGSIEIDLMECIY
                     VPSSRDREPVPGDVCLRAFYDKDGIVRQVRIQGVLPRFDYEQARSAAIARFGPATAVS
                     GGSTPQLSWGTSDPALGPGLTLRIDPYGNSLSFGSGDVRAGSLDLTLTDTAWAAATAA
                     PAN"
     gene            complement(7514..7915)
                     /locus_tag="PKN91_01010"
     CDS             complement(7514..7915)
                     /locus_tag="PKN91_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26677.1"
                     /translation="MSVQCPGLRLAPVALLLALVACGGGAPGDAANPSAASLPASRGE
                     VWMIDPASPTDAQVLAFLNGVHRFVLKDNEIYTATTRLDTAKVDGALTAATLAGDLQL
                     RIESQGVQPILVFSSGPRGPLRKYEAPEPKP"
     gene            complement(7912..8172)
                     /locus_tag="PKN91_01015"
     CDS             complement(7912..8172)
                     /locus_tag="PKN91_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26678.1"
                     /translation="MKNNLTLLIACGALAACDQGRWIELPPILMTCSDLDDEEMGAEL
                     RNPDRFSIGRSMLQGVCTQSGAGQFTGDTRCSKDVVEIRCKS"
     gene            8337..8945
                     /locus_tag="PKN91_01020"
     CDS             8337..8945
                     /locus_tag="PKN91_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNS26679.1"
                     /translation="MDKLEFIRACREGGAALERALAALDRELFTRLYRECRRAVRDSA
                     AARDLVQDTFIKVWQRCATFQGDSELWPWMRTILRRTMLDAFRRAETQVSLDDTVASR
                     EIEARIAELSVARADAPPDAAHARQLAETFQRCWTRFEQDCPAHAAVIVWISEDGLDN
                     AAVAQLLGRTPGATREYISQCRKKLRVYLADWFELAFGAPSP"
     gene            8942..9544
                     /locus_tag="PKN91_01025"
     CDS             8942..9544
                     /locus_tag="PKN91_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26680.1"
                     /translation="MTDDPTLDVWFDALAGRGTVGGAAANEARALREALRARPAIDEL
                     GLQRELATSDADRGAQLLGKARGDPILGPILARGRGRLNIARRAWGGLLAAGLAGISI
                     ALVWTLRPATEVPVYREAPDQVHRMVAADPRALRDEIARALRAAGIEVVIYERFGREG
                     LDAELPRPVTGPVRSILVLYRIPEPADGVLRIEIESGSSP"
     gene            9541..12834
                     /locus_tag="PKN91_01030"
     CDS             9541..12834
                     /locus_tag="PKN91_01030"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNS26681.1"
                     /translation="MMVRRGLAAFLLVALVIAGRSAAALELPSICTQQTPPDNASVPI
                     AELRAQVDAIGESEPMQAVAILCATIPRVAREYGDDSLEFAWWVQTLATPLIAYRDEF
                     TEAIPLLEYARPIIERELGTQSAQIAEIHVAYAWIAFRQGRIAEAGTQWDAALQIREQ
                     VPGAKQIELQKALVGLAQVRLSQRRFADARALLERAQRILTANGETTSEAAGAIENVL
                     TNIALREEDYPRARRHAEAQLAIELQLRAERGPAQPVTAWLLYGQVLEKLDEYEQAEH
                     AMREAVRLAEATDGPLQRHYLRALTGLSVLLNARGKPQEALPFARRAVEVGTQQLGAD
                     APNLVSVLLGLAEIQRVLGDLPASRALYDQAGGIIATHTADVERQVRVSYHRGLGLLL
                     QLLGATTEARAALTRGLEMAGDGAEMATERANVLLLLARLNAAGDPSLAREQLGEALA
                     LYAARLPAGHPTLLRVVNELCAIDVRTTPAVAPNCIEAQRRLEAAREVEPALRHSVYA
                     NGSALEEAHGNGGGAYSLAVRALAAAATLGTPDPEWQAQYRLARLLHQRGDHALAIFF
                     GKQSIGRVEALRAGFNDADRSLHSGFLRDKVGVYRAVADWLMESGRMEEGLQVLDLLK
                     AEELSDFVPRAGPATRPGPGIALTAAEQQLWASYLATLRADSRAGAEIDTLTRRREAG
                     RISPDERRRLDQLLASQPGEEAARARRIDAFIGGASRTAAPLPGRIISTDRLAREVER
                     FGNDSAIGVYLLTDQHLRILVATREGQAEHRIDIEATALRREIGDLLDAIGRREDVHL
                     RSRALYDILVRPLAEAAARAGAQRIVFWPDDALRYVPFAALSDGEHYLVERYAIQLYA
                     DAASRAADAPTAGREPVLAVRGLGVTQAVAGYRALPAVADELCYIVRGPITGLASPGE
                     ACDAPTPGEGPSTGRGALPGEGYADAAFTEARLDSLLAAPRDFSVLHLGTHFNLRPGN
                     AMRSYLLLGDGGQLTLDKIGALDFSGIALTTLSGCQTGMGGAVTADGREIEGLSSIVQ
                     RRGARSVIASLWRVEDHSTASLMRKMYEEMSTLRTRDGGTARALQLAQLSLLDRHPYY
                     WAGFLLTSQ"
     gene            complement(12878..14536)
                     /locus_tag="PKN91_01035"
     CDS             complement(12878..14536)
                     /locus_tag="PKN91_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNS26682.1"
                     /translation="MTTFRELGLSGPVLAALADVGYESPSPIQAATIPVLLAGSDMVG
                     QAQTGTGKTAAFALPLLSGLDLAQRKPQVLVLAPTRELAIQVAEAFQRYAAHMPGFHV
                     MPIYGGQGYGPQLHALKRGTHVIVGTPGRVMDHMKRGTLALDGLRALVLDEADEMLRM
                     GFIDDVEWILGETPATRQVALFSATMPAPIRRIAQKHLRSPVEVTIRGRTQTAANIRQ
                     RYWIVSGLHKLDALTRILEVEEFDGMLVFARTKQATVELAERLEARGFAAAALNGDVP
                     QPQRERIVAQLKSGELDIVVATDVAARGLDVERIGHVVNFDVPTDPESYVHRIGRTGR
                     AGRSGEAILFIAPRERNLLRAIERATRQTIEPMELPSVEDVNLQRVARFKQRIKAALE
                     SGAGSAFHAMVAEVAREQGVTDLDVAAALAAIVQGETPLLLPAEPKPRTPPPPREPRS
                     RREPERRPRGEAERRPRREAGRAVDLEMETYRVEVGRAHGVRPANIVGAIASEAGLAG
                     KHIGRVDIRGEHSFVDLPAGMPKEILRELRKVRVMGQALKIRKA"
     gene            complement(14533..15108)
                     /locus_tag="PKN91_01040"
     CDS             complement(14533..15108)
                     /locus_tag="PKN91_01040"
                     /inference="COORDINATES: protein motif:HMM:NF018949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HNS26683.1"
                     /translation="MDMMWLVIGVIVFFGTHSVAIFSPTGREYLATRYGLGWRAFYSI
                     LSLVGLALIIWGYGLARQEPLVLYQPPAWLRSVVALLMLPVFPLLFAAYLPGRIKAKL
                     KHPMLAAVKLWALAHLLANGTLADVVLFGAFLAWAVVDRISFKHRVQRVIPSAPPGRW
                     NDLIAVVLGLGAYLLFVLVLHARWIGVPIAP"
     gene            15101..15742
                     /locus_tag="PKN91_01045"
     CDS             15101..15742
                     /locus_tag="PKN91_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HNS26684.1"
                     /translation="MSIPSPADAHPTPESAACERNKQPILDLLRTAFARQRRVLEIGS
                     GTGQHAVFFAREMPHLEWLPSDRAEWLPGLRARIAQHGPPNLAPPVELDVTMPAWPVT
                     GVDAVFSANTLHIMGPREVEAFFAGVSRLFAAGAAAAPPPHTLAVYGPFRYRGAFTSA
                     SNAEFDAFLRARDPASGIRDFEVVNELAAAQGLALCADHAMPANNQLLVWCNS"
     gene            complement(15715..16473)
                     /locus_tag="PKN91_01050"
     CDS             complement(15715..16473)
                     /locus_tag="PKN91_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNS26685.1"
                     /translation="MEQLLDLATQPAAWAALATLIAMEVVLGIDNLIFISILTNKLPE
                     HQRASARRIGIGVALVLRLLLLGTIALIVRLTEPVIELFGNGFSWRDIILIAGGLFLV
                     WKATTEIHQSVDPDAAPHVLVGKQATLGFASAIAQILMLDLVFSLDSIITAVGMTEHI
                     PIMVIAVIVAVIAMLLAAEPLANFIHANPTIVMLALGFLLMIGTTLIADGFGVHVPRG
                     YVYAAMAFSAFVEGLNMAARRAKRRRQELHQTSN"
     gene            complement(16487..17371)
                     /locus_tag="PKN91_01055"
     CDS             complement(16487..17371)
                     /locus_tag="PKN91_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26686.1"
                     /translation="MRVIAACAIALLLALIVYPPALPSTAWMGGVHDAGHVLVFAVIT
                     ALLCVILRPRGARLAVIAAMIVLLAAGTELAQGRFGVGDATFGDFGRDLLGGAVGVTL
                     WFAATRRLPALHLVAALALLAGLLPLARIGWAYAQRADAPHVIWDPRRATWDVFIESP
                     RTGTFSKLPDGQGVRFVAGTDSYAGIVIREPPPDWRGFDALIVSVANPGSRALSLNVR
                     IDDLPRDTDYEDRFNRERVLPPGAALRWRIPLAEIEQGPRGRPLGLSHITRVVIFLSP
                     GYRGAEFDLRDVRLEPAP"
     gene            17402..17860
                     /gene="nikR"
                     /locus_tag="PKN91_01060"
     CDS             17402..17860
                     /gene="nikR"
                     /locus_tag="PKN91_01060"
                     /inference="COORDINATES: protein motif:HMM:NF002815.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HNS26687.1"
                     /translation="MQRVTISLDDELAASFDRLIAGRGYENRSEAVRDLIRQQLDREA
                     QDHAPHGHCVASLSYVYNHHARDLAERIAERQHAHHDLVVATMHVHLDHENCLETAVL
                     QGPVAAVRELAGSLTAEPGVRHGSLNLVAVEQAEHHAHGGRTHRHMKPRY"
     gene            17911..20781
                     /locus_tag="PKN91_01065"
     CDS             17911..20781
                     /locus_tag="PKN91_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS26688.1"
                     /translation="MESLPASFGGLAVVAFLLGLKHGFDADHLATIDGLTRVNTGAGR
                     PFARYCGALFSLGHGIVVIAIALLVSTAVAHWQTPDWLQTTGVAISIFFLTLLGILNL
                     HAVWRAAPDEVVTPVGIKGRFLGALGRAKNPFIVALVGALFALSFDTISQAAFFALAA
                     GQYGGAIYAVSLGVLFVLGMLVTDGVNGLWISRLILRADETARIASRVMSLAVACVSL
                     LVAGFSATKLLLPRIDSWAEGKELVFGAVVVAVVLAGFLWGRSVAGARAARMPRGGGA
                     VAAVVAVALTALIGVEARAGGITGAGLEEVTVRANRLPLTGRPRSASEGTVLGEQLEY
                     RPLLRVGELLETVPGLIVTQHTGDGKANQYFLRGFNLDHGTDFTTRVEGMPVNMPTHG
                     HGQGYMDANFVIPELVDRIVYRKGTYYADLGNFSAVGAAELHYVDAVRPFVAASAGED
                     GYLRALAGGSMPLAGGSLLAGFEYDRTDGPWVLPEDLTKVNGVLRWSRSSETSGLALD
                     LMASDGEWTSTDQIPRRAVDSGALDRYGYVDPTAGGESHRYSLSASGFRSYGPGRLDF
                     SAYAMDYRLQLYSNFTYALDPVNGDQFEQFDDRRVYGGALTWSQPFAFGAGGSVWRAG
                     ADLRVDDISPVGLYLTTARARHETIREDRVEQTLASAWTAVATQWTPWLRSELGLRAD
                     RLGYDVASDLAANSGTGHDSLVSPKSSLALGPWRDTEFFVAAGRGFHSNDVRGATIAV
                     DPTDGVTPVDRVTPLARADGSEIGLRTALLPRTQLSVALWRLDLDSELLFIGDGGTTE
                     ATRPSRREGVELSVYSRPRDWLIVDADYAASKARFRDADPAGERIPGAVEQVASVGVT
                     LQTASGWFGGARLRYLGPAALLEDNSVRSSSSTLVNLEAGRRLGEHWRVSLQLLNAFD
                     RQVNDITYYYESRLPGEAAPVEDLHFHPAEPRTLRATMHYSF"
     gene            complement(20939..22312)
                     /locus_tag="PKN91_01070"
     CDS             complement(20939..22312)
                     /locus_tag="PKN91_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HNS26689.1"
                     /translation="MNLNAFKAYDVRGRIPDEINTTLARDIGRAYAAFVQPKKVIVGR
                     DIRHSSDELVQALSEGLLESGVDVHDIGLCGTEGVYYATFAESMDGGIMVTASHNPPD
                     YNGMKFVREGSRPISGDTGLKDMRAMIEAGRLPPPAAKRGTRHVLATGARYLEHILGY
                     IDRAKLRKLKVVVNAGNGGAGLIVDQLEPHLPYDFVKLNNAPDGSFPNGVPNPMIEAN
                     RASTIEAIKRSGADVGIAWDGDYDRCFFFDEQGTFIEGYYLVGLLAEVFLKREKGGRI
                     VHDPRLTWNTIDVVERNGGKAVLSKSGHAFIKQVMREVDGIYGGEMSAHHYFRDFSYC
                     DSGMIPWLIVLQVMCETGKSLSQLVGERMRLFPASGEINRRVPDGPATIAAIQAKYQP
                     QALSADFTDGLSLEFDRWRMNLRTSNTEPLIRLNVESRADVALMQAKTAEVLGEMAKL
                     GAVAADH"
     gene            22323..22916
                     /locus_tag="PKN91_01075"
     CDS             22323..22916
                     /locus_tag="PKN91_01075"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNS26690.1"
                     /translation="MASKPRRILILDGHPDGGGRRFVHALAEAYAQGAREAGHDVRTL
                     AIGQLDIPLLRKGEDFQAGTPSREVRRWQEAIGWAGHLVVLYPLWLGDMPAVLKAFFE
                     QVLRPGFAFAASGETRLPKKLLTGRSARVIVTMGMPALFYRWYYRAHSLKNLERNILA
                     FSGFAPIRSTVVGTVDGKAASRTAWLEKVRHLGRRAV"
     gene            complement(22964..24064)
                     /gene="wecB"
                     /locus_tag="PKN91_01080"
     CDS             complement(22964..24064)
                     /gene="wecB"
                     /locus_tag="PKN91_01080"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNS26691.1"
                     /translation="MKNIHLIAAARPNFMKIAPLYHALSKESWCRPSIVHTGQHYDPN
                     MSDAFFRDLAMPKPHFHLEIGSGTHAEQTGGVMIAYEKVAMAEKPDWIVVVGDVNSTA
                     ACAMVGAKLWIPVVHLEAGLRSRDRRMPEEINRLVTDAIADVLWTPSRDGDENLLREG
                     VPAERIECIGNIMIDSYEMLRGAIEADGTRRDLGLAPQGYGIVTLHRPSNVDTREALG
                     PLVESLLEVSRRVPLVFAVHPRTRKKLQEFGLLDRLAGEGAVRVVDPLGYIQFMNLVT
                     GARLVITDSGGVQEETTYLGIPCLTLRENTERPVTVTEGSNRLVQPAALVAGVDSVLA
                     HVGRLGHVPELWDGQAASRAVASLKRRSGVGA"
     gene            complement(24061..25467)
                     /locus_tag="PKN91_01085"
     CDS             complement(24061..25467)
                     /locus_tag="PKN91_01085"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HNS26692.1"
                     /translation="MLIPVILSGGAGTRLWPLSRELYPKQLLPLVGEQTMLQATAMRL
                     TGTGAVGPIVVCNESHRFMVAEQLRQIGAEPRAIILEPAGRNTAPAIALAAQVALDNA
                     PGADPILLVLPADHVIRDVPAFQAAVRAAVAAAGLGHLVTFGVVPTAPETGYGYIQRG
                     APVAGAARIAAFVEKPALAVAEQFVNSGNYRWNSGMFVFRASRYLEELRKHAPDIAEA
                     CAAALASARRDLDFTRIDAKAFAACRSDSIDYAVMEKTSDAVVVSLDAGWSDVGSWSA
                     LYDALDRDEAGNVCRGDVIVEDSSGNYLYAESRLVAAVGLRDHVVVETKDAVLVAPKS
                     RVQDVKKVVERLKKDGRYEHSLHREVYRPWGSYDSIENGERYQVKRLSVNPGAQMSLQ
                     MHHHRAEHWIVVSGTARITRGEETFLLEENQSTYIPIGTKHRIENPGKIMLHMIEVQS
                     GSYLGEDDIVRFEDIYGR"
     gene            25607..25855
                     /locus_tag="PKN91_01090"
     CDS             25607..25855
                     /locus_tag="PKN91_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26693.1"
                     /translation="MAVLTIRNVDETVKTRLRMRAARHGRSMEDEVRQILKAAVEEKP
                     NSSPGFAASVREAFAALGGVDLDIPPREPPREPPRLDD"
     gene            25860..26285
                     /locus_tag="PKN91_01095"
     CDS             25860..26285
                     /locus_tag="PKN91_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNS26694.1"
                     /translation="MIVVDTNVLSELARHAPDAHVLHWFEAQPDGALYTTAITHAEIL
                     FGLALLPRGVKRDLVEAAITRVFTGTYADRILPFDSAAAPEYARICAARRAAGRPISQ
                     IDAQIAAITRARRARLATRDVAGFKGLDIEIVNPFGTTA"
     gene            complement(26309..27637)
                     /locus_tag="PKN91_01100"
     CDS             complement(26309..27637)
                     /locus_tag="PKN91_01100"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNS26695.1"
                     /translation="MRVTIFGSGYVGLVTGACLADAGNDVLCVDVDPLKIERLSRGEV
                     PIHEPGLDTLIKKNAEAGRLRFTTDAAEGVKHGLFQLIAVGTPPDEDGSADLKYVLAV
                     AHTIGAHLEEYRVVIDKSTVPVGTADKVKAEIAATLKERGADVEFDVVSNPEFLKEGA
                     AVGDFLKPDRVIVGTDNPRATELLRALYEPFTRSRDRLIVMDLRSAELTKYAANAMLA
                     TKISFMNELANIAERVGADIEKVRVGIGSDPRIGYSFIYPGTGYGGSCLPKDVQALIR
                     SSNAAGYQPALLEAVESVNGRQKAFLVGKIDKHFGGDLKGRTFALWGLAFKPNTDDMR
                     EAPSRTIMEMLWAAGARVRAYDPVAADEARRVFGAHEDLAICASAYEAAEGADALVIA
                     TEWQEFRSPDFDRLKAGLKQPAIFDGRNLYDPQLLQRFGFSYYAVGRGQG"
     gene            complement(27708..28805)
                     /locus_tag="PKN91_01105"
     CDS             complement(27708..28805)
                     /locus_tag="PKN91_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26696.1"
                     /translation="MVSLALGSLASFLVCTISVLALRPIAVAIDLVDKPGGRKTHHGE
                     VPVVGGLAMFLGLSVGVGLGPASLLRADTFIAACGLLVVTGLLDDRFQLSPWLRLPVQ
                     AAATLLAATHLFPGSGFSLGNPLGYGDIEFHGLLTYIAILFFVVGAINAFNMLDGMDG
                     LAGATALVSLVGISLIAWRGGLPFTLQVGLTLAGAVGAFLLFNLPIRRNRGVRCFMGD
                     AGSTLIGFVLALLCVRLTQGSIRAMAPVSVLWLVALPVFELLWTIIRRVSRRQSPFSA
                     DREHFHHLLVDSGLGVRGAFIVYVTCAMALAAAGLVFHEVGMPESLQFALFVLTGVFT
                     VMLTNRAPVLIAHLPAAWHRSATGQVHSDAG"
     gene            29025..30761
                     /locus_tag="PKN91_01110"
     CDS             29025..30761
                     /locus_tag="PKN91_01110"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNS26697.1"
                     /translation="MTSTQMPLPARLRPLSPLLLTAVLLAGCGGDSSNSPPTAYPQAL
                     TIDEDATTEIALSASDREGDVLTYAIASFPTNGTVQLDASSGRVLYVPDADYAGTDRF
                     TFTAHDGRNMSTEATVAITLRQINDAPRFPDIPELTNLPDSPITQWQLAVVDPESTPV
                     QVSLAVADPSMLEATYNTTNDTLELRPLQYGDTQVTVSASDGELSTARTFVFRVGKVT
                     RKVELAIPDPSRQAIVITNTLQYGINVGLGWNRKIIFESRADLVNAAVSPLESDENVI
                     ADALWRFMIGHVYHNESFAAERWEHDPLLLINSIGFGLCDDVSSALAFLARERGLAAR
                     VWALGGHVAPEINVAGRWQLYDPDIKVVYHDYDARITNVETLSAFPALITSPVQPLYG
                     ASFYGYQSFIADIYASHDDNNVEPWYDEAAPAVPGTFQFPPGASVHFPGSADITVNDH
                     MGMSAPAVSSLDLVLPAGTRAELKMPLVLLDIKGEGTIQIGGHDYVLGSSALDELFAS
                     NRAFIPGITIVDAKTPLVLTFLLNPRNADLETSTSVYLTGLEVWGLDVTTENFPDGRP
                     SFSAPQIPTFPF"
     gene            complement(30826..32547)
                     /locus_tag="PKN91_01115"
     CDS             complement(30826..32547)
                     /locus_tag="PKN91_01115"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNS26698.1"
                     /translation="MLALSAASLFGCGGGAQGRGSLVVESHAVSILEETRLLGQLSAT
                     GAATGAVSFTVTTAPLHGSLTLDAATGSFVYDPLEDFFGVDAFGVIASSGGISSEQAT
                     VSIHVQNVNDTPRLREIGTVLNSPEAQDVVLHSLVTDPDGDAVELTVELADSRVADAW
                     IDEDTGALVLRALAVGETGGIVTASDGQSASSMEFRYDAREVVKRREYSIPDPTTSAM
                     EIRNESASKVAFHLTYSGVPVFESVQDMLHFVRALPDEFPGESLPRKIWRFVRDFSYH
                     NAPFSSRNWLYSPWVTINSLGWGFCAHVSAVFVEIARAAGYDARVWGLDGHVVAEVRV
                     DGMWQMYDADLAVYYRGRSGRVAGVEELANDPDLIVNPIAPIFEGSSYDFPYSPTVAE
                     IYASVDDNFVGDSIFLAQDPMPGSRFELPPGSRLVFPGRWTEPPTAYDGSEPRLITQY
                     GQVALEIPPGWTGSLSLPLMLWEIQGSGTVAIDGTEFTVGSERLRDRIQNTDAPITSV
                     LILANDGLRLVHFVNAMQFAMRSSEVVDVKGLDVWALETSVIGVPANEARGSVRMRKP
                     LPTVIDY"
     gene            complement(33152..34261)
                     /locus_tag="PKN91_01120"
     CDS             complement(33152..34261)
                     /locus_tag="PKN91_01120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS26699.1"
                     /translation="MGGAESVLLRLLESSPWRSDSVVISLGRDGVLAPKFRDLGVPVH
                     ELGMNAVLPNPFAVASVARILKAHGADVVSTWMYHADLVGGIAARLAGLPVIWGIRNS
                     TLSRTDSAWTTRAIARLNGILSRWLPAVIVSCSERARDVHIELGYVRDKFTIIHNGID
                     IRRFQPDAHARAAVRGELNLSGENRLIGLIARFDPQKNHRGFIAAVRNLLERWPQLHV
                     VMAGAGITSDNKVLCAWIAEAGIADRVHLLGARWDINRVMAALDCSVLPSFGEAFPSV
                     LAESMACGVPCVTTDVGDAAAIVGTSGIVVPIGDMTALVKGIDELLEEDAETQRRRAT
                     LARARVAEMFDISTMAAAFEQLLRDTSAKYSRIRE"
     gene            complement(34338..35468)
                     /locus_tag="PKN91_01125"
     CDS             complement(34338..35468)
                     /locus_tag="PKN91_01125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNS26700.1"
                     /translation="MTTRRKVFIAVSSMEGGGAERVASTLANGWADLGWNVTLVVSFS
                     GRGKCSYVLAPAVRLVYLADMVPARCGRFVSRLARLWALRRLLRTAPPDNLVSFLPHV
                     NVASVIASRGTGVPVVVSERTNPALDREATSMQRVLRRWLYRFADAVVIQTEGVRESV
                     MRCAPGIRRIVVIPNPVPSSLAGTKAEVESGGRRRLIACGRLVPEKQFGGLLDTFANL
                     KQEFNQWDLWIWGEGPLRQELEHQVNARGLAGRVFLPGHTDSLWEEMQRADLFVLTSS
                     VEGFPNALLEAMAAGIPAVSFDCDFGPREITCDGTHGALARLNDWPGLERALRALMDD
                     PLRRKQLGENGSRAVRERYSQVRVIGLWCDLLEELAPREVPS"
     gene            complement(35465..>36628)
                     /locus_tag="PKN91_01130"
     CDS             complement(35465..>36628)
                     /locus_tag="PKN91_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNS26701.1"
                     /translation="GCTDVSVLAGNAVTGPKLLILVTEDWYFHSHRLALARTAVTSGF
                     EVVVATRIGELRPAIEAAGIRVIDYPFDRRGMNPLRAILDVLRLARLIRSERPDIVHN
                     VALKPVVLGGLACRLSGCRNVVNAIAGMGFLFTERSRAPLVSAIVRILLAMVGSRGVT
                     IVQNPDDGRLLAKAGVPASRIRHLPGVGVDLQAFQQSPEPSGVPVAALPARLLWDKGV
                     AEFVEAARILKSRGVAVRCVLVGMPDKGNPTSVGTEQVAAWVKEGVVEWWGHSTDMPA
                     TFSRTSIVCLPSYREGFPKVLAEAAACGRPIVTTDVPGCREVVTDGDNGMLVPPRSAG
                     QLAMAIEALAADRALRHQFGLAGRRRAEREFSSESANASMLRIYGEMLTGLQA"
BASE COUNT         6367 a        12518 c        11664 g         6079 t
ORIGIN      
        1 cggcgccgaa ctcgaggccg ccgcgcaggc gtgccagcgg cacgcgccac gatgcgccga
       61 cgaacgagga cacgtcaggc gagaacttga gctcgaggtc cacgtcaccg gtgccgacgg
      121 ggccgcagtc ggccatctgg ttgaagttga tcttcgtgta cttggtgtcg agcaggccga
      181 tggccgcggt gagggtcagc ggctcaatcg gcttcgccgt gatctcgatc tccgcgccgc
      241 gcgtctcggc atcgccgacc gagaagcgcg tgaaggtgcc gctgtcgagg ttggtgccgg
      301 tgccctgcag gtcatcgatc tcgttgagga acagcgcgat gttggtgcgc aggcgccgat
      361 cgaaccattc ggccttcgcg cccacctcgt acgccagggt cgtctccggc gcgatcggct
      421 gcatcgtgta cagcacgagg ccggactcgc ggccgtcgaa cgctcccgac ttgaagccct
      481 tgctgagcga cgcatagatc atcgcgtcgt cagtgagctg gaagtcaacc gcggcgcgcg
      541 gggtcaccgc cgaccaggtc tggtcgacgg tgcgcgacag gtcgtctgtc accgcaccgg
      601 cgggtgatcc tgccgtgcac ggccccgccg aataggcctg cgagccatcg ggcgccgcgc
      661 agacctgctg tgcggcgccg gtggcgtcga acacggccga tctgaagtcc ttcgagtcct
      721 ccgtccagcg caggccggcg gtcagattga gccggtcggt cgccgcccag gtcgccgaag
      781 cgaacagcgc gatgctgtcg gtatcgagcg aggtatcgaa gtaagggttg gcgccgaacg
      841 gcgcgagaat gatgttctcg gtgcgctgcc agttcgactc ggtgaagtaa taggcaccga
      901 cgatgtagtc cacgcgatcg ccgaacgccg taccctgcag ctgcagctcc tggctgaact
      961 gatcctgggt ctggtcctgg aacaggtgca ggcagggcag cgcgagcccg aagcaggtgt
     1021 cctgaccgtc ggcgtcgagc agcagcgtgt tctccatctc gcgccaggaa gtgatcgagc
     1081 gcacggtcac attgccgaca tcccaaccga tgttgaggct cgcgccgcgc tggtcgatgt
     1141 cgttcagcgg gtcgagcagg tcggagcgca gtgtgtgcac gtccgtgtcg ccgtcgtact
     1201 gctcatccgg gttccatggc ccgaggtcgc cgggcggctg cagcagcacg ccggtggcgt
     1261 agcccggggt agaacgctcg cgcgtctggt catacgagag caggatatcg agcgcgtcgg
     1321 tcggctgcca gcgcagcgca gcgcgacccg aaaccacgtc ctggtcgttg acgcgcgcgc
     1381 cgttgacgag attgcggtcg tagccgtcac gctggcgcga cagcagggcg acctggccgg
     1441 acacggtatc cgagaggccg cccgaaacgc tcgcacgagc atcgatacga ccgtagtcgc
     1501 cgccggtcag ctcggccatg atgcgcgtgt cctgcgaggg cttcacgctg accagcttga
     1561 tcgcgccggc cgaggtgttg cgcccgtaca gcgtgccctg cgggccgcgc agaacctcga
     1621 tgcgctcgac gtcgtagaga tcgaacagcg caccggtctg gcgcgggatg tagacgtcgt
     1681 cgatgtagat gccgaccggc ggatccgagg tgaagaagct ctcgtcttcg cccacgccgc
     1741 gcaacgcagc gcgcgcgccg ctggaaaggc cggtattggt ttccatccag agattcggca
     1801 cgacgttgga aacgtcacgc agcgtcagca cctggcgtgc atcgagcgac gccggatcga
     1861 acgcggtgac cgcgacgggc acgctctgca tgctctggct gcgccgctgc gcggtcacgg
     1921 tgacctcggc gagaccgccc tcgtccgtcg actgcgccca cgccgcgagc ggcgcggcaa
     1981 gacacaatgc gatggccgtg ccgatatact tcgaatggtt gaccgatgat tccatacgcc
     2041 cctcccgcag gattccccaa ggttccgtca ctcgtccgag tatcggcagc aggccgggca
     2101 ggaggccatc aacttgcggt ttttataatc aacccgaagt tgttgcgtca gtaggtgctg
     2161 cgctccacga tgcgcttcag ttccttcagg aaccggatcg aatccggcga gcgggtgctg
     2221 ttcttgcgga agaacaccac ggtcgggcgc gtccaccgtt cggcctgtac gtcgatcgcg
     2281 acgagctggc cggattccac ttcacgacgc accgtttcgg acggcaggat gaacaacgca
     2341 ttgcactgca tgacgagatt gatggcgaac gacaccgaat cggtgcggaa caccggcgtc
     2401 ggcggatcga ttccggccgc gcgaaagctc gcctccacgt cctcggacac gcgatcgtgg
     2461 tcggcgcccg cgatccacgg gaactcgcac agcttcgcga gcggcacact cacctcgccg
     2521 gcgagcggat gaccggcgcg ggcgtatacg cggtattgct cgaccttcag gacgcgaaag
     2581 tcgatcagct tgtcgaccgc gtcggtggtc atgatgctga caaccacgtc gaccttgccg
     2641 tgcatcaggt gcggcaccat ggcctcgaat gtcccctcga gcacggtgac cgccagggtc
     2701 gggttctgcg cgcgcaccgc gagcaccgcc tcgacgacga tcttggtggc cgccgccgcc
     2761 cccgtgccga ccaccagctg gttggtgccg gcaccgagca gcgtgtcgag cgagcggcgc
     2821 aggctgcgcg cctcggcatc gatgttgcgg gcatagcctg cgatctgctc ggcgaacagc
     2881 gtgggcttca ccgagcgcgt gtcgcgatcg aacaggcgca cgccgagctg ctcctcgaag
     2941 cgccggatgc tcttgctgag cgcctgcggc gtgatgccgc atgactcagc cgcgcgccag
     3001 tagctcttcg tctccatcag cgccagaaaa tggcgcagct gtctcatttc catcggctgc
     3061 caggtccgct cggctcaagg gcatcggcta catgctggca gccgtcgggc tgttcgcggt
     3121 ggtcgacgtc acgctgaagg tgctcgtgca gtggtacccg ccgctgcagg tgagcttcct
     3181 gagagcggcc acgtcgctgc cagtcgtact gctgccgctg ctgttcaatc ggcgctgggg
     3241 cgagcttcgc acgtcgcggc ccggatttca cctgctgcgc ggactgctcg gcgtcggctc
     3301 gctgctcgcc tacgtgctgg cattgcgcta cctcagcctg tccaacatgt acgcgatctt
     3361 cctgtccgcg ccgctgatga tcgccgcgct gtccatcgtg ctgctgaagg aacgcgtcga
     3421 ctggcaccgc tggttcggca tcggcgcggg tctcctcggt gtggtgatca tcctgcgacc
     3481 gggcgcgggc atgatcggcc tgggcggtct cgccgccctg ctcgcggcct tgtgctatgc
     3541 gctgaacgcg atctcgatgc gcgcactcgg acgcaccgac accacggtgt cgatggtatg
     3601 gtggtacctg gtggtgatgg cgatcgtcac cggcttgctg gcgatacccg gctgggtcgc
     3661 gctcgagctc gggcatgcgg cctggatcgt cgcactcggc gtcgtcggcg cggccgcaca
     3721 gcatgcgctc accgaagctt tccgctgcgc gccgccctcg gcggtcgcgc ctttcgagta
     3781 cacggcgctg ctgtggggca tcggcttcga cttggcgctg tggggcgtgc tgccctcgtc
     3841 gctcgtgctc gccggcggcg cggtcgtcat cggcggcggg ctgtatgtca tccactgcga
     3901 acgaaaggtg aaatcatgag ccgtaccgga cctgtcaagc gcgtcacgat ctgggtgcgg
     3961 gacatcgagc gctcgctcgc gctttaccgc gacttgctcg gcctcgaggt catcgaggac
     4021 aagacactcg ccggcccggc gatcgccggc atggtcggct accaggatgc gacgatgcgc
     4081 atggtgcacc tcgcgccgcc gggcgccgag tacggctggg tgggcctcta cgcactgatc
     4141 gacgccagac cgccgaccga acccgtgccg gcgccgcgcg aggatcgcct gtcgcccggc
     4201 caggcggcgg tcgtgctgac gaccgaccag atcgatgcga tcgcgcgcga tctcgacgcg
     4261 caaggatacc ggttcctgct acgcccgcag ggctacgtga agcccaccga cagcccgaag
     4321 atgcccgccg gccgctacac cgagatgatc ttcttcgacc ccgacggcat accggtcagc
     4381 atcatgggtt acgagccgct ctgaggccct tccacgcgag ataaatcgcc gccgcgaatg
     4441 cgaggtaagt cccgaggcca agcgacaccg ccttgcggaa ctccgcggcg aattcctcgg
     4501 cgccctgcgg cactgcgggg actttcgcgt acgcgacgat cagcaccagc agcaccagcg
     4561 caaggggcgc cgccatgccg tagcgggcgc gcggattcgg ccagacctgc ggcagcacca
     4621 cggcgagcag cgccgcaata cacagcaagc cgtaaaagcc ggtgctgccg ccgcccagcg
     4681 ccgccgccgc gccgctgttc aggaacttga gcccctgcca gaaggtcacc gagttcctgc
     4741 ccatgaagcc gaggtcaacc gacacggcgg cgaggaagaa ccagccgatg atcagcgcca
     4801 cgaaggccgc cagcaccggg tagccgatac gctgtgcgta ctgctggaac gccggcaggc
     4861 ctttcgcctg cagttcggcc gccaggcgct tcgccgattc ctgcgcggcg ccgagcgcct
     4921 gcgcggcctg ctcgccagcg acgcccgatg cgctcacgct gcgcagtgcc atgagccgcc
     4981 cctgctcgtc gaattccgcc tcgaccccca tgttcacttt gggcgcgtac tccgacttcc
     5041 acacgccctc gagtcgaaag gcgtactggt taccgtcgac gaacacgagt ccctcaccgg
     5101 cgctcgtgtc gcggagcacc cgaccccgtt tgatcgtgct cattcacttg tccttgagtt
     5161 tcttgaacag accgatgacc tggctcgcga catcctgcgc cgcggcagcc ccgtcggccg
     5221 ggttcgtggc cgtgccggcg aacgtgcccg ccgtgaggtt ctggaagttg agactgttct
     5281 ccctgaccgt gcgcacctgc tggccgggca ggatctcgag ttcgtcgatc acgacgtcat
     5341 agccccaggc gttgccgatc ggcggcttcg cctcgtagcg gatcaggttg aaggtcgcgt
     5401 tgcggctctg gccgggtgcc agcgcgaact gcgtgtcggc actgcgtccg tccacatagc
     5461 cgatgccctt gacgctggtg tcgtgcgtgc cggcccggcc gcggtagtag gtgttgccga
     5521 agttgtcgat gcccgcactc gaaccggagt ggtagccgag gatgatcggc cggtcgctga
     5581 cattgcggaa gcgaatgttg agcgcgacgg aatgatgccg cacgccgctg cccatcgcgg
     5641 cggaggtgag ttggacgact tcggcgatga acgggcccgc gttgtaacag cgtgtggatc
     5701 ccgcgcacgg gtcggcggcg atcgcagccg gcgtggcggc tgccggcgcc gctggtgccg
     5761 acctacccgt ggccgccgca tttgcgccca ccggggccgc gctcgctgcc ccttcggcga
     5821 gctgcgaaaa atgcaccgag tgctcggagc cgagccggaa ctggtccggg gtcaccgcgt
     5881 tgatttcgcg gatcgccaga tcgagctcgt agctcacgcc gacgatcgct ttgcccgggt
     5941 cccagaccag ctcgaagcgc gtatcgctcg cctcgcccgg ctgcagggtg aacttgggat
     6001 cgaagctgct gccggacaca aggccgatgc cgcgcacggc ctgcgcgttc gccacgacat
     6061 agcgattgcc ctgctcgtcc atcgcgattc cggagtcccc cacgtaaccg agcgtgagcg
     6121 gcttgtcggt ccggttgcgg aagttcacgg ttgcggtcag cacgcggtag cgcccgctct
     6181 tgctgacccg gaagttcgtg acgtccgcga cgaagctgcg cgtcgcgaca cagtggctgc
     6241 ggccggcaca gtcgtccgcc gctggtaccg ccagcggcag cgcagcgagc gcgataaggg
     6301 cgagcaaggc tttcatgtcg atggctcctt cagtttgcgg gcgcggcggt ggccgccgcc
     6361 cacgccgtgt cggtcagtgt cagatcgagt gaaccggcgc gcacgtcgcc cgagccaaag
     6421 ctgagcgagt tgccgtacgg gtcgatcctg agcgtaaggc cggggccgag agccggatcg
     6481 ctcgtacccc agctgagttg cggcgtgctg cccccgctca ccgccgtcgc gggaccgaag
     6541 cgcgcgatcg cagcactgcg cgcctgctcg tagtcgaagc gcggcagcac gccctggata
     6601 cgcacctggc gcacgatgcc gtccttgtcg tagaaggcgc gcaggcagac atcgcccggc
     6661 accggctcgc ggtctcggct ggacggcaca tagatgcatt ccatcaggtc gatctcgatc
     6721 gagccggtga agcgcggatc gctgtccgag cgcggcccgc gcggcctgcg aaccggcttg
     6781 cggtacaggc gtgttaccgc cgcttccagc tggtcggcgg tgacgccgac gcgcaatccc
     6841 ccggcgtcga cggcgagcgg atcgaacgag acgggcggcg cggccggggt tgccgtgaag
     6901 tcgggtgtcg cgagcacgct gccgtcgccg tcgagcacct gcgccgaaat cagttcggcg
     6961 cgcaccacgt actgaccgct cacggcgccg gctggatcgc cgtcaccgac gatgcggaac
     7021 ctgaccggcg tcatgaccga gcgattgcca tcgcgcatga ttcgccggtc gaactcgcgc
     7081 gcctcctcct tcggcatgcg gatcgcgcgt gcctgcgccg cgttcgcgaa caccacgcgg
     7141 tactcctgcc gattgaagta gacaggcatg tacgaaccgg gctcgaagat cgcgaccgag
     7201 aactcgccgc ggtcgtggtc gtagtcgctg atcgagttct ggatgtcggt ctcgaacacg
     7261 atctggtcgc tcgcggtgtc gaacaccgcg cgctgacgcg cgatctcctg cttcagtacg
     7321 tcgggccggt cgaagcccga ggcctgtccg acgacgcggg acgcggccgc atgacgatcg
     7381 aagtcgaatg ccttgcccgc gagcttgtga taggtgagcg cgagctgctc gggatcgaga
     7441 tcccgctctg cggcgagagc gagcggcgcg aacagcagga gcacgccggc tttcagaatt
     7501 gaacggctgc tgatcatggc ttcggctccg gtgcttcata cttgcgcagc ggcccgcgcg
     7561 ggccactgga gaacacgagg attggctgca caccctgcga ctcgatgcgc agctgcagat
     7621 cgccggccag ggtcgccgcg gtcagtgcgc catcgacctt cgccgtgtcg aggcgcgtgg
     7681 tcgccgtgta gatctcgttg tccttcagca cgaagcggtg cacgccattc aggaacgcga
     7741 gcacctgcgc atcggtcggc gaggccggat cgatcatcca aacctcaccg cgcgacgccg
     7801 gcaggctcgc ggccgagggg ttggcggcat cgcccggtgc gccaccgccg caggccacca
     7861 gtgcgagcag cagcgccacc ggcgccaggc gcaggcccgg gcactgcacg ctcatgactt
     7921 gcaccggatt tctacgacat ccttgctgca gcgggtgtcg ccggtgaact ggcctgctcc
     7981 gctctgtgtg cacacaccct gcagcatgct gcgaccgatg ctgaacctgt ccgggttcct
     8041 gagctcggcg cccatctcct cgtcgtcgag gtcgctgcag gtcatcagga tcggcggcag
     8101 ttcgatccac cgcccctggt cgcaggccgc gagcgcgccg catgcgatca gcagagtcag
     8161 gttgttcttc atgggctggc cccctgtgcc atggcttgcc ggacaggggt gagacgcagg
     8221 cccctcgcat ctgtcaggct accgcccggt aatcctgacg aacgcgcacc atgttgcgtc
     8281 cttaccggca tgcggatatt tcacgccgac tccggtaggc tgtaccgggg gatagcttgg
     8341 acaagttgga gttcatccgg gcttgccgcg aaggcggcgc tgcgctcgag cgggcgctgg
     8401 ccgcgctcga ccgcgagctg ttcaccaggc tctaccgcga atgccgccgc gcagtgcgcg
     8461 actccgcagc cgcacgcgac ctcgtccagg acaccttcat caaggtgtgg cagcgctgcg
     8521 ccacgttcca gggcgactcg gaactgtggc cctggatgcg caccatcctg cgccgcacga
     8581 tgctcgacgc gttccgcagg gccgaaaccc aagtctcgct cgacgatacg gtcgcgagcc
     8641 gcgaaatcga agcgcggatt gccgaattgt cggtcgcacg ggccgatgca cctcccgacg
     8701 cggcgcacgc gcgccagctc gccgagacct tccagcgctg ctggacgagg ttcgagcagg
     8761 actgccctgc gcacgcggcc gtgatcgtat ggatcagcga agacgggctc gacaatgccg
     8821 ccgtcgcgca gctgctcggc cgcacgccag gcgcgacccg tgaatacatc tcgcagtgcc
     8881 gcaagaaact gcgcgtgtac ctggccgact ggttcgagct ggcctttgga gcgccgtcgc
     8941 cgtgactgac gacccgacac tcgacgtctg gttcgatgcg ctcgcgggcc gcggcaccgt
     9001 cggcggcgcc gccgccaacg aggcgcgcgc gctgcgcgag gcgctccggg cgcgacccgc
     9061 catcgatgaa ctgggcctgc agcgcgagct ggctacgagc gatgcagacc gcggcgccca
     9121 actgctcggg aaggcgcgcg gcgacccgat cctcggcccg atacttgcgc gcggccgcgg
     9181 tcgcctgaac attgcgcgcc gcgcttgggg cgggctgctc gctgccggtc ttgccggcat
     9241 ctcgatcgca ctcgtatgga cgctgcggcc ggcaaccgaa gtgcccgtgt atcgcgaggc
     9301 gcccgaccag gttcaccgca tggtcgccgc cgatccgcgt gcgctgcgcg acgaaatcgc
     9361 gcgagcactg cgcgccgccg gcatcgaggt cgtcatctat gagcgcttcg gccgcgaggg
     9421 actcgacgcc gagctgccac gacccgtcac ggggccggtg cgcagcatcc tcgtgctcta
     9481 tcgcatcccg gagcccgccg acggcgtgct gcgcatcgaa atcgaatcgg gatcttcgcc
     9541 atgatggtgc ggcgcggcct tgcggcgttc ctgctggtgg cactagtgat cgccgggcgc
     9601 agcgccgcgg cgctcgagtt gccctcgatc tgcacacagc agaccccacc cgacaacgct
     9661 agcgtaccga tcgcagagtt gcgcgcgcag gtggacgcga tcggcgagag cgagccgatg
     9721 caggcggtag ccatcctctg cgcgacgatt ccgcgcgtgg cgcgcgagta cggcgacgac
     9781 tcgctcgaat tcgcctggtg ggtgcagacg cttgccacgc cgctgatcgc ctaccgggac
     9841 gaattcaccg aagcgattcc gctgctcgaa tacgcgcgcc cgatcatcga gcgcgagctt
     9901 ggcactcaaa gtgcgcagat cgccgagatc catgtcgcgt atgcctggat cgcgtttcgc
     9961 cagggccgga tcgcagaggc cggcacccaa tgggacgcgg cgctgcagat ccgcgagcag
    10021 gtgcccggcg cgaagcagat cgaactgcag aaggcactgg tgggtctcgc gcaagtgcgt
    10081 ctgtcgcagc gccgattcgc ggacgcgcgc gcgctgctcg agcgtgcgca acgcatactc
    10141 actgccaacg gcgagaccac gagcgaagcg gcgggtgcga tcgagaacgt cctgaccaac
    10201 atagcgctgc gcgaggagga ttatccgcgc gcacgccggc acgccgaagc gcaactcgcg
    10261 atcgagttgc agctgcgtgc cgagcgcggc ccagcccagc cggtgaccgc ctggctgctc
    10321 tatggacagg tgctcgagaa gctcgatgaa tatgaacagg ccgagcacgc gatgcgtgaa
    10381 gccgtacggc tcgccgaagc gaccgacggc ccactgcagc ggcattatct gcgggcactg
    10441 accggtctca gtgtgctgct gaacgctcgg ggcaagccgc aggaagcgct gcccttcgcc
    10501 cggcgtgcgg tcgaggtggg cacgcagcag ctcggcgccg atgcgccgaa tcttgtaagc
    10561 gtgctgctgg gcctcgcgga gatccagcgc gtgctcggcg acctgcccgc ttcgcgtgca
    10621 ctttacgatc aggcgggcgg gatcatcgcc acgcataccg ccgacgtcga acgccaggtg
    10681 cgcgtcagtt accaccgggg cctcggcctg ctgctgcagc tgctcggcgc aaccactgag
    10741 gcgcgtgccg ccctcacccg cggcctcgag atggccggcg acggcgccga gatggcaacg
    10801 gaacgagcca atgtgctgct gctgctcgca cgtctcaacg ccgccggcga tccatcgctc
    10861 gctcgcgagc agcttggcga ggcgctcgca ctgtacgctg ctcgcctgcc cgccgggcac
    10921 ccgacgctgc tgcgggtcgt caatgaactg tgcgcgatcg acgtgcgcac gacacccgcc
    10981 gtcgctccga actgcatcga ggcacagcga cggctcgagg ccgcgcgtga agtcgaaccg
    11041 gcccttcgcc attccgtgta cgccaacggg agtgccctcg aggaggcgca cggcaacggt
    11101 ggcggtgcat attcgctcgc ggtacgggcg ctcgccgcgg ccgcgacgct cggcacaccg
    11161 gatcctgaat ggcaggctca gtaccggctg gcgcgcctgc tgcaccagcg cggcgaccac
    11221 gcactcgcga tcttcttcgg caagcagtcg atcggccgcg tcgaggcgct gcgagccggc
    11281 ttcaacgatg ccgatcgctc cctgcacagt ggcttcctgc gcgacaaggt cggtgtctac
    11341 cgcgccgtgg ctgattggct gatggagagc ggacggatgg aggaaggcct ccaggtgctc
    11401 gacctgctga aggccgaaga gctgagcgat ttcgtgccgc gcgcgggccc ggcgacgcgg
    11461 cccggaccag gcattgcgct gaccgctgcc gagcagcaac tctgggcgag ctacctcgcc
    11521 acgttgcgcg ccgattcgcg ggccggcgcc gagatcgata cgctcacccg ccgccgcgaa
    11581 gccgggcgca tcagcccgga cgaacgacgc cggctcgacc agttgctcgc gagccagcct
    11641 ggcgaagaag ccgcgcgagc gaggcgtatc gatgcattca tcggtggcgc ctcgcgcacg
    11701 gctgcgccgc ttcccggccg gatcatttcc accgaccgcc ttgcacggga agtcgagcgc
    11761 ttcggcaatg acagcgccat cggtgtctac ctgctgaccg atcagcacct gcggatactg
    11821 gtggccacgc gcgagggcca ggccgagcac cgcatcgaca tcgaagcgac ggcgctgcgc
    11881 cgcgagatcg gcgacctgct cgacgcgatc ggccggcgcg aagatgtgca cctgcgctca
    11941 cgggcactgt acgacatcct ggtgcgcccg ctcgccgagg ccgccgcccg tgcgggcgcg
    12001 caacggatcg ttttctggcc tgacgacgcg ctgcgctatg tgccgttcgc ggcgctcagc
    12061 gacggcgagc attacctggt cgagcgctac gcgatccagt tgtacgccga cgcggccagt
    12121 cgcgcggccg atgcgccgac cgccgggcgc gagcccgtgc tcgcggtccg cgggctcggc
    12181 gtcacgcagg ctgttgcggg ctaccgtgca ttaccggccg tcgccgacga gctgtgctac
    12241 atcgttcgcg ggccaatcac cgggctcgcc tcgcccggcg aggcctgcga tgcgccgaca
    12301 cccggcgagg gaccctcgac ggggcgcggc gcgttgccgg gtgagggcta cgccgacgcg
    12361 gcgttcaccg aggcgcggct cgactcgctg ctcgcagcgc cgcgcgattt ctcggtgctg
    12421 catctcggca cgcatttcaa cctgcgaccc ggcaatgcga tgcgctccta cctgctgctc
    12481 ggcgatggcg ggcagctcac gctcgacaag atcggcgcgc tcgacttctc ggggatagcg
    12541 ctcactacgc tatcgggctg ccagaccggc atgggcggcg cggtcaccgc cgacggccgc
    12601 gagatcgagg gactcagttc gatcgtccag cggcgcggcg cgcgcagcgt gatcgcaagt
    12661 ctgtggcgag tcgaggacca cagcaccgcg agcctgatgc gcaagatgta cgaggagatg
    12721 tcgacattgc gcacccgcga cggcggcacc gcccgcgcgc tgcagcttgc gcagctgtcc
    12781 ctgctggatc gacaccccta ctactgggcg ggcttcctgc tgacgagcca gtgaggcgcg
    12841 gcacacggcc ctgaccctgc gcgccaggcg ccgagcccta agctttcctg atcttcagcg
    12901 cctggcccat cacgcgcacc ttgcgcagct cgcgcaggat ctccttcggc atgcctgccg
    12961 gcaggtcgac gaagctgtgc tcgccgcgga tgtcgacgcg tccgatgtgc ttgcccgcga
    13021 gccccgcctc gcttgcgatg gcgccgacga tgttggccgg cctcacgccg tgcgcgcggc
    13081 ccacctcgac gcggtaggtt tccatctcga ggtccacggc gcgcccggct tcgcgccgcg
    13141 gacgccgctc cgcctcgccc cgcggacgcc gctccggctc gcgccgggag cgcggttcgc
    13201 gcggcggcgg aggggtacgc ggcttcggct ccgccggcaa cagcagcggc gtctcgccct
    13261 ggacgatcgc ggcgagtgca gcggcgacat cgagatcggt gacgccctgc tctcgcgcca
    13321 cttccgcgac catcgcgtgg aacgcgctgc ccgcgcccga ctcgagcgcc gccttgatgc
    13381 gctgcttgaa gcgcgcgacc cgttgcaggt tcacgtcttc gaccgacggc aactccatcg
    13441 gctcgatcgt ctggcgggtc gcgcgctcga tcgcacgcag caggttgcgc tcgcgcggcg
    13501 cgatgaacag gatcgcctcg ccgctgcgcc ccgcccggcc agtgcggcca atgcgatgca
    13561 cgtaggactc cgggtccgtc ggcacgtcga agttgaccac atggccgatg cgctcgacgt
    13621 cgaggccgcg cgccgccacg tcggtcgcca cgacgatgtc gagttcgccg gatttcagtt
    13681 gcgcgacgat gcgctcgcgc tgcggctgcg gcacatcgcc gttcagggcg gcggcggcga
    13741 agccgcgcgc ctcgagacgc tcggcgagtt ccacagtggc ctgcttggtg cgggcaaaga
    13801 ccagcatgcc gtcgaactcc tcgacctcca gtatgcgggt cagggcgtcg agcttgtgca
    13861 ggccgctcac gatccagtag cgctggcgga tattggccgc ggtctgcgtc ctgccgcgga
    13921 tcgtcacctc gaccggcgag cgcaggtgct tctgcgcaat gcgccggatc ggtgccggca
    13981 tggtcgccga gaacagcgcc acctgccgcg tcgcgggggt ctctccgagg atccattcca
    14041 cgtcgtcgat gaaccccatg cgcagcattt cgtcggcctc gtcgagcacc agcgcgcgca
    14101 ggccatcgag cgcgagcgtg ccgcgcttca tgtgatccat cacgcgcccg ggcgtaccga
    14161 cgatcacgtg cgtgccgcgc ttcagcgcat gcagctgcgg tccgtagccc tggccgccat
    14221 agatcggcat gacgtggaac ccgggcatgt gcgcggcgta gcgctgaaac gcctcggcca
    14281 cctggatcgc gagctcgcgt gtcggcgcca gcaccaggac ctggggcttg cgctgcgcga
    14341 ggtccaggcc cgacagcagc ggcaacgcaa acgccgcggt cttgccggta ccggtctgcg
    14401 cctgtccgac catatcgcta cccgccagca gcaccggaat cgtcgcggcc tggatcggcg
    14461 acggcgattc atagccgaca tccgcaagcg ccgcgagcac cggcccggat agcccgagtt
    14521 cgcgaaaagt cgtcacggtg cgatcggcac gccgatccag cgcgcatgca ggacgaggac
    14581 gaacagcaga tacgcgccca ggcccagcac cacggcgatg aggtcgttcc agcggccggg
    14641 tggcgccgag ggaatcaccc gctgcacgcg gtgcttgaac gagatgcgat cgacgaccgc
    14701 ccaggcgaga aatgcgccga acagcacgac gtcggccagc gtgccgttgg cgagcaggtg
    14761 cgcgagcgcc cacagcttga ccgcggccag catcggatgc ttcaacttcg ccttgattcg
    14821 ccccggcagg taggccgcaa acagcagcgg gaacaccggc agcatcagga gcgcgacgac
    14881 actgcgcagc caagctggcg gctgatacag gacgagcggc tcctggcggg cgaggccgta
    14941 gccccagatg atcagtgcaa gcccgacgag cgagagaatc gagtagaacg cgcgccagcc
    15001 gaggccatac ctcgtggcaa gatattcacg cccggtcggc gagaagatgg cgacggaatg
    15061 agtgccaaag aatacgatta caccgatcac gagccacatc atgtccatac cctcccctgc
    15121 tgacgcgcat cctactccgg aatcggcggc ctgcgagcgc aacaagcagc cgatcctcga
    15181 cctgctgcgc acggccttcg caaggcagcg gcgcgtgctc gagatcggca gcggaaccgg
    15241 ccagcacgcg gtgttcttcg cccgcgaaat gcctcatctc gaatggctgc cgagcgatcg
    15301 cgccgaatgg ctgcccgggc tgcgcgcgcg gatcgcgcag catggcccgc ccaacctggc
    15361 cccgcccgtc gagctcgacg tcacgatgcc ggcttggccc gtcaccggtg tcgacgccgt
    15421 cttctcggcc aacacgctgc acatcatggg cccgcgcgaa gtcgaggcct tcttcgcggg
    15481 agtcagccgc ctgttcgccg ccggggccgc tgcggcacct cccccgcaca ccctcgccgt
    15541 gtacggccct ttcaggtacc ggggcgcctt cacctcggcc agcaacgcgg agttcgacgc
    15601 gttcctgcgc gcccgcgatc cggcgagcgg cattcgcgat ttcgaggtgg tgaacgagct
    15661 tgccgcggcg cagggtctcg cgctgtgcgc cgatcacgcc atgccggcga acaatcagtt
    15721 gctcgtctgg tgcaattcct gacgacggcg cttggcacgt cgcgccgcca tgttgaggcc
    15781 ctcgacgaaa gccgagaacg ccatcgcggc gtacacgtag ccgcgcggca cgtgcacgcc
    15841 gaagccgtcg gcgatcagtg tcgtgccgat catgaggagg aagccgagcg ccagcatcac
    15901 gatggtcggg ttggcgtgga tgaaattcgc gagcggctcg gccgcgagca gcatcgcgat
    15961 gaccgcgacg atcaccgcga tcaccatgat cggaatgtgc tcggtcatgc ccaccgcggt
    16021 gatgatgctg tcgagcgaga agacgaggtc gagcatcagg atctgcgcga tggcggaggc
    16081 gaagccgagg gtcgcctgct tgccgacgag gacgtgcggc gcggcgtccg gatcgacgct
    16141 ctggtggatc tcggtcgtcg ccttccagac gaggaacagg ccgcccgcga tcaggatgat
    16201 gtcgcgccac gagaagccat tgccgaaaag ctcgatcacc ggttccgtga gccgcacgat
    16261 gagcgcgatc gtgccgagca gcaggaggcg cagcaccagc gcgacgccga tgccgatgcg
    16321 ccgcgcggag gcgcgctggt gctccgggag cttgttggtg aggatcgaga tgaagatcag
    16381 gttgtcgatg ccgagcacca cctccatcgc gatgagggtg gcgagcgctg cccaggcagc
    16441 gggttgagtg gcgagatcga gcaactgttc catggcggcc agaatatcag ggtgcaggtt
    16501 cgaggcgcac atcccgcaag tcgaactccg cccccctgta gcccggcgac aggaagatca
    16561 ccacccgcgt gatgtgcgac agcccgagcg ggcggccccg gggcccctgc tcgatctccg
    16621 ccagcggaat gcgccagcgc agcgctgcgc cgggcggcag gacccgctcg cgattgaagc
    16681 gatcctcgta gtcggtgtcc cgcggcaggt catcgatgcg cacgttcagc gacaacgcgc
    16741 gcgagccggg attggcgacg gacacgatca gcgcatcgaa cccgcgccag tccggcggcg
    16801 gctcgcggat gacgatgcct gcataggaat cggtcccggc gacgaaccgc acgccctggc
    16861 catcgggaag cttgctgaag gtaccggtcc gcggcgactc gatgaacacg tcccaggtcg
    16921 cgcggcgggg atcccagatg acatgcggtg cgtcagcacg ctgcgcatac gcccagccga
    16981 tccgtgcgag cggcaggagg ccagccagca gcgccagcgc cgcgaccagg tgcaaggccg
    17041 gcaggcgccg cgtggccgcg aaccacagtg tcacgccgac cgcgccgcca agcaggtccc
    17101 ggccgaagtc gccgaaggtc gcatcgccca cgccaaaacg cccctgggcg agttccgtgc
    17161 cggccgcaag cagcacgatc atcgccgcga ttacggcgag gcgggcgcca cgcggccgca
    17221 ggatcacgca cagcagagcc gtgatcaccg caaacaccag cacgtgcccg gcatcgtgca
    17281 cgccacccat ccaggcggtc gacggcagcg ccggcgggta cacgatcagc gcgagcaaca
    17341 gggcgatggc gcaggcggcg atgacgcgca tggggcaccg agtatgcaat aaactcgcgc
    17401 catgcaacgc gtcaccatat ccctcgacga cgagctcgcc gccagcttcg accggctgat
    17461 cgccgggcgt ggctacgaga accgcagcga agccgtgcgc gacctgatcc gccagcagct
    17521 cgaccgcgag gcgcaggacc acgcgcccca cggccactgc gtcgcgagtc tctcttatgt
    17581 ctacaaccac catgcgcgcg atctcgccga acggatcgcc gagcgccagc acgcgcatca
    17641 cgacctggtc gtcgcgacca tgcacgtgca tctcgaccac gagaactgcc tcgagacggc
    17701 agtgttgcag ggacccgtgg ccgccgtgcg cgagctcgcc ggcagcttga cggccgagcc
    17761 cggcgtgcgt cacggcagcc tgaacctcgt ggccgtggag caggccgagc accatgccca
    17821 cggcgggcgc acccaccggc acatgaagcc taggtactga ccgtaaccac attgtatgac
    17881 gattgttgat tttgtcatac tcggtccacc atggaatctc taccagcctc cttcggcggc
    17941 ctcgccgtcg tcgccttcct gctcggcctg aagcacggct tcgacgccga tcacctcgcc
    18001 accatcgacg gactgacccg cgtgaacacc ggcgccggcc ggccgttcgc gcgctactgc
    18061 ggcgcgctgt tctcgctcgg ccacggcatc gtcgtcatcg cgatcgcgct gctcgtgagc
    18121 acggcagtgg cgcactggca gacgcccgac tggctgcaga cgaccggggt tgcgatctcc
    18181 atcttcttcc tgaccttgct cggcatcctc aatctgcacg ccgtctggcg cgcggcgccc
    18241 gatgaggtgg tcacacccgt gggaatcaag ggccgtttcc tcggcgcgct cgggcgcgcg
    18301 aagaacccgt tcatcgtcgc actcgtcggc gccctcttcg cgctgtcgtt cgacacgatc
    18361 agccaggcgg ccttctttgc actcgccgcc ggacagtacg gtggcgcgat ctacgccgtc
    18421 tcgctcggcg tcctgttcgt gctcggcatg ctggtcaccg acggagtcaa cgggctgtgg
    18481 atctcgcgcc tgatcctgcg cgccgatgaa accgcgcgca tcgcctcgcg ggtgatgagc
    18541 cttgcggtcg cctgcgtcag cctgctggtc gccggcttca gcgcgacaaa gctcctgctg
    18601 ccgcgcatcg acagctgggc ggagggcaag gagctcgtat tcggcgcggt cgtggtcgcc
    18661 gtggtgctgg cgggcttcct gtggggccgc tcggtcgccg gcgcccgcgc cgcgcgcatg
    18721 ccgcgcggcg gcggcgccgt cgcggcggtc gtggccgtgg cgctcacggc gctgatcggt
    18781 gtcgaggccc gcgccggcgg catcaccggc gccggtctcg aggaagtcac cgtgcgcgcc
    18841 aaccgcctgc ccctcaccgg ccggccacgc tcggcgagcg aaggcacggt gctcggcgaa
    18901 cagctcgagt accggccgtt gctgcgggtg ggcgagctgc tcgagacggt gcccggcctg
    18961 atcgtcacgc agcacaccgg cgacggcaag gcgaaccagt acttcctgcg cggcttcaat
    19021 ctcgatcacg gcaccgattt cacgacgcgg gtcgagggca tgccggtgaa catgccgacg
    19081 cacggccacg gccagggcta catggacgcg aacttcgtga tcccggagct cgtcgatcgc
    19141 atcgtctacc gcaagggcac ctactacgcc gatctcggca acttctcggc agtcggcgcc
    19201 gccgaactgc actatgtcga tgccgtgcgg ccgttcgtgg ccgcgagtgc cggcgaggac
    19261 ggctatctgc gcgccctcgc gggcggctcg atgccgcttg ccggcggctc gctgcttgcg
    19321 ggcttcgaat acgatcgtac cgacggcccg tgggtactgc ccgaggacct gaccaaggtg
    19381 aacggcgtgc tgcgctggtc gcgcagcagc gaaacctccg gtctcgcgct cgacctcatg
    19441 gcctccgacg gcgaatggac ttccaccgac cagatcccgc gacgcgcggt cgacagcggc
    19501 gcactcgacc gctacggcta tgtggacccg accgcgggcg gcgagtcgca tcgctactcg
    19561 ctgtcggcga gcggttttcg cagctacggc ccgggccggc tcgatttcag cgcctacgcc
    19621 atggattacc ggctgcagct ctattcgaac ttcacctacg cactcgaccc ggtgaacggc
    19681 gaccagttcg agcagttcga cgaccggcgc gtctacggcg gcgcgctcac ctggtcacag
    19741 cccttcgcgt tcggtgccgg cggcagcgtc tggcgcgccg gcgccgacct gcgcgtggac
    19801 gacatctccc cggtcggtct ctacctgacc acggcccgcg cgcgccacga gacgatccgc
    19861 gaagaccggg tcgagcagac gctcgcgagt gcctggaccg cagttgcgac gcagtggaca
    19921 ccgtggctgc gcagcgaact cggtctgcgc gcggatcgcc tcggctatga cgtcgccagc
    19981 gacctcgcgg cgaactcggg cacgggccac gattcgctcg tgagcccgaa gagctcgctc
    20041 gcgctcggcc cctggcgcga caccgaattc ttcgtcgccg cgggccgcgg cttccacagc
    20101 aacgacgtgc gcggcgccac catcgcggtc gacccgacgg acggagtcac gcctgtcgat
    20161 cgggtcacgc cgctcgcccg cgccgacggc tcggagatcg gcctgcgcac cgcgctcctg
    20221 ccgcgcacgc agctgtcggt ggcgctgtgg cggctggacc tcgactccga gctgctgttc
    20281 atcggcgacg gcggcacgac ggaggccacg cggccgagcc ggcgggaagg cgtggaactg
    20341 agcgtctact cgcgcccccg cgactggctg atcgtggacg cggactatgc ggcctcgaag
    20401 gcgcgcttcc gcgatgccga ccccgccggc gagcggatcc ccggcgccgt cgagcaggtc
    20461 gcctccgtcg gagtcacgct gcagacggcc tcaggctggt tcggcggcgc gcgcctgcgc
    20521 tacctcggcc ccgccgcgct gctcgaagac aacagcgtac gctcatccag ctcgacactc
    20581 gtgaatctcg aagcgggccg gcgcctgggt gagcactggc gcgtctcgct gcagctgctg
    20641 aacgcgttcg atcgccaggt gaacgacatc acctactact acgaatcgcg attgcccggc
    20701 gaggccgccc cggtcgagga cctgcatttc caccccgccg aaccgcgcac gctccgggcc
    20761 acgatgcact acagcttctg aggcgcctgt gagccacttt ccccgccttc tgcgcatgta
    20821 ccaggcagga ggacacaagg atgtttctgt ttcagcgccc cattgccgcc gcgttcgcgg
    20881 gactcgcgac cgcatcgata ctggtcgtcg tcgatctgct gatgcgcctc gcgtgttgtc
    20941 agtgatccgc cgcgaccgcg ccgagcttcg ccatctcgcc gagcacctcg gccgtcttcg
    21001 cctgcatcag cgccacgtcg gcgcggctct cgacattgag gcggatcagc ggctcggtgt
    21061 tcgaggtgcg caggttcatg cgccagcgat cgaactcgag tgacagaccg tcggtgaaat
    21121 ctgccgacag ggcctgcggc tggtacttgg cctggatcgc tgcgatggtc gccggaccgt
    21181 cgggcacgcg ccggttgatc tcgccgctcg ccgggaacag gcgcatgcgc tcaccgacca
    21241 gctgcgacag cgacttgccg gtctcgcaca tcacctgcaa cacgatcagc caggggatca
    21301 tcccgctgtc gcaataggag aagtcgcgga aataatggtg cgcgctcatc tcgccgccgt
    21361 agatgccgtc gacctcgcgc atcacctgct tgatgaacgc atggcccgac ttcgacagca
    21421 cggccttgcc gccgttcctc tcgaccacgt cgatcgtgtt ccaggtgagg cgcggatcgt
    21481 gcacgatgcg cccgcctttc tcgcgcttca ggaacacctc ggcgagcagg cccaccaggt
    21541 aatacccctc gatgaacgtg ccctgctcgt cgaagaagaa gcagcggtcg tagtcgccgt
    21601 cccaggcgat gccgacatcg gcgccactgc gtttgatcgc ctcgatggtc gaagcgcggt
    21661 tcgcctcgat catcgggttc ggcacgccgt tcgggaatga gccgtcggga gcattgttca
    21721 gcttcacgaa atcgtagggc agatgcggct cgagctgatc gacgatcaga cccgcgccgc
    21781 cgttacccgc gttcacgacg accttgagct tgcgcagctt cgcgcgatcg atatagccga
    21841 ggatgtgctc gagatagcgg gcgccggtcg ccagcacgtg ccgcgtgccc cgcttcgccg
    21901 ccgggggcgg caggcgcccc gcctcgatca tcgcgcgcat atccttcagg cccgtgtcgc
    21961 cgctgatcgg ccgcgaaccc tcgcgcacga acttcatgcc gttgtagtcg ggcggattgt
    22021 ggctcgccgt gaccatgatg ccgccgtcca tcgactcggc gaaggtggcg tagtacacgc
    22081 cctccgtgcc gcacaggccg atatcgtgga catcgacgcc gctttcgagc agaccctcgc
    22141 tcagcgcctg cacgagttcg tcgctcgaat ggcggatgtc gcgccccacg atgaccttct
    22201 tcggctgcac gaacgccgca taggcccggc cgatgtcacg cgcgagagtg gtgttgatct
    22261 cgtcggggat gcgcccccgg acgtcgtagg ctttgaatgc gttgaggttc atactaagca
    22321 ttatggcatc gaagccccgg cgcatactga tcctcgacgg ccacccggac ggcggagggc
    22381 ggcgcttcgt ccacgcactg gccgaggcct acgcgcaggg cgcgcgtgag gcggggcacg
    22441 acgtgcgcac gctcgccatc ggccagctcg acattccgct gctgcgcaag ggcgaagact
    22501 tccaggccgg cacgccttcg cgcgaagtgc ggcgctggca ggaagcgatc ggctgggccg
    22561 ggcacctcgt ggtgctctac ccgctgtggc tcggcgacat gccggcggta ctcaaggcct
    22621 tcttcgagca ggtgctgcgg cccggcttcg cgttcgcggc gagcggcgag acgcggctgc
    22681 cgaagaagct gctgacaggc cgcagcgcgc gcgtgatcgt cacgatgggc atgcccgccc
    22741 ttttctatcg ctggtattac cgggcgcaca gcctgaagaa cctcgagcgc aacatccttg
    22801 ccttctcggg cttcgctccg atccgctcga ccgtcgtcgg caccgtcgac ggcaaggccg
    22861 caagccgcac ggcgtggctc gagaaggtgc gccacctggg ccgcagggcc gtctgaccct
    22921 cagccggtcc gctccggacc acacaggggc gcttcacggc cccctatgcg ccgacgccgc
    22981 tgcgacgttt caggctcgcg accgcgcgcg atgccgcctg gccgtcccac agctcgggca
    23041 catgacccag cctgccgacg tgggcgagca ccgagtcgac cccggcgacg agcgcggccg
    23101 gctgcaccag ccggttcgaa ccttcggtca ccgtgaccgg cctctcggtg ttctcgcgca
    23161 gcgtgagaca ggggatgccg aggtaggtcg tctcctcctg cacgccgcct gaatcggtga
    23221 tcaccaagcg cgcgccggtc accaggttca tgaactggat gtacccgagc ggatcgacga
    23281 cacgaaccgc gccctctccc gccaggcgat ccagcaggcc gaactcctgc agcttcttcc
    23341 gcgtccgcgg atgcaccgcg aacacgagcg gcacgcgccg cgagacttcg agcagcgact
    23401 cgacgagcgg cccgagcgcc tcgcgcgtat cgacgttcga agggcgatgc agcgtgacga
    23461 tgccgtagcc ctgcggcgcg agcccgaggt cacggcgggt gccgtcggcc tcgatcgccc
    23521 cgcgcagcat ctcgtagctg tcgatcatga tgttgccgat gcactcgatg cgctcggccg
    23581 gcacaccctc gcgcagcagg ttctcgtcgc cgtcgcgcga gggtgtccac agcacgtcgg
    23641 cgatcgcatc ggtcacgagc cggttgattt cctccggcat gcgccgatcg cggctgcgca
    23701 gcccggcctc gaggtgcacg accggaatcc acagcttcgc gccgaccatc gcgcaggcgg
    23761 cggtcgaatt cacgtccccg accacgacga tccagtcggg cttttcggcc atcgccacct
    23821 tctcgtaggc gatcatcacg ccgccggtct gctcggcgtg cgtgccgctg ccgatctcga
    23881 ggtggaaatg cggcttcggc atcgcgaggt cgcggaagaa cgcatccgac atgttcgggt
    23941 cgtagtgctg gccggtgtgc acgatcgacg gccggcacca ggattccttc gacagggcgt
    24001 ggtagagcgg cgcgatcttc atgaaattgg ggcgcgccgc cgcgatcagg tggatgttct
    24061 tcatcgaccg tagatgtcct cgaagcgcac gatgtcgtcc tcgccgaggt agctgcccga
    24121 ctgcacctcg atcatgtgca gcatgatctt gccggggttc tcgatgcggt gtttcgtgcc
    24181 gatggggata taggtgctct ggttctcctc gagcaggaag gtctcctcgc cgcgggtgat
    24241 gcgtgcggtg ccggacacga cgatccagtg ctcggcgcgg tgatgatgca tctgcagcga
    24301 catctgcgca ccgggattca ccgacaggcg cttgacctgg tagcgctcgc cgttctcgat
    24361 gctgtcgtag ctgccccacg ggcggtacac ctcgcggtgc agggaatgct cgtagcggcc
    24421 gtccttcttc aggcgctcga ccaccttctt cacatcctgc acgcgactct tcggcgcgac
    24481 cagcaccgca tccttggtct cgacgaccac gtgatcgcgc agacccaccg ccgcgaccag
    24541 ccggctctcg gcgtatagat agttgccgct cgagtcctcc acgatcacgt cgccccggca
    24601 gacattgccg gcctcgtcac ggtcgagcgc atcgtagagc gccgaccagg agccgacgtc
    24661 gctccagccc gcatcgagcg acacgaccac cgcgtcgctg gtcttctcca tcacggcgta
    24721 gtcgatcgaa tcgctgcggc aggccgcaaa ggccttggcg tcgatgcgcg tgaaatcgag
    24781 atcgcgcctg gccgacgcca gcgctgcggc gcaggcctcg gcgatatccg gcgcatgctt
    24841 gcgcagctcc tcgagatagc gcgacgcgcg gaacacgaac atgccgctgt tccagcggta
    24901 gttgccggaa ttgacgaact gctccgccac tgcgagcgcc ggcttctcga cgaaggccgc
    24961 gatgcgcgct gcgccggcca ccggcgcacc acgctggata tagccgtagc ccgtctcggg
    25021 cgccgtcggc acgacaccga aagtgaccag gtgcccgagg ccggcggctg ccaccgccgc
    25081 gcgcactgcg gcctggaagg ccggcacgtc gcggatcaca tgatccgccg gcagcacgag
    25141 caggatcgga tcggcaccgg gcgcgttgtc gagcgcgacc tgcgcggcga gtgcaatcgc
    25201 gggcgccgta ttgcgccccg caggctcaag gatgatcgcg cgcggctcgg ccccgatctg
    25261 ccgcagttgc tcggccacca tgaagcggtg cgactcgttg cagacgacga tcggcccgac
    25321 cgcgccggtg cccgtgaggc gcatcgcagt cgcctgaagc atcgtctgct cacccaccag
    25381 gggcagcagc tgcttcgggt acagctcgcg tgagaggggc cagagtcgcg ttccggcgcc
    25441 gccggagaga atcacgggaa tcagcacgga aatccttctt gctacaggcc gttcgggcgc
    25501 gaaatcatag ccactttcag ttcccgattt gctcgattgg ttcatcctgc cgaggcctca
    25561 gcaaccgcca attgaaagca atgctgtcat tgactacaat gcagtcatgg cagtactgac
    25621 gatccgtaat gtcgacgaaa cggtgaagac ccgtcttcgg atgcgcgcgg cccggcatgg
    25681 tcgatcgatg gaggacgagg tgcgccagat cctcaaggcg gcagtcgaag aaaagccgaa
    25741 tagctccccc gggttcgccg ccagcgtgcg tgaggcgttt gccgccttgg gcggtgttga
    25801 tctcgacata ccgccgcgcg aaccgccccg ggagccaccc aggctggacg actgagcgcg
    25861 tgatcgtcgt cgacaccaat gtgctttcgg agttggcccg acatgcgccc gacgcccacg
    25921 tgctgcactg gtttgaggcg caacccgacg gggcccttta cacgacagcg atcacacacg
    25981 cggagatcct cttcgggctt gccctcctcc cccggggcgt gaagcgtgac ctggtggaag
    26041 ccgcgatcac cagggttttc acgggaacat atgccgaccg catactgcct ttcgactcgg
    26101 cggcggcgcc ggagtacgcg cggatttgcg ccgcacggcg ggccgccggt cgaccgatca
    26161 gccagataga cgcccagatt gcagcgatca cgcgcgcgcg acgcgcgcgc cttgcaacgc
    26221 gtgatgttgc cgggtttaaa ggcctcgata tcgaaatcgt gaaccccttc gggaccaccg
    26281 catgacatcg ccggcctgac tttcgccgtt acccctggcc ccgccccacc gcgtaataac
    26341 taaagccgaa gcgctgcagc aactgcggat cgtagagatt gcgcccgtcg aaaatcgccg
    26401 gctgcttcag gcccgccttc aggcgatcga agtcagggct gcgaaactcc tgccactcgg
    26461 tcgcgatcac cagcgcatcg gcgccctcgg cggcctcgta ggctgacgcg cagatcgcga
    26521 ggtcctcgtg cgcaccgaac acgcgccgcg cctcatccgc cgccacggga tcgtaggcgc
    26581 gcacccgcgc gccggcggcc cagagcatct ccatgatggt gcggctcggc gcctcgcgca
    26641 tgtcgtcggt gttgggcttg aacgcgaggc cccacagcgc gaaggtgcga cctttcagat
    26701 cgccgccgaa gtgcttgtcg atcttgccga cgaggaacgc cttctgccgc ccgttcacgc
    26761 tctctactgc ctccagcagc gcgggttgat agcccgccgc gttcgaggag cggatcaacg
    26821 cctgcacgtc cttcggtaag cacgaaccgc cgtagcccgt gccagggtaa atgaagctgt
    26881 agccgatacg cgggtccgaa ccgatgccga cgcgcacctt ctcgatatcc gcgcccacgc
    26941 gctcggcaat gttcgccaac tcgttcatga aactgatctt cgtcgcgagc atcgcgttcg
    27001 cggcgtactt ggtcagctcc gccgagcgca ggtccatcac gatcaggcgg tcgcggctgc
    27061 gcgtgaaagg ctcgtaaaga gcgcgcagca gctcggtggc gcgcggattg tcggtgccga
    27121 cgatcacgcg atcaggcttc aggaaatcgc cgactgcggc gccctctttc aggaattccg
    27181 gattcgaaac gacatcgaac tcgacatcgg cgccgcgctc cttcagtgtc gccgcgatct
    27241 ccgccttgac cttgtccgcg gtgcccacgg gcacggtcga cttgtcgatc acgacccggt
    27301 attcctcgag atgcgcgcca atggtgtgcg cgacagcgag cacatacttc aggtcagccg
    27361 aaccgtcctc gtcgggcggc gtgccgaccg cgatcagctg gaacaggcca tgcttgacgc
    27421 cctccgcggc atcggtcgtg aacctgaggc gcccggcctc ggcgttcttc ttgatcaggg
    27481 tatcgaggcc cggttcgtga atcggcacct cgccgcggct cagccgctca atcttgagcg
    27541 gatcgacgtc gacgcagagg acatcattgc ccgcgtctgc aaggcaggcg ccggttacca
    27601 ggcccacgta gcccgaaccg aaaatggtga cccgcatgga ctgcctttcg tgatcgccgc
    27661 cacttggttg gcgagcggat gaaatccgct actgcaacaa cacctgatca gccggcgtcg
    27721 ctatgcacct gacctgtggc gctccgatgc cacgccgccg gcagatgcgc gatcagcact
    27781 ggcgcccggt tcgtgagcat gacagtaaat acgcccgtca acacgaagag ggcaaattgc
    27841 agcgactcgg gcatgccgac ttcgtgaaac acgagacctg cggccgcaag cgccattgcg
    27901 caagtcacgt acacgatgaa ggcaccgcgc accccgagac ccgagtcgac cagcaggtga
    27961 tgaaagtgtt ccctatcagc cgagaatgga gactgccgcc gcgacacccg ccgaatgatc
    28021 gtccagagaa gttcgaacac gggaagcgcg acaagccaca gcaccgacac gggcgccata
    28081 gctcgtatcg acccttgcgt caagcgcacg cacagcaatg cgaggacgaa gccgatcagc
    28141 gtgcttcccg cgtcacccat gaaacagcgt acgccgcgat tcctgcggat gggcagattg
    28201 aagagcagga atgcgcctac cgcgcccgca agagtaaggc ccacctgcaa cgtaaacggg
    28261 aggcctcctc tccaggctat gagcgaaatg cccaccagcg aaaccaaggc cgtggccccg
    28321 gcaagtccat ccatgccatc gagcatgttg aacgcgttga tggccccgac aacgaaaaac
    28381 agtatggcga tataggtcaa tagcccgtga aattcgatgt caccgtagcc taaagggttt
    28441 ccaaggctga accccgaccc gggaaagagg tgggttgcag ccagcagcgt ggcggcagcc
    28501 tgcaccggaa gtcgcaacca gggtgacagc tggaagcggt cgtccagcag cccggtgact
    28561 accagcagtc cgcaagcagc gatgaaggtg tcggctcgca ggagactggc gggacccagg
    28621 ccgacgccga ccgacaaacc cagaaacatc gcaagcccgc cgacaaccgg gacttcgcca
    28681 tggtgagtct tacggccacc tggcttgtcc acgagatcga tcgctactgc gattggccga
    28741 agggcgagga ccgaaattgt gcagacgagg aaactcgcaa gcgagccaag cgccaaggaa
    28801 accacgcatc accttccgtg catccatgcc aatggcgatc gcgccagcac cgcgcatcac
    28861 gggcgatgcc aacgcaatgg tcctgacacg ataattgaac tcgctatttt acatcacccg
    28921 ccctcgattg gtagggcata gagtgtcaat ttcgggactt gagctgtact cagtccgtgc
    28981 ctctatgtat cttgaaatcg cgcacgagat acgacacgag acacgtgact tccacccaga
    29041 tgccattgcc cgcccgcctt cggcctctca gcccgctctt gcttaccgcg gtgttgctcg
    29101 caggctgcgg cggagactcc tcaaattcgc cgccgaccgc ctatccacaa gcattgacca
    29161 tcgatgagga cgcaacgacc gagatagccc tttccgcaag cgaccgcgaa ggcgacgtgc
    29221 ttacttacgc catcgcgagc ttccccacga atggcaccgt gcagctcgac gcgagttccg
    29281 gacgggtgct ctatgtgccg gatgctgact acgcaggcac cgaccgcttc acgttcacag
    29341 cgcacgacgg ccgtaacatg tcaaccgagg ccaccgtcgc aataacactg cggcagatca
    29401 atgacgcgcc gcggttcccc gacatcccgg agttgaccaa cctcccggac agcccgatca
    29461 cacaatggca gcttgcagtc gtggacccgg agtcaactcc agtccaggtc agcctggccg
    29521 ttgccgaccc ctcgatgctg gaggcaacct acaacaccac gaacgacaca ctggagctca
    29581 ggccactgca gtatggagac acacaggtca cagtgagcgc ctcggatggc gaactgtcga
    29641 cggccaggac gttcgtattt cgcgtcggga aggtgacgcg aaaggtcgag ctcgcgattc
    29701 ctgacccctc acgccaggca atcgtaatca cgaacacact tcagtacgga atcaacgtcg
    29761 gcctgggctg gaacaggaag ataatcttcg aatcgcgtgc agacctggtg aatgccgcgg
    29821 tctccccgct tgaatccgac gaaaatgtca tcgccgacgc gctttggcgc ttcatgatcg
    29881 gacatgtcta ccacaacgag tcgtttgccg cggagcgttg ggagcacgat ccattattgt
    29941 tgatcaactc aatcgggttc ggcctgtgtg atgacgtgtc atccgccttg gcatttcttg
    30001 cgcgagagcg tgggctcgca gcgcgcgtct gggctctcgg agggcatgtg gctcctgaaa
    30061 tcaacgtggc cggacggtgg cagctctatg atccggacat caaggtcgtc taccacgatt
    30121 atgatgcacg catcactaac gttgaaaccc tgagcgcgtt ccctgccttg atcacgtcac
    30181 cggtccagcc gctttacggg gcatcgttct atggctacca gtcgttcata gcggacattt
    30241 acgcatcgca cgacgacaac aatgtggaac cctggtatga cgaggctgcg ccggctgtcc
    30301 cgggcacctt tcagtttccg cctggcgcaa gcgtgcactt tcccggcagc gcagacatta
    30361 cagtcaatga tcacatgggc atgtcggcgc ctgcagtgag cagcctggac ctggtactgc
    30421 ctgcaggcac gcgggcggag ctgaagatgc cgctggtgct tctcgacatc aagggagaag
    30481 gcacaattca gatagggggg cacgactacg tactgggaag ctccgctctc gacgaactgt
    30541 ttgcatcaaa ccgcgcgttc attccgggaa tcacaatcgt cgatgccaaa acgccgctgg
    30601 tgctcacctt tcttctcaac ccgaggaatg cagatctgga gacatccacc tcggtatacc
    30661 tgactggcct cgaggtctgg ggcctcgacg tgactactga gaacttcccc gacggccgcc
    30721 cctcgttcag cgcccctcag attcccactt ttcccttcta gcgcgccacc accctgcaat
    30781 ccgcgcgagg tgcggccaac agcgatgggc cgcgctccga ttgcatcaat aatcaatcac
    30841 ggtcggcagc ggcttgcgca tacgtacgct ccctcgggct tcattcgcgg gcacacctat
    30901 cacggaagtt tccaacgccc agacgtcaag tcccttgacg tcaactacct ctgatgaacg
    30961 catggcaaac tgcattgcat tcacaaaatg cacaagtctc agcccgtcat tggcaagtat
    31021 cagcacggac gtaatgggag catctgtatt ctggattcgg tcgcgcaaac gttccgaacc
    31081 caccgtgaat tcagtaccgt ctattgccac cgtaccgctg ccctgaattt cccacagcat
    31141 caggggcaga gacagcgacc ctgtccaacc tggcggaatt tccagagcca cctggccgta
    31201 ctgcgtaatg agcctcggct ccgaaccatc gtaggccgta ggcggctcag tccaccgccc
    31261 cggaaagacg agacgcgacc ctggtggcag ctcgaacctg ctcccaggca tcgggtcctg
    31321 ggcgagaaaa atgctgtcgc caacgaaatt atcgtcaacc gaggcgtata tctcagccac
    31381 cgtaggtgag taaggaaagt cgtacgacga accctcgaaa atcggggcaa tgggattgac
    31441 tatgaggtcc ggatcgttcg ccagttcttc gacaccggca acccgcccgc ttctgccccg
    31501 gtagtaaaca gcaaggtccg catcgtacat ctgccacatg ccgtcgacgc gcacctctgc
    31561 cacgacgtga ccatccaatc cccaaacccg cgcgtcgtat ccggccgcgc gcgcaatctc
    31621 cacaaacacg gccgatacat gcgcgcagaa tccccagccg agggagttga tcgttaccca
    31681 tgggctgtac aaccagtttc tcgaactgaa cggagcattg tgataggaaa agtcccgcac
    31741 gaaccgccag atctttctcg ggaggctttc tcccggaaac tcgtccggca gtgcgcgtac
    31801 gaaatgcagc atgtcttgca ccgattcaaa taccggcaca ccactgtagg tgaggtgaaa
    31861 agcgaccttg ctcgccgact cattccgtat ctccatcgca gaggttgtcg ggtccggaat
    31921 ggaatactct ctgcgcttca cgacctcccg cgcgtcgtag cgaaactcca ttgaggaagc
    31981 actttgcccg tccgaggcag tcacgattcc cccggtctct ccgacggcca atgcgcgcaa
    32041 tacgagtgcg ccagtgtcct catcaatcca ggcatcggct acgcgtgaat cagcgagttc
    32101 aacagtcaat tcgactgcat ccccatccgg atcagtcaca agtgagtgca gaacgacatc
    32161 ctgcgcctcc ggcgaattca gcacagtgcc gatctcgcgc aggcgcggcg tatcgttgac
    32221 attctgaacg tggatgctga ctgtggcttg ctccgacgag atcccgccac tacttgcaat
    32281 cactccaaag gcatccactc caaagaagtc ctcgagtggg tcgtacacga acgatcccgt
    32341 tgcggcgtca agcgtgagcg agccatgcag aggggcggtt gtcaccgtga atgacaccgc
    32401 gcctgtagcc gcgccagtgg cagacaactg ccctagtaga cgcgtttctt caagtatcga
    32461 aacagcgtgg ctctcgacta cgagagaccc gcgaccctgc gccccgcccc cgcagccgaa
    32521 gaggcttgcc gcggacaatg caagcatccc gcatcggact agtctgctgc tccagccacg
    32581 gccttccggc gtgaataatc gtctcatcgc gcatctccag gcgctgcatg ataattcctt
    32641 atcgagcagg cactccagtt cagtgccttg acccagagtg acccaacagt aaacgactca
    32701 tccacccatg gaaacctccg caggcagggt catcactcac tttctccgat tattcgatac
    32761 gcgtcgcaga tttcacgcag catggagcgg cgggaattgc tgggcagcag ctcatgctct
    32821 tgctgcctcg acagcagcta ccaagctgcc tggaccgttc cggtcttcag agcgcagagt
    32881 ccctgactaa tcacaggccg gcccactcaa cagtcaaaac agcggccgcc gggtcgcaga
    32941 tgaatgcgaa ccggcaattg tcactttgca tctaccgcca caatccggtg tgccgcgcct
    33001 acatcagggc gagttcgccc actctcttcg agactgttac tttctttcag catgtccgat
    33061 cgtgcgaacc attctggcgc gttcgcggct gccatcatat gcaagagcag atctgcgcaa
    33121 tgaagaatct cctgctggcg atcaaacgct cctactcacg gattctcgag tacttcgcac
    33181 tagtatcccg gagaagctgc tcaaaggctg ccgccatcgt gctgatatcg aacatctccg
    33241 ccactctcgc acgggccaat gtggcacgac gccgctgcgt ctccgcatct tcctccaaca
    33301 gttcgtcgat tcccttgact agcgcggtca tgtctccgat cgggacaact atgcccgacg
    33361 tcccgacgat ggcagccgcg tcacctacgt cagtggtaac gcacgggaca ccacaggcca
    33421 ttgactccgc aagtaccgaa ggaaacgcct cgccaaaaga cgggagtacg gagcaatcga
    33481 gcgccgccat cacacgattg atgtcccagc gtgctcccaa caggtgcacg cgatcagcaa
    33541 ttccagcctc agcgatccag gcacacagga ccttgttgtc gctcgtaatt cctgcacctg
    33601 ccatcacgac gtgtagttgt ggccatcgtt cgagcaggtt gcggacggct gcaatgaatc
    33661 ctctgtgatt cttctgtgga tcgaaccggg caatcagacc aatcaagcga ttctcgcctg
    33721 acagattcag ttcaccacga accgccgctc gcgcatgggc atcgggctgg aatcgacgga
    33781 tgtcgatgcc gttgtgaata atggtgaact tatcccgaac atagcctagc tcgatgtgaa
    33841 catcgcgcgc gcgctctgag caagacacga ttacagctgg cagccagcga gacaagatgc
    33901 cattgagtcg tgctatcgcc cgagttgtcc aggcagagtc agtccttgac agcgtgctgt
    33961 tcctgattcc ccagatgact ggcaaacccg cgaggcgggc cgcgatcccg ccgacaagat
    34021 ccgcgtggta catccatgtc gatacgacat cggctccgtg cgccttgagg atccgcgcaa
    34081 ctgaggcaac cgcaaatggg ttcggcagta ccgcattcat tccaagctcg tggaccggca
    34141 cgccgagatc ccggaacttg ggcgcgagta cgccatcgcg cccaagtgag attacgaccg
    34201 agtcgctccg ccaggggctg ctttccagaa gacgcagcaa aaccgactcg gcaccgccca
    34261 tccccaggcc cgttatgacg aacgcaggcc tcaacttgct gcaccgtccc agccgagccg
    34321 ttgtagcaac cggggaatca actcgggacc tcacgtggtg cgagctcctc gaggaggtca
    34381 caccaaaggc cgatgacccg gacctgcgaa taccgttccc ggacagcgcg agagccattc
    34441 tcgcccagct gtttgcgccg aagtggatcg tccatgagcg cacgcagcgc cctctcgagt
    34501 ccaggccaat cgttaagccg ggcgagtgca ccatgtgtcc cgtcacaggt gatttcccgg
    34561 ggcccaaaat cgcagtcaaa tgagaccgcc gggattcccg cagccatggc ctctagcagc
    34621 gcgtttggaa atccctccac tgacgaggtc agcacgaaca ggtctgcgcg ctgcatttcc
    34681 tcccaaagcg agtcggtgtg ccccggcagg aacacacgtc cagcgagccc gcgcgcattc
    34741 acctgatgct ccagttcctg gcgcagcgga ccctctcccc aaatccacag atcccactgg
    34801 ttaaactcct gcttcagatt tgcgaaggta tcaagcagcc caccgaactg cttttccggg
    34861 accaatcgac cgcaggcgat caggcggcga cgcccgccag actccacttc ggccttcgtt
    34921 ccggccagcg acgacggcac gggattggga atgaccacaa ttcggcgaat gcctggcgcg
    34981 cacctcatga cagactctcg cacgccttct gtctgaatca ccacggcatc ggcgaagcga
    35041 tacaaccagc gccgcaggac ccgctgcatg cttgtggcct cccggtcgag tgcggggttc
    35101 gttctctcgg agactacgac aggaaccccg gttccgcggg aagcaatgac gctggcgacg
    35161 ttgacgtgcg ggaggaatga cacaagattg tccggtggtg ctgtgcgcaa tagcctgcga
    35221 agcgcccaca accgggccag gcgagagacg aatctgccgc aacgggctgg caccatatcc
    35281 gccagatata cgaggcgtac cgcgggcgcg aggacatagc tgcactttcc tcgcccggag
    35341 aaactgacga ccagcgtgac attccagcca aggtcggccc agccatttgc gagcgtcgac
    35401 gccacgcgct ctgctcctcc gccctccatg gaactgactg caatgaaaac cttgcgcctg
    35461 gtggtcatgc ttggaggcca gtcagcattt ccccgtagat tcgcagcatg ctggcattgg
    35521 cggattccga cgagaattcc ctttccgccc gccgtcttcc tgcgaggcca aactggtgcc
    35581 ggagcgcgcg gtcggctgca agagcctcaa tagccatcgc cagctgcccg gcgctgcgtg
    35641 gtggtaccag catgccattg tcgccgtcgg ttaccacttc cctgcaacct ggcacgtcag
    35701 tagtgacgat tggacggccg cacgccgcag cttctgccag caccttgggg aatccctccc
    35761 ggtaagaagg gaggcacaca atgctcgtac gcgagaacgt ggccggcatg tcagtgctgt
    35821 gaccccacca ctcgaccacg ccttccttga cccaggccgc cacctgctct gtgccgaccg
    35881 atgttggatt gcctttgtcg ggcattccca ccaatacgca acgaacagcc acaccgcgtg
    35941 acttcaggat cctcgccgcc tcgacaaatt ctgcaacacc tttgtcccaa agcagtcgcg
    36001 caggtaatgc tgccaccggc actccggacg gctccggaga ctgctggaat gcctgcagat
    36061 cgacgcccac gcccggcaag tgcctgatcc tgctcgccgg tacacccgcc ttcgcgagga
    36121 gccgcccgtc atccgggttc tggactatgg tcacgccgcg cgagccgacc atcgcaagca
    36181 gaattcgtac aattgcggag accaaaggcg ccctgctgcg ctcggtgaac aggaagccca
    36241 ttcccgctat ggcattgacg acgttccggc agccgctgag tcgacatgcg agcccgccga
    36301 gcactactgg cttcagcgca acgttatgca caatgtccgg ccgttcgcta cgaatcaatc
    36361 gcgcaaggcg aaggacatcc agtatggcgc gcaacggatt catgccccgc cggtcgaacg
    36421 gatagtcgat tacgcgaatg cccgcggcct ctatggcggg tcgcagttcg ccaatcctgg
    36481 ttgcaacgac cacctcgaat ccgctcgtca ccgcagtacg cgcaagcgca agtcgatgtg
    36541 aatggaaata ccagtcctca gtgaccaata tgagcagctt tggcccggtc actgcgtttc
    36601 cagccaggac tgaaacatca gtacatcc
//
LOCUS       DAOQVY010000005        34970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_1413_length_34970_cov_6.355950, whole genome shotgun sequence.
ACCESSION   DAOQVY010000005 DAOQVY010000000
VERSION     DAOQVY010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 34970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34970
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1413_length_34970_cov_6.355950"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PKN91_01135"
     CDS             complement(<1..691)
                     /locus_tag="PKN91_01135"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNS26702.1"
                     /translation="MLLDVRIFALLLLLPLSGCGSLYLAQAAAGQWGVMAAREPIETV
                     VADPATPQELRARLEQVMAAREFASRELALPDNASYRQYAALDRPYVVWNVVAVPEFA
                     IEPKEWCFPIAGCIAYRGYFSEQRARNYALTLESRGYDVVVGGVAAYSTLGRFADPVL
                     STMMGYGDAQLVGVIFHELAHQVVYVAGDSAFNEAFAVAVEQEGLARWLAWRGELGQL
                     DAWRRSRAAQTQ"
     gene            698..1951
                     /locus_tag="PKN91_01140"
     CDS             698..1951
                     /locus_tag="PKN91_01140"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HNS26703.1"
                     /translation="MRVYLVGGAVRDEALGLPVRERDWVVVGATPEQLTSAGYTPVGR
                     EFPVFLHPRTREEYALARLERKTAPGYRGFVTEFSPEVTLEDDLARRDLTVNAMARSE
                     SGELIDPHGGQRDLEARVLRHVSPAFVEDPVRILRVARFAARFAPLGFSVAAETLALM
                     REMVANGEADALVAERTWQETARALATERPDVYFQVLRDCGALAVVFPEVDALFGVPQ
                     PQKWHPEIDAGVHTLMVLRQAALLSEANTVRFAALTHDLGKGRTARERWPMHHGHEAA
                     GVELVDALCERLRVPNDHRDLARLVARYHCDVHRAFDLRAATILGMLERCDAFRRPER
                     FEELLLACEADARGRQGFEERPYPQATYLRRCQAAARGAVLDEVTRAGLEGPQIGIRL
                     HELRAQAIAAQKARVGVVDPAVQIR"
     gene            complement(1911..3083)
                     /locus_tag="PKN91_01145"
     CDS             complement(1911..3083)
                     /locus_tag="PKN91_01145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNS26704.1"
                     /translation="MQAELTPGPDERAHGQRVLAAVHDAIAAAGGAIPFSQFMALALY
                     APGLGYYSAGARKFGPGGDFTTAPEISPLFGRCIARQCSEVLAATGGAILEFGAGTGA
                     LAETVLEALAAREALPERYLILEVSAELRARQQQRLAALAPALAARVAWLDRLPDAPF
                     TGVMLANEVLDALPCERFVVRAGKALRLGVTRDPHSGALREAVLAEGGAVQAGVPGLP
                     ADLDLAALPEGYRGECCPQLQAWIATVGATLGRGLVLALDYGLPRGALYHPDRRDGTL
                     LCHARHRAHGDALRDVGLQDITAWVDFTAVAEAAADAGLTVAGFSTQAAFLLALGIEA
                     DVARETDTARRAARAGEARRLLMPDEMGEIFKAIALTRDLDAPLSGFALQDLRRQL"
     gene            3082..3825
                     /locus_tag="PKN91_01150"
     CDS             3082..3825
                     /locus_tag="PKN91_01150"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF006598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HNS26705.1"
                     /translation="MGALTGKTVLITGAARRVGAEIARTLHAAGAHVALHYRTSAAEA
                     QALVGELNTQRPHSAVGIEADLLDIANLPRLIDTTLGAFGRLDVLVNNASTFYATPVG
                     EITPAHWDDLLGTNLKVPLFLSQAAAPALRRSSGLILNLVDIHGQRPLRRHTTYSIAK
                     AGLAMLTRSLARELAPEVRVNGIAPGPVMWPAGGVDEKLKAEIVARTQLKRLGSPRDV
                     ALAARYFAADAPFVTGQILAIDGGRSLGD"
     gene            complement(3838..4332)
                     /gene="folK"
                     /locus_tag="PKN91_01155"
     CDS             complement(3838..4332)
                     /gene="folK"
                     /locus_tag="PKN91_01155"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNS26706.1"
                     /translation="MTQVLVAAGSNIDPERHLAVAMRELARAFPGTRFSRAYRNAAVG
                     FKGEDFINLVAAFETDLAPPAILEHLHRIETGCGRERDAPKWAPRSMDLDLLLYGDLE
                     CEAPGYRLPRPDLVKRPYMLGPAAEVAPGMRHPTLGATLGELWERFDRAAHELRPMPL
                     APAE"
     gene            complement(4329..4706)
                     /gene="folB"
                     /locus_tag="PKN91_01160"
     CDS             complement(4329..4706)
                     /gene="folB"
                     /locus_tag="PKN91_01160"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNS26707.1"
                     /translation="MTDDVTDRIFLHGLAVDCVIGFIEWERRIKQTVVIDLEMPVDCA
                     RAAQRDAVEDTLDYKKVAKRVIAFVEASEFQLVETLAERIAQLILADFGLEWVRLSVN
                     KPGAIRGSRDVGVSIERRRAAAQ"
     gene            complement(4706..5737)
                     /gene="tsaD"
                     /locus_tag="PKN91_01165"
     CDS             complement(4706..5737)
                     /gene="tsaD"
                     /locus_tag="PKN91_01165"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNS26708.1"
                     /translation="MRVLAIESSCDESAVAVLDEDRGLLAHVLHSQIDLHRVYGGVVP
                     ELASRDHVRRLVPLVRTALADAASTPADLDGVAYTCGPGLIGALLTGAALARSLAYAW
                     GLPAVNVHHLEGHLLAPLLEPDPPGFPHVALLVSGGHTMLVEVRGIGDYRVLGETRDD
                     AAGEAFDKTAKLLGLPYPGGPELARLALEGRPGAYRFPRPMLDRPGLEFSFSGLKTAV
                     SLVVRAPDFDPARRADVAHGVETAIVATLVAKALRALEATGLDTLVVSGGVGANRRLR
                     AELTAAAARRGARVHYPRIEFCTDNAAMVAVAGLARLRAGERSGLTIDARARWPLDSL
                     APLAQQGTA"
     gene            5831..6037
                     /gene="rpsU"
                     /locus_tag="PKN91_01170"
     CDS             5831..6037
                     /gene="rpsU"
                     /locus_tag="PKN91_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNS26709.1"
                     /translation="MPSVRVRENEFFDAALRRFKRACEKAGVLTELRRREFYEKPTQE
                     RKRKKAAAVKRHLKRNSRDSAPRR"
     gene            6046..6498
                     /locus_tag="PKN91_01175"
     CDS             6046..6498
                     /locus_tag="PKN91_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNS26710.1"
                     /translation="MTLKERITEDMKAAMRAGEKERLGTIRMLQAAIKQREVDERITL
                     DDSQVLAVLEKMVKQRKESITQFTAGGRADLVAKETAELELLQAYLPEQLSDAELDAL
                     VNAAIAQTGASSIKDMGKVMGLVKGQAAGRADMGAVGARIKAKLGAAG"
     gene            6536..8380
                     /gene="dnaG"
                     /locus_tag="PKN91_01180"
     CDS             6536..8380
                     /gene="dnaG"
                     /locus_tag="PKN91_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNS26711.1"
                     /translation="MAGRIPQNFIDDLIGRTDIVELIGARVPLKKAGREFKACCPFHD
                     EKSPSFWVSPDKQFYHCFGCGAHGTALGFLMNYDRLSFPEAVEELASRAGVEVPREAG
                     ASDRDGDSSDALYELNARVAGYFATTLAGDARAREYVAKRGLDAESVARFQLGFATNS
                     WNDALRRFGGDAGKVRQLAELGLVIEREGGQIRDGERHYDRFRDRLMFPIRDARGRVI
                     AFGGRVIGEGEPKYLNSPETALFHKGRELYGMYEVRQSRVKLTRLLVVEGYIDVVRLH
                     QAGVRYAVATLGTATTPEHLKRIFRLVSEVVFAFDGDRAGRAAAWRALLNALPEVREG
                     REIRFLFLPEGHDPDTLVAQEGAEAFERRLEAALPLSEYLVRELATQADLAHADGRAR
                     FAELARPLVARVPAGVYRELLIGRVAEAIKLPATRLAELWKAGGAAPAHSGPDPRAPA
                     ARAAARARARHRGAGRRGLVGQAVQVLLHHPAAAANVDEALIRALEQVEEPGMPFLRE
                     LLGELRARPATNTAQILERWRERPEGPRLAELAAEEVLVAESRAAEREIATALERLVV
                     GARERRIDELLVRSRQGDLTAEEKLELQGLMSAKAPTGAAGGRGLGSH"
     gene            8508..10658
                     /gene="rpoD"
                     /locus_tag="PKN91_01185"
     CDS             8508..10658
                     /gene="rpoD"
                     /locus_tag="PKN91_01185"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNS26712.1"
                     /translation="MTRKQKPNKTAKKPSAPRKAPRTVAKTASRKAVARKAEPKARVQ
                     RAAPARKVEARKANAHKAEPARKVVELPRKAEPKAKSKDEARRTAAVPTSIERRPAAM
                     PEERQSQLKLLIARGKEQGYLTYSQVNDHLPSEIVDPEQIEEIVNTINDMGIPVYEKA
                     PDTEALLASEPSAPTDEEAIEEAAAALAALDAELGRTTDPVRMYMREMGTVELLTREG
                     EIRIAKRIEEGLDAVRLALSMYPPTLDFLGRAYEPVKAGQTRLVDIIVGFIDPNAPDV
                     IAQPQNPTRPDPAAAAKDKDDEEAEDEEAEEEVVDTGPDPEEAARRFASIAKLHAQAL
                     NAIAKHGAKDPKTLKIRKKLSGEFMELKLAPRMFEALIGNLRAQVGEVRQLEKEIMVL
                     AVRDAGMPRKDFITTFPKNETSPRWIAKHIKAGKKYSAGIARLKDDIERRQRKLVTLE
                     EALHLSINDIKEVNREVSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQ
                     EGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLN
                     RISRQMLQEMGREPTPEELAVRMEMPEDKVRKVLKIAKEPISMETPIGDDEDSHLGDF
                     IEDQSVASPIDQATMESLRETTHSVLAQLTPREAKVLRMRFGIDMNTDHTLEEVGKQF
                     DVTRERIRQIEAKALRKLRHPSRSEQLRSFLMED"
     gene            complement(10701..11087)
                     /locus_tag="PKN91_01190"
     CDS             complement(10701..11087)
                     /locus_tag="PKN91_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNS26713.1"
                     /translation="MARQAAGGLAKIGQIAIPVSELDRAIVFYRDTLGMRFMFQAPSG
                     LAFFDCGGVRLMLDVAAKSSPEIRSSLLYFSVDDIDANHELLVSRGVEFEAPPHLVAK
                     LPDHELWMAFFRDPDRNLLALMCEKR"
     gene            complement(11138..11692)
                     /locus_tag="PKN91_01195"
     CDS             complement(11138..11692)
                     /locus_tag="PKN91_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HNS26714.1"
                     /translation="MLRNSNERYGTLSVSLHWLMLLLIAAVYACIELRVNFPRGSDTR
                     EALKNWHFMLGLGVLLLALVRLAVHLWGPKPRIQPDPGRLQDLASRVGHFALYVLMLG
                     MPLAGWLILSAEGDPIPFFGLQLPPLLAPNEALAKDIEEIHATAGTVGYWLIGLHAAA
                     ALFHHYVQRDNTLTRMLPLWRRGA"
     gene            complement(11743..12024)
                     /locus_tag="PKN91_01200"
     CDS             complement(11743..12024)
                     /locus_tag="PKN91_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNS26715.1"
                     /translation="MSRLQVKVVPGASRDSIAGWLGDALKVRVSAPPEKGKANAAVEA
                     LLAEALGVAPPSVRIVAGQSSARKQVEVAGLTAAQIRERLEESSGVMSG"
     gene            complement(12021..12719)
                     /locus_tag="PKN91_01205"
     CDS             complement(12021..12719)
                     /locus_tag="PKN91_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016898901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HNS26716.1"
                     /translation="MRSRHHLEGHSGRRTGLLRAAVLGANDGIVSTASLIVGVAAAST
                     TRDPVVIAAVAGMVSGAISMAAGEYVSVSSQADAEQADLERERLELEKDVESERRELA
                     SIYVRRGLQPELARQVAEQLMEKDALHAHARDELGHSEITAAQPVHAAVISAVSFAIG
                     AALPLAGVLVSTRQTVIPIVAASCLFALACTGALAARAGGASMLKGSARVTFWGIFAM
                     AATAAVGSLFGGVF"
     gene            complement(12963..13580)
                     /locus_tag="PKN91_01210"
     CDS             complement(12963..13580)
                     /locus_tag="PKN91_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004903844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNS26717.1"
                     /translation="MLVARQALQRLKDGNRRFVSETLGSEAVATPSRRREVAAAQEPF
                     AIILGCSDSRVPAEIIFDQGLGDLFVIRVAGNIVAPSQVDSVEFAAGRYDTRLVVVLG
                     HSSCGAILATIEELDRAPDDQSRKLRSIVDRVRPSVEPLLGTPIAADRDALVRRAVRA
                     NIRSSVSQLRHGSQLLEDMIRRKGLLVVGAEYNLDSGVVEFFDED"
     gene            complement(13674..13961)
                     /locus_tag="PKN91_01215"
     CDS             complement(13674..13961)
                     /locus_tag="PKN91_01215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003651201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS26718.1"
                     /translation="MDINVSHEEHGHRGAFFVMQDGKRLAEMTYSRASPTLVIIDHTE
                     VDGSLGGKGVGRRLLDAAVQWARDTGTRVMATCPFAKAQFDRDPSIGDVLA"
     gene            complement(13990..14394)
                     /locus_tag="PKN91_01220"
     CDS             complement(13990..14394)
                     /locus_tag="PKN91_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNS26719.1"
                     /translation="MARITGIGGVFFKSKGDPGALAAWYQKHLGLVLEEFGGAILKWP
                     EDKAEDQGLTVWCLASADSQWFSPSESPFMINYRVDDIGELLTQLRAAGVAILQGPES
                     HENGKFAWIMDPDGNKVELWEPKIWDDRNKAF"
     gene            complement(14422..14817)
                     /locus_tag="PKN91_01225"
     CDS             complement(14422..14817)
                     /locus_tag="PKN91_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26720.1"
                     /translation="MSTHDVSDADRTFRADFDHEAHVRLAYVYLVGGDVDEAVQRIRA
                     ALLAFLQHHGIPATKYHETLTRAWVLAVRHFMDKGPASSAADFIASNPELLDSRIMLT
                     HYSASVLFSPEARAGFVEPDLDPIPRAHR"
     gene            complement(14814..15215)
                     /locus_tag="PKN91_01230"
     CDS             complement(14814..15215)
                     /locus_tag="PKN91_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNS26721.1"
                     /translation="MSDHPVDRWHGILERRDLQGLRELLAADAVFFSPIVHRAQSGRE
                     LTLRYLTAAFGVFFNDSFHYVRQIIGPQDAMLEFEVTIDDTLVNGADIIRWNARGEIA
                     EFKVMVRPLKAIHLIHERMAAALQAHGQEAT"
     gene            complement(15240..15581)
                     /locus_tag="PKN91_01235"
     CDS             complement(15240..15581)
                     /locus_tag="PKN91_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004750994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HNS26722.1"
                     /translation="MTQLPNCPACGSALTYQDGELLVCPECAHEWAPGAAAVPEEGPP
                     VHRDAVGNVLADGDTVTVIKDLKVKGAGAPLKVGTKVRNIRLVDEDHNIDCRIEGFGP
                     MKLKSEFVRKA"
     gene            complement(15603..16265)
                     /locus_tag="PKN91_01240"
     CDS             complement(15603..16265)
                     /locus_tag="PKN91_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNS26723.1"
                     /translation="MRMHGLVAMSITLAAAAAIAGCISEPAPGGDAPPGTAARAVADA
                     SKGARVFAECAGCHGMTGQGTPDGQVPAIAGQHASVIVKQLRDYREGERWDARMESIM
                     QEHRFSDDAQIANVAAYLAGLRMPPAGGGPGGETEIRARGERVYAQSCAACHGPRAEG
                     SDAQLMPRLAGQQYMYLRRQLHDVVDGRRPNIAPDHIKVLAPLDVDDIDGLSEYLSEL
                     AP"
     gene            complement(16299..16371)
                     /locus_tag="PKN91_01245"
     tRNA            complement(16299..16371)
                     /locus_tag="PKN91_01245"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16336..16338),aa:Ile,seq:cat)
     gene            16468..16791
                     /locus_tag="PKN91_01250"
     CDS             16468..16791
                     /locus_tag="PKN91_01250"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HNS26724.1"
                     /translation="MSGEIPPRPAYLLVQGVVTDPEGFKAYNAALPPIYAKFGGAHVS
                     LTPAAKVQVVEGEPESRSIVISRFPSKEAALAFWNSPEYTAAKALRAGKGHFFVTVLE
                     GLPGT"
     gene            complement(16760..17209)
                     /locus_tag="PKN91_01255"
     CDS             complement(16760..17209)
                     /locus_tag="PKN91_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26725.1"
                     /translation="MNGRRVFRWCMVGAALIIGGVAVYFAFAYVATSIAVGNSGLKPF
                     YQQSVRAMWLAFCAQLGLLAIVLLYAAAHPRGISRQMVTVLAFMPMLSTVMLFWSAAS
                     RLGGVLLGAAALLMLVAAFAWPAGPSDAEAAIESQPPVRSPGGPRGP"
     gene            complement(17206..17781)
                     /locus_tag="PKN91_01260"
     CDS             complement(17206..17781)
                     /locus_tag="PKN91_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26726.1"
                     /translation="MVTLSRLVEALSRRWLALGLLALAILFMARRLLEYSARMRLLTG
                     HDVFDLQGTLALADIAAQLTHYTPEAVRLYGRYSGIDFPFPFLTALFWAALAVWGLRR
                     ARPDLLASGAWRRWVPWCFVGCVCDWIENVANAWVIGRYPPLHGGIATIALLAKYGKT
                     VAVGITVVVSMAFALWGAYTSISRFVQRRLT"
     gene            17932..18693
                     /locus_tag="PKN91_01265"
     CDS             17932..18693
                     /locus_tag="PKN91_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26727.1"
                     /translation="MRTTILLLATLPLVVPVALATDAAADSIYSRAVADPARPAKDRE
                     RDARDKPAEVLALAGFGPGMKVADIFAGGGYYSELLGDVVGPGGSVLMLNNTAYQHFA
                     REELKERLKDGRLANVKPMLIESCDLRLGNDDLDGALIVMSYHDLYYADEAGGWAPIN
                     AGRFLDQIHAALKPGGVFLIVDHAAARGTGHSAAQELHRIDEAFAKLDIESHGFRLEK
                     TWDGLRNEADRHDVGVFDPKVRGSTDRFVHVYRRI"
     gene            complement(18690..21236)
                     /gene="hrpB"
                     /locus_tag="PKN91_01270"
     CDS             complement(18690..21236)
                     /gene="hrpB"
                     /locus_tag="PKN91_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HNS26728.1"
                     /translation="MSYAPPAELPVTEVLPALRAALREQRSAILQAPPGAGKSTVVPL
                     ALLAEPWLAGRKLLVLEPRRLAARAVAERMAASLGERVGEQVGYRMRLETRVSRATRI
                     EVITEGVLTRRLQHDPALEDTAAVLFDEFHERSLHADLGLALTLEARAALRADLRIVV
                     MSATLDGEAVAKLIGAAPIVTAQGRQFAVETRYLRSGLPVLPGEADSPERLTSLAVLR
                     ALREETGDILVFLPGAGEIRRVAATLEASTGADVDIRALYGELTGEAQLAALAPAGDG
                     RRRIVLATNLAETSLTIPGVRVVVDSGLVRRAIFDPASGMGRLETRRISRASADQRQG
                     RAGRTAPGVCYRLWGEGAHRSLAAYTPAEILEADLAPLALELAVWGAQDAAQLAWLDP
                     PPAAQLASARDLLRWLGALDREHRVTAHGRAMADLPVHPRLAHMLLRSRGLQATALAA
                     DIAALLGERDLLRGAGRDADLRTRLEALRDRGHGAQDVDRGALERVRRAAQQLRTQIG
                     AGGHAGAAGGAGDSSDVEPGELLALAYPDRIGRRRGEGEGRYALANGRGAQFTRPDTV
                     SGSEFIAIAAIDDAARDARIRLAAPLSRAALEALFTDAIETRDVVEWDARTEAVIARR
                     LRTLGALTLDERALRELPAGAAEAAMLAGVVSLGLDALPWTAAARGLQQRVAFVRAHV
                     PDPAEWPDLGDAALAAHLGEWLAPWLGGITRRAHLARLPMSEILGAQLTHAQRAALSR
                     LAPTHLTMPTGSTLPVDYTDPASPTVAVRLQEVFGLAATPRIADGALPVTFTLLSPAR
                     RPVQVTRDLESFWRSGYAEVRKDLRGRYPRHYWPENPLEAEPTRGVKRRR"
     gene            21296..22141
                     /locus_tag="PKN91_01275"
     CDS             21296..22141
                     /locus_tag="PKN91_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNS26729.1"
                     /translation="MRLGRSRRSTNIEDRRASPGGGTGMKFGIGTLVVLLIGYFMGIN
                     PATLLGLVGGLEGAGPAAPPVVGTPTDAEADLIAAVLAETEDTWGGIFRQGGSQYLPP
                     TLVLFRQSVNSACGFATSAAGPFYCPGDRKVYLDLAFYDELATRFQAPGDFAQAYVLA
                     HEVGHHVQNLVGTLGQVQSAKQRLSEGEQNQLQVRVELQADCYAGIWAHYAERSSGIL
                     EPGDIDEGLRAASAVGDDTIQRRMQGHVVPESFTHGSAEQRMSWFMRGFESGRLESCD
                     TFAAR"
     gene            complement(22150..22530)
                     /locus_tag="PKN91_01280"
     CDS             complement(22150..22530)
                     /locus_tag="PKN91_01280"
                     /inference="COORDINATES: protein motif:HMM:NF016156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNS26730.1"
                     /translation="MNNDARRTLAIAALLVALATALGAFGAHGLRDVLTPERLDVFET
                     GVRYHFYHTLGLLALGVLAQLRPARGLRLATKLIVAGIVVFSGSIYALTFGAPLALGM
                     VTPLGGLLLMLGWILVALTVWSAR"
     gene            complement(22547..23425)
                     /locus_tag="PKN91_01285"
     CDS             complement(22547..23425)
                     /locus_tag="PKN91_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS26731.1"
                     /translation="MSVGAPAITAFHDAATGTCSYIVSDPATRRAAIIDPVLDFDAKS
                     GRTATRSADALLAALRARGDTLEWICETHAHADHLTAAAYLRAQAGGRIAIGAGIRHV
                     LCEFAPLFGFGPECPVDGSQFDHLFADGECFAIGTLEARVIAVPGHTIDSLAYLVGDA
                     AFIGDTLFLPQAGTARTDFPGGDAGRLYDSIGLLYALPEATRLFMCHVYPQPGEAPQF
                     ATTVGEQRSSNIHLRAGTSREEFVALRTARDRELALPALIIPALQVNIRAGALPAPQA
                     NGIAYLRIPLDVFGTP"
     gene            complement(23422..25494)
                     /locus_tag="PKN91_01290"
     CDS             complement(23422..25494)
                     /locus_tag="PKN91_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNS26732.1"
                     /translation="MEPARDITAYRRYWAARFGPAPYLPMSRAEMEALGWDSCDVILV
                     TGDAYVDHPSFGMAIVGRTLEAQGFRVGIIAQPDWHDAEPFKALGRPNLFFGVTAGNM
                     DSMVNRYTSDRRVRSDDAYTPNGAGGRRPDRSVIVYAQRVREGFRDVPIVIGGIEASL
                     RRIAHFDYWSEKVRRSILLDAKADLLVYGNGERQICEIAHRLAAGESIDALTDLRGTA
                     FMRRGMPQGWTEIDATTLELRREGAAASPGVRRADRAHTVIRMPSFEQVAADSDLYAH
                     ASRILHTESNPGNARALVQRHGDVDVWLNPPPIPLTTKEMDWVYERPYQRRPHPTYGD
                     AEIPAYKMIRFSISIQRGCFGGCTFCSITEHEGRIIQNRSEDSVIREIGQVRDTVPGF
                     TGVISDLGGPTANMYRIACRSREIEAACRRPSCVYPGICPNLDTDHSALIGLYRRARE
                     VPGVKKVLIASGVRYDLAIESPEYVKELVQHHVGGYLKIAPEAISEGPLSKMMKPGIA
                     TYDRFKELFERYSKEAGKEQYLIPYFIAAHPGTTDEDMLELALWLKKNGFRADQVQAF
                     LPSPMATATAMYHSGRNPLHRVTRSSEKVVVPKGLKVRRLHKAFLRYHDANNWPVLRE
                     ALRRMGRADLIGNGRHQLVPAWQPAGTGTAGEGRRAAANTRPFRTQHTRPRPARAKPA
                     PRNQPRGR"
     gene            complement(25496..27487)
                     /locus_tag="PKN91_01295"
     CDS             complement(25496..27487)
                     /locus_tag="PKN91_01295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNS26733.1"
                     /translation="MSSSFVSAVAAEPGPLIPRSALFGNPTQTQARLSPDGKHISYIA
                     PRDGVLNVWVAPFGKLAEARPVTRDTKRGIRQHFWSYDNKHVLYLQDQGGDENWRVHS
                     VDIATGNDIDLTPIDGVQAQVIGVSERRPGLVLVGLNDRKREWHDLYEIEIATGERKL
                     VEQNDQEFGGYLEDASLQPRIAVKPGAEYDEMFVRNAKGGWDSLMKYGPEDTATTGPL
                     IVEGEGTTALIRSSVGRDTAALVRVDLGSGAQTVIAASDRADVAGVWIEPLSRKPQAW
                     SANYLVDTLTALTPLVQKDIDLLTKTLGPQFDVVSRTLDDRKWIVVVDDPVSALSSHL
                     YDRDSGSVVKLFDQRPALVGAPLAPMTPLEIRSRDGLTLVSYLTLPKGSDADGNGRPE
                     APLPLVLNVHGGPWARDAYGFDPEHQWLANRGYAVLSVNYRGSVGFGKKFVNAGNLEW
                     AAKMHDDLIDAVDFAVREKITNADSVAIYGGSYGGYATLVGLTFTPDRFACGVDIVGP
                     SNLATLLASIPPYWKAFFEQLAQRVGDPRTPEGRKLLEERSPLTRVSAIAKPLLIMQG
                     ANDPRVKQAESDQIVAAMKAKGLPVTYVLFPDEGHGFARPQNRTSAYAISEGFLAKCL
                     GGRYEAVGDDFVGSSVQVPEGAAFVPGLAGALEAAGGKD"
     gene            complement(27579..28757)
                     /locus_tag="PKN91_01300"
     CDS             complement(27579..28757)
                     /locus_tag="PKN91_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNS26734.1"
                     /translation="MRNLRIALVGGGIAGLSAACALQQRGFRVRVYEQAERLAEVGAG
                     LTVTPNATHVLLALGLGDELARHGMQPARGGARHWRTGRLLAEFERGAGLTERYGAPY
                     LQIHRADLHDALARSVLAHDPQCLLLDHRFESLSQDADGVTIRFANGARAEADVAIGA
                     DGIRSAVRAQLFGAGQPRFTGYIAWRGLVPIDALPPGLVDPASCVSIGPQHTFARYLI
                     RAGRVLNYVALAERSDWQVESWSVRSEVGELLAEFKAWHPEVRTIIAATPPELCFKWA
                     LFDREPLASWTRGRVTLLGDAAHPMLPFLGQGAAMGIEDAMVLARAFELAGSHDEALR
                     LYEATRIGRTTFVMGKSRETGLEYHRADAEDRATHPHTTAESVGLFSYNPVVAPLQGI
                     "
     gene            28891..34473
                     /locus_tag="PKN91_01305"
     CDS             28891..34473
                     /locus_tag="PKN91_01305"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26735.1"
                     /translation="MAKTGPGVIAVHGARQNNLKNLDLEIPLGELVVVTGVSGSGKSS
                     LVFDTLYAEGQRRYVETFSPYARQFLDRMDKPQVERVEGIPPAIAIDQTNPVRSSRST
                     VGTMTELNDHLKLLYARAATLYCERCAEPVRRDTPDTIYADIERRAAAAADPRLVLTF
                     AVPVPKNFAEAEVRALLARQGYTRILDRAGAGAPLEVVQDRFLVSQAERARVIDSLEA
                     ALRHGTGRITVRTPDDTWHYSRELACAHCDIACREPAPSMFSFNSPLGACETCRGFGR
                     VIGVDYGLVIPDETKTLRGGAIKPFQTESNRECQEDLEKYAQLRGIPLDTPWHSLTPA
                     QRSWVIEGEGSWRKKVWYGVRRFFAWLESRAYRMHVRVLLSKYRAYTPCEACGGARLK
                     LPALLARIGSHELADAALGTGGPAARTRFMPHGVAWSDAQLEALPGLSIHDLMLLPLE
                     RVREFIDALVLPGALDVATDQLLGEIRTRMRYLVDVGLGYLTLDRQSRTLSGGEVQRI
                     NLTTALGTSLVNTLFVLDEPSIGLHPRDMGRVIDVMHRLRDAGNSLLVVEHDPQIMLE
                     ADRLLDLGPGAGERGGEIVYYGTPEGIRSAAGSLTGAYLSGERTVSEPQRTAPAAAPV
                     AMPGVAHAVRASGSLVLRGAREHNLKNIDVTIPLRRFVCITGVSGSGKSTLVGNVLHP
                     ALAKAFGKAADAPGTFESLEGAGYLDDVVLVDQSRIGRTTRSTPASYVGALDVIRELF
                     AAAPESLVRRYTPGTFSFNSGTGRCPTCGGNGFEHIEMQFLSDVYLRCASCDGKRFRD
                     EVLEVRLEGAHGRHANIADVLDMTVTEALEFFAQASAVRALVKPLQEVGLEYVKLGQP
                     VPTLSGGEAQRLKLAGHLAELVSPAPKRAAEVRTRGKLFLFDEPTTGLHFDDVAKLLR
                     ALRGLLSAGHSLLVIEHNLDVIRAADWIIDLGPDGGDRGGEVVCSGTPQEVRTHARSH
                     TARALRDYEAALGRPVLAVADTPARAPRPRRDNAIVVHNAREHNLQNVHVRIPRDAFT
                     VVTGVSGSGKSTLAFDILFNEGQRRYLESLNAYARQFVQPAARPDVDAIYGIPPTVAI
                     EQRTSRGGRKSTVATLTEIYHFLRLAWVKLGTQYCPDCDLAIKPQGADALAATIAREY
                     RGQRVRVLAPLVVSRKGYYTDLAKWAAKKGFRELRVDGIDTPTARWPRLSRFKEHTIE
                     LPIGDLRVEPARNAQLRAALDRALEYGKGLVHLLDLDARRAGKVAVFSTRRACPGCGA
                     SFAELDPRLFSFNSPHGWCPHCQGAGTEPGDDGDEFADEDRPVCSECDGERLNRIARH
                     VRLRDRSLPALTRDAVDALAQYFRTLRLAGREAAIARDLLTEIRDRLAFLQRVGLGYL
                     ALDRAAPTLSGGEAQRIRLAAQLGSNLQGVCYVLDEPTIGLHPRDNAVLLAALDELVT
                     HRNTLVVVEHDEETILRADHVIDLGPGAGTRGGRIVAEGRARDLERSPESVTGRYLAN
                     PLHHPARPRRATSTRSPALAIERATLHNLQGVSVRVPLGRLVVVTGVSGSGKSTLARD
                     VLHANLQRIVAGGRTRSRARATLTGCGAIRGSEAIGRVLEVDQTPIGKTPRSCPATYV
                     GFWDDIRRIYAAAPESKLRGYSASRFSFNTAGGRCEACEGQGSQTIEMSFLPDVKVPC
                     DVCRGQRFNPETLSVTWRGRSIGDVLAMSIDAAVEFFSAHAKVHHALSLMQDVGLGYL
                     TLGQQSPTLSGGEAQRIKLVTELAKARTSAGARLRAQPVHTLYVLDEPTVGLHMADVE
                     KLIATLHRLVDAGNTVVLVEHNLDVIAEADWVIDLGPEAGTAGGRIVAQGPPARIARA
                     KTSHTGAALAPFLAERTA"
     gene            complement(34490..>34970)
                     /locus_tag="PKN91_01310"
     CDS             complement(34490..>34970)
                     /locus_tag="PKN91_01310"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNS26736.1"
                     /translation="MFVYHPCDDAMLSVLELEGRGWQPQEKKRRLGADIVTGMDELGV
                     LLAGHSRNAYWYGSQLTIEEARRHVPFANATTLQVAAGALAAIVWAMRNPRRGIVEPD
                     DLDFEDCLDTAIPYLGRMTGEFTNWTPLQGRGELFEERLDIDRPWQLQNVRSRQWRG"
BASE COUNT         5650 a        12251 c        11963 g         5106 t
ORIGIN      
        1 gctgcgtctg cgccgcgcgc gacctgcgcc aggcgtcgag ctggccgagc tcgccgcgcc
       61 acgccagcca gcgtgcgagc ccttcctgtt cgaccgcaac cgcgaaggcc tcgttgaacg
      121 ccgaatcccc ggccacgtag acgacctggt gggcgagctc gtggaagatc acgcccacga
      181 gctgggcatc gccgtagccc atcatcgtcg agagcaccgg gtcggcgaag cgcccgagcg
      241 tggaatacgc cgcgacgccg ccgaccacga cgtcgtagcc gcgcgattcg agcgtgaggg
      301 catagttgcg tgcgcgctgc tcgctgaagt agccgcgata ggcgatgcag ccggcgatcg
      361 gaaagcacca ttccttcggc tcgatcgcga actccggcac cgcgacgacg ttccatacca
      421 cgtagggccg atcgagcgcg gcgtactgcc ggtagctcgc gttgtcgggc agcgcgagtt
      481 cgcgcgaggc gaattcgcgg gccgccatca cctgctcgag tcgtgcacgc agctcctgcg
      541 gcgtggccgg atcggcaacc actgtctcga tcggttctcg tgccgccatc actccccact
      601 ggccggcggc cgcctgcgcg agatacagcg acccgcaccc ggagagcggc agcaagagca
      661 gcaacgcgaa gatgcggacg tcgagtaaca tgcgtgcatg cgagtctact tggtcggtgg
      721 cgccgttcgg gacgaggccc tcggcctgcc tgtccgcgaa cgggactggg tcgtcgtcgg
      781 cgcgaccccg gagcagctca ccagcgcggg ctacaccccg gtggggcggg aattccccgt
      841 tttcctgcat ccgcggacca gggaggagta cgcgctggcg cgcctcgagc gcaagaccgc
      901 gcctggctac cgcggcttcg tcaccgagtt ttcgcccgaa gtgacgctcg aggacgacct
      961 ggcgcgccgc gacctgaccg tgaatgcgat ggcgcgcagc gagagcgggg agctcatcga
     1021 tccccacggc gggcagcgcg acctcgaggc gcgcgtgctg cgccacgtgt cgccggcctt
     1081 cgtcgaggat ccggtgcgca tcctgcgcgt ggcgcgcttc gcggcacgct tcgcgccgct
     1141 cggcttctcg gtggccgctg agacgctcgc gctgatgcgc gagatggtcg cgaatggcga
     1201 agccgatgcg ctggtcgccg agcgcacatg gcaggaaacc gcgcgcgccc tggccaccga
     1261 gcgccccgac gtctatttcc aggtgctgcg cgactgcggc gcgctcgcgg tagtgttccc
     1321 cgaagtcgac gcgctgttcg gcgtgccgca gccgcagaag tggcatcccg agatcgatgc
     1381 cggcgtgcac acactgatgg tgctgcgcca ggctgcgctg ctgagcgagg ccaacacggt
     1441 gcgcttcgcc gcactgaccc acgacctcgg caagggccgc acggcgcgcg agcgctggcc
     1501 gatgcaccac ggtcacgagg ccgcgggcgt cgagctcgtc gatgccctgt gcgagcggct
     1561 gcgcgtgccg aacgatcacc gcgacctggc gcgcctcgtg gcgcgctatc actgcgacgt
     1621 gcaccgcgcc ttcgacctgc gcgccgcgac gatcctcggc atgctcgagc gctgcgacgc
     1681 attccgccgc cccgaacgct tcgaggaact gctgctcgcc tgcgaggccg atgcccgcgg
     1741 tcgccagggc ttcgaggagc gtccctatcc gcaggcgacc tacctgcgcc gctgccaggc
     1801 cgcggcgcgc ggcgcagtgc tggatgaagt cacgcgcgcg ggcctcgagg gtccgcagat
     1861 cgggatccgc ctgcacgagc tgcgcgcaca ggcgatcgcc gcacagaaag ctagagttgg
     1921 cgtcgtagat cctgcagtgc aaatccgctg agcggtgcat cgagatcgcg cgtgagcgcg
     1981 atcgccttga agatctcgcc catttcgtcc ggcatcagca atcgccgcgc ctcgccggcg
     2041 cgtgccgcgc gccgggcggt gtcagtctcg cgcgcgacgt cggcctcgat gccgagcgcg
     2101 agcaggaatg ccgcctgcgt cgagaacccc gcgacggtga gccccgcatc ggccgccgcc
     2161 tctgcgaccg ccgtgaaatc gacccaggcg gtgatgtcct gcaggccgac atcgcgcagc
     2221 gcatcgccat gcgcgcgatg cctcgcgtgg cacagcagcg tgccgtcgcg ccggtcgggg
     2281 tggtacaggg cgccgcgcgg cagaccgtag tcgagcgcga gcacgagtcc gcgcccgagg
     2341 gtcgcgccga ccgtcgcgat ccacgcctgc aactgcgggc agcactcgcc gcggtagcct
     2401 tcgggcaggg ccgcgaggtc gaggtccgcc ggcagacccg gcacgcccgc ctgcacggcg
     2461 cctccctcgg cgagcaccgc ctcgcgcagc gcgcccgagt gcggatcgcg tgtgacaccg
     2521 agccgcagcg ccttgcctgc gcgcacgacg aagcgctcac agggcagcgc atcgagcact
     2581 tcgttggcca gcatcacgcc ggtgaacggt gcatcgggca gccggtcgag ccacgcgacg
     2641 cgcgcggcga gcgccggcgc gagcgcggca aggcgctgct gctggcgcgc gcgcagctcg
     2701 gcgctcacct cgaggatcag gtagcgctcc ggcagcgcct cgcgcgcggc gagcgcctcg
     2761 agcaccgtct cggcgagcgc gcccgtgccg gcgccgaact cgaggatagc cccgccggtc
     2821 gcggcgagta cctcgctgca ctggcgcgcg atgcagcggc cgaacagcgg cgagatctcg
     2881 ggcgccgtcg tgaaatcgcc gcccggaccg aacttgcgcg cgccggcgct gtaatagccg
     2941 agccccggcg catagagcgc gagcgccatg aactgcgaga acgggatcgc cccgccggcc
     3001 gccgcaatgg cgtcgtgtac cgcggcgagc acgcgctgcc cgtgtgcccg ctcgtccgga
     3061 cccggtgtaa gctcagcctg catgggcgca ctcacgggca agacagtatt gatcaccggc
     3121 gcggctcggc gcgtgggggc cgagatcgcc cgcacgctgc acgccgccgg cgcccacgtc
     3181 gccctgcact accgcacttc ggcagccgag gcgcaggcgc tcgtcggcga gctgaatacg
     3241 cagcgcccgc actcggcggt cggcatcgag gccgacctgc tcgacatcgc caacctgccg
     3301 cggctcatcg acaccacgct cggcgccttc ggccggctcg acgtgctcgt caacaacgcc
     3361 tcgaccttct atgcgacgcc ggtcggcgag attacgccgg cgcactggga cgacctgctc
     3421 ggcaccaatc tcaaggtgcc gctgttcctc tcgcaggcgg ctgcgcccgc actgcgccgg
     3481 tcgagcggac tcatcctgaa cctcgtcgac atccacggcc agcggccgct gcggcggcac
     3541 acgacctact cgatcgccaa ggcgggactt gcgatgctca cccgctcgct ggcgcgtgag
     3601 ctggcccccg aggtgcgggt gaacggcatc gcaccgggcc cggtgatgtg gcccgcgggc
     3661 ggcgtcgacg agaagctcaa ggccgagatc gtcgcccgca cccagttgaa gcgcctcggc
     3721 tcgccgcgcg acgtggcgct cgcggcgcgc tatttcgcgg ccgatgcgcc gttcgtcacc
     3781 gggcagatcc tggcgatcga cggcggccgc agcctcggcg actaggcgca cgcccgctca
     3841 ctccgccggc gcgagcggca tgggccgaag ctcgtgcgcc gcgcgatcga agcgctccca
     3901 cagttcgccg agcgtcgcac ccagcgtggg atggcgcatc ccgggcgcga cctcggccgc
     3961 gggcccgagc atgtaggggc gcttcacgag atccgggcgc ggcagacggt agccgggagc
     4021 ctcgcactcg aggtcgccat acagcagcag gtcgaggtcc atcgaacgcg gcgcccactt
     4081 cggcgcatcg cgctcgcgcc cgcagcccgt ctcgatccgg tgcaggtgct cgaggatggc
     4141 gggcggggcg agatcggtct cgaacgcggc gaccaggttg atgaagtcct cgcccttgaa
     4201 gcccacggcg gcattgcggt acgcgcgcga gaagcgcgtg ccgggaaagg cgcgcgccag
     4261 ctcgcgcatc gcgacggcga gatggcgttc ggggtcaatg ttgctgccgg ctgcgacgag
     4321 gacctgcgtc attgggcggc ggcccgccgg cgctcgatgc tgacgccgac gtcacgcgag
     4381 ccgcggatag cgccgggctt gttgacggac aggcgcaccc actcgagccc gaagtcggcg
     4441 aggatcagct gcgcgatgcg ctccgcgagc gtctcgacca gctggaactc gctcgcctcg
     4501 acgaaggcga tcacgcgctt ggcgaccttc ttgtagtcga gcgtgtcctc gacggcgtcg
     4561 cgctgcgcgg cgcgggcaca atccaccggc atctcgaggt cgatgaccac cgtctgcttg
     4621 attcgccgct cccactcgat gaagccgatc acgcagtcga cggcgaggcc gtgcaggaag
     4681 atccggtcgg tcacgtcgtc ggtcatcagg ctgttccttg ttgcgcgagc ggcgcgaggc
     4741 tgtcgagcgg ccaccgtgcg cgtgcatcga tggtgagtcc cgagcgctcg cccgcgcgca
     4801 gccgcgcgag gcccgcgacc gcgaccatgg ccgcattgtc ggtgcagaac tcgatgcgtg
     4861 gatagtgcac acgcgcgccg cgccgcgcag ccgcggcggt gagctcggcg cgcaaccggc
     4921 gattggcgcc gacgccgccg gagacgacca gcgtgtcgag gccggtggcc tcgagcgcgc
     4981 gcagcgcctt cgcgaccagc gtggcgacga tcgccgtctc gacgccgtgg gccacgtcgg
     5041 cgcgccgcgc gggatcgaag tccggcgccc tcacgacgag gctcacggcg gtcttgaggc
     5101 ccgagaagct gaattcgaga ccgggccggt cgagcatcgg acgcgggaac cggtacgcgc
     5161 caggccggcc ctcgagcgcg agccgtgcga gttcggggcc cccgggatac ggcagaccga
     5221 gcaacttcgc ggtcttgtcg aaggcttcgc cggccgcgtc gtcgcgcgtc tcgccgagca
     5281 cgcggtagtc gccgatgccg cgcacttcga ccagcatcgt gtgcccgccc gaaacgagca
     5341 gcgccacgtg cgggaagccc ggtggatccg gctcgaggag cggggccagc aggtggcctt
     5401 cgaggtgatg cacattgacc gccggcagcc cccaggcgta ggcgaggctg cgtgcgagtg
     5461 ccgcgccggt cagcagggcg ccgatcagcc ccggtccgca ggtataggcc acgccgtcga
     5521 ggtcggccgg ggtggatgcg gcatccgcga gcgcagtgcg cacgagtggc acgagccggc
     5581 gcacatggtc gcgcgaggcg agttccggca cgacgccgcc atagacgcgg tgcaggtcga
     5641 tctgcgagtg caggacgtgc gcgagcaggc cgcggtcctc gtcgaggacc gccaccgcac
     5701 tctcgtcgca ggacgattcg atggcgagca cacgcatttt gcgtttttac cgggagatca
     5761 ttagaattgc cgcccttgcg cccatgggcc gcgagcgaaa tttctgtaaa ttcaaagagt
     5821 tgaggttccg atgccgagcg ttcgtgtccg cgaaaacgag ttcttcgacg ccgcgttgcg
     5881 ccgtttcaag cgggcgtgcg agaaagcggg cgtcctcacg gagctgcgcc gtcgggaatt
     5941 ctacgaaaag ccgacccagg aacggaaacg caagaaggcg gcggccgtga agcgccacct
     6001 gaagcgcaac tcccgcgaca gcgcgccacg gcgctgacac ccgcgatgac gctcaaggag
     6061 cgcatcaccg aagacatgaa ggccgcgatg cgtgccggcg agaaagagcg cctgggcacg
     6121 atccgcatgc tgcaggctgc catcaagcag cgcgaggtcg acgagcgcat cacgctggat
     6181 gacagccagg tgctcgcggt cctcgagaag atggtcaagc agcgcaagga atcgatcacg
     6241 cagttcacgg cgggcggccg cgccgacctc gtcgcaaagg agaccgcgga gctcgaactg
     6301 ctgcaggctt acctgcccga gcaactgtcc gacgccgagc tcgacgcgct ggtgaatgcc
     6361 gcgatcgcgc agaccggcgc aagttcgatc aaggacatgg gcaaggtcat gggcctcgtg
     6421 aaaggccagg ccgcgggccg cgccgacatg ggcgccgtcg gtgcccgcat caaggcgaag
     6481 ctcggcgccg ccggctgatc ccggctgccg cgcgccgcgt ggcttactat tcggcatggc
     6541 cggccggatc ccccagaact tcatcgacga cctgatcggg cggaccgaca tcgtcgaact
     6601 gatcggcgcg cgcgtgccgc tgaagaaggc gggccgcgag ttcaaggcct gctgcccatt
     6661 ccacgacgag aagtcaccct cgttctgggt gagccccgac aagcagttct accactgctt
     6721 cggctgcggc gcgcacggca cggcgctcgg cttcctgatg aactacgacc ggctcagctt
     6781 tcccgaggcg gtcgaggagc tcgcctcgcg cgccggggtc gaagtgccgc gcgaagccgg
     6841 cgcgtccgac cgcgacggcg actcgagcga cgcgctgtac gaactcaatg cgcgcgttgc
     6901 cggctacttc gccacgacac tcgccgggga tgcgcgcgcg cgcgaatacg tcgcgaagcg
     6961 cggcctcgac gccgaatcgg tggctcgctt ccagctcggt ttcgcgacca attcctggaa
     7021 cgatgcgctg cgccgcttcg ggggcgatgc cgggaaggtc cgccagctcg ccgaactcgg
     7081 gctcgtcatc gaacgcgagg gcgggcagat ccgcgacggc gaacgccatt acgaccgctt
     7141 ccgcgaccgg ctgatgttcc cgatccgcga tgcgcgcggg cgcgtgatcg ccttcggcgg
     7201 gcgcgtgatc ggcgagggcg agccgaagta cctcaattca cccgagacgg cgctgttcca
     7261 caagggccgc gaactctacg ggatgtacga agtgcgccag agccgcgtca agctcacgcg
     7321 gctgctcgtc gtcgagggtt atatcgacgt ggtgcgcctg caccaggcgg gcgtgcgcta
     7381 tgcggtcgcg acgctcggca cggcgacgac gcccgagcac ctgaagcgca tcttccgcct
     7441 ggtgagcgaa gtggtattcg cgttcgacgg cgatcgcgcc ggccgcgcgg cggcctggcg
     7501 cgccttgctg aatgccctgc ccgaagtgcg tgagggccgc gagatccgct tcctgttcct
     7561 gccggagggc cacgaccccg acacgctcgt cgcgcaggag ggcgccgaag ccttcgagcg
     7621 tcgcctcgag gcggcgctgc cgctgtccga gtatctggtg cgcgagctgg ccactcaggc
     7681 cgatctcgcg cacgccgacg ggcgggcgcg cttcgccgaa ctcgcgcgac cgctggtcgc
     7741 gcgggttccc gccggcgtgt accgcgagct gctgatcggg cgtgtcgcgg aggcgatcaa
     7801 gctgccggcg acacggctcg cggagctgtg gaaagcaggc ggcgcggcac cggcgcacag
     7861 cggccccgac ccgcgcgcgc cggcggcgcg cgctgccgcc agagcgcgcg cgcgacatcg
     7921 cggtgcggga cggcgcggac tggtcggcca ggcggtgcag gtcctgctgc atcatccggc
     7981 cgcggccgcg aatgtagacg aggcactgat ccgtgccctc gaacaggtcg aggagcccgg
     8041 catgcccttc ctgcgcgaac tgctcgggga gttgcgcgcg cggccggcca cgaacacggc
     8101 gcagatcctc gagcgctggc gggagcggcc cgagggcccg cgcctcgccg agctcgccgc
     8161 cgaggaggtg ctggtcgccg agtcccgcgc cgcggagcgc gaaatcgcga cagcgctcga
     8221 gcgcctggtg gtcggggccc gcgagcgccg tatcgacgag ctgctcgtcc gcagccgcca
     8281 gggagacctg acggcagaag aaaagcttga acttcaaggg cttatgtcag ccaaggcgcc
     8341 gactggagcg gccggtggcc ggggcctcgg cagccattga cgacgaaatc agtggaattc
     8401 gggaagtgcc ggcgggtctt tagtataatg atgagctttt cctcgccacc ccttgattac
     8461 cgtccccgac cgcgcgggag ggtgcgagca tatatttggc acggaacatg acacgcaagc
     8521 aaaagccaaa caaaaccgcc aagaagccga gcgcccccag gaaagctccc cggaccgtcg
     8581 ccaagaccgc gtcccgcaag gccgtggcgc gcaaggcgga gccgaaggcc cgggtacagc
     8641 gcgccgcacc ggcacgcaag gttgaggccc gcaaggcgaa cgcacacaag gccgagcccg
     8701 cgcgcaaggt cgtcgaactg ccgcgcaagg ccgaaccgaa ggcgaagagc aaggacgagg
     8761 cgcgcaggac cgcggccgtg ccgaccagca tcgagcgtcg tcccgcggcg atgcccgagg
     8821 agcgccagtc gcagctcaag ctgctgatcg cgcgcggcaa ggaacagggt tacctcacct
     8881 actcgcaggt gaacgaccac ctgccgtcgg agatcgtcga ccccgagcag atcgaagaga
     8941 tcgtcaacac gatcaacgac atgggcatcc cggtgtacga gaaggcaccg gataccgaag
     9001 ccctgctcgc ctccgagccg tcggcgccga ccgatgagga agcgatcgag gaagcggccg
     9061 ccgcgctggc cgcgctcgac gccgaactcg gccgcaccac cgatcccgtg cgcatgtaca
     9121 tgcgcgagat gggcaccgtc gaactgctga cccgcgaggg cgagatccgc atcgccaagc
     9181 gcatcgagga aggcctcgat gccgtgcgcc tcgcgctgtc gatgtatccg cccaccctgg
     9241 atttcctcgg ccgtgcctac gagccggtga aggcgggcca gactcgcctc gtcgacatca
     9301 tcgtcggctt catcgacccg aacgcgcccg acgtgatcgc gcagccgcag aacccgacgc
     9361 gaccggaccc ggccgccgcc gcgaaggaca aggacgacga agaggcggaa gacgaggaag
     9421 ccgaagagga agtcgtggac accggcccgg atccggagga agcggcacgc cgcttcgcct
     9481 cgatcgccaa actgcacgca caggcgctga atgcgatcgc caagcacggc gccaaggacc
     9541 cgaagaccct caagatcagg aagaagctct cgggcgagtt catggagctc aagctcgcgc
     9601 cgcgcatgtt cgaggcgctg atcggcaacc tgcgcgcgca ggtcggcgaa gtgcgccagc
     9661 tcgagaagga aatcatggtg ctcgcggtgc gcgatgccgg catgccgcgc aaggacttca
     9721 tcaccacctt cccgaagaac gagaccagcc cgcgctggat cgcgaagcac atcaaggccg
     9781 gcaagaagta ttcggccggc atcgccaggt tgaaggacga catcgagcgc cgccagcgca
     9841 agctggtcac gctcgaggaa gcgctgcacc tgtcgatcaa cgacatcaag gaagtgaacc
     9901 gagaggtctc gatcggcgag gccaaggcgc gccgcgcgaa gaaggaaatg gtcgaggcca
     9961 acctgcgcct cgtgatctcg attgcgaaga agtacaccaa ccgcggcctg cagttcctgg
    10021 acctgatcca ggaaggcaac atcggcctga tgaaggccgt cgacaagttc gagtaccggc
    10081 gcggctacaa gttctccacc tatgcgacgt ggtggatccg ccaggccatc acgcgctcga
    10141 tcgccgacca ggcgcgcacc atccgcatcc cggtgcacat gatcgagacc atcaacaagc
    10201 tgaaccgcat ttcgcggcag atgctgcagg agatgggccg cgagccgacg ccggaagagc
    10261 tcgccgtgcg catggagatg cccgaggaca aggtcaggaa ggtgctcaag atcgccaagg
    10321 agccgatctc gatggaaacg ccgatcggcg acgacgagga ttcgcacctc ggcgacttca
    10381 tcgaggacca gtcggtcgcc tcgccgatcg accaggcgac gatggaatcg ctgcgcgaga
    10441 ccacgcactc ggtgcttgcg cagctcacgc cccgggaggc gaaggtgctg cgcatgcgct
    10501 tcggcatcga catgaacacc gaccacaccc tcgaggaagt gggcaagcag ttcgatgtca
    10561 cccgcgagcg catccgccag atcgaggcga aggcgctgcg caagctgcgc catccgtccc
    10621 gctcggagca gctgcgcagc ttcctgatgg aagactgacg cgactggcgg gcacgggtcg
    10681 gtaccccgtg cccgttcgcg tcagcgcttc tcgcacatca gcgcgaggag attccggtcg
    10741 ggatccctga agaacgccat ccagagctcg tggtcgggca gcttcgccac gagatggggc
    10801 ggcgcctcga actcgacgcc gcgtgacacc aacagctcgt ggttcgcgtc gatatcatcg
    10861 acactgaagt agagcagcga agagcggatc tcgggcgagg acttcgccgc cacgtccagc
    10921 atcaggcgca cgccaccaca gtcgaagaaa gcgagccccg agggcgcctg gaacatgaag
    10981 cgcatgccga gcgtgtcgcg atagaagacg atcgcgcggt cgagttccga taccgggatc
    11041 gcgatctggc cgattttcgc caggccgcca gctgcttgcc gagccatcgc acacctcctg
    11101 tgtcgcgctg ccgacgaaag tggcgccggc gcggtggcta tgcgccccgg cgccagagcg
    11161 gcaacatgcg cgtcaacgtg ttgtcgcgct gcacgtaatg gtggaacagc gccgcggcgg
    11221 catgcaggcc gatgagccag tagcccacgg tccccgccgt cgcgtgaatc tcctcgatgt
    11281 ctttcgcgag tgcctcgttc ggcgccagga gcggcggcag ctgcaggccg aagaacggaa
    11341 tcggatcgcc ctccgcgctc agaatcagcc agcccgccag cggcatgccg agcatcagga
    11401 cgtagagcgc gaagtgcccg acccgtgacg cgagatcctg caggcgtccg ggatcgggct
    11461 ggatgcgggg cttggggccc cacaggtgga ccgcgaggcg cacgagcgca agcagcagca
    11521 ccccgagccc cagcatgaag tgccagttct tgagtgcctc gcgcgtatcg ctgccgcgcg
    11581 ggaaattcac ccgcagctcg atgcacgcat agaccgcggc gatcagcaac agcatcagcc
    11641 agtgcagcga caccgagagc gtgccgtagc gttcgtttga attgcgcaac atgtcagcca
    11701 ccccgggtgg tgaggatcgt agaggggata ccgatgcgga tcttacccgg acatcacgcc
    11761 ggacgactct tcgagtcgct cgcgaatctg cgcggcagtg agccccgcga cctcgacctg
    11821 cttgcgtgcc gaggactgtc ccgcgacgat ccgcacgctt ggcggcgcca cgccgagcgc
    11881 ctcggcaagc agcgcttcga cggccgcatt cgccttgccc ttctccgggg gcgcgctgac
    11941 ccgcactttc agcgcatcgc ccagccagcc ggcgatactg tcgcgcgacg caccgggcac
    12001 caccttcacc tgcaggcgac tcaaaacacg ccgccgaaca gggagcccac ggccgccgtg
    12061 gcggccatgg cgaagatgcc ccagaaggtg acgcgcgccg agcccttgag catgctcgca
    12121 ccgcccgcgc gcgccgcgag cgcaccggtg caggccagcg cgaagaggca gcttgcggcg
    12181 acgatcggga tgacggtctg ccgggtactg accagcacgc cggcgagcgg cagcgccgcg
    12241 ccgatcgcga aactcacggc cgagatgacc gccgcgtgca cgggctgggc agcagtgatc
    12301 tcgctgtgcc caagttcgtc gcgcgcgtgg gcgtgcaggg catccttttc catcagctgc
    12361 tccgcgacct gcctcgcgag ctccggctgc agtccgcgcc gcacgtagat cgaggcgagt
    12421 tccctgcgct cggactccac gtccttctcg agctcgaggc gttcgcgctc gagatccgct
    12481 tgctccgcat cggcctgcga gctgaccgaa acgtactcgc ccgcggccat cgagatcgca
    12541 cctgagacca tgccggcaac cgccgcgatc actaccgggt cgcgcgtggt actggcggcg
    12601 gctacgccga cgatcaggct ggcggtggac acgatgccgt cattggcacc gagcacggcc
    12661 gctcgcagca agccggtgcg gcgccccgag tgcccctcca ggtgatgtct gctgcgcata
    12721 aagtatctta gcatcgtgcg caccgccgac tacgccgcca gacgcctgct ccaccgggtc
    12781 gcgacccatg ccatcggcac accaatgcaa atgatatgca ctgcgatgcc gatggcgagt
    12841 gtcgctggcg cgtccatgcc aatcgccgtg cgaaggcgca gtaaatccag gcgatcagca
    12901 gcatcatcgc gaaatgcagc acgaggccga ggaacgcgat cggccagccg cccgctgcca
    12961 tatcagtcct cgtcgaagaa ctcgacgacg ccgctgtcga gattgtattc cgcgccaacg
    13021 accaacaggc ccttgcgccg gatcatgtcc tcgagcagct gcgatccgtg ccgcagctgg
    13081 ctcaccgacg agcggatgtt ggcccgcacg gccctgcgca ccagtgcatc gcgatcggcg
    13141 gcgatcggcg tgccgagcaa tggctcgacg gagggccgga cccgatcgac gatcgagcgc
    13201 agcttgcgcg actggtcatc gggcgcgcga tcgagttctt cgatggtcgc gaggatcgcc
    13261 ccgcagctgg aatgcccgag caccacgacc agccgcgtgt cgtagcgtcc ggcggcgaac
    13321 tcgacgctgt cgacctgcga gggcgcgacg atgttgccgg cgacgcggat cacgaacagg
    13381 tcgccgagac cctggtcgaa gatgatctcg gccggcacgc gcgaatcgga acagccgagg
    13441 atgatggcga agggctcctg cgccgcggcc acctcccggc ggcgcgatgg cgtcgccacg
    13501 gcttcactgc ccagtgtttc ggaaacgaag cgcctgttgc cgtccttcag ccgctgcaac
    13561 gcctgtcttg ccaccagcat cgaataccca ctcaggaggc gcccggattc ttttataccg
    13621 tatccgcggc gagcccgcgg agctgatcca cttgcgcgtc gttcgcgcgc ggatcaggcg
    13681 aggacatcgc caatcgacgg gtcgcggtcg aattgcgctt tcgcgaacgg acaggtggcc
    13741 atgaccctgg tgccggtgtc gcgggcccat tgcacggcgg catccagcag gcggcggccc
    13801 acgccttttc cgccgaggct cccgtccact tcggtgtggt cgatgatgac cagggtcggg
    13861 ctcgcgcggc tgtaggtcat ttcggccagg cgcttgccgt cctgcatgac gaaaaaggcg
    13921 ccgcgatgac cgtgctcttc gtgcgatacg ttgatatcca tcttcgttcc cgtgctgccg
    13981 gcaggcggct cagaaagcct tgttcctgtc gtcccagatc ttcggctccc agagctcgac
    14041 cttgttgccg tccggatcca tgatccaggc gaacttgccg ttctcgtgcg attcgggccc
    14101 ctggagaatc gcaacgccgg cggcgcgcag ctgggtgagc agttccccga tatcgtcgac
    14161 gcgatagttg atcatgaatg gcgactcgct cggactgaac cattggctgt ccgccgatgc
    14221 gaggcaccac accgtgagcc cctggtcctc ggccttgtct tcgggccatt tcaggatggc
    14281 gccgccgaac tcctccagga cgagccccag gtgcttctgg taccacgccg ccagcgcccc
    14341 cggatcaccc ttgctcttga agaatacgcc gccgatgccc gtgatcctcg ccatgactga
    14401 ctccctgtcg gttttccggc ttcaacggtg cgcgcgcggg atcggatcga gatccggttc
    14461 gacgaagccg gccctggctt cgggcgagaa gagcacgctc gccgaatagt gggtcagcat
    14521 gatccggctg tccagaagct ccggattgct ggcgatgaaa tcggcggccg acgacgccgg
    14581 gcccttgtcc atgaagtgac gcaccgccag cacccaggcg cgagtgagcg tttcgtggta
    14641 cttggtcgcc gggatgccat ggtgttgcag gaaggcgagc agcgccgcgc ggatccgctg
    14701 aacggcctca tcgacatcac ccccgaccag atagacatag gcgaggcgca cgtgggcttc
    14761 gtggtcgaag tcggcgcgaa aggtccgatc cgcatccgat acgtcgtgcg tgctcatgtg
    14821 gcctcctggc cgtgcgcctg cagcgcggct gccatgcgct cgtgaatgag atggatcgcc
    14881 ttgagcggac gaaccatcac cttgaattcc gcgatctcgc cccgcgcatt ccacctgatg
    14941 atgtcggcgc cgttcacgag cgtgtcgtcg atcgtgacct cgaactcgag catggcgtcc
    15001 tgcggtccga taatctgccg cacataatgg aagctgtcgt tgaagaagac gccgaaggcc
    15061 gcggtcaggt agcggagcgt caactcgcgg cccgactgcg ccctgtggac gatcggcgag
    15121 aagaacaccg catctgcggc cagcagctcc ctgagcccct gcaggtcacg gcgctcgagg
    15181 atcccgtgcc atctgtcgac cggatgatcg ctcatcgtgc ccccgcttcc ggctgctgcc
    15241 taggctttcc ggacaaactc cgacttcagc ttcatgggac cgaagccctc gatcctgcag
    15301 tcgatgttgt ggtcctcgtc gacgaggcgg atgttgcgca ccttggtccc gaccttgagc
    15361 ggggcgcccg cccccttgac cttgaggtcc ttgatgacgg tcaccgtatc cccgtccgcg
    15421 agcacgttgc ccaccgcgtc cctgtgcacg ggcggtccct cttcgggcac ggcggcagcg
    15481 cccggtgccc attcatgggc gcattcgggg cagacgagga gttcgccgtc ctggtaggtg
    15541 agcgcggaac cgcaggcggg gcaattgggc agctgtgtca tgtgaagtcc ggggtcgtgg
    15601 cgctatggcg cgagctccga aaggtactcg gagagtccgt cgatgtcatc gacgtcgagc
    15661 ggcgcgagca ccttgatgtg atccggtgcg atgttgggcc tgcgtccgtc caccacgtcg
    15721 tgcaactggc ggcgcaggta catgtactgc tggccggcca ggcgcggcat gagctgtgcg
    15781 tcgctgcctt cggcgcgagg gccgtggcag gcggcgcagc tctgcgcgta cacgcgctcg
    15841 ccacgcgcgc ggatctcggt ctccccgccc ggtccgccgc cggccggcgg catgcgcagg
    15901 ccggcgagat aagccgcgac attcgcgatc tgcgcatcgt cgctgaagcg gtgttcctgc
    15961 atgatgcttt ccatgcgcgc atcccagcgc tcgccttcgc ggtagtcccg cagctgcttc
    16021 acgatgaccg acgcgtgctg cccggcaatc gcgggcacct ggccatcggg cgtgccctgc
    16081 cccgtcatgc cgtggcagcc ggcgcactcg gcaaataccc tggcgccctt cgaggcatcg
    16141 gccacggctc gcgcggcggt gccgggaggc gcgtcgccgc ccggcgcggg ctcgctgatg
    16201 cagcccgcga tcgcggcagc cgcggcaaga gtgatgctca tcgcaaccag tccgtgcatt
    16261 ctcatgcgtc tccccttcac ccgcagtccg cccgatgatg ggcctgcctg gattcgaacc
    16321 agggaccaag ggattatgag tcccctgcac taaccgctgt gctacaggcc cgccgggtag
    16381 cggcacattc taaccgcgac cagggaccaa tacggtggcg acgtgcgaat gccccaagct
    16441 agcatgagct ttttcgagga gccgcccatg tccggagaga taccgccgcg ccccgcctac
    16501 ctgctggttc agggcgtcgt caccgatccc gagggcttca aggcctacaa cgccgcgctg
    16561 ccgccgatct acgcaaagtt cggtggcgcc cacgtgagcc tcacgccggc cgcgaaggtg
    16621 caggtggtcg aaggcgagcc cgagagccgc agcatcgtca tctcgaggtt tccgtcgaag
    16681 gaagccgcgc tcgcgttctg gaactcgccc gagtacacgg cggcaaaggc gctgcgcgcc
    16741 ggcaaggggc atttcttcgt cacggtcctc gagggcctcc cggggacctg acaggcggct
    16801 gcgattcgat cgccgcctcg gcgtcgctcg ggcccgcggg ccaggcgaat gccgcgacca
    16861 gcatcagcag cgcggcagca cccagcagga cgccgccgag gcgactcgcg gccgaccaga
    16921 acagcatcac ggtgctcagc atgggcatga atgcgagcac ggtgaccatc tgcctcgaga
    16981 tgccgcgcgg gtgagccgcc gcgtacagca gcacgatggc gagcagcccg agctgcgcac
    17041 agaaggcgag ccacatcgcg cgcaccgact gctggtagaa gggcttgagg ccgctgttgc
    17101 caaccgcgat cgaggtcgcg acataagcga acgcgaaata gaccgcgaca ccgccgatga
    17161 tcagcgccgc accgaccatg caccagcgga atacgcgccg tccgttcatg tcagcctccg
    17221 ttgcacgaag cgcgagatcg atgtgtacgc gccccagagc gcaaatgcca tcgagaccac
    17281 gacggtaatg ccgaccgcca cagtcttgcc gtacttcgcc agcagcgcga tggtcgcgat
    17341 accgccgtgc agcggggggt agcggccgat gacccaggcg ttggcgacat tctcgatcca
    17401 gtcgcacacg cagccgacga agcaccacgg cacccagcgc cgccaggcgc ccgacgcgag
    17461 cagatcgggg cgcgcgcgcc gcagacccca gaccgccagc gccgcccaga agagcgcggt
    17521 caggaacgga aacgggaaat cgatgcccga atagcgacca tagaggcgca ccgcctcggg
    17581 cgtgtagtgg gtgagctgcg cggcgatgtc ggcgagcgca agcgtgccct gcaggtcgaa
    17641 gacgtcgtgg ccggtgagca ggcgcatccg ggcgctgtac tcgagcaggc gccgcgccat
    17701 gaagagaatc gccagcgcga gcagcccgag cgcgagccag cgtctcgaca gagcctcgac
    17761 cagccgtgac agggtcacca cgccgctagc atgccccaag tgcggcttcg gctacagcgc
    17821 gaccccgccg accagtcggc tcgtcgcacc cgccgatcgc atcggcgcag ccggtattcg
    17881 cttcggcgcc gcgggcgcgc atgatcgctc cgctgtcgcg aaagggacac catgcgcacc
    17941 accatcctgc ttctggccac attgccgctc gtcgtaccgg tcgccctggc caccgatgct
    18001 gccgcggatt cgatctactc gcgcgccgtg gccgacccgg cccgccccgc gaaggaccgc
    18061 gagcgcgacg cgcgcgacaa gcccgccgaa gtgctcgcgc tcgccggctt cgggcccggc
    18121 atgaaggtcg ccgacatctt tgccggtggc ggctactact ccgaactgct gggcgacgtg
    18181 gtcggccccg gcggcagcgt gctgatgctg aacaacaccg cctaccagca cttcgcgcgc
    18241 gaggagctga aggagcggct caaggacggg cgcctcgcga acgtcaagcc gatgctgatc
    18301 gaaagctgcg acctgcgcct cggcaacgac gatctcgacg gcgcgctgat cgtgatgtcg
    18361 tatcacgacc tctactacgc ggacgaagcc ggcggctggg caccgatcaa cgccggcagg
    18421 ttcctcgacc agatccacgc ggcgctgaag ccgggcggcg tgttcctgat cgtcgatcac
    18481 gccgcggccc gcggcaccgg ccacagcgct gcgcaggaac tgcatcgcat cgacgaggca
    18541 ttcgcgaagc tcgacatcga gagtcacggc ttcaggctgg agaagacctg ggatggcctg
    18601 cgcaacgagg ccgaccggca cgatgtgggc gtgttcgacc cgaaggtgcg cggcagcacc
    18661 gaccgcttcg tgcacgtgta ccgccgcatc tagcggcggc gcttcacgcc gcgcgtgggc
    18721 tccgcctcga gcgggttctc gggccagtag tgcctcggat agcggccgcg cagatccttg
    18781 cgcacctcgg cgtaaccgct gcgccagaag ctctcgagat cgcgcgtcac ctgcaccggc
    18841 cggcgcgccg gagacagaag tgtgaaggtc acgggcagcg cgccgtccgc gatgcgcggc
    18901 gtcgccgcga gcccgaacac ttcctgcagc cgcaccgcga ccgtcggtga ggcgggatcg
    18961 gtgtagtcca ccggcagcgt ggaacccgtc ggcatcgtca ggtgtgtcgg cgcgagccgg
    19021 gagagtgccg cgcgctgcgc gtgcgtgagc tgcgcgccga ggatctcgct catcggcagg
    19081 cgcgcaaggt gcgcgcgccg cgtgatgccc ccgagccagg gcgcgagcca ttccccgaga
    19141 tgcgccgcga gcgcggcgtc gccgagatcc ggccactcgg cgggatccgg cacgtgcgcg
    19201 cgaacgaatg ccacgcgctg ctgcagaccg cgggcggcgg cggtccaggg caacgcatcg
    19261 agtccgagcg acaccactcc cgccagcatc gccgcctcgg cggcaccggc aggcagctcg
    19321 cgcagcgcac gctcgtcgag agtgagcgcg ccgagcgtgc gcaggcgcct ggcgatgacc
    19381 gcctcggtgc gcgcatccca ctcgaccaca tcgcgcgtct cgatcgcgtc agtgaacagc
    19441 gcctcgagcg ccgcgcgcga caacggggcc gcgagccgta tgcgcgcgtc gcgggcggca
    19501 tcgtcgatcg ccgcgatggc gatgaactcg ctgcccgaga ccgtgtccgg tcgcgtgaac
    19561 tgcgcgccgc gtccgttcgc gagcgcatag cggccctcgc cctccccgcg ccggcgcccg
    19621 atccggtcgg gataggcgag cgccagcagc tcaccgggtt cgacgtcgga ggaatcgccg
    19681 gcgccacccg cagcaccggc atgtccgccg gcgccgatct gcgtgcgcaa ttgctgcgca
    19741 gcgcggcgca cgcgctcgag cgcgccgcga tccacatcct gcgcgccgtg cccccgatcg
    19801 cgcagcgcct ccagtcgcgt gcgcaggtcc gcatcgcggc ccgcgccgcg cagcaggtca
    19861 cgctcgccga gcagcgccgc gatgtcggcg gcgagcgccg tcgcctgcag tccccgcgag
    19921 cgcagcaaca tgtgcgcgag gcgcggatgc acgggcagat cggccatcgc gcggccatgg
    19981 gcggtaacgc gatgctcgcg atcgagcgca ccgagccagc gcagcaggtc gcgtgcgctc
    20041 gccagctggg ccgcgggcgg cgggtcgagc catgccagct gtgcggcgtc ctgcgcgccc
    20101 cacacggcga gctcgagcgc gagcggcgcg aggtcggcct cgaggatctc cgcgggtgtg
    20161 tacgcggcga ggctgcggtg tgcgccctcg ccccacagcc ggtagcacac gcccggcgcg
    20221 gtgcgcccgg cacggccctg ccgctgatcg gcggaggcgc gcgagatgcg tcgcgtctcg
    20281 agacgcccca tgccgctcgc ggggtcgaag atcgcgcggc gcacgaggcc gctgtcgacg
    20341 acgacgcgca cgcccggaat cgtgaggctc gtctcggcca ggttggtggc gagcacgatc
    20401 cggcggcgcc cgtcacccgc gggggccagt gccgcgagct gcgcctcgcc tgtcagctcg
    20461 ccatagagtg cgcggatgtc gacatcggcg ccggtcgacg cctcgagcgt ggcggcgacg
    20521 cggcgtatct ccccggctcc cggcaggaag accaggatat cgcccgtctc ctcgcgcagc
    20581 gcacgcagta ccgcaagcga cgtgagccgc tccggggagt ccgcctcgcc aggcagcacc
    20641 ggcaggccgc tgcgcaggta ccgggtctcg accgcgaact ggcgcccctg cgcggtgacg
    20701 atcggagccg ctccgatcag cttcgcgacg gcctcgccgt cgagcgtcgc cgacatcacg
    20761 acgatgcgca ggtccgcccg cagcgcggcg cgcgcctcga gcgtgagcgc gagcccgagg
    20821 tccgcgtgca ggctgcgctc gtggaactcg tcgaacagta cggcggccgt gtcctcgagt
    20881 gccggatcgt gctgcaggcg ccgcgtcaac acgccctcgg tgatcacctc gatgcgcgtt
    20941 gcgcgcgaca cgcgcgtttc gaggcgcatg cgatagccga cctgctcacc cacccgctcg
    21001 ccgagcgagg cggccatgcg ctcggcgacg gcgcgggcgg cgagccgccg cggctcgagg
    21061 accagcagct tgcgtccggc gagccatggc tcggcgagca acgccagtgg caccaccgtg
    21121 ctcttgcccg cgcccggggg cgcctgcagg atcgcgctgc gctgctctcg cagcgcggcg
    21181 cgcagcgcgg gaagcacctc ggtgaccggc agctctgcag gcggggcata tgacacacgg
    21241 tcattctatc gagcgccatg cggtatgctc tctgccatcg ccaggggaga ctgcaatgcg
    21301 ccttggacgc agccgtcgca gcaccaacat cgaagaccgc cgcgcgagcc ccggtggcgg
    21361 caccggcatg aagttcggca tcggcacgct cgtggtgctg ctgatcggtt atttcatggg
    21421 catcaatccg gccacgctcc tcgggctcgt gggaggcctc gagggcgcag gccccgccgc
    21481 gccgcccgtc gtcggcacgc ccaccgacgc ggaagccgac ctcatcgcgg cggtgctcgc
    21541 cgaaaccgag gacacctggg gcggcatctt ccgccagggc ggctcgcagt acctgcctcc
    21601 caccctggtg ctgtttcgcc agtcggtcaa ttcggcctgc ggcttcgcga cctccgcggc
    21661 cgggccgttc tattgtccgg gcgaccgcaa ggtctatctc gacctcgcct tctacgacga
    21721 gctcgcgacc cggttccagg cgcccggcga tttcgcgcag gcctacgtac tcgcgcacga
    21781 agtcggtcac cacgtgcaga acctcgtcgg cacgctcggc caggtgcagt cggcgaagca
    21841 gcgcctcagc gaaggcgagc agaaccagct gcaggtgcgc gtggaactgc aggccgactg
    21901 ctatgccggc atctgggcgc actatgccga aagatcgagc ggcatcctcg agccgggcga
    21961 catcgacgag ggcctgcggg cggcatcagc tgtcggcgac gacacgatcc agcgccgcat
    22021 gcagggtcat gtggtgcccg aatcgttcac gcacggttcg gccgagcagc gcatgagctg
    22081 gttcatgcgc ggcttcgaga gcggcagact cgaatcctgc gataccttcg cagcgcgctg
    22141 aacggatcgt cagcgcgcgg accagacggt cagtgcgacc agaatccagc cgagcatcag
    22201 cagcaggccg ccgagcggcg tcaccatgcc gagcgccaag ggcgcgccga acgtgagcgc
    22261 gtagatgctg cccgagaaaa ccacgatgcc ggcgacgatc agtttcgtgg cgagccgcag
    22321 cccgcgcgcg ggacgcagct gcgccagcac gccgagcgcg agcagcccga gcgtgtgata
    22381 gaagtggtag cgcactccgg tctcgaacac gtcgaggcgt tccggcgtga gcacgtcccg
    22441 caggccgtgg gcgccgaatg cgccgagcgc cgtggccagc gcgacgagca gcgctgcgat
    22501 ggcgagggtc cggcgggcgt cgttgttcat gcttgctgct ccgaagtcac ggcgtaccga
    22561 acacgtcgag cggtatgcgc aggtaagcga tgccgttggc ctgcggcgca ggcagtgcgc
    22621 cggcgcggat attcacctgc agcgccggaa tgatcaatgc cggcaacgcg agctcgcggt
    22681 cgcgcgcagt acgcagcgcg acgaattctt cgcggctcgt gccggcgcgc agatggatat
    22741 tgctgctgcg ctgttcgccc accgtggttg cgaactgcgg cgcctcgccc ggctgcggat
    22801 agacgtggca catgaagagc cgcgtcgcct cgggcagtgc gtaaagcagc ccgatggagt
    22861 cgtacagccg tcccgcgtcg ccgccgggaa agtcggtgcg cgcagtgccg gcctgcggca
    22921 ggaacagcgt gtcgccgatg aaagccgcat cgcccaccag gtaggcgagg ctgtcgatgg
    22981 tgtgccccgg tactgcgatc accctcgctt cgagtgtgcc gatggcgaag cactcgccgt
    23041 cggcgaacag atgatcgaac tgcgaaccgt ccaccgggca ttcgggcccg aagccgaaca
    23101 gcggcgcgaa ctcgcacagc acgtggcgga tgccggcacc gatcgcgatt cgcccgccgg
    23161 cctgcgccct gaggtaggcc gccgccgtca ggtgatcggc gtgcgcatgc gtctcgcaga
    23221 tccactcgag cgtatcgccg cgcgcgcgca atgccgcgag cagcgcatcg gccgagcgtg
    23281 tcgcggtgcg gccggacttc gcgtcgaaat cgagtaccgg atcgatgatc gctgcccggc
    23341 gcgtggcagg atccgaaacg atgtaactgc aggtgccggt cgccgcatcg tggaaggccg
    23401 tgatggcggg cgcgccgacg ctcatctgcc acgcggctgg ttccgcggcg ccggcttggc
    23461 ccgtgcgggc ctcggccgcg tgtgctgggt gcgaaagggt cgggtgttcg ccgccgcgcg
    23521 ccgtccctcg cccgccgtgc cggtgccggc gggctgccat gcgggcacca gctggtgcct
    23581 gccgttgccg atgagatccg cgcgccccat gcggcgcagc gcctcgcgca gtaccggcca
    23641 gttgttggcg tcgtggtaac gcaggaaggc cttgtgcagc cgtcgaacct tgagaccctt
    23701 cggcaccacg accttctccg agctgcgcgt cacccgatgc agcggattcc tgccgctgtg
    23761 gtacatcgcg gttgccgtcg ccatcggcga gggcaggaac gcctgcacct ggtcggcgcg
    23821 aaaaccgttc ttcttgagcc atagcgccag ctcgagcatg tcctcgtcgg tcgtgcccgg
    23881 atgcgcggcg atgaagtacg ggatcaggta ctgctccttg cccgcctcct tcgaatagcg
    23941 ctcgaacagc tccttgaagc ggtcgtaggt cgcgattccc ggcttcatca ttttcgacag
    24001 cggcccttcg ctgatcgcct cgggcgcgat cttgaggtag ccgccgacgt ggtgctggac
    24061 cagttccttc acgtactcgg gcgattcgat cgcgaggtcg tagcgcacgc ccgaggcgat
    24121 cagtaccttc ttgacgcccg gcacttcgcg cgcgcgccgg tacaggccga tcagcgccga
    24181 gtggtcggta tcgaggttcg ggcagatgcc gggatacacg caggacggac gccggcacgc
    24241 cgcctcgatc tcgcggctcc tgcaggcgat gcgatacatg ttggcggtcg gcccaccgag
    24301 gtccgagatc acgccggtga agcctggcac ggtgtcacgc acctgcccga tctcgcggat
    24361 caccgaatcc tccgagcggt tctggatgat gcgaccctcg tgctcggtga tcgagcagaa
    24421 cgtgcagcca ccgaagcagc cgcgctggat cgagatcgag aagcggatca tcttgtacgc
    24481 gggaatctcg gcgtcgccgt aggtcgggtg cggccggcgc tgatacgggc gctcgtacac
    24541 ccagtccatt tctttcgtgg tgagcgggat cggcggcgga ttcagccaca cgtcgacgtc
    24601 gccatggcgc tgcaccagcg cgcgcgcatt gcccggattg gactcggtgt gcaggatgcg
    24661 cgaggcgtgc gcgtacaggt cggaatcggc ggcgacctgc tcgaacgacg gcatcctgat
    24721 gaccgtatgg gcccggtcgg cccggcgcac gccaggcgac gcggcagcgc cttcccggcg
    24781 cagctcgagt gtcgtcgcgt cgatttccgt ccagccctgc ggcatgcccc ggcgcatgaa
    24841 cgcggtgcca cgcaggtcgg tgagcgcatc gatcgactcg cccgcggcga ggcggtgcgc
    24901 gatctcgcag atctggcgct cgccgttgcc gtagaccagc aggtcggcct tggcgtcgag
    24961 caggatcgaa cggcgtacct tttccgacca gtagtcgaag tgcgcgatgc ggcgcagtga
    25021 tgcctcgatg ccgccgatca cgatcggcac gtcgcggaac ccctcgcgta cgcgctgcgc
    25081 atagacgatc accgaacgat ccggccgcct gccgcccgcg ccattcggcg tgtacgcgtc
    25141 atcgctgcgc acccgccggt cggaggtgta gcggttgacc atcgaatcca tgttgccggc
    25201 ggtgacgccg aagaacaggt tcgggcggcc gagcgccttg aacggttcgg catcgtgcca
    25261 gtcgggctgg gcgatgatgc cgacccggaa gccctgcgct tcgagcgtgc gcccgacgat
    25321 cgccatgccg aagctcgggt gatcgacgta cgcgtcaccg gtgaccagga tcacgtcgca
    25381 cgaatcccaa ccgagcgcct ccatctccgc gcgcgacatc ggcaggtacg gagccggccc
    25441 gaagcgcgcg gcccagtacc tgcgatacgc cgtgatatcg cgcgccggct ccatctcagt
    25501 ccttgccgcc cgccgcctcg agcgcgcccg cgaggcctgg aacaaacgcc gcgccttccg
    25561 gcacctgcac gctcgacccg acgaagtcgt cgccgaccgc ctcgtagcgc ccgccgaggc
    25621 acttggcgag gaagccctcg gagatcgcat acgccgaagt gcgattctgc ggccgcgcga
    25681 agccgtggcc ctcgtccggg aacagcacat aggtgaccgg caggcccttg gccttcatcg
    25741 ccgccacgat ctggtcggac tcggcctgct tgacgcgcgg atcgttcgcg ccctgcatga
    25801 tcagcaacgg cttcgcgatc gcggaaacgc gggtcagcgg cgagcgctcc tcgagcagtt
    25861 tcctgccctc gggcgtgcgc ggatcgccga ctcgctgcgc gagttgctcg aagaacgcct
    25921 tccagtacgg cggaatcgag gcgagcagcg tcgcgaggtt cgagggtccg acaatgtcga
    25981 cgccgcaagc gaagcgatcg ggcgtgaagg tgaggcccac gagggtcgcg taaccgccat
    26041 aggaaccgcc gtagatcgcg acgctgtcgg cattggtgat cttctcgcgc acggcgaagt
    26101 cgaccgcgtc gatcaggtca tcatgcatct tcgcggccca ctcgaggttg cccgcgttga
    26161 cgaacttctt gccgaaaccg accgagccgc gatagttcac cgacagcacc gcatagccgc
    26221 ggttcgcgag ccactggtgc tcgggatcga agccgtacgc gtcgcgcgcc caggggccgc
    26281 cgtgcacgtt cagcacgagc ggcagcggcg cctcgggacg cccgttgccg tccgcgtcgc
    26341 tgcccttcgg cagcgtgagg taggacacga gcgtaaggcc gtcgcgcgac ctgatttcga
    26401 gcggtgtcat cggcgcgagc ggcgcaccga ccagcgccgg gcgctggtcg aacagcttca
    26461 ccacgctgcc gctgtcacgg tcgtacaggt ggctcgaaag cgcgctgacc ggatcgtcga
    26521 ccaccacgat ccacttgcgg tcatcgagcg tgcggctcac gacgtcgaac tgtggaccga
    26581 gcgtcttcgt cagcagatcg atgtccttct gcacgagcgg cgtgagcgcg gtgagcgtgt
    26641 cgacgagata gttcgccgac caggcctgcg gcttgcgcga cagcggctcg atccacacgc
    26701 cggcgacgtc ggccctgtcg ctcgcggcga tcacggtctg cgcgccgctg ccgaggtcga
    26761 cgcgcacgag cgccgcggta tcgcggccca ccgaggagcg gatcagcgcg gtcgtgccct
    26821 ccccttcgac gatcagcggg ccggtggttg cggtgtcctc ggggccgtac ttcatcaggc
    26881 tgtcccagcc gcccttggcg ttgcgcacga acatctcgtc gtattcggcg ccgggcttca
    26941 cggcgatgcg cggctggagg ctggcatcct cgagataacc accgaactcc tggtcgttct
    27001 gctcgacgag cttgcgctca ccggtcgcga tctcgatctc gtacaggtcg tgccattcac
    27061 gcttgcggtc gttcagcccc acgagcacca gaccgggccg ccgctcgctg acgccgatca
    27121 cctgcgcctg tacgccgtcg atcggcgtga ggtcgatgtc attgccggtg gcgatgtcta
    27181 cgctgtgcac gcgccagttc tcgtcgccgc cctggtcctg caggtacaac acgtgcttgt
    27241 tgtcgtagga ccagaagtgc tggcggatgc cgcgcttcgt gtcccgggtg acgggccttg
    27301 cctcggccag cttgccgaaa ggcgccaccc agacgttcag cacgccatcg cgcggcgcga
    27361 tgtacgagat gtgcttgccg tcaggcgaca ggcgcgcctg ggtctgggtg ggattgccga
    27421 acagggccga gcgcgggatc agcgggccgg gctcggcggc gacggcggaa acgaatgatg
    27481 acgacatcaa caaggctcct gcgagcgcca gaggcgaaca cttggacatg gaattcccca
    27541 gttcttgcgg gccgggcccg cgactttgag ggagggaatt atatgccctg caggggagcc
    27601 acgaccggat tgtaggagaa gaggccgacc gactcggcgg tggtatgcgg gtgagtcgcc
    27661 cggtcttcgg cgtcggcacg gtggtattcg aggccggttt cgcgggattt gcccattacg
    27721 aacgtggtgc gcccgatccg tgtggcctcg tacaggcgca gcgcctcgtc atggctcccc
    27781 gcgagctcga acgcgcgcgc gagcaccatg gcgtcctcga tgcccatcgc cgcgccctgg
    27841 ccgaggaacg gcagcatcgg gtgcgcggca tcgccgagca gcgtgacgcg gccgcgtgtc
    27901 cagctcgcca gcggttcgcg gtcgaacagc gcccacttga agcacaactc gggtggcgtc
    27961 gccgcaatga tcgtgcgtac ttccgggtgc caagccttga actcggcgag caactcgccg
    28021 acctccgagc gcaccgacca gctctcgacc tgccagtcgc tgcgctcggc caacgccacg
    28081 taattgagga cgcgcccggc gcgaatcagg tagcgcgcga atgtgtgctg cggcccgatc
    28141 gaaacgcagg acgccggatc gaccagtccc ggcggcaatg cgtcgatcgg cacgaggccg
    28201 cgccaggcga tgtagccggt gaagcgcggc tgcccggcac cgaacagttg ggcgcgcacc
    28261 gcggagcgga tgccgtcggc gccgatggcg acatcggcct cggcccgcgc accgttcgcg
    28321 aagcggatcg tgacgccgtc ggcgtcctgg ctcaggctct cgaagcgatg gtcgagcagc
    28381 aggcactgcg gatcgtgcgc cagcacgctg cgcgccagag cgtcgtgcag gtcggcgcga
    28441 tggatctgca gatacggcgc accgtagcgc tcagtgaggc ccgcgccgcg ctcgaattcg
    28501 gccagcagtc tgcccgtgcg ccagtgccgc gcgccgccgc gcgcgggctg catgccgtgg
    28561 cgcgcgagct catcgcccaa gccgagcgcg agcaggacat gggtcgcatt gggtgtgacc
    28621 gtgagccccg cgcccacctc ggcgagccgc tcggcctgct cgtacacgcg cacgcgaaag
    28681 ccgcgctgct gaagcgcaca ggctgcgctc aagcccgcaa tgcccccgcc gaccagtgcg
    28741 atccgcaagt ttctcatgca cacatgatgc cgaaccggca cgccggagca cgcgccgcgg
    28801 caccgccgtc cgcgccgcgg gcaaccgctt gacggcgcgt gtagacttgg gggtttgcct
    28861 ccaccgccac cacaaccact gcaggcaacc atggcgaaga ctgggcccgg cgtcatcgcc
    28921 gtccacggcg cgcgccagaa caacctgaag aacctcgacc tcgagatccc gctcggcgag
    28981 ctcgtcgtcg tgacaggcgt ttccggctcc ggcaagtcct cgctcgtgtt cgacacgctg
    29041 tatgccgagg gccagcgccg ctacgtcgag actttctcgc catacgcgcg ccagttcctc
    29101 gaccgcatgg acaagccaca ggtggagcgc gtcgagggca tcccacccgc gatcgcgatc
    29161 gaccagacga accccgtgcg cagctcgcgc tccaccgtcg ggacgatgac cgagctgaac
    29221 gatcacctga agctgctgta tgcgcgcgcc gccacgctgt actgcgaacg ctgcgccgag
    29281 ccggtgcgtc gcgacacgcc cgacacgatc tacgccgaca tcgaacggcg cgccgcggcc
    29341 gccgccgatc cgcgcctggt gctgactttc gcggtaccgg tcccgaagaa tttcgcggag
    29401 gccgaagtac gcgccctgct cgcgcgccag ggctatacgc gcatcctcga tcgcgcgggc
    29461 gccggcgcgc cgctcgaggt cgtgcaggac cgtttcctgg tgagtcaggc cgagcgcgcg
    29521 cgcgtgatcg acagcctgga ggcggcgctg cgccatggca ccgggcgcat cacggtgcgc
    29581 acgccggacg acacctggca ctactcgcga gaactcgcgt gcgcgcactg cgacatcgcc
    29641 tgtcgcgaac ccgcgccgag catgttctcg ttcaactcgc cgctcggcgc ctgcgagacg
    29701 tgccgcggct tcggccgcgt gatcggcgtc gactacgggc tcgtgatccc ggatgagacg
    29761 aagaccctgc gtggcggggc gatcaagccc ttccagaccg aatcgaaccg cgagtgccag
    29821 gaagacctcg agaagtacgc gcagctgcgc ggcattccgc tcgacacgcc gtggcacagt
    29881 ctcacacccg ctcagcgcag ctgggtgatc gagggcgaag gcagctggcg caagaaggtc
    29941 tggtatggcg tcaggcggtt cttcgcctgg ctcgaaagcc gcgcctaccg gatgcacgtg
    30001 cgggtcctgc tctcgaagta ccgcgcctac acaccctgcg aggcctgcgg cggcgcgcgg
    30061 ctgaagctgc cggcgctgct cgcccgcatc ggcagtcacg aactggccga tgcggcactg
    30121 ggtaccggcg ggccggccgc acgcacgcgt ttcatgccgc atggcgtcgc atggagcgac
    30181 gcgcagctcg aagcgctacc cggcctgtcg atccacgacc tgatgctgct gccgctcgag
    30241 cgggtgcgcg agttcatcga cgcgctcgtg ctgcccggtg cgctcgatgt cgccaccgac
    30301 cagctgctcg gcgagatccg cacgcgcatg cgctatctcg tcgacgtggg actcggctac
    30361 ctgacgctcg accggcaatc gcgcacgctc tcgggcggcg aagtccagcg catcaacctg
    30421 acgacggcgc tcggcacctc gctcgtcaat acgctgttcg tgcttgatga gccctcgatt
    30481 ggcctgcacc cgcgcgacat gggacgcgtg atcgacgtga tgcaccgcct gcgcgatgcc
    30541 ggcaattcgc tgctggtcgt cgagcacgat ccgcagatca tgctcgaggc cgatcggctg
    30601 ctcgatctcg gcccgggcgc cggcgaacgc ggcggcgaga tcgtctacta cggcacgccg
    30661 gaaggcattc gcagcgcagc cgggtcgctg accggcgcgt acctttccgg ggagcgcacc
    30721 gtgtccgagc cgcagcgcac ggctcccgcc gccgcgcccg tcgccatgcc cggcgtcgcg
    30781 catgccgtgc gtgcgagcgg ctcgctggtg ctgcgcgggg cgcgcgagca caacctgaag
    30841 aacatcgacg tcacgatccc gctgcgccgc ttcgtgtgca tcaccggtgt gtcgggatcg
    30901 ggcaagtcga cgctggttgg aaacgtgctc catccggctc tcgcgaaggc cttcggcaag
    30961 gcggccgatg cgcccggaac gttcgagagc ctcgaaggcg cgggctacct cgatgacgtc
    31021 gtactggtcg accagagccg catcggccgc accacccgct ccacgcctgc aagctacgtc
    31081 ggtgccctcg acgtgatccg cgagctgttc gccgccgcgc ccgaatcact cgtgcgccgc
    31141 tacacacccg gcaccttcag cttcaactcc ggcacgggcc gctgccccac ctgcggcggc
    31201 aacggcttcg agcacatcga aatgcagttc ctctccgacg tgtacctccg ctgtgcgagc
    31261 tgcgacggca agcgcttccg ggacgaggtg ctcgaggtgc ggctcgaggg cgcacacgga
    31321 cggcacgcga acatcgccga cgtgctcgac atgaccgtga ccgaggcgct cgagttcttc
    31381 gcacaggcga gcgcggtgcg cgcgctggtg aagccgctgc aggaggtcgg cctcgagtac
    31441 gtgaagctcg gccagccggt gcccaccctg tcgggcggcg aggcgcagcg cctgaagctc
    31501 gccgggcatc tcgctgaact ggtttcgccc gcgccgaagc gcgccgccga ggtgcgcacg
    31561 cgcggcaaac tcttcctgtt cgacgagcct acgaccgggc tgcacttcga tgacgtcgcg
    31621 aaactgctgc gcgcactgcg cgggctgctg tcggcgggcc actcgctgct cgtaatcgag
    31681 cacaacctcg acgtgatccg cgccgccgac tggatcatcg atctcggccc ggacggcggc
    31741 gatcgcggtg gcgaggtcgt ctgcagcggc acaccacagg aggtgcgcac gcatgcgcgt
    31801 tcgcacacgg cgcgtgcgct gcgggactac gaggcggcgc tcggccgccc ggtgctcgca
    31861 gtcgccgaca ctcccgcacg cgcgccccgc ccgcgacgcg acaacgcgat cgtcgtgcac
    31921 aatgcgcgcg agcacaacct gcagaacgtg cacgtgcgca tcccgcgcga cgccttcacc
    31981 gtggtgaccg gcgtgtccgg ctcgggcaag tccacgctgg ctttcgacat cctgttcaac
    32041 gaaggccagc gccgttacct cgaatcgctg aatgcctacg cgcgccagtt cgtgcagccg
    32101 gccgcccggc ccgatgtcga tgcgatctac ggcattccgc ccacggttgc gatcgagcag
    32161 cgaaccagcc gcggcggacg caagagcacc gtcgcaacgc tcaccgagat ctaccacttc
    32221 ctgcgcctcg cgtgggtgaa gctcggcaca cagtactgcc cggactgcga cctcgcgatc
    32281 aagccgcagg gtgccgacgc gctcgccgcc acgatcgccc gcgaatatcg cggccagcgg
    32341 gtgcgcgtgc tcgccccgct cgtcgtgtcg cgcaagggct attacacgga tctcgcgaag
    32401 tgggcggcaa agaagggctt tcgcgagctg cgcgtcgatg gcatcgatac gccgacggcg
    32461 cgctggccgc gcctgtcgcg cttcaaggaa cacacgatcg aactgccgat cggcgacctg
    32521 cgggtcgagc ccgcgcgcaa cgcccagctg cgcgccgccc tcgatcgcgc gctggagtac
    32581 ggcaagggcc tcgtgcacct gctcgacctc gatgcgcggc gcgccggcaa ggtggcggtg
    32641 ttctcgaccc ggcgcgcctg ccccggctgc ggtgcgagct tcgccgagct cgatccgcgc
    32701 ctgttctcgt tcaattcgcc gcacggctgg tgcccgcact gccagggcgc gggcaccgaa
    32761 ccgggtgacg acggcgacga gttcgcagac gaggatcgcc cggtgtgcag cgagtgcgac
    32821 ggcgagcgac tgaaccgcat cgcgcgccac gtgcgtctgc gcgatcgctc gctgccggcc
    32881 ctcacccgcg atgccgtcga tgctctcgcg caatacttca ggaccctgcg cctggcggga
    32941 cgcgaggccg cgatcgcgcg cgacctgctc accgagatcc gcgaccggct ggccttcctg
    33001 cagcgcgtgg gcctcggcta tctcgcgctc gatcgcgcgg cgcccaccct ctcgggcggc
    33061 gaggcgcagc gcatccgcct cgccgcgcag ctcggctcga acctgcaggg cgtgtgctac
    33121 gtgctcgatg agcccaccat cggcctgcat ccgcgcgaca acgcggtgct gctcgccgcg
    33181 ctcgatgagc tggtcacgca ccgcaacacg ctcgtcgtcg tcgagcacga cgaggagacg
    33241 atcctgcgcg ccgatcacgt catcgacctg gggcccggcg ccggcacgcg cggcggtcgc
    33301 atcgtcgcag agggtcgcgc gcgcgatctc gagcgctcgc ccgaatcggt caccggccgc
    33361 tatctcgcga acccgctgca ccacccggcg cgcccgcgtc gcgcgaccag cacccgctcg
    33421 cccgcgctcg cgatcgagcg cgccacgctg cacaacctgc agggcgtgag cgtacgcgtg
    33481 cccctcgggc gcctggtcgt ggtgaccggc gtgtcggggt ccggcaagtc gacgctcgcg
    33541 cgcgacgtcc tgcacgccaa tctgcaacgc atcgtcgccg gcggacgcac gcgatcacgc
    33601 gcccgtgcga cactcacggg ctgcggggcg attcgcggca gcgaggcgat cggccgcgtg
    33661 ctcgaagtcg accagacgcc gatcggcaag acgccgcgct cgtgtcccgc cacttacgtc
    33721 gggttctggg acgatatccg ccgcatctac gcggcggcgc ccgagtcgaa gctgcgcggc
    33781 tactcggcga gccgcttctc cttcaacacg gcgggtgggc gctgcgaggc ctgcgaaggc
    33841 cagggctcgc agaccatcga gatgagcttt ctgcccgatg tgaaggtgcc ctgtgacgtg
    33901 tgccgcgggc agcgcttcaa tccggagacg ctgtcggtca cctggcgcgg gcgaagcatc
    33961 ggcgacgtac tcgcgatgag catcgacgcg gcggtggagt tcttcagcgc gcacgcgaag
    34021 gtacaccatg ccctgtcgct gatgcaggac gtcgggctcg gctacctcac gctcggccag
    34081 cagagcccga cgctctcggg cggcgaggcg cagcgcatca agctggtcac cgaactcgcc
    34141 aaggcacgca cttctgcggg cgcgcggctg cgcgcccagc cggtgcacac actctatgtg
    34201 ctggacgagc ctacggtggg actgcacatg gcggacgtgg agaagctgat cgcgacgctg
    34261 caccgcctgg tcgatgcggg caacaccgtg gtgctcgtcg aacacaatct cgacgtgatc
    34321 gccgaagccg actgggtgat cgatctcggg cccgaggccg ggactgcggg cgggcgcatc
    34381 gtcgcgcaag gcccgccggc acggatcgcc cgcgcgaaga cctcgcacac cggcgctgcg
    34441 ctcgcgccgt tcctggccga gcgcaccgcc tgaacggaaa cggttcagct cagccgcgcc
    34501 actggcggct gcgcacattc tgcagctgcc aaggccggtc gatatcgagg cgctcctcga
    34561 aaagttcgcc gcgaccctgc agcggcgtcc agttggtgaa ctcgccggtc atcctgccga
    34621 gatagggtat ggccgtatcg aggcagtcct cgaaatcgag gtcgtccggt tcgacgatgc
    34681 cgcgccgggg attgcgcatc gcccacacga ttgccgcgag cgcgccggcg gcgacctgca
    34741 gcgtggtcgc gttggcgaac ggcacgtggc ggcgcgcctc ctcgatcgtg agctgcgagc
    34801 cataccagta ggcgttgcgg ctgtgcccgg cgagcaatac gccgagttcg tccatgccgg
    34861 tcacgatgtc ggcaccgagc cgccgcttct tctcctgcgg ctgccagccg cgcccctcga
    34921 gctcgagcac cgagagcatc gcgtcgtcgc agggatggta gacgaacatc
//
LOCUS       DAOQVY010000006        34857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_1421_length_34857_cov_6.249670, whole genome shotgun sequence.
ACCESSION   DAOQVY010000006 DAOQVY010000000
VERSION     DAOQVY010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 34857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34857
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1421_length_34857_cov_6.249670"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PKN91_01315"
     CDS             <1..196
                     /locus_tag="PKN91_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNS26737.1"
                     /translation="IEAQAREHGLSTERVISEIMLKPMPKKAFVTVEEIAALAAYLIS
                     DAARNVTAQAIAIDGGWTAQ"
     gene            complement(222..1130)
                     /locus_tag="PKN91_01320"
     CDS             complement(222..1130)
                     /locus_tag="PKN91_01320"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNS26738.1"
                     /translation="MDEHGAPHGEAPVAVFVADDFAACNERAFVLTAVGIPFQIARAP
                     AGHALLVDPAQARAAREHLARYAAERLAVRAASPPRRIAHPFAWFGSLLYVVVLLAVT
                     LAVGAGLGPLDAYARGVADAGLIRAGEWWRAITALTLHVDAPHLVANIGGGAWFSYLA
                     GRRLGPGAAWALIVLAAMAANLIQGLLGPVPYRSVGASTAVFAALGLLAAHSWREHFP
                     LRARWAVRWAPLVGGVLLLGWLGSSGEDTNVMAHILGFSCGAAAGLAIARSRVEAALA
                     RVPQWVGGALAIGLVVLAWSLALARG"
     gene            complement(1130..2488)
                     /locus_tag="PKN91_01325"
     CDS             complement(1130..2488)
                     /locus_tag="PKN91_01325"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNS26739.1"
                     /translation="MSAAHILVVDDESDIRGLLTEILTEEGYEVEAAANAAEARAARA
                     NRAPDLVLLDIWMPDVDGITLLREWSAQGGESCPVVMMSGHGTVETAVEATRLGAFDF
                     VEKPLSLAKLLRTVERALDAGRRRRGGRLTVPPLMVPVGKSSLMRQLRTELAQLANSQ
                     RVLFIGESGSGREAFARYLHEAGPDASQPVVTLVASTVSSSDPEPLLLGSESGGVVKP
                     GVLEQAAGGTLFISEIDDLPASAQRILLGVLETMHFTRVGGTEPVAFNARVFASAQPG
                     IEQRAADGSFRRDLLSQLNTLIFLVPPLREYAEDVPELLRYYVDRLVDAEGLRFRRFS
                     VAAQNRLRNYPWPGNIREMHNLVHRQLLRGGPEEIELEEVERELAVRTPQEEPLVKQD
                     LLALPLREAREHFERTYLQQQLLLCQGKVGLLAKRVGMERTHLYRKLRSLGVEFRNIA
                     DD"
     gene            complement(2485..4707)
                     /locus_tag="PKN91_01330"
     CDS             complement(2485..4707)
                     /locus_tag="PKN91_01330"
                     /inference="COORDINATES: protein motif:HMM:NF039586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS26740.1"
                     /translation="MVTAHLKRFGSVALITLWVAIGAASLILLAQTAQNSAQFGRLQP
                     WILLVNIIGVLALTMLLVRRITRLVRDYRNHVPGSRLTVRSVATFGALVVAPLLIVYV
                     FSLDFLNRGIDSWFRVEIKQGLTEALDLSRAAVDQRVRAHAGRTEQFASSLAYVADAD
                     IAAAIDEERRARGALELALFGSGSQLIAASSAALLSGPPERPTSEVVRQVGEGRSYVA
                     LEPRADGEYYIRVAAPVGTSGIRRDPRYVMALYEVPPRLAALSEAVQRSYSQYGELAA
                     MRDPLKYSFRLTLTLVLLIAMLAAINGAIVSSQRFVRPVQDLIAGTRAVGKGDFGARL
                     PLPSRDELGYLVQSFNDMTRRLRRAREEANRSQAEVEHERSRLAVILARLSAGVMSVD
                     RQLNVRTANESASAILGVDLAAANGRNLEELAAANPRVEDFLRELRARLGDGREEWRE
                     QIELPGDQGRRVLMCACTPLPGGEDEPGYVFVFDDITALLAAQREAAWGEVARRLAHE
                     IKNPLTPIQLSAERMRRRFLAQMNAADAAVLERATHTIVQQVEAMKQMVNAFSEYART
                     PEMTVTRFALNHLVTEVADLYRAQNAGVALRLELGEEVESVEADRGRVRQVLNNLLAN
                     AFEAVEGRENARIGVATSLGGEAGEPVVTIEVSDNGPGFRPEIIDRAFDPYVTSKTKG
                     TGLGLAIVRRIAEEHGGHVEVENLAEGGACVRVRLPLTEAGRTLAGRERRNDLRRERA
                     "
     gene            complement(4689..5267)
                     /locus_tag="PKN91_01335"
     CDS             complement(4689..5267)
                     /locus_tag="PKN91_01335"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HNS26741.1"
                     /translation="MTRAHIIPAALLACFGVATSAFGNALDGVLAVRSAYVNVDSGVF
                     KLHARVEYPETEDVRTALGDGVTLMFDLEANVARERRYWWDAGVVANSLRRELSYHAV
                     SNRYVVRDTGGAIGGRQDSYASLHEALTAIGTVDDWPLLVEPQISPDARYRISVRASV
                     RRGRLPDTLRALMFWSDGWHRTSEWYSWSLPI"
     gene            complement(5264..6523)
                     /gene="rsmB"
                     /locus_tag="PKN91_01340"
     CDS             complement(5264..6523)
                     /gene="rsmB"
                     /locus_tag="PKN91_01340"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF008149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNS26742.1"
                     /translation="MNGTSVLTDAARAVARVAHERRSADEVLGSNAAVRAIALGTLRW
                     YLRLAPAIERLLTRPSASMAPAVHALLVTAAHQIVYSRNAPEGVVDAAVDAARALGAP
                     RASGFVNALLRRFVREQAAIFAALDSDPAVRYAHPRWFVAALGREQPARRDDILAGGN
                     ERAPMTLRVDLSRSSVPEAQAACAARGIAAQPLAGYPSALVLDAPVPVDELPGFDEGL
                     LSVQDAGAQLAATLLAPRAGMRVLDACAAPGGKSGHLLEVAGADIDLLAVDVDDERLA
                     RVAANLARLGRRARLACLDLRDPAALAGEAPFDRILLDAPCSATGVIRRHPDIKLLRR
                     AADIPAFAKLQGAILRNCHARLAPGGLLLYATCSVLAAENDAVVDRFVGETPDARRAA
                     DPVQLWPGLRAPTDGFHYALLTKADPA"
     gene            complement(6520..7452)
                     /gene="fmt"
                     /locus_tag="PKN91_01345"
     CDS             complement(6520..7452)
                     /gene="fmt"
                     /locus_tag="PKN91_01345"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNS26743.1"
                     /translation="MRIAFAGTPGFALPPLRALGARHSIVGVLTQPDRPAGRGRALTA
                     SPVKLEAEARGWPLAQPVTLKSEEDRAVLRAWQPDVLVVVAYGLILPQAALDIPRLGC
                     INVHASLLPRWRGAAPIQRAILAGDADSGVSIMRMEAGLDTGPVYAMRRVPIGPRMTG
                     GELHDALAAEGAVALIEVLDAIEAGTARAVPQSAAGVTYAAKIDKAEARIDWARSAIE
                     IDRQVRAFNPWPIAETRYAGEQLRILRAQPDAAAGAGRAAAPGTVLGAGAQGIRVACG
                     SGVLAIESVQRPGRKPVRAADFAHATPLEGVVLG"
     gene            complement(7471..8016)
                     /gene="def"
                     /locus_tag="PKN91_01350"
     CDS             complement(7471..8016)
                     /gene="def"
                     /locus_tag="PKN91_01350"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009682343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNS26744.1"
                     /translation="MALLPILEYPDPRLRTRAEPVTVFDEAFGRLVDDMFETMYAAPG
                     IGLAATQVDVHRQLIVIDASEEKNERHVFVNPVILERSGTAVTEEGCLSVPGIFDEVQ
                     RASHVRVRARDRHGIEFEQELEGLLAVCLQHEMDHLDGKLFVDYLSDLKRERIRKKLD
                     KERKARGTSTAAPVPARVPAL"
     gene            8202..9428
                     /locus_tag="PKN91_01355"
     CDS             8202..9428
                     /locus_tag="PKN91_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNS26745.1"
                     /translation="MFSNQTVSPAAVWSRVAVFACAALITACSTSEPVVYETAPQQEY
                     VSDAPVPGGAGQDLTATEAATILSEGAAPSGSTITSDVIRPDAPSRYTVKRGDTLWDI
                     SSLFLRDPWLWPEIWIVNPQVENPHLIYPGDVLALAYGADGRPQISLEQGGAARLNPR
                     LRSTPGDGPIATIPYSAIAAFLSRPSLLSAEQIEDAPHVLAFRNEHMVGGTGQQIYVR
                     NLDAPIDARFAVVHPMQKLRDPDNGDVLGYQGVYTGTARVTRTGDPLTATLLDATRET
                     LEGDILIANDSDVPLTFEVRVPNIDVNGSIISVLDDTTLIGTYQVVALNRGTRHGVGP
                     GHVLAIDRAGPTVRDRGRRAEGRLERSSAFSDKVTLPNERAGTALVFKSYDRMSYAIV
                     VGASEAIRVADIVRRP"
     gene            complement(9439..10080)
                     /gene="maiA"
                     /locus_tag="PKN91_01360"
     CDS             complement(9439..10080)
                     /gene="maiA"
                     /locus_tag="PKN91_01360"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_026437509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HNS26746.1"
                     /translation="MRLHGYWRSSAAYRVRIALNLKGVACEQAAHHLVRGEQRGPDYL
                     ALNPQGLVPALETGGDVLTQSLAIIEYLDETIPEPALLPRDARARAQVRAMALSVACD
                     IHPLNNLRVVDYLRRELAQPEGAVERWMRHWIETGFAALEAAVRRHSRDGRHCHGDAV
                     TMADVFLAPQMYNARRVGCDLAGVPTLVAIDAALMALPAFAAAAPERQPDAPA"
     gene            10168..11286
                     /gene="dprA"
                     /locus_tag="PKN91_01365"
     CDS             10168..11286
                     /gene="dprA"
                     /locus_tag="PKN91_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNS26747.1"
                     /translation="MLPDSSTLTLARCPGLTVEQLSAALAQAGNEPARVLRLGEARLR
                     ACGWRDAALAWALAPDAAEVDLDAAWLAASGARLVSCFAADYPPLLREVRHAPAFLYV
                     LGDVGLLSSVQLAIVGSRNPTAGGRADSFEFARYLAGAGLTITSGLALGIDAAAHRGA
                     LKAGGRTVAVCGAGLDLTYPREHAALAAEIAATGALVSEFAPRVPPLPTNFPQRNRVI
                     SGLSLGVLVVEAARRSGSLTTARHAGEQGRETFAIPGSIHNPLSRGCHQLIRSGAKLV
                     EAASDILEELKIPFSRQTLMAAEAPAPGADAAAPRLDKDYEILLDALGFDPTGVDTIV
                     ERTGLAADSVASMLLILELEGRIAPYPGGRYGRLQQKT"
     gene            11283..11759
                     /locus_tag="PKN91_01370"
     CDS             11283..11759
                     /locus_tag="PKN91_01370"
                     /inference="COORDINATES: protein motif:HMM:NF016264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HNS26748.1"
                     /translation="MITGSVLDILIYVFDHYMLEDTPDVPEREALAQDLEQAGFSAPN
                     VERALDWLADLAGERHRPDFAMGKRAIRVYADGELARMSTESRGFLLTLERTGILTAA
                     QREIVIDRLLALDTDELSIDQLKWVVLMVLSSQPGQEQAYARMEDLVFDADGSVPH"
     gene            11850..14357
                     /locus_tag="PKN91_01375"
     CDS             11850..14357
                     /locus_tag="PKN91_01375"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HNS26749.1"
                     /translation="MASNLVIVESPAKARTIKKYLGKDFEVLASYGHVRDLVPKEGAV
                     DPDNKFTMQYRVLEKNEKHVDAIARALKKADALYLATDPDREGEAISWHLSELLRERG
                     ALDGKDVHRVAFYEVTKNAIRQAIEEPRGLSMDLVNAQQARRALDYLVGFNLSPLLWK
                     KVRRGLSAGRVQSPALRMICEREEEIQRFQAQEYWTIDAEGEHSGQQFPLKLVEYDGR
                     KVEQFSFTSGEVAHAVERALRAAAGGQAEGSAGTLTVSRIDRKQRRRNPAPPFTTSTL
                     QQEAARKLGFSAQRTMRLAQQLYEGVDYGEGSTGLITYMRTDSVSLAAEAVTDIRSVI
                     AQLYGKDSLAEDVRVYKTKSRNAQEAHEAVRPTSAAITPQLLEGRIDPDQWKLYSLIW
                     KRAVACQMAHALFDTVAIDMQAGAHTLRANGSTLVKPGYIAVYQEGRDDAVEDDSDHV
                     LPPMKEGDTVKLVAIHPEQHFTEPPPRYSEASLVKALEEHGIGRPSTYASIISTLKDR
                     EYVEMDNRRFIPTDIGKIVNGFLSKHFHRYVEYGFTAAMEDELDAVSRGEEDWTQPLK
                     KFWKPFIDLVEHTEKHVSRDEVAQARELGKDPASGKPVTVRMGRFGPFVQIGTKDDEE
                     KPRFAGLRPGQKMDKITYEEAMELFKLPRNLGTTAEGEPVQANIGRFGPYVKYGAKYA
                     SLKEDDPYTITLERALEVIAAKKLADANRIIQDFGVDDIQVLNGRYGPYITDKKKNAK
                     IPKDRDPKTLTLEECRAMLAAAPERGGRFGRRGAAARGKKAAAAAPAAPAADAPAEAP
                     AAKKKAAPKKKAAASKKAAAKKKAPAKKKAAAEAKPA"
     gene            14370..15233
                     /gene="rlmJ"
                     /locus_tag="PKN91_01380"
     CDS             14370..15233
                     /gene="rlmJ"
                     /locus_tag="PKN91_01380"
                     /inference="COORDINATES: protein motif:HMM:NF016278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HNS26750.1"
                     /translation="MKYRHAYHAGNFADVHKHVALVALLRALQRKDKGFLYVDTHAGR
                     GRYALGAGFARESGEAQSGAVAVLAALRDSAGAPDELLDYAAVIEAWRRAADARHDYP
                     GSPLIAAQLLRPQDHGVAIESQPAEFEALRKALGRASRVVAEHGDGYQRLRALLPPPS
                     RRGLVLIDPPYEDTRGDFRAAILAVQHALARFEGCVIALWYPVKDARDTDRWLQELAR
                     GLPRPALAAELWVHPPDSRAGLNGSGLAVVNPPWLFADRCDIWQGALRPLLDPAGAGG
                     TRVVSLVGESR"
     gene            15243..16088
                     /gene="aroE"
                     /locus_tag="PKN91_01385"
     CDS             15243..16088
                     /gene="aroE"
                     /locus_tag="PKN91_01385"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001310.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNS26751.1"
                     /translation="MGADPQAEVDSYGVIGHPVAQSRSPFIHGLFAKQTGQRMVYRLF
                     DVTPDDFLNHVTGFFAGGGRGLNVTVPHKPAAANFASTLTPRAEQARAVNTLALQADG
                     SVLGDNTDGVGLVRDLTINLGLVVTGLRLLILGAGGATRGIVAPLLDLTPAEMLIANR
                     NGDRARVIAASFADLGPVKGCGFDEIDARPWDLVINATAASLAGARPEVSPTIIGPHT
                     VCYDLAYGREPTPFLNWALAEGASAAHLGWGMLVEQAAESFYLWRGVRPDTRPVLEVL
                     VPGTR"
     gene            complement(16144..17493)
                     /gene="gorA"
                     /locus_tag="PKN91_01390"
     CDS             complement(16144..17493)
                     /gene="gorA"
                     /locus_tag="PKN91_01390"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF004776.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HNS26752.1"
                     /translation="MLEKYDLAVIGGGSGGLACAQRAAEYGARAVVIESGRLGGTCVN
                     VGCVPKKVMWNAAELAAGLLDAADYGFALDVRGLDFAALKARRDAYIVRLNDIYARNL
                     EKRGVALVRGHAQLTGPHEIRVGEQVLAADRIVIATGGRPSRIRLPGEELGIDSDGFF
                     ELEALPPRALIAGSGYIAVELAGILAALGSQVTLVIRRERVLRSFDVMLGEGAMRALR
                     DSGVSIVTGRTPTSLARKGEALELTLDDGSVLPPVDCLLWAVGREPMVENLGLSRAGV
                     ALGADGFIAVDKYQQTNLPHVFAIGDVTGRLQLTPVAIAAGRRLSDRVFGGMAGRHLD
                     YDNVPTVIFTHPPIGTVGLTEAEARLRHGDGSVKTYTSGFVPLYHALTSRKPRAEMKL
                     VVAGPEERIVGLHVIGEGADEMLQGFAVAVKMGATKRDFDDTVAIHPTSAEEFVTMR"
     gene            17525..19543
                     /locus_tag="PKN91_01395"
     CDS             17525..19543
                     /locus_tag="PKN91_01395"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNS26753.1"
                     /translation="MTNPLLTDDLLPRFDAIRPEHVAPAIRGLLAENRARLAAIAAET
                     TPTFASVVEPMEEMSHRLQRAWSPVSHLNAVLNSAPLREQYNLCLPLLTDYQTDLAQS
                     EPLYKAYVAVAEREAATLDPVQRRVVDHMLRDFRLAGVALEPARKERFKAVMLELTTL
                     QSKFEENVLDATNAWSHHVTDPAELAGLNEGVVEQARRRAHEKRTEGWLLSLDQPTYV
                     AVITDAESAALRRAYYEAWNTRASDQGPLAGRWNNTQVMDDILRLRHEAATVLGFANF
                     ADYALATRMAKSVPEVIDFLRELARAARPAAEREFAELEKFAGAKLDAWDLAYWSERL
                     QRSRFSVSQEELRPYFPLPRVLAGLFGVAEQLFGVHIAERGGVTTWHPDARYFEITDA
                     RGAPVGSFYLDPYARPHKRSGAWMDDCVGRKVSASGVTNPVAYLVCNFLPPSGEQPAL
                     LTHDDVVTLFHEFGHGLHHMLTRVGYPSVAGINGVAWDAVELPSQFFENYAWNEQVLG
                     AMAAHYATGAPLPADRQRQLIATRSFHAGLATMRQVEFSLFDFRVHAEYDPALGGRIA
                     PILAEVRQEVGVVPVPAWNRFPHSFGHVFAGGYAAGYYSYKWAEVLAADAFAAFEEAG
                     VFDRATAARFLDSILSRGGSRDPLEAFIEFRGRKPDIAPLLRQHGIAA"
     gene            19549..20319
                     /gene="xth"
                     /locus_tag="PKN91_01400"
     CDS             19549..20319
                     /gene="xth"
                     /locus_tag="PKN91_01400"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNS26754.1"
                     /translation="MRTIATWNVNSLRVRLPQLTEWLASNPVHTLALQETKLEDKAFP
                     RAEIEALGYQVAFSGQRTYNGVAILARAPLGDLVTDIPGFEDPQRRVLAATVDGVRVV
                     NLYVPNGQAVGSEKYAYKLRWLAAVRDWLAAELAQHRQLIVLGDFNIAPEDRDVHDPA
                     AWEGSVHVSEAERAALRELLALGLTDSFRLFEQPEGSYSWWDYRQGAFRRNAGLRIDL
                     LLTSKALTERCKAASIDREPRRAERPSDHAPASASFDI"
     gene            20415..21413
                     /locus_tag="PKN91_01405"
     CDS             20415..21413
                     /locus_tag="PKN91_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26755.1"
                     /translation="MHNNRRSDFDVTNTVQVSSPGDVCEAVKKLFTATWPGAPFEPVA
                     RGFDRLEEVFSGRDPEYHALDTVYHDTQHTLDITLAMARLCAGYEREAPPGRALGAER
                     ATVGLLIALFHDVGYLRERSDTVTQNGAELTATHVARGARFLEKYLPAAGFEDWVPIA
                     TQIIHFTGYEVPLDAIQVFDERDRKLGHLLGTADMIAQMADRCYLEKCRDRLYPEFVL
                     GGYALPLSNQGSRQVKYASGLDLLRQTPQFITETRMKRLDGAFASAYRHLDALFDGRN
                     PYMEAIERNLVFLNQILRSESWRMLRRNPPVFAAGEDPMGTMRGLAMGYIRRAWTQ"
     gene            complement(21418..22086)
                     /locus_tag="PKN91_01410"
     CDS             complement(21418..22086)
                     /locus_tag="PKN91_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNS26756.1"
                     /translation="MNALELTGRARTHVIEVPQLRCIVHHDVVAPLLALCAAARSEAG
                     IELAPVSSFRDFERQREIWNAKYRGERPLLDRRGEPLDALALAPDARIDAILAWSALP
                     GASRHHWGTDIDVIDRAALPAGYRTQLIPQEYAPGGVFARLDAWLAESMQRFGFFRPY
                     TRAHDGVQPEPWHLSYAPVARPACAALTVEVLREAIEGAGVEGEEVLIGRLAQLRERY
                     VEER"
     gene            complement(22083..22730)
                     /locus_tag="PKN91_01415"
     CDS             complement(22083..22730)
                     /locus_tag="PKN91_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HNS26757.1"
                     /translation="MLVEVFSDTVCPWCYVGKHRFEQALGQRADLAVEVRWLPFELNP
                     DMPVEGADRAQYLAAKFGSSDRFADAQRSLRELGPGLGIDFHFERITRMPNTRRSHAL
                     IAWAGGASPAAQADAQERVLAAYFSEGRDIGSPEVLVAIAGEAGLDAAAARTAIDDPL
                     LHQQIATLEAQAHTWGIHGVPAFVFARRYLVSGAQEPEAFLQVIDRVVAEGATGT"
     gene            22881..25337
                     /locus_tag="PKN91_01420"
     CDS             22881..25337
                     /locus_tag="PKN91_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNS26758.1"
                     /translation="MAVAVAAMLAATVLLAGLLLWHARAESQLVSAIATEEHRNAIGT
                     ELAARAEAIATHVADVAAPAVVAGNRATLAERMEALGDDAAITGIEILDAEGASLYVL
                     RRAAPDSEAVITRSYTQPIRAMLPINSGTTAAQSVGTARVTLTTRDTSPADIGAPVAA
                     AGRSMLNRALFGLGVILVLGLVSAVALAWWAARRIERPISALIRSADRIGQGDLSRPT
                     DLALRDELGELQQALERMRNKLRQTTITKNYLNSVLTGMTDAVFVTSPDGVIRVANQA
                     AQRLLGYGEDELNGQSIVSLLDGPDRENFNVLQASNETREAVVRTRKGQTIPVSLTGS
                     AIATDDPQFQGCIFVARNITDRKRAERRIRYLARYDALTKVLNRMQFQHLLQQAIARA
                     LRNNQTVALLYLDVDHFKEVNDTFGHAAGDRTLEILTERLTRALPKDSVVGRLAGDEF
                     AVLIDGLSGDVDHRGPIGQLARSILAETDKIFQLGQHELFLTTSIGIAFCPRDAENVI
                     DLIRNADAAMYYSKQNGGNTFAFYAPEMNAASVERLMLKSKLRRALERDELIVNYQPK
                     IDLRTGRIVGAEGLLRWRLPGHGDIPPSQFIPLAEESNLILEIGEWVLNRVCLDYRTL
                     RREVADPGRISLNLSLKQLRQASFIVRCRSVFRRHGVSPSAFELEITESTLMADAKRT
                     VHLLGELHAMGLHLSIDDFGTGYSSLSALQQFPIGTLKIDQSFVQNVSTHPGDATIVR
                     TIIDMGRSLGMDVIAEGVETTAQLEFLRDHHCDYGQGRLFGDTLPLEDLLELLMQQQN
                     GTPPFAALIPPAARSAGLSA"
     gene            25353..27266
                     /locus_tag="PKN91_01425"
     CDS             25353..27266
                     /locus_tag="PKN91_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS26759.1"
                     /translation="MLTLRDVSLRRGPRVLFEQASVGVFRGDKVGIVGRNGSGKSSLL
                     ALIRGELAPDVGDYDAPANLCIAIVEQSVPDSAQSVVDYVREGDTELIRIEHAIAAAH
                     AAGDGHREAQLHADYDAAGGYGARSRAASLATGLGFEADDIERPLHVFSGGLRMRAAL
                     ARALMRRSDILLLDEPTNHLDLDAVLWLEGWLRAYRGTLLIVSHDREFLDGVVSRILH
                     IEGGRLTAYNGNYSSFERQYAAARERNAALAESQRREIAHVESFVERFRAKASKARQV
                     QSRIKWLERLPAIIEAQGEEAFAWEFPAPAKLPRPLVALDRVSAGYGGRAVFTGLGLS
                     VAPGDRIGVLGRNGAGKSTLMKTVAGALAPLAGERVASPDLEVGFFAQLEVEQLDAAS
                     SSIEELARRGGPQVAAWTPQQQRDHLGRFGFRGDRVFEAVRNFSGGERARLTLAIMVA
                     RRPNLLLLDEPTNHLDFEMRNALLLALQDYAGAVLIVSHDRGLLRGACDRFMVVAGGS
                     VRPFDGDLEDYAAWLAKGAPTGDGKASGRAGDSRDGAERTAQPQRRDRDTRRREAAAR
                     NRLSPLRIELRSIEDELAQLATQRAALERELADDPTKYALATDYERLAARTTSLEERW
                     LELSEEIDAAQRA"
     gene            27247..28758
                     /locus_tag="PKN91_01430"
     CDS             27247..28758
                     /locus_tag="PKN91_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNS26760.1"
                     /translation="MPPSARKRWLRWTGGALLAVWLGVMAWNTWKPLPEGVTVESGWS
                     PLQEGDVQFLRDITAADAYGHPIVDQQIFEAVFELIAEARHFILIDMFLFNAHRGADV
                     AAPVHRTLAAELTNALLERRRADPGLRIVFITDPINDVYGGAPSPELQTLRDAGVEVV
                     VTDLDRLRDSNPLYSAFWRIALRWWARGQPGTWPDPFDAQGPGVSMGAWARLVNFKAN
                     HRKVLIVGREDGTLAGIVTSANAHDASSSHSNVALRISGPVLEPLIASELAIARYSGW
                     EGDPLPVPGTAPTAATETASATPSSPSLPGRARMLTESAIADAVVARLQTCVAGDAID
                     VAMFYLSDRRVLGALRDAATRGAAVRVLLDPNKDAFGRQKNGIPNRPVATELVAATDG
                     AIRLRWYRTHGEQFHVKLLAIRGRDDFWLTLGSANFTRRNVRDYNLEANIAIELPRDS
                     ALELAVLEWFDGLWMNRAPAGVEYTAEYGTYADPRQIRYWGYRVMEATGLSTF"
     gene            complement(28786..29193)
                     /locus_tag="PKN91_01435"
     CDS             complement(28786..29193)
                     /locus_tag="PKN91_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNS26761.1"
                     /translation="MEPRIIKTDKQYRRFLSEVEALAAGDPDPESKKGIRLELLAKLV
                     EDYEKTRFPFEKPDPVDAILFRMEQQGLRQKDLAVILGGKNRASEVLARKRPLTLPMI
                     RSLYERLEVPAGLLIKEPAPAAYSRPRRSRSRT"
     gene            complement(29211..29432)
                     /locus_tag="PKN91_01440"
     CDS             complement(29211..29432)
                     /locus_tag="PKN91_01440"
                     /inference="COORDINATES: protein motif:HMM:NF021431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HNS26762.1"
                     /translation="MAWLAETESGSWDTPQTIKDRYSSASFVGSNTVIFNVKGNAYRL
                     EVQVAYRAGVVVVNWIGTHAAYDRRNAKP"
     gene            complement(29678..30589)
                     /locus_tag="PKN91_01445"
     CDS             complement(29678..30589)
                     /locus_tag="PKN91_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNS26763.1"
                     /translation="MREVAPGDIVFSFYDTRISALGIVSGYCRESPKPEEFGNAGTNW
                     SQIGWRVGVHWQRLSSSICPRDHIARLRPYLAERYAPLRSDGNGLQAVYLTEINQGFA
                     STLFGLIGSEAYQVADTGRQVSEVERDSPAPERDIEEWERRLEVEIAASAEIPETERT
                     ALVQARRGQGLFRENVRSIERACRVTRVERLEHLIASHIQPWRDSSNEQRLDGENGLL
                     LTPTIDHLFDKGFISFENSGQLIVSPVADPVSLKRMGLNPQANLNVGAFSQGQQQYLE
                     FHRENVLRMAGGGAGAVLVDIGGSDRA"
     gene            complement(30624..31442)
                     /locus_tag="PKN91_01450"
     CDS             complement(30624..31442)
                     /locus_tag="PKN91_01450"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNS26764.1"
                     /translation="MADDWRKASIGALCDAGDAELQTGPFGTQLHAYDYVDDGVPVVP
                     TEAIRARQIDHSVLPKITQTKAKELERHRLRPGDILFARRGVQAAGHIGCVRKAEDGF
                     ICGTGAIRLRVARDSDAIDPAYLSHVLADPSSVQWFKFHAIGATMPNLNEGIIRSFPL
                     SVPPLSEQRAIAHILGTLDDKIELNRRMGETLEAMARALFKSWFVDYHPVRAKAEGRD
                     TGLPKPLADLFPARLVDSDVGEIPDGWEVARLTSAVEVNPPRSLRKGVSLVAQA"
     gene            complement(31432..32927)
                     /locus_tag="PKN91_01455"
                     /pseudo
     CDS             complement(31432..32927)
                     /locus_tag="PKN91_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
     gene            33185..>34857
                     /locus_tag="PKN91_01460"
     CDS             33185..>34857
                     /locus_tag="PKN91_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HNS26765.1"
                     /translation="MTTFLTSPGGSGTGATPGERRFAERLGALLEDDYLCWYDVPVGP
                     RHQHPDFLLLHPGRGLLVLEVKDWKLDNIHELTTQSVTLNLGSGLKTKANPLEQARHY
                     AHAVVKVLERDPRLLVPPGEKYEGHLRFPYGYGVVLTNITRLQFERSGMAETIPPHRV
                     ICRDEMTESADAETFQKRLWDMFSVRFQSQLTLPEVERIRWLLFPEIRIHQQGLPIEA
                     QADDARAPDSLQVMDLAQEAIARGLGDGHRVIHGVAGSGKTLILAYRCQYLAATLARP
                     ILVLVYNKALAAWLQHQMALRGLSDRVTVRNFHGWCHDMLRLYHVPKPADGKDFYPAL
                     VNAVIRAVDRGQIPRAQYGALLIDEGHDFEPDWLRLAVQMLDPESNQLLLLYDDAQSI
                     YGAKRKGFTFRSVGVSAVGRTKILKRNYRNTNEILACAAEFARELLQEVDSDEDGVPL
                     VSPVSGGRSGPPPKFYDARTLEQEAACIARQLQRRHQSGTPWREMGVFYSAPFVAETV
                     ANSLTDAGIPYDWLKDSSSKKWDPDKDSVKLMTIHSSKGLQYQVGIIAGA"
BASE COUNT         5665 a        12410 c        11552 g         5230 t
ORIGIN      
        1 gatcgaggcg caggctcgcg aacacgggct cagcaccgag cgcgtgatca gcgagatcat
       61 gctgaagccg atgccgaaga aagccttcgt gacggtggag gagatcgctg cgctcgcggc
      121 ctacctgatc agcgacgcgg cgcgcaatgt gacggcgcag gcgatcgcga tcgacggggg
      181 ctggaccgcg cagtgagggc gtgggcgcgg cgccgccgcc cctaccccct cgcgagcgcg
      241 aggctccacg ccagtaccac caggccgatc gcgagcgcgc caccgaccca ctgcggcaca
      301 cgcgcgagcg ccgcctcgac gcggctgcgc gcgatcgcca gccccgccgc cgcgccgcag
      361 ctgaagccga ggatgtgcgc catcacattg gtgtcttcgc cggagctgcc gagccagccg
      421 agcagcagga cgccgccgac gagcggcgcc cagcgcacgg cccagcgcgc gcgcaacgga
      481 aaatgctcgc gccaggagtg cgcggcgagc aacccgagcg ccgcaaacac cgcggtcgac
      541 gctccgaccg agcgatacgg caccgggccg agcaggccct ggatcagatt ggcggccatc
      601 gcggcaagca cgatcagtgc ccaggcggcg cccgggccga gccgccgtcc ggcgagatag
      661 ctgaaccagg cgccaccacc gatgttcgcg acgagatgcg gcgcatcgac gtggagcgtt
      721 agcgcggtga tcgcacgcca ccactcaccg gcgcggatca gccccgcgtc ggcgaccccg
      781 cgcgcgtagg catcgagcgg accgaggccg gcgccgaccg cgagggtgac ggcgagcagc
      841 accaccacgt acagcaggct gccgaaccag gcgaacggat gcgcgatccg gcgcggcggc
      901 gaggccgcgc gcacggcgag gcgctcggcg gcatagcgcg cgaggtgctc gcgcgcggcc
      961 cgggcctgcg ccggatcgac cagcagcgcg tgcccggccg gcgcgcgcgc gatctggaag
     1021 ggaatgccga ccgcggtcag cacgaacgcg cgctcgttgc aggccgcgaa gtcgtccgca
     1081 acgaacaccg ccacgggcgc ctcgccatgc ggcgcgccgt gctcgtccat cagtcgtcgg
     1141 cgatgttgcg gaactcgacg ccgagtgaac gcagcttgcg gtaaaggtgc gtacgctcca
     1201 tgccgacgcg cttggcgagc aggccgacct tgccctggca cagcagcagc tgctgctgca
     1261 ggtaggtgcg ctcgaaatgc tcgcgcgcct cgcgcagcgg cagcgccagc aggtcctgct
     1321 tcacgagcgg ctcttcctgc ggcgtgcgca ccgcgagttc gcgctcgact tcctcgagct
     1381 cgatctcctc gggcccgccg cgcagcagct ggcgatgcac gagattgtgc atttcgcgga
     1441 tgttgccggg ccacgggtag ttgcgcaggc ggttctgcgc cgccacgctg aatctgcgaa
     1501 accgcagacc ctcggcatcc accaggcgat cgacgtagta gcgcagcagc tccggcacgt
     1561 cctcggcgta ctcgcgcagc ggcggcacca ggaagatcag cgtattcagt tgcgacagca
     1621 ggtcgcggcg gaagctgccg tcggccgcgc gctgctcgat gccgggctgt gccgaagcga
     1681 atacgcgcgc attgaaggcc acgggctcgg tgccaccgac ccgcgtgaag tgcatggtct
     1741 cgagcacgcc gagcaggatg cgctgcgcgc ttgcgggcag gtcgtcgatt tcgctgatga
     1801 acagcgtgcc tcccgcggcc tgttccagca cgccgggctt cacgacgccg cccgattcgg
     1861 agccgagcag cagcggctcg ggatcgctcg agctgacggt gctcgccacg agcgtgacca
     1921 ccggctgcga cgcatccgga cccgcctcgt gcagatagcg cgcgaacgcc tcgcggccgc
     1981 tgccggactc gccgatgaac agcacgcgct ggctgttggc gagctgcgcg agctcggtgc
     2041 ggagctggcg catcaggctg ctcttgccga ccgggaccat cagcggaggc acggtcaggc
     2101 gcccgccgcg gcggcggcgg cccgcgtcga gcgcgcgctc gacggtgcgc agcagcttcg
     2161 cgagcgacaa cggcttctcg acgaaatcga atgcgccgag gcgcgtcgcc tcgaccgccg
     2221 tctcgacggt accgtgaccg gacatcatca ccaccgggca gctctcgccg ccctgcgccg
     2281 accattcgcg caacagcgtg atgccatcga cgtcgggcat ccagatgtcg agcagcacca
     2341 ggtcgggtgc gcggttcgcg cgcgccgcgc gcgcctccgc ggcattggcc gccgcctcga
     2401 cctcgtagcc ctcctcggtg aggatctcgg tgagcaatcc gcggatgtcc gactcgtcgt
     2461 cgaccaccag aatgtgagct gcactcatgc acgttccctt cgcaaatcgt tccggcgctc
     2521 gcggccggcc agcgtgcgcc cggcttcggt gagcggcagc ctcacgcgca cgcacgcccc
     2581 gccctccgcc aggttctcca cttcgacatg cccgccatgc tcttcggcaa tgcggcgcac
     2641 gatggcgagg cccagccccg tccccttggt cttgctcgtc acgtacggat cgaacgcccg
     2701 gtcgatgatc tcgggccgga agccgggccc gttgtcactg acctcgatcg tcaccaccgg
     2761 ctcgccagct tcgccgccga gcgaggtggc taccccgatc cgcgcattct cccggccctc
     2821 gaccgcctcg aaagcgttcg ccagcagatt gttcaggacc tggcgcacgc gcccgcgatc
     2881 cgcctcgacc gactcgacct cctcgccaag ctcgaggcgc agcgcgacgc cggcattctg
     2941 cgcacgatac aggtccgcca cctccgtgac gaggtggttg agcgcgaacc gtgtgacggt
     3001 catttccggc gtgcgcgcgt actcgctgaa cgcattcacc atctgcttca tcgcctcgac
     3061 ctgctgcacg atggtatgcg tcgcccgctc gagcacggcg gcgtccgcgg cgttcatctg
     3121 tgcgaggaaa cgccgtcgca tacgctcggc ggacagctgg atcggcgtga gcgggttctt
     3181 gatctcgtgc gcgaggcggc gcgcgacctc gccccaggcg gcttcgcgct gcgcggcgag
     3241 cagcgcggtg atgtcgtcga acacgaatac gtagccgggc tcgtcctcgc cgcccggcag
     3301 cggcgtgcag gcgcacatca gcacgcggcg cccctggtcc ccggggagtt cgatctgctc
     3361 gcgccactcc tcgcgcccgt ccccgaggcg cgcgcgcagt tcgcgcagga aatcctcgac
     3421 gcgcggattc gcggcggcga gctcctcgag gttgcgtccg ttcgcggccg cgaggtcgac
     3481 gccgaggatc gcgctcgccg attcgttggc ggtgcgcacg ttgagctggc gatcgaccga
     3541 catgacgcct gccgacaggc gcgcgaggat caccgcgagg cggctgcgct cgtgctcgac
     3601 ctcggcctgg ctgcgattcg cctcctcgcg cgcgcgccgc agccgtcgcg tcatgtcgtt
     3661 gaacgactgc acgaggtagc cgagctcgtc gcgcgagggc agcggcaggc gcgcgccgaa
     3721 gtcgcccttg ccgaccgcgc gcgtgccggc gatcagatcc tggaccggcc ggacgaaacg
     3781 ctgcgaggag acgatcgcgc cattgatcgc cgcgagcatc gcgatcagca gcaccagggt
     3841 cagggtcagg cgaaagctgt atttcagcgg atcgcgcatc gcagcgagtt cgccgtactg
     3901 cgaataggag cgctgcacgg cctccgacag cgcggcgagc cgcggcggca cctcgtacag
     3961 cgccatcacg taacgcggat cgcgccggat gccggacgtg ccgaccggcg cggcgacgcg
     4021 aatgtagtac tcgccgtccg cgcgcggctc gagcgcgacg taggagcgcc cctcgccgac
     4081 ctggcgcacg acctcactgg tgggccgctc cggcggaccc gacagcagcg cggcgctgct
     4141 cgccgcgatc agctgcgagc ccgaaccgaa cagcgccagc tcgagcgcac cgcgcgcacg
     4201 gcgctcctcg tcgatcgccg cggcgatgtc ggcgtcggcg acataggcga ggctgctcgc
     4261 gaactgctcg gtcctgccgg catgcgcgcg cacgcgctgg tcgaccgccg cgcgcgacag
     4321 gtcgagcgcc tcggtgaggc cctgcttgat ctcgacgcgg aaccagctgt cgatgccgcg
     4381 gttcaggaag tcgagcgaga agacgtacac gatcagtagc ggcgcgacca ccagcgcgcc
     4441 gaacgtcgcg acgctgcgca ccgtgaggcg cgagcccggc acgtggttgc gatagtcgcg
     4501 cacgaggcgc gtgatgcgcc ggaccagcag catcgtgagc gcgagcacgc cgatgatgtt
     4561 gaccagcagg atccagggct gcagacgccc gaactgcgcc gagttctgcg cagtctgcgc
     4621 cagcaggatc agcgaggccg cgccgatcgc gacccagagc gtgatgaggg cgaccgatcc
     4681 gaagcgtttc agatgggcag tgaccacgag taccactcgc tcgtgcgatg ccagccgtcg
     4741 gaccagaaca tcagcgcgcg gagcgtgtcg ggcaggcggc cgcgccgcac gctcgcgcgc
     4801 acgctgatgc gatagcgcgc gtcgggcgag atctgcggct cgacgagcag cggccagtcg
     4861 tcgaccgtgc cgatggcggt gagcgcctcg tgcaggctcg cgtagctgtc ctgccggccg
     4921 ccgatggcgc cgccggtgtc gcgcaccaca tagcggttgc tgaccgcgtg gtacgacagc
     4981 tcacggcgca gcgaattggc cacgacgccg gcgtcccacc agtagcggcg ctcgcgcgcg
     5041 acgttcgcct cgagatcgaa catcagcgtg accccgtcgc cgagcgcggt gcggacgtcc
     5101 tcggtctcgg gatattccac gcgcgcgtgc agcttgaaca cgccgctgtc gacgttgaca
     5161 taggcggagc gcaccgcgag cacgccgtcg agcgcgttgc caaaagccga cgtcgcgacg
     5221 ccgaagcacg caagcagggc tgcggggatg atgtgtgcgc gcgtcatgcc gggtccgcct
     5281 tggtcagcag agcataatgg aagccgtcgg tgggtgcccg cagtccgggc catagctgca
     5341 cggggtccgc cgcgcggcgc gcgtcggggg tctccccgac gaagcgatcc accaccgcgt
     5401 cgttctcggc ggcgagcacc gagcaggtgg cgtacagcag cagcccgccg ggggcgaggc
     5461 gcgcatggca gttgcgcagg atcgccccct gcagcttcgc gaaggccggg atgtccgcgg
     5521 cgcggcgcag caacttgatg tccgggtggc gccggatcac cccggtcgcc gagcacggcg
     5581 catcgagcag gatccggtcg aacggcgcct cgcccgcgag cgccgccggg tcgcgcaggt
     5641 cgaggcaggc gagtcgcgcc cggcgcccca gccgcgcgag gttcgccgcg acccgcgcga
     5701 ggcgctcgtc atcgacatcg accgcgagca ggtcgatgtc tgcgccggct acctcgagca
     5761 ggtgtccgct cttgccgccg ggcgccgcgc aggcatcgag cacgcgcatg cccgcgcgcg
     5821 gcgcgagcag cgtcgcggcg agttgcgcgc cggcgtcttg caccgacagg aggccctcgt
     5881 caaagccggg cagctcatcg accggcaccg gcgcgtcgag caccagcgcg ctcgggtagc
     5941 cggcgagcgg ctgcgccgcg atgccgcgcg ccgcgcaagc cgcctgcgcc tcgggcaccg
     6001 aactgcgcga cagatcgacc cgcagcgtca tcggcgcgcg ctcgttgccg ccagccagaa
     6061 tgtcgtcgcg ccgcgcgggc tgctcgcgcc cgagcgcggc gacgaaccag cgcggatgcg
     6121 cgtagcgcac ggccgggtcg gaatcgagcg cggcgaagat cgccgcctgc tcgcgcacga
     6181 agcggcgcag cagcgcattg acgaaaccgc tcgcgcgggg cgcgccgagc gcgcgcgccg
     6241 catcgacggc cgcgtcgacg acgccttccg gcgcgttgcg cgaatagacg atctggtgcg
     6301 ccgccgtgac cagcagcgcg tgcaccgccg gcgccatcga ggcggacggg cgcgtgagca
     6361 ggcgctcgat cgcgggcgcg aggcgcaggt accagcgcag cgtgccgagc gcgatcgccc
     6421 gcaccgcagc gttgctgccc agcacctcgt ccgcggagcg ccgctcgtgc gccacgcgcg
     6481 ccacggcgcg cgcggcgtcc gtgagcacgc tcgtgccgtt catccgagca ccacgccctc
     6541 gagcggcgtc gcatgtgcga aatcggccgc ccgcaccggc ttgcgtcccg ggcgctgcac
     6601 cgattcgatg gcgagcacgc cgctcccgca ggcgacccgg atgccctgcg cgcccgcgcc
     6661 cagcacggtg cctggcgcgg cagcgcggcc ggcacctgcc gccgcatccg gctgcgcgcg
     6721 caggatgcgc agctgctcgc cggcgtagcg cgtctcggcg atgggccacg gattgaaggc
     6781 acgcacctgc ctgtcgatct cgatggcgct gcgcgcccag tcgatgcgcg cctcggcctt
     6841 gtcgatcttc gccgcgtagg tcacgcctgc cgcggactgg ggcacggccc gtgccgtgcc
     6901 ggcctcgatg gcatcgagca cttcgatcag cgccaccgcg ccctcggccg cgagcgcatc
     6961 gtgcaactcc ccgcccgtca tgcgcggtcc gatcggcacg cggcgcatcg cgtacaccgg
     7021 accggtgtcg agacccgcct ccatgcgcat tatcgacacg ccgctgtcgg catcgcccgc
     7081 gaggatcgcg cgctggatcg gcgccgcgcc gcgccagcgc ggcagcagcg aggcatgcac
     7141 gttgatgcag ccgaggcgcg gaatgtcgag cgcggcctgc ggcaggatca ggccgtaggc
     7201 gacgaccacc agcacgtccg gctgccacgc gcgcagcacg gcgcggtcct cttcggactt
     7261 gagcgtcacg ggttgcgcga gcggccagcc gcgcgcctcg gcttcgagct tgacgggact
     7321 tgcggtcagc gcccgtccac gaccggccgg ccggtcaggc tgggtgagga ccccgacgat
     7381 gctgtggcgc gcgccgaggg cgcgcagcgg cggcagcgcg aacccgggcg ttccggcgaa
     7441 cgcgatgcgc acgctcctgc ggtcccgcgc tcagagtgcc ggcacgcgtg ccggcaccgg
     7501 cgcagccgtg gacgtccccc gcgccttgcg ctccttgtcg agcttcttgc ggatccgttc
     7561 gcgcttcagg tccgagaggt aatcgacgaa cagcttgccg tcgaggtgat ccatttcgtg
     7621 ctgcaggcac acggcgagga gcccctcgag ctcctgctcg aactcgatgc cgtgacggtc
     7681 gcgcgcacgt acccgcacgt gcgaggcgcg ctgcacttca tcgaagatgc cgggcaccga
     7741 caggcaaccc tcctcggtca ccgccgtgcc gctgcgctcg aggatcaccg gattcacgaa
     7801 cacgtggcgc tcgttctttt cctcggacgc gtcgatcacg atcagttgcc ggtgcacgtc
     7861 gacctgggtg gcggcgagcc cgatgccggg ggccgcgtac atggtttcga acatgtcgtc
     7921 gaccaggcgg ccgaaggcct catcgaacac ggtgacgggt tcggcgcggg tgcgcagtcg
     7981 cgggtcgggg tattcgagga tcgggagcag ggccatggaa accttcggta atgctgctaa
     8041 tgtattcagc cggctgggtt ttatcggctg gcaaacgggg gtcggggtcg ccggcgcccc
     8101 gtgaaccagt gcacaaagtt gacatcaggc cgtgtgcgcc ggttcccgta gagattataa
     8161 cagcggggtc gcttgcccca cccggtgtag aggagtgagg gatgttttcg aatcaaacag
     8221 ttagccccgc ggcggtctgg tcgcgcgtgg cggtctttgc ctgtgccgcg ctgatcacgg
     8281 cctgctcgac ctccgagccg gtcgtctatg agaccgcgcc acagcaggaa tacgtgtccg
     8341 acgccccggt gccgggcggc gccggccagg acctgaccgc caccgaggcg gccacgatcc
     8401 tgagcgaggg cgcggccccc tccggctcca ccatcacctc ggatgtcatt cggcccgacg
     8461 cccccagccg ttacacggtc aagcgcggcg acacgctgtg ggacatctcg agcctgttcc
     8521 tgcgcgatcc gtggctttgg cccgagatct ggatcgtgaa tccgcaggtc gagaacccgc
     8581 acctcatcta tccgggtgac gtgctcgccc tcgcctacgg ggccgacggc cgcccgcaga
     8641 tcagcctcga acagggcggc gccgcgcgcc tcaatccgcg cctgcgcagc acccccggcg
     8701 acggcccgat cgcgaccatc ccctacagcg ccatcgcggc gttcctgtcg cggccgtcgc
     8761 tgctgtcggc cgagcagatc gaggacgccc cgcacgtgct cgcgttccgc aacgaacaca
     8821 tggtgggcgg cacgggccag cagatctacg tgcgcaacct cgacgcgccg atcgatgcgc
     8881 gcttcgcagt cgtgcatccg atgcagaagc tgcgcgaccc cgacaacggc gacgtgctcg
     8941 gctaccaggg cgtctacacg ggcacggcac gcgtcacccg caccggcgac ccgctcaccg
     9001 ccacgctgct cgacgccacg cgcgaaacgc tcgaaggcga catcctgatc gccaacgact
     9061 ccgacgtgcc gctcaccttc gaagtgcgcg tgccgaacat cgacgtcaac ggttcgatca
     9121 tctcggtgct ggacgacacc acgctgatcg gcacctacca ggtcgtcgcg ctcaaccgcg
     9181 gcacgcgcca cggcgtgggc cccggccacg tgctcgcgat cgaccgtgcg ggacccacgg
     9241 tgcgcgaccg cggccgccgc gcggaaggcc ggctcgaacg cagcagcgcc ttctccgaca
     9301 aggtgaccct gcccaatgag cgcgccggca cggcgctcgt gttcaagagc tacgaccgca
     9361 tgagctacgc gatcgtcgtc ggcgcctccg aggcgattcg cgtcgccgac atcgtccgca
     9421 ggccctgagg cgcgagcctc aggcgggggc gtccggctgg cgctccggcg ctgcggcggc
     9481 aaacgccggc agcgccatga gcgccgcgtc gatcgccacc agcgtcggca ctcccgcgag
     9541 gtcgcagccg acacgacgcg cgttgtacat ctgcggcgcg agaaacacgt cggccatcgt
     9601 caccgcatcc ccatggcaat gccggccgtc gcgcgaatgg cggcggaccg cggcctcgag
     9661 cgccgcgaag cccgtctcga tccagtgccg catccaccgt tccaccgcgc cctccggctg
     9721 cgccagttcg cgacgcaggt agtccacgac gcgcaggttg ttgagcgggt ggatgtcgca
     9781 ggccacgctg agcgccatcg cgcgcacctg agcccgcgcg cgcgcatcgc gcggcagcag
     9841 cgccggctcg ggaatcgtct cgtcgaggta ttcgatgatg gccagtgact gcgtgagcac
     9901 gtcgccaccg gtctcgagcg ccggcaccag gccctgcgga ttcagcgcga gatagtccgg
     9961 gccccgctgt tcgccacgca ccaggtgatg cgccgcctgc tcgcaggcga cgcccttcag
    10021 gttgagcgcg atgcgcacgc gataggcggc gctgctgcgc cagtaaccat gcagtctcat
    10081 gcggactctc cggtcggcag aatcggcgcg catcgccccg aaacaggcgc ggcggcgcgt
    10141 ctgcgcgcgc agtattctgg tacacgcatg ctccccgact ccagcacact gacgctggcc
    10201 cgctgccccg gcctcaccgt cgagcagctc tcggccgccc tcgcgcaggc cggcaacgag
    10261 ccggcgcgcg tgctgcgcct cggcgaagcg cggctgcggg cctgtggctg gcgcgacgcg
    10321 gcgctcgcct gggcgctcgc acccgatgcg gccgaggtcg atctcgatgc cgcatggctc
    10381 gcggccagcg gcgcgcggct cgtgtcgtgt ttcgcagccg actacccgcc gctgctgcgc
    10441 gaggtcaggc acgcgccggc cttcctgtac gtgctcggcg acgtgggcct gctgtcgtcg
    10501 gtgcagctcg cgatcgtggg cagtcgcaat cccaccgcgg gcgggcgggc cgactcgttc
    10561 gagttcgctc gctacctcgc cggcgccggc ctcaccatca cgagcggcct ggccctcggc
    10621 atcgacgccg ccgcgcatcg cggtgcgctc aaggccggcg ggcgcaccgt cgccgtctgc
    10681 ggcgccgggc tcgacctgac ctatccgcgc gagcacgcgg cgctggccgc ggagatcgcc
    10741 gccaccgggg ccctggtgtc ggaattcgcg ccccgcgtgc cgccgctgcc cacgaacttt
    10801 ccgcagcgca accgggtcat cagcggcctc tcgctcgggg tgctggtcgt cgaggcggcc
    10861 cggcgcagcg gctcgctgac caccgcccgc catgccggcg agcaggggcg ggagaccttc
    10921 gcgatccccg gttcgatcca caatccgctt tcccgcggct gccaccagct gatccgcagc
    10981 ggcgccaagc tggtcgaggc ggccagcgac atcctggaag agctgaaaat tcccttttcc
    11041 agacaaacac ttatggcggc cgaggcgccc gccccgggcg cggatgcggc ggcgccacgg
    11101 ttggacaagg attacgaaat cctgttagat gccctcggct tcgacccgac gggcgtcgat
    11161 acgatcgtcg agcggacagg tctggcggcc gattcggtcg cctccatgct cctcatcctc
    11221 gagctcgaag gtcgcattgc cccttacccc ggcggccgct atggccgcct gcagcagaaa
    11281 acatgattac tggcagcgtt ctcgacatcc tcatctacgt cttcgaccat tacatgctcg
    11341 aggacacccc tgacgtgccc gagcgcgagg cgctggcgca ggacctcgaa caggcgggct
    11401 tctcggcgcc caacgtcgag cgtgcgctcg actggctcgc ggacctcgcc ggcgagcgcc
    11461 atcgccccga tttcgcgatg ggcaaacggg cgatccgcgt ctacgccgat ggcgaactcg
    11521 cgcgcatgtc gaccgaatcg cgcggcttcc tgctcacgct cgagcgcacc ggcatcctga
    11581 ccgccgcgca gcgcgaaatc gtgatcgacc gactgctcgc gctcgacacc gacgaactga
    11641 gcatcgacca gctgaagtgg gtcgtgctga tggtcctctc gagccaaccc ggccaggagc
    11701 aggcctacgc gcgcatggaa gatctcgtgt tcgacgccga cggatcagtc ccgcattgaa
    11761 caccggcgct ccgcgcgatc ccaggcgtcc gcggctcgtc cgcggactgt cgtttctggc
    11821 caccacgaat caacgctaac caggcccgca tggcatccaa tctcgtcatc gtcgaatcgc
    11881 ccgccaaagc gcgcacgatc aagaagtacc tcggcaagga tttcgaggtg ctcgcctcct
    11941 acggccacgt gcgcgacctc gtaccgaagg aaggcgcggt cgatccggac aacaagttca
    12001 cgatgcagta tcgcgtgctc gagaagaacg agaagcacgt cgacgccatc gcgcgcgcgc
    12061 tgaagaaagc cgacgcgctg tacctcgcga ccgacccgga ccgcgagggc gaagcgatct
    12121 cctggcacct gagcgagctg ctgcgcgagc gcggcgcgct cgacggcaag gacgtgcatc
    12181 gcgtcgcgtt ctacgaagtg acgaagaacg cgatccgcca ggccatcgag gagccgcgcg
    12241 gcctgtcgat ggacctcgtc aacgcgcagc aggcgcgccg cgcgctcgac tacctcgtgg
    12301 gcttcaacct ctcgccgctg ctgtggaaga aggtgcgtcg cggcctgtcg gccggtcgcg
    12361 tgcagagccc ggcgctgcgc atgatctgcg agcgcgaaga ggagatccag cgcttccagg
    12421 cgcaggaata ctggacgatc gatgccgagg gcgagcacag cgggcagcag ttcccgctga
    12481 agctcgtcga gtacgacggc cgcaaggtcg agcagttcag cttcacgagc ggcgaagtcg
    12541 cccatgcggt cgagcgcgca ctgcgtgccg cggccggcgg ccaggccgaa ggcagcgcgg
    12601 gcacgctcac cgtctcgcgc atcgatcgca agcagcgccg gcgcaatccc gcgccgccgt
    12661 tcacgacgtc cacactgcag caggaagcgg cccgcaagct cggtttctcc gcgcagcgca
    12721 ccatgcgcct cgcccagcag ctctacgagg gcgtcgatta cggcgagggc agcaccggcc
    12781 tcatcaccta catgcgtacc gactcggtgt cgctcgcggc cgaggccgtc accgacatcc
    12841 gcagcgtcat cgcgcagctc tacggcaagg attcgctcgc cgaggacgtg cgcgtctaca
    12901 agaccaagtc gcgcaacgcg caggaagccc acgaagcggt gcgcccgacc tccgcggcca
    12961 tcacgccgca gctgctcgag ggtcgcatcg accccgacca gtggaagctc tactcgctca
    13021 tctggaagcg cgcggtcgcg tgccagatgg cgcacgcgct gttcgatacc gtcgcgatcg
    13081 acatgcaggc cggcgcgcat acgctgcgcg ccaacggctc caccctggtc aagcccggct
    13141 acatcgccgt gtaccaggaa ggccgcgacg atgccgtcga ggacgattcc gaccacgtgc
    13201 tgccgccgat gaaggaaggc gacaccgtga agctcgtcgc gatccatccc gagcagcact
    13261 tcaccgagcc gccgccgcgc tactccgaag cctcgctcgt gaaggcgctg gaagagcacg
    13321 gcatcggccg cccgtcgacc tacgcgtcga tcatctcgac gctcaaggat cgcgagtacg
    13381 tcgagatgga caaccggcgg ttcatcccga ccgacatcgg caagatcgtc aacggcttcc
    13441 tgtcgaagca cttccatcgc tacgtcgaat acggcttcac cgcggcgatg gaggacgagc
    13501 tcgacgcggt gtcgcgcggc gaggaggact ggacgcagcc gctgaagaag ttctggaagc
    13561 cgttcatcga cctcgtcgag cacaccgaga agcatgtctc gcgcgacgaa gtcgcgcagg
    13621 cgcgcgagct cggcaaggac ccggcgagcg gcaagccggt caccgtgcgc atggggcgct
    13681 tcggtccgtt cgtgcagatc ggcaccaagg acgacgagga aaagccgcgt tttgccggcc
    13741 tgcgtcccgg ccagaagatg gacaagatca cctacgagga agcgatggag ctgttcaagc
    13801 tgccgcgcaa cctcggaacc accgccgaag gcgagcccgt gcaggccaac atcggccgct
    13861 tcgggccgta tgtgaagtac ggcgccaagt acgcctcgct gaaggaggac gatccgtaca
    13921 cgatcacgct cgagcgggcg ctcgaggtca tcgcggcaaa gaagctcgcg gacgcgaacc
    13981 gcatcatcca ggacttcggc gttgacgaca tccaggtgct gaacggccgc tacggcccgt
    14041 acatcaccga caagaagaag aacgccaaga tcccgaagga ccgcgatccg aagacgctca
    14101 ccctcgagga gtgtcgcgcg atgctcgcgg cggcgccgga acgcggtgga cgcttcggcc
    14161 ggcgcggcgc cgcggccagg ggcaagaaag cggcagcggc ggccccggcg gccccggcgg
    14221 ccgacgcgcc ggcggaggcg cccgcggcca agaagaaggc cgcgccgaag aaaaaggccg
    14281 ccgccagcaa gaaagccgcc gcaaagaaaa aggcgccggc gaagaagaag gcggcggcgg
    14341 aagcgaagcc ggcctgaggg cgcgcgccga tgaagtatcg gcacgcctac cacgccggca
    14401 acttcgcgga cgtgcacaag cacgtcgcgc tggtcgcgct gctgcgcgcc ctgcagcgca
    14461 aggacaaggg cttcctgtac gtcgacacgc atgccgggcg cgggcgctac gcgctcggcg
    14521 cggggttcgc gcgcgagagc ggtgaggcgc agtccggtgc ggtggcggtg ctcgcggcgc
    14581 tgcgtgacag cgccggcgcg ccggacgaac tcctcgacta cgcggccgtg atcgaggcgt
    14641 ggcgccgcgc cgccgatgcc cgccacgact atcccggctc gccgctcatc gccgcgcaac
    14701 tgctgcgccc gcaggaccac ggcgtcgcga tcgaatcgca gcccgccgaa ttcgaggcgc
    14761 tgcgcaaggc cctcggccgc gcctcgcggg tcgtcgccga gcacggcgac ggctaccagc
    14821 gcctgcgagc cctgctgccg ccgccgtcgc gccgcggtct cgtgctcatc gacccgccgt
    14881 acgaggacac gcggggcgac tttcgcgccg cgatcctcgc cgtacagcac gcactcgccc
    14941 ggttcgaggg ctgcgtgatc gcgctctggt acccggtgaa ggacgcccgc gacaccgacc
    15001 gctggctgca ggaactcgcg cgcggcctgc cccgcccggc gctggcggcc gagctgtggg
    15061 tgcacccgcc cgactcgcgc gcgggcctca acggctcggg cctcgccgtc gtcaatccgc
    15121 cctggctgtt cgccgatcga tgcgacatct ggcagggcgc gctgcgcccg ctgctcgacc
    15181 cggcgggcgc cggcggcaca cgcgtcgtct cgctcgtcgg cgaaagccgg taagctcact
    15241 gcatgggagc cgatccgcag gccgaagtgg attcgtacgg ggtcatcgga cacccggtcg
    15301 cacagagccg ctcaccgttc atccatggcc tgttcgccaa gcagaccggc cagcgcatgg
    15361 tgtaccggct cttcgacgtg accccggacg attttctcaa ccacgtcacc ggcttcttcg
    15421 cgggtggcgg gcgcggactc aatgtcacgg tgccgcacaa gcccgccgcg gccaacttcg
    15481 ccagcacgct gacgccgcgc gccgagcagg cccgcgccgt caacacgctc gcgctgcagg
    15541 cggacggttc ggtgctcggc gacaacaccg acggtgtcgg gctggtgcgc gacctgacca
    15601 tcaacctcgg cctcgtcgtc acgggcttgc gcctgctgat cctgggcgcg ggcggcgcca
    15661 cgcgcggcat cgtcgcgccg ctgctcgatc tgacgcccgc ggagatgctg atcgcaaacc
    15721 gcaacggcga tcgcgcgcgc gtcatcgcgg cgagcttcgc cgatctcggt ccggtcaagg
    15781 gttgcggctt cgacgagatc gacgctcgcc cgtgggacct cgtgatcaac gccaccgcgg
    15841 cgagcctcgc cggcgcgcga cccgaggtct cgccgacgat cattggcccg cacaccgtgt
    15901 gttacgacct cgcctacggc cgcgagccca cgccgttcct gaactgggcg ctggccgagg
    15961 gcgcctcggc cgcgcacctc ggctggggca tgctggtcga gcaggccgcg gagtcgttct
    16021 acctgtggcg cggcgtgcgc cccgacaccc gcccggtgct ggaggtcctg gtgccgggta
    16081 cccggtaatc cggtccttgc gcggctgcgc cgcaatagcc gaatcaccgc aagcccggca
    16141 ccgtcacctc atcgtcacga attcctcggc gctggtcggg tggatcgcca ccgtgtcgtc
    16201 gaagtcgcgc ttggtggcgc ccatcttcac cgccacggcg aacccctgca gcatctcgtc
    16261 ggcgccctcg ccgatcacgt gcagccccac gatgcgctcc tcgggacctg cgaccacgag
    16321 cttcatctcg gcgcgcggct tgcggctcgt cagcgcgtgg tacagcggca cgaaacccga
    16381 ggtgtaggtc ttcaccgacc catcgccgtg gcgcaggcgc gcctcggcct cggtcagccc
    16441 gaccgtgccg atcggcggat gcgtgaaaat cacggtcggc acgttgtcgt aatcgagatg
    16501 acggccggcc atgccgccga acacgcgatc cgacagccgc cgcccggcgg cgatcgcgac
    16561 cggcgtgagc tgcagccgcc ccgtgacatc gccgatcgcg aagacgtgcg gcaggttggt
    16621 ctgctggtac ttgtcgaccg cgatgaaacc gtccgcgccg agggccacgc cggcgcgact
    16681 cagcccgaga ttctccacca tgggttcgcg gcccaccgcc cacagcaggc aatcgaccgg
    16741 gggcagcacg ctgccatcgt cgagcgtgag ctcgagcgcc tcgcccttgc gcgcgagcga
    16801 ggtcggcgtc cggcccgtca cgatactgac acccgaatcg cgcagcgcgc gcatcgcgcc
    16861 ctcgcccaac atgacatcga acgagcgcaa cacacgctcg cggcggatga ccagggtgac
    16921 ctgcgaaccg agcgcggcga ggatgccggc gagctcgacg gcgatatagc cgctgccggc
    16981 gatcagcgcg cgcggcggca gcgcctcgag ctcgaagaaa ccgtcggagt cgatgccgag
    17041 ctcctcgccg ggcagacgga tgcgcgacgg acgcccgccg gtcgcgatca cgatgcgatc
    17101 ggcggcgagg acctgctcgc cgacacggat ctcgtgcgga cccgtgagct gcgcatggcc
    17161 gcgcaccagc gcgacaccgc gcttctcgag gttgcgcgcg tagatatcat tcaggcgcac
    17221 gatataggca tcgcgccgcg ccttgagcgc cgcgaaatcg aggccacgca cgtcgagcgc
    17281 aaagccgtaa tcggcggcgt cgagcagccc cgccgcgagc tcggcggcat tccacatgac
    17341 cttcttcggc acgcagccga cattcacgca ggtgccgccg aggcggccgg attcgatgac
    17401 gacggcacgg gcgccgtact cggcggcgcg ctgcgcgcag gcgagtccgc cgctgccgcc
    17461 gccgatcact gcaaggtcgt atttttcaag catttcgcat tttacacccc ggtacaatag
    17521 agacatgacc aacccgctcc tgaccgacga cctgctgccc agattcgatg ccatccgccc
    17581 cgagcacgtg gcccccgcaa tacgtggcct gctcgccgaa aaccgtgccc gcctcgcggc
    17641 gatcgcggcc gagacgacgc ccaccttcgc cagcgtcgtc gaaccgatgg aagagatgag
    17701 ccatcgtttg cagcgcgcct ggtcgccggt gagccacctc aatgccgtgc tcaattcggc
    17761 gccgctgcgc gagcagtaca acctgtgcct gccgctgctg accgactacc agaccgatct
    17821 cgcgcagagc gagccgctgt acaaagccta tgtggcggtg gcggaacgcg aagcggccac
    17881 gctcgatccg gtgcagcggc gcgtcgtcga ccacatgctg cgcgacttcc gcctggccgg
    17941 cgtggccctc gaacccgccc gcaaggagcg attcaaggcg gtgatgctcg agctgacgac
    18001 cctgcagtcg aagttcgagg agaacgtgct cgatgccacc aacgcctgga gccaccatgt
    18061 caccgacccg gccgagctcg ccgggctcaa cgaaggcgtc gtcgaacagg cgcgcaggcg
    18121 cgcgcacgag aagcgcaccg aaggctggct gctgagcctc gaccagccca cctatgtcgc
    18181 cgtgatcacc gacgcggaat ccgccgcgct gcgccgcgcc tactacgagg cgtggaacac
    18241 gcgcgcctcc gaccagggac cgctcgccgg gcgctggaac aacacgcagg tgatggacga
    18301 catcctgcgc ctgcggcacg aggccgcgac cgtcctcggc ttcgccaact tcgccgacta
    18361 cgccctcgcg acgcgcatgg cgaagagcgt gcccgaagtg atcgacttcc tgcgcgaact
    18421 cgcacgcgcc gcgcgcccgg ccgccgagcg cgaattcgcc gaactcgaga aattcgccgg
    18481 cgcgaagctc gacgcctggg acctcgccta ctggtccgag cgcctgcagc gcagccgttt
    18541 ttccgtttcc caggaagagc tgcggcccta cttcccgctg ccgcgcgtgc tcgcgggcct
    18601 gttcggcgtg gccgagcaac tgttcggcgt gcacatcgcc gagcgcggcg gcgtcaccac
    18661 ctggcacccg gatgcgcgct acttcgaaat caccgacgcg cgcggcgcgc cggtcggcag
    18721 cttctatctc gacccctacg cccgcccgca caagcgcagc ggcgcgtgga tggacgactg
    18781 cgtcggtcgc aaggtgagtg ccagcggcgt caccaacccg gtcgcttacc tcgtctgcaa
    18841 cttcctgccg ccgagcggcg agcagccggc cctgctcacg cacgacgacg tcgtcacgct
    18901 gttccacgaa ttcggccacg gcctgcacca catgctgacc cgcgtcggct acccgagcgt
    18961 cgcgggcatc aacggtgtcg cgtgggatgc cgtcgaactg ccgagccagt tcttcgaaaa
    19021 ctacgcctgg aacgaacagg tgcttggcgc catggccgcg cactacgcca ccggcgcacc
    19081 gctccccgcc gaccgccagc gccagctgat cgccacccgc agcttccacg cgggcctcgc
    19141 gaccatgcgc caggtggaat tctcgctgtt cgacttccgc gtgcacgccg agtacgaccc
    19201 ggcgctcggc ggccgcatcg ccccgatcct tgccgaggtg cgccaggaag tcggggtggt
    19261 gccggtgccc gcgtggaacc gcttcccgca cagcttcggc cacgtgttcg ccggcggcta
    19321 cgcggccggc tactacagct acaagtgggc cgaggtgctc gcggccgatg cctttgcggc
    19381 cttcgaggaa gccggcgtgt tcgatcgcgc caccgcagcg cgcttcctcg actcgatcct
    19441 ctcgcgcggc ggcagccgcg acccgctcga ggcgttcatc gaattccgcg gccgcaagcc
    19501 cgacatcgcg ccgctgctgc gccagcacgg gatcgcggcc tgacctcgat gcgcacgatc
    19561 gcgacctgga acgtgaattc gctgcgggtg cgcctgccgc agctcaccga atggctcgcg
    19621 tcgaatcccg tgcacaccct cgcactgcag gaaaccaagc tcgaggacaa ggcgttcccg
    19681 cgcgcggaga tcgaagcgct cggctaccag gtcgccttca gcgggcagcg cacctacaac
    19741 ggcgtggcga tcctggcgcg tgctccgctc ggcgacctcg tcaccgacat cccgggcttc
    19801 gaagatccgc aacgccgcgt gctcgccgcg accgtcgacg gcgtgcgggt ggtgaatctc
    19861 tacgtgccga acggccaggc cgtcggctcc gagaagtacg cctacaagct gcgctggctc
    19921 gcggccgtgc gcgactggct cgcggccgaa ctcgcgcagc accgccagct catcgtgctc
    19981 ggcgacttca acatcgcgcc cgaagaccgc gatgtgcacg acccggccgc ctgggaaggc
    20041 tcggtgcacg tcagcgaagc ggagcgcgcg gcgctgcgcg agctgctcgc gctcggcctc
    20101 accgacagct ttcgcctctt cgagcaaccg gagggcagct atagctggtg ggactatcgt
    20161 cagggcgcct tccggcgcaa tgcgggcctg cgcatcgacc tgctgctgac atcgaaggcg
    20221 ctcacggagc gctgcaaggc ggcgtcgatc gaccgtgaac cgcgtcgcgc cgagcgaccc
    20281 tcggaccacg cgcccgcctc ggcgagcttt gacatatagg ccgacccccg agcgccgcaa
    20341 agtgtgcggt acgtcgctgg ttttttggcg agtcgctgcc tagactcgcc cgaatacttg
    20401 cggcacctcg gccgatgcac aacaacaggc gcagcgactt cgacgtgacg aacaccgtac
    20461 aggtcagttc gccgggcgac gtttgcgaag cggtcaagaa actgttcacg gccacctggc
    20521 cgggtgcgcc gttcgagccg gtcgcgcgcg gcttcgatcg cctcgaggaa gtcttctccg
    20581 ggcgcgaccc cgaataccac gcgctcgaca ccgtctatca cgacacgcag cacacgctcg
    20641 acatcaccct cgcgatggca cggctgtgcg cgggttacga gcgcgaagcg ccgccgggcc
    20701 gcgcgctcgg cgccgagcgc gcgaccgtcg gcctgctgat cgcgctgttt cacgatgtcg
    20761 gctacctgcg cgagcgcagc gacacggtga cgcagaacgg tgccgaactc accgccaccc
    20821 atgtggcgcg cggcgcgcgc ttcctcgaga aatacctgcc cgccgccggt ttcgaggact
    20881 gggtgccgat cgcgacccag atcatccact tcaccggcta tgaagtgccg ctcgatgcga
    20941 tccaggtgtt cgacgagcgc gaccgcaagc tcggccacct gctcggcacc gccgacatga
    21001 tcgcgcagat ggccgaccgc tgttacctcg agaaatgccg cgaccggctg tatccggaat
    21061 tcgtcctcgg cggctatgcc ctgccgctgt cgaaccaggg cagccgacag gtgaaatacg
    21121 cctcgggcct cgacctgctg cgccagaccc cgcagttcat caccgagacg cgcatgaagc
    21181 gcctggacgg cgcctttgcc tcggcctatc gccacctgga tgcgctgttc gacgggcgca
    21241 acccctacat ggaggcgatc gagcgcaacc tcgtgttcct gaaccagatt ctgcgcagcg
    21301 agagctggcg catgctgcgc cggaacccgc cggtgttcgc cgcgggtgaa gacccgatgg
    21361 gcacgatgcg cggacttgcg atgggctaca tccgccgcgc ctggacgcag taagcgctca
    21421 gcgctcctcg acgtagcgct cgcgcagctg cgcgagtcgc ccgatcaaca cctcctcgcc
    21481 ttccaccccg gcgccttcga tcgcctcgcg cagcacctcg acggtcaatg ccgcgcaggc
    21541 gggccgggcg acgggcgcgt agctcaggtg ccagggctcg ggctgcaccc cgtcgtgggc
    21601 acgggtgtaa ggccgaaaga aaccgaagcg ctgcatcgat tccgcaagcc acgcatcgag
    21661 gcgggcgaaa acgccgcccg gcgcgtactc ctgcggaatc agctgcgtgc ggtagccggc
    21721 aggcagcgcg gcgcgatcga tcacgtcgat gtcggtgccc cagtgatggc ggctcgcgcc
    21781 cggcaacgcc gaccacgcga ggatcgcgtc gatgcgcgca tcgggcgcga gggcgagcgc
    21841 atcgagcggc tcgccgcggc gatcgagcaa cggacgctcg ccgcggtact tcgcgttcca
    21901 gatctcgcgc tgccgctcga aatcgcgaaa cgaggacacc ggcgcgagct cgatgcccgc
    21961 ctcgctgcgc gccgccgcgc acagcgccag cagcggcgcg acgacatcgt ggtgcacgat
    22021 gcagcgcagc tgcggcacct cgatgacatg ggtgcgcgcc cgcccggtca gttcgagcgc
    22081 gttcatgtgc cggtggcgcc ctccgcgacc acccggtcga tgacctgcag gaaagcctcg
    22141 ggctcctgcg cgccggagac cagatagcgg cgcgcgaaca cgaaggcggg cacgccgtgg
    22201 atgccccagg tgtgtgcctg cgcttcgagc gtggcgatct gctggtgcag caacggatcg
    22261 tcgatcgccg tgcgggcggc cgcggcatcg agcccggcct cgccggcgat cgcgacgagc
    22321 acctcgggcg agccgatgtc gcgcccttcg ctgaaatagg cggccagcac ccgttcctgc
    22381 gcgtccgcct gcgccgcggg gcttgccccg cccgcccagg cgatcaatgc gtgcgaacgg
    22441 cgcgtgttcg gcatgcgcgt tatgcgctcg aagtggaaat cgatgccgag cccgggaccg
    22501 agttcgcgca gcgaacgctg ggcgtcggcg aagcggtccg aactgccgaa cttcgcggcc
    22561 aggtactgcg cccggtccgc gccctcgacg ggcatgtccg gattaagttc gaacggcagc
    22621 cagcgcacct cgaccgcgag gtccgcacgc tggccgagcg cctgctcgaa gcggtgcttg
    22681 ccgacataac accagggaca caccgtgtcc gaaaacacct caacgagcac ccggacctcc
    22741 tttcaacgtg attctaggcc gcccgcctcc gggcagggta agatacccga gcttagtaaa
    22801 tacaagggga taagcagtcg ggccgtctac tcggcaccgg ctactgcatc gtgaaccgcc
    22861 gcagcctcag gctgagatac gtggccgtcg ccgttgcggc gatgctggcc gcgaccgtgc
    22921 tgctggccgg cctgctgctg tggcatgcgc gcgcggaatc gcagctggtg tcggccatcg
    22981 ccaccgaaga gcatcgcaac gcgatcggca ccgaactcgc cgcgcgcgcc gaagcgatcg
    23041 ccacgcacgt cgccgatgtc gccgcacccg cggtcgtcgc aggcaatcgg gccacgctcg
    23101 ccgagcgcat ggaggccctg ggcgacgacg ccgcgatcac cgggatcgaa atactggatg
    23161 ccgagggcgc gagtctctac gtgctgcgcc gcgccgcgcc ggacagcgag gcggtgatca
    23221 cccgcagcta cacgcaaccg atccgcgcga tgctgccgat caattccggc acgaccgccg
    23281 cgcagagcgt gggcaccgcg cgcgtcacgc tcaccacacg cgacacctcg ccggccgata
    23341 tcggcgcgcc agtcgccgcc gcgggacgct cgatgctgaa ccgcgcgctg ttcgggctgg
    23401 gcgtgatcct cgtgctcggc ctcgtcagtg cggtggcact cgcctggtgg gccgcgcgcc
    23461 gcatcgagcg gccgatctcc gcgctgatcc gctccgccga tcgcatcggc cagggcgacc
    23521 tgtcgcgccc caccgatctg gcgctccggg acgagctcgg cgagctgcag caggcgctcg
    23581 agcgcatgcg caacaagctg cgccagacca cgatcaccaa gaactacctg aacagcgtgc
    23641 tcaccggcat gaccgacgcg gtcttcgtga cctcgcccga tggggtgatc cgggtcgcga
    23701 accaggccgc gcagcgcctg ctcggctatg gcgaggacga actgaacggc cagagcatcg
    23761 tctcgctgct cgacggcccg gatcgcgaga atttcaacgt actgcaggcc tccaacgaga
    23821 cccgcgaggc tgtggtgcgc acccgcaagg gccagacgat ccccgtatcg ctcaccggtt
    23881 cggccatcgc caccgacgac ccgcagttcc agggctgcat cttcgtcgcg cgcaacatca
    23941 ccgacaggaa gcgcgccgag cggcgcatcc gctacctcgc ccgctacgac gcactcacca
    24001 aagtgctgaa ccggatgcag ttccagcacc tgttgcagca ggcgatcgcg cgcgcgctgc
    24061 gcaacaacca gacggtggcg ctgctgtacc tcgacgtcga tcacttcaag gaagtgaacg
    24121 acaccttcgg ccacgccgcg ggcgatcgca cgctcgagat cctcaccgag cgcctcacgc
    24181 gcgcgctgcc caaggactcg gtggtcggcc ggctcgccgg cgacgaattc gcggtgctga
    24241 tcgacggcct gtcgggcgat gtcgatcatc gcgggccgat cggccagctc gcccgctcga
    24301 tcctcgccga gaccgacaag atcttccagc tcggccagca cgagctcttt ctgaccacca
    24361 gcatcggcat cgccttctgc ccgcgcgacg ccgagaacgt gatcgacctc atcaggaacg
    24421 ccgacgcggc gatgtactac tcgaagcaga acggcggcaa caccttcgcc ttctacgccc
    24481 cggaaatgaa cgccgcctcg gtcgagcgac tgatgctgaa gagcaagctg cgtcgcgcgc
    24541 tcgagcgcga cgagctgatc gtgaactacc agccgaagat cgacctgcgc accggccgca
    24601 tcgtcggcgc cgaggggctg ctgcgctggc gcctgccggg ccacggcgac atcccgccga
    24661 gccagttcat cccgctcgcc gaggaatcga acctgatcct cgagatcggc gagtgggtgc
    24721 tgaaccgcgt ctgcctcgac taccgcacac tgcgccgcga agtcgccgat ccgggacgca
    24781 tctcgctcaa cctgtcgctg aagcagctgc gccaggcctc gttcatcgtg cgttgccgct
    24841 cggtgttccg ccgccacggc gtgtcgccct cggcgttcga actcgagatc accgaatcga
    24901 ccctgatggc cgacgccaaa cgcaccgtgc acctgctggg agagctgcac gccatgggcc
    24961 tgcacctgtc gatcgacgac ttcggcaccg gctactcctc gctctcggcg ctgcagcagt
    25021 tcccgatcgg cacgctcaag atcgaccagt cgttcgtgca gaatgtctcg acccatccgg
    25081 gcgatgccac gatcgtgcgc accatcatcg acatgggtcg cagcctcggc atggacgtga
    25141 tcgccgaagg cgtcgagacg accgcccagc tcgaattcct gcgcgatcac cactgcgatt
    25201 acggccaggg ccggctgttc ggtgacacgc tgccgctcga ggatctgctc gagctgctga
    25261 tgcagcagca gaacggcacg ccgccgttcg cggcgttgat tccgcccgcg gcccggtccg
    25321 cgggcctgag cgcctgaccc gccccatgcg ccatgctgac gctacgcgac gttagtctgc
    25381 gccgcggtcc gcgcgtactg ttcgaacagg cttcggtcgg tgtgttccgc ggcgacaagg
    25441 tgggcatcgt cgggcgcaac ggctcgggca aatcgagcct gctcgcgctg atccgcggcg
    25501 aactcgcgcc cgacgtcggc gactatgacg ctcccgcgaa cctgtgcatc gccatcgtcg
    25561 agcagtcggt accggactcg gcgcagagcg tcgtcgacta cgtgcgcgag ggcgacaccg
    25621 aactgatccg catcgaacac gcgatcgccg ccgcgcacgc ggccggcgac gggcaccgcg
    25681 aagcgcagct gcatgcggac tacgacgccg cgggcggcta cggcgcgcgc agccgggcgg
    25741 cctcgctcgc gaccggcctc ggcttcgagg ccgacgacat cgagcgcccg ctgcacgtgt
    25801 tctccggcgg cctgcgcatg cgcgccgcac tcgcgcgcgc gctgatgcgc cgctccgaca
    25861 tcctgctgct cgacgagccc accaaccacc tcgacctcga cgcggtgctg tggctcgagg
    25921 gctggctgcg cgcttaccgc ggcacgctgc tgatcgtctc gcacgatcgc gaattcctcg
    25981 acggcgtggt gtcgcggatc ctgcacatcg agggcggccg gctgaccgcc tacaacggca
    26041 actactcctc gttcgagcgc cagtacgcgg cggcgcgcga gcgcaatgcc gcgctcgccg
    26101 aaagccagcg gcgcgagatc gcgcacgtcg agagcttcgt cgagcgcttc cgcgccaagg
    26161 cgagcaaggc ccgccaggtg cagagccgca tcaaatggct cgaacgcctg ccggccatca
    26221 tcgaagccca gggcgaggaa gcattcgcct gggaattccc ggcgccggcg aaactgccgc
    26281 ggccgctcgt cgcgctcgac cgggtcagcg cgggctacgg cggtcgcgcg gtgttcacgg
    26341 gcctcggcct gtcggtggcg ccgggcgatc gcatcggcgt gctcggccgc aatggcgccg
    26401 gcaaatccac gctgatgaag accgtggccg gcgccctcgc gccgctcgcg ggcgaacgcg
    26461 tcgcctcgcc cgacctcgaa gtcggcttct tcgcgcagct cgaagtggag cagctcgacg
    26521 cggccagctc ctcgatcgag gaactggcac gccgcggcgg cccccaggtc gccgcatgga
    26581 cgccgcagca gcagcgcgac catctcggcc gcttcggctt tcgcggcgat cgcgtcttcg
    26641 aggcggtgcg caacttttcg ggcggcgagc gggcgcggct cacactcgcg atcatggtcg
    26701 cgcggcgccc caacctgctg ctgctcgatg agcccaccaa tcatctcgat ttcgagatgc
    26761 gtaacgcatt gttgctcgcg ctgcaggact acgctggcgc cgtgctgatc gtgtcacacg
    26821 accggggcct gctgcgtggc gcctgcgacc gcttcatggt ggtcgcgggc ggctcggtgc
    26881 gcccgttcga cggcgacctc gaagactatg ccgcctggct cgccaaggga gcgcccaccg
    26941 gcgacggcaa agcgtcgggc cgcgccggcg acagtcgcga cggcgccgag cgcacagcgc
    27001 agccccagcg ccgcgaccgt gacacgcgcc ggcgcgaagc cgcggcgcgc aaccggctct
    27061 cgccgctgcg catcgaactg cgcagcatcg aagacgagct ggcgcagctc gcgacccagc
    27121 gcgcggcgct cgaacgcgaa ctcgccgacg acccgacgaa atacgcgctc gcgaccgact
    27181 acgagcgcct cgccgcgcgc accaccagcc tcgaagagcg ctggctcgaa ctgagcgaag
    27241 agatcgatgc cgcccagcgc gcgtaagcgc tggctgcgct ggaccggcgg cgcgctgctc
    27301 gccgtctggc tcggcgtcat ggcctggaac acctggaagc cgctgcccga aggcgtcacc
    27361 gtggaaagcg gctggagccc actgcaggag ggcgacgtgc aattcctgcg tgacatcacc
    27421 gcggccgacg cctacggcca cccgatcgtc gatcagcaga tcttcgaggc cgtcttcgaa
    27481 ctgatcgccg aggcgcggca cttcatcctg atcgacatgt tcctgttcaa cgcgcatcgc
    27541 ggcgccgatg tcgccgcgcc cgttcaccgt acgctcgccg ccgaactcac gaacgccctg
    27601 ctcgaacgcc gccgcgccga ccccggactg cggatcgtgt tcatcaccga cccgatcaac
    27661 gacgtctacg gtggcgcgcc ctcgcccgaa ctgcagacgc tgcgcgacgc cggcgtcgaa
    27721 gtggtcgtga ccgacctcga ccgactgcgc gactcgaacc cgctgtactc cgccttctgg
    27781 cgcatcgccc tgcgctggtg ggcgcgcggg caacccggca cctggcccga ccccttcgat
    27841 gcccagggac cgggtgtatc gatgggcgcc tgggcgcggc tcgtgaactt caaggccaac
    27901 catcgcaaag tgctgatcgt cggacgcgaa gacggcacgc tcgccggaat cgtcacctcc
    27961 gcgaacgcgc acgacgcgag cagcagccac tcgaacgtcg cgctgcgcat cagcggcccg
    28021 gtactcgaac cgctcatcgc cagtgaactc gcgatcgcgc gctactcggg ctgggagggc
    28081 gacccgctcc cggtcccggg gaccgcgccg acggccgcaa cggagactgc ttcggcgacc
    28141 ccgtcgagcc cttccctgcc gggccgcgcg cgcatgctca ccgaatcggc gatcgccgac
    28201 gccgtcgtcg cgcgcctgca gacctgcgtg gcgggagacg cgatcgacgt cgcgatgttc
    28261 tacctctcgg accgcagggt actcggcgcg ctgcgcgatg cagccacgcg cggcgcggcc
    28321 gtgcgcgtgc tgctcgatcc gaacaaggac gccttcggcc gccagaagaa cggcatcccg
    28381 aaccgcccgg tggccaccga actcgtcgcg gcgaccgacg gcgcgatacg cctgcgctgg
    28441 tatcgcaccc acggcgagca gttccacgtg aaattgctcg cgatccgcgg gcgcgacgac
    28501 ttctggctga cgctcggctc ggcgaacttc acccgccgca acgtgcgcga ctacaacctc
    28561 gaagcgaaca tcgccatcga gctgccgcgc gactccgcgc tcgagctcgc ggtgctcgaa
    28621 tggttcgacg gactctggat gaaccgcgcc ccggccggcg tcgagtacac cgccgaatac
    28681 ggcacctacg ccgacccgcg ccagatccgc tactggggct atcgggtgat ggaggcgacg
    28741 gggttgtcga cgttttgaga gcgcttcctg gcgctcgccg atcattcagg tcctggagcg
    28801 acttcggcgg gggcgggaat aggccgcagg cgccggctct ttgatcaaca gccctgccgg
    28861 aacttccaac cgctcgtaga gggatcgaat catgggcaag gtcagcggac gcttgcgcgc
    28921 gaggacttcc gatgcccggt tcttgccgcc gagtatcacc gcgagatcct tctgtcgaag
    28981 cccctgctgc tccatgcgaa agagaatcgc gtcgaccgga tccggcttct cgaagggaaa
    29041 ccgcgtcttc tcgtaatcct ccacgagctt cgccagcagc tcgaggcgga tgcctttctt
    29101 cgactccggg tccggatctc cggcggcgag cgcttctact tccgagagga agcgacggta
    29161 ctgcttgtct gttttgatga ttctgggttc cacgccggtg ctccttactc tcacggtttc
    29221 gcattgcgcc ggtcatatgc agcgtgcgta ccgatccaat tcacgacaac cacgccagcc
    29281 cgataggcca cttgcacctc gagccggtac gcattcccct tcacattgaa gatgactgtg
    29341 ttgcttccga caaagcttgc gctcgaatag cgatctttga tggtctgcgg cgtgtcccac
    29401 gaaccagact ccgtttcggc gagccaggcc acgacccaat tgcgcgcatc cgcatgtctc
    29461 aagcagaacc gggcaagagt cttacgcccg accacgcgca tggaggctgt ccgatctcaa
    29521 agttaccggg aactctgtca ggtaagttcc catagtggga acctggtgtc aaacttcaaa
    29581 ggtggggtct tcgtcaccga tgggacatcg cgactcaaca gcggggggag atcgagcgca
    29641 tagatgacgg ccgctacgat cacctgccgt ggcctcgcta cgcccggtca gacccgccta
    29701 tatcaaccag aaccgctccg gcgccaccgc cagccatgcg caacacgttc tcacgatgaa
    29761 actccaggta ttgctgctgg ccctgtgaga acgcgcctac gttcaagttg gcttgcgggt
    29821 tcaagcccat tcgcttgagc gacaccgggt ccgctacagg cgacacaata agctggccag
    29881 agttttcaaa cgagatgaat cccttgtcga acagatgatc aatcgttggc gtcaaaagca
    29941 aaccgttctc gccatcaagc cgctgttcgt tgctactgtc gcgccaaggc tgaatgtggc
    30001 tggcaatcag atgctccagg cgctccaccc gtgtgacgcg acaggcacgc tcgatcgacc
    30061 gcacgttctc cctgaatagc ccctggccgc ggcgcgcttg cacgagcgct gttctctcgg
    30121 tctccggaat ttcggcactc gcggcaatct cgacctcgag gcgtcgctcc cactcttcga
    30181 tgtcgcgctc aggtgccggg gaatcgcgct cgacttcact cacttggcgc ccggtgtcgg
    30241 ccacctgata cgcctcggaa ccaatcaggc cgaaaagcgt cgacgcaaac ccttggttga
    30301 tctcagtcag gtagacggct tgaagaccat ttccgtctga tctcaaaggc gcataccttt
    30361 ccgccaaata cggtcgcaat cttgctatgt gatcccttgg acagatcgag ctcgaaagtc
    30421 gttgccaatg tacaccgacc cgccagccta tctggctcca gttcgtaccc gcgttaccga
    30481 actcttcggg tttcggactc tcccggcagt agccgctgac tatgccgagc gctgaaattc
    30541 gcgtgtcata aaatgaaaag acgatgtcgc caggcgcgac ctcgcgcatg aactcgtaga
    30601 acggattgcg gcggccattc ttgctacgct tgggcgacca gagatacgcc ctttctgagt
    30661 gagcgcggcg gattgacttc gaccgcgcta gtcaagcgcg ccacttccca cccatctggg
    30721 atctcaccga cgtcggagtc gacaaggcgg gcggggaaga ggtcggcgag gggcttgggg
    30781 aggcctgtgt cgcggccttc ggctttggcg cggacggggt ggtagtccac gaaccacgac
    30841 ttgaagagcg cgcgagccat cgcctccagt gtctcgccca tccgccggtt cagctcgatc
    30901 ttgtcgtcga gcgtgccgag gatgtgggca atggcgcgtt gttcggaaag cggtggaacg
    30961 gacagtggaa acgaccggat gatcccctcg ttgaggttcg gcatcgttgc gccgatcgca
    31021 tggaatttga accactgaac cgaggatgga tccgcgagaa cgtgcgagag gtaggcgggg
    31081 tcaatcgcgt cgctgtcgcg ggcgactcgc aggcgtatcg ctcctgtgcc gcaaatgaac
    31141 ccgtcttcgg ctttgcgaac gcaaccgatg tgcccggcgg cttgcactcc gcgccgagcg
    31201 aaaagaatgt cgcccggtcg gagccgatgc cgctcgagtt ccttcgcttt ggtctgcgtg
    31261 atcttcggga gaaccgaatg atcaatctgt ctagctcgaa tagcttcggt cggtactaca
    31321 gggacaccgt cgtccacgta gtcgtacgcg tggagctgag tgccaaacgg tcctgtctgc
    31381 agttcagcat ctccggcgtc gcacagtgcg ccgatcgaag ccttcctcca atcatccgcc
    31441 atacccaagc tccctcaggt tggcggcaat tgcagcatca agctttgcgg cctcggcctg
    31501 ctgttcgcgc agcgtcgcgg tgagccgccg catcttctcg tcgaacggct cgccgtcgtc
    31561 ctcttgtgcc tcggcgccga cgtagcggcc aggcgtgagc aaatggccgt gcttgcgcac
    31621 gtcgtccagt ggcgcgctct tgcagaagcc gggcacgtcg gcatactcac ccgcccctgt
    31681 ctctccgcgc caggcgtggt aggtatcggc gatgcgggcg atgtcctcgt cggcaagctc
    31741 gcggtgggtg cgatccacta ggcgcccgag cttgcgcgcg tcgacgaaga gcatctggcc
    31801 gcggcggtcc cgaaacttgc cgttcttgcg gtcgcgtgca aggaaccaca ggcacgcggg
    31861 gataggcgtc gagtagaaga gctggcccgg cagcgcgacc atgcagtcca cgaggtcggc
    31921 ctcaatcaga ttctttcgga tttcgccctc gccggactga ttggacgaca tcgagccgtt
    31981 ggccagcacg aagccggcca cgcccgtggg cgcaaggtgg tgcacgatgt gctgcaccca
    32041 ggcgaaattg gcgttgccca ccggcggcac gccgtattgc cagcgcttgt cgtcgcgcag
    32101 gcgctcgccg ccccagtcgg agatgttgaa gggcgggttg gcgagaatga agtcagcctt
    32161 caggtccggg tggcggtcgt tgtggaaggt gtcgccctgg gcgatctgcc gctgtcgatg
    32221 ccgcggatgg cgagattcat gcgcgccagg cgccaagtgg tgtagttcga ctcttggccg
    32281 taaattgaga tgtcggcctt ggccttggag ccggtgtttc cgccgtggcc gttgcccttg
    32341 gcatgcgcac ggatgaactc caccgactgg acgaacatgc ccgaggagcc gcagcaaggg
    32401 tcgtacaccc ggccgcggta gggctcgagc atctcgacca gcagcttgac cacgcagcgc
    32461 ggcgtgtaga actcgccgcc cttcttgccc tcggcgctgg cgaattgaga gaggaagtac
    32521 tcgtagacgc ggccgagcac atctttggcg cggctggcct ggtcgcccac cttgatgttg
    32581 ctaatcatgt cgatcaactg gcccaggcgc tgcttgtcga gcgcggggcg ggcgtagtcc
    32641 ttgggcagca cgcccttgag cgccgggttg tcgcgctcga tgccggccat agcatcgtcg
    32701 acgagctgcc cgatcgtgct ctggcgagcc tgtgccttca ggtgggccca gcgcgcctcg
    32761 ggcggcaccc agaagatgct gcgggcacgg tactcgtcgg ggtcttcggg atcggcgcct
    32821 tgggccttct ccgccagcag cgcggcgtgc ttttcctcaa acgcatcgga gatgtacttc
    32881 agaaacagca gcccgaggca aacgtgcttg tattcggcgg cgtccatcga gccgcgcagg
    32941 gcgtcggcca tctgccagag ctgggctttg tatccgaggg gtgcggtggt ggaggtaatt
    33001 ttcttgcgtg atttggccat tcaactgcct gtgtcggcgc ctaagctccc cgatagagtg
    33061 cccttgtgat tctagggatg agggcattct tgactgtgcg cgagttcgcc tattggatca
    33121 aggggttgta ccgattgtga catgtccggc agcatgattc cggaatgagc agttatatga
    33181 aagtgtgact acttttctca cttcccccgg gggtagcggc actggtgcca cgccgggcga
    33241 gcgccgcttc gccgagcgcc tcggcgcgct gctcgaggac gactaccttt gctggtacga
    33301 cgttcctgtc gggccgcggc accagcatcc ggacttcctg ctcctccacc ccggccgcgg
    33361 tttgctggtc ctggaggtga aggactggaa actggacaac atccatgagc tgaccaccca
    33421 gagcgtgacg ctcaatctgg gctcggggct gaagaccaag gccaatccgc tggaacaggc
    33481 gcggcattac gctcatgccg tcgtgaaagt tctggagcgg gatccccggc tgctggttcc
    33541 acccggcgag aaatacgagg gtcatctgcg ctttccctac ggctacggcg tggtgctcac
    33601 gaacatcacc cgcctgcagt tcgagcgctc cggcatggcc gagacgattc cgccgcacag
    33661 ggtcatctgc cgcgatgaaa tgaccgagtc ggccgatgcc gagacattcc agaagcggct
    33721 gtgggatatg ttctccgtcc gcttccagag ccagctcacc cttccggagg tcgagcggat
    33781 ccgctggctg ctgtttccgg agattcgcat tcaccagcaa ggcctgccca tcgaggccca
    33841 ggccgacgac gcacgtgcgc ccgattcgct gcaggtgatg gacctcgcgc aggaggccat
    33901 cgcacgcggg ctcggcgacg gacatcgcgt cattcacggt gtggccggat ccggaaagac
    33961 cctgattctg gcctatcgct gccagtacct tgcggccacg ctcgccaggc ccatcctcgt
    34021 gctggtctat aacaaggcgc tcgcggcctg gctgcagcac cagatggcac tgcgcggact
    34081 gtccgaccgg gtcacggtgc ggaacttcca tggctggtgc cacgacatgc tgcggctgta
    34141 tcacgtgccg aagcccgccg atggcaagga cttctatccc gcgctcgtga acgcagtgat
    34201 ccgggcggtc gatcgcggcc agattccgcg cgcccagtac ggtgctctgc tgatcgacga
    34261 gggtcacgac ttcgaacccg actggctgcg ccttgccgtg cagatgctcg acccggagag
    34321 caaccagctg ctgttgctct acgacgatgc gcaatcgatc tacggcgcga agcgaaaagg
    34381 cttcactttc cgcagcgtgg gtgtgtccgc ggtcggcagg acgaagattc tcaagcgaaa
    34441 ctatcgcaac accaacgaga tcctcgcctg cgcggccgag ttcgcgcgcg aattgctgca
    34501 ggaggttgat tcggacgagg atggtgtacc gctggtctca cccgtatccg gcgggcgaag
    34561 cggcccgcca ccgaagttct acgatgcgcg cacgctcgaa caggaggcgg cctgcatcgc
    34621 acgccagctg cagcgcaggc accagtccgg cacgccatgg cgcgagatgg gcgtcttcta
    34681 ttccgcgccc ttcgtggccg agacggttgc gaacagcctc accgatgccg gtattccgta
    34741 cgattggctg aaggacagca gctccaagaa gtgggatccg gataaggaca gcgtcaagct
    34801 gatgacgatc cacagcagca aggggttgca gtaccaggtc ggcatcatcg ccggcgc
//
LOCUS       DAOQVY010000007        34759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_1428_length_34759_cov_5.974527, whole genome shotgun sequence.
ACCESSION   DAOQVY010000007 DAOQVY010000000
VERSION     DAOQVY010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 34759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34759
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1428_length_34759_cov_5.974527"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKN91_01465"
     CDS             complement(<1..194)
                     /locus_tag="PKN91_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26766.1"
                     /translation="MNALAFVIVAIAACSVLWQLVPARARYKALRALQAALPQRGYAA
                     RIGHGIIAPLVRRAAKGLGGG"
     gene            complement(191..2092)
                     /locus_tag="PKN91_01470"
     CDS             complement(191..2092)
                     /locus_tag="PKN91_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HNS26767.1"
                     /translation="MDAANRALADLRIALVGVPNCGKTALFNRLTGSHQKVANYPGVT
                     IERKEGQFHNRAGRRFAVVDLPGTYSFTPASLDEAITRDVVLGRIAGEPQPDVIVCVA
                     DATHMPLSLRLILDLQRLGRPMIVALNRSDIAKRRGFTLDVAKLADKLGVPIVETVAI
                     APGGTDQLIEALGTFEAAAVLHDAVPAVDGAAHAIAAPDRAEVERTQQLVPRLLAEVG
                     YGEPLRARAAARADAILMHPFGGMLVLGTVLFFVFQAVFSWAAVPMGLIEAGVGAIGT
                     WVSGALPDNALRSLLVDGIIAGVGSVLVFLPQILILFFFILALEDSGYLPRAAYLLDK
                     LMGSVGLSGRAFIPLLSSFACAIPGIMAARTIPSVRDRLVTIMIAPLMTCSARLPVYA
                     LLIGAFIPAAAAGPFNLQGLVLFALYAAGVLSALGVAWFMKHFAGKQNHHPLMLELPD
                     YQWPDPRSLLVGLWDRARIFLRRVGGVILSLMIILWFLSSYPAPPPDATGPAIEYSFA
                     GMLGGALHTIFAPIGFNWQISIALVPGLAAREVAIGALGTVYAMSATEGALETAIMPV
                     IASGWSLATGLSLLAWYVFAPQCLPTLAVVKRETNSWRYPLIMAGYLFALAYLASFIT
                     YRVALALTT"
     gene            complement(2094..2384)
                     /locus_tag="PKN91_01475"
     CDS             complement(2094..2384)
                     /locus_tag="PKN91_01475"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNS26768.1"
                     /translation="MTESPGGPVPMSTLMRGARATVHRVLDDTLSVGDALHSTVGQRL
                     VELGFVQGAEVEVVESMWPFGDPLAVRVRGSTFALRRGEAAAVMVVPANGAG"
     gene            complement(2477..4681)
                     /locus_tag="PKN91_01480"
     CDS             complement(2477..4681)
                     /locus_tag="PKN91_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HNS26769.1"
                     /translation="MSTPTFARPASLLLAVAAAPLAAQTVDLEVVTITATRIETRVVD
                     VPATVTVKGREDMDRELVFDLKDLVRYEPGVTVSDDGGRFGLSDITIRGIGGNRVLME
                     VDGVRMPDAFSIGSYADAGRDVVDTDLLKRVEIVRGSVSSLYGSDAIGGVVAFTTKDP
                     ADLLSADRNYGFSTKAGYSGRNDSTVAGGTFAARAGAFSGLLAYTRRDGHETENSGDI
                     GGVGTMRTEPVPQDRYSDSVLAKLVFDATDTQRLRLTWEGSRIDTFTDVLTAVSATPP
                     VAGGTQTTGLVGSDEQQRSRITFDHAFPLGEWRVYRQESETSQETLESRNAATLAGVI
                     TPQTRWRLFDFEQEEVGAEVTLFSRFEALGAAHTLAYGAEYIETDTVQLRTGLQTNLL
                     TGVTSPAILPDVFPVRDFPLTKTRRASIFVQDEISLGAWTITPGVRYDDYALRPTVDA
                     IFAADNPGFVAADIDETNVSPKLGVMYRITEYSTGYFNYAAGFRTPPYDDANFGFENL
                     AFGYTAIANPDLKSETSDSFELGWRLARPDGHYLALSAYYNLYDDFIESLVGSLDPAR
                     GLIVFQSQNLNEVRIRGVEVAAGMPLEGQSDSLRGLSVRLAASYARGENRTDDTPLNS
                     IDPPKAVIGFAWRAPSQRWGAELVTTFADSPRRVDDSLGEPFVPPSYTKVDLLADIAI
                     TPQLRLNAGLFNLTDEKYWEWADVSGRLASNRWLDRYTRPGRTAGATIRYEW"
     gene            4812..5453
                     /locus_tag="PKN91_01485"
     CDS             4812..5453
                     /locus_tag="PKN91_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNS26770.1"
                     /translation="MSRIAERRQEEKERRHSDIIDAAEAVAATVGLDAMTMDEVARKA
                     RLSRALLYVYFKDKPDLLFAICQRALENLRVRFEAVAHSHATGLAAIRAMGTSFVEYS
                     REQPVRYDALARFARHAPQQADGAANERACLETGDSVHRIMDACIERGIADGSIRADV
                     GPPGLVALSLWSYTLGIIQLATTKAAVIEHRGYDVEHLIEHALQMAARALAPR"
     gene            5471..6865
                     /locus_tag="PKN91_01490"
     CDS             5471..6865
                     /locus_tag="PKN91_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNS26771.1"
                     /translation="MPARAATSVRRRLRAAGCALCLLALALPFAGAVAARSLTEVVDE
                     YVREGLRANLALRSQAFDVERAIAALDGARAGYLPTLGLDARYTRSEGGREFQVPLSQ
                     LDPMLTDQTIPFLREREQDTRLTLLQPLYAPAIPASVRAQRSRLAASEYAQLALARRL
                     QRDITVGYLDWLRATKTVAIVEASRVLLDENLRVNDSLFRNGKVTRDQVLRAHAELLE
                     VQQQLREAQNLAQQARSYLNFLLNRPLDTALEATEVDEVVARSAADFATLAAAALERR
                     PELAQAESAVQAAEAQIGVERAQLKPTLGIGVDGGIQGEDYGTGHGYNYGQASLRLTW
                     KLFDGGANRAAVRSARAYARRTATERDELAQQIQLEVQQALDRLHTAGDSLATAEARS
                     EAARAGFRIASRKRDEGVISQVEFIDARSTLTGAELNLNLTRFALLARQAELDYVTAA
                     GLLPVDPASTGSAP"
     gene            6862..7953
                     /locus_tag="PKN91_01495"
     CDS             6862..7953
                     /locus_tag="PKN91_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNS26772.1"
                     /translation="MTRQVRPGWLLLLALALAACSDSAPSPQPPATPVRVAVAHAGPA
                     VAPFATNGIVAAKDEMRLSFKIGGIVRRIAVTEGERIARGQLLAELELTEIDAQLEQV
                     AQLAAKAQRDLARGERLYADQVITLEQLQDLRTQASIAQAQATGARFDRGHAVITAPH
                     DGVVLRKYVQEREIVGAGAPVLMVSGAERGHVVRAALADREIVQLRLGDTADIRLDAY
                     PGRRFSGTVTEISGAAETGSGLFPIEVRFGEDAPQLASGLVAKLTIHPAAPGATNLTY
                     VPIAAIVEGDGERASVYVLREGRAERRAVTVAFITHDGVALSAGVASGETVVTDGALY
                     LADGEQIRVVGDAAETIGAIALPGRAAES"
     gene            7950..11033
                     /locus_tag="PKN91_01500"
     CDS             7950..11033
                     /locus_tag="PKN91_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNS26773.1"
                     /translation="MTALEFPIRRYQFTLVAFLCLIALGWYAFANVPREEDPYFKIPG
                     FFVTAIYPGADPRDLERLVAKPIEDRLAELDDVKKIETTITDGVSFTAIEFQARVDPD
                     EAYDEVMREVNALRPTLPQEIAEIEIRKLSPGLVNTVQLALVSEEATWRELEDYARDL
                     KDTLKPVTGIRTAESWAIPRRELRVQLDLKRMAEIGVTPAQVAQAIASENANIPAGTI
                     DIGPRGFSLKTSGAYETLDEVRDTVVTAGGGHAVRIRDIAAVSWTTAPFSHVGRYDGR
                     RAVFVTANQKEGYNILEVQQGIDAALDRFEAGLPQRITLERGFQQSKNVEQRLGRLYT
                     DFAIAIALVMLTLVPLGWRAAGIVMVAIPLSLAFGIAILYLIGYSLNQISIAGCVVAL
                     GLLVDDSIVVVENIARHLRMGLDRTAAALAGTRQIFVAILGCTATLIFAFLPLVMLPG
                     TAGKFIRVLPVTVIATITGSLIVALFVIPFLASRLLPRDAAQSGNPALQRVMGAIHRY
                     YRPALHYCLARPRATVLVSIGGSLLLSAALVPLIGSSLFPKADTPQFLIQVEAPAGAS
                     LEATDEALRFIEAKLRSTPAVKGWFTNVGHGNPQIYYNHITRRDAANFGEVFVQLTHY
                     DTHETPRTLEALRTALRDYPAARIYVKEFVNGPPISAPIAVRVVGRDLEAIEALAGQV
                     QRLIEATPGTRDVQNPLEVARTNLRLAVDAQKANLLGVPTIEFDRAVRLSVEGLPAGK
                     FKDPDGEQYDITVRTAVGTRADLASLGEVRVPSLSGALLPLSQLATLQFEKAPVVIQR
                     YDRERSVTIDADVQAGYNTAKVTADVRRQLDAMDWPRGYHYKLGGEAESSQEAFGGIG
                     IAVIVAIFGIFAILVLEFGNFKSTLIVLTVVPLGVLGGLLMLLATGNSISFTASIGFI
                     ALIGIEIKNSILLVDFTNQLRADGVALDEAIEQAGEIRFLPILLTTLTAIGGLLPLAV
                     QNIGLYSPMAWVIIGGLISSTLLARLVTPVMYKLAPPAIAPAGQPIRPSTRPMTV"
     gene            complement(10994..11389)
                     /locus_tag="PKN91_01505"
     CDS             complement(10994..11389)
                     /locus_tag="PKN91_01505"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HNS26774.1"
                     /translation="MEINWLAVAVAAIASFLVGAVWYSPLLFAKAWQREIGISDTDLA
                     KGNMGRILGSAFVLTLLSTLVFHMFLGPSPGVQFGAGAGFAAGLFWVAFGKGVDYLFE
                     RRSLRHFLINGGHHTVAYTVMGLVLGLMG"
     gene            11518..12483
                     /locus_tag="PKN91_01510"
     CDS             11518..12483
                     /locus_tag="PKN91_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNS26775.1"
                     /translation="MFLDYATVSANDLDASGLARACPNLEFYDVTPPGEVAGRIAGAA
                     ILLVNKARITRELIEATPTLRLIALAATGTDNVDLAAAREHDVAVCNIRDYCTPSVVQ
                     HVLGAILALTHRYADYSRIVTDGHWGESPQFTMLDFPIRELGGRTLGVVGYGTLGRAV
                     ARACEQALGMRVLVARREGGPGRVGEADTDARVPLDALLRVADVLTLHCPLTPATRGM
                     IGAAQLAMMKPDAVIVNTARGALIDVQALADALRAGRLGGAAIDVLPEEPPVSGSPLF
                     APDLPNLIVTPHTAWAARESRQRALDRMAENVADFLAGGQLGRVV"
     gene            complement(12516..12956)
                     /locus_tag="PKN91_01515"
     CDS             complement(12516..12956)
                     /locus_tag="PKN91_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNS26776.1"
                     /translation="MNANRAPRLSAGVVVVRRDGDSWLFLLLRAYRSWDFPKGMVEAG
                     EEPHAAARREVREESAIDDLEFTWGDLYRDTGPYTRNKVARYYIAQTHTARVTLPVNA
                     DLGMPEHHEWRWVTFDEALDLVSPRVAPVLEWARGIVLGLGTHD"
     gene            complement(12972..13505)
                     /locus_tag="PKN91_01520"
     CDS             complement(12972..13505)
                     /locus_tag="PKN91_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNS26777.1"
                     /translation="MSLDTIRDAIPDYARDLKLNLGSVLTTQGAPGLSVKQLWSVALA
                     TAVAARNPAFARDIEALAREHLDDADVNGARAAAAIMGMNNIYYRFLHLVEDGEYQSM
                     PARLRMNVIGNPGIDKLDFELLSLAISAVNGCGLCIQSHEKKLREHGVTRETIQSSVR
                     IAAVIHAVAGVLESAQG"
     gene            complement(13599..14138)
                     /locus_tag="PKN91_01525"
     CDS             complement(13599..14138)
                     /locus_tag="PKN91_01525"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNS26778.1"
                     /translation="MLGIGQKFPQYSLTGVVSNDVATAFKPFTNDSFTGKWQVVFFWP
                     KDFTFVCPTEIAAFGKLDKEFKARDAQLLGVSIDNEFVHLAWRREKEELKDLPFPMLS
                     DLKRELSGELGIIDPAVGVAQRATYIVDPEGVIRFVYVTDLDVGRSPDEVLRVLDALQ
                     TDELCPCNWQKGEETLKAA"
     gene            14243..15142
                     /locus_tag="PKN91_01530"
     CDS             14243..15142
                     /locus_tag="PKN91_01530"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNS26779.1"
                     /translation="MNRLKLKDLTYLVALADQRHFGRAAEACNVSQPTLSAQLKKLEA
                     GLRVQLVERGSRRVALTAAGQRIVERARAMLEAGDDILEIARSFSDPLAGRLRLAVLP
                     TIGPYLLPRVTRRLRKALPRLELMLYEYQTAPMLERLRGGEIDAGILALPVDLDGLEA
                     RPLYDEPFVLLAPTGHRLARGGSVRTSDLAGETVLLLDDGHCLRDQALDVCSRVGMHE
                     KQDFRATSLETLRQMVAAGVGITLLPELATRGAYAAARGTVARPFARPAPVRTIGIVW
                     RRSSARRAAIQAVADIIGAHALA"
     gene            15150..18134
                     /locus_tag="PKN91_01535"
     CDS             15150..18134
                     /locus_tag="PKN91_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNS26780.1"
                     /translation="MTDTHPAATHVTPYDRLESHDVAIERLLVSGEQRETLIDYFGAA
                     AYVELSVLARRAAETARRRGPRVHILPGIMGSQLGRPRGHGLPHDILWLDPIDVAIGR
                     LREITLRPHSPVRAMGVILYNFLLLKLRLAVAGFAPRFFDYDWRRSVIDLGRAFAERL
                     DEDGSRDAHVVAHSMGGLVARAALALPGEARIGRVVMLGTPNSGSFAPVQALRGTYAV
                     VRKLATLDQVHSAETLASEVYSTFPGLYELLPAEGTTAIDLFDASAWPAAGPRPDADL
                     LAGARSALRGLADPDPRMTLIAGHARPTVTGVRRTARDFEYLVTRDGDGTVPLAHAEL
                     PGVRTYYVREGHSELTSNATVAAAIIDILRSGRTRRLSSQRPAPTRVAARIDDAALRR
                     THRRKIDWHALTHDERRNFLATLNDPPKLPLRATPARRDAKSAGAKHAGAKPARGALE
                     IVITRGDIQDADAAALAVGVYRGVRPAGAAAAIDLRLDGAIAELASRRMLSGDAGQIT
                     ALPAAGRLPHAQHAVLVGLGRFADLDAAAIERAAGSVARYCGLAHIASLATVPWGAGS
                     GLPTTLSFAAQLRGYLRHRAAPGHHLERIVFSVRDAAEHRAITQLAATLAREVDPDGR
                     QLFVRSVAARPAARAPRKAAALAPRLAHLLVERTAQGSRTDSWRAAVLTAGQHAAVIA
                     ETIDIARAQVGALLRELDTGRLTAARVAGIGARLAKLALHRNVRGALELTRNEPLVVV
                     NDAETSRLPWEMLALGEWSPAIGAGLSRRLSAPDLSVARFSAQRRADRTLTVLLVADP
                     TEDLPGAAREGARVRDIFRGLADARLTMITGKAATRARVLAEFESGDYDVVHYAGHAA
                     FDPRTPAASGLHVSDGVITGGDLDSLAQLPALVFFNACESARVRGAKTTGRATSLAAG
                     TSVAQAWLQAGIANFIGTYWPVADQAAETCAGTFYTALVGGDPIGAALLAARHAVRAQ
                     RSPDWADYLHYGDPLFALKTAP"
     gene            complement(18118..19092)
                     /locus_tag="PKN91_01540"
     CDS             complement(18118..19092)
                     /locus_tag="PKN91_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNS26781.1"
                     /translation="MNRRHLLGNAIVAAASAAAGVAAAGSARAFAADTVPGTDATATG
                     APATTGLITRRIPSSGEALPVIGLGTSGPMEVGTDAAARAPLRKVIDAFFEGGGRLID
                     TSPMYSSAERVLDDLLSARQQKQCFMATKVWADGAAAGIAQMEQSARLLKHEPLDLIQ
                     VHNLRDLDTQLATLRAWKAEGRVRYMGITHYTVAGQADLAEVIGREKLDFVQLNYSAV
                     TRDAEQRLLPLAADRGVAVIVNRAFEDGRIFAIVRGRPLPEWAAEFDATSWGQVFLKY
                     VLAHPAVNCVIPATGKVANQRDNLAAGRGRLPDASQRMRIVEALKAPS"
     gene            complement(19187..20509)
                     /locus_tag="PKN91_01545"
     CDS             complement(19187..20509)
                     /locus_tag="PKN91_01545"
                     /inference="COORDINATES: protein motif:HMM:NF026853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HNS26782.1"
                     /translation="MTIRTLEALFDAFRDLGATHTFCKRLAENDNSKQQIYLGGSLDA
                     LNMLPFGEVASDASAKRPNMKAPLKLLWLDEAGRTAPASGAQLILYPDYPEVRLSGFL
                     RGCARAPAAFLQPVPRERRKFDNAPDGRILFLANHPDRHLIAFLAPAGSAIAEEFENQ
                     AMRWRPRQRGVFLDIPLQGNDARSALLARLREIHAAGWHASRRLNAQRVAQPYIARNG
                     AGYTLEALFGIVPNSKAQPDFLGWELKACGSDRITLMTPEPDSGFYGKHGAEEFVRKF
                     GHQAGGDTLYFTGLHRVGELCRATGQTLAVRGFDIASGKIVDVGGGVYLIAPNGSESA
                     GWSFSRLLEHWGRKHAAAAYVPYEKEAAGARYRYLSPASLGEGTEFVRFLRALVDGAV
                     AFDPGSKVVDASTKKSKVKARSQFRIGKRRLRQLYERFTDEPLDPTNS"
     gene            complement(20506..20958)
                     /locus_tag="PKN91_01550"
     CDS             complement(20506..20958)
                     /locus_tag="PKN91_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HNS26783.1"
                     /translation="MVDAATRSRMMSGIRARNTRPERIIRSGLHRLGFRFRLHAAQPA
                     GRPDLVLPRYRAAIFVHGCFWHGHDCHLFRMPGTRRKFWSDKIRANRRRDAEVTRAVH
                     EAGWRQLVIWECAFKGLGRDASEKAVSRASAWIMGRRRYAEIRGSRLR"
     gene            complement(20960..22222)
                     /gene="dcm"
                     /locus_tag="PKN91_01555"
     CDS             complement(20960..22222)
                     /gene="dcm"
                     /locus_tag="PKN91_01555"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HNS26784.1"
                     /translation="MSEFYHLRQRAGLSIAMAAEAVGFSESTVYRWDRGDQVPRKAAL
                     EILRNLSVREVASPPSTEFRFIDLFAGIGGMRLGFESAGGRCVYTNEWNKYSQATYRA
                     NFPDDTHEIGGDIRTVAASAIPEHDVLVAGFPCQPFSIAGVSKKNALGRPHGFRCDAQ
                     GTLFFDVARIIEHHRPKAFLLENVKNLVSHDRGNTFRVIAKALTKELGYHIDARVIDA
                     RSWVPQHRERIFIVGFRDQTGFTFDDFVAPDPAKGPRLESVLHPEDGTEDIDDPRIGY
                     IAGPRGKVLPKYTLTEHLWRYLQNYAEKHRQKGNGFGFGLVGPQDVTRTLSARYYKDG
                     SEILVRQKGKPPRRLTPRECARLMGFDAAGRSPFRIPVSDTQAYKQFGNAVVVPVVRA
                     VAQHMKPWITGEASQDLFYSARKAANFG"
     gene            complement(22497..24281)
                     /locus_tag="PKN91_01560"
     CDS             complement(22497..24281)
                     /locus_tag="PKN91_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNS26785.1"
                     /translation="MYRAPLKDLQFVLNDLIGEGALASCPRHTDYSAELASSILEEAG
                     KLAEEVLDPLYRAGDQQGAKWTPDGVVTTPGFKAAYEAFTAGGWNQLSGDPEYGGQGM
                     PRMLGTAVQELWASANLAFKLCPMLTQGAIEALELCGSDAQKQRFLPKMVSGEWTGTM
                     NLTEPQAGSDLGQIRTRAAPDGDHYRLFGQKIFITWGDHDFTSNIIHMVLARIDGAPP
                     GTRGISLFIVPKFMVGDDGAIGARNDVQCISIEHKLGIHASPTCVLQYGARDGAVGYL
                     VGEANRGLEYMFIMMNAARLSVGLEGYAVAERACQQAAEWARTRVQGRPPVAATSGPA
                     SIIHHPDIKRMLLTIKSNTEAMRALAIYTAAQLDIAAAHSDEAARAAAQARGDLLIPI
                     VKAWSTEGGIRAASLGVQVHGGMGFIEETGAAQAYRDVRITTIYEGTTGIQANDLIGR
                     KIGRDRGAAMAALLADVARELEAIDSSDAAVQVAKRAALEGVAELSQATQALLAQLGS
                     AQDLALSVAVPYLELAGTVLGGWMHARSAAIAANKLAAGAADADFYRGKLATARFYAA
                     QVLPTADSLARIVQHGGASVAEVDAALI"
     gene            complement(24365..25276)
                     /locus_tag="PKN91_01565"
     CDS             complement(24365..25276)
                     /locus_tag="PKN91_01565"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNS26786.1"
                     /translation="MSTIWMPAQMALFARIVDLNGFSAAARALGVPKAAASRAIAELE
                     AALGVRLLERTTRRIHLTPVGRLLYPHCRRIVDEADAVRAAAASAIAVRGGPLRVMAD
                     PTYGRVLVAPIVPRFLERFPDVQLDVVMQAVDLENGTDWDVAVRVGAIDDARFVCREL
                     GAPPAVLCATPAYLQAHGTPAKPADLARHALLTPAATGPEYRVHLHRGTSQREEVSVH
                     PKLAVNDPAVLHASTAAGLGIGLLPEFLCRQGLVTGKLVRVLPDWSLPATMPLCAIYP
                     ATLATDRRVQVYVEFLAANVIPALATG"
     gene            25332..26327
                     /locus_tag="PKN91_01570"
     CDS             25332..26327
                     /locus_tag="PKN91_01570"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNS26787.1"
                     /translation="MRLRKLGTQGLEVSAIGLGAMGMAGVAGMADMYGAVDEAEAIAT
                     LQRAIDIGVNFIDTAEVYGPYTNESLVGRALSGRRDRAIIATKFGFRLDGGNRLGVDG
                     SPANVRRAIDGSLERLGVDHIDLWYQHRRDRDVPIEDTVGAMAEQVRAGKVRYLGLSE
                     VGAETLRRAHAVHPISALQSEYSLWERNIEDEVIGVCRALGIGIVPYSPLGRGFLAGK
                     LPDPATLPSGDYRRRFDPRFEPDNYARNLAIVREVERIATGHGATAAQVALAWLLHQG
                     DDIVPIPGTKRRKYLEENAAAADLALEAADLAALKALQPAAGPRYTETAMASIER"
     gene            26461..28020
                     /locus_tag="PKN91_01575"
     CDS             26461..28020
                     /locus_tag="PKN91_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNS26788.1"
                     /translation="MFKLRRAKPAARAKVERPLAERERVTPEVLGRELREALRQIRVH
                     SISIHNDECDALWLSEGVLGPDEHSVALEAMGAFGLETSRQYADFDLGDGRGAVCYPA
                     RSPYGEVLGLAMVIADTKSIENGNGERLATPEVRAILQRLTVVLRPPSATQTQPIARP
                     DALAIAAAGTASAPAATVALSPVVTVRPAAVVAPPAREATPPAPAPAPAAAQVPVVDP
                     AASDCDMPVIEFSFNDLAEDLADEPVVAPAATPAPAARPADTSADATLYVPQATVREV
                     ALHVQQLHKLRSGGRTRRFEVLLRARSHPDADAMTDTVAEMLASQGTTSTMDRYITGE
                     LLTWLASHTEVWEREPASFSVNVTLSTLTDEQFLPYVSSRLAEARVAPEVLGFEVPER
                     AFIEHRALAERFVRVCEKLGCFVVLDDFTLHSEALPFLASPAMRLVKIDARLTSAALK
                     EKLPQAIVIAISQAAKVLGVHCVAKRVESQVGRQWLSAIGIDFAQGFLLEKPQPLDDL
                     ALQAVKAASEG"
     gene            28135..29238
                     /locus_tag="PKN91_01580"
     CDS             28135..29238
                     /locus_tag="PKN91_01580"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNS26789.1"
                     /translation="MPTAAAPEAATRLLGGLRISRNTLFWCLHAAGWFAYGCVQYLGS
                     LIYEKETGYQLVILSAAIAGFVVSMPLRYIYRMLWRQSPRAILLGILATSYLTALVWR
                     LCVNFAYERFMPEMDWEITHWYQYFGGANQGTYLMLCWSAFYFGIKYYEMLQLQREAT
                     LRSAALAQEAQLRMLRYQLNPHFLFNTLNAISTLILDNQNRTANQAVTRLSDFLRYTL
                     DQDPMKKVTVRQELDALNMYLSTEQLRFNDRLRIEFAFEEPALEALVPSLLLQPLIEN
                     AVKYAVSPREGGGTIRVEGRVRGAMLELTVADDGPGARTSSALIEGRGVGLRNTRERL
                     HVLYGDTHRFAAINAHPGVRIEIALPLEKASIP"
     gene            29235..30071
                     /locus_tag="PKN91_01585"
     CDS             29235..30071
                     /locus_tag="PKN91_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNS26790.1"
                     /translation="MTNSQIRTLIVDDEALSRRGLEIRLRTAGDFDVIGQCTNGREAI
                     DAVRNQQPDLVFLDIQMPGLSGFDVLAQLPTDSLPMVVFVTAYDQYAIRAFEARAVDY
                     LLKPVDESRFAAAIEHIRELRQQRNAASQRDRLVELLADVSGTGEIDAEGLLARGAAA
                     HAAERNTVLPIREGRETVRLDTKSIDWIDAAGDYMCIHAGPRTHILRGTMKELEDVLD
                     PRHFQRVHRSTIVNLARVKTLRSHMNGEYFLTLDGGHELKLSRTYRDKVEYFLKGPPR
                     LN"
     gene            complement(30046..32166)
                     /locus_tag="PKN91_01590"
     CDS             complement(30046..32166)
                     /locus_tag="PKN91_01590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNS26791.1"
                     /translation="MEPVHGALYRRRGSVLQSGAPPRHAGRRLLVVVAAIAALPCASA
                     AARGIVAEDVYKLQELASPSLSPDGEWIAYTVSTTDRDADADQTDIWLARYDGSQVIQ
                     VTRSTSSEHSPRWSPDGRRLAFLSDRVDEKAGDQIWVLEMAGGEAQQRSRFETAITDF
                     AWSPDGRRIVFSARLPRYEDGDEDLPAPIVIDRLWFKSDAAGYLREERSHLYLLDVES
                     GATQQLTDGAFDDVQPAWSPDGTQIVFVSKRGEAPDAHGNWDLYVVAPQPGAGARQLT
                     THPGTDGDPEEDWLSGPPRYSPDGRRIAYLAGGAPADIWYGLLQAAVIEVPAGAREAP
                     AAARLPSAVLDRNTLEPRWSADGRWLYFRLEDDMSMVLARVRLADGRIERLTAPGSVV
                     SEYDVGVRGRVVMVHGTSAQPGELVALERGRLRRISHHNDAWLAGVDLVTARTTTAQS
                     ADGLEIHALVYEPRGRPAGTRHPTLLRLHGGPVSQFQHEFDLQLQLFAAHGYAVLAPN
                     PRGSSGRGYAYQRALFANWGAADVPDVLAITDRALADGLADPARLGVGGWSYGSILTN
                     YVIAADTRFKAATSGAGMSNMLGGFGLDQYLREWEAELGLPWQNTDLWLRLSYPFLHA
                     DRIRTPTLFLCGEDDFNVPLAATEQMYQALRRVGVPTRLVIYPGQKHGLGRPSLRVDR
                     LQRYLDWYATYLPPRPLSSAAAVP"
     gene            complement(32076..33269)
                     /locus_tag="PKN91_01595"
     CDS             complement(32076..33269)
                     /locus_tag="PKN91_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HNS26792.1"
                     /translation="MTIPREHYRQAVFPTPFHTRTAAANTLNVWHRWRDYTVADAYFD
                     VSLEYTALRNACTVFDLSPMTKHLITGPDALAFMNRLVTRDVAKLKPGRVGYTVWCDD
                     AGQVVDDGTIFHLRDGVYRLCSQERQLDWLHASALGFDAAIQEETHDVAALALQGPTS
                     CAVLKRLGLAGIETLTPFGIRSFAFEGGELTVSRTGFTGDLGYELWIDPELGIALWDR
                     LFDVGAPVGIKPMGTHALEMSRIEAGFIQAGVDFLPADRAVRAGRTRSPFELDLAWLV
                     DFGKPHFTGRRALLAEQARGSRHRLVRLDVEGNKPAKDAYLYDRRRRNVGVVTSAMWS
                     PAAKANIALASVEMPHGAPGGELWAEIYYQKELKWSRFMARCTVVEGPFYSPARRRAT
                     PAADF"
     gene            complement(33266..>34759)
                     /locus_tag="PKN91_01600"
     CDS             complement(33266..>34759)
                     /locus_tag="PKN91_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNS26793.1"
                     /translation="AGRKVLVLEAADQVGGAAVTREFAPGFRVSSVAHLLHALDPRIE
                     RELRLAQHGLAYARRELLTVALGESAPLVLDGANVLAGDVSSADRAGLVDYVRRQRRF
                     AALLAKQHGFGAGFSVLRLFGRSAKNRNTESPLPWLARFALDVRLLGRADMRELLRVG
                     TMNLYDLLEDRFDSAQLKGALALDGVLGARLGPRSGQTFFTSLQRAGARGLPRGGMGA
                     VTGALAVAARAAGAEIRVASPVASFMLDGERVTGVVLQSGEQIAAATVVSNVDPQTTF
                     LGLLGARHLDAEFARRVAHIRSTGCAAKLHLALSGPPDFRGVPAELLGERLVIAPDLG
                     YIEAAFNPAKYREYSSQPVLEITVPSMHDPGLAPPGQHVLSAIVQYAPRDLAVGWAAG
                     RAPFLEATLGVLARHAPALRSQIVAAELLTPLDIENDYRIHGGHWHHGELALDQYMML
                     RPVPGAAYYRAPVPGLYLCGAGSHPGGGVIGAAGRNAARVVLARSRA"
BASE COUNT         5619 a        12175 c        11640 g         5325 t
ORIGIN      
        1 ccacccccga gacccttcgc ggcccggcgc acgagtggtg cgatgatgcc gtgcccgatg
       61 cgggccgcat atccccgctg cggcagcgca gcctgcagcg cgcggagcgc cttatagcgt
      121 gctcgcgccg gcacgagctg ccacagcacc gaacacgcgg cgattgcgac tatgacgaag
      181 gcgagcgcgt tcatgtggtg agtgcgagcg cgacccgata ggtgatgaac gaagcgagat
      241 aggcgagcgc gaacaggtaa cccgccatga tcagcggata gcgccagctg ttcgtctcgc
      301 gcttgacgac ggccagtgtc ggcaggcact gcggcgcgaa cacataccag gcgagcagcg
      361 acaggccggt cgcgaggctc cagcccgagg cgatcaccgg catgatcgcg gtttcgagcg
      421 ccccttcggt ggccgacatc gcgtagacgg tgccgagagc gccgatcgcc acctcgcgtg
      481 ctgcgaggcc cggcaccagg gcgatcgaaa tctgccagtt gaagccgatc ggcgcgaaaa
      541 tcgtgtgcag cgcgccgccg agcatgccgg cgaagctgta ctcgatcgcc gggcccgttg
      601 catcgggcgg cggcgcaggg tagctcgaca gaaaccacag gatgatcatc agcgagagga
      661 tcacgccgcc cactcgtcgc aggaagatgc gcgcgcgatc ccacaggccg accagcaggc
      721 tgcgcgggtc cggccactgg tagtcgggca gctcgagcat cagcgggtga tggttctgct
      781 tgccggcaaa gtgcttcatg aaccacgcga cgccgagcgc actcagaacg cctgcggcgt
      841 acagcgcgaa cagcaccagc ccctgcagat tgaagggacc ggccgccgca gccgggatga
      901 acgcgccgat cagcagcgca tagaccggca ggcgcgccga gcaggtcatc agcggcgcga
      961 tcatgatcgt gaccagccga tcgcgcacgc tcggtatggt gcgcgcggcc atgatgccgg
     1021 ggattgcgca ggcgaagctc gacagcagcg ggatgaacgc gcggcccgag agccccacgc
     1081 tgcccatcag cttgtcgagc aggtaggccg cgcgcggcag gtagccgctg tcctcgagcg
     1141 cgagaatgaa gaaaaacagg atcaggatct gcggcaggaa caccagcacg ctgccgacac
     1201 cggcgatgat gccgtcgacc agcaggctgc gcagcgcgtt gtcgggcagc gcgcccgaga
     1261 cccaggtacc gatcgccccg acgccggcct cgatgagacc catcggcacg gcggcccagc
     1321 tgaacacggc ctgaaacacg aagaagagca ctgtaccgag aaccagcatg ccgccgaacg
     1381 gatgcatcag gatcgcatcg gcgcgcgctg cggcgcgcgc gcgcagcggc tcgccgtagc
     1441 ccacctccgc gagcagccgc ggcaccagct gctgcgtgcg ttccacctcg gcgcgatccg
     1501 gcgcggcgat ggcgtgggcc gcgccatcga ccgccgggac ggcgtcgtgg agcacggcag
     1561 ccgcctcaaa cgtcccgagc gcctcgatca gctgatcggt accgccaggt gcgatcgcca
     1621 cggtttctac gatcggcacg ccgagcttgt cggcaagctt cgccacatcg agcgtgaagc
     1681 cgcgccgctt cgcgatgtca ctgcgattca gcgcgacgat catcgggcgg ccgagccgct
     1741 gcaggtcgag gatgagccgc agcgacagcg gcatatgggt cgcatcggcg acgcacacga
     1801 tcacgtcggg ctgcggctcg cccgcgatgc ggccgagcac gacgtcgcgc gtgatcgcct
     1861 catcgagcga ggccggcgtg aaactgtagg tgcccggcag gtcgaccacg gcgaagcggc
     1921 gcccggcacg gttgtggaac tggccttcct tgcgctcgat cgtgacgccc gggtaattgg
     1981 cgaccttctg gtggctgccg gtcaggcggt tgaacagcgc cgtcttgccg cagttcggca
     2041 cgcccaccag cgcaatgcgc agatccgcca gcgcgcgatt ggcggcgtcc atgtcagcct
     2101 gcgccgttgg ccggcacaac cattactgcg gcggcctcgc cgcgccgcaa tgcgaaagtc
     2161 gatccgcgca cccgcacggc gagcggatca ccgaacggcc acatcgactc gacgacctcg
     2221 acttccgcgc cctgcacgaa gccgagttcg acgagccgct ggccgacggt ggaatgcagc
     2281 gcatcgccga cggacagcgt gtcatcgagt acgcgatgca cggtcgcccg cgcaccgcgc
     2341 atcagcgtgg acatgggtac cggcccgccg ggagattcag tcatacccgg cagtatcctg
     2401 cgagcgcgaa tggttcgcaa ttgcggccgg caagcacttc catctagcca tattgctgca
     2461 tgaaagattc aatagcctac cattcgtacc gtatcgttgc gcccgctgtc cgacccgggc
     2521 gcgtataacg atccaaccag cggttgctgg ccaagcgacc ggagacgtcc gcccattccc
     2581 agtatttctc gtcggtcaga ttgaacaacc cggcgttgag gcgcaattgc ggcgtgatgg
     2641 cgatgtccgc cagcaggtca actttcgtat aggagggcgg gacgaacggc tcgccgaggc
     2701 tgtcgtcgac acgccgcgga ctgtcggcga aggtcgtgac gagctcggcg ccccagcgct
     2761 gcgacggtgc ccgccaggcg aagccgatca cggccttcgg cggatcgatc gagttgaggg
     2821 gcgtgtcgtc ggtacggttc tcaccgcgcg catacgacgc cgcgagccgc accgacagtc
     2881 cgcgcagcga gtcggactga ccctccagcg gcatgccggc ggccacttcg acgccgcgga
     2941 tgcgcacctc attgaggttc tgcgactgga acacgatcag gccgcgcgcc ggatcgaggc
     3001 tgccgaccag cgactcgatg aaatcgtcgt acaggttgta gtacgcggag agcgcgaggt
     3061 agtggccgtc gggacgtgcg aggcgccagc ccagctcgaa gctgtcgctg gtctcggact
     3121 tcagatccgg attcgcgatg gcggtgtagc cgaacgcgag gttctcgaag ccgaagttcg
     3181 catcgtcgta gggcggggtg cgaaagcccg ccgcatagtt gaagtagccc gtcgagtact
     3241 ccgtgatgcg gtacatcacg ccgagcttgg gcgagacgtt cgtttcgtcg atgtccgcgg
     3301 ccacgaagcc cgggttgtcc gccgcgaaga tcgcgtcgac cgtcgggcgc agtgcgtaat
     3361 cgtcgtagcg cacgccggga gtgatcgtcc aggcacccag gctgatctcg tcctgcacga
     3421 agatggacgc gcggcgggtc ttcgtcagcg gaaaatcgcg caccgggaag acgtccggca
     3481 ggatcgccgg gctcgtgacg ccggtcagca ggttggtctg caggcccgtc ctcagctgca
     3541 cggtgtcggt ctcgatgtat tcggcgccgt aggcgagcgt gtgcgcggcg ccgagcgcct
     3601 cgaaacggct gaacagtgtc acttcggccc cgacttcctc ctgttcgaag tcgaacaggc
     3661 gccagcgcgt ctgcggcgtg atcacgcccg caagggtcgc ggcgttgcgg ctctcgagtg
     3721 tctcctgcga ggtctcgctc tcctgccggt atacgcgcca ctcaccgagc gggaaggcat
     3781 ggtcgaaagt gatgcgcgaa cgctgctgct cgtcgctgcc gacgagcccc gtggtctgtg
     3841 tgccgcccgc caccggcggc gtcgccgata ccgcggtcag cacgtcagta aaggtatcga
     3901 tgcggctgcc ctcccaggtg aggcgcaggc gctgcgtgtc ggtcgcatcg aagaccagct
     3961 tggcgagcac gctgtcgctg tagcgatcct gcggcaccgg ctcggtgcgc atcgtgccga
     4021 ctccgccgat atcgccggag ttttccgttt cgtggccatc gcggcgcgtg taggcgagca
     4081 gtcccgagaa ggcccccgcg cgcgccgcga acgtcccgcc ggcgacggtc gagtcgttcc
     4141 ggcccgagta gccggccttg gtcgagaagc cgtagttcct gtcggcagac agcaggtccg
     4201 ccggatcctt ggtcgtgaag gcgaccacgc cgccgatcgc gtcggagccg tagagcgacg
     4261 acaccgagcc gcgcacgatc tcgacgcgct tcagcagatc ggtatcgacg acgtcgcggc
     4321 ccgcgtcggc ataactgccg atcgagaacg cgtccggcat gcgtacgccg tcgacctcca
     4381 tcagcacgcg attgccgccg atgccgcgga tggtgatgtc ggacaggccg aatcgcccgc
     4441 cgtcatcgct caccgtcacc ccgggctcgt agcgcacgag gtccttcagg tcgaacacca
     4501 attcgcgatc catgtcctcg cgacccttga cggtgaccgt cgccggcaca tcgacgacgc
     4561 gggtctcgat gcgggtcgcg gtgatcgtga cgacctcgag gtcgacggtc tgagccgcga
     4621 gcggcgctgc ggcaaccgcg agcaacagag aggcgggccg cgcgaacgta ggagtgctca
     4681 tcaatcttgc tttcgttgga gatgggggac gcgaataata acgattcgca tttgccgcac
     4741 aatagggatt gtgcaccgtg gacaagcatg taatttttga cactctgtca acagagagct
     4801 agccttgcgc catgagccgc attgccgaac gccgccagga ggagaaggaa cgccgccact
     4861 cggacatcat cgacgcggcc gaggcggtcg ccgcgaccgt cggcctcgac gccatgacga
     4921 tggacgaagt ggcgcgcaag gcgcgcctgt cgcgcgccct gctctacgtc tatttcaagg
     4981 acaagccgga cttgctgttc gcgatctgcc agcgcgcgct cgagaacctg cgggtccgtt
     5041 tcgaggcagt ggcacacagc cacgcaacgg gactcgccgc gatccgtgcc atgggcacgt
     5101 ccttcgtcga gtactcccgc gagcagcccg tgcgttacga cgcgctcgcg cgcttcgcgc
     5161 gccacgcccc gcagcaggcc gacggtgccg cgaacgagcg cgcctgcctc gaaaccggcg
     5221 acagtgtcca tcgcatcatg gacgcctgca tcgaacgcgg catcgccgac ggctcgatac
     5281 gcgcggacgt cggcccgccc ggcctcgtcg cgctttcgct gtggagttac acgctcggca
     5341 tcatccagct cgccaccacc aaggcggcgg tcatcgagca ccgcggctac gacgtcgagc
     5401 acctgatcga gcacgccttg cagatggccg cgcgggcgct cgcgccgcgc tagccgggga
     5461 ggaagtcgtc atgccagcca gagccgcaac atctgtacgc cgcaggctcc gcgccgccgg
     5521 atgcgccctg tgcctgctcg cactcgcgct gcccttcgcg ggcgcggtgg ccgcgcgcag
     5581 cctcaccgag gtcgtcgatg agtacgttcg cgagggcctg cgcgccaatc tcgcgctgcg
     5641 cagccaggcc ttcgacgtcg agcgcgcgat cgccgccctc gatggcgcgc gcgccggcta
     5701 cctccccacg ctcggcctcg atgcgcgcta cacgcgcagc gagggcgggc gcgagttcca
     5761 ggtgcccctc tcccagctcg acccaatgct caccgaccag acgattccgt tcctgcgcga
     5821 gcgcgagcag gacacgcgcc tgacgctgct gcagccgctg tatgcgccgg cgatcccggc
     5881 gagcgtgcgc gcgcagcgct cgcggctcgc agcgagcgag tacgcacagc tcgcgctggc
     5941 gcggcgcctg cagcgcgaca tcacggtcgg ctacctcgac tggttgcggg cgacgaagac
     6001 cgtggcgatc gtcgaggcga gtcgcgtgct gctcgacgag aacctgcggg tcaacgattc
     6061 gctgttccgc aatggcaagg tgacgcgcga ccaggtgctg cgcgcccacg cggaactgct
     6121 cgaggtgcag cagcagctgc gcgaggcgca gaacctcgcg cagcaggcac gcagctacct
     6181 gaatttcctg ctgaaccgcc cgctcgacac ggcgctcgaa gccaccgaag tcgacgaggt
     6241 cgtggcgcgc tcggccgcgg acttcgcgac cctggcggcg gccgcgctcg aacgtcgccc
     6301 ggaactcgcg caggccgaaa gcgccgtgca ggccgccgag gcgcagatcg gcgtcgaacg
     6361 cgcacaactg aaacccacgc tcggcatcgg cgtcgacggc ggcatccagg gtgaggacta
     6421 cggtaccggc cacggctaca actacggcca ggcgtcgctg cgcctcacct ggaagctgtt
     6481 cgacggcggt gcgaaccgtg ccgccgtgcg cagcgcccgt gcctacgcgc gccgtacggc
     6541 gaccgagcgt gacgagctgg cacagcagat tcagctcgag gtgcagcagg cgctcgaccg
     6601 gctgcatacc gccggggatt cgctcgccac cgccgaagcg cgctcggagg cggcgcgcgc
     6661 gggctttcgc atcgcaagcc gcaagcgcga cgaaggcgtg atcagccagg tcgagttcat
     6721 cgacgcacgc agcacgctga cgggcgccga attgaacctc aatctcaccc gcttcgcgct
     6781 gctcgcgcgc caggccgaac tcgattacgt gactgcggcg ggattgctgc cggtcgaccc
     6841 ggcatccaca ggctccgccc catgacgcga caggtccgac ccggctggtt gctgctgctc
     6901 gccctcgcgc tcgcggcgtg cagcgacagc gcgccgtccc cgcagccccc ggccacgccg
     6961 gtgcgcgtgg ccgtcgcgca tgccggcccc gccgtcgcgc ccttcgcgac caacgggatc
     7021 gtggcggcca aggacgaaat gcgcctgtcg ttcaagatcg gcggcatcgt gcgacgcatc
     7081 gccgtcaccg agggtgagcg catcgcgcgc ggtcagctgc tcgcggagct cgagctcacc
     7141 gaaatcgacg cgcagctcga gcaggtcgcg cagctcgccg ccaaggcgca gcgcgacctc
     7201 gcgcgcggag agcggctgta tgccgaccag gtgatcacgc tcgagcagct gcaggacctg
     7261 cgcacgcagg cctcgatcgc gcaggcgcag gccaccggcg cgcgcttcga ccgcggacac
     7321 gcggtcatca ccgccccgca tgacggcgtc gtgctgagga agtacgtgca ggagcgcgag
     7381 atcgtgggcg ccggcgcgcc ggtgctgatg gtgagcggcg ccgagcgcgg ccacgtggtg
     7441 cgcgctgcgc tcgccgatcg cgaaatcgtg cagttgcgcc tcggtgacac cgccgatatc
     7501 cggctcgacg cgtatcccgg gcgacgattc agcggcacgg tcaccgagat ttcgggcgct
     7561 gccgagacgg gcagcggtct cttcccgatc gaggtgcgct tcggggagga cgcgccgcag
     7621 ctcgcgagcg ggctcgtcgc caagctgacg atccaccccg ccgcgcccgg cgcgacgaat
     7681 ctgacctatg tgccgatcgc ggcgatcgtc gagggcgacg gcgagcgcgc cagcgtctac
     7741 gtgctgcgcg aggggcgcgc cgagcgccgc gcggtcaccg tggcgttcat cacccacgac
     7801 ggcgtcgcac tgagcgccgg cgtcgcgagc ggcgagaccg tggtgacgga cggcgcgctc
     7861 tatctggccg acggcgagca gatccgcgtc gtcggcgatg ccgcggagac catcggcgcc
     7921 atcgcgctgc cggggcgcgc ggccgagtcg tgaccgcgct cgaattcccg atccgacgct
     7981 accagttcac gctggtcgcc ttcctgtgcc tgatcgcgct cggctggtat gccttcgcca
     8041 atgtgccgcg cgaggaggac ccctacttca agatcccggg cttcttcgtc accgcgatct
     8101 acccgggcgc cgatccgcgc gacctcgagc ggctggtcgc caagccgatc gaggaccggc
     8161 tcgccgagct cgacgacgtg aagaagatcg agacgacgat caccgacggc gtgtcgttca
     8221 ccgccatcga gttccaggcc agggtggatc ccgacgaagc ctacgacgag gtgatgcgcg
     8281 aagtgaacgc gctgcgtccg acgctgccgc aggagatcgc ggagatcgag atcaggaagc
     8341 tgagccccgg gctcgtcaac acggtgcagc ttgcgctggt gtccgaggag gcgacctggc
     8401 gcgaactcga ggattacgcg cgcgacctga aagatacgct gaaaccggtg accggcatcc
     8461 gcacggcgga gagctgggcg atcccgcggc gcgaactgcg cgtgcaactc gacctgaagc
     8521 gcatggccga gatcggcgtc acgccggcgc aggtcgcgca ggcgatcgcg agcgagaacg
     8581 ccaacatccc ggccggcacg atcgacatcg gcccccgcgg attcagcctc aagacctcgg
     8641 gcgcctacga gaccctcgat gaagtgcgcg acacggtggt cacggcgggc ggcgggcacg
     8701 ccgtgcgcat ccgcgacatt gccgcggtga gctggactac cgcgcccttc agccatgtcg
     8761 ggcgctacga cggccggcgc gccgtgttcg tcacggccaa ccagaaggag gggtacaaca
     8821 tcctcgaggt gcagcagggc atcgacgccg ccctcgaccg cttcgaggcg ggcctgcccc
     8881 agcgcatcac cctcgagcgc ggcttccagc aatccaagaa cgtcgagcag cgcctcgggc
     8941 gcctgtacac cgacttcgcg atcgcaatcg cgctcgtgat gctgacgctg gtgccgctcg
     9001 gctggcgcgc ggcgggtatc gtcatggtgg cgataccgct gtcgctcgcg ttcggcatcg
     9061 cgatcctcta cctgatcggc tactcgctga accagatttc gatcgccggc tgtgtcgtcg
     9121 ccctcggact gctggtcgac gattcgatcg tggtggtcga gaacatcgcg cgccatctgc
     9181 gcatgggact cgatcgcact gccgcggcgc tcgcgggcac ccggcagatc ttcgtcgcga
     9241 tcctcggctg caccgcgacg ctcatcttcg ccttcctgcc gctggtcatg ctgcccggca
     9301 cggccggcaa gttcatccgc gtgctgccgg tgaccgtcat cgcaacgatc accggctcgc
     9361 tcatcgtggc gctgttcgtg atcccgttcc tcgcgagccg cctgctgccg cgcgatgccg
     9421 cgcaaagcgg caacccggcg ctccagcgcg tgatgggggc gatccatcgc tactaccgcc
     9481 cggcactgca ctactgcctc gccaggccgc gcgccaccgt actggtctcc atcggcggat
     9541 cgctcctgct gtcggccgcg ctggtgccgc tgatcggctc gagcctgttt ccgaaggcgg
     9601 acacgccgca gttcctgatc caggtcgagg cgcccgcggg cgcgagcctc gaggcgaccg
     9661 acgaggccct gcgcttcatc gaagcgaagc tacgatcgac gcctgcggtc aagggctggt
     9721 tcaccaacgt cggccacggc aacccgcaga tctactacaa ccacatcacg cggcgcgacg
     9781 cggcgaattt cggcgaagtg ttcgtgcagc tgacgcacta cgacacgcat gagacgccgc
     9841 gtacgctcga ggcgctgcgc actgcgctgc gcgactatcc ggcggcgcgc atctacgtga
     9901 aggagttcgt caacggtccg ccgatcagcg caccgatcgc ggtgcgcgtc gtcggccgcg
     9961 acctcgaggc gatcgaggcg ctcgccggcc aggtgcagcg gctgatcgag gcgacgcccg
    10021 gcacgcgcga cgtgcagaac ccgctcgagg tggcgcgcac caacctgcgc ctcgccgtcg
    10081 acgcgcagaa ggcgaacctg ctcggcgtgc cgaccatcga gttcgaccgg gcggtgcgcc
    10141 tgtcggtgga gggcctgccg gccggcaagt tcaaggatcc ggacggcgag cagtacgaca
    10201 tcaccgtgcg caccgcggtt ggtacgcgcg cggacctcgc gagcctcggc gaagtgcgcg
    10261 tgccgtcact gtccggcgcg ctgctgccgc tgtcgcagct cgcgacgctg cagttcgaga
    10321 aggcgccggt cgtgatccag cgctatgacc gcgagcgctc ggtgacgatc gatgccgacg
    10381 tgcaggccgg ctacaacacc gcgaaagtca cggccgacgt gcgccgccag ctcgatgcga
    10441 tggactggcc gcgcggctat cactacaagc tcggcggcga ggccgagtcg agccaggagg
    10501 ccttcggcgg catcggcatc gcggtcatcg tcgcgatctt cggcattttc gcgatcctgg
    10561 tactcgaatt cggcaacttc aagtcgaccc tgatcgtcct gaccgtcgtg ccgctcggcg
    10621 tgctcggcgg gctgctgatg ctgctcgcga ccggcaattc catctctttc accgcgagca
    10681 tcggcttcat cgcgctgatc ggcatcgaga tcaagaattc gattctgctg gtcgatttca
    10741 ccaaccagct gcgtgcggat ggcgtagcgc tcgacgaggc gatcgagcag gccggcgaga
    10801 tccgcttcct gccgatcctg ctgacgacgc tgacagcgat cggcggcctg ctgccgctcg
    10861 ccgtgcagaa catcggcctc tactcgccga tggcgtgggt gatcatcggc ggcctgatat
    10921 cgtccacgct gctcgcgcgg ctcgtgacac ccgtgatgta caagctcgcc ccgccggcga
    10981 tcgccccggc gggtcagccc atcaggccga gcacgagacc catgaccgtg taggccacgg
    11041 tgtggtgccc gccgttgatc aggaagtggc gcaggctgcg gcgctcgaac aggtagtcga
    11101 cgcccttgcc gaacgcgacc cagaagaggc ccgcggcaaa tccggcgccg gcgccaaact
    11161 gcacgcccgg gctcgggccg aggaacatgt ggaacacgag cgtcgacagc agggtcagca
    11221 cgaaggccga accgaggatc ctgcccatgt tgcctttggc gaggtcggtg tccgatatgc
    11281 cgatctcgcg ctgccacgcc ttcgcgaaca gcagcgggga ataccacacc gcgccgacca
    11341 ggaatgacgc gatcgccgcg acggcgacgg ccagccagtt gatttccatc agcgtacccc
    11401 tcgttgaacg gttgtcatgc gctgaagagc caaaattcac gctacgcccg gaccccgggg
    11461 ctgtcaacgc aggtagcatt gcgccgactt gcccgcccgg agattctctt gcgatccgtg
    11521 ttcctcgact acgccaccgt ctcggcgaac gatctcgacg cctcgggact cgcgcgcgcc
    11581 tgcccgaacc ttgagtttta cgatgtcacg cccccggggg aggtcgccgg ccgcatcgcc
    11641 ggtgctgcga tcctgctggt caacaaggcg cgcatcacgc gcgagctgat cgaggcgacg
    11701 cccaccctgc gcctgatcgc gctcgctgcg accggcaccg acaatgtcga cctcgccgcg
    11761 gcccgcgagc acgacgtcgc cgtgtgcaac atccgcgact actgcacgcc ctcggtcgtg
    11821 cagcacgtgc tgggcgcgat cctcgcactg acccatcgct atgccgatta ctcgcgcatc
    11881 gtgaccgacg gccactgggg ggagagcccg cagttcacga tgctcgactt cccgatccgc
    11941 gagctcggcg ggcgcacgct cggcgtcgtc ggctacggca cgctcggtcg cgcggtcgcg
    12001 cgtgcctgcg agcaggcgct cggcatgcgg gtgctcgtcg cgcgccgcga aggcggcccc
    12061 ggccgcgtcg gtgaggcgga cacggacgcc cgcgtgccgc tcgacgccct gcttcgcgtg
    12121 gccgatgtgc tgacgctgca ctgtcctctg acgccggcga cgcgcggcat gatcggcgcg
    12181 gcgcagctgg ccatgatgaa gcccgacgcc gtgatcgtga acaccgcgcg cggcgcgctg
    12241 atcgacgtgc aggcgctcgc cgatgcattg cgtgcgggca ggctcggcgg cgcggccatc
    12301 gacgtgttgc ctgaagagcc cccggtgtcc ggcagtccgc tgttcgcgcc cgacctgccg
    12361 aacctgatcg tcacgccgca cacggcctgg gcggcgcgcg agtcgcgcca gcgggcactc
    12421 gaccggatgg cggagaacgt ggcggatttc ctggccggcg gacagctcgg ccgcgtcgtg
    12481 taacggggtc gcagcgcggg gtcgccggcg cggcctcagt cgtgcgtgcc gaggcccagt
    12541 acgatgccgc gcgcccactc gagcacgggc gcgacgcgcg gcgaaacgag gtcgagcgct
    12601 tcgtcgaaag tcacccagcg ccattcgtgg tgttcgggca tgcccaggtc ggcattcacg
    12661 ggcagcgtga cgcgcgcagt gtgcgtctgc gcgatgtagt agcgcgccac cttattgcgg
    12721 gtgtagggac cggtgtcgcg gtagaggtca ccccaggtga actcgaggtc gtcgatcgcg
    12781 gattcctcgc gcacttcgcg gcgcgcggcg gcgtgcggct cctcgccggc ctcgaccatg
    12841 cccttgggaa aatcccagct tcgatatgcg cgcagcagca ggaacagcca gctgtccccg
    12901 tcgcggcgca cgacgaccac acctgccgac aggcgtggcg cccggttcgc gttcatcgtt
    12961 ggcggagcat atcagccctg ggccgattcg agcactccgg caacggcgtg gatcacggcg
    13021 gcgatccgca ccgagctctg gatcgtctcg cgcgtcacgc catgctcgcg cagcttcttt
    13081 tcgtgggact ggatgcacag gccgcagcca ttgaccgcgg agatggcgag cgacagcagc
    13141 tcgaaatcga gcttgtcgat gccggggtta ccgatgacgt tcatgcgcag gcgcgccggc
    13201 atggactggt actcgccgtc ctccacgagg tgcaggaacc ggtagtagat gttgttcatg
    13261 cccatgatcg cggcagccgc gcgcgcgcca ttgacgtcgg catcgtcgag atgctcgcgg
    13321 gcgagcgcct cgatgtcgcg cgcaaaggcg gggttgcgcg cagcgaccgc cgtcgccagc
    13381 gcgaccgacc acaactgctt gacgctgagc cctggcgccc cctgcgtcgt cagtaccgaa
    13441 ccgaggttca gcttcaggtc gcgggcgtag tcggggatgg cgtcacgaat ggtgtcgagg
    13501 ctcatggtgc gtctccgggt gcagcgaatc cgtcaacagg gaggttgggc gggggcgaaa
    13561 gacccggggg gctggtcttc cgcgtccgcc ctcactcatc aggcagcctt cagggtttcc
    13621 tcgcccttct gccagttgca ggggcacagt tcatcggtct gcagcgcgtc gagcacgcgc
    13681 agcacttcgt ccgggctgcg gccgacgtcg aggtcggtca cgtagacgaa gcggatcacg
    13741 ccctccggat cgacgatgta ggtcgcgcgc tgcgccacgc cgacagccgg gtcgatgatg
    13801 ccgagctcgc ccgacagctc acgcttgagg tcggagagca tcgggaacgg caggtccttc
    13861 agctcttcct tttccctgcg ccaggcgagg tggacgaact cgttgtcgat cgagacgccg
    13921 agcaactgcg cgtcgcgcgc cttgaattcc ttgtcgagct tgccgaaggc ggcaatctcg
    13981 gtcgggcaca cgaaagtgaa gtccttcggc cagaagaaca ccacctgcca cttgcccgtg
    14041 aaactgtcgt tcgtgaacgg cttgaacgcg gtcgcgacgt cgttcgagac gacgccggtc
    14101 agcgaatact gcggaaactt ctgcccgata cccaacatgg tctgctgctc cgtttattgc
    14161 caaaggacgg cgccattctt ggtgacgccg agccatacat ccaatgaatt gattcaataa
    14221 ttgtgatagc ctgcctctat cgatgaaccg cctgaagctg aaggacctca cctatctcgt
    14281 cgcgctcgcc gaccagcggc acttcggccg cgcggccgag gcctgcaacg tgagccagcc
    14341 caccctgtcg gcgcagctga agaagctcga ggcgggcctc cgggtgcagc tggtcgagcg
    14401 cggcagccgg cgcgtcgcgc tcaccgccgc cggtcagcgc atcgtcgagc gcgcgcgcgc
    14461 gatgctcgag gcgggcgatg acatcctcga gatcgcgcgc agcttcagcg atccgctggc
    14521 cggccggctg cgcctcgcgg tgctgccgac catcgggcct tacctgctgc cgcgcgtcac
    14581 tcggcgcctg cgcaaggcgc tgccgcgact cgagctgatg ctctacgaat accagaccgc
    14641 gccgatgctc gagcgcctgc ggggcgggga gatcgacgcc ggcatcctcg ccctgccggt
    14701 cgacctcgac gggctcgagg cgaggccgct ctatgacgag ccgttcgtgc tgctcgcgcc
    14761 caccgggcat cgcctggcgc gcggcggaag cgtgcgcacc agcgatctcg ccggcgagac
    14821 cgtgctgctg ctcgatgacg gccactgcct gcgcgaccag gcgctcgacg tgtgcagtcg
    14881 cgtcggcatg cacgagaagc aggattttcg cgcgacgagc ctcgaaacgc tgcggcagat
    14941 ggtcgccgcc ggtgtcggca tcacgctgct gcccgaactc gccacgcgcg gcgcctatgc
    15001 ggccgcgcgg ggcaccgtcg cacgtccgtt cgccaggccg gcgccggtgc gcacgatcgg
    15061 catcgtgtgg cgcaggagca gtgcgcgccg cgcggccatc caggcggtcg cggacatcat
    15121 cggggcacac gcgctggcat aatccctcgg tgaccgacac ccaccccgcc gcgacccacg
    15181 tcacgccgta cgatcgtctc gaatcgcacg acgtcgccat tgagcggctg ctggtgagcg
    15241 gcgagcaacg cgagacgctc atcgactact tcggcgccgc agcctatgtg gaactctcgg
    15301 tgctcgcgcg ccgcgcggcg gagaccgcgc ggcggcgcgg cccgcgcgtg cacatcctgc
    15361 ccggcatcat gggctcgcag ctcgggcgcc cgcgcggcca tggcctgccg cacgacattc
    15421 tctggctcga tccgatcgac gtcgcgatcg gtcgcctgcg ggagatcacg ctgcgcccgc
    15481 actcgccggt gcgcgcgatg ggcgtgatct tgtacaactt cctcttgctg aagctgcggc
    15541 tcgcggtggc aggcttcgcg ccgcgattct tcgattacga ctggcggcgc agcgtcatcg
    15601 acctcggtcg cgcatttgcc gagcgcctcg atgaagacgg cagccgcgac gctcacgtcg
    15661 tcgcgcacag catgggcggg ctcgtggcgc gcgccgcgct cgcgctgccc ggtgaggcac
    15721 gcatcgggcg agtcgtgatg ctcggcacgc cgaactcggg gtccttcgcg ccggtgcagg
    15781 cgctgcgcgg cacctatgcg gtcgtgcgca agctcgccac gctcgatcag gtgcacagcg
    15841 cggaaacact ggcgagcgag gtctactcga ccttcccggg cctgtatgag ctgctgcccg
    15901 cggaaggcac caccgccatc gacctgttcg acgcatccgc gtggcccgct gccgggccac
    15961 ggcccgatgc cgatctgctc gccggtgcgc gcagcgcgct gcgcggcctc gccgaccccg
    16021 atccgcgcat gacgctgatc gccggccacg cccgcccgac cgtcaccggc gtgcgccgca
    16081 ccgcgcgcga cttcgagtac ctggtcacgc gcgacggcga cggcacggtg ccgctcgcgc
    16141 acgccgaact gcccggcgtg cgcacctact atgtccgcga ggggcacagc gagctcacca
    16201 gcaatgcgac ggtcgccgcg gccatcatcg acatcctgcg cagcggccgc acgcgacgcc
    16261 tgtcgtcgca gcggccggcg ccgacgcggg tggcggcgcg catcgacgat gccgcgctgc
    16321 gccgcacgca ccggcgcaag atcgactggc acgcgctcac gcacgacgag cgccgcaatt
    16381 tcctcgcgac gctgaacgat ccgccgaaac tgccgttgcg ggccacgcct gcacgccgcg
    16441 acgcgaagag cgcaggtgcg aaacacgccg gtgcgaagcc ggcgcgcggc gcgctcgaga
    16501 tcgtgatcac gcgcggcgac atccaggacg ccgacgccgc cgcgctggcg gtcggcgtat
    16561 accgcggcgt gcgacccgcc ggcgcagcgg cggccatcga cctgcgactc gatggcgcga
    16621 tcgccgagct cgcgtcgcgg cgcatgctgt cgggcgatgc cgggcagatc actgcgctgc
    16681 ccgccgcggg ccgcctgccc cacgcgcagc acgccgtgct ggtcggcctc gggcgcttcg
    16741 ccgatctcga tgccgccgcc atcgagcgcg cggccggcag cgtcgcgcgc tactgcgggc
    16801 ttgcgcacat cgcctcgctc gccactgtgc catggggtgc gggctccggg ctgccgacga
    16861 ccctgtcgtt cgcggcgcag ctgcgcggtt acctgcgaca ccgcgcagcg cccgggcacc
    16921 atctcgagcg catcgtgttc tcggtccgtg acgcggccga acatcgcgcc atcacgcagc
    16981 tcgccgccac gctcgcgcgc gaagtggacc cggacggccg ccagctgttc gtgcgatcgg
    17041 tcgcggcgcg ccctgccgcg cgcgcgccac gcaaggcggc ggcactggcg ccacgtctcg
    17101 cgcacctgct cgtcgagcgc accgcccagg gcagccgcac cgacagctgg cgcgccgcag
    17161 tgctcaccgc cggccagcat gccgcggtca tcgccgagac gatcgatatc gcgcgcgcgc
    17221 aggtcggcgc gctgctgcgc gagctcgaca ccgggcgcct caccgcagcg cgcgtcgcgg
    17281 gcatcggcgc gcgcctcgca aagctcgcgc tgcacaggaa cgtgcgcggg gcactggagc
    17341 tgacccgcaa cgaaccgctg gtcgtcgtca acgacgccga gacgagccgc ctgccgtggg
    17401 agatgctggc gctcggcgag tggtcgccgg cgatcggcgc cggcctgtcg cggcggctgt
    17461 cggcgccgga cctcagcgtc gcgcgcttca gcgcgcagcg gcgcgccgat cgcacgctca
    17521 ccgttctgct ggtcgcggat cccaccgagg acctgcccgg cgccgcacgc gagggtgcgc
    17581 gcgtgcgcga catcttccgc ggcctcgcgg atgcgcgact cacgatgatc accggcaagg
    17641 cggcgacgcg cgcgcgcgtg ctggccgagt tcgagtcagg cgactacgac gtcgtgcact
    17701 acgcggggca cgcagccttc gacccgcgca ctccggcggc gagcggcctg cacgtcagcg
    17761 acggggtcat caccggcggc gacctcgact cgctcgccca gttgcccgcg ctcgtgttct
    17821 tcaacgcctg cgaatcggcg cgcgtgcgcg gcgcgaagac gaccggccgc gcgacctcgc
    17881 tcgccgccgg cacgagcgtc gcgcaggcct ggctgcaggc aggcatcgcg aatttcatcg
    17941 gtacttactg gccggtcgcc gaccaggcgg cggagacctg cgcggggact ttctacaccg
    18001 cactcgtcgg cggcgatccg atcggcgccg ccctgctcgc ggcacgccat gccgtgcgcg
    18061 cccagcgctc gcccgactgg gccgactacc tgcactacgg cgatccgctg ttcgccctca
    18121 agacggcgcc ttgagcgcct ccacaatgcg catgcgctgg ctcgcgtcag gcaggcgccc
    18181 gcgacccgcc gccaggttgt cgcgctggtt cgccaccttg ccggtcgccg ggatcacgca
    18241 gttgacggcc ggatgcgcga gcacgtactt gagaaacacc tggccccagc tcgtggcgtc
    18301 gaactcggcg gcccactccg gcagcggacg accacgcacg atcgcgaaaa tcctgccgtc
    18361 ctcgaacgca cgattgacga tcaccgcaac accgcggtcg gccgcgagcg gcaacagccg
    18421 ctgctcggca tcacgtgtca ccgccgagta gttcagttgc acgaaatcga gcttctcgcg
    18481 cccgatgacc tcggcgagat cggcctggcc cgccacggtg taatgcgtga tgcccatgta
    18541 gcgcacccgg ccctcggcct tccaggcccg cagcgtcgcg agctgcgtgt cgagatcgcg
    18601 caggttgtgc acctggatca ggtcgagcgg ctcgtgcttc agcaggcgtg ccgactgctc
    18661 catctgcgcg atgcccgccg cggcgccgtc cgcccagacc ttggtcgcca tgaagcattg
    18721 cttctgctgc cgcgctgaga gcaggtcgtc gagcacacgc tcggcgctcg aatacatggg
    18781 cgaggtgtcg atcagccggc cgccgccctc gaagaacgca tcgatcactt tcctgagcgg
    18841 cgcacgcgcc gccgcgtcgg tgccgacctc catcggaccc gaagtgccga ggccgatcac
    18901 cggcagcgcc tcgccgctcg aggggatgcg gcgggtgatc agccccgtcg ttgccggcgc
    18961 accggttgcc gtggcatccg ttcccggcac ggtgtccgcc gcgaaggcgc gcgcgcttcc
    19021 tgcggccgcg acgcccgcgg cggcgctggc ggccgcaacg atcgcgttac ccaacaggtg
    19081 tctgcgattc atgagctcgt ccctcgggcc cggaattgcg cgcccttccg gtgactctat
    19141 caacttgcag gcagactcgt cgatgggagc cggggactgc tgctcgtcag gagttggttg
    19201 gatcgagcgg ctcgtcagtg aatctctcgt agagctgccg tagtcgtcgc ttgccaatgc
    19261 gaaactgact acgtgccttc accttcgatt tcttggtgct cgcatcgacg accttcgagc
    19321 ccggatcaaa ggcaactgcg ccatcgacga gcgcgcgcag aaatctcaca aactccgtcc
    19381 cttcgccaag cgaggccgga ctcagatagc gatagcgtgc gcctgccgct tccttctcat
    19441 agggaacata ggcagcggct gcgtgcttgc ggccccagtg ttcaagcagc ctgctgaagg
    19501 accaaccggc actctctgaa ccattcggtg caatgagata cacgccaccg ccgacatcaa
    19561 cgatctttcc ggaggcgata tcaaagcccc gaacggccaa ggtctggccg gtcgcccggc
    19621 acaattcacc aacccggtgc aggccggtga aatagagagt gtctcccccg gcctgatgac
    19681 cgaacttgcg cacgaattcc tctgcaccat gcttgccata gaagcctgag tccggctcgg
    19741 gcgtcatcag ggtaattctg tccgaaccgc aagccttcag ttcccagccg agaaaatcgg
    19801 gctgcgcctt gctgttgggg acgattccaa acagcgcctc gagcgtgtag ccggcaccgt
    19861 tgcgtgcaat gtaaggctga gccacgcgct gcgcgttcaa gcgacgcgag gcatgccatc
    19921 ccgcggcatg aatttcccgg aggcgggcga gcagtgcact tcgcgcatcg ttcccctgca
    19981 atggaatgtc gaggaatacg cctcgttgcc tcggccgcca gcgcattgcc tggttctcga
    20041 actcctccgc aatcgctgaa cccgcagggg caaggaaagc gatcaggtga cgatcggggt
    20101 gattcgcaag aaagaggatc cggccatcgg gagcattgtc gaacttgcgc cgctcacgcg
    20161 ggacgggctg caagaacgcc gcgggcgccc tggcgcagcc gcgcaagaag ccggagaggc
    20221 gcacttccgg gtagtcggga tacaggatga gctgcgctcc ggaggccggc gctgtgcgac
    20281 ctgcttcgtc gagccacaac agcttgagcg gtgccttcat attcggtcgt ttcgccgatg
    20341 cgtcgctcgc gacctcgccg aaaggcagca tgttcaatgc atccaggctg ccgcccaagt
    20401 agatctgttg tttgctgttg tcattctccg caaggcgctt gcagaaagta tgtgtggcgc
    20461 ccaggtcccg gaatgcatcg aacagagctt caagcgtgcg tatggtcatc gcagccgcga
    20521 gccccggatc tccgcgtacc tgcgacgccc catgatccac gctgacgccc gggatacagc
    20581 cttttctgag gcatcgcgcc cgaggccctt gaaagcacat tcccagatca cgagttgacg
    20641 ccagccggct tcgtgaactg cgcgagtcac ctccgcgtca cgacgtcgat tcgcgcggat
    20701 tttgtcactc cagaacttgc gtctggtgcc cggcatccgg aagagatgac agtcgtgccc
    20761 atgccagaaa cagccatgca cgaagatggc cgcgcgatac ctaggcagca ccagatcggg
    20821 acgacctgca ggctgcgctg catgcagccg gaaccggaac cccagccgat gaagcccgct
    20881 gcgaatgatc ctttccggtc gggtattgcg ggcgcgaata ccggacatca tccggctccg
    20941 ggtcgccgcg tctaccacat caaccaaaat tcgcggcctt gcgtgcgctg tagaaaagat
    21001 cctgcgaggc ttcaccggta atccagggct tcatgtgctg cgcaactgcg cgcacaaccg
    21061 gcacaacgac cgcatttccg aactgcttgt aggcttgggt atccgagaca ggaatgcgaa
    21121 acggtgaccg cccggccgca tcgaaaccca tgagccgcgc gcattcgcgc ggtgtcagcc
    21181 gtcgcggtgg cttgcccttc tgcctgacga gtatttcgga tccgtctttg taatagcgcg
    21241 cggacagtgt gcgggtcaca tcctgcggac ccacaagtcc gaatccaaag ccgttaccct
    21301 tctgtcgatg cttctcggca tagttctgca agtaccgcca cagatgctcc gtgagcgtgt
    21361 atttcggcag tacttttccg cgcggacctg cgatatagcc gatcctggga tcgtctatgt
    21421 cctcggtgcc atcttcagga tgcagaacac tctcgaggcg aggacccttt gccggatccg
    21481 gcgcgacaaa atcgtcgaag gtgaatccgg tctggtcacg gaaccccacg atgaatatgc
    21541 gctcgcgatg ctggggcacc catgaacgcg catcaatcac acgcgcatca atgtgatagc
    21601 cgagctcttt cgtgagtgct ttcgcgatca cacgaaacgt attgccgcga tcatggctca
    21661 ccaagttctt cacattctcc agcagaaatg ccttcggccg atggtgctcg atgatgcgcg
    21721 cgacatcgaa aaacaatgtc ccctgcgcgt cacaacggaa accatgcggc ctgccgagcg
    21781 cgtttttctt cgacactccg gcaatcgaga aaggttgaca gggaaaaccg gcaaccagaa
    21841 cgtcgtgctc gggtatcgcg gacgcggcga cggtgcggat gtcgcctccg atttcatgcg
    21901 tatcgtcagg aaaattggcg cgataggtcg cctgcgaata tttattccat tcgttcgtat
    21961 agacgcagcg cccaccagcg ctttcgaagc ccaagcgcat gccgccgatg cccgcaaaca
    22021 ggtcgatgaa tctgaactcg gttgagggcg gcgacgcgac ctcccttacc gaaagattcc
    22081 gcaaaatctc gagcgcagcc tttcttggca cctgatcgcc gcggtcccag cggtacaccg
    22141 tgctttccga aaagcccacg gcctctgcgg ccattgcgat tgaaaggcca gcacgctggc
    22201 ggaggtggta aaactcactc aaggaggtgc tccgcggggt cattttttgt cactatgcca
    22261 acgtgtggcc cagagtgcaa tctgtaaaac catggcgaac gcccggatat cggctcgtca
    22321 gggatgcggg ataggtcgcg ccgcgggact cgatgcgacg cctccccgag gccgccctgt
    22381 caaaatgcga gcagaatcgg gcagtttgac cggttgttga caccgctgcg ccgcgggcac
    22441 cttgcgcggg ccggggcagt cacctcaggc agcgcgacgc ggcgcaatgc cgcgcctcag
    22501 atcagcgccg cgtccacctc cgcgaccgac gcaccgccat gctgcacgat acgtgcaagg
    22561 ctgtcggcgg tcggcagaac ctgcgcggca tagaagcgcg ccgtggcgag cttgccgcga
    22621 tagaaatcgg catcggcggc ccccgcggcc agcttgttcg ccgcaatcgc tgccgacctg
    22681 gcgtgcatcc acccgccgag caccgtgccg gcaagctcga ggtagggcac cgccaccgac
    22741 agcgcgaggt cctgcgcact gccgagctgc gcgagcagcg cctgcgtggc ctgcgacagc
    22801 tccgcgacgc cttcgagcgc cgcccgcttc gcgacctgca cggcagcgtc gctcgagtcg
    22861 atcgcctcga gttcgcgcgc gacgtcggcg agcagcgccg ccatcgcggc gccgcggtcg
    22921 cgaccgatct tgcggccgat gaggtcgttc gcctggatgc ccgtcgtgcc ttcgtagatt
    22981 gtggtgatgc gcacgtcgcg ataggcctgc gcggcgcccg tctcctcgat gaagcccatg
    23041 ccgccgtgca cctggacgcc gagtgaggcg gccctgatgc caccctcggt gctccaggcc
    23101 ttcacgatcg ggatcagcag gtcgccgcgt gcctgcgccg cggcgcgcgc cgcctcgtcc
    23161 gagtgggccg cggcgatgtc gagctgggct gcggtgtaga tggccagcgc gcgcatcgct
    23221 tcggtgttgg acttgatcgt gagcagcatc cgcttgatgt cgggatggtg gatgatcgac
    23281 gccgggccac tcgtcgcggc taccggcggg cgcccctgca cgcgcgtgcg ggcccactcc
    23341 gcggcctgct ggcaggcgcg ttccgccacg gcatagccct cgagcccgac cgacaggcgc
    23401 gcggcattca tcatgatgaa catgtactcg aggccacggt tggcctcgcc gaccaggtag
    23461 cccaccgccc catcgcgcgc accgtactgc agcacgcagg tcgggctcgc atggatgccg
    23521 agcttgtgct cgatcgagat gcactgcacg tcgttgcgcg caccgatcgc accatcgtca
    23581 cccaccatga acttgggcac gatgaacagc gagatgcccc tggtgcccgg cggcgcgccg
    23641 tcgatgcgcg cgagcaccat gtggatgatg ttcgacgtga aatcgtggtc gccccaggtg
    23701 atgaagatct tctggccgaa gagccggtag tggtcgccgt ccggggccgc gcgcgtgcgg
    23761 atctggccga ggtcggaacc ggcctgcggc tcggtcaggt tcatcgtgcc ggtccactcg
    23821 ccgctcacca ttttcggcag aaagcgctgc ttctgcgcat cgctgccgca cagctcgagc
    23881 gcttcgatcg cgccttgggt cagcatcggg cagagcttga aggcgagatt cgcgctcgcc
    23941 cacagctcct gcaccgcggt cccgagcatg cgcggcatgc cttggccgcc gtactcggga
    24001 tcccccgaga gctggttcca gccgcccgcg gtgaaggcct cgtaggcggc cttgaatccc
    24061 ggcgtggtca ccaccccgtc gggagtccac ttcgcgccct gctggtcgcc ggcgcggtac
    24121 aggggatcga gaacttcttc ggctaatttg ccggcttctt cgaggatcga gctggcaagt
    24181 tcggcggaat agtcggtatg tcgcgggcag ctcgcgagcg cgccctcacc gatcaggtca
    24241 ttcagtacaa actgcaggtc tttgagaggc gctcgataca taccagttcc ttgagctcac
    24301 ggatggtgct gtgcgggacc tgcattgtaa gcccgcgagc cgcgcgccga gcgccgagga
    24361 ccggtcagcc ggtcgcgagc gcagggatca cgtttgcggc caagaactcg acatacacct
    24421 gcacacgcct gtcggtggcg agcgttgccg ggtagatcgc gcacagcggc atggtggccg
    24481 gcagcgacca gtcgggcagc acccgcacga gcttgccggt gacgagcccc tggcgacaga
    24541 ggaactccgg cagcaggccg atgccgaggc ctgccgcggt cgaggcatgc agcactgcgg
    24601 gatcgttcac cgcaagcttg ggatgcacgc tgacctcttc ccgctgcgaa gtcccgcggt
    24661 gcaggtgcac gcgatactcg ggccccgtgg cggcgggcgt cagcagcgcg tgccgcgcga
    24721 ggtccgcggg cttcgcgggc gtgccgtgcg cctgcaggta agccggcgtc gcgcacagca
    24781 ctgccggcgg cgcgccgagt tcccggcaca cgaagcgcgc atcgtcgatg gcgcccacac
    24841 gcacggcaac gtcccagtcc gtcccgttct cgagatccac cgcctgcatc acgacgtcga
    24901 gctgcacgtc gggaaagcgc tcgagaaagc gcggcacgat cggcgcgacc agcacccggc
    24961 cgtaggtggg atcggccatc acgcgcagcg gcccgccgcg caccgcgatg gcactggccg
    25021 ccgcggcgcg cacggcatcc gcctcatcga cgatgcgacg gcaatgcgga tagagcagtc
    25081 gcccgacggg cgtcagatgg atacgccgcg tcgtgcgctc gagcagacga acgccgagcg
    25141 ccgcttcgag ttcggcgatt gcccgactgg ccgcggcctt cggtaccccg agcgctcgcg
    25201 cggccgccga aaagccgtta aggtccacga tccttgcgaa cagcgccatc tgcgccggca
    25261 tccagattgt gctcatgggc attactccac cggcaaccag ggcagtcatt gtttcactag
    25321 taggatgtag tatgcgcctc cgcaagctcg gtacacaggg tctcgaggtt tcggccatcg
    25381 gtctcggggc catgggcatg gcgggcgtcg ccggcatggc cgacatgtat ggcgcggtcg
    25441 acgaggccga ggcgatcgcg accctgcagc gggcaatcga catcggcgtc aacttcatcg
    25501 acaccgccga ggtgtacggc ccgtacacca acgagtcgct cgtcggccgc gcactctcgg
    25561 ggcgccgcga ccgcgccatc atcgcgacca aattcggctt tcgcctcgat ggcggcaatc
    25621 gcctcggcgt cgacggcagt cccgccaacg tgcgccgcgc gatcgacggt tcgctcgagc
    25681 gcctcggtgt cgatcacatc gacctgtggt accagcatcg ccgtgaccgc gacgtgccga
    25741 tcgaggacac ggtcggcgcg atggccgagc aggtgcgtgc cggcaaggtg cgctacctgg
    25801 gcctgtcgga agtcggcgcc gagacgctgc gccgggcaca cgccgtgcac ccgatcagcg
    25861 cgttgcagag cgagtactcg ctgtgggagc gcaatatcga ggacgaggtg atcggcgtct
    25921 gccgcgcgct cggcatcggc atcgtgccgt acagcccgct cggccgcggc tttctcgccg
    25981 gcaaactgcc ggatccggcg acgctgccgt ccggcgatta ccggcgcaga ttcgatccgc
    26041 gcttcgagcc ggacaactac gctcgcaacc tcgccatcgt gcgggaggtc gagcggatcg
    26101 cgactggcca cggcgccacc gcggcgcagg tcgcgctcgc ctggctgctg caccagggcg
    26161 atgacatcgt gccgatcccg ggcaccaagc ggcgcaagta cctggaggaa aacgcggcgg
    26221 ctgccgatct cgcactcgag gcggcggacc tcgcggcgct gaaggcgctg cagcccgcag
    26281 ccggcccgcg ctacaccgag acggcgatgg cgtccatcga acgctgaata tgacaaatcc
    26341 ggccgtgcgc gctcacgaca gaacgcgagc gcgatcacgg cgcgcgtgtg ccgttcctgc
    26401 gaaccttggc cggcggctga ctgccgcact gctgcagcct ttccggggac acacacgaac
    26461 atgttcaagc tgagacgcgc caagcccgcc gcgcgggcga aagtcgaacg gcccctggcc
    26521 gaacgtgagc gcgtcacacc ggaggtcctg ggccgcgaat tgcgcgaggc attgcgccag
    26581 atccgggtgc actcgatttc gatccacaac gacgagtgcg atgcgctgtg gctcagcgag
    26641 ggtgtcctcg gccccgacga gcattcggtg gcactcgagg cgatgggcgc cttcggcctc
    26701 gaaacgtccc gtcagtacgc cgacttcgac ctcggcgacg gacgcggcgc cgtgtgctat
    26761 cccgcgcgct caccgtatgg tgaagtgctg ggcctcgcca tggtgatcgc cgacaccaaa
    26821 tcaatcgaga acggcaacgg cgaacggctc gccacgcccg aagtgcgtgc gatcctgcag
    26881 cgcctaacgg tcgtgctcag gccgccttct gccacgcaga cacaaccgat cgcgcggccc
    26941 gacgccctcg cgatcgccgc cgccggcacc gcttcggctc ccgcggccac cgtcgccctc
    27001 tcgccggtcg ttacggtccg gccggcggcg gtggtcgctc cccctgcccg cgaggccacg
    27061 ccgcccgcac cggcgcccgc gcccgcggcg gcccaggtgc cggtggtcga tcccgcggcc
    27121 agcgattgcg atatgccggt gatcgagttt tccttcaacg acctggccga agacctggcc
    27181 gacgaaccgg tcgtcgcccc cgctgcgacc ccagcgcccg ccgcccggcc ggcggacacc
    27241 tcggccgatg cgacgctcta tgtgccgcag gcgaccgtgc gcgaagtggc gctgcacgtg
    27301 cagcagctgc acaagctgcg ctcgggcggc cgcacgcgcc gcttcgaggt gctgctgcgc
    27361 gcgcgcagcc accccgacgc cgatgcgatg accgacacgg tcgccgaaat gctcgcgtcg
    27421 cagggcacga cgagcacgat ggaccgctac atcacgggcg agctgctcac ctggctcgcc
    27481 tcgcacaccg aagtatggga gcgcgaaccg gcgagcttct cggtcaacgt gacgctctcg
    27541 acgctgaccg acgagcagtt cctgccctat gtctcgagcc ggctcgcgga agcgcgcgtc
    27601 gccccggagg tcctcggttt cgaggtgccc gagcgcgcct tcatcgagca tcgcgcgctc
    27661 gcggagcgct tcgtcagggt ctgcgagaag ctcggctgtt tcgtcgtgct cgacgatttc
    27721 acgctgcact cggaagcgct gcccttcctc gcctccccgg cgatgcgcct cgtgaagatc
    27781 gatgcgcgcc tgacctcggc cgcgctcaag gagaagctgc cgcaggcgat cgtcatcgcg
    27841 atctcccagg cagccaaggt gctgggcgta cattgcgtcg ccaagcgcgt cgagtcccag
    27901 gtgggccggc agtggctgtc ggccattggc atcgacttcg cccaggggtt cctgctggag
    27961 aagccgcagc ccctcgacga cctcgccctg caggccgtca aggccgccag cgagggctga
    28021 accgcccggc ccggacccgc cccggcccga tgtgcgacga agcgagggcc gggcacgacg
    28081 tcccgatact cccggggcga cgagtcgcat aaagtccacg ccatgagtgt tgcaatgccc
    28141 actgcggctg cgccagaggc cgcgacacgc ctcctcggtg gcctgcgcat atcgcgaaac
    28201 accctgttct ggtgcctgca cgccgccgga tggttcgcgt atggctgcgt gcagtacctc
    28261 ggctcactga tctacgaaaa ggagacgggc taccagctcg tgatcctctc ggcggcgatc
    28321 gccggcttcg tcgtctcgat gccgctgcgc tacatctatc ggatgctgtg gcgccagtcg
    28381 ccgcgcgcga tcctgctcgg catcctggcc acctcctacc tgaccgcgct cgtgtggcgg
    28441 ctgtgcgtca acttcgcgta cgagcggttc atgccggaga tggactggga gataacccac
    28501 tggtaccagt acttcggcgg cgccaaccag ggcacctacc tgatgctgtg ctggtcggcg
    28561 ttctacttcg gcatcaagta ctacgaaatg ctgcaactgc agcgcgaggc cacgctgcgc
    28621 tcggccgccc tcgcgcagga ggcccagctg cggatgctgc gctaccagct caacccgcac
    28681 ttccttttca acacgctgaa cgccatctcc acgctgatcc tggacaacca gaaccgcacg
    28741 gcgaaccaag cggtcacccg cctgtctgat ttcctgcgct acacgctcga ccaggacccg
    28801 atgaagaagg tcactgtgcg ccaggaactg gatgccctga acatgtacct ctcgacagaa
    28861 cagctgcgct tcaacgaccg cctgcgcatc gagttcgcgt tcgaggagcc ggccctggaa
    28921 gcgctggtgc ccagcctgct gctgcagccg ctgatcgaga atgcggtcaa gtacgccgtt
    28981 tctccccgcg agggcggcgg cacgatcagg gtcgaagggc gcgtgcgcgg cgccatgctc
    29041 gagctcaccg tcgccgacga cggcccgggc gcccgaacct cgagcgcgct catcgagggc
    29101 cgcggcgtgg gcctgcgcaa tacgcgcgag cgcctgcacg tcctctacgg cgatacccac
    29161 cggttcgcgg cgatcaatgc ccaccccggc gtgcgcatcg agatcgccct gcccttagag
    29221 aaggcatcga taccatgacc aactcacaga tccgcacgct gatcgtcgac gatgaggcgc
    29281 tgtcgcgccg gggcctcgag attcgcctgc gcacggccgg cgacttcgat gtcatcggcc
    29341 aatgcaccaa cggtcgcgag gcgatcgacg cggtgcgcaa ccagcagccc gacctcgtgt
    29401 tcctcgacat ccagatgccc ggcctgtccg gcttcgacgt gctggcgcaa ctgcccaccg
    29461 actcgctgcc gatggtcgtg ttcgtcaccg cctacgacca gtacgcgatc cgcgccttcg
    29521 aggcgcgcgc ggtggactac ctgctcaagc ccgtcgacga gtcgcggttc gccgccgcga
    29581 tagagcacat ccgcgaactg cgccagcagc gcaacgccgc ctcgcaacgc gaccggctgg
    29641 tcgaactgct ggccgacgta tcgggcacgg gcgagatcga cgccgagggc ctgctggcga
    29701 ggggcgcggc ggcgcatgcg gcggaacgca ataccgtgct gccgatccgc gaggggcgcg
    29761 agacggtccg cctcgacacc aagtcgatcg actggatcga cgccgcaggc gactacatgt
    29821 gcatccatgc cgggccccgc acgcacatcc tgcgcggcac gatgaaggaa ctcgaggacg
    29881 tgctcgaccc gcgccacttc cagcgcgtgc accgctccac catcgtgaac ctggcgcgtg
    29941 tgaagacgct gcgctcgcac atgaacggcg agtacttcct gacgctcgac ggcggccacg
    30001 aactgaagct ctcgcgcacc taccgcgaca aggtcgaata cttcctcaag ggaccgccgc
    30061 ggctgaactg agcgggcgcg gcggcaggta ggtggcgtac cagtcgaggt agcgctgcag
    30121 ccggtcgacc cgcaggctcg gccggccgag gccatgcttc tgacccgggt agatgacgag
    30181 ccgggtcggc acgccgacgc gccgcaatgc ctggtacatc tgctcggtcg cggcaagcgg
    30241 cacgttgaag tcgtcctcgc cgcatagaaa cagcgtcggc gtgcggatcc ggtcggcgtg
    30301 caggaacggg tacgacaacc gcagccacag gtcggtgttc tgccacggca ggccgagctc
    30361 ggcttcccat tcgcgcaggt actgatcgag cccaaagccg ccgagcatgt tcgacatgcc
    30421 ggcgccgctg gtcgccgcct tgaaacgcgt gtccgcggcg atcacgtagt tggtcaggat
    30481 cgagccgtag ctccagccac cgaccccgag gcgcgccggg tcggcaagcc catcggcgag
    30541 cgcgcggtcg gtgatcgcga gcacgtcggg tacgtcggcg gcgccccagt tggcgaacag
    30601 cgcgcgctgg taggcatagc cgcgcccgct gctgccgcgt ggattcggcg cgagcaccgc
    30661 gtagccgtgc gcggcgaaca gctgcagctg caggtcgaac tcgtgctgga actgcgacac
    30721 cgggccccca tgcaggcgca gcagcgtcgg atggcgcgta ccggccggcc ggccccgcgg
    30781 ctcgtagacg agcgcatgga tctcgaggcc gtccgcgctc tgcgccgtcg tcgtgcgcgc
    30841 ggtgacgaga tcgacaccgg cgagccaggc gtcgttgtga tgcgagatgc gccgcaggcg
    30901 gccgcgttcg agcgccacga gttcgccggg ttgcgcactc gtaccgtgca ccatcacgac
    30961 gcggccgcgg acacccacgt cgtattcgct gacgacactg cccggcgcgg tgagccgctc
    31021 gatgcgaccg tcggcgagac gcacgcgcgc gagcaccatg ctcatgtcgt cctcgaggcg
    31081 gaagtagagc cagcgcccgt cggccgacca gcgcggctcg agcgtgttgc gatcgagcac
    31141 cgcgctcggg agccgcgccg cggcgggggc ctcgcgcgcg ccggccggca cctcgatcac
    31201 ggccgcctgc agcaggccgt accagatgtc cgcgggtgcg ccgccggcga ggtacgcgat
    31261 ccggcgccca tcggggctgt agcgcggcgg tccgctcagc cagtcctctt cggggtcacc
    31321 gtccgtgccc ggatgggtgg tgagctggcg agcgccagcg cccggctgcg gcgccacgac
    31381 atacaggtcc cagttgccgt gcgcatcggg cgcctcgccg cgcttgctga cgaacacgat
    31441 ctgcgtgccg tccggtgacc aggcgggctg cacatcgtcg aaggcgccgt cggtgagctg
    31501 ctgcgtggcg ccgctttcca cgtccagcag gtacaggtgc gagcgttcct cgcgcagata
    31561 gcccgccgca tcggacttga accacagccg gtcgattacg atcggcgcgg gcaggtcttc
    31621 gtccccgtcc tcgtagcgcg gcaggcgcgc gctgaacacg atgcggcggc cgtcgggcga
    31681 ccacgcaaag tcggtgatcg cggtctcgaa ccggctgcgc tgctgcgcct cgccgccggc
    31741 catctcgagc acccagatct gatcgcccgc cttctcgtcg acacggtcgc tcaggaacgc
    31801 gagccgccga ccatccggac tccagcgcgg gctgtgctcg ctcgaggtgc tgcgcgtcac
    31861 ctggatcacc tggctgccgt cgtagcgtgc aagccagatg tcggtctggt ccgcgtccgc
    31921 gtcgcgatcg gtcgtactca ccgtgtaggc gatccactcg ccatccggcg agaggctggg
    31981 gctcgcgagc tcctgcagct tgtagacgtc ctcggcgacg atgccgcgcg ccgcggcact
    32041 cgcgcagggc agcgccgcga tcgcggcgac gacgactaga agtcggcggc cggcgtggcg
    32101 cggcggcgcg ccggactgta gaacggaccc tcgacgacgg tacagcgcgc catgaaccgg
    32161 ctccatttga gttccttctg gtaatagatc tcggcccaga gctcgccacc cggcgcgcca
    32221 tgcggcattt ccacgctggc gagcgcgata ttcgccttcg ccgcgggcga ccacatcgcc
    32281 gaggtcacca cgccgacatt cctgcgccgc cggtcataga ggtaggcgtc cttcgcgggc
    32341 ttgttgccct cgacgtcgag tctcaccagc cgatggcgcg aaccgcgcgc ctgttcggcg
    32401 agcagcgcgc gccggcccgt gaaatgcggc ttgccgaagt ccaccagcca ggcgaggtcc
    32461 agctcgaacg gcgagcgcgt gcggcccgcg cgcacggccc ggtcggccgg cagaaaatcg
    32521 acccccgcct gaatgaagcc cgcctcgatg cggctcatct cgagcgcatg cgtgcccatc
    32581 ggcttgatgc cgaccggcgc cccgacgtcg aacaggcggt cccacagtgc gatcccgagt
    32641 tccgggtcga tccagagctc gtagccgagg tcgccggtga agccggtgcg cgagacggtc
    32701 agttctccgc cctcgaaggc gaagctccgg atgccgaagg gcgtgagcgt ctcgatgccc
    32761 gcgaggccga ggcgcttcag caccgcgcag ctcgtcgggc cctgcagcgc gagcgcggcg
    32821 acgtcgtgcg tctcctcctg gatcgccgcg tcgaagccga gcgccgaggc atgcagccag
    32881 tcgagctggc gctcctgcga gcacagccga tacacaccat cgcgcaggtg gaagatcgtg
    32941 ccgtcgtcga ccacctggcc ggcgtcgtcg caccacaccg tgtaacccac gcggccgggc
    33001 ttcagcttcg ccacgtcgcg ggtgaccagc cggttcatga acgcgagcgc atcgggcccc
    33061 gtgatgaggt gcttggtcat cggcgacaga tcgaacaccg tgcaggcgtt gcgcagcgcc
    33121 gtgtactcga gcgagacgtc gaagtaggcg tcggcgaccg tgtagtcgcg ccagcgatgc
    33181 cacacgttca gcgtgtttgc cgccgcggtg cgcgtgtgga acggggtcgg gaagaccgcc
    33241 tgccggtaat gttcacgtgg aatcgtcacg cgcgactcct cgcgagcacc acgcgcgcgg
    33301 cgttgcggcc ggccgcgccg atcacgccac cgcccggatg actgccggcc ccgcacagat
    33361 agagccccgg caccggcgcc ctgtagtacg cggcgcccgg cacgggacgc aacatcatgt
    33421 actggtcgag cgcgagttcg ccgtggtgcc agtgcccgcc gtggatgcga tagtcgttct
    33481 cgatgtcgag cggcgtcagc agctccgcgg cgacgatctg gctgcgcagg gcgggcgcgt
    33541 ggcgcgcgag cacgccgagc gtcgcctcga ggaagggcgc acggccggca gcccagccga
    33601 ccgcgagatc gcgcggtgcg tattgcacga tcgccgacag tacgtgctgt cccgggggcg
    33661 cgaggcccgg gtcgtgcatc gagggcacgg tgatctcgag caccggctgc gaggagtatt
    33721 cgcggtactt cgccgggttg aacgccgctt cgatgtagcc gaggtcgggg gcgatgacca
    33781 gccgctcgcc gagcagttcg gcgggcacgc cgcgaaaatc gggcgggcca gacagcgcaa
    33841 ggtgcagctt cgccgcgcag cccgtcgaac ggatgtgcgc cacgcggcgc gcgaactcgg
    33901 cgtcgagatg gcgcgcgccg agcagtccga ggaaagtggt ctggggatcg acattcgata
    33961 ccaccgtcgc cgcagcgatc tgctcgccac tctgcagcac gacgccggtg acccgctccc
    34021 cgtccagcat gaacgacgcg accgggctcg ccacgcgaat ctcggcgccc gctgcgcgcg
    34081 cggccaccgc gagcgcaccg gtcaccgcac ccatgccgcc ccgcggcaac ccgcgggcgc
    34141 cggcgcgctg caggctcgtg aagaaggtct ggcccgagcg tggaccgagg cgcgcaccga
    34201 gcacgccgtc gagcgcgagc gcgcccttga gctgcgcgct gtcgaaacga tcctcgagca
    34261 ggtcgtacag gttcatcgtg ccgacgcgga gcagctcccg catgtccgcg cggccgagca
    34321 ggcggacatc gagcgcgaat ctcgcaagcc acggtaaggg gctttcggta ttccggtttt
    34381 tcgcactccg gccgaacagc cgcaataccg aaaacccggc accgaacccg tgctgcttcg
    34441 cgagcagcgc ggcgaagcgc cgctggcgcc gcacgtagtc gacgaggccc gcacggtcgg
    34501 cggatgagac atcgccggcc agcacgttcg cgccgtcgag cacgagcggt gcgctctcac
    34561 cgagcgcgac ggtgagcagc tcgcgcctgg cataagccag cccgtgctgc gcgagtcgca
    34621 gctcgcgctc gatgcgcgga tcgagcgcat gcagcagatg cgccaccgac gagaccctga
    34681 agccgggcgc gaactcgcgc gtcaccgcgg caccgccgac ctggtccgcc gcctcgagca
    34741 ccagcacctt gcggccggc
//
LOCUS       DAOQVY010000008        34177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_1470_length_34177_cov_5.185042, whole genome shotgun sequence.
ACCESSION   DAOQVY010000008 DAOQVY010000000
VERSION     DAOQVY010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 34177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34177
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1470_length_34177_cov_5.185042"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PKN91_01605"
     CDS             <1..703
                     /locus_tag="PKN91_01605"
                     /inference="COORDINATES: protein motif:HMM:NF019944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HNS26794.1"
                     /translation="VPGTALAPGVQPSGAQPAAATPGGPDNLSRQSTRNYEIDRTVAY
                     TRQPAGRLQRLTVAVLVDDLIVPQKDGAVVQKPLSEEQLARITLLVKDAVGFDAARGD
                     SVNVVNASFATGADQPADIVEEGIPLWRQPWLQELVKIIAGAAIVIVLLLAVVRPLAR
                     GLLGPVRFAPGTAPALPAAGGEAVATADEDTPRGAGQVSAIAYEQQLAQARSLVTQDP
                     KRVARVVKTWVGQDE"
     gene            696..1709
                     /gene="fliG"
                     /locus_tag="PKN91_01610"
     CDS             696..1709
                     /gene="fliG"
                     /locus_tag="PKN91_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNS26795.1"
                     /translation="MNEKGLIARSSTDQAAILLLSLGEQEAAEVLKHMSAKDVQRVGT
                     AMAALDGVTREEVSQVLGHFTTLVESHTSLGVGADEYLRKVLISALGDERATNIMDRI
                     LGGRSSRGLEAMKWMDPRSVAEMMRFEHPQIVAIVLAYLDPDQAASVLGQLPEMARSD
                     ILMRIAMLDGIQPSALNELDEIMEKQFAGNSSAKASVFGGPKVAASIINFLDSGQETV
                     IMERINRADEALAEKIQGLIFTFDDLVSLDDRGMQELLRQVPGDRLLLALKACEPGVK
                     DKVFRNMSQRAAEMLKDDLEAKGPVRVSEVEAAQKEVLATAKRLADAGTISLGGKGGE
                     AYV"
     gene            1702..2328
                     /locus_tag="PKN91_01615"
     CDS             1702..2328
                     /locus_tag="PKN91_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HNS26796.1"
                     /translation="MSDPARWELPVVEGRVAGRRARPITADELDDIARAAWDEGHAKG
                     HADGLAAAAAEVQRRHAALDARIARLDEIATLLARPLAELDAEVESQLAQLALTVAGQ
                     LVRRELRIDRTQIIGILRHVVSLLPAAARDVRVHLHPDDAALVNEKLATPASERAWVL
                     VEDPVMARGGCRVTAENSQIDARLESRLNATIVEVLGEERLGDGREQS"
     gene            2325..3686
                     /gene="fliI"
                     /locus_tag="PKN91_01620"
     CDS             2325..3686
                     /gene="fliI"
                     /locus_tag="PKN91_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HNS26797.1"
                     /translation="MTLLQERQLRWREGLARYQRRALALPPPPVEGTLTRVVGLTLEA
                     AGCEAAVGDRCEVLSTLGGKVEAEVVGFAGERLFLMPTADAHGLSPNARVVPRTGRQT
                     VRVGEQLLGRVIDGAGQPLDGLGALHCDAPVTLMGGAPINPLAREPITEPLDVGVRAI
                     NSLLTVGRGQRIGLFAGSGVGKSVLLGMMARYTAADVIVVGLIGERGREVKEFIERIL
                     GEEGRRRAVVVAVPADQSPLMRLHGAWLATAVAEYFRDQGKSVLLLMDSLTRFAQAQR
                     EIGLAIGEAPATKGYPPSVFARLPQLVERAGNGARLGGSITAFYTVLTEGDDQNDPIA
                     DSARAILDGHFVLSRRLAEAGQYPAIDIEASVSRAMHDIVAESHFEAARRFRHALSTY
                     TQNRDLISIGAYRAGSDPRVDAAIALWPRIQTFLRQHMRERADLAASLAALSEVTAEG
                     ADA"
     gene            3683..4129
                     /gene="fliJ"
                     /locus_tag="PKN91_01625"
     CDS             3683..4129
                     /gene="fliJ"
                     /locus_tag="PKN91_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HNS26798.1"
                     /translation="MTRARRMDPVVRAVGHDEREHAARLAECGQRVADAEQRLAELEK
                     YQADYASGLHSRTAAGISATEMRDFRAFLARLSEAVRTQQRLLAQARAEHEAAREAWR
                     RAAQRARSIDHVVERWQAEERIHRERREQIDIDERALQLTRARTRH"
     gene            4153..5094
                     /locus_tag="PKN91_01630"
     CDS             4153..5094
                     /locus_tag="PKN91_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNS26799.1"
                     /translation="MMTTAPVSPPAASTAPAESPTGADPALAGLFGEELAQLLLEEQG
                     GDDDEALAPEALALLLAAVVEAPPASSAAPAAADADSPMGGVLRRLELHGAPAAPVDS
                     GAEASLDGMLQELRTSTAGSRSLAESPAALTAQAGSTTVQIATTSATDHLAGLVAASG
                     QAADSRPTTQHAALRHELPLQTPVGSHAWGDELASRIATIATDGPQSASVRLSPEHLG
                     PLHIRIAVRDGDAAVTFGASHADTRAALEQSLPRLRELLAAQGLNLAEASVSEHAPRE
                     DRAPTPAGRRAVAAGADPVQDAGRREARPVGLVDLYA"
     gene            5260..5739
                     /locus_tag="PKN91_01635"
     CDS             5260..5739
                     /locus_tag="PKN91_01635"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNS26800.1"
                     /translation="MVEAAVSSPPRKSSKALLWIVLVLLVGAAGAAGSWFYLGRSAAP
                     AAAAVPRPQPALFHQLDPSFVVNFEAEQMVRFLQVTIEIMTRDPAALEFVRQHEPVVR
                     NDLLMLLSNQKYEVLATQAGKDELRARALEAVRAVAAREGGDPAKLEAVYFTSFVMQ"
     gene            5750..6736
                     /gene="fliM"
                     /locus_tag="PKN91_01640"
     CDS             5750..6736
                     /gene="fliM"
                     /locus_tag="PKN91_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNS26801.1"
                     /translation="MSNHQILNQEEVDALLDGVDSGAVATEAKLPPGEARDYDFSSEM
                     RIVRGRMPTLEMINERFARLFRTSMYNMLRRSPDITVGPVQMKKFGEYVHTLHLPTSL
                     NLVRVAPLRGTGLIVLGPTLVFAMVDNFFGGNGRHAKIEGRDFTATESRVIHMLLKRV
                     FENLREAWANVAPIEIEYLSSEMNPHFANIVSPTEVVVVTSFHVDLEGGGGDLHVTLP
                     YSMIEPLRDVLDSGIQSDRVEQDERWLYTLREEVEGAEVTLTTVLGRAHLSLAELVNM
                     KPGDVIPCDFSGQATVFAEDVPIFRGGFGVSRGQLAVKVEQLVRLRNAKGTT"
     gene            6736..7074
                     /gene="fliN"
                     /locus_tag="PKN91_01645"
     CDS             6736..7074
                     /gene="fliN"
                     /locus_tag="PKN91_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNS26802.1"
                     /translation="MNATASQAAVPAAEFEKLNEDAGGAREVNLDVILDVPVTLSMEV
                     GRTRIPIRNLLQLNQGSVVELERAAGESLDVFVNGTLIAHGEVVVVNEKFGIRLTDVV
                     SPADRIRKLK"
     gene            7071..7508
                     /gene="fliO"
                     /locus_tag="PKN91_01650"
     CDS             7071..7508
                     /gene="fliO"
                     /locus_tag="PKN91_01650"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNS26803.1"
                     /translation="MSLAAQVSGQAAAGTPFAAPAAMAPATTSGLAGAGEVTLALLAV
                     LLVIFGCAWCVKRLRNFARPQGRVLAVVDDLAVGQRERVVLLACGGERLLVGVAAGQV
                     SLLRVVPSAAFSGAEDTGPAATPAAASAPDFRTVLRRSLGLAR"
     gene            7505..8239
                     /gene="fliP"
                     /locus_tag="PKN91_01655"
     CDS             7505..8239
                     /gene="fliP"
                     /locus_tag="PKN91_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650226.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNS26804.1"
                     /translation="MRAWLAILALAAPMAASAAVGLPAVTMENAPGGGQTWTLSLQVL
                     AFMTAITFLPAILLMMTSFTRIVIVLAILRQAIGAGQAPPNQVIVGLSLFLTLFVMAP
                     VFDRINVEALQPYMAGSLEMGEALTRAGGPLRQFMLDQTRESDIATFVRISGGQGFAS
                     PEDVPFSILVPSFVTSELKTAFLIGFLIFIPFVIIDLVVASVLMSMGMMMLSPVLISL
                     PFKLMLFVLVDGWALVMGTLAGSFFQ"
     gene            8236..8505
                     /gene="fliQ"
                     /locus_tag="PKN91_01660"
     CDS             8236..8505
                     /gene="fliQ"
                     /locus_tag="PKN91_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNS26805.1"
                     /translation="MSPEDVLAVGRHALEITLLLAAPPLLVALAVGLLVGIFQAATQI
                     NEMTLSFIPKLIAVALSVLVAGPWMLKLIVAYMRELYEGIPGMLG"
     gene            8515..9285
                     /gene="fliR"
                     /locus_tag="PKN91_01665"
     CDS             8515..9285
                     /gene="fliR"
                     /locus_tag="PKN91_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNS26806.1"
                     /translation="MLTFTAPDLMTLISTYLYPFVRVGACLMVAPVFGASYVPGRVRL
                     VLALALAMLVAPFVPVPQVELLSAGSALVIAQQILIGVALGFALQVVFDAVALAGQLL
                     ANSVGLSFAFNVDPLRGVSTPVVGQFYTLLVVLTFLALDGHLALLEMLTGGLRTMPVG
                     RTGLGADALWMLVLWTGQVIYGALAVALPGMTAMLVVNLGFGVMSRAAPTFNLFAVGF
                     PVTLAFGLVVLLLSLPSVQSGFLALAGDAFAILDRLQR"
     gene            9295..10365
                     /gene="flhB"
                     /locus_tag="PKN91_01670"
     CDS             9295..10365
                     /gene="flhB"
                     /locus_tag="PKN91_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNS26807.1"
                     /translation="MSEENAQERTERATPKRLEEARKKGQVPRSKELSTCVVLLAACA
                     GLLLLGDMVGQSLAAALRHGLDLSRAEVFDESALVASLHAMLFEVGMTCLPVFAIMAV
                     AALAAPMAIGGWNFSGEALAPDLKRLNPITGFGRIFSMRGVVELGKAFGKFALVALVA
                     AWLLWRSADELEALGSEPLQVAIGHAAQLVGVTMLALAGALLVIAAIDVPYQLWRHAQ
                     DMKMSREEVRRETKESDGSPEVKGRIRQMQQALASGRMMQDVPTADVVVVNPTHYAVA
                     LRYDDARSRAPVVVAKGTDLIAARIREIAAEHDVPIVEAPPLARALHRSVEIGNEIPV
                     ALYAAVAQILTYVYQLRAKSSR"
     gene            10390..12471
                     /gene="flhA"
                     /locus_tag="PKN91_01675"
     CDS             10390..12471
                     /gene="flhA"
                     /locus_tag="PKN91_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNS26808.1"
                     /translation="MAQSSKLALPSLHTLVRSGLGVPIGLFAILAMVVVPMPPLVLDV
                     LFTFNIALSIVIVMAVFYVARPLEFGVFPTVLLLATLLRLALNVASTRVVLMHGHQGT
                     GAAGHVIESFGEFVIGGNYVVGMVVFVILTIVNFVVVTKGAGRISEVSARFTLDAMPG
                     KQMAIDADLNAGLITQDEARRRRGEVRAEADFYGSMDGASKFVRGDATAGILILIINM
                     IGGLAIGTMMHDLPLGDAARTYSLLTIGDGLVAQLPALLLSTAVALIVTRMSGEQDMG
                     GEVARQLIGNPKALWIAAGLLTVMGVVPGMPNSAFLAMAGACAAVAWWRSRAREAAPQ
                     ADAVPAPPPSPESREITWDDVRPVDAISLEVGYRLVPLVDASRSGDLLARIRGVRRKL
                     SQDLGFLIQSVHIRDNLELSPNAYRINLAGVAVGEGVVHVDRELAINPGRVFGTLAGL
                     ATRDPAFGMEAVWIEPDKREHAQTLGYTVVDAGTVIATHLSHVVSQHAHELLGHEEAQ
                     QLINGLARTTPKLVEDLVPKVLPLSVVVRVLQGLLAERIPIRNMRAIAEALSEAALRT
                     QDPAALLAHVRIALGRQIVQDIAGPVEEVPVITIEPELEQLLQGSAQGAGNVTPGLEP
                     GLAERLQRQVAEAARRLEGAGEPAVLLTPPPLRATLARFLRAGIPGLHVLAWNEIPDS
                     RRVRLVTAIGR"
     gene            12497..13708
                     /gene="flhF"
                     /locus_tag="PKN91_01680"
     CDS             12497..13708
                     /gene="flhF"
                     /locus_tag="PKN91_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNS26809.1"
                     /translation="MNMRRYVARDMRECLRQIREAQGPDAVILSTRKVEGGVEVVAAI
                     DFDLAPADLAAAPAQALDTASLVTSTDEAEREATAALAALAGRSDPAAGPSTAEMQAE
                     LRSLRHLLESQLATLAWNDLTRRAPVQAEVLKELTQIGIANDIAASIIADLPPRLDLQ
                     RAQNRARAILTHRVQVVENGWLERGGRVAFVGPTGVGKSTAIAKLAAHWVLERGAQGI
                     ALVSTDGVRFGAQEQIQNLGRLLGVPAHVVDHPRELEQLLAGLADTPLVLIDTAGTSQ
                     RDQRLAAKLTGIAGAHPRLEMALVLSASSQAGAAEEVLARFAAAPLAACIVTKLDEAT
                     SLGGALSALIRARLPIAYVSDGQRIPEDLSPARAHQLIVRAVTLAERSGATADEDLLQ
                     RRFGGIAHVLA"
     gene            13728..14579
                     /locus_tag="PKN91_01685"
     CDS             13728..14579
                     /locus_tag="PKN91_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010449902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNS26810.1"
                     /translation="MRTRPGRPGPVQVIAVTGGKGGVGKTSVSVNLATALAAAGREVM
                     LFDGDLGLANVDVFLGLSPQYTMAHVLAGERTLDEVVLEAPQGFRVIPGASGIATLAN
                     LGAVEHLGLVQGFSALATGLDTLIVDTAAGISHGVLQFSQAAQQVLVILCDEPASLTD
                     AYALVKVLSRNHGVRGFRVLANMVRHRGDGEMMFRNLERVTGRFLDVTLEFAGEIPDD
                     PWMRRATRAQRTVLEAYPASAAAQAFKNLATAADNWPVPEAPRGNVEFFMERLVVNRG
                     PQLRVVQ"
     gene            14576..15298
                     /locus_tag="PKN91_01690"
     CDS             14576..15298
                     /locus_tag="PKN91_01690"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HNS26811.1"
                     /translation="MSSVRAYAARSGPRDADALVERHAELVKRIAYHLVGRLPASVEV
                     DDLIQAGMIGLLEAAANYVPDRGASFETYAGIRIRGAMLDALRKLDWAPRAVHRRARE
                     VAEAVRAVEAGIGREARDVDIAAWMQIPLEEYHAIVQDSALCQVASLDDATAAAVADQ
                     GMDPLRDTVDDEFRTALAAQIGELPEREKLVMSLYYDDELNLKEIGAVLGVTESRVCQ
                     LHGQALARLRARLAEWRDGQED"
     gene            15306..15701
                     /gene="cheY"
                     /locus_tag="PKN91_01695"
     CDS             15306..15701
                     /gene="cheY"
                     /locus_tag="PKN91_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HNS26812.1"
                     /translation="MRSKLKFLIVDDYSTMRRIVKNLLHDLGYPNVAEADDGRTALPM
                     LQAGGFDFVVTDWNMPDMPGLDLLKAIRADENLKELPVLMVTAEAKRDQIVEAAQAGV
                     SGYVIKPFTAETLKQKLDKILAPRAVAAK"
     gene            15705..16265
                     /locus_tag="PKN91_01700"
     CDS             15705..16265
                     /locus_tag="PKN91_01700"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HNS26813.1"
                     /translation="MSAVRPDTDRHAELLARLGNALARFQCEANPQCAATDAVPDARQ
                     RLAHVVRLTDDAAHRTLDLVEQSGPLAARIGQGARSLAVSAADGAGCEGEIAAYLAAT
                     AVDAETLRRNLSEVLLAQCYQDLTGQIIRGVINLVSEIADVLAELAHETGHTGSAHAP
                     ALAHAVDGSALKGQQDVDALFSDLGL"
     gene            16287..17030
                     /locus_tag="PKN91_01705"
     CDS             16287..17030
                     /locus_tag="PKN91_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HNS26814.1"
                     /translation="MDVLSLVGFLLAVIAVIVGSILKGAGIAALASSAAFMIVVVGTV
                     AAIFVQTSMPVLKHALRITGWVFRPPRLEAGAMIGKIVEWSNIARKQGLLGLEPMIEK
                     ESDPFLRKGLQLVVDGSEPDSLRGTMEVELSSREHADTAGAKVFEGMGIYSPTLGIIG
                     AVLGLMAVMQNLADPSKLGQGIAAAFTATIYGIGLANLVFLPMAAKLKSVISAQSQMR
                     EMIIEGLIGIAQGDNPRAIEARLQSFMHG"
     gene            17043..17942
                     /gene="motD"
                     /locus_tag="PKN91_01710"
     CDS             17043..17942
                     /gene="motD"
                     /locus_tag="PKN91_01710"
                     /inference="COORDINATES: protein motif:HMM:NF006541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HNS26815.1"
                     /translation="MSRRRRKHEEHSNHEAWAIPYGDLVTLLLAFFVVMYAISSVNEG
                     KYRILSDSLVAAFRGTPRTLEPIQVGEKQVGSGADLKVSLVEQGTLEGQPRQMLEAVP
                     LENAHPRRNGLRREGAAGQPLPGAKATDEAMLRKVADEVERAMQDLVERKQVLVRRYV
                     DRVEVEIRTDILFRSASAELSPHAQAIIGRLSDALIRFPNPVRVEGHTDNVPIRTRAY
                     PSNWELSAARAATVVHIMSQRGVEPARLAVLGYGEFRPARPNDSAEGRNMNRRVLLVV
                     TGVGENPEAAASPVAVAAQPVSP"
     gene            18048..18473
                     /locus_tag="PKN91_01715"
     CDS             18048..18473
                     /locus_tag="PKN91_01715"
                     /inference="COORDINATES: protein motif:HMM:NF022428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HNS26816.1"
                     /translation="MNELDLVAWVLAAAIVALGVAVAALAIGHARLREVSRRDAQRLF
                     EQMDLALGELREVSTSLSACLQELGGLRARSTPTQRAPEVAPAPTRSYDIALRLARNG
                     ASREELMSNCGMSRHEAELAVRLHGPGERRRPQRAGAAA"
     gene            18523..19797
                     /locus_tag="PKN91_01720"
     CDS             18523..19797
                     /locus_tag="PKN91_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNS26817.1"
                     /translation="MGVRLQKFHDAFIDECLESLDSMEAALLGLVVGAPDVEAVNRVF
                     RVAHSIKGASGMFGFTEIASFTHTLESLLGELRAGRMQVTSHILDLLLKSVDLLRDML
                     GAVQRKAPIDQQALVDLQFDLEQLIATSSAATGTAGAATGITGIYPARHIGGDVGSVR
                     VSTGKLDELVDAVEEIVATQSMLTGLGRLFQGAEAERLRVSLAQLERNVRGLRRGVMR
                     VRMLPISTIFGRFPRLVRDLSLRLGKQIQLQVTGDQIELDKAVLEKLADPLMHIVRNS
                     IDHGIEIPQVRTAAGKPPVGQVRIEVRHTDAMITVEVADDGGGLDHRRILEKARSTGL
                     VGACELPTVDAIHALIFLPGFTTAEQATELSGRGVGMDVVRSNITDLGGAVEVRSEAG
                     RGSRFIMTVPTRGAGYTAQYRALACSAGRAAG"
     gene            19859..20752
                     /locus_tag="PKN91_01725"
     CDS             19859..20752
                     /locus_tag="PKN91_01725"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNS26818.1"
                     /translation="MVNPSAQPATRTSAVHDVFAMALAQSLAQELASGRVELPGFPNV
                     ATQVQRVLADEDVTPLRVVRVIGAEPVLAGRVLQCANSVVFNPGGVPVSGLRAAVARV
                     GLDFVRTLTISFAVNQLKSAPTLRAIGPQLDALWLRSVKASTLCFVIARRLARTSADA
                     AFLAGLLHGVGALYIQTRAVDHPELQRRPDSYAMIERTWEAHIARALLEGWKVSEDIV
                     DAIGALHRVERGGDGPAGLREVLRAGVLITGMQASPQELRLMLASSDACAQLGLTPEV
                     YESLVRESAVEVAALRSVLGC"
     gene            20778..21659
                     /locus_tag="PKN91_01730"
     CDS             20778..21659
                     /locus_tag="PKN91_01730"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNS26819.1"
                     /translation="MTAANEKTEAAPKGAGAFEFVQDLARELSSGRIELPSFPDVAIR
                     VQRVLADEDVRPEAVVRIVGSEPALAAWVLRMSNSAAMSTGRQITDLRSAITRLGFNV
                     VRSAAVSFAMSQLTRAEDLKGLEEDLKLLWRRSTLVASMSNVVARRFSAVNPDTAMLA
                     GLLHGIGRLYILVRSRDFPTLFADASSYGQIVSDWHANVAKAILENWSIAEEIVDAVH
                     AYEQIYREHPDSADLTDVLITSDLLASFHEWPESLELNLQGVSAVDRLQLDRAGLERI
                     IVESREEISALRQALGG"
     gene            21680..22168
                     /locus_tag="PKN91_01735"
     CDS             21680..22168
                     /locus_tag="PKN91_01735"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNS26820.1"
                     /translation="MKILLVCLGNICRSPTAEGVLRALAQREAPQLALHADSAGTAGY
                     HIGAPPDPRTLAAARRRGYDLSDLRARQVQSADFSDFDLLLAMDEDNLASLRAQAPPG
                     TDQRLKMFLEYAPELGRLDVPDPYYGTAPDFDLVVDLVERGARGLLRALATHSSRSVR
                     SG"
     gene            complement(22138..24642)
                     /locus_tag="PKN91_01740"
     CDS             complement(22138..24642)
                     /locus_tag="PKN91_01740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNS26821.1"
                     /translation="MKRMLVNATQQEELRVALVDGQKLYDLSIELPSKEQKKANIYKG
                     RISRVEQSLEACFVDYGSERHGFLPLKEVSKEYFRKSPEGGRMNIRELLTEGQEVVVQ
                     VEKEERGNKGAALTTFISLAGRFLVLMPNNPRAGGVSRRIEGEDRDQMRQVMDALQVP
                     EGMGAIVRTAGVGRTVEELQWDLDNLRQQWEQIAAAIEGRPAPFLVYRESDPVSRALR
                     DYLGDDIGECLVDDPAAFANAQEYMQRSMPADAQRKLKLYTDDVPLFTRFQIESQIES
                     AYAHKVQLPSGGSLVIDYTEALVSIDINSARATRGSDIETTALNTNLEAADEIARQLR
                     IRDLGGLIVIDFIDMESQKSQREVEQRLRDAVRMDRARIQIGHLSRFGLLEMSRQRLR
                     PSLDESSHIACPRCSGIGSIRSVESMTLAILRLIGEEMRKDRTARVIVEVPVDVATYL
                     VNEKREWLRTLEDKSDVELVLVPNINIKTPEYAIRRLRHDEVDQPEFRRSSYKLPTPP
                     ALPDPATGQQKPLEPAAVPTFQPATAAPIVVAPVAAAAAPAIEPAAAQAPAGGFWARV
                     RQWLSGDEAAAAAPVAAAPAAPAHAARSRGQARRDGGQRDRGGRDRHRRGERGDRGDQ
                     ARQQRGEQRGGRDGSQQRARSGDGGGRRDGGQRPPREGQRDPQREAQRQRDRDAQRER
                     QARREREAPPADAAEAAAAAGAAATVVATEAQAGAEEMAGGPAATSEERGERRPRRRG
                     RRGRRGGRDRGEREASAAPAGDADTGGSGDGNGEGSSQSEANREFDFAPASPPEPTPP
                     PPPARTADPGAPQLVWSSRPTSVAPGSQPERTDRDE"
     gene            24956..25999
                     /locus_tag="PKN91_01745"
     CDS             24956..25999
                     /locus_tag="PKN91_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNS26822.1"
                     /translation="MVDASAPQPGTPRSAVRQVEATTEEAGLRLDKYVAKALPQVPLS
                     HVFRLIRKGEVRVNGKRASGSQRLQAGDIVRLPPVRAAVAPVDADGSPAPRRVPPRLI
                     EEIRGAVMHRDEQLLVVNKPAGLAVHGGSGVSFGVIEALRAAYPDDTLELVHRLDRDT
                     SGCLLVARKASTLRTLHALLREHEFEKRYLALVIGKWDLGKKRIDMPLRTDLRVGGER
                     TVKVQPSGKSAISDFRPVQFFGNRATLLEVSIRTGRTHQIRVHASYAGHPVAGDDKYG
                     DREANAMLKDFGLRRMFLHASSVSFTWPQSGTEFSANAPLPADLAAVIDNLAAAPVRG
                     RRSPRGARRPRAR"
     gene            complement(25950..26534)
                     /locus_tag="PKN91_01750"
     CDS             complement(25950..26534)
                     /locus_tag="PKN91_01750"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNS26823.1"
                     /translation="MFHRPLILASTSPYRRELLGRLALPFQVESPGVDELGMAGELPP
                     ARAARLARDKALAVARRHPSAVVIGSDQVAALDARLLEKPGNAERCRAQLDSLSGKGA
                     LFHTACAVIGLDAGIDLAHTDTTRVVARALTGEEIDRYIEREQPFDCAGGFKAESLGI
                     ALFDRIETTDPTALVGLPLIWLAGALRRAGYAVP"
     gene            26593..27096
                     /locus_tag="PKN91_01755"
     CDS             26593..27096
                     /locus_tag="PKN91_01755"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HNS26824.1"
                     /translation="MPDGWSRPLDAEQLADRRSDIEFSIPLPALPRIAPLLARPEGSA
                     RGKASFAREQSLAVVDLTVEAELSLVCQRCMQPMSWPVVGRAHIALVGDADAADAVAS
                     EFETVIAPGGRVTVGELVEEELLLALPVVALHARADACGVQLDAEPDKEVQKPFAGLA
                     DLLKRGP"
     gene            27113..27307
                     /gene="rpmF"
                     /locus_tag="PKN91_01760"
     CDS             27113..27307
                     /gene="rpmF"
                     /locus_tag="PKN91_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011147057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNS26825.1"
                     /translation="MPVQKSRRTPSTRGKRRVHDRLARPALSIDPQSGETHLRHRITP
                     DGFYRGKRVIEKPVESEGEE"
     gene            27359..28372
                     /gene="plsX"
                     /locus_tag="PKN91_01765"
     CDS             27359..28372
                     /gene="plsX"
                     /locus_tag="PKN91_01765"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNS26826.1"
                     /translation="MPTLAIDVMSGDLGATECVPGALLALARSPDLRVELVGSPEAIE
                     PLLGAADAALRARLAIVPAADVVPMDELPRAAIRHRKHSSMRIAVDRVKEGAAVGCVS
                     AGNTGALTAIAHFVLKTVPEVERAPIMSALPAAHGHTYMLDLGANISASAVQLCQFAM
                     MGSIVARDVYGVAEPRVGLLNIGEEDIKGHDTVQSANALIAASGVRYLGFIEGDDIFS
                     GEVDVVVTDGFTGNVALKSMEGLARLITDTMRAEFTRSALSRAAALVARPVLKRVGLA
                     LDPRRYNGACMVGLRGVVVKSHGRADRTAFARAIELTRTAALHDLPGCVARALGAQPP
                     QGS"
     gene            28372..29337
                     /locus_tag="PKN91_01770"
     CDS             28372..29337
                     /locus_tag="PKN91_01770"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNS26827.1"
                     /translation="MYSRIAGTGSYLPEKVVTNFDLAKVMDTTDEWIRERTGIVERHI
                     AAEGEATVDLAEKAARRAIEAAGIDPKEIDLICFGTTTPDLVFPNCGVLLQGRLGLRG
                     CASMGLEAACTGFLYSLSIADKFIRLGEAKCALVVGAETLSRITDWTDRGTAILFGDG
                     AGAVILKPDTEAGVVSTHLHADSTYKDLLYCPAGVSKGIGRPGDPPMHIHMSGNETFK
                     VAVNTLGRIVDETLAANSLDKSAIDWLVPHQANIRIIQATARKLDMPMERVIVTVDRH
                     GNTSAASVPLALDTGVRDGRIKRGELLLLEAFGGGFTWGSALIRY"
     gene            29340..31103
                     /locus_tag="PKN91_01775"
     CDS             29340..31103
                     /locus_tag="PKN91_01775"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HNS26828.1"
                     /translation="MTARTVALGLAFVQGIVLWWLTASARAKSWPATDSAMLAALLTP
                     AVFVPPLVYLQLGLAHLRTVALVSLAAGLVLGALGWHYGAWIAGAGLPTGGLFGEDAF
                     AFAIAVLVLCFHALPFLQSALVARRQWPAYPMLFEFAWRNALQIALAMLFCAALWALL
                     ALWMGLFRMIGIDFFREAFTDPRVAFPLTTVAFATALALSGPVERLQQALRQQLLALF
                     KWLAVLAMLILTLFSIALLLRSPELFGTGRRAISAAWLLWLLVFCVYLLNAAYQDGGH
                     AAPYPRPVGVALRVATPLLVVVAVLALYALGVRVGAYGLTVPRIWAMLAAALALVYAV
                     GYTIAATRRGAWMASMGAVNVVAAVMLIVALLLMLSPALSPYRLAADSQAARALRGAD
                     GSTAARGPHVRYPGSYADEYVTLAFDTGAYGRQWLARLARLEGHPDAARIRARAATML
                     ASRQRYDVAPALAANLPMTLVAFPEGREIDAGLRAAIEGAQVPRWLGPLRSSEVGSYP
                     VLFADLDQDGVEEVFVFSEPQAFMLTRKDGVWRETMLLGGGVSRTHALERLQAGDYRL
                     VAPRWQKLEVGGVTLTVMPDQ"
     gene            complement(31156..31467)
                     /locus_tag="PKN91_01780"
     CDS             complement(31156..31467)
                     /locus_tag="PKN91_01780"
                     /inference="COORDINATES: protein motif:HMM:NF023267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine-zipper protein"
                     /protein_id="HNS26829.1"
                     /translation="MKSAIVKVAAVAAVVMLAGCTDLKPLQAQVDDLKAQVGRLASDT
                     AAAKSAADAASSAAQSASSTASGAQSTANQALAAAQASQSCCDATNEKIDRMFKRSIS
                     K"
     gene            31803..32417
                     /gene="lexA"
                     /locus_tag="PKN91_01785"
     CDS             31803..32417
                     /gene="lexA"
                     /locus_tag="PKN91_01785"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNS26830.1"
                     /translation="MSDLTPRQTEILRLIQRTMAETGMPPTRAEIAAELGFRSANAAE
                     EHLRALARKGVIALIPGTSRGIQLKDTMREQLGLPLIGRVAAGRPILAEENIEARYQI
                     DPGIFQPKPHYLLKVTGMSMKDAGILDGDLVAVHRTPEVRNRQIVVARLENEVTVKRY
                     RQEGSIAWLLPENADFEPIRVDLKQEHMIIEGVVVGVLRRGKIS"
     gene            32414..32782
                     /locus_tag="PKN91_01790"
     CDS             32414..32782
                     /locus_tag="PKN91_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNS26831.1"
                     /translation="MSIQGLRTAAYHVGDLAAAKAWYSRVLEREPYFDQPFYVGYEVG
                     GFELGLVPDGKPGPGGTVVYWGVPDAAAELRRLESLGAAVRDPLQDVGEGILTATVTD
                     PFGNVFGVIENPHFSLAKVR"
     gene            32810..33439
                     /gene="imuA"
                     /locus_tag="PKN91_01795"
     CDS             32810..33439
                     /gene="imuA"
                     /locus_tag="PKN91_01795"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HNS26832.1"
                     /translation="MSDDPLARLLQHPALLRGRSAAQLPTWSTGFPSLDASLPGGGWP
                     RAGLVELLTPRPGVGELRLLVPVLAQLSGLTPARWLAWIAPPFEPFAPALAERGIALD
                     RQLIVRTAAPLWAMEQALGSGACEAALAWAPRVRARGLRRLQLATERGRTLGIVFREA
                     SAAREPSPAALRLAVVPHGGGVEVSLLKSRGGARGAIHLQFGDGRAEER"
     gene            33423..>34177
                     /locus_tag="PKN91_01800"
     CDS             33423..>34177
                     /locus_tag="PKN91_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HNS26833.1"
                     /translation="MPKSAEDLEFEAAGRGSPVGAAALRRPTPSPACSPAPAPAPALS
                     AEAGELWLALHLPTLAFDAVCREGDASQPVAVVEPVRRVQRIVIASRAAHARGVAPGM
                     ALAAALTLEPRLDTRNRDTGAERALLERLAAAAGEFTSRVSLEPPDGVLLEVRGSLAL
                     FGGVDALCAQVSAACRRVGTRARLALAPTPLAALAGARVSRGRAAAPFRVMHRAQLVG
                     ALAPLPLATLRLPQDTLGRLAKMGVNGIGAALRL"
BASE COUNT         5154 a        11546 c        11882 g         5595 t
ORIGIN      
        1 ggtgccgggc acggcgctgg cacccggggt tcagccgtcg ggcgcccaac cggccgcagc
       61 gacccctggc gggccggaca atctctcgcg ccagtcgacg cgcaactacg aaatcgaccg
      121 tacggtcgcc tacacacgcc agccggccgg tcggctgcaa cgcctgaccg tcgccgtgct
      181 ggtcgatgac ctgatcgtgc cacagaaaga cggcgcggtc gttcagaagc cgctcagcga
      241 agagcagctc gcgcgcatca cgctgctcgt gaaggacgcg gtcggcttcg acgcggcgcg
      301 tggcgacagc gtcaatgtcg tcaatgcctc ctttgcgacc ggcgccgacc agcccgccga
      361 catcgtcgag gaaggcatcc cgttgtggcg tcagccatgg ctgcaggaac tcgtcaagat
      421 cattgccggt gccgcgatcg tgatcgtgct gctgctggcg gtcgtgcgcc cgctggcgcg
      481 gggcctgctg ggtcccgtgc gattcgcgcc gggcacggcg cctgcgctgc ccgcggcggg
      541 gggcgaggcc gttgccacgg cggacgagga tacgccgcgc ggcgcgggcc aggtctccgc
      601 gatcgcctac gaacaacagc tcgcgcaggc gcgctcgctg gtgacacagg atccgaagcg
      661 cgtcgcgcgc gtcgtcaaga catgggtggg acaggatgaa tgagaaaggg ctgatcgcac
      721 gcagcagcac ggaccaggcg gcgatcctgc tgctgtcgct cggcgagcag gaggcggccg
      781 aggtcctgaa gcacatgagc gccaaggacg tgcagcgggt cggcacggcc atggccgcgc
      841 tcgatggcgt gacgcgcgag gaggtctcgc aggtgctcgg gcacttcacg acgctggtcg
      901 aaagccacac ctcgctcggc gtcggtgccg acgagtacct gcgcaaggtg ctgatctcgg
      961 cgctcggcga tgaacgcgcg accaacatca tggaccgcat cctcggcggc cgctcgagcc
     1021 gcgggctcga ggccatgaag tggatggacc cgcgatcggt ggccgagatg atgcgcttcg
     1081 agcatccgca gatcgtggcg atcgtgctcg cctacctcga tccggatcag gccgcgtcgg
     1141 tgctcgggca gctgcccgag atggcgcgtt ccgacatcct gatgcgcatc gcgatgctcg
     1201 acggcatcca gccctcggcg ctcaacgagc tcgacgagat catggagaag cagtttgcgg
     1261 gcaacagctc agccaaggcg tcggtgttcg gcggtcccaa ggtcgccgcg agcatcatca
     1321 acttcctcga ctcgggccag gaaaccgtga tcatggagcg catcaaccgc gccgacgaag
     1381 cgctcgcgga gaagatccag ggactcatct tcaccttcga cgatctcgtg tcgctcgacg
     1441 atcgcggcat gcaggagctg ctgcgccagg tgccgggcga tcgcctgctg ctggcgctca
     1501 aggcctgcga gccgggcgtc aaggacaagg tattccggaa catgtcgcag cgggcggcgg
     1561 agatgctgaa ggacgatctg gaagcgaagg gcccggtgcg cgtgagcgaa gtggaggccg
     1621 cgcagaagga agttctcgcc accgcgaagc gcttggcgga tgccggcacg atttcgctcg
     1681 gcggcaaggg tggagaggcg tatgtctgac ccggcgcgct gggagctgcc ggtcgtcgag
     1741 ggccgcgtcg cgggccggcg ggcgcggccc atcacggctg acgagctcga cgacatcgcg
     1801 cgcgcggcct gggacgaagg gcacgccaag gggcatgccg acggcctggc cgcggcagcg
     1861 gcggaagtgc agcgccggca cgccgccctc gacgcgcgca ttgcgcgcct cgacgagatc
     1921 gcgaccctgc tcgcgcgtcc gctggccgaa ctcgacgcgg aggtcgaatc acagcttgcg
     1981 cagcttgcgc tcaccgtggc gggccagctg gtgcggcggg agctgcgcat cgaccggacc
     2041 cagatcatcg gcattctgcg ccacgtcgtg tcgctgttgc cggccgccgc gcgtgacgtg
     2101 cgcgtgcacc tgcatcccga cgatgcggcg ctcgtgaacg agaaactcgc cacgcccgcc
     2161 agcgagcgtg cgtgggtcct cgtggaggat ccggtgatgg cgcgcggcgg ttgccgcgtg
     2221 acggccgaga attcgcagat cgacgcgcgg ctcgaatcgc gcctcaacgc gacgatcgtc
     2281 gaagtgctgg gcgaggaacg gctcggcgac gggcgcgaac agtcatgacc ctgctgcagg
     2341 aacgacagct gcgctggcgc gagggactcg cgcgttacca gcgacgggcc ttggcgctgc
     2401 cgccgccacc ggtcgagggc acactgacgc gcgtcgtcgg tctgacgctc gaggccgccg
     2461 gctgcgaagc ggccgtcggc gatcgctgcg aggtcctgtc gacgctcggc ggaaaggtcg
     2521 aggcggaggt cgtgggattc gccggcgaac ggctcttcct gatgcccacc gcggatgcac
     2581 atgggctctc gcccaatgcg cgggtcgtcc cgcgcaccgg ccgccagacg gtccgggtgg
     2641 gtgaacagtt gctcggacgc gtcatcgacg gcgccggcca gccactcgat ggcctcggcg
     2701 cgctgcattg cgacgcgccc gtcacgctga tgggcggcgc gccgatcaat ccgctggccc
     2761 gcgaaccgat caccgagccg ctcgacgtcg gcgtgcgcgc aatcaactcg ctgctgacgg
     2821 tgggtcgcgg ccagcgcatc ggcctctttg ccggaagcgg cgtgggcaag agcgtgctgc
     2881 tcggcatgat ggcgcgctac accgcggcgg acgtcatcgt cgtcggcctg atcggcgagc
     2941 gcggccgtga ggtgaaggaa ttcatcgagc gcatcctcgg cgaggagggg cggcggcgcg
     3001 cagtcgtggt ggcggtgccg gcggaccagt cgccgctcat gcgcctgcat ggtgcatggc
     3061 tcgcgacggc ggtcgccgag tacttccgcg accagggcaa gagcgtgctg ctgctgatgg
     3121 attcactgac gcgcttcgcg caggcacagc gcgagatcgg actcgcgatc ggcgaggcgc
     3181 cggcgaccaa gggctatcca ccctcggtgt tcgcgcgcct gccgcagctc gtcgagcgcg
     3241 ccggcaacgg tgcgcgactc ggcggctcga tcacggcgtt ctacacggta ctcaccgagg
     3301 gcgatgacca gaacgatccg atcgccgaca gcgcccgcgc gatcctcgac ggccacttcg
     3361 tgctgtcccg ccggctcgcc gaggccggtc agtatccggc catcgacatc gaggcctccg
     3421 tgagccgggc gatgcacgat atcgtcgcgg aatcccactt cgaggcggcg cggcgcttcc
     3481 gccacgcgct gtccacctac acgcagaacc gcgatctcat ctcgatcggc gcctatcgcg
     3541 cgggcagcga cccgcgcgtc gatgcggcga ttgcgctctg gccgcgcatc cagaccttcc
     3601 tgcgccagca catgcgcgag cgcgcagatc tcgcggcgag cctggcggcg ctgtccgaag
     3661 tgaccgccga gggagccgac gcgtgacgcg cgcccggcgc atggatccgg tggtgcgcgc
     3721 ggtcggccac gacgaacgcg agcatgccgc acgcctcgcc gagtgcggtc agcgcgtcgc
     3781 ggatgccgaa cagcgcctcg cggagctcga gaaataccag gccgactacg cgagcggcct
     3841 gcacagccgc accgccgccg ggatcagtgc caccgagatg cgcgattttc gcgccttcct
     3901 cgcgcgcctg agcgaagccg tgcgcacgca gcagcgcctg ctcgcacagg cgcgggccga
     3961 acacgaggcg gcccgcgagg cgtggcggcg cgcggcgcag cgcgcgaggt cgatcgacca
     4021 tgtcgtcgag cgctggcagg ccgaggagcg gatccatcgc gagcggcgcg agcagatcga
     4081 tatcgacgaa cgtgccctgc agctcacgcg cgcgcgcacg cgtcactgaa catggccaaa
     4141 ggacccgaca cgatgatgac gactgcaccg gtatcgcccc ccgccgcgag taccgcaccc
     4201 gcggagtcgc ccacgggagc cgatccggcc ctggccgggt tgttcggcga ggagctggcg
     4261 caactgctgc tcgaagagca gggcggtgac gacgacgaag cgctggcgcc ggaggcgctg
     4321 gcgctgctgc tcgctgcggt ggtcgaagca ccgcccgcgt cgtccgcggc gcccgcggca
     4381 gccgatgccg actcgccgat gggaggtgtc ctgaggcgac tcgagctgca tggtgcgcca
     4441 gctgcgcctg ttgattccgg ggccgaggcg tccctggacg gcatgttgca ggagctgcgc
     4501 acctcgaccg caggttcgcg cagccttgcg gagagcccgg cagcgctcac ggcacaggcc
     4561 ggctcgacga cggtgcagat tgcgaccacg tcggcgaccg accatctggc cgggctcgtt
     4621 gccgcgagtg gtcaggccgc ggattcgcgc cccacgacac agcacgccgc gctacgccac
     4681 gagctgccgc tgcaaacgcc ggtgggatcg catgcctggg gcgacgaact tgcctccagg
     4741 atcgccacga ttgcgaccga tggaccgcag tcggcgtccg tgcgcctctc gccggagcat
     4801 ctcgggcccc tccacatccg catcgcggtg cgcgatggcg acgcggccgt caccttcggc
     4861 gcaagccacg cagacacgcg cgcggcactc gagcagtcgc tcccgaggct gcgcgagctg
     4921 ctcgccgcgc aaggcctcaa tctcgcggag gcgagcgttt cggagcacgc cccacgcgag
     4981 gatcgcgcac cgactcccgc aggccgtcga gccgtcgctg cgggcgcaga tccggtgcag
     5041 gacgccgggc gcagggaggc acggcccgtc ggtctcgtgg acctgtacgc ctgagcaggc
     5101 ggtagcgtca aatccccgtc gcccctggca ggcgtgcgct gcttcacact ctccggctgg
     5161 atggaaatca ctgcacatca atgacttagg ccctggattg cagggctggc accagccttg
     5221 caacgttgct cgcgcgcgaa ctcgcgtcga ggaaccgaca tggtcgaagc tgctgtctcc
     5281 agcccaccgc ggaagtcgtc gaaggcgctt ctgtggatcg tactggtcct gctcgtgggt
     5341 gccgccggcg ccgcgggctc ctggttctac ctgggccgca gtgccgcgcc cgcagctgca
     5401 gcagtgcccc ggccgcagcc ggcactgttc catcagctgg atccctcgtt cgtcgtgaac
     5461 ttcgaggccg agcagatggt gcgcttcctg caggtgacga tcgagatcat gacgcgcgat
     5521 ccagccgccc tcgaattcgt gcggcagcat gagccggtgg tgcgcaacga cctgctgatg
     5581 ctgctttcca atcagaagta cgaggtgctc gccacgcagg ccggcaagga cgagctgcgc
     5641 gcgcgcgccc tcgaggccgt gcgcgcggtg gccgcgcgcg aggggggcga ccccgcgaag
     5701 ctcgaggccg tctatttcac gtccttcgtg atgcagtagg cgacctcgga tgtccaacca
     5761 ccagatcctg aaccaggaag aggtcgacgc gctgctggac ggcgtcgaca gcggcgcggt
     5821 cgcgaccgag gcgaagctgc cgcccggcga ggcacgcgac tacgacttca gcagcgagat
     5881 gcgcatcgtg cgcgggcgca tgcccacgct cgaaatgatc aacgagcgtt tcgcgcggct
     5941 gttccgcacc agcatgtaca acatgctgcg tcgctcgccc gacatcacgg tcgggccggt
     6001 gcagatgaag aaattcggcg agtacgtgca cacgctgcat ctgccgacga gcctcaacct
     6061 cgtgcgcgtg gcgccgctgc gtggcaccgg cctgatcgtg ctcggcccga cactcgtctt
     6121 tgcgatggtc gacaatttct tcggcggcaa cggccgccac gccaagatcg agggtcgcga
     6181 cttcaccgcg accgaatcgc gcgtgatcca catgctgctg aagcgcgtgt tcgagaatct
     6241 gcgcgaggcc tgggccaacg tcgcgccgat cgagatcgag tacctctcgt cagagatgaa
     6301 cccccacttc gcgaacatcg tcagccccac cgaggtggtg gtcgtgacgt cgttccacgt
     6361 cgacctcgag ggcggcggcg gggacctgca cgtgaccctg ccgtattcga tgatcgagcc
     6421 gctgcgcgac gtgctcgatt cgggtatcca gagcgatcgc gtcgagcagg acgagcgctg
     6481 gctgtacacg ctgcgcgaag aggtggaagg cgccgaagtg acgctgacca cggtgctggg
     6541 ccgcgcgcat ctgtccctcg ccgaactcgt gaacatgaag ccgggagacg tgatcccgtg
     6601 tgacttctcg ggtcaggcca cagtgtttgc cgaagacgtg ccgatctttc gcggcggctt
     6661 cggtgtctcg cgcggacagc tggccgtcaa ggtcgagcag ctcgtgcgcc tccgtaatgc
     6721 caaaggaacg acttgatgaa cgccactgcc agccaggccg ccgtaccggc ggccgagttc
     6781 gagaaactga acgaggacgc gggaggtgca cgcgaggtga acctcgacgt gatcctcgac
     6841 gtgcccgtga cgctgtcgat ggaagtgggg cgcacccgca tcccgatccg caacctgctg
     6901 cagttgaacc agggctcggt cgtcgagctc gagcgcgcgg ccggtgagtc gctcgacgtg
     6961 ttcgtgaacg gcacgctgat cgcgcacggc gaggtggtgg tggtgaacga gaagttcggc
     7021 atccgcctga ccgacgtcgt gagcccggcc gaccggatcc gcaagctcaa atgagcctgg
     7081 cggcacaagt tagcgggcag gccgccgcgg gtacgccgtt cgcggcaccc gccgccatgg
     7141 ctccggcgac gacctcgggc ctcgccggtg ccggcgaggt cacgcttgcg ttgctcgccg
     7201 tgctgctcgt gatcttcggc tgcgcctggt gcgtcaagcg cctgcgtaac ttcgcgcgac
     7261 cgcagggtcg cgtgctcgca gtcgtcgacg acctcgcggt cggacagagg gaacgtgtcg
     7321 tcctgctcgc ctgcggaggc gagcggctgc tggtcggtgt cgcggccggg caggtcagcc
     7381 tgctgcgggt ggtgccatcc gcagcgttct cgggggcaga ggacaccggc ccggcggcga
     7441 caccggcggc ggcatcggcg ccggactttc gcacagtcct gcgccgcagc ctgggactcg
     7501 cccgatgaga gcatggcttg cgattctggc gcttgccgcg cccatggcgg cctcggcggc
     7561 agtgggtctc cctgccgtga cgatggagaa cgcaccgggc ggcggccaga cctggaccct
     7621 gtcgctgcag gtgctcgcct tcatgacggc gatcaccttc ctgccggcca tcctgctgat
     7681 gatgacgtcg tttacgcgca tcgtgatcgt gcttgcgatc ctgcgccagg cgatcggtgc
     7741 cggccaggcg ccgcccaacc aggtgatcgt cggcctgtcg ctgttcctga ctctgttcgt
     7801 catggcgccg gtgttcgacc gcatcaatgt ggaggccctg cagccctaca tggccggctc
     7861 gctcgagatg ggcgaggcgc tcacgcgtgc gggcggtccg ctgcgccagt tcatgctcga
     7921 tcaaacgcgc gagagtgaca tcgcgacctt cgtgcgcatt tcgggcgggc agggctttgc
     7981 gagtcccgag gatgtgccgt tctccattct cgtgccctca ttcgtcacga gtgaactgaa
     8041 gacagccttc ctgatcggct tcctgatctt cattccattc gtgatcatcg acctcgtggt
     8101 ggcgagcgtg ctgatgtcga tgggcatgat gatgctctcg ccggtactga tctcgctgcc
     8161 cttcaagttg atgctgttcg tgcttgtcga cggctgggcg ctggtcatgg gcacgctcgc
     8221 cgggagcttc ttccagtgag cccggaggac gtgctcgcgg tcgggcgcca tgcgctcgag
     8281 atcacgctgc tgctcgccgc gccgccgctt ctcgtcgcac tggcggtcgg cctgctcgtc
     8341 ggcatctttc aggccgccac gcagatcaac gaaatgaccc tgtcgttcat cccgaagctg
     8401 atcgccgtgg cgctgtcggt gctggtggcg ggcccgtgga tgctgaagct gatcgtcgcc
     8461 tacatgcggg agctgtatga aggtatcccg gggatgctgg gctgaggcgg tccgatgctg
     8521 acatttaccg cgcccgacct gatgacgctg atctcgacgt atctttatcc gttcgtgcgc
     8581 gtcggcgcct gcctgatggt cgcgccggtg ttcggcgcga gctacgtgcc ggggcgcgtt
     8641 cgcctcgtgc tcgcgctcgc gctcgccatg ctggtggcgc cgttcgtgcc tgtgccccag
     8701 gtcgagctgc tgtcggccgg gagcgcgctg gtcatcgccc agcagattct gatcggggtg
     8761 gcactcggat tcgcgctgca ggtcgtgttc gacgccgtgg cgctcgccgg gcagctgctc
     8821 gccaacagcg taggcctctc gttcgctttc aacgtcgatc cgctgcgcgg cgtcagcacg
     8881 cccgtcgtcg gccagttcta caccctgctc gtcgtgctga ctttcctcgc gctcgacggc
     8941 cacctggcgt tgctcgagat gctgaccggc ggcctgcgca cgatgccggt tggccgcacg
     9001 ggcctcggcg cggacgccct gtggatgctc gtgctgtgga ccgggcaggt gatctacggt
     9061 gcactcgccg tcgcgctgcc cggcatgacc gcgatgctgg tggtcaacct cggcttcggc
     9121 gtcatgagcc gcgcggcgcc caccttcaac ctgttcgcgg tcggctttcc ggtcacgctc
     9181 gcattcgggc tggtcgtgct gctgctcagt cttccgtccg tgcagtcggg cttcctggcg
     9241 ctcgccgggg acgccttcgc catcctcgac aggctgcagc ggtagggcgc cggcatgtcg
     9301 gaggagaacg cccaggaacg taccgaacgc gccacgccca agcgcctcga ggaggcgcgc
     9361 aagaagggcc aggtgccgcg ctccaaagaa ctgtcgacct gcgtcgtgct gctcgccgcc
     9421 tgcgccggcc tgctgctgct cggcgatatg gtgggacagt cgctggcggc ggcgctgcgc
     9481 cacgggctcg acctcagccg cgcggaggtc ttcgacgagt ccgcgctcgt cgcttcgctc
     9541 cacgcgatgc tgttcgaagt ggggatgacc tgcctgccgg tatttgccat catggccgtg
     9601 gccgcgctgg ctgcccccat ggccatcggc ggctggaact tctccggcga ggccctcgca
     9661 ccggatctca agcgcctcaa cccgattacc ggcttcgggc gcatcttctc gatgcgcggc
     9721 gtcgtcgagc tcggcaaggc cttcggcaaa ttcgcgctcg ttgcgctcgt cgccgcatgg
     9781 ctgctgtggc gcagcgccga cgagctcgag gcgctgggct cggaacccct gcaggtggct
     9841 atcggccatg ccgcgcaact cgtcggcgtg accatgctgg cgctcgccgg cgcgctgctc
     9901 gtgatcgcgg ccatcgatgt gccctaccag ctctggcggc atgcgcagga catgaagatg
     9961 agccgcgagg aagtccgccg ggaaacgaag gaatcggatg gctcgccgga ggtcaagggc
    10021 cgcatccgcc agatgcagca ggcgctggcg agcgggcgga tgatgcagga cgtgcccacg
    10081 gccgacgtcg tggtcgtgaa ccctacgcac tatgccgtcg ccctgcgtta cgacgacgcc
    10141 cgctcgcgcg caccggtcgt cgtggccaag ggcacggacc tgatcgcggc ccgcatccgc
    10201 gagatcgccg ccgagcacga cgtacccatc gtcgaggcac caccgctcgc gcgtgccctg
    10261 caccgcagcg tggagatcgg caacgagatc ccggtcgcgc tgtacgccgc ggtcgcgcag
    10321 atcctcacct atgtctacca gctgcgcgcg aagtccagcc ggtaaccgaa ccgacgcaac
    10381 aggtaccaca tggcccagtc ctccaagctt gcactgcctt cgctgcacac gctggtccgc
    10441 tccggcctcg gcgtaccgat cggcctgttc gccatcctgg cgatggtggt cgtgccgatg
    10501 ccgccgctcg tgctcgatgt gctgttcacg ttcaacatcg cgctgtcgat cgtgatcgtg
    10561 atggccgtgt tctacgtcgc ccgtccgctc gaattcgggg tgtttccgac cgtgctgctg
    10621 ctcgcgacac tgctgcgcct cgcgctcaac gtcgcctcga cccgcgtcgt gctgatgcac
    10681 ggtcaccagg gcacgggggc cgccggccac gtgatcgagt ccttcggcga attcgtgatc
    10741 ggcggcaact acgtcgtcgg catggtggtg ttcgtgatcc tgacgatcgt gaacttcgtg
    10801 gtcgtcacca agggtgcggg acgcatctcc gaagtcagcg cgcgcttcac gctcgatgcc
    10861 atgccgggca agcagatggc gatcgacgcg gacctcaacg cgggcctgat cacgcaggac
    10921 gaggcgcgca ggcgtcgcgg cgaagtgcgc gccgaggcgg atttctacgg ttcgatggac
    10981 ggcgccagca agttcgtgcg cggcgacgcc acggccggca tcctgatcct gatcatcaac
    11041 atgatcggcg ggctcgcgat cggcaccatg atgcacgacc tgccgctcgg agatgccgcg
    11101 cgcacctatt cgctgctgac gatcggcgat ggcctcgtcg cgcaactgcc ggcactgctg
    11161 ctgtcaacgg cggtcgcgct gatcgtgacg cgcatgtcgg gcgaacagga catgggtggc
    11221 gaggtggcgc gccagctcat cggcaatccg aaagcgctgt ggatcgccgc gggcttgctg
    11281 actgtcatgg gtgtcgtgcc cgggatgccg aatagcgcct tcctcgccat ggccggggca
    11341 tgtgccgccg tggcgtggtg gcgcagtcgt gcccgcgagg ccgcacccca ggcggacgcg
    11401 gtaccggcgc cgccgccgtc gccggagagc cgcgagatca cctgggatga cgtgcgcccg
    11461 gtagatgcca tttcgctcga agtcggctac cgcctcgtcc cgctggtcga tgccagccgc
    11521 tccggcgacc tgcttgcgag gatccgcggc gtgcgccgca agctctcgca ggacctcggc
    11581 ttcctgatcc agtcggtgca catccgcgac aatctcgagc tgtcgccgaa cgcttaccgc
    11641 atcaatctcg ccggcgtcgc ggtcggcgag ggcgtggtgc acgtcgatcg cgaactcgca
    11701 atcaatcccg gtcgcgtgtt cggcacgctg gccggccttg cgacccgtga cccggctttc
    11761 ggcatggagg cggtgtggat cgagcccgac aagcgcgagc acgcgcagac cctgggctac
    11821 accgtcgtcg acgccggcac ggtgatcgcg acgcatctct cgcacgtcgt gagccagcac
    11881 gcccacgaat tgctcggcca tgaggaagcg cagcagctga tcaacgggct cgccaggacg
    11941 acgcccaagc tcgtcgagga tctcgtgccg aaggtgctgc cgctcagcgt ggtcgtgcgg
    12001 gtcctgcagg gcctgctcgc ggagcggatt ccgatccgca acatgcgcgc gattgccgag
    12061 gccctgtccg aagccgcgtt gcgcacccag gatccggccg cgctgctcgc gcacgtgcgg
    12121 atcgcgctcg ggcgccagat cgtgcaggac atcgcgggcc ccgtcgaaga agtgccggtg
    12181 atcacgatcg agccggaact ggaacagctc ttgcagggtt cagcgcaggg cgccggcaac
    12241 gtgacgcccg gcctcgagcc cggtctcgcc gaacgcctgc agcgccaggt cgccgaggcg
    12301 gccagacggc tcgagggagc gggggagccc gccgtgctgc tgacgccgcc gccactgcgc
    12361 gcgacgctcg cccggttcct gcgcgccggc attccgggcc tgcacgtgct cgcctggaac
    12421 gagattcccg acagccgccg cgtgcggctc gtcacggcca tcgggcgctg agtgaattgc
    12481 tgcaaagaga acgaacatga acatgagacg ttacgtggcg agagatatgc gcgaatgcct
    12541 gcgccagatc cgcgaggcgc agggccccga cgccgtcatc ctctcgaccc gcaaggtcga
    12601 gggcggggtc gaggtggtcg cggcgatcga tttcgatctc gcgccggccg atcttgcagc
    12661 cgctccggcg caggcgctcg acaccgccag tctcgtcacc tcgaccgatg aggccgaacg
    12721 cgaggctacc gctgccctgg ccgcgctggc tggccgcagc gatccggcag ccggacccag
    12781 tactgccgaa atgcaggccg aactgcgcag cctgcggcac ctgctcgaga gccagctcgc
    12841 aacactcgca tggaatgacc tgacgcgtcg cgcgccggtg caggcggagg tgctgaagga
    12901 gctcacgcag atcggcatcg ccaacgatat tgcggcctcg atcatcgccg atctgccgcc
    12961 gcgccttgac ctgcagcgtg cgcagaaccg cgcgcgggcc atcctcacgc acagggtgca
    13021 ggtggtggag aacggctggc tcgaacgcgg cggtcgcgtc gccttcgtcg gtccgaccgg
    13081 cgtcggcaag tccaccgcca tcgccaagct cgcggcacac tgggtgctcg agcgcggcgc
    13141 gcagggtatc gccctcgtgt ccaccgacgg cgtgcgcttc ggtgcgcagg aacagatcca
    13201 gaatctcggc cgcctgctgg gcgtgccggc ccatgtcgtc gatcatccgc gcgaactcga
    13261 gcagctgctg gcggggctcg ccgacacccc actcgtgctg atcgataccg cgggcaccag
    13321 ccagcgggac cagcgtctcg cggccaagct caccggcatc gccggcgcgc atccgcgcct
    13381 ggaaatggca ctggtgctgt ccgcgagctc gcaggcaggc gcggccgaag aggtgctcgc
    13441 gcgcttcgcc gccgcaccgc tcgccgcctg catcgtcacg aagctcgacg aggcgaccag
    13501 tctcggcggc gcgctgtcgg cgctgatccg cgcccggctg ccgatcgcct acgtgagcga
    13561 cgggcagcgc atcccggagg acttgtcacc cgcgcgcgca caccagctca tcgttcgcgc
    13621 cgtcacgctc gcggagcgct ccggcgcgac cgccgacgag gatctgctgc agcgccgatt
    13681 cggaggcatc gcgcatgtcc tcgcgtaatc cgcgcggtgc gcgcgccatg cgtacgcgtc
    13741 cgggccggcc gggcccggtg caggtgatcg cggtcaccgg tggcaagggc ggtgtcggca
    13801 agaccagtgt ctcggtgaat cttgcgaccg cgctcgccgc ggccggccgc gaggtgatgc
    13861 tgttcgacgg cgacctcgga ctcgccaatg tggacgtctt cctcggcctc tcgccccagt
    13921 acacgatggc gcacgtgctt gcgggcgagc gcaccctcga tgaggtggtg ctcgaggcgc
    13981 cgcagggctt tcgggtgatt ccgggggctt cgggcatcgc gaccctcgcc aacctcgggg
    14041 cggtcgagca cctcgggctg gtgcagggtt tcagtgcgct cgcgaccggc ctcgacacct
    14101 tgatcgtcga taccgcggcc ggtatctcgc acggcgtact gcagttttcg caggccgcgc
    14161 agcaggtgct ggtcatcctg tgcgatgagc ccgcctcgct gacggatgct tatgcgctcg
    14221 tcaaggtcct gagtcgcaat cacggcgtgc gcggttttcg cgtgctcgcc aacatggtcc
    14281 gccaccgcgg cgacggcgag atgatgttcc gcaacctcga gcgcgtgacc gggcgctttc
    14341 tcgacgtgac gctcgaattc gctggcgaaa tacccgacga cccctggatg cggcgcgcca
    14401 cccgtgcaca gcgcacggtt ctggaagctt accccgccag tgccgctgcc caggcattca
    14461 agaatctggc aaccgcggcc gataactggc ctgtaccgga ggcgccgcgg ggcaacgtcg
    14521 agttcttcat ggagcgcctg gtggtcaacc gcggtccgca gctgcgggta gtgcagtgag
    14581 cagcgtgcgc gcctacgcag cccgatcggg gccccgcgac gcagacgcgc tggtcgagcg
    14641 gcatgccgag ctggtgaagc gcatcgcgta ccacctggtg ggacgcctgc cggcgtcggt
    14701 ggaggtggat gacctgatcc aggccggcat gatcggcctg ctggaggccg cggcgaacta
    14761 cgtgccggac cgcggcgcga gtttcgagac gtatgcgggc atccgcatac gcggcgcgat
    14821 gctcgatgcg ctgcgcaagc tcgactgggc gccgcgtgcc gtgcatcggc gcgcgcgcga
    14881 agtcgccgag gcggtgcggg ccgtcgaggc cggtatcggg cgcgaggcgc gcgacgtgga
    14941 catcgcggcg tggatgcaga tcccgctcga ggagtaccac gcgatcgtgc aggacagtgc
    15001 gctctgccag gtcgcgagcc tggatgacgc gacggcggcc gcggtggcgg accagggcat
    15061 ggatcccctc cgcgacacgg tggacgacga atttcgcaca gcgctcgccg cgcagatcgg
    15121 cgagctgccg gagagagaga aactggtgat gtcgctctac tacgacgacg aactgaacct
    15181 gaaggagatc ggcgcggtgc tcggcgtgac cgagtcgcga gtctgccagc tgcacggtca
    15241 ggcccttgcg cgcctgcgcg cgcggctcgc cgagtggcgg gacggacagg aggattgagg
    15301 cggcgatgcg cagcaagctg aagttcctga tcgtcgacga ctactccacg atgcggcgca
    15361 tcgtgaagaa cctgctgcac gatcttggtt acccgaacgt cgcggaagcc gatgacggcc
    15421 gcaccgcgct gccgatgctg caggccggcg gcttcgactt cgtcgtgacc gactggaaca
    15481 tgcccgacat gcccggcctc gacctgctca aggcgatcag ggcggacgag aacctgaagg
    15541 agttgccggt gctgatggtg acggccgagg ccaagcgcga ccagatcgtc gaggcagcgc
    15601 aggccggcgt gagcggctac gtcattaagc ctttcacggc cgaaacactc aagcagaagc
    15661 tcgacaagat cctggcgccc cgcgccgtgg cggcgaagta gccgatgtct gcggtgcgcc
    15721 ccgacacgga tcgtcatgcc gagctgctcg cgcgcctggg caacgcactt gcacgcttcc
    15781 agtgcgaggc gaatcctcaa tgcgccgcga ccgatgcggt gcccgacgcg cgccagcgcc
    15841 tcgcacatgt cgtgcgtctc acagacgatg cggcccatcg cacgctcgat ctggtcgagc
    15901 agtccgggcc gctcgccgcg cgcatcggcc agggtgcacg gtcgctggct gtgagcgcgg
    15961 ccgatggcgc cggctgcgag ggagaaattg ccgcctacct cgcggccacg gcggtggatg
    16021 ccgaaacgtt gaggcgcaac ctgtcggagg tactgctcgc gcagtgctac caagacctca
    16081 ccggccagat catccgcggt gtcatcaatc tggtcagtga aatcgccgat gtgctcgcgg
    16141 aactggcgca cgagaccggg cacaccggca gcgcacacgc gccagcgctg gcgcacgcgg
    16201 tcgacggcag tgcgctcaag ggccagcagg acgtcgatgc cctgttttcg gatcttggac
    16261 tgtagcggcg gagcggcgcg aacacgatgg atgtactctc cctggtcggt ttcctgctcg
    16321 cggtcatcgc ggtgatcgtc ggttccatcc tcaagggcgc cggcatcgcg gcgctcgcct
    16381 cgtcggcggc cttcatgatt gtggtggtcg gcacggtggc cgcgatattc gtgcagacct
    16441 ctatgccggt gctgaagcac gcgctgcgca tcacgggctg ggtgttccgt ccgccccgat
    16501 tggaggcggg tgcgatgatc ggcaagatcg tcgagtggag caacatcgcg cgcaagcagg
    16561 gcctgctcgg gctcgagccg atgatagaga aggaaagtga tcccttcctg cgcaagggcc
    16621 tgcagctggt cgtcgacggc agcgagcccg attccctgcg cggcacgatg gaggtggaac
    16681 tgtcctcgcg cgaacacgcg gacacggccg gcgcgaaggt gttcgagggc atgggcatct
    16741 actcgccgac actcggcatc atcggcgcgg tactcggcct gatggcggtg atgcagaatc
    16801 tggccgatcc gagcaagctc ggccagggca tcgccgcggc gttcaccgcc acgatctacg
    16861 gcatcggact cgcgaatctg gttttcctgc cgatggcggc caagctgaag agcgtgatca
    16921 gcgcgcaatc gcagatgcgc gagatgatca tcgagggcct gatcggcatc gcgcagggtg
    16981 ataacccgcg cgcgatcgag gcccggttgc agagcttcat gcacggctga gtggcgcatt
    17041 ccatgtcacg caggcgtcgc aagcacgagg agcactccaa ccacgaggcg tgggccatcc
    17101 cgtatggcga cctcgtgacc ctgctgctcg cgttcttcgt cgtcatgtac gcgatctcgt
    17161 cggtcaacga gggcaagtac cgcatcctgt ccgattcgct ggtggccgcc ttcaggggta
    17221 cgccgcgcac gctcgagccg atccaggtgg gcgagaagca ggtcggctcc ggcgcggacc
    17281 tgaaggtttc gctggtcgag cagggcaccc tggagggcca gccgcggcag atgctggagg
    17341 ccgtaccgct cgagaatgcg cacccgcggc gcaatggcct gcgccgcgag ggagcggcgg
    17401 gccagccgct gcccggcgcg aaggcgaccg atgaggcgat gctgcgcaag gtggccgacg
    17461 aggtcgagcg ggcgatgcag gatctggtcg agcgcaagca ggtgctggtg cgtcgctacg
    17521 tcgatcgcgt cgaggtcgag atccggaccg acatcctgtt tcgcagcgca tcggcggagt
    17581 tgtcgccgca tgcccaggcg atcatcgggc ggctctcgga tgccctcatc cgcttcccga
    17641 atcccgtgcg cgtcgaaggc cacaccgaca acgtgccgat ccgtacgcgc gcctatccgt
    17701 cgaactggga actgtctgcc gcgcgcgcgg cgacggtcgt gcacatcatg agccagcggg
    17761 gcgtcgaacc ggcccgcctc gcggtgctcg gctacggcga gttccggccg gccaggccga
    17821 acgatagcgc ggagggtcgc aacatgaacc gtcgcgtgct gctggtcgtg accggcgtcg
    17881 gcgagaatcc ggaagctgcc gcctcaccgg tcgccgtcgc cgcgcaaccc gtcagtccgt
    17941 agaaagccac ttcctccgct gatggcatgc ttcctgcaat gtttctgcag acacttgcgg
    18001 gcgccggtga actgacgccg gcccggagtg cgaagggagt atccggcatg aacgaactcg
    18061 atctggtggc ctgggtgttg gccgcggcca ttgtcgcgct cggcgtcgcg gtggcggcgc
    18121 tggcgatcgg ccatgcgcgc ctgcgcgagg tttcccgccg cgacgcgcag cgacttttcg
    18181 aacagatgga cctggccctg ggtgagctgc gcgaggtctc gacatcgctt tcggcctgcc
    18241 tgcaggagct gggcgggttg cgtgcgcgca gcaccccgac gcagcgcgcc cctgaggtcg
    18301 cgccagcgcc gacgcgcagc tacgacatcg cgctccgtct tgcgcgcaat ggcgcgagtc
    18361 gcgaggagct gatgtcgaac tgcggcatga gtcggcacga ggccgagctc gccgtccgtc
    18421 tgcacgggcc cggcgagcgc cggcgtccgc aacgtgcggg ggccgccgca tagtgtgacg
    18481 cagttcactc aaggcgcccg cctgccgggt cgatatcggg gcatgggcgt caggctgcag
    18541 aagtttcacg atgccttcat cgatgagtgc ctcgagtcgc tcgactcgat ggaggccgcg
    18601 ctgctcggac tcgtggtcgg cgcaccggac gtcgaggccg tcaaccgggt gttccgcgtt
    18661 gctcactcca tcaagggcgc ctcgggcatg ttcggcttca ccgagatcgc ctcgttcacc
    18721 catacgctcg agtcgctgct cggcgaactg cgcgcgggcc gtatgcaggt cacgagccac
    18781 atactcgacc tgttgctgaa atccgtcgac ctgttgcgcg acatgctggg cgccgtgcaa
    18841 cgcaaggcac cgatcgacca gcaggcgctc gtcgatctgc agttcgatct cgagcagctg
    18901 atcgccacga gcagcgcggc gaccggtact gctggagccg ccaccggcat caccgggatc
    18961 taccccgcga ggcacatcgg cggcgatgtc ggttcggtac gcgtcagcac cggcaagctc
    19021 gatgaactcg tcgatgcggt cgaggagatc gtggcgacgc aatcgatgct gaccggcctg
    19081 ggtcggctgt tccagggtgc ggaggccgag cgcctgcgcg tcagtctggc gcagctcgag
    19141 cggaatgtgc gcgggctgcg gcggggtgtc atgcgggtgc gcatgctgcc gatcagcacg
    19201 atattcggcc gcttcccgcg actggtccgc gacctctcgc tgcgcctcgg caagcagatc
    19261 cagctgcagg tcaccggcga ccagatcgaa ctcgacaagg cggtcctcga gaagctcgcc
    19321 gacccactga tgcacatcgt caggaacagc atcgaccacg gcatcgagat accgcaggtg
    19381 cgcaccgcgg caggcaagcc tccggtcggg caggtgcgta tcgaggtccg ccacacggat
    19441 gccatgatta ccgtggaggt cgcggacgac ggcggtggcc tggaccaccg gcgcatcctc
    19501 gagaaggcgc gcagtaccgg cctcgtcggt gcgtgtgaac tgccgacggt cgatgccatc
    19561 cacgcgctga ttttcctgcc cggtttcacg accgcggagc aggcgaccga attgtcgggc
    19621 cgcggcgtcg gcatggacgt ggtacgcagc aacatcaccg acctcggcgg tgcggtcgaa
    19681 gtgcgctcgg aagcgggcag gggctcgcgc ttcatcatga cggttccgac ccgcggcgcc
    19741 ggctacacgg cgcaataccg cgcgctcgca tgcagcgcag gccgcgctgc gggctaaagc
    19801 ccgggccgct tgtgccgata acaggctggc cgaatgccgg cccctgacag gattcgaaat
    19861 ggtgaaccca tcggcccagc ctgccacccg aacgagcgcc gtgcacgacg tgttcgcgat
    19921 ggccctcgcg cagtcgctgg cgcaggaact ggcgtcgggc cgggtcgaac tgcccgggtt
    19981 tcccaacgtg gcgacacagg tacagcgagt gctcgccgac gaggatgtca cgccgctgcg
    20041 cgtcgtgcgg gtcatagggg ccgagccggt ccttgccggt cgcgtcctgc agtgtgcgaa
    20101 ctccgtggtc ttcaaccccg ggggcgtgcc tgtctccggg ctgcgtgccg cggtggcccg
    20161 cgtgggcctc gacttcgtgc gtacgctgac catttcgttc gcggtcaacc agctgaagtc
    20221 cgcgccgacc ctgcgcgcga tcgggccgca actcgatgcg ctgtggctgc gcagcgtgaa
    20281 ggcatcgaca ctgtgcttcg tcatcgcgcg ccgcctggcg cgcaccagtg ccgatgcggc
    20341 attccttgcc ggcctgctgc acggagtcgg tgcactctat atccagacgc gcgcggtcga
    20401 tcaccccgaa ttgcagcggc gccccgacag ctacgcgatg atcgagcgta cctgggaggc
    20461 gcatatcgcg cgggcgctgc tcgagggctg gaaggtgtcg gaagacatcg tggacgcgat
    20521 cggcgcgctg catcgcgttg agcgtggcgg cgatggtccg gcgggactgc gcgaggtgct
    20581 gcgcgccggg gtcctcatca ccgggatgca ggcctcgccg caggaactac ggctcatgct
    20641 ggcgtcgagc gatgcctgcg cccagctcgg gctcacgccc gaagtgtatg aatcattggt
    20701 acgtgagtcc gcagtcgagg tcgctgcact gcgcagcgtg ctcggatgct gatcggtcga
    20761 gagtcgagga gagagaattg actgctgcaa acgagaaaac ggaggctgcg ccgaagggcg
    20821 ccggggcatt cgagttcgtg caggacctgg cgcgcgagtt gtcgagcggc cgcatcgaac
    20881 tgccgagctt tccggacgtg gcgatccgcg tgcagcgcgt gctcgccgac gaggacgtgc
    20941 gacccgaagc ggtcgtgcgc atcgtcggtt cggaaccggc cctcgccgcg tgggtgctgc
    21001 ggatgtcgaa ctccgcggcg atgtccacgg ggcggcagat cacggatctg cgttcggcga
    21061 tcacccgtct cggcttcaac gtggttcgaa gtgcggccgt gtcgttcgcg atgtcgcagt
    21121 tgacgcgggc ggaggacctg aagggtctcg aggaagacct caagctgctg tggcggcgca
    21181 gtaccctggt cgcctcgatg agcaacgtgg ttgcgcgccg cttcagcgca gtcaatccgg
    21241 ataccgcgat gctggccgga ctgctgcacg gtatcggcag gctctacatc ctggtccgct
    21301 cgcgggactt cccgacattg ttcgccgatg cgtcgagcta cgggcagatc gtcagcgact
    21361 ggcatgccaa tgtcgccaag gcgatcctcg agaactggag catcgccgag gagatcgtcg
    21421 acgccgtgca cgcctacgag cagatctacc gcgagcatcc cgactcggcc gacctcaccg
    21481 acgtgctcat cacgtcggat ctgctcgcgt cgttccacga atggcccgaa tcgctcgaac
    21541 tgaacctgca gggcgtgagc gcagtcgacc ggctgcagct cgatcgcgcc ggactcgagc
    21601 gcatcatcgt cgaatcgcgc gaggaaataa gcgcattgcg ccaggcgctc ggcggctgat
    21661 ctgcgctatc gttgcgcagg tgaaaatcct gctcgtgtgc ctcggaaaca tctgccgctc
    21721 gccgaccgcg gaaggcgtgc tgcgtgcgct cgcgcagcgc gaagcgccac agctcgcatt
    21781 gcatgccgat tcggccggca cggcgggcta ccacatcggc gcgccgccgg atccgcgcac
    21841 gctggctgcc gcgcggcggc gcggatatga cctttcggac ctgcgggcgc ggcaggtgca
    21901 atccgccgac ttcagcgact tcgacctgct gctggcgatg gacgaggaca acctcgcgag
    21961 cctgcgtgca caggctccgc caggcacgga tcagcgtttg aagatgttcc tcgagtacgc
    22021 cccggaactc ggtcggctcg atgtcccgga tccctactac ggcaccgcgc ccgacttcga
    22081 cctcgtcgtg gatctcgtcg agcgcggtgc gcgtggcctg ctcagggcgc ttgcgactca
    22141 ctcatcgcga tcggtgcgct ccggctgact gcccggtgcg acgctcgtcg gccgggacga
    22201 ccagacgagt tgcggtgcgc cgggatcggc agtgcgtgcc ggcggtggcg gtggcgtcgg
    22261 ttcgggcggg ctcgcaggcg cgaagtcgaa ctcgcgattg gcttcactct ggctcgaacc
    22321 ctcgccgttg ccatcgccgc tgccgcccgt atcggcgtcg ccggctggcg ccgccgacgc
    22381 ctcgcgctcg ccacgatcgc gaccgccgcg tcgcccgcga cgcccgcgac ggcgcggccg
    22441 ccgctcgccg cgctcttcgc tggttgcggc gggaccaccc gccatctcct ccgcaccggc
    22501 ctgagcttcg gttgcgacaa ccgttgcggc tgcgccggct gcggccgcgg cttccgcagc
    22561 atccgccggc ggcgcctcgc gctcgcgccg ggcctgccgc tcacgctgtg catcgcgatc
    22621 gcgctggcgt tgcgcctcgc gctgcggatc acgctgccct tcgcggggcg ggcgctggcc
    22681 gccgtcacgt cgcccgccgc catcgcccga gcgggcgcgc tgttgcgaac catcacggcc
    22741 gccgcgctgc tcgccgcgct gctgccgcgc ctgatcaccc cggtcaccgc gttccccgcg
    22801 gcgatgccgg tcgcgacctc cgcggtcgcg ctggccgcca tcacgccggg cctgtccgcg
    22861 tgagcgcgcg gcatgcgcag gcgcagcggg tgccgcggct accggtgccg ctgcggcggc
    22921 ctcatcgccg ctgagccact ggcgcacgcg agcccagaag ccaccggcag gcgcttgcgc
    22981 cgccgcaggc tcgatggcgg gcgcggcggc cgcggccacc ggcgccacga cgatgggtgc
    23041 tgcggtggca ggctggaatg tcggcacggc ggccggctcc agcggcttct gctggccggt
    23101 ggccggatcc ggcagggcag gcggagtcgg caacttgtag gacgacctgc ggaactccgg
    23161 ctgatcgact tcatcgtgcc gcagtctgcg gatcgcgtac tcgggcgtct tgatgttgat
    23221 gttcgggacg agcacgagtt cgacgtcgct cttgtcctcg agcgtgcgca gccactcgcg
    23281 cttctcattg acgaggtagg tcgccacgtc gaccggcacc tcgacgatga cgcgggcggt
    23341 gcggtccttg cgcatctcct cgccgatcag ccgcaggatc gcgagcgtca tcgattcgac
    23401 gctccggatg ctgcctatac ctgagcaacg cgggcaggcg atgtggctcg actcgtcgag
    23461 cgatgggcgc aggcgctggc gcgacatctc gagcaggccg aagcgcgaca ggtggccgat
    23521 ctggatgcgc gcgcggtcca tgcgcaccgc atcgcgcagg cgctgctcga cctcgcgctg
    23581 gctcttctgc gactccatgt cgatgaagtc gatgacgatc aggccgccga ggtcgcggat
    23641 gcgcagctgg cgggcgatct cgtcggcggc ttcgaggttg gtattgagcg ccgtcgtctc
    23701 gatgtcgctg ccgcgggttg cccgcgcgga gttgatgtcg atggaaacga gcgcctcggt
    23761 gtagtcgatc acgaggctac cgcccgaggg cagctggacc ttgtgggcgt acgccgactc
    23821 gatctggctc tcgatctgga aacgcgtgaa cagcggcacg tcgtcggtat agagcttcag
    23881 cttgcgctgc gcgtcggccg gcatcgaccg ctgcatgtac tcctgcgcgt tcgcaaaggc
    23941 ggccgggtcg tcgaccaggc actcgccgat gtcgtctccc aggtagtcgc gcagtgcgcg
    24001 tgagaccggg tcgctctcgc gatagacaag gaagggggca gggcggcctt cgatggcggc
    24061 ggcgatctgc tcccactgct ggcgcagatt atcgaggtcc cactgcagtt cctcgacggt
    24121 gcggccgacg ccggccgtgc gcacgatcgc gcccatgccc tccggcacct gcaatgcgtc
    24181 catcacctgg cgcatctggt cgcggtcctc gccttcgatg cggcgcgaca cgccgccggc
    24241 gcggggattg ttcggcatca gcacgaggaa gcgcccggcg aggctgatga acgtggtcag
    24301 ggcggcacct ttgttgccgc gttcctcttt ttccacctgc accacgacct cctgaccctc
    24361 ggtcagcagt tcgcggatgt tcatgcgccc gccttcgggc gacttgcgga aatactcctt
    24421 cgagacttct ttcagcggca ggaagccgtg acgctcggaa ccgtagtcga cgaaacacgc
    24481 ctcgagcgac tgctcgacgc gtgagatccg acccttgtag atgttcgctt tcttctgttc
    24541 tttggacggt aactcgatcg aaagatcgta gagtttctga ccatcaacca gtgcgacgcg
    24601 caactcttcc tgttgagttg cgttgaccag cattcttttc atttgcccct tctatggatg
    24661 caggggccga ccaggggacg gagaaagatg aatcggacgc gcggatttcg ccagtgaaga
    24721 gctgctgcac gtttttatcg accgcccctt gttgatgagg gcggtgcgcc gaaaaccttt
    24781 ggtccgcctg agcagcgcga tcggcgctcg ggtcggcctg ttgtaagtgc gatggcccca
    24841 attcctggga ccaactaaca tgcggcgcac tcgaatcgac tcatacagcg ttccgtgcgc
    24901 gtcgcgtact ttccaatgat aacaaccgat cttttgaaaa acaatatctt acgccatggt
    24961 tgacgcgtcc gcgccacaac cgggcacgcc gcgcagtgcc gtacggcagg tcgaggccac
    25021 gaccgaagag gccggactgc gcctcgacaa atacgtcgcc aaggccctgc cgcaggtgcc
    25081 gctgagccac gtctttcgcc tcatccgcaa gggcgaggtg cgggtgaacg gcaagcgcgc
    25141 gagcgggtcg cagcggttgc aggcgggcga catcgtgcgt ctgccccccg tgcgggccgc
    25201 ggtcgccccg gtcgacgccg acggcagccc cgccccgcgg cgggtaccgc cccggctcat
    25261 cgaggagatt cgtggggccg tcatgcatcg cgacgagcag ctgctcgtag tcaacaaacc
    25321 ggccgggctc gccgtgcacg gcggcagcgg cgtgtcgttc ggggtgatcg aggcgctgcg
    25381 cgcggcgtat cccgacgata cgctcgaact cgtgcatcgg ctcgatcgcg atacgagcgg
    25441 ctgcctgctc gttgcgcgca aggcgtcgac gctgcgcacg ctgcacgcgc tgcttcgcga
    25501 gcacgaattc gagaagcgct atctcgcgct ggtcatcggc aaatgggatc tcggcaagaa
    25561 gcgtatcgac atgccgctgc gcaccgacct gcgggtcgga ggcgagcgca cggtcaaggt
    25621 gcagccttcc ggcaagagcg cgatcagcga ctttcgcccc gtgcagttct tcggcaaccg
    25681 cgccacgttg ctcgaggtgt cgatccgcac cgggcgcaca caccagatcc gcgtgcacgc
    25741 gtcctatgcc ggtcacccgg tcgctggcga cgacaagtac ggcgaccggg aagcgaatgc
    25801 gatgctcaag gatttcggct tgcggcgcat gttcctgcat gcctcgagcg tgagcttcac
    25861 ttggccgcaa agcggcacgg aattcagcgc caacgcccca ttgccggcag acctcgccgc
    25921 ggtgatcgac aatctcgcgg cggcgcccgt caggggacgg cgtagcccgc gcggcgcaag
    25981 gcgcccgcga gccagatgag cggcaggccg acgagcgccg tcgggtcggt ggtttcgatg
    26041 cgatcgaaca gggcgatgcc gagcgattcc gccttgaagc cgcctgcgca atcgaacggc
    26101 tgctcgcgct cgatatagcg atcgatctcc tcgcccgtga gcgccctcgc caccacgcgc
    26161 gtcgtgtcgg tgtgcgcaag gtcgatgccc gcatcgaggc cgatcacggc gcaggcagtg
    26221 tggaagagcg cccccttgcc ggaaagcgag tcgagctggg cgcggcagcg ctcggcattg
    26281 cccggcttct cgagcagcct ggcgtcgagc gccgccacct gatcgctgcc gatcacgacg
    26341 gccgaggggt ggcggcgggc gaccgccagc gccttgtcgc gggcgaggcg ggccgcgcgg
    26401 gcgggtggca attcgccggc catgcccagt tcgtccacgc ccggagactc gacctggaac
    26461 gggagcgcca accggccgag cagttcgcgg cggtaggggg aggtcgatgc gaggattagc
    26521 ggccggtgaa acaacgactt agaagaggtt ttctttgact cggagggtgc acatacctat
    26581 tatacgcgcc ccatgccgga cggctggtca cgcccactcg acgccgagca actggcggat
    26641 cgccggtcgg acatcgaatt ttcgattccg ttaccggcgc tgcccaggat cgcgccgctg
    26701 ctggcgcgcc ccgagggcag tgcgcgcggc aaggcgtcgt tcgcgcgcga gcagagcctc
    26761 gcggtggtcg acctgacggt cgaggcggag ctgtcgctgg tgtgccagcg ctgcatgcag
    26821 ccgatgtcat ggccggtcgt gggccgggcg catatcgcgc tcgttgggga tgcggatgcg
    26881 gccgatgcgg tcgcgtcgga atttgagacg gtgatcgcgc ccggtgggcg ggtcacggtc
    26941 ggtgaactcg tcgaggaaga gctgctgctc gccttgccgg tcgtcgcgct gcatgcgcgt
    27001 gcagacgcct gcggcgtgca gctcgacgcg gagccggaca aagaagtaca gaaaccgttt
    27061 gcgggactcg ccgatctgtt gaaacgcggg ccgtaatcca ggagtagtag agatgcctgt
    27121 tcagaagagc cgtaggacgc catcgactcg cggcaagcgc cgggtgcacg atcgcctcgc
    27181 ccgccccgcg ctgtccatcg acccgcagtc gggcgaaacg cacctgcgcc atcgcatcac
    27241 gccggacggt ttctaccgtg gcaagcgcgt gatcgagaag cctgtcgaga gcgaaggcga
    27301 agagtgagca ccggcgccct gcccggcgcc gcgttgaacc cccgccggcc gacatcgcgt
    27361 gcccacgctc gcgattgacg tgatgagcgg cgacctcggc gccacggaat gtgtgccggg
    27421 agccttgctc gcgcttgcgc ggtcgccgga tctgcgcgtc gaactggtcg gatcccccga
    27481 ggcgatagag ccgctgctcg gcgcggccga cgccgcgctg cgtgcgcgcc tcgccatcgt
    27541 gccggccgcc gacgtcgtgc ccatggacga gctgccccgc gctgcaatcc ggcaccgcaa
    27601 gcactcgtcg atgcgaatcg cggtcgatcg ggtgaaggag ggcgccgcgg tcggttgcgt
    27661 cagcgccggc aataccggcg cgttgaccgc gatcgcgcat ttcgtgctga agaccgtgcc
    27721 ggaggtcgag cgcgcgccga tcatgtccgc gctgcccgcg gcgcacggcc acacctacat
    27781 gctcgacctc ggcgccaaca tcagcgcgag cgccgtgcag ctgtgccagt tcgcgatgat
    27841 gggctcgatc gtcgcgcgcg acgtctacgg tgtcgccgag ccgcgcgtgg gcctgctcaa
    27901 catcggcgaa gaagacatca agggccatga caccgtgcag tccgccaacg cgctcatcgc
    27961 ggcgagcggc gtgcgctacc tgggcttcat cgagggcgac gacatcttct cgggcgaggt
    28021 cgacgtcgtc gtgaccgacg gattcaccgg caacgtggca ctgaaatcga tggaaggcct
    28081 cgcgcgcctg atcaccgaca cgatgcgcgc ggagttcacg cgctcggccc tgagtcgcgc
    28141 ggcagcgctg gtggcgcgcc cggtgctgaa gcgcgtcggt ctcgccctcg atccgcgccg
    28201 ctacaacggc gcctgcatgg tcggcctgcg tggagtcgtg gtaaaaagcc acggacgcgc
    28261 cgaccgtacg gccttcgccc gcgcaatcga gctgacccgc acagcggcgc tgcacgatct
    28321 gcccggctgc gtggcgcggg cgttgggcgc acaaccgcca caggggtcct gatgtattcg
    28381 cgcattgccg gcactggcag ctacctgcct gagaaggtcg tcaccaactt cgatctcgcg
    28441 aaggtcatgg acaccaccga tgagtggatc cgcgagcgca ccggcatcgt cgagcggcat
    28501 atcgcggccg agggcgaggc cacggtcgat ctggccgaga aggccgcccg gcgcgcgata
    28561 gaggccgcgg gcatcgaccc gaaggagatc gacctgatct gcttcggcac aaccactccc
    28621 gatcttgtgt tccccaactg tggcgtcctg ctacaggggc gcctggggct gcgcggctgc
    28681 gcgtcgatgg gtctcgaggc cgcgtgcacg ggctttctct actcgctgtc gatcgccgac
    28741 aagttcatcc ggctcggcga ggcgaagtgc gcgctggtcg tcggcgccga gacgctctcg
    28801 cgcattaccg actggactga ccgcggcacc gcgatcctgt tcggcgatgg tgcgggtgcc
    28861 gtgatcctga agcccgatac cgaggctggg gtcgtctcga cgcatctgca tgccgacagt
    28921 acctacaagg acctgctgta ctgcccggcg ggcgtgtcga agggcattgg ccggcccggc
    28981 gatccgccga tgcacataca catgtccggc aacgagacct tcaaggtcgc ggtgaacacg
    29041 ctcgggcgca tcgtcgatga gactctcgcc gccaactcgc tcgacaagtc ggcgatcgac
    29101 tggctcgtgc cgcaccaggc caatatccgc atcatccagg ccacggcgcg caagctcgac
    29161 atgccgatgg agcgggtgat cgtgactgtc gaccggcacg gcaatacgtc ggcggcgtcc
    29221 gtgccgctcg cgctcgacac cggcgtgcgt gacgggcgca tcaagcgcgg cgaactgctg
    29281 ctgctcgagg ccttcggcgg cggtttcacc tggggctcgg cgctcatccg gtactgacga
    29341 tgacagcgcg cacggtggcc ttgggactcg cgttcgtcca gggcatcgtg ctgtggtggc
    29401 tcacggccag tgcgcgcgcg aaatcgtggc cggccaccga ctcggcaatg ctggcggcgc
    29461 tgctcacgcc cgcggtattc gtcccgccgc ttgtgtacct gcaactcggg cttgcgcatc
    29521 tgcgcacggt cgcgctggtg tcgctcgcgg cggggctcgt gctcggggcg ctcggctggc
    29581 actacggcgc atggatcgcc ggcgccggtt tgcccaccgg cggactgttc ggcgaggacg
    29641 ccttcgcctt cgcgatcgcg gtgctggtac tttgctttca tgcgctgccg ttcctgcaat
    29701 ccgcgctcgt ggcgcggcgg cagtggcccg cctatccgat gttgttcgaa ttcgcgtggc
    29761 gcaatgcgct gcagatcgcg ctcgcgatgc tgttttgcgc cgcgctgtgg gcgctgctgg
    29821 cgctgtggat gggcctgttc cgcatgatcg gcatcgactt cttccgggag gccttcaccg
    29881 acccgcgtgt ggcattcccg ctgaccaccg tggccttcgc cacggcgctc gcgctgtcgg
    29941 gcccggtcga gcggctgcag caggcgctgc gccagcagtt gctggcgctc ttcaagtggc
    30001 ttgcagtgct tgcgatgctg atcctgacgc tcttcagcat cgccctgctg ttgcgctcgc
    30061 cggagctgtt cggcaccggt cggcgcgcga tcagcgccgc gtggctcctg tggctgctgg
    30121 tcttctgcgt gtacctgctg aacgcggcgt accaggacgg tggccatgcc gcgccgtatc
    30181 cgcggccggt aggcgtggca ctgcgtgtcg cgacaccttt gctcgtggtc gtcgcggtgc
    30241 tcgccctcta cgcactcggg gtgcgtgtcg gcgcctatgg actcaccgtg ccgcgcatct
    30301 gggccatgct ggcggcggcg ctcgcgctcg tctatgcggt gggctacacg atcgctgcga
    30361 cgcgccgcgg cgcatggatg gcgtcgatgg gtgccgtcaa tgtcgtcgcg gcggtgatgc
    30421 tgatagtcgc gctgctgctg atgctctcgc ccgcactgtc gccctaccgg ctcgcggccg
    30481 acagccaggc cgcacgcgcg ctgcgcggtg cggacggttc gacggccgcc cgcgggccgc
    30541 atgtacggta tccgggcagc tatgccgatg aatatgtgac gctcgctttc gacacgggcg
    30601 cctacggacg gcagtggctc gcaaggttgg ccaggctcga aggacacccc gatgccgccc
    30661 ggattcgcgc gcgggcagcg accatgctcg caagcaggca acgctacgat gtcgcgccgg
    30721 cactggcggc caatttaccg atgacgctgg tggcctttcc ggaagggcgc gaaatcgacg
    30781 ccggcctgcg cgcagcgatc gagggcgcgc aagtcccgcg ctggctcggt ccgttgcgca
    30841 gcagcgaagt gggttcgtac ccggtgctgt tcgccgacct cgaccaggat ggcgtcgagg
    30901 aagtgttcgt cttctcggag ccccaggcct tcatgctgac gcggaaggac ggtgtctggc
    30961 gcgagaccat gctgctgggt gggggcgtgt cccggacgca cgcgctggag cgtctgcaag
    31021 ccggtgacta ccggctcgtc gcaccgcgct ggcagaaact cgaagtcggc ggcgtcacac
    31081 tcaccgtgat gcccgaccag tagataaaaa aaacgccgcg cagggcgcgg cgtttggggt
    31141 cgagcgaagt aaaacttact tcgagatcga acgcttgaac atgcggtcga tcttctcgtt
    31201 ggtcgcgtca cagcacgact ggctggcctg cgcagcggca agcgcctggt tcgcggtgct
    31261 ctgtgcgccg ctggcggtcg aggaggcaga ctgggcagcc gaagaggccg catccgccgc
    31321 gctcttggcc gcagcagtgt ccgatgccag ccggccaacc tgtgccttca gatcatcgac
    31381 ctgcgcctgc agcggcttga ggtcggtgca gccggccagc atgacaacag cggcaacggc
    31441 cgcaacttta acgatcgcgc tcttcatagg agtccccttc atgtggatac agaaacctgt
    31501 gccggccgaa cgttatcaca ttgcgatttg tgcggcggcc acgcgacgca actgcaacaa
    31561 attcggtcgc tgattgcaag attcgcgatc ctacacctgt gtcggtgctg tccgacacgt
    31621 tgcgcatgcc ccacgcttcc gcatgacacg acaatcgact cgaccgcaag ggtgcagtca
    31681 tctgcgaccc tcgggttcgc ggccttttgg cctcgatgct gcggcctctt gcgcggcatg
    31741 aaaaatcctg tatagaatcc caccctgtac atccatacag ccaccatcac agcagcagca
    31801 tcatgtccga cctcacgccc cgccagaccg aaatcctgcg tctgatccag cgcaccatgg
    31861 ctgaaaccgg catgccgccc acccgcgcgg aaatcgccgc cgaactcggg ttccgctcgg
    31921 ccaatgcggc cgaggagcac ctgcgtgcac tcgcccgcaa gggcgtcatc gcgctgatcc
    31981 ccggcacttc ccgcggcatc cagttgaaag acacgatgcg cgagcagctc ggcctgccgc
    32041 tcatcggccg ggttgcggcc ggccggccga tcctcgcgga agaaaacatc gaggcgcgct
    32101 accagatcga tccgggcatc ttccagccga agccgcacta cctgctgaag gtcaccggca
    32161 tgagcatgaa ggatgctggc atcctcgacg gcgacctggt cgccgtgcat cgcacgcccg
    32221 aggtgcgcaa ccggcagatc gtcgtcgcgc ggctcgagaa cgaagtcacc gtcaagcgct
    32281 accggcagga aggctccatc gcctggctgc tgccggaaaa tgccgacttc gagccgatcc
    32341 gcgtcgacct gaagcaggaa cacatgatca tcgagggcgt cgtcgtcggc gtgctgcgcc
    32401 gggggaagat ctcatgagca tccagggact gcgtaccgct gcctaccatg tcggcgatct
    32461 ggccgcggcg aaggcctggt attcgcgcgt gctcgagcgc gagccgtatt tcgaccagcc
    32521 gttctatgtt ggctacgaag tcggcggctt cgagctcggc ctcgttcccg acggcaagcc
    32581 gggcccgggc ggcacggttg tgtactgggg cgtgcccgac gcggcggcgg agctgcgccg
    32641 gctcgagtcg ctcggcgccg ccgtgcgcga tccgctgcag gacgtgggcg agggcatcct
    32701 gaccgccacc gtgaccgatc ctttcggcaa tgttttcggt gtcatcgaga atccgcattt
    32761 ctcgctcgcc aaagtccgct gatgcgcacg ccgcgctgcc attgcatcga tgtccgacga
    32821 cccgctggct cggctcctcc agcatcccgc attgttgcgc gggcgcagcg ccgcgcagct
    32881 gcccacctgg tccacggggt ttccgtcact cgatgccagc ctgccgggcg gcggctggcc
    32941 gcgtgcgggt ctcgttgaac tgctgacgcc gcgtcccggc gtcggcgagc tgcggctgct
    33001 cgttcccgtc ctggcgcaac tgtcagggct cacgcctgcg cgctggctcg cgtggatcgc
    33061 gccgccgttc gaaccattcg cgccggcgct cgccgagcgc ggcatcgcac tcgaccggca
    33121 actgatcgtg cgcaccgcag cgccgctgtg ggccatggag caggcgctcg gctcgggtgc
    33181 ctgtgaggcg gcgctcgcct gggcgccgcg tgtgcgagcg cgcggcctgc gtcgcctgca
    33241 gctcgccacc gagcgcggtc gtaccctcgg catcgtgttc cgcgaggcca gcgccgcgcg
    33301 cgagccgtca cccgctgcgc tgcgtctcgc ggtcgtgccg cacggaggcg gagtggaggt
    33361 gtcgctgctg aaaagccgcg gcggagcgcg cggggcaatc cacctgcagt tcggggatgg
    33421 tcgtgccgaa gagcgctgag gacctggagt tcgaggcggc cggccgggga tcgccggtcg
    33481 gcgctgccgc gctccggcgt ccgacgccga gcccggcatg ttctcccgcg cctgcgcccg
    33541 cgcccgcgct cagcgcagag gcgggcgagc tgtggctggc cctgcacctg cccacgctcg
    33601 ccttcgatgc cgtgtgccgc gaaggcgatg cctcgcagcc ggtcgctgtc gtcgagccgg
    33661 tgcggcgcgt gcagcggatc gtgatcgcga gccgcgccgc gcatgcacga ggtgtcgcgc
    33721 ccggcatggc gctcgcggcg gcgctcacgc tcgagccgcg gctcgatacg cgcaaccgtg
    33781 acacgggcgc cgagcgcgcg ctgctcgagc ggcttgcggc ggcggcgggg gagttcactt
    33841 cgcgagtcag cctcgaaccg cccgacggcg tgctgctcga agtgcgcggc agtctcgcgc
    33901 tgttcggcgg cgtcgatgcg ttgtgcgcgc aggtgagcgc ggcgtgccga cgggttggca
    33961 cgcgcgcacg gctcgcgctc gcgcccacgc cgcttgcggc gctcgccggc gcgcgcgtat
    34021 cgcgcggtcg cgccgccgcg ccattccggg tcatgcaccg cgcgcaactg gtgggcgcac
    34081 tcgcgccatt gccgctcgcc acgctgcgcc tgccgcagga cacactcggg cggctcgcga
    34141 agatgggcgt gaacgggatc ggggccgcgc tgcgcct
//
LOCUS       DAOQVY010000009        33440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_1520_length_33440_cov_5.902471, whole genome shotgun sequence.
ACCESSION   DAOQVY010000009 DAOQVY010000000
VERSION     DAOQVY010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 33440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33440
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1520_length_33440_cov_5.902471"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PKN91_01805"
     CDS             complement(<1..484)
                     /locus_tag="PKN91_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26834.1"
                     /translation="MVNNDRILRLSALLATLIVPPAYAGPWLAPGDPAMRHDIEMLAD
                     AGILRGPITTWPMSWPDVARSVNAAQLPDGASAALRQSLMRVQSAARREARSGLDGLA
                     VRVSGAHDPDPLRTFADTPREEGELELRASWMGDRLAAKLQVTGVTDASDDQSARFDG
                     S"
     gene            575..1618
                     /locus_tag="PKN91_01810"
     CDS             575..1618
                     /locus_tag="PKN91_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26835.1"
                     /translation="MRILFVPVSATTGSGEYVRSLAIAAALRAMTNAEISFVLNRNAP
                     HAATTPFPALLLPSSPTFHPREVSALIRERRPDLVVFDNAGRTAQLRAARESGARVVY
                     ISSRPRQRRKAFRLKWLPLLDEHWIAWPEFMAGPLSGSERLKMRYGARRPTVRFFDTI
                     VPTPTDAESAAVAARFGLEPEQFALVVPGGGGAHPGARQGPRTMAAAALRLARTGHPT
                     LLVGPHGEVAADGSGLRYSPHLPIQDLAALMARARVVVANGGDTLLQALSFRRHCVAA
                     PLAHDQVGRIRHCADAGIARGVDLDEVEVARAAEELLNDGSLRAEQAARIAQRPIRNE
                     LEFAVGALARLAA"
     gene            1644..3584
                     /locus_tag="PKN91_01815"
     CDS             1644..3584
                     /locus_tag="PKN91_01815"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HNS26836.1"
                     /translation="MALLAGFVGMAQARGVSPYLPLSIEPEMERQIEQVLTLADKPFL
                     TRPIAAAVVWDALPAACARDEALCRSVERYLRRYMGRAGVAEASIAAGHADGSARPVP
                     NHHGLETESPWEASARVYWQPFDHALLTLGGVAYDGEATPTGSLLSLGWDFAQLDMGF
                     RDHWLSPFTDSAMLLATEAPTMPSVTISNYMPLTRLGLHYEVFAAQMSGSDAIVFEGG
                     LTSGEPNVAGLHLSIEPASGWSLGFNRVMQFGGGDRDDYSFGDVIDAFFNPSSHDNAN
                     DPEDSEFGNQVASITSRFVFPGAVPFSVYAEYAGEDTSRGRNYLLGNSALSVGIDFPL
                     LWERFDLTFELSEWQNGWYVHHVYGDGLTNEGRVLGHWGADQRVFNDDVGAYSAMARI
                     GWRPAFGGSFVLRGRVIENESYSAAAYEQGYDLALSYARPVGAFSLGGEVYAGRDVFG
                     EDFSRVTAFVRYAPEGAAGMGHTVMSTASGRRERASGAELFVDLGVNANQVRIDLDDD
                     LPRATSDTEIALHLGVGARRQVTDRQDLGVRLEYDEIDGHALIGVRALDYRYRIKGPF
                     ALTGFAGAARYDLATPAYGLYIGAGAQWRGVLRSDFDLSLDLRYASKVARDDLVADDP
                     VGGRPDSFYDIASATLYLSWRF"
     gene            complement(3616..4404)
                     /locus_tag="PKN91_01820"
     CDS             complement(3616..4404)
                     /locus_tag="PKN91_01820"
                     /inference="COORDINATES: protein motif:HMM:NF016459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS26837.1"
                     /translation="MTLTTIHMFWHGPPLSRLERLAVASFLHHRHAVELYVYDEPRGV
                     PAGVQLRDAAEVLPRDALFHHRRTGSVALFADWFRYRLLAARGGIWADMDVVCLQPIA
                     YPQAVVFAWEDAHQVNNAILGLPAGHALARWLADCCEHPNRIQPYDGWRMRLRKLRRR
                     YLQGDRRDRLRWGELGPKGLTAAARHLGCLERALPSAHFYPVPAQHWRTLFEPAPDGA
                     LPWGAESRAVHLWNNMAARSPGFDKDARFAADSPFEVLCARYGV"
     gene            complement(4495..6105)
                     /gene="pgi"
                     /locus_tag="PKN91_01825"
     CDS             complement(4495..6105)
                     /gene="pgi"
                     /locus_tag="PKN91_01825"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNS26838.1"
                     /translation="MSAQAPQPPRVAGPETRPWQRLRELTSTSGARHLRELFAADAKR
                     FEQFSARTANLLLDYSRQRVDTDVLATLAALAEAVGLRESIAAMRRGEQINTTEKRAV
                     LHVALRQREGQQLGGAQVERLVLAERARMLDFAERMRARYRTVVNIGIGGSDLGPAMA
                     VQALQRYTDGAPRLEFVSNVDGCQIADVIATADPATTLFIVASKTFTTQETLANARAA
                     REWIAGTLGEAAVPDHFAAVSVNTAAMNDFGVHPDYRFEMWDWVGGRYSIWSSIGVSL
                     AIAIGRERFLAFLDGGRDIDEHFASAPWHTNLPALAALLGVWNGNFLALPTLAVLPYD
                     QRLARFPAYLQQLEMESLGKSVTQGGTAVQWTTGPVIWGEPGNNAQHSFFQLLHQGTP
                     RAALDFLLPARSSCGRQGQHDLAIANCLAQAEAFAFGQPLEVAGSVHKVHGGNRPLSL
                     LAFDALDPATLGRLIAHYEHKVFAQSVIWGINAFDQWGVELGKKLCAGLVPAVTGEGR
                     PGSGSDAGLAAAPEHLRPLLATLRGWKQ"
     gene            complement(6102..7265)
                     /locus_tag="PKN91_01830"
     CDS             complement(6102..7265)
                     /locus_tag="PKN91_01830"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNS26839.1"
                     /translation="MKVPLLDLKPQYRALKRELDAAIEAVSESQYFILGPAVKKLEAD
                     VAAYTGTQFGIGVSSGTDALLVALMVLDIGPGDEVITTPYTFFATAGTIARVGARPVF
                     CDIEPGSFNLSPQAVSHFIERECESRDGKLVNKRTKGVIKALMPVHLYGQVADMDPLM
                     ALARKHGLRVIEDAAQAIGAADAQGRRACSFGDIGCLSFFPTKNLGAFGDAGMCVAND
                     AALAERLRIFRVHGGEPKYYHAFIGGNFRIDELQAAVLGVKLPHLDAWTAGRQRNAAY
                     YDAAFARAALGDAVVTPPAAPGARHIYNQYVIRVRDRDALRSHLAASDIGTEIYYPVP
                     LHLQECFAYLGHGAGDFPQSERAAADTLALPIFPELETAQLQFVVDRIAAFYR"
     gene            complement(7276..8319)
                     /locus_tag="PKN91_01835"
     CDS             complement(7276..8319)
                     /locus_tag="PKN91_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HNS26840.1"
                     /translation="MKVLVTGAAGFIGSHVAQRLLARGDEVVGLDNLNDYYDVNLKRA
                     RLARLEPQPGFRFVKLDLADQGGMTHLFKREKFKRVVHLAAQAGVRYSLENPHAYVSS
                     NVTGTLNVLEGCRHNAVEHLVFASTSSVYGAHTDMPFSVHSEVGHPLSMYATTKRANE
                     LMAHNYAWLFGLPVTGLRFFTVYGPWGRPDMALFLFAKNILAGKPIDVFNHGHHRRDF
                     TFVDDIVEGVVRATDRIAQPNPAWDSAHPDPATSSAPFRLYNIGNNRPVELRRYIQVI
                     EECLGRKAEMNLLPLQPGDVPDTEADVQDLVEDVGYKPDTPIETGVRRFIDWYLEYYK
                     GGPPAESRVTNLG"
     gene            complement(8327..9604)
                     /gene="tviB"
                     /locus_tag="PKN91_01840"
     CDS             complement(8327..9604)
                     /gene="tviB"
                     /locus_tag="PKN91_01840"
                     /inference="COORDINATES: protein motif:HMM:NF011729.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HNS26841.1"
                     /translation="MVSLRKCRIGVVGLGYVGLPLAVEFGKHYETVGFDIKPERIAEL
                     SRGRDSTLEVSAAELKSSKRLSFTSDLATLRKCRVFIVTVPTPIDDYKRPDLTPLVKA
                     SETIGKVLKKGDVVVYESTVYPGCTEEECVPVLERVSGLKFNKDFFAGYSPERINPGD
                     KQHRVTTIKKVTSGSTPEVAEFVDKLYASIITAGTHKASSIRVAEAAKVIENTQRDVN
                     IALINELALIFNRLGIDTEEVLLASGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQEI
                     GYHPEMILAGRRLNDNMALYVAGEVARLMSDRRIHVKGSRVLVLGLAFKENCPDLRNS
                     KVADVVRELGKFGAKVDVYDPWVDPREAKHEYGIRPVKAPKKGAYDAVVLAVGHQQFK
                     DMGPAAVRRFGRRNSVVYDIKYVFPADQVDGRL"
     gene            complement(9635..10393)
                     /gene="dnaQ"
                     /locus_tag="PKN91_01845"
     CDS             complement(9635..10393)
                     /gene="dnaQ"
                     /locus_tag="PKN91_01845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HNS26842.1"
                     /translation="MRQIVLDTETTGLEVERGHRIIEIGAVELRNRRATGRNFHRYLN
                     PERAIDEGARAVHGISSADLADAPRFAQVAAELIEFISGAELVIHNASFDVGFLEAEL
                     QRLPESSGLPRRIGDLCGVLDTLALAREMHPGQRNSLDALCRRYGIDNSHRELHGALL
                     DARILADVYLTMTGGQSTLALDSQHTPGAPGSQGGEVQALVRPAAGLVVRQATAAELA
                     DHERMLDLIARASGDRCVFRQCDVDRVRTAANRQ"
     gene            complement(10405..10854)
                     /gene="rnhA"
                     /locus_tag="PKN91_01850"
     CDS             complement(10405..10854)
                     /gene="rnhA"
                     /locus_tag="PKN91_01850"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNS26843.1"
                     /translation="MSAVTIYTDGACRGNPGPGGWAALLSSGAHRRELSGAERLTTNN
                     RMELTAVIRAFEALKRPSVVDLHTDSEYVKRGISEWLPGWKARGWKTAARKPVKNQDL
                     WEALDAAQARHKVRWHWVKAHSGVPGNEHVDRLANLAIDALMRDGIE"
     gene            complement(10851..11495)
                     /locus_tag="PKN91_01855"
     CDS             complement(10851..11495)
                     /locus_tag="PKN91_01855"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNS26844.1"
                     /translation="MTPAATCPDSWLRGTPGAALLEAESRLLAEALADVFGWELLQIG
                     AWGPPRGLIRSARTRQQTVVVEREAPGVDVVARLPQLPIASGTVDAVLLPHTLEFLPD
                     PKTMIREVDRVLVGEGQLIVLGFRPFGLWGLRARAARRSFPPGLKGLLAERRVSDWLA
                     LLGYEIVASRHFHHGVYLLRARKRVYTMTPIRPRFRERSAVIGSLVKPTSRSGS"
     gene            11729..13213
                     /locus_tag="PKN91_01860"
     CDS             11729..13213
                     /locus_tag="PKN91_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNS26845.1"
                     /translation="MPEPEPLVAPAPEVPVTVIGITPEKYSDLFDRIRAGYQLEDVDS
                     RQVDREFRFYANNPDYLGRVFNRAAPYLYHIVQEIEARQMPLELALLPVVESAFEPYA
                     YSVARASGLWQFIPPTGSRYGLKQNWWYDGRRDVVESTRAALDYLQFLHSEFDDWLLA
                     VAAYNCGEANVARAIRANKAAGKPTDFFSISARLPKETRAYVPKLLGMSRLFLSPQDY
                     GIEFSSLPNEPYFAQVETLGQIDMKLAAELAGISYEELYTLNPAFHRWATDPAGPHYL
                     LVPADTADLFRESVRQLTADERMRVAHHRVQPGDTVADIARRYHTQTHVIRELNDLTA
                     DAKLMVGDDLRVPSGVTELPAKVQLAALRVDRGTRARGGVHVVRRGDTLSAIARRNRL
                     SVRALAAMNNIGVNDTIRAGQRLVVNKSAAASRAAPTRTASANHGAGAGSGGSSYTVR
                     KGDSLWSIARRFGVSVSQIVAWNGISSESTIKPGQKLTVKKVPG"
     gene            13226..14011
                     /locus_tag="PKN91_01865"
     CDS             13226..14011
                     /locus_tag="PKN91_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009390813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HNS26846.1"
                     /translation="MAGKRALIVGVATERSIAWGIAQAMHREGAELAISYANDKLRER
                     VVPLAAELGALTLPLDVTNDAEIDGAFDLLKREWGSLDVLVHAVAYAPREALTGGFTE
                     ATSRESFRIAHDISSYSLTALARGAAPLMAGRNGAIVTLSYLGAVRSVPGYNVMGVAK
                     ASLEASVRFLAADLGPQGIRVNAISAGPIKTLAAAGIPGFRKMLGRVAEAAPLRRNVT
                     LDDVGNSAAFLCSDLAAGITGEILYVDSGYSTVGMSFPLETDG"
     gene            complement(14025..15863)
                     /locus_tag="PKN91_01870"
     CDS             complement(14025..15863)
                     /locus_tag="PKN91_01870"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNS26847.1"
                     /translation="MLQRIRENLNGPLAYFILGAISLVFVAWGAYGIVDVGIGQSAYA
                     AKVNGEKIPLETARQAWLEQQAQAVQAFGGEIPEDSKAQLQQSVLEGLITRAMLDARL
                     HDLGYRVSDEQIKTAIQQEPAFQVDGKYDASVAKARLAQVGLTVAGFEADLRTSLARQ
                     QLQRAIALSDFVTPAEVDRIIALEDEQREVRYALFKASDYAPAAVGDDAVRAYYEANT
                     AKFMTTESVRLAYGELRLDQVATQITVVDDDLRRLYAENRDRYTTPETRRARHILVDD
                     EKTAQDLLAQLNAGGDFAALAAANSKDSVSAANGGDLGYATRETFVAPFADALFGMQV
                     GETRGPVKTQFGYHLIRLEAIEPGKSKTFEEARSELDAQYRRDEAADRFGDAQEQLQA
                     RTEQPGVTLDALVQEFSLSTGKVEVYERGSGGGTLGTDPALEDIVFSDTVLNQGRVGG
                     PVGLGEDRLIVVQVLEHRAPQARPLAEVQASIVSTLKHEQGMAAARAAADAALARLRK
                     GEQLDAESRQIARIDPSVPAEIRASVFSMPHPDGEPIQSVVPTADGAAVVALLGVKSD
                     VPPLSPEARQERLQQIVARVGSADVIAYVAEARQNAKVSVNPKAFE"
     gene            16200..16634
                     /locus_tag="PKN91_01875"
     CDS             16200..16634
                     /locus_tag="PKN91_01875"
                     /inference="COORDINATES: protein motif:HMM:NF021536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HNS26848.1"
                     /translation="MHPTTAELFTKTLEKTGRWIDDLMTEMGTSDPRQAYSVLRAVLH
                     ALRDRLTVDEAVNLGAQLPMLVRGFYYDGWRPEGRPDRYRHKSEFLLRVANAYGGLSA
                     TEQEPAVGAVFRLLSRHVTEGEIRHVRDQLPAEVRDLWAPSA"
     gene            17015..17476
                     /locus_tag="PKN91_01880"
     CDS             17015..17476
                     /locus_tag="PKN91_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS26849.1"
                     /translation="MTKQVSCCSADNTLAQAAQLMWDHDCGCLPVYSGDGASRLAGVI
                     TDRDICMSALFKGKPLHELHVSDAMSRQLQTCRPGDSLVDAERTMRQARIRRLPVVDD
                     EGSLVGMVSLADLAREAAREKSLSRQEITETEVGDTLAAICQPQAQRQLAA"
     gene            17707..19437
                     /gene="polX"
                     /locus_tag="PKN91_01885"
     CDS             17707..19437
                     /gene="polX"
                     /locus_tag="PKN91_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HNS26850.1"
                     /translation="MPVHNEDLAAAFDEMADLLALKAENRFRINAYRRAAQVVRALPE
                     ELPIKIAAGFDPDSLPGIGADLADKIRAFVKTGELPGLAALRRSVPAGLREIMNIRNI
                     GPRRAVALYDALGVHDLETLRRALEKHRVREVRGFGAKMEERLTKAVEETPGRSGRLL
                     RSVAAQYATSLESYLRALPGVTKVDVAGSFRRGRETVGDLDFLVCSEHPLDVGGALAK
                     YDETTTLLAKGPTRSAAVLKSGLQVDVRLVPSESYGAALYYFTGSKAHNVHVRRLAMA
                     RGLKLNEYGVFRGRKRIAGDTEASVFASIALPCIEPELREDRGEIEAAQARALPTLIT
                     RRDLRGDLHVHTKATDGTASLEAMAEAARKAGLEYIAITDHSRHLGMLRGLSADRLAR
                     QLNEIDVLNASLDGLTLLKGIEVDILEDGSLALPDSVLKRLDVVVASLHDHFGLSRRR
                     QTDRLLRVIDRPYVSVLGHPMSRLIGERAAIECDWARLFRRAGERPIYLELNSQPARL
                     DLDDVLVREAAANDVLISIASDAHGIGSFDLLGGGVLQARRGWLSKGQVLNTRPLQEL
                     RRLLRRTVLA"
     gene            19618..21504
                     /gene="ftsH"
                     /locus_tag="PKN91_01890"
     CDS             19618..21504
                     /gene="ftsH"
                     /locus_tag="PKN91_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNS26851.1"
                     /translation="MGKLKISVWDVLLLLGAVMAMYSLTTLRTTATLPYSEFKALVAA
                     GRVAEVTLGDQQIAGRIKAGPLEDVLSPQSLAKLVPDSEGERAFTTIAVNDPDLVQQL
                     DAAHVQYSGGRSANWLMTLLSWVLPALIFVGLWSFMLKRMAGGLGASSGLLDVGKSKA
                     RLYMEKLVNVTFADVAGVDEAKEELQEIITFLRDPKQYGRLGARIPKGVLLVGPPGTG
                     KTLLARAVAGEASVPFFSISGSEFVEMFVGVGAARVRDLFTQARAKAPCIIFIDELDA
                     LGRARGFGPAGGGHDEKEQTLNQLLAELDGFDPSSGVVLLAATNRPEILDPALLRAGR
                     FDRQILVDRPDKTGRVQILNVHLRKIRIDTSVKVEEIAAMTPGFTGADLANLVNEAAL
                     LATRRNADLVAMQDMSNALERIVAGLEKRNRLLNETERRTVAYHECGHAIVSMLTPSA
                     DRVQKVSIIPRGIGALGYTLQRPLEDRFLMARHELIERMIVLLGGRAAEQLLCSEVST
                     GAADDLVKATDIARHMVKRYGMSEALGPVSYDSAATPLLGPAADYYRPRDYAEATAGR
                     IDTEVRELIEEAARRAHELLLAHRGTLESMAAELLARETLDAQAIEKFRAELTRSTAL
                     VRTA"
     gene            complement(21541..22662)
                     /locus_tag="PKN91_01895"
     CDS             complement(21541..22662)
                     /locus_tag="PKN91_01895"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNS26852.1"
                     /translation="MTLFLCGDVMTGRGIDQILPHPSAPQLYEPHVRSACEYVELAER
                     AHGRIARPADFAYIWGDTLLELERRRPALRVVNLETAVTSSSDAWPGKGINYRMNPRN
                     VGCLTAADLDCCVLANNHTLDWGREGLTETLRVLREAGLSVAGAGANLAQARTPACFD
                     LPDRQRVLVFACGAPDSGISPEWNATEYRSGLCVLDDLALHTVEELAAHIRREKGGGG
                     VVVMSLHWGGNWGYEVSERHRRFAHQLIDVAGVDVVYGHSSHYPKPIEVYRGKLILYG
                     CGDLLNDYEGIGGRERFRSELGLMYFPEIDCDTGNLVRLSMTPTRIRRLQVKLADEGE
                     ARWLSATLSRECESFGTSVRLLPEGRLALELTEVVDAIA"
     gene            complement(22689..24515)
                     /locus_tag="PKN91_01900"
     CDS             complement(22689..24515)
                     /locus_tag="PKN91_01900"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNS26853.1"
                     /translation="MPDPLEIELSRRVWAQRYQYRCGDVAVDTDIAHTWRRVARAAAT
                     VEGVSADLWEAKFAELLRGFRFLPGGRILAGAGTELRATLLNCFVMGAIEDSMDGIFS
                     ALREGALTMQQGGGVGYDFSTLRPRGERARATGNIATGPVSFMEVWDAACATITGSGP
                     RGGAMMATLRCDHPDVLQFIGAKHRSRVLSHFNLSLQVTDEFMQALAADEPWPLVFPR
                     SALLPGERSLPTVTRSWGGPEPTECAVLRTVSPRSLWNALLEASFDCGDPGVLFVDTI
                     NRENNLHYCEWLSATNPCGEVPLPPYGACDLGSLNLVRFVRDPFAASASIDVAALTAA
                     TATAVRFLDDILEVSRYPLPQQREQALRTRRVGLGVTGLADALIMLGLRYDSAAAREQ
                     AASILRSIRDAAYESSIELAVAKGAFPAFDRDGFLASQFVERLPARLRDAIAQYGIRN
                     SHLVALAPAGSISLLAGNVSSGVEPVFAFEGVRRTRTVDGWERHTTSDYAWRRWRELH
                     GAAPPGDAFVTAEDLDPEAHLAMQAALQPFVDGAISKTINLPRDYPFERHREIFAAAH
                     RLGLKGCTVFRSTRALQGVLSGESGQSALVANECRLSTWGSS"
     gene            complement(24681..25151)
                     /locus_tag="PKN91_01905"
     CDS             complement(24681..25151)
                     /locus_tag="PKN91_01905"
                     /inference="COORDINATES: protein motif:HMM:NF037928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HNS26854.1"
                     /translation="MRIRVAVADRAQVLFYDAERPESKLRLAGQMTDPEARLRDRDYK
                     SDRPGRVFDHAPGGKRRGAVAHHSTGGERTPSKHEAGVFARRIVDELERAQRQNHFDR
                     LVLMAAPEFLGDLREVMPKSLRQAIALEIDKNLVHEDELAIREHLPADVFVQRL"
     gene            complement(25192..26622)
                     /locus_tag="PKN91_01910"
     CDS             complement(25192..26622)
                     /locus_tag="PKN91_01910"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNS26855.1"
                     /translation="MDVALFNRRSEHVWEIAPRGPMRVPAVIYASEPLIRAMDQKVYE
                     QAVNVATLPGIVEASYAMPDAHWGYGFPIGGVAAFDPDAGGVISAGGVGFDISCGVRC
                     LHTGLTRPDILAVQKELADMFFYRIPAGVGSTGLIHLNVQEMEAMLTGGAHWAVHAGW
                     GVAEDLERIEEHGQMLHAKPENVSQHAKNRQRDEMGTLGSGNHYLEVQEVTSVYDAQI
                     AQAFGLEVGDIVVMIHCGSRGLGHQIGTEFLKQMAVSASDHGIHLPDRELACAPINSE
                     VGQKYLGAMRAAINCALANRQIITHLVRQVFADILPQARLPLLYDVSHNTCKVEEHRV
                     DGASRKLYVHRKGATRAFGPGHPDIPASLRAFGQPVLIGGSMGAGSYILAGTSESESL
                     AFSSACHGAGRAMSRHQATRTWRGRRVVEELAARGILIRSPSMRGVAEEAPDAYKDVS
                     AVVDAADAAGLARKVARTEPLICVKG"
     gene            complement(26803..27240)
                     /locus_tag="PKN91_01915"
     CDS             complement(26803..27240)
                     /locus_tag="PKN91_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HNS26856.1"
                     /translation="MEAAGHPRLPRWEHFAHDADMGVRGLGSTLPEAFEQGALAMTAL
                     VTEPDAVEARERVTIECSAPDEELLFAEWLNSLVYEMATRHMVFGKFIVTIDGTRLSA
                     EAWGERIDAPRHQPAVEVKGATYTALRVASEASQWVAQTVVDV"
     gene            complement(27240..27650)
                     /locus_tag="PKN91_01920"
     CDS             complement(27240..27650)
                     /locus_tag="PKN91_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26857.1"
                     /translation="MPNELDPIVGQWYVYRDKGGMFRVVAADAAEDYIEIQSFDGDVE
                     ELDTIAWQAMDIETAEPPEDWTGPFDDIEPDDLGLSETGTTPQDWRQPLESVEAAEEP
                     WQDARSPDELEEEEEGRPPEVYAEEQETTRDRAR"
     gene            27904..28473
                     /locus_tag="PKN91_01925"
     CDS             27904..28473
                     /locus_tag="PKN91_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HNS26858.1"
                     /translation="MQTPLRITFRHMEPSPAVEARVREHVEWLERFYDRITGCHVVVE
                     RPPAHRHKGAPFDVKIDLTVPGDEIVVRSERAEHAAHVDVYVALRDVFDSARRLLQDH
                     ARRQRGDVKQHSLPTLGTIDQIEQGFGRIAADDGHYVYFHSNSVHGIPFDELTVGTVV
                     EFEEEQGDDGMQAAAVRLWGGAHESRKPA"
     gene            28501..29199
                     /locus_tag="PKN91_01930"
     CDS             28501..29199
                     /locus_tag="PKN91_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNS26859.1"
                     /translation="MNQHHGTQTTIALGTGVELKGFLEGSEDARGLIVFVHGSGSSRF
                     SARNNQVAQFLRGRGFATLLMDLLTTSEERVDAITRELRFDIPLLAERVTGTLDWLAR
                     HDRVGRLPIGLFGASTGAAAALIAAASQPTRVSAVVSRGGRPDLAADGLEAVQTPTIL
                     IVGSDDIDVLRLNQMAMARIPAETKLVTIEGATHLFEEPGTLDQVAQHAADWFERHLR
                     DRSAGGQGFPQSAS"
     gene            29284..29736
                     /locus_tag="PKN91_01935"
     CDS             29284..29736
                     /locus_tag="PKN91_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS26860.1"
                     /translation="MNERPTSFGTVGSICSREVIVATPGEPVRTALQLMQARRVGSVV
                     ICDDLSGRRIPVGIVTDRDIALSLLQRAPDVTVIPLRLVMSSDPLVLFEDQDVSEALA
                     ALRARGVRRAPVVDAARSLVGIVTIDDLLGLIARQLGDMARLIERQTL"
     gene            complement(29873..29949)
                     /locus_tag="PKN91_01940"
     tRNA            complement(29873..29949)
                     /locus_tag="PKN91_01940"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29913..29915),aa:Asp,seq:gtc)
     gene            complement(29953..30027)
                     /locus_tag="PKN91_01945"
     tRNA            complement(29953..30027)
                     /locus_tag="PKN91_01945"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29993..29995),aa:Val,seq:tac)
     gene            complement(30137..32578)
                     /gene="lon"
                     /locus_tag="PKN91_01950"
     CDS             complement(30137..32578)
                     /gene="lon"
                     /locus_tag="PKN91_01950"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNS26861.1"
                     /translation="MPESPPTTPKTTSLEKLPVLPLRDVVVYPHMVIPLFVGREKSIQ
                     ALDVAMKADKRIMLVAQKQADVDDPKVDDLYRIGTVATILQLLKLPDGTVKVLVEGVD
                     RAQIDRIHPGEFYACDVTLQPDSDVYDEKEMDVLGRSVISQFEQYVKLNKKVPPEVLT
                     ALAGIEQPGRLADTVAAHMALKLPEKQKVLEILDIKQRLEHILVAIEGEMDMLQIEKR
                     IRGRVKSQMEKSQREYYLNEQMKAIQKELGDMEDGANELAEIEQKIQKAGMPKEARDK
                     AMAELGKLKMMSPMSAEATVVRNYVDWLVKVPWKKRTKVIKDIARAEKVLDEDHYGLE
                     KVKERIVEYLAVQQRVDKLKGPILCLVGPPGVGKTSLGQSIARATNRKFVRMSLGGVR
                     DEAEIRGHRRTYIGSMPGKIVQNMAKTGVHNPLFLLDEVDKMSMDFRGDPSSALLEVL
                     DPEQNTAFNDHYLEVDFDLSQVMFVCTANSLNIPAPLLDRMEVIRLPGYTEDEKLNIA
                     RRYLVPKQMKNNGVKKDELKVTDEALLDIVRYYTREAGVRNLEREIAKICRKTVKQQL
                     LAKKDKAVTVTPKNLDKFLGVRRFRYGKAEDQSRIGQVTGLAWTEVGGELLTIEAAVV
                     PGKGKLQHTGQLGEVMQESIQAAMTVVRSRANVLGIEPDFYQKHDVHLHMPEGATPKD
                     GPSAGIGMCTALVSALTKIPVRSDVAMTGEITLRGEVLPIGGLKEKLLAAHRGGIETV
                     LIPIDNEKDLVEIPDNIKGNLRIKPVKWIDEVLEIALAARPVPIASAADAPAAADPKR
                     RRAARRAGKGGVQAH"
     gene            complement(32675..>33440)
                     /gene="clpX"
                     /locus_tag="PKN91_01955"
     CDS             complement(32675..>33440)
                     /gene="clpX"
                     /locus_tag="PKN91_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNS26862.1"
                     /translation="KAQTGIVYIDEIDKISRKADNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPQQEFLQVDTSNILFICGGAFSGLEKIILNRTQKGGIGFTSDVRDM
                     TAVDRPDVFHEVEPEDLIKYGLIPEFVGRLPVVATLEELDEAALVSILTEPKNALTKQ
                     YKRLFEMEGAELEFREEALKAVAQRAMQRKTGARGLRTILETILLDTMYELPSLRGVS
                     KVVIDETVIDGQTKPYIVYKSESAEEPPRRAHGIAS"
BASE COUNT         5532 a        11331 c        10931 g         5646 t
ORIGIN      
        1 aggagccgtc gaagcgcgcg ctctggtcgt cgctcgcatc cgtgacgccg gtgacctgca
       61 gcttggcggc gagccgatcg cccatccagc tcgcgcgcag ttcgagctct ccttcttcac
      121 ggggcgtgtc ggcgaaggtc cgcagcggat cgggatcgtg cgcccccgag acccggacgg
      181 cgaggccgtc gaggccgctc cttgcctcgc gacgggcggc gctctgtacg cgcatcaacg
      241 attgacgaag cgccgcgctc gcaccgtcgg gcaactgcgc ggcattgaca ctgcgggcca
      301 cgtccggcca cgacatgggc caggtggtga tcgggccgcg cagaatgccg gcatcggcca
      361 gcatctcgat atcgtggcgc atggcaggat cgcccggagc gagccaggga cctgcgtagg
      421 ccggcgggac gatgagcgtg gcgagcagcg ccgacaggcg cagaatccgg tcattattca
      481 ccacgggcaa atctactttc catgatcggg ccgtcaagat gtcgtaatat tctgacatca
      541 ttcgggcccc ggttcgggtt acccttcgca ccccatgcgc atcctgttcg tcccggtctc
      601 cgccaccact ggctctgggg agtatgtgcg gtcgctcgcg atcgccgcgg ccctgcgtgc
      661 aatgacgaac gccgagatct ccttcgtgct caaccggaat gcgccgcacg ccgccaccac
      721 acccttcccg gcgctgctgc tgccctcgtc cccgaccttc catccgcgcg aggtgtcggc
      781 gctgatacgc gaacggcggc cggatctcgt cgtgttcgac aacgccggac ggaccgcgca
      841 gttgagggcc gcgagagaga gtggcgcgcg ggtcgtctat atcagctcgc ggccccgcca
      901 gcggcgcaag gcattcaggc tgaagtggct gccgctgctc gacgaacact ggatcgcctg
      961 gcccgaattc atggccggtc cgctgagcgg cagcgagcgg ctcaagatgc gctacggcgc
     1021 gcgccgcccc acggtgcgct tcttcgacac gatcgtgcca acgccgaccg acgcggaatc
     1081 agcggctgtc gcagcccgct ttggtctcga gcccgaacag ttcgcgctcg tggtcccggg
     1141 cggcggcggc gcgcacccgg gtgcgaggca gggcccgcgc accatggcgg ccgccgcact
     1201 gcgcctcgct cgcaccggcc acccgactct tctggtcggg ccgcacggcg aggtcgccgc
     1261 tgacggatcg ggcctgcggt actcgccgca cctgccgatc caggatctcg cggcgctgat
     1321 ggcgcgcgca cgcgtggtgg ttgccaacgg tggcgacaca ctgctgcagg cgctgtcgtt
     1381 ccggcggcat tgcgtcgccg cgccccttgc ccacgatcag gtcgggcgca ttcgccactg
     1441 cgctgatgcg ggcatcgcca ggggcgtcga cctcgacgag gtggaggtcg cgcgcgcggc
     1501 cgaggaactg ctgaacgacg gctcgctgcg cgcggagcag gccgcgcgga tcgcgcagcg
     1561 gccgatccgc aatgaactcg aattcgcggt gggcgctctt gccagactgg ccgcttaact
     1621 gcccgctgcg cactgcgctc tgcgtggcgc tcctcgccgg cttcgtgggc atggcgcagg
     1681 cgcgcggtgt ctcgccgtac ctgccactca gtatcgagcc cgagatggag cggcagatcg
     1741 agcaggttct cacgctcgcc gacaaaccct tcctcacgcg cccgatcgcc gcggccgtgg
     1801 tctgggacgc cctgcccgcc gcctgtgcgc gtgatgaggc gctctgtcgc agcgtcgagc
     1861 gctacctgag gcgttacatg ggacgcgcag gcgttgccga agcctcgatc gccgccggtc
     1921 atgccgatgg cagcgcccgc cccgtgccga accaccacgg cctcgagacc gaaagtccgt
     1981 gggaagcctc ggcgcgcgtc tactggcagc ccttcgacca cgccctgctg acgcttggcg
     2041 gcgtggccta cgacggggaa gcgactccca ccggttcgct gctgagcctt ggctgggact
     2101 tcgcgcagct cgacatgggg tttcgcgacc actggctgtc accgttcacg gacagcgcca
     2161 tgctgctcgc caccgaggcg ccgacgatgc cctcggtcac gatttcgaac tacatgccgc
     2221 tcacccgcct cggcctccac tacgaagtgt tcgccgcgca gatgtccggc tcggacgcca
     2281 tcgttttcga gggcggcctc acgtcgggcg agcccaatgt cgcgggtctg cacctgtcga
     2341 tcgagccggc aagcggctgg tcgctcggtt tcaatcgcgt catgcagttc ggcggtggcg
     2401 atcgcgacga ctattccttc ggtgacgtca tcgacgcatt cttcaatccc tccagccatg
     2461 acaacgcgaa cgacccggaa gattccgagt tcggcaacca ggtcgcctcg atcacgagcc
     2521 gcttcgtgtt cccgggcgcc gtgccgtttt ccgtctacgc ggaatacgcg ggcgaggaca
     2581 cctcgcgcgg tcgcaactac ctgctcggca attccgccct gtcggtcggc atcgacttcc
     2641 cgctgctctg ggagcggttc gacctgacgt tcgagctgtc cgaatggcag aacggctggt
     2701 acgtacacca cgtatatggc gatggcctca cgaacgaagg ccgtgtactg ggccattggg
     2761 gcgcggacca gcgcgtgttc aacgatgatg tcggcgcgta cagcgccatg gcgcgcatcg
     2821 gttggcggcc cgcgttcggc ggcagctttg tgctgcgcgg ccgggtgatc gagaacgaat
     2881 cctactcggc ggcagcgtac gagcagggtt acgacctggc gttgagctat gcgcgcccgg
     2941 tcggagcatt ctcgctcggc ggcgaggttt acgcgggtcg cgacgttttc ggcgaggatt
     3001 tctcgcgtgt caccgcgttc gtgcgatacg cgccggaagg cgccgccggc atggggcaca
     3061 ccgtgatgtc gaccgcatcg ggccggcggg agcgcgcctc gggcgcggag ctgttcgtcg
     3121 atctcggcgt caacgccaac caggtgcgca tcgatctcga tgacgacctg ccgagggcga
     3181 cgagcgacac tgaaatcgcc ttgcacctcg gcgtaggcgc cagacgccag gtcaccgatc
     3241 gccaggacct cggcgtgcgc ctcgagtacg acgagatcga cggccatgca ctgatcggcg
     3301 tgcgcgcgct cgattaccgt taccgcatca agggaccctt cgcgctcacc ggcttcgcgg
     3361 gtgccgcgcg ctatgacctc gcgacgcccg cctatgggct ctacatcggc gcaggcgcgc
     3421 aatggcgcgg cgtgctgcga tcggacttcg atctctcgct cgacctgcgc tacgcatcga
     3481 aggtggcgcg tgacgacctc gtcgcggacg atccggtcgg cggccgcccc gacagcttct
     3541 acgacatcgc gagtgcgacg ctctatctga gctggcgatt ctgagcggca gcggccatgg
     3601 cgcccgccgc gctggtcaca cgccgtagcg ggcgcagagc acttcgaacg gcgaatcggc
     3661 ggcgaaacgc gcgtccttat cgaagccagg cgagcgggcg gccatgttgt tccacaggtg
     3721 caccgcgcgg ctttctgcgc cccagggcaa cgcgccgtcc ggggcgggct cgaagagcgt
     3781 tcgccaatgt tgcgcgggca cgggatagaa gtgtgcgctc ggcagcgcgc gctcgaggca
     3841 gcccaggtgg cgggccgcgg cggtcaggcc cttgggcccc agttcgcccc agcgcagccg
     3901 atcgcgccga tcgccctgca gatagcgacg gcgcagcttg cgcagtcgca tccgccaccc
     3961 gtcatagggt tggatgcgat tcgggtgctc gcagcagtcc gcaagccagc gcgcgagcgc
     4021 gtggcctgcc ggcaagccga ggatcgcgtt gttgacctgg tgcgcatcct cccatgcgaa
     4081 gaccacggcc tgcggatacg cgatcggctg caggcatacg acatccatgt cggcccagat
     4141 gccgccacgc gcggcaagca gccggtaacg gaaccagtca gcgaacagcg ccaccgagcc
     4201 ggtgcgccga tgatggaaca acgcatcgcg cggcaggacc tctgcggcgt cgcgcagctg
     4261 cacgccggcc ggcacgccgc gcggctcgtc atagacatag agctcgacgg cgtggcggtg
     4321 gtgcaggaag gacgcgaccg cgagccgctc gaggcgcgac agcggcgggc cgtgccagaa
     4381 catgtggatg gtggtcaggg tcactgcgtt cggctcgtgg ttcccgagta tggcgttcgg
     4441 gggcgtccat accctccccc tcgcacccgt atcaactccc ggtcagattc agaatcactg
     4501 cttccagccg cgcagcgtgg cgagcagtgg acgcaggtgc tccggcgcag ccgcgaggcc
     4561 cgcatcgctg ccagagccgg gcctgccctc ccccgtcacc gcgggcacca ggcctgcgca
     4621 cagcttcttg ccgagttcca cgccccactg gtcgaacgcg ttgatgcccc agatcacgct
     4681 ttgcgcgaac accttgtgtt cgtagtgcgc gatcagccgg ccgagggtcg ccggatcgag
     4741 cgcatcaaag gcgagcagcg atagcggcct attgcccccg tgcaccttgt gcaccgagcc
     4801 cgccacctcg agcggctggc cgaacgcgaa ggcctcggcc tgcgcgaggc agttcgcgat
     4861 cgccagatcg tgctggccct ggcgcccgca ggagctgcgc gccggcagca ggaaatcgag
     4921 cgcggcgcgc ggcgtaccct ggtgcagaag ctggaagaag gaatgctgcg cattgttgcc
     4981 gggctcgccc cagatcacag gtcccgtggt ccactgcacg gcggtgccgc cctgcgtcac
     5041 cgacttgccg agactctcca tttcgagctg ctgcaggtag gcgggaaaac gcgcgaggcg
     5101 ctggtcgtac ggcagcaccg cgagcgtggg cagcgcaagg aagttgccgt tccacacgcc
     5161 gagcaacgcc gcgagcgccg gcagattcgt gtgccacggc gccgaggcga agtgctcgtc
     5221 gatgtcccgg ccgccgtcga ggaacgccag gaagcgctcg cggccgatcg cgatcgcgag
     5281 tgagacgccg atcgacgacc agatggagta tcggccgccg acccagtccc acatctcgaa
     5341 gcgatagtcg gggtgcacgc cgaagtcatt catcgccgcg gtgttgaccg acacggccgc
     5401 gaaatgatcc ggcaccgcgg cctcgcccag ggtgcccgcg atccactcgc gcgccgcgcg
     5461 ggcattcgcc agcgtttcct gcgtcgtgaa ggttttcgag gcgacgatga acagagtcgt
     5521 ggcgggatcg gctgtggcga tgacatccgc gatctggcag ccgtcgacgt tcgagacgaa
     5581 ctcgagtcgg ggcgccccgt cggtgtagcg ctgcagcgcc tgcaccgcca tcgctggccc
     5641 caggtcggag cccccgatgc cgatgttgac gaccgtcctg tacctggccc gcatgcgctc
     5701 ggcgaaatcg agcatgcgtg cgcgctccgc gagcaccagc cgctcgacct gtgcgccgcc
     5761 gagttgctga ccctcgcgct ggcgcagcgc gacatgcagc accgcgcgct tctcggtcgt
     5821 gttgatctgc tcgccgcggc gcatggccgc gatcgactcg cgcagcccca ccgcctcggc
     5881 cagcgccgcg agcgtggcga gcacgtcggt atcgacgcgc tggcgtgaat agtcgagcag
     5941 caggttggcc gtgcgcgccg agaactgctc gaatcgcttc gcgtcggccg cgaacagctc
     6001 gcgcagatgg cgcgcgccgc tcgtcgaggt gagctcgcgc agccgctgcc agggccgggt
     6061 ttcggggccc gccacgcgcg gcggctgcgg agcctgcgcg ctcaccggta gaaggccgcg
     6121 atgcggtcca ccacgaactg caactgcgct gtctcgagtt cgggaaagat cggcagcgcc
     6181 agcgtgtcgg cggccgcacg ctcggactgc gggaaatcgc ccgctccgtg cccgaggtag
     6241 gcgaaacact cctgcaggtg cagcggcacc gggtaataga tctcggtccc gatgtcgctc
     6301 gcggcgagat gcgagcgcag cgcgtcgcga tcgcgcacgc ggatcacgta ctggttgtag
     6361 atgtgccggg cgccaggcgc ggccggcggg gtgaccaccg catcgccgag tgcggccctg
     6421 gcgaaggccg cgtcgtagta cgccgcgttc ctctgccgcc cggcggtcca ggcatccagg
     6481 tgcggcagct tcaccccgag caccgccgcc tgcaactcgt cgatgcggaa gttgccaccg
     6541 atgaacgcgt ggtagtactt cggctccccg ccgtgcacgc ggaagatgcg cagccgctcg
     6601 gcgagcgccg catcgttcgc cacgcacatg ccggcatcgc cgaacgcgcc gaggttcttg
     6661 gtcggaaaga acgacaggca gccgatgtcg ccgaacgaac acgcgcggcg gccctgcgca
     6721 tcggccgcgc cgatcgcctg cgccgcgtcc tcgatcacgc gcaacccgtg cttgcgggcg
     6781 agcgccatca gcggatccat gtcggcgacc tggccgtaga ggtgcaccgg catcagcgcc
     6841 ttgatgacac ccttcgtccg cttgttgacc agcttgccgt cccgcgactc gcattcgcgc
     6901 tcgatgaaat gcgaaaccgc ctgcggcgag aggttgaacg acccgggttc gatgtcgcag
     6961 aacacgggcc gcgcgccgac gcgcgcgatc gtgcccgccg tcgcgaagaa ggtgtagggc
     7021 gtcgtgatca cctcgtcgcc cggcccgatg tcgagcacca tcagcgcgac gagcagcgca
     7081 tcggtgcccg aggacacgcc gatgccgaac tgcgtgccgg tgtacgcggc gacgtccgcc
     7141 tcgagcttct tcaccgcggg gccgaggatg aaatactggg attcgctcac cgcctcgatg
     7201 gcggcgtcga gttcgcgctt cagtgcgcgg tactgcggct tcaggtctag cagggggact
     7261 ttcatgcccc caagtctagc cgagattggt gacgcgggat tcggccggcg ggccgccttt
     7321 gtagtactcg aggtaccagt cgatgaagcg gcgcaccccg gtttcgatcg gcgtgtcggg
     7381 cttgtagccc acgtcctcga ccagatcctg cacgtcggcc tcggtatccg gcacgtcgcc
     7441 cggctgcagc ggcagcaggt tcatttccgc cttgcgaccc aggcactcct cgatcacctg
     7501 gatatagcgc cgcaactcca cgggccggtt attgccgatg ttgtacagcc ggaacggcgc
     7561 gctgctggtc gccgggtcgg ggtgtgcgct gtcccaggcg ggattcggct gcgcgatgcg
     7621 gtcggtcgcg cgcaccacgc cctcgacgat gtcgtcgacg aaagtgaagt cgcgccggtg
     7681 atggccgtgg ttgaagacat cgatcggctt gccggcaagg atgttcttcg cgaacaggaa
     7741 cagcgccatg tcgggccgcc cccagggacc gtagaccgtg aagaatcgca acccggtgac
     7801 cggcagcccg aacagccacg cgtagttgtg cgccatcagc tcattggcgc gcttggtcgt
     7861 ggcatacatg gacagcgggt gcccgacctc gctgtgcacg ctgaagggca tgtcggtgtg
     7921 cgcgccgtac accgagctgg tcgaagcgaa caccaggtgc tcgaccgcgt tgtgccggca
     7981 gccctccagc acgttcagcg tgccggtcac gttgctcgac acgtaggcat gcggattctc
     8041 gagcgaatag cgcaccccgg cctgcgcggc gaggtgcacg acgcgcttga acttctcacg
     8101 cttgaacagg tgcgtcatgc cgccctggtc ggccaggtcg agcttgacga agcgaaagcc
     8161 cggctgcggc tcgagccgcg cgaggcgcgc gcgcttcagg ttcacgtcat agtagtcgtt
     8221 caggttgtcg aggccgacca cctcgtcgcc gcgcgcgagc aggcgctgcg cgacatgcga
     8281 gccgatgaac cctgcggcgc cggtgacgag gaccttcatc ggggcgtcac aggcgcccgt
     8341 cgacctggtc ggccgggaac acgtacttga tgtcgtagac caccgaattc ctgcggccga
     8401 agcgacgcac cgcggccggc cccatgtcct tgaactgctg gtgcccgacc gcgagcacca
     8461 cggcgtcgta ggcgcccttc ttcggtgcct tgaccggcct gatgccgtat tcgtgcttcg
     8521 cctcgcgcgg atcgacccac gggtcgtaca cgtcgacctt ggcgccgaac ttgccgagtt
     8581 cgcgcaccac gtcggcgacc ttggaattgc gcaggtcggg gcagttctcc ttgaacgcga
     8641 gcccgagcac cagcacgcgc gagcccttca cgtggatgcg ccggtcgctc atcagccgcg
     8701 ccacctcgcc ggcgacatac agcgccatgt tgtcgttcag gcgccggccc gcgaggatca
     8761 tctcggggtg gtagccgatc tcctgcgcct tgtgtgtcag gtaataggga tcgacgccga
     8821 tgcagtggcc gccgaccaga cccggtctga acggcaggaa gttccacttg gtgccggagg
     8881 cgagcagcac ctcctcggtg tcgatgccga ggcggttgaa gatcagcgcg agttcgttga
     8941 tcaacgcgat gttgacgtcg cgctgcgtgt tctcgatcac cttggcggct tcggcgacgc
     9001 ggatgctcga ggccttgtgc gtgccggcgg tgatgatcga ggcgtagagc ttgtcgacga
     9061 attcggcgac ctcgggtgtc gagccggaag tcaccttctt gatggtcgtg acccgatgct
     9121 gcttgtcgcc cgggttgatg cgctcggggc tgtagcccgc aaagaagtcc ttgttgaact
     9181 tgagccccga gacgcgctcg agcaccggca cgcactcttc ctccgtgcat cccgggtaca
     9241 cggtcgattc gtacaccacc acgtcgccct tcttgagcac cttgccgatc gtctcgctcg
     9301 ccttgacgag cggtgtcagg tcggggcgct tgtagtcgtc gatcggtgtc ggcaccgtga
     9361 cgatgaacac gcggcacttg cgcagcgtcg cgaggtcgga cgtgaagctg aggcgcttcg
     9421 aggacttgag ttccgcggcg cttacctcga gggtgctgtc gcgccccctc gacagctcgg
     9481 cgatgcgctc gggcttgatg tcgaagccga cggtttcgta gtgcttgcca aactcgaccg
     9541 ccagcggcag acccacatag ccgagaccga ccacaccgat acgacacttg cgcaagctga
     9601 ccatttggcc ctcggatcct ggctgaaaac gcgattattg gcggtttgcg gctgtccgga
     9661 cgcgatccac gtcgcattgc cggaacacgc agcggtcccc gctcgcccgg gcgatcaggt
     9721 cgagcatgcg ctcgtgatcg gcgagttctg cggcggtggc ctggcggacg acgaggccgg
     9781 cggccggacg caccagcgcc tgtacttccc cgccctggct gcccggcgcg ccgggagtgt
     9841 gctgcgagtc gagggcgagc gtgctctgcc cgccggtcat cgtcaggtat acgtcggcga
     9901 ggatgcgcgc gtcgagcagc gcgccgtgca gttcgcggtg cgaattgtcg atgccgtagc
     9961 gcctgcacag tgcatcgaga ctgttgcgct ggcccggatg catttcgcgc gcgagcgcga
    10021 gcgtatcgag cacaccgcac aggtctccga tccgtcgcgg caggccgctc gactcgggca
    10081 gtcgctgcag ctccgcctcg aggaaaccca cgtcgaacga cgcattgtgg atcacgagtt
    10141 ctgcgccgga gatgaattcg atcagttcgg ccgcgacctg tgcgaaacgc ggtgcgtcgg
    10201 ccagatcggc gctcgagatg ccgtgcacgg ctctcgcgcc ctcgtcgatg gcgcgctcgg
    10261 gattcaggta gcgatggaag ttgcggccgg tggcgcgccg gttgcgcaat tccaccgcgc
    10321 cgatctcgat gatgcgatgg ccgcgttcga cttcgaggcc cgtggtttcg gtatcgagga
    10381 cgatctggcg catcgcgcgc aattctactc gatgccgtcg cgcatgagcg catcgatcgc
    10441 gaggttcgcg agccggtcga cgtgctcgtt gccggggacg ccggaatgtg ccttcaccca
    10501 atgccacctg accttgtggc gggcctgcgc ggcatcgagc gcctcccaga gatcctggtt
    10561 cttcaccggc ttcctcgcgg cggtcttcca gccgcgcgcc ttccagccgg gcagccactc
    10621 gctgatgccg cgcttgacgt actccgaatc cgtatgcaga tcgacgaccg acgggcgctt
    10681 cagggcctcg aacgcgcgga tcaccgcggt gagctccatg cggttgttgg tcgtcaggcg
    10741 ctcggcgccc gacagctcgc gccggtgcgc gccgctgctc agcagcgcgg cccagccgcc
    10801 gggtcccggg ttgccgcgac aggcaccgtc ggtgtagatg gtcaccgcgc tcatgagccg
    10861 gaacgcgacg tcggcttcac caggctgccg atgaccgcgg aacgctcacg gaaacggggc
    10921 ctgatcggtg tcatcgtgta gacacgcttg cgggcccgga gcaggtacac gccgtggtgg
    10981 aagtggcgcg aggcgacgat ctcgtagccg agcagcgcca gccagtcgga cacgcgccgt
    11041 tcggcgagca gacccttgag gcccggcggg aaactgcgcc gcgccgcgcg cgcgcgcagg
    11101 ccccacaggc cgaacgggcg aaacccgagg acgatcagct gcccctcgcc gaccagcacc
    11161 cggtcgacct cgcggatcat cgtcttcgga tccggcagga actcgagcgt gtggggcagc
    11221 agcaccgcat cgacggtacc gctcgcgatc ggcagctgcg gcaggcgcgc gaccacatcg
    11281 acacccggcg cctcgcgctc gacgaccacc gtttgctggc gcgtgcgcgc ggagcggatc
    11341 aggccgcgcg ggggccccca cgccccgatc tgcagcaatt cccagccgaa cacgtcagcg
    11401 agcgcctcgg cgagcagcct cgattcggcc tcgagcaacg ccgccccggg ggtgccgcgc
    11461 agccaggaat cagggcaagt agcagcagga gtcattcggc gaggcccggt tggtgtgtga
    11521 acagcgacac aaaaatgcta gccaatatgt gaacttacag gttaaatacc cggattcaag
    11581 ggggtacgtt aacgaagctg ccgcctctcc agacggggga cgatacttga agcgattgct
    11641 gggccttgtg gccgcactga cattgagtgc ctgcggcact ttacgtgaga ccccgccacc
    11701 ggtgccggcg cccgaaccgg ttgcggtggt gcctgagccc gagccgctcg ttgcccccgc
    11761 gcccgaagtc ccggtcaccg tcatcggcat cacgcccgag aagtactcgg acctcttcga
    11821 ccgcatccgc gccggctacc agctcgagga cgtcgactcg cgccaggtcg accgcgagtt
    11881 ccgcttctac gccaacaacc cggactacct cggccgcgtg ttcaatcgcg cggcgccgta
    11941 cctgtaccac atcgtccagg agatcgaggc gcgacagatg ccgctcgagc tcgccctgct
    12001 gccggtggtg gaaagcgcat tcgagcccta tgcctactcg gtcgcccgtg cctcgggcct
    12061 gtggcagttc attcccccga ccggatcgcg ctacgggctg aagcagaact ggtggtatga
    12121 cgggcgccgg gacgtggtcg aatccacccg cgccgcgctc gactacctgc agttcctgca
    12181 ctccgaattc gacgattggc tgctggcggt cgccgcctac aactgcggcg aagccaacgt
    12241 ggcgcgcgcc atccgcgcca acaaggccgc gggcaagccc accgatttct tctcgatcag
    12301 cgcgcgcctg ccgaaggaaa cccgcgccta cgtgccgaag ctgctcggga tgtcgcgcct
    12361 gttcctctcg ccgcaggact acggcatcga gttcagcagc ctgccgaacg agccctattt
    12421 cgcacaggtc gagacgctcg gccagatcga catgaagctc gccgcggaac tcgcgggcat
    12481 ctcctacgaa gagctctata cgctgaatcc cgcattccac cgctgggcga ccgacccggc
    12541 cggcccgcac tacctgctgg tgccggccga caccgccgat ctcttccgcg agagcgtccg
    12601 gcagctcacc gccgacgagc gcatgcgtgt ggcgcaccat cgcgtgcagc ctggcgacac
    12661 cgtcgcggac atcgcgcgcc gctaccacac gcagacccac gtgatccgcg aactgaacga
    12721 cctcaccgcg gacgccaagc tgatggtcgg tgacgacctg cgcgtgccct cgggcgtgac
    12781 cgagctgccc gcgaaggtgc agctcgccgc gctgcgcgtc gatcgcggca cccgcgccag
    12841 gggcggcgtg catgtcgtga ggcgcggcga cacgctctcg gccatcgcac gccgcaaccg
    12901 cctgagcgtg cgagcgctcg ccgcgatgaa caatatcggc gtcaacgaca cgatccgcgc
    12961 cggccagcgc ctcgtcgtca acaagtcggc agcggcaagc cgcgccgccc cgacgcgcac
    13021 cgcctcggcc aaccacggcg ccggcgccgg cagcggcggc tcgagctaca cggtgcgcaa
    13081 gggtgacagc ctctggagca tcgcgcgccg cttcggcgtg agtgtctcgc agatcgtcgc
    13141 gtggaacggc atctccagcg aatcgacgat caagccggga cagaaactga ccgtcaaaaa
    13201 agtacccggg taagacatgg gattcatggc tggcaagcgc gcgctcatcg tgggtgtcgc
    13261 gaccgaacgt tccatcgcat ggggcatcgc gcaggcgatg caccgcgaag gcgccgaact
    13321 cgcaatctcc tacgcgaacg acaaacttcg ggagcgcgtg gtgccgctgg cggccgaact
    13381 cggcgcgctc acgctgccgc ttgacgtcac caacgacgcg gagatcgacg gcgcctttga
    13441 tctgctgaag cgtgaatggg gcagcctcga cgtgctcgtg cacgcggtcg cctacgcgcc
    13501 ccgtgaagcg ctcaccggcg gcttcaccga ggcgacttcg cgcgagtcgt ttcgcatcgc
    13561 ccacgacatc tcgagctaca gcctgaccgc gctcgcccgc ggcgcggcac cgctcatggc
    13621 gggccgcaac ggcgcgattg tcaccctcag ctacctcggc gcggtgcgct cggtgccggg
    13681 ctacaacgtc atgggagtcg cgaaggcaag cctcgaggcg agcgtgcgtt tcctggccgc
    13741 cgacctcggc ccgcagggca tccgcgtcaa cgccatctcc gccggcccta tcaagacgct
    13801 cgccgcggcg ggcatccccg gctttcgcaa gatgctcggt cgcgtcgccg aagcggcgcc
    13861 gctgcggcgc aatgtcacgc tcgacgacgt cggcaacagc gcggccttcc tgtgctcgga
    13921 cctcgcggcc gggatcaccg gcgagatcct gtacgtggac agcggctaca gtacggtggg
    13981 gatgagcttt ccgctcgaaa ccgacggctg agccgccgcg ggtcctactc gaacgccttc
    14041 ggattcaccg acaccttcgc gttctgccgc gcctcggcga cataggcgat gacgtcggcc
    14101 gagccgacac gtgcgacgat ctgctgcagg cgctcctggc gcgcctccgg gctgagcggc
    14161 ggcacgtcgc tcttcacacc gagcagcgcg acgacagccg cgccgtcggc ggtcggcacg
    14221 accgactgga tcggctcacc atcgggatgc ggcatgctga atacgctggc gcgaatctcg
    14281 gcgggtaccg aagggtcgat gcgcgcaatc tggcgtgact cggcgtcgag ctgctcgccc
    14341 ttgcgcagcc gcgcgagcgc ggcatcggcg gccgcacgcg ccgcggccat gccctgttca
    14401 tgcttcagtg tcgacacgat gctcgcctgc acctccgcaa gcggccgggc ctgcggcgcg
    14461 cggtgttcga gcacctgcac gacgatcagg cgatcctcgc cgagcccgac cggcccgccg
    14521 acacgtccct ggttgaggac ggtgtcgctg aacacgatgt cctcgagcgc cggatcggtg
    14581 ccgagcgtgc cgccgccgct gccgcgctcg tacacttcga ccttgccggt cgagaggctg
    14641 aattcctgca ccagggcgtc cagcgtgaca cccggttgct cagtccgggc ctgcagctgc
    14701 tcctgcgcgt cgccgaaccg gtcggccgcc tcgtcgcgcc ggtactgggc gtcgagttcg
    14761 gagcgcgctt cctcgaaggt cttcgacttg ccgggctcga tcgcttcgag cctgatcagg
    14821 tggtagccga actgggtctt gaccggacca cgggtctcac cgacctgcat gccgaacagc
    14881 gcatcggcga acggcgcgac gaaagtctcg cgcgtggcgt agcccagatc gccgccgttg
    14941 gcggcggaga ccgagtcttt cgaattcgcg gcagcgagcg cggcgaagtc gccgcccgcg
    15001 ttcagctgcg cgagcaggtc ctgggcggtc ttttcatcgt cgacgaggat gtggcgtgcg
    15061 cggcgggttt caggggtcgt gtagcggtcc ctgttttccg cgtacagccg gcgaaggtcg
    15121 tcatcgacga ccgtgatctg cgtcgccacc tggtcgagcc gcagttcacc gtacgcgagg
    15181 cgcaccgact cggtggtcat gaacttcgcg gtgttggcct cgtagtaggc gcgcacggcg
    15241 tcgtcgccga ccgccgcggg tgcgtaatcg ctggccttga acagtgcata acgcacttcg
    15301 cgctgctcgt cctcgagcgc gatgatgcgg tcgacttccg cgggcgtgac gaaatcggac
    15361 agcgcgatcg cgcgctgcaa ctgctggcgt gcgagcgagg tacgcaggtc cgcctcgaag
    15421 ccggcgaccg tgagccccac ctgcgcgagg cgcgccttcg caacgctcgc gtcgtacttg
    15481 ccgtccacct ggaaggccgg ctcctgctgg atggccgtct tgatctgctc gtcggagacg
    15541 cgatagccga ggtcgtggag gcgggcatcg agcatcgcgc gggtaatgag gccctcgagg
    15601 acgctctgct gaagctgcgc cttgctgtcc tcgggaattt cgccgccgaa cgcctgcacg
    15661 gcctgcgcct gctgctcgag ccaggcctgg cgtgccgtct ccagcggaat cttctcgccg
    15721 ttcaccttcg cggcataggc actctgcccg atgccgacat cgacgatgcc gtacgcgccc
    15781 caggcgacga atacgagcga gatcgccccc aggatgaagt acgcaagcgg gccgttgaga
    15841 ttttcccgga tcctctgaag catcgactgg ccttgtgtat tgaggtggcg gagcggacgg
    15901 ggttggggcc cgccaaaaat caatataaat cagtaattta ctacgatgtc acctgggacc
    15961 cgacgtgcca tgatctcccg tggaagcccc gcaacacgcc cgcggccggc gcggattgta
    16021 cgggggtcac gaccctcatg gaaggcgtcg atccctcggc gggctccgag cgaagcgcaa
    16081 tcccatcgtg aggctgatag aggccgaccc gcggattccc ccctacatcc gcgtagatcc
    16141 cccaagtgcg cggcgcagtg aacggcgcat gctttttgcc ctcgcaggga gaattcgcca
    16201 tgcaccccac gactgccgaa ctgtttacca agacgctgga gaagacgggt cgctggatcg
    16261 atgatctgat gaccgagatg ggcacgtcgg acccgcgcca ggcctattca gtgctgcgtg
    16321 ccgtgctgca cgcattgcgt gaccgattga ccgtagacga agccgtcaat ctcggcgctc
    16381 aattgccaat gctcgtgcgc ggtttctact acgacggctg gcggcccgaa gggcgcccgg
    16441 acaggtatcg tcacaagtcc gaattcctcc tccgtgtcgc gaatgcatac ggggggctgt
    16501 cggccaccga acaggaaccc gcagtgggag cagtgttccg cctcctctca cgccacgtga
    16561 ccgaaggaga gatacgacac gtgcgcgatc agctgcccgc ggaagtgcgc gacctgtggg
    16621 cgccgagcgc ctgaggcgat cagctgagat cgtgtgatcg tcaccgacgt cgcaggagtg
    16681 tcatccgcac tgaccagata gagccggctc gcaagtgctg cgtgcatttt cgtcatggca
    16741 gccgtgctcg ggctcggcgc gacaccagtc tgcgccttca gcgacgatga ggtgactcgc
    16801 ggaagccggc atagggcgac gcagcaatac gcagtatcgc gaatgaacgc cgtggaattc
    16861 cgggtgttcc aggcgcagct gcggacgcaa gctccgacaa gtccgccact gcgaccgcca
    16921 gtacggtcac ggcagcggct gctgcgccac cgagtcatca gcgccgctac gccacccttc
    16981 cccgaggaga tcagccatgc gtgtaaatga gatcatgaca aagcaagtga gctgctgcag
    17041 cgccgataac acgctcgccc aggcagcaca gctcatgtgg gatcacgatt gtggatgttt
    17101 gccggtctat tcgggcgacg gggcgagccg cctcgccggt gtcatcacgg atcgcgacat
    17161 ctgcatgtcg gcgctattca agggcaagcc gctgcacgag ctgcacgtgt ccgatgcgat
    17221 gtcccggcag cttcagacct gccgtccggg cgattcgctc gtcgacgccg aaagaacgat
    17281 gcgtcaagcg cgcattcgca ggctcccggt cgtcgatgac gaagggtcac tcgtcggcat
    17341 ggtttcgctc gccgatcttg cgcgcgaggc ggctcgggaa aaatccttgt cgaggcagga
    17401 gatcaccgag acggaggtgg gcgatacgct cgctgcgatc tgccagcccc aggcccagag
    17461 acaactggcc gcctgaatga accgggtttc gcccggtgcg accgggcgaa acccgtcggc
    17521 ggtcagctgc gaatgcatga cgcatgcgag cgccggctca atgcccaggc ccgtcggcgt
    17581 gtccgagctc atgaaagagc tctttcatcg cgcgcccgtt gcccgattct gcttgccgct
    17641 ttgccgtgct ccctggagca cgactgcgca tcgagatcca ggttcgagtg atggggagct
    17701 acagcaatgc cagttcacaa cgaagacctg gctgcggcct tcgatgaaat ggcggacctg
    17761 ctggcgctga aggcggagaa ccggtttcga atcaatgcct atcgccgggc cgcgcaagtg
    17821 gtgcgcgccc tgcccgagga gctcccgatc aagatcgctg cgggcttcga cccggacagc
    17881 ctgcctggca ttggcgcgga cctcgccgac aagattcgtg cattcgtgaa gaccggcgag
    17941 ctgcccggcc tggccgcgct ccgccgctcc gttccagcgg ggctccggga gatcatgaac
    18001 atccggaaca tcggccccag gcgcgcggtc gccttgtacg acgcgctcgg cgtccatgac
    18061 ctcgagacgc tacggagggc gcttgaaaag caccgcgtgc gtgaagtccg agggttcgga
    18121 gcaaagatgg aagagcgctt gaccaaggcg gtcgaggaga cgccggggcg cagtggtcgc
    18181 ctgctcaggt cagttgccgc gcagtacgcc accagcctcg agtcctacct gcgcgccctg
    18241 cctggcgtga cgaaggtcga cgtcgccggc agcttccggc gcggccgcga aacggtggga
    18301 gatctggact tcctcgtctg ttccgagcac ccgctggatg tcggcggcgc attggcgaag
    18361 tatgacgaaa caaccacgct cctggcgaaa gggccgacgc gttctgccgc ggtgttgaag
    18421 agtggcttgc aggtggatgt tcgcctcgtg ccatcggaaa gctatggcgc ggcgctctac
    18481 tacttcacag gcagcaaggc gcacaacgtc cacgtacgcc ggctcgccat ggcgcgcggc
    18541 ctgaagctga acgagtacgg cgtctttcgc ggtcgcaagc gcattgcggg cgatactgaa
    18601 gcatcggtgt tcgcatcgat cgccctgccc tgcatcgagc ccgaactgcg tgaggatcga
    18661 ggcgagatcg aggccgccca ggcgcgagcc ctgccgacct tgatcacgcg tcgcgacctg
    18721 cggggtgacc tccacgtcca tacgaaggcc acggatggta cggccagcct ggaggcgatg
    18781 gccgaggcgg cccggaaggc tggcctcgag tacatcgcga tcaccgatca ctccaggcac
    18841 ctcggcatgc tgcgcggcct ttcggcggac cggcttgccc gtcagctgaa cgagatcgat
    18901 gtgctcaacg catcgctcga cggcctcacg ctgctgaaag ggattgaagt ggacatcctg
    18961 gaggacggct cgcttgcgct acccgatagt gtgctcaaga gactcgatgt cgtcgtggca
    19021 tcgttgcacg atcacttcgg cctcagccgg cgaaggcaga cggaccggct gcttcgagtc
    19081 atcgatcgcc cgtatgtgtc ggtgcttggt caccccatgt cccgcctgat cggggagcgc
    19141 gcggccatcg aatgcgattg ggcacggctg ttcaggcgcg cgggcgagcg gccgatctac
    19201 ctggagttga actcccagcc cgcgcgcctc gacctggacg atgtgctggt tcgcgaggcg
    19261 gcggcgaacg acgtgctgat cagcatcgcc agtgacgcgc acggcatcgg cagcttcgac
    19321 ctcctcggcg gcggcgtgct gcaggcgcgc cgcggctggc tgtccaaggg ccaggtcctg
    19381 aacactcgcc cactgcagga gcttcgtcgc ctgttgcgca ggacggttct cgcatgagag
    19441 atcgccctcc gggactgccg agtgtcgtcg acgcagccgg cccaaaatgg tggaaacact
    19501 ggatctgccg gtttgatcgt cattagactc acactgccag tggagagacc gggaacgccc
    19561 gacgagcggt tcacgccgtt ggtggaatga gaagcaaccg acaggaaata cacccgtgtg
    19621 ggcaagctga agatctccgt ctgggatgtg ctgctcctgc ttggtgcggt catggcgatg
    19681 tactcgctca cgaccctgcg cacgaccgca acattgccgt acagcgagtt caaggccctt
    19741 gtggccgcgg gacgcgtggc agaggtcacg ctgggcgatc agcagatcgc aggcaggatc
    19801 aaggccggcc cactcgaaga cgtgctgtcg ccgcagagcc tcgccaagct ggtaccggac
    19861 agcgagggcg aacgcgcgtt cacgaccata gccgtcaatg acccggacct cgtgcagcag
    19921 ttggacgccg cgcatgttca atattcgggc gggcgcagtg cgaactggct gatgaccctg
    19981 ctctcctggg tgctgccggc gctgatcttc gtgggtctct ggagcttcat gctcaagcgc
    20041 atggcaggcg gcctgggcgc gagcagcgga ctgctcgatg tcggcaagag caaggcgcgg
    20101 ctctacatgg agaagctcgt gaacgtcacc ttcgcggatg tcgcgggcgt ggacgaagcg
    20161 aaggaggaat tgcaggagat catcaccttc ctgcgcgatc cgaagcagta tggccgcctc
    20221 ggggcgcgca tccccaaggg cgttctgctg gtgggaccgc cgggaaccgg caagaccttg
    20281 ctcgcgcgcg ccgtggccgg cgaggccagc gtgcccttct tctccatcag cggctcggaa
    20341 ttcgtggaaa tgttcgtcgg cgtgggcgcg gcgcgcgtgc gtgacctgtt cacgcaggcg
    20401 cgcgcgaagg cgccctgcat cattttcatc gatgagctcg atgcgctcgg acgcgcacgc
    20461 ggcttcggtc ccgccggcgg gggacacgac gagaaggagc agacgctgaa tcagctgctc
    20521 gcggagctcg atggattcga cccgagctcg ggcgtcgtgc tgctcgccgc caccaaccgt
    20581 cccgaaatcc tcgatccggc attgctgcgc gccgggcgct tcgaccgaca gatcctggtc
    20641 gaccgacccg acaagaccgg ccgcgtgcag atcctcaatg tgcacctgcg caagattcgc
    20701 atcgacacga gcgtcaaggt cgaggagatc gccgccatga ctcccggatt cacgggcgca
    20761 gacctcgcca atctggtgaa cgaagcggca ctcctcgcca cccgccgcaa cgccgatctc
    20821 gtcgcgatgc aggacatgtc gaatgccctg gagcgcatcg ttgccggcct cgaaaaacgc
    20881 aaccggctgc tcaatgaaac cgagcgtcgc accgtggcct atcacgagtg cggccatgcc
    20941 atcgtctcca tgctcacacc gagtgccgat cgggtccaga aggtgtccat catcccgcgt
    21001 ggcatcggcg cgcttggcta tacgttgcag cgcccgctcg aagaccgctt cctcatggcg
    21061 cggcacgagt tgatcgagcg catgatcgtc ctgctcggcg gccgcgccgc cgaacagctt
    21121 ctatgctcgg aagtctccac cggcgccgcg gacgacctgg taaaggccac cgacatcgcc
    21181 agacacatgg tcaagcgcta cggcatgagc gaggcgctgg gtccggtcag ctacgacagt
    21241 gcagctacac cgctcctggg tcccgcggcg gattactacc gtccacgcga ctacgccgaa
    21301 gccaccgccg gccgcatcga tacggaggtg cgcgagctga ttgaagaggc ggcgcggcgc
    21361 gcacacgagt tgctgcttgc gcatcgcggc acactcgaat cgatggcggc cgagctgctc
    21421 gcacgcgaga cactggatgc gcaggcgatc gagaaattcc gcgccgagtt gactcgctcg
    21481 acagcgctgg tgagaacggc gtgacaggaa gaatgccggc tgggcagtga gtcgcaacgc
    21541 ctacgcgatc gcgtcgacca cttccgtcag ctcgagcgcg agacgaccct ctggcagcag
    21601 acgcactgag gtgccaaacg actcgcactc tcgactcagc gttgcgctga gccagcgcgc
    21661 ttcgccctca tccgcgagct tgacctgcag acggcggatg cgggtcggcg tcatcgacaa
    21721 tcggaccagg ttccccgtgt cgcaatcgat ctcggggaag tacatcaatc caagctcgga
    21781 acggaagcgc tcgcgcccgc cgattccctc gtaatcgttc aacagatcgc cgcacccgta
    21841 gaggatgagc ttgccccggt agacctcgat cggcttcgga taatgagatg agtgaccata
    21901 gacgacgtcg acgccggcga cgtcgatcag ctgatgcgcg aagcgacgat gccgctcgct
    21961 cacctcatag ccccagttgc cgccccagtg gagcgacatg acaacgacgc cgccgccacc
    22021 tttctcccgt ctgatatggg cggccagctc ctcgacggta tgcaaagcca gatcgtcgag
    22081 cacgcacaga ccggaccggt attcggtggc gttccattcg ggagagatgc cgctatcggg
    22141 cgcgccgcac gcgaagacga gaacgcgctg ccgatccggg agatcgaagc atgctggcgt
    22201 gcgcgcctgc gcgaggttcg cacccgcccc cgcgacgctg agaccagcct cgcgcaggac
    22261 tcgcagcgtc tctgtcagcc cctcgcgtcc ccagtccagc gtatgattgt tcgccagcac
    22321 gcagcagtcg agatccgccg ccgtcagaca cccgacgttc cggggattca tccgatagtt
    22381 gatccccttt cccggccagg catcgctgct ggacgtgaca gcagtctcca gattcacgac
    22441 tcgcagagcc ggtcggcggc gctcgagctc gagcagcgtg tcaccccaga tgtacgcgaa
    22501 gtcggccggc cgggcgatcc gcccgtgtgc gcgctccgcc agctcgacgt actcgcatgc
    22561 ggacctgacg tgaggctcat acagctgtgg ggcggacgga tgcggcagga tctggtcgat
    22621 gccgcgcccc gtcatgacgt ccccgcacag aaagagcgtc atggtattgg cggcggctga
    22681 cggcgtgctc aactggatcc ccacgtcgag agacggcatt cgttcgcgac cagggccgac
    22741 tgcccggatt ccccggacag cacgccttgc agcgcgcggg tcgaccggaa caccgtgcaa
    22801 cccttgagcc ccagacgatg cgccgctgca aagatctcgc ggtggcgctc gaatggatag
    22861 tcgcgcggca gattgatggt cttggagatc gcgccgtcga caaacggttg cagcgccgcc
    22921 tgcatggcca ggtgcgcttc cgggtccaga tcttccgcgg tcacgaatgc atcgcctggc
    22981 ggcgccgcgc cgtgcagctc gcgccagcgg cgccaggcgt agtcgctcgt cgtgtgacgt
    23041 tcccaaccat cgaccgtacg cgtgcgtctg actccctcga aggcgaacac gggttcgacg
    23101 ccgctcgaaa cgttgccggc gagcagactg atggaaccgg caggcgcgag cgcaacgagg
    23161 tggctattgc gaatcccgta ctgcgcgatc gcgtcacgca gtctcgccgg caggcgctcg
    23221 acgaattggc tcgccagaaa gccgtccctg tcgaatgccg ggaacgcgcc cttggcaacg
    23281 gcaagctcga tggaagactc gtacgccgcg tcgcggatgg atcggagaat cgacgccgcc
    23341 tgctcgcgcg cggcggcgct gtcatagcgc agccccagca tgatcagtgc atcagcaagc
    23401 ccggtgacgc ccagtccgac tcgacgcgtg cgcagcgcct gctcgcgctg ctgcgggagc
    23461 ggatagcggg acacttcgag aatgtcgtcc aggaatcgca ctgcagtcgc cgttgcggcg
    23521 gtcagcgctg ccacatcgat ggaggcgctc gccgcaaacg ggtcgcgcac gaaccgcacg
    23581 aggttgaggg accccaggtc gcatgccccg tacggcggca acggcacctc gccgcacgga
    23641 ttggttgccg acagccactc acagtagtgg agattgttct cccggttgat ggtatcgacg
    23701 aacagcacgc ccggatcgcc acaatcaaag ctcgcttcga ggagcgcatt ccacaggctg
    23761 cgtgggctca ccgtgcgcag cacggcacat tcggtgggct caggcccgcc ccacgagcga
    23821 gtcaccgtcg gcagactccg ctcgccgggc agaagagcgc tccgcggaaa cacgagcggc
    23881 cacggctcat cggccgccag cgcctgcatg aactcatcag tgacctgcaa cgacaggttg
    23941 aagtgactca gcacgcgcga gcggtgcttc gccccgatga actggagcac gtcggggtgg
    24001 tcacagcgca gcgtggccat catggcgccg ccacgtggtc cgctgccggt gatcgtggca
    24061 caggcggcat cccacacctc catgaaggac acgggccccg tcgcgatgtt tccggtggcg
    24121 cgggcgcgct caccgcgcgg gcgcagcgtc gagaagtcgt agccgacgcc gccgccctgc
    24181 tgcatggtca gcgcaccttc cctgagggcc gagaagatcc cgtccatcga gtcttcgatg
    24241 gcgcccatca caaagcagtt gaggagcgtc gcccgcaact cggtgccggc gccggccaga
    24301 atgcggccgc ccggcaggaa acgaaagcca cgcagcagct ctgcgaactt cgcttcccaa
    24361 aggtcggcag agacgccctc gaccgttgcg gcagcgcgcg cgacacgtcg ccaggtgtgc
    24421 gcaatatcag tgtcgacggc gacatcgccg catcgatact gataccgctg cgcccagacg
    24481 cgccgagaga gctcaatctc gagtgggtca ggcatccgag ggccccatcg caatgcgcac
    24541 gcatctccct ggtggccagg cccccattat gccggcaacc gcgcatgacc acccatgccc
    24601 gccgcgcctc caccagtgca tgtctgcgtc acttgacccg catcgggacg gacaccacga
    24661 gtcgccacct ggcgcaccag ttacagtcgc tggacgaata catccgccgg caggtgctcg
    24721 cggatcgcga gctcatcctc atgcaccaga ttcttgtcga tctccagggc gatcgcctgc
    24781 ctcaacgact tcggcatgac ctcgcgcaga tcgccgagga actccggcgc cgccatgagc
    24841 acgagcctgt cgaagtgatt ctggcgttgt gcccgctcca gctcatccac gatccgccgc
    24901 gcaaacaccc cggcttcgtg cttgctgggc gtgcgctcac cgcctgtgct gtgatgggcc
    24961 acagcaccgc gtcgcttgcc gcccggcgca tgatcgaaca cgcgcccggg ccgatctgat
    25021 ttgtagtcgc gatcccgcag acgggcttca gggtcagtca tctgaccggc aagccgaagt
    25081 ttggattcgg gccgctcagc atcgtagaag aggacctgag cacgatcagc aacggcaact
    25141 cgaatacgca tgagactctc ccgccgacaa ggccaatcgg tttggtggac gctagccctt
    25201 gacgcagatc agcggctcgg tccgcgcgac cttgcgcgca agacccgccg catccgccgc
    25261 atcgacgacc gcagaaacat ccttgtaggc atccggcgct tcctccgcga caccgcgcat
    25321 cgacgggctg cgaatcagta tgccccgtgc ggcgagctcc tcgacgaccc gccgacccct
    25381 ccaggtacgc gtagcctgat gtcggctcat ggcgcggcca gcgccgtgac acgccgagct
    25441 gaatgccagc gattccgact cgctcgtgcc ggccaggatg taggagccag cgcccatcga
    25501 gccgccgatc aacacgggtt gaccgaaggc gcgcaagctc gccggaatgt cgggatgacc
    25561 tggaccaaag gcgcgcgtcg cgcccttgcg gtgcacatac agttttcgcg atgctccatc
    25621 gacccgatgc tcctcgacct tgcacgtgtt gtgcgagacg tcgtacagca acggcaaccg
    25681 tgcctgcggc aggatatcgg cgaagacctg gcgcacgaga tgcgtgatta tctgccggtt
    25741 ggccaacgca cagttgatcg ccgcacgcat cgcacccagg tatttctggc cgacttcgga
    25801 attgatcggc gcacaggcca gctcgcggtc cggcaggtga atgccgtgat cgctggccga
    25861 gacggccatc tgcttcagga actccgtgcc gatctgatga cccaggccac gcgacccgca
    25921 gtggatcatc accacgatgt cgccgacttc cagtccgaat gcctgcgcga tctgcgcatc
    25981 gtagaccgac gtgacttcct gcacctccag atagtgattg ccgctgccga gcgtgcccat
    26041 ctcgtcgcgc tgccggttct tcgcatgctg cgagacgttc tccggcttcg catgcagcat
    26101 ctgcccgtgc tcctcgatgc gctcgagatc ctccgcgacg ccccagcccg cgtgcaccgc
    26161 ccagtgcgcc ccgccggtga gcatcgcctc catctcctgc acattgagat ggatcaggcc
    26221 cgtgctgccg acgccggccg gaatccggta gaagaacatg tccgccagct ctttctgcac
    26281 ggcgagtatg tccgggcgcg tgaggcccgt atgcaggcaa cgcacaccgc acgagatgtc
    26341 gaaccccact ccacctgccg aaatgacgcc gccggcatcg ggatcgaatg cagcgactcc
    26401 gccaatggga aagccgtaac cccaatgcgc gtccggcatt gcgtatgacg cttcgacgat
    26461 accggggagc gtggcgacgt tgactgcctg ctcgtatacc ttctggtcca tggcgcggat
    26521 gagcggctcg ctcgcgtaga tcacggccgg cacgcgcatg gggcctcgcg gggcgatctc
    26581 ccacacgtgc tcggagcggc ggttgaacag cgcgacgtcc atgatgaccg ggattgaagc
    26641 cttgcctggt caccgggaca accccgctat atcccttatg cagcaagggt aagcagcgat
    26701 ttcgcgattt ccatgcgcgg ccggcagcct gctcgcaggc ccccgcatcg ccccctccgc
    26761 gacgaggcgt gcttcacgaa ttgcctgcgc caaccgcccc gctcagacat ccaccaccgt
    26821 ctgcgcgacc cactgcgacg cttcgctcgc aacgcgcagg gccgtatagg ttgcgccttt
    26881 cacttcgact gccggctggt gccggggcgc atcgatgcgc tcgccccagg cctcggcgct
    26941 gagccgcgtg ccgtcgatgg tgacgatgaa cttaccgaag accatgtgtc gagtggccat
    27001 ctcatagacc aggctgttca gccactccgc gaacagcagt tcctcatcgg gtgcgctgca
    27061 ttcgatcgtc acgcgctccc gggcctcaac cgcatcgggc tcggtaacga gggctgtcat
    27121 ggcaagggcc ccttgctcga acgcttctgg aagagtggac ccgagcccac gaacacccat
    27181 gtctgcgtcg tgggcgaagt gctcccaacg tggcaggcgc ggatgacccg cggcctccat
    27241 cagcgcgcgc gatctctggt cgtctcctgc tcctctgcgt agacctcggg gggccgcccc
    27301 tcctcttctt cctccagttc atccggcgac ctcgcatcct gccagggctc ttcagctgct
    27361 tccacggact cgagcggctg ccgccagtct tgcggcgtcg tacccgtctc cgagagcccg
    27421 agatcgtccg gctcgatatc gtcgaacggc ccggtccagt cctccggcgg ctccgccgtt
    27481 tcgatgtcca tcgcctgcca cgcaatggtg tccagttcct ccacgtcccc gtcgaaggac
    27541 tggatttcga tgtagtcttc cgccgcatcg gccgccacga cccggaacat cccgcccttg
    27601 tcccggtaca cgtaccactg tccgacaatg ggatccagtt cgttgggcat gaccgccgcc
    27661 cctcttctac aggcttgcta ttcgatcgaa gcatctgctt tcgtcccctc ctgccaatcc
    27721 ggagtggtac cgaagtgacg ccgggatgtc cctattccag ctgcgatgcg agcagcccgg
    27781 cggcggggta atcgccgcgc cacctgcgcc gcgatgcacg accgaaacgc tgcccgtgga
    27841 attccgggta gacccgagcg cgcaagcggc caacatgcct gtatcgcccg ttggagagac
    27901 ctcatgcaaa cgccgctgcg aatcaccttc cggcacatgg aaccatcgcc cgccgtcgag
    27961 gctcgtgtgc gagagcacgt cgagtggctg gaacgcttct atgatcggat caccggctgc
    28021 catgttgtcg tcgaacgacc gccggcacat cggcacaagg gcgcgccgtt tgacgtgaag
    28081 atcgacctga ccgtgcccgg tgatgaaatc gtcgtacgca gcgaacgcgc cgagcacgcc
    28141 gctcacgtgg acgtctacgt cgcgctgcgc gatgtcttcg acagtgcccg gcgacttctg
    28201 caggaccacg cacgcaggca acgcggtgac gtcaagcagc acagcctgcc gacgctcggc
    28261 accatcgacc agatcgagca gggcttcgga cgcatcgccg ccgatgacgg ccactacgtc
    28321 tactttcaca gcaatagcgt tcacggcatc ccgttcgacg aactgaccgt cggcacggtc
    28381 gtcgagttcg aggaggaaca gggtgacgac ggaatgcagg ctgccgcagt gaggctttgg
    28441 ggtggcgcac acgaatcacg caaaccggcg tagctgaaat cacgccgcgc gggggcacgc
    28501 atgaaccagc accacggcac gcagacgacg atcgcgctcg gcaccggcgt cgaactgaag
    28561 ggcttcctcg aaggttcgga agacgccagg ggattgatcg tcttcgtgca cggcagcggc
    28621 agcagccgat tcagcgcccg caacaatcag gtcgcgcagt tcctgagagg ccgggggttc
    28681 gcgacactgc tcatggacct gttgaccaca agcgaagaac gggtagacgc catcacgcgt
    28741 gagctgcggt tcgacatccc gttgctcgcc gagcgcgtca cgggaacgct cgactggttg
    28801 gccaggcacg atcgggtcgg aagattgccg attggtctct tcggcgcgag cacgggcgca
    28861 gcggccgcac tcattgccgc ggcgtcgcag ccgacgcgtg tcagcgctgt cgtgtcccgc
    28921 ggcggcaggc cggacctggc cgcagacggc ctcgaggcgg ttcaaacccc gacgattctg
    28981 atcgtgggga gcgacgatat cgacgtgctg cggttgaacc agatggcaat ggcacgcatt
    29041 ccggcggaga cgaagctcgt caccatcgaa ggcgccaccc acctgttcga ggagcccggc
    29101 accctggatc aagttgcaca gcacgcggcg gactggttcg agcgacatct ccgcgaccgg
    29161 agcgccggcg gccagggatt tccgcagtcc gcatcgtagt ttaccgtgtc gatacggacc
    29221 cgccccggtg caacacttcg gtggcgggca gcctgctcac caggcaacgg aggggttctc
    29281 agcttgaacg agcggccgac gtccttcggt acggtgggaa gcatctgcag ccgcgaggtc
    29341 atcgtcgcga cgcccggcga gcccgtgcga accgcgttgc agctgatgca ggctcggcga
    29401 gtcggtagtg tcgtgatctg cgatgacctg agcgggcgac gaatacccgt cggcatcgtg
    29461 acggatcgcg acatcgcgct gagtctgctt cagcgggccc cggatgtgac cgtcattccg
    29521 ctgcgcctcg tgatgtcctc ggaccctctc gtgctgttcg aggatcagga cgtgagcgag
    29581 gcactcgccg ccttgcgggc gcggggcgtg cgccgtgcgc ccgtcgtcga tgctgcgcga
    29641 tcgctggtcg gcatcgtgac gatcgacgat ctgctcggcc tgatcgcccg gcagctgggg
    29701 gatatggccc ggctcatcga gcggcagacg ctatgaacgc agagcccgcc acgagtgcgc
    29761 tgacgcgcgg cccggcccat gcgcagcgcc tgctccgagc cagcagccag cactggaagg
    29821 cggctggcgc cgccccggct ggagacaaag agacgcactt caacgatgta agtggcggag
    29881 cggacgggac tcgaacccgc gacccccggc gtgacaggcc ggtattctaa ccaactgaac
    29941 taccgctcca catggtgggt gctgagggga tcgaaccccc gaccctcgcc ttgtaagggc
    30001 gacgctctac cgctgagcta agcacccttc aagccttcgg aaggcaagct ccagaaggcc
    30061 gcggattcta gaggatcggg tcgccggcgt aaactcgcgt ttacgtgccg ccgccacagc
    30121 gtgccgcccg tgcgacttaa tgcgcctgca cgccgccctt gcccgcccgt cgcgccgcac
    30181 gccgccgctt gggatcggca gcggccggcg catccgccgc cgaggcgatc ggcaccggac
    30241 gcgccgccag cgcgatctcg agcacttcgt cgatccattt cacgggcttg atcctgagat
    30301 tgcccttgat gttgtcggga atctcgacga ggtccttctc gttgtcaatc gggatcagca
    30361 ccgtttctat gccgccacgg tgtgcggcca ggagcttctc cttcagcccg cctatcggca
    30421 gcacttcgcc gcgcagcgtg atctcgccgg tcatcgcgac gtcggaacgt accgggatct
    30481 tcgtgagcgc cgagaccagc gccgtgcaca tgccgatgcc ggcgctcggg ccatccttcg
    30541 gcgtggctcc ttcgggcatg tgcaggtgca cgtcgtgctt ctggtagaag tcgggctcga
    30601 tgccgagcac gttggcgcgg ctgcgcacca ccgtcatggc ggcctggatc gattcctgca
    30661 tcacctcgcc gagctggccg gtgtgctgca gcttgccctt gccgggcact accgcggcct
    30721 cgatggtcag cagttcgcca cccacctcgg tccacgcgag gcccgtgacc tggccgatac
    30781 ggctctggtc ctcggccttg ccgtagcgga agcgccgcac accgaggaac ttgtcgaggt
    30841 tcttcggcgt taccgtgaca gccttgtcct tcttcgcgag cagctgctgc ttgacggtct
    30901 tgcggcagat cttggcaatc tcgcgctcga ggttgcgcac gccggcctcg cgcgtgtagt
    30961 agcgcacgat gtcgagcagc gcctcgtcgg tgaccttcag ctcgtccttc ttcacgccgt
    31021 tgttcttcat ctgcttcggc acgagatagc gccgcgcgat gttcagcttc tcgtcttcgg
    31081 tgtagcccgg caggcggatc acttccatgc gatcgaggag cggcgccggg atgttcaggc
    31141 tgttggccgt gcagacgaac atcacctgcg agaggtcgaa atcgacctcc aggtaatggt
    31201 cgttgaacgc cgtgttctgt tccggatcga gcacctccag cagggccgag gacgggtctc
    31261 cacggaaatc catcgacatc ttgtcgactt cgtcgagcag gaagagcggg ttgtgcacgc
    31321 cggtcttcgc catgttctgc acgatcttgc cgggcatcga gccgatgtag gtgcgccggt
    31381 ggccgcggat ctcggcctcg tcacgcacgc caccgagcga catgcgcacg aacttgcggt
    31441 tggtggcacg cgcgatcgac tggccgagcg aggtcttgcc gacgcccggc ggccccacga
    31501 ggcacaggat cgggcccttc agcttgtcga cgcgttgctg caccgcgagg tattcgacga
    31561 tgcgctcctt gaccttctcg aggccatagt ggtcctcgtc cagcaccttc tcggctcgcg
    31621 cgatgtcctt gatgaccttg gtgcgcttct tccacggcac cttcaccagc cagtcgacgt
    31681 agttgcgcac cacggtggct tccgccgaca tcggtgacat catcttcagc ttgccgagtt
    31741 cggccatcgc cttgtcgcgc gcctccttcg gcatgccggc cttctggatc ttctgctcga
    31801 tctcggcgag ttcgttggcg ccatcctcca tgtcgccgag ttccttctgg atggccttca
    31861 tctgctcatt caggtagtac tcgcgctggc tcttttccat ctgcgacttg acgcggccgc
    31921 ggatgcgctt ctcgatctgc agcatgtcca tctcgccttc gatcgcgacg aggatgtgct
    31981 cgaggcgctg cttgatgtcg aggatctcga gcaccttctg cttttcgggc agcttgagcg
    32041 ccatgtgcgc cgccacagta tcggcaagac ggccgggctg ctcgataccg gcgagcgcgg
    32101 tcagcacctc gggcggcact ttcttgttga gcttcacgta ctgttcgaac tgcgagatga
    32161 ccgagcggcc gaggacgtcc atttccttct cgtcatagac gtcgctgtcg ggctgcaacg
    32221 tgacatcgca ggcatagaac tcgccggggt ggatgcgatc gatctgtgcg cgatcgacgc
    32281 cctcgaccag caccttgacg gtgccgtccg gcagtttcag cagctgcagg atcgtggcga
    32341 ccgtgccgat tcggtacagg tcgtcgactt tgggatcgtc gacgtcggcc tgtttctgcg
    32401 cgaccagcat gatgcgcttg tcggccttca tcgcgacatc gagcgcctgg atcgatttct
    32461 cgcgacccac gaacagcgga atcaccatgt gcgggtagac gaccacgtcc cgcagcggca
    32521 gaaccggcag cttctcgaga ctggtggtct tcggggtggt cgggggggat tcgggcacag
    32581 gcaggtcctc gcgcaagacg gttatagtct accctcggag ataggggcgc accgcgccga
    32641 tttcaagcgc ggtgccgccg gaaggaccag caggtcagct tgcgatgccg tgcgcgcggc
    32701 gcggcggctc ttcggcggat tcggacttgt agacgatata cggcttcgtc tggccgtcga
    32761 tcaccgtctc gtcgatcacg accttggaca ccccgcgcag cgacggcagc tcgtacatgg
    32821 tgtcgagcag gatggtctcg aggatcgtgc gcaggccgcg cgcgccggtc ttgcgctgca
    32881 tggcgcgctg cgccacggcc ttcagcgcct cctcgcggaa ctcgagctcg gcgccctcca
    32941 tctcgaagag gcgcttgtac tgcttggtga gcgcattctt cggctcggtg aggatcgaaa
    33001 ccagcgccgc ctcgtcgagt tcctcgaggg tggcgaccac cggcaggcgc ccgacgaact
    33061 cggggatcag cccgtacttg atcaggtcct cgggctcgac ctcgtggaac acgtcggggc
    33121 gatccaccgc ggtcatgtcg cgcacgtcgg aggtgaagcc gatgccgccc ttctgggtgc
    33181 ggttcaggat gatcttctcg agcccggaga acgcaccgcc gcagatgaac aggatgttcg
    33241 aagtgtcgac ctgcaggaat tcctgctgcg ggtgcttgcg cccgccctgc gggggcaccg
    33301 acgcgaccgt gccttcgatc agcttcagca gtgcctgctg gacgccctcg cccgacacgt
    33361 cacgcgtgat cgacgggttg tcggccttgc gcgagatctt gtcgatctcg tcgatgtaga
    33421 cgatgccggt ctgcgccttc
//
LOCUS       DAOQVY010000010        33373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_1524_length_33373_cov_5.765562, whole genome shotgun sequence.
ACCESSION   DAOQVY010000010 DAOQVY010000000
VERSION     DAOQVY010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 33373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33373
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1524_length_33373_cov_5.765562"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..774)
                     /gene="petA"
                     /locus_tag="PKN91_01960"
     CDS             complement(160..774)
                     /gene="petA"
                     /locus_tag="PKN91_01960"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNS26863.1"
                     /translation="MTDIAATADQEIDHSRRRFLTAATVGTGAVGVGFALVPFIKSWK
                     PSERARALGAPAEVDVTKIEPGQMVIVSWRKQAIYVVHRTPEMVAAIGPDESMLKDPA
                     SDASIQPEYAKNTLRSAKPEFLVIVGICTHLGCLPKQHFERGEAALGADWPGGFFCPC
                     HGSKFDLAGRVMSGSPASTNLVVPPHAFRGDTLVIGVDPQEGVA"
     gene            868..2004
                     /locus_tag="PKN91_01965"
     CDS             868..2004
                     /locus_tag="PKN91_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNS26864.1"
                     /translation="MTPQRLGRALVFLAGSVIGGLALAFVIVFLRPDLLRAPPPPAPA
                     APAPAITAPPAPVVSYADAVARAAPAVVNIYSERVVKERLPPATLGELFGGVMPRYRQ
                     RIEQTLGSGVVVDAAGHIVTNHHVIAQAERVSVQLADGRQGEASVVGRDPDTDLAVLR
                     VDLGEVPVMPLGRSDQLRVGDVVLAIGNPIGLTQSVTQGIVSGTGRGQLGVATFENFI
                     QTDAPINVGNSGGALVNTHGELVGINTAVLAKNLGVEGIGFAIPVNLVRGVVQEILSH
                     GRVIRGSIGVVLGEVDAAQAERLGLSRAGVVITNLFQGSPALEAGLRPGDIVESIAGN
                     AVRGQQDALSRIATARPGETVDVVIVRGTETITVPVKVTERRTS"
     assembly_gap    2185..2284
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2571..3830)
                     /gene="murA"
                     /locus_tag="PKN91_01970"
     CDS             complement(2571..3830)
                     /gene="murA"
                     /locus_tag="PKN91_01970"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNS26865.1"
                     /translation="MDKLQIQGGVPLEGEIRISGAKNAALPILAGTLLADGPVTVANV
                     PHLQDVTTMIELLGRMGVDVTIGERMHVEVDPTTLRECYAPYELVKTMRASILVLGPL
                     LARHGRADVSLPGGCAIGARPVNIHVAGLQAMGADISIEGGYIRARASRLKGARLVLE
                     AVTVTGTENLMMAAALAEGETVIENAAREPEVVDLANFVNALGGKVRGAGTDTIVVEG
                     VERLGGAHYVVLPDRIESGTYLVAGAITSGHVRIKNTRPDHLDAVLGKLVEAGARIDV
                     GENWIEIDMRGKRPKSVDIRTAPYPGFPTDMQAQFAALDTIADGVGTIIETIFENRFM
                     HMLEMRRLGAEIRLEGNTAIIRGVPKLTAAPVMATDLRASASLVLAGLVAEGRTDIER
                     IYHIDRGYETIEEKLSQLGAQIRRVPG"
     gene            complement(3831..4076)
                     /locus_tag="PKN91_01975"
     CDS             complement(3831..4076)
                     /locus_tag="PKN91_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HNS26866.1"
                     /translation="MQPSEVAELIRAALPGAQVHVQSEDNVHFAALVIAPGFAGKRGI
                     ARHQMVYAALGERMGGEIHALSIDALTPEEAAARAAG"
     gene            4122..5129
                     /locus_tag="PKN91_01980"
     CDS             4122..5129
                     /locus_tag="PKN91_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNS26867.1"
                     /translation="MNAATDAPGSPATTDAGLLAAGRRALRIEATAVESLLERLDARF
                     AAACRLCLACAGRIVVTGMGKSGHIGGKIAATLASTGSPSFFLHPAEASHGDLGMITR
                     GDVVVAISNSGETPEVVTLLPHLSRLGVPLIAMTGATDSTLARAAAVVLDISVPEEAC
                     PLNLAPTASTTVTLAMGDALAVAILDARGFTEQDFARSHPGGALGRRLLLHVEDVMRR
                     GDDIPRVAPDALLKEGLVEMSRKGLGLTVIVDESGRSLGVFTDGDLRRILDQPVDVQR
                     TRMAEVMTSPGKAIGPRELAAEAVHMMELHRITALPVIDTAGRLIGALNVHDLLRAGV
                     M"
     gene            5132..5656
                     /locus_tag="PKN91_01985"
     CDS             5132..5656
                     /locus_tag="PKN91_01985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HNS26868.1"
                     /translation="MVRATTLPAAIRLLVLDVDGVLTDGRLYYGPRGEVLKVFHVLDG
                     HGIREAQRAGIEVAVISGRDSAAVRRRCRDLGIRHVFQGVADKLPVFERLCRRLRILP
                     IEAAIIGDDVPDVPMMQVVGLAFAVPDAHQAARRAADHTTSARGGRGAVREVCDLLCA
                     ASARRPRKSARKRR"
     gene            5653..6213
                     /gene="lptC"
                     /locus_tag="PKN91_01990"
     CDS             5653..6213
                     /gene="lptC"
                     /locus_tag="PKN91_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNS26869.1"
                     /translation="MTRWLIGFAVIVTVIASIVLSRSARAPDGIGASLPTAADPGYLA
                     RDAEVIETGDDGRPRYRLDAELIEQAPNDPTITLSRPVMHYADPRGGTWRASANQGTV
                     APPSDVVELTGDVLLTGTLADGGDPAHLTTNRLTFDAGRDVAHTSAPVTIEWSGHRLT
                     ARGVRADLGAETLRLESAVHGNFLPP"
     gene            6191..6841
                     /gene="lptA"
                     /locus_tag="PKN91_01995"
     CDS             6191..6841
                     /gene="lptA"
                     /locus_tag="PKN91_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HNS26870.1"
                     /translation="MAISCPPDRRRPARLLAGLALAALCAARTMPAAEPDPRTLPINV
                     DAASSDVDYRSNTVVFRDVVITQGGARVAAANARATGLDFVDSTWEFTGRVRIDRDGG
                     TLESDRAKVRFRDNVLESATIEGKPARFEQQLVRTPGKAHGRAETIVYDMAANRVTFT
                     GDAWLTDGRNEISGQELVYNVADQRVLAETQPGDTDRVRITIRPQKDATPADEPAP"
     gene            6838..7563
                     /gene="lptB"
                     /locus_tag="PKN91_02000"
     CDS             6838..7563
                     /gene="lptB"
                     /locus_tag="PKN91_02000"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNS26871.1"
                     /translation="MSRLRVEGLAKRYGKRSVITDLSLEVSSGEVLGLLGPNGAGKTT
                     AFYMVVGLVPADAGHIWFDDTDMTHMPMHRRARLGVGYLPQEASVFRKLSVQDNVLAI
                     LETRADLDRAGREARLEEILEELRIAHVRDSLGMSLSGGERRRVEIARALAAEPRFML
                     LDEPFAGVDPLSVLDIQRIIRELTAKGIGVLITDHNVRETLGVCDRACIVSSGTVIAA
                     GNAEEILANAQVREVYLGETFRL"
     gene            7626..9056
                     /locus_tag="PKN91_02005"
     CDS             7626..9056
                     /locus_tag="PKN91_02005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNS26872.1"
                     /translation="MLKPSLQLRLGQSLTMTPQLQQAIRLLQLPALELQAHIRELLES
                     NVMLEAVEEPEQTGIFEAIQSPAERVAPQREEPRVDVEVITDNWSGESAGGSDSPWSP
                     DDDDRQQDYAAQSDTSLQDHLLAQLEVANLDARDLAIARAIVDAVNDDGYLFEPVAEI
                     AATLRPDVECTDEEVLAVLAAVQTLDPAGVGARSVAECITLQLRQLDADTPGLATALE
                     IAARHLDAVAKNELALLRRELRVTEDELAEALALVRTCQPRPGSAVGSGSPEFVVPDV
                     FVRRTERGWSVEINPSTLPRVRLNQGYASALGRNSSHATLRAQLQEARWLLKSLEIRN
                     DTLLKVARAIVERQADFLEHGEEHMRPMILKDIAAAIEMHESTISRITSGKYMHTPRG
                     VFELRYFFSSHVGGGEAAETSSTAIRAKIRKLIRDEDPAAPLSDSRIAELLSAEGIPV
                     ARRTVAKYREAMRIAPSNERRRAASK"
     gene            9074..9394
                     /gene="raiA"
                     /locus_tag="PKN91_02010"
     CDS             9074..9394
                     /gene="raiA"
                     /locus_tag="PKN91_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNS26873.1"
                     /translation="MQVTVTGHHVEVTPPLKSYIEKKLERIVRHYDSVIDVHCVLTVE
                     KQRQKAEATLQLRGGSIHADAIDEDMYAAIDALADKLDRLVRKHKEKLQDHHAAEARK
                     ATRQ"
     gene            9418..10293
                     /gene="rapZ"
                     /locus_tag="PKN91_02015"
     CDS             9418..10293
                     /gene="rapZ"
                     /locus_tag="PKN91_02015"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNS26874.1"
                     /translation="MRLVVVSGLSGSGKSVALHMLEDLGYYCIDNIPAALLTPLISHT
                     VRSAEAAYERIGVGLDARNTAAEIATVPKLIAELKKSGIQCEVLFLTTSDDELLRRFA
                     ETRRKHPMSRNDIGLREAIALERDLLEPMMYAADQVIDTSRMGVHALRDRVLKSADER
                     ATPRLAITFESFGFKRGIPGDADFVFDARSLPNPYWEPQLRPLTGQDPEVIRFLEAEP
                     AVARLIEDIANFVEARIPEHTAANRRYLTVAIGCTGGQHRSVYIANQLAGRFSAHHPH
                     VSTRHNELRERPGAH"
     gene            10312..11379
                     /locus_tag="PKN91_02020"
     CDS             10312..11379
                     /locus_tag="PKN91_02020"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNS26875.1"
                     /translation="MSALRNLAGLALGALLTSACNQDANPAMASVSGTATYRERMALP
                     PGAVFEVRLEDVSRAGAAAGILAETRVESPGNPPFAFTLSYDPAHIDATHSYSIRARV
                     LHGDAVLFTSDTHTPLPADDSSAPLEILMVRAGAARAGSPDVPDATPGPGLLRGTYSY
                     MADAGLFTACGTDERIPVAMEGDNAALESGYRKVVPEPGATLVATVIGRIEAREPMEG
                     PVRPHLIVEQFVNVVAGTCETPPVVALEDTYWQLAQLGGTPVALAAGQRAPYFVLDSA
                     RHRVSGYAGCNRMMGGYSVDGAKLAFTQMAGTMMACLQGMEIEQALHEALKRVTAWRI
                     EGNRLDLLDADGAVAAAFTAV"
     gene            11464..12822
                     /gene="mgtE"
                     /locus_tag="PKN91_02025"
     CDS             11464..12822
                     /gene="mgtE"
                     /locus_tag="PKN91_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNS26876.1"
                     /translation="MSDTDTTPLKSGRLSALRDALDQGTLRAAHRMVNAMHPADIALL
                     LESLPPAQREIVWEMVDPELEGDVLVELNENVRAGFIEEMGAEELVAATEGLEVDDLA
                     DLLDELPETVTQQLLHSMDQRDRERLRTVLGYPEDSAGGLMNTDTVSVRADVPIEVVL
                     RYLRMRGELPEGTDSLFVADRHDRYLGSVPLTRLLTADPDHLVGDCLDTEAPRIDPDM
                     HAHDVSRLFEDRDLVSAAVVGSEGKLLGRITVDDILDVIREEAEHSVMSMAGLDEEED
                     VFAPVLPSARRRLVWLAINIMMAFIGAFVVASFEDTIEKVAVLAALLPVVSSMGGIAG
                     TQTVTLLIRGLALGKVDWTNARWLLFKEIAVGGLNGIVVASTVALVTLALIGRWQIAA
                     IVAAAMLMNLLAAATVGVLIPLALRRLRIDPALSAGVILTTFTDCIGFATLLGLGALF
                     LV"
     gene            complement(12827..13969)
                     /locus_tag="PKN91_02030"
     CDS             complement(12827..13969)
                     /locus_tag="PKN91_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNS26877.1"
                     /translation="MRQDTIISGRPVQALILTGGGARSAYQVGVLRALSEMLPRRGIP
                     FPVLVGTSAGAVATAALAAEARRWRGAVASLEHVWASFHSDHVFRVGSVHMLASGLRW
                     LVALVSGGMLANPPRSIFDNAPLAELLGRNIDFGAIRHSVSTGMLRAVALCSTGYTSA
                     HSVAFFDGAEEIQEWARAHREGRRVDLTLSHLMGSLAVPFLFPPVELGTEYFGDGAMR
                     QLAPMSPAVHLGAERVLVIGVRAANAAGLSQVSQLPRSPTPGQLFGFMLDTLFTDGIY
                     ADLEQLIRLNAMARTAPEAVPDVRLIEAMAISPSIDPREIAARHLHELPRSLRAFLRV
                     IGARGVAGHQLASYLMFESGYTSELIELGYGDAVQQRDRLRAFLLG"
     gene            14054..14602
                     /locus_tag="PKN91_02035"
     CDS             14054..14602
                     /locus_tag="PKN91_02035"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNS26878.1"
                     /translation="MLPDRSTLLAAILAMAAVAMPALAQDSPAVGSAAPTFKLQDQNG
                     AWRTLEEQRGKWVVLYFYPKDNTPGCTTQACEFRDNIFAFREAGAVIYGISVDDVKSH
                     EEFAREHSLPFTILADSAKETAKSYGVLYKAMGLLELARRDTFIIDPQGRIAKHYVKV
                     DPKGHSELVLADIKALQAAAKG"
     gene            complement(14571..15821)
                     /locus_tag="PKN91_02040"
     CDS             complement(14571..15821)
                     /locus_tag="PKN91_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNS26879.1"
                     /translation="MLVRFFGELRAAGVPATLPEFLALLEALQARVAWLSVEDFYFLA
                     RVCLVKDERHYDRFDRVFAEHFKGIEKAFEAITASVPDEWLRSLAERVLTEQEKAQVE
                     ALGGWQKLMETLRQRLAEQRERHEGGNKWIGTGGTSPFGAGGYNPEGVRIGQGGSRHR
                     RAVKVWDRREFANLDDSLELGTRNFKMALRRLRRFAREGAAEELDLDGTISATARNAG
                     HLDLKIVPERRNAVKVLLLLDVGGSMDEHVRVCDELFSAARSEFRHFEHYYFHNFPYE
                     SLWKDNRRRHTERIPTDQLIRTYGPDYRLIFVGDAAMSPYEITMPGGSVEHWNAEAGA
                     TWLRRLTAHFRHFVWLNPEPEKRSAYTPSTVITRELVEGRMYPLTLAGLDRATAELRR
                     RGSMPPQPPPAAAGISPSPPPAAP"
     gene            complement(15811..16662)
                     /locus_tag="PKN91_02045"
     CDS             complement(15811..16662)
                     /locus_tag="PKN91_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNS26880.1"
                     /translation="MTNSRFSGSERYIATPELAMAVNAAVTLSRPLLIKGEPGTGKTQ
                     LAEEVAASLGRPLYEWHIKSTSKAQQGLYEYDAVSRLRDSQLGDGRVHDIANYIVKGK
                     LWEAFEANTQPVLLIDEIDKADIEFPNDLLRELDRMEFYVYETRQLVKAKHRPIILIT
                     SNNEKELPDAFLRRCFFHYIRFPDEVTMRRIVDVHFPGLKQSLLAEALASFFRLREVS
                     GLKKKPSTSELLDWIKLLVAEDIPPEALRGDDAKKTLPPLYGALLKNEQDMHLVEQVM
                     FLSRRAR"
     gene            complement(16659..19061)
                     /locus_tag="PKN91_02050"
     CDS             complement(16659..19061)
                     /locus_tag="PKN91_02050"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNS26881.1"
                     /translation="MLRFLRRLAYVLLAAAIAAVIGAWLLMRASLPQLDGEIEVARLA
                     APVTIERDALGTPTVSGSDRAALAYGLGFTHGQDRYFQMDLARRLAAGELAELFGEVA
                     LEQDRKARLFRFRRVARALAESLTREERAVVDAYVDGVNAGLASLRSRPWEYWLLRAV
                     PRVWRAEDTALVVHSMWWQLQYHDFAGEQRRLALTEALTQRLGGARAAAVLGFVLPAG
                     SAWDAPVESQSPPLAEPAIPGPEVWDLRTGAATDAPSPREAAAARRTGASRGIGSNNW
                     ALAGARTASGAALVANDMHLGLDVPPVWYRARLTTHDGRYDLNGVTLAGVPALVAGSN
                     GRIAWGFTNSYGDWLDLRWTQCDLEAGTWRDLAGVEQPFDIDTARIRVRGGQTERLRI
                     RSGAEGVVFATREQDGQGECQLATWVATQPGATTLGLLGLESAASVADALEVAAGVGI
                     PHQNMMVGDRDGHIAWTLLGRIPATRGPLRAAGGAYLAPSMQPRVVDPATGSLWSANA
                     RVVGGEAGALIGDDEDAHGVGFDLGARAMQIRDGLAALRAPATPADMLAIQLDDRALY
                     LAPWRQLLLELLDAAALDGAPRRAAFRELISAPMDAAAVDAVGYTLVRRTQQQVVDAT
                     WSMVLAGAGLAGEAYPPPAQFAATAWQLATARPAHWLAPAYVDWRAFLLAQVDAAATE
                     LERQCGALARCAWGRQRAVGIAHPLAGALPLLGRRLLAMHVPELPGDHDMPRVQGAAF
                     GASERFAVSPGHEADAYLTVAGGASGHPLSPFFRAGFDDWARGAPTPFLPGPAQHKLV
                     LK"
     gene            complement(19062..20495)
                     /gene="pyk"
                     /locus_tag="PKN91_02055"
     CDS             complement(19062..20495)
                     /gene="pyk"
                     /locus_tag="PKN91_02055"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNS26882.1"
                     /translation="MQRRTKIVATLGPATDDCLVLTDMVRAGLDVARINFSHGDHADH
                     ARRVALVREAAARAGKIVGILADLGGPKIRIESFRDGRVRLAEGQAFALDPRIAADAG
                     VQHEVGIAYGNLHRDVSAGDTLLLADGQIVLDVTAVDGPRVETVVRIGGELSNRKGLN
                     RKGGGISAPALSDKDRDDIRFAAGQEIDYVAVSFARDAADIREARAKLREWGGQGRIV
                     AKIERHESVENLPSILDETDAVMVARGDLGVEMGYAEITGLQKTILHQARGKFRVAIT
                     ATQMMESMIQSPVPTRAEVSDVANAVMDGTDAVMLSAETAVGRFPVKAVEAMAQVIEG
                     AEKYQLSIARARDRNVGHFTRTDEAIAMAVMYTANHMRVRAIVALTESGSTPLWMSRI
                     RSDIPIYAFTRHPATSRRVTLYRGVYPVPFDVTEADGAHVQESIFARLLELRLVDSGD
                     RVIITTGALTGVSGGTNAMQILEVTRE"
     gene            complement(20540..21721)
                     /locus_tag="PKN91_02060"
     CDS             complement(20540..21721)
                     /locus_tag="PKN91_02060"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNS26883.1"
                     /translation="MKLLRMSDVDLAGKRVMIREDLNVPIDKGVVTSDARIRAALPTI
                     RRAMQQGAKVIILSHLGRPVEGHYSAENSLAPVAVRLSELLGKPVPLRENWLAGGVDI
                     GYGEAVLCENVRFNKGEKANDATLARRMAALCDVFVMDAFGTAHRAEASTHGVATFAP
                     VACAGPLLVAELEALERALDAPARPLVAIVAGAKVSTKLTVLESLIAKVDKLIVGGGI
                     ANTFLAATGVAVGKSLHEADMLDIARSLLEQARARGTEIPLPTDVVVAREFAATAHAD
                     VRSIHDVGPEELILDIGPDTAERFAALIEAAGTVIWNGPVGVFEFPQFGEGTKAIANA
                     IARSKAFSLAGGGDTLAAIEKYGVAEGISYISTGGGAFLEFVEGKTLPAVAALEARGS
                     G"
     gene            complement(21730..22743)
                     /gene="gap"
                     /locus_tag="PKN91_02065"
     CDS             complement(21730..22743)
                     /gene="gap"
                     /locus_tag="PKN91_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNS26884.1"
                     /translation="MAIKVGINGYGRIGRNTLRALYETKRTHEIQIVAVNDLGDANTN
                     AHLTRHDTAHGKFPGEVRVDGDSMLINGDRVRVFAERDPAKLPWGELGVDIVIESTGH
                     FTSKAKAGAHLKGGAKKVLISAPGGSDVDATVVYGVNHGDLKASHEVVSNASCTTNCL
                     APVAKVLHESIGIVGGLMTTIHSYTNDQVLTDVYHSDLRRARSATMSMIPTKTGAAAA
                     LGLVLPALKGKLDGYAVRVPTINVSLVDLTFTPQRATTVEEIDKLMLAASQGPLKGVL
                     AYTSEPLVSIDYNHDPHSSTYDTTMTKVIDGKLVKVCSWYDNEWGFSNRLLDVVSAWS
                     RAK"
     gene            complement(22780..24777)
                     /gene="tkt"
                     /locus_tag="PKN91_02070"
     CDS             complement(22780..24777)
                     /gene="tkt"
                     /locus_tag="PKN91_02070"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNS26885.1"
                     /translation="MPTRRDLANAIRALAMDAVQAAKSGHPGAPMGMADIAEVLWRDV
                     LKYNPRNPRWVDRDRFVLSNGHASMLLYAVLHLTGYPLTLEEIRNFRQLGARTAGHPE
                     RDLAIGIETTTGPLGQGFANAVGMALAEKMLAAEFNRPGHEIVGHHTYVFVGDGCLME
                     GISHEAASLAGTLKLGKLIAVFDDNGISIDGKVQGWCTDDVARRFAAYGWHVIKDVDG
                     HDSAAVASALRKARNHKNQPTMILARTIIGFGAPNKQGTKSVHGEALGVEEVSAARTT
                     LGWPHGPFEIPDEIRAQWDRRAQGEAAEKSWQRRFRRYRKAFPELAAEYERRMAGRLP
                     DNHAALLDAALAAARALESPQATRVSSGAVLDVIGPGLPELLGGSADLTGSVNTLRKD
                     SRTVTADNAGGNYIHYGVREFAMTAMMSGFTLHGGFRPYAGTFLVFSDYARNAVRLAA
                     MMHQPVILVYTHDSIGLGEDGPTHQPVEHLASLRAMPGLSLWRPADAVETAIAWRMAL
                     ERMNGPTALVLTRQALPQQPRTAEQLAAVARGGYTLIDTEGVPEAIVIACGSEVGIAA
                     DAVRALQAQGRRVRLVSMPSSGAFDAQDAAWREAVLPRAVTRRLAVEAAARQSWWRYV
                     GSDGEVIGIDSFGASGKAADLSRHFGITADHIVAAVGKLLA"
     gene            24933..26111
                     /gene="metK"
                     /locus_tag="PKN91_02075"
     CDS             24933..26111
                     /gene="metK"
                     /locus_tag="PKN91_02075"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNS26886.1"
                     /translation="MPNSYLFTSESVSEGHPDKIADQISDAVLDEILTQDPRGRVACE
                     TLIKTGVVIVAGEVTTSAWVDLEALVRRTVLGIGYNHSDMGFDGATCGVLNVIGKQSA
                     DIAQGVDRKDERKQGAGDQGLMFGYASNETDVLMPAPITYAHRLVQRQAQMRKRGKLR
                     WLRPDAKSQVSLRYEDNKPVAIDAVVLSTQHSPDVSTKTLREAVMDEVILPVLPKKWL
                     HKGTKFHINPTGRFVIGGPVGDCGLTGRKIIVDTYGGFARHGGGAFSGKDPSKVDRSA
                     AYACRYVAKNIVAAGLADRCEVQVSYAIGVAEPTSIMVETFGTGKLPREKLTQLVRRF
                     FDLTPYGLREMLDLTRPIYLKTAAYGHFGREDADFTWERTDKAAALAAELRRTRARAA
                     "
     regulatory      26160..26241
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            26256..27677
                     /gene="ahcY"
                     /locus_tag="PKN91_02080"
     CDS             26256..27677
                     /gene="ahcY"
                     /locus_tag="PKN91_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNS26887.1"
                     /translation="MTAVAKPSGTTPDYFVRDIREADWGRKEMAIAETEMPGLMAIRK
                     EFGPVLPLRGARIAGSLHMTIQTAMLIDTLKHLGADVRWASCNIYSTQDHAAAAIAAG
                     GTPVFAYKGETLDEYWQFTHAIFDWPGNQYANMILDDGGDATLLLLLGAQAEKDPSVI
                     AKPTNEEEVALFAAIRARLGQSPDWYSKRLAHIKGVTEETTTGVKRLYQLQRDGKLPF
                     PAINVNDSVTKSKFDNLYGCRESLVDAIKRATDVMVAGKVAVVAGYGDVGKGSAESLR
                     ALSAQVWITEIDPICALQAAMEGFKVVTMDEACDKADIFVTATGNLNVITHEHMRRMK
                     HQAIVCNIGHFDSEIEIAALKQYKWEQVKPQVDHVIFPDGKRLIVLAEGRLVNLGCGT
                     GHPSFVMSNSFTNQVLAQIELFAHGDKYGKEVYVLPKHLDEKVAQLHLERIGAKLTTL
                     TPEQAAYIGVAPQGPFKPEHYRY"
     gene            27920..29731
                     /locus_tag="PKN91_02085"
     CDS             27920..29731
                     /locus_tag="PKN91_02085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNS26888.1"
                     /translation="MQESGSTAQAQAHAPAEIPGGHSRKTRKDVIVFTSDDEFLLSLG
                     PAMDDRFRSHTTDGTGAWAEMVRGRAGVALIDAATLANAPEIVRTMEAEFPSFALVVV
                     APAAARPQWTLALTRGAIMQLLERENLETNAIAAALTAEPRVASAGAATASGAAGDGS
                     TPRKLPLPMIAAAAAAVLVLAGAAWLWLGGDDSSPATIADEPAATTATPVAEPAEAPA
                     QADAAPADTRSVPELLSAARIAFAERRYVEPATGNALALYSRVLAVEPANAEAIDGVQ
                     RIVAIVAGQAQAQIKAGQIDEAAKLLDVLRVAAPNDSALAAVEADMAAARPKWLAARA
                     REAISNDQFQGAERLIDELAALGTEKALVQDLRRSIDAQRRNADLARAVAEARASLTP
                     ALLLDTSARGPSAQLASLQQIDRRHSQVVAFQRDYLAALTRAARDATGSGDFAGAERY
                     LAAATKAGGSRDATDARKELQAARDAAAAREQQRAETAQRTARSPAPAAGAAAAGTAA
                     TAPPPRMPKAKKRGAPKYPAQAERDGIEGEVVVGFALTPEGRPRDLQVIESTPPGVFD
                     AAAMDAVRGWRYEQISAADAERLPRASVRLTFRIGDR"
     gene            29728..31887
                     /locus_tag="PKN91_02090"
     CDS             29728..31887
                     /locus_tag="PKN91_02090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS26889.1"
                     /translation="MMKRRRTDFDLAQLMPVVVATTFAAVLTLLLTLGIQRASQLQSA
                     SSALQLASELRSLPQYFGTELTLVQRGLESRTFIGEPLADIAAAREGFDKALERIDAD
                     LIAAGLGGTVEIQRVRGALDAAWARLSPLLVAVDSHKGTPYSDTLSGSRLNATGRALK
                     ADVDAALAAQDDSMRDVGGALKDLATGLQDAVGDRGGSLRSFLLIGAAVSTLLLALML
                     YFALRSRRATDDARRAERQVADILGTVREGLFLLDRNLALGAVYSASLTRILHTENVR
                     ATSFEDLLRPLVPAKTLATAMKFVNLLWKEKVHEDLIDTVNPLNQIEVQFPKSGGGTD
                     SRFLSFAFKRVRNAGGQGEHLLGIVTDVTDRVQISRELEQSKAENQAQLDLVMQLLQV
                     PGAQLQSFVRDADMALRKCNAILKQPGREEAELRSKLNGVFREVHTIKGEAGALSLTS
                     VASRVHQLEESLVALRDRAQIDGNDFIPIVVKLDELMSYLGGVGQFADRLSEVQPKGD
                     TMKSVVPPLLANQLLTSEEPTLEQVLAKVVKDTAQAHGKRVALQCRGLEAIPPAYRKA
                     ARDITIHMARNAVVHGIETPDHRVSASKSETAAVRVSFDEAGADGYVLTVEDDGQGLP
                     YERILDMALRKGLVSPADAARMERPAVYGLIFRAGFSTADKVTEHAGRGVGLDAVSQT
                     VRDLGGSIGIATADGKFTRFRMKLPQAAVQAGASHVA"
     gene            31899..32276
                     /locus_tag="PKN91_02095"
     CDS             31899..32276
                     /locus_tag="PKN91_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS26890.1"
                     /translation="MNAVRQHRLMIVDDSNIMRRKIERSQDIPQVEIVGLAGNGREAL
                     ELHEQTRPTLVTMDLTMPEMDGIECVEQLVGRDPDIRILVISALADKATAIEALEKGA
                     SGFLCKPFTERQLNDALVKLIES"
     gene            32286..32735
                     /locus_tag="PKN91_02100"
     CDS             32286..32735
                     /locus_tag="PKN91_02100"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNS26891.1"
                     /translation="MSLQGEIGTFVAAVLNYFDTAVQQPAVVGTPYLALGKAPEVFDY
                     TGMIRISGKRKGVVYFTAPKGMLSVMLMRMQETDMSDANLRDLVGEVANTLSGNARRD
                     FGRNFIISTPSVVAGSSTTIGYSPDTRPIVVPIDWRAYHANLVVCLN"
     gene            complement(32745..>33373)
                     /locus_tag="PKN91_02105"
     CDS             complement(32745..>33373)
                     /locus_tag="PKN91_02105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS26892.1"
                     /translation="QRYLIEHTVREPAILAALRQLTAKMPQANMQIAPEQGQLIQLLV
                     RLIGARRCLEIGTFTGYSALCVALAMPRDGELIACDVSLEWTQIARRFWREAGVDARI
                     DLRIAPALTTLDELLQGGGSSFDFAFLDADKENYLEYYERVVELLRPGGLLVVDNVLW
                     AGAVADAGVQDAETNAIRALNDRARDDERVDMSLVPIGDGLLLARKRG"
BASE COUNT         5325 a        11687 c        11263 g         4998 t
ORIGIN      
        1 ccagcagggc gagcgaaccg aagaaatacc agaagttgaa gttcttcggc gcgtaatagc
       61 cgacgagctg gctctgcatg aactcgtcga cgggcagccg cttgttgagc cacgcgcgga
      121 acccgccctt gccggcggcg gcggtagtgt cgttcgccat cacgcaactc cttcctgcgg
      181 gtcgacgccg atgacgagcg tgtcgccgcg aaatgcatgc ggcggcacga cgaggttggt
      241 cgaagccggc gaaccggaca tcacgcggcc cgcgaggtcg aacttcgagc catggcacgg
      301 acagaagaac ccacccggcc agtcggcgcc gagcgcggct tcaccgcgct cgaaatgctg
      361 cttcggcagg cagccgaggt gggtgcagat gccgacgatg acgaggaatt cgggcttcgc
      421 cgagcgcaat gtgttctttg cgtactcggg ctggatcgac gcgtcggaag ccgggtcttt
      481 cagcatcgac tcgtccggcc cgatcgccgc gaccatctcc ggcgtgcggt gtacgacgta
      541 gatcgcctgc ttgcgccagc tgacgatgac catctggccg ggctcgattt tggtgacatc
      601 cacctcggcc ggagcaccca gcgcgcgtgc gcgctcggag ggcttccacg acttgatgaa
      661 cggcacgagg gcgaagccga cgcccacggc gccggtcccc acggtggcgg cggtcaggaa
      721 ccgcctccgg ctatgatcga tctcctgatc ggcggtcgcc gcaatatcgg tcatcgaaga
      781 gtctcccaac gtacagcccg tgcctgcgcg ccctgcagaa tgccaggttt gtgacgcgca
      841 ccgggaagcg gcgcattata cacacgcatg accccgcaaa gacttggccg cgcattggta
      901 tttctcgcag gttcggtcat cgggggcctt gcgctcgcct tcgtgatcgt cttcctgcga
      961 cccgacctgc tgcgcgcgcc cccgccaccc gccccggccg cgccggcccc ggccatcacg
     1021 gcgccgcccg cccctgtcgt cagctacgcc gacgccgtgg cgcgcgccgc gccggcggtc
     1081 gtgaacatct actccgagcg cgtggtcaaa gagcgcttgc cgccggcgac gctcggcgaa
     1141 ctgttcggcg gcgtcatgcc gcgctaccgc cagcgcatcg aacagaccct cggctccggg
     1201 gtcgtggtcg acgccgccgg gcacatcgtc accaaccacc acgtgatcgc ccaggccgaa
     1261 cgcgtcagcg tgcaactcgc ggacgggcgc cagggcgaag cgagcgtggt cgggcgcgac
     1321 ccggataccg acctcgcggt cctgcgggtc gacctcggcg aggtgccggt gatgccgctc
     1381 gggcgctccg accaactgcg cgtcggcgac gtggtgctcg cgatcggcaa tcccatcgga
     1441 ctcacccagt cggtcaccca gggcatcgtc agcggcaccg gccgcggcca gctcggcgtc
     1501 gcgaccttcg agaacttcat ccagaccgat gcgccgatca acgtcggcaa ctccggcggc
     1561 gcgctcgtga acacgcacgg agagctggtc ggcatcaaca cggccgtgct cgcgaagaac
     1621 cttggcgtag agggcatcgg gtttgcgatc ccggtgaacc tcgtgcgcgg ggtggtgcag
     1681 gagatactgt cccacggccg ggtgatccgc ggctcgatcg gcgtggtgct cggcgaagtc
     1741 gacgcggcac aggccgaacg gctcggcctg tcacgcgcgg gcgtcgtgat cacgaacctc
     1801 ttccagggca gtcccgcgct cgaggccggc ctgcggcccg gcgacatcgt cgagagcatc
     1861 gccggtaacg cggtgcgcgg ccagcaggac gcgctgtcgc gcatcgcgac ggcgcggccc
     1921 ggcgagaccg tcgatgtggt gatcgttcgc ggcaccgaga cgatcacggt tccggtcaag
     1981 gtgacggagc ggcgtacgtc gtagtgcagc ggcggcgcgc ggctgcggcc acaccttcgc
     2041 ggtcacgcct ccgccgcttc gcggccacag tcacacgtcc gccgcctcgc ggctgcggcc
     2101 acacgtccgc cgcttcgcgg ccgcggttac acgtccgccg cctcgcgggt cgagcgcacc
     2161 ggctaccgcc ggtgtgccct cgtcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnngagagg agacggtcat gcatcggcct cgcccgcacg cccggcgccc ccgcgcgccc
     2341 gcacaggatg cgcgggtggg gccgatgcgt gaccgtcgac gaacgtggag cgcgccgagg
     2401 cgcggcggtc agagcgaggc gactaccgag ggccctgctg ccaccgacac gatgcgccga
     2461 gcgccgccgc accgagggca ccgcttcgtc agaagcggcg cgcgacccga gaggagacgg
     2521 tcatgcatcg gcctcgcccg cacgcccggc gcccccgcgc gccgtgcgct tcatcccggt
     2581 acgcgccgga tctgcgcccc cagctgcgag agcttctcct cgatcgtctc gtagccgcga
     2641 tcgatgtggt agatgcgctc gatgtcggtg cgcccctcgg cgacgagtcc ggcgagcacg
     2701 aggctggccg aggcacgcag gtcggtcgcc atgaccggcg cggcggtgag tttcggcacg
     2761 ccgcggatga tcgcagtgtt gccctcgagg cggatctcgg cgccgagccg gcgcatctcc
     2821 agcatgtgca tgaaccggtt ctcgaagatc gtctcgatga tcgtgccgac gccgtcggcg
     2881 atcgtatcga gcgccgcgaa ctgcgcctgc atgtcggtcg ggaagcccgg atagggcgcg
     2941 gtgcggatgt cgaccgattt cggccgcttg ccgcgcatgt cgatttcgat ccagttctcg
     3001 cccacgtcga tcctggcgcc cgcctcgacc aacttgccaa gcacggcatc caggtgatcg
     3061 ggccgcgtgt tcttgatgcg cacgtggccg ctggtgatgg cgcccgcgac gaggtaggtg
     3121 ccgctttcga tgcggtcggg cagcacgaca tagtgcgcgc cgcccaggcg ctcgacgccc
     3181 tcgaccacga tggtgtcggt gccggcaccg cgcaccttgc cgccgagcgc attgacgaag
     3241 ttcgcgagat cgacgacttc gggctcgcgc gccgcgttct cgatcacggt ctcgccctcg
     3301 gccagcgccg cggccatcat caggttctcg gtcccggtca ccgtgacggc ctcgagcacc
     3361 agccgtgcgc ccttcaggcg cgaggcgcgt gcacggatgt agccgccctc gatgctgatg
     3421 tcggccccca tcgcctgcag gcccgcgacg tggatgttca ccgggcgcgc gccgatcgcg
     3481 cagccgcccg gcagcgagac atccgcgcga ccgtggcgcg cgagcagtgg cccgagcacg
     3541 aggatcgagg cgcgcatcgt cttgacgagt tcgtacggcg cgtagcattc gcgcagcgtg
     3601 gtcggatcga cctcgacatg catgcgttcg ccgatcgtga catcgacgcc catgcggccg
     3661 agcagttcga tcatggtcgt cacgtcctgc aggtgcggga cgttggccac ggtcacgggg
     3721 ccgtcggcca gcagcgtgcc cgcgaggatc ggcagcgcgg cgttcttggc gccggaaatg
     3781 cggatctcgc cctcgagggg cacgccgccc tggatctgca gcttgtccat tcagcccgcg
     3841 gcgcgcgcgg cggcctcttc cggagtcagc gcgtcgatcg acagcgcgtg aatttcgccg
     3901 cccatgcgct cgccgagcgc ggcgtagacc atctggtggc gcgcgatgcc gcgcttgccg
     3961 gcgaagccgg gagcgatcac cagcgccgca aaatgcacgt tgtcctccga ctgcacgtgg
     4021 acctgggcac cgggcagggc ggcccggatc agttcggcga cttcgctggg ttgcatccgg
     4081 caatactaaa gtgttcatgg ccgtggcggt atcattcggg catgaacgcc gcaacggacg
     4141 cacccggatc gcccgccacc accgatgccg gactgctcgc cgccggccgc cgcgcgctgc
     4201 gcatcgaggc gaccgcggtc gaatcgcttc tcgagcgcct cgacgcccgc tttgcggcgg
     4261 cctgccgcct gtgcctcgcc tgcgcggggc gcatcgtcgt cacgggcatg ggcaaatcgg
     4321 ggcatatcgg cggcaagatc gccgccacac tcgcgagtac cggctcgccc tccttcttcc
     4381 tgcacccggc cgaggcaagc cacggcgacc tcggcatgat cacccgcggt gacgtcgtgg
     4441 tggcgatctc gaactccggc gagacgcccg aggtcgtgac gctgctgcct cacttgtcgc
     4501 gcctcggcgt gccactgatc gcgatgaccg gcgccacgga ctcgaccctc gcgcgcgccg
     4561 cggccgtcgt gctcgacatc agcgtgccgg aggaagcctg cccgctaaac ctcgcgccca
     4621 ccgccagcac caccgtgacc ctcgcgatgg gcgatgcgct cgccgttgcc atcctcgatg
     4681 cgcgcggttt caccgagcag gacttcgcgc gctcgcatcc cggtggcgcg ctgggccgcc
     4741 ggctgctgct gcatgtcgag gacgtgatgc gcaggggcga cgacatcccg cgcgtcgcgc
     4801 ccgatgcgct gctgaaggag ggcctcgtcg agatgtcccg caagggcctc ggcctcaccg
     4861 tgatcgtcga cgagtcgggc aggagcctcg gcgtgttcac cgacggcgac ctgcgccgca
     4921 tcctcgacca gccggtcgac gtgcagcgca cgcgcatggc ggaggtgatg acctcgcccg
     4981 gcaaggcgat cgggccgcgc gaactcgccg ccgaagcggt ccacatgatg gagttgcaca
     5041 ggattaccgc gctgccggtg atcgacacgg cgggccgtct gatcggcgca ctgaacgtgc
     5101 acgacctgct gcgcgcgggg gtcatgtagc catggtccgc gccaccacgc tgccggccgc
     5161 aatccgcctg ctggtgctcg acgtcgatgg cgtgctgacc gacgggcgcc tctactacgg
     5221 cccgcgcggt gaagtgctca aagtcttcca cgtgctcgat ggccatggca tccgcgaagc
     5281 gcagcgcgcg ggcatcgaag ttgccgtgat atcgggccgc gacagcgcgg ccgtgcgccg
     5341 ccggtgccgc gacctcggga tccgccacgt cttccagggc gtcgccgaca agctgccggt
     5401 attcgagcgc ctgtgccggc ggctgcgcat cctgccgatc gaggcggcga tcatcggcga
     5461 tgacgtgccc gacgtgccga tgatgcaggt cgtcggcctc gcattcgccg tccccgatgc
     5521 acaccaggcg gcgcggcgcg ctgccgacca caccaccagc gcacgcggcg gtcgcggcgc
     5581 ggtacgcgaa gtgtgcgacc tgttatgtgc ggcctcggcg cgccggccgc gcaagagcgc
     5641 ccggaagcgc cgatgacccg ctggctcatc ggcttcgcgg tcatcgtgac ggtcatcgct
     5701 tcgatcgtgc tcagccgctc ggcgcgagcg cccgacggca tcggcgccag cctgccgacg
     5761 gccgccgatc ccggctacct ggcccgcgat gccgaagtga tcgagaccgg cgatgacggc
     5821 cggccgcgct accgcctcga cgcagaactg atcgagcagg cgccgaacga cccgacgatc
     5881 acgctctcgc gaccggtgat gcactacgcc gatcccaggg gcggcacctg gcgcgcgagc
     5941 gccaaccagg gcaccgtggc gccgccaagc gacgtggtcg aactcaccgg cgatgtgctg
     6001 ctgaccggca ccctggcgga cgggggcgac ccggcgcacc tcacgaccaa ccggctgacc
     6061 ttcgacgccg ggcgggacgt cgcgcacacg agcgccccgg tcacgatcga gtggtccggg
     6121 caccggctca ccgcgcgtgg cgtgcgcgcg gacctcgggg ccgagaccct tcggctagaa
     6181 tcggcggtcc atggcaattt cctgcccccc tgaccgacgc cgtccggcgc gcctgctggc
     6241 cgggctcgcg ctggcggccc tgtgcgccgc acgcacgatg ccggcggccg aacctgaccc
     6301 gcgcaccctg ccgatcaacg tcgatgccgc ctcctccgac gtcgattacc gcagcaatac
     6361 ggtcgtgttc cgggatgtcg tcatcaccca gggcggcgcg cgcgtcgctg ccgcgaacgc
     6421 gcgcgctacc ggactcgatt tcgtggattc gacctgggag ttcaccggcc gggtgcgcat
     6481 cgatcgcgac ggcggcaccc tcgaatcgga ccgggcgaaa gtgcggtttc gcgacaacgt
     6541 gctggaaagc gcgacgatcg agggcaagcc ggcccgcttc gagcagcagc tcgtccgcac
     6601 gcccggcaag gcgcacggcc gcgccgagac gatcgtctac gacatggcgg cgaaccgggt
     6661 caccttcacc ggcgatgcct ggctgaccga tggtcgcaac gagatcagcg gccaggaact
     6721 cgtctacaac gtcgccgacc agcgcgtgct cgccgaaacc cagcccgggg acaccgaccg
     6781 cgtgcggatc acgatccggc cccagaagga tgccacgccc gcggacgagc cggctccatg
     6841 agccggttgc gggtggaagg cctggccaag cgctacggca aacgatcggt catcacggat
     6901 ctgtcgctcg aggtgtcgag cggcgaggtg ctgggcctgc tcggcccgaa cggcgccggc
     6961 aagactaccg ccttctacat ggtcgtcggt ctcgtgccgg cggatgccgg ccacatctgg
     7021 ttcgacgaca ccgacatgac gcacatgccg atgcacaggc gcgcgcggct cggcgtcggc
     7081 tacctgccgc aggaagcgtc ggtgttccgc aagctctccg tgcaggacaa cgtgctcgcg
     7141 atcctcgaga cacgcgccga cctcgatcgc gccggtcgcg aagcgcggct ggaggaaatc
     7201 ctcgaggaac tgcgcatcgc gcacgtgcgc gacagcctcg gcatgtcgct ttcgggcggc
     7261 gagcgccgcc gcgtcgaaat cgcgcgtgca ctggccgcgg aaccgcgctt catgctgctc
     7321 gatgaaccgt tcgcgggggt cgacccgctg tcggtgctcg acatccagcg catcatccgc
     7381 gagctcaccg ccaagggcat cggcgtcctg atcaccgacc ataatgtcag ggaaacgctc
     7441 ggcgtctgcg accgcgcctg catcgtcagc tcgggcaccg tgatcgctgc cggaaatgcc
     7501 gaagaaatcc ttgccaatgc ccaagtccgt gaggtgtact tgggcgaaac cttccgtctc
     7561 tgaccggtgg tcgctatcct caatattctt tccgaatcaa cctgttgggc gtacccccct
     7621 ccgccatgct gaagccctcc ctgcagctca ggctgggtca gtcgctgacc atgactccgc
     7681 agttgcagca ggccatccgg ctgctgcagc tgcccgcgct cgagctgcag gcacacatcc
     7741 gcgagctgct cgagtcgaac gtcatgctcg aggcggtcga ggaacccgag cagaccggca
     7801 ttttcgaggc gatccagtcg cccgccgaac gcgtggcgcc gcagcgcgag gagccccgtg
     7861 tcgacgtcga ggtcatcacc gacaactggt cgggcgagtc ggcgggcggc tccgattcgc
     7921 cgtggtcacc cgatgacgac gatcgccagc aggactacgc cgctcagtcg gacacatcgc
     7981 tccaggatca cctgctcgcg cagctcgaag tcgccaatct cgatgcccgc gacctcgcga
     8041 tcgcgcgcgc catagtcgat gccgtgaacg atgacggcta cctgttcgag ccggtggccg
     8101 agatcgccgc gacgctgcgc ccggacgtcg agtgcacgga cgaggaggta ctcgcggtgc
     8161 tcgcggctgt gcagactctc gacccggcgg gtgtcggcgc gcgctcggtc gccgaatgca
     8221 tcacgctgca gctgcgccag ctcgatgccg acacgccggg cctcgccacc gcgctcgaaa
     8281 tcgccgcgcg gcacctggac gcggtcgcca agaacgaact cgcactgttg cgccgcgaac
     8341 tacgcgtcac cgaggacgaa ctcgccgagg ccctggctct cgtacgcacc tgccagccgc
     8401 ggcccggatc cgcggtcggc agcggctcgc cggagttcgt ggtgcccgac gtattcgtgc
     8461 gtcgcacgga gcgcggctgg agcgtcgaga tcaatccgtc cacgctgccg cgggtgcgcc
     8521 tgaaccaggg gtacgcgagc gcgctcggcc gcaattcctc gcatgcgacg ctgcgcgcgc
     8581 agctgcagga agcgcgctgg ctgctgaaaa gcctcgagat caggaacgac acgctgctca
     8641 aggtcgcgcg cgccatcgtc gagcgccagg ccgatttcct cgagcacggc gaggagcaca
     8701 tgcggccgat gatcctgaag gacatcgccg cggccatcga gatgcacgaa tcgacgattt
     8761 cgcgcatcac ctcgggcaag tacatgcaca cgccgcgcgg cgtgttcgag ctgcgctact
     8821 tcttctcgag ccatgtcggc ggcggcgagg ctgcggaaac ctcctcgact gcgatccgcg
     8881 ccaagatcag gaaactgatc cgcgacgagg accccgccgc accgctgtcg gacagtcgca
     8941 tcgccgaact gctgtccgcc gaaggtattc ccgtcgcgcg ccgcacggtc gccaagtacc
     9001 gcgaagcgat gcgcatcgcg ccgtcgaacg aaagacggcg cgcagcgtcg aaatgatgat
     9061 ccaggagagc accatgcaag tcaccgtcac cggccaccat gtcgaggtca ccccgccgct
     9121 caagagctac atcgagaaaa aactcgagcg catcgtgcgc cactacgaca gcgtcatcga
     9181 cgtgcattgc gtgctgaccg tcgagaagca gcgccagaag gccgaggcca cgctgcagct
     9241 gcgcggcggc tcgatccatg ccgatgcgat cgacgaggac atgtacgccg cgatcgacgc
     9301 actcgccgac aagctcgatc gcctggtccg caagcacaag gagaagctgc aggatcacca
     9361 cgctgccgag gctcgcaagg ccacccggca atagtcgcac caccgcagtc tcgcgccatg
     9421 cgcctcgtgg tcgtcagcgg gctgtccgga tccggcaaga gcgtggcgct gcacatgctc
     9481 gaggacctcg gttactactg catcgacaac atcccggccg cgctgctgac tccgctgatt
     9541 tcgcacacgg tgcgcagcgc cgaggcggcc tacgagcgca tcggcgtcgg actcgacgcg
     9601 cgcaacacgg cggcggaaat cgccaccgta ccgaagctga tcgccgaact gaagaagagc
     9661 ggcatccagt gcgaggtgct gttcctcacg accagcgacg acgagctgct gcgccgcttc
     9721 gccgagacgc gccgcaagca cccgatgagc cgcaacgaca tcggcctgcg cgaggcgatc
     9781 gcgctcgagc gtgacctgct ggaacccatg atgtatgcgg ccgaccaggt gatcgatacc
     9841 tcgcgcatgg gcgtgcacgc gctgcgcgac agggtgctga aatcggctga tgagcgagcc
     9901 acaccgcgcc tcgcgatcac gttcgagtcc ttcggcttca agcgcggcat cccgggggat
     9961 gccgacttcg tattcgacgc gcgctcgctg cccaacccct actgggaacc acagctgcgc
    10021 ccgctgaccg gccaggaccc ggaagtgatc cgcttcctcg aggccgagcc tgccgttgcg
    10081 cggctgatcg aggacatcgc gaacttcgtg gaagcgcgca tccccgagca caccgcggcc
    10141 aaccgccgct acctgaccgt tgcgatcggc tgcaccggcg gccagcatcg ctcggtctac
    10201 atcgcgaacc aactggccgg acgattttcg gcgcatcatc cgcatgtctc cacccgccac
    10261 aacgaattgc gtgagcggcc gggggcgcac tgaatgggag gactgatgga catgtccgca
    10321 ttgcgaaatc tcgctggcct cgcgctcggc gcgctgctca ccagcgcctg caaccaggac
    10381 gcaaaccccg ccatggccag cgtctccggc accgccacct atcgggagcg catggcgctg
    10441 ccgccgggcg ccgtattcga ggtgcgactc gaggacgtct cgcgggccgg cgccgccgcc
    10501 gggatcctcg ccgagacgcg cgtcgaatcg cccggcaatc cgccgttcgc ctttacgctc
    10561 agctacgatc cggcgcacat cgatgccacg cacagctatt cgatccgcgc gcgcgtgctc
    10621 catggcgacg ccgtgctgtt cacctccgat acgcatacac cgctgccggc ggacgactcg
    10681 tccgcaccgc tcgagatcct gatggtgagg gccggcgccg cgcgggcggg gagtcccgac
    10741 gtgcccgatg ccacccccgg acccgggtta ttgcgcggca cgtacagcta catggccgat
    10801 gccggcctct tcacggcctg cggcaccgat gaacggatac cggtggccat ggagggcgac
    10861 aacgccgcgc tggagtcagg ctaccggaag gtggtgccgg agccgggcgc caccctggtc
    10921 gccacggtga tcgggcgcat cgaagctcgc gaacccatgg agggacccgt gcgcccgcac
    10981 ctgatcgtcg agcagttcgt gaacgtcgtc gcggggacct gcgaaacgcc gccggtcgtc
    11041 gcactcgaag atacctactg gcagctcgcg cagctcggcg gcacgcccgt cgctctggcc
    11101 gccgggcagc gtgcgcccta tttcgtgctg gactccgcca ggcatcgcgt gagcggctac
    11161 gccggctgca accgcatgat gggcggctac agcgtcgacg gcgcgaagct cgccttcacg
    11221 cagatggccg gcaccatgat ggcctgcctg cagggcatgg agatcgagca ggccctgcac
    11281 gaggcgctga agcgtgtcac cgcctggcgg atcgagggca atcgcctcga tctgctcgat
    11341 gcggacggcg cagtcgctgc ggcgttcact gccgtgtagg cctcgcagtc gccgtgtcgc
    11401 gcgtcgtcac cggctgctag actccgcgcg cccgcgcggg gcccgcctgg atagccgtcg
    11461 gtcatgagcg acaccgatac cacaccactg aagtctggac gcctgtcggc gctgcgggac
    11521 gcgctcgacc agggcacgct gcgcgccgcg caccgcatgg tcaatgcgat gcaccccgcc
    11581 gatatcgccc tgctgctcga atcgctgccg cccgcgcagc gcgagatcgt ctgggaaatg
    11641 gtcgatccag agctcgaggg cgacgtgctc gtcgagctga acgagaacgt gcgcgccggg
    11701 ttcatcgagg agatgggcgc cgaggaactg gtcgccgcga ccgaaggcct cgaggtcgac
    11761 gatctcgccg acctgctcga tgagctgccc gagaccgtca cgcagcagct gctgcactcg
    11821 atggaccagc gcgaccggga gcggctgcgc acggtgctcg gctatccgga ggacagcgcc
    11881 gggggcctga tgaacaccga cacggtcagc gtgcgcgccg acgtgccgat cgaagtggtg
    11941 ctgcgctacc tgcgcatgcg cggcgagctg ccggaaggta ccgacagcct gttcgtggcc
    12001 gaccggcacg accgctacct gggctccgtg ccgctgaccc ggttgctgac ggcggatccg
    12061 gaccacctgg tcggcgattg tctcgacacc gaggcgccgc gcatcgaccc ggacatgcac
    12121 gcgcacgacg tctcgcggct gttcgaagac cgcgacctgg tatccgcggc ggtcgtgggt
    12181 tccgagggca agctgctcgg ccgcatcacc gtcgacgaca tcctcgacgt gatccgcgag
    12241 gaagccgagc actcggtcat gtcgatggcc ggcctcgacg aggaagaaga cgtcttcgca
    12301 ccggtgttgc cgtccgcgcg ccgccgcctc gtgtggctcg cgatcaacat catgatggcc
    12361 ttcatcggtg ccttcgtcgt cgcgagcttc gaggacacga tcgaaaaggt cgccgtgctc
    12421 gcggcgctgc tgccggtggt ctcgagcatg ggtggcatcg ccggcacgca gacggtcacg
    12481 ctgctgatcc gcggcctggc actcggcaag gtcgattgga ccaacgcacg ctggctgcta
    12541 ttcaaggaaa tcgcggtcgg cggcctgaac ggcatcgtgg tcgcatcgac cgtggccctc
    12601 gtcacgctgg cgctgatcgg ccggtggcag atcgcggcga tcgtcgccgc cgccatgctg
    12661 atgaacctgc tggccgcagc gaccgtcggg gtgctgattc cgctcgcgtt gcgtcgcctg
    12721 cgcatcgacc ccgcgctctc tgccggcgtg atcctcacca ccttcaccga ctgcatcggg
    12781 tttgcgacgc tgctcggcct cggcgccctg ttcctggtct agcccctcag ccgagcagga
    12841 aggcgcgcag tcggtcgcgc tgctgcacgg catccccata gccgagttcg atgagctccg
    12901 aggtgtagcc ggactcgaac atcaggtaac tcgcgagctg gtggccagcg acgccgcgcg
    12961 caccgatcac gcgcaggaac gcgcgcaggc tgcgcggcaa ctcgtgcagg tggcgcgcgg
    13021 cgatctcgcg cgggtcgata ctcggcgaaa tcgccatcgc ctcgatcagc cgcacgtcgg
    13081 gcacggcctc gggcgcggta cgcgccatgg cgttgaggcg aatcagctgc tcgaggtcgg
    13141 cgtaaatgcc gtcggtgaac agcgtgtcga gcatgaagcc gaacagctga ccgggcgtcg
    13201 gtgagcgcgg aagctgcgac acctggctca ggcccgccgc atttgccgca cgcacgccga
    13261 tcacgagcac ccgttcggcg ccgaggtgca cggccgggct cattggcgcg agctgacgca
    13321 tcgcgccatc gccgaaatac tcggtgccga gttccacggg cggaaacagg aacggcacgg
    13381 cgagactgcc catcaggtgc gacagcgtga ggtcgacgcg gcggccctcg cggtgcgccc
    13441 gcgcccattc ctggatctcc tccgcgccgt cgaagaacgc caccgagtgc gcgctggtgt
    13501 agccggtcga gcacagcgcg acggcacgca gcatgccggt ggagacgctg tggcggatgg
    13561 cgccgaagtc gatgttgcgt ccgagtagct ccgcaagcgg cgcattgtcg aagatcgacc
    13621 tgggcgggtt cgcgagcatg ccgcccgaca cgagcgccac gagccagcgc agacccgacg
    13681 ccagcatgtg caccgagccg acgcgaaaca cgtggtcgct gtggaaggag gcccacacat
    13741 gttcgagcga ggcgacggcg ccgcgccagc gccttgcctc ggcggcgagc gccgccgtgg
    13801 cgaccgcacc ggccgaagtc ccgaccagca ccgggaacgg gattccgcgg cgcggcagca
    13861 tctcggacag cgcccgcagc acaccgacct ggtaggccga gcgcgcgccg ccgcccgtca
    13921 ggatcagggc ctgtacgggg cgtccggaaa tgatggtgtc ttgccgcatc acgcgcgtat
    13981 tctggcgcat gccccgcggc tggtagaatc gatcagttcg cgccccagca tcccgacaat
    14041 cgtggagaag tccatgctac ccgatcgttc gacactgctt gccgccatct tggcgatggc
    14101 cgccgtggcc atgcccgcac tcgcccagga ttcgcccgcg gtcggatccg cggccccgac
    14161 cttcaagctg caggaccaga atggtgcctg gcgcaccctc gaggagcagc gcggcaaatg
    14221 ggtcgtgctg tacttctacc cgaaggacaa cacgcccggc tgcaccacgc aggcctgcga
    14281 attccgtgac aacatctttg cgttccgcga agccggcgcc gtgatctacg gcatcagcgt
    14341 cgatgacgtg aagtcgcacg aggaattcgc cagggagcac agcctgccgt tcacgatcct
    14401 cgccgactcc gccaaggaga ccgcgaagag ctacggcgtg ctctacaagg cgatgggcct
    14461 gctcgagctg gcgcggcgcg ataccttcat catcgatccg caaggccgca tcgccaagca
    14521 ctacgtgaag gtcgatccga agggccattc cgagctcgtg ctcgccgaca tcaaggcgct
    14581 gcaggcggcg gcgaagggct gatccccgcc gccgctggcg gcggctgcgg cggcatgctc
    14641 ccgcgccgcc gcagctcggc tgtcgcgcgg tccagccccg cgagcgtcag cgggtacatc
    14701 cgcccttcga ccagttcgcg cgtgatcacg gtcgatggcg tataggccga gcgtttctcg
    14761 ggctccgggt tcaaccacac gaagtggcgg aaatgcgcgg tcaggcggcg cagccaggtc
    14821 gcgcccgcct ccgcattcca gtgttccacg ctgccgcccg gcatcgtgat ctcgtaggga
    14881 ctcatcgccg catcgccgac gaagatcagc cggtagtccg ggccataggt ccggatcaac
    14941 tggtcggtcg ggatgcgttc ggtgtggcgg cggcggttgt ccttccacag cgactcgtac
    15001 gggaagttgt ggaaatagta atgctcgaag tggcggaact cgctgcgcgc ggctgagaac
    15061 agctcgtcgc agacccgcac gtgctcgtcc atcgagccgc cgacgtcgag cagcagcagc
    15121 actttcaccg cgttgcgccg ctcgggcacg attttcaggt cgagatggcc ggcgttgcgc
    15181 gccgtcgccg aaatcgtgcc atcgagatcg agttcctcgg ccgcgccctc acgggcaaaa
    15241 cggcgcagcc ggcgcagcgc catcttgaag ttgcgcgtgc cgagttcgag cgagtcgtcg
    15301 aggttcgcga actcgcgccg atcccacacc ttcaccgcgc gccggtgccg cgacccgccc
    15361 tgcccgatgc gcacgccctc ggggttgtag ccgcctgcgc cgaagggcga agtgccgccg
    15421 gtgccgatcc acttgttgcc gccctcgtgg cgttcgcgtt gttcggcgag gcgctgccgc
    15481 agcgtctcca tcagtttctg ccaaccgccg agcgcctcga cctgcgcctt ctcctgctcc
    15541 gtgagcacgc gctcggcgag cgagcgcagc cattcgtcgg gcactgacgc cgtgatcgcc
    15601 tcgaacgcct tctcgatacc cttgaagtgc tcggcgaaca cgcgatcgaa gcggtcgtag
    15661 tggcgctcgt ccttcacgag acacacgcgc gccaggaaat agaagtcctc gacggacagc
    15721 caggcgacgc gcgcctgcag tgcctcgagc agggccagga actccggcag cgtggccggc
    15781 acgccggcgg cgcgcagctc gccgaagaac ctaacgagca cgccgggaga ggaacatgac
    15841 ctgctcgacg aggtgcatgt cctgctcgtt cttgaggagc gccccgtaca gcggcggtag
    15901 tgtcttcttc gcgtcgtcgc cacgcagcgc ctcgggcggg atgtcctcgg cgaccagcaa
    15961 cttgatccag tcgagcagct cggaggtcga gggcttcttc ttcagtcccg acacctcgcg
    16021 caggcggaaa aacgacgcga gcgcctcggc gagcagcgac tgcttgaggc ccgggaaatg
    16081 cacgtcgacg atgcggcgca tcgtcacctc gtcggggaag cggatgtaat ggaagaagca
    16141 gcggcgcaga aaggcgtcgg gcagctcttt ctcgttattg ctcgtgatca ggatgatcgg
    16201 ccggtgcttc gccttgacga gctggcgcgt ctcgtagacg tagaactcca tgcggtcgag
    16261 ttcacgcagc aggtcgttgg ggaattcgat gtcggccttg tcgatctcgt cgatcagcag
    16321 caccggctgc gtgttggctt cgaaggcctc ccacagcttg cccttgacga tgtagttggc
    16381 gatatcgtgc acgcgtccat cgccgagctg cgagtcgcgc agacgcgaga ccgcgtcata
    16441 ttcgtacaga ccctgctgcg ccttgctggt cgacttgata tgccactcgt acagtggccg
    16501 gccgagcgag gccgcgactt cttcggcgag ctgtgtcttg ccggtaccgg gctcgccctt
    16561 gatgagcagc ggacgcgaca gcgtgacggc ggcgtttacc gccatggcga gctccggagt
    16621 cgcgatgtag cgctccgagc cgctgaatcg ggaattggtc atttgagcac cagcttatgc
    16681 tgcgcgggcc ccggcaggaa gggtgtcggc gcaccacgcg cccagtcatc gaaaccggcg
    16741 cgaaaaaacg gcgacagcgg atggccgctc gcgccgcccg cgacggtgag gtaggcatcc
    16801 gcctcgtggc cgggggagac cgcaaagcgc tcggatgcgc cgaatgccgc gccctgcacc
    16861 cgcggcatgt cgtgatcgcc cggcagctcg ggcacgtgca ttgcgagcag ccgtcgcccg
    16921 agcagcggca gtgcgcccgc gagcggatgc gcgatgccca ccgcgcgctg gcggccccag
    16981 gcgcaacgcg caagggcgcc gcattgccgc tcgagttccg tcgccgcggc atcgacctgt
    17041 gcgagcagga acgcgcgcca gtcgacatat gccggcgcca gccagtgcgc cgggcgcgcc
    17101 gtggcgagct gccaggccgt cgcggcgaac tgcgccggcg gcggatatgc ctcgccggcg
    17161 aggcccgcgc cggcgagcac catggaccag gtcgcgtcca cgacctgctg ctgcgtcctg
    17221 cgcaccagcg tatagccgac ggcgtccacg gcggccgcgt ccatcggggc cgatatcagt
    17281 tcgcgaaagg cggcgcgccg cggcgcgccg tcgagcgcgg ccgcatcgag cagctcgagc
    17341 aacagctgcc gccacggcgc gagatagagc gcccgatcgt cgagctggat cgcgagcatg
    17401 tcggcgggcg ttgccggcgc ccgcagcgct gcgagaccgt cgcgaatctg catcgcgcgc
    17461 gcgccgaggt cgaagcccac accgtgcgca tcttcgtcgt cgccgatcag tgcgccggct
    17521 tcgccgccga cgacgcgcgc attcgcgctc cacaggctgc cggtggcggg atcgacaacg
    17581 cggggctgca tcgacggcgc gagataggcc ccaccggccg ctcgcagcgg accgcgcgtg
    17641 gcgggaatgc gcccgagcag cgtccaggcg atgtgtccgt cacggtcacc gaccatcatg
    17701 ttctggtgcg gaatgccgac gccggccgcg acttcgagcg cgtccgctac cgaagcggcg
    17761 ctctcgagcc ccagcagtcc gagggtcgtc gcgcccggct gggtggcgac ccaggtcgcg
    17821 agctggcatt cgccctggcc gtcctgctcg cgcgtcgcga acacgacgcc ctccgcgccg
    17881 ctcctgatgc gcaggcgctc ggtctgcccg ccgcgcacgc gaatgcgcgc cgtgtcgatg
    17941 tcgaagggct gctcgacgcc cgcgagatcg cgccaggtgc cggcttcgag gtcgcactgc
    18001 gtccagcgca gatcgagcca gtcgccgtag ctgttggtga atccccaggc gatgcgcccg
    18061 ttcgatccgg cgacgagcgc cggcacgccg gcaagcgtga cgccgttcag atcgtagcgg
    18121 ccatcgtgcg tcgtgaggcg ggcgcgatac cacaccggtg gcacgtcgag tccgagatgc
    18181 atgtcgttgg cgacgagcgc ggcgcccgat gcggtacgcg cgccggcgag cgcccagttg
    18241 ttgctgccga tgccgcgcga cgcgccggtg cgacgcgcag ccgccgcctc ccgcggcgac
    18301 ggtgcatcgg tcgcggctcc cgtgcgcagg tcccatacct cggggccggg gattgcaggc
    18361 tcggcgagcg gcggcgactg cgactcgacg ggcgcatccc atgcgctgcc cgccggcagc
    18421 acgaacccga gcaccgcggc tgcacgcgcg cctccgaggc gctgcgtgag cgcctcggtc
    18481 agcgccagac ggcgctgctc accggcgaaa tcgtggtact gcagctgcca ccacatggag
    18541 tggacgacga gcgccgtgtc ctcggcgcgc cacacccgcg gcacggcgcg caacaaccag
    18601 tattcccacg ggcgactgcg cagactcgcg aggcccgcgt tcacgccgtc cacgtaggcg
    18661 tcgacgaccg cgcgctcctc gcgcgtcagg ctctcggcca gcgcacgcgc aacgcgccgg
    18721 aagcggaaca ggcgcgcctt gcgatcctgc tcgagcgcca cctcgccgaa cagttccgca
    18781 agctcgccgg cggcgaggcg ccgagcgagg tccatctgga agtagcggtc ctgcccgtgc
    18841 gtaaagccga gcccataggc gagcgccgca cggtccgagc cgctcaccgt gggcgtgccg
    18901 agcgcatcgc gctcgatcgt caccggcgcg gccagccgcg cgacctcgat ctcgccgtcg
    18961 agctgcggca acgatgcgcg catcagcagc cacgcgccga tcaccgccgc gattgcggcg
    19021 gcgagcagga catacgcgag gcggcggagg aagcgcaaca tctactcgcg cgtgacctcg
    19081 agaatctgca tcgcattggt gccgcccgat acgcccgtga gcgcgccggt cgtgatgatc
    19141 acgcgatcgc cggaatcgac gaggcgcagt tccagcaacc gcgcgaagat cgactcctgc
    19201 acgtgcgcgc catcggcttc ggtcacgtcg aagggtaccg ggtacacgcc gcggtagagg
    19261 gtcacccgcc ggctggtcgc gggatggcgc gtgaacgcgt agatcgggat gtccgagcgg
    19321 atgcgcgaca tccacagcgg cgtcgaaccg gattcagtca gtgcgacgat ggcgcgtacg
    19381 cgcatgtggt tcgccgtgta catgaccgcc atcgcgatcg cctcgtcggt gcgggtgaaa
    19441 tgcccgacgt tgcggtcgcg cgcgcgcgcg atcgacagct ggtatttctc cgcgccctcg
    19501 ataacctggg ccatggcctc cacggccttg acgggaaagc ggcccacggc cgtctccgcc
    19561 gacagcatca ccgcatcggt gccgtccatc accgcgttgg cgacatcgct cacctcggcg
    19621 cgggtcggca ccggactctg gatcatcgac tccatcatct gcgtggcggt gatcgcgacg
    19681 cggaacttgc cgcgcgcctg gtgcaggatc gtcttctgca gaccggtgat ctccgcgtag
    19741 cccatttcga cgccgaggtc gccgcgggcg accatcaccg catcggtctc gtcgagaatg
    19801 gacggcaggt tctccaccga ttcgtggcgc tcgatcttgg cgacgatgcg cccctgcccg
    19861 ccccactcgc gcagcttcgc gcgcgcctcg cggatgtcgg cggcatcgcg cgcaaaggac
    19921 acggcgacgt agtcgatctc ctggccggcc gcgaaacgga tgtcgtcgcg atccttgtcg
    19981 gacaaggccg gcgccgagat gcctccgccc ttgcggttca gccccttcct gttcgagagt
    20041 tcgccaccga tgcgcacgac ggtttcgacg cgcgggccat cgacggccgt gacgtcgagc
    20101 acgatctggc cgtcggcgag cagtagcgtg tcgccggcgc tcacgtcgcg gtggaggttg
    20161 ccgtaggcga tgccgacctc atgctgcacg ccggcgtccg cggcgatgcg cgggtcgagc
    20221 gcgaatgcct ggccttcggc gagccgcacg cgtccgtcgc gaaagctctc gatgcggatc
    20281 tttggcccgc cgaggtcggc caggatgccg acgatcttgc cggcgcgtgc cgccgcctcg
    20341 cgcaccagcg cgacgcgacg cgcatggtcg gcgtgatcgc cgtgggaaaa attgatgcgg
    20401 gcgacatcga gccccgctcg caccatgtcg gtgagcacga gacagtcatc ggtcgcgggg
    20461 ccgagcgtgg ccacgatctt ggtacggcgc tgcatggctc gcaaattagc acgaccgcag
    20521 gccggtcgtt agcagtcgat cagcctgagc cgcgtgcctc gagtgcggcg acggccggca
    20581 acgtcttgcc ctcgacgaac tcgaggaacg ccccgccacc ggtcgagatg tacgagatgc
    20641 cctcggcgac accgtacttc tcgatcgcgg cgagcgtgtc accgccaccg gccagcgaga
    20701 acgccttgct gcgggcgatc gcattggcga ttgctttcgt gccctcgccg aactgcggga
    20761 actcgaacac gccgaccggc ccgttccaga tcaccgtgcc ggcggcctcg atgagcgctg
    20821 cgaaccgctc ggccgtatcc ggcccgatgt cgaggatcag ctcctcgggt ccgacatcgt
    20881 ggatcgagcg cacgtcggcg tgcgcggtcg cggcgaactc gcgcgctacg accacgtcgg
    20941 tcggcagcgg aatctcggtg ccgcgcgcgc gcgcctgctc gagcagcgag cgcgcgatat
    21001 cgagcatgtc cgcttcatgc agcgacttgc cgaccgcgac gccggtcgcg gcgaggaagg
    21061 tattggcaat gccgccgccg acaatcagct tgtcgacctt ggcgatcagc gactcgagca
    21121 ccgtcagttt ggtcgacacc ttcgcgccgg caacgatcgc gacgagcggg cgggcgggcg
    21181 cgtcgagcgc gcgctcgagc gcctcgagtt cggcgaccag cagcgggccc gcgcaggcta
    21241 ccggtgcgaa agttgcgacc ccgtgggtgc tcgcctcggc gcggtgcgcc gtgccgaacg
    21301 cgtccatcac gaacacgtcg cacagcgccg ccatgcgccg cgccagcgtc gcatcgttcg
    21361 ccttctcgcc cttgttgaag cgcacgttct cgcacagcac cgcttcgccg tagccgatgt
    21421 cgacaccgcc ggcgagccag ttctcgcgca gtggcaccgg cttgccgagc agctccgaga
    21481 ggcgcacggc caccggcgcg agcgagttct ccgcagagta gtggccttcg accggccgcc
    21541 cgaggtgaga gaggatgatc accttggcgc cctgctgcat cgcccggcgg atcgtcggca
    21601 gcgcggcgcg gatccgcgcg tcgctggtca ccacgccctt gtcgatcggc acgttcaggt
    21661 cctcgcggat catcacccgc ttgcccgcga ggtcgacatc actcatgcgc agcagcttca
    21721 cggcgcgtcc tacttggcgc gcgaccaggc ggacacgacg tcgagcaggc ggttggagaa
    21781 gccccactcg ttgtcgtacc aggaacagac cttgaccagc ttgccgtcga tcaccttggt
    21841 catcgtcgtg tcgtaggtcg aggaatgcgg atcatggttg tagtcgatcg agaccagcgg
    21901 ctcgctggtg tatgcaagca cgcccttcag cggaccctgt gacgcggcca gcatcagctt
    21961 gtcgatttcc tcgaccgtgg tcgcgcgctg gggcgtgaac gtcaggtcga cgagcgacac
    22021 gttgatcgtc ggcacgcgca cggcgtaacc gtcgagcttg cccttcagcg ccggcagcac
    22081 cagcccgagt gccgcggccg ccccggtctt ggtcgggatc atcgacatcg tcgccgagcg
    22141 cgcgcgccgc aggtcggagt gatagacgtc ggtgagcacc tggtcgttgg tgtaggagtg
    22201 gatggtcgtc atcagcccgc cgacgatgcc gatgctctcg tgcagcacct tggcgaccgg
    22261 cgcgaggcag ttggtcgtgc acgaggcgtt cgagacgact tcgtggctcg ccttcaggtc
    22321 accgtggttg acgccataga cgaccgtggc atcgacgtcc gagccgccgg gcgccgagat
    22381 cagcaccttc ttcgcgccgc ccttgaggtg cgcgcccgcc ttcgccttgc tggtgaagtg
    22441 gccggtgctc tcgatgacga tatcgacccc gagttcgccc cagggcagct tcgccgggtc
    22501 gcgctcggcg aatacgcgca cgcgatcgcc gttgatcagc atcgaatcgc cgtcgacgcg
    22561 cacttcgccg gggaacttgc cgtgggcggt gtcgtggcgg gtcaggtggg cattggtgtt
    22621 cgcgtcgccg aggtcgttga ccgcgacgat ctggatttcg tgcgtgcgct tcgtttcata
    22681 cagtgcccgc agcgtgttgc ggccgatgcg cccgtacccg ttgatgccta ccttgattgc
    22741 catgaccgtt ccttcctccg taccacttac gcgagcaatt tacgcgagca atttgccgac
    22801 ggccgccacg atgtggtcgg ccgtgatgcc gaaatgcctg gacaggtccg ccgccttgcc
    22861 cgaggcgccg aagctgtcga taccgatcac ctcgccgtcg ctgccgacgt agcgccacca
    22921 gctctggcgg gcggccgcct ctacggcgag acggcgcgtg accgcccgcg gcagcaccgc
    22981 ctcgcgccac gcggcatcct gcgcatcgaa ggcgccgctc gacggcatcg acacgagccg
    23041 cacgcgccgg ccctgcgcct ggagcgcgcg cacggcgtcc gccgcgatgc cgacctcaga
    23101 accgcacgcg atcacgatcg cctcgggaac gccctccgtg tcgatcagcg tatagccacc
    23161 gcgcgcgacc gcggccagtt gttccgcggt acgcggctgc tgcggcagcg cctgccgggt
    23221 cagcacgagg gccgtcggcc cgttcatgcg ctcgagcgcc atgcgccacg cgatcgccgt
    23281 ctcgacggcg tccgcagggc gccacagcga cagacccggc atcgcgcgca ggctcgcgag
    23341 gtgttcgacc ggctggtggg tcgggccatc ctcgccgagg ccgatcgaat cgtgcgtgta
    23401 cacgaggatc accggctggt gcatcatcgc cgcgaggcgc acggcattgc gcgcatagtc
    23461 ggagaagacg aggaacgtgc ccgcgtacgg ccggaaaccg ccgtgcagcg tgaagccgct
    23521 catcatcgcg gtcatcgcga actcgcgcac gccgtaatgg atgtagttgc cgcccgcgtt
    23581 gtcggcggtc acggtgcgcg aatccttgcg cagcgtattc accgagccgg tgaggtccgc
    23641 cgaaccgccg agcagttcgg gcaggcccgg cccgatgaca tcgagcacgg cacccgacga
    23701 cacgcgcgtc gcctgaggcg actcgagcgc gcgcgcggcg gcgagcgccg catcgagcag
    23761 cgcggcgtgg ttgtccggca atcgccccgc catgcgccgc tcgtactcgg ccgcgagttc
    23821 ggggaacgcc ttgcggtagc ggcggaagcg gcgctgccag ctcttctcgg ccgcctcacc
    23881 ctgcgcacgg cggtcccact gcgcacggat ctcatccggg atctcgaacg gcccgtgggg
    23941 ccagccgagc gtggtgcgcg ccgccgagac ttcctcgacg ccgagcgcct cgccatgcac
    24001 gctcttggtg ccctgcttgt tcggagcacc gaagccgatg atcgtgcgcg cgagaatcat
    24061 ggtgggctgg ttcttgtggt tgcgcgcctt gcgcagcgcc gacgcgacgg ccgccgaatc
    24121 gtggccatcc acgtccttga tgacgtgcca accataggcg gcaaagcgcc gggcgacatc
    24181 gtcggtgcac cagccctgca ccttgccatc gatcgagata ccgttgtcgt cgaacaccgc
    24241 gatcagcttg ccgagcttca gcgtaccggc gagcgaggcc gcctcgtgcg agatgccctc
    24301 catcaggcag ccgtcgccga cgaacacata ggtgtggtgg ccgacgatct cgtggcccgg
    24361 gcgattgaac tcggccgcca gcatcttctc ggcgagcgcc atgccgacgg cgttggcaaa
    24421 gccctggccg agcgggccgg tggtcgtctc gatgccgatg gcaaggtcgc gctccgggtg
    24481 cccggcggtg cgcgcaccaa gctggcggaa attgcggatt tcctcgagcg tgagcggata
    24541 gccggtcagg tgcagcaccg cgtacagcag catcgaggcg tggccgttcg acagcacgaa
    24601 gcggtcgcgg tcgacccagc gcgggttgcg cgggttgtac ttcagcacat cgcgccacag
    24661 cacctcggcg atgtccgcca tgcccatcgg cgcacccgga tgtcccgact tcgcggcctg
    24721 gacggcgtcc atggcaaggg cacggatggc attggcgagg tcgcggcgtg tcggcatggg
    24781 tgggcagata ccgggcagaa aaagcgggcg cgcattgtcc atcaccggcg ccgcgacagc
    24841 aaaccggggc aaagtctgca tggggttgcc gactgccact gcgcggtcgc gccagctata
    24901 atgggagttt aattgcctcg accccaacac tgatgcccaa cagctacctg ttcacctccg
    24961 agtcggtttc ggaaggtcac ccggacaaga tcgccgatca gatctccgat gcggtgctcg
    25021 acgagatcct gacccaggat ccccgtgggc gcgtggcctg cgagacgctg atcaagaccg
    25081 gcgtggtgat cgtcgccggt gaggtcacca ccagcgcgtg ggtggatctc gaggcgctgg
    25141 tgcgcaggac cgtgctcggc atcggctaca accattccga catgggcttc gacggcgcca
    25201 cctgcggcgt gctcaacgtg atcggcaagc agtcggccga catcgcccag ggcgtcgacc
    25261 gcaaggacga gcgcaagcag ggcgccggcg accagggcct gatgttcggt tatgcgagca
    25321 acgagaccga cgtgctgatg cccgccccga tcacctacgc gcatcggctg gtgcagcgtc
    25381 aggcgcagat gcgcaagcgc ggcaagctcc gctggctgcg cccggacgcc aagagccagg
    25441 tgtcgctgcg ctatgaggac aacaagccgg tcgcgatcga cgcggtcgtg ctctccaccc
    25501 agcactcgcc cgacgtgtcg acgaagacgc tgcgcgaggc ggtgatggac gaggtgatcc
    25561 tgccggtgct gccgaagaag tggctgcaca agggcacgaa gttccacatc aacccgaccg
    25621 ggcgcttcgt gatcggcgga ccggtcggcg attgcggcct gaccgggcgc aagatcatcg
    25681 tcgataccta tggcggcttc gcgcgccacg gcggtggggc gttctcgggc aaggatccgt
    25741 cgaaggtcga ccgctcggcc gcgtacgcgt gccgctacgt ggcgaagaac atcgtcgccg
    25801 ccggcctcgc cgaccgctgc gaggtacagg tgagctacgc gatcggcgtc gccgagccga
    25861 cctcgatcat ggtcgagacc ttcggcaccg gaaagctgcc gcgcgagaag ctgacccagc
    25921 tcgtgcgccg cttcttcgat ctgacgccct acggcctgcg cgagatgctc gacctgaccc
    25981 gcccgatcta tttgaagacc gcggcgtacg gccacttcgg ccgcgaggac gccgatttca
    26041 cctgggagcg caccgacaag gcggcagcgc tcgcagcgga gctgcggcgc acgcgcgcac
    26101 gggccgcgta acggcaacga gtttgtggaa taccggctgc cagggtggcg gccgggcgtc
    26161 gacctgagga gcgctgcgac aggggcaagc cctgccaggc tcaggaaccg acgtgccagg
    26221 tatcaacggc gctcgtcatg atttcggagg acgacatgac tgcagtagcc aagccgtcgg
    26281 gcacgacgcc cgactatttc gtacgcgaca tcagggaagc ggactggggc cgcaaggaga
    26341 tggcaatcgc cgagaccgaa atgcccggtc tgatggcgat ccgcaaggag ttcggcccgg
    26401 tacttccgct gcgcggcgcg cgcatcgccg gatcgctcca catgacgatc cagaccgcca
    26461 tgctgatcga tacgctgaag cacctcggcg ccgacgtgcg ttgggcgtcg tgcaacatct
    26521 actcgacgca ggaccatgcc gccgcggcga tcgccgcggg cggcacgccg gtgttcgcct
    26581 acaagggcga gaccctcgac gagtactggc agttcaccca cgccatcttc gactggccgg
    26641 gcaaccagta cgccaacatg atcctcgacg acggcggcga cgccacgctg ctgctgctgc
    26701 tcggcgcgca ggccgagaag gacccgtcgg tgatcgccaa gccgaccaac gaggaagagg
    26761 tcgcgctgtt cgccgccatc cgggcacggc tcggccagag ccctgactgg tattcgaagc
    26821 ggctggcgca catcaagggg gtcaccgaag agaccaccac cggcgtcaag cggctctacc
    26881 agttgcaacg cgacggcaag ctgcccttcc ccgcgatcaa cgtcaacgat tcggtcacca
    26941 agtcgaagtt cgacaacctg tacggctgcc gcgagtcgct ggtcgacgcg atcaagcgcg
    27001 ccaccgacgt gatggtcgcc ggcaaggtgg ccgtggtcgc cggctacggc gacgtaggca
    27061 agggttcggc cgaatcgctg cgcgccctct cggcgcaggt ctggatcacg gagatcgacc
    27121 cgatctgcgc gctgcaggcc gcgatggagg gcttcaaggt cgtcaccatg gacgaggcct
    27181 gcgacaaggc cgacatcttc gtcaccgcga ccggcaacct caacgtgatc acgcacgagc
    27241 acatgcgccg catgaagcac caggcgatcg tgtgcaacat cggccacttc gacagcgaga
    27301 tcgagatcgc ggccctcaag cagtacaagt gggaacaggt gaagccgcag gtggaccacg
    27361 tgatcttccc tgacggcaag cgcctgatcg tgctggccga gggccggctc gtgaacctgg
    27421 gttgcggcac cgggcatccg tccttcgtga tgtccaattc gttcaccaac caggtgctgg
    27481 cgcagatcga gctgttcgcg cacggtgaca agtacggcaa ggaggtctac gtgctgccga
    27541 agcatctcga cgagaaggtc gcgcagctgc acctcgagcg tatcggcgcg aagctgacga
    27601 cactgacgcc ggagcaggcg gcatacatcg gcgtggcgcc acagggcccg ttcaagcccg
    27661 agcactaccg ctactagccg cgcgcccggg ccctctggtc gggcccgggg ccccgaccac
    27721 ggtacacagg cccggaagcg tgctcgcgct tccgggcctg cctttgtgtg atgcacttgg
    27781 catcacgaca ttaccggtgc gccacggcac ataagccggc cggattccgt gaaatgtgtc
    27841 gcgtcacgtc gccggtcggc gacggaatat tcctcttggt ccggccattg acggcggaca
    27901 cgggcaaggg ggtacgcgga tgcaagagtc aggatcgacc gcacaggcac aggcacacgc
    27961 accagccgaa atcccgggcg gtcattcccg caagacgcgc aaggacgtca tcgtcttcac
    28021 gtccgatgac gaattcctgc tgagcctcgg gccggcgatg gacgatcgct ttcgttcaca
    28081 caccaccgac ggcaccggcg cgtgggccga aatggttcgc ggccgtgcgg gcgtcgcgct
    28141 gatcgacgcc gccacgctcg ccaatgcgcc cgagatcgtg cgcactatgg aggcggagtt
    28201 cccctcgttc gccctggtgg tggtggcgcc cgcggcggcg cgcccgcaat ggaccctcgc
    28261 gctgacgcgc ggcgcgatca tgcagctgct cgaacgcgag aacctggaaa cgaacgccat
    28321 cgccgcggcc ctcactgccg agccgcgcgt tgcctccgca ggcgccgcga cggcatccgg
    28381 cgccgccggc gacggatcca cgccgcgcaa gctgcccctg ccgatgatcg cggcggccgc
    28441 ggccgccgtg ctcgtgctcg ccggtgccgc gtggctgtgg ttgggcggcg atgactcctc
    28501 acccgcgacg atcgcggacg aacctgccgc aacgactgca acgcccgtgg ccgaaccggc
    28561 cgaggcgccc gcacaggccg acgcggcgcc ggccgacacg cgctcggttc ccgaactgct
    28621 gtccgccgcc cgcattgcct tcgccgagcg gcgctatgtc gagccggcca cgggcaacgc
    28681 gctcgcgctc tactctcgcg tgctcgccgt cgagccggcc aatgccgagg cgatcgatgg
    28741 cgtgcagcgc atcgtcgcga tcgtcgccgg ccaggcgcag gcgcagatca aagccgggca
    28801 gatcgacgag gcggcgaagc tgctcgacgt gctgcgcgtc gccgcgccca acgactcggc
    28861 gctcgccgcg gtcgaggcgg acatggccgc cgcgcgcccg aaatggctag cagctcgcgc
    28921 ccgcgaggcg atctcgaacg accagttcca gggcgcggag cggctgatcg acgaactcgc
    28981 cgcgctcggc accgaaaagg cgctggtgca ggacctcagg cgcagcatcg atgcacagcg
    29041 caggaacgcc gacctggcgc gcgcggttgc cgaggcgcgc gcgtcgctga ctccggcgct
    29101 gctgctcgac acctccgccc gcggaccgtc ggcacagctc gcgtcgctgc agcagatcga
    29161 ccggcgccat tcccaggtgg tcgccttcca gcgcgactac ctggccgcgc tgacccgcgc
    29221 ggcccgtgac gcgaccgggt caggtgattt cgccggcgcc gaacgctatc tcgcggccgc
    29281 taccaaggcc ggcggctcgc gcgacgccac cgacgcgcgc aaggagctgc aggccgcgcg
    29341 cgacgccgcc gccgcacgcg agcagcagcg cgccgaaacc gcgcagcgta cggcgcgcag
    29401 ccctgcgccg gcagcgggcg cggcggctgc gggcacggcg gccacggcac cgccgcccag
    29461 gatgccgaag gcgaagaagc gcggcgcgcc gaagtacccg gcgcaggccg aacgcgacgg
    29521 catcgagggt gaggtggtgg tcggtttcgc gctgactccc gagggtcgcc cgcgcgacct
    29581 tcaggtcatc gaatcgacgc cgcccggcgt gttcgacgcc gccgccatgg atgcggtgcg
    29641 cggctggcgt tacgagcaga tcagtgcggc cgacgcagag cggctgccgc gcgcctcggt
    29701 gcgcttgacc ttcaggatcg gagaccgatg atgaaacgcc ggcgcaccga cttcgacctc
    29761 gcgcagctga tgccggtcgt cgtcgccacg accttcgcag cggtgctgac gctgctgctg
    29821 accctcggca tccagcgggc ctcgcagctg cagtcggcat cgagcgcgct gcagctcgcc
    29881 tcggagctgc gctcgctgcc gcagtacttc ggcaccgagt tgacgctggt gcagcgcgga
    29941 ctcgagtcgc gcacgttcat cggcgagccg ctggcggaca tcgccgccgc gcgcgagggc
    30001 ttcgacaagg cgctcgagcg catcgacgcc gacctgatcg cggccggcct cggcggcacc
    30061 gtcgagatcc agcgcgtgcg cggcgcgctc gacgccgcct gggcacgctt gtcgccgctg
    30121 ctcgttgcgg tcgacagcca caagggcacg ccctactccg acacgctctc cggttcgcgt
    30181 ctgaatgcca ccggtcgcgc gctgaaggcc gacgtggatg ccgcgctcgc ggcgcaggac
    30241 gattcgatgc gcgacgtcgg cggcgcgctc aaggacctcg cgaccggcct gcaggacgcg
    30301 gtcggcgacc ggggcggcag cctgcgcagc ttcctgctga tcggcgctgc cgtgtccacg
    30361 ctgctgctcg ccctgatgct ctacttcgcg ctgcgctcgc ggcgcgcgac cgacgacgcg
    30421 cgccgtgccg aacgccaggt cgcggacatc ctcggcaccg tgcgcgaggg cctgttcctg
    30481 ctcgaccgca acctggcgct cggcgccgtc tactccgcct ccctcacccg catcctgcac
    30541 accgagaacg tgcgcgccac ctcgttcgag gacctgctgc gcccgctggt gccggccaag
    30601 acgctcgcca cggcaatgaa gttcgtgaac ctgctgtgga aggagaaggt gcacgaggac
    30661 ctgatcgata cggtcaatcc gctgaaccag atcgaagtgc agttcccgaa gtccggcggc
    30721 ggcaccgatt cgcgcttcct gtcgttcgcg ttcaagcgcg tcaggaatgc cggcggccag
    30781 ggcgagcacc tgctcggcat cgtcaccgac gtcaccgatc gggtgcagat ctcgcgcgaa
    30841 ctcgagcagt cgaaggccga gaaccaggcg cagctcgacc tcgtcatgca gttgctgcag
    30901 gtgccgggcg cgcagctgca gtcgttcgtg cgcgacgccg acatggcgct gcgcaagtgc
    30961 aacgcgatcc tgaagcagcc cggccgcgaa gaggccgaac tgcgctcgaa gctgaatggc
    31021 gtgttccgcg aagtgcacac gatcaagggc gaggccggcg cgctgtcgct gacctccgtc
    31081 gcctcgcgcg tgcaccagct cgaggaaagc ctggtggcgc tccgcgaccg cgcgcagatc
    31141 gacggcaacg acttcatccc gatcgtcgtc aagctcgacg aactgatgtc ctacctcggc
    31201 ggcgtcggcc agttcgccga ccgactgagc gaagtgcaac ccaagggcga tacgatgaag
    31261 tccgtcgtgc cgccgctgct cgcgaaccag ctgctcacgt ccgaggagcc cacgctcgaa
    31321 caggtgctgg ccaaggtcgt caaggacacc gcgcaggcgc acggcaagcg cgtggcgctg
    31381 cagtgccgcg gcctcgaggc gatcccgccg gcctatcgca aggcggcgcg ggacatcacg
    31441 atccacatgg cgcgcaacgc cgtcgtgcac ggcatcgaga caccggacca ccgcgtctcg
    31501 gcgagcaaga gcgagaccgc cgcagtgcgc gtcagcttcg atgaggcggg cgccgacggt
    31561 tacgtgctga ccgtcgaaga cgacggtcag ggcctgccgt acgagcgcat cctcgacatg
    31621 gcgctgcgca aggggctcgt gtcgccggcc gatgcggcgc gcatggagcg ccccgccgtg
    31681 tacgggctga tattccgcgc cggcttctcg acggccgaca aggtcaccga gcacgccggc
    31741 cgcggcgtgg gtctcgacgc ggtgagccag acggtgcgcg acctcggcgg ctcgatcggc
    31801 atcgcgacgg cggatggcaa gttcacacgc ttccgcatga agctgccgca ggcggccgtg
    31861 caggccggcg cgagccacgt cgcctgagtc cgcagcccat gaacgccgta cgccagcacc
    31921 gcctgatgat cgtcgacgac tcgaatatca tgcgtcgcaa gatcgagcgc tcgcaggaca
    31981 tcccgcaggt ggagatcgtc ggcctcgcgg gcaatggccg cgaggcactc gagctgcacg
    32041 agcagactcg cccgacgctc gtcaccatgg acctgacgat gccggagatg gacggcatcg
    32101 aatgcgtcga gcagctggtc ggccgcgacc ccgacatccg catcctcgtg atctccgcgc
    32161 tggccgacaa ggccacggcg atcgaggcgc tcgagaaggg cgccagcggc tttctctgca
    32221 aaccgttcac cgaacgccag ctcaacgatg cgctggtgaa gctgatcgag agctgagcgg
    32281 gcaccatgtc actgcaggga gaaatcggca cctttgtcgc ggcggtgctc aactatttcg
    32341 acaccgccgt gcagcagccg gccgtggtcg gcacgcccta cctcgcgctc ggcaaggcgc
    32401 ccgaggtctt cgactacacc ggcatgatcc gcatctccgg caagcgcaag ggcgtggtct
    32461 atttcaccgc gccgaagggc atgctctccg tgatgctgat gcgcatgcag gaaaccgaca
    32521 tgagcgacgc gaacctgcgc gacctcgtcg gcgaggtggc gaacacgctc tccggcaatg
    32581 cgcgcaggga cttcgggcgt aatttcatca tctcgactcc gtcggtcgtg gccggcagct
    32641 cgaccacgat cggctattca cccgacacgc gcccgatcgt cgtgccgatc gactggcgtg
    32701 cctaccacgc caatctcgtc gtctgcctca actagccggc gcgcctagcc gcgcttgcgc
    32761 gcgagcagca ggccgtcgcc gatcggcacc agcgacatgt cgacccgctc gtcgtcgcgt
    32821 gcgcgatcgt tgagcgcgcg gatcgcgttg gtctccgcgt cctgcacgcc ggcatcggcc
    32881 acggcaccgg cccacagcac gttgtcgacc accagcaggc cacccgggcg cagcagctcg
    32941 acgacgcgct cgtagtactc caggtagttt tccttgtcgg cgtcgaggaa ggcgaagtcg
    33001 aacgaggagc cgccgccctg cagcagctca tcgagggtgg tgagcgccgg cgcaatgcgc
    33061 aggtcgatgc gcgcatcgac gcccgcctcg cgccagaagc ggcgcgcgat ttgcgtccac
    33121 tcgaggctga cgtcgcaggc gatgagttcg ccgtcgcggg gcatcgcgag cgcgacacag
    33181 agtgccgaat atccggtgaa ggtgccgatc tcgaggcagc ggcgcgcgcc gatcaggcgc
    33241 accagcagct ggatcagctg gccctgctcc ggcgcgatct gcatgttggc ctgcggcatc
    33301 ttcgcggtga gctggcgtag cgccgcgagg atcgccggct cgcgcaccgt gtgctcgatc
    33361 aggtagcgct gca
//
LOCUS       DAOQVY010000011        32291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_1610_length_32291_cov_5.925115, whole genome shotgun sequence.
ACCESSION   DAOQVY010000011 DAOQVY010000000
VERSION     DAOQVY010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 32291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32291
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1610_length_32291_cov_5.925115"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PKN91_02110"
     CDS             <1..341
                     /locus_tag="PKN91_02110"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNS26893.1"
                     /translation="KRWFHCSRCRRARSAAHATNAFYRRDQLTWVRGEDRVERYSLAG
                     AQRFGQDFCRRCGSPVARVVASTGYVVVPCGSLDDAPGVEPRGHIFVGSKAPWHEITD
                     DLPQWEALPA"
     gene            427..1851
                     /locus_tag="PKN91_02115"
     CDS             427..1851
                     /locus_tag="PKN91_02115"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNS26894.1"
                     /translation="MKFPLARCALIAALAIATWPAPLLAQIAPYQVKAREVYARIIGF
                     RTAAGQNQTGAMAEWLADVLRAGGVPASDIATLEAGGATAMLVRIPGRKAPGRTAARP
                     ILFSAHMDVVDARPADWQRDPFTLIEEDGYFYGRGTMDNKAGVTALVSTILRFTAERL
                     HPTRTLVFAFVGDEETGMETTRAIAAHPWVKDAEFAINADAGGGALDDATGKPLIYLV
                     QGAEKTYATFDLTVTNPGGHSSRPRADNAIYQLAHALDRIEAHRFPVMANELTRAYLG
                     AMGRAQTGAVGDALRRFAANPGDAAAADALAQSPEFVGTTRTTCVATMLQAGHAENAL
                     PQKATAAINCRIFPGVAVDTVRDELVRTIDDPEVRVVVRGTPTASPVSEMRPDVTAAI
                     TRAVHAVHAGVAIVPYLESGGTDGKVYRTAGIPTFASSGLFSKPSEMFAHGLDERLPV
                     ASFYQGLDHIYRLAVDLGGVRGRR"
     gene            complement(1882..3096)
                     /gene="tal"
                     /locus_tag="PKN91_02120"
     CDS             complement(1882..3096)
                     /gene="tal"
                     /locus_tag="PKN91_02120"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNS26895.1"
                     /translation="MALGPLRRRACAHFHGSRQHLAMATTNPLLSLKDLCQSVWIDDL
                     RRAWLTDGTLSRLIALDGVTGVTSNPAILAKAITETDDYAAAIAGLAGSRLTARDLYE
                     TLVIADIQAAADLLLDIHRTTARTDGYVSLEVSPRLAHDCDATVAEGRRLWEAVARPN
                     LMIKVPATEAGLPAVRKLIAAGVNVNVTLLFSVSRYTAVAEAWIAGLEDRIQSGGDPG
                     SLPVSAASFFLSRIDSFVDRQLDAIGSPDAQALRGQAAIASARLAYEQYERLIRTPRW
                     RSLAAAGALPQRLLWASTSTKDDAYRDVRYVEALIAPETINTMPTTTLAAFRDHGQAA
                     VRIAEDLDTARALPGLLAALGIELDDVARRLEAEGVQKFLEPYEQLLAALGRGIAASA
                     DGSSNAARRSAR"
     gene            complement(3033..5492)
                     /locus_tag="PKN91_02125"
     CDS             complement(3033..5492)
                     /locus_tag="PKN91_02125"
                     /inference="COORDINATES: protein motif:HMM:NF003621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HNS26896.1"
                     /translation="MSNRHQRTARRGAGPSDTIDAPPLSAAELQAIDAHWRASNYLAV
                     GQIYLYDNPLLREPLRREHIKPRLLGHFGTTPGLNFIYTHLNRAIRKFDLDMIYIAGP
                     GHGGPAVVAQAWLDGTYSELYPDVSLDEAGLKRLFTQFSFPGGIPSHAAPETPGSIHE
                     GGELGYSLAHAYGAAFDNPDLIVACVVGDGEAETGPLATGWHGNKFLNPVRDGCVLPI
                     LHLNGYKIASPCILARIPAAELTQLLEGYGYHPHFVEGDDPGRMHQAMAATLDAVIGE
                     IRAIQHDARTRGFGGRPAWPMIVLRSPKGWTGPKEIDGKRTEGSWRSHQVPMGDMDKP
                     EHVAVLEQWLRSYRPDELFDERGAPVAEIAALAPRGERRMSANPHANGGVLLRDLELP
                     AIQDYAVAVVSPGEVSAESTRVQGRYLRDVMQRNLPHANFRVFSPDENNSNRWQDILE
                     VTDRTWVAEIHAGDDRLSPDGRVMEVLSEHQCQGWLEGYLLTGRHGFFSCYEAFIHIV
                     DSMFNQHAKWLKVCNHIPWRRPIASLNYLLSSHVWRQDHNGFSHQDPGFIDHVVNKKA
                     EVIRVYLPPDANTLLAVTDQCLRSRNCVNVVVAGKQPAPQWLPMDEAIRHCTAGVGIW
                     PWASNDEGADPDVVMACCGDVPTLETLAAVELLHQHLPELRVRVVNVVDLMKLQPAEE
                     HPHGLTSDEFDVLFTRDRPIIFAFHGYPSLIHQLTYRRTNHGNLHVRGYKEEGTTTTP
                     FDMVVLNDLDRFRLVQDVIRRVPGLGSRAAYVKQAMRDCRIEHKRYIHRYGEDMPAVA
                     GWRWGRSADVRARTSTEADNI"
     gene            complement(5897..6271)
                     /locus_tag="PKN91_02130"
     CDS             complement(5897..6271)
                     /locus_tag="PKN91_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26897.1"
                     /translation="MKKAALVLGILGGVIAGGIGMKWLSDIGALTEQQKMTAQLMGQG
                     DQLQAMGIAGLLLLGSLVAGIVGGIIAFRGRLMAGAVVMLAGGIVPLFFAKQAILFTA
                     VLIAGGVVAFMAHRKSVRPVVA"
     gene            complement(6387..8426)
                     /locus_tag="PKN91_02135"
     CDS             complement(6387..8426)
                     /locus_tag="PKN91_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS26898.1"
                     /translation="MTGTEGTGRKRGVRASRVRLAHALTEAGLRTQAALAERIADLEG
                     LDAAPKDLVSRLFRERPVEPQTLERVARALGVDAYSLYKTAREEARPAVASVHPQAEH
                     PQPAHLPPPTHPSRRRLWTLSVAVLVVVTASTWWFTRGPRERDLSPTSTASRALLGLG
                     SESLVVLPIKGDTEGALTGMLRAELGNTFAVATATAEVLTSDLDPVSASTRLRTDAAL
                     DGEIVTVGRLSAVRLFLSADGVRQQVWSQSLPAVALSSLLHDIARGASLAIRRATGLP
                     VPSRARGHFPLAPVQDEYLRGEYYLDRPSNELNLRRARGYFEAALRRDPNYARAHAGL
                     CQTLLEGHWMDNEERALKDAAVACGRAVQLDPEDRVVAAAHAHFLSRTGRNEEAIALY
                     ERTASAHPLDAAVLTGLAAARLQAWRASGERALLESAKDTARRAGEVDPAVWKPLFAL
                     ATMEWFDGDVAAAIAVSEQALARDENEYVMANLGSFYLCDGAFEKARDIYLRARALNP
                     ASYVGDEFLGTVYYFLGDFERSAALRQRAIAAVSGGEPEIHEMWGNLADSYRQVGDRG
                     AAVAAYLRAAEIAERDHLRGTAPAADRAARAYYYTMLDQLDPRTVPPKVLLGIADEID
                     SIGAELASAIDMRRLAQIYLQRGDLDMARASLRRATETCRGYARLPDLVALTDRG"
     gene            8647..9030
                     /locus_tag="PKN91_02140"
     CDS             8647..9030
                     /locus_tag="PKN91_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26899.1"
                     /translation="MNSTTAGVGAMTITLCIIAAGCGDTGTGTDSSPPFAGSFAPQFP
                     PGASRLDRVGVDTRWEFSPSGTVITRGPHRTVTWRYEIRGSEIRLTGVDDRNQGERRL
                     FTRADEHCIWDGHGSSVDVKFCPVE"
     gene            9092..9436
                     /locus_tag="PKN91_02145"
     CDS             9092..9436
                     /locus_tag="PKN91_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26900.1"
                     /translation="MAMMKLLIGATLFVVLAGCGGSAGTTDDRTAIIDACNTQQGAGW
                     LRKEYGENYCECYADTAQQKLDSDSYRTLAAASREELKAADDEAARETIARRNTGVYS
                     EASHHAQSCKRT"
     gene            9456..10085
                     /locus_tag="PKN91_02150"
     CDS             9456..10085
                     /locus_tag="PKN91_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26901.1"
                     /translation="MLLARQEEGRAMPIEKCHEGRKLLYKFAVSRLSGTVLGETTRSE
                     TTVSGKIYGGGTNSTYGTVAPVQGTIESETTRYQTVFLRDDDGSEHAIELVDLLVPCR
                     EGHKLTLWRLGKDLWVQARNQSTNQVYRYGKLKKLLYPKVAYFAIGILFGIYAVDLDK
                     SGLLGLLFAILVGLAIATIPCGLIAWMRERAIFEATGVQEQSGSRTPVR"
     gene            10249..10560
                     /locus_tag="PKN91_02155"
     CDS             10249..10560
                     /locus_tag="PKN91_02155"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HNS26902.1"
                     /translation="MPVYLVSVVELTNPGPNMKRYAERSAELIRQHGGRYLVRGKASQ
                     VVSGELFNNRVLIVTEFPALENYQAFRNSDAYQKECRPLREGTGIYDTGVFDAPPPGM
                     A"
     gene            complement(10752..12170)
                     /locus_tag="PKN91_02160"
     CDS             complement(10752..12170)
                     /locus_tag="PKN91_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26903.1"
                     /translation="MRHAKASLSVVILMLAGASPAGAVDPAALLRKCEQVKADRWDGV
                     THYVVVQSMMGQQVALPYERFEVVGPDGQRLPGFRPMTADSPYSSAELKMIAGKAKGL
                     GIGLSNEMQASGLPVGMLDHGGEPWASPDPSVMMGGAGTFLEAAAEAQDANQRERDAA
                     VAEAAADSAALGKVGRRLRHVGSESVDGVATEHLRAEGLGQRHATQGGGEMVIEKLDV
                     WMDAGNCVPIKFTMAGTHTSDGKTQPVTIERVNTQYRAVPGSKMYEPFRQVMRIQGAM
                     TEAQQKELRESQAKLEELDRQLAQMPPAQRDMVMQRMGPQMETMKRMASGGGIEVVTE
                     VQQILVNPDDEALRRVQLSAAASSMGGAGAMPTPMPTPMTMPASTAPAASAGQAGDQS
                     AYKACLEEKARQRQAAQKKKQGLGRLMGAVARNAGRLGGEAVTRAIGEARTAKATADD
                     LAAAARDLGLTEDEIAECQTAR"
     gene            complement(12256..14826)
                     /locus_tag="PKN91_02165"
     CDS             complement(12256..14826)
                     /locus_tag="PKN91_02165"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNS26904.1"
                     /translation="MAADSPTTGCGGALDRIYTGALERRGGERRRYLDEQCRADEPLR
                     REVEALLDAAEREGDPFAEQIQAAREHLFRSLLASDAKAEEDLSGQRFQNWRIERRLA
                     RGGLATVYLAQRDDGEFVQQAAFKVLRRGLDTEDVVARFRAERQILSSLEHPSIARFL
                     DGGALPDGRPYLVLEYVDGRPITDHCSERGLELRDRLRLLIRVLRALHHAHSHLVVHR
                     DVKPSNVLVSAEGEVKLLDFGIAKLLEPGPSPTGALHTRTGVALLTPGYGSPEQHARA
                     TVTTGSDIYQAGLLLYELLTGEFPFDGDPSAVAFQVPPPSQRLRGTALYRQVRGDLDA
                     ITLRAAHADPAQRYTSADEMRLDLEHFLAGRPVQAQPDRFSYRLRKLAGRNPWLMPLL
                     AVVVLGTIAYVVTLTVYSARLERERQLAAAAQQFMVELFRSPDPFAPADPARGRDIRV
                     VDTLELGQRRVREELGAQPELRAALLASISDVYASLDQHAEAIDLREEALALETELHG
                     PRSAQVASSLRALGYQYAATGDMERADVLLERQLEIARSSRGVSPADLAVARIASGLH
                     EERRGNLDRSHELLLQGVATLRAEPASHPNELVEALIVLEQHRSFADVPIDFDPLREA
                     EQVARRVFGADSLQAALVQVRLASSLTNRGEYDASEQHFLAAIPVLEARLGPNHGSTL
                     DALNNLGYLYHGQGDLAGAERLHGELLARKITKYGEQHSAVADSYQNFGSAITHQGRY
                     DESLPLHRKAYETFKVVLNEDNYVIAVPLLSIAYVQLRRGELAEAEAAAREALARLQA
                     TPAARHLQGVAQCLVGVSLERRGQAAEGGALIASSHALIGSASLPEPYPELCRVPVEG
                     "
     gene            complement(14816..15382)
                     /locus_tag="PKN91_02170"
     CDS             complement(14816..15382)
                     /locus_tag="PKN91_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HNS26905.1"
                     /translation="MTESLDIAALLEAVGRSEHGAMDELVRVIYPDLRRIAHFHLVKE
                     RPGHTLNTTAIVHEAYLRLAGGNANWNDREHFLRAASAVMRHLLVDYARQREAAKRGS
                     GLRPLTLTDAGPATDDDSVAVIALDDALRELSRIDQRLEQVMECRYFAGLSTRETAEA
                     LGMSERTVEREVLRAKGYLKRALQSGGG"
     gene            complement(15639..17645)
                     /locus_tag="PKN91_02175"
     CDS             complement(15639..17645)
                     /locus_tag="PKN91_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HNS26906.1"
                     /translation="MSAGQLAPAFTAATTAYTVTVEYSVASVTVSATAADAGARVNVN
                     GGGTGSAAVPLAVGNTSVTVQVTAADNATTRNYTITLTREPAALSNNADLASLVLSSG
                     NLDPAFDAAVADYAATVGFATASVTLTPVTADPGATVTVDGAAVVSGESSAPIALAEG
                     LNSFQVVVTAEDGQTTRSYSVAVTRLAAVDADVAQAAYLKAATTQDFFGRRLALDADT
                     LAVGVEWDDSAATGISPAHPDPFDNSAANAGAVYVFVRRSDGSWEPEAYLKASNAAAL
                     DFFGASLALDGDTLVVGAPGEDSASTGTAGDPADDAAIDAGAVYVFTRSAGTWTQQAY
                     LKASNTDAGDRFGQNVALHGDTLVVTATHEDSAAVGVDGDEADDSAEDAGAAYVFTRA
                     GDVWTQQAYLKASNAEAGDLFGRSVAVHGDSAAVSAEREASAATGIDGDQSDNSASGA
                     GAVYVFTRDGGGAWGQQAYIKASNTATGDQFGGGNLGSNGLALSGDILLVGARGEDSA
                     ATGIDGDQANNALRDSGAVYAFARTSGAWFQTAYIKASNPGIDDWFGASLDFDGEQMI
                     AAARNEGSAATGLDGDQADESAPGAGAAYLFAISAGQWTQQHYVKASNAESSDFFGSS
                     VGISQGRFAVGAEQEASTATGVDGDQSDNGAIGSGAVYLFEPVP"
     gene            complement(18002..18622)
                     /locus_tag="PKN91_02180"
     CDS             complement(18002..18622)
                     /locus_tag="PKN91_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNS26907.1"
                     /translation="MKNAHLAFVVALVAAASGPSVLNAEPVPQAERTPVDVRRTTLLV
                     RDIERSLPFYRDALGLKVVYDEQIGGGTGPDGRTQPPTIRLVLLRANDTFIGVLGLMQ
                     RLNVTHPPVKPLVKPQAGEVILVVNAKDLETRFARIRDTPGITVATAPERIEYPAPGG
                     QGTIPVLFSAVYDPDGYFIEVNQILGSAAGTVPPGTASAAQRSQQR"
     gene            18777..19772
                     /locus_tag="PKN91_02185"
     CDS             18777..19772
                     /locus_tag="PKN91_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26908.1"
                     /translation="MTEVVAPHGSPLGIAVLGVERFETSREFYCDLIGFDAGKLEAWA
                     GPDFESLWHLPAGSRARACLLTASGSAVGRILLVEFQAAHRKHVRGSMDSRAYGLVNL
                     NFYTRDIRAAARSFAALGYELWSEPTQYDLAAAVGKPIEVVFEGPDGVAVNLVELAST
                     DPGTRIGQMRAFVERHGYTRAGFTPVVTTSHVCRSIAKARAFYEQALRMGVLIDDEFN
                     SPAVNAFLRLKPGSRTHITFMQGNHMFGKIALSEPLNYACGDLTPLAVAPNIGYLAQA
                     FEVSDLARAIAGAAGTQAPIELEPRAMELPGLGRRSVAVVRNPGSGALQWLVESG"
     gene            19890..20201
                     /locus_tag="PKN91_02190"
     CDS             19890..20201
                     /locus_tag="PKN91_02190"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HNS26909.1"
                     /translation="MPVYLVSVVELTNPGPNMKRYAEKSAELIRQHGGRYLVRGKASE
                     VDCGDLFGNRVLIVIEFPAVENFQAFWNSDAYQKDCRPLREGTGIYDIGLFDAPPPGM
                     A"
     gene            complement(20251..21582)
                     /locus_tag="PKN91_02195"
     CDS             complement(20251..21582)
                     /locus_tag="PKN91_02195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS26910.1"
                     /translation="MTQNQPYPRPAYAWYCLGVICLAYLFGFMDRIIVGLLTPAIQAD
                     LGLTDSQMGIIQGLAFALFYTVFGLPIGLAADRTNRKRLLTAGTAIWSLATAGAGLVR
                     SFWGLFFMRAGVGAGEATLNPCTTSLIGDYFEPKSRPKAFGIYTMSTALGTGVTYLGG
                     GLVLAFVGVGTGGNTFNMPVLGEVPAWQAVFIIIGMAGLVPALLMALTVREPARRDLA
                     AGTSQKASWADIRAFLRQNRTTLLCHHFGVAFIIMAVYGWVNWMPAFFVRIHEWPVAR
                     FSIWYGIFGGLTGVLSAISSGFVTNWLKAKGFTDGTMRTVLIGGLGLTIGTGVAPLMP
                     SPELALAGYCIAGIFANYPPAQALAAIAEMTPNQLRGIVTAIYVLVIGIGGAGVGPFA
                     MGWVTDNVFGDPMKIHYSMALVTLVMGSLGTALIAYGLKAFRGSLSRVTWG"
     gene            complement(21820..23484)
                     /locus_tag="PKN91_02200"
     CDS             complement(21820..23484)
                     /locus_tag="PKN91_02200"
                     /inference="COORDINATES: protein motif:HMM:NF005760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HNS26911.1"
                     /translation="MNGAQALMKTLADAGVSVCFSNPGTSEMHFVAALDHEPRMRAVL
                     TLFEGVATGAADGYARMTGKPAATLLHLGCGLGNGLANLHNARKGRVPVVNIVGDHAT
                     SHVKYDAQLQSDIETVARNVSTWVRTSASTRALGVDTAQAVAAAMGPPGQVATLVLPA
                     DVSWSEGGEPAQRLPVAPPPVTDEATIDAIVQAIRSGGKTALLLGARALREPGLLAAS
                     RIAAHTGAGLFAEVFPTRIERGAGLPHVERLAYLAELASVQLHGLEHLVLVDAKSPVS
                     FFAYPGKASDLVPEGCTVHALATPTEDALGGLQRLAARLGVDAAAPVLQAPVRTERPR
                     GKLTAEKVCKAVGHLLPERAIIVDEAQTSGVMLPHYTRGAARHDVITLTGGAIGQGLP
                     NAVGIAIACPDRPVLALVGDGSAMYTIQALWTMAREKLNVTTLVFNNRSYAILNMELQ
                     RMGATGGGIGAGEKARAQLELGNPGLDFVQIGAGMGVHAVRATTTDELVDALGYALRA
                     PGPHLIEAVVPSTFSGLKLRLLPHVLGSLARLPQPLAMAIKRKVSP"
     gene            complement(23481..24662)
                     /locus_tag="PKN91_02205"
     CDS             complement(23481..24662)
                     /locus_tag="PKN91_02205"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014719250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HNS26912.1"
                     /translation="MALRFPATAQDYRRLARVRLPRFLFDYVDGGAGDEQTLRANEAA
                     WARLRLRQRVLVDVDGVDTSATLAGQACAMPLALAPLGLAGMIARRGEVQAARAAEAA
                     GVPFILSTVGICPLDEIQRAVAKPVWFQLYMLRDRGIVRALLQRAWDAGCRTLVFTVD
                     LPLPGIRHRDIRHGLGARTFRARMLRIADLLSHPGWAWDVGVRGKPHVFGSLAGQLPA
                     ARDLDAFKAWVDTQFDPSVTWADIDWLRGQWPGVLLLKGILDEDDARAAASAGAQGLV
                     VSNHGGRQLDGVAATAARLPAIVEALGAQVEVLVDGGIRSGVDVLRALALGAHGVLVG
                     RPWAWAIAAGGERALAGMLARWQQELRLAMTLAGVRRLADIGAGQLEPSGFEDHEEAM
                     R"
     gene            complement(24662..26275)
                     /locus_tag="PKN91_02210"
     CDS             complement(24662..26275)
                     /locus_tag="PKN91_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNS26913.1"
                     /translation="MAQARRTDVLVAGGGLAGLVTALECLRGGLRVTVVDRDTPARLG
                     GLALWAFGGMALIDTPLQRRRKIADSPQRALRDWMRFGELDEADMWPRRWAELYVEHS
                     RARVYDWLLAQGIKFFPTVTWVERGYAGEGNSVPRYHIVWGTSRELVRRMIQGMNAVG
                     GGGRLTVLSGHRVVSIDSAGGRISGVQAEREATGEVLRIEAPVVALAMGGINGSLEQA
                     IANWPADRPRPAEMLNGAHPFADGRLHHCVASELGGRLTHVGEMWNYAAGIPHPQPHF
                     PGHGLSLIPCKSALWLDHRGRRIGPAPLVTGFDTRDLCRRVAALDGAWTWHLLNRRIA
                     AREFAISGAEYNPRIRDRQFLRFVLETVLGNNCLLERMQRESRHFLVDDSLEGLAAKM
                     NVLTGTDDIDPATLQATADAFDAHFGHGSSPDGDEQVRRILQARQWGPDRLRTCRPAP
                     LQAKGAGPYIAIQTRLITRKSLGGLQTDLQSRVLRADGSAVPGLYCVGEAAGFGGGGA
                     NGKRSLEGTFLPGCILTARAAADSIREGG"
     gene            26419..27831
                     /locus_tag="PKN91_02215"
     CDS             26419..27831
                     /locus_tag="PKN91_02215"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HNS26914.1"
                     /translation="MDTRADTPSAELRPTLERLRAAWQANKPDYAQRRDDLRRLRDAL
                     KRRLDEMVRTIAADFGHRSPDESRIADGMTVLNDMDHLIRHLRGWMKPRRVGVGWHFL
                     PARAQLRPVPLGVVGVIAPWNYPVNLALVPLATAVAAGNHAYLKPSEHTPRTSAFLRD
                     LLAEVFPADRVAVATGGAEVGAAFAALPFDHLVFTGSTAVGRKVMAAAAHNLTPLTLE
                     LGGKSPALVCDDYPIDNAAARIATGKWFNCGQTCIAPDYVLVPAGRRDALVEALRREV
                     RVRYGADFANLGDYTRVINDGQYARLQGYLDDARRRGIDIVTLGGSADPAQRLLPPTV
                     LLEPGDDAQVMQDEVFGPLLPVKSYRTLDEAIAYINVRDRPLALYPFSHDRARIEKIL
                     HDTIAGGVSVNDTLLHFAVSGLPFGGVGASGMGAYHGRAGFDAMSKLQPVLWQARRTG
                     GDLLKPPYSKAKWLIDLIVR"
     gene            27828..27971
                     /locus_tag="PKN91_02220"
     CDS             27828..27971
                     /locus_tag="PKN91_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26915.1"
                     /translation="MSARARHRQAAVVSSERYLMALGGETILLRRVRYFPHHLLGQTA
                     GAS"
     gene            complement(27968..28699)
                     /locus_tag="PKN91_02225"
     CDS             complement(27968..28699)
                     /locus_tag="PKN91_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26916.1"
                     /translation="MITNTASGTNLHEIASGIYRINTPVEIAGGLGFNFNQYLVVDEE
                     PLLFHTGPRRMFPLVAEAIGKVVPLERLRYVAFSHYEADECGSLNEFLAAAPAAAAVC
                     GQVAAMVSVDDIATRPARALADGEILRTGRHAFKWLDTPHVPHGWECGLMMEMETRTL
                     LCGDLFTQGGDGSVAVTDGDILSPSEAFRRAMDYYAHAPQTGATLERLAREQPQVLAC
                     MHGSAWRGDGAALLRALASSLASAS"
     gene            28803..29381
                     /locus_tag="PKN91_02230"
     CDS             28803..29381
                     /locus_tag="PKN91_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNS26917.1"
                     /translation="MPRSLLLVELDDGALARRISETGAAPDSAAEAELYRRLAPRVRL
                     YGLRHLRDLQAAADLVQQVLLMTLERLRAGKVRDPESIASFVLGSCRMTVLEMRRGTR
                     RREALLETWSDAAEPCEAPEQEALDPHRLAGCLEALSERERSVVVLTFFADRQADEVA
                     SELAISGGNVRVIRHRALGRLRKCMGGGEEVQ"
     gene            29378..29935
                     /locus_tag="PKN91_02235"
     CDS             29378..29935
                     /locus_tag="PKN91_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNS26918.1"
                     /translation="MTCRSPVALDALVAYWLGELPQEREAALEEHLFGCTQCTGQLEE
                     LAALATGVRATVLDGKVSMVVSEPFVDAMKDAGLSVREYQVEPGGSVNCTIGTNDDGV
                     ISRIRAPLAGVQRLDVVRMLGASGPEERLTDVPFDADSGGLVIVPSAAWLKAMPAFTM
                     RMRLIAVNDAGEKQLAEYTFNHSPS"
     gene            complement(30121..32163)
                     /locus_tag="PKN91_02240"
     CDS             complement(30121..32163)
                     /locus_tag="PKN91_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNS26919.1"
                     /translation="MASESSIVAHGLSRDSVSIGHWRAVQAWLGPYGLMLRFMLPVLL
                     LLSASRLLLMAWQWRRVAETDAVAWMLAQGVRSDLITIGVCIAPALLALPMLLMMKRM
                     AWWEQLCKAWFALCFIVISFLELATPQFLIEYDGRPGTMLLQYLAYPGEVSAMLWQGY
                     RGTLFTTLLVLGGATWWSIRYFRVAEAPAIHLRALRAALLWPVMVILAVLMIRSSLEH
                     RPANLASFLFCDDAMVNSLVANSAYTVLSASLAMRAEDQTGTSYGSMPHEVIVRRVRA
                     DMGVPPARFTSDQLPTLHRQQPSVQRTRPLNLVIVLEESLGAGFVERLGGLPVTPTLN
                     RLADQGIWFERLYATGTRSIRGIEAVVAGFPPTPALSVVKLPNAQRDFTTLASLLRRS
                     GFRSEFVYGGESHFDNMRGFFLGNGFNAVVDRSQFNDPQFVGSWGASDEDLFAKAHER
                     IEALHGQQQSFFLLVFSSSNHTPFEYPDGRIEQFDAEKHTVNNAVKYADYALGQFLDQ
                     ARSSDYWRDTLFLVVADHDTRVHGSALVPLDKFHIPGVILGADVAPLKVQSIVSQIDL
                     APTLLSLMGVDNTHPMPGRDLTRTLPEFGNSSGPPPRAMMQYEQSYARLQGNVLTVLL
                     PNGTARDFSVGDAGRQLTPIEHNDPERHRDALASVLMPAWIYRERRYRVEHALLSN"
BASE COUNT         5151 a        11010 c        10931 g         5199 t
ORIGIN      
        1 gaaagcgttg gttccattgc tcgcgctgcc gccgcgcacg cagcgccgcg catgcaacca
       61 acgctttcta ccgtcgcgat cagctcacct gggtacgcgg tgaggaccgc gtcgagcgct
      121 actcactggc cggcgcgcag cgtttcgggc aggatttctg ccgccgctgc ggctcgcccg
      181 tcgcgcgcgt ggtggcatcg accggctacg tcgtcgtgcc gtgcggcagc ctcgatgacg
      241 cgcccggcgt cgagccgcgc ggacacatct tcgtcggcag caaggcgccc tggcacgaga
      301 tcaccgacga cctgccgcaa tgggaagcct tgcctgcgtg agcctctacc atgggcgcca
      361 gtgtgcgccc tgccggcgga atcacgcatc ggctatgctc gccggtatcg gcacaaggac
      421 aacaacatga aattcccgtt agcccgatgt gcactgatcg cagccctggc aatcgcgaca
      481 tggccggcgc ccctgctcgc ccagatcgcg ccgtatcagg tgaaggcccg cgaggtgtat
      541 gcgcgcatca tcgggtttcg caccgcggcc ggccagaatc agaccggcgc catggcggag
      601 tggctcgccg atgtccttag agccggcggc gtaccggcat cggacatcgc aacgctcgaa
      661 gccggcggcg cgactgcgat gctcgtgcgc attccgggtc gcaaggcgcc gggccgcacg
      721 gcggcgcgcc cgatcctctt ctccgcccac atggatgtcg tcgatgcgcg cccggccgac
      781 tggcagcgcg atccgttcac gctgatcgag gaggacggct acttctacgg ccgcggcacg
      841 atggacaaca aggcaggtgt cacggcgctc gtgtcgacga tcctgcgttt cactgcggag
      901 cgactgcacc cgacgcgtac gctcgtgttt gccttcgtcg gcgatgaaga aacgggcatg
      961 gagacgaccc gcgccattgc cgcgcaccca tgggtgaagg atgccgagtt cgcgatcaac
     1021 gccgatgccg gcggtggcgc gctcgacgat gccacgggca agccgctcat ctacctcgtg
     1081 cagggcgccg agaagactta cgcgacgttc gacctgacgg tcacgaaccc gggcggacac
     1141 agctcgcggc cccgcgccga caatgcgatc taccagctcg cgcacgcgct cgacaggatc
     1201 gaggcgcacc gctttccggt gatggcgaac gagctgacgc gcgcctacct cggcgcgatg
     1261 ggccgcgcgc aaaccggcgc ggtcggcgat gcgctgcggc gcttcgcagc gaatccgggc
     1321 gacgccgcgg ccgccgatgc gctcgcgcag tcgcccgagt tcgtcggcac gacacgcacc
     1381 acctgtgtcg cgacgatgct gcaggcgggc cacgccgaga atgccttgcc gcagaaggca
     1441 accgccgcga tcaattgccg cattttcccg ggcgtcgcgg tcgatacggt gcgcgacgaa
     1501 ctcgtccgca cgatcgacga cccggaggtc cgtgtcgtgg tgcgcggtac gccgaccgcg
     1561 agcccggtgt cggaaatgcg ccccgacgtc acggcggcga tcacgcgcgc ggtgcacgcc
     1621 gtccacgcgg gcgtggcgat cgtcccgtat ctcgaatccg gcggcacaga cggcaaggtc
     1681 tatcgaaccg cgggtatccc gaccttcgca tcctcgggtc tcttctcgaa gccgtccgaa
     1741 atgttcgcgc acgggctcga cgagcgcctg ccggtcgcgt ccttctacca gggactcgat
     1801 cacatctatc gtcttgccgt ggatctcggc ggtgtccgcg ggcggcgctg atatcggagt
     1861 agctggcccg cgggcgatat cctagcgagc actccggcgt gcggcgttgc tcgacccgtc
     1921 tgccgacgcc gcaatcccgc gcccgagtgc tgccagcagc tgctcatacg gctccaggaa
     1981 tttctgcacg ccttccgctt ccagtcgtcg cgcaacatcg tcgagttcga tgccgagcgc
     2041 cgcaagcagg cctggcaagg cgcgtgccgt gtcgagatcc tccgcgatgc gcacggcggc
     2101 ctggccgtgg tcgcggaatg cggccagtgt cgtcgtcggc atcgtgttga tcgtctcggg
     2161 cgcgatcaac gcttcgacgt agcggacatc ccgataggcg tcgtccttgg tggaagtgga
     2221 ggcccagagc aggcgctgcg gcagcgcacc tgccgccgcc agtgaccgcc agcgcggcgt
     2281 ccggatcaac cgctcgtact gctcgtaggc gagacgggcg ctcgcaatgg cggcctgccc
     2341 gcgaagcgcc tgcgcgtccg gcgagccgat cgcatccagt tgacggtcca cgaacgaatc
     2401 gatgcggctc aggaaaaagc ttgcagcgga cacggggaga gatcctggat cgccgccgga
     2461 ctggatccgg tcctcgagcc cagcgatcca ggcctccgcg accgccgtgt agcgcgacac
     2521 gctgaagaga agcgtgacat tcacgttgac gccggccgcg atcagcttgc ggaccgccgg
     2581 cagcccggcc tcggtcgcgg gcaccttgat catgaggttc gggcgcgcga ccgcttccca
     2641 caggcgccgc ccctcggcca ccgtcgcatc gcagtcgtgc gcaaggcgcg gagagacttc
     2701 gaggctcacg tagccgtcgg tccgggcggt cgtgcggtga atgtcgagca gcaggtccgc
     2761 ggcggcctgg atgtccgcga tcacgagcgt ttcgtagagg tcccgcgcgg tcaggcgaga
     2821 gccggccagc ccggcaatcg ccgccgcgta atcgtcggtt tccgtgatcg ccttggcgag
     2881 aatcgcggga ttcgacgtca cgccggtcac accatccagg gcgatgaggc gcgacagtgt
     2941 gccgtccgtc agccacgcgc gccggagatc gtcgatccag acgctctggc acagatcttt
     3001 cagggatagc aacgggttcg tcgtcgccat ggctagatgt tgtcggcttc cgtggaagtg
     3061 cgcgcacgca cgtcggcgga gcggccccag cgccacccgg ctacggccgg catgtcctcg
     3121 ccatagcgat gaatgtatcg cttgtgctcg atccggcagt cgcgcatcgc ctgcttgacg
     3181 tacgcggcgc gcgagccgag gccgggaacc cgccggatca cgtcctgcac gagacggaag
     3241 cggtcgaggt cgttcaggac gaccatgtcg aacggcgtcg tcgtggtgcc ttcctccttg
     3301 tagccgcgca cgtgcaggtt gccgtggttc gtgcgccggt aagtgagctg gtggatcagc
     3361 gacggatacc cgtggaaggc aaagatgatc ggccggtcgc gcgtgaacag gacgtcgaac
     3421 tcgtcgctcg tgagaccgtg cgggtgctcc tcggccggct gcagcttcat gaggtcgacg
     3481 acattgacga cccgcaccct gagctcgggc aggtgctggt gcaggagctc gacggccgcg
     3541 agcgtctcga gcgtcggcac gtcgccacag caggccatca ccacatcggg gtccgcgccc
     3601 tcatcgttgc tggcccaggg ccagatgccg acgccggcgg tgcagtgccg gatcgcctcg
     3661 tccatcggca gccattgcgg cgcaggctgc ttgccggcga cgacgacgtt cacgcagttg
     3721 cggctgcgca ggcattggtc cgtcaccgcg agcagcgtgt tcgcatcggg cgggaggtag
     3781 acccggatca cctcggcctt cttgttcacg acatggtcga taaagccggg atcctggtga
     3841 ctgaagccgt tgtggtcctg gcgccagacg tgcgagctca acaggtagtt gagcgaggcg
     3901 atcgggcggc gccacggaat gtggttgcag accttcagcc atttggcgtg ctggttgaac
     3961 atcgaatcga cgatgtggat gaaggcctcg tagcagctga agaacccgtg ccgcccggtg
     4021 agcaggtagc cctcgagcca gccctggcac tggtgctccg acagcacctc catgacgcga
     4081 ccgtcgggcg agagccgatc gtcgcccgcg tgaatctccg ccacccaggt gcggtccgtc
     4141 acctcgagga tgtcctgcca gcgattggag ttgttctcgt cggggctgaa cacgcggaaa
     4201 ttcgcatgcg gcaggttccg ctgcatcacg tcccgcaggt acctgccctg cacgcgcgtc
     4261 gattcggcgc tcacctcgcc gggcgagacg acggccacgg cgtagtcctg gatcgccggc
     4321 agctcgaggt cgcggagcag cacgccgccg ttcgcgtgcg gattggcgct catgcggcgc
     4381 tcgccgcgcg gcgcgagtgc ggcgatctcc gcaacgggcg caccgcgctc atcgaacagc
     4441 tcgtccggcc gatagctgcg cagccattgc tcgagcactg ccacgtgctc cggcttgtcc
     4501 atgtcgccca tcggcacctg gtggctgcgc cagctgcctt ccgtgcgctt accgtcgatt
     4561 tctttcggcc cggtccagcc cttcggggac ctgagcacga tcatcggcca ggccggccgg
     4621 cccccgaaac cgcgcgtacg tgcgtcgtgc tggatcgcgc ggatctcgcc gatcaccgca
     4681 tcgagcgtgg ccgccatcgc ctggtgcatg cgcccggggt cgtcgccttc gacgaagtgc
     4741 gggtggtagc cgtagccttc cagcagctgc gtgagttccg cagccgggat gcgcgccagg
     4801 atgcagggac tcgcgatctt gtagccattg aggtgcagga tcggcagcac gcagccgtcg
     4861 cgcaccgggt tcaggaactt gttgccgtgc cagcccgtcg ccagcgggcc ggtctcggcc
     4921 tcgccgtcgc cgaccacgca ggcgacgatg aggtccggat tgtcgaacgc ggcgccgtag
     4981 gcgtgggcga gcgaataacc gagttcgccg ccctcatgga tcgacccggg cgtctcgggc
     5041 gccgcatggc tcgggatccc gccgggaaag ctgaactgcg tgaacaggcg cttcagcccc
     5101 gcttcgtcca ggctgacatc cgggtacagc tcgctgtagg tcccgtcgag ccaggcctgc
     5161 gcgacgacgg cgggcccgcc gtggccggga cctgcgatgt agatcatgtc gaggtcgaac
     5221 ttgcggattg cgcggttgag gtgcgtatag atgaagttga gccccggcgt cgtgccgaaa
     5281 tggccgagca ggcgcggctt gatgtgctcg cgccgcagcg gttcgcgcag caacggattg
     5341 tcgtacaggt agatctggcc gaccgcgagg taattggacg cgcgccagtg cgcgtcgatc
     5401 gcctggagtt ccgccgccga cagcggcggc gcatcgatcg tgtccgaggg tccggctccc
     5461 cggcgggccg tacgttgatg cctgttgctc attgcacctg cgtctccgcg gccgaccatc
     5521 gcgcgtgccg gccggggtgt caatccgcag catgtcgtgc gctcgtccag gcagccaaca
     5581 cccgtcatgt tgacctggcg cagcagatcg ccctgcccct gcccgccgcg cgccagtccg
     5641 acgccatcgc ccttctacat cgtgcgttgc gacggacgat agtgcgggaa accaccccgt
     5701 acaggcactc actgcagctc gtcggccaga tcgcggagcg gctggctgct ggggagcggg
     5761 cgaagtggct ggctcagctg cacgtcgagt gcaaggcaaa gcgcaacttc gtgcgcgatc
     5821 tgcctgggtc acctcacccc gactattctc agcttgaaga ctggaacggc tccagccctg
     5881 caaaagcacc ctccgttcag gccacgaccg gacgcacaga tttccggtgc gccatgaatg
     5941 cgactacacc gccggcgatg agcaccgccg tgaacaggat ggcttgcttg gcgaagaaca
     6001 gcggtacgat cccgccggca agcatcacga ccgcgcccgc catcaatcgg ccgcggaagg
     6061 cgataatgcc gcccacgatt cccgccacca gactgccgag cagcagcagc ccggcaatgc
     6121 ccatggcctg cagctgatcg ccttggccca tcaattgcgc cgtcatcttt tgctgctccg
     6181 tgagcgcacc gatgtcgctc agccatttca tgccgatccc gcccgcgatc acgccgccga
     6241 ggatgcccag aaccagtgcc gccttcttca tgcttcgctc ctccagttgc gatcccgacc
     6301 atggaggcat catggtgccc gccgcacggg cggggcgcat cgacagatag cgcgacttag
     6361 cggccggtag ggaaagatga ccgcggtcag ccccgatcgg tgagtgccac gagatccggc
     6421 agccgcgcgt accctctgca ggtctcggtg gctcgtcgca ggctcgcccg cgccatgtcg
     6481 agatcgccgc gctgcaggta gatctgcgcg agccggcgca tatcgatcgc cgaggcgagt
     6541 tcggcgccga tcgagtcgat ctcatcggcg atgccgagca ggaccttggg cggcaccgtc
     6601 cggggatcga gctgatcgag catcgtgtaa tagtacgcac gtgccgcgcg atcggcggct
     6661 ggcgccgtgc cgcgcagatg gtcgcgctcg gcgatttccg ctgcgcgcag gtacgcggcc
     6721 acggccgccc cgcggtcgcc gacctggcgg tacgaatccg cgagattgcc ccacatctcg
     6781 tgaatttccg gctcgccacc ggacaccgcc gcgatggccc gctgccgcag cgccgccgag
     6841 cgctcgaagt cccccaaaaa gtagtacacg gtgccgagga attcatcccc cacgtacgag
     6901 gccggattga gcgcgcgggc gcgcaggtag atgtcgcgcg ccttctcaaa tgcgccgtcg
     6961 cagaggtaga agctgcctag attcgccatc acatactcgt tttcgtcgcg ggccagcgcc
     7021 tgctcgctga cggcgatcgc tgccgcgacg tcgccgtcga accactccat cgtggcgagc
     7081 gcaaacaacg gcttccagac ggcagggtcc acctctcctg ctcgtcgggc cgtgtccttg
     7141 gcggactcga gcagcgcacg ctccccgctt gcacgccagg cctgcaggcg tgccgccgcg
     7201 agccccgtca acacggcggc gtccagcggg tgcgcagaag cggtccgctc gtagagtgca
     7261 atcgcctcct cgttgcgccc ggtacgactc aggaaatgcg cgtgcgcggc ggccacgacc
     7321 ctgtcctccg gatcgagctg cacagcacgg ccacaggcga cagccgcatc cttcagcgca
     7381 cgctcctcat tgtccatcca gtgcccttcg agcaacgtct ggcacaagcc cgcatgcgct
     7441 cgcgcataat tcggatcacg tcgcagcgcg gcttcgaagt aaccccgcgc gcggcgcaga
     7501 ttgagctcgt tggacgggcg atcgaggtaa tactcccctc gcaggtactc gtcctgaacc
     7561 ggcgcgagcg gaaaatgtcc gcgcgcgcgt gacggcaccg gcaatccggt cgcccgacgg
     7621 atggcaagcg atgccccgcg ggcgatgtcg tgcagcaaac tgctcagcgc cacggcgggc
     7681 agactctgtg accagacctg ctggcggacg ccatcggccg acaggaacag gcgaaccgcg
     7741 ctgagccgcc cgacggtgac aatctcgccg tcgagtgcgg cgtcagtccg cagccgcgtc
     7801 gacgcggaca ccgggtcgag atcgctcgtg agcacctcgg cggtcgcggt ggccactgca
     7861 aacgtattgc cgagctcagc gcgcaacatt ccggtcagcg cgccttccgt gtcgcctttg
     7921 atgggcagga cgacgagcga ctcactgccc aggccgagaa gcgcgcggct cgcggtggaa
     7981 gtcggcgaga ggtcccgctc gcgcggcccg cgcgtgaacc accaggtgga ggccgtgacg
     8041 acgacgagta ccgcgacgct cagtgtccac aagcggcgac gggacggatg ggtcgggggc
     8101 ggcaaatgcg cgggctgcgg gtgctcggcc tgcggatgca ccgacgccac tgccggccgt
     8161 gcctcctctc gagccgtctt gtacagactg taggcatcga cgcccagagc gcgcgcgacg
     8221 cgctccagtg tctggggctc gaccggccgc tcgcgaaaca atctgctgac gagatccttc
     8281 ggcgccgcat ccagaccttc tagatcagcg atgcgctcag ctagcgccgc ctgggtccgc
     8341 aaaccggctt ccgtcagcgc gtgggcaagc ctcacgcgcg aggcacgcac accgcgcttg
     8401 cgtcctgtgc cttccgtgcc cgtcatcgcc caccctgcat cccgcttgtt cttgtccctc
     8461 caaacccttc tggatgttta tagcacagcg tttcggcagt cgtcgcgtgc aggcggagag
     8521 cgccgcctgg gccgctgcgc gtcccgatga gtcccaaagt cccgttatgt gcgtttatct
     8581 gtcgatgccg aaatccggcc ggcgtcgaga aaatgtcgat cccggcactc atcgcgaggc
     8641 atcttcatga acagcactac ggcaggcgtc ggcgccatga ccatcacgct gtgcatcatt
     8701 gcggccggat gcggcgacac tggcacaggt acggattcga gtccgccgtt cgcgggcagc
     8761 ttcgcgccgc agttcccgcc gggtgcatcc cggctggatc gcgtgggcgt ggacacccgc
     8821 tgggagttct cgcccagcgg gacggtcatc actcgcggcc cccaccgcac ggtgacctgg
     8881 cgctacgaga ttcgcggcag cgagattcga ctcaccggcg tggatgaccg caatcagggc
     8941 gagaggcgcc tgtttacgcg tgccgatgaa cattgcatct gggacggtca cggcagctcg
     9001 gtggacgtga aattctgccc ggtggagtag acgcggccga cctctgcagc aagcagagaa
     9061 ctcaaaccaa tatgaacaca aggtgaaaca gatggcgatg atgaaattgc tgatcggggc
     9121 gaccctgttc gtcgtgctcg ccggctgcgg cggaagcgcg ggcacgacgg acgacaggac
     9181 tgcaatcatc gatgcctgca atacccagca aggcgcagga tggcttcgca aggagtacgg
     9241 tgagaactac tgcgagtgct atgcggatac ggcgcagcag aagctggatt cggactccta
     9301 caggacactc gccgccgcct cccgcgaaga gctgaaggcc gcggatgatg aagccgcccg
     9361 cgaaaccatc gcccgacgca acacgggggt ttactccgag gcgtcacatc acgcgcaatc
     9421 ctgcaagaga acgtaatcgc actcatgccc ggaacgtgtt gctggccagg caagaagagg
     9481 gtcgcgcgat gccgatagag aaatgccatg agggcaggaa gctgctgtac aagtttgccg
     9541 tgagcaggct ttccggaacg gtcctgggcg agactacacg cagcgaaacc accgtcagcg
     9601 gcaagatcta cggcggcgga acgaacagca cctatggcac cgtcgccccg gtacagggaa
     9661 ctatcgaatc ggaaacgacg cgctatcaga ccgtctttct gagggacgac gacggctccg
     9721 agcacgcgat cgagctcgtg gatctactgg ttccctgtcg ggaggggcac aagctgacgc
     9781 tctggcgtct tgggaaggat ctctgggttc aggcccggaa tcagagcacc aaccaggtct
     9841 acaggtacgg aaagcttaag aagctgctgt atcccaaggt tgcctatttc gcgatcggta
     9901 tcctgttcgg catctatgcg gtcgatctcg acaaatccgg cctgctgggt ctgctgtttg
     9961 ccattctggt cggcctggcc attgcgacga ttccatgcgg gctgatcgcc tggatgcgtg
    10021 aacgggcgat cttcgaggca accggcgtgc aggagcaaag tggaagccgg acgccagttc
    10081 ggtgaaatcc tcagcgggtt tttcgttgcc tctcatcgtg tgcccgcgct cccggcgctc
    10141 ggcagccgcc aggtcgttca ttggactgga cgccgatcac gggcattgct gaggaccgtc
    10201 gccggccacg ataatgctgg acgtcaattc atcctgtgga gatgccccat gcccgtgtac
    10261 ctggtatccg tcgttgagct cacgaatccg gggccgaaca tgaaacggta tgccgaaaga
    10321 tcggctgagc tgatccgcca gcatgggggg cgctacctgg tgcgcggcaa ggcgtcgcaa
    10381 gtggtctctg gagagctctt caacaatcgg gtgctgatcg tcaccgagtt tccagcgctc
    10441 gagaactacc aggcattccg caacagtgat gcctaccaga aagaatgccg gcccctgcgc
    10501 gagggtaccg gtatctacga taccggggtg ttcgatgccc cgccgccagg gatggcctga
    10561 cggacatcgc gatcgtccgc cggacgccca ccgggtcacg acgccaagct tcgacggcgg
    10621 attcgtccac gatggccact cggtgcaact gggcgatcgc gtgatgcgcc accgggcacc
    10681 gcggtgctgg tgctgctggt ctccgtcccc gtcggtggca agcggcgagc gccgcggcga
    10741 tctccggcgc gtcagcgcgc agtctggcac tcggcgatct cgtcctcggt cagcccgagg
    10801 tcgcgtgcgg cagccgccag gtcgtccgca gtggccttgg ccgtccgcgc ctcgccgatg
    10861 gcgcgcgtga cggcctcgcc gcccaggcgc cctgcgttgc gtgccacggc gcccatgagc
    10921 cggcccagac cctgtttctt cttctgtgcc gcctgtcgtt gccgcgcctt ttcctccagg
    10981 caggccttgt atgcgctctg gtcgccggcc tggcccgcag aggcggccgg cgcggtcgac
    11041 gccggcattg tcattggcgt tggcatcggc gttggcattg ccccagcgcc acccattgag
    11101 gatgcggcag cggacagttg cacccgccgc agcgcctcgt cgtccggatt gaccaggatc
    11161 tgctgcacct cggtgacgac ctcgatgccg ccgccggagg ccatacgctt catcgtctcc
    11221 atctgcggcc ccatgcgttg catgaccatg tcgcgttgcg caggtggcat ctgcgcgagt
    11281 tggcggtcga gttcctcgag cttggcctgc gactcgcgca gttccttctg ttgcgcctcg
    11341 gtcatcgctc cctggatccg catgacctgc cggaagggtt cgtacatctt cgagccgggc
    11401 acggcgcgat actgggtgtt gacgcgctcg atcgtcaccg gctgggtctt gccgtcactg
    11461 gtgtgcgtgc cggccatggt gaatttgatc ggcacgcagt tcccggcatc catccacacg
    11521 tcgagctttt cgatcaccat ctcgccgccg ccctgcgtgg cgtgccgctg tccgaggccc
    11581 tcggcccgca ggtgctcggt ggcgacgccg tcgacggact cgctgccgac gtgtcgcagc
    11641 cgtcgcccca ccttgcccag ggcggcggaa tcggcggccg cttcggcgac ggccgcatcg
    11701 cgctcacgct ggtttgcgtc ctgcgcctcc gcggccgcct cgaggaaggt gcccgcccct
    11761 cccatcatca cgctcgggtc gggcgacgcc cacggttcgc caccgtggtc gagcatgccc
    11821 accggcaggc cggaggcctg catctcgttc gagagcccga taccaagccc cttggccttg
    11881 cccgcgatca tcttcagttc ggcgctcgag tacgggctgt cggcggtcat cggccgaaat
    11941 cccggcagcc gctgcccatc gggcccgacg acctcgaagc gctcgtatgg cagtgcgacc
    12001 tgctggccca tcatcgactg gacgacgacg tagtgcgtca cgccgtccca gcggtcggcc
    12061 ttgacctgct cgcacttgcg cagcagcgcg gctgggtcaa cggctcccgc cggggatgcc
    12121 ccggcgagca tcagaatgac gaccgacagc gaagccttgg cgtggcgcat cgttgaatcc
    12181 ccctttactg agttcctata gcgggattgc gacgcgaaac cgccatccag gacggcatcg
    12241 agtgcggcga cgggatcagc cctcgacggg cacccggcac agctctggat agggctccgg
    12301 caggcttgcc gaaccgatca gggcatggga gctggcgatg agtgccccgc cctcggccgc
    12361 ctgcccgcgg cgctcgagtg acacgccgac gaggcactgc gcgacgcctt gcaggtggcg
    12421 cgccgccggc gtcgcctgca ggcgcgcgag cgcttcgcgg gccgccgcct cggcctccgc
    12481 cagctcgccg cgccgcagct gtacgtaggc gatcgacagc agcggcactg ctatcacgta
    12541 gttatcctca ttaagcacga ccttgaaggt ctcgtaggcc ttgcgatgca ggggcaacga
    12601 ctcatcgtag cgtccctggt gtgtgatcgc gctgccgaag ttctggtagc tgtcggcgac
    12661 ggcgctgtgc tgctcaccgt acttcgttat cttccgcgcc agcagttcac cgtggagtcg
    12721 ctcggcaccg gcgaggtcgc cttgcccgtg gtacaggtag cccaggttgt tgagcgcgtc
    12781 tagcgtgcta ccgtgattcg ggccgagcct tgcctcgagc accgggatgg ccgcgagaaa
    12841 gtgctgctcc gacgcatcat attcgccgcg gttagtcaga ctcgacgcga gcctcacctg
    12901 taccagcgct gcctgcaggc tgtcggcgcc gaacacccgt cgcgcgacct gctccgcctc
    12961 gcgcagcggg tcgaagtcga tcggcacatc ggcgaacgac cgatgctgct ccagcacgat
    13021 gagcgcctcg acgagttcat tgggatgcga cgccggttcg gcacgcagcg tcgcgacgcc
    13081 ctgcagcagc agttcatggc tgcgatcgag attgccacgc cgctcctcgt gcagaccgga
    13141 ggcgatccgg gccacggcga ggtccgctgg cgacactccc cgcgacgaac gcgcgatctc
    13201 cagctgccgt tccagcagta cgtcggctcg ttccatgtcg ccggtcgccg cgtactggta
    13261 tccgagcgcg cgcaggctcg acgccacctg cgccgagcgc ggcccgtgca actccgtctc
    13321 gagcgcgagc gcctcctcgc gcaggtcgat cgcctcggcg tgctggtcga ggcttgcata
    13381 gacgtcggag atggacgcaa gcagggcggc gcgcagctcc ggctgcgcgc cgagttcctc
    13441 gcgcacgcga cgctgtccca gctcgagcgt gtcgacgacc cggatgtcgc ggccgcgggc
    13501 gggatccgcc ggcgcgaacg gatccgggct gcggaacaac tccaccatga actgctgggc
    13561 cgcggccgcc agctgccgct cgcgctcgag ccgcgcggaa tagacggtga gcgtgacgac
    13621 ataggcgatc gtgccgagca caacgacggc cagcagcggc atcaaccacg ggttgcggcc
    13681 tgccagcttg cgcaaccggt agctgaagcg gtcaggctgc gcctgcaccg gacgaccggc
    13741 gaggaagtgc tcgagatcga ggcgcatctc gtccgcggag gtgtagcgct gcgcgggatc
    13801 ggcgtgcgcc gcccgcagcg tgatggcatc gaggtcgccg cgcacctgac gatacaacgc
    13861 cgtgccgcgc agccgctggc tgggtggcgg cacctggaag gctacggcgg atgggtcgcc
    13921 gtcgaacggg aactccccgg tgagcagctc gtacagcagc agccccgcct ggtagatgtc
    13981 ggatcccgtg gtgacggtgg cacgcgcgtg ctgctccggg ctgccgtagc cgggcgtcag
    14041 cagcgcgacg ccggtacggg tgtgcagcgc gccggtcggg gacgggcccg gctcgagcag
    14101 cttcgcgatg ccgaagtcga gcagcttcac ctcgccttcg gccgacacca gcacgttcga
    14161 tggcttgacg tcgcgatgca ctacgaggtg gctgtgcgcg tggtgcagcg cgcgcagcac
    14221 acggatcagc agacgcaggc gatcgcgcag ctcgagcccg cgctcgctgc agtggtcggt
    14281 gatcggccgg ccatcgacgt attccagcac gaggtagggg cgaccgtccg gcagtgcgcc
    14341 gccgtcgagg aagcgcgcga tcgacgggtg ctccagcgac gacaggatct gccgctcggc
    14401 gcggaagcgt gcgacgacgt cctccgtgtc cagcccgcgt cgcagcacct tgaatgctgc
    14461 ctgctgcacg aattctccgt cgtcccgctg cgcgaggtag acggtcgcga ggccgccgcg
    14521 cgccaggcga cgctcgatcc gccagttctg aaaccgctgc ccgctgaggt cttcctcggc
    14581 tttcgcgtcc gacgcgagca gcgagcgaaa aaggtgctcg cgcgccgcct gtatctgctc
    14641 cgcgaacgga tcgccctcgc gctcggccgc gtcgagcaag gcctcgacct cgcggcgcaa
    14701 cggctcgtcg gcgcggcact gctcgtccag gtagcgtcgc cgctcgcctc ccctgcgctc
    14761 gagggcgccg gtgtagatgc ggtcgagcgc cccgccgcac ccggtcgtgg gggaatcagc
    14821 cgccaccgga ctgcagggcc cgcttcaggt agcccttcgc cctcaggacc tcgcgctcga
    14881 ccgtgcgctc ggacatcccg agcgcttccg cggtctcgcg ggtgctgagt ccggcgaagt
    14941 agcgacactc catgacctgc tccagacgct gatcgatccg tgacagttcc ctaagagcgt
    15001 cgtccagcgc aatcaccgcc acgctgtcgt cgtccgtggc gggacccgcg tcggtgagcg
    15061 tgagcggccg caggccgctg ccgcgcttcg cggcttcgcg ctgcctcgcg tagtccacca
    15121 gcaggtggcg catcacggca ctggcggccc gcaggaagtg ctcccggtcg ttccagttgg
    15181 cgttcccgcc ggcgagacgg agataggcct cgtgcacgat cgccgtcgtg ttgagggtgt
    15241 ggcccgggcg ctccttgacc aggtggaaat gcgcgatccg gcgcaggtcc gggtagatca
    15301 ctcgcaccag ctcgtccatg gccccgtgct cggaccggcc gacggcctcg agcagcgccg
    15361 cgatgtcgag gctttcggtc atcaatcctc cgggtgcgta cctcgggatc cttatgcggc
    15421 ataactgccg cgacgcacaa ccctggagtg acgatcgtcg gcctcgaggc tggtgccggg
    15481 ccggcagaac tgcgcaagca gccggaagtt cgcgaccggc atgaccgcga ccgggatgga
    15541 agtactgacg gtgagcccgt gcgctgcccg cggttaccgg aagtgggaaa ccggtccgcc
    15601 tccccagcgc aagggtgccg ctgaggcgac ggctccagtc acgggacggg ttcgaagagg
    15661 tagacggcgc ccgagccgat cgctccattg tcggactggt cgccgtcgac gccggtggcg
    15721 gtgctcgcct cctgctcggc gcccacggca aaccttccct gcgagatgcc gacgctgctg
    15781 ccaaagaagt cggacgactc cgcattcgac gccttcacgt agtgctgctg cgtccactgg
    15841 cccgccgaaa tcgcgaagag gtaggccgcg ccggcgcccg gcgcactctc gtcggcctga
    15901 tccccatcca ggccggttgc ggcactgcct tcgtttctcg cggccgcgat catctgctcg
    15961 ccgtcgaaat cgagactggc gccgaaccag tcgtcgatgc ccggattgga cgccttgatg
    16021 tacgcggtct ggaaccaggc cccggaggtc ctggcaaatg catacacggc gccggagtcg
    16081 cgcagcgcat tgttcgcctg gtcaccgtcg atgcccgtgg cggcgctgtc ctcgcctcgc
    16141 gctccaacga gcaggatgtc gccggacaac gccaggccgt tggatccgag gttgccgccg
    16201 ccgaactggt cgcccgtcgc ggtgttcgac gccttgatgt aggcctgctg gccccaggcc
    16261 cctcctccat cgcgcgtgaa tacatagacg gcgccggcgc cgctggctga gttgtccgac
    16321 tgatcaccgt cgatgccggt cgcggcactc gcttcccgtt ccgcactgac ggccgcgctg
    16381 tcgccgtgca ccgcaacgct tcggccgaac aggtcgccgg cctcggcgtt ggacgccttc
    16441 aggtatgcct gttgagtcca gacgtcgcct gcgcgcgtga acacgtaagc agcgcctgca
    16501 tcctcggcgc tgtcgtcggc ttcgtcgcca tcgacgccga ccgcggcgct gtcctcgtgg
    16561 gtcgcggtca cgaccagcgt gtcaccgtgc agggcgacgt tctgcccgaa gcggtcgccc
    16621 gcatcggtgt tcgatgcctt gaggtaggcc tgttgcgtcc aggttccggc gctccgcgtg
    16681 aacacgtaga cggcgcccgc gtcgattgcg gcatcgtctg ccgggtcacc cgcggtcccg
    16741 gtcgaggcgc tgtcctcgcc cggcgccccc acgaccagtg tgtcgccgtc gagcgccaga
    16801 ctggctccga aaaagtcgag ggcagcggcg ttcgaggcct tcaggtaggc ctcgggttcc
    16861 cagcttccgt cgctgcgccg gacgaataca tagacggcgc cggcgtttgc cgccgagtta
    16921 tcgaacgggt ccggatgagc cgggctgatc ccggtggcag cgctgtcgtc ccactcgacg
    16981 ccgaccgcca gggtatcggc gtcgagggca agtcgtcgtc cgaagaagtc ctgcgtggtg
    17041 gcagccttca ggtacgcggc ctgtgcgacg tccgcgtcga cagcggcgag ccgtgtcacc
    17101 gcaacggaat agctgcgcgt ggtctggccg tcctccgccg tgacgacgac ctggaaggag
    17161 ttgagtcctt cggcaagcgc gattggcgca ctgctttcac ccgagacgac cgccgcgccg
    17221 tcgaccgtca cggtcgcgcc cggatcggcg gtgaccggcg tgagcgtcac actggcggtg
    17281 gcgaacccga cggtggctgc gtagtcggcg accgcggcat cgaacgccgg gtcgagattg
    17341 cccgacgaaa gcaccaggct ggccaggtcg gcattattgc tgagggcggc cggctcgcgc
    17401 gtgagggtga tcgtgtagtt ccttgtcgtt gcgttgtccg cggcggtgac ctggaccgtg
    17461 acgctcgtat tgccgacggc gagcggaacc gcggcgctgc cggtgccgcc gccgttcacg
    17521 ttcaccctgg cccccgcgtc cgccgccgtg gcggacaccg tcacggacgc cacgctgtac
    17581 tcgacagtga ccgtgtaagc cgtggttgcg gccgtgaaag ccggcgccag ctgtcctgcc
    17641 gacacggtga gcgtcgccag actggcatcc gcactcgggg ctggtgcagc cacattcccg
    17701 cggtggcggt cgttgtcgca ggccgacagc gccagtaggc agacgaccag actcgatcgg
    17761 gcaatggaat actgcatctt gctgactccg gctcccaggg cgtcgggcat ggcccgtacg
    17821 caggtaccgg accctcagca ggtcaaagcg tgatcggcgg accgaaccgc caggaaccct
    17881 gcagatttgc tggccgccgg cgccggcaca tgacgcgcgt gcaaccgcac gcggatcagg
    17941 tcgacgtacg ggaagccatg cgcagcgtgc tgcgttcgac gaggcattca gagtgctgga
    18001 ctcagcgctg ctgtgaccgc tgcgcggcgg aggcggtgcc cgggggcacg gtccccgcgg
    18061 cgctgccgag gatctggttg acctcgatga agtagccgtc cggatcgtac acggcactga
    18121 agagcacggg gatcgtgccc tggccgcccg gcgccgggta ctcgatgcgc tcgggggccg
    18181 tggcgaccgt gatgccaggc gtgtcgcgga tgcgcgcgaa ccgcgtctcg aggtccttcg
    18241 cgttgacgac caggatgact tcgcccgcct gcggcttcac gagcggcttc accggcggat
    18301 gcgtgacgtt caggcgctgc atcaggccga gcacgccgat gaacgtgtcg ttcgcgcgca
    18361 gcagcacgag acggatcgtc ggcggctgcg tccggccgtc ggggccggtg ccgcctccga
    18421 tctgctcgtc gtagaccact ttgagaccga gcgcgtcccg gtagaacgga agcgagcgtt
    18481 cgatgtcgcg tacgaggagc gtcgtgcggc gcacgtcgac gggcgtgcgc tcggcctgcg
    18541 ggacgggctc ggcgttcagc acggacggtc ccgacgcggc ggcgacgaga gcgacgacga
    18601 aggcgaggtg ggcgttcttc atagccgcca ttgtgcctca cgcccgcttg gcggaggcac
    18661 gcgccgttgc gagggggcgg caagcgcatt cggggaaata cttgggcgcc gtaccttccg
    18721 ggcggcgttc acgcagtgtc ccctctatac gccccgagcg tgccacaatc gcgcccatga
    18781 ccgaggtcgt cgcgcctcac ggcagcccgc tcggaatcgc cgtgctcggt gtcgaacgtt
    18841 tcgagacgag ccgcgagttc tactgcgatc tcatcgggtt cgacgccggc aagctcgaag
    18901 cctgggctgg gcccgatttc gagagccttt ggcacctgcc cgcgggctcg cgcgctcgcg
    18961 cgtgcctgct caccgcgagc ggctcggccg tcgggcgtat ccttctggtc gaattccagg
    19021 ccgcgcaccg caagcacgtg cgcggcagca tggactcccg cgcctacggc ctcgtgaatc
    19081 tcaacttcta cacgcgggac atacgcgccg cggcgcgctc cttcgccgcg ctcggatacg
    19141 agttgtggtc cgagcccacg cagtacgatc tcgcggcggc cgtcggcaag ccgatcgaag
    19201 tggtcttcga ggggccggac ggcgtcgccg tcaatctcgt cgagctcgcg agcaccgatc
    19261 caggcacgcg catcggccag atgcgcgcgt tcgtcgagcg ccatggctac acgcgcgcgg
    19321 gcttcacgcc ggtcgtcacc acctcgcacg tgtgccgctc catcgcgaag gctcgcgcgt
    19381 tctacgagca ggcgctgcgc atgggcgtgc tgatcgacga cgagttcaac tcgccggcgg
    19441 tgaatgcctt cctgcggctc aagcccggca gccggacgca catcacgttc atgcagggca
    19501 accacatgtt cggcaagatc gcgctgtccg agccgctcaa ctacgcgtgc ggcgatctca
    19561 cgccgctcgc cgtggcgccc aacatcggct acctcgcgca ggccttcgag gtgtcggacc
    19621 tcgcgcgcgc gatcgccggc gcggccggga cgcaagcccc catcgagctc gagccgcgcg
    19681 cgatggagct gcctggtctc ggccgccggt cagtcgccgt cgtgcgcaat ccgggtagcg
    19741 gcgcactgca gtggctcgtg gaatcgggct gagcccgatc gccgacagcg atctcccgac
    19801 gaggtgctcg gagtcgctca cgagcattgc tgaggaccat cgccggccac gataatgctg
    19861 gacgtcattc cagtctgtgg agaatcccca tgcccgtgta cctggtatcc gtcgttgaac
    19921 tcacgaatcc gggaccgaac atgaaacggt atgccgaaaa atcggctgag ctgattcgtc
    19981 agcacggggg gcgctacctg gtgcgcggca aggcgtcgga ggtggactgt ggggatctct
    20041 tcggcaatcg ggtactgatc gtcatcgagt ttccggcggt cgagaacttc caggcgttct
    20101 ggaacagtga tgcctaccag aaagactgcc ggcccctgcg cgagggtacg ggtatctacg
    20161 acatcgggct gttcgatgcc ccgccgcccg ggatggcctg acgttcctcg cccgtctccg
    20221 ggccggtgct gtgctgaggt ccgcccgccg ctatccccag gtaactcgtg acaggctgcc
    20281 gcggaaggcc ttgagcccgt aggcgatcag cgccgtgccc agcgagccca tcaccagcgt
    20341 caccagtgcc atggagtagt ggatcttcat ggggtcaccg aacacattgt cggtgaccca
    20401 gcccatggcg aaggggccga cgccggcgcc gccgatgccg atgacaagaa cgtaaatggc
    20461 agtgacgatt ccgcgcagct ggttgggtgt catctcggcg atggcggcca gcgcctgtgc
    20521 cggcgggtag ttggcgaaga tgccggcaat gcaatagccg gccagggcga gttcggggct
    20581 gggcatcagc ggcgccacac ccgtgccgat ggtcaggccc aggccaccga tgaggaccgt
    20641 gcgcatggtg ccgtcggtga agcccttcgc cttcagccag ttggtgacga agccgctgct
    20701 gatggcagag agaacgccgg tgagcccgcc gaagatgccg taccagatgc tgaacctggc
    20761 caccggccac tcgtggatgc gcacgaagaa tgccggcatc cagttcaccc agccgtagac
    20821 cgccatgatg atgaatgcca cgccgaagtg atggcacaac agcgtggtgc ggttctggcg
    20881 caggaaggcg cggatgtccg cccagctggc tttctggctc gtgccggctg cgaggtcgcg
    20941 ccgggcgggc tcccgcacgg tcagcgccat gagcagcgcc ggcaccaggc cggccatacc
    21001 gataatgatg aacacagcct gccaggcggg gacttcaccc agcacgggca tattgaaggt
    21061 gttgccgccg gtacccaccc cgacgaaggc cagcaccagg ccgccaccca ggtaggtgac
    21121 gccggtgccc agcgccgtgg acatggtgta gatcccgaag gccttcggac ggctcttcgg
    21181 ttcgaaatag tcgccgatga gcgaggtggt gcacgggttg agcgtggctt caccggcgcc
    21241 cacgccggcg cgcataaaga agagtcccca gaagctgcgc accaggccgg cacctgcggt
    21301 ggccaggctc cagatggccg taccggcggt cagcaggcgc ttgcggttgg tgcggtcggc
    21361 cgccaaaccg atgggcaggc cgaataccgt atagaacaag gcgaaggcca gaccctggat
    21421 gatgcccatc tgggagtcgg tcaggcccag atccgcctgg atagccggcg tcagcaggcc
    21481 gacgatgatc cggtccatga agccgaacag ataggcgaga caaatgacgc ccaggcagta
    21541 ccaggcgtag gcgggacggg gatagggctg gttctgggtc acgtgcgatt gttcctgttc
    21601 tcgtccgatt ccggagcggt gacgggcgtg gcgacactat cggataaaca ccctggtggg
    21661 aaatgcgggc ttcgcgcaga gcgccgacag gtggcgcgtc gccattcgac ggagtgcgaa
    21721 cttggcagcg agagccgtta cctgagcggc ctgcggctgg cctgaatccc cggggcgcca
    21781 ctaccgtccc aacgctggcc acgacccggt tcgacaggat cacggcgaaa ccttgcgctt
    21841 gatcgccatc gccagcggct gcggcagtct ggccagcgaa ccgagcacgt gcggcagcag
    21901 gcgcagtttc agccccgaga atgtgctcgg caccaccgct tcgatcaggt gcggtcccgg
    21961 tgcgcgcagc gcgtagccga gcgcatcgac cagttcgtcg gtggtggtgg cgcgcacggc
    22021 atgcacgccc attccggcgc cgatctgcac gaagtccagg ccggggttgc ccagctccag
    22081 ctgcgcgcgc gccttttccc ccgcgccaat accgccgccg gtcgcaccca tgcgctgcag
    22141 ttccatgttg aggatcgcgt aggagcggtt gttgaagaca agggtggtga cgttcagctt
    22201 ctcgcgcgcc atcgtccaca gcgcctggat cgtgtacatc gccgagccgt cgcccaccag
    22261 cgccagcacc ggccggtccg ggcaggcgat cgcgatgcct accgcgttgg gcagaccctg
    22321 gccgatggcg ccgccggtca gggtgatgac atcgtggcgc gccgcgccgc gggtgtaatg
    22381 cggcaacatc actcccgagg tctgggcttc gtcgacgatg atggcgcgct cgggcaacag
    22441 gtggcccacg gccttgcaca ccttctcggc ggtgagcttg cccctgggcc gctcggtgcg
    22501 gaccggtgcc tgcaggaccg gcgcagccgc gtcgacgccg aggcgggccg cgagtcgctg
    22561 caggccgccg agcgcatctt cggtgggcgt ggccagcgca tgcaccgtgc agccttcggg
    22621 gaccaggtcg ctcgccttgc ccggataggc gaagaacgac actggcgact tggcatcgac
    22681 caggaccaga tgctcgaggc catgcagctg caccgaggcc agttcggcca ggtaggccag
    22741 gcgttcgacg tgcggcaggc cggcgccgcg ctcgatccgg gtcgggaata cttcggcgaa
    22801 cagtcccgcg cccgtgtgcg cggcgatccg cgaggccgcc agcaggcccg gttcgcgcag
    22861 cgccctcgcg cccagcaaca gcgcggtctt gccgccgctg cggatggcct ggacgatggc
    22921 gtcgatggtt gcctcgtcgg tcacgggcgg cggggccacc ggcagccgct gcgcgggctc
    22981 gccaccctcg ctccaggaca cgtcggccgg caggaccagc gtggccacct ggcccggtgg
    23041 ccccatcgcc gccgccaccg cctgcgccgt gtccacgccc agcgcccggg tggaggcgga
    23101 ggtccgcacc caggtggaga cgttgcgcgc cacagtttct atatcggact gcagctgcgc
    23161 gtcgtacttg acgtggctgg tggcgtggtc gccgacgatg ttgacgaccg gcacccggcc
    23221 cttgcgcgcg ttgtgcaggt tggcgaggcc gttgcccagg ccacacccga gatgcagcaa
    23281 cgtggcggcc ggcttgcccg tcatccgcgc gtagccgtcc gcggcgccag tggcgacgcc
    23341 ctcgaacagg gtcagcaccg cgcgcatgcg tggctcgtgg tccagcgcgg ccacgaagtg
    23401 catctcggac gtgccggggt tgctgaagca cacgctcacg ccggcatcgg cgagggtttt
    23461 catcagggcc tgggcgccgt tcatctcatc gcctcctcgt ggtcctcgaa gccggatggc
    23521 tccagttggc cggcaccgat atccgccaag cggcgcacgc cggccagggt catggccagt
    23581 cgcagttcct gctgccagcg cgcgagcatc ccggccaatg cacgctcgcc gcccgcggcg
    23641 atcgcccacg cccagggacg gccgacgagc acgccgtgcg caccaagcgc cagcgcccgc
    23701 agcacgtcca cgccgctgcg gatgccgcca tcgaccagca cttcgacctg cgcgcccagc
    23761 gcctcgacga tggccggcag tctggccgcg gtcgcggcca caccgtccag ttgtcgcccg
    23821 ccgtggttgg agaccaccag cccctgcgca ccggcgcttg ccgccgcgcg cgcgtcgtct
    23881 tcgtcgagga tgcccttgag cagcaggacg ccgggccatt gcccgcgcag ccagtcgatg
    23941 tcggcccagg tgactgacgg gtcgaactgg gtgtccaccc aggccttgaa cgcgtccagg
    24001 tcgcgcgcgg cgggcagctg cccggcgagg ctgccgaaca cgtgcggctt gccgcgcacg
    24061 cccacgtccc aggcccagcc gggatgcgac agcaggtccg cgatccggag catccgggca
    24121 cggaacgtgc gcgcgcccaa gccatggcgg atatcgcggt ggcgtatgcc gggcaagggc
    24181 aggtccaccg tgaacaccag ggtgcggcaa cccgcatccc aggcacgttg cagcagcgcc
    24241 cgcacgatgc cgcggtcgcg cagcatgtag agctggaacc agaccggttt cgccacggcg
    24301 cgctggatct cgtcgagcgg gcagatgccc acggtggaca ggatgaaggg cacgcccgcg
    24361 gcctcggcgg cgcgcgcggc ctgcacctcg ccgcggcgcg cgatcatccc ggccagcccc
    24421 agcggggcca gcgccagcgg catggcgcag gcctgcccgg ccagcgtggc actcgtgtcc
    24481 acgccgtcga cgtcgaccag cacgcgctgg cgcaacctta gcctggccca ggcggcctcg
    24541 ttggcgcgca gggtctgctc gtcgccggcg cctccgtcca catagtcgaa caggaagcgc
    24601 ggcagccgca ctctggccag gcgccggtag tcctgcgcgg tggcggggaa tctcagggcc
    24661 atcagccacc ctccctgatc gaatccgccg ccgcgcgcgc ggtcaggatg cagcccggca
    24721 ggaacgtgcc ttccagcgag cgcttgccgt tggcgccgcc gccgccgaag ccggccgctt
    24781 cgccgacgca gtacaggccg ggcactgcac tgccatcggc gcgcagcacg cggctttgca
    24841 gatcggtctg caggccgccg agactcttgc gggtgatgag ccgggtctgg atcgcgatgt
    24901 agggcccggc gccctttgcc tgcagcggcg ccggcctgca ggtgcgcaac cggtcggggc
    24961 cccactgtcg cgcctgcagg atgcggcgga cctgctcgtc gccatccggc gaggagccat
    25021 ggccgaagtg ggcgtcgaag gcgtcggcgg tcgcctgcag cgtcgccgga tcgatgtcgt
    25081 cggtgccggt caggacattc atcttcgccg ccaatccttc cagactgtcg tcgaccagga
    25141 agtgccggct ctcgcgctgc atccgttcga gcaggcaatt gttgccgagc accgtttcca
    25201 gcacgaagcg caggaactgc ctgtcgcgga tgcgcgggtt gtactcggcg ccggaaatgg
    25261 cgaactcccg cgccgcgatg cgccggttca gaaggtgcca ggtccaggcg ccgtccagcg
    25321 cggccacgcg ccggcacagg tcgcgggtat cgaagccggt gaccagcggt gcgggaccga
    25381 tccgccggcc gcggtggtcc agccacagcg ccgacttgca gggaatcagc gaaaggccgt
    25441 gaccgggaaa gtgcggctgc gggtgcggga tgccggcggc gtagttccac atctcgccga
    25501 cgtgggtcaa ccggcctccg agttcgctgg cgacgcaatg gtgcagcctg ccgtcggcaa
    25561 agggatgagc gccgttcagc atctcggccg gtcgcgggcg gtcggccggc cagttcgcga
    25621 tcgcctgctc gagcgagccg ttgatgccgc ccatggccag cgccaccact ggcgcttcga
    25681 tgcgcagcac ttcgccggtg gcctcccgct cggcctgcac ccccgagatc ctgccgcctg
    25741 cgctgtcgat cgatacgacg cgatgcccgc tgagcacagt caggcgcccg ccgccgccga
    25801 ccgcattcat gccctggatc atgcgccgga ccagttcgcg cgaagtgccc cacacgatgt
    25861 ggtagcgcgg cacgctgttg ccttcgcccg cgtacccgcg ttccacccag gtgacggtcg
    25921 ggaagaactt gatcccctgc gccagcagcc agtcgtacac ccgtgcgcgc gaatgctcta
    25981 cgtagagttc agcccagcgc cgcggccaca tgtccgcctc gtccagctcg ccgaagcgca
    26041 tccagtcgcg caacgcgcgc tgcggcgaat cggcgatctt cctgcgccgc tgcagcggcg
    26101 tgtcgatcag ggccatgccg ccgaaggccc acagcgccag gccgcccaga cgcgcgggcg
    26161 tgtcacgatc gaccacggtg acccgcaggc cgccgcgcag gcattccagc gcggtcacca
    26221 ggccggccag tccgccgcca gctaccaata cgtcggtgcg gcgcgcttgc gccatgtgcg
    26281 tccgtctccc ccgtggcccg atccttgggc tggagcttac ccgacccgga ctgcgactcg
    26341 atcggcaacg gcgccgacac aggcggggac ggatgctcat gaatggcttg cagccctgca
    26401 gcgactacac tgcagtggat ggacacgcgc gccgacacgc cgagcgccga actgcgcccc
    26461 accctggaac gcctgcgtgc cgcatggcag gcgaacaagc cggactacgc ccagcgccgc
    26521 gacgatctgc gccgcctgcg cgacgcgctg aagcgacgat tggacgagat ggtgcgcacc
    26581 atcgccgccg acttcggcca ccgctcgccg gacgaaagcc gtatcgccga cggcatgacc
    26641 gtgctcaacg acatggacca tctcatccgg cacctgcgcg gctggatgaa gccgcgtcgc
    26701 gtcggcgtgg gctggcactt cctgccggcg cgcgcgcagc tgcgcccggt gccgctgggc
    26761 gtggtcggcg tgatcgcccc gtggaactac ccggtcaacc tggcactggt cccgctcgcg
    26821 accgcggttg ccgctggcaa ccacgcctac ctgaagccgt ccgaacacac accgcgcacc
    26881 agcgcgttcc tgcgcgacct gctggccgag gtgttcccgg ccgaccgcgt cgccgtcgcc
    26941 accggcggcg cggaggtcgg cgcggcgttc gcggcgctgc cgttcgacca cctggtgttc
    27001 accggctcca ccgcggtggg ccgcaaggtg atggccgccg ccgcgcacaa cctgacgccg
    27061 ctgacgctgg aactgggggg caagtcgccg gcgctggtct gcgacgacta ccccatcgac
    27121 aatgccgccg cgcgcatcgc caccggcaag tggttcaact gcgggcagac ctgcatcgcc
    27181 cccgactacg tgctggtccc ggccggcagg cgcgacgcgc tggtcgaggc gctgcgccgc
    27241 gaagtgcggg tgcgctacgg cgcggacttc gccaacctcg gcgactacac ccgcgtcatc
    27301 aacgacggcc agtacgcgcg cctgcagggt tatctcgacg acgcgaggcg gcgcggcatc
    27361 gacatcgtca ccctgggcgg cagcgccgac ccggcgcagc gcctgctgcc accgaccgtg
    27421 ctgctggagc cgggcgacga cgcccaagtg atgcaggacg aggtcttcgg cccgctgctg
    27481 ccggtcaaga gttaccgaac gctggacgag gccatcgcct acatcaacgt gcgcgaccgc
    27541 ccgctggccc tatacccgtt cagccacgac cgcgcgcgca tcgaaaaaat cctgcacgac
    27601 accatcgccg gcggcgtctc ggtcaacgat accctgctcc atttcgccgt cagcggcctg
    27661 ccgttcggcg gtgtcggcgc gagcggcatg ggcgcctacc acggccgcgc cggcttcgac
    27721 gcgatgagca agctgcagcc ggtgctgtgg caggcgcggc gcaccggcgg cgacctgctc
    27781 aagccgccgt attcgaaggc gaaatggctg atcgacctga tcgtgcggtg agcgcccgcg
    27841 cccgacatcg gcaggccgcg gtggtgagca gcgagcgata tctcatggcg ctcggcggcg
    27901 agaccatcct gctgcgccgg gtccgctact tcccccatca cctcctcggg cagactgccg
    27961 gcgccagcta agatgcgctg gcaagggagc tggcgagggc acgcagcagc gcggcgccgt
    28021 cgccacgcca cgcgctgccg tgcatgcagg cgaggacctg gggctgctcg cgggcgaggc
    28081 gctcgagtgt cgctccggtc tgcggggcat gcgcgtagta gtccatcgcc cggcggaacg
    28141 cctcgctcgg actgaggatg tcgccgtcgg tgaccgccac gctgccgtcg ccgccctgag
    28201 tgaagaggtc gccgcagagc aacgtgcggg tctccatctc catcatgagt ccgcactccc
    28261 agccgtgcgg cacatgcggc gtgtcgagcc atttgaaggc gtgtcggccg gtgcgaagga
    28321 tctcgccatc agcgagcgcg cgcgccgggc gcgtggcgat gtcgtcgacc gacaccatcg
    28381 cggcgacctg gccgcaaacg gcagcggctg cgggggcggc agcgaggaat tcgttcagcg
    28441 agccgcactc gtcggcttcg tagtgcgaga aggcaacgta gcgcagccgc tcgagcggca
    28501 cgactttgcc tatcgcttcg gcgacgagcg gaaacatccg gcgcgggccg gtgtggaaca
    28561 ggaggggctc ttcgtcgacg acgaggtact gattgaagtt gaagcccaag ccgcccgcga
    28621 tctcgaccgg tgtattgatg cggtaaatgc cggaggcgat ctcgtgaagg ttggtgccgc
    28681 tggcggtgtt cgtgatcatg ggtgtccctt ggttggctgt gatgcgtgcg ggtaacgccg
    28741 gggatgtgta acgaggcgcc gggtcgttac acgtcccgag aaatggttgc tatgcttggt
    28801 tcgtgccccg cagtctactt ctcgtcgaac ttgacgacgg cgcgctcgcc cggcgtattt
    28861 ccgagaccgg cgcagcacct gattcggcgg cggaagcaga actctatcgg cggctcgcac
    28921 cgcgcgtgcg gctctatggc ctgcgccacc tgcgcgacct gcaagcggcg gcggacctcg
    28981 tacagcaggt gctcctcatg acgctcgagc gtctgcgtgc cgggaaggtg cgcgacccgg
    29041 agagcattgc ctcgttcgtc ctcgggagct gccgtatgac ggtgctggag atgcggcggg
    29101 gaacacggcg ccgcgaggca ttgctcgaaa cctggagcga cgcggcagag ccctgtgagg
    29161 cgccggagca ggaggccctc gatccgcatc gcctcgccgg ttgcctggaa gccctgtccg
    29221 agcgcgaacg ctcggtggtg gtgctgacct tcttcgccga caggcaggca gacgaggtcg
    29281 catcggagct ggcgatcagc ggcgggaacg tgcgcgtgat tcgacatcgc gcactcgggc
    29341 gcttgcgcaa gtgcatgggc ggcggggagg aagtacagtg acttgcagga gccccgttgc
    29401 gctcgacgcg ctcgtcgcct actggctggg cgagctaccg caagaacgtg aagcggcact
    29461 ggaggagcat ctctttggtt gcacgcagtg caccgggcag ctcgaagagc tcgccgcact
    29521 cgccacgggc gtgcgcgcca ccgtcctcga cggcaaggtc agcatggtcg tgtccgagcc
    29581 gttcgtcgac gcgatgaagg acgcgggcct gagcgttcgc gaatatcagg tggagcccgg
    29641 cggcagcgtc aactgcacga tcggcacgaa cgatgacggc gtgatatcac gcatccgcgc
    29701 ccccctcgcc ggcgtgcaac gcctcgacgt cgtgcgcatg ctcggcgcca gcggcccgga
    29761 agagcgcctc accgacgtgc ccttcgatgc cgattcgggc gggctggtga tcgtcccgtc
    29821 ggcggcatgg ctgaaagcga tgccggcgtt caccatgcgg atgcggctga tcgccgtgaa
    29881 cgacgcgggc gaaaagcagc tcgcggagta cacgttcaac cactcgcctt cctgacagca
    29941 cgggtcaggc gcaaccctga tttccagatt tcgccctgct tcgatcacac cgggaccatc
    30001 aagctgtgcc ctcaaatggc gtgtgacggc ggttgtcgtg actcgcggca tcgcgcgcat
    30061 gctgctggag gcagtcggca tcgagcgcaa ggcgagacag cctgcaaatg tcgatccgct
    30121 tcagttagac agaagcgcgt gctcgacgcg atagcggcgc tcgcgataga tccaggccgg
    30181 catcagcaca ctggccagcg catcgcgatg tcgctccggg tcgttgtgct cgatcggagt
    30241 cagctgccga cccgcatcgc ccacactgaa gtcgcgcgcc gtaccattcg gcagcagcac
    30301 ggtcaggacg ttgccttgaa gacgcgcgta ggactgctcg tactgcatca tcgctcgtgg
    30361 cggtggaccc gatgagttgc cgaactccgg cagggttcgt gtcaggtcgc ggcccggcat
    30421 cgggtgcgtg ttgtccacgc ccatcagcga cagcagcgtg ggtgccaggt cgatctggct
    30481 gacaatggac tggactttca gcggtgccac gtcggcaccg aggatcacgc ccgggatgtg
    30541 gaacttgtcg agcggcacca acgcgctgcc gtgcacgcgc gtgtcgtgat ccgcgaccac
    30601 gaggaacagc gtgtcgcgcc agtagtcgct gctgcgtgcc tggtcgagga actgcccgag
    30661 ggcatagtcg gcatacttca ccgcgttgtt gacggtgtgt ttctctgcat cgaactgttc
    30721 gatgcgaccg tcgggatact cgaacggcgt gtggttcgaa gaggaaaata cgaggagaaa
    30781 gaagctctgc tgctggccgt gcagcgcctc gatgcgctcg tgtgccttcg cgaacagatc
    30841 ctcgtcgctc gcgccccagc tgcccacgaa ttgcggatca ttgaactggc tgcggtccac
    30901 gacggcgttg aagccgttgc cgaggaagaa gccgcgcatg ttgtcgaagt gcgactcgcc
    30961 gccgtagacg aactcgctgc gaaagccgct gcgcctgagc agcgatgcca gcgtcgtaaa
    31021 gtcgcgctgg gcattgggca acttcacgac gctgagcgcg ggcgtcggag gaaacccggc
    31081 gacgacggcc tcaatgccgc gaatcgagcg cgtgcccgtc gcatataagc gttcgaacca
    31141 gataccctgg tcagcaaggc ggttcagggt tggcgtcaca ggcaatccgc caaggcgctc
    31201 tacgaagccg gccccgagac tctcctcgag cacgatgacc agattgagcg gcctggttcg
    31261 ttgcacggag ggctgctggc gatgcagtgt gggcagctga tcggaagtga agcgcgcggg
    31321 cggcacgccc atgtccgcac gcaccctgcg cacgatcacc tcatgcggca tgctcccgta
    31381 gctggtgccg gtctgatcct ccgcccgcat cgcgagtgat gccgacagta cggtataggc
    31441 cgagttggcc accaggctgt tgaccatcgc gtcgtcgcaa aacaggaagg aggcgagatt
    31501 ggccggccgg tgctccaggc tggagcgaat catcagtacg gcgaggatga ccatcactgg
    31561 ccagagcaac gcagcgcgca gcgctcgtaa gtgaattgcg ggtgcttcgg ccacgcgaaa
    31621 gtagcggatg ctccaccagg ttgctccacc gagcaccagc aacgtcgtga acaacgtgcc
    31681 ccggtacccc tgccacagca tggccgacac ttcgccggga taagcgaggt actgcaacag
    31741 catcgtgccg ggtcggccgt cgtactcgat caggaactgc ggtgtggcga gctcgagaaa
    31801 cgagatcacg atgaagcaga gtgcgaacca tgccttgcag agttgctccc accacgccat
    31861 gcgcttcatc atcagcagca tgggcagcgc cagtagcgca ggcgcgatgc acacaccgat
    31921 tgtgatcaaa tcgctgcgca ccccttgcgc aagcatccag gcgaccgcat cggtctctgc
    31981 aacccgccgc cactgccacg ccatcaacag cagccgcgaa gcgctcaaca gcagcagcac
    32041 gggcagcatg aagcgaagca tcagtccata gggcccgagc caggcttgga ctgcgcgcca
    32101 gtggccaata gaaaccgaat ctcgggaaag gccatgcgca acaatgctcg attcggaggc
    32161 catgggcgtt ggaagtcctg ctgtcgagtc aatttccggc cccatggttc gcgattccct
    32221 gatgcgtgga accgcctgct ggctgagagg cgcggtaagg cctgacagtc accgctgctg
    32281 ggtacgggct c
//
LOCUS       DAOQVY010000012        31075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_1707_length_31075_cov_6.195132, whole genome shotgun sequence.
ACCESSION   DAOQVY010000012 DAOQVY010000000
VERSION     DAOQVY010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 31075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31075
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1707_length_31075_cov_6.195132"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PKN91_02245"
     CDS             complement(<1..676)
                     /locus_tag="PKN91_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26920.1"
                     /translation="MSAPRGEPLQMFRSGNVLAAEQACMLRLETHPDDVEALKVVAVA
                     ALRRGDAVRAIAKLTHASRVDPADTIVRYHLARAHEMGGNIIASLAWYDSALALAPGF
                     HLARLNYGAALERAGQGGRALWQWARALRDAQAQGRWLDAATTPSGLQPLVERAARAV
                     RNGRRAMLAQTLEPLAAQYGRAALERVHRALRIYLREERAQPDDPRQQPTFLAFPGLP
                     AAPILDR"
     gene            709..1293
                     /locus_tag="PKN91_02250"
     CDS             709..1293
                     /locus_tag="PKN91_02250"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HNS26921.1"
                     /translation="MALADLRHYIRTYDDSLDAALCARMIASFHGLERFHAENGANVR
                     RGLEESRWTELNVSRYADAAFLGMFRRTIEAALARYNADVGLGIAVPDSGTNSDLVMK
                     RYRPGGGEKFQLHFDAVNHVANRYLVMLWYLNDVAEGGETRFPQLDFAIAPRAGRLLV
                     FPPYWMYQHEGVPPASGDKYIVSTYLLFTAHPAS"
     gene            complement(1278..2978)
                     /locus_tag="PKN91_02255"
     CDS             complement(1278..2978)
                     /locus_tag="PKN91_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNS26922.1"
                     /translation="MVGAAAHAGGRYDTAESVFRHLTELQPTRAAHWLNLGTALRGLA
                     RYDDALRAYARAHALGGFSPAFCYNVGLTHLDRLDYESARAVLARGVSLAPQDAAMRL
                     QYANACFESGHTEEGVRALADEQLLAALPPAQVADAGQRLQGAGESALAAAAMSRLMA
                     LPDLELQSGLTLVHMLERANRLADAGAVLAALRHRHGAAPTAALSSAEGRIAQRAGRH
                     AEAIAHFERALALETGAHRRHYDLFPLARSLDAAGRTDEAWERLGEAHRSQLRQVSLA
                     APLHALRGAPTFAIADRGSERQDVAAWDTRGAPAAADSPVFIVAFPRSGTTLLEQVLD
                     AHPHLVSMDEQPCLQHALEELAAAGGCYPDRLAVIAPADLEAIRRRYFERARQKCALA
                     PGQRLVDKNPLNLLRLPAIRRLFPHARVILAIRHPCDVLLSCHMQHFTAPEFALLCAD
                     LPRLAAAFRKAFDFWYREQATLQANVHELRYEGLVTDFEREVRALAAFLDLPWHDALL
                     APAEHALGKAFISTPSYSQVVQPVNARAVGRWQAYRRHFEPALPLLAPLLERWGYDAG
                     "
     gene            complement(3014..5884)
                     /locus_tag="PKN91_02260"
     CDS             complement(3014..5884)
                     /locus_tag="PKN91_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS26923.1"
                     /translation="MISPETNPLVRHAVRLSMMAGAGAAAAVALPTAAQAQDQATTDT
                     DDIDTVIVTGSRIRRLDTETASPVLTISAEDISRTGLTTMGDVLQRLPTVAGAATNPA
                     VNNGGGTGESNVELRGLGAQRTLVLVDGRRVGVYGNTQTSAVDINMLPINMIERVEVL
                     KEGAGAIYGSDAIAGVVNFITRRDFDGAEIGAQYGQTGESDGERTSVSLTVGNQTDQL
                     GIVFGANYNRQEKVSAGDRAFSATALYLYGGVVTAGGSSRTPTGFIQVDAATQAQFGC
                     DNPFLTRTDGAPGTSLADFRCFDIPGDLYNYQPFNLLMTPQERGSLFTNVNYEINDMA
                     EAYGQVVYNQTRSGFAIASLPFDAVADDTIISADSIYNPFGTDFGGGAGDNPAMRARL
                     ESLGNRRSKVRSDQLFANAGLRGEFASTWQWDVNAGIGHMGQSTNISGYLLKDKLNQA
                     LGPSFVDADGEHCGTPGAIIAGCTPINIFNLEDPAQIAALNGISTNYRTDYTFRSSQF
                     SAGLNGDLFEMPAGAIKAAVGAEYREQEGVFETDALTRGEAPLFLTCLLAQETCTGNG
                     AADYDVRELFGELFVPLLADLPGVQALNLTAGVRYSDYSKDTIGDDTTFQAKLEYRPV
                     RDLLLRASYAEVFRAPTIVDLSLAPTQTAATFNDPCAGLTSAQLAANPNLALACVGVA
                     PDTGFQQPNSQITGLLTGNDLLKPETGDVLTAGFVYDSSLIRGLSFNVDYWRYKIDDI
                     ITAIDPNFAADQCVQNGDTDFCDLMFRFESGPSAGQFQVFQLPTVNLGTLETDGVDVG
                     VRYALRDTPIGSWTFQLEATRINSFTSVPVPGAPPVEVAGTYDPQYGNYAKWRGVAGV
                     GWTFGGFDGALTARYFDSLTIKDADGRIPDQALDIPSVTYLDLTLGYSFASNTRLQAG
                     VQNLTDEEPPFFYQNNVTNANTDVSTYDVLGRQWYLGVTQKF"
     gene            complement(6032..6931)
                     /locus_tag="PKN91_02265"
     CDS             complement(6032..6931)
                     /locus_tag="PKN91_02265"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HNS26924.1"
                     /translation="MKRNAAAAFALAGALSTPQLLGATELAPVADESPVFATPTTFDQ
                     VGRILVPVMINGEGPFRLIVDTGANRTTITTEVATRLGIDYVSAPTVILNGVTGSAEV
                     PVVELTRVHAGELVLDDLQAPVVLPHLMGEADGILGVAGLREQRLFVDFRRDIVRIGR
                     SRFERGDDLRIRAERVKGGLLGAQVRVGRVPALAVFDTGAQKTLGNRALQAALSADDR
                     GRQTMVYGTTEATMTGDVAAVPDILADRVIFRDVVAAFGDFHVFDLWGLNDVPALIIG
                     MDVLGAMDTVVIDFRLAEIRIRG"
     gene            complement(6985..8775)
                     /locus_tag="PKN91_02270"
     CDS             complement(6985..8775)
                     /locus_tag="PKN91_02270"
                     /inference="COORDINATES: protein motif:HMM:NF025134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS26925.1"
                     /translation="MPEALRLLQGGQLAAAETTCRQVLSRHPRDPAAIHLLGLIRKDA
                     GDVAACERLMRESIAIEPRSAEFRANLASLLRRLGRMEEAEACYRDALALDPRNRAAR
                     LSLARTLVDLGRPGDAEAECRTLIASGTAEAAVWSLLATALRSQHRLEESEAAFRRAI
                     EIDPRQGAAHHNLGALLSQLERAGEALQALQQARQRGVSGFELEFNSGRALMQLTRLD
                     EAERAFEAAVALQPLHVEAQLNLARLRWLRGDADFARSVRAAAAAHPDDVAIQMLFGR
                     VLWRAGDLAGAEALLRDLLARRGSMPQARSMLAMVLFESGRPAEAQHEALEAARAQPG
                     EPTVDDPLVSILLARGLADEAMPYILGQRARQPNEQLWIAHEATAARLLGKALYEELY
                     DYDRVVRKFELETPKGWSSMQELNAAAIEALAKRHVAATHPLDQSLRHGTQTLASLLT
                     DPDPAIRALIKAFEGPIESYRALIGTDAAHPLSARNTAPARLAGAWSVRLQKEGFHVN
                     HVHPEGWISSAYYVSVPAEVADDAQMSGWIKFGEPRIPTPGATAERYIRPSAGSLVLF
                     PSYMWHGTNAIHGNDARITIAFDAVPARSG"
     gene            complement(8807..9568)
                     /locus_tag="PKN91_02275"
     CDS             complement(8807..9568)
                     /locus_tag="PKN91_02275"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HNS26926.1"
                     /translation="MADNAEPAVSGAAEPPFRLAVPVDPAVMASVYGRLGRVLMPGMF
                     DPETARALYKCLTEEIPWQLHFNGSEGVYDLTERQLAELDAEARAKLMAAVHTGARGG
                     FQFLYRNYPLSDEYAAGRGREYIVARAFEFLSSPTFLDFARKVTGVAAIDTIDAQATC
                     YGPGHFLTQHDDLAENKNRVAAYVIGMTPVWRTDWGGLLNFIARGGHVAEAYAPTFNT
                     LTIFRVPQAHSVSLVAPFAGYGRYSISGWLRTGQG"
     gene            complement(9640..12510)
                     /locus_tag="PKN91_02280"
     CDS             complement(9640..12510)
                     /locus_tag="PKN91_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS26927.1"
                     /translation="MSAVAAATALPAQVQAQDEATIDTVVVTGSRIARPDFESPSPTV
                     SVDSQVFAQTGTATVEQVINSLPQFVPSITNTSNNPSNGGQSNIDLRGLGSQRVLVLM
                     DGRRIVPSNGSGIVDLNILPSQLVETVEIVSGGASAVYGSDAISGVVNFRLKKDFQGL
                     ELDGTWGQTSESDGQEYFATLTAGTNFAEDRGHIMGSVSYTEREEVFAGARSHSRVAL
                     GYDDPGFSPNGSPTFEEGRVTVAASQAARDAVFGSYGLPAGIAQATYGFNSDGTLFTV
                     GDGTPGSVLNFKGDSAADTFNDANYTYNFAPANYLQLPLERTTAFAHAGFDVTESFSV
                     YGQVLYSDMTGEQFLAPTPLSTVYTPPTNPFVPADLATLLASRADPNAPFTLTRRMTE
                     AGGRVQTYNSEAYQVVLGFKGSIFDSWNYDVYGAIGNVDVDSPQSGNISREKWEELTF
                     APDGGVALCGGFNPFGAGAITQECVDYITVDTNPREEIDQTIFEATISGPVFDLPAGS
                     VNMAFGAFYKEDEYAFIPDDALARVIPATPYSGARFDIAGFNAALPISGKIDSTEFFV
                     EALVPVLKDMPGAKSLDVSLGYRYADMSGIGGVDSYKGELVWRPVEALSLRGSYQRAV
                     RQPSVTELFQPQVPNFPAYTPPDPCSINSPERLGANAAAVRQLCMDQGIGAGLIDIYS
                     FGGSQVQGLSGGNPLLTEETADTFTVGFVLRSPSDNAFLSGLSLTVDYWDIEIENAVG
                     SIPARTFVSRCYDPAYNPTFDVNNLFCQLVGPRNTSSGQIEVALEANSNIGAYQAAGY
                     DVQLDWVGDLGPGRARVNWLMTFLDKWDTQEVPGDAFTELAGTSGYYGAGGSFPEEKF
                     TLNLGYDWAGFDVLARLRYVGEMDDIAFPEFKLDATTYVDLSAGYRFKEGMLDGLSLR
                     AGVINLTDEDPVLFPSYQQSNTDPTVYDVLGQRYYFNVNYKF"
     gene            12899..14260
                     /locus_tag="PKN91_02285"
     CDS             12899..14260
                     /locus_tag="PKN91_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNS26928.1"
                     /translation="MPPRSAALTEGPIARSLIVFALPMLAGNVLQSLNGSVNAIWVGS
                     FLGEAALTATSNSNTVMFLLIGSMFGMSMAANILIAQHFGARRLAEARRVVGSSASFF
                     AAVAIAVAVLGVLFSDHILRAMHTPPDALPLAHDYLRIIFVAIPFMFMYAFAMAVLRG
                     AGDSRTPFLFLLLSVGLDILLNPLLIFGVGPLPRLGIAGSALATLIANIVSLGALVAH
                     LYRKRHPLVLHRDDLHLLRIDRGIIATLLGKGVPMGLQMVVVSASALLMLGLINQYGS
                     QTTAAFAACMQVWSYVQMPAFAMGAAVSAMAAQNVGAGHWGRVGRIAMAGVTFQTLVT
                     AVSVLLITVFARPLLGIFLPADSPSLATAVHVNYVASWSFVLFGVTIVLFGVVRSTGA
                     VIVPLVILVITLWGLRFPAAASLMGRYGEDAIWWSFPASSALSTMLAVAYYRYGGWHK
                     ARM"
     gene            complement(14271..15017)
                     /locus_tag="PKN91_02290"
     CDS             complement(14271..15017)
                     /locus_tag="PKN91_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26929.1"
                     /translation="MRTLLVAALALVLGILLARGWWQGAPAGDPVQVIVTQVRTHAII
                     EHERQIAIWYRSCPEVVGIDPEIFVAWPGKLSYELGLADVEITREGDVLTVKTGAIRA
                     DEPAIPTDFIDYLTTSPWFNFANEQELVNREVARASPIARYLSVYYQRRDSSLVEDFR
                     KELAELVQRLAGALGLTVSAVNVEIPEVDAKLPKLPTLELCESTTAAVNGLPFAKVDA
                     GYTTPIAFDAAAAAGRKPASGVIAGVGAKE"
     gene            complement(15021..17090)
                     /locus_tag="PKN91_02295"
     CDS             complement(15021..17090)
                     /locus_tag="PKN91_02295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNS26930.1"
                     /translation="MKRALILAVAFCCCAAVQAADLRPITHEDVWLAKRIGDPVPSPD
                     GRWVVFTVAEPAYADEDRLSDLWLVPADGSAPARRLTNSRGGESDVAWSEDSQRIVFS
                     AKRDEDKAAQIYILDLGFGGEARRLTAASTGARSPVLAPDGASLAFVSDVPDDPAAAA
                     EPKYNARVYDGFPIRSWDHWRKPQRPHLFVQPLTAAGNPAGEARDLLAGTKLAAAPGF
                     AGRGGNAGDNLDPAWTRDSRELVFVASENHNQAAFAFTDTQLYRVPAAGGAEPVRLTR
                     GPDRYRNPAFSPDGRRLFAAFERGTGKPYNIDQVVALDWPAVGAPLVLTAPLDRPATS
                     FAVSADGRTLWFLAGDSGRHKLYSTPTHRAAPRLAYELESGNYGSLALAAKAKSPVLV
                     ATWDSATHPPEVVRLEPRARRHTLLTGFNAALAAQIDWQPLREFTFENRRGDTIHSFM
                     ALPPGFDPAKKYPLLVLMHGGPHGMWTDQFVVRWNYHLLAQPGYVVLLTDYKGSTGYG
                     EAFAQSIQGDPLRGPADDINDAADEAIRRFAFIDASRQCAAGASYGGHLANWMQASTT
                     RYRCLVSHAGLLDLRSQWATSDVIYHREVNIGSTPWSGEALWQEQSPLTYADRFRTPV
                     LVTIGERDFRVPLNNTLEYWSVLQRQQVPSRLLVFPDENHWILAGENSRFFYAEVQAW
                     LAKYLKE"
     gene            complement(17141..18466)
                     /locus_tag="PKN91_02300"
     CDS             complement(17141..18466)
                     /locus_tag="PKN91_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS26931.1"
                     /translation="MRRSLEGTLLAVLALAVLLAAGSALLAVRWLTPWAAMLCGLLVG
                     ICASVWLARGVARRWTATAIALRDGIASLRDRDFSVSITRTSDDELGELVEAYNSLGD
                     LLRRERVDLYQRELLLDTVIQATPLALVLTNAAGTVIYSNVAARELFAGGHRLEGADF
                     AATLARAPGPLREALATAQDRLFTVELGGEPQVYHLSQRHFQLNTQPHRLLLLKRLTR
                     ELAAQEVVVWKKVIRVIAHELNNSLAPISSLAHSGQLLLEAPDTVPLRKVFSTIEERA
                     THLAGFIDGYARFAKLPKPRLAPLDWPALVSRLQAVAQFTLRGEPPHITVHADASQLE
                     QVAINLLKNALESGSAASDVELEVRARRGGVVLEVYDRGAGLSGEALTSALLPFFSTK
                     ASGTGLGLTICREIVDAHGGHLILANREGGGALASVWLPASGPLEERPR"
     gene            complement(18466..19383)
                     /locus_tag="PKN91_02305"
     CDS             complement(18466..19383)
                     /locus_tag="PKN91_02305"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HNS26932.1"
                     /translation="MESLQQGAEAGNAQAQYQLGARLLVGRGAPFAPPQGLKWVQAAA
                     SQRHPQALALLAVLTSLSGDWPEAFKLLRRAADAGEPLARVQAGILANPAQFDREQWN
                     LPLAPRWQSESPRIGIIERCIPRAFCDWIIGRARWKLDAARVQDPATGEVRQMDVRTN
                     SGTGFSLVESDLLLQMVNARVGAAIGVPLSHQEPTNVLHYAPGEEYRAHYDYFTQAEP
                     HVGDLQVAGQRTVTVLIYLNDDYEGGETDFPELGWRYKGGTGDALMFWNTTPEGEPDP
                     RTLHAGLPPTRGEKWLYSKWVRAKPYPLL"
     gene            complement(19385..20767)
                     /locus_tag="PKN91_02310"
     CDS             complement(19385..20767)
                     /locus_tag="PKN91_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNS26933.1"
                     /translation="MRQATRDALRDSLMNRTVLVIDDNEAVRTAFEVLLSIHGVRTLV
                     AGSPGEGLATLAREPVHLVIQDMNFQRHATSGEEGVRLFRAIRASRPELPVILLTAWT
                     HLETAVELVKEGAADYVAKPWEDARLLTSVRNLLDLQRAQADMQALRSERAAARSALA
                     ARHDLAGIVYASERLHVVVSMAAQVAQAGVPVLITGPNGAGKEGIADIVQANSAVRRG
                     PYVKVNIGALPDDLIEAELFGTEAGAFTGARARPGRFEAADGGTLLLDEIGNLSASGQ
                     AKLLRVLQTGEFERLGSNQTRRVRVRVIAATNTNLRQAIREGRFREDLYFRLNVIELE
                     VPSLAERRDDILPLARHFLDADLSLSPDAERALLRYGWPGNVRELRNTMQRAALLADG
                     PMITAAALGLPAVPGVPPAPEEATLDRDTIEQALARCSGVVAHAARDLGLSRQALYRR
                     MEKLGLKSTA"
     gene            complement(20811..22031)
                     /locus_tag="PKN91_02315"
     CDS             complement(20811..22031)
                     /locus_tag="PKN91_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS26934.1"
                     /translation="MVMSDIRPVLSALLRNRTGAILVSLQVAIALAVLVNAIYIVKQR
                     VDLIGRPTGMDVANVFTVISSGFTQDHDRLAAARNDLAWLRSQPGVVAATRTNAIPLS
                     GGGSSTGIRSEPDAPNDKTVAINYFEVDEGGLETFGVQLVAGRNFRAEEIMPPDPNRS
                     QAPPVAIITKSVARELFGTTDAVGKVAYDYMNRPTTIVGVIEHMHGSWVSWDRVDQVA
                     LLPMTPAFGGDNLYLVRAAPGARDELMRKAEEYLATSDPTRMVRGVRALEYYRDRSYL
                     SDRNMAIFLVSVTALLAGVTALGIFALATFNVNVRTRQIGTRRAIGARRADIVRYFMV
                     ENWLVTTGGVLLGVALAFGVGYWLSTQYELPRLDLYYLLGGVLLLWALGLAAAWQPSR
                     RAARISPSVATRTV"
     gene            complement(22047..23378)
                     /locus_tag="PKN91_02320"
     CDS             complement(22047..23378)
                     /locus_tag="PKN91_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS26935.1"
                     /translation="MFGYYVKLALRSLRRNPGISALMVMAIALGIAVCVMTLTVYHGM
                     SSNPIWWKNDRLYAITLDSWDAEEPANEERPELPPPQLTYRDAEAVTSTAIAARSVRM
                     MRTGGVLSSAEAASDVLPERALLRVTTAGFFGMFDVPFAHGGGWQAAADTGPEPVIVL
                     SKAMNEHFFAGANSVGRTLRWNDREFRVVGVLEDWHPSPKFYDLNNGSFDDPEDAYVP
                     FGWLPALELDSVGNTNCWKPEQRESYLDFLNSECVWVQAWVELPDAATRVRMQEYLDG
                     YVNEQRSSGRFARPDNNRLTPVDQWLADNDVVRNDNRMLVAMAFAFLSVCLINTVGLL
                     LAKFLGGATITGIRRALGASRRQIFLQLLTEAGMLAAVGATAGLVLASLGLLGLRAMY
                     ATGSSGFRTVMYIDVWSVAMAVALAVVSALAAGLYPAWRVGRLAPAVYLKS"
     gene            complement(23389..24105)
                     /locus_tag="PKN91_02325"
     CDS             complement(23389..24105)
                     /locus_tag="PKN91_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS26936.1"
                     /translation="MLAMRHISKVFRTELIETHALRDFSLDVASGEFVAVTGPSGSGK
                     TTFLNVAGLLETFDTGRYELDGVDVNGLSDDTRSRLRNEKIGFIFQSFNLIPDLDIFD
                     NVDVPLRYRRMKTAERKERIESALDIVGLSSRAHHMPSQLSGGQQQRVAIARAIAGDP
                     KFLLADEPTGNLDSLMARQVMDLLEQINEMGTTIVLVTHDPELARRAHRNVQVIDGQV
                     SDYKPYEPREPQVMASAAMA"
     gene            complement(24128..25459)
                     /locus_tag="PKN91_02330"
     CDS             complement(24128..25459)
                     /locus_tag="PKN91_02330"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNS26937.1"
                     /translation="MKISALRRPGDTSTEHPAAGAIRGTASQDVAREPSRPGWRRHRK
                     LQIAGGAAVLLLLALVWGVRGWFAAGNTVPLERLRIATVERGHFVRDVAAQGTVIAAV
                     SPTLFASAPGTISYAVHAGQVVARGDVIATLDSPALRNEYERERANLAGAEAALARES
                     IEVRRQNLLNQQQADLADVQIRAAERELARARAAWETRAIPERELRQAEDDVATARLN
                     FEHAQATASLERDSLELELRTRRLERDRQALVVENLRRRVDELTVRSPVDGTVANLAQ
                     TDRANVAENAPLVTVVDLGVFEVEFQVAETYATEIRPGMAAEITLDGRTHAGTVASIS
                     PEVRQSQVTGRLKFAGEDPPSLRQNQRASVRIVLDERNDVLAFDRGSQLGETSTSVYV
                     VRGERAVRVPVQLGGASISKIEALQGLEPGDRVIISDTRDFRGAPEVLITD"
     gene            complement(25527..25730)
                     /locus_tag="PKN91_02335"
     CDS             complement(25527..25730)
                     /locus_tag="PKN91_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26938.1"
                     /translation="MTIKFAGMRIIALIAAALITAIGMSPYEFLHYSGLSAAAAATVD
                     VRGRVRCPLPDMQTRPICIIAVK"
     gene            25841..26764
                     /locus_tag="PKN91_02340"
     CDS             25841..26764
                     /locus_tag="PKN91_02340"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNS26939.1"
                     /translation="MDAAYDRLPLNALRVFEAVATRLNFADAAEALHVTPAAVSQQVK
                     ALEDYLQTPLFRRRGRSIALTSEGADLLPRVRRGLDELEAALQQSRRQRESGVLNISM
                     LSSFLQKWFAPRLGAQHDHLHDIELRVHTSREAVDFARSDFHAAIRLGAGNYPGLYSE
                     KLLDDWLVPVATPALVRKYGMIPRAKDLSAFPLLYSSDEPWSSFVADATAGSAKTRPA
                     LLDDSVSVISAALEGHGFAVARWSLVQRDVAAGRLAIACDVVRQFRFSYYFVCPDSYA
                     AMPKVVRLRDWLRAEAARHPLPPTMALSLKP"
     gene            26900..27262
                     /locus_tag="PKN91_02345"
     CDS             26900..27262
                     /locus_tag="PKN91_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HNS26940.1"
                     /translation="MKRIIFGAGALAAMAFSAAVFADAGERVTVREVPVHYSDLNANS
                     SQGAQQLYSRLKSAARAACGNEPGRRMEERIAYDNCRMDALSKAVSEVNSRTLTALHR
                     SRIAPLELARFDNAYTAR"
     gene            complement(27379..27606)
                     /locus_tag="PKN91_02350"
     CDS             complement(27379..27606)
                     /locus_tag="PKN91_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HNS26941.1"
                     /translation="MSAKSPDALYELRARFEEYDADSDGRIEFAEFCQLMAELDDELS
                     REECQLAFQGADGDDDGSISFDEFAAWWTGG"
     gene            27669..27929
                     /locus_tag="PKN91_02355"
     CDS             27669..27929
                     /locus_tag="PKN91_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="involved in start site selection during the
                     initiation of translation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HNS26942.1"
                     /translation="MKGPGGKPVPVRVGRETKGRGGKGVTVISGLRLSVAELEAIAGE
                     LKRRCGCGGTVKDGHIEIQGDHRDTIVAALRARGFDAKRSGG"
     gene            complement(27965..29698)
                     /locus_tag="PKN91_02360"
     CDS             complement(27965..29698)
                     /locus_tag="PKN91_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HNS26943.1"
                     /translation="MVRQGTAWILVAVLAALSVGCSTRTDEMRAAAAAAPAAWTTFRD
                     TFIEDALRDNPPFAVDAGRHEYDGGLPDWSKAGIAARIARLHAARDAAQAIGPLGLDA
                     AQLFERDYLLARIDRSLFWEETAELPFTNPTYYLGLLGPSVYLTRDYAPLADRMRAYV
                     TYAKGVPGAAAQIKENLRLPMPLTFVERAISGFGGYASFFDTDVPAIFASVDDAALQA
                     EFATANKAAADAMRDLAKHFEAQRAGATQDFALGAERFAQMLAATEQVTTPLAELEKI
                     GRADLERNLASLQTACEKFTPGKSIKACFAKLAADKPKGGPVAGATRQLVELRQFVID
                     HKVVSVPSEEQALVAESPPYNRANSAYIEIPGPYEKSLPATYYIAPPDPKWSRADQLA
                     YIPGEADLLFTSVHEVWPGHFLNFLHANQSQSLVGRLFVGYAFAEGWAHYTEEMMVEM
                     GLHKDDPAAQVGQLSNALLRNARYLSAIGLHTGGMTVAESERMFLEQAFQDPGNARQQ
                     AARGTYDPAYLNYTLGKLMIRKLREDWTSTRGGRGAWKQFHDEFLSYGGPPIPMVRKA
                     MLGEDDEGALF"
     gene            29775..30545
                     /locus_tag="PKN91_02365"
     CDS             29775..30545
                     /locus_tag="PKN91_02365"
                     /inference="COORDINATES: protein motif:HMM:NF022665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HNS26944.1"
                     /translation="MRHRYVVGAVIAFAAISGLVYLFWPKPIAEPPVLPSTVTDAPLP
                     PPIEHPLPEAPPDSAAPPLPALADSDAYLLDALGELIGAQPANDLVVVPGIARRIVAT
                     VDHLGRDKLPVLTRAVGPIPGSLAVVRDGDRITLAEANFARYAPYVALVERLDVSRLA
                     ALYQRIYPLLQQAYGEIGPEGRYFNDRVIAVIDDLLATPQVAGPIELEQPSVHFRFKD
                     PALEARSSGQKLLLRMGEAHATVVKERLLTLRALIARQ"
     gene            complement(30539..>31075)
                     /locus_tag="PKN91_02370"
     CDS             complement(30539..>31075)
                     /locus_tag="PKN91_02370"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNS26945.1"
                     /translation="PNAPNLSMARCYAGTVMLEGTTLSEGRGTTRPLELFGAPDIDAS
                     AVLREMQRIAPGWLGGCRLRPCWFEPTFHKHVGKLCSALQVHVDDTAYLHEAFRPWRL
                     IAVAFKAIRGLYPEYPLWRDFAYEYERDRLAIDLINGGTALREWVDDPAATPGDLEAI
                     AAADEARWREERESLLLY"
BASE COUNT         4916 a        10560 c        10539 g         5060 t
ORIGIN      
        1 cacgatcgag gatcggtgcg gccggcaagc ccggaaaggc gaggaaggtg ggctgctgcc
       61 gcgggtcgtc cggctgcgcg cgctcttccc ggagatagat gcgcagcgcc cgatgcacgc
      121 gctcgagcgc cgcgcgccca tactgcgcgg ccagcggctc gagcgtctgc gccagcatgg
      181 cgcggcgccc gttgcgcacg gcccgagcag cgcgctcgac gagcggctgc agtcccgagg
      241 gagtagtcgc ggcatcgagc cagcgcccct gcgcctgcgc atcgcgcagc gcacgtgccc
      301 actgccacag cgcgcgcccg ccctgccccg cacgctcgag tgccgcgccg taattgagtc
      361 gcgccaggtg gaagcccggc gcaagtgcaa gcgcgctgtc ataccacgcg agggacgcga
      421 tgatattgcc gcccatttcg tgcgcccgcg cgaggtgata gcgcacgatc gtgtcggcgg
      481 gatcgacccg cgatgcatgg gtcagcttgg cgatcgcgcg caccgcatcg ccacgccgca
      541 gcgccgcgac cgccacgacc ttgagcgcct cgacgtcgtc gggatgggtc tcgagccgca
      601 gcatgcaggc ctgttcggcc gccagcacgt tgcccgaacg gaacatctgc aacggttcgc
      661 cgcgtggtgc gctcatgtgc gccggcctgt gctagcttgg tggccgccat ggcgctcgcc
      721 gaccttcgcc attacatacg cacctacgac gattccctcg atgcggcgct gtgcgcgcgc
      781 atgatcgcct cgttccacgg cctcgagcgc ttccacgcgg agaacggcgc caacgtgcgc
      841 cgcggcctcg aagagtcgcg ctggaccgaa ctcaacgtga gccgctacgc cgatgccgcg
      901 ttcctcggca tgttccgacg cacgatcgaa gcggcgctcg cgcgttacaa cgccgatgtg
      961 ggcctcggga tcgccgtgcc ggattccggg acgaactcgg acctcgtgat gaagcgctac
     1021 cggcccgggg gcggcgagaa gttccagctg catttcgatg ccgtcaacca tgtcgcgaat
     1081 cgctacctcg taatgctgtg gtacctgaac gatgtcgccg agggcggcga gacccgcttt
     1141 ccgcagctcg acttcgccat cgcgccgcgc gccggacgcc tgctggtgtt tccgccgtac
     1201 tggatgtacc agcacgaagg cgtgccgccc gcctcgggcg acaagtacat cgtctcgacc
     1261 tacctgctct tcaccgctca tccggcgtca tagccccagc gttcgagcag cggcgcgagc
     1321 agcggaagcg ccggctcgaa gtggcgccgg tacgcctgcc agcgacccac cgccctggca
     1381 ttcaccggct gcacgacctg cgagtagctc ggcgtgctga tgaaggcctt gcccagcgcg
     1441 tgctcggccg gcgcgagcag ggcgtcgtgc catggcagat cgagaaacgc agccagcgcg
     1501 cgcacctcgc gctcgaaatc ggtgacgagc ccctcgtagc gcaattcatg cacattcgcc
     1561 tgcagtgtcg cctgctcgcg gtaccagaag tcgaacgcct tgcggaaggc ggccgcgagc
     1621 cgcggcaggt cggcgcacag cagcgcgaac tcgggcgccg tgaagtgctg catgtgacag
     1681 ctgagcagca catcgcaggg atgtcggatc gcgaggatca cccgggcgtg cgggaagagg
     1741 cgccggatcg ccggcaggcg caacaggttg agcgggttct tgtcgacgag gcgctgaccg
     1801 ggggccagcg cgcacttctg ccgcgcgcgc tcgaagtagc ggcggcgtat cgcctcgaga
     1861 tcggccggcg cgatgacggc gagccggtcc ggatagcacc cgccggcggc cgccagttcc
     1921 tcgagcgcat gctgcaggca gggctgctcg tccatcgaga cgaggtgcgg gtgggcgtcg
     1981 aggacctgtt cgagcaatgt cgtgcccgag cgcgggaagg cgacgatgaa gaccgggctg
     2041 tccgccgcgg ccggcgcgcc cctcgtatcc cacgccgcga cgtcctggcg ctcactgcca
     2101 cgatcggcga tcgcgaacgt cggcgcgccg cgcagcgcgt gcaggggcgc cgccagcgac
     2161 acctgccgaa gctgcgagcg gtgcgcctcg ccgagtcgtt cccaggcctc atcggtgcgg
     2221 cccgcggcat cgagcgagcg cgcgagcggg aacaggtcgt agtgacgccg atgcgcaccc
     2281 gtctcgagcg ccagcgcccg ctcgaagtgc gcgatggcct cggcgtgtct gccggcgcgt
     2341 tgcgcaatcc tgccttctgc ggagctcaat gccgccgtcg gcgccgcacc gtgccggtgg
     2401 cgcagcgcgg ccagcaccgc gccggcgtcg gcgagccggt tcgcacgctc gagcatgtgc
     2461 accagcgtga ggcccgattg cagctcgagg tccggcagtg ccatcaggcg cgacatcgcc
     2521 gccgcggcga gcgccgactc gccggcgccc tgcaggcgct ggccggcatc ggccacctgc
     2581 gcaggcggca gggccgcgag cagctgctcg tcggcgaggg cgcgcacgcc ctcctccgtg
     2641 tgccccgatt cgaagcacgc attcgcatac tgcaggcgca tcgccgcatc ctgcggtgcg
     2701 agtgacacgc cgcgcgccaa taccgcgcgc gccgactcgt agtcgagccg gtcgaggtgg
     2761 gtgagcccga cgttgtagca gaacgcgggc gagaagccgc cgagcgcgtg cgcacgcgca
     2821 tacgcgcgca aggcatcgtc atagcgcgcg agtccgcgca gcgccgtgcc gaggttgagc
     2881 cagtgcgcgg cgcgcgtcgg ttgtagttcc gtcaagtggc ggaataccga ctcggccgta
     2941 tcgtagcgcc ccccggcgtg cgccgcggca cccaccatcg tgagtgccgc gacgtcgccg
     3001 gtccccgcag gcttcagaac ttctgcgtca cgccgagata ccactgccgc cccagcacgt
     3061 cgtaggtcga gacgtccgtg ttcgcgttcg tcacgttgtt ctggtagaag aacggcggct
     3121 cctcgtcggt caggttctgc acgccggcct gcagtcgcgt gttcgacgcg aagctgtaac
     3181 cgagcgtgag atcgagataa gtcaccgacg ggatgtcgag cgcctggtcg gggatcctgc
     3241 cgtcggcatc cttgatcgtg aggctgtcga agtagcgtgc ggtcagcgcg ccgtcgaagc
     3301 ctccgaacgt ccagccgacg ccggccaccc cgcgccactt ggcgtagttg ccgtactgcg
     3361 gatcgtaggt gcctgcgact tccaccggcg gcgcaccggg caccgggacg ctggtgaagc
     3421 tgttgatgcg ggtcgcttcg agctggaatg tccagctgcc gatcggtgta tcgcgcagcg
     3481 catagcgcac gcccacgtcg acgccgtccg tctccagcgt gccgagattg accgtcggca
     3541 actgaaacac ctgaaattga ccggcgctgg ggcccgactc gaagcggaac atcaggtcgc
     3601 agaaatcggt atcgccgttc tgcacgcact gatccgccgc gaagttcggg tcgatcgcgg
     3661 tgatgatgtc gtcaatcttg taccgccagt aatcgacatt gaacgacagg ccgcgaatca
     3721 ggctcgagtc gtacacgaac ccggccgtca gcacgtcacc cgtttccggc ttgagcagat
     3781 cgttgccggt cagcaggccc gtgatctggc tgttcggctg ctggaagccc gtgtccggcg
     3841 cgacacccac gcaggcgagc gcgagattcg ggttggcggc gagctgcgcc gaggtgagcc
     3901 cggcgcacgg atcgttgaag gtcgcggcgg tctgggtcgg cgcgagcgac aggtcgacga
     3961 tcgtcggggc ccggaagacc tcggcgtagg acgcgcgcag cagcaggtcc ctgaccgggc
     4021 gatactcgag cttcgcctgg aaggtcgtgt cgtcgccgat cgtgtccttg ctgtaatccg
     4081 aatagcgcac gccggcggtg aggttcaggg cctggacccc gggcaggtcc gcgagcagcg
     4141 gcacgaacag ctcgccgaac agctcgcgca cgtcgtagtc cgccgcgcca ttgccggtac
     4201 aggtctcctg tgccagcagg caggtgagga acaatggcgc ctcgccgcgc gtcagcgcgt
     4261 cggtctcgaa cacgccctcc tgctcgcggt actccgcccc gacggccgcc ttgattgcgc
     4321 ccgccggcat ctcgaacagg tcgccattga gtccggccga gaactgcgaa ctgcggaacg
     4381 tgtagtcggt gcggtagttg gtcgagatgc cgttcaacgc tgcgatctgc gccggatcct
     4441 caaggttgaa gatgttgatc ggtgtgcagc cggcgatgat cgccccgggt gtgccgcaat
     4501 gctccccgtc cgcgtcgacg aacgaaggcc cgagcgcctg gttcagcttg tccttcagca
     4561 ggtagccgct gatgttggtg ctctggccca tgtggccgat gcctgcattg acatcccatt
     4621 gccacgtcga ggcgaactcg ccacgcaggc cggcattggc gaagagctgg tcggaacgca
     4681 ccttgctgcg ccggttgccg agcgactcca ggcgcgcgcg catggccggg ttgtcgcccg
     4741 cgccgccgcc gaagtccgtc ccgaacgggt tgtagatgct gtcggccgag atgatcgtgt
     4801 catcggcgac cgcgtcgaac ggcagcgagg cgatcgcaaa acccgagcgt gtctggttgt
     4861 agacgacctg gccgtacgcc tccgccatgt cgttgatctc gtagttcacg ttcgtgaaca
     4921 ggctcccgcg ctcctgcggg gtcatcagca gattgaacgg ctggtagttg tacaggtcgc
     4981 ccgggatgtc gaagcagcgg aaatccgcga gcgaagtgcc gggcgcgccg tcggtgcgcg
     5041 tcaggaacgg gttgtcgcag ccgaactgcg cctgcgtcgc cgcgtcgacc tggatgaagc
     5101 cggtcggcgt gcgggacgaa ccgcccgcgg tcacgacgcc gccatagagg tacagcgcgg
     5161 tggccgagaa cgcccggtca ccggccgaga ccttctcctg cctgttgtag ttcgcgccga
     5221 acacgatgcc caactggtcg gtctggttgc cgaccgtgag gctcaccgag gtgcgctcgc
     5281 catcgctctc gccggtctgg ccgtactgcg cgccgatctc cgcgccgtcg aaatcgcgcc
     5341 gcgtgatgaa attgactacg cccgcgatgg cgtccgagcc gtagatcgcg ccggcgccct
     5401 ccttcagcac ctccacgcgc tcgatcatgt tgatcggcag catgttgatg tccaccgccg
     5461 aggtctgcgt gttgccgtag acgccgacgc gccggccgtc gaccagcacc agcgtgcgct
     5521 gcgcgccgag gcctcgcagt tcgacattcg actcgccggt gccgccgccg ttgttgacgg
     5581 ccgggttggt cgccgcaccc gcgacggtcg gcagccgctg caacacgtcg cccatggtcg
     5641 tgagtccggt cctggaaata tcctcggcgg aaatcgtgag caccgggctc gccgtttcgg
     5701 tgtcgaggcg ccggatgcgc gacccggtga cgatgacggt atcgatgtcg tccgtatccg
     5761 tggtggcctg gtcctgcgcc tgcgccgcag tgggcagggc gactgccgcc gcggcgcccg
     5821 cgcccgccat catcgacagg cgcacggcgt ggcgcaccaa cggattggtc tctggggaaa
     5881 tcatgaaact gcctcctgct gtcacgtgac ctgctccacg tgcgacgcaa gctagcagcg
     5941 ggaagttctg tccgggaacg gcacttgcga cgaaacaacg cagaaaacgc gatgaagcga
     6001 ggtgtcgtac cctggaagca acagtgcagg atcagccgcg gatgcggatt tcggcgaggc
     6061 ggaagtcgat caccaccgta tccatggcac cgagcacgtc catgccgatg atcagtgccg
     6121 gcacatcgtt cagcccccac aggtcaaaga catggaagtc gccgaaggca gcgacgacgt
     6181 cccggaaaat gacgcggtcc gccaggatgt ccggcacggc ggcgacgtcg ccagtcatcg
     6241 tggcctcggt cgtgccgtac accatggttt gccggccccg gtcatcggcg ctcaaggccg
     6301 cctgcagtgc gcgattgccg agtgtcttct gcgcgcccgt gtcgaagacc gccagcgcgg
     6361 gcacccgacc cacgcgaacc tgcgcgccga gcagcccgcc cttgacgcgc tcggcgcgga
     6421 tccgcagatc atcgccacgc tcgaagcgcg agcgaccgat cctgacgatg tcgcgcctga
     6481 aatcgacgaa cagacgctgt tcgcgcaggc ccgcgacacc gaggatgccg tcggcttcgc
     6541 ccatcaggtg cggcagcacc acgggcgcct gcaggtcatc gagcacgagc tcgcccgcat
     6601 gcacgcgggt gagttcgacc accgggactt cggcgctgcc ggtcacgccg ttcaggatga
     6661 cggtcggcgc gctgacgtaa tcgatgccga gccgcgtcgc gacctcggtc gtgatcgtgg
     6721 tgcgattggc gccggtgtcg acgatcagcc ggaagggccc ctcgccgttg atcatcaccg
     6781 gtaccaggat gcggcccacc tgatcgaacg tcgtcggcgt ggcaaaaacg gggctttcat
     6841 ccgcgaccgg cgcgagctcg gtggcgccca ggagttgcgg cgtggacagc gctccggcga
     6901 gcgcaaaggc cgcggcggca ttgcgcttca tgcgatcagt atgggcgccg cctccccggg
     6961 gtggcaacgc agcgcgggcg gctttcagcc ggagcgagcg ggtactgcgt cgaatgcgat
     7021 cgtgatgcgc gcatcgttgc catggatcgc attggtgccg tgccacatgt aggacggaaa
     7081 caggacgaga ctcccggcag agggccggat gtagcgctcg gccgtggcgc cgggggtggg
     7141 aatgcgcggc tcgccgaact tgatccagcc ggacatctgc gcatcgtccg cgacctcggc
     7201 cggcaccgaa acgtagtagg cagagctgat ccagccctcg ggatgcacgt ggttgacgtg
     7261 gaacccttcc ttttgcagcc gcacggacca ggcgccggcg aggcgcgcgg gcgccgtgtt
     7321 gcgcgcggaa agcggatgcg cggcatcggt tccgatcagc gcccgatagc tttcgatggg
     7381 gccctcgaaa gccttgatca gtgcccggat cgccgggtcc gggtcggtga gcaggctggc
     7441 cagggtctgg gtgccgtgac gcaggctctg gtcgagcgga tgcgtggcgg ccacgtggcg
     7501 cttcgcgagc gcctcgatcg cggctgcgtt cagttcctgc atcgacgacc atccctttgg
     7561 cgtctcgagc tcgaacttgc gcacgacgcg atcgtagtcg tacaactcct cgtagagcgc
     7621 cttgccgagc agccgcgcgg cggtcgcttc atgggcgatc cacaactgct cgttgggctg
     7681 gcgagcgcgc tgccccagga tgtatggcat ggcctcgtcc gccagccccc gcgccagcag
     7741 gatcgacacg aggggatcgt cgacggtcgg ctcgccgggc tgtgcccgcg ccgcttcgag
     7801 cgcctcgtgc tgcgcttccg ccggccgccc gctttcgaac agcaccatcg ccagcatcga
     7861 gcgcgcctgg ggcatcgatc ccctgcgcgc gagcagatcg cgcagcaacg cctccgcccc
     7921 ggcgaggtcc cctgcccgcc acagcacgcg gccaaacagc atctggattg cgacatcgtc
     7981 cggatgcgcg gcggcggcgg cgcgcacgga gcgggcgaag tccgcatcgc cgcgcaacca
     8041 gcgcaaccgg gccagattga gctgggcctc cacatggagg ggttgcagcg caaccgcggc
     8101 ctcgaaggcg cgttccgcct cgtccaggcg ggtgagctgc atgagtgcgc gaccgctgtt
     8161 gaactcgagt tcgaatccgc tcacgccgcg ctggcgtgcc tgctgaagtg cttggagcgc
     8221 ctcacccgcg cgttcgagtt gcgacaggag tgcgcccagg ttgtgatggg cggcgccctg
     8281 gcgcggatcg atctctatgg cccggcgaaa tgccgcttcc gactcttcta gccgatgctg
     8341 ggatcgcaac gcagtcgcga gcagcgacca gaccgcagcc tcggcggtcc cgctggcgat
     8401 cagcgtgcgg cattcggctt ccgcatcgcc gggtctgccg agatcgacca atgtgcgcgc
     8461 cagcgaaagg cgggccgcgc gattgcgtgg atcgagcgcg agcgcatcgc gatagcaggc
     8521 ctccgcctct tccatacgcc cgagccggcg cagcagactc gcgaggttcg cgcgaaactc
     8581 ggcggaacgc ggctctatcg caatgctctc gcgcatcagg cgctcgcaag ctgccacatc
     8641 gcccgcatcc ttgcggatca gtccgagcag gtggatcgcc gccgggtcgc gcgggtggcg
     8701 actgagtacc tggcggcacg tggtctcggc ggcggccagc tggccgccct gcaggagccg
     8761 cagcgcttcc ggcaacgagt gacccattgc gggcgtcacc ggctgcctag ccttgcccgg
     8821 tgcgcaacca tccgctgatg ctgtagcgac cgtagccggc gaacggcgcg accaggctga
     8881 ccgaatgagc ctggggcacg cggaagatcg tcagcgtgtt gaacgtgggg gcgtacgcct
     8941 ccgcgacatg tccgccgcgg gcgatgaaat tcagcagccc gccccagtcg gtccgccaga
     9001 ccggcgtcat gccgatgaca taggctgcga cccggttctt gttttcggca aggtcatcgt
     9061 gctgcgtcag gaagtgcccc gggccatagc acgtggcctg ggcatcgatg gtgtcgatcg
     9121 ccgcgactcc cgtgaccttg cgcgcgaaat cgaggaaggt cggcgaactc aggaattcga
     9181 atgcgcgcgc gacgatatat tcgcggccgc gcccggccgc gtattcgtcc gaaagcgggt
     9241 agttgcgata caggaactgg aagccgcccc tggcgcccgt gtgcacggcc gccatcagct
     9301 tcgcacgggc ctccgcgtcg agctccgcga gctggcgctc ggtgaggtca tagacgcctt
     9361 cgctgccgtt gaagtgcagc tgccagggga tctcctcggt gaggcacttg tacagggccc
     9421 gtgcagtctc cggatcgaac atgccgggca tcagcacgcg ccccaggcgc ccatagacgg
     9481 atgccatgac cgccggatcg accggtacgg ccagtctgaa cggcggctca gccgccccgg
     9541 ataccgcagg ttccgcgttg tcagccatgg cgcatcctcc cataaaaaaa cggcgggccg
     9601 aagcccgccg ttcagtacag ccttgcggct gcaatgcgat cagaacttgt agttcacgtt
     9661 gaagtagtac cgctgtccca gcacgtcgta caccgtcgga tcggtgttgg actgctggta
     9721 cgacgggaac agcaccgggt cctcgtccgt cagattgatg acgccggccc gcaaggacaa
     9781 gccgtccagc atgccttcct tgaagcggta gcccgccgac aggtcgacat aggtggtcgc
     9841 atcgagcttg aactccggga acgcgatatc gtccatctcg ccgacgtagc ggaggcgtgc
     9901 cagcacgtcg aagcccgccc agtcgtagcc gaggttcagc gtgaacttct cctcggggaa
     9961 cgaaccaccc gcgccgtagt agccgctggt gcccgcgagc tcggtgaacg cgtcgcccgg
    10021 gacttcctgc gtgtcccact tgtcgaggaa ggtcatcagc cagttgacgc gtgcacggcc
    10081 cgggccgaga tcgccgaccc agtcgagctg cacgtcgtag ccggctgcct ggtaggcacc
    10141 gatgttcgag ttcgcttcca gtgccacttc gatctgacct gacgacgtgt tgcgaggtcc
    10201 gaccaactgg cagaacaggt tgttgacgtc gaacgtcggg ttgtacgccg ggtcgtagca
    10261 gcggctgacg aaggtgcggg ccggaatcga gcccaccgcg ttctcgatct cgatgtccca
    10321 gtagtccacc gtcaggctca ggcccgacag gaacgcgttg tcggacggcg agcgcagcac
    10381 gaagcccacc gtgaacgtat cggcggtttc ctcggtcagc agcggattgc cgccggagag
    10441 gccctgcacc tggctgccgc cgaagctgta gatatcgatc aggcccgccc cgataccctg
    10501 atccatgcac agctggcgaa ccgctgcagc gttcgcaccc agacgctccg ggctgttgat
    10561 gctgcacgga tcaggcggag tgtacgccgg gaagttcggc acctgcggct ggaacagctc
    10621 ggtgacactc ggctggcgca cggcacgctg gtacgagccg cgcaggctca gtgcctcgac
    10681 cggacgccag accagttcgc ccttgtagct gtcgacgcca ccgatacccg acatgtcggc
    10741 atagcgatag ccgagcgaca cgtcgagcga cttggcgccc ggcatgtcct tgagcaccgg
    10801 gacgagagct tcgacgaaga actcggtcga gtcgatcttg ccgctgatcg gcagcgccgc
    10861 gttgaagccg gcgatgtcga agcgcgcgcc actgtacggc gtggccggaa tgactctcgc
    10921 cagtgcgtcg tccgggatga aggcgtattc gtcttccttg tagaaggcgc cgaacgccat
    10981 gttgaccgag ccggcaggca ggtcgaacac cggaccgctg atcgtcgctt cgaagatcgt
    11041 ctggtcgatc tcttcgcgcg ggttggtgtc caccgtgatg tagtcgacac attcctgcgt
    11101 aatggcgccc gcaccgaacg gattgaagcc accgcacagc gcaacgccgc catcgggcgc
    11161 gaaggtcagc tcttcccact tctcgcggct gatgttgccg gactgcggcg agtcgacgtc
    11221 gacattgccg atggcaccgt acacatcgta gttccacgag tcgaagatcg agcccttgaa
    11281 gccgagcacg acctggtagg cctcggagtt gtacgtctgc acgcgtccgc ccgcctcggt
    11341 catgcggcgg gtgagggtga acggcgcatt cgggtcggcc cttgacgcca gcagggtggc
    11401 gaggtcggcc ggtacgaacg gattggtcgg cggcgtatac acggtgctca gcggagtcgg
    11461 cgccaggaac tgctcgccgg tcatgtcgct gtagagcacc tggccataca cgctgaacga
    11521 ttcggtgacg tcgaacccgg cgtgggcgaa cgcggtggtg cgctcgagcg gcagctgcag
    11581 gtagttggcc ggggcgaagt tgtacgtgta gttcgcgtcg ttgaaggtgt cggccgccga
    11641 atcgcccttg aagttcagca ccgaaccggg ggtgccgtcg cccaccgtga acagggtgcc
    11701 gtcgctgttg aacccgtaag tggcctgggc aattcccgcc gggagcccgt agctgccaaa
    11761 caccgcatca cgcgctgcct gcgacgcagc gacggtaacg cggccttcct cgaacgtggg
    11821 cgagccgttg ggcgagaagc cggggtcgtc gtagccgagc gcgacgcggc tgtggctgcg
    11881 ggcgcccgcg aacacttcct cgcgctccgt gtacgacacc gagcccatga tgtggccgcg
    11941 atcctcggcg aagttggtgc cggcggtcag ggtggcgaag tactcctggc cgtcgctttc
    12001 gctggtctgg ccccaggtgc cgtcgagttc gaggccctgg aagtccttct tgagccggaa
    12061 gttcaccacg cccgagatgg cgtccgaacc gtacacggcg gacgcgccgc cggagacgat
    12121 ctcgaccgtc tcgacgagct gcgacggcag gatgttgagg tcgacgatgc cgctgccgtt
    12181 cgacgggacg atgcgccggc cgtccatcag gacgagcacg cgctggctgc cgaggccgcg
    12241 caggtcgatg ttcgactggc cgccgttcga cgggttgttg gacgtattgg tgatggacgg
    12301 aacgaactgc ggcagcgagt tgatcacctg ctcgacagtc gccgtacccg tctgcgcgaa
    12361 cacctggctg tcgaccgaga cggtcgggct cggcgattcg aagtcggggc gggcgatacg
    12421 cgatccggtg acaaccaccg tatcgatcgt tgcctcgtcc tgcgcctgca cttgtgccgg
    12481 cagcgctgtg gcagccgcta ctgcactcat caagagtgtc cgacgcacgg cgcgccggat
    12541 ctggctgttt ctctccatca gatgaatcct cccgaaaacg gcaacgccgt tcatacttgc
    12601 tggaagcaac attagttttg accctgatcg cgatgcatgg aacggctatg ccgcccctgc
    12661 gccgctttgg agcggatatt aacaactatc cgacacggca caagccccgt agcaggaaaa
    12721 caacgccaag tcctgcgctg cggcaacggt tcgtcgcatc tggcagccgt gctgacccca
    12781 aaaaacaacg cccggtgggc cgggattgcg acgaagcgct tttcggttgc gatccagcaa
    12841 cagtgggatt cctaccacta cctgcggcgt gctgcagccc gcgttctaga atcgcgcaat
    12901 gccccctcga tccgctgccc tgaccgaggg gcccatcgcc cgctcgctga tcgtgttcgc
    12961 gctgcccatg ctcgcgggca acgtgctgca gtcgctcaat ggctcggtca acgcgatctg
    13021 ggtcggcagc ttcctcggcg aagccgcgct caccgcgacc tccaactcga acaccgtcat
    13081 gttcctgctg atcggctcga tgttcggcat gtctatggcc gccaacatcc tgattgccca
    13141 gcactttggc gcaaggaggc tcgccgaggc gcgccgtgtc gtcggctcga gcgcttcgtt
    13201 cttcgccgcc gtggcgatcg cagtcgcggt gctcggcgtg ctcttttcag accacatcct
    13261 tcgcgcgatg cacacgccgc ccgacgccct gccactcgca catgattacc tgcggatcat
    13321 cttcgtcgcg atcccgttca tgttcatgta cgcgttcgcg atggcggtgc tgcgcggcgc
    13381 cggcgattcg cgcacgccct tcctgttcct gctgctgtcg gtcggtctcg acatcctgct
    13441 gaacccgttg ctgatcttcg gcgtgggtcc gctgccgcgg ctcggcatcg caggctccgc
    13501 ccttgcgacg ctcatcgcga acatcgtcag cctcggagcg ctggtggcgc acctctaccg
    13561 caagcgtcac ccgctcgtac tgcaccgcga cgacctgcac ctgctgcgca tcgaccgcgg
    13621 gatcatcgcc acgctgctcg gcaagggcgt gccgatggga ctgcagatgg tggtggtgtc
    13681 ggccagcgcg ctgctgatgc tcgggctgat caatcagtac ggttcgcaga ccaccgctgc
    13741 gttcgccgcc tgcatgcagg tgtggagcta tgtgcagatg ccggcgttcg cgatgggcgc
    13801 ggccgtctcg gcgatggccg cgcagaacgt cggggccggc cactggggtc gtgtcgggcg
    13861 catcgcgatg gccggcgtca ctttccagac gctggtgacc gcggtgtcgg tgctcctgat
    13921 cacggtgttc gcgcgcccgt tgctcgggat cttcctgccg gccgacagcc cgtcgctcgc
    13981 gacggcggtg cacgtgaact acgtcgcttc ctggtcgttc gtgctgttcg gcgtcacgat
    14041 cgtgttgttc ggcgtcgtgc gttcgaccgg cgcggtcatt gtgccgctcg tgatcctagt
    14101 catcacgctg tggggactgc gcttcccggc ggcggcaagc ctgatggggc gctacggcga
    14161 ggacgcgatc tggtggagct tcccggcgtc ctcggcgctc tcgacgatgc tcgcggtggc
    14221 gtactaccgc tatggcggct ggcacaaggc caggatgtaa gggctggcat tcattccttc
    14281 gcgccgactc cggcgatgac gccgctcgcc ggcttgcggc ccgcagccgc ggcggcatcg
    14341 aacgcgatcg gcgtcgtata cccggcgtcg actttcgcga acggcaggcc gttcaccgcc
    14401 gcggtggtgc tctcgcacag ctcgagcgtg ggcagcttcg gcaacttcgc gtccacctcg
    14461 ggaatctcga cgttcactgc gctcacggtg agcccgagcg caccggcaag gcgctgtacg
    14521 agttcggcga gttccttgcg gaagtcctcc acgagcgatg aatcgcgtcg ctggtaatag
    14581 acgctgaggt aacgcgcaat cggcgaggcc cgcgcgacct cgcgattgac gagttcctgc
    14641 tcgttcgcaa agttgaacca cggcgaggtc gtcaggtagt cgatgaagtc ggtcggaatc
    14701 gcgggctcgt ctgcccggat cgcgccggtc ttcaccgtca gcacgtcgcc ttcgcgtgtg
    14761 atctcgacat cggcgaggcc gagctcgtag gagagcttgc cgggccacgc gacgaagatt
    14821 tccggatcga tgccgacgac ctccgggcag gagcggtacc agatcgcgat ctggcgctcg
    14881 tgctcgatga tcgcatgcgt cctgacctgc gtgacgatca cctggaccgg gtcacccgcg
    14941 ggcgcaccct gccaccagcc gcgcgccaac agtatgccga ggaccagcgc cagcgcggcg
    15001 acgagcagag tgcgcatcga ctactccttc aggtacttcg cgagccaggc ctgcacttcg
    15061 gcatagaaaa agcggctgtt ctcgcccgcg aggatccagt ggttctcgtc cgggaacacg
    15121 agcagccggc tcggcacctg ctggcgctgc agcacgctcc agtactcgag cgtgttgttg
    15181 agcggcacgc ggaaatcgcg ctcaccgatc gtgacgagta ccggcgtcct gaaccggtcc
    15241 gcataggtga gcggactctg ttcctgccac agcgcctcgc cactccatgg cgtactgccg
    15301 atattcactt cgcggtggta gatcacgtcg ctcgtggccc actgcgagcg caggtccagc
    15361 agcccggcgt ggctcacgag gcagcggtag cgcgtggtac tcgcctgcat ccagttggct
    15421 aggtgaccgc cgtaactcgc gccggccgcg cactggcggc tggcatcgat gaaggcgaaa
    15481 cgccggatcg cctcgtcggc ggcatcgttg atgtcgtcgg ccgggccgcg caggggatcg
    15541 ccctggatgc tctgcgcgaa cgcctcaccg taaccggtcg agcccttgta gtcggtcaga
    15601 agcacgacgt aaccgggctg cgccagcagg tgatagttcc agcgcaccac gaactggtcg
    15661 gtccacatgc cgtgcggccc gccgtgcatc agcacgagga gcggatactt cttcgccgga
    15721 tcgaagccgg gcggcagcgc catgaagctg tggatggtgt cgccgcgccg attctcgaac
    15781 gtgaactcgc gcagcggctg ccagtcgatc tgcgccgcga gcgcggcgtt gaagcccgtc
    15841 agcagggtgt gccggcgcgc ccgcggctcg agccgcacga cctccggcgg atgggtcgca
    15901 ctgtcccagg tcgcgaccag caccggcgac ttcgccttgg cggcaagcgc gagcgagccg
    15961 tagttgccgc tctcgagctc gtaggcgagt cgcggcgccg cacgatgggt cggcgtgctg
    16021 taaagcttgt ggcgaccgga atcaccggcg aggaaccaca gtgtgcgtcc gtcggccgac
    16081 accgcaaacg acgtggccgg gcgatcgagt ggcgcggtca gcacgagggg cgcaccgacc
    16141 gcgggccaat cgagcgcgac gacctggtcg atgttgtagg gcttgccggt gccgcgctcg
    16201 aaggctgcga acaggcgccg accatcgggc gagaacgccg gattgcggta acggtccggt
    16261 ccccgcgtca ggcgtacggg ttcggcgccg ccggcggccg gcacgcggta gagctgcgtg
    16321 tcggtgaacg cgaacgccgc ctggttgtgg ttctcgctgg cgacgaacac gagctcgcgc
    16381 gaatcgcgcg tccaggcggg gtcgagattg tcgccggcat tgccaccccg gccggcgaag
    16441 ccgggcgcgg ctgcgagctt cgtgccggcg agcaggtcgc gcgcctcgcc cgccggattg
    16501 ccggccgccg tgagcggctg cacgaacagg tggggccgct gcggcttgcg ccagtggtcc
    16561 cagctgcgga tcggaaagcc gtcgtagacg cgcgcgttgt atttcggctc cgccgccgcc
    16621 gccggatcgt ccggcacatc gctcacgaag gcgaggctcg cgccgtcggg cgccagtacc
    16681 ggcgagcgcg cgccggtact ggcggcggtc agacgccgcg cctcgccgcc gaaaccgaga
    16741 tcgaggatgt agatctgcgc ggccttgtcc tcgtcgcgct ttgcgctgaa gacgatgcgc
    16801 tgactgtcct cgctccaggc gacgtcgctc tcgccgccgc ggctgttcgt caggcgccgc
    16861 gccggagcac tgccgtccgc cggcacgagc cacaggtccg acagcctgtc ctcgtccgca
    16921 taggcgggct ccgcgaccgt gaacaccacc cagcggccgt ccggactcgg taccggatcg
    16981 ccgatgcgct tggcgagcca gacatcctca tgggtgatcg gacgcaggtc cgcggcctgc
    17041 acggcggcgc agcagcagaa tgcaacggcg aggatcagcg ctcttttcat gtcgtctcct
    17101 ccggaagccg ggccaccttg ccggaccccg gggcgcgacg tcaacgcggg cgctcctcca
    17161 gcggaccgga cgccggcaac cagacgctgg cgagcgcccc gccaccctcg cgattcgcga
    17221 ggatcaggtg gccaccgtgc gcatcgacga tttcgcggca gatcgtcagt ccgagcccgg
    17281 taccgctcgc cttggtcgag aaaaacggca gcagcgcgga agtgagcgcc tcgccggaca
    17341 atccggcgcc gcggtcgtac acctcgagca ccacgccgcc acgccgggcg cgcacttcga
    17401 gttccacgtc gctggcggcc gaaccggact cgagcgcatt cttcagcagg ttgatcgcca
    17461 cctgctcgag ctgcgatgca tcggcgtgca ccgtgatgtg cggtggctca ccgcgcagcg
    17521 tgaactgcgc caccgcctgc aaccgcgaaa cgagcgcggg ccagtcgagc ggcgcaagac
    17581 gcggcttcgg cagcttggcg aagcgcgcgt agccgtcgat gaagcccgcc aggtgcgtcg
    17641 cccgctcctc gatcgtcgag aacaccttgc gcagcggcac cgtatcgggc gcctcgagca
    17701 gcagttgccc ggaatgcgcg agcgaggaga tgggcgcgag cgagttgttc agttcgtgcg
    17761 cgatcacgcg gatcaccttc ttccacacca cgacttcctg tgcggcgagc tcgcgcgtga
    17821 ggcgcttcag cagcagcagc cggtgcggct gcgtgttcag ctggaaatgg cgctgcgaga
    17881 ggtggtagac ctgcggttcg ccaccgagct cgacggtgaa cagccggtcc tgtgccgtcg
    17941 ccagcgcttc gcgcaacggg cccggcgcac gcgcgagggt cgcggcgaaa tcggcgccct
    18001 cgagccggtg cccgcccgcg aacagctcgc gcgccgccac gttgctgtag atcacggtgc
    18061 cggcggcatt ggtcagcacc agcgcaagcg gcgtcgcctg gatgaccgtg tcgagcagca
    18121 gttcgcgctg gtacaggtcg acgcgttcgc gcctcaacag gtcaccgagc gagttgtacg
    18181 cttcgacgag ttcgccgagt tcatcgtccg aagtgcgcgt gatggagacg ctgaagtcgc
    18241 ggtcgcgcag gctcgcgatg ccgtcgcgca gcgcgatcgc cgtggcggtc cagcggcgcg
    18301 cgacgccgcg tgccagccag acgctcgcac agatgccgac gagcaacccg cacagcatgg
    18361 cggcccaggg cgtgagccag cggaccgcga gcagcgccga gccggccgcc agcagcacgg
    18421 caagggcaag cacggcgagc agcgtgccct cgagtgaacg gcgcatcaga gcagcgggta
    18481 gggctttgcc cgcacccact tcgagtacag ccacttctcg ccccgggtcg gtggcagccc
    18541 ggcatgcagg gtgcgcgggt ccggctcgcc ttccggcgtg gtgttccaga acatcagcgc
    18601 atcgccggtg ccgcccttgt agcgccagcc gagttccggg aagtcggttt caccaccttc
    18661 gtagtcgtcg ttcaggtaga tgagcaccgt caccgtgcgc tggcctgcga cctgcaggtc
    18721 gccgacgtgc ggctccgcct gcgtgaagta atcgtagtgc gcgcgatact cctcgccggg
    18781 ggcatagtgc agcacgttgg tgggctcctg gtgcgagagc ggcacaccga tcgccgcgcc
    18841 gacacgggca ttgaccatct gcagcagcag gtccgattcg acgaggctga agcccgtgcc
    18901 gctattggtg cggacatcca tctggcgcac ctcgccggtc gccgggtcct gcacgcgcgc
    18961 agcgtcgagc ttccagcgcg cacggccgat gatccagtcg cagaaggcac gcgggatgca
    19021 ccgctcgatg atgccgatgc gcggcgattc ggactgccac cgcggcgcga gcggcaggtt
    19081 ccattgttca cggtcgaact gcgccggatt ggccagaatc ccggcctgca cacgcgccag
    19141 cggttcgccg gcatcggctg cacgccgcag cagcttgaac gcctcgggcc agtcccccga
    19201 aaggctggtg agcaccgcca gcagcgcgag cgcctgcgga tggcgctggc tggccgccgc
    19261 ctggacccat ttcaggccct gcggaggcgc gaacggtgcc ccgcgcccga cgagcaggcg
    19321 tgcgccaagc tggtattgcg cctgggcatt gcccgcttcg gcgccttgtt gcagcgactc
    19381 catttcaggc agtcgacttg aggccgagtt tctccatacg gcgatagagt gcctgacgag
    19441 agagcccgag gtcacgcgcc gcgtgcgcta cgaccccgga gcagcgcgcg agcgcctgtt
    19501 cgatcgtgtc gcggtcgagc gtggcctcct cgggcgccgg cgggacgcca ggcactgccg
    19561 gcaggccgag cgccgcggcg gtgatcatcg ggccgtcggc gagcagcgcg gcgcgctgca
    19621 tcgtgttgcg cagctcgcgc acgttgccgg gccagccgta gcgcagcagc gcgcgctcgg
    19681 catcgggcga cagcgacagg tcggcgtcga ggaaatggcg cgcgagcggc aggatgtcgt
    19741 cgcgccgctc ggcgagcgaa ggcacctcga gctcgatcac gttcaggcgg aagtacaggt
    19801 cctcgcggaa gcgaccctcg cggatcgcct ggcgcaggtt ggtgttggtc gcggcgatca
    19861 cgcgcacgcg cacgcggcgg gtctggttcg agccgaggcg ttcgaactcg ccggtctgca
    19921 ggacccgcag caatttcgcc tgcccgctcg cggacaggtt gccgatctcg tcgagcagca
    19981 gcgtgccgcc atcggcggcc tcgaaacgcc ccggccgcgc gcgtgcgccg gtgaaggcgc
    20041 cggcttcggt gccgaacagc tccgcttcga tcaggtcgtc cggcagcgcg ccgatgttca
    20101 ccttgacgta gggaccgcgc cgcacggcgg agttcgcctg cacgatgtcg gcgatgccct
    20161 ccttgccggc gccgttcggg cccgtgatca gcaccggcac gccggcctgc gcgacctgcg
    20221 ccgccatcga caccaccacg tgcagccgct cgctcgcgta gacgatgccg gcaagatcgt
    20281 ggcgcgcggc gagcgccgag cgcgccgcgg cgcgctcgct gcgcagcgcc tgcatgtcgg
    20341 cctgcgcgcg ctgcaggtcg aggaggttgc gcaccgaggt cagcaggcgc gcgtcctccc
    20401 acggcttggc gacatagtcg gcagcgcctt ccttgacgag ttccaccgcg gtctcgagat
    20461 gggtccatgc agtcagcagg atcacgggca gctcgggccg ggacgcgcgg atcgcgcgaa
    20521 acagtcgcac gccctcctcg ccggaggtcg cgtggcgctg gaagttcatg tcctggatca
    20581 cgaggtgcac cggctcgcgc gcgagcgtcg ccagcccctc gcccggcgag ccggcgacca
    20641 gcgtgcgcac gccgtggatc gacagcagca cctcgaacgc ggtgcgcacg gcctcgttgt
    20701 cgtcgatcac gaggacggtc cggttcatca ggctgtcgcg cagggcgtcc cgggtggctt
    20761 gtctcatgat tcggatgctg ctctggcgca aaaggttgca gtgttacggt tcagaccgtt
    20821 cgcgtggcca ccgacggcga gatccgcgcc gccctgcgcg agggctgcca ggccgccgcg
    20881 aggcccagcg cccacagcag cagcacgccc cccagcagat agtacaggtc caggcgcggc
    20941 agctcatact gggtcgacag ccagtagcct acgccgaagg caagcgccac cccgagcagc
    21001 acgccgccgg tcgtgacgag ccagttctcg accatgaaat agcgcacgat gtcggcgcga
    21061 cgcgcgccga tcgcccgacg ggtgccgatc tggcgcgtgc gaacgttcac gttgaaggtc
    21121 gcgagcgcga agatcccgag cgccgtgaca ccggccagca gcgcggtgac cgacacgagg
    21181 aagatcgcca tgttgcggtc cgagagatag ctgcggtcgc ggtagtactc gagcgcgcgc
    21241 acgcccctga ccatccgggt ggggtcggag gtcgcgaggt attcctcggc cttgcgcatc
    21301 aactcgtcgc gcgcccccgg ggcggcgcgc acgaggtaga gattgtcgcc gccgaacgcg
    21361 ggcgtcatgg gcagcaatgc cacctgatcg accctgtccc aggaaaccca ggagccgtgc
    21421 atgtgctcga tgacgccgac gatcgtcgtc gggcgattca tgtagtcgta ggccaccttg
    21481 ccgacggcgt ccgtcgtgcc gaacagctca cgcgcgaccg acttcgtgat gatcgcgacc
    21541 ggtggtgcct ggctgcggtt cggatcgggc ggcatgattt cctcggcgcg gaagttgcgg
    21601 cccgccacga gctgcacgcc gaacgtttcg agaccgccct cgtcgacttc gaaatagttg
    21661 atcgccacgg tcttgtcatt gggcgcatcc ggctcgctcc ggatgcccgt cgaggatccg
    21721 ccgccactca gcggaatcgc attggtgcgc gtcgcggcca cgacgccggg ctgcgaccgc
    21781 agccacgcca ggtcgttgcg cgcggcggcg agccggtcgt ggtcctgcgt aaagccggaa
    21841 ctgatgaccg tgaagacgtt cgcgacgtcc atgccggtcg gccgcccgat caggtcgaca
    21901 cgctgcttga cgatgtagat cgcgttgacg agcacggcga gcgcgatcgc cacctgcaat
    21961 gatacgagga tggcaccggt gcgattgcgc agcagcgcgg aaagcacggg gcggatgtca
    22021 ctcatcacca tgatcggatc ctcgcttcag gacttcaggt agacggccgg ggcaagacga
    22081 cccacgcgcc atgcgggata gaggccggcg gcgagcgccg acacgacggc gagcgccacg
    22141 gccatcgcaa cgctccagac gtcgatgtac atgacggtgc gaaacccgct gctgcctgtc
    22201 gcgtacatcg cgcgcaggcc cagcagtccg agactcgcca gcacgaggcc cgcggtcgcg
    22261 ccgaccgccg cgagcattcc cgcctcggtg agcagttgca ggaagatctg gcgccggctc
    22321 gcgccgagcg cgcggcggat gcccgtgatc gtggcgccgc ccaggaactt cgcgagcagc
    22381 aggcccacgg tgttgatcag gcacaccgac aggaaggcga acgccatcgc gaccagcatg
    22441 cgattgtcat tgcggaccac gtcgttgtcg gcgagccact ggtcgaccgg ggtcaggcgg
    22501 ttgttgtcgg gccgcgcgaa gcggccgctg ctgcgctgct cgttgacgta gccgtcgagg
    22561 tactcctgca tgcgcacccg cgtggcggca tccggcagtt ccacccaggc ctgcacccac
    22621 acgcactcgg aattcaggaa atcgagatag ctctcgcgct gctcgggctt ccagcagttg
    22681 gtattgccga cgctgtccag ctcgagcgcg ggcaaccagc cgaacggcac gtaggcatcc
    22741 tccgggtcgt cgaagctgcc gttgttcagg tcgtagaact tgggcgaggg atgccagtcc
    22801 tcgagcacgc cgacgacgcg gaactcgcgg tcgttccagc gcagcgtgcg ccctacgctg
    22861 ttcgcgccgg cgaagaagtg ctcgttcatc gccttgctga gcacgatgac gggctccggg
    22921 cccgtgtccg ccgcggcctg ccagccgcca ccgtgggcga acggcacgtc gaacatcccg
    22981 aagaaacccg ccgtcgtcac gcgcagcagc gcgcgttcgg gcagcacatc cgaggccgcc
    23041 tcggcgctcg agagcacgcc gcccgtcctc atcatgcgca cgctgcgcgc cgctatcgcg
    23101 gttgacgtaa ccgcttcggc atcgcggtac gtcagctgcg gtggcggcag ctcgggccgc
    23161 tcctcgttgg ccggctcttc ggcatcccag ctgtcgagcg tgatcgcgta cagccggtcg
    23221 ttcttccacc agatcgggtt cgaggacatg ccgtgataga cggtcagcgt catcacgcac
    23281 accgcgatgc cgagcgcgat cgccatcacc atcagcgcgc tgatcccggg attgcgccgc
    23341 agactgcgca gcgcgagctt cacgtagtag ccgaacatgg tgtgctcctc aggccatcgc
    23401 cgccgacgcc atgacttgcg gctcgcgcgg ctcgtacggc ttgtagtccg agacctggcc
    23461 gtcgatgacc tgcacgttgc ggtgcgcgcg ccgcgcgagt tccgggtcgt gggtcacgag
    23521 cacgatggtg gtgcccatct cgttgatctg ctcgagcaga tccatcacct ggcgcgccat
    23581 cagcgagtcg aggttgccgg tcggctcgtc ggcgagcagg aatttcgggt cgcctgcgat
    23641 cgcccgcgcg atcgcgacgc gctgctgctg gccacccgag agctgcgagg gcatgtggtg
    23701 cgcacgcgag ctcagtccga cgatgtcgag ggcgctttcg atgcgttcct tgcgctcggc
    23761 ggtcttcata cgccggtatc gcagcggcac gtcgacgttg tcgaagatgt cgaggtccgg
    23821 aatcaggttg aagctctgga agatgaagcc gatcttctcg ttgcgcagcc gcgagcgcgt
    23881 gtcgtccgac aggccgttca cgtcgacgcc gtcgagctcg tagcggccgg tgtcgaaggt
    23941 ctcgagcagg ccggcaacgt tcaggaacgt ggtcttgccc gagcccgacg gcccggtcac
    24001 cgcgacgaac tccccggatg cgacgtcgag ggagaaatcg cgcagcgcgt gcgtctcgat
    24061 gagctcggtg cggaagactt tgctgatgtg gcgcatggca agcatggaaa tcatcctctc
    24121 tggtcggtca gtcggtgatg agtacttcag gggcgccgcg gaaatcgcgc gtatcggaaa
    24181 tgatcacgcg atcccccggt tcgaggccct gcaacgcctc gatcttcgaa atcgacgcgc
    24241 cgccgagctg tacgggcacg cgcaccgcgc gctcgccgcg cacgacgtac accgaggtgc
    24301 tcgtctcgcc gagctgcgag cctcggtcga acgcgagcac atcgttccgc tcgtccagta
    24361 cgatgcgtac actcgcccgc tggttctgtc gcaatgacgg cggatcctcg ccggcgaact
    24421 tgagccgccc ggtaacctgg ctctggcgca cttccggcga aatcgacgcg accgtgccgg
    24481 cgtgggtgcg gccatcgagc gtgatctcgg ccgccatgcc cgggcggatt tccgtggcat
    24541 aggtttcggc cacctggaac tcgacctcga ataccccgag atcgacgacc gtgacgagcg
    24601 gcgcgttttc ggcaacattg gcccgatcgg tctgcgccag gttcgcaacc gtgccgtcga
    24661 cgggcgagcg cacggtcaac tcatcgacgc ggcgccgcag gttttccacc accagcgcct
    24721 ggcgatcgcg ctcgaggcga cgcgttcgca gctcgagctc caggctgtcg cgctcgaggc
    24781 tggcggtcgc ctgcgcgtgc tcgaaattca gccgcgccgt ggcgacatcg tcctcggcct
    24841 ggcgcagttc gcgctcggga atcgcgcgcg tctcccatgc ggcccgtgcg cgtgccagct
    24901 cgcgttcggc ggcacggatc tgcacgtcgg cgagatcggc ctgctgctgg ttcagcaggt
    24961 tctggcggcg gacttcgatc gattcgcggg cgagcgccgc ctcggcgccg gcaagattcg
    25021 cgcgttcacg ctcgtactcg ttgcgcagcg cggggctgtc gagtgtcgcg atgacatcgc
    25081 ccctggcgac cacctggccg gcgtgcaccg cataggaaat cgtgccgggc gccgaggcga
    25141 acagtgtcgg gctcacggcc gcgatgaccg tgccctgtgc cgccacgtcg cgcacgaaat
    25201 ggccgcgttc caccgtcgcg atgcgcagcc gctcgagagg cacggtattg cccgccgcga
    25261 accagccgcg cacgccccag acgagcgcga gcagcagcag cacggccgca ccgccggcga
    25321 tctgaagctt ccggtggcgc cgccatcccg gccgcgaagg ctcgcgggcg acatcctgcg
    25381 aggcggtgcc gcggatcgcg cccgccgcgg gatgctcggt gctcgtgtcg ccgggccgac
    25441 gcagtgcgga aatcttcatg cactacgtat tgcagcatgc gtgccaaccc cggggtcaca
    25501 ctcaagtcac tgaatggaaa agcatttcac ttcacggcga tgatgcagat gggccgggtc
    25561 tgcatgtccg gcagcggaca ccggacacgt ccgcggacat ccacggtcgc cgctgcggcg
    25621 gcactgagac ctgagtagtg gaggaactca tacggcgaca tgccgatcgc ggtgatcagc
    25681 gcggcggcga tgagtgcgat gatgcgcatc cctgcaaact tgatggtcat gttccctccg
    25741 tgggtggcga gttggcagca atccctgcgc tgttactctc tcccggcggg cactccggaa
    25801 caaaccgact ttctgaagcg caattaatgg aagcttgccc atggatgcgg cctacgatcg
    25861 cctgcccctg aatgcactgc gtgtcttcga ggccgtcgcg acgcggctca atttcgccga
    25921 cgcggccgag gcgctgcacg tcacgcccgc agccgtgagc cagcaggtca aggcgctcga
    25981 agactacctg cagacaccgc tcttccggcg acggggccgc agcatcgcac tcaccagcga
    26041 aggggccgac ctgctgccgc gcgtgcgacg cgggctcgat gagctcgaag cggccctgca
    26101 gcaatcgcgc aggcagcgcg agtcgggcgt gctcaatatc agcatgctgt cgtcgttcct
    26161 gcagaagtgg ttcgcgccgc ggctcggcgc gcagcacgat catctgcacg acatcgaatt
    26221 gcgcgtgcac acctcgcgcg aggcggtgga ttttgcgcgc agcgacttcc acgcggcgat
    26281 ccgccttggt gccggcaact atcccgggct ctattccgag aaactgctcg acgactggct
    26341 ggtgccggtg gcgacaccgg cgctcgtgcg caagtacggc atgattccgc gggcgaagga
    26401 tctgagcgcc tttccgttgc tgtacagctc ggacgagccc tggtcgtcat tcgtggccga
    26461 tgccacggcg ggctcggcga aaaccaggcc cgcgttgctc gacgactcgg tgtcggtgat
    26521 ctccgctgcg ctcgaagggc atggcttcgc ggtcgcgcgc tggtcactgg tgcaacggga
    26581 cgtcgccgcc ggcaggctcg ccatcgcctg cgacgtcgtg cgccagttcc gcttctccta
    26641 ttatttcgtg tgcccggact cgtacgcggc catgcccaag gtcgtgcgcc tgcgcgactg
    26701 gctgcgcgcc gaggcagccc ggcacccgtt accaccgacc atggctctga gcctcaagcc
    26761 ctgagccgca gggcccgagc ccatctcgcg ccgagccgat ccggttgcac gacgacccga
    26821 gcaaccgcgg cgcgcccgcg cgcatccaat ccagcgagac acaggcaatc accccccgat
    26881 acctggaaag gaaagagtca tgaaacggat catcttcggt gcgggcgcac tcgccgcaat
    26941 ggccttcagc gccgcggtct tcgccgacgc gggtgagcgg gtcaccgtgc gcgaagtgcc
    27001 cgtgcactac agcgacctca atgcgaacag ctcgcagggt gcgcagcagc tctattcgcg
    27061 gctgaagtca gccgcgaggg cggcctgcgg caacgagccc ggccgtcgca tggaggagcg
    27121 catcgcctac gacaactgcc gcatggatgc gctctcgaag gccgtgtcgg aggtcaacag
    27181 caggacgctg acggcactgc atcgctcgcg gattgcgccg ctcgaactgg ctcgcttcga
    27241 caacgcctac acggcccgtt gagaggacag gcggggcgca ttacctcccg tcgctccgcc
    27301 ttcttttttc cgctgtttcg ggcaggagca gcgcgcacga gcgccgactt cgcgcgcgtg
    27361 gcagggcgcg caccggcatc agccgccggt ccaccaggcc gcgaattcgt cgaagctgat
    27421 gctgccgtcg tcgtccccgt cggcgccctg gaacgcgagc tggcattctt ccctcgacag
    27481 ctcgtcgtcg agttctgcca tcagctggca gaactccgca aattcgatgc ggccgtcgct
    27541 gtcggcgtcg tactcctcga accgcgcgcg caattcatag agggcatcgg ggctcttggc
    27601 gctcatggat tctccctgtc caccggcgcc cattaaaacg cgggcctgcc gctgcgtaca
    27661 atatgcgcat gaaaggccca ggcggcaaac cggtccccgt gcgagtcgga cgcgagacca
    27721 agggtcgtgg tggcaagggc gtcaccgtga tcagcggcct caggctgtcg gtcgccgagc
    27781 tcgaggcgat cgccggtgag ctgaagcgcc ggtgcggctg cggcggcacc gtgaaggacg
    27841 gtcacatcga gatccagggc gatcatcgcg acacgatcgt cgcggcactg cgtgcgcgcg
    27901 gcttcgacgc aaagcgctcc ggcggctgag cggccacacg ccgcgaccgg ccgccgccgc
    27961 tgcctcagaa cagcgcccct tcgtcgtcct cgccgagcat ggctttgcgc accatcggaa
    28021 tgggcggccc gccgtacgag agaaactcgt cgtggaactg cttccatgcg ccgcggccac
    28081 cgcgtgtcga ggtccagtcc tcgcgcagct tgcggatcat cagcttgccg agcgtgtaat
    28141 tgaggtacgc cggatcgtag gtgccgcgcg ccgcctgctg gcgcgcgttg ccgggatcct
    28201 ggaaggcctg ctcgaggaac atgcgctcgg attccgccac cgtcatcccg cccgtgtgca
    28261 ggccgatcgc cgacaggtag cgcgcattgc gcagcagggc gttcgacagc tggccgacct
    28321 gggccgcggg atcgtccttg tgcaggccca tctcgaccat catctcttcg gtgtaatgcg
    28381 cccagccttc ggcgaacgcg tagcccacga acagcctgcc gaccagcgac tgcgactggt
    28441 tcgcatgcag gaaattcagg aagtgaccgg gccacacctc gtgcacggac gtgaacagca
    28501 ggtcggcttc gcccgggata tacgcgagct ggtcggcgcg gctccacttc ggatccggcg
    28561 gcgcgatgta ataggtcgcc ggcagcgatt tctcgtacgg ccccgggatc tcgatgtacg
    28621 cggagttcgc gcgattgtag ggaggcgatt ccgccacgag cgcctgctcc tcgctgggca
    28681 ccgacaccac cttgtggtcg atgacgaact ggcgcagctc gacgagctgg cgcgtggcac
    28741 cggcgaccgg cccgcccttg ggcttgtccg cggccaactt cgcgaagcac gccttgatcg
    28801 acttgcccgg ggtgaacttc tcgcacgccg tctgcagcga ggccaggttg cgctcgaggt
    28861 cggcgcgacc gatcttctcg agttcggcga gcggggtcgt cacctgctcc gtcgcagcga
    28921 gcatctgcgc gaatcgttcc gcgccgagcg cgaaatcctg ggtggcgccc gcgcgctgag
    28981 cctcgaagtg cttcgcgagg tcgcgcatcg catccgccgc agccttgttg gcggtcgcga
    29041 actcggcctg cagcgccgca tcgtcgaccg acgcgaagat cgcgggcacg tccgtatcga
    29101 agaagctggc gtagccgccg aaaccggaaa tcgcgcgctc gacgaaagtc agcggcatcg
    29161 gcaggcgcag gttctccttt atctgggccg cggcgccggg cacgcccttc gcataggtca
    29221 cgtaggcgcg catgcggtcg gcgagcggcg cgtaatcacg ggtgaggtag accgaagggc
    29281 cgagcaagcc caggtagtag gtggggttgg tgaacggcag ctcggcggtc tcctcccaga
    29341 acagggaccg atcgatgcgc gccagcaggt agtcgcgttc gaacaattgc gcggcatcga
    29401 gtccgagcgg accgatcgcc tgcgcggcat cgcgcgcagc gtgcaggcgc gcgatccgcg
    29461 ccgcgatgcc cgccttgctc cagtcgggca ggccgccatc gtattcgtgc cggcccgcat
    29521 ccacggcgaa cggaggatta tcgcgcagcg cgtcctcgat gaaggtatcg cggaacgtcg
    29581 tccatgcggc tggcgccgcc gctgccgccg ccctcatctc gtcggtgcgc gtgctgcagc
    29641 cgacggacag cgcggcaagt accgcgacca ggatccaggc agttccttgt ctgaccatgt
    29701 ggtgggccct ccgtggcgtc atcctacagt actcgcggca ttgacaatca cgcccggtcg
    29761 attacggtag ccccatgcga caccgctacg tcgtcggtgc tgtcatcgcg ttcgccgcga
    29821 tcagcgggct cgtctacctg ttctggccca agcccatcgc agagccgccc gtgctccctt
    29881 ccacggtgac cgacgcgcct ctgccgccgc cgatcgagca tccgttgccc gaagcgccgc
    29941 ccgacagcgc cgcaccaccc ctgcccgcac tcgccgacag cgacgcctac ctgctggatg
    30001 cactcggtga gctcatcggg gcgcagcccg caaacgacct ggttgtcgtc ccgggcatcg
    30061 cgcgccgcat cgtcgccacg gtggaccatc tcggacgcga caaactgccc gtgctcaccc
    30121 gcgccgtcgg acccatcccc ggcagcctcg ccgtggtgcg cgacggcgat cgaatcacgc
    30181 tcgccgaagc caacttcgcg cgctatgcac cctatgtcgc gctcgtcgag cgcctcgatg
    30241 tgagcaggct ggcggcgctg taccagcgca tctatccgct gctgcagcag gcctacggcg
    30301 aaatcggtcc ggaaggccgc tacttcaatg atcgcgtgat cgcggtcatc gacgacctgc
    30361 tcgcgacccc gcaggtcgca ggcccgatcg agctcgaaca gccgagcgtg catttccgtt
    30421 tcaaggaccc cgcgctcgag gcgcgctcct cgggacagaa actgctgctg cgcatgggcg
    30481 aggcccatgc cacggtggtg aaggaaaggc tgctcacgct gcgggcgttg atcgcccgtc
    30541 agtagagcag caggctctcg cgctcctcgc gccagcgcgc ctcgtccgcg gccgcgatcg
    30601 cttcgagatc gccgggagtc gccgccggat cgtccaccca ctcgcgcagc gccgtgccgc
    30661 cgttgatcag gtcgatcgcg agccggtcgc gctcgtactc gtaggcgaag tcacgccaca
    30721 gcggatattc ggggtagagc ccgcgtatcg ccttgaacgc caccgcgatc aatcgccatg
    30781 gcctgaaggc ctcgtgtagg taggccgtgt cgtcgacatg gacctgcagc gccgagcaca
    30841 gcttgccgac atgtttgtgg aaggtcggct cgaaccagca ggggcgcagg cggcagccgc
    30901 cgagccagcc cggcgcgatc cgctgcatct cgcgcagcac ggccgaggcg tcgatatccg
    30961 gcgccccgaa cagttcgagc ggccgagtag tgccgcgccc ttcggagagc gtggtgccct
    31021 cgagcatgac cgtgccggca tagcagcgcg ccatcgacag gttcggtgcg ttcgg
//
LOCUS       DAOQVY010000013        30910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_1722_length_30910_cov_5.438146, whole genome shotgun sequence.
ACCESSION   DAOQVY010000013 DAOQVY010000000
VERSION     DAOQVY010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 30910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30910
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1722_length_30910_cov_5.438146"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="PKN91_02375"
     CDS             complement(<1..147)
                     /locus_tag="PKN91_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009392033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS26946.1"
                     /translation="MAQYIYTMNRVSKVVPPKRTILRDISLSFFPGAKIGVLGLNGAG
                     KSTLL"
     gene            221..1162
                     /locus_tag="PKN91_02380"
     CDS             221..1162
                     /locus_tag="PKN91_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26947.1"
                     /translation="MAPVLVVMGDEIARYGFPAGHPFGVDRHAAFVRQFEARGLGARV
                     AIAPPRLAECAELLLFHTPGYVNFVHERSCTGQGCLDGGDTPAFRGVYEASAAVVGAT
                     LVAVGAIMGGAARRAFVPIAGLHHAARERAAGFCVFNDIGVAIEVLRREHGLARIAYI
                     DIDAHHGDGVYYAFEDDPAVIFADVHEDASTLYPGTGFASETGKGAAAGTKLNVPLPA
                     GADDAAFAAVWPGVLAFVEAARPEFIILQCGADSIAGDPITHMGFSASVHGRVAHDLC
                     LLADRLGHGRVLALGGGGYNRDNLAAAWNNVVEALAG"
     gene            1235..2491
                     /gene="glyA"
                     /locus_tag="PKN91_02385"
     CDS             1235..2491
                     /gene="glyA"
                     /locus_tag="PKN91_02385"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNS26948.1"
                     /translation="MFRTSMAIADFDPELAAAIANERRRQEEHIELIASENYASPRVL
                     EAQGSVLTNKYAEGYPGRRYYGGCEYVDIAEQLAIDRAKQLFGAAYANVQPHSGSQAN
                     AAVYLALCNPGDTILGMSLDHGGHLTHGAKVNFSGKLFHVVQYGIRPDNGEIDYDEVA
                     RLARTSKPRVIVAGFSAYSRVIDWSKFRAIADEVGAYLFVDMAHVAGLVAAGIYPNPV
                     PHADAVTTTTHKTLRGPRGGLILARENPELAKKFNSLVFPGTQGGPLMHVIAAKAVAL
                     LEALQPEFTVYQQQVLANARAMSARLMQRGYRIVSGGTDNHLFLMDLSDREITGKEAD
                     AALGRANITVNKNAVPNDPRPPMVTSGLRIGTPASTTRGFREAEVETVAGWIADVLDA
                     NGGAEAVERVRGEVLALCRRFPVYGP"
     gene            2513..3031
                     /gene="nrdR"
                     /locus_tag="PKN91_02390"
     CDS             2513..3031
                     /gene="nrdR"
                     /locus_tag="PKN91_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNS26949.1"
                     /translation="MHCPFCGHEETRVTDSRLAGEGRQIRRRRECLKCAERFTTFETA
                     ELVLPMVVKSDRSREPFDEQKMRHGIIKALEKRPVSQEAIEAAVTHICRKLLNLGERE
                     VPSRSIGELVMEELHQLDEVAYVRFASVYRSFQDVEAFRDEVEKLRRHARQRVDSNQL
                     PLLPGADGDPKK"
     gene            3028..4143
                     /gene="ribD"
                     /locus_tag="PKN91_02395"
     CDS             3028..4143
                     /gene="ribD"
                     /locus_tag="PKN91_02395"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNS26950.1"
                     /translation="MMVEFTAFDRQAMARALELAALGMESADPNPRVGCVIAQGTRIV
                     GEGWHVRAGEPHAEIVALAAAGASAAGATAYVTLEPCSHFGRTPPCADALIAARVARV
                     VFAVGDPNPRVDGGGAARLAAAGIDVASGLLEAQASALNVGFLKRMRSGRPWLRLKSA
                     ASLDGRTALASGASRWITGEAARADVHHWRARSSAIVTGVGTVLADDPRLDVRRPTPL
                     VRAPARVVIDSNLRTPPAARLFDAAGQVILLHATGDAAAAPLAARGARVERIASESGQ
                     ASGRVDLGAAMHRLGELAMNELWVEAGPTLAGALLEAGLVDEWLHYVAPKLLGPQARP
                     LAELAPLRQLEGAREFTIAEVAQLGADLRIRLLPGGK"
     gene            4140..4736
                     /locus_tag="PKN91_02400"
     CDS             4140..4736
                     /locus_tag="PKN91_02400"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNS26951.1"
                     /translation="MKVFTGIIQDVGTVRGVTPRGGDTEFVFAVDRLPLAGVRPGDSI
                     AVNGVCLTATRVGADEFAADVSRETLAVTTLAELASGTRVNLEPALRAGDALGGHLVS
                     GHVDGVAEVLAIEPDARSVRVTIGVPRQLARYIARKGSVAVDGVSLTVNGVDGEVFDV
                     NLVPHTQSVTTLGAWAPGRRVNLEVDQVARYVERLIGR"
     gene            4740..5840
                     /gene="ribBA"
                     /locus_tag="PKN91_02405"
     CDS             4740..5840
                     /gene="ribBA"
                     /locus_tag="PKN91_02405"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNS26952.1"
                     /translation="MELNTIDEILGDLRAGRMVLIMDDEDRENEGDLLMVAEKVRPED
                     INFMARYGRGLICLTLTAERCRQLSLPLMVSSTQRDLSTNFTLSIEAAEGVTTGISAY
                     DRAHTVRTAVRPDARAEDLRHPGHIFPIMSQPGGVLTRAGHTEAGCDLARLAGFEPAA
                     VIVEVMNDDGTMARRPQLEQFARHHGIKIGTIADLIRYRLRNERSVERIAERSVTTGF
                     GEFRLYAYEDHVQRDVHLALVRGEIGEGSVPLVRVHLADTLRDLLDVDLGAPRSWGLR
                     AAMARIAREGAGVVVVLRRNEPPRDVIAAIRDEEPPQAAAQGEVLRTFGVGAQILKDL
                     GVRRMQVLSAPKQMHGISAFGLEVEGYVDNDG"
     gene            5868..6335
                     /gene="ribH"
                     /locus_tag="PKN91_02410"
     CDS             5868..6335
                     /gene="ribH"
                     /locus_tag="PKN91_02410"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNS26953.1"
                     /translation="MPATHTGDLSAAHLSVAVVAARFNDFIVASLLRGAVEAWERQGG
                     AAERLVIARVPGAYELPVAARQCAASGRFAAVVALGCVIRGDTAHFDYVAGECARGLT
                     RVALDTGVPVMFGVLTVETVEQAVQRAAVRAGNKGGEAMLGAIEMANLLPRLA"
     gene            6332..6778
                     /gene="nusB"
                     /locus_tag="PKN91_02415"
     CDS             6332..6778
                     /gene="nusB"
                     /locus_tag="PKN91_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNS26954.1"
                     /translation="MTDKPVSPRAASHRRAVARKVAVQALYRWQVNAGPWQDLVQEFA
                     DAEDMPKADGEYFHELVREIAGHAERFDDLLARFTDRPPAELDPVEHAVLLVGIYELS
                     ARPDVPFRVVINEGVGLARRFGATDGHKFVNAVLDRAARELRPHEH"
     gene            6792..7748
                     /gene="thiL"
                     /locus_tag="PKN91_02420"
     CDS             6792..7748
                     /gene="thiL"
                     /locus_tag="PKN91_02420"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNS26955.1"
                     /translation="MPLSEFGLIDAYFRDVGARADVRTGVGDDGAVLMPPPGQDLVAV
                     VDTLVEGVHFPAGSDARSIGHRALAVNLSDIAAMGATPAWALLALTLPRADESWLAAF
                     TRGFGELARAHGVQLVGGDTTSGPLSVTVQVLGFVPQRQALLRTGARAGDIVHVTGTP
                     GEAACGLALLQGHEPRVDAPAAAIEGLRQRFLFPQPRADIGLRLVGIASACIDISDGL
                     AGDLAKLAQASHCGAEIDHALLPLSDALRACVGDDAAGQYALTGGDDYELCFTVPPAR
                     LGALAGLPCTAIGRIVEAPGLRLRRGDNVIEFSHSGFDHFRV"
     gene            7837..9156
                     /locus_tag="PKN91_02425"
     CDS             7837..9156
                     /locus_tag="PKN91_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNS26956.1"
                     /translation="MATPAPRANQHDKVPDKIGKYVIINEVGKGSTGTVYLSHDPYYG
                     RDVAIKVYSIDAGGDEERARISRKMFLSEAHMVGMLQHPHILPIYDAGEENGHCYIVT
                     EHVHGARTLAAYCKSDNLLRIDDVVEIIYKCAKALNYAHSRGVIHRDIKPSNIMLTQD
                     NDVRIIDFGIALVADSDISRIEGIAGSPSYMSPEQVQSLELTSRSDLYSLGAVMYEML
                     AGVRPFRAGNLAKLLHQIVYATPPPIHTLAPDVPEELEAVVATALQKSPDKRYKSGLD
                     LAAELTRVHQKLRQRHARIDQQEQFATLRRLKFFHEFSHAEIWEVLRASNWQDYAGSE
                     EIVKEGEMDDRFYVVVAGECVVERHGRRLGVLESGDCFGETSYVQGAKRTATVRANGP
                     VTVLKVSSTLLEQVSASCQLRFNRVFLRGMISRLQNAEQRVAAEAAP"
     gene            complement(9104..11200)
                     /locus_tag="PKN91_02430"
     CDS             complement(9104..11200)
                     /locus_tag="PKN91_02430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNS26957.1"
                     /translation="MHLPRSGLAFFVLSIGGMLHAGATALDPASIAKIPFVGDVQISA
                     DGKRIAYVVTHADLKKDGYQSDIWLIDGESAPRLLVGGAKDDARPRWSPDGSRLAFIS
                     DRAEKRQIHILDMRGGEAWPLTSEKAGAGAFAWSPDGKRIAYLARPAPANDGEEPKGA
                     GKARITERLYYRVDGAPGFLPEQRSQLWVVDVSPEHGAPLGPLWDGRTEPSEPAWTTD
                     GEALLFSALPDPNPPVAPVNSELFRVRVAAGSQAVALTVRDGPDNGPVAVARDGRVAW
                     TGFDDTVPRRSSTTTRLYAAPLGAGGLGAIANVTAGFDRNVGETSMSDVPPPTGGPGA
                     RARFDASGRTLYFIAADRGLAQLYRIAARGGEPQSISAQLRGDLREFSIASGGRIAAV
                     FGSATEPYEVWTADRPGGKWTRRTSHGHDAFAGTTLLPYEELWVESFDGTRIQAWLLK
                     PPGFDPARRHPLLLYIHGGPHAMYGEGFFHEFQMLAHAGLMVLIANPRGSTGYGEEFA
                     NSVQYRYPGDDYRDLMAVLDAVIARGHVDTARLGVGGGSGGGVLTSWTIAQTDRFAAA
                     LVERPVVDFASHTVVADLSSMFAQRWFHDLPWRSPQEYFERSPINFVDRVKTPVLVIQ
                     SEQDYRTPPDQGIGYYNALRMLDKPARLVLFPNSSHGLSRNGPPSQRIERLGIIRDWF
                     TERLLPPPAARHSAGD"
     gene            complement(11244..11837)
                     /locus_tag="PKN91_02435"
     CDS             complement(11244..11837)
                     /locus_tag="PKN91_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26958.1"
                     /translation="MKPILAKALKRFPQMIGHALTLQFYKARRDLGCVLKPIGFRRFF
                     SRHELLPLMVGPADAPGWISLPALLERATSGHRGWVLLKVDIEGGEYAALPAAVRHME
                     RVSALLIEFHALDREWDTFARCMRELAPRFHIAHIHGNNFDPCIRGTRVPQTIELTLV
                     NRALVPGEPQPSTRGFPSPGLDMPNTRKRPELQLTFD"
     gene            complement(11885..12022)
                     /locus_tag="PKN91_02440"
     CDS             complement(11885..12022)
                     /locus_tag="PKN91_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26959.1"
                     /translation="MELPGMMRVGRDHDGGYVLPRDVIERSHTLLSLGVNDDWSFEEA
                     A"
     gene            complement(12117..12920)
                     /gene="folE2"
                     /locus_tag="PKN91_02445"
     CDS             complement(12117..12920)
                     /gene="folE2"
                     /locus_tag="PKN91_02445"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNS26960.1"
                     /translation="MTPPIEDVQGRADSRRIPINKVGIKDIAHPVRVKDRAEGEQHTI
                     ATFNMYVNLPHNFKGTHMSRFVEILHAQREISVDSFRAMLAEMTRRLEADAGHIEMSF
                     PFFVMKAAPVSGVKSLMDYRATLIGEHRRGENTLWLKVVVPVTSLCPCSKKISERGAH
                     NQRSHVTIEVKLRTHMWIEELIEVAESEASSELYGILKRPDEKHVTEHAYDNPKFVED
                     LVRDVAVRLNRDDRIAAYTIEAENFESIHNHSAYALIERNKDDEAASGR"
     gene            complement(12917..14827)
                     /gene="dxs"
                     /locus_tag="PKN91_02450"
     CDS             complement(12917..14827)
                     /gene="dxs"
                     /locus_tag="PKN91_02450"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNS26961.1"
                     /translation="MSIPADTYPTLAAVARSPADLRTLPVARLGELADELRAYLISSV
                     STRGGHFAAGLGTVELTIALHYVFETPRDRLVWDVGHQAYPHKVLTGRGDRLHTIKQK
                     NGLAPFPSRAESEYDTFGVGHSSTSISAALGMAVAAALRGEQRSVVAVIGDGALTAGL
                     AWEAMLNAGASKADVIVVLNDNDMSISENVGALSNYLARVLSSRAYAQLRERGKRILE
                     QMPTMRELARRSEEHLKGMVLPGTLFEELGFTYHGPVDGHDLKALVPTLRNLRGRKGP
                     QFLHVVTRKGKGYAPAEADPITWHGPGPFDPASGTIFKEAARGPSYSQVFGEWLCDIA
                     ARNERIIGITPAMREGSGLVEYSKRFPGRYYDVAIAEQHAVTFAAGLACEGLRPVVAI
                     YSTFLQRAYDQVIHDVALQNLPVLFALDRAGLVGGDGVTHQGSFDFSFLRCIPNFVVM
                     APADENECRQMLYTATTLDSPVAVRYPRGTGSGVKIVQQMTALPIGKGEVRREGRSGL
                     LVLAWGALVEAAVAIGDRFDATVVNMRFVKPLDDELVLRLAARHRAVLTLEENVVAGG
                     AGSAVTELLAAEQLAVPVLSLGIPDRFIEHGSREDCLEAAGLDTPSLLAATERWWSLL
                     TAGPARVRIMAP"
     gene            complement(14856..15755)
                     /locus_tag="PKN91_02455"
     CDS             complement(14856..15755)
                     /locus_tag="PKN91_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNS26962.1"
                     /translation="MNDPSFEARIASLQARIEVVLDRALALPDAATTQLRAAMRYSTL
                     DGGKRLRPILVYASGAACGASPDLLDAPAAAVEMIHVYSLIHDDLPAMDDDDLRRGRA
                     TCHRAFGEPTALLAGDALQAHAFEVLATAGPATMPADVRLALVRELAAAIGTRGMAGG
                     QAIDLEAVGRTLDLATLENMHRRKTGALIRASVLLGAIAAGCTTGARHAALAHYGAEI
                     GLAFQIQDDVLDVEGSAASLGKRPGKDAAHAKPTYPSLVGLAEARRLAQAARDRAIGA
                     LAPLGDAGAELTQLAQYVVSREQ"
     gene            complement(15752..15997)
                     /locus_tag="PKN91_02460"
     CDS             complement(15752..15997)
                     /locus_tag="PKN91_02460"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNS26963.1"
                     /translation="MSKAAKAPDFERTLGELEQLVERLERGDLPLDEALKTFERGVAL
                     TRQCQEALKSAQAKVEILVSRGDATAIEAFEPDDDTE"
     gene            16153..16569
                     /locus_tag="PKN91_02465"
     CDS             16153..16569
                     /locus_tag="PKN91_02465"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNS26964.1"
                     /translation="MKALKGTKTEQNLKDAFAGESQANRRYLYFAAKADVEGYNDVSA
                     VFRSTAEGETGHAHGHLEYLEASGDPATGLPIGETKLNLKAAIAGETHEYTDMYPGMA
                     KAARDEGFDEIADWFETLAKAERSHANRFQKALDTL"
     gene            16566..17927
                     /locus_tag="PKN91_02470"
     CDS             16566..17927
                     /locus_tag="PKN91_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNS26965.1"
                     /translation="MTANRGSREGSLEAPTRHPIDWRSPAFHDEHALEKELERVFDIC
                     HGCRRCFALCNAFPVLFDAIDATDSGELEGVDKRVYWDVVDHCYLCDMCFLTKCPYVP
                     PHEFNIDFPHLMLRAKSVGNKKGRPRLRDKVLSATGVVGALAGIPVVAEIVNAANRSS
                     AGRALLEKTLGVDRNAPVPEYHSKSGRARANAQLRRSAAEGAPGAAVPTDDTRGRVAL
                     FVTCYGNRNETALPEDLVAVFAHNGIAVTVVGSERCCGMPKLELGDLETVARYKEANI
                     PRLKALVDAGHDIVAPIPSCVLMFKQELPLLFPGDADVKAVAEAMFDPFEYLMLRHKA
                     GLLRTDFVGTLGTVSYHVPCHLRVQNIGLKTRDVLRLVPGTTVDVIERCSGHNGTYAV
                     KREFRAASMRIAKPVISRVAGAAADHYSSDCPMAGHQIESGLDGAKAPEHPMRLLRTA
                     YGL"
     gene            17924..18505
                     /locus_tag="PKN91_02475"
     CDS             17924..18505
                     /locus_tag="PKN91_02475"
                     /inference="COORDINATES: protein motif:HMM:NF023432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HNS26966.1"
                     /translation="MSALAPGDLMSLEQYARERPAFRERVMAHKRNRRLAVGPNATWC
                     FEDRLTIQYQVQEMLRVERIFEPDGIADELGAYNPLIPDGGNWKVTLLIEFPDPVERA
                     QRLAALRGIEETCWVQVAGHERVLAIADEDLERENDEKTSSVHFLRFELQPAMIAALR
                     GGAALAAGIDHVDYSHEVQVPEATRRALLDDLA"
     gene            18608..20494
                     /gene="parE"
                     /locus_tag="PKN91_02480"
     CDS             18608..20494
                     /gene="parE"
                     /locus_tag="PKN91_02480"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNS26967.1"
                     /translation="MSQSYTASDIEVLSGLDPVRRRPGMYTDTSRPNHLAHEVIDNSV
                     DEALAGHCDEISVTLHKDGSLEVADNGRGMPVDIHPKEKVSGVELILTRLHAGGKFSD
                     SAYRFSGGLHGVGVSVVNALSKHLECWIRRGGKEYNISFKDGKVASKLEVIGTVGQKN
                     TGTTVRFWPDPQFFDNDRISVPQLRHVLKAKAVLCPGLRVRFANETSGEKDEWFFTGD
                     LGAYLDEEIGKVERLPAEPITGHSDGESDGVSFALAWVPDGGAAVAESYVNLIPTVDG
                     GTHVNGLRAGLVGAVREFCEFRNLVPRGVKLTAEDVWDKVCFVLSIKMRDPQFAGQMK
                     ERLGSRDAAQLVEGYAKDAIALWLNQHPEAGERIAQFAIANAQERLKAAQRVQRKRIA
                     SGPALPGKLADCTSQDPVHSELFLVEGDSAGGSAKQARDREFQAIMPLRGKILNTWEV
                     DAHDVSNSQEVHDISVALGVDPGSSDLADLRYHKVCILADADSDGQHIATLLCALFLR
                     HFRPLVSGGHVHIAMPPLFRIDAGKSVYYALDEAERDEILQRLATDKARARPVVTRFK
                     GLGEMNPSQLRETTMDPRTRRLVQLTVSAEDKADQLMDMLLAKKRAADRREWLEHKGN
                     LAQV"
     gene            20491..22746
                     /gene="parC"
                     /locus_tag="PKN91_02485"
     CDS             20491..22746
                     /gene="parC"
                     /locus_tag="PKN91_02485"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HNS26968.1"
                     /translation="MTMQDQELNFEGIEKQSLRAFTEKAYLDYSMYVILDRALPALGD
                     GLKPVQRRIIYAMSELGLSAGSKHKKSARTVGDVIGKFHPHGDSACYEAMVLMAQPFA
                     YRHPIVDGQGNWGSPDDPKSFAAMRYTEAKLTRYAEVLLRELGEGTVDWQPNFDGTLE
                     EPAMLPARLPNLLLNGTSGIAVGMATDVPPHNLREVAAACLHLLEEPEATTRALMKFV
                     KGPDLPTGAEIVSPRADLVEFYDKGVGSYKARAVYAIEDGDIVITRLPHQVSGSRVLE
                     QIAEQMRNKKLPMVEDLRDESDHENPTRLVIVPRSNRVDLEQLMAHLLATTDLERNYR
                     VNLNVIDLDGRPRVMGLRELLSGWLEFRIATVRRRLEWRLEKVNRRLHILEGLLAVYL
                     NLDEVIRIIRREDEPKPVLMKRFKLSEEQAEAILETKLRHLARLEEMKIREEQKALSD
                     ERDEIEATLKGKAKLRKLVADELRADAEKYGDERRSKIVEREAAQAIDAKELLANDPV
                     TVVLSAAGWVRAAKGHEIDPLSLSYKTGDEFQSAARGRSLQPAVFIDSTGRAYSLPAH
                     TLPSARGQGEPLSGRLAPPDGARFAGVLMGEPEDLWLLASDAGYGFTVRIRELLSDRK
                     AGKAVLTVPDRALVLPPVPVTSADALVAVVNSEGKLLVFPASDVPELAKGKGNKLFGI
                     SGKKAQSRAEVLTGIAVVPAGGSLVVHAGERRMTLTSGDLKDYRGERAQRGALLPRGW
                     RTVQRLSALAP"
     gene            complement(22754..24820)
                     /locus_tag="PKN91_02490"
     CDS             complement(22754..24820)
                     /locus_tag="PKN91_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNS26969.1"
                     /translation="MPAGGSILSDTTAVPVIVFAPSRDAVEAVNSHLRRAGHAVHCTW
                     IPELGDLGDALTQINPELLVCQPRAAGELSAVSAVRTQLTPEVPLVALIDNADEASIA
                     AAIAAGARDAVTLARPDRLRAVIERELRSYRLERALNTTLQSARDYRKQLEAVLERST
                     DAVVQVQEGIIVDANASWLNVFGIADAAALVGQPLMDLFEESSHTALKGALVACLQGR
                     WTDQVLKADALRHDGTVLSLEFVLSQGVFDGEPCVRLIVPARKRDERELASELADSVR
                     RDPGTGLLYRRPLLEAIAARTSTPARGGVRYLALARLDKFAELEKEIGPVASEQLLTE
                     FAGMLREHASPTDIAGRFSGTSFLLLLERGNERDVEAWAEQLVARTAKHVFHVGAKTL
                     GATCSVGLGVVPHGSPHIDDAVLDAVDACRRARHQGGGRVAMLDRADADSRVQAYDQV
                     WVRHIKAALMENRFRLVQQPVASLRGDDPQMFDVLVRMLDHQKKEVLPADFMAAAERN
                     DLLRNIDRWVVGASLSFAAQRRPGCLFVRLAKDSMLDPSFPGWLDGQRKASGAEPGRV
                     CFQVTEDVAAAYIKQAKALADAVKARGFRFAIERFGGGRDSHGLLAAMPVDFIKIDGA
                     LIQNLAGDIDLQQKVRALVDAASKRRIATIAERVEDANTMAVLWQLGVQYIQGYFVNA
                     PEDVHL"
     gene            complement(24832..25878)
                     /gene="epmB"
                     /locus_tag="PKN91_02495"
     CDS             complement(24832..25878)
                     /gene="epmB"
                     /locus_tag="PKN91_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HNS26970.1"
                     /translation="MITARLTGRQPLSWQKELAQAVTDPAQLLAALSLDTALLAAAQA
                     AAAGFRMRVPWSYVRRMRRGDPRDPLLRQVLPLGDELVDAPGYSADPLGERAANRVPG
                     LLHKYHGRALLITTGACAVHCRYCFRREFPYEAQVTDGRWSEALRAIAADRRVEELIL
                     SGGDPLSLSNERLAALTGQLTGIAHLARLRIHTRAPIVLPSRVDGGLAHWLAQLPWPV
                     VVVVHANHAQEIDAEVAAAFARLRAAGVTLLNQSVLLAGVNDDADALANLSTTLFAAG
                     ALPYYLHLLDRVRGASHFDVTEARGRELMRAISARLPGYLVPKLVREEAGAPGKTLIS
                     FDETSVLRDGRPSR"
     gene            25943..26512
                     /gene="efp"
                     /locus_tag="PKN91_02500"
     CDS             25943..26512
                     /gene="efp"
                     /locus_tag="PKN91_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNS26971.1"
                     /translation="MATYNTSEFRSGVKLLLDGDPYVVLENEFVKPGKGQAFNRVKVR
                     NLKTGRTIERTFKSGDTAEAADVVDIDMQYLYQDGDFYHFMVPDSFEQHTAGKTAVGD
                     AAQWLKDGTVCMVTLWNGVPLQVTPPPHIELKVVETDPGVRGDTATGGQKPAKLETGA
                     VVRVPLFINEGEVLRVDTRTGEYISRAKG"
     gene            complement(26549..27073)
                     /locus_tag="PKN91_02505"
     CDS             complement(26549..27073)
                     /locus_tag="PKN91_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNS26972.1"
                     /translation="MSFTGADYDFTDKARGYRELARAVTALLAHERDWIANTANATAL
                     LHDALPDLNWVGVYVPTAARTGVRELIVGPFQGKPACVRIAMGRGVCGTAAERRETIV
                     VPDVNAFDGHIACDTASRSEIVVPMLRTDRDDDAGLVGVLDLDSPRLARFDELDRAGL
                     EHIAAIIVAASDWP"
     gene            complement(27070..28047)
                     /gene="epmA"
                     /locus_tag="PKN91_02510"
     CDS             complement(27070..28047)
                     /gene="epmA"
                     /locus_tag="PKN91_02510"
                     /inference="COORDINATES: protein motif:HMM:NF006828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HNS26973.1"
                     /translation="MAAGPADFAPTATLAQLERRAAALASVREFFAVRAVMEVDPPAL
                     INAPVTDVNLHSARVQLPGPDPAATYALHTSPEYAMKRLLAAGSGDIYFLGHVFRGAE
                     RGRLHNPEFTLIEWYRRGFTLAQLMQEVAQLIERLCRRRFEAEHITYRAAFEREVGFD
                     PLAAPDEVLRDAAARLGLPEAALATCTRDELLDLIMGAAVGPRLGRGRLTFVQGYPAS
                     QAALARLDPRDARVALRFELYSEGMELANGFDELADAAEQRARFEADQRERQRRGLPI
                     GPLDERLLAALAAGLPPCAGVAVGFDRVLMLAEGAEHIDAVLPFTLECA"
     gene            complement(28054..28992)
                     /gene="asd"
                     /locus_tag="PKN91_02515"
     CDS             complement(28054..28992)
                     /gene="asd"
                     /locus_tag="PKN91_02515"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650253.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNS26974.1"
                     /translation="MSGIPGPGDLRDPGGAPTDSLGARVFVGMQYLLPQHGISRLVHR
                     IARVRTAWLKNLLIDAFVRQFRPDMADAVETDPRAYPSFNAFFTRALRSDSRPVDADE
                     TAIVSPVDGTVSECGPIRGDRLLQAKGHDYTLDALLAGDTALAKRFEGGQFATIYLAP
                     FNYHRIHMPLPGELTATAYVPGWLFSVNRATAAHVAGLFARNERIVCSFESPATPWAL
                     VLVGALNVGSMETVWHGEVTPRAPRTPAKLPNDASRAPLALPKGAEMARFNMGSTVIL
                     LLPAGRTQWTAGLEAGRVVRVGERIGTLVPPRLPAP"
     gene            complement(28989..29609)
                     /locus_tag="PKN91_02520"
     CDS             complement(28989..29609)
                     /locus_tag="PKN91_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNS26975.1"
                     /translation="MSAPKSWLSWSLLIVAAALTGAWIARATLTTEPAPELQSGTWLP
                     DARDLAMPTLVDSGGAPFTADSFAGHASLLFFGFTHCPDVCPNTLGLLAQVMRSTQAP
                     DLTVYLVSVDPERDTPEVLRAYLAGFDPQFIGLTGDPLDIKGFARTVGTAMIKVDLPG
                     DTYTMDHSATLYLLDDAGRLRAVFTPPFKAEELASDLAAAVAAVRG"
     gene            29608..30513
                     /gene="prmB"
                     /locus_tag="PKN91_02525"
     CDS             29608..30513
                     /gene="prmB"
                     /locus_tag="PKN91_02525"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: protein motif:HMM:TIGR03533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNS26976.1"
                     /translation="MTGLSHIRRANPTVRQVIANATRRLARAKLHYGHGTDNARDDAA
                     AIVWHVLGLPVPPRSRDYDRRVAARARQDIDALLERRIRERMPVVYLTQQTWFAGLSF
                     HVDERVLIPRSPIAELIERRFAPWIDASRVHDILDLGTGSGCIAIACAKAFPRAKVDA
                     SDISASALEVAAINVRRHRLGRRVRLVRSDHFAALGGRTYDIIVSNPPYVGAEELAAL
                     PPEYWHEPRAALESGDDGLDSLRVILAEAGRHLRPRGILVVEVGNSEVLARRRYPRVP
                     FTWLEFERGGGGVFVLTAEQVRDVR"
     gene            30503..>30910
                     /locus_tag="PKN91_02530"
     CDS             30503..>30910
                     /locus_tag="PKN91_02530"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNS26977.1"
                     /translation="MSGNTFGKLFTVTSFGESHGPALGCIVDGCPPGLELSAADLQPD
                     IDRRRTGTSQFTSQRREDDQVRILSGVFEGRTTGTPIGLLIENSDARSRDYEKIKDRF
                     RPGHADYAYLQKYGVRDYRGGGRSSARITVATVA"
BASE COUNT         4944 a        10460 c        10779 g         4727 t
ORIGIN      
        1 cagcagcgtg ctcttgcccg cgccgttcag cccgagcacg ccgatcttgg cgcccgggaa
       61 gaacgaaaga ctgatgtccc gcaggatcgt gcgcttcggt ggcacgacct tcgagacgcg
      121 attcatcgta tagatgtatt gcgccatgga ttccgatggg gtgggagagg gcggcgcgca
      181 ttatggggta gccggcgggc gtgtgctagg ctcccgcgcc gtggcaccgg tactcgtcgt
      241 gatgggcgat gagatcgccc gctacgggtt tcccgcaggt catccgttcg gcgtcgatcg
      301 gcatgccgca tttgtgcgcc agttcgaggc tcgcggcctc ggcgcccgcg tggcgatcgc
      361 gccgccgcgg ctcgcggagt gcgccgaact gctcctgttc catacgccgg gctacgtgaa
      421 cttcgtgcac gagcgctcgt gcacggggca gggctgcctc gacggcgggg acacgccggc
      481 gttccgggga gtttacgagg cctcggcagc ggtggtcggc gcaacgctgg tcgcagtggg
      541 cgcgatcatg ggcggcgcgg cgcgtcgggc attcgtgccg atcgcaggtc tgcaccacgc
      601 cgcgcgcgaa cgtgccgccg gcttctgcgt gttcaacgac atcggcgtcg cgatcgaggt
      661 gctgcgccgg gagcacggac tggcgcgcat tgcctacatc gacatcgacg cgcaccatgg
      721 cgacggcgtc tactacgcgt tcgaggacga tccggcggtc attttcgccg acgtgcacga
      781 ggatgccagc acgctgtatc ccggcaccgg cttcgcgagc gagaccggca agggtgcggc
      841 cgcgggcacc aaactcaacg ttccgttgcc ggccggcgcc gacgacgctg cgttcgcggc
      901 cgtctggccc ggcgtgctgg cgttcgtcga ggccgcgcgc cccgagttca tcatcctgca
      961 gtgtggcgca gacagcatcg caggcgaccc gatcacgcac atgggtttct ccgcgagcgt
     1021 ccacggccgg gtcgcgcacg acctgtgcct gctggccgac cgcctcgggc acggccgcgt
     1081 gttggcgctc ggcggcggcg gctacaatcg cgacaacctt gccgccgcat ggaacaacgt
     1141 cgtcgaagcg ctcgccggct gaccccgcgc cgaggcggga tcgggtagaa ttatcagttc
     1201 cccgggctgc ccgctgccgc gaaagtcacc gtccatgttc cgcacttcca tggccattgc
     1261 cgatttcgac cctgaacttg ccgccgccat cgccaacgag cgccgccgcc aggaagagca
     1321 catcgagctg atcgcgtccg agaactatgc cagcccgcgc gtactcgagg cgcagggctc
     1381 ggtgctgacc aacaagtacg ccgagggtta ccccgggcgg cgctactacg gcggctgcga
     1441 atacgtcgat atcgccgagc aactggcgat cgaccgcgcg aagcagctgt tcggcgctgc
     1501 ctatgcgaac gtgcagcccc attcgggctc gcaggcgaac gccgcggtgt atctggcgct
     1561 gtgcaatccc ggcgacacga tcctcggcat gagcctcgac cacggcggac atctgaccca
     1621 cggcgccaag gtgaacttct ccggcaagct gttccacgtg gtccagtacg gcatccgtcc
     1681 cgacaacggc gaaatcgatt acgacgaagt cgcgcggctc gcgcgtacca gcaagccgcg
     1741 cgtgatcgtc gccgggttct cggcctattc gcgcgtgatc gactggtcga agttccgcgc
     1801 gatcgccgac gaggtcggcg cgtacctgtt cgtcgacatg gcgcatgtcg cgggactcgt
     1861 ggccgcgggc atctacccga acccggtgcc gcatgcggac gccgtgacga ccaccaccca
     1921 caagacgctg cggggcccgc gcggtggcct gatcctcgcg cgcgagaatc cggaactcgc
     1981 caagaaattc aactcgctgg tattcccggg cacgcagggc gggccgctga tgcacgtgat
     2041 tgccgccaag gcggtggcgc tgctcgaggc gctgcagccc gaattcaccg tgtaccagca
     2101 gcaggtactt gccaacgcac gcgcgatgag cgcgcgcctc atgcagcgcg gctaccggat
     2161 cgtctccggc ggcaccgaca accacctgtt cctgatggac ctctccgaca gggagatcac
     2221 gggcaaggag gctgacgccg cgctcggccg cgccaacatc accgtcaaca agaacgccgt
     2281 gccgaacgat cccaggccgc cgatggtgac gagcgggctg cgcatcggca cgccggcatc
     2341 gacgacccgc ggcttcaggg aagccgaggt cgagacagtg gcaggctgga tcgccgacgt
     2401 gctcgatgcg aacggcggcg ccgaggcggt cgaacgcgtg cggggagagg tgctcgcgct
     2461 ctgccgccgc ttccccgtct acggtccctg atcccagcgc gcggggcgcg ccatgcactg
     2521 cccgttctgc ggccacgagg agacccgggt caccgattca cgtctcgccg gtgaagggcg
     2581 gcagatccgc cgccgtcgcg agtgcctgaa atgcgccgag cgcttcacga ccttcgagac
     2641 cgccgaactc gtgctgccga tggtcgtgaa gagcgatcgc agccgcgagc cgttcgacga
     2701 acagaaaatg cgccatggca tcatcaaggc gctggagaag cggcccgtat cgcaggaggc
     2761 gatcgaggct gcggtgaccc acatctgccg caagctgctc aatctcggcg aacgcgaggt
     2821 gccctcgcgc agcatcggcg agctggtgat ggaagaactg caccagctgg acgaggtggc
     2881 ttacgtgcgc ttcgcatccg tgtatcgcag cttccaggac gtcgaggcat tccgcgacga
     2941 ggtcgagaag ctgcgacgcc atgcgcgcca gcgcgtcgac agcaaccagc tgccgctgct
     3001 gccaggtgcg gacggcgacc cgaagaaatg atggtcgagt tcaccgcatt cgaccggcag
     3061 gccatggcgc gcgcgctcga gctcgccgcg ctcggcatgg agagcgccga cccgaatccg
     3121 cgcgtcggct gcgtgatcgc gcagggcacc cggatcgtcg gcgagggctg gcatgtgcgt
     3181 gccggcgaac cgcacgccga gatcgtggcg ctcgccgctg ccggcgcaag cgcggccggg
     3241 gccacggcct atgtcacgct cgagccgtgc agtcacttcg gccgcacgcc gccgtgtgcg
     3301 gatgcgctga tcgcggcacg cgtggcgcgg gtggtgttcg ccgtgggcga tccgaatccg
     3361 cgtgtggacg gcggcggcgc cgcgcgcctc gccgcggcgg gaatcgatgt cgcgagcggc
     3421 ctgctcgaag cgcaggcgag tgcgctcaac gtgggcttcc tcaagcgcat gcgcagcggc
     3481 cggccgtggc tgcgcctcaa gagcgcggcg agcctcgatg gccgcacggc gctcgcgagc
     3541 ggcgcgagcc gctggatcac cggtgaggct gcgcgcgcgg acgtgcatca ctggcgcgcc
     3601 cgcagttcgg cgatcgtgac cggcgtcggc acggtgctgg cggacgatcc gcgcctcgac
     3661 gtacgccggc cgacgccgct cgtgcgtgcg ccggcgcgcg tcgtaatcga ttcaaacctg
     3721 cgtacgccgc cggccgcgcg gctgttcgat gcggccgggc aggtgatcct gctgcatgcg
     3781 acgggcgatg cggccgcggc cccgctcgcg gcgcgcggcg cgcgcgtcga gcgcatcgcg
     3841 tccgaatcgg ggcaggcgtc ggggcgtgtc gatctcgggg cagcaatgca tcggctcggc
     3901 gagctcgcga tgaacgagct atgggtcgag gccggtccca ccctggccgg tgcgctgctc
     3961 gaggcagggc tggtcgatga atggctgcac tacgtggcgc cgaaactgct cgggccgcag
     4021 gcacggccgc tcgcggagct tgcgccgctg cggcagctcg agggcgcgcg cgagttcacg
     4081 atcgccgaag tggcgcagct gggcgcggac ctgcgcatcc gcctgctgcc gggaggtaag
     4141 tgaaggtgtt cacgggtatc atccaggacg tcggcacggt ccgtggcgtc acgccgcgcg
     4201 gcggcgacac cgaattcgtc ttcgcggtgg atcggctgcc actcgccgga gtgcgaccgg
     4261 gggacagcat tgccgtcaat ggcgtgtgcc tgaccgccac gcgggtcggc gcggacgagt
     4321 tcgcggcgga tgtgtcgcgc gagacgctcg cggtgaccac cctggcggag ctggcgagtg
     4381 gcacgcgtgt caatctcgaa ccggcgttgc gtgcgggtga tgccctcggc gggcacctgg
     4441 tgtcgggcca tgtcgacggt gtggccgagg tgctggcgat cgagcccgac gcgcgctccg
     4501 tgcgggtcac gatcggcgtg ccgcgccagc tcgcgcgcta cattgcccgc aagggctcgg
     4561 tggcggtcga cggcgtgagc ctgacggtga acggagtcga tggcgaggta ttcgacgtga
     4621 acctcgtgcc gcacacgcag tcggtgacga cgctcggcgc gtgggcgccc ggccggcgcg
     4681 taaacctcga agtggaccag gtcgcccgct acgtcgagcg actgatcgga aggtagacgg
     4741 tggaactgaa cacgatagac gagatcctcg gcgacctgcg cgccggccgc atggtgctca
     4801 tcatggacga cgaggaccgc gagaacgagg gcgacctgtt gatggtcgcc gagaaggtgc
     4861 gtcccgagga catcaatttc atggcgcgct acggccgcgg gctcatctgc ctcacgctga
     4921 cggccgagcg ctgtcgccag ctcagcctgc cgctgatggt gagcagtacc cagcgcgacc
     4981 tgagcaccaa tttcacgctg tcgatcgagg ccgcggaggg cgtgacgacc ggcatctcgg
     5041 cctatgaccg cgcgcacacg gtgcgcacgg cggtgcggcc cgacgcgcgc gcggaggacc
     5101 tgcgccaccc gggccacatt ttcccgatca tgtcgcagcc gggcggcgtg ctgacccgcg
     5161 ccggacacac ggaggccggt tgcgatctcg cgcgcctcgc cggattcgag ccggccgcgg
     5221 tgatcgtcga agtcatgaac gacgacggca cgatggcgcg gcgcccgcag ctcgagcagt
     5281 tcgcgcgcca ccacggcatc aagatcggca cgatcgcgga cctgatccgc taccggctgc
     5341 gcaacgagcg ctcggtggag cgcatcgccg agcgcagcgt cacgaccggg ttcggcgagt
     5401 tccgcctgta cgcctacgag gaccatgtgc agcgggacgt gcacctggcg ctggtgcgcg
     5461 gcgagatcgg cgagggctcc gtgccgctcg tacgcgtgca cctcgccgat acgctgcgcg
     5521 acctgctgga cgtcgatctc ggcgcgccgc gttcgtgggg cctgcgcgcg gcgatggcac
     5581 gcatcgccag ggagggtgcc ggcgtggtag tcgtgctgcg gcgtaatgag ccgccgcgcg
     5641 acgtgatcgc cgccatcagg gacgaggagc cgccccaggc agcggcccag ggcgaggtgc
     5701 tgcgcacttt cggcgtgggc gcacagatcc tgaaagacct cggtgtgcgg cgcatgcagg
     5761 tattgtcggc acccaagcag atgcacggca tctcggcctt cggcctcgag gtcgagggct
     5821 atgtcgacaa cgacggctga ggcgccaccc gcggctatac tgattgcatg cctgccaccc
     5881 acaccggtga cctgtccgcc gcgcacctgt cggtcgcggt cgtcgcggcg cgcttcaacg
     5941 acttcatcgt ggcgagcctg ctgcgcggcg cggtcgaagc ctgggagcgg cagggaggcg
     6001 ccgcggagcg cctggtgatc gcgcgcgtgc ccggcgccta cgagttgccg gtcgcggcgc
     6061 gccagtgcgc cgcgagcggc cggttcgcgg cggtggtggc gctcggttgc gtgatccgcg
     6121 gtgatacggc tcatttcgac tacgtcgccg gcgaatgcgc gcgcggcctg acgcgcgttg
     6181 cgctcgacac cggcgtgccg gtcatgtttg gcgtgctgac cgtcgagacc gtcgagcagg
     6241 ccgtgcagcg cgccgcggtc cgtgccggca acaagggcgg tgaggccatg ctcggcgcga
     6301 tcgagatggc caacctgctg ccgcgactcg catgaccgac aagcccgtca gcccgcgcgc
     6361 agcgagccac cgtcgagccg tcgcccgcaa ggtcgccgtg caggcgctct atcgctggca
     6421 ggtcaatgcc ggtccgtggc aggacctcgt gcaggaattc gccgatgccg aagacatgcc
     6481 gaaggcggat ggcgagtact tccatgagct ggtgcgcgag attgccggcc atgccgagcg
     6541 gttcgacgac ctgctcgcac gcttcaccga ccgcccgccg gcggagctcg atccggtgga
     6601 gcatgcggtg ctgctggtgg ggatctacga actcagcgcg cggccggacg tgccgttccg
     6661 cgtcgtgatc aacgagggcg tgggcctcgc gcgccgcttc ggcgccaccg acgggcacaa
     6721 gttcgtcaac gcggtgctcg accgtgcggc ccgcgaactg cgtccgcacg agcactgacg
     6781 cggatgccgc gatgccgctg tccgaattcg gcctgatcga cgcttacttc cgtgacgtgg
     6841 gagcgcgcgc cgacgtgcgc acgggtgtcg gcgacgacgg cgcggtactg atgccgccgc
     6901 ccggacagga cctcgtcgcc gtggtcgaca cgctggtcga gggcgtgcac ttcccggccg
     6961 gttcggacgc gcgctcgatc ggccaccgcg cgctcgccgt gaacctgagc gacatcgcgg
     7021 caatgggcgc aacccccgcc tgggcactcc tcgccctgac gctgccgcga gccgatgagt
     7081 cctggctcgc cgcgttcacc cggggcttcg gcgagctcgc acgcgcgcat ggcgtgcaac
     7141 tcgtcggagg agacacgacg tcgggaccgc tgtcggtcac cgttcaggtg ctcggcttcg
     7201 tgccgcagcg acaggcgctg ctgcgcaccg gcgcacgcgc gggcgacatc gtgcatgtca
     7261 ccggtacgcc cggcgaggcc gcgtgcgggc tggcattgct gcaggggcat gagccgcgag
     7321 tcgacgcgcc ggcggcggca atcgaggggc tgcgccagcg cttcctcttt ccgcagccgc
     7381 gcgccgacat cggcctgcgc ctcgtcggca tcgcgagcgc ctgcatcgat atttcggacg
     7441 ggctcgccgg cgatctcgcc aagctcgcgc aggcgagcca ctgcggcgcg gagatcgacc
     7501 acgcactgct gccgctgtcc gacgcgctgc gcgcctgcgt cggcgacgat gccgccggac
     7561 agtacgcact cactggcggc gacgattacg aactgtgctt cacggtgccg cccgcccgac
     7621 tcggtgccct cgcgggcctg ccctgtaccg cgatcggacg catcgtcgaa gcacccggcc
     7681 tgcggctgcg gcggggcgac aatgtgatcg agttctcgca ttccggcttc gatcacttca
     7741 gggtctgacg ccacgcgtca cagcccgcac gggccccggg cgatccgcga ggcgcatcac
     7801 agatcgcctc cggaggcgca cctatcctgc cgagcgatgg ccactcccgc ccccagagca
     7861 aatcagcacg acaaggttcc cgacaagatc ggcaagtacg tgatcatcaa cgaggtcggc
     7921 aagggcagta ccggcaccgt ttacctctcg cacgatccgt actatggccg cgacgtcgcg
     7981 atcaaggtct acagcatcga cgcgggcggt gacgaggagc gcgcgcgcat ctcgcgcaag
     8041 atgttcctgt ccgaagcgca catggtcggc atgctgcagc acccgcacat cctgccgatc
     8101 tacgacgccg gcgaggaaaa cggccactgc tacatcgtca ccgagcacgt gcacggcgcg
     8161 cgcacactcg cggcctactg caagtcagac aacctgctgc gcatcgacga tgtcgtcgag
     8221 atcatctaca agtgcgccaa ggcgctcaac tacgcgcact cgcgtggcgt gatccatcgc
     8281 gacatcaagc cgtcgaatat catgctgacg caggacaacg acgtacgcat catcgacttc
     8341 ggcatcgcgc tggtcgccga ttcggacatc tcgcgcatcg agggcatcgc cggcagcccc
     8401 tcgtacatgt cgcccgagca ggtgcagagc ctcgagctca cgagccgctc ggacctgtac
     8461 tcgctcggcg ccgtgatgta cgagatgctc gccggcgtgc ggccgttccg cgccggcaac
     8521 cttgccaagc tgctgcacca gatcgtgtat gcgacgcccc cgccgatcca cacgctcgcg
     8581 ccggatgtac cggaagagct cgaggccgtg gtcgcgacgg cgctgcagaa gagccccgac
     8641 aagcgctaca agagtggcct cgatctcgcg gccgaactca cccgcgtgca ccagaagctg
     8701 cgccagcgcc acgcgcgcat cgaccagcag gagcagttcg cgacactgcg acggctgaag
     8761 tttttccacg agttttcgca cgccgagatc tgggaagtgc tgcgcgcgag caattggcag
     8821 gattacgcag ggtcggaaga gatcgtcaag gaaggcgaga tggatgaccg gttctacgtc
     8881 gtcgtagccg gcgagtgcgt cgtagagcgc cacggacggc gcctcggcgt gctcgagagt
     8941 ggcgactgtt tcggcgaaac cagctatgtg cagggcgcca agcgcaccgc cacggtcagg
     9001 gccaacggcc cggtcacggt gctcaaggtc agctcgacgc tgctcgagca ggtctcggcg
     9061 tcctgccagc tgcgcttcaa ccgagtgttc ctgcgcggca tgatcagtcg cctgcagaat
     9121 gccgagcagc gggtggcggc agaagccgct ccgtaaacca gtcgcggatg atcccgagcc
     9181 gctcgatccg ctggctgggc gggccgttgc gtgacaggcc gtgcgatgaa ttagggaaca
     9241 gcacgagcct cgccggcttg tcgagcatcc tgagtgcgtt gtaatagccg atgccctggt
     9301 cgggtggcgt gcgatagtcc tgctcgctct ggatcacgag taccggtgtc ttcaccctgt
     9361 ccacgaagtt gatcggcgag cgctcgaagt attcctgcgg cgagcgccac ggcaggtcgt
     9421 ggaaccagcg ctgcgcgaac atgctgctga gatcggcgac cacggtgtgc gacgcgaaat
     9481 ccaccaccgg gcgttcgacc agcgcggccg cgaatcgatc cgtctgcgcg atcgtccagc
     9541 tcgtcagcac gccgccgcca ctgccgccgc cgacgccgag tcgcgcggta tcgacgtggc
     9601 cacgggcgat caccgcatcg agcaccgcca tcaggtcgcg atagtcgtcg ccgggatagc
     9661 ggtactggac gctgttcgcg aattcctcgc catagccggt gctgccgcgc gggttggcga
     9721 tcagcaccat caggccggca tgggcgagca tctggaactc gtggaagaag ccctcgccgt
     9781 acatcgcatg cgggccgccg tggatgtaca gcagcagcgg gtggcgccgc gccggatcga
     9841 agccgggcgg tttcagcagc cacgcctgga tgcgcgtgcc atcgaaggat tcgacccaca
     9901 attcctcgta gggcagcagc gtggtgccgg cgaacgcatc gtggccatgg ctcgtgcgcc
     9961 gggtccactt gccgccgggc cggtcggcag tccagacctc atacggctcc gtcgccgaac
    10021 cgaacaccgc cgcgatgcgc ccgccagagg cgatcgagaa ctcgcgcagg tcgccgcgca
    10081 gctgcgcgga gatcgattgc ggctcgccgc cacgcgcggc gatgcgatag agctgcgcga
    10141 ggccgcgatc ggccgcgatg aaatacagtg tccggccact cgcgtcgaac cgcgcccgcg
    10201 cccccggccc gccggtgggt ggcggcacat cgctcatcga agtctcgccg acattgcgat
    10261 cgaagccggc ggtgacgttg gcgatggcac cgagtccgcc agcaccgagc ggtgcggcat
    10321 acaggcgtgt cgtggtcgag ctgcgccgcg gtacggtatc gtcgaagccg gtccaggcga
    10381 cacggccgtc gcgcgcgacg gcgaccgggc cgttgtccgg cccgtcgcgc accgtgaggg
    10441 cgaccgcctg gctgccggcc gcgacccgta ccctgaacag ctcactgttg accggcgcca
    10501 ccggcggatt cggatcgggc agtgcgctga acagcagcgc ctcgccgtcc gtggtccatg
    10561 ccggctcgga gggttccgtg cggccgtccc acagcggacc gagcggcgcg ccgtgttcgg
    10621 gcgagacatc gaccacccac agctgcgagc gctgctcggg caggaagccc ggcgcgccgt
    10681 cgacgcggta atacaggcgt tcggtgatgc gcgccttgcc ggcgcccttc ggctcttcgc
    10741 cgtcgttcgc gggggcgggg cgcgcgaggt aggcgatgcg cttgccgtcg ggtgaccagg
    10801 caaaggcgcc ggctcccgct ttctcgctgg tcagcggcca ggcctcgccg ccgcgcatgt
    10861 cgaggatgtg gatctggcgt ttttcggcgc ggtcggaaat gaacgcgagc cgcgagccgt
    10921 ccggtgacca gcgcggccgc gcgtcgtcct tcgcgccgcc caccagcagg cgcggcgcgc
    10981 tctcgccgtc gatcagccag atatcgctct ggtagccgtc cttcttgagg tcggcatgcg
    11041 tcacgacata cgcgatgcgc ttgccgtcgg cgctgatctg cacgtcaccg acgaagggaa
    11101 tcttcgcgat cgatgccgga tcgagagcgg tggcgcccgc atggagcatg ccgccgatcg
    11161 acagaacgaa aaatgccagt ccgcttcttg gcaggtgcat ggcgtgcctt ccttgcgctg
    11221 agtctggcgc aatgatagcg gcttcagtcg aaggtgagct gcaattcagg ccgcttgcgc
    11281 gtgttgggca tgtcgaggcc cgggctcgga aagccccggg tcgatggttg cggctctccg
    11341 ggaaccagcg cgcggttcac gagcgtcaac tcgatggtct gcggcacgcg ggtgccccgg
    11401 atgcagggat cgaagttgtt gccgtggata tgggcgatgt ggaaccgcgg cgcgagctcc
    11461 cgcatgcatc gcgcgaatgt gtcccactcc cgatcgaggg cgtggaattc gatcagcaat
    11521 gcggacacac gctccatgtg ccggaccgcc gcggggagcg cggcgtattc gccaccttcg
    11581 atgtcgacct tcagcaggac ccaaccccga tggcccgacg tggcacgctc gagcagcgcg
    11641 ggcaatgaga tccagccagg ggcatccgcc gggcccacca tcaacggcaa caattcgtgc
    11701 ctggagaaga accgccggaa cccgatgggt ttcagcacgc agcccaggtc gcgtcgcgcc
    11761 ttgtagaact gaagcgtcag tgcgtggccg atcatctgcg gaaagcgctt cagcgccttc
    11821 gcgagaatgg gtttcatgcc cgtcgttccg tcgacgcagg tgacgcgcag ccccgcattg
    11881 atctctaggc ggcctcctcg aacgaccagt cgtcgttcac gccgaggctg agcagcgtgt
    11941 ggctgcgttc gatgacgtcg cgcggcagca cgtagccgcc atcgtggtcg cgcccgacac
    12001 gcatcatgcc cggcagttcc acgggcctgt agggggcaag cgagaccggc gtttggctgc
    12061 tgtccaggat ctgtaccccc gggcggatcc gtggccggcg cctttccgcg ccggcgctat
    12121 cgacccgacg cggcttcgtc gtccttgttg cgctcgatca gcgcgtaggc cgaatggttg
    12181 tggatcgatt cgaagttttc ggcctcgatg gtgtaggcag cgatccggtc gtcgcgattg
    12241 aggcgcactg cgacatcgcg caccaggtcc tcgacgaatt tcggattgtc gtaggcgtgc
    12301 tcggtcacgt gcttctcgtc ggggcgcttc agaatcccgt acagctcgct cgacgcctcc
    12361 gattcggcga cttcgatcag ttcctcgatc cacatgtgcg tgcgcagctt cacttcgatc
    12421 gtgacgtgcg agcgctggtt gtgcgcgccg cgttcggaga tcttcttcga gcacgggcac
    12481 aggctggtga ccggcacgac caccttgagc cacagcgtgt tctcgccgcg gcgatgttcg
    12541 ccgatcagcg tcgcgcggta gtccatcagg ctcttcacac cggacaccgg cgcggccttc
    12601 atgacgaaga acgggaagga catctcgatg tggccggcgt cggcttcgag ccggcgggtc
    12661 atctccgcga gcatcgcgcg gaaggagtcc accgaaatct cgcgctgcgc gtgcaggatc
    12721 tcgacgaagc gcgacatgtg cgtgcccttg aagttgtggg gcaggttcac gtacatgttg
    12781 aaggtggcga tcgtgtgctg ctcgccctcg gcgcggtcct tgacgcgcac cgggtgcgcg
    12841 atgtccttga tgccgacttt gttgatcgga atcctgcgac tgtcggcgcg tccctgcacg
    12901 tcctctattg gtggcgtcat ggggccatta tacggacgcg cgccggaccg gccgtcagta
    12961 gagaccacca gcgctcggtg gccgccagca ggctcggcgt atcgaggccc gctgcctcca
    13021 ggcagtcctc gcgcgaaccg tgctcgatga agcgatcggg aatgccgagc gacagcaccg
    13081 gcaccgcaag ctgctcggca gccagcaact cggtgaccgc agagccggcg ccgccagcca
    13141 ccacgttctc ctcgagcgtc agcacggcgc ggtgccgggc ggcaagtcgc agcaccagtt
    13201 cgtcgtcgag cggcttgacg aagcgcatgt tgaccacggt ggcgtcgaac cggtccccga
    13261 tcgcaaccgc cgcctcgacg agcgcgcccc acgcgagcac cagcaggccg ctgcgaccct
    13321 cgcgccgcac ttcacccttg ccgatcggca gtgcggtcat ctgctgcacg atcttcacgc
    13381 cagagcccgt gccgcgcgga taacgcaccg cgaccggcga gtcgagcgtc gtcgccgtgt
    13441 agagcatctg gcggcattcg ttctcgtcgg ccggcgccat cacgacgaag ttcggtatgc
    13501 agcgcaggaa cgagaaatcg aagctgccct ggtgcgtgac gccgtcgccg ccgaccaggc
    13561 cggcgcgatc gagcgcaaac aggaccggca ggttctgcag cgcgacgtcg tggatcacct
    13621 ggtcgtaggc gcgctgcagg aacgtcgagt agatcgcgac gaccggccgc agaccctcgc
    13681 aggcgagtcc cgcggcgaag gtcaccgcgt gctgctcggc gatcgcgaca tcgtagtagc
    13741 gtccggggaa gcgcttcgaa tactcgacga ggcccgagcc ctcgcgcatc gcgggcgtga
    13801 tgccgatgat gcgttcgttg cgcgcggcga tgtcgcacag ccactcgccg aacacctgcg
    13861 agtagctcgg gccgcgcgcc gcttccttga agatggtgcc gctcgccggg tcgaagggac
    13921 cgggtccgtg ccatgtgatc gggtcggcct cggccggcgc gtagcccttg cccttgcggg
    13981 tgacgacatg caggaactgc gggcccttgc ggccgcgcag gttgcgcagt gtcggcacga
    14041 gcgccttgag atcgtgtccg tcgacggggc cgtggtaggt gaagccgagt tcttcgaaca
    14101 gcgtgccggg cagcaccatg cccttcaggt gctcctcgga gcggcgcgcg agctcgcgca
    14161 tcgtcggcat ctgctcgagg atgcgcttgc cgcgctcgcg caactgcgcg taggcgcggc
    14221 tcgacagcac gcgcgccagg taattcgaca gtgcgccgac gttctcggag atcgacatgt
    14281 cgttgtcgtt cagcacgacg atcacgtccg ccttgctggc gccggcgttc agcatggcct
    14341 cccaggcgag tccggccgtg agtgcgccgt cgccgatgac cgccaccacg ctgcgctgct
    14401 cgccgcgcag cgccgcggcg acggccatgc cgagcgccgc gctgatcgag gtgctcgaat
    14461 ggccgacacc gaaagtgtcg tattcgctct cggcgcgcga cgggaacggc gcgaggccgt
    14521 tcttctgctt gatcgtgtgc agccggtcgc cgcgtcccgt cagcaccttg tggggatagg
    14581 cctggtggcc gacgtcccag acgaggcggt cgcgcggcgt ctcgaacacg tagtgcagcg
    14641 cgatggtcag ctcgaccgtg ccgaggcccg ccgcgaaatg cccgccgcgc gtgctcaccg
    14701 aactgatcag gtaggcacgc agttcgtccg caagctcgcc gaggcgggca acgggcaacg
    14761 tgcgcagatc ggcgggcgag cgcgcgacgg cggccagtgt cgggtaggtg tcggcgggaa
    14821 tgctcatggc ggatgcggaa attctacacg cgcgctcact gttcgcggga cacgacgtat
    14881 tgcgcgagct gcgtgagctc ggcgcccgcg tcgcccagcg gggcgagggc gccgatcgcg
    14941 cggtcgcgcg ccgcctgcgc gagacggcgg gcctccgcga ggcccaccag gctcgggtag
    15001 gtgggcttgg cgtgcgcggc gtccttcccc gggcgcttgc cgagtgatgc ggcgctgccc
    15061 tcgacgtcga ggacgtcatc ctggatctgg aaggcgaggc cgatttccgc gccatagtgc
    15121 gcgagcgccg cgtggcgcgc gccggtggtg cagcctgcgg cgattgcgcc gagcagcacg
    15181 ctcgcgcgga tcagcgcgcc ggtcttgcgc cggtgcatgt tctcgagagt cgcgagatcg
    15241 agcgtgcggc cgaccgcctc caggtcgatc gcctgcccgc cggccatgcc gcgcgtgccg
    15301 atcgccgcgg cgagttcgcg caccagcgca aggcgcacat cggccggcat cgttgcgggg
    15361 cccgccgtcg ccagcacttc gaaggcgtgt gcctgcagcg catctccggc gagcagtgcg
    15421 gtcggttcgc cgaacgcccg atgacaggtt gcgcgcccgc ggcgcaggtc atcgtcatcc
    15481 atcgccggca ggtcgtcgtg gatcaggctg tacacgtgga tcatctcgac ggcggcggcc
    15541 ggggcatcca gcaggtccgg cgatgcgccg cacgccgcgc cgctcgcgta gacgaggatc
    15601 ggtcgcaggc gcttgccgcc atcgagggtg ctgtagcgca tcgcagcccg cagttgcgtg
    15661 gtggcggcat ccggaagcgc cagggcgcga tcgagcacga cttcgatccg cgcctggagg
    15721 ctggcgatgc gcgcctcgaa ggacgggtcg ttcactcggt atcgtcgtcc ggctcgaacg
    15781 cctcgattgc cgtcgcatcg ccccgcgaca cgaggatctc gaccttggcc tgcgcagact
    15841 tcagcgcctc ctggcactgc cgggtgagcg ccacgccgcg ttcaaatgtc ttgagcgcct
    15901 cgtcgagcgg caggtcgccg cgctcgagac gttcgacgag ctgctcgagc tcgccgagcg
    15961 tccgctcgaa atccggcgcc ttggctgcct tgctcacctt gtcccccggc ccaaaaagtg
    16021 gcggcaacgt tacacatgcg gcccacgtcg ggcaacgcgt gctgcggggc gacacgaatt
    16081 gacgagcccg ggatagcgga ctacattgcg tcactctatt agactcagtc taacaaccgg
    16141 aggattcccc acatgaaagc tttgaagggc accaagaccg aacagaacct gaaggatgcg
    16201 tttgctgggg agtcacaggc caatcgccgc tacctctatt tcgcagccaa ggccgatgtc
    16261 gagggctaca acgacgtgtc ggcggtgttc cgctcgaccg ccgagggcga gacgggccac
    16321 gcgcacggcc acctcgaata cctcgaggcc tcgggcgacc cggccacggg cctgccgatc
    16381 ggcgaaacta agctgaacct caaggcagcc atcgccggcg agacccacga gtacaccgac
    16441 atgtaccccg gcatggcgaa ggcggcgcgc gacgaaggct tcgacgaaat cgcggactgg
    16501 ttcgagaccc tcgccaaggc cgagcgctcg cacgcgaatc gcttccagaa ggccctggac
    16561 acgctgtgac ggcgaaccgc ggctcccgcg aagggagcct cgaagcgccg acgcgccacc
    16621 cgatcgactg gcgcagtccg gcgttccatg acgaacacgc gctcgagaag gaactcgagc
    16681 gggtgttcga catctgccat ggctgcaggc gctgctttgc gctgtgcaac gcgttcccgg
    16741 tgctgttcga cgcgatcgac gccaccgaca gcggcgagct cgaaggcgtc gacaagcgtg
    16801 tgtactggga cgtcgtcgac cactgctacc tgtgcgacat gtgcttcctg acgaagtgcc
    16861 cctatgtgcc gccgcatgag ttcaacatcg acttcccgca cctgatgctg cgggcgaagt
    16921 cggtcggcaa caagaagggt cgcccgcgcc tgcgcgacaa ggtgctgtcg gccacagggg
    16981 tcgtcggggc gctcgcgggc atcccggtcg tcgccgagat cgtcaacgcg gccaatcgca
    17041 gcagtgcggg gcgcgcgctg ctcgagaaga ctctcggtgt cgatcgcaat gcgccggtcc
    17101 ccgaatacca ctcgaagtcc gggcgcgcac gcgccaatgc gcagctgcgt cgcagcgcgg
    17161 cggagggcgc cccgggtgcc gccgtgccca ccgacgatac ccgcggcagg gtcgcgctgt
    17221 tcgtcacctg ctacggcaat cgcaacgaga ctgcgctgcc ggaggatctc gtcgcggtgt
    17281 tcgcgcacaa tggcatcgcc gtcaccgtgg tgggcagcga gcgctgctgc ggcatgccga
    17341 agctcgaact cggtgacctc gagacggtgg cacgctacaa ggaggcgaac attccgcgcc
    17401 tgaaggcgct ggtcgacgcc ggccacgata tcgtcgcgcc gatcccgtcc tgtgtgctga
    17461 tgttcaagca ggagctgccg ctgctgttcc ccggcgacgc cgacgtgaag gcggtcgccg
    17521 aggcgatgtt cgatccgttc gagtacctga tgctgcgcca caaggcgggg ctgctgcgta
    17581 ccgatttcgt gggcacgctc ggtacggtca gctaccacgt gccgtgccac ctgcgcgtgc
    17641 agaacatcgg cctgaagacc cgcgacgtgc tgcgtctcgt gcccggcacc accgtcgatg
    17701 tcatcgagcg ctgctcgggc cacaacggca cctatgccgt gaagcgggag ttccgcgccg
    17761 cctcgatgcg gatcgcgaag cccgtgatca gccgcgttgc cggcgccgcc gccgatcact
    17821 actcgagcga ttgcccgatg gcgggccatc agatcgagag cggcctcgac ggcgccaagg
    17881 cgcccgaaca tccgatgcgc ctgctgcgta cggcgtacgg gctatgagcg cgctggctcc
    17941 cggcgacctc atgtcgctcg aacagtacgc ccgggagcgc ccggcgttcc gcgagcgcgt
    18001 gatggcgcac aagcgcaacc gccgcctcgc cgtcggcccg aacgccacct ggtgcttcga
    18061 ggatcgcctg acgatccagt accaggtgca ggagatgctg cgcgtcgagc gcatcttcga
    18121 acctgacggc atcgcggatg agctcggcgc ctacaacccg ctgattccgg acggcggcaa
    18181 ctggaaagtc acgctgttga tcgagtttcc ggatcctgtc gaacgcgcgc agcgcctcgc
    18241 ggcattgcgc ggcatcgagg aaacgtgctg ggtgcaggtc gccgggcacg agcgggtgct
    18301 ggcgattgcc gacgaagacc tcgagcgcga gaacgacgag aagacctcgt cggtgcactt
    18361 cctgcgcttc gaacttcagc ctgcgatgat cgcggcgctg cgcggcggcg ccgcgctcgc
    18421 cgcaggaatc gatcacgtcg actattcgca cgaagtgcag gtgccggaag ccacgcgccg
    18481 cgcgctgctg gacgatctgg cataggcgcc gggtcttcgg tatttcggtc gttgcggcgt
    18541 tcgccgcaga taaccgaaat accgaagacc cggcaccagg ttggctctgc taagattcgc
    18601 gcgcgcaatg agccagtcct acaccgcttc cgacatcgaa gtcctctccg gcctcgaccc
    18661 cgtgcgtcgc agaccgggca tgtacaccga cacgagccgc ccgaatcacc tggcgcacga
    18721 ggtcatcgac aacagcgtcg atgaggcgct tgccggccac tgcgacgaaa tctccgtcac
    18781 gctgcacaag gacggctccc tcgaggtcgc cgacaacggg cgcggcatgc ccgtcgacat
    18841 tcatccgaag gagaaggtga gcggcgtcga gctgatcctc acgcgcctgc acgccggcgg
    18901 aaagttctcc gacagcgcct accggttctc gggcggcctg cacggcgtgg gtgtctcggt
    18961 ggtcaatgcg ctgtcgaagc acctcgaatg ctggatccgc cgcggcggca aggaatacaa
    19021 catcagtttc aaggacggca aggtcgcctc gaagctcgag gtgatcggca ccgtcggcca
    19081 gaagaacacc ggcacgaccg tgcgcttctg gcccgatccg cagttcttcg acaacgacag
    19141 gatctcggtg ccgcagcttc ggcatgtgct gaaggcgaag gccgtgctgt gccccgggtt
    19201 gcgggtgcga ttcgcgaacg agacgagcgg cgaaaaggac gaatggttct tcaccggcga
    19261 cctcggcgcc tacctcgacg aggagatcgg caaggtcgag cgcctgccgg ccgaaccgat
    19321 caccggccac agcgacggcg agtccgacgg cgtgtcgttc gcgctcgcgt gggtgccgga
    19381 tggcggcgcg gcggtcgcgg agagctacgt gaacctcatc ccgaccgtgg acggcggcac
    19441 gcacgtcaac ggcctgcgcg cggggctggt cggcgccgtg cgcgagttct gtgaattccg
    19501 caacctcgtg ccgcgcggcg tgaagctcac cgccgaggac gtctgggaca aggtctgctt
    19561 cgtgctctcg atcaagatgc gcgatccgca gttcgccggc cagatgaaag aacggctcgg
    19621 atcgcgcgac gccgcccagc tggtcgaggg ctacgccaag gacgcgatcg cgctctggct
    19681 caaccagcac ccggaagcgg gcgagcgcat cgcgcagttc gcgatcgcca acgcgcagga
    19741 gcgcctgaag gccgcgcagc gcgtgcagag aaaacgcatc gcgagcgggc cggcgctgcc
    19801 gggcaagctg gccgactgca cctcgcagga cccggtgcat tccgaactgt tcctcgtcga
    19861 gggcgactcc gccggcggct ccgccaagca ggcgcgggat cgggaattcc aggcgatcat
    19921 gccgctgcgc ggcaagatcc tgaacacctg ggaggtcgac gcgcacgatg tcagcaattc
    19981 gcaggaagtg cacgacatca gcgtggctct cggggtcgat cccggctcga gcgacctcgc
    20041 ggacctgcgc taccacaagg tctgcatcct cgccgacgct gattcggacg gacagcacat
    20101 cgcgacgctg ctgtgcgcgc tgttcctgcg gcatttccgg ccgctggtca gcggaggcca
    20161 cgtgcacatc gcgatgccgc cgctctttcg catcgacgcc ggcaagagcg tctactacgc
    20221 actcgacgag gccgagcgcg acgagatcct gcagcgcctc gccacggaca aggcccgcgc
    20281 ccggccggtg gtcacgcgct tcaagggcct cggcgagatg aatccctcgc agctgcgcga
    20341 gaccaccatg gatccgcgca cgcgccggct cgtacagctc acggtcagcg ccgaagacaa
    20401 ggcggaccag ttgatggaca tgctgctcgc gaagaagcgc gccgccgacc ggcgcgagtg
    20461 gctcgagcac aagggcaacc tggcgcaggt atgacgatgc aggaccagga gctcaacttc
    20521 gagggcatcg agaagcagtc gttgcgcgcc ttcaccgaga aggcctacct cgactactcg
    20581 atgtacgtga ttctcgaccg cgcgctgccc gcgctcggcg acggcctcaa gccggtccag
    20641 cgccgcatca tctatgcgat gagcgagctg ggcctctcgg ccggctcgaa gcacaagaaa
    20701 tcggcgcgca cggtcggcga cgtgatcggc aagttccatc cgcacggcga cagcgcctgc
    20761 tacgaggcga tggtgctgat ggcgcagccg ttcgcctatc gccacccgat cgtcgacggc
    20821 cagggcaact ggggctcgcc cgacgatccg aagtccttcg cggcgatgcg ctataccgag
    20881 gcgaagctca cgcgctacgc cgaagtgctg ctgcgcgagc tcggcgaggg tacggtcgac
    20941 tggcagccga atttcgacgg caccctcgaa gagccggcga tgctgcccgc gcgactgccc
    21001 aacctgctgc tgaacggtac ttcgggcatc gcggtcggca tggcgaccga cgtgccgccg
    21061 cacaacctgc gcgaagtcgc ggccgcgtgc ctgcacctgc tggaggagcc ggaggcgacc
    21121 acgcgcgcac tgatgaagtt cgtcaagggt ccggacctgc ccaccggcgc ggagatcgtt
    21181 tccccgcgtg ccgatctcgt cgagttctac gacaagggcg tcggcagcta caaggcgcgc
    21241 gcggtatacg cgatcgagga tggcgacatc gtcatcacgc gcctgccgca ccaggtgtcc
    21301 ggcagtcgcg tgctcgagca gatcgccgag cagatgcgca acaagaagct gccgatggtc
    21361 gaggacctgc gcgacgagtc ggaccacgag aacccgaccc gcctcgtgat cgtgccgcgc
    21421 tcgaaccgcg tcgacctcga acagttgatg gcgcacctgc tggcgaccac cgacctcgag
    21481 cgtaattacc gcgtgaacct caacgtcatc gacctcgatg gccgcccgcg cgtgatgggc
    21541 ctgcgggagc tgctgtcggg ctggctcgag ttccgcatcg ccacggtccg gcggcgcctc
    21601 gaatggcggc tcgagaaggt caaccggcgg ttgcacatcc tcgagggcct gcttgcggtc
    21661 tatctcaatc tcgacgaggt gatccgcatc atccgtcgcg aggacgagcc gaagccggtg
    21721 ctgatgaagc gcttcaagct gtcggaggag caggccgagg cgatcctcga aaccaagctg
    21781 cgccacctcg ccagactcga ggaaatgaag atccgcgagg agcagaaggc gctgtccgac
    21841 gagcgcgacg aaatcgaggc cacgctcaag ggcaaggcga agctgcgcaa gctcgtcgcg
    21901 gacgagctgc gtgccgacgc cgagaagtac ggcgacgagc gccgctcgaa gatcgtcgag
    21961 cgcgaagccg cgcaggcgat cgacgcgaag gaactgctgg cgaacgatcc ggtcacggtc
    22021 gtgctgtcgg ccgcgggctg ggtgcgcgcc gcaaaaggcc atgaaatcga tccgctgtcg
    22081 ctgtcgtaca agaccggcga cgaatttcag tcggccgcgc gcgggcgcag cctgcagccg
    22141 gcggtgttca tcgacagcac gggccgcgcc tacagcctgc cggcgcatac cctgccgtcg
    22201 gcccgcggcc agggcgagcc cttgtcgggg cgcctcgcgc cgcccgatgg cgccaggttc
    22261 gccggcgtgc tgatgggcga acccgaggac ctgtggctgc tcgcatcgga tgccggctac
    22321 ggattcacgg tgcggatcag ggagctgctg tcggaccgca aggccggcaa ggccgtgttg
    22381 acggtgcccg acagggcgct ggtgttgcca ccggtgccgg tgacgtcggc cgatgcattg
    22441 gtcgcggtgg tgaacagcga gggcaagctg ctcgtgttcc cggcgtccga tgtgcccgaa
    22501 ctcgcgaagg gcaagggcaa caagctgttc ggcatttctg gcaagaaggc ccagtcgcgc
    22561 gcagaagtgc tgacgggcat cgccgtcgtg cccgccggcg gctcgctggt cgtgcatgcc
    22621 ggcgagcgtc ggatgacgct cacgagcggc gatctcaagg actatcgcgg cgagcgcgcc
    22681 cagcgcggtg cgctgctgcc gcgcggctgg cgcaccgtgc agcgcctctc ggcgctggcg
    22741 ccctgatcgc ccctcagagg tgcacgtcct cgggcgcgtt gacgaagtag ccctggatgt
    22801 actgcacgcc gagctgccac agcaccgcca tcgtgttcgc gtcctcgacg cgctcggcga
    22861 tcgtggcgat cctgcgcttg ctggccgcgt cgaccagcgc gcgcaccttc tgctgcaggt
    22921 cgatgtcgcc cgccaggttc tggatcagcg cgccatcgat cttgatgaag tcgaccggca
    22981 tcgcggcgag cagaccgtgc gaatcgcgcc cgccgccgaa gcgctcgatc gcgaagcgga
    23041 agccgcgcgc cttgaccgca tcggcaaggg ccttggcctg cttgatgtag gccgccgcca
    23101 catcttcggt gacctggaag cacacgcgcc cgggttcggc gccgctcgcc ttgcgctggc
    23161 catcgagcca gccggggaag gacggatcga gcatgctgtc cttcgcgagg cgcacgaaca
    23221 ggcagcccgg gcgccgctgc gccgcgaacg acagtgatgc gccgaccacc cagcgatcga
    23281 tgttgcgcag caggtcgttg cgctccgcgg cagccatgaa atcggccggc aggacttcct
    23341 tcttctggtg atcgagcata cgtaccagta cgtcgaacat ctgcggatcg tcgccgcgca
    23401 gcgaggcgac cggctgctgc accagccgga agcggttctc catcagcgcc gccttgatgt
    23461 gcctgaccca gacctggtcg taggcctgca cacgcgagtc cgcgtcggcg cgatcgagca
    23521 tcgcgacgcg cccgccgccc tggtggcggg cgcggcggca ggcatcgacc gcatcgagga
    23581 cggcgtcgtc gatgtgcggg ctgccgtgcg ggacgacacc cagcccgacc gagcaggtcg
    23641 cgccgagggt cttggcgccg acgtggaaca cgtgttttgc cgtgcgcgcg acgagctgct
    23701 cggcccaggc ttcgacgtca cgctcgttgc cgcgctcgag cagcaacaga aaggacgtgc
    23761 cgctgaaacg gccggcgata tcggtcgggc tggcgtgctc gcgcagcatg ccggcgaatt
    23821 cggtcagcaa ctgctcgctc gcaaccggcc cgatctcctt ttcgagctcc gcgaacttgt
    23881 cgagccgcgc cagtgcgaga tagcgcacgc cgccgcgcgc aggcgtcgag gtacgcgcgg
    23941 cgatcgcctc gagcagcggg cgccgataca gcaggcccgt gccgggatcg cgccgcacgg
    24001 agtcggcgag ttcgctggcg agctcgcgct cgtctcgctt gcgcgccggc acgatcagcc
    24061 ggacgcaggg ctcgccatcg aagaccccct gcgacagcac gaactcgagc gacaggacgg
    24121 tcccgtcgtg ccgcagcgcg tcggccttga gcacttggtc ggtccagcgt ccctgcaggc
    24181 aggcgaccag tgcgcccttg agcgcggtgt gcgaggactc ctcgaacagg tccatcagcg
    24241 gctggccgac gagcgcggcc gcgtcggcga tgccgaagac gttgagccag gaggcgttgg
    24301 cgtcgacgat gatgccctcc tgcacctgca ccaccgcgtc ggtgcttcgt tccagcaccg
    24361 cctcgagctg cttgcggtaa tcccgggcgg actgcagcgt cgtgttgagc gcacgctcga
    24421 gccgatagct gcgcagctcg cgctcgatga cggcgcgcag gcggtcgggg cgggccagcg
    24481 tgacggcatc gcgtgcgccg gcggcgatcg cggccgcgat cgacgcttcg tccgcattgt
    24541 cgatcagcgc gaccagcggc acttcgggcg tcagttgcgt gcgcacggcc gacaccgccg
    24601 acagctcgcc cgcggcgcgt ggctggcaga cgagcagctc ggggttgatc tgcgtaagcg
    24661 catcgcccag gtcgccgagt tcggggatcc aggtgcaatg cacggcgtga cctgcgcggc
    24721 gcaggtgcga attgacggcc tcgaccgcgt cgcgcgaggg cgcgaacacg atgaccggta
    24781 cggcggtcgt gtcagacaga atcgatcctc cagcaggcaa gcaaacgaag cttaccgtga
    24841 aggccgcccg tcgcgcagga ccgacgtctc gtcgaacgaa atcagtgtct ttcccggggc
    24901 gccggcttct tcgcgcacca gcttcggcac gaggtagccc ggcaggcggg cactgatcgc
    24961 ccgcatcagt tcccggccac gcgcttccgt gacatcgaaa tgtgatgcgc ctcgcacccg
    25021 atcgagcagg tgcaggtagt agggcagggc gccggcggca aacagcgtcg tcgacaggtt
    25081 cgcgagcgcg tccgcgtcgt cgttcacccc ggcgagcagc acggactggt tgagcagcgt
    25141 cacgccggcc gcgcgcaggc gcgcaaacgc ggcagcgact tcagcgtcga tctcctgcgc
    25201 atgattggcg tgcacgacga cgacgacggg ccagggcagc tgcgcgagcc agtgcgcgag
    25261 gccgccgtcc acgcgcgagg gcagcacgat cggcgcgcgg gtatggatgc gcaggcgcgc
    25321 gaggtgcgca atgccggtca gctggccagt gagcgccgca agccgctcgt tcgacaggga
    25381 gagcggatcg ccgccgctca ggatgagctc ctccaccctg cggtcggcgg cgatggcccg
    25441 cagcgcctcg ctccagcggc cgtccgtgac ctgtgcctcg tacgggaatt cgcggcggaa
    25501 gcagtagcga caatgcacag cgcaggcacc ggtcgtgatg agcagcgccc ggccgtggta
    25561 cttgtgcagc aggcccggca cgcgattggc ggcgcgctcg ccgagcggat cggcggaata
    25621 tcccggtgca tcgacgagtt cgtcgccgag cggcaggacc tgccgcagca gcgggtcgcg
    25681 cggatcaccg cggcgcatgc gccgcacata ggaccagggc acgcgcatgc gaaagcccgc
    25741 ggcggcggcc tgcgcggcgg cgagcagcgc ggtgtcgagc gacagtgccg ccagcagctg
    25801 cgccgggtcg gtgacggcct gcgcgagctc cttctgccag gaaagcggct ggcggccggt
    25861 aagtcttgcc gttatcattt gcgccctttt tttgggcctc cgagcggggg ccgcattctg
    25921 ggccccggcc cgagagaaat aaatggccac ctacaacacc agcgaattcc ggtccggcgt
    25981 caagctgctg ctcgatggcg acccctatgt cgtgctcgag aacgagttcg tcaagccggg
    26041 caaggggcag gcgttcaacc gcgtgaaagt ccgcaacctg aagaccggcc gcaccatcga
    26101 gcgcacgttc aagtcgggtg atacggccga ggccgccgac gtcgtcgaca tcgacatgca
    26161 gtatctctac caggacggcg acttctacca cttcatggtg cccgacagct tcgagcagca
    26221 caccgcgggc aagaccgcgg tgggcgatgc cgcgcagtgg ctcaaggacg gtaccgtctg
    26281 catggtgacc ctgtggaacg gtgtgccgct gcaggtcacg ccgccgccgc acatcgaact
    26341 gaaagtcgtc gagaccgatc cgggcgtacg cggcgacacc gcgaccggtg gtcagaagcc
    26401 cgccaagctc gagaccggtg cggtcgtgcg cgtgccgctg ttcatcaacg aaggcgaagt
    26461 cctgcgcgtc gatacgcgca cgggcgagta catctcgcgc gccaagggct gagtccgcgc
    26521 ccgcacgggc cggcggcagc ggcgatcgct agggccagtc gctcgccgcg acgatgattg
    26581 ccgcgatgtg ctcgaggccc gcgcggtcga gctcatcgaa gcgcgcgagg cgcgggctgt
    26641 cgaggtcgag cacgcccacg aggccggcat catcgtctcg atccgtgcgc agcatcggca
    26701 cgacgatttc cgatcgcgac gctgtatcgc aggcgatgtg cccgtcaaag gcattcacgt
    26761 ccgggacgac gatcgtctcg cggcgctcgg ccgccgtgcc gcacaccccg cgacccattg
    26821 cgatgcgcac gcaggccggc ttgccctgga agggacccac gatcagttcg cgcacgcccg
    26881 tgcgagccgc ggtggggaca tagacaccca cccagttgag gtccggcagg gcatcatgca
    26941 gcagcgccgt ggcgttcgcc gtgttggcga tccagtcgcg ctcgtgggcg agcagcgcgg
    27001 tgaccgcgcg cgcgagttcc cggtagccgc gcgccttgtc cgtgaagtcg taatccgcgc
    27061 ccgtgaagct catgcgcact ccagtgtaaa cggcagcacc gcatcgatgt gttcggcccc
    27121 ttcggcgagc atcagcaccc gatcgaagcc gaccgcgacc ccggcgcagg gcggcaggcc
    27181 cgcggcgagc gccgcaagca ggcgctcgtc gagcggcccg ataggcagac cgcgtcgttg
    27241 ccgctcgcgc tggtcggcct cgaagcgcgc gcgttgctcc gcggcatcgg cgagttcgtc
    27301 gaagccgttc gccagttcca tgccctcgga gtacagctcg aagcgcaggg cgacgcgcgc
    27361 atcgcgcgga tcgaggcgcg cgagtgccgc ctgcgaggcc ggatagccct gcacgaacgt
    27421 cagtctgccg cggccgagtc gcggtcccac cgcggcaccc atgatgaggt cgagcagttc
    27481 atcgcgcgtg caggtggcga gcgcagcctc cggcagaccc agtcgggccg ccgcgtcgcg
    27541 cagtacctcg tccggcgccg ccagcgggtc gaagccgact tcccgctcga aggctgcgcg
    27601 ataagtgatg tgctcggctt cgaaccggcg tcggcacaac cgctcgatga gctgcgcgac
    27661 ttcctgcatc agctgcgcga gcgtgaaacc gcgccgatac cactcgatga gcgtgaattc
    27721 gggattgtgc agccggccgc gctcggcgcc gcgaaacacg tggccgagaa agtagatgtc
    27781 gccactgccg gccgcgagca ggcgcttcat cgcgtactcg ggcgaggtgt gcagggcgta
    27841 ggtcgccgcc gggtccgggc ccggcagctg cacgcgtgcc gaatgcaggt tcacgtcggt
    27901 caccggtgcg ttgatcagcg ccggcggatc gacttccatg acggcgcgca ccgcgaagaa
    27961 ttcccgcacg ctcgccagcg cggcagcacg gcgttcgagc tgtgcgagcg tggcggtcgg
    28021 cgcgaaatcg gcgggacccg cggccatggc cgctcagggc gcgggcaggc ggggcgggac
    28081 gagggtgccg atgcgctcac ccacccgtac cacacgcccg gcctcgaggc ctgcagtcca
    28141 ctgtgtgcgc ccggccggca gcagcaggat caccgtggag cccatgttga agcgggccat
    28201 ttcggcgccc ttcggcaggg cgagcggcgc gcggctcgcg tcgttcggca gcttcgcggg
    28261 cgtgcgcggc gcgcgcggcg tgacctcgcc atgccacacc gtctccatgc tgccgacgtt
    28321 cagtgcgccg acgaggacca gggcccaggg tgtggcgggt gactcgaagc tgcacacgat
    28381 gcgctcattg cgtgcgaaca gcccggcgac atgcgccgcc gtggcgcgat tgacgctgaa
    28441 cagccagccc ggcacatagg cggtcgccgt gagttcgccg ggcagcggca tgtggatgcg
    28501 gtgatagttg aagggcgcga ggtagatcgt cgcgaactgg ccgccctcga accgcttcgc
    28561 gagcgccgtg tctcccgcga gcagtgcgtc gagggtgtaa tcgtggccct tcgcctgcag
    28621 caggcgatcg ccacggatcg ggccgcattc gctcaccgtg ccatcgaccg gcgagacgat
    28681 ggcggtctcg tcggcgtcga ccggccggct gtcgctgcgc agcgcgcggg tgaagaatgc
    28741 attgaagctc gggtaggcgc gcgggtcggt ttcgacggcg tccgccatat cgggcctgaa
    28801 ctggcgcacg aaggcatcga tcagcaggtt cttgagccac gcggtgcgca cccgtgcgat
    28861 ccggtgcacc aggcgcgaga tgccgtgttg cggcagcagg tactgcatgc cgacgaacac
    28921 gcgcgcgccg aggctgtcag tcggggcccc gccgggatcg cgcagatccc cgggaccggg
    28981 tatgccgctc atccacggac cgccgccacg gcggcggcga gatcgctggc gagttcctcg
    29041 gccttgaaag gcggcgtgaa cacggcgcgc agccgcccgg cgtcgtcgag caggtagagc
    29101 gtcgccgagt gatccatcgt ataggtgtcg ccgggcaggt cgaccttgat catggccgtg
    29161 ccgaccgtgc gcgcgaagcc cttgatatcg agcgggtcac ccgtaagacc gataaactgc
    29221 ggatcgaaac ccgcgagata ggcccgcagg acttcgggtg tgtcgcgttc cggatcgacc
    29281 gagacgaggt agacggtgag gtccggtgcc tgcgtgctgc gcatgacctg cgccagcagc
    29341 ccgagcgtgt tcgggcacac gtccgggcag tgggtgaagc cgaagaacag caggctggcg
    29401 tggcccgcga acgagtcggc cgtgaacggt gcgccgccgc tgtcgacgag cgtcggcatg
    29461 gcaaggtcgc gcgcatcggg cagccaggtg cccgattgca gttcgggtgc gggttccgtc
    29521 gtcagggttg cgcgggcgat ccacgcgccg gtgagcgcgg cggcaacgat gagcagcgac
    29581 caggagagcc aggatttcgg ggcggacatg acaggattat cccacattcg gcgcgcgaac
    29641 ccgaccgtac ggcaggtgat cgcgaatgcc acccggcgac tcgcgcgtgc gaagctgcat
    29701 tacggccacg gcaccgacaa tgcgcgcgac gacgcggccg ccatcgtgtg gcacgtgctc
    29761 gggctgcccg tgcccccgcg gtcgcgcgac tatgaccggc gcgtggcggc gcgcgcacgc
    29821 caggacatcg acgcgctgct cgagcggcgc atccgcgaga ggatgccggt cgtatacctc
    29881 acgcagcaga cctggttcgc gggcctgtcc ttccacgtcg atgagcgggt gctgatcccg
    29941 cgctcgccga ttgccgaact gatcgagcgc cgcttcgcgc cgtggatcga tgcaagtcgc
    30001 gttcacgaca tcctcgacct cggcaccggc tcgggctgca tcgcgatcgc ctgcgccaag
    30061 gcattcccgc gcgcgaaggt cgatgccagc gacatttccg ccagtgcgct cgaggtcgcg
    30121 gccatcaacg tgcggcgcca tcgtctgggg cgtcgtgtcc gtctcgtgcg ttccgaccat
    30181 ttcgcggccc tgggcgggcg gacctacgat atcatcgtgt ccaacccgcc ctatgtcggg
    30241 gccgaagagc ttgcggccct gccgcccgag tactggcacg aaccccgcgc ggcgctcgag
    30301 tccggtgacg acggcctcga ctccctgcga gtgatcctgg cggaggccgg gcggcacctg
    30361 cggccccgcg gcatactcgt ggtcgaggtg ggtaattccg aggtcctggc gcgcaggcgg
    30421 tatccgcgcg tgccgttcac ctggctcgag ttcgagcgcg gtggcggcgg agtgttcgtg
    30481 ctgacggcgg aacaggttcg cgatgtccgg taacacattc ggcaagttgt tcacggtgac
    30541 cagctttggt gaaagccacg gcccggcgct cggctgtatc gtcgacggct gcccgccggg
    30601 gctcgagctg tcggccgccg acctgcagcc cgacatcgac cgccgccgca ccggtacgtc
    30661 gcagttcacg agccagcgac gcgaagacga ccaggtacgc attctctcgg gagtcttcga
    30721 ggggcgcacg acgggcacgc cgatcggcct gctgatcgag aacagcgacg cgcgctcgcg
    30781 cgactacgag aagatcaagg accgtttccg ccccggccac gccgactacg cctacctgca
    30841 gaagtacggc gtacgcgact accgcggcgg cggccgttcc tcggcgcgca tcacggtcgc
    30901 gaccgtggcg
//
LOCUS       DAOQVY010000014        30665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_1744_length_30665_cov_6.045247, whole genome shotgun sequence.
ACCESSION   DAOQVY010000014 DAOQVY010000000
VERSION     DAOQVY010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 30665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30665
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1744_length_30665_cov_6.045247"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PKN91_02535"
     CDS             <1..650
                     /locus_tag="PKN91_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNS26978.1"
                     /translation="GEEEHWSLTALFDAMGALSTARNATPELASRAYDVQRDGFVIAS
                     GGGMLVLESYEHARARGAPILAELVGFGVTSDGADMVQPSGEGAVRCMRQALSGVARP
                     VDYVNAHGTSTPLGDVIELNAVREVFGFDLPLLSSTKSLSGHSLGAAGVHEAIYTLLM
                     LQGGFLAGSANIDELDPACADYPILRETRTMRVDTVMSNSFGFGGTNASLVFSRL"
     gene            647..1666
                     /gene="nadA"
                     /locus_tag="PKN91_02540"
     CDS             647..1666
                     /gene="nadA"
                     /locus_tag="PKN91_02540"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNS26979.1"
                     /translation="MNASAPTLERHPYDTELHAQRYARVARLMPVAEWDLHFEQIAAI
                     EALKRERNAVVLAHNYQRPEIFHGIADFQGDSLELARQAGRSTADVIVMCGVRFMAET
                     AKLLAPDRIVLLPDLEAGCSLAESITPADIRALRAQHPGVPVVCYVNTSAAVKSECDA
                     CCTSSNALQVIENLGAPEVIFVPDEYLAAHVAAHTSVRIIAWQGHCEVHQRFTGQEVR
                     EYRETAQAYILAHPECPRDVQEAADYVGSTSGMIAALGRVQPARAVLITECSMADNIS
                     GAFPHIEFLRPCNLCPHMQRITLPAVRASLERLEPAIEIPADVAKGARRAVERMFELG
                     GPRRP"
     gene            1663..3150
                     /locus_tag="PKN91_02545"
     CDS             1663..3150
                     /locus_tag="PKN91_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNS26980.1"
                     /translation="MIRLETDLLVIGAGAAGIVTALSAVGRRVCLLHAAGTASEMAQG
                     GIAAAVGPDDSPSLHAADTFSAGHSLNDARAVQFACEGAPAAVAWLESQGVRFARSDA
                     AWSLHLEAAHSRPRVLHAGGDATGAVIMDALRDAAAAAPHIEWLPRMHALALLCDGAG
                     AGGVTAVSAEGQQVSVHARDVVLATGGIGALYSRTTNPPVARGDGLAMALAAGARCAQ
                     LAAVQFHPTALDVEASPLPLISEAVRGAGAHLVDERGRRIMEAAHPLGDLAPRDVVAA
                     VVHAEQVAGRRVWLDATALRGVEFAQAFPSAWRNCQEHGIDPLREPIPVTCAAHYHMG
                     GIAVDLDGRTSVPQLWAVGEVAYTGLHGANRLASNSLLETVVFGRRLGALLARSGGRV
                     EPGAELAPCAAVDARIGDEIRQLMWRCLGPVRSATTLTTEIARRTALREHASRAASAH
                     ELLVEAMLRAGLAALTSDGTEPQTPVHIPGDPPATPRSHRCPRGS"
     gene            complement(3056..4255)
                     /locus_tag="PKN91_02550"
     CDS             complement(3056..4255)
                     /locus_tag="PKN91_02550"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNS26981.1"
                     /translation="MLTPDEADRIIRAQLRALVVEPRPLLQCAGAVLREEVCAERDQP
                     PFDRVAMDGIALAAQAVREGRRHFRVQGLQAAGDAPHTLADTAHCIEIMTGGMLPRGC
                     DAVVPVERITLREGEATLDDGLEVAPWQNVHRRASDAPAGRRLLAAGTVLNAPEIAVA
                     AAAGRAHLQVTAQPAVIVVSTGSELVEPGEAILPHQVRRSNVYGLVAQLRLHGFTRVA
                     DDHVIDDAVTLRRRLELHLATHDLLVLSGGVSAGRLDLVPQVLAELGVQKHFHKIAQR
                     PGKPLWFGTAPSGAAVFGLPGNPVSSFVCLARYVLPALHVACGCTPRAPEAVTLAAAV
                     RVTAPLTSFLPVRLVRGEGGALFAEPCPTNGSGDFTSLVGTDGFVELPAGPREYAQGF
                     AAPFHHW"
     gene            complement(4249..4692)
                     /locus_tag="PKN91_02555"
     CDS             complement(4249..4692)
                     /locus_tag="PKN91_02555"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNS26982.1"
                     /translation="MGKPVIPLVYSCSGCSSAAQMANQLAIRLDRAGLAEMSCIAGVG
                     GGVAGLVRTARSGRRILALDGCVLACVKACLAQAGVEADRHLVLSDYGVRKRKHADVD
                     QEQASQVYAEHVRPEAQALLATLAAAARETPDADVALQSTQGRAC"
     gene            4962..5696
                     /locus_tag="PKN91_02560"
     CDS             4962..5696
                     /locus_tag="PKN91_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNS26983.1"
                     /translation="MAQRSSRFAGTVTLVAGGTGALGSAVSRAFLDEDATVIVTYRRQ
                     EEFEGLAQAAAEHRSRLEGHRIDATDEAATLRLVDDIVARHRRLDVLVNTVGGYAGGG
                     KLWELDTEVLDRMLALNLRSGYALARAVVPTMLRQAGGSIVNVAAKAALDRPAGAAAY
                     AASKAAALALFDTLAAEVADSGVRVNSVLPRMIDTAANRDAMPQADFSKWPRPEAIAR
                     VILFLCSHDACVIHGAAIPVYGSTTK"
     gene            5693..5962
                     /locus_tag="PKN91_02565"
     CDS             5693..5962
                     /locus_tag="PKN91_02565"
                     /inference="COORDINATES: protein motif:HMM:NF040453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6522 family protein"
                     /protein_id="HNS26984.1"
                     /translation="MSKVEFEDGAISIDATVVAEGFGLSTDQIRHLMRTLKMTTRCER
                     GIDADAGRHRLTFLYGMRHLRLLVDETGAIIERSLIDTGDTGESG"
     gene            6198..8987
                     /gene="polA"
                     /locus_tag="PKN91_02570"
     CDS             6198..8987
                     /gene="polA"
                     /locus_tag="PKN91_02570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNS26985.1"
                     /translation="MDTPADIVLVDGSSYLYRAFHALPPLTSSRGEPTGALLGVLNML
                     LKFLKEQSPRRIAVVFDAPGKNFRDELFAEYKAHRPPMPDELRAQTEPLLEAVRALGL
                     PVLRVPGVEADDVIGTLAKRGEERGWRVLISTGDKDMAQLVGTHITLINTMSNTVLDR
                     AGVKAKFDVYPEQIIDYLALVGDTSDNIPGISKVGPKTAAKWLDRYQTLDGIIENAAA
                     IEGKVGENLRAGLGTLALSRQLATIRTDIELDERPEDLVRGEPDTDALREIYSRFELR
                     SLLRQLDGSGPDDATGGGGQSAAKIPSAPRILATPTAASPDLPTLTPPEVADKRYEIV
                     TTKADLERWLTALAGAELFAFDTETTSLEYMKAEIVGVSFAIEPGHAAYVPLAHDYAG
                     APAQLDRADTLARLAPLLEDPARGKIGHHMKYDAHVLANHGIAFAGMRYDTMLESYVW
                     NSVATRHDMDSAAQRYLGVGTIHYEDVAGKGARQITFNQVDIARAAEYAAEDADITLR
                     LHRTLWPAIAAMPTLERVYTSVEQPLVPVLFAMEDAGVLLDPEMLRAQSRELAQRLAG
                     IEADAHREAGQPFNLDSPKQLQFVLFEKLQIAPLRKTPTGQPSTAEDVLEELADEHAL
                     PRLILEYRSLAKLRSTYTEKLPEQVNPRTGRVHTSYHQAVAQTGRLSSTDPNLQNIPI
                     RTPEGRRIRQAFIAPPGHVLMAADYSQIELRIMAHLSGDEGLLAAFAAGEDVHRATAA
                     EVFGVSLGEVSADQRRSAKAINFGLIYGMSAFGLARQLGIDRGAAQQYVNRYFERYPG
                     VKRYMDTTRERAKQDGYVETVLGRRLYLPDIRSRNRQLQQYAERSAINAPMQGTAADI
                     IKRAMIAVHEWCMRERPPARLIMQVHDELVMEVRADAVAQVGDRLRECMTGAAQLKVS
                     LKVDIGTGANWDEAH"
     gene            9051..9584
                     /locus_tag="PKN91_02575"
     CDS             9051..9584
                     /locus_tag="PKN91_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNS26986.1"
                     /translation="MLRNQLLALGALALFSATATAADNGFYLGAGVTQSEIDGVADDF
                     DMEDTSFKLIAGFRPLDVFAVELNYMDLGSDSISAGPFSADAEARAIAGFGLLMLPLP
                     LVDLYVKAGLAWWESEASTSLSPARLKEDGTEFAYGAGVQVRFWSLAARLEYESFDIE
                     GTDGLDLFTLGVTYTFL"
     gene            complement(9712..10326)
                     /gene="yihA"
                     /locus_tag="PKN91_02580"
     CDS             complement(9712..10326)
                     /gene="yihA"
                     /locus_tag="PKN91_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNS26987.1"
                     /translation="MSRFPNARFMLSAAAAAQFPPDDGAEVAFAGRSNAGKSSAINAI
                     TQRKALARTSKTPGQTRLLNYFELAPGQRIVDLPGYGYAAAPPAERAKWAPLIDQLRA
                     RQSLRGLFLVVDARRGVGEEDERLIAWADPTSRAVHVLLAKADKLTRNEARAALAAAH
                     KQLAGRATVQLLSSHKGDGVPEAQKALLALLAGEREIKGPGSSG"
     gene            10386..11036
                     /locus_tag="PKN91_02585"
     CDS             10386..11036
                     /locus_tag="PKN91_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNS26988.1"
                     /translation="MTKTTLAFAALALAANWTVVQAADGSVEAGAGKAAVCLACHGLN
                     GNSTNPEWPNLAGQNAAYIAEQLKAFRDGKRAGTPNALVMMTQAATLTDEDIADIGAY
                     YAAQTPTGLEADPSYWKDGEALYRGGDHERQIPACMACHGPVGRGNPAAGYPAVRAQH
                     SVYTVQQLNEYADGSRYKDLAGGTQNTRNAHMMVTVAKSLTAEDIRNLASYIQGMR"
     gene            11051..11896
                     /locus_tag="PKN91_02590"
     CDS             11051..11896
                     /locus_tag="PKN91_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HNS26989.1"
                     /translation="MIRVLLFGALLACAASACSRDPGTPPAAEAPATQTAAPAPVPAS
                     EPAAPEEAAEAAQASQESLGDSEDDKSDRPLERLAALPPADELPGGKWKAGVNYKPIV
                     PGQPTSAPPGKVEVNEIFWYGCGHCYALEPFVRSWEKSKADYIEFVRTPVMWNPVNVA
                     HARLFYTLQALKRDDLHAKVFDEIHMRGNMLVANDDTRTRQMQLAFARANGIVEADFL
                     REYDGFYVTSQLQRAEAATRRFQVDAVPVVFVAGKYSTDVGAAGGQKEMLQLIDALAA
                     HEKRR"
     gene            11922..12899
                     /locus_tag="PKN91_02595"
     CDS             11922..12899
                     /locus_tag="PKN91_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNS26990.1"
                     /translation="MLSAFLPGPAALTRLEPHCDAPLPQEALWFDLREPTPEDERRIE
                     TAIGVGIPTREEMREIESSNRLYEEDGALFMTATVVTKLDTDLPESTQITFILAGKRL
                     VTVRYVDPLPFRRFIAYAERHMTNCHSGATIFAGLLEAIINRIADVIERAGAALDAES
                     AGIFAHTAPGRRATSRDFRGILERLGQNGELLSKSRESLVSLGRLLAFAQQQPAPTFT
                     PDVRSRFRMLSRDVLAMSDHVSYLAAKVQFLLEATLGMVNIEQNAIIKIFSVAAVVFL
                     PPTLVASIYGMNFHHMPELDWLAGYPFAIGLMVLSAVLPYLWFKRKGWL"
     gene            12957..13033
                     /locus_tag="PKN91_02600"
     tRNA            12957..13033
                     /locus_tag="PKN91_02600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12991..12993,aa:Arg,seq:ccg)
     gene            13193..14248
                     /locus_tag="PKN91_02605"
     CDS             13193..14248
                     /locus_tag="PKN91_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNS26991.1"
                     /translation="MATYERRKGFWRVKVRRAGFPPQTRSFNSKALAQSWARDVEAKL
                     DRGELVDRSEAHAHTLGDVLKRYLKEVSPHKKGHIPETYKVRSILRSPVCQLRMSVLS
                     SAHIAAYRDERAQSVKPATVNRDLGIISHAIETARREWRIHMPDNPVHRVRRLRAPLM
                     RSRRLSPEEEQRLLAACEESRKPFLLPVVKLALETAMRRGEITRVERKRIGLKHNVLV
                     LSDTKNGSARAVPLSNVAKAIIEKVLVEMPPTPDGLLFAGVTNEAIKLAFQRAVKRAK
                     LDDFRFHDLRHEATSRLHEKGLNPIEVMSVTGHKSMSSMLRYSHVRGLAVTEKLNRPL
                     DYQPLVHQLTENRHEGR"
     gene            14235..14627
                     /locus_tag="PKN91_02610"
     CDS             14235..14627
                     /locus_tag="PKN91_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26992.1"
                     /translation="MKVGDRARCSTRRRRRLPVPLARVPTVYPVATPRRAKPKGGAFR
                     AFLRRLRDRMHRFVFVPGSCAWMWRDGQTNPAMGLGMYGGVDSAGNPYGVGSIHVASH
                     RDWQAAYASGISSLPCVVIEHDSAWRDW"
     gene            14685..15692
                     /locus_tag="PKN91_02615"
     CDS             14685..15692
                     /locus_tag="PKN91_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26993.1"
                     /translation="MLEKAPPNPLTDAAIQRYLHDTLGLPTSLRPWSGAGKLPYFLQE
                     AFATRELTLHGQTIVLAEEQRRHKPKLAELRTQMDRLRAAAQLPVIYVTDALASYERK
                     RLVEQRVPFLVPGNQLYLPDLGIDLREYFRQPTPAIETGLSPATQAVLITMLLTKPWR
                     QDWTLGETVAQLGYTPMTLTRVLRELTATGLAKTRRLGREHHLYTDQPPEKLWDSLQA
                     QLRKPVKRSIWVKTAKQENTGLRLAGMSALARLTQIADPPWPVYAISPTDWRAAKPLV
                     QLLKEPAAGCSEWQLWTYSPALVPNARTVDPLSLTLSLDPGADERMQMALDELRKQFP
                     W"
     gene            15686..16441
                     /locus_tag="PKN91_02620"
     CDS             15686..16441
                     /locus_tag="PKN91_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26994.1"
                     /translation="MVRGLDLFRQFFAAHADQYVLIGGTAATLAMEAAGLPFRATKDL
                     DVVLHIEALDAKFGESIWNFIEAGRYEIRQASATGKPIFYRFQRPADENYPAMLELFS
                     RAPEGIALAPGSHLTPIPIDEAVASLSAILLDNDYYEFVLSGRRDLDGLPWVGEDRLI
                     PLKAVAWLDMKARASRGETVDARDIRKHANDVLRLSQLLAPDTRINATERIGADINRF
                     LDGVLAEGDINPADLGLRGSLAQILARVRNAYT"
     gene            16438..18834
                     /locus_tag="PKN91_02625"
     CDS             16438..18834
                     /locus_tag="PKN91_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNS26995.1"
                     /translation="MSKSRGNEPGSLAALQAENIRLIALLESRGIPWQLPQEPTPVAE
                     VTEPSRLTADEKVALFRRLFRGRPDVYPVRWESKTTGKSGYAPACANEWRPGVCDKPR
                     IRCADCNFRSLTPVSDSIIYSHLAGKHTIGVYPLLEDDTCHFLAVDFDEATWREDAKA
                     FRVSCAELGVPVALEISRSGNGAHAWVFFSGRVPARDARRLGTAIISHTCARTRQLAL
                     TSYDRLFPNQDTMPKGGFGNLIALPLQKYPREQGCSVFVDDTLQPYTDQWGYLATVQP
                     MAPHDIDSTILRATGNNHPLDVRFIEDEDLAAPWKSRPAPSRRLPDKMPESLTVTLAN
                     QIYFEKAKLPQALANRLIRLAAFQNPEFYRAQAMRMSVWDKPRVIGCAENFPQHIALP
                     RGCLDVVSELLAENSIRVALIDERYSDKPIGAEFTGTLRADQEAAAAAMLPHDAGVLC
                     APTAFGKTVTAASLIARRGVNTLILVHRTELMRQWQERLSAFLNAAGTPVGVIGGGKN
                     RPTGVIDIAVMQSLSRSGEVDPLVEGYGHIIVDECHHVGAVSFDAILRRARAKFVLGL
                     TATPIRRDGQQPIIFMQCGPVRHTASRPAGAPADLEVVPRSIYAPLDVPADAGIQDVF
                     RHLAGDQTRTAAIAAEIRSAADQGRKILVLTERADHIDALRAACGDREPAPFVLHGRM
                     SRKLRSTLIAQLNALPPDAPRILLATGRLVGEGFDHPPLDTLVLAMPVSWKGTLQQYA
                     GRLHREHVGKANVRIIDFVDAGHPVLRRMWDKRQRGYKAMGYRIATEGLFDRQHQFKE
                     "
     gene            complement(18824..19480)
                     /locus_tag="PKN91_02630"
     CDS             complement(18824..19480)
                     /locus_tag="PKN91_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26996.1"
                     /translation="MNEEAARARRIADEAHHKHDVALQQLEKLGRETHTTNLDHAKLL
                     ADIRHRWPKYIKAEAEEIALAQARACSKATVATVETRLHVVEGGLDQLMGRVQAVVNR
                     AESISSSLQWKTLGHAGLIALGTIALLFPLSMWWGSRSMAATAGELMRVQAAGYERLR
                     ELSKAEFRQCEVNGSQRLCVRIEPDVPTTEGKAGAVYAVVHGYGTKSIAPKETAEFTP
                     "
     gene            complement(19473..20882)
                     /locus_tag="PKN91_02635"
     CDS             complement(19473..20882)
                     /locus_tag="PKN91_02635"
                     /inference="COORDINATES: protein motif:HMM:NF019291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HNS26997.1"
                     /translation="MNTQVSFALRGRNPDVLTCIANLSNDEVFTPPEFANQMLNALAA
                     AWSAGHNGANIWADRNVRFLDPCTKSGVFLREIAKRLTIGLTNEIPDLNERVNHILTK
                     QVFGIGITHLTGMLARRSVYCSKHAQGDHSIAKGFASDAGNVWFERTEHVWMEGRCTY
                     CGASKKALDRGDALETHAYAFIHTNDIKGWIAGLFGGDMQFDVIVGNPPYQMTGGGGG
                     TNDSPIYQMFVRQAMQLEPRFLSMVIPSRWMAGGRGLGDFRSEFLGDRRLRMLVDYEN
                     AKDAFPTVGIGGGICYFLWDRDNPGRCESIYHRNGATIGPHARDLDEFDVFVRDKRAV
                     DILRKVLTREEGSFEDLVSGDTPFGLATNFADYVQDKPPKAGQVRLYANVGTRRVRGA
                     TARKGISKNTQMIDVWKLFLPVAGSGRERERSGVDLVLGPSIVGEPGSVCTQTYLVAG
                     AFSSKEEAQLAQRVYPCIE"
     gene            complement(20879..21535)
                     /locus_tag="PKN91_02640"
     CDS             complement(20879..21535)
                     /locus_tag="PKN91_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNS26998.1"
                     /translation="MSLVKSKQRVADHGEVFTPAWLVEAMLDLVKEESERIDSRFLEP
                     ACGSGNFLVQVLRRKLAAVELKYGKSDFERRHYALLGLMCIYGIELLADNIAECRANL
                     LEILAEYLNLSASDDLYRAASFVLSQNLVHGDALTMCNHEGKPITFAEWGYLGKGRFQ
                     RRDFRFDVLLGSSAFSAEGSLFAHLGKHEIFTPVKTYPLVTSRELAAEATGVERKRAV
                     "
     gene            complement(21532..21966)
                     /locus_tag="PKN91_02645"
     CDS             complement(21532..21966)
                     /locus_tag="PKN91_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNS26999.1"
                     /translation="MAEARRVVLDTNTVLSALLFVNGRLVPLRAAWQSGDLTPLLCAQ
                     TVEELLRVLAYPKFKLTPEERDELLADYLPYGEVVAPWQAKPAVPECRDECDQIFLDL
                     AAVGGAQWLVTGDQDLLSLNGQVKFQIIAPVAALELLTSAES"
     gene            complement(21966..22199)
                     /locus_tag="PKN91_02650"
     CDS             complement(21966..22199)
                     /locus_tag="PKN91_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNS27000.1"
                     /translation="MLAKMTAKNQLTLPKAITKAVGDPRYYEVRTEDGQIVLTPVRIQ
                     RADAVRAKLAELQISEQDVEDAVTWARSAGSQR"
     gene            22337..22762
                     /locus_tag="PKN91_02655"
     CDS             22337..22762
                     /locus_tag="PKN91_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNS27001.1"
                     /translation="MRVVLDTSVLVAGLRSQLGASNLILMAAAEGTFRPLASTAVFLE
                     YEAVLMRAEQRLATGMSAEDVQGFLAAFASAADAVEIDFSWRPQLRDPSDELVLEAAV
                     NGQATFIVTHNVADFEGGAARFGIQTLTPGQFLKELRKP"
     gene            22759..23007
                     /locus_tag="PKN91_02660"
     CDS             22759..23007
                     /locus_tag="PKN91_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27002.1"
                     /translation="MSKSTYPLKLPNSIKDAAAELARLDGVSLNQFIAAAVAEKVGVL
                     RTASEFLKRRAGSATPQDMVRYLRAAPKLPPMERDEIG"
     gene            complement(23219..23371)
                     /locus_tag="PKN91_02665"
     CDS             complement(23219..23371)
                     /locus_tag="PKN91_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27003.1"
                     /translation="MRLRQRRACRLVDALHERAAAFYESFGFLPLPESLRLGLPMRSI
                     EALLAR"
     gene            complement(23538..24233)
                     /locus_tag="PKN91_02670"
     CDS             complement(23538..24233)
                     /locus_tag="PKN91_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNS27004.1"
                     /translation="MSFVLPPPPQPSVGIKDSDARFPVHRIYCVGRNYAAHAREMGMD
                     PEREPPFFFTKPADAITPNHATIPLPSRTANLHHEVEPVVALGKGGRDIAPAQSLGHV
                     YGYAVGNDLTRRDLQFEARDKGRPWDTGKGFDRSAAISAITPATQSGHLGSGRIWLAV
                     NGEMRQNADLSELIWSVPEIIAELSTLFELAPGDLIYTGTPAGVGPLQPGDRIEGGID
                     GLETLVTTIAPRR"
     gene            complement(24474..25334)
                     /locus_tag="PKN91_02675"
     CDS             complement(24474..25334)
                     /locus_tag="PKN91_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNS27005.1"
                     /translation="MSAEQIRPIDRLSALLARFRVHARLFHAGALCGRTNLPAEPGRG
                     FLHVLREGELVVKHRAGSGAPRRIELTEPTLLFYPRPVTHEFVHAPGEGPNLVCAALD
                     FDGGASHPLVRALPPLLALPLKSVQGMEHTLELLFAEAERVRCGQRLLADRLFEVLLV
                     QLLRWLLDHPAEAGLPAGLLTGLSHPKLARTLTALHEQPGAPWSLQSMADTAGMSRSA
                     FATAFREWVGQTPAEYLLKWRVSIAQTLLRDGAPIKSVGDQLGYASASSLSRAFTQAV
                     GVSPRAWLRQ"
     gene            25438..25983
                     /locus_tag="PKN91_02680"
     CDS             25438..25983
                     /locus_tag="PKN91_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNS27006.1"
                     /translation="MNRIPLIDRNATSPDRKALLDTINGAFGMTPNMFRAVANSPAAL
                     KSMWGSFGALGAGVIPAKLGEQIAVAVANRNGCEYCLAAHTALGRSAGASATELRDAQ
                     AGVSADARTQAALHFALKLVDKRGQIEAADVQALRTAGFGDAEIVEILAHVALSLFTN
                     YVNVALAVPVDFPGVKLREAA"
     gene            26008..26493
                     /locus_tag="PKN91_02685"
     CDS             26008..26493
                     /locus_tag="PKN91_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HNS27007.1"
                     /translation="MTDVAAPALQVSDWLNAPRDFSLAALRGQVVLLHAFQMLCPGCV
                     AHGIPQAQRVHRLFGTEALAVIGLHTVFEHHEVMNAAALRVFVHEYHLAFPVGIDQPG
                     DGDPVPLTMRAYGFRGTPSLALIDRQGRLRLQHFGQIDDLQLGAAIGRLLAEPAPPAA
                     P"
     gene            complement(26548..27267)
                     /locus_tag="PKN91_02690"
     CDS             complement(26548..27267)
                     /locus_tag="PKN91_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNS27008.1"
                     /translation="MGARAFAPPEGSPWQPGNLELFRSLSEPALDAVRAAMRVDRVAK
                     DDVIFVQGDRAERAYALGAGSVRIMQIGSDGGQAIVRFIAPGETFGTVPLFTDHRLPA
                     DAIAAEDSVVLSWSEAELRRLIALFPPIAMNVIEVLGARLGQLQERLRELTTQRVEQR
                     IAHAVLRLAAQAGRDGSGGTTIAFPLRRKDIAEFSGTTLHTASRTLAAWEKAGLLASR
                     NGYLTIRDPDGLRRVAEDCAP"
     gene            27389..29614
                     /locus_tag="PKN91_02695"
     CDS             27389..29614
                     /locus_tag="PKN91_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS27009.1"
                     /translation="MPANYPRKPRAGLALAALLLSAGLAPSGVSAQSATPGAVLDEVV
                     VSASRDRPVVRLDAAAGGTAIVTSREMPASTHLTLSRAVADVPGVVVQDFFGGNDQPR
                     VQIRGSGLQQNPVERGILMLRNGLPLNRADGSYVVGFADPGAADAIEIFRGYMANRLG
                     ATVLGGAFNLISPTGRSAPGFALAAGGGSFDLLGGNAQYGYAHEHIDLLLQGGYTERE
                     GFRDYNASRRGNFGGNLGLRLSDTLTVRLFAGHVDLSFDVAGPLTRELLERDPRAVFT
                     GPSVVPGGAINPGPNVVRDRPRRDASQFQAGARATNTYGAHVVDFAIGRARTDDSFRF
                     PMAAGIRVTEGDDTTAVLRYAFKPDATRVLPLFEATAQYVTGAADRDNHLNLSGRRGA
                     LFGSSRLEADTLSLNAGLNIALTGRLSLSPSVSWSHASRDNDDRYDLPTRPTAAYSPA
                     DPAVALPNGAVPTVDSSYARRYKAWSPALGLTWQPSGDQTLYLAVSRSFEPPTHDDLL
                     ATVNGTPNSSAGRPNPAAPAQPAAAFVTPALEAQRATTVEAGWRGDSGPMSWDAAVYH
                     SWVRDELLSLRDESGVSLGAANAPRTRHLGVELGLTARLASTFTGRIVYTYQDFRFRD
                     DPVRGDNRLAGAPRHWIHGKLSWQPAANWNAEAAARWMPGETPVDNLGTLYNDAFATV
                     DLRVERVFGRALSVFAEVTNLLDETHAASALIVDQARSDQAAFLPGDGRAVGAGMRLR
                     F"
     gene            29648..30091
                     /locus_tag="PKN91_02700"
     CDS             29648..30091
                     /locus_tag="PKN91_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27010.1"
                     /translation="MRHRTVLLAALASLIVPGMAAADSTLLELERLNYETVYVAARRT
                     VLALDSEGRPLTALLRIAKGQFLPPHGAEGGLRVLTVLSGTLSWGDGAKVDLAAERTF
                     GPGSVIVVPAAGGGHWAAARQDDVLLQIVMVRQGKLAPEAAAQLE"
     gene            30091..30564
                     /locus_tag="PKN91_02705"
     CDS             30091..30564
                     /locus_tag="PKN91_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27011.1"
                     /translation="MMELALARALHVLGVVVWIGGVSMATSVALPAIRRGVFGADRLQ
                     AFHAFEGRFIWQARTAVVIVGLTGFYMTERLGLWWRFGDGHYWWMHAMLIVWSLFMLL
                     LFIGEPFVLHRFFPVWVKRDPGRAFAFLHTVHVVLLVLSLVTVFGAVAGSHGWMP"
BASE COUNT         5340 a        10447 c         9794 g         5084 t
ORIGIN      
        1 gcggcgagga ggagcactgg tcgctcacgg cactgttcga cgcgatgggc gcgctgtcca
       61 cggcgcgcaa cgccacaccg gaactcgcct cgcgcgccta cgacgtgcag cgtgacggct
      121 tcgtgatcgc aagcggcggc gggatgctgg tgctcgaatc gtacgagcac gcccgcgcgc
      181 gcggcgcacc gatccttgcc gaactcgtcg gcttcggcgt cacctcggac ggcgcggaca
      241 tggtgcagcc ttcgggcgaa ggcgcggtgc gctgcatgcg ccaggcgctc agcggggtcg
      301 cgcgcccggt ggactacgtg aacgcacacg gcacctcgac accgctcggc gacgtgatcg
      361 agttgaatgc ggtccgcgaa gtcttcggct tcgacctgcc gctgctgtcc tcgaccaagt
      421 cactgtcggg ccattcgctc ggcgccgccg gcgtgcacga ggcgatctac acgctgctga
      481 tgctgcaggg cggcttcctt gccggctcgg ccaacatcga cgagctcgat ccggcctgcg
      541 ccgattatcc gatcctgagg gagacgcgaa cgatgcgcgt cgataccgtg atgtcgaaca
      601 gcttcggctt cggcggcacg aacgcatcgc tcgtgttcag ccggctgtga acgccagcgc
      661 gccgacgctc gagcgtcacc cctacgatac cgagctgcac gcgcagcgct atgcacgcgt
      721 cgcacggctg atgccggtcg ccgaatggga cctgcacttc gagcagatcg ccgccatcga
      781 agcgctgaag cgcgagcgca atgcggtggt cctcgcgcac aactaccagc gtccggagat
      841 cttccacggc atcgccgact tccagggcga ctcgctcgaa ctcgcgaggc aggccgggcg
      901 ctcgaccgcc gacgtcatag tgatgtgcgg cgtgcgtttc atggcggaga cagccaagct
      961 gctcgcaccg gaccggatcg tgctgctgcc tgacctcgaa gccggctgct cgctcgccga
     1021 atcgatcacg cccgcggaca tccgcgcatt gcgcgcgcag catcccggcg tgcccgtcgt
     1081 gtgctacgtg aacacgagcg ccgccgtgaa gagcgagtgc gacgcatgct gcacgtcgtc
     1141 caatgcactg caagtcatcg agaacctcgg cgcgccggag gtcatcttcg tgccggacga
     1201 gtatctcgcg gcgcacgtgg ccgcccatac gtcggtgcgc atcattgcgt ggcagggcca
     1261 ttgcgaagtg caccagcgct tcaccggaca ggaagtgcgt gagtatcgcg agacggcgca
     1321 agcctacata ctcgcgcacc ctgaatgccc gcgcgacgtg caggaggcgg ccgactacgt
     1381 cggctcgacc tcgggcatga tcgccgcact cgggcgcgtg cagcccgcgc gcgcagtact
     1441 gatcaccgaa tgctcgatgg ctgacaacat ctcgggagcc tttccgcaca tcgagttcct
     1501 gcgaccctgc aatctctgtc cgcacatgca acgcatcacg ttgcccgcag tgcgcgcgag
     1561 ccttgagcgg ctcgaacccg cgatcgagat cccggcggat gtcgcgaagg gcgcacgccg
     1621 ggcggtggaa cggatgttcg agctcggcgg cccgcgccgc ccgtgatccg gctcgaaacg
     1681 gatctgctcg tgatcggcgc cggggcggcc ggcatcgtca cggcattgag tgccgtggga
     1741 cgccgcgtgt gcctgctgca tgcggcaggc acggcgagcg agatggccca gggcggcatc
     1801 gccgctgcgg tcggcccgga cgattcacca agcctgcacg ccgcggacac gttcagcgcg
     1861 gggcactcac tcaacgacgc gcgcgcggtg cagttcgcct gcgagggcgc acctgcggcc
     1921 gtggcctggc tcgaatcgca gggtgtgcgc tttgcgcgca gcgacgcggc gtggtcgctg
     1981 cacctcgagg ccgcgcacag caggccgcgc gtgctgcatg ccggcggcga cgccacgggc
     2041 gcggtcatca tggacgcgct gcgcgatgca gccgcagccg cgccgcacat cgaatggctg
     2101 ccgcgcatgc acgcgctcgc cctgctctgc gacggggctg gcgcgggcgg ggtgacggca
     2161 gtatccgccg agggccagca ggtctcggtg catgcccgtg acgtggtcct tgcgaccggt
     2221 ggcatcggcg cgttgtacag ccgcacgacc aacccccctg tggcgcgagg cgatggcctg
     2281 gcgatggcgc tcgccgccgg cgcgcgctgc gcgcagctcg cggcggttca gttccatccc
     2341 acggcactcg atgtcgaggc gagtcccctg ccactcatct ccgaagccgt gcgcggcgcc
     2401 ggcgcgcacc tggtcgacga gcgcggacgg cgcatcatgg aagccgcgca tccgctcggc
     2461 gatctcgcgc ctcgcgacgt cgtggccgcc gtcgtgcatg cggagcaggt ggcgggccgg
     2521 cgcgtctggc tcgatgcgac ggcgctgcgc ggggtggaat tcgcccaggc gtttccgtcg
     2581 gcctggcgaa actgccagga acacggcatc gatccgcttc gcgagccgat ccccgtgacc
     2641 tgtgccgcgc actaccacat gggcggcatc gcagtggatc tcgacggccg cacgagcgtg
     2701 ccgcagctgt gggcagtcgg tgaggtcgcg tacaccggcc tgcacggcgc gaaccggctc
     2761 gcgagcaact cgctgctgga aaccgtcgtc ttcggccggc ggctgggagc cctgctcgcg
     2821 cgctcgggcg gccgtgtaga gccgggcgcc gaacttgcgc cctgcgcggc ggtggacgcg
     2881 cgcatcggcg acgaaatccg gcagttgatg tggcgctgcc tcggccccgt acgcagcgcg
     2941 acaacgctca cgacggaaat cgcgcggcgc acggccctgc gtgaacacgc gtcccgggcc
     3001 gcctccgcac acgaactgct ggtcgaggcc atgctgcggg cgggcctggc cgccctcacc
     3061 agtgatggaa cggagccgca aacccctgtg catattcccg gggacccgcc ggcaactcca
     3121 cgaagccatc ggtgcccacg aggctcgtga agtcaccgga gccgttcgtc ggacagggtt
     3181 cggcgaacag cgccccgccc tcgccgcgca cgagccgcac tggcaggaag gatgtcagcg
     3241 gcgccgttac acgcaccgcg gccgcgagag tgacagcctc gggcgctcgc ggcgtgcatc
     3301 cgcaagcgac gtgcagcgcc ggcaggacat agcgcgcgag gcacacgaac gacgagaccg
     3361 gattgcccgg caacccgaac accgcggcgc ccgatggcgc ggtaccgaac cacagcggct
     3421 tgccggggcg ctgcgcgatc ttgtggaagt gcttctgcac gccgagttcg gcgagcacct
     3481 gcggcacgag gtcgagccgc ccggccgaca cgccaccact cagcaccagc agatcatgcg
     3541 tggcgaggtg caactcgagc cggcggcgca gcgtcaccgc gtcgtcgatc acgtggtcgt
     3601 ccgcgacgcg ggtgaacccg tgcaggcgca gctgcgcgac gaggccgtag acattggagc
     3661 gtcgcacctg gtgcggcagg atcgcctcgc cgggttccac gagttcgctg cccgtcgaca
     3721 cgacgatgac cgccggctgc gccgtaacct gcaggtgggc ccggcccgcg gcggccgcca
     3781 cggcgatctc cggcgcgttc agtaccgtgc ccgccgcgag caggcgcctg cctgcaggcg
     3841 catcgctcgc gcgacgatgg acattctgcc agggtgcgac ctcgagaccg tcgtcgagcg
     3901 tcgcctcgcc ctcgcgcagc gtgatccgct cgaccggcac gacggcatcg caacctcgcg
     3961 gcagcatccc gccggtcatg atttcgatgc agtgcgctgt atcggcgagg gtgtgcggcg
     4021 cgtcgccggc ggcctgcaga ccttgcacgc ggaaatgcct gcgaccctcc cgcaccgcct
     4081 gcgcggcgag cgcgatgccg tccatggcga cgcgatcgaa agggggttga tcgcgctcgg
     4141 cgcagacctc ctcgcgcagc acggcgcctg cacactgcag cagcggccgt ggttccacga
     4201 cgagcgcgcg caactgcgcc cggatgatcc ggtcggcctc gtcaggcgtc agcatgcgcg
     4261 accctgggtg gattgcaggg cgacatcggc atccggcgtc tcgcgcgcgg ctgcggccag
     4321 tgtcgcgagc agcgcctgcg cctccggccg tacgtgctca gcgtaaacct gcgaggcctg
     4381 ctcctggtcc acgtcggcgt gtttgcgctt gcgaacgcca tagtcggaga ggacgagatg
     4441 ccgatcagcc tcgacgccgg cctgcgcaag gcaggccttc acgcaggcga ggacgcagcc
     4501 gtcgagcgcg aggatgcggc gcccgctgcg tgcggtgcgc acgagtccgg ccacgccgcc
     4561 accgacgccg gcgatgcacg acatttcggc aagacccgcg cgatcgaggc ggatcgcgag
     4621 ctggttcgcc atctgcgctg cgctcgagca gccggagcag gagtagacga gcggtattac
     4681 aggctttccc acggaagatt tcctcgcggc cagtataagc gcgcgttcga gggtggcata
     4741 tctgattgtt gcagtcgcgc gcgcgcaaag acttgatccg cagcaacaca attggggcat
     4801 cgtgatccac taggtgccgg tcgccagcag aaagtagaag tcgagttcga gacggtgggg
     4861 atggccgcgc gagtggcggc aatgcgaccc gtgcacgccg cggctggatt accgggccaa
     4921 tgtgctgcac gatcaccctc ctcagccgac aggagcattc agtggcgcaa cgcagtagcc
     4981 gttttgcggg aacagtgact ctcgtggccg gtggcacggg cgcgctcggc agcgctgtga
     5041 gccgtgcgtt cctcgacgaa gacgccaccg tgatcgtcac ctaccgcagg caggaggagt
     5101 tcgagggcct cgcgcaggcc gcagcggagc accgctcgcg actggagggc catcgtatcg
     5161 acgccaccga cgaagcggcc acgctccggc tggtcgacga catcgtcgcg cgacaccgac
     5221 gccttgacgt gctggtcaac acggtcggcg gctacgcggg cggcggcaag ctgtgggagc
     5281 tcgacacgga ggtgctcgat cggatgctcg cgctgaatct gcgctcgggc tatgcgctcg
     5341 cccgcgcggt ggtgcccacg atgctgaggc aagcgggcgg atcgatcgtc aatgtcgccg
     5401 ccaaggcggc gctcgatcgg cctgccggtg cggccgccta cgcggcctcc aaggccgcgg
     5461 cgctggcgct gttcgatacg ctggccgccg aggtggctga cagcggcgtg cgcgtcaatt
     5521 cggtgctgcc ccgcatgatc gacaccgcgg ccaaccgcga cgcgatgccg caggccgatt
     5581 tctcgaagtg gccacgcccc gaggccatcg cgcgcgtgat cctgttcctg tgcagccacg
     5641 acgcctgcgt gattcacggc gctgcgattc ccgtctacgg cagtaccacg aaatgagcaa
     5701 ggtggagttc gaggatggcg ccatcagcat cgatgccacg gtcgttgccg agggcttcgg
     5761 gttatcgacc gatcagattc gacacctgat gcgtacgctg aagatgacca cccgttgcga
     5821 gcgcgggatc gacgcggacg ccgggcgcca tcgcctgacc ttcctctacg gcatgcggca
     5881 cctgcgcctg ctggtcgatg aaaccggcgc catcatcgag cgctccctca tcgataccgg
     5941 cgataccggg gagtccggct gacgagctgc tagtcgcgcc gcaactcgaa gagtcccccg
     6001 cgacgatcga cggcgaggga gttcgcgatc cggccgaccc ggatcgagat gcccgggcgc
     6061 gaaatcgcgg cgcgtgattc ttcagttgca tgccgctgat tgaacctggc ctgcgtgcgg
     6121 acatgcggcg cacgccgggg tcgcgtggcc ccggccacgt gcgctcctcg cccggttctg
     6181 caagactgtc gcttccgatg gataccccag ccgacatcgt cctcgtcgac ggctcctcct
     6241 atctctaccg cgcgttccac gcgctgccgc cgctcaccag ttcccgcggc gagccgaccg
     6301 gcgcgctgct cggcgtgctg aacatgctgc tgaagttcct gaaggagcag tcccccaggc
     6361 gcatcgccgt ggtgtttgac gccccgggaa agaacttccg cgacgagctg ttcgccgagt
     6421 acaaggccca tcgcccgccg atgccggacg agctgcgcgc gcagaccgag ccgctgctcg
     6481 aggcggtgcg cgcgctcggc ctgccggtgc tgcgcgtgcc cggagtcgag gccgacgacg
     6541 tgatcggcac actcgcgaag cgcggcgagg agcgcggctg gcgcgtgctg atctcgaccg
     6601 gcgacaagga catggcgcag ctcgtcggca cccacatcac gctgatcaac acgatgtcga
     6661 acacggtgct cgatcgcgcc ggcgtgaagg cgaagttcga cgtctatccc gagcagatca
     6721 tcgactacct cgcgctggtc ggcgacacct cggacaacat cccgggcatc agcaaggtcg
     6781 ggccgaagac cgccgcgaag tggctcgatc gctaccagac actcgacggc atcatcgaga
     6841 acgcagccgc gatcgaaggc aaggtcggcg agaacctgcg cgccggcctg ggcacgctgg
     6901 cgttgtcgcg ccagctggcg acgatacgca ccgacatcga actcgacgag cgccccgagg
     6961 atctggtgcg cggcgagccc gataccgatg ccctgcgcga gatctactcg cgcttcgaat
     7021 tgcgcagcct gctgcgacaa ctcgatggca gcggcccgga cgacgccacc gggggtggcg
     7081 gacagtccgc ggcgaagatc ccgtcggcgc cgcgcatcct cgcgacgccg accgccgcca
     7141 gcccggacct ccccacgctt acgccgcccg aggtggccga caagcgctac gaaatcgtga
     7201 ccaccaaggc cgatctcgag cgctggctca cggcgctcgc gggcgcggag ctgttcgcgt
     7261 tcgataccga aacgacgagc ctcgagtaca tgaaggccga gatcgtgggc gtgtcgttcg
     7321 cgatcgagcc cgggcacgcg gcctacgtgc cgctcgcgca cgactacgcc ggcgcgcccg
     7381 cccagctcga ccgagcggac acgctcgcac gccttgcgcc gctgctcgag gatccggcgc
     7441 gcggcaagat cgggcaccac atgaagtacg acgcgcacgt gctcgccaat cacggcatcg
     7501 cgttcgccgg catgcgttac gacacgatgc tcgagtccta tgtctggaac agcgtcgcga
     7561 cgcgccacga catggactct gccgcgcagc gctatctcgg cgtcggaacg atccattacg
     7621 aggatgtcgc cggcaagggc gcccggcaga tcacgttcaa ccaggtggac atcgcgcgcg
     7681 ccgcggaata cgccgccgaa gacgccgaca tcacgctgcg cctgcaccgg accctgtggc
     7741 ccgcgatcgc cgcgatgccg accctcgagc gggtgtacac atcggtcgaa cagccgctcg
     7801 tgccggtact gttcgcgatg gaggacgccg gcgtgctgct cgatccggag atgctgcgcg
     7861 cgcagagccg cgaactcgcg caacgcctgg ccgggatcga agccgacgca caccgcgaag
     7921 ccggccagcc gttcaatctc gattcgccga aacagctgca gttcgtgctg ttcgagaagc
     7981 tgcagatcgc gccgctgcgc aagacgccga ccggacaacc ttcgaccgcg gaagacgtgc
     8041 tcgaggagct cgccgacgag cacgcgctgc cgcgcctgat cctcgaatac cggagcctcg
     8101 cgaagctgcg ctcgacctac accgagaagc tgcccgagca ggtcaatccg cgcaccgggc
     8161 gcgtgcacac ctcgtatcac caggccgttg cgcagaccgg gcgcctctcc tcgaccgatc
     8221 cgaacctgca gaacatcccg atccgcacgc ccgagggccg ccgcatccgc caggcgttca
     8281 tcgccccgcc gggccacgta ctgatggccg ccgactattc gcagatcgag ctgcgcatca
     8341 tggcgcacct gtcgggtgat gaaggcctgc tcgcggcgtt tgccgcgggc gaggacgtgc
     8401 accgcgcgac cgcggccgaa gtgttcggcg tgtcgctcgg ggaagtgagc gccgaccagc
     8461 gccgctccgc gaaggcgatc aatttcgggc tgatctacgg catgtcggcc ttcggccttg
     8521 cgcgccagct cggcatcgac cgcggcgccg cgcagcagta tgtcaaccgt tacttcgagc
     8581 gctaccccgg cgtgaagcgc tacatggaca cgacgcgcga gcgcgcgaag caggacggct
     8641 acgtcgagac cgtgctgggc cggcgcctgt acctgcccga tatccgctcg cgcaaccgcc
     8701 agctccagca atatgcggag cgcagcgcga tcaacgcgcc gatgcagggc acggctgccg
     8761 acatcatcaa gcgcgcgatg atcgcagtgc atgaatggtg catgcgggag cgcccgcctg
     8821 cgcgcctcat catgcaggtg cacgacgaac tcgtgatgga agtgcgcgcc gatgcggtgg
     8881 cgcaggttgg cgacaggctg cgtgaatgca tgactggcgc ggcgcaattg aaggtgtcgc
     8941 tgaaagtaga catcggcacc ggcgcgaatt gggacgaagc gcactgacac gggatctgta
     9001 ccagtagaat gtgcgggctt gcttccgtct ccatccacga ggaacgaacc atgcttcgca
     9061 atcagctcct ggccttgggt gcgctcgcgc tcttctccgc gaccgctaca gccgccgaca
     9121 acggcttcta tctcggagcc ggcgtcacgc agtccgagat cgacggcgtg gcggacgatt
     9181 tcgacatgga ggacaccagc ttcaagttga tcgccggatt ccgtcccctc gacgtgttcg
     9241 cggtcgagct gaactacatg gacctcggca gcgacagtat ttcggcaggt ccgttctcgg
     9301 ccgacgccga ggcgcgcgcg atcgcgggat tcggcctgct catgctgccg ctgccgctgg
     9361 tcgacctgta cgtgaaggcc ggcctcgcct ggtgggaatc cgaagccagt acctccctgt
     9421 cgccggcaag gctcaaggag gacggcaccg agttcgccta cggcgccggg gtccaggtgc
     9481 gcttctggag cctcgccgcg cggctcgagt acgaatcctt cgatatcgag ggtaccgacg
     9541 ggctcgacct cttcaccctg ggcgtcacct acactttcct gtagggccac ggaaccattt
     9601 cgcgcgggtc ccgtctaatt tgatgagcgc cataagcgct ccccgcttcc ctccctgagc
     9661 gggtatggcc cggatgcaag agacctgtgc cgggtatgcg gccccggtgg ttcaccctga
     9721 actgccgggg ccttttattt ccctttcgcc cgcaagaagc gcgagcagcg ccttctgggc
     9781 ctccggcacg ccatcgccct tgtgcgacga cagcagctgg acggtcgcgc ggccggccag
     9841 ctgcttgtgc gccgcggcca gcgcggcacg cgcttcgttg cgggtcagct tgtcggcctt
     9901 ggcgagcagg acgtggactg cccgggacgt cgggtcggcc caggcgatca ggcgctcgtc
     9961 ttcctcgccc accccgcggc gggcgtccac cacgaggaac aggccacgca gggactgccg
    10021 ggcccggagc tggtcgatca ggggcgccca cttcgcccgc tcggccggtg gtgcggcagc
    10081 gtacccgtaa ccgggcaggt ccacgatccg ctggccgggc gccagctcga aataattgag
    10141 cagccgggtc tggcctggcg tcttgctggt gcgcgcgagc gccttgcgct gggtgatggc
    10201 gttgatggcg ctcgacttgc cggcattgga acggccggcg aacgcgactt cggcgccgtc
    10261 gtcgggcggg aactgcgcgg cggcggccgc gctcagcatg aagcgggcgt tggggaagcg
    10321 gctcacgatt ggtccttcgg gaagtgtaca attctaaagt tacgcgaccc ggagctgtga
    10381 gaagaatgac gaagacaacc ctggccttcg ccgccctggc ccttgcggcc aactggaccg
    10441 tagtacaggc cgccgacggt tcggtggaag ccggtgccgg caaagccgcc gtgtgcctcg
    10501 cctgccacgg cctgaacggc aacagcacga atcccgaatg gcccaacctc gccggccaga
    10561 acgccgcgta tatcgccgaa cagctgaagg cgttccgcga cggcaagcgc gccggcacgc
    10621 cgaacgcgct ggtgatgatg acgcaggccg ccacgctgac cgacgaggac atcgccgaca
    10681 tcggtgcgta ctacgccgcg cagacgccca ccggcctcga agccgatccg tcctactgga
    10741 aggatggcga agcgctgtat cggggcggcg accacgagcg ccagatcccg gcctgcatgg
    10801 cctgccatgg cccggtgggc cgcggcaatc cggccgccgg ctatcccgcg gtgcgcgcgc
    10861 agcattccgt ctacaccgtt cagcagctga acgaatacgc cgatggctcg cgttacaagg
    10921 acctggcggg cggcacgcag aacacgcgga acgcccacat gatggtcact gtcgcgaagt
    10981 cgctgactgc ggaggacatc cgcaatctcg cctcgtacat ccagggcatg cgttgacccg
    11041 ggagctgccc atgattcgag tcctgctgtt cggcgcgctg ctcgcgtgcg ccgcgagcgc
    11101 ctgttcgcgc gatcccggca cgccgccggc cgcggaagca cccgccacgc aaaccgcagc
    11161 gccggcaccc gtcccggcca gtgagccagc cgcgcccgag gaagctgccg aagccgcgca
    11221 ggcgagccag gaaagcctcg gcgacagcga ggacgacaag tccgaccgcc cgctcgagcg
    11281 cctcgccgcg ctaccgcccg cggatgaact gcccggcggc aagtggaaag ccggcgtgaa
    11341 ctacaagccg atcgtgcctg gccagccgac gagtgcgccg ccgggcaagg tcgaggtcaa
    11401 cgagatcttc tggtacggct gcggccactg ctacgcgctc gaaccgttcg tccgcagctg
    11461 ggagaaatcg aaggccgact acatcgagtt cgtgcgcaca ccggtcatgt ggaacccggt
    11521 caacgtcgcg cacgcgcggt tgttctacac gctgcaggcg ctcaagcgtg acgacctgca
    11581 cgccaaggtg ttcgacgaga tccacatgcg cggcaacatg ctcgtcgcca acgacgacac
    11641 gcgcacgcgt cagatgcagc tcgccttcgc cagggcgaac ggcatcgtcg aggcggattt
    11701 cctgcgcgag tacgacggct tctacgtgac ctcgcagctg cagcgcgccg aagccgccac
    11761 gcgccgcttc caggtcgacg ccgtgccggt cgtattcgtc gccggcaagt actcgaccga
    11821 tgtcggtgca gccggtggcc agaaggaaat gctgcaactg atcgacgcgc tcgcggcgca
    11881 cgaaaaacgc cgctgaagcg cgtgccgcgg cagccgccag catgctgagc gcgttcctgc
    11941 ccggacctgc cgcactcacc cggctcgagc cgcactgcga cgcccctctc ccgcaggagg
    12001 cgctgtggtt cgacctgcgc gaacccaccc ccgaggacga gaggcgcatc gagacggcga
    12061 tcggcgtcgg catcccgacg cgcgaggaaa tgcgcgagat cgagtcctcg aaccggctct
    12121 acgaagaaga cggcgcgctg ttcatgaccg cgaccgtcgt caccaagctc gacaccgacc
    12181 tgcccgagag cacgcagatc accttcatcc tcgccggcaa gcggctcgtc acggtgcgct
    12241 atgtcgaccc gctgccgttt cgccgtttca tcgcctatgc cgagcgccac atgacgaact
    12301 gccactcggg cgccacgatt ttcgcgggcc tgctcgaggc gatcatcaac cgcatcgccg
    12361 atgtcatcga gcgcgccgga gccgcgctcg atgccgaatc ggccggcatc ttcgcgcaca
    12421 cggcacccgg caggcgcgcg acctcgcgcg acttccgtgg catcctcgag cgcctcggac
    12481 agaacggcga actgctgtcg aaatcgcgcg aaagcctcgt gtcgctcgga cgcctgctcg
    12541 cgtttgcgca gcagcaaccg gcgccgacct tcacgcccga tgtgcgctcg cgctttcgca
    12601 tgctgtcgcg cgacgtgctc gcgatgtcgg accatgtcag ctatctcgcg gccaaggtgc
    12661 agttcctgct cgaggcgact ctcggcatgg tcaacatcga gcagaacgcg atcatcaaga
    12721 tcttctcggt cgcggcagtc gtgttcctgc cacccaccct ggtcgcgagc atctacggca
    12781 tgaacttcca ccacatgccg gagctcgact ggctcgccgg ctacccgttc gcgatcggcc
    12841 tgatggtgct gtcggccgtg ctgccctacc tgtggttcaa acgcaagggc tggctctgac
    12901 cgccggcccc ggggggccac cgggcggccc ccgccccgct atacttgcag gtcgcagcgc
    12961 ccgtagctca gctggataga gtactgccct ccgaaggcag gggtcactgg ttcgaatcca
    13021 gtcgggcgcg ccaccttcaa agtgttgatc ggaaaggatt tcacaccttc gtgtgaatcc
    13081 ctctccagtc accaaactgg cttttttcgg ccatttggga cacttttggg acagttgtcg
    13141 aaacggatgg acctccgcat ctgcgtttcc acaacttttc gaggtccaca tcatggctac
    13201 ttacgaacgg cgcaaaggct tctggcgcgt caaggttcgt cgtgccggct ttcctcccca
    13261 gacacgcagc ttcaactcca aggcacttgc ccaatcatgg gcgcgcgacg tggaagcgaa
    13321 gcttgatcgc ggtgaactgg tggatcgaag cgaggcgcac gctcacacac tcggcgatgt
    13381 gctgaagcga tacctgaagg aagtatcgcc acacaagaag gggcatattc ccgagaccta
    13441 caaggtccgg tcgatactgc gatctccggt gtgccagctg cgaatgtcgg tactgagcag
    13501 cgctcacatt gctgcttacc gcgacgagcg ggcgcagtcg gtgaaaccgg cgacggtcaa
    13561 tcgcgacctg ggcatcatct cgcatgccat cgagaccgcg cgacgggagt ggagaatcca
    13621 catgccggac aatccggtgc atcgcgtgcg ccggttgcgc gcaccattga tgcgctcgcg
    13681 gcgattgtca ccggaagagg agcaacgcct gctggctgcc tgcgaggaat cacgcaagcc
    13741 gttcttgttg cccgtagtga aactggcgct ggagacggcc atgcgacgcg gggagatcac
    13801 gcgcgtcgag cgcaagcgga tcggcctcaa gcacaacgtg ctggtcttgt ccgataccaa
    13861 gaacggatcg gcacgggctg tgccgctttc caatgtcgcc aaggcgatca tcgagaaggt
    13921 gctggtggag atgcctccga cgccggatgg gttgctgttc gctggcgtga ccaacgaggc
    13981 gatcaagttg gcattccagc gtgcggtgaa gcgcgcgaag cttgatgact tccggttcca
    14041 tgacctgcgg catgaggcaa ccagccggct gcacgagaag gggctgaatc ccatcgaggt
    14101 catgtcggtg accgggcaca agtccatgtc gtcgatgctg cgctactcgc atgtgcgcgg
    14161 gctcgcagtg acggagaagc tgaaccggcc actggactac cagccgctcg tccaccagct
    14221 gacggagaac cgccatgaag gtcggtgacc gggcgcggtg ctcgacgcgc cggcgccgac
    14281 ggttgccggt accgctggcg cgggtcccga cggtttatcc ggtagcgacg ccccggaggg
    14341 ccaagcctaa aggcggagcc ttccgagcat tcctcaggcg gctgcgggac cggatgcacc
    14401 ggttcgtgtt cgtgccgggc agttgtgcgt ggatgtggcg cgatgggcag accaatccgg
    14461 ccatggggct cggcatgtac ggcggcgtag attcggcggg aaatccctac ggagtcggct
    14521 caatccacgt tgcgtcgcat cgtgactggc aggcagccta cgcatccgga atcagcagcc
    14581 tgccctgcgt cgtgatcgag cacgacagcg cgtggcgaga ctggtagcac cgattattaa
    14641 cactgtcttg cacgataaca tctttaatgt tatcgtgctt ttctatgtta gaaaaggcgc
    14701 cccccaaccc gctgaccgac gcagccatcc aacggtatct gcacgacacc ttggggctgc
    14761 ccaccagcct gcgcccgtgg tccggcgccg ggaaactccc gtatttcctc caggaagcct
    14821 ttgctacccg agagctgacg ctgcacggcc aaaccatcgt gctcgccgaa gaacagcggc
    14881 gccacaagcc gaagctggcg gaactgcgca cgcagatgga tcgcctgcgc gccgccgcgc
    14941 agcttccggt catctacgtc accgacgcgc tggcctctta cgaacgcaag cggctggtcg
    15001 agcagcgtgt gccgttcctc gtgccaggca accagttgta cctgcccgat cttggcatcg
    15061 acctgcggga gtatttccga cagcccacgc ccgcaatcga aaccggcctg agcccggcaa
    15121 cccaggccgt actcatcacc atgctgctga ccaaaccttg gcggcaggat tggacccttg
    15181 gcgagactgt ggcgcagctg ggctatacgc cgatgacctt gacgcgcgtg ctgcgcgaac
    15241 tgaccgccac gggactagcg aagacccggc gtcttggccg tgagcaccac ctctacacgg
    15301 atcagccgcc cgagaagctg tgggacagct tgcaagcgca gctgcgcaaa cccgtgaagc
    15361 gcagcatatg ggtcaagacc gccaagcagg aaaacaccgg cttgcgtctg gccggcatga
    15421 gcgcgctcgc gcgcctgacg cagatcgcgg acccaccttg gccggtgtat gccattagcc
    15481 cgaccgactg gcgtgcggcg aagcccctcg tccagttgct gaaggagccg gctgccgggt
    15541 gcagcgaatg gcagttgtgg acctacagcc cggcgctcgt gcccaacgcc cgcaccgtgg
    15601 acccgctgtc gctcactctg agccttgatc ccggcgccga cgagcgcatg cagatggcac
    15661 ttgatgaatt gcggaagcag ttcccatggt gagaggcctc gacctattcc ggcagttctt
    15721 tgccgcgcac gccgaccagt acgtgctgat cggaggcacg gctgcaacac ttgcgatgga
    15781 agcagcagga ttgccgtttc gcgccaccaa ggatcttgac gtggtcctgc acatcgaggc
    15841 gcttgatgcg aagttcggcg agtccatctg gaacttcatc gaagccggca ggtatgaaat
    15901 ccgccaggca agcgccaccg gaaaacccat cttctaccgc ttccagagac cggcggacga
    15961 gaactaccct gccatgctgg aactcttcag ccgcgcgccg gaaggcatcg cgcttgcacc
    16021 cggcagtcac ctgacgccca ttcccatcga cgaagccgtg gcgagcctct cggcgattct
    16081 gctggacaac gactactacg agttcgtgct cagcggacga cgcgatctcg atgggttgcc
    16141 ttgggtcgga gaagaccgcc tcattccgct gaaggcagtc gcctggctgg acatgaaagc
    16201 ccgcgcctct cgcggcgaaa ccgtcgatgc gagggatatc cgcaaacacg ccaatgatgt
    16261 gctgcgcctc agccagttgc ttgcaccaga taccaggatc aacgccacgg agaggattgg
    16321 ggcggacatc aacagattcc tcgacggcgt cctggccgag ggcgatatca atccggccga
    16381 cctcggcctg cgcggatcac tggcacaaat actcgcccgc gtccgcaacg cctacacatg
    16441 agcaagagcc gcgggaacga gccgggcagt ctcgccgcat tgcaggccga gaacatcagg
    16501 ctcattgcac tcctcgaatc ccgaggcatt ccgtggcaac ttccacaaga accaacgcca
    16561 gttgcagagg taacagaacc ctcccggctc accgccgatg agaaggtggc cctgtttcgg
    16621 cggctgttcc gcggccgccc cgatgtctat ccggtccgct gggaaagcaa gaccaccggc
    16681 aagtcagggt acgccccagc ctgcgcgaac gagtggcgac ccggcgtgtg cgacaagccg
    16741 cgtatccgat gcgccgactg caacttccgc agcctgactc cggtatccga ttccatcatc
    16801 tacagccatc tcgccggcaa gcacactatc ggtgtctacc cgctattgga ggacgacacc
    16861 tgccattttt tggctgtgga cttcgacgaa gcgacatggc gggaagacgc caaggccttc
    16921 cgcgtatcgt gtgcagaact tggagtgccg gtcgccctgg agatttcccg ttccggcaat
    16981 ggcgcacacg cctgggtctt cttctccggg agggtgcccg cacgcgatgc acgccgcctg
    17041 ggaaccgcca ttatcagcca tacctgcgca cgcacgcgtc aacttgcgct cacttcctac
    17101 gaccgcctgt tccccaacca ggacaccatg cccaagggcg ggttcgggaa cctcatcgcc
    17161 ctgccactgc agaagtaccc gcgtgaacaa ggctgcagcg tcttcgtcga cgacacgctg
    17221 caaccctaca ctgatcagtg gggatatctc gcaaccgtgc agccgatggc accgcatgac
    17281 atcgactcga cgatcctgcg cgccacgggc aacaaccatc ccctcgatgt gaggttcatc
    17341 gaagacgagg acctggctgc gccctggaag tcccggcccg ccccttcccg gcgcctgccg
    17401 gacaagatgc cggaatcgct gacagtgact ctggcgaacc agatctactt cgagaaggcg
    17461 aaactgccac aggcgctggc aaacaggctt atccggttgg ctgccttcca gaacccggag
    17521 ttctatcgtg cccaggccat gcgcatgtcg gtttgggaca agccacgggt catcggttgc
    17581 gcggagaact tcccgcagca cattgccctg ccgcgcggtt gtctcgatgt cgtcagcgaa
    17641 ttgcttgccg agaacagcat tcgtgttgct ctcatcgatg agcggtattc ggacaagcca
    17701 atcggggccg agttcacggg aacacttcgc gccgatcagg aggctgcggc cgcggcgatg
    17761 ctcccgcatg atgctggcgt gctatgtgct ccgacggcat tcggcaagac ggtcactgcc
    17821 gcctccctga tcgctcgccg cggtgtgaac acactgatac tggtgcaccg gacggaactg
    17881 atgcggcagt ggcaggaacg tttgagcgcc ttcctgaatg ccgctggaac accggtgggc
    17941 gtcatcggcg gggggaagaa ccgcccaacc ggcgtcatag atatcgccgt catgcagtcg
    18001 ctgtctcgaa gcggcgaggt agacccgctt gtcgagggct atggccacat catcgtcgac
    18061 gagtgccatc acgttggcgc agtatcgttc gacgccatcc tgcgccgcgc ccgcgcgaag
    18121 ttcgtcctgg gccttaccgc gacaccgatc cgacgggacg ggcagcagcc gatcatcttc
    18181 atgcaatgcg ggccggtacg tcatacagcc agccggccag cgggtgcccc tgccgacctg
    18241 gaggttgtgc ctcgctctat ctacgcgcct cttgatgttc cagccgatgc tggcatccag
    18301 gacgtatttc ggcaccttgc cggtgaccag accagaactg cggccatcgc tgcggaaatc
    18361 cgatctgccg ccgaccaggg ccgaaagatt ctggtactga ccgaacgggc ggatcacatt
    18421 gatgccctcc gcgcggcatg cggcgacaga gaaccggcgc cgttcgtact gcacggcagg
    18481 atgtccagga agctgcgatc gacgttgatt gcacagctca acgccctgcc gccggacgcg
    18541 ccgcgaatcc tgctggcaac aggcaggctg gttggtgagg gattcgatca cccgcccctc
    18601 gatacccttg ttctcgccat gccggtttcc tggaaaggca cacttcagca gtacgccgga
    18661 cgactccatc gagagcatgt cggcaaggcc aatgtgcgca tcatcgattt cgtcgatgcc
    18721 ggtcatcccg tgttacgacg catgtgggac aaacggcaac gcggctacaa agccatggga
    18781 taccggatcg ccacggaagg cctgtttgac cggcagcatc agttcaagga gtgaattccg
    18841 cggtctcctt cggtgctatc gacttcgtgc catacccatg gaccacagca tagaccgcgc
    18901 cagccttgcc ctcggtagta ggcacgtccg gctcgatgcg cacgcacagc cgctgcgagc
    18961 cattcacctc gcattgccgg aattcggcct tggatagctc ccgcaatcgc tcatagcccg
    19021 cggcctggac acgcatcagc tctccggctg tggccgccat cgatcggctt ccccaccaca
    19081 tcgacaacgg gaacagcaac gcgatcgtcc ccagcgcgat caggccggca tgtcccagcg
    19141 tcttccactg cagcgaactg ctgatcgact ccgcccgatt cacaactgcc tgcacacgtc
    19201 ccatcaactg atccagcccg ccttccacca cgtgcagtcg ggtctccacc gtggcaaccg
    19261 ttgccttgct gcacgcgcgc gcctgcgcca gcgcgatctc ctccgcttcc gccttgatgt
    19321 acttgggcca gcggtgccgg atgtcagcaa gcaactttgc gtggtcgaga ttggtcgtgt
    19381 gggtctccct gcccagcttc tccagctgct gcagggccac atcgtgcttg tgatgcgctt
    19441 cgtccgcgat ccgccgcgcc cgcgcggctt cttcattcaa tgcacggata aactctttga
    19501 gcgagctgcg cctcctcctt cgaactgaac gcgccagcaa caaggtaggt ctgcgtgcag
    19561 actgatccag gctcaccaac tatcgacggg ccaagcacca aatccacacc gcttctctct
    19621 cgctcccgtc ccgaccccgc caccggaagg aacagcttcc atacatcaat catctgtgtg
    19681 ttcttgctga tgcccttgcg agccgtggcg ccacgcactc ttcgagtgcc aacatttgca
    19741 tacaagcgaa cctgacccgc cttcggcggc ttatcctgca catagtccgc gaagttagtg
    19801 gcaagtccaa atggtgtgtc cccactgaca aggtcctcaa acgacccctc ctccctcgtc
    19861 aacaccttgc gcaaaatatc cactgcccgc ttgtcgcgta cgaatacatc gaactcatcg
    19921 aggtcccgcg cgtgaggccc aatcgtggcg ccatttcgat ggtagattga ctcgcatcgc
    19981 cccggattgt cgcgatccca aaggaagtaa caaatcccac cacctattcc aacggtggga
    20041 aaggcatcct tagcattctc atagtcaaca agcatgcgca aacgtctatc gcccagaaat
    20101 tcggagcgga aatcgccaag cccacgtcct ccggccatcc atcgggatgg aatgaccatg
    20161 ctaaggaacc gtggctccaa ctgcatggct tgccgaacga acatttggta aatcggcgaa
    20221 tcattcgttc cgccgcctcc gcctgtcatc tgatacgggg ggtttcccac gatgacgtcg
    20281 aactgcatat cacctccgaa aagcccagca atccagccct tgatgtcgtt ggtgtgaatg
    20341 aacgcgtacg catgcgtttc tagcgcatcc ccgcgatcca gcgccttctt gctcgctccg
    20401 caatacgtgc accgaccttc catccacaca tgttcggtac gctcgaacca gacatttccc
    20461 gcatcactag cgaagccttt agcgattgaa tggtcgccct gtgcatgttt cgagcaatac
    20521 acgctccgcc gcgccagcat gcctgtgaga tgcgtgatac cgatgccaaa cacctgcttc
    20581 gtcaggatgt gattgacgcg ctcattgaga tccggaatct cgttggtcag cccaattgtg
    20641 aggcgcttgg caatctcacg gaggaataca cccgactttg tgcaaggatc aaggaacctt
    20701 acgtttctgt ccgcccatat gtttgcgccg ttgtggccgg ctgaccaagc ggccgccagt
    20761 gcattcagca tctggttagc aaactcaggc ggcgtgaaaa cctcatcatt ggaaagattc
    20821 gctatgcatg tcagcacgtc cggattgcgt ccgcgcagcg cgaacgatac ctgagtgttc
    20881 atacggccct cttccgttct actccagttg cttctgcagc caactcacgt gacgtcacga
    20941 gcggatacgt cttcaccggc gtgaagattt cgtgcttgcc caagtgggcg aagagcgagc
    21001 cctccgcact gaatgcagac gaacccaata ggacatcaaa gcgaaagtcg cgtcgctgga
    21061 accggccttt tccaaggtaa ccccattcag caaacgtgat tggtttcccc tcgtgattac
    21121 acatcgtgag tgcatcccca tgaactaggt tctgcgatag cacgaagctt gcagcacgat
    21181 agaggtcgtc cgacgcggac agattcaggt actccgcgag aatttccagc aggttcgcgc
    21241 ggcactcggc aatattgtcc gccagcagct cgataccgta gatacacatc aacccaagca
    21301 gtgcatagtg ccggcgctca aagtccgact tgccgtactt gagttcgacg gccgccagct
    21361 tgcgtcgcaa gacctggacc aggaagttgc cgctcccgca ggccggctcc aggaaacggg
    21421 agtcgatgcg ttcggactct tccttcacca gatccagcat tgcctcgacc agccacgccg
    21481 gggtgaagac ctctccgtgg tcggcgacgc gttgttttga cttcacgagg ctcatgattc
    21541 agcggatgtt agcaattcaa gtgccgctac cggcgcgatg atctggaact tcacctgacc
    21601 attgaggctc aacaaatcct gatcgccagt caccagccat tgcgcaccgc cgactgcagc
    21661 gaggtcgagg aagatctgat cacattcgtc acggcattcc ggcactgcgg gtttggcctg
    21721 ccacggcgca acgacttctc catatggcag gtagtccgcc aacaactcat cgcgttcctc
    21781 cggcgtgagc ttgaacttgg ggtaagccag cacccgcagc aactcttcca cggtctgcgc
    21841 gcacaacagc ggcgtcaggt cgccggactg ccaggccgcg cgcaacggca ccagtcgacc
    21901 attgacgaac agcaatgccg acaggaccgt gttggtgtcg agtacaaccc tccgggcctc
    21961 agccatcaac gctggctgcc cgcgctacgt gcccaggtga cagcgtcctc tacgtcctgt
    22021 tccgaaatct gaagttccgc cagctttgcg cgcaccgcgt cagcccgctg tatccgcacc
    22081 ggggtcaaaa cgatttgccc gtcttccgtc cgaacctcgt agtaccgggg gtcgccgaca
    22141 gccttagtga tggccttcgg cagggtcagt tggttcttgg cggtcatctt ggccagcatg
    22201 ggcttcttcc ggaggaaaga aaggaatcct tactctacca gaatcgctgc ctcatgcaag
    22261 catgccgctc agagggttcg gagagatctg tacagctgca tagacatccc atgggatgtc
    22321 acatagcatc agggcgatgc gagtggttct ggacacgtcg gtccttgtcg ccggcctcag
    22381 aagccagctg ggtgccagca atctgatact gatggccgct gcggaaggga catttcgtcc
    22441 tttggcatca actgcggtat tcctcgaata cgaggccgtc ctgatgcggg ccgaacagcg
    22501 cttggccacc gggatgagtg cggaggatgt ccagggattc cttgcagctt tcgcgagcgc
    22561 ggctgacgcc gtggaaatcg acttcagctg gcgcccgcaa ctgcgcgacc cgtcggatga
    22621 gctggtcctg gaggctgcgg tgaatggaca ggcaaccttt atcgtcactc acaacgtggc
    22681 tgacttcgaa ggtggcgcag cgcgatttgg tattcaaaca ctgacacctg ggcagttcct
    22741 caaggagcta agaaagccat gagcaagagc acgtacccac tgaaacttcc gaactcgatc
    22801 aaggacgccg ccgccgagct ggcacggctc gatggcgttt ccctgaatca gttcatcgcc
    22861 gccgccgtcg cggaaaaggt tggcgtgctg cgtacggcga gcgagttcct gaaacgaagg
    22921 gctggcagtg ccacgccgca agacatggtc cgctatctgc gtgcggctcc gaagctgcct
    22981 ccgatggaaa gggatgagat cggctgacgg gactgctgca tgcatctgcc aaattgtggt
    23041 ggacaaattt gggacagttc gaaagaactt gagcaggaat atgcgggaat cagtgggaca
    23101 ctaccgcctt gattccaaag aaattcccgc gtaacgtatt gatcggaatc catctttttc
    23161 ggctctccga aggcaggggt cactggttcg aatccagtcg ggcgcgccac cttggacttc
    23221 atcgcgcgag cagcgcctcg atcgagcgca tcggcaaccc gaggcgcagg gattccggca
    23281 gaggcaggaa gccgaaggat tcgtagaacg cggcagcgcg ctcatgcaga gcatcgacga
    23341 gccgacaggc tcggcgctgg cgcaggcgca ctggaccgca tccgcgagca gcagcgagca
    23401 gcagcgaact gatgcgcccg ccgccgctgg cgttgtaaga cgccgaccca gcacgcactg
    23461 cttgacgatg tggacgacgc cacgcctgct gcgccgcgtg gccggagcgg cgcaagcgat
    23521 cagcaccgct tcgattttca gcggcgcggc gcgatcgtcg tcaccagcgt ttcgaggcca
    23581 tcgataccgc cctcgatgcg gtctcccggc tgcagcggtc cgacacccgc cggcgttccg
    23641 gtgtagatca gatcgcccgg tgcgagctcg aacaatgtcg acagttcggc aatgatctcg
    23701 ggcacgctcc agatgagttc cgaaagatcg gcgttctggc gcatctcgcc gttgaccgcg
    23761 agccagatcc gaccggaacc gaggtggcca ctttgggttg ccggcgtgat cgcggagatc
    23821 gccgccgaac ggtcgaaccc cttgcccgtg tcccagggcc ggcccttgtc gcgcgcttcg
    23881 aactgcaagt cgcggcgtgt caggtcgttg cccacggcat agccgtacac atgcccgagt
    23941 gactgcgcgg gcgcgatatc gcgcccgccc ttcccgagtg cgaccaccgg ctcgacttcg
    24001 tgatgcaggt tcgcggtgcg cgacggaagg ggaatcgtgg cgtgattcgg cgtgatcgca
    24061 tcggccggct tggtgaagaa gaagggcggc tcgcgttcgg gatccatacc catctcgcgc
    24121 gcatgggccg cgtagttgcg acccacgcag tagatccgat ggaccggaaa gcgcgcgtcg
    24181 ctgtccttga tcccgaccga gggttgcggc ggcggcggca agacgaacga catcaaagtc
    24241 tcctgttggc tacttgcagt gctcgggacc gatcgccgag acgggaacaa ccgtcagcgt
    24301 ccaaacaggc gccccgcaat gttacatcgg cactggcagt cagttcgtcc gcggacttgc
    24361 caacggcgat gcaaggcact cggcctttca cagcttcagt ttgcagatgc gtttggcatc
    24421 agcgcctcga cgctgctcag gatcattggg cgtgaaccca aagcggccac ggatcactgc
    24481 cgcagccacg cgcgcggcga gacgcccacc gcctgcgtga acgcgcgcga cagcgacgag
    24541 gcgctggcgt agccgagctg gtcgcccacg ctcttgatcg gcgcgccatc gcgcagcagc
    24601 gtctgcgcga tcgacacacg ccacttgagc agatactccg ccggggtctg gccgacccat
    24661 tccctgaacg ccgtggcgaa cgcgctgcgc gacatgccgg ccgtgtcggc catcgactgc
    24721 agcgaccagg gcgcgcccgg ctgctcgtgc agcgcggtca gcgtgcgggc gagcttcgga
    24781 tgcgacagcc cggtgagcag gcctgcgggc agccctgcct cggccggatg atccagcagc
    24841 cagcgcagca gttgcacgag caggacttcg aacaggcgat cggcgagcag ccgctggccg
    24901 cagcgcacgc gctcggcctc cgcgaacagc agctcgagcg tgtgttccat gccctgcacg
    24961 gacttgagcg gcagcgcgag cagcggcggc agcgcgcgca ccagcgggtg ggaggcgccg
    25021 ccgtcgaagt ccagagccgc gcaaacgagg ttcggtccct cacccggcgc atgcacgaat
    25081 tcatgggtca cggggcgcgg gtagaagagc agcgtgggtt cggtgagctc gatacggcgc
    25141 ggcgcgccgc tgcccgcacg atgcttcacc accagttcgc cctcacgcaa cacgtgcagg
    25201 aagccgcgcc ccggctcggc gggcaagttc gtcctgccgc agagcgcgcc ggcgtggaac
    25261 aggcgggcgt gcaccctgaa gcgcgcgagc agcgcggaca gccggtcgat cgggcggatt
    25321 tgctcggcac tcactgtacg aattgatatg ttttatgaac gattggtgac caatcgtatc
    25381 gagtgcggcg ggacactgtc caccccggga caacgcccga ttcgacggag cagaaccatg
    25441 aaccgcatcc ccctcatcga ccgcaacgcc acctcccccg accgcaaggc gctgctcgac
    25501 acgatcaatg gtgccttcgg catgacaccc aacatgttcc gcgctgtcgc caattcacca
    25561 gccgcgctca agagcatgtg gggttcgttc ggcgcactcg gcgcgggcgt catcccggcc
    25621 aagctcggcg agcagattgc cgtggcggtc gcgaaccgca acggctgcga gtactgcctc
    25681 gcggcgcata ccgcgctcgg gcgcagtgcc ggggcgagcg ccaccgagct gcgcgacgcg
    25741 caggccggag tttccgccga tgccaggacc caggcggccc tgcacttcgc cctcaagctg
    25801 gtcgacaagc gcggtcagat cgaagctgcc gacgtgcagg cgctgcgcac agccggtttc
    25861 ggcgacgcgg agatcgtcga gatcctggcc cacgtcgcct tgagcctgtt caccaactat
    25921 gtgaacgtcg ccctcgccgt gccggtggat ttccccggcg tgaaactgcg cgaagcggcc
    25981 tgacgatccg caggagcagc cctcgacatg actgacgttg ccgcgccggc cctgcaggtc
    26041 agtgactggc tcaacgcacc ccgggacttc agcctggccg cgctacgcgg ccaggtggtc
    26101 ctgctgcatg cgttccagat gctgtgcccc ggctgtgtcg cccacggcat tccacaggcg
    26161 cagcgcgtgc accggctgtt cggcaccgag gcgctcgcgg tcatcgggct gcacaccgtg
    26221 ttcgagcatc acgaagtgat gaacgccgcg gcgctgcgcg tattcgtgca cgagtatcac
    26281 ctggcgtttc cggtgggcat cgatcaaccc ggcgatggcg acccggttcc gctgacgatg
    26341 cgcgcctacg ggtttcgcgg cacgccgagc ctcgcgctga tcgaccggca gggcaggctg
    26401 cgcttgcagc acttcgggca gatcgacgac ctgcagctcg gcgcggcgat tggccgtctg
    26461 ctggccgagc cggccccgcc cgcggcgcca taggtcctct atcgcctgac cgggccgcac
    26521 tgcccagcat gccgaccggc actggattca gggcgcgcaa tcctccgcca cgcgccgcag
    26581 cccatcggga tcgcgtatcg tgagatagcc gttgcggctc gcgagcagcc ccgccttctc
    26641 ccacgccgcg agcgtgcggc tcgccgtatg cagcgtggtc ccggagaatt ccgcgatgtc
    26701 cttgcggcgc agcggaaagg cgatcgtcgt gccgcccgag ccgtcccgcc cggcctgcgc
    26761 cgcgagccgc agcacggcgt gcgcgatccg ctgctcgaca cgctgcgtcg tcagttcacg
    26821 caggcgctcc tgcaactggc cgagccgcgc accgagcacc tcgatcacgt tcatcgcgat
    26881 gggcgggaag agcgcgatca gccgccgcag ttccgcctcg ctccagctga gcacgaccga
    26941 atcctcggcc gcgatcgcat cggccggcag gcgatgatcg gtgaacagcg gcacggtgcc
    27001 gaaggtttct cctggcgcaa tgaagcgcac gatcgcctgc ccgccatcgc tgccgatctg
    27061 catgatgcga acgctgccgg cgccgagcgc gtaagcccgt tccgcgcggt cgccctgcac
    27121 aaagatgacg tcgtccttcg cgacgcgatc gacgcgcatc gcggcgcgca cggcgtcgag
    27181 ggccggctcg gagagtgaac ggaacagctc gagatttccg ggttgccagg gcgacccctc
    27241 gggcggcgcg aacgcgcgag cgcccatttc gacgctccgg gcatgtgtca tgaccaagat
    27301 cgactccatt tgtgctgcga caaagacggc tttgtagtac tggagtatat gagaatgact
    27361 atcactcgca attgatggcg tccgcaacat gcctgccaac tacccccgca aaccccgggc
    27421 cgggctggcg ctcgctgcgc tgctcctgtc ggccggcctc gcgcccagcg gtgtgtccgc
    27481 ccagtcggcg acaccgggcg ccgtcctcga cgaggtcgtc gtcagcgcat cgcgcgatcg
    27541 acccgtagtc cgcctcgatg ccgcggcggg tggcacggcg atcgtcacct cgcgcgagat
    27601 gccggcgtcg acccatctca cgctctcgcg cgccgtcgcc gacgtgccgg gcgtcgtggt
    27661 acaggatttc ttcggcggca acgaccagcc gcgcgtgcag atccgcggat cggggctgca
    27721 gcagaatccg gtcgagcgcg gcatcctgat gctgcgcaac ggcctgcccc tcaaccgggc
    27781 cgacggctct tacgtggtcg gcttcgccga tcccggcgcg gcggatgcaa tcgagatctt
    27841 tcgcggctac atggccaacc ggctcggcgc aaccgtgctt ggcggcgcgt tcaacctgat
    27901 atcgccgacg ggacgcagcg cgcccggctt cgcgctcgcg gccggcggcg gcagcttcga
    27961 cctgctcggc ggcaacgcgc agtacgggta cgcacacgag cacatcgatc tgctgctgca
    28021 gggcggctac acggagcgcg agggcttccg tgactacaac gcatcccgcc gcgggaactt
    28081 cggtggcaat ctcggcctgc gcctctccga cacgctcacc gtgcgcctct tcgcgggcca
    28141 tgtggatctc agcttcgatg tcgccggtcc gctcacgcgc gaacttctcg agcgcgatcc
    28201 gcgcgccgtg ttcaccggcc cgagcgtggt gcccggcggc gcgatcaacc ccggcccgaa
    28261 cgtcgtgcgt gaccgcccgc gccgcgacgc ctcgcaattc caggccggcg cgcgcgcgac
    28321 gaacacgtac ggcgcacatg tcgtcgattt cgcgatcggt cgcgcacgca ccgacgacag
    28381 cttccgcttt ccgatggccg cgggcatccg cgtgaccgag ggcgacgaca cgaccgcagt
    28441 gctgcgctac gcgttcaagc cggacgccac gcgcgtgctg ccgctgttcg aggcgaccgc
    28501 gcaatacgtg accggtgcgg ccgatcgcga caaccatctc aatctctcgg gccggcgcgg
    28561 cgcgctgttc ggctcgagcc gcctcgaagc cgacacgctg tctctcaacg cgggtctcaa
    28621 catcgcgctg acgggccggc ttagcctgtc gccgtcggtg tcgtggtcgc acgcgagccg
    28681 cgacaacgac gaccgttacg atctgccgac gcgcccgacc gccgcgtaca gcccggccga
    28741 tcccgccgtc gcgctgccga atggcgcggt gcccaccgtc gacagcagct acgcgcgtcg
    28801 ctacaaggcc tggagccccg cgctcggact cacctggcag ccgtcgggtg accagaccct
    28861 gtacctcgca gtgagccgaa gtttcgagcc gccgacccat gacgatctgc tcgcgaccgt
    28921 caacggcacg ccgaacagca gcgccgggcg cccgaatccc gccgccccgg cgcagccggc
    28981 cgccgcattc gtcacgcctg ccctcgaagc gcaacgcgcg acgaccgtcg aggcgggctg
    29041 gcgcggcgac tccgggccga tgtcctggga cgcggccgtc taccattcgt gggtccgcga
    29101 cgagctgctg agcctgcgtg acgaatccgg cgtctcgctg ggcgccgcca atgcgccgag
    29161 aacgcgtcac ctcggcgtcg aactcgggct cacggcgcgg ctcgccagca cgttcaccgg
    29221 ccgtatcgtc tacacgtacc aggacttccg cttccgcgac gatccggtac gcggcgacaa
    29281 ccggctcgcc ggcgcgccgc gtcactggat ccacggcaag ctcagctggc agccggccgc
    29341 gaactggaat gcggaggccg cggcgcgctg gatgcccggg gagacgccgg tcgacaatct
    29401 cggcacgctc tacaacgatg ccttcgcgac cgtcgatctg cgtgtcgaac gcgtattcgg
    29461 ccgcgcgctg tcggtgttcg ccgaggtgac caatctcctc gacgagactc atgctgcctc
    29521 ggcgctgatc gtcgatcagg cgcgatccga ccaggcggca ttccttcccg gtgacggtcg
    29581 cgccgtgggc gccggcatgc gtctgagatt ctgattcctg atcctgattc tgcatcgagg
    29641 taccaccatg agacatcgca ccgtactgct cgccgctctc gcctcactga tcgtgccggg
    29701 catggccgct gccgattcga ccctgctcga actcgagcgt ctgaactacg agaccgttta
    29761 cgttgccgcc cggcgcaccg tgctcgcgct cgacagcgaa ggacgcccgc tgaccgcgtt
    29821 gctgcgcatc gccaagggcc agttcctgcc accacatggc gccgagggtg gccttcgcgt
    29881 gctgacggtg ctgtcgggca cgctgtcctg gggcgacggc gccaaagtcg atctggcggc
    29941 cgagcgcacg ttcggccccg gcagcgtgat cgtcgtgccc gccgccggcg gcgggcactg
    30001 ggcggcagcg aggcaggacg acgtactgct gcagatcgtg atggtccggc agggcaagct
    30061 ggctcccgag gccgccgcgc aactggagtg atgatggaac tggccctcgc ccgcgcactg
    30121 catgtcctcg gtgtcgtcgt ctggatcggc ggcgtgtcga tggccacctc ggtcgcgctg
    30181 cccgcgatcc ggcgcggcgt cttcggtgcc gaccgcctgc aagccttcca tgctttcgaa
    30241 ggtcgcttca tctggcaggc gcgtaccgcg gtcgtcatcg tcggactcac cggcttctac
    30301 atgaccgagc gcctcggcct ctggtggcgc ttcggcgacg ggcactactg gtggatgcat
    30361 gcgatgctga tcgtgtggtc gctgttcatg ctgttgctgt tcattggcga gccgttcgtc
    30421 ctgcaccgct tcttccccgt ctgggtgaag cgcgatcccg ggcgagcctt cgcgttcctg
    30481 cacacggtgc acgtcgtcct gctcgtgctg agcctcgtca cggtgttcgg cgccgtcgcc
    30541 ggcagtcacg gctggatgcc gtagcgcacg atcccgttcc cgccgctcag cgccgctgta
    30601 cgggcttcgc ggaacgcgcg gcggtcggcg gcggtccggg ataggcgcat tcggcgatcg
    30661 tggtc
//
LOCUS       DAOQVY010000015        30598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_1757_length_30598_cov_5.800805, whole genome shotgun sequence.
ACCESSION   DAOQVY010000015 DAOQVY010000000
VERSION     DAOQVY010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 30598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30598
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1757_length_30598_cov_5.800805"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..2265)
                     /gene="glgB"
                     /locus_tag="PKN91_02710"
     CDS             complement(82..2265)
                     /gene="glgB"
                     /locus_tag="PKN91_02710"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HNS27012.1"
                     /translation="MSNPDGLLDTLAHDLGRLADGRHHHPHGVLGPHREGTRLAVLLY
                     LPRAHDVMLEDTLTVPRVGQTDFFLWQGDAAHVPERYRLRWRDAHGAHSRHDPYAFAP
                     ELEPAALERFGRGAHAAAWELLGAVPRVRDGVAGMRFAVWAPNAERVSVVGPFCNWDG
                     RQYPLSARGSSGVFELFVPDLAPGELYKFEIRNRDSGTLSLKSDPYARRAELRPATAS
                     RLPAATSYAWQDEAWLRARAARDWLHAPMSIYEVHLGSWQRHADGGFPGYRELADTLV
                     PYVRDLGYTHLELLPVTEHPLDDSWGYQTTGFFAPTSRHGEPDDLRHFIDRCHAAGLG
                     VILDWVPGHFPRDAHGLARFDGAPLYEYADPRRGEHRDWGTLVFDYGRNEVRSFLLSS
                     ACWWLEEYHFDGLRVDAVASMLYLDFSRKDDFLPNRYGGNHNLEAIDFLRELNALVHA
                     RCPGAVVIAEESTDWPMVSRPAHDGGLGFSMKWNMGWMHDTLAYFRQDPLFRSYHHQR
                     LTFAMMYAYTENFVLPLSHDEVVHLKRSLLGRMPGDRWQQLANLRLLYLYMWTLPGKK
                     LLFMGGEFAQDSEWNFAAALPWSLADDPAHAGVRQLVRDLNRLYVGDPSLHRYDFEPR
                     GFQWLDCDDAASSILVYVRRSDEHFVVVALNFTPVPRIALRIGVPEAGSYREILNSDS
                     QYYGGSNLGNPLPLPATPTPHRGQPCSLEITLPPLGGVVLRRDAS"
     gene            2322..3596
                     /gene="glgC"
                     /locus_tag="PKN91_02715"
     CDS             2322..3596
                     /gene="glgC"
                     /locus_tag="PKN91_02715"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNS27013.1"
                     /translation="MTKQAARASSGRYVSRLTSGALAVIMAGGRGERLGPLTESRCKP
                     AAPFGGKFLIIDFVLSNCVNSGIRQIMVLTQYKAQSLIHHVQNGWNYLRGEFGEFVDV
                     IPAQQQIGMHWYRGTADSVYQNLDAILQLRPKYVLVLAGDHIYKMDYGPMIAYHVEKG
                     ADITVGVVEVPRASAANAFGVLTVTESNRVTRFTEKPADPDPVPGKPDSALASMGIYV
                     FSTRLLEQLLVADAADPDSKHDFGRNIIPGAIPDMQVFAYPFQDVKTRAQNYWRDVGT
                     IDAYYEANLELVHVAPELNIYDEEWPIWTYQVQQPPAKFVLNDGARVGTAINSMIAGG
                     SIVSGARVTESLLFSNVRVNEYSTVHRSLVLPGVNIGKDCHINNAILDEGCDVPDGAR
                     IGIDTAADRERYFVTEKGVVLVTAAMLRAAGA"
     gene            3601..>3859
                     /locus_tag="PKN91_02720"
     CDS             3601..>3859
                     /locus_tag="PKN91_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HNS27014.1"
                     /translation="MSGRLPVVLLWHMHQPQYCDALTGEYTQPWTALHALKDYSDMAA
                     HLEDNPAARAVVNFTPVLIEQLEALALQVRAHLGIGAALLDP"
     assembly_gap    3860..3869
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3870..5274
                     /locus_tag="PKN91_02725"
     CDS             <3870..5274
                     /locus_tag="PKN91_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNS27015.1"
                     /translation="PEARLALVRALLRAQRAQMIEPLPAYRELVDIAALLATPARIAY
                     VSDAFIRDLSVWYHLAWTGETIRRRDARVAALMARARDFTTDDRRALLAVIGDTLASI
                     IPRYRALAAAGRCELSVSPYSHPILPLLCDFAAARESLPASPLPRHAGYAGGRERADW
                     HMGEAVRVFERAFGARPVGCWPSEGAISHAALEVIDRHGFAWVASSAGVLRASLDARS
                     GEVAWAADALDRPYRLPGQRLQCWFRDDGLSDLVGFTYSTWHGDDAVAHLVAALVELA
                     ARHGDSPGHAVLIALDGENAWEHYPQNGYHFLRALYAALATHPQLELTTLGALSARES
                     LPPALLPHVRAGSWVHSTLATWIGDADKNAAWDLLCEAKLAFDRVVAGGALVGESLAA
                     ARRQLAQCESSDWFWWFGDYNPAEAVAQFDLLFRRQLTVLYRMLALEPPAELGQPVSR
                     GGGSMEHGGVMRRAHDD"
     gene            5264..6631
                     /gene="malQ"
                     /locus_tag="PKN91_02730"
     CDS             5264..6631
                     /gene="malQ"
                     /locus_tag="PKN91_02730"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNS27016.1"
                     /translation="MTTESPRRAGVLLPMFALPRAAAGAVAARAFVDWLAAAGFTVWQ
                     VLPIGPTGADGSPYWLRSDHATHIALIDRQDYSADDYHAWRERERDWIDDYALFEVLA
                     AAHGGAPWQEWPVALRDREPAALAAARASHAEAIGRIVLDQYLAARGWSELREYANSR
                     GVALFGDLPVYVAPDSVETWVHRRQFQLAAAGRPTALAGVPPDYFAADGQLWGNPVYD
                     WDAMAADGYAFWCARVRRALSRYDLLRLDHFRGLVAYWRVPAGAATARDGSWQPAGGR
                     PLLHALQALAQDRPHPGSSTYVAEDLGVITPDVEALRCEFRLPGMKVLQFAFDGSAAN
                     PYLPHRHMRESVVYTGTHDNDTTLGWYQGLDGHTRARVDYVLRVHAGEMPWALVHAAL
                     ASVADLAMLPVADVLGLGGAARLNTPGTTVGNWQWRLPPGALTPQLAAQFRHANQVFG
                     RALLG"
     gene            6637..7113
                     /locus_tag="PKN91_02735"
     CDS             6637..7113
                     /locus_tag="PKN91_02735"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HNS27017.1"
                     /translation="MRILIATALAVLLSSCAATRLETPHLQLVNIEMMESELFEQRFK
                     VRMRVQNPNDRALPVQGISAKMQLQGEDFAQGVAGEAFTVPAFGEAEFDMVLRANVAG
                     AILRLLSSMKDKKSPDKLDYRVVGKLSLSSGFLRSVPFEESGSFNLGELTKGSGRP"
     gene            complement(7130..8536)
                     /locus_tag="PKN91_02740"
     CDS             complement(7130..8536)
                     /locus_tag="PKN91_02740"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HNS27018.1"
                     /translation="MPLRIALLASEVAPFSKTGGLADVSAALAKHLTAAGHEVVTVTP
                     LYSSIDRAAHGLSTSGGITTARLPGSPATVYFIDEPRLYDRATLYTADPDEHLRFIRL
                     TRAAFELCRRLGFAPDILHCNDWHTAFGPLLLAAEYGADPLFAAARSVLTIHNIGYQG
                     SFDAEYAGDIGLDDLSRLHQEDLRAGHVNALKHGILYAHAVTTVSPTYAREICTEEYG
                     MGLQETLRARGDSLTGILNGVDYDEWDPRHDRWLPIHYDAQRLGAKAELKRTFLAREG
                     LPFRLGVPLAGVVSRLAEQKGIELLFDSLPPLLAARRLQLVALGSGEARYEQFFARLA
                     RDFPQEVRFHAGYDEARAHWIEAASDMFLMPSRYEPCGLNQMYSLRYGTVPIVRRTGG
                     LADSVEHFDAATGRGTGIVFNDFDSAGMAWALQQALTLHADEPAWTRLMQNGMAQDFS
                     WTRQSARYVALYERVLGR"
     gene            8594..9673
                     /locus_tag="PKN91_02745"
     CDS             8594..9673
                     /locus_tag="PKN91_02745"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNS27019.1"
                     /translation="MLRRLSLVLVTSGALLVSAGIGVLAADWPFLHRLWQLPADVSGW
                     RETVAVPTVTIAGSDAPYIPPAAPDPLGIDAALLEQAASQAEAEDSVALLVLHRGALL
                     IERYWHGITRDTLYPLEGLTRALLGIAYGRALDDGSIESLDAPIEQWLPEWYGEPRGA
                     ITIRQLLWNVSGLETPAEEGWWGRLGKAGRLRFGTTFERTALSYRAAHEPGTRFELSR
                     VDAQLAALVLERASDEDYAGFIERSVWQPARAGRAEFTLDRPHGAAAAFDGLRATPID
                     VLKIGALLALDSGWAGEMARGSLPNPRYGLQASAVDGDGLLLSGDGRAVWVWPGEQLV
                     IVRLGDAAPGWDASAWAQALRRSLH"
     gene            9673..10536
                     /locus_tag="PKN91_02750"
     CDS             9673..10536
                     /locus_tag="PKN91_02750"
                     /inference="COORDINATES: protein motif:HMM:NF017807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNS27020.1"
                     /translation="MRTRDVVDMLLLGGIWGGAFVLLRVASPEFGAVPLAAVRILVAA
                     FTLLLFLRDRSLLRAEPLRLLLLGLIASAIPFTLYAYAMLSITSGLAALLNATTPMFG
                     ALVAWLWLGERLSVARVVGIVIAFAGVLWLVNDALGTLGVGALGGVLAALLGAACYAV
                     AASYTRRVFPTADPVALATGTVLGAFVAILPFAVVTWPAAPISLRAWMAAVALGVVCT
                     AIAYKVYFRLLRNVGAGRAVTVTFLFPPFGVLWGALVLAEPVTLSLLAGCAVVLAGTA
                     LAVGLFDRVRY"
     gene            10540..11646
                     /locus_tag="PKN91_02755"
     CDS             10540..11646
                     /locus_tag="PKN91_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNS27021.1"
                     /translation="MSQIRNLITAASVIVALLAGGWAIYATRHAGDRVAPVDLAAGPR
                     SDSAVQGAAVAVSVVTGEVRSERITRELQALGTAVANESVSITSKATNLVMAINFQDG
                     QAVKRGQVLVKLDDAQARADLAEASAALTESTSQYERARDLLSTRVLSQSQYEQLEAT
                     MKANRARVDAAQARLDDTVIRAPFDGRVGLRRVSVGSLINPGTVITTLDDTSVMKVDF
                     AVPENQIGSLRSGLALTARTAAHPDRLLEGRIMTIDSRIDPTTRSVTVRALVPNDSGL
                     LRPGMYVNVSLAREEYEALVIPEQALVPEQSRQYVFVVEDDRVARREVQIGRRTPGRV
                     EVVSGLAAGERIIVEGTQKVREGSLVAEAVNQPV"
     gene            11745..13265
                     /locus_tag="PKN91_02760"
     CDS             11745..13265
                     /locus_tag="PKN91_02760"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HNS27022.1"
                     /translation="MSATPYQQLEQEWKRLHAFRGALALLRWDAAVMMPKGSAENRGE
                     QLAALETEYHALLTSPRISRLLDRAQANVQGYTDWQVANLREMRRQRDHAIATPVSLV
                     SRLARATSRAEVQWVEARRADDFAIFAPHLEEVLHLVRDKAQLLGQALNLAPYDALID
                     EFSPGITMVDIDAMFKALSRRLPTLIREAMALQEKHPPAPLTGKFPVAKQRQLVNELM
                     KALGFSFETGRIDESEHPFTEGVPGDIRISTHYSTDDLFSGVLGALHETGHAIYAHGL
                     PSEWRDQPVGRDRGMAIEESQSLLVEMILCRSRPFVRYLQPLLVKHFGVEGPEWEVEN
                     IYAHLTRVRRGLIRMDADELTYPLHIQLRYELEKKLLSGQLAVRELPLAWADFMSGRF
                     GVTPANHREGCLQDIHWAVGSFGYFPSYALGAAIAAQLYEKMRIDIGALDEQIARGEF
                     GALIGWLREQVHSLGSRLTIQELLKHATGKPLSAASALRYLEGKYLEDPASRTAAA"
     gene            13171..14070
                     /gene="ttcA"
                     /locus_tag="PKN91_02765"
     CDS             13171..14070
                     /gene="ttcA"
                     /locus_tag="PKN91_02765"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HNS27023.1"
                     /translation="MRPASRCRRRPHCVTSKASTSRIRPPAPPPPDVTAPQPVDSNTS
                     KRLAAHLRGLAGKAIEDFRMIEAGDRVMVCLSGGKDSYTLLDLLLSLQRSAPVDFEVV
                     AVNLDQKQPDFPAHVLPDYCRALGVPFHQIEQDTYSVVKRVIPEGRTMCGLCSRLRRG
                     ALYRFAEEHGFTKIALGHHRDDIVETLFLNLFHGGRLKAMAPKLVSEDGRNVVIRPLA
                     YVPEREIERYARARAFPVIPCRLCGSQDNNQRVAVKRMLAAWEREHPGRVASIFSALT
                     QVEPAHLADPALFDFASITRAPQ"
     gene            14067..15533
                     /locus_tag="PKN91_02770"
     CDS             14067..15533
                     /locus_tag="PKN91_02770"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNS27024.1"
                     /translation="MIELPFSNAYWVREGRWLAGEYPGGPTREDTRARLAALASLGID
                     TWLDLTSPGELPAYEGELTRGLLHIRKPIPDHGVPLEPEHMSDIIEVIDGLLADGRNL
                     YLHCRAGIGRTGMVVGCYLAQHGRRGDAALDALNALWSGSARARSWPSIPETDEQRNF
                     VYRWPAILDPTLDDEVMAATHALRERFSGALLGLAVGDALAAATQYRKPGSFTPVGDL
                     LGGGPFDLPRGAWSDDTAMALCVADSFLERGGFDPVDQVERFMRWQRDGYLSATGQCV
                     GITAGVTRALAAARWRRQRFPGSHDPALLDPEPLTRMAPALMFHFADPAVAIDTAVEL
                     ARTTCQAPLVLEACRVLATALHAALSGRDKAVILHGVRLLGRGGGLRPEVAQIAAGGY
                     RNALAAPAPAQATALDVLGATLGAFETTSTLREALLLCANRGRDADVLTAACGQLAGA
                     HYGAAAIPAAWRNSLIGKEIVSDFADRLLARALEGLAG"
     gene            15631..16719
                     /locus_tag="PKN91_02775"
     CDS             15631..16719
                     /locus_tag="PKN91_02775"
                     /inference="COORDINATES: protein motif:HMM:NF027219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HNS27025.1"
                     /translation="MPILVHRRPAARARARSPRKAAPARRNLKWARLRDEDLLQMRFC
                     DLKLKLDRTPLARHMRTLYRDLERRGIDFRPHAWFGEEWFSPDGVPGISIPFYLAHPR
                     LERLERTMMKNVEGGNAQWLKRILRHEAGHAIDTAYRLRRRKRWRETFGPASLPYPDR
                     YRPRPGSRRYVQHLGDWYAQSHPTEDFAETFAVWLTPNSSWRRGYADWPAYQKLEFVD
                     ELMAGLRGKRAPVRSRARIEPIDTSTRTLAEHYSEKLERYSEDRRGLIDVMLSKVFMP
                     SRPRRTTPRASTLMRAARRELVDAVVREQGVARYSAFQILRMIIERSDQLGLYPRGSR
                     RDALRNARWMLSRLVSLYAESTSPQMRL"
     gene            16716..17750
                     /locus_tag="PKN91_02780"
     CDS             16716..17750
                     /locus_tag="PKN91_02780"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27026.1"
                     /translation="MKKLKVLVVVHATLVPPDSLNGRSEKEIEEWRTEFDVISTLKKR
                     GHEVRCLGVLDTLSELRGAITDWKPDIVFNLLEEFDGIVTYDQHVVAFLELMRQPYTG
                     SNPRGLLLSRDKSICKQLFAYHRLPSPQFAVFRKGVKFKLPRKLKFPLFVKSTTEDAS
                     LGIAQASVVEDEAKLRERVGFVHEQIGSNALVEEYIEGQELYVGVLGNDRLTRLPVWE
                     MVFGSMPDSLSAIATRKVKWDRRYQKKYGITTRAADELPREIVSRLDSLSRRIYRALG
                     LSGYARMDFRLAPDGRIFVLEANANPNLAAEEDFALSALEAGLEYPELLERIIALGLA
                     YRAEWRVFYG"
     gene            17743..18345
                     /locus_tag="PKN91_02785"
     CDS             17743..18345
                     /locus_tag="PKN91_02785"
                     /inference="COORDINATES: protein motif:HMM:NF017966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0017009 - protein-phycocyanobilin linkage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpcT/CpeT family chromophore lyase"
                     /protein_id="HNS27027.1"
                     /translation="MVERGLALIMLALLGACSSTADLRKAELSELAAWLPGTYVNGAR
                     ELAFVPIYAPFISDNVFFTEESSTQGGRRVLSQRVIAFEVVDKQIVQASYAFAEPGRW
                     RAGLYNPDLFKGLMEQDLKVQAGCEMLWVKDVDAGKFAGANDRKQCRASRASGALAFL
                     EVRAELTPDDFARAERYYNAGGRLLSGSEDDELERWRKSE"
     gene            18342..18665
                     /locus_tag="PKN91_02790"
     CDS             18342..18665
                     /locus_tag="PKN91_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27028.1"
                     /translation="MKLFISRAIRKLDDTTQKMRALRAPRPAPPEKPRAAAPGEAGSG
                     RVRHDERGNAVWDIAVATGVFALESTSRLLKRLEAPELRIEDDKELKLEDDKGGGYDP
                     YNRRR"
     gene            complement(18678..20690)
                     /locus_tag="PKN91_02795"
     CDS             complement(18678..20690)
                     /locus_tag="PKN91_02795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNS27029.1"
                     /translation="MLKPAALTLCLTGLAATAALASAPFTADDLVSLHRISDPQVSPD
                     GRHVAYVMRETDREANRGRTDLWLLDLSARNPAPRKLTHHAAGDTHPRWAPDGSALYF
                     LSTRSGSAQIWRLALGGGEPLAVTDLPVDVGSFRIAPTGDRLVFSMAVKPPEEARKGS
                     SKASGLVYDKLFMRHWDTWSDGSRSHLFSAALDASGRIGTPIDLSQGLDGDVPSKPFG
                     GDEEYAISPDGERVVFSLRIAGRTEPWSTNFDLFEVAIDGGTPVNLTADNPAWDTSPV
                     FLANGDLAWLAMQRPGFEADRFHVMLRRAGREARALTRDWDRSVARLGATRDGKRLLA
                     TADEIGQRALFAIDPVSGKPSKLVGSGQVTEFAAANGGIVFSWANLAAPADLWWLRDG
                     GKPERLTDANAARLGGRALAEFEQYSFRGWNDETVYGYVMKPHGYMPGNKYPIAFIVH
                     GGPQSSFANAWSYRWNPQVYAGRGYAVVFIDFHGSPGYGQAFTDSISGNWGGAPFVDL
                     QKGLAAAIERYDWLDADKACSLGASYGGYMQNWIAGNWPERFRCIVNHDGIFDNKSMY
                     YTTEELWFPEWENGGPYYEQPANHDKWDPSRLVGEWRTPMLVIHGANDYRVPDAQGIA
                     TFTALQRRGIASKFLYFPDENHWVLKPANSLQWHAEVLDWLDTWLK"
     gene            complement(20738..21403)
                     /locus_tag="PKN91_02800"
     CDS             complement(20738..21403)
                     /locus_tag="PKN91_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HNS27030.1"
                     /translation="MNLTLLIGNQNYSSWSLRPWMLLRNLGLEFATDKVQLDSPQFAA
                     QVAAFSPAGRVPVLRDGELVIWDSLAICEYACELAGRGWPRERAARARARALAAEMHS
                     GFTELRSAWGMNSRARNRRTAMTPGLAADIARIDAAWSECRREHAAQGPWLFGADYTV
                     ADAMFAPVVLRFNTYAARTDGVALSAAAAAYVTHVLLDPILGEWMSAAEAEPWVLPHA
                     EIG"
     gene            complement(21400..22668)
                     /locus_tag="PKN91_02805"
     CDS             complement(21400..22668)
                     /locus_tag="PKN91_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS27031.1"
                     /translation="MTRYQWLVLAAAWLGWGFDVFDALLFNFVAPNTVPTLLGLDPGG
                     SEARDATVLWTGILSSILLVGWAAGGILFGWYADRYGRRRALFVTVSLYAIGTALCAA
                     SMNMGQLVVFRAIASLGIGGEWAVGAALVAETLPENRRVFGGTLLYTASPIGLLLAGL
                     VNYQVAGVWFADDPANSWRYVFLCGLAPVALAFAVRAFIHDPEAWAHARAHSAPPSPR
                     ELFTPEVRRATLVGTLVSVAGLLTWWACNAFVPLLGAWLAVENAPGEIERWKTIASNA
                     FNLGGLVGALAALPLAYWLGRRAMFAAYFVYSAFAIVATFGLEFAPELRIRLLFFVGL
                     GVYGIFSTYVFYLPELFPARLRASGAGFCYNIGRVVAAAGPLAIGIVTSAADGSSAAL
                     LGTMIWVAVIPFAAAMLTPLVIETRGMKLQ"
     gene            22796..23401
                     /locus_tag="PKN91_02810"
     CDS             22796..23401
                     /locus_tag="PKN91_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27032.1"
                     /translation="MVVTVHSLREYFREALRETLARQHVEVGGETEQYVVNMLTLFAR
                     SEALFEQTPEGPRIKPLVVMLGEALEAGSARERCAGLQRLGDVSLFVAGFMAQGFARK
                     LVDIDYHIAMGGRAYGTLSDAVRQSGRPALGPVFGELAAKFQLLVDALNEVSESGYQH
                     SDRDILRLYELWIKTGSRRAERLLAKLGVSPAAGARSSFQH"
     gene            23413..24063
                     /locus_tag="PKN91_02815"
     CDS             23413..24063
                     /locus_tag="PKN91_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27033.1"
                     /translation="MVLRALQDLLAAIYDVPLAHDVYDFLVTDPGCVPGAERSAANEE
                     QLLVARDGDDMAVALFLDAQLLERLAAADPLERLDGGNLADCWTALEGVSHFHYLAFH
                     AGHDREVSRMELETQAEVDKYVATLWLLRSQSPERFPRELHRILFSCTQVDPRLPPQR
                     AQLYRRASAYAARFCRRMEHRLKHPADAAFRDMVAELRRFYRLTEARKLAHIERCA"
     gene            complement(24067..24384)
                     /locus_tag="PKN91_02820"
     CDS             complement(24067..24384)
                     /locus_tag="PKN91_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27034.1"
                     /translation="MAGDSDKPPSGARRVAQVVHDDLGNARVEWRKVPDDRTGNFARP
                     TFTIEGEGPDMGADPYDRTVPSGRRDSHGTGTTRRDLRKLSEWIKLKREMEERKQRGE
                     DDE"
     gene            complement(24456..24986)
                     /locus_tag="PKN91_02825"
     CDS             complement(24456..24986)
                     /locus_tag="PKN91_02825"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNS27035.1"
                     /translation="MTASTEVVELPNGHRVTLDIVHHPGGAAIVAVDAALRVCVLRQY
                     RHAAGGWIWELPAGKLEPGELPLATAQREIVEEAGILATRWDSLGTILSSPGVLTERV
                     HLFLATALEPAAQATESGELLEVHWLPIDDACRRVLAGEFADAKTCVGLLRAAGHPAL
                     TALRTGRDVNCGPSGM"
     gene            complement(25060..25479)
                     /locus_tag="PKN91_02830"
     CDS             complement(25060..25479)
                     /locus_tag="PKN91_02830"
                     /inference="COORDINATES: protein motif:HMM:NF039479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6524 family protein"
                     /protein_id="HNS27036.1"
                     /translation="MAQESIGIGGVAIRFALALALVLLSYNPGGVSYYHWVAGDFPNF
                     DALQGLAGVVLLIGWVVFLTATARSLGAIGVLLSVAFFAALVWVAVQYGWFEAAPRTL
                     LWIGLAVAAAVLAIGMSWSHIRRRLTGQLDVDEVDTR"
     gene            25545..27629
                     /locus_tag="PKN91_02835"
     CDS             25545..27629
                     /locus_tag="PKN91_02835"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNS27037.1"
                     /translation="MSTTAHLAKLNVAQRKAVTYGEPAESQGYRSGPLLIIAGAGTGK
                     TSTLAHRVAHLAMHGVDPARILMLTFTRRAALEMRRRAHEVAKAALNDVIGGVSQTIL
                     QRLGWAGTFHSVGNRLLRHYARHLGLDPQFSVIDRADSADLIDGIRQELGFASKEQRF
                     PRKDTCLAIYSYRVNTQLSLEDSLERQFPWCGQWADELTKLFRTYVERKQKFAILDYD
                     DLLLYWHILMSEPKLAQHVATHFDHVLVDEYQDTNRLQAEILYGLCPDGNGLTVVGDD
                     AQAIYSFRAATVENILEFPEHFQPRAEIVALAQNYRSTQAVLDVSNALMAEAPRQFRK
                     HLLSLRGSGSRPRLVTVEDLPTQAEYVCTQVLKAREANVPLRRQAVLFRSSSHSDLLE
                     VQLSKRKIPFIKYGGLKFLEAAHVKDLLGILRWIDNPRNAMAGFRVLQLLKGMGPANA
                     RRALDHLLQQGGSFESLRSFEPPADSAVDWRKLVDLLVTLADPQQPWTGQVNQVRNWY
                     RPHLERLYEHVHTRIGDLDQLELLAGQYPSRERFITELTLDPPQASGDLAGQPLLDED
                     YLVLSTVHSAKGMEWDTVYVLNVVDGSFPSEFATGRAETIEEERRLLYVAMTRAKDDL
                     MLVQPLKFPLTHQPKQGDAHVYGGRSRFLTEKVLKTLEATTFQGSNLGEPSLGAAGQT
                     TLDAVERVKSMW"
     gene            complement(27658..28461)
                     /locus_tag="PKN91_02840"
     CDS             complement(27658..28461)
                     /locus_tag="PKN91_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNS27038.1"
                     /translation="MAGVLALAACATTTSTRSSTAAALDRILAGDHRSAENRARDVYR
                     HPRETLLFFGIRPEQTVVEIWPGGGWYTEVIAPLVEGKGKYYAAHTPPNPGSAGVTRS
                     LKAYADKLAARPELYGSATVTALGPGVGEIAPPGSADLVVSFRNIHNWMAAGFAPEAF
                     AAMYKALKPGGVLGIVEHRGNPAVPQDPKAANGYVNEDYAIRMIEAAGFRLVAKSEVN
                     ANPKDTKDYAKGVWTLPPRFAAGDENREKYAAIGESDRFTLKFIKPSGN"
     gene            complement(28579..28860)
                     /locus_tag="PKN91_02845"
     CDS             complement(28579..28860)
                     /locus_tag="PKN91_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNS27039.1"
                     /translation="MTTIYVGNLPFTATEQDVRVLFEAHGTVESVKLINDRETGRPRG
                     FGFVEMPEGDAREAIQAMNGREMNGRTLRVNEAQERPQRPRSNGGPYRR"
     gene            complement(28955..29857)
                     /locus_tag="PKN91_02850"
     CDS             complement(28955..29857)
                     /locus_tag="PKN91_02850"
                     /inference="COORDINATES: protein motif:HMM:NF020468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1838 family protein"
                     /protein_id="HNS27040.1"
                     /translation="MRHAATVPTRLLLAAGLLAVTASAHAGSIDLATPEGANLAMRKV
                     QCSLKDNEPVTYYWNGDVYSRVEGEPDRKLFHFEAMNIRTCVTVNDAEKGAGWRLVSR
                     ELLLYMDPKTGEVLDTWQNPWTGKSVKVLHIDNDPVNQPPMFPVGRDGKPVTWPARSL
                     GDNWWLTLTIPLFYNNPLGGPYQPYVGGMYHATEMFNFMGDISDLVDDARDTAAVRVG
                     WVRQSGWLPWMEMGGRPGLVYFHAAGRKIERFADLPHLLQHEIETNYPKYKSPPPGDD
                     TRPNETSWTYFMKKVPPPAPPARH"
     gene            complement(29999..>30598)
                     /locus_tag="PKN91_02855"
     CDS             complement(29999..>30598)
                     /locus_tag="PKN91_02855"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184848.1"
                     /note="catalyzes the formation of agmatine from arginine
                     in putrescine and spermidine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HNS27041.1"
                     /translation="CRRLEETPEELEGLEAILSDIYFCNMSVFQSLPDSWAIDQLFPI
                     MPIHRLDERPVRKGVLADITCDSDGKIDRFVSPRDVKRTLELHNVANGDEYYLAAFLV
                     GAYQETLGDLHNLFGDTHVVHIRQHDEGGWTIDEVVKGDTANKVLEYMEYDVEELYPA
                     LSRDCERAIRAGRMTIPESQALKRFYERELNGYAYLEPA"
BASE COUNT         4798 a        10379 c        10529 g         4882 t
ORIGIN      
        1 tggcggcgaa cgggcagtac gcgatcgaag tgcgggtgcg tccggcgcac ccgctgctca
       61 cgcatcctta ctggatgggg ctcatgaagc gtctcgcctg agcaccacgc cgccgagcgg
      121 cggcagcgtg atctcgagcg agcagggctg gccgcgatgc ggcgtcggtg tggcggggag
      181 cggcagcgga ttgccgaggt tgctgccgcc gtagtactgc gagtcggaat tcaggatctc
      241 gcgatagctg cccgcctcgg gtacgccgat gcgcagcgcg atgcgcggca cgggcgtgaa
      301 gttcagcgcg acgaccacga agtgctcgtc gctgcgtcgc acgtacacca gtatcgagct
      361 ggcggcgtcg tcgcaatcca gccactggaa accccgcggt tcgaagtcgt aacggtgcag
      421 actcgggtcg ccgacataca gccggttcag gtcacggacc agctggcgca ccccggcgtg
      481 cgccggatcg tcggcgagcg accacggcag cgcggcggca aagttccact ccgagtcctg
      541 ggcgaactcg ccgcccatga acagcagctt cttgccgggc agcgtccaca tgtacaggta
      601 cagcaggcgc aggttcgcga gctgctgcca gcggtccccg ggcatgcgcc cgagcagcga
      661 gcgcttcaga tgcacgactt catcgtgcga cagcggcagc acgaagttct cggtgtacgc
      721 gtacatcatc gcgaacgtca gccgctggtg gtggtagctg cgaaacagcg gatcctggcg
      781 gaaataggcg agggtgtcgt gcatccagcc catgttccac ttcatcgaga agccgaggcc
      841 gccgtcgtgc gccgggcggc tgaccatggg ccagtcggtc gactcctcgg cgatcaccac
      901 ggcgcccggg cagcgcgcgt gcaccagcgc gttcagctcc ctcaggaagt cgatcgcctc
      961 gaggttgtgg ttgccgccgt agcggttcgg cagaaaatcg tccttgcggc tgaagtcgag
     1021 gtagagcatc gaggcgaccg cgtctacgcg cagaccgtcg aagtggtact cctcgagcca
     1081 ccagcacgcg ctcgagagca ggaacgagcg cacttcgttg cggccgtagt cgaagaccag
     1141 cgtgccccag tcgcgatgct cgccgcggcg cgggtcggcg tattcgtaga gcggcgcgcc
     1201 gtcgaaacgc gcgagtccgt gcgcatcgcg cggaaagtga cccggcaccc agtcgaggat
     1261 cacgcccagg ccggcggcat ggcagcggtc gatgaagtgc cgcaggtcgt ccggctcgcc
     1321 gtggcggctt gtcggcgcga agaacccggt cgtctggtag ccccaggaat cgtcgagcgg
     1381 atgctcggtg accggcagca gttcgagatg cgtgtacccg aggtcgcgca cgtacggcac
     1441 cagcgtgtcg gcgagttcgc ggtagcccgg gaagcccccg tccgcgtggc gctgccagga
     1501 accgaggtgc acttcgtaga tgctcatcgg cgcgtgcagc cagtcgcgcg cggcacgcgc
     1561 gcgcagccag gcctcgtcct gccaggcata tgacgttgcg gccggcaggc gcgaggcggt
     1621 ggcgggtcgc agttcagcgc ggcgcgcgta ggggtcggac ttcaggctga gcgtgccgct
     1681 gtcgcggttg cggatctcga acttgtagag ctcgccgggc gcgaggtccg gcacgaacag
     1741 ttcgaaaacg ccgctcgatc cgcgcgccga cagcgggtac tggcggccgt cccagttgca
     1801 gaacggcccg acgacgctca cgcgctcggc gttcggcgcc cagaccgcga agcgcatgcc
     1861 tgcgacgccg tcgcgcacgc gcggcaccgc gccgagcagc tcccatgccg cagcgtgcgc
     1921 gccgcggccg aagcgctcga gcgccgccgg ttcgagctcg ggcgcgaacg cgtaagggtc
     1981 gtggcgcgag tgggccccgt gggcgtcgcg ccagcgcagc cgatagcgct ccggtacgtg
     2041 ggctgcatcg ccctgccaca ggaagaaatc ggtctggccg acccgcggca cggtgagcgt
     2101 gtcctcgagc atcacgtcgt gcgcgcgcgg caggtacagc aggacggcga gccgggtccc
     2161 ttcgcggtgc gggccgagca ctccatgcgg atgatggtgg cgcccgtccg ccaggcgccc
     2221 gaggtcgtgc gcgagcgtgt cgagcaatcc gtcggggttc gacatttggc gtgtgatcgg
     2281 gttgcgcctg aaacacaaag gtgtaaacgt acgtctacca tatgaccaag caagccgcac
     2341 gcgcatcatc ggggcgttac gtcagccgac tcaccagcgg cgcgcttgcg gtcatcatgg
     2401 cgggcgggcg cggcgagcgc ctgggaccgc tgaccgagag ccgctgcaag cctgcggcgc
     2461 cgttcggcgg caagttcctc atcatcgatt tcgtgctgtc caactgcgtg aactccggca
     2521 tccgccagat catggtgctc acccagtaca aggcgcagtc gctgattcac catgtgcaga
     2581 acggctggaa ctacctgcgc ggcgaattcg gcgagttcgt cgacgtgatc ccggcgcagc
     2641 agcagatcgg catgcactgg tatcgcggta ccgccgattc ggtctaccag aatctcgacg
     2701 ccatcctgca actgcggccc aagtacgtgc tggtgctcgc cggggaccac atctacaaga
     2761 tggactacgg cccgatgatc gcctaccacg tcgagaaggg agccgacatc accgtcgggg
     2821 tggtcgaagt gccgcgcgcg agcgccgcga atgccttcgg cgtgctgacc gtgaccgaat
     2881 cgaaccgggt cacgcgcttc accgagaaac ccgccgatcc ggatccggtg cccggcaagc
     2941 ccgacagcgc gctcgcctcg atgggtatct acgtgttcag cacgcgtctg ctcgagcaac
     3001 tgctggtcgc cgatgcggcc gatcccgatt cgaagcacga tttcggccgc aacatcattc
     3061 ccggcgcgat tccggacatg caggtgttcg cctatccgtt ccaggacgtc aagacgcgcg
     3121 cccagaacta ctggcgcgac gtcggcacga tcgatgcgta ctacgaggcc aatctcgaac
     3181 tcgtgcatgt cgcgcccgaa ctcaacatct acgacgagga atggccgatc tggacctacc
     3241 aggtgcagca gccgccggcc aagttcgtgc tgaacgacgg cgcgcgcgtc ggcaccgcga
     3301 tcaactcgat gatcgcgggt ggttcgatcg tctcgggtgc acgcgtcacc gagtcgctgc
     3361 tgttctcgaa cgtgcgcgtc aacgagtaca gcacggtgca tcgctcgttg gtgctgccgg
     3421 gcgtgaacat cggcaaggac tgccacatca acaacgccat cctcgacgag ggctgcgatg
     3481 tgccggacgg tgcgcgcatc ggtatcgata ccgcggccga tcgcgagcgc tatttcgtga
     3541 ccgagaaagg cgtcgtgctc gtgaccgccg cgatgctccg cgcggccggc gcctagttcc
     3601 atgtcgggtc gtctgccggt cgtgctgctg tggcacatgc accagccgca gtactgcgat
     3661 gcgctcaccg gcgagtacac gcagccgtgg acagcgctgc atgcgctcaa ggactactcc
     3721 gacatggcgg cgcacctcga ggacaacccg gctgcgcgtg cggtggtgaa tttcacgccg
     3781 gtgctgatcg agcagctcga ggcactggcg ctgcaggtga gggcgcacct cggcatcggc
     3841 gccgcgctgc tcgacccggn nnnnnnnnng cccgaggcgc gcctggcgct ggtgcgcgcg
     3901 ctgctgcgcg cgcagcgcgc gcagatgatc gagccgctgc cggcgtatcg tgagctcgtc
     3961 gacatcgccg cgctgctcgc gactccggcg cgcatcgcct acgtgagcga cgcgttcatc
     4021 cgcgacctct cggtctggta tcacctcgcg tggaccggcg agacgatccg tcgccgcgac
     4081 gcgcgcgtgg cggcgctgat ggcacgcgcg cgcgatttca cgacggacga tcgccgcgcc
     4141 ctgctcgccg tgatcggcga cacgctcgcc tcgatcattc cgcgctaccg ggcgctcgcg
     4201 gcagcgggac gctgcgaact gagcgtgtcg ccgtattccc acccgatcct gccgctgctg
     4261 tgcgactttg ccgccgcgcg cgagtctctg ccggcgagcc cgctgccgag gcacgccggc
     4321 tacgcgggtg ggcgcgaacg cgccgactgg cacatggggg aagccgtgcg cgtgttcgag
     4381 cgcgccttcg gcgcccggcc ggtgggctgc tggccgtcgg agggcgctat cagccacgcg
     4441 gcgctcgagg tgatcgatcg gcacgggttc gcctgggtcg cgagcagcgc aggcgtgctg
     4501 cgcgcgagcc tcgatgcacg gtcgggcgag gtagcctggg cggccgatgc cctcgatcgc
     4561 ccgtatcggc tgccgggaca gcggctgcaa tgctggttcc gcgacgacgg gctgtccgat
     4621 ctcgtcgggt tcacctattc gacctggcac ggggacgacg cggtcgcgca cctggtcgcg
     4681 gcactggtcg agctggcggc gcgccatggc gactcgccgg ggcacgcggt gctcatcgcc
     4741 ctcgatggcg agaatgcctg ggagcactac ccgcagaacg gctaccactt cctgcgcgcg
     4801 ctctatgccg cactcgccac ccacccgcaa ctcgagctca cgacactcgg ggcgctgtcg
     4861 gcgcgcgagt cgctgccgcc ggcgctcctg ccgcacgtgc gcgccggcag ctgggtgcat
     4921 tcgacgctcg ccacctggat cggcgatgcc gacaagaacg cggcctggga cctgctgtgc
     4981 gaggcgaagc tggcattcga ccgcgtggtc gcgggcggcg cactggtcgg cgagtcgctg
     5041 gccgcggcgc gacgccagct ggcgcagtgc gagagttcgg actggttctg gtggttcggc
     5101 gattacaacc ccgccgaagc cgtggcacag ttcgatctgc tgtttcgccg ccagctgacc
     5161 gtcctctatc gcatgctggc gctcgaacca cccgcggaac tcgggcagcc ggtctcgcgc
     5221 ggcggcggct cgatggaaca cggcggcgtc atgcgccgcg cccatgacga ctgaatcgcc
     5281 acgtcgggcg ggcgtgctgc tgcccatgtt cgcgctcccg cgcgcagcgg ccggcgccgt
     5341 cgcggcgcgc gcgttcgtcg actggctggc cgcagcgggc ttcaccgtgt ggcaggtgct
     5401 gccgatcggc cccaccggtg ccgacggctc gccctactgg ctgcgctcgg atcatgcgac
     5461 acacatcgcg ctgatcgatc ggcaggacta ctcggccgac gactaccacg catggcgcga
     5521 gcgcgaacgc gactggatcg acgactacgc gctgttcgag gtgctcgccg ccgcgcacgg
     5581 cggcgcgccg tggcaggagt ggccggtggc gctgcgcgat cgcgagcccg cggcgctcgc
     5641 tgcggcgcgc gcctcgcacg ccgaggccat cgggcgcatc gtcctcgacc agtaccttgc
     5701 cgctcgcggc tggagcgagt tgcgcgagta tgcgaattcg cggggcgtgg cactcttcgg
     5761 cgacctgccg gtctacgtgg cgcccgattc ggtcgagacc tgggtgcatc ggcgccagtt
     5821 ccagctcgct gcggccggcc gaccaacggc actcgcgggt gtcccgcccg actacttcgc
     5881 tgcggacggg cagctgtggg gcaatccggt ctacgactgg gacgcaatgg ctgccgacgg
     5941 atacgcattc tggtgtgcca gggtgcggcg agcgctgtcg cgctacgacc tgctgcgcct
     6001 cgatcacttc cgtggactgg tcgcgtactg gcgcgtgccg gccggtgcag ccacggcgcg
     6061 cgacggcagc tggcagccgg ccggcggcag gccgctgctg catgcgctgc aggcgctcgc
     6121 gcaggatcgg ccgcatcccg ggtcttcgac ctacgtcgcc gaggacctcg gcgtgatcac
     6181 gccggatgtc gaagcgctgc gctgcgagtt ccggttgccg ggcatgaagg tgctgcagtt
     6241 cgcgttcgat ggctcggccg ccaatcccta cctgccgcac cgccatatgc gcgagtcggt
     6301 cgtgtacacc ggcacgcatg acaacgacac cacgctcggc tggtaccagg gcctcgacgg
     6361 gcacacgcgc gcgcgcgtcg actacgtgct gcgtgtgcac gccggggaga tgccctgggc
     6421 gctggtccac gcggcactcg cctcggtcgc cgacctcgcg atgctgccgg tcgccgatgt
     6481 gctgggcctc ggcggcgcgg cgcgcctcaa tacgcccggc acgacagtcg gcaactggca
     6541 atggcggctg ccgcccggtg cattgacgcc acagctggcg gcgcagtttc gccacgccaa
     6601 ccaggtattc ggccgcgctc tgctaggata ggcggcatga ggattctcat cgccaccgcg
     6661 cttgcggtat tgctttcctc ctgcgcggcc acccgcctcg aaacacccca cctgcagctc
     6721 gtcaatatcg agatgatgga aagcgagctg ttcgaacagc gcttcaaggt gcgcatgcgc
     6781 gtgcagaatc ccaacgaccg cgcactgccg gtgcagggca tcagcgcgaa gatgcagctt
     6841 cagggcgagg attttgcgca gggcgtcgcc ggcgaggcgt tcacggtgcc ggcgttcggc
     6901 gaggccgagt tcgacatggt cctgcgcgcc aacgtggccg gcgcgatcct gcgactcctg
     6961 tcttcgatga aggataagaa gagtccggac aagctcgact accgggtggt cggcaagctg
     7021 tcactgtcct cggggttcct gcgctcggtc ccgttcgagg aatcgggctc gttcaacctg
     7081 ggcgaactga cgaagggctc gggccggcct tgagcgcgcc gggcgcggct cagcgcccga
     7141 gcacccgctc atacagcgcc acataccgcg cactctggcg cgtccacgag aagtcctgtg
     7201 ccatgccgtt ctgcatgagc cgggtccacg ccggctcgtc ggcgtgcagc gtgagcgcct
     7261 gctgcagcgc ccaggccatg cccgccgaat cgaaatcgtt gaacacgatg ccggtaccgc
     7321 gcccggtcgc ggcatcgaag tgctcgaccg aatcggcgag cccgccggta cgccgcacga
     7381 tcggcacggt gccgtagcgc aggctgtaca tctggttgag gccgcagggc tcgtagcgcg
     7441 agggcatcag gaacatgtcg ctcgccgcct cgatccagtg cgcgcgcgcc tcgtcatagc
     7501 cggcgtgaaa gcgcacctcc tgcgggaaat cgcgtgccag gcgcgcgaag aactgctcgt
     7561 agcgcgcctc gccactgccg agcgcgacca gttgcaggcg ccgcgccgcc agcagcggcg
     7621 gcaggctgtc gaacagcagc tcgataccct tctgttcggc cagccggctg acgactccgg
     7681 cgagcggtac gcccagcctg aacggcagac cctcgcgcgc gaggaaggtg cgcttcaact
     7741 cggccttcgc gccgaggcgc tgcgcgtcat agtggatcgg cagccagcga tcgtggcgcg
     7801 gatcccattc gtcgtaatcg acgccgttca ggatgccggt gagcgagtcg ccgcgcgcgc
     7861 gcagcgtctc ctgcaacccc atgccgtatt cttcggtgca gatctcgcgc gcgtaagtcg
     7921 ggctcacggt cgtcacggca tgcgcataaa ggatgccgtg cttcagcgca ttgacatggc
     7981 cggcacgcag gtcttcctgg tgcaggcgcg agaggtcgtc caagccgatg tcgcccgcgt
     8041 actccgcatc gaagctgccc tggtagccga tgttgtggat cgtcaacacg ctgcgggccg
     8101 cggcaaacag cgggtccgcg ccgtactcgg cggcgagcag cagtgggccg aaggccgtgt
     8161 gccagtcgtt gcagtggagg atgtcgggcg cgaagccgag gcggcgacac agttcgaacg
     8221 cggcgcgcgt cagcctgatg aagcgcaggt gctcgtcggg atcggcggtg tacagcgtgg
     8281 cgcggtcata cagccgcggc tcgtcgatga agtacacggt cgccggggat ccgggcagcc
     8341 tggcggtcgt gatcccgccg ctggtggaga ggccgtgcgc ggcgcgatcg atgctcgagt
     8401 agagaggcgt gacagtgacc acctcatggc cggcggcggt caggtgcttg gcgagcgccg
     8461 cggagacgtc cgcgagcccg ccggttttcg agaacggggc gacctccgaa gcgagcaggg
     8521 cgatgcgtaa cggcacgccg ggcatcttac cgaacgcgcg aaacgccgcc gtcccaatta
     8581 tgatggatga cttatgttgc gacgtctgtc actcgtgctc gtcacctcgg gcgccctgct
     8641 cgtgtctgcg ggcatcggcg tgctcgcggc cgattggccg ttcctgcacc ggctgtggca
     8701 gctgcccgcc gatgtgtcgg gctggcgcga gaccgtcgcc gtaccgaccg tcacgatcgc
     8761 cggcagcgat gcgccgtata ttccgccggc ggcgccggat ccactcggca tcgatgccgc
     8821 gctgctcgag caggcggcga gccaggccga ggccgaggac agcgtcgcgc tgctcgtcct
     8881 gcaccgtggt gcgctgctga tcgagcgcta ctggcacggt atcacgcgcg atacgctgta
     8941 tccgctcgag ggcctgaccc gtgcgctgct cggcatcgcc tatggacgcg cgctcgatga
     9001 cggcagcatc gaatcgctcg acgcaccgat cgagcaatgg ctgcccgaat ggtacggcga
     9061 gccgcgcggc gccatcacga tccgccagct gctctggaac gtgtcgggtc tcgaaacacc
     9121 agcggaggaa ggctggtggg gacgcctcgg caaggccggc aggctgcgct tcggcaccac
     9181 cttcgagcgc accgcgctgt catatcgcgc cgcgcacgag ccgggcaccc gcttcgaact
     9241 gtcgcgcgtc gatgcacagc ttgccgcgct ggtcctcgag cgggcgagtg acgaggatta
     9301 tgccggcttc atcgaacgat cggtgtggca acccgcgcgt gcggggcgcg ccgagttcac
     9361 gctcgatcga ccgcacggcg cggctgcggc attcgacggc ctgcgcgcca ccccgatcga
     9421 cgtgctgaag atcggcgcgc tgctcgcgct cgatagcggc tgggcgggcg agatggcgcg
     9481 cggttcactg ccgaacccgc gctacggcct gcaggcgtcc gccgtcgacg gcgacggcct
     9541 cctgctttcg ggcgacggcc gcgccgtgtg ggtctggccc ggggagcagc tcgtgatcgt
     9601 ccggctgggc gatgccgcgc cgggctggga tgcgagcgca tgggcgcagg cgctgcggcg
     9661 tagtctgcac tgatgcgtac gcgcgacgtg gtggacatgc tgctgctcgg cggcatctgg
     9721 ggcggtgcct tcgtgctgct gcgcgtggca tcgcccgagt tcggcgccgt gccgctcgcg
     9781 gccgtgcgca tcctggtggc ggcgttcacg ctgctacttt tcctgcgcga tcgcagcctg
     9841 ctgcgcgccg agccgctgcg cctgctgctg ctcggcctga tcgcctcggc gattccgttc
     9901 acgctgtacg cctacgcgat gctcagcatc accagcggcc tcgccgcatt gctcaatgcg
     9961 accacgccga tgttcggtgc actggtcgcc tggctgtggc tcggcgagcg attgtcggtg
    10021 gcgcgcgtcg tcggcatcgt gatcgccttc gccggcgtcc tgtggctcgt caacgacgcg
    10081 ctcggcacgc tcggcgtcgg cgcactcggc ggcgtactgg ccgcgctgct gggtgcggcc
    10141 tgctatgccg ttgcggcgag ctacacgcgc cgcgtattcc cgaccgccga tccggtggcc
    10201 ctcgccaccg gcaccgtgct cggtgcgttc gtcgcgatcc tgccgtttgc ggtggtgaca
    10261 tggcccgccg cgccgatctc gctgcgggca tggatggcgg ccgttgcgct cggcgtcgta
    10321 tgcaccgcga tcgcgtacaa ggtctacttc cggctgctgc gcaatgtcgg tgcgggccgg
    10381 gccgtgaccg tgaccttcct gtttccgccc ttcggtgtgc tgtggggtgc gctggtactc
    10441 gccgagccgg tgaccctcag cctgctcgca ggctgcgcgg tcgtgcttgc gggcacggcg
    10501 ctggccgtcg ggcttttcga tcgtgtgaga tactgaaata tgagccagat ccgcaacctg
    10561 atcaccgccg ccagcgtcat cgtcgcgctg ctggcgggtg gctgggcgat ctacgccacc
    10621 cgacacgccg gcgaccgggt ggcgcccgtc gacctggcgg cgggtccgcg cagcgatagt
    10681 gctgtacagg gtgccgcggt ggcggtttcc gtcgtcacgg gcgaggtgcg cagtgagcgc
    10741 atcacgcgcg aattgcaggc gctcggcacg gcggtggcca acgagtcggt cagcatcacc
    10801 tcgaaggcca cgaacctggt catggccata aacttccagg acggccaggc cgtgaaacgc
    10861 ggccaggtgc tggtcaagct cgacgatgca caggcccgtg ccgatctcgc cgaggcgagc
    10921 gccgcgctga ccgagagcac gagccagtac gagcgggcgc gcgacctgct ttcgacgcgc
    10981 gtcctgtcgc agtcgcaata cgaacagctc gaagccacga tgaaggcgaa ccgggcgcgc
    11041 gtcgacgccg cgcaggcccg gctggacgac accgtgatcc gcgctccctt cgacgggcgg
    11101 gtcggcttgc ggcgggtcag cgtcggcagc ctgatcaatc ccggcacggt gatcacgacc
    11161 ctcgacgata cgagcgtcat gaaggtcgat ttcgcggtgc cggaaaacca gatcgggagc
    11221 ctccgcagtg gacttgcgct cactgcgcgc accgccgcgc atccggaccg gctcctcgag
    11281 ggtcgcatca tgaccatcga ttcgcgcatc gatccgacca cccgttcggt cacggtgcgg
    11341 gcactcgtgc cgaacgacag cggcctgctg cggcccggca tgtacgtgaa cgtgtccctc
    11401 gcgcgggagg agtacgaggc gctcgtgatc cccgagcagg ctctggtgcc ggaacagagc
    11461 cgccagtacg tcttcgtcgt cgaggacgat cgggtcgcga ggcgcgaggt gcagatcggc
    11521 cggcgaacgc cgggcagggt cgaggtcgtt tccggtctcg ccgcgggcga acgcatcatc
    11581 gtcgagggta cgcagaaggt gcgcgaaggc agcctcgtcg ccgaggctgt caaccagccg
    11641 gtctgaacat aagctgttga atttcaagaa ttaagttcgc catcgaggcc ccgggcggcg
    11701 ccccagcggg tacaatggca gacgttcccc cgtctggtga caccgtgtcc gcaacgccct
    11761 atcagcagct cgagcaggag tggaagcgcc tccacgcgtt ccgcggtgcg ctcgcgctgc
    11821 tgcgctggga cgccgccgtg atgatgccga agggcagcgc ggagaaccgc ggcgagcagc
    11881 tcgcggcgct cgagaccgaa taccacgcac tgctgacctc gccgcgcatc agccgcttgc
    11941 tcgaccgggc ccaggcgaac gtgcagggct acaccgactg gcaggtcgcc aacctgcgcg
    12001 agatgcgccg ccagcgcgac cacgcgatcg cgactccggt gtcgctcgtc tcgcgcctgg
    12061 cgcgcgccac ctccagggcc gaggtccagt gggtcgaggc acgccgcgcc gacgatttcg
    12121 cgatcttcgc gccgcatctc gaggaggtgc tgcacctcgt gcgcgacaag gcgcagctgc
    12181 tcggtcaggc gctcaatctc gccccttacg atgccctgat cgacgaattc agccccggca
    12241 tcacgatggt ggacatcgac gcgatgttca aggcgctgtc gcgccgcctg ccgaccctga
    12301 tccgcgaagc gatggcgctg caggagaagc atccgccggc gcccctgacc ggcaagttcc
    12361 cggtcgcgaa gcagcgccag ctcgtgaacg agttgatgaa ggcgctcggc ttttcgttcg
    12421 agaccggccg catcgacgag agcgagcatc cgttcaccga gggcgtcccc ggcgacatcc
    12481 gcatctcgac gcactacagc accgacgatc tgttctccgg cgtgctcggt gcactgcacg
    12541 aaaccggtca cgcgatttac gcccacgggc tgcccagcga atggcgcgac cagccggtcg
    12601 ggcgcgaccg cggcatggcg atcgaggaga gccagtcgct gctggtcgag atgatcctgt
    12661 gtcgcagccg gccattcgtg cgctacctgc agccgctgct cgtcaagcac ttcggcgtcg
    12721 agggtcccga gtgggaggtc gagaacatct acgcccacct gacgcgtgtg cggcgcggcc
    12781 tgatccgcat ggatgccgac gagctcacct atccgctgca catccagttg cgctacgaac
    12841 tcgagaagaa gctgctttcc ggccagctcg cggtgcgcga gctgccgctg gcgtgggccg
    12901 atttcatgtc ggggcgcttc ggcgtcacgc ccgccaacca ccgggaaggc tgcctgcagg
    12961 acatccactg ggccgtcggc tcgttcggct atttcccctc gtacgcgctc ggcgcggcga
    13021 tcgccgcgca gctgtacgag aagatgcgca tcgacattgg cgccctcgac gagcagatcg
    13081 cgcgcggcga gttcggcgcc ctgatcggct ggctgcgcga gcaggtgcat tcgctcggtt
    13141 ccaggctcac gatccaggag ttgctgaagc atgcgaccgg caagccgctg tcggcggcgt
    13201 ccgcattgcg ttacctcgaa ggcaagtacc tcgaggatcc ggcctcccgc accgccgccg
    13261 cctgacgtga ccgcgccaca gcccgtcgat tccaacacct cgaagcggct cgccgcgcac
    13321 ctgcgcggcc tcgccggcaa ggcgatcgag gatttccgca tgatcgaggc gggcgaccgg
    13381 gtcatggtct gcctctcggg cggcaaggat tcctacactc ttctcgacct gctgctttcc
    13441 ctgcagcgca gcgcgccggt ggatttcgag gtggtcgcag tcaatctcga ccagaagcag
    13501 ccggatttcc cggcccacgt gctgcctgac tactgccgtg ccctcggggt gccgttccac
    13561 cagatcgagc aggacaccta cagcgtcgtg aagcgcgtga tcccggaagg gcgcaccatg
    13621 tgcggcctgt gttcgcggct gcggcgcggt gcgctgtacc ggttcgccga ggagcacggc
    13681 ttcacgaaga tcgcgctcgg acaccatcgt gacgacatcg tcgagacgct gttcctgaac
    13741 ctgttccacg gcggacggct gaaggcgatg gcgccgaagc tcgtgtccga ggacggccgc
    13801 aacgtggtga tacgcccgct ggcctacgtg ccggagcgcg agatcgagcg ctatgcgcgc
    13861 gcccgcgcct ttcccgtgat tccgtgcagg ctgtgcggtt cgcaggacaa caaccagcgg
    13921 gtggccgtga agcgcatgct cgccgcgtgg gagcgtgagc accccgggcg cgtcgcgtcg
    13981 atcttctcgg cgctcaccca ggtcgagccg gcgcatctcg ccgatcccgc cttgttcgat
    14041 ttcgcatcga tcacccgcgc gccgcagtga tcgagctgcc gttcagcaac gcctactggg
    14101 tacgggaagg gcgctggctc gcaggcgaat accccggtgg cccgacgcgc gaggacacgc
    14161 gcgcgcggct cgcggcgctc gcctcgctcg gcatcgacac ctggctcgac ctgacttcac
    14221 cgggcgagct gcccgcgtac gagggcgagc tcacgcgcgg gctgctgcac atcaggaagc
    14281 cgattcccga tcacggcgtg ccgctcgagc ccgagcacat gagcgacatc atcgaggtca
    14341 tcgatggcct gctcgccgac ggtcgcaacc tatacctgca ttgccgcgca ggcatcggcc
    14401 gcaccggcat ggtggtcggc tgctatcttg cgcagcatgg ccggcgtggc gacgcggcgc
    14461 tcgatgcgct gaatgccctg tggtcgggca gcgcgcgtgc gcgttcgtgg ccgtcgatcc
    14521 ccgagaccga cgagcagcgc aatttcgtgt accgatggcc cgcgatcctc gatcccacgc
    14581 tcgacgacga ggtgatggcc gcgacccacg cgctgcggga gcgcttcagc ggcgcgctgc
    14641 tcgggctggc ggtgggcgat gcgctggcgg ccgcgacgca gtaccgcaag cccggcagtt
    14701 tcacgccggt gggtgacctg cttggcggcg gcccgttcga cctgccgcgc ggcgcctgga
    14761 gcgacgacac ggcgatggcg ctgtgcgtgg ccgacagctt cctcgagcgc ggcggtttcg
    14821 atccggtgga ccaggtcgag cgtttcatgc gctggcagcg ggacggctac ctttcggcaa
    14881 ccggccagtg cgtcggcatc acggccggcg tgacgcgtgc gctggcggcg gcgcgctggc
    14941 gccggcagcg ctttcccggt tcgcacgatc cggcgctgct cgatccggag ccactgaccc
    15001 ggatggcacc ggcgctgatg ttccacttcg ctgatcccgc ggtcgcgatc gatacggccg
    15061 tcgagctcgc gcgcaccacc tgccaggctc cgctcgtcct cgaggcctgc cgcgtgctcg
    15121 ctaccgcgct gcacgcggcg ctcagcggcc gcgacaaggc cgtgatcctg cacggcgtgc
    15181 gcctgctcgg ccggggcggc ggcctgcgcc ccgaggtggc gcagatcgcg gcgggcggtt
    15241 atcgcaatgc actcgcagct cccgcacccg cccaggccac cgcgctggat gtgctcgggg
    15301 cgacactggg ggctttcgag acgacgtcga ctctgcgcga ggcattgctg ttgtgcgcca
    15361 atcgagggcg cgacgcggat gtcctgaccg ccgcctgcgg gcagctggcg ggtgcccact
    15421 acggcgcggc ggccattccg gcggcatggc gcaacagcct gatcggcaag gaaatcgtga
    15481 gcgatttcgc cgaccgcctg ctggcccggg cgctggaagg actggcgggg taacgcccgg
    15541 aaggcccggc accgactgct atacttttca ggctcctctc cgctatgtcc gtgccggccc
    15601 cgcaggccgg cgcaccgagg ttgtctgtcg atgccgattc tcgtccaccg tcgtcccgcc
    15661 gcccgcgcgc gcgccaggtc gccgcgcaag gccgcgccag cgcgccgcaa cctcaagtgg
    15721 gcgcgcttgc gcgacgagga cctcctgcag atgcgcttct gcgacctgaa gctcaagctc
    15781 gaccgtacgc cgctcgcgcg ccatatgcgc acgctgtatc gcgatctcga acgtcgcggc
    15841 atcgacttcc gtccgcatgc ttggttcggt gaggagtggt tttcgccgga tggcgtgccg
    15901 ggcatctcga tcccgttcta tctcgcgcac ccgcgcctcg agcgcctcga gcgcacgatg
    15961 atgaagaatg tcgagggcgg caacgcgcag tggctcaagc ggatcctgcg ccacgaagcg
    16021 ggccatgcga tcgacacggc ataccggttg cgccgacgca agcgctggcg cgaaaccttc
    16081 gggccggcct cgctgcctta cccggatcgt tatcgcccgc gacccggcag ccgtcgctac
    16141 gtgcagcacc tcggcgactg gtacgcgcag tcgcatccca ccgaggattt cgccgaaacc
    16201 ttcgcggtct ggctcacgcc gaactcttcg tggcgccgtg gctatgccga ctggcccgca
    16261 taccagaagc tcgagttcgt cgacgaattg atggccgggt tgcgcggcaa gcgcgccccc
    16321 gtgcgcagcc gcgcgcgcat cgagccgatc gataccagca cgcgcacgct cgccgagcac
    16381 tacagcgaaa agctcgagcg ctacagcgag gatcgccgcg ggctcatcga cgtgatgctg
    16441 tcgaaagtgt tcatgccgag ccggccgcga cgcacgacgc cgcgcgcgag tacgctgatg
    16501 cgtgccgcca ggcgggagct cgtcgacgcg gtagtgcgcg aacagggagt tgcgcgctat
    16561 agtgcgttcc agatattgcg catgatcatc gaacgaagtg atcaattggg cctctatccg
    16621 cgcggcagcc ggcgcgacgc gttgcgcaat gcccgctgga tgctgtcgcg cctcgtcagt
    16681 ctctatgcgg aaagcaccag ccctcaaatg aggctatgaa gaaactcaag gtgctggtcg
    16741 tcgtccacgc gaccctcgtt ccgcccgatt cgctcaacgg gcggagcgaa aaagaaatcg
    16801 aagagtggcg caccgagttc gatgtcatct cgacgctgaa gaagcgtggc cacgaggtgc
    16861 gctgcctcgg cgtgctcgat acgctgagcg aactgcgcgg cgcgatcacc gactggaagc
    16921 ccgacatcgt cttcaacctg ctcgaggagt tcgacggcat cgtcacctac gaccagcacg
    16981 tcgtcgcgtt cctcgaactg atgcgccagc cgtacaccgg ctcgaatccg cgtggcctgc
    17041 tgctgtcgcg cgacaagtcg atctgcaagc agctgttcgc ctaccaccgg ctcccctcgc
    17101 cgcagttcgc ggtgttcagg aaaggagtca agttcaagct gccgaggaag ctgaagttcc
    17161 cgctgttcgt caagtcgacc accgaggacg cctcgctcgg catcgcccag gcgtcggtgg
    17221 tcgaggacga ggcgaagttg cgcgagcgcg tcggcttcgt gcacgagcag atcggctcga
    17281 acgcgctggt cgaggaatac atcgagggtc aggagctgta cgtcggcgtg ctcggcaacg
    17341 accgcctgac ccgtctgccg gtgtgggaga tggtgttcgg ctcgatgcct gattcgctgt
    17401 cggcgatcgc gacccgcaag gtcaagtggg accgccgata ccagaagaaa tacggcatca
    17461 cgacgcgcgc agcggacgaa ctgccgcgcg agatcgtcag ccgcctcgat tcgctgtcgc
    17521 ggcgcatcta ccgcgcgctc gggctgtccg gttatgcgcg catggacttc cggctcgcgc
    17581 cggacggccg gatattcgtg ctcgaggcga atgcgaaccc caacctcgcc gcggaggagg
    17641 acttcgcgct ctccgcactc gaggccggac ttgagtatcc cgagctgctc gagcgtatca
    17701 tcgcgctcgg gctcgcttac cgggcggaat ggcgggtttt ctatggttga acgtggcctg
    17761 gcgctcatca tgctcgcgct gctcggtgcg tgttcgagta ccgccgatct gcgcaaggcg
    17821 gaactgtccg aactcgcggc ctggttgccc ggaacctacg tgaacggcgc gcgcgaactg
    17881 gcattcgtgc cgatctatgc gccgttcatc agcgacaacg tgttcttcac cgaggaatcg
    17941 tccacgcagg gcggccgccg cgtgctctcg cagcgcgtga tcgccttcga ggtcgtcgac
    18001 aagcagatcg tgcaggcgag ctatgcgttc gccgaaccgg gacgctggcg cgccgggctg
    18061 tacaacccag acctcttcaa gggcctgatg gagcaggacc tcaaagtcca ggccggttgc
    18121 gagatgctgt gggtgaagga tgtcgatgcc ggcaagttcg ccggtgcgaa cgacaggaag
    18181 cagtgccgcg cgtcgcgcgc gagcggtgca ctcgcgttcc tcgaggtgcg cgccgagctg
    18241 acacccgacg acttcgcccg tgccgagcgt tactacaacg cgggcggcag gctgctctcc
    18301 ggcagcgagg atgacgagct ggagcgctgg cggaaatccg agtgaagctg ttcatctcca
    18361 gggcgatcag gaaactcgac gacacgacgc agaaaatgcg cgcgctcagg gcgccgaggc
    18421 cggcgccgcc ggagaagccg cgtgcggcgg ctcccggcga ggcgggatcc gggcgcgtgc
    18481 gtcacgatga gcgcggcaat gccgtctggg acatcgcggt cgccaccggg gtgttcgcgc
    18541 tcgagagcac atccaggctg ctgaagcgcc tcgaggcccc tgagctgcgc atcgaggacg
    18601 acaaggaact gaagctcgag gacgacaagg gcggcggtta cgatccgtac aaccgccgcc
    18661 gttgacttgc cggccgccta cttcagccag gtgtcgagcc agtcgagcac ctcggcatgc
    18721 cattgcaggc tgttggccgg tttcagcacc caatggttct cgtcagggaa gtacaggaac
    18781 ttcgaggcaa tgccgcgccg ctgcagtgcg gtgaacgtcg cgatgccctg tgcatccggc
    18841 acccggtagt cgttcgcgcc gtggatcacg agcatgggcg tgcgccattc gccgaccagt
    18901 cgtgacggat cccacttgtc gtggttcgcg ggctgttcgt aatagggccc gccgttctcc
    18961 cattccggga accatagttc ttcggtcgtg tagtacatcg acttgttgtc gaagatgccg
    19021 tcgtggttga cgatgcagcg gaagcgctcg ggccagttgc ccgcgatcca gttctgcatg
    19081 taaccaccat aggacgcgcc cagcgaacag gccttgtccg cgtcgagcca gtcgtagcgc
    19141 tcgatcgccg cggcgagtcc cttctgcagg tcgacgaacg gcgcgccgcc ccagttgccg
    19201 ctgatcgaat cggtgaacgc ctggccgtaa cccggcgagc cgtggaagtc gatgaacacc
    19261 accgcatagc cgcgcccggc atagacctgc gggttccagc gataactcca cgcgttggcg
    19321 aagctcgatt gcggcccgcc gtgcacgatg aacgcgatcg ggtatttgtt ccccggcatg
    19381 tagccgtgcg gcttcatcac gtagccgtac acggtctcgt cgttccagcc cctgaagctg
    19441 tactgctcga attccgccag cgcgcggccg ccgaggcgtg cggcgttggc gtcggtgagg
    19501 cgctcgggtt tgccgccgtc gcgcagccac cagaggtccg ccggggccgc aagattcgcc
    19561 cagctgaaca cgatgccgcc gttcgcggcc gcgaactcgg tgacctgacc cgaaccgacc
    19621 agcttcgagg gcttgccgct caccgggtcg atggcgaaca gcgcacgctg gccgatttcg
    19681 tcggccgtcg cgagcaggcg cttgccgtcg cgcgtggcgc cgaggcgcgc caccgagcgg
    19741 tcccagtcgc gcgtgagcgc cctggcctcg cggcccgcgc gacgaagcat gacgtggaag
    19801 cggtcggcct cgaagcccgg acgctgcatc gcgagccacg cgagatcacc gttcgccagg
    19861 aacaccgggc tcgtatccca cgcgggattg tccgcggtga gattcaccgg cgtgccgccg
    19921 tcgatagcca cctcgaagag gtcgaagttc gtcgaccacg gttcggtgcg gcccgcgatg
    19981 cgcaggctga acaccacgcg ctcgccgtcg ggcgagatcg catactcctc atcgccgccg
    20041 aagggcttcg agggcacgtc gccgtcgagt ccctgcgaca ggtcgatcgg tgtgccgatg
    20101 cggccgctcg catcgagcgc cgccgagaac aggtgcgagc gcgagccgtc ggaccaggtg
    20161 tcccagtggc gcatgaacag cttgtcgtag acgaggccgc tcgccttgct gctgcccttc
    20221 ctggcttcct ccggcggctt gaccgccatg ctgaacacga ggcgatcgcc ggtcggcgca
    20281 atacggaagc tgccgacatc gaccggcagg tcggtcaccg cgagcggctc gccgccgccg
    20341 agtgcgaggc gccagatctg cgcgctgccc gagcgcgtcg agaggaaata gagcgcgctg
    20401 ccgtcgggcg cccaacgcgg atgcgtgtca ccggcggcat ggtgcgtgag cttgcgcggc
    20461 gccgggttcc gcgcggagag atcgagcagc cacaggtccg tgcgtccgcg gttggcttcg
    20521 cgatcagttt cgcgcatcac gtatgcgacg tggcgaccgt cgggcgacac ctggggatcg
    20581 gatatgcggt gcagtgacac gaggtcatcg gcggtgaagg gcgcgctggc gagcgcggcc
    20641 gtcgcggcca gcccggtcag gcacagggtg agtgcggcag gtttcagcat cgcggcctcc
    20701 ggcatgaatc gagggccgca atgataccca agcgccctca gccgatctcc gcgtgcggca
    20761 atacccaggg ttcggcctcg gccgcggaca tccactcgcc caggatcgga tcaaggagca
    20821 cgtgcgtcac atatgcggcc gcggccgccg acagcgccac gccgtcggtg cgcgcggcgt
    20881 aggtgttgaa gcgcagcacc accggcgcga acatcgcgtc ggccacggtg tagtcggcgc
    20941 cgaacagcca cggcccctgc gcggcgtgtt cgcgccggca ctccgaccat gccgcatcga
    21001 tgcgcgcgat atcggcggcc aaccccggcg tcatggccgt gcggcgattg cgggcgcgcg
    21061 agttcatgcc ccaggcactg cgcagttcgg tgaatccgga gtgcatctcg gcggcgagtg
    21121 cacgtgcccg ggcgcgcgca gcccgctcgc gtggccagcc gcgcccggcg agttcgcagg
    21181 cgtactcgca gatcgcgagc gaatcccaga tgacgagttc gccgtcgcgc agtaccggca
    21241 cccggcccgc cgggctgaag gccgccacct gcgcggcgaa ctgcggcgag tcgagctgca
    21301 ctttgtccgt ggcgaattcc agcccgaggt tgcgcagcaa catccacggg cgcagcgacc
    21361 aggacgaata gttctggttg ccgatcagca gcgtcaggtt cattgcagtt tcatccctcg
    21421 cgtttcgatc accagcgggg tcagcattgc tgccgcgaac ggaatcaccg ccacccagat
    21481 catcgtgccg aggagcgcgg ccgacgagcc gtccgccgcc gaggtcacga tgccgatcgc
    21541 cagcgggccg gccgccgcga cgacgcggcc gatgttgtag cagaagcccg cgccgctcgc
    21601 gcgcaggcgc gccgggaaca gctctggcag gtagaacacg taggtgctga agattccgta
    21661 tacgccgagg cccacgaaga acagcaggcg gatgcgcagc tcgggcgcga actcgagccc
    21721 gaaggtcgcg acgatggcga acgcggaata gacgaaatac gcggcgaaca tcgcgcgccg
    21781 cccgagccag taggcgagcg gcagcgccgc cagcgcaccg acgaggccac ccaggttgaa
    21841 ggcattcgat gctatcgtct tccagcgctc gatctcgccg ggtgcgttct cgacggcgag
    21901 ccaggcgccg agcagcggca cgaaggcatt gcaggcccac caggtcagca ggccggcgac
    21961 cgacaccagg gtgccgacga gtgtcgcgcg acgcacctcc ggcgtgaaca gctcgcgcgg
    22021 cgagggcggt gcactgtgcg cgcgcgcgtg cgcccaggct tcgggatcgt ggatgaaggc
    22081 gcgcacggcg aacgccagcg cgacgggcgc gaggccgcag aggaaaacgt agcgccagga
    22141 attcgccggg tcgtcggcga accacacgcc cgcgacctga tagttcacga ggccggcgag
    22201 cagcagcccg atgggcgagg cggtgtagag cagagtgcca ccgaacacgc gccggttctc
    22261 cggcaaggtc tcggcgacca gcgcggcgcc gacggcccac tcgccgccga taccgaggct
    22321 cgcgatcgcg cggaacacga ccagctgccc catgttcatc gaggcggcgc acagcgccgt
    22381 gccgatcgcg tagagggaca ccgtgacgaa cagcgcgcga cggcgcccgt agcggtcggc
    22441 ataccagccg aacaggatgc cgccggcggc ccatccgacc agcaggatcg aggagaggat
    22501 gccggtccac agcaccgttg catcgcgtgc ctcggagccg ccgggatcga gcccaagcag
    22561 cgtcggcacc gtgttcggtg cgacgaagtt gaaaagcagc gcatcgaaaa cgtcgaagcc
    22621 ccagccaagc caggcggcag ccagcaccag ccactgatat cgcgtcatcg ttcgtgaccc
    22681 ccgtcgcaat cagcagtgta accgcatggc tgccaggcgc ggcgctgcgg ctgcgcgctt
    22741 gaaactctgc gcccgttcac ggattgttca ggcgcacggt aggaggctag gacacatggt
    22801 cgtcacggta cacagcctgc gcgagtactt ccgtgaagcc ctgcgggaga ccctcgcccg
    22861 acagcacgtc gaggtcggcg gcgagaccga gcagtacgtc gtgaacatgc tgacgctgtt
    22921 cgcacgttcg gaggcgctgt tcgagcagac gccggagggt ccgcgcatca agccgctggt
    22981 cgtcatgctg ggcgaggcgc tcgaagcggg cagcgcccgt gaacgttgcg ccggcctgca
    23041 gcggctcggc gacgtgtcgc tgttcgtcgc gggattcatg gcgcagggct tcgcgcgcaa
    23101 gctggtcgac atcgactatc acatcgcgat gggcgggcgc gcctacggga cgctctcgga
    23161 cgcggtccgc cagagcgggc ggcccgcgct cgggccggtg ttcggcgagc tcgccgcgaa
    23221 gttccagctg ctcgtcgatg cgctcaacga ggtgagcgaa tcgggctacc agcacagcga
    23281 tcgggacatc ctgcgactct acgagctgtg gatcaaaacc ggcagccggc gtgccgaacg
    23341 cctgctggcg aaactcggcg tgtcgccggc ggccggcgca cgttcctcgt tccagcattg
    23401 acgggagcgg ccatggtgct gcgcgccctg caggatctgc tcgccgccat ctacgatgtg
    23461 ccgctcgcgc acgacgtgta cgatttcctc gtcactgatc caggatgcgt gccgggcgcg
    23521 gagcgttccg cggccaacga ggaacagttg ctggtggccc gtgacggcga cgacatggcg
    23581 gtcgcgctgt tcctcgatgc gcagctgctc gaacgcctgg ccgccgccga cccgctcgaa
    23641 cgcctggatg gcggcaacct cgccgactgc tggaccgcgc tcgagggcgt gagccacttc
    23701 cactatctcg cgttccacgc cggccacgac cgcgaggtgt cgcgcatgga gctcgagacg
    23761 caggccgaag tcgacaagta tgtcgccacg ctctggctgc tgcgcagcca gtcgccggag
    23821 cgcttcccgc gcgagctgca ccgtatcctg ttctcgtgca cgcaggtcga tccgcggctg
    23881 ccgccgcagc gtgcgcagct ctaccggcgc gcgagcgcct acgcggcgcg cttctgccgg
    23941 cgcatggagc acaggctgaa gcatccggcg gacgcggcgt tcagggatat ggtggcggaa
    24001 ctgcgccggt tctaccggct caccgaggcg cgcaagctgg cgcacatcga gcgttgcgcg
    24061 taggcgctac tcgtcgtctt cgccgcgctg tttgcgctct tccatctcgc gcttcaactt
    24121 gatccactcg gagagtttgc gcaggtcgcg gcgcgtggtg ccggtgccgt gggaatcgcg
    24181 cctgccggac ggaacggtgc gatcgtacgg atcggcgccc atgtcgggcc cttcgccctc
    24241 gatcgtgaat gtcgggcgcg cgaagttccc ggtgcggtcg tccgggacct tccgccattc
    24301 gacgcgggca ttgccgagat cgtcgtggac gacctgggcg acgcggcgcg cgccgctggg
    24361 cggtttgtca gaatctcctg ccacgatcgg cgggagtcta gcgaacgcgg ggcgtcggaa
    24421 cccggccgca gttcacatcc cgcccggtct gcagttcaca tcccgctcgg tccgcagttc
    24481 acatcccgcc cggtccgcag ggcggtgagc gcggggtgtc ccgctgcgcg cagcaggcct
    24541 acgcaggtct tggcatcggc gaattcgccg gcgagcacgc ggcggcaggc gtcgtcgatc
    24601 ggcagccagt gcacctccag cagctctccg gactcggtgg cctgtgcggc aggctcgagc
    24661 gccgtggcga gaaacaggtg gacccgctcg gtgagcacac ccggcgaact gaggatggtg
    24721 ccgagcgaat cccaccgggt ggcgaggatg ccggcctctt cgacgatttc gcgctgcgcg
    24781 gtggcaagcg gcagctcgcc gggctcgagc ttgccggcgg gaagctccca tatccagccg
    24841 cccgccgcat gtcgatactg gcgcagcacg cacacccgca gcgctgcatc gaccgccacg
    24901 atcgccgcac cgcccgggtg atgcacgatg tcgagcgtca cgcgatgacc gttcggcagc
    24961 tccaccactt cggtactggc ggtcacgatg cgaccgcgga atttcgtttc gcttgaaatc
    25021 agcaccgatc gggcccgctg ctgtcgcgcg cggcgacgct caccgcgtgt cgacctcatc
    25081 cacgtcgagc tgcccggtca gacggcggcg gatgtgcgac caggacatgc cgatcgcgag
    25141 cacggccgcc gcgacggcga ggccgatcca cagcagcgtg cgcggggccg cctcgaacca
    25201 gccgtactgc acggcgaccc agaccagcgc cgcgaagaag gcgacggaaa gcagcacgcc
    25261 gatcgcgccg agcgagcgcg cggtggccgt gaggaacacg acccagccga tcagcagcac
    25321 gacgccggcg agcccttgca gggcatcgaa gttcgggaaa tcgcccgcca cccagtgata
    25381 gtaggaaacg ccgcccgggt tgtagctgag cagcacgagc gccagcgcga gcgcgaagcg
    25441 gatcgccacg ccgccgatgc cgatggactc ctgagccatg cacgcatgat caggcaaacg
    25501 gcggggcggg acaaccagcg ccgcggaaag tatattgcgc gatcatgtcg acgaccgcgc
    25561 atctcgccaa gctgaacgtt gcccagcgca aggccgtgac ctacggcgag ccggcggagt
    25621 cgcagggcta caggtccggg ccgttgctga tcatcgccgg cgccggcacc ggcaagacca
    25681 gcacgctcgc tcatcgcgtc gcgcacctcg cgatgcacgg ggtcgacccg gcgcgcatcc
    25741 tgatgctgac cttcacgcgt cgcgcggcgc tcgagatgcg ccgccgcgcc cacgaagtcg
    25801 ccaaggccgc actgaacgac gtgatcggcg gcgtcagcca gacgatcctg cagcgcctcg
    25861 gctgggccgg cacctttcat tcggtcggca accgcctgct gcgccactat gcgcgccatc
    25921 tcgggctcga cccgcagttc tcggtcatcg accgggcgga ttcggccgac ctgatcgacg
    25981 gcatccgcca ggaactcggc ttcgcctcga aggagcagcg cttcccgcgc aaggacacct
    26041 gccttgcgat ctactcctat cgggtcaaca cgcagttgtc gctcgaggac tcgctcgagc
    26101 ggcagtttcc ctggtgcggg cagtgggcag acgagctgac gaaactgttt cgcacctatg
    26161 tcgagcgcaa gcagaagttc gcgatcctcg attacgacga ccttctgctc tactggcata
    26221 tcctgatgag cgagccgaag ctcgcgcagc acgtggcgac ccacttcgac cacgtgctcg
    26281 tcgacgagta ccaggacacg aaccggctgc aggcggagat cctctacggc ctgtgcccgg
    26341 acggcaacgg cctcacggtc gtcggtgacg atgcgcaggc catctattcg ttccgcgccg
    26401 ccaccgtcga gaacatcctc gagttcccgg agcatttcca gccgcgcgcg gaaatcgtcg
    26461 cgctcgcgca gaactaccgc tcgacgcagg ccgtactcga cgtgtcgaac gcgctgatgg
    26521 cggaggcgcc gaggcagttc cgcaagcacc tgctgtcgct gcgcggcagc ggctcgcgcc
    26581 cgcgcctcgt caccgtggag gacctgccga cgcaggccga gtatgtctgc acgcaggtgc
    26641 tcaaggcgcg cgaagcgaac gtgccgctca ggcgccaggc ggtgctgttt cgctcctcga
    26701 gccacagcga cctgctcgaa gtgcaactct cgaagcgcaa gatcccgttc atcaagtacg
    26761 gcggcctcaa attcctcgag gctgcgcacg tcaaggacct gctcgggatc ctgcggtgga
    26821 tcgacaatcc gcgcaatgcg atggcgggct ttcgcgtgct gcagttgctg aagggaatgg
    26881 ggcccgcgaa cgcgcgccgc gcgctggacc acctgttgca gcagggtggc agcttcgagt
    26941 cgctgcgcag tttcgagccg cccgccgaca gcgccgtcga ctggcgcaag ctggtcgacc
    27001 tgctcgtcac gctcgccgat ccgcagcagc cgtggacggg gcaggtgaac caggtgcgca
    27061 actggtaccg gccacacctc gagcgcctct atgagcacgt gcacacgcgc atcggcgatc
    27121 tcgaccagct cgaactgctc gcgggccagt acccgtcgcg cgagcgattc atcaccgaac
    27181 tcacactcga tccgccgcag gcgagcggcg atctcgccgg gcagccgctg ctagacgagg
    27241 actacctggt gctctcgacc gtgcattcgg ccaagggcat ggaatgggac acggtctatg
    27301 tgctgaatgt cgtcgacggc agctttccgt cggaattcgc caccgggcgc gccgagacga
    27361 tcgaagagga gcggcggcta ctgtacgtgg ccatgacccg cgccaaggac gacctgatgc
    27421 tcgtgcagcc gctcaagttc ccgctgaccc accagccgaa gcagggcgac gcgcacgtat
    27481 acggcgggcg cagccgcttc ctgaccgaga aggtgctgaa gaccctcgag gcgaccacct
    27541 tccagggctc gaacctcggc gagccctcgc tcggtgcggc cggccagacg acgctcgacg
    27601 cagtcgagcg cgtcaagagc atgtggtgag cgccgccctg cggcggcgcc agcgcgccta
    27661 atttcccgaa ggcttgatga acttcagtgt gaagcggtcg ctctcgccga tcgccgcgta
    27721 cttttcgcgg ttctcatcgc cggcggcgaa tcgcggcggc agcgtccaca cgcccttggc
    27781 gtaatccttg gtgtccttcg ggttcgcgtt cacttcggac ttcgccacga gccggaagcc
    27841 cgccgcctcg atcatgcgga tcgcatagtc ctcgttgacg taaccgttcg ccgctttcgg
    27901 atcctgcggg accgcgggat tgccgcgatg ttcgacgatg ccgagcacgc cgcccggctt
    27961 cagcgccttg tacatcgccg cgaaagcctc gggcgcgaag ccggcggcca tccagttgtg
    28021 gatattgcgg aaactcacga ccaggtcggc gctgccgggc ggcgcgatct caccgacgcc
    28081 ggggccgagc gcggtgacgg tcgcgctgcc gtacagctcc ggacgggccg cgagcttgtc
    28141 ggcataggcc ttgagcgagc gggtgacgcc cgcgctgccc ggattcggag gcgtgtgggc
    28201 ggcgtagtac ttgcccttgc cttcgaccag cggggcgatc acttccgtgt accagccgcc
    28261 gcccggccag atctcgacca cggtctgttc ggggcggatg ccgaagaaca gcagggtctc
    28321 ccgcggatgg cggtagacat cgcgcgcacg gttctccgcg ctgcgatgat cgcccgcgag
    28381 gatgcggtcg agcgcggccg cggtgctgct gcgagtgctc gtcgtcgttg cgcaagcggc
    28441 cagcgcgagg acgcctgcca tcgcgaccat catcgacctg atcccgtgag ccatcggaaa
    28501 tccccctgta ttggaggccc taccgtagcc cgcaagcaac gggaggtaaa gtccgggcgg
    28561 gggcgagatc ctgtggggtc agcggcggta cggcccgccg ttggaccgcg gccgctgcgg
    28621 gcgctcctgc gcttcgttga cgcgcagcgt ccggccgttc atctcgcggc cgttcatcgc
    28681 ctggatcgcc tcccgggcat cgccctcggg catctcgacg aagccgaacc cgcgcgggcg
    28741 gcccgtttcg cgatcgttga tcagcttgac cgactcgacg gtgccgtgcg cttcgaagag
    28801 cacgcgaaca tcctgttcag tcgcagtaaa aggcaggttc ccaacgtaga tcgtcgtcat
    28861 cccgtcatcc cgtccgaaaa ggccgacgga tcgatgctaa ccccgcctcc gcggccaggc
    28921 aaaccggggc cgggcgggcc ggccccggcg cgcatcaatg acgggcggga ggggccggcg
    28981 gcggcacctt cttcatgaaa tacgtccagc tggtctcgtt cgggcgcgtg tcatcgcccg
    29041 gcggcggcga tttgtacttc ggataattcg tctcgatctc gtgctgcagc aggtgcggca
    29101 ggtcggcgaa gcgctcgatc ttgcgcccgg cggcgtggaa ataaacgagg cccggccggc
    29161 cgcccatttc catccacggc agccagcccg actggcgcac ccagccgacc cgcaccgccg
    29221 cggtatcgcg cgcgtcgtcc acgaggtcgg agatatcgcc catgaagttg aacatctcgg
    29281 tcgcgtggta catgccgccg acgtagggct ggtacggccc gccgagcgga ttgttgtaga
    29341 agagcgggat cgtgagggtg agccaccagt tgtcgccgag cgagcgcgcg ggccaggtca
    29401 ccggcttgcc gtcgcggccc acggggaaca tcggcggctg gttcaccgga tcgttgtcga
    29461 tatgcagtac cttgaccgac ttgccagtcc agggattctg ccaggtgtcc agcacctcac
    29521 cggtcttcgg gtccatgtac agcagcagct cgcgcgagac gagccgccag ccggcgccct
    29581 tttccgcatc gttcaccgtg acgcaggtgc ggatgttcat ggcctcgaaa tggaacagct
    29641 tgcgatcggg ctcgccctcg acgcggctgt acacgtcgcc gttccagtag taggtgaccg
    29701 gctcgttgtc cttcagggaa cattgcacct tgcgcatggc gaggttcgcg ccctcgggcg
    29761 tcgcgagatc gatactgccg gcgtgggcgc tggcggtgac tgcgagcagc ccggccgcaa
    29821 gcagcagtcg tgtggggacg gtcgcggcgt ggcgcatcga tgaagccctc ggtgtggtgg
    29881 tggccggtgc atcgtagccg cggggcgggc gcattgccca gccggcactg cccggcggcg
    29941 cggcatgacc acaggacgat cgccggcgcg gctgctcgcg ctgggctcgg gggcgggctc
    30001 aggcgggttc gaggtaggcg tagccgttca gttcgcgttc gtagaagcgc ttcagcgcct
    30061 ggctctcggg gatcgtcatg cgcccggcgc ggatcgcgcg ctcgcagtcc cgcgacaacg
    30121 ccgggtagag ctcctcgacg tcgtactcca tgtactcgag gaccttgttg gcggtgtcgc
    30181 ccttgaccac ctcgtcgatc gtccagccgc cctcgtcatg ctggcggatg tgcaccacat
    30241 gcgtgtcgcc gaacaggttg tgcaggtcgc cgagagtctc ctgataggcg ccgacgagga
    30301 aggcggcgag gtagtactcg tcgccgttcg cgacattgtg cagctcgagc gtgcgcttca
    30361 cgtcgcgcgg cgagacgaag cggtcgatct tgccatccga atcgcaggtg atgtcggcca
    30421 gcacgccctt cctgaccggg cgctcgtcga gccggtggat cggcatgatc gggaacagct
    30481 ggtcgatggc ccagctgtcg ggcagcgact ggaacaccga catgttgcag aagtagatgt
    30541 cggacaggat ggcttcgagc ccctcgagtt cctcgggcgt ttcctcgagc cggcggca
//
LOCUS       DAOQVY010000016        29162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_1889_length_29162_cov_5.771258, whole genome shotgun sequence.
ACCESSION   DAOQVY010000016 DAOQVY010000000
VERSION     DAOQVY010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 29162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29162
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1889_length_29162_cov_5.771258"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1008)
                     /gene="rluD"
                     /locus_tag="PKN91_02860"
     CDS             complement(49..1008)
                     /gene="rluD"
                     /locus_tag="PKN91_02860"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: protein motif:HMM:NF008385.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HNS27042.1"
                     /translation="MTAEFRRHDLQLSPTLAGMRLDQALASALPQYSRSRIQGWIERG
                     TVLVDGRVARSRDRVLGGERVVLEGEVPLDTGTAPQAMALEVVYEDEQVLVIDKPAGL
                     VVHPGAGNRDRTLQNGLLARDPRLAQVPRAGLVHRLDKDTSGLMVVARTIEAHAALVA
                     MLAAREVSREYLAIVTGVMTGGGTIDEPIGRHRAARTRMAVRADGRPAITHYRIVERF
                     RSHTLARVTLETGRTHQIRVHLAHIGYPILGDPVYGVRRRLTAGATPALLEALHAFRR
                     QALHAARLAFVQPVSGEALEFEAPMPADMRGLVEVLRADARGA"
     gene            1106..1867
                     /locus_tag="PKN91_02865"
     CDS             1106..1867
                     /locus_tag="PKN91_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNS27043.1"
                     /translation="MAVLASILAVSGCRTNKQDDYARTNPEVLYQRAHRSMKNQDFNT
                     AILMYEALTARYPFAPESRQARLDLIYAYYRGREAESAIDAAETFIRENPTHPRIDYA
                     WYIKGLVDFERTPNRLERMFNVDLSERPPQTAGRAFASFKTVVEQFPKSEYAHDARRR
                     MVYLRNRLADYELYVARYYMKRGAWVAAAQRAKQTIEQYDGAPAVREALEIMVEAYDR
                     LEMKELAEQARAVYAVNFPAEQSRVEEKKSWWRFW"
     gene            complement(1890..3521)
                     /locus_tag="PKN91_02870"
     CDS             complement(1890..3521)
                     /locus_tag="PKN91_02870"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNS27044.1"
                     /translation="MTRTASLRIALAQIDFLVGDVRGNVARVIETSREAAVQGADLVV
                     FPELSLSGYPPEDLLLHRGLRRQVEEGLAEVCKGVGDVAVLVGLPEYSGAQIYNSMAL
                     VMRGTVQAMHRKSELPNYQVFDEKRYFKAGAQPTVVEVGGVKVGILICEDIWEREAAQ
                     LAQSAGAELLVVSNASPYQLHKQRDRERVAASVACDVGLPLAYVNLVGGQDELVFDGQ
                     SFAMNQAGEVVMRAPAYVEGLYFAEFTRTGAGDIVPLAGAIAPELSEVESVYGALVLG
                     VRDYVTKHRFPGVVIGLSGGVDSALTLAIAVDALGADQVHAVMMPSRYTSSMSLEDAQ
                     AQASLLGVKYSVLPIEAMFEATLATLKDEFAGRPPDATEENIQSRCRMLLLMGISNKT
                     GRMLLTTGNKSEMAVGYATLYGDMAGGFAPIKDCSKRLVYRLAEYRNGLSAAIPQRVI
                     DRPPSAELRYDQKDSDALPPYEILDAILAAFIEEDLSVDEICARGFDRATVGRVLDMV
                     KRNEYKRRQTPPGVRVSRRAFGRDWRYPITNGYRR"
     gene            complement(3518..4618)
                     /gene="sucD"
                     /locus_tag="PKN91_02875"
     CDS             complement(3518..4618)
                     /gene="sucD"
                     /locus_tag="PKN91_02875"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNS27045.1"
                     /translation="MSILVNKHTRVICQGFTGKQGTFHSKQCLDYGTRLVGGVTPGRG
                     GEKHIGLPVFDTVHAAVRQTGATASMIYVPPPYAADAILEAADAGIELVVCITEGIPV
                     NDMVKVKAALAGTGTRLVGPNCPGVITPDECKIGIMPGFVHKKGAIGIVSRSGTLTYE
                     TVFQTTNNGLGQSTCVGIGGDPVRGMNFVDVLELFERDPGTEGIIMVGEIGGSDEEAG
                     AEFIRRFVSKPVVAYIAGVTAPPGKRMGHAGAIVAGGKGTAAEKYRALERAGVRTVKS
                     PADLGSAMHELLFKRRRQRAKAQEIKPRPPLKTKRTVAKAKPRTVAGKRALAQKRPAA
                     KKRVVARKRPVAKKRVVAKKRVAAKRRARKPK"
     gene            complement(4615..5802)
                     /gene="sucC"
                     /locus_tag="PKN91_02880"
     CDS             complement(4615..5802)
                     /gene="sucC"
                     /locus_tag="PKN91_02880"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNS27046.1"
                     /translation="MNLHEYQAKEVFRSYGVPVPEGRVAHSADEAVAAARAIGGAVWV
                     VKAQVHAGGRGKAGGVKLARDLEAVRAAAQGMLGQRLVTKQTGEEGLPINAVYVESGS
                     EIAREIYLSLTLNREKGRIALIGSSAGGMDIEEVAEHTPEKIVTLNVNPAAGLQPYQC
                     REMAFALGFSDRQIGEFQQIATALYRLYLERDASLLEVNPLIVTRAGNLVALDAKINI
                     DPNALFRQKDLAALRDITQEDAMEVKASEHDLNYVSLDGDIACMVNGAGLAMATMDLI
                     KLHGGRPANFLDVGGGATAERVTEAFQLILSNPNVRAILVNIFGGIVRCDLIAEGIII
                     AAKKLGVAVPVVVRLQGTNADKAREMLAGSGLALNPATDLTDAAKKVVALAAQGSNKK
                     VKA"
     gene            5892..7511
                     /locus_tag="PKN91_02885"
     CDS             5892..7511
                     /locus_tag="PKN91_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS27047.1"
                     /translation="MRADRSEDNSTLGDLGWRVVGLLNLYRLLIPPVLLAIHRFTAPA
                     PTVGGTFPTLFHLTCAAYFAAAVALVLAQRLPQIPRRGLILAHAIVDAVAIGLLLHSS
                     GGVASGLGILLVVPVGSVALLAEGRQTLLMAAIAVVALLAQQVIGQIGGTASPADYPS
                     AGVIGVVIFLVALASWPLTTRLRESEALIKRRELDLANLAELSQYIVQHLRESLLVVD
                     QADCIRLINESAARILGDRSAFPGALLGEASPRLLYLLSTWRQSGERPKDSGATFIGS
                     DGASEILPHFAPLGNQDPPPVLVFLEDTTEVASKLQQLKLASLGRLSASIAHEIRNPV
                     GAMSHAAQLLGESPHLSDEDRRLAEIIRANAARVSDIIANVQSMSRREPLRPEQFELG
                     AWLREFRTEFCSTLPCPEDLVELHESGEGLEVRCDRGQLRQITWNLADNAIKHNRAVP
                     HAIELRAGRLSPSQRPFLEVSDRGGGIPEALTERIFEPFVTSEARGTGLGLFLARELA
                     QANGATLIYEPRPGGGSVFRLVFSDPLRWRA"
     gene            7508..8884
                     /locus_tag="PKN91_02890"
     CDS             7508..8884
                     /locus_tag="PKN91_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNS27048.1"
                     /translation="MSRPAVLVVDDEPDLVELVCLTLNRMDLETASAGDVAGARELLN
                     TRHFDLCLTDMRLPDGDGLDLVAWIQENKPLVPVAVISAHGNVESAVRALKLGAFDFV
                     SKPLDLGSLRKLVASAIKLGERADGEMTMRGPRLIGHSAAMDTLRQMIARVARSQAPV
                     HISGESGTGKELVARLIHESGPRAEGPFVPVNCGAIPTELMESELFGYKRGSFTGAVA
                     DSKGMIQSAEGGTLFLDEVADLPLHMQVKLLRVIQEKSVRPIGETREVPVDVRILSAT
                     HKNLAALVRDGRFREDLYYRVNVIEIRVPALRERPEDIPDLAATILRRLSRRMGVEAP
                     DISAGALAVLAAHSFPGNVRELENVLERALTLCITDTIEAGDIQLRAPMLGDPAPATM
                     SAGGSAQQEAAATIGGGALGEQLDSIERDAIVKALEQTRYNKTAAAKLLGMSFRALRY
                     RIKKLGIE"
     gene            8977..9729
                     /locus_tag="PKN91_02895"
     CDS             8977..9729
                     /locus_tag="PKN91_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27049.1"
                     /translation="MKPQWIALAMIWVTSAPAQPTPNVQIELAEIDPAGVPTVAPHTP
                     VYGRIVYTTDAPVRLVLLPYRNGQPVLDGLYNSGSPRYAPGSGETIAWFAFDEPQTID
                     EIRAVANDFYGNVFAEDSIVRNLNWREGAPKPQKAAWVEPLKRAGKDVIVEPPAEYGW
                     FATVVTQLFFLLVPLSVGLQIHAWRKLDGGPGRLARLSGYAMGALWLFVILTGVAGSN
                     LSPIWIVFLSPLFVLFLGALLLVARTRRRRFA"
     gene            9960..10541
                     /locus_tag="PKN91_02900"
     CDS             9960..10541
                     /locus_tag="PKN91_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HNS27050.1"
                     /translation="MLSLQAYSAANLVNGWKISGLKELIHMKTMQKGFTLIELMIVVA
                     IIGILAAIAIPAYQDYTIRSQVTEGLNLASAVKAGVAETFAQTGEWPADMDAAGGDGA
                     NLPSGKYVSSIDVSNGTIIITYGNDANAQLVATPTLTLQPRISANNDVVWLCGYYTGA
                     DVAGLTDPVSGASDAGSTDVTPKFLPQSCRDDT"
     gene            10722..12464
                     /gene="pilB"
                     /locus_tag="PKN91_02905"
     CDS             10722..12464
                     /gene="pilB"
                     /locus_tag="PKN91_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HNS27051.1"
                     /translation="MNDNASLAIGGARSALGGLPQRLVQDGLVDEATMHSALQASRER
                     KSGVVTQLVGMGAAKARDIAVAASHEFGVPLLDLDCLAVDLDLVRLISDKLLGKHRVM
                     PLFRRGKRLFLAVADPTNLQAIDEIKFATGLGIEAIVVEDDKLQRAVDKALEQVDTQM
                     PNLTDEEGVDLEGLEVTGGEDENDGGITRDDIDDAPIVKFVNKVMLDAIRRGASDIHF
                     EPYEKMYRVRYRMDGVLKEVAQPPVQLAQKISARLKVMARLDIAERRVPQDGRIKMKL
                     SKTRAIDFRVSTCPTLYGEKIVTRILDPSQAMLGIDALGYEPQQKELYLANLAKPQGM
                     ILVTGPTGSGKTVSLYTGLNILNREDTNISTAEDPAEINLPGVNQVNVNPKVGLTFAS
                     ALRAFLRQDPDVVMVGEIRDLETAEIAIKAAQTGHLVLSTLHTNDAPQTLTRLIDMGV
                     KPYAIATSCSLIIAQRLARRLCTQCKHLVDVPAEALLKEGFSKEDVAAGIKVYGPKGC
                     QACTDGYKGRVGIYQVLPVTEEIGRIIMEGGNAIQIGDAAKRMGVWDLRRSGLEKVKQ
                     GMTSLEEINSVTIE"
     gene            12482..13720
                     /locus_tag="PKN91_02910"
     CDS             12482..13720
                     /locus_tag="PKN91_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNS27052.1"
                     /translation="MANIAATASVRTIKRDIPFSWEGMDKRGNRVKGKSLAPDEAALR
                     ADLRRQGIAPSKVKKQSQAFKSGGRVKPEDIAVFSRQLATMLASGIPLVQAFEIVGNG
                     HEKPSVQKLILDIKQDIEGGTSLHEALAKHPLYFDDLYVNLVEAGEQAGALENLLDKI
                     ATYKEKTEAIKKKVKKALFYPAAVMVVAVVVTLILLIFVIPQFESLFKGFGAELPAFT
                     QMVINLSNFVQANGIYLAVVFGAAGYAFFYFKKRSRKMREVLDRISLKLPIIGPILVK
                     SAIARYARTLSTMFAAGVPLVEALESVAGATGNVVYENAVMKMRDEVATGQRLQRAME
                     NTSLFPNMVIQMVAVGEEAGALDQMTAKVAEFYEAEVDNAVDSMSSLLEPLIMAILGV
                     LVGGLVIAMYLPIFKLGAVV"
     gene            13717..14547
                     /locus_tag="PKN91_02915"
     CDS             13717..14547
                     /locus_tag="PKN91_02915"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004710834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNS27053.1"
                     /translation="MIAVCFVLGLLVGSFLNVVIHRTPLILERQWRRQCDELAGRETP
                     TEPRYNLVVPRSACPACKAPIKAIQNVPVLSWLALRGRCASCKARISARYPLVELLTA
                     ILSAGIAWRFAPAGLPVVGAALVLTWFLIALTFIDFDTQLLPDSLTLPLLWLGLFASV
                     MGYTGIAPRDAIIGALAGYLALWSVYHLFRLATGKEGMGYGDFKLLAALGAWLGWQML
                     LPIILLSALVGALTGITLIVVRGRDRSVPIPFGPFLAAAGWIALIWGPQIVAGYLGRM
                     "
     gene            14544..15188
                     /gene="coaE"
                     /locus_tag="PKN91_02920"
     CDS             14544..15188
                     /gene="coaE"
                     /locus_tag="PKN91_02920"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNS27054.1"
                     /translation="MSGVFRVGLTGGIASGKSTVANLFAALGVPVIDTDQIARDVVAP
                     GTPLLATLRETFGEDVIAPDGTLDRKAMRARVFGDSQEARAARRTLNELTHPAILAEM
                     EARSQQAGGPYQILAIPLLVEGQSRSRVDRILVVDADEATRIRRLQARDGSTLAEARA
                     LLAAQADRATRLSAADDVIENDGDIAALAAQVERLHAAYLAAAGVYGSGQRQAQ"
     gene            15193..15999
                     /gene="zapD"
                     /locus_tag="PKN91_02925"
     CDS             15193..15999
                     /gene="zapD"
                     /locus_tag="PKN91_02925"
                     /inference="COORDINATES: protein motif:HMM:NF003656.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HNS27055.1"
                     /translation="MSEDPAIRQASLPEEAELVFEQPLNERMRTFLRIDFLYGQATYH
                     NEHASEWSSRAAVASLMDILAITARTDVRADALKELERQLAMLHEFQTRPNVDGSRLR
                     TVMSNLMRLRSELLGTGTGYLQPLKESEFLAAIRHRSAIPGGTCEFDLPDYYYWLQQP
                     AEPRAQAFNEWLALLRPLCDSIAELLWLTRQNGRPREETAKGGVFHITFDRDSPVQLL
                     RISVPQSLGLYPEISGSHYRSSVRFLTWNGLGARPSQATIDVPFTLSCCN"
     gene            15987..16175
                     /gene="yacG"
                     /locus_tag="PKN91_02930"
     CDS             15987..16175
                     /gene="yacG"
                     /locus_tag="PKN91_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HNS27056.1"
                     /translation="MLQLKVACPTCKREIEWNAEAPWRPFCSDRCRLIDLGAWLSEEH
                     AIPGEPAPEADPAPGSRD"
     gene            complement(16178..18325)
                     /locus_tag="PKN91_02935"
     CDS             complement(16178..18325)
                     /locus_tag="PKN91_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNS27057.1"
                     /translation="MNDSQSTRRRGALPLLIVMTITLGAAALAAAGLRYPGSPRGSAV
                     DDYFGTRVADPYRGFEDMDSRATKKWVTAQNALAQPWLEAIPQRAWVKDRLTKLWNYE
                     RYGVPKKAGGRYFYTRNDGTQNQNVLYVSDSASGEGRVLFDPNIASEDATIALARFEP
                     SPDGKLLAYSLSDGGTDWEIWKFRRVDDGVDLPDELRRVKFWQMSWAPDGSGLYYSRY
                     PARADGKGDDAGRPAVYFHRLGEPQERDALVYAVTDHPTRAPSADVTDDGKLLVISIY
                     EDTRRDGVVLKDLTRPDGAVVKLFTDFDANYFYAGDDATTLYFVTSSGAPNWRVLAVD
                     RAAPSAAGARTIIPEAREAIDAVAIAGERLVVNYVKDARNVTRLFERSGKPVGDVPLP
                     GPGSAGNFEAKDGDAEAFFAFSDYLAPTRILRLDTATNGVTAFRTPKVDADTGAFVTE
                     QVFYASKDGTRVPMFITHRRDMPRDGNQPVLLYGYGGFNISLTPVFRPTVLAWLEMGG
                     VYAEANLRGGGEYGEAWHDAGTLTRKQNVFDDFIAAAEYLVREKYTQPSRIAISGRSN
                     GGLLVGAVMLQRPELFGAALPGVGVLDMLRYHTASANARQWSGDYGLSEDAEQFKALL
                     AYSPVHNVRKGACYPPTLVTTADRDDRVVPWHSYKFAAALQAAQGCDNPVLIRIETRA
                     GHGAGKPVWMQIEDFADQWAFAAKALGMPTPAR"
     gene            18383..20638
                     /locus_tag="PKN91_02940"
     CDS             18383..20638
                     /locus_tag="PKN91_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNS27058.1"
                     /translation="MTALRPTRFVALLLWLVAWPAAADELTIERLFSAPDLSGPTLRG
                     ATFAPDGERIAYLRAKADAKDTFDLWAYEIRTRRHVLLVDSARLATAGGALSAEEEAR
                     RERQRTSSLGGILEYDLSADGRRILVPLGGDLYVYDLGAPAERAVQRITQTAAYETDA
                     RFSPRGRYVSFIREQNLHVLELASGREIAITQGGGGLVSYGVAEFIAQEEMNRNTGYW
                     WAPDESRIAYARVDEAPVAEIERFEIHADSVKVVKQRYPATGERNADVQLFVTHIEGG
                     PAARVDLGANPDIYLARVAWFPDARSIAIQRQSRDQKLLTLLKADAASGATRALLEER
                     SDTWVDIVDDFVFVGDRGDFLWGSSRSGYHHLYLYGADGTLKHAVTGGEWMVTAEDRG
                     DSLRAIRGVDSRAGLVYFMANMASPIERQLYVTSYRKPGEPRRISREAGWHTVRMSPD
                     TRYYLDTWSTSEQPPSVALRKLDGSVREALVANTLDATHPYAPYLDAHNPSTFGTLRA
                     SDGQTLYYQLTAPKHLVPGKRYPVIVDVYGGPGVLGRVRNAWGNLFRQYLVQSGYVLF
                     TLDNRGTAMRGVQFDTAAFHRLGSIEVEDQVKGVEFLRSLPYVDPARIGIWGWSYGGY
                     MALMSMMQAPTHFAAGVAGAPVTDFRLYDTHYTERYMGTPAANPAGYAASNVLAHAEG
                     LAGPLLVIHGMADDNVLFTHSTALFKRLQDLGKPFDVMPYPGGKHGLIRHADMGPHAY
                     QSVKRFFDENL"
     gene            complement(20651..21037)
                     /locus_tag="PKN91_02945"
     CDS             complement(20651..21037)
                     /locus_tag="PKN91_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27059.1"
                     /translation="MANDPNLPDIQLDATSLYREEVFTDRKAGTLRRLVPITVDGSDD
                     ATREVLYSGQTQLLTPGGVLPLMFEIDAKTLSEALAKFPDAVKTALEQAIDEAREYRR
                     EAASRIVVPETGGLPPIGPGGKLKLP"
     gene            complement(21080..22942)
                     /locus_tag="PKN91_02950"
     CDS             complement(21080..22942)
                     /locus_tag="PKN91_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNS27060.1"
                     /translation="MTRFAVTRLPVRVVAAALFDARGRVLIADRPPGKHMAGRWEFPG
                     GKVGAQEDERAALERELTEELGIKVIAARPFMTSAHRYPDRDVELSLWLVEDWRGDPR
                     PLDGQQIAWAAPGELGDWDILEADEPFVRALGALTGTDMSTDQNFDVRNPRSGEVDFH
                     FTAPTTAEIGRIAAALRHQQPVWRDAGIAHRVAVMQRWKATLESHKEEIVAALSQDTG
                     RHVIAVGEFGSMLGAIDRWCRLAPTLGAQPDGRSEAWPGVAYGYTLVPYPLVGAISPW
                     NFPLILAFIDAIPALLAGCAVLVKPSEVTPRWVAPVQRTIDAVPELAAVLAVRPGGRE
                     TGEALVANVDAVCFTGSVKTGRLVGVNAAQHFIPAFLELGGKDPVIITADFDPERAAD
                     IVLRAAITASGQACQSLERVYIDRRIHDAFVAALADKARAIDINWPDIHSGYVGPFIF
                     APQARVVEAQIRDALAKGARVLAGGEIENHGGEWLRPTVLVDVTHDMQVMTEETFGPV
                     IPVMAYDTLEEAIALANDSIYGLSAAVLAGSLAEAEAIAHRIEAGAVSLNDCSLTAMM
                     HEAEKNSFKLSGLGGSRMGPAGYMRFFRTKALLRQTGTPATVAVLAEQGAAPAR"
     gene            complement(22939..25752)
                     /gene="secA"
                     /locus_tag="PKN91_02955"
     CDS             complement(22939..25752)
                     /gene="secA"
                     /locus_tag="PKN91_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNS27061.1"
                     /translation="MLALLTRVFGSRNDRLVKNYARTARAITALEPALQALDDEALRG
                     KTAEFRARLKDGASLDDLIVEAFAVVREVARRTLGMRHFDVQLIGGMVLHEGKIAEMR
                     TGEGKTLVATLPAYLNALAGNGVHVVTVNEYLAQRDSDWMAPIYRFLGLTVGVIRNSQ
                     SSAEKREAYACDITYGTNNEFGFDYLRDNLAFRLEDRVQRGHAFAVVDEVDSILIDEA
                     RTPLIISGPAEESTEMYLRVNSLVPRLRPQQEENGPGDFSIDLKQKQVHITEEGHEHV
                     ERLMLEGGLLREGESLYDSGNIRLMHHLNAALRAHAIYRRDVEYIVRGGEVIIVDEFT
                     GRTMQGRRWSDGLHQAIEAKEGVRVREENQTVASITFQNYFRIYRKLSGMTGTADTEA
                     PEFLQIYGLEVVVIPTHRPMVRKDHADLVYLTKDDKFKAIIEDIRDCAQREQPVLVGT
                     TSIETSEEISALLQKEGITHQVLNAKQHEREAHIVAQAGRPGAATIATNMAGRGTDIV
                     LGGNLEAELAALPPEASDADRQRVRAEWQARHDRVVAAGGLHIIGTERHESRRIDNQL
                     RGRSGRQGDPGSSRFYLSMEDSLMRIFGDPNFTKRLLTMAGMKQGEVIESRMLSGRIE
                     KAQRKVESHNFDIRKQLLLFDDVANDQRKVIYQQRTEIMAKDDIADAVRGIRADALGT
                     MVDQFVPKGAGPSDWDLQGLAESVLKDFNTRIAPQEWVQAEPEIDEETFRARIVEAVE
                     AAYEQKVARIGVELMRHVEKDVMLRTLDQHWRDHLAAMDYLRQGIHLRGYAQKDYRYE
                     YKREAFELFSAMLERVKFETASILAKIEVRTQDEIDREEEQRRQRLMRALQAQHQDAQ
                     SALAAGEAAGEAAAAPGGARAAGAGAPAGGVTRLPARAAAPAAESHTPMVRTERKVGR
                     NEPCPCGSGKKYKHCHGALAQAE"
     gene            complement(25884..26804)
                     /locus_tag="PKN91_02960"
     CDS             complement(25884..26804)
                     /locus_tag="PKN91_02960"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNS27062.1"
                     /translation="MNLIFFSRREGRARHLNLAHPVTLGAVGALAVGVLGIAFVLGLE
                     LGQRSGKVFARGDSQHWALQIKQQQAEIANMKNALQERIDALSMRMGQVNGQMIRLDA
                     LGKHLAQMANIDEREFNFDAAPPAGGPEDGAGGVAAQIPDITSMLNDLEARINLRDAQ
                     LAALENVLIARQLREQIHPEGRPVRQGFISSYFGSRQDPFTGLGAFHKGIDFAAQAGA
                     DVVAVAAGIVTWSGERAGYGELVEVNHGNGYVTRYAHNRRNVVAVGQTVQRGDVLAQV
                     GSTGRSTGPHVHFEVLRQGRQVDPLTFIRR"
     gene            26836..27219
                     /locus_tag="PKN91_02965"
     CDS             26836..27219
                     /locus_tag="PKN91_02965"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HNS27063.1"
                     /translation="MPNERNRHKKTGAQTGKSGSARYGKPRPLTDLLARPSSPLTALQ
                     RRAQAAGDWLRFVQAAVATDLAPHVTSAVERGHILTIYVASAAWAARLQFAADDLLAQ
                     AREYDPDLGAVRVKVQPPARATPAR"
     gene            complement(27216..28139)
                     /gene="lpxC"
                     /locus_tag="PKN91_02970"
     CDS             complement(27216..28139)
                     /gene="lpxC"
                     /locus_tag="PKN91_02970"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNS27064.1"
                     /translation="MVGQRTLKNAIRASGVGLHTGTKVLMTVRPAPPDHGIVFRRVDL
                     DPPVDIPACAENVGETTLGTVLVKGNARVSTIEHLMSAFAGLGIDNAIVEVNAPEVPI
                     MDGSAGPFVFLLQSAGIEEQAAPKRFVRILKHKRVEDGDKWAEFHPFDGFKVNFRIEF
                     DHPIFKRRTQSASMDFSTTSFLKEVSRARTFGFMRDLEAMRSHNLALGGNLDNAIVLD
                     DQGIMNEDGLRYEDEFVKHKILDAIGDLYLLGHSLIGEFSGYKSGHGLNNRLLRAVHA
                     DSEAWEEVVFESAKDAPINYVGTEALPSSAA"
     gene            complement(28259..>29162)
                     /gene="ftsZ"
                     /locus_tag="PKN91_02975"
     CDS             complement(28259..>29162)
                     /gene="ftsZ"
                     /locus_tag="PKN91_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNS27065.1"
                     /translation="IEGCDMLFITAGMGDGTGTGAAPVVAQVAKEMGILTVAVVTKPF
                     EMEGGKRMLVAEHGMAELGKYVDSLITIPNQKLLTVLGPQVTLLDAFKSANEVLQGAV
                     QGIAELITRPGLINVDFADVRTVMSETGMAMMGSGSASGEGRAREAAEAAVSSPLLED
                     INLQGAHGVLVNVTAGMDLSIGEFQEVGTIVKQYASEDATVVVGTVIDPDLASQLRVT
                     VVATGLGRIPVSTARPRDAGREPARAEPPMRVVRRSPLSSSDYAALDRPTVQRQRAVG
                     DGLRADIADEDVLDIPAFLRRQAD"
BASE COUNT         4672 a        10084 c         9588 g         4818 t
ORIGIN      
        1 actacctcac ctcactccac tcgtcagtag gttctgccgc ccagactcct aggcgccgcg
       61 cgcgtccgcc cgcagcacct cgaccaggcc gcgcatgtcc gccggcatcg gcgcctcgaa
      121 ctcgagcgcc tcgccgctca ctggctgcac gaacgcgagc cgcgcggcgt gcagcgcctg
      181 gcgcctgaag gcatgcagcg cctcgagcag cgcaggcgtg gcccctgccg tcagtcggcg
      241 gcgcacgccg tataccggat cgccgaggat cggataaccg atgtgcgcga ggtgcacgcg
      301 gatctggtgc gtgcgcccgg tctccagggt cacgcgcgcg agcgtatgcg agcggaagcg
      361 ctcgacgatg cggtagtgcg tgattgccgg gcggccatcg gcgcgcacgg ccatccgggt
      421 gcgcgcggcg cgatggcggc cgatgggctc atcgatcgtg cccccgccgg tcatcacgcc
      481 ggtcacgatc gcgaggtact cgcgcgagac ctcgcgcgcg gccagcatgg cgaccagcgc
      541 cgcgtgcgcc tcgatcgtgc gcgcgacgac catcaggccg ctggtgtcct tgtccagccg
      601 gtgcacgaga cccgcacgcg gcacctgcgc gagccgtggg tcgcgggcaa gcagcccgtt
      661 ctgcagggtg cggtcgcggt tgcctgcgcc ggggtgcacg acgaggcccg cgggcttgtc
      721 gatcacgagc acctgctcgt cctcgtagac gacctcgagg gccatggcct gcggcgcggt
      781 accggtatcg agcggcacct cgccctcgag gacgaccctt tccccgccca gcacgcggtc
      841 acgcgaacgc gccacccgcc cgtcgaccag caccgtgccg cgctcgatcc agccctggat
      901 gcgggagcgg gaatactgcg gcagtgcact cgccagcgcc tggtcgagcc gcattccggc
      961 cagcgtgggg gacaattgca ggtcgtggcg gcggaactcg gctgtcatta tttgtctata
     1021 ctgtcggttc cgaggccata ttccaacgag aggccccccg tcgatgtccg cactttacgc
     1081 gccgccccgc ggccgccttg tcctgttggc cgtactggcc tcgatcctcg ccgtctccgg
     1141 ctgccgcacc aacaagcagg acgactacgc gcgcaccaat ccggaagtgc tctaccagcg
     1201 tgcacaccgg agcatgaaga accaggactt caacaccgcg atcctcatgt acgaggcgct
     1261 gaccgcgcgc tatccgttcg cgcccgaatc gcgccaggca cggctcgacc tgatctacgc
     1321 ctattaccgg ggccgcgagg ccgagagcgc gatcgacgcc gccgagacct tcattcgcga
     1381 gaacccgacc catccgcgga tcgactacgc gtggtacatc aagggactgg tcgacttcga
     1441 gcgcacgccg aacaggctcg aacggatgtt caatgtcgac ctgtcggagc ggccgccgca
     1501 gacggcgggg cgcgccttcg cctcgttcaa gacggtcgtc gaacagttcc cgaagagcga
     1561 atacgcccac gacgcgcgcc ggcgcatggt gtacctgcgc aaccgcctcg ccgactacga
     1621 actctatgtc gcgcgctact acatgaagcg cggcgcctgg gtggccgccg cgcagcgcgc
     1681 caaacagacg atcgagcagt acgacggcgc accggccgtg cgcgaagcgc tcgaaatcat
     1741 ggtcgaggcc tacgaccgcc ttgaaatgaa agagctggcc gaacaggcgc gcgccgtcta
     1801 cgcggtgaac ttccccgcag agcagagccg cgtcgaagag aagaagtcct ggtggcgatt
     1861 ctggtagcgc ggccgcgccg cctgcctgct cagcgccgat atccgttcgt gatcggataa
     1921 cgccagtcgc ggccgaacgc gcgacggctc acccgtacgc cgggcggcgt ctggcggcgc
     1981 ttgtattcat tgcgtttgac catatcgagc acccgcccca ccgttgcccg atcgaagccg
     2041 cgcgcgcaga tctcgtcgac cgagaggtcc tcttcgatga acgccgcgag gatcgcgtcg
     2101 aggatctcgt acggcggcag cgcatcgcta tccttctgat cgtaccgcag ttcagccgag
     2161 ggcggccggt cgatcacccg ctgcggaatc gccgccgaca ggccgttgcg atattcggcc
     2221 aggcggtaga ccagtcgctt gctgcagtcc ttgatcggcg cgaaaccgcc cgccatatcc
     2281 ccgtacagcg tggcgtagcc caccgccatc tcgctcttgt tgccggtcgt gagcagcatg
     2341 cggccggtct tgttcgagat gcccatcagc agcagcatgc ggcagcggga ctggatgttc
     2401 tcctcggtgg catccggcgg gcggccggcg aactcgtcct tgagcgtggc gagtgtcgct
     2461 tcgaacatcg cctcgatcgg caacacgctg tacttcacgc cgagcaggct cgcctgtgcc
     2521 tgcgcgtcct caaggctcat cgatgaggtg tagcgcgagg gcatcatcac cgcgtgcacc
     2581 tggtccgcgc cgagcgcatc gacggctatg gcgagcgtta gtgccgaatc gacgccgccc
     2641 gacagaccga tcacgacacc cgggaagcga tgcttggtca cgtagtcgcg cacccccagc
     2701 accagcgccc cgtagacgct ctcgacttcg gacagctcgg gcgcgatcgc acccgccagt
     2761 ggcacgatgt cgccggcgcc ggtgcgcgtg aactcggcga aatacagacc ctcgacatag
     2821 gccggcgcac gcatcacgac ctcgccggcc tgattcatcg cgaacgactg gccgtcgaac
     2881 acgagttcgt cctgcccgcc gacgaggttc acatatgcca gcggcaggcc gacatcgcac
     2941 gccaccgacg ccgccacacg ctcgcgatcg cgctgcttgt gcaactggta gggcgaggca
     3001 ttgctgacga ccagcagttc ggcaccggcc gactgcgcga gctgcgccgc ctcgcgctcc
     3061 cagatatcct cgcagatgag gataccgacc ttcacgccgc cgacctcgac cacggtgggc
     3121 tgcgcgccgg ccttgaaata gcgcttctcg tcgaagacct ggtagttcgg cagctcgctc
     3181 ttgcgatgca tggcctgcac ggtgccgcgc atgaccagtg ccatcgaatt gtagatctgc
     3241 gcaccgctgt actcgggcag gccgaccagc acggcgacat cgcccacgcc cttgcacacc
     3301 tcggcgaggc cttcctcgac ctgccgccgc aggccgcgat gcagcagcag gtcctcgggg
     3361 ggatagcccg acagcgacag ctccgggaat accacgagat ccgcaccctg cacggccgct
     3421 tcgcgcgagg tttcgatcac gcgcgcgaca ttgccgcgca cgtcgccaac caggaaatcg
     3481 atttgcgcga gcgcgatccg cagcgatgcg gtgcgggtca tttcggcttg cgggcccgcc
     3541 gcttcgccgc tacgcgcttc ttcgccacca cgcgcttctt cgccaccggg cgcttcctcg
     3601 ccaccacgcg cttcttcgcc gccgggcgct tctgcgcgag cgcgcgtttc ccggccaccg
     3661 tacgcggctt cgccttggcc accgtgcgct tcgtcttcag gggcgggcgc ggcttgatct
     3721 cctgcgcctt ggcccgctgc ctgcgacgct tgaacagcag ttcgtgcatc gcactgccga
     3781 gatcggcggg ggacttcacg gtacgcacgc ccgcgcgctc gagtgcccgg tacttctccg
     3841 cggccgtgcc cttgccgccc gcgacgatgg cgcccgcatg acccatgcgc ttgccgggag
     3901 gcgcggtgac gcccgcgatg taggcgacca ccggcttcga gacgaaccgg cggatgaact
     3961 ccgcgccggc ctcctcgtca ctgccgccga tctcgcccac catgatgatg ccttcggtgc
     4021 ccgggtcgcg ttcgaacagc tcgagcacat cgacgaaatt catgccccgc accggatcgc
     4081 caccgatgcc gacgcaggtg ctctggccga gcccgttgtt ggtagtctgg aacacggtct
     4141 cgtaggtgag tgtgccggag cgcgacacga tgccgatcgc gcccttcttg tgcacgaagc
     4201 ccggcatgat gccgatcttg cattcgtcgg gcgtgatgac gcccgggcag ttggggccga
     4261 cgagccgggt accggtgccc gcgagcgcgg ccttcacctt gaccatgtca ttgaccggaa
     4321 tgccctcggt gatgcacacg acgagctcga tgcccgcgtc cgccgcctcg aggatcgcgt
     4381 ccgccgcgta cgggggcggc acgtagatca tgctcgccgt ggcgccggtc tgcctgaccg
     4441 ccgcatgcac ggtgtcgaac acgggcaatc cgatgtgctt ctcgccgcca cggccgggcg
     4501 tcacgccgcc cacgaggcgc gtgccgtagt cgaggcactg cttggaatga aaagtgccct
     4561 gcttgccggt gaagccctgg cagatgacgc gggtatgctt gttgacgagg atactcatgc
     4621 cttgaccttc ttgttcgagc cctgggcagc cagcgccacc accttcttcg cggcatcggt
     4681 gagatcggtg gccgggttga gtgcgaggcc gctgcccgcg agcatctcgc gcgccttgtc
     4741 ggcgttggtg ccctgcagcc gtacgaccac cggcacggcg acgccgagct tcttcgcggc
     4801 gatgatgatg ccctcggcga tcaggtcgca gcgcacgatg ccgccgaaga tgttgaccag
     4861 gatcgcgcgc acgttgggat tcgacaggat cagctggaac gcctcggtga cgcgctcggc
     4921 ggtcgcgccg ccgcccacat cgaggaaatt cgccgggcgc ccgccgtgca gtttgatcag
     4981 gtccatggtc gccatcgcga gtccggcgcc attcaccatg caggcgatgt cgccgtcgag
     5041 cgagacatag ttgagatcgt gctcgctcgc cttgacctcc atggcgtctt cctgcgtgat
     5101 gtcgcgcagc gccgcgaggt ccttctgccg gaagagcgca ttggggtcga tgttgatctt
     5161 ggcatcgagc gccaccagat tgccggccct ggtgacgatc agcgggttca cttcgagcag
     5221 gctggcgtcg cgctcgagat agagccggta gagcgcggtg gcgatctgct ggaactcgcc
     5281 gatctgccgg tcgctgaagc cgagcgcgaa ggccatctcg cggcactggt agggttgcag
     5341 gcccgcggcc ggattcacgt tcagcgtgac gatcttctcg ggcgtgtgct cggcgacttc
     5401 ttcgatgtcc atgccgccgg cggacgagcc gatcagcgcg atgcggccct tctcacgatt
     5461 cagggtgagc gacaggtaga tctcgcgtgc gatctccgaa cccgactcga cataaacggc
     5521 gttgatcggc aggccctctt cgcccgtctg cttggtgacg agccgttggc cgagcatgcc
     5581 ttgcgcggcc gcccgcaccg cctcgagatc gcgcgccaac ttgacgccgc cggccttgcc
     5641 cctgccgccc gcgtgcacct gcgccttgac gacccacacg gcgccgccga tcgccctggc
     5701 cgccgccacc gcctcgtcgg cgctgtgcgc cacgcgccct tcgggcacgg gcacaccgta
     5761 gctccgaaag acttctttcg cctggtattc gtgaagattc atgtgcccgc cgggtagaaa
     5821 aagaacgcct attctcgcgg aaagcggcac gctagagaag cggcggcgcg agaatgctaa
     5881 agtccgcggc gatgagggcg gatcgcagcg aagataattc gaccctgggc gacctcggct
     5941 ggcgggtcgt tggcctgctg aacctctatc ggctgctgat tccgccggtc ctgctcgcca
     6001 tccatcgctt caccgcgccg gcgccgaccg ttggcggcac ctttcccacg ctcttccacc
     6061 tgacctgcgc cgcctacttc gcggccgccg tggcgctggt gctcgcgcag cgcctgccgc
     6121 agatcccgcg gcgcgggctg atccttgcgc acgccatcgt cgatgcggtc gcgattggat
     6181 tgctgctcca ttcaagcggc ggcgtggcga gcgggctcgg catcctgctc gtcgtaccgg
     6241 tgggctcggt cgccctgctc gcagagggtc gccagaccct gctgatggca gccatcgccg
     6301 tcgtcgcgct gctcgcgcag caggtcatcg gccagatcgg cggcacggcc tcgccggccg
     6361 attacccctc ggcgggcgtg atcggcgtcg tgatcttcct cgtggccctc gcaagctggc
     6421 cgctcaccac gcggctgcgc gaaagcgagg ccctgatcaa gcggcgcgag ctcgacctcg
     6481 cgaatctcgc cgaactgtcg cagtacatcg tgcagcacct gcgcgagagc ctgctggtcg
     6541 tcgaccaggc ggactgcata cggctcatca acgagtcggc ggcgcgcatc ctcggcgatc
     6601 gcagcgcctt ccccggcgcc ctgctcggcg aggcctcacc gcgcctgctg tacctgctct
     6661 cgacctggcg ccagagcggc gaacgcccca aggacagcgg cgcgaccttc atcggcagcg
     6721 atggtgcgag cgagatcctg ccgcacttcg cgccgctcgg taaccaggac ccgccgccgg
     6781 tgctcgtgtt cctcgaggac acgacggaag tcgccagcaa gctgcagcag ctgaagctcg
     6841 cctcgctcgg gcggctctcg gcgagcatcg cgcacgagat caggaatccc gtcggtgcga
     6901 tgagccacgc ggcgcagctc ctcggcgaat cgccgcatct gtccgacgaa gaccgccgcc
     6961 tcgcggaaat catccgcgcc aatgccgcgc gcgtcagcga catcatcgcc aacgtgcaat
     7021 cgatgtcgag gcgcgaaccg ctgcggcccg aacagttcga gctcggtgcg tggctgcgcg
     7081 agttccgcac cgagttctgc tcgacgctgc cttgccccga ggatctggtc gagctgcacg
     7141 aatccggcga gggcctcgaa gtgcgctgcg atcgcggcca actgcggcag atcacctgga
     7201 acctggccga caatgcgatc aagcacaatc gtgcggtgcc tcacgcgatc gaactgcgtg
     7261 caggacgcct ctcgccctcg cagcgaccct tcctggaagt gtccgaccgt ggtggcggca
     7321 tacccgaggc gctgaccgag cgtatcttcg aacccttcgt gaccagcgag gcgcgcggca
     7381 cggggctcgg gctgttcctc gcccgcgaac tcgcgcaggc caacggcgcc acgctgatct
     7441 atgaaccacg tcccggtggc ggcagcgtgt tccgcctggt gttttccgat ccgctacgat
     7501 ggcgggcatg agtcgacctg ccgtgctggt ggtggacgac gagccggatc tcgtcgaact
     7561 ggtctgcctc accttgaacc gcatggacct cgaaaccgcg tccgccggcg acgtggccgg
     7621 ggcgcgcgag ctgctgaaca cgcggcactt cgacctgtgc ctgaccgaca tgcgcctgcc
     7681 cgatggcgac ggtctcgacc tcgtcgcctg gatccaggag aacaagccgc tcgtgccggt
     7741 cgcggtgatc tcggcgcacg gcaatgtcga atcggcggtg cgcgcgctca agctcggcgc
     7801 attcgacttc gtctcgaagc cgctcgatct cggctcgctg cgcaagctgg tggcgagcgc
     7861 gatcaagctc ggcgagcgcg ccgacggcga aatgacgatg cgcgggccgc gactgatcgg
     7921 ccattccgcc gccatggaca cgctgcgaca gatgatcgcg cgcgtggcgc gcagccaggc
     7981 gccggtgcac atctcaggcg agtcgggcac cggcaaggaa ctcgtcgcga gactgatcca
     8041 cgaatccggc cctcgtgccg aggggccatt cgtgccggtg aactgtggcg cgatcccgac
     8101 cgaactgatg gaaagcgaac tgttcggcta caagcgcggc agcttcacgg gcgcggtggc
     8161 cgacagcaag ggcatgatcc agtccgccga aggcggcacg ctgttcctcg acgaggtcgc
     8221 ggacctgccg ctgcacatgc aggtgaagct gctgcgggtg atccaggaga aatccgtgcg
     8281 cccgatcggc gagacccgcg aagtgccggt cgacgtgcgc atcctgtcag cgacgcacaa
     8341 gaacctcgcc gcgctggtgc gcgatggccg cttccgcgaa gacctgtact accgcgtcaa
     8401 tgtgatcgag atccgcgtgc ccgcgctgcg cgagcgcccc gaggacatcc cggacctggc
     8461 cgcgaccatc ctccggcggc tgtcgcgccg catgggagtc gaagcccccg acatcagtgc
     8521 cggggcgctc gccgtgcttg ccgcgcacag ctttcccggc aacgtgcgcg aactggagaa
     8581 cgtgctcgag cgcgcgctga cgctctgcat taccgacacg atcgaagcgg gtgacatcca
     8641 gctgcgcgcg ccgatgctcg gcgatccggc gccggccacg atgtccgccg gcggcagcgc
     8701 ccagcaggag gcagccgcga cgatcggggg cggcgcgctc ggtgagcagc tcgacagcat
     8761 cgagcgcgac gcaatcgtca aggcactcga gcagacccgc tacaacaaga ccgcggccgc
     8821 gaagctgctc ggcatgagct ttcgcgcgct gcgctaccga atcaagaagc tcgggatcga
     8881 ataggccggt gcgcatgccc cgcggattcg cgaggtgcat cacatgaatc gacatcgttg
     8941 cttggtcgcg caggcgagtc gctccagaat tcttgcatga agccgcaatg gattgccctg
     9001 gcgatgatct gggtcacgtc ggcgccggca cagccgacgc cgaacgtgca gatcgagctg
     9061 gccgaaatcg accccgcggg cgtcccgacc gtggcgcccc acacgccggt ctacggccgc
     9121 atcgtctaca cgaccgatgc cccggtgcgc ctcgtcctgc tgccgtaccg caatggccag
     9181 cccgtgctgg atggcctgta caattccggc tcgccccggt acgcgccggg cagtggcgag
     9241 accatcgcct ggttcgcctt cgacgaaccg cagaccatcg acgagatccg cgccgtcgcc
     9301 aatgatttct acggcaacgt cttcgccgag gactcgatcg tgcggaacct gaactggcgc
     9361 gagggcgcgc cgaagccgca gaaggccgcg tgggtggagc cgctcaagag agccgggaag
     9421 gacgtgatcg tcgagccgcc cgccgaatat ggctggttcg cgaccgtcgt cacgcagttg
     9481 ttcttcctcc tcgtgccgct gtctgtgggc ctgcagatcc atgcgtggcg caagctcgac
     9541 ggcggccccg ggcggctcgc ccgcctgtcg gggtatgcga tgggggcgct gtggctgttc
     9601 gtcatcctga ccggggtcgc gggcagcaac ctctcgccga tctggatcgt gttcctgagc
     9661 ccgctgttcg tactgtttct cggcgcgttg ctcctcgtcg cacgcacccg acgccgccga
     9721 ttcgcctgac ccgcaagtct cggcaggtca ctgtgacgca gttctcaggt ggcgccacca
     9781 aagcgaaagc tggcacacac gaaggcagtg accacttgtg tcacttcctg acacgaagag
     9841 cagcacagcg tcgcgccgcg ctgacaaggc gcgtgcgaca tagctgcgtc gcgtgtcggc
     9901 gaacactgcc aattcagcaa gttagataca catcacattg ctggcacgcc cgttgcttca
     9961 tgctttccct gcaggcgtac agcgccgcga atttggttaa cggctggaag atttctggac
    10021 tcaaggagct tattcacatg aagaccatgc agaagggttt taccctcatc gaactgatga
    10081 tcgtcgttgc gatcatcggc attctcgccg cgatcgcgat cccggcgtat caggactaca
    10141 cgatccgctc gcaggtcact gaaggcctga acctcgcgtc ggccgtcaag gccggtgtcg
    10201 cggagacctt tgcccagacc ggcgaatggc ccgctgacat ggacgcggcc ggcggtgacg
    10261 gcgcgaatct gccgagcggc aagtacgtgt cgagcatcga cgtcagcaac ggcacgatca
    10321 tcattaccta cggtaacgat gccaacgccc agctcgtcgc cacgccgact ctgacgttgc
    10381 agccgcgtat cagcgccaac aacgacgttg tgtggctgtg cggctactac accggcgccg
    10441 acgttgccgg cctgaccgac ccggtctcgg gcgcgtccga cgcgggctcg accgacgtca
    10501 cgccgaagtt cctgccgcag tcctgccgcg acgatacctg atcgtcggcc tggcttgtct
    10561 gcacgaagcc cctcccccgg aggggcttct ttttttggga ggggagtcac gcagcctccc
    10621 tgccctctgt gcgaccatcg gcagagattt gacaattgcc cgggggtcgc cctccggtac
    10681 actatcgcct tgacgggtcg ctgactgctt gctgcaagtg catgaacgat aacgcttctt
    10741 tggccatcgg cggcgctcgc tccgccctcg gcgggctgcc ccagcggctc gtgcaggacg
    10801 ggctcgtcga cgaggcgacc atgcacagcg cgctgcaggc gtccagggag cgcaagtccg
    10861 gtgtcgtgac ccagctcgtg ggcatgggcg ccgccaaggc gcgcgacatc gcggtggccg
    10921 cctcgcacga attcggcgta ccgctgctcg acctcgactg cctcgctgtc gacctcgacc
    10981 tcgtgcgcct catcagcgac aagctgctgg gcaagcaccg ggtgatgccg ctcttccggc
    11041 gcggcaagcg tctgttcctc gcggttgccg atccgaccaa cctgcaggcc atcgacgaaa
    11101 tcaagttcgc gaccgggctc gggatcgagg cgatcgtcgt cgaagacgac aagcttcagc
    11161 gcgccgtcga caaggcgctc gagcaggtcg acacccagat gccgaacctc accgacgagg
    11221 aaggcgtcga tctcgagggt ctcgaggtca cgggcggcga agacgaaaac gacggcggca
    11281 tcacccgcga cgacatcgac gacgccccca tcgtcaagtt cgtcaacaag gtgatgctcg
    11341 acgcgatccg ccgcggtgcg tccgacatcc acttcgagcc ttacgagaag atgtaccgcg
    11401 tgcgctaccg catggacggt gtgctgaagg aagtggcgca gccgccggtg cagctggcgc
    11461 agaagatcag cgcgcgcctg aaggtcatgg cgcgcctcga cattgccgag cgccgcgtgc
    11521 cgcaggacgg ccgcatcaag atgaagctgt cgaagacccg cgcgatcgac ttccgtgtca
    11581 gcacctgccc gaccctgtac ggcgagaaga tcgtcacgcg tatcctcgac ccgtcgcagg
    11641 cgatgctcgg catcgatgcg ctcggctacg aaccgcagca gaaagagctg tacctcgcga
    11701 acctcgccaa gccccagggc atgatcctcg tgaccggccc gaccggcagc ggcaagaccg
    11761 tttcgctcta taccgggctc aatatcctca atcgcgagga cacaaacatt tcgaccgccg
    11821 aggacccggc ggaaatcaac ctgccgggcg tcaaccaggt caacgtgaac cccaaggtcg
    11881 ggctcacgtt cgcctcggcg ctgcgcgcct tcctgcgcca ggatcccgac gtggtgatgg
    11941 tcggcgagat ccgcgacctg gagaccgccg aaatcgcgat caaggccgcg cagacgggcc
    12001 acctcgtgct ctcgactctg cacacgaacg atgcaccgca gaccctcacg cgactcatcg
    12061 acatgggcgt caagccctac gcgatcgcga cctcgtgcag cctgatcatc gcgcagcgcc
    12121 tcgcgcggcg cctgtgcacg caatgcaagc atctcgtcga cgtgccggcc gaggcactgc
    12181 tcaaggaagg cttctcgaag gaagacgtgg cggccggcat caaggtctac ggcccgaagg
    12241 gctgccaggc ctgcaccgac ggctacaagg gccgggtcgg catctaccag gtgctgccgg
    12301 tcaccgagga gatcgggcgc atcatcatgg aaggcggcaa cgcgatccag atcggcgacg
    12361 cggcgaagcg catgggcgtg tgggacctgc gccgctccgg cctcgagaaa gttaaacagg
    12421 gcatgacgag cctcgaagag atcaacagcg tcaccatcga gtgacctgac ggagactggg
    12481 catggcgaac atcgcagcaa ccgcaagcgt ccgcacgatc aagcgggaca tcccgttctc
    12541 gtgggagggc atggacaagc gcggcaaccg ggtcaagggc aagagcctgg cgccggacga
    12601 ggccgcgctg cgtgcggacc tgcgccgcca gggcatcgcg ccctccaagg tcaagaagca
    12661 atcgcaggcc ttcaagagcg gtggcagggt caagccggaa gacatcgcgg tgttcagccg
    12721 ccagcttgcg accatgctcg cctcgggcat tccgctcgtg caggcattcg agatcgtcgg
    12781 caacggccac gagaagccct cggtgcagaa gctgatcctc gacatcaagc aggacatcga
    12841 gggcggtacc tcgctgcatg aggcgctcgc caagcatccg ctgtacttcg acgatctcta
    12901 tgtgaacctc gtcgaggccg gcgagcaggc gggtgcgctc gagaacctgc tcgacaagat
    12961 cgcgacctac aaggaaaaga ccgaggcgat caagaagaag gtgaagaagg cgctgtttta
    13021 cccggcggcg gtcatggtcg tggcggtggt cgtcacgctg atcctgctga tcttcgtgat
    13081 tccccagttc gagagcctgt tcaagggctt cggcgccgag ttgccggcat tcacgcagat
    13141 ggtcatcaat ctgtcgaatt tcgtccaggc gaatgggatc tacctggccg tcgtcttcgg
    13201 cgcggcgggc tatgccttct tctacttcaa gaagcgctcg aggaagatgc gcgaggtgct
    13261 cgaccgcatt tcgctcaagc tgcccatcat cgggccgatt ctcgtcaagt ccgccatcgc
    13321 gcgctatgcg cgcacgctgt cgaccatgtt cgccgcgggc gtgccgctgg tcgaggcgct
    13381 cgaatcggtg gccggcgcca ccggcaacgt cgtctacgag aatgccgtga tgaagatgcg
    13441 cgacgaagtc gcgaccggcc agcgcctgca gcgcgcgatg gagaacacca gcctgttccc
    13501 caacatggtg atccagatgg tggcggtcgg tgaggaagcc ggcgcgctcg atcagatgac
    13561 cgccaaggtg gccgagttct acgaggccga ggtcgacaac gcggtcgaca gcatgtcgag
    13621 cctgctcgag ccgctgatca tggcgatcct cggcgtgctg gttggcggtc tcgtgatcgc
    13681 gatgtacctg ccgatcttca agctcggcgc ggtcgtgtga tcgcagtctg cttcgttctg
    13741 gggctgctgg tcggcagctt cctgaacgtg gtgatccacc gcacgccgct cattctcgag
    13801 cggcagtggc ggcgccagtg cgacgagctc gcgggccgcg agacgcccac ggagccgcgc
    13861 tacaacctcg tggtgccacg ctcggcctgc ccggcctgca aggccccgat caaggcgatc
    13921 cagaacgttc cggtgctcag ctggctcgcg cttcgcggcc gctgcgcgag ctgcaaggcg
    13981 aggatcagcg cgcgctatcc gctggtggaa ctgctcacgg cgatcctgtc ggcgggcatc
    14041 gcgtggcggt tcgcaccggc cggcctcccg gttgtgggcg ccgcgctcgt gctcacctgg
    14101 tttctgatcg cgctcacctt catcgacttc gacacgcagc tgttgcccga ctcgctcacc
    14161 ctgccgctgc tctggctcgg cctcttcgcc agtgtaatgg gctatacggg catcgcgccg
    14221 cgcgatgcca tcatcggtgc gctggcgggc tacctcgcgc tgtggtcggt ctatcacctc
    14281 ttccgcctgg ccaccggcaa ggagggcatg ggctacggcg acttcaagct gctcgccgcg
    14341 ctcggcgcct ggctcggctg gcagatgctg ctgccgatca tcctgctgtc ggcgctcgtc
    14401 ggtgcgctga ccggcatcac gctgatcgtc gtgcgcggcc gcgaccgcag tgtgccgatc
    14461 ccgttcggcc ccttcctcgc ggccgcgggc tggattgcgc tgatctgggg gccgcagatc
    14521 gtggcgggct acctgggaag aatgtgagcg gcgtgtttcg cgtcggcctc accggcggca
    14581 tcgccagcgg caagtccacc gtcgcgaatc ttttcgccgc gctcggcgtg ccggtcatcg
    14641 acaccgacca gatcgcgcgc gacgtggttg cacccggcac accgctgctc gcgaccctgc
    14701 gcgagacttt cggcgaggac gtcatcgccc ccgacggcac gctcgatcgc aaagccatgc
    14761 gcgcgcgcgt cttcggcgac tcgcaggagg cccgggcggc gcggcgcacg ctgaacgaac
    14821 tgacccatcc tgcgatcctc gcggaaatgg aagcgcgatc gcagcaggca ggcggtcctt
    14881 accagatcct tgcgattccg ctgctggtcg aggggcaatc gcgctcgcgg gtcgaccgca
    14941 tcctggtcgt cgacgcggat gaggccaccc ggatccgccg gctgcaggcg cgcgacgggt
    15001 ccacgctggc cgaggcgcgc gccctgctcg ccgcacaggc cgatcgtgcc acgcgcctgt
    15061 cggccgccga cgacgtcatc gagaatgacg gcgatatcgc ggcgctggcg gcgcaggtcg
    15121 agcgcctgca cgcggcctat ctcgccgccg caggcgttta cgggagcgga caacgtcaag
    15181 cacaatagcc ggatgtccga ggacccggcc atccgccagg cctccttgcc cgaggaggcc
    15241 gagctcgttt tcgagcagcc gctcaatgag cgcatgcgca cattcctgcg catcgacttc
    15301 ctctacggcc aggccactta ccacaacgag catgcgagcg agtggagcag ccgcgccgcc
    15361 gtcgccagcc tcatggacat cctcgcgatc acggcccgca ccgatgtgcg cgccgatgcg
    15421 ctgaaggagc tcgagcgcca gctcgcgatg ctgcacgagt tccagacgag gcccaatgtc
    15481 gacgggtcgc ggctgcgcac cgtcatgtcg aacctgatgc ggctgcgctc cgagctgctc
    15541 gggaccggca ccggttacct gcagccgctg aaggaatccg agttcctcgc cgcgatcagg
    15601 caccgcagcg cgatcccggg cggtacctgc gaattcgacc tgcccgacta ctactactgg
    15661 ctgcagcaac cggcggagcc gcgcgcgcag gccttcaacg agtggctcgc gctgctgcgg
    15721 ccgctgtgcg attcgatcgc ggagctgctg tggctcacgc gccagaacgg ccggccccgg
    15781 gaggaaaccg ccaagggcgg tgtcttccac atcacctttg accgcgactc gccggtgcag
    15841 ctgctgcgca tcagcgtgcc gcaatcgctc gggctctacc ccgagatcag cggcagccac
    15901 tatcgttcga gcgtgcgctt cctcacctgg aatggcctcg gcgcccgccc gagccaggcc
    15961 accatcgacg tgccgttcac actgagctgc tgcaattgaa ggtcgcctgc cccacctgca
    16021 agcgcgagat cgaatggaac gccgaggcgc cctggcggcc attctgcagc gaccgctgcc
    16081 ggctcatcga cctcggcgca tggctcagcg aagagcatgc gattccgggg gaaccggcgc
    16141 cggaagccga cccggcgcca ggctcccgcg actaggctca gcgcgccggc gtcggcatgc
    16201 cgagcgcctt cgccgcaaac gcccactggt ccgcgaagtc ctcgatctgc atccataccg
    16261 gcttgccggc accgtgcccg gcacgggtct cgatgcggat caggaccgga ttgtcgcagc
    16321 cctgcgccgc ctgcagcgcc gcggcgaact tgtaactgtg ccagggcacc acgcgatcgt
    16381 cacggtcggc ggtcgtcacg agggtcggcg gatagcaggc gcccttcctg acattgtgca
    16441 ccggtgaata cgcgagcagc gccttgaact gctccgcatc ctcgctgagg ccataatcac
    16501 ccgaccactg gcgcgcgttg gcgctcgcag tgtgatagcg cagcatgtcc agcaccccga
    16561 cgccgggcag cgccgcaccg aacagctcgg gccgctgcag catcaccgcg ccgacgagca
    16621 gcccgccatt gctgcggccg ctgatcgcga tgcgcgaggg ctgcgtgtat ttctccctca
    16681 ccaggtactc ggccgcggcg atgaagtcgt cgaacacgtt ctgcttgcgc gtgagggtgc
    16741 cggcgtcgtg ccaggcctcg ccgtactcgc cgccgccgcg caggttcgcc tcggcataga
    16801 cgccgcccat ctcgagccat gcgagcacgg tcggccggaa caccggagtc agcgagatat
    16861 tgaagccgcc gtaaccgtac agcagtaccg gctggttgcc gtcgcgcggc atgtcccgcc
    16921 gatgggtgat gaacatcggt acccgcgtgc cgtccttgct cgcatagaac acctgctcgg
    16981 tgacgaaggc gccggtatcc gcgtccacct tcggtgtgcg gaaagcggtg accccgttgg
    17041 tcgcggtgtc gagacgcagg atgcgtgtag gggcgaggta gtcggagaag gcgaagaacg
    17101 cctccgcatc gccatccttc gcttcgaagt taccggcaga ccccgggccc ggcaacggca
    17161 cgtcacccac aggcttgccg ctgcgctcga agagccgcgt gacgttgcgt gcgtccttca
    17221 cgtagttgac gaccagccgc tcgccggcga tcgcgaccgc gtctatggct tcgcgcgcct
    17281 ccgggatgat ggtgcgggcg cccgcggcac tcggggcggc gcgatcgacc gcgagcaccc
    17341 gccagttcgg cgcgccggag gaggtcacga aataaagcgt cgtggcgtcg tcgccggcgt
    17401 agaagtagtt ggcgtcgaag tcggtgaaca gcttgacgac cgcgccgtcc ggtcgggtga
    17461 ggtccttcag cacgacgcca tcgcgtcgcg tgtcctcgta gatcgaaatg accagcagct
    17521 tgccgtcgtc ggtcacgtcg gcggacggcg cgcgggtcgg gtgatcggtg accgcgtaga
    17581 cgagcgcatc gcgctcctgg ggctcgccga gcctgtggaa atagaccgcc ggtcggccgg
    17641 catcgtcgcc cttgccgtcg gcgcgcgccg gatagcggct gtagtaaagg cccgacccat
    17701 cgggcgccca gctcatctgc cagaacttca cgcgacgcag ctcgtcgggc agatcgacac
    17761 cgtcgtcgac gcggcggaac ttccagatct cccagtcggt gccaccgtcc gacagcgaat
    17821 aggcgagcag cttgccatcg gggctcggct cgaaacgcgc gagcgctatg gtcgcatcct
    17881 cgctcgcgat gttcgggtcg aacagcacgc gaccctcgcc cgaggccgag tcgctcacgt
    17941 acagcacgtt ctggttctgg gtgccgtcgt tgcgcgtgta gaaatagcgc ccgcccgcct
    18001 ttttcggcac gccgtagcgt tcgtagttcc acagcttcgt cagccggtcc ttgacccagg
    18061 cacgctgcgg gatagcctcg agccacggct gggccagcgc gttctgcgcc gtaacccact
    18121 tcttcgtggc gcgggaatcc atgtcctcga agccacggta gggatccgcc actctggtgc
    18181 cgaaatagtc atcgaccgcg gacccgcgcg gcgaaccggg gtaacgcagg ccggcggcgg
    18241 ccagtgccgc ggcgccgagc gtgatcgtca tgacaatgag caacgggagc gcgccgcggc
    18301 ggcgcgtcga ttggctgtca ttcatgttct tgcctaaact cgcgaattgc cgatgcggca
    18361 gtctaacaaa gtgatccgcc ccatgaccgc actgcgcccc actcgcttcg tcgcattgct
    18421 cctttggctg gtcgcctggc cggcggcggc cgacgaactg acgatcgagc gactgttctc
    18481 cgcgcccgac ctgtcgggcc ccacgctgcg cggtgccact ttcgcgcctg atggcgagcg
    18541 catcgcctac ctgcgcgcca aggcggacgc gaaggacacc ttcgacctgt gggcctacga
    18601 gatccgcacg cggcgccacg tgctgttggt cgattcagcg cggctcgcca cggcgggcgg
    18661 cgcactgtcc gccgaggagg aagcgcgccg cgagcgccag cgcacctcct cgctcggcgg
    18721 catactcgaa tacgacctgt cggccgatgg ccggcgtatc ctggtgccgc tcggcggcga
    18781 cctgtacgtc tacgatctcg gtgcgccagc cgagcgcgcc gtgcagcgca tcacgcagac
    18841 ggcggcctac gaaaccgacg cgcgcttctc cccgcgcggc cgctacgtga gcttcatccg
    18901 cgagcagaat ctgcacgtgc tggaactcgc gagcgggcgc gaaatcgcga tcacgcaggg
    18961 cggtggcggg ctcgtctcct acggcgtcgc cgagttcatc gcccaggagg agatgaaccg
    19021 caataccggc tactggtggg cgccggacga atcgcgcatc gcttacgcgc gcgttgacga
    19081 ggcgccggtc gccgagatcg agcgcttcga gatccatgcc gacagcgtga aggtcgtgaa
    19141 gcagcgctat ccggccaccg gggagcgcaa cgccgacgtg cagctgttcg tcacgcacat
    19201 cgagggaggt ccggcggcac gcgtcgacct cggtgcgaac cccgacattt acctggcgcg
    19261 agtggcctgg ttccccgatg cgcgatcgat cgcgatacag cgccagagcc gcgaccagaa
    19321 attgctcacg ctgctgaagg ccgatgccgc gagcggcgcg acccgcgccc tgctcgagga
    19381 gcgctcggac acctgggtcg atatcgtcga cgacttcgtg ttcgtcggtg accgcgggga
    19441 tttcctgtgg ggctcgtcgc gctcgggcta ccaccacctg tacctgtacg gggccgatgg
    19501 cacgctgaag cacgcagtga ccggtggcga gtggatggtg accgccgagg accgcggcga
    19561 ctcgttgcgc gcgatccgtg gcgtcgactc gcgcgccggc ctggtttact tcatggcgaa
    19621 catggcctcg ccgatcgagc gccagctcta cgtgacttcg taccgcaagc ccggcgagcc
    19681 gcgccggatt tcacgcgagg cgggctggca caccgtgcgc atgtcgcccg acacgcgcta
    19741 ctacctcgat acctggtcga ccagcgagca gccgccgtcg gtggcgctgc gcaaactcga
    19801 cggcagcgtg cgcgaggccc tcgtcgcgaa cacgctcgat gccacgcacc cctatgcgcc
    19861 ctacctcgat gcgcacaatc cctcgacctt cggcacgctg cgggcgagcg acgggcagac
    19921 cctgtactac cagctcaccg caccgaagca cctcgtgccc ggcaagcgct atccggtgat
    19981 cgtcgacgtc tatggcgggc cgggcgtcct cgggcgcgta cgcaacgcct gggggaacct
    20041 cttccgccag tatctcgtac agtcgggcta cgtcctgttc acgctcgaca atcgcggcac
    20101 cgcgatgcgt ggcgtgcagt tcgacaccgc ggcctttcat cgcctcggct cgatcgaggt
    20161 cgaggaccag gtgaaggggg tcgagttcct gcgctcactg ccgtacgtgg atcccgcgcg
    20221 catcgggatc tggggctgga gttacggcgg ctacatggcg ctgatgagca tgatgcaggc
    20281 gccgacccac tttgccgcgg gcgtcgccgg cgcgccagtc accgatttcc gcctctacga
    20341 cacgcactac accgagcgct acatgggcac gccggcagcg aacccggcgg gctacgcggc
    20401 gagcaacgtg ctggcgcacg ccgaaggcct cgccggcccc ctgctcgtga tccacggcat
    20461 ggccgacgac aacgtgctgt tcacccacag caccgcgctc ttcaagcgcc tgcaggatct
    20521 cggcaagcca ttcgacgtga tgccctatcc cggtggcaag catggcctga tcaggcacgc
    20581 ggacatgggc ccgcacgcct accagagcgt gaagcggttc ttcgacgaga acctgtaggg
    20641 cgccggcgcc tcagggcagc ttgagcttgc cgcccggacc gatgggcggc agcccgcccg
    20701 tctcgggcac gacgatgcgc gaggccgcct cgcggcgata ctcgcgtgcc tcgtcgatcg
    20761 cctgctcgag cgccgtcttg accgcatcgg ggaacttcgc gagcgcctcg gacagcgtct
    20821 tcgcgtcgat ctcgaacatc agcgggagca cgccacccgg cgtcagcagc tgcgtctggc
    20881 ccgagtacag cacctcgcgc gtggcgtcat cgctgccatc gaccgtgatc ggcaccagcc
    20941 gccgcagcgt gccggccttg cggtcggtga acacttcctc gcgatagagg ctggttgcat
    21001 cgagctggat gtcgggcaga ttggggtcgt tagccatggc gtgcctgtgt gtcgaggggt
    21061 ggaattgttt cgcgagtggt cagcgggccg gcgccgcgcc ctgctcggcg agcacggcga
    21121 cggtagcggg cgtgccggtc tggcgcagca gggccttggt gcggaagaaa cgcatgtacc
    21181 ccgccggtcc catgcgcgag ccgccgaggc ccgacagctt gaaagaattc ttttccgcct
    21241 cgtgcatcat ggcggtgagc gagcagtcgt tgaggctcac ggcgccggcc tcgatccggt
    21301 gggcgatcgc ttccgcctcg gcaagcgagc cggccagcac ggcggcggac agcccgtaga
    21361 tgctgtcatt ggcgagcgcg atcgcttcct cgagcgtgtc ataggccatc accgggatca
    21421 cgggaccgaa ggtctcctcg gtcatgacct gcatgtcatg ggtcacgtcg acgagcacgg
    21481 tcgggcgcag ccattcgccg ccgtggttct cgatctcgcc gcccgcgagc accctggcgc
    21541 ccttggcgag cgcatccctg atctgcgctt cgacgacgcg cgcctgcggc gcgaagatga
    21601 acgggccgac gtagccgctg tggatgtcag gccagttgat gtcgatggca cgcgccttgt
    21661 cggcgagcgc ggccacgaac gcatcgtgga tccgccggtc gatgtacacg cgctcgagcg
    21721 actggcaggc ctggccgctc gcggtgatcg ccgcgcgcag cacgatgtcg gcggcacgct
    21781 cgggatcgaa gtccgcggtg atgatcacgg gatccttgcc gccgagttcg agaaaggcgg
    21841 gaatgaaatg ctgcgcggcg ttcacgccga cgagccggcc cgtcttgacg ctacccgtga
    21901 aacacacggc atccacgttg gcaaccagcg cctcgcctgt ctcgcgccct ccgggccgca
    21961 cggcgagcac cgcggccaac tccggcacgg catcgatcgt gcgctgcacg ggggccaccc
    22021 agcggggcgt gacctcgctc ggcttcacga gcacggcgca accggcgagc aacgccggga
    22081 tcgcatcgat gaaggcgagg atcagcggga aattccacgg gctgatcgcc ccgaccagcg
    22141 gatacggcac gagtgtgtag ccgtaggcga cgccgggcca ggcctccgag cgcccgtcgg
    22201 gttgcgcgcc gagcgtgggc gccaggcgac accagcgatc gatcgcgccg agcatcgagc
    22261 cgaactcgcc cacggcgatc acgtgacgcc cggtgtcctg cgacagggcc gcaacgatct
    22321 cctccttgtg cgactcgagg gtggccttcc agcgctgcat gacggccacg cgatgcgcga
    22381 taccggcatc acgccacacc ggctgctgat gacgcagcgc cgcggcaatg cggccgattt
    22441 ccgcggtcgt cggcgcggtg aagtggaagt cgacctcacc gctgcgggga ttgcggacgt
    22501 cgaaattctg gtctgtgctc atgtcggtcc cggtcagcgc gccaagggcg cgaacgaaag
    22561 gctcatcggc ctccagaata tcccaatcgc cgagctcgcc cggcgcggcc caggcgattt
    22621 gttgcccatc gagcggacgc gggtccccgc gccagtcctc gacgagccac agcgacagct
    22681 cgacatcgcg atcagggtag cggtgcgccg aagtcatgaa cggccgcgcc gcgatcacct
    22741 tgatgccgag ctcttcggtg agctcgcgct cgagtgcggc acgctcgtcc tcctgagccc
    22801 cgaccttgcc gccgggaaac tcccagcgtc ccgccatgtg cttgcccggc gggcgatcgg
    22861 cgatcagcac gcggccgcgt gcatcgaaca gtgccgctgc gaccacgcgc accggcaggc
    22921 gtgtcaccgc gaagcgggtc actccgcctg cgccagcgcg ccgtggcagt gcttgtactt
    22981 cttgcccgag ccgcacgggc acggctcgtt gcgaccgacc ttgcgctcgg tgcgcaccat
    23041 cggcgtatgc gactcggccg ccggcgcggc ggcacgggcc ggcaggcgcg tgacaccgcc
    23101 ggctggcgcc cctgcacccg cagcgcgcgc gccgcccggg gccgccgcag cctcgcccgc
    23161 ggcctcgccc gccgcgagtg cggactgcgc atcctggtgc tgggcctgca gcgcgcgcat
    23221 cagtcgctgg cggcgctgct cctcctcgcg gtcgatctcg tcctgcgtgc gcacctcgat
    23281 cttcgcgagg atggacgccg tctcgaactt cacgcgctcg agcatcgccg agaacagctc
    23341 gaaggcctcg cgcttgtact cgtagcgata gtctttctgc gcatagccgc gcaggtggat
    23401 gccctggcgc aggtagtcca tggcggcgag gtggtcgcgc cagtgctggt ccagggtgcg
    23461 cagcatcacg tccttctcga cgtggcgcat cagctcgacg ccgatgcggg cgaccttctg
    23521 ctcgtatgcc gcctcgaccg cctcgacgat gcgcgcgcgg aacgtctcct cgtcgatctc
    23581 cggttcggcc tgcacccact cctgcggcgc gatcctcgtg ttgaagtcct tcagcaccga
    23641 ctcggcgagc ccctggagat cccagtcgga cggcccggcg cccttcggca cgaactggtc
    23701 gaccatcgtg ccgagcgcat cggcgcggat gccacgcacc gcatcggcga tgtcgtcctt
    23761 cgccatgatc tcggtgcgtt gctggtagat gaccttgcgc tggtcgttcg cgacatcgtc
    23821 gaacagcagc agctgtttcc tgatgtcgaa gttgtgcgac tcgaccttgc gctgcgcctt
    23881 ctcgatgcgg cccgagagca tgcggctctc gatcacctca ccctgcttca tgccggccat
    23941 cgtcaacagg cgcttggtga agttcggatc accgaagatc cgcatcaggc tgtcttccat
    24001 cgacaggtag aagcggctcg aacccggatc gccctggcgg cccgagcggc cgcgcagctg
    24061 gttgtcgatg cgccgcgact cgtggcgctc ggtgccgatg atgtgcaggc cacccgcggc
    24121 gaccacgcgg tcgtggcgcg cctgccactc cgcgcgcacg cgctggcggt ccgcgtcgga
    24181 ggcttccggc ggcaatgcgg caagctcggc ctcgaggttg ccgccgagaa cgatgtcggt
    24241 gccgcggccc gccatgttgg tcgcgatcgt ggcggctccc ggccggccgg cctgcgcgac
    24301 gatgtgtgcc tcgcgctcgt gctgcttggc attcagcacc tggtgggtga tgccttcctt
    24361 ctgcagcagc gcggaaatct cctccgaggt ctcgatcgag gtcgtgccga cgagcaccgg
    24421 ctgctcgcgc tgcgcgcagt cgcggatgtc ctcgatgatc gccttgaatt tgtcgtcctt
    24481 ggtcagatag acgagatcgg cgtggtcctt cctgaccatc ggccggtggg tcgggatcac
    24541 caccacttcg aggccgtaga tctgcaggaa ctcgggcgcc tcggtatcgg cggtgccggt
    24601 catgcccgag agcttcctgt agatgcggaa gtagttctgg aaggtgatcg aggcgaccgt
    24661 ctggttctcc tcgcgcacgc gcacgccttc cttggcctcg atcgcctggt gcaggccgtc
    24721 ggaccagcgc cggccctgca tcgtgcgacc cgtgaactcg tcgacgatga tcacctcgcc
    24781 gccgcgcacg atgtactcga cgtcgcgacg gtagatcgcg tgcgcgcgca gcgccgcgtt
    24841 cagatggtgc atcaggcgga tgttgccgga atcgtagagg ctctcgccct cgcgcagcag
    24901 ccccccctcg agcatcaggc gctcgacgtg ctcgtggcct tcctcggtga tgtgcacctg
    24961 cttctgcttg aggtcgatcg agaaatcgcc ggggccgttc tcctcctgct ggggcctgag
    25021 ccgcggtacc agcgagttga cccgcaggta catctcggtg ctctcctcgg cggggccgga
    25081 gatgatgagc ggcgtgcgcg cctcgtcgat caggatcgag tcgacttcgt cgaccaccgc
    25141 gaaggcgtgt ccgcgctgca cgcgatcctc gagccggaac gcgaggttgt cgcgcaggta
    25201 atcgaagccg aattcgttgt tggtgccgta ggtgatgtcg caggcgtagg cctcacgctt
    25261 ctcggcgctc gactgggagt ttctgatcac gcccacggtg aggccgagga agcggtagat
    25321 cggcgccatc cagtccgagt cgcgctgcgc cagatactcg ttgaccgtga ccacgtgcac
    25381 gccgttgccc gcgagcgcat tgagataggc ggggagcgtc gcgaccaggg tcttgccctc
    25441 gccggtgcgc atttccgcga tcttgccctc gtgcagcacc atgccgccga tcagctgcac
    25501 gtcgaaatgg cgcatgccga gcgtgcggcg tgccacctcg cgcacgaccg cgaaagcctc
    25561 cacgatcagg tcatcgaggc tcgcgccgtc cttgagccgc gcgcggaact ccgcggtctt
    25621 gccgcgcagg gcctcgtcat cgagcgcctg cagggctggt tcgagggcgg ttatcgcgcg
    25681 ggcggttcgg gcgtagttct tgacgaggcg gtcgtttcgg ctgccaaaga cgcgcgtaag
    25741 cagtgcgagc acggactatc cttgaaaaaa taaaccagat cagtggctta catagcccct
    25801 ggtccgggaa agggcggata ttgtacggat agaccgcgcg cagcggaaat gattcggaaa
    25861 cgccccctga ggggagcagc gggtcagcgc cggatgaagg tcagcgggtc gacctggcgg
    25921 ccctggcgca gtacctcgaa gtgcacgtgc ggaccggtcg agcggccggt ggagccgacc
    25981 tgcgccagca cgtcgccgcg ttgcacggtc tgcccgacag ccaccacgtt gcggcggttg
    26041 tgcgcgtaac gggtgacata gccgttgccg tggttgacct cgaccagctc gccgtaacct
    26101 gcgcgctcgc ccgaccaggt gacgatcccc gcggccacgg ccacgacgtc cgcgccggcc
    26161 tgggccgcga aatcgatgcc cttgtggaac gccccaaggc cagtgaacgg gtcctggcgc
    26221 gagccgaaat aagaggagat gaacccctgg cgcaccgggc ggccctcggg gtggatctgc
    26281 tcgcgcagct ggcgcgcgat cagcacgttc tcgagcgccg cgagctgcgc gtcgcgcagg
    26341 ttgatgcggg cctcgaggtc gttcagcatg ctcgtgatgt cgggaatctg ggcggcgacg
    26401 cccccggccc cgtcctcggg accgccggcg ggcggcgcgg cgtcgaagtt gaattcccgc
    26461 tcgtcgatat tcgccatctg ggcgaggtgc ttgccgaggg catcgaggcg gatcatctgg
    26521 ccattgacct ggcccatgcg catcgagagc gcatcgatcc gctcctgcag cgcgttcttc
    26581 atgttggcga tttccgcctg ctgctgcttg atctgcagcg cccagtgctg ggaatcaccg
    26641 cgcgcgaaca ccttgccgga acgctggccg agttcgaggc cgagcacgaa ggcgatgccg
    26701 aggaccccca ctgccagcgc acccaccgct cccagcgtga ccgggtgggc aaggttcaga
    26761 tggcgggcgc gcccttcgcg ccgggagaag aaaatcagat tcatccgtta ttctcgcacc
    26821 aatggcgggt gaaatatgcc gaatgagcgt aatcgacaca agaagactgg cgcacagact
    26881 ggaaaatcag ggtctgcccg atatggtaaa ccgcgcccgc tgaccgacct gctggcccgc
    26941 ccctcgtcac ccctcaccgc cctgcagcgg cgcgcgcaag ccgctggaga ctggctcagg
    27001 ttcgtgcagg cggccgtggc cacggatctt gccccccatg tcacctccgc ggtcgagcgc
    27061 ggtcatattc tgacgattta tgtcgcctcc gccgcctggg cggcgcggct gcagtttgcc
    27121 gccgacgacc tgctcgcgca ggcccgcgag tacgaccccg acctcggggc cgtgcgggtc
    27181 aaggtccagc ccccggcgcg ggccacgccc gcgcgctagg ccgcgctgct cggcagcgcc
    27241 tcggtaccca cgtagttgat cggcgcgtcc ttcgcggatt cgaacaccac ttcttcccag
    27301 gcctcgctgt cggcatgcac cgcgcgcaac agccggttgt tgaggccgtg gcccgacttg
    27361 tacccgctga attcgccgat caggctgtga ccgagcaggt agaggtcgcc gatcgcgtcc
    27421 aggatcttgt gcttcacgaa ctcgtcctcg tacctgaggc cgtcctcgtt catgatgccc
    27481 tggtcgtcga gcacgatcgc gttgtcgaga ttgccgccga gtgccaggtt gtgcgagcgc
    27541 atcgcctcga ggtcgcgcat gaaaccgaag gtgcgtgccc ggctcacttc cttgaggaag
    27601 gaagtggtcg agaaatccat cgaggcgctc tgcgtgcgcc gcttgaagat cgggtggtcg
    27661 aactcgatcc ggaaattcac cttgaagccg tcgaacgggt ggaactcggc ccacttgtcg
    27721 ccgtcctcaa cgcgcttgtg cttgaggatg cgcacgaagc gcttcggcgc cgcctgctcc
    27781 tcgatgcccg ccgactgcag caggaacacg aaggggcctg cgctgccgtc catgatcggc
    27841 acttcgggcg cgttcacttc gacgatcgcg ttgtcgatgc cgagacccgc gaaggccgac
    27901 atcaggtgct cgatcgtgga aacacgcgcg ttgcccttga cgagcaccgt gccgagcgtg
    27961 gtttcgccga cgttttcggc gcaggccggg atatcgaccg gcgggtcgag atcgacgcgc
    28021 cggaacacaa tgccatggtc gggcggcgca ggacgcaccg tcatcagcac cttggtgccg
    28081 gtgtgcaggc cgacgccgga ggcgcggatg gcattcttca gagtacgttg accgaccatt
    28141 ttggccttag cggtagcgcc cgatccccat caggaaaccg gctggagcga ggcgccggca
    28201 agggcgcacc ggccccggca ggaacggggc gctaccgtag cacaacgtcg cctgcgcgtc
    28261 agtcggcctg gcgccgcagg aaggccggga tgtcgagcac atcctcatcc gcgatgtcgg
    28321 cccgcaaccc gtcgcccacc gcacgctggc gctgcacggt cggacggtcg agcgcggcgt
    28381 aatccgagct gctgagcggt gaacggcgca ccacgcgcat cggcggctcg gcacgcgcgg
    28441 gttcgcgccc ggcgtcgcgc ggacgtgccg tcgacacggg gatacgcccg aggccggttg
    28501 ccaccacggt cacacgcagc tggctggcca gatccggatc gatcacggtg ccgacgacga
    28561 cggtcgcgtc ctcggaggcg tactgcttga cgatggtgcc cacttcctgg aactcgccga
    28621 tcgaaaggtc catgcctgcg gtcacgttca cgagcacgcc atgcgcgccc tgcaggttga
    28681 tgtcttcgag cagcggcgag gacaccgcgg cctcggcggc ctcgcgcgcg cggccctcgc
    28741 cgctcgccga gcccgagccc atcatcgcca tgccggtttc cgacatcacg gtgcgcacgt
    28801 cggcgaagtc gacgttgatc aggcctgggc gcgtgatcag ctcggcgatg ccctggacgg
    28861 cgccctgcag cacctcgttc gccgacttga acgcatcgag cagcgtgacc tgcggtccaa
    28921 gcacggtcag cagcttctga ttcgggatgg tgatcagcga atccacatac ttgccgagct
    28981 cggccatgcc gtgctccgcg accagcatgc gcttgccgcc ctccatctcg aacggcttgg
    29041 tgaccacggc caccgtcagg atgcccatct ccttcgccac ctgcgcgaca accggcgcgg
    29101 cgcccgtgcc ggtgccgtcg cccatgcccg cggtgatgaa gagcatgtcg cagccctcga
    29161 tg
//
LOCUS       DAOQVY010000017        28493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_1947_length_28493_cov_5.913742, whole genome shotgun sequence.
ACCESSION   DAOQVY010000017 DAOQVY010000000
VERSION     DAOQVY010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 28493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28493
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1947_length_28493_cov_5.913742"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PKN91_02980"
     CDS             complement(<1..679)
                     /locus_tag="PKN91_02980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS27066.1"
                     /translation="MHVLYVSDVYFPRVNGVSTSIHTFRTDLATLGVTTTLVAPAYPA
                     GKPATDDAHILRVRSGAVPRDPEDRRMHRGALRATLASPALRDVELVHIQTPFVAHYA
                     GVHAARRLGVPVIATYHTFFEEYLHHYLPVLPRAWGRAVARTFTRSQCAAVDALVAPS
                     EPMREALLGYGVATPIHVLPTGLPADRMREGNGARFRERHGIQAGRPVLLFVGRVAHE
                     KNIEFLVR"
     gene            complement(694..1218)
                     /locus_tag="PKN91_02985"
     CDS             complement(694..1218)
                     /locus_tag="PKN91_02985"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNS27067.1"
                     /translation="MQGLIARFDVAEYRLCRRLNRGADLRPLRRLFVGASRLGDGMAW
                     YALLLALPWMYGLSALRPALAMAVSALAGLLLYKWLKRTFVRERPFIRHRGISLATAP
                     LDRYSFPSGHTLHAVSFTWQAVAFAPELALALVPLAALIAASRVVLGLHYPTDVLVGA
                     ALGAGFGALGAAIA"
     gene            1325..3406
                     /locus_tag="PKN91_02990"
     CDS             1325..3406
                     /locus_tag="PKN91_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27068.1"
                     /translation="MPRIPDRLDLCCLVAALGLLSPGAARAIDTLSVHVGEAIWGDAR
                     ATGIDARLTIGTATQSAAVVRIAKVRPGADQPELRRVTLSCARLTISDPRYACANARV
                     DVQQTPIGPLSLSVSGDFNVGTSTGSARAVIPRLAGGRIEMQGRYAPAGWTAEGRAMG
                     VLVADLRTLIAPLFALPDDWTIDGGATAEFTARGRGGPQTIHGTLALADIDLSNPEST
                     IIAEGAAMTLTAALTARPQGYDLQATLAAGAGQALAGPILLDFGQNPLDVQARGTLAG
                     TTLELTQLEVGAPRLLAMHGSARVALGDAPMLEAGRLVIDALEFPAAYTSLMQITLAT
                     TDFGDLITSGRASGTVDLARNAVERIGIRVEDVSMYNDRAFFMNHVTGEVRWAAAKNA
                     DVLPSWLAWTEGGAYGLSGGASRVQFVTRATNFMLIEPWHMPIFDGAVAIRTLAINDL
                     GGDDMSIRFDAEIEPISMQPLCKAFGWPEFSGSISGSVPGLELRGDELRVAGDIQARV
                     FDGSFVASNLRLVDPLGKWPRFFADLRGRSLDLEQVTRTFAVGSITGRIDVDVLGLEL
                     FAWSPVAFDARIETTKGDRSRHRISARAVSQLSNVGGGGGGVTQALQSGLLQFFDEYS
                     YDRLGLRCRLENDICLMSGVEATDHGYYIVKGSGIPRIDIIGNQGRVNWPQLFSQIAS
                     GIRNSEGVVVQ"
     gene            3480..4088
                     /locus_tag="PKN91_02995"
     CDS             3480..4088
                     /locus_tag="PKN91_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HNS27069.1"
                     /translation="MQKVMLPFKLALACTLFAACVTINVYFPAAAAEKAADQIIDQVT
                     GTATTTGKAEPQMQRAEPSLLRSAAAAVLDTLVPAAHAQEAPNLDIASPEIRAITASM
                     SQRFSQLEKHFASGAVGLTADGLVEVRDQGAIALPDRAAVKRLVAEDNTDRNALYAAI
                     AKANNHPEWEPDIRKTFARRWVERGAKPGWYYQDASGAWKQK"
     gene            4149..5978
                     /locus_tag="PKN91_03000"
     CDS             4149..5978
                     /locus_tag="PKN91_03000"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNS27070.1"
                     /translation="MALDAHAIAVLALTVFAFALFARDDIAIETSSLLIVTLLAAGFI
                     LFPYQGVDGPLEPTDFFLGFGNEALVAICGLVMASQGLVRTGALAPMGRVVTRLWAAS
                     PLVALGAMLVMTAAISAFTNNTPQVALMIPILTSVALHSGTSPSSTLMPMTFASQIGG
                     MGTPIGTSLNLLVIGTAATLGVPRFHMFDFLEPAAIAGGVGILYLWLIAPRLLPKGVA
                     VADTSPRIFAAQMRIRAGGPADGARLADVLRKTGGAMRVKSITRGAGLSIMPLPQTVL
                     AADDQLHVRDTPEHLMEYASVIGGTLYSGDVAVDAEHPLRADEQQTAELVVTPGSMLV
                     GQSLDGIDFDKRYQLVALARHRPGDVEAMSAMGGEPIDLHAEQLGVGDVLLVQGPAEQ
                     IAAVKRSGELLVLDATTDVPETRKAPLALAIMGMIVLAATLRIAPVSIAALVGVLLMV
                     FTGCLKWRDATRSLDSSMIFLTVAAIALSLALVKTGAAAFLASVLVSAMSSLSPPVIV
                     SLTMLFMAVLANFVSNSAAAVIGTPIAIEGARQLGLAPEPFVLAVLFGVNMAYATPLA
                     DNCNLLVFSAGNYRFKDFVRAGVPLMIIMWLTLSWLLPRYFPF"
     gene            complement(5994..6785)
                     /locus_tag="PKN91_03005"
     CDS             complement(5994..6785)
                     /locus_tag="PKN91_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004753277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS27071.1"
                     /translation="MTTGSPALGHVWWVGFKTILLRETHRILRIWGQTIVPSAVTSTL
                     YFVIFGALIGKRIGPMGGVNYMQFIAPGLIMMGVITNSYANVVSSFFGAKFSKHVEEM
                     LVAPLPAWVIVTGYVGGGVIRGLLVGAAVTIISLLFAEIVIHHLLIIIAAVVLTAIVF
                     SLGGFLNAMYAKNFEQVNWIPSFVLTPLTYFGGVFFSISLLPVWAQKLSYTNPILYMV
                     GAFRYGFLGTSDVDIVLAFALMIAFAAVMFAIAVVLMNRGTGMRE"
     gene            complement(6782..7705)
                     /locus_tag="PKN91_03010"
     CDS             complement(6782..7705)
                     /locus_tag="PKN91_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS27072.1"
                     /translation="MYALSARGLVKTYDNGVQALKGVDLQVERGDFFALLGPNGAGKT
                     TLIGIATSLVNKSGGSLEVFGHDIDRELDAAKACIGVVPQEVNFNMWETPLAIVVNQA
                     GFYGIDRKLARQRAEYYLGKLSLWDRRNTTSRALSGGMKRRLMIARALMHEPRLLILD
                     EPTAGVDIEIRRSMWDFLREINAQGTTIILTTHYLEEAETLCRNIAIINGGQIVERDR
                     MASLLRRLKSEVFVLSLRDAITAAPPLDGYIVTRIDDHTIEVEVSDEQNLNEIFARLT
                     AQGVIVQSMRNKVNRLEEIFMRLVDGRRAPR"
     gene            complement(7754..8356)
                     /locus_tag="PKN91_03015"
     CDS             complement(7754..8356)
                     /locus_tag="PKN91_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27073.1"
                     /translation="MDTGPSATALAPLAEINELALELMTRRAAGVPAGLHAVLRDSVE
                     LWRALTPEQGRRIAGCPFLLVEIDVAALSAGGGVRDSGEGEHWFEEQSLPRLAYLTLT
                     YAWHIARSRPLAARIVLGLHEADSAWLAGLTLRELGACIEIAPALLRPRWLAHPEIWR
                     HLLSAAASAAPAEFELARLRGMQLLAAPWWPADPRRPRLR"
     gene            8469..9107
                     /locus_tag="PKN91_03020"
     CDS             8469..9107
                     /locus_tag="PKN91_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FlhC family transcriptional regulator"
                     /protein_id="HNS27074.1"
                     /translation="MRISDDRYSRDRLRFDLALRLIGHEARTHTIRAWTGLTEDRVRK
                     LYRTYIAEHPGQKVARRRGKSPQRCSYFLRTPRVQQEAAVLAGLYCMVGIIAQGARLD
                     PRMLSVAQGELLCVAYETYLALVKSPQISFEHAAFLAIALARGDELALRHCGSCGALA
                     LADRYTLRATVCAVCAESAAPLSALRIDGRRAPPAAARGTPAPARLGPVDTG"
     gene            9149..10075
                     /gene="nudC"
                     /locus_tag="PKN91_03025"
     CDS             9149..10075
                     /gene="nudC"
                     /locus_tag="PKN91_03025"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HNS27075.1"
                     /translation="MQQQRKHFNHFAGPYLDRNAWEREDAGWLATARRDSSTRYLIAR
                     GTAHLVVHEPAPHIVFLGGDHPRIALAQERQTVLLGWFEGARCVLVEDDADGDIAPPG
                     ARFEELRPLSATLPEREAALIAYARALSIWRARHRYCGACGAPTRPLRAGHVLGCSNP
                     DCGQQLFPRIDPAVIVLVTDGDRALLGRQASWPKNRYSTIAGFVEPGESIEDAVAREV
                     LEETGIAVGEVTYHSSQPWPFPSSLMLGFHARAEGGEIVCRDGELEDAQWFSRAEIAA
                     GHPLLSPRTSISWRLIESWFDAGWHTPLARIG"
     gene            10075..10650
                     /locus_tag="PKN91_03030"
     CDS             10075..10650
                     /locus_tag="PKN91_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNS27076.1"
                     /translation="MEISTASALLSVALGIGLAAAVGLRVFLPLLITAVAGATGHVDL
                     APSFEWLATTPALVMLGVATVLELFAYYIPGVDNLLDVIATPTAVVAGAVVAAAVMAD
                     LPPLVKWTTAIIAGGGVAGLTQGVTALVRAKSTATTGGLGNSAVATGELGGSIVLTVL
                     ALAAPVIAVALVVLVVWLAVRLVRRLLRPVR"
     gene            10661..11464
                     /locus_tag="PKN91_03035"
     CDS             10661..11464
                     /locus_tag="PKN91_03035"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HNS27077.1"
                     /translation="MCLLVLAWRAHPRYRLVVAANRDEYHGRPAAAMGPWNDLPILAG
                     RDLEAGGTWLGVDRARRFGVVTNFREMVRRPAGAPSRGGLVPAWLKDTRGVADHAASL
                     RATAADYAGFNLLFADRDSLYYLSNRLESFSRPLASGVHGLANHYLDTPWPKLLRVRH
                     GLERWLGERNADPRSLFALLADRAPASDGDLPDTGLSPEWERALSAPFVVHGEYGTRC
                     STVLALGHDDSLQIEEQSWDATGRLTDVASYRLGPEDWPAGNPPAPAQL"
     gene            11499..13256
                     /locus_tag="PKN91_03040"
     CDS             11499..13256
                     /locus_tag="PKN91_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HNS27078.1"
                     /translation="MPGPQRDTRSATARCLLALCALVATSAHADIDIVIEGVEGPVRR
                     NVLAFLSLERYKDRDRLREDTIERLHERAEREVRAALRPFGYYEPTVRSSVEARPNGQ
                     DWRIEIHIEPGPPVILETVEVRVTGARANDPVFTNLLAGMPLRAGAQLHHSDYEQVKS
                     DLQRTAAAYGYLDARLLRHELLVDPATRTASAHIEFETGERYRFGPTEIAQDVIRPEL
                     MRRFLRYEEGQPFDAGELLRTQFALDDSAYFASVEVLPQERDPATFTVPIRIAATPNR
                     QSRWTFGIGYGTDTEARGTIGWDNRIVNALGHRFRAEVKLAEISQRVSGRYVWPIGDP
                     ALEKLEVEGTWEHQTLADLDTYNTEARAGITRVPHRWQRVYYTRLVRAITEDATSRVE
                     SLLLIPGFSVASVPKGYLGEPLFGRQLYAELRGSHTGLGADTDYLQLLAASEHVFSIA
                     PAWHLLLRAEIGTTWADDFDGVPGSERFFAGGDRSVRGFGFNDLSPVDENGEKTGGKH
                     MVAGTVEVIRDLPRNLGLAVFVDAGNAFNAVSEGLEYSAGIGLRFRLPIATVGFDIAQ
                     ALSVSGATPRLHVNFSPKL"
     gene            13256..16981
                     /locus_tag="PKN91_03045"
     CDS             13256..16981
                     /locus_tag="PKN91_03045"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNS27079.1"
                     /translation="MARPVSILLVLAIALLCALPFALIYFIAFTPQGLAMIVARMPER
                     VGPVSLDIGTVSGTIAGGFELSRFELDHERVHLVAEDIQARLAVAPLLWQKIEVPEVH
                     TRNLLIEVKTRPQRPRKGTPRFLPRLLRIETDDARAALLTLVSTKGVRTEFADIRASG
                     VVRHRSIRVFGAAARFQGMDLTANGTVRAASPMRIDASARVEWFAPKSTRWIAHVAGD
                     GDLDSLPFTGSIEAPFRAAVSGAAETLTRAWKIVGSAQISQLDIADFGGGGALGEIDG
                     RVKVGITADGIAADGVMHPAGLEAGDFDVQFNGRYAARTLQVRHAQFRHRGSGALATT
                     AGEIRIDEAGPSLDLAGEWREFRWPLAAAEPAVHSAQGSYQLAGAMPYAITASGAVVA
                     ADLEPMQVVLEGRLAGDRLDADVLRVEGYEGRADLRGAVQWQPSEAWQVAGRIAGVNT
                     GRVNGRLPGRVSLDIDARGEGFSPEGSLDLRFARLGGKVRGGSASGAGRIIRRGAMLG
                     FHDFDLRLGAARAQLEGDISPDAYSLRFDFDADDLALLDAGATGRLAARGTVGGSRAA
                     PAIDATLRGLAIDYAGVQVDGIEGRVRFDGRAGQALDIDLQARGITRGARKIERFTVT
                     AKGTTEQHEVAMTTNAAPVAIGARGPGSYRAGTWQLQFRELDLENGDEAELHLSAPSL
                     LTVSRDSQRLETTCLRGTVAELCATGNHAPQEWAVTLSAKRLPISTFTAGLTPGVTYE
                     GSVDIEAAASARDNEPWRGSLRADLLDVQTRRRLANNRVDVTVIGGGVITGSATAESI
                     VVDARLDAGASGDLTGNAQIARSAEDWRDWPLQGRLDARTDSLGFIAVLVGTIDRSSG
                     SLSTNLRLSGTLGAPRFEGLLSIRDGQFDIYQVNLPLRAVTLDAHFLKDTLTLESTAT
                     AGDGSLAAGGALTWRKGLPYGKLTMRGENLRIANVPEARIFASPDLVFDVDGRHIDVT
                     GAVLVPNARLDPADITGAVFSSADERIVGEPVVDPSKRFTVTTRVNIALGDRVTIDTS
                     GLSGRLTGALEARTDVDDVARGTGELAVAEGRYLAFGRRLDIEHGRLIFNNGPLADPG
                     IDIRASREFPDIRAGANIRGTLRAPRLTLFSEPQIPQSQILSLLIAGGSLESAQAAEG
                     GRTSNPFAVQGGAILAQQLGQIVGLEDVSLESSLNNETSLVLGRYLTPRLFVSYGISL
                     TEAINTIKMRYTLGDDWTVKLESGAVQSADLQYTIER"
     gene            17052..17504
                     /locus_tag="PKN91_03050"
     CDS             17052..17504
                     /locus_tag="PKN91_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27080.1"
                     /translation="MHTSPEQAAHRRDTITRLIRSGTVRKQADLVRLLKAEGHAVTQS
                     SISRDLREIGVLKAADGYVLPAGDARPAGDFTALAQFVRGLRFAGPALTVLRTSVGAA
                     QSVAVAIDQANWPEVAGTISGDDTIFIATAGARDQAALARRLRSFFPI"
     gene            17515..18729
                     /gene="argG"
                     /locus_tag="PKN91_03055"
     CDS             17515..18729
                     /gene="argG"
                     /locus_tag="PKN91_03055"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNS27081.1"
                     /translation="MTQPIVLAYSGGLDTSFLVPWLAEQYGRPVITVTVDTGGIDAAA
                     ARTLEERALALGAVAHHQVDARADYFEQVLRFLVMGNVRRGQLYPLCVGAERVMQAQT
                     IARMAEKLGTRSIAHGCTAAGNDQVRFEVALRTLAPELEIIAPVRDRAFQRQEELDYL
                     RARGLPVPPFGAAYSINRGLWGVTIGGRETLTSEGSIPEEQWVLSKDAFTNPRAPQRH
                     TIGFAAGRPISVDGHELSPVAAIETLEALAAPFGIGRGIHLGDTIIGTKGRVAFEAPA
                     AEVLLVAHRELEKLVLTARQQRIKESVAQPYGDLVHEGQLLDPVCRDIEALFLSSQER
                     VTGTVSVLLRPGSVFVEGVSSPWSLMAASKGVYGESVGEWSAADALGFSKLVALPGVF
                     HTRAGKLAGSAR"
     gene            18729..19808
                     /locus_tag="PKN91_03060"
     CDS             18729..19808
                     /locus_tag="PKN91_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27082.1"
                     /translation="MMKSVVVDKIASITQAAGLSRELRISTDIPSEEGVIVVVEILTN
                     KSTYNTLELTSGRMAKLGKGDIVVGALGHRKALFGYSGHVPESLKAGDVIQMLNIGGV
                     LGVCDSVNPDKGTPFDCRVLGVVLQFPYLGERIGVPARAGYRNLDYATALDTRGVPVV
                     ALAGTCMEAGKTAAACSIVSRMRHRGLVVDAFKATGVSLRRDILAMADAGARNTMIFT
                     DLGVITTTPKSGPALTRTMLTELAATRPDVIVFELGDGLMGTYGVDAILSCDDIRTAL
                     TGVVLSANDPVAAWGGVKMLRERFGIEPCVVTGPATDNAVGVDIIRQQMNVAAFNALS
                     SGAALGDHIIEAIGLHAKPVEALAG"
     gene            19805..20884
                     /gene="argC"
                     /locus_tag="PKN91_03065"
     CDS             19805..20884
                     /gene="argC"
                     /locus_tag="PKN91_03065"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNS27083.1"
                     /translation="MSGGRIPAIVLGGTGYVSGELLRLLAAHPHFALAGVLSDSQPGE
                     AVAKAFRHLAPALGDLSFGTQAEIESLIGTLPRLALFGAAPHGVSAALIDALLTRAEA
                     AGSDVHVVDISADFRYRDAAAYEAVYKHPHGAPGRLAQFACAVPEHLARAPTRHVAHP
                     GCFSTATLLASVPLLALDLVTPTLFVAGVTGSTGSGRKPSEGTHHPQRHGDFYTYNAL
                     AHRHTPEVAACAQAASGVAADFAFVPHSGPFARGIHVTVQASLKARPGAAAPDTAAVL
                     AALRDFYRGAPFVQVAAEAPHVKDVAASNYARLSAAANGRTVAVTCVVDNLNKGAAGG
                     AVQWMNRLFALPEDAGLTAPAAGWT"
     gene            20881..22008
                     /locus_tag="PKN91_03070"
     CDS             20881..22008
                     /locus_tag="PKN91_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNS27084.1"
                     /translation="MSALAQVFAQYPLEVEHAEGVWLYSRDGRRVLDLYGGHAVAALG
                     YGHAGWTRALQAQAASCNFQSNAVPMAVRERAAVRLLRFAGGHFDSVFFVNSGAEANE
                     NALKMAFRMTGRPQVAAVEGSFHGRTAAAGAITWGAMGKWHAFPRAPFDVSWIPRRES
                     GAVAQHVTEDTAAVIVEPVQGVGGAFDMGSDFLGALRRRCDEVGALLIFDEVQCGVGR
                     TGYAFAADLYGVRPDMITTAKALGNGFPCAALLMTPRVSQAVTLDALGTTFGGGPMAG
                     AVIEAVIEAIESEELLANVRRVSAYIRTRCAVGPVTGFQGEGFLLGLRMNRPARDIQA
                     ALLARDILTGTSGDPQVLRLLPPYVLEEQHVDLLAEALASL"
     gene            22005..23039
                     /locus_tag="PKN91_03075"
     CDS             22005..23039
                     /locus_tag="PKN91_03075"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HNS27085.1"
                     /translation="MTRNPDMQRFLDLADFSRDEIVSLLALARRLEAHPEPQALAGRI
                     LGLVFFNPSLRTLASFQAGMARLGGSSFVITPGQGTWQLETRLGAVMNGAAAEHVREG
                     LPVLASYCDALGIRAFAEGKDLADDIAESRFGAMASVIDKPLINLESAMNHPCQALAD
                     WKTMDDLAIPRQAKFVLSWVQHPRALPLAVPAATVHMAAMRGMEVVVLRPEGYALPPQ
                     VMAKARAAAAASGGSVRETSERGEALAGAQVLYAKEWGSTAHYGDAEGDALLRAQLTD
                     WCVTPEWFEPADPQCRLMHCLPVRRNVAIADTVLDGPRSIVRHEAYNRMTAQMAVLHR
                     MLSQGVRRPT"
     gene            23036..23971
                     /gene="argB"
                     /locus_tag="PKN91_03080"
     CDS             23036..23971
                     /gene="argB"
                     /locus_tag="PKN91_03080"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNS27086.1"
                     /translation="MMKHRADQALAVRSLRSAAPYIRMYKGKTFVIKAGGAVFGDAQA
                     TRALIEQIAILHYFGVRVVFVHGGGPQLSELTEALGVPTRMVQGRRITDQKSIDATSM
                     VLNGLINTRILAICRELDISAVGVSGVDASLVTAHRRPVVRMPDGENVDFGFVGDIDA
                     VDTTVLRKLLDNGLMPVVSPLSADAGGVLLNINADTVAAAIGAALGAEKLILCTGAPG
                     ILERVEDPTSVVSYTDLNGLKRMREDGSIKDGMLPKAKAIEDAIRGGVRRVHVIAYTS
                     PESILAEVFTNEGTGTLIVADINALSVAEQQSGAA"
     gene            23968..25155
                     /gene="argH"
                     /locus_tag="PKN91_03085"
     CDS             23968..25155
                     /gene="argH"
                     /locus_tag="PKN91_03085"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNS27087.1"
                     /translation="MTRLWDKGAPLDERVLAYTAGEDYALDERLVRYDVAASIAHAAM
                     LHAQKLLTDADFVAIREGLTAIGEAHERGEWRIELADEDGQSALERRLTARIGAAGGR
                     VHLGRSRNDQVLTALRLYLRDAIEQLRTGASQVADALDALAARGGEIALPGYTHMQQA
                     MPSTVALWAGGFAAELRDDAEGLRLAHRRTAKCPLGSAAGYGTPGLPLDRDATRAALG
                     FAVTHEPVTAVQLSRGKAETQLLFEVALTMQDLGRLAADLLLFYTQEFAFVTLPDEFT
                     TGSSIMPQKRNPDVFELVRGRTATAHANLVEALGIFAKLPSGYQRDLQLLKLPLFRGI
                     DIALQTLDIMAAAIGAVHFRRENIALDPAIHAAEEANALVVKEGIPFREAYRRVSARL
                     RKV"
     gene            25174..25605
                     /locus_tag="PKN91_03090"
     CDS             25174..25605
                     /locus_tag="PKN91_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HNS27088.1"
                     /translation="MSIWFRQPTREQLNALSANTLVAHLSIEFTEIGDDFMRATMPVD
                     ARTHQPFGVLHGGASVALAETLASTAASCCIDTARFMCLGQEINANHLRPVRSGLVTG
                     TTRPYHIGARSQVWGIELRDAAGHLACVSRITMATVERRQV"
     gene            25633..26049
                     /locus_tag="PKN91_03095"
     CDS             25633..26049
                     /locus_tag="PKN91_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27089.1"
                     /translation="MNEANQPTAGLDPGLVTYTNIIYGLHALSVLIGVTGPATVIGSF
                     VFGLPSIIAVIMNYVRQSEVRGTWLESHFRWQIRTFWFAFLWIVVAAIVSAPLVLLFG
                     LGIVTFYLAAVIIAIWIVYRVARGWIALRDKRTMFL"
     gene            26049..26252
                     /locus_tag="PKN91_03100"
     CDS             26049..26252
                     /locus_tag="PKN91_03100"
                     /inference="COORDINATES: protein motif:HMM:NF025012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HNS27090.1"
                     /translation="MCRALAAATLALALLAACGQKGPLYLPDQGGNVVVRPAAEPTPA
                     PAPVDDDADPKSGRKPPEAAPAP"
     gene            complement(26263..26460)
                     /locus_tag="PKN91_03105"
     CDS             complement(26263..26460)
                     /locus_tag="PKN91_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27091.1"
                     /translation="MLAFLFWLLLLVICWPIALIGLLLYPLVWLVLLPFRLLGIAVNG
                     VFELLKSIILLPARVLRGKPV"
     gene            26534..26686
                     /locus_tag="PKN91_03110"
     CDS             26534..26686
                     /locus_tag="PKN91_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNS27092.1"
                     /translation="MQIGELAKSAGVPIDTVRYYERQGILPAPRHGGVKKAATAKLAA
                     LSGEES"
     gene            26683..>28493
                     /locus_tag="PKN91_03115"
     CDS             26683..>28493
                     /locus_tag="PKN91_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNS27093.1"
                     /translation="MTHEHHEHAHHSHEGRVTDPVCGMKVDPQKTPHHASHGGAAYYF
                     CGARCRERFIADPGKYLAAQATGMPPESPAAPAGTIYTCPMHPEVRQEGPGICPKCGM
                     ALEPEMPSLDDEDNPELRDFTQRFWWSLPLSAVVFVLAMFGHHVPGLSIGARTWIEFA
                     CATPVVLWAGRPFLERWWQSLRNRSPNMWTLIGSGVIAAYGYSVVATLAPGIFPDSFR
                     EHGRVGVYFEAAAVIISLTLLGQLLELRARSKTSAAVKALLGLAPKTARRLRDDGSEE
                     DIELAHVHVGDRLRVRPGEKVPVDGVVLEGRSHIDESMLTGEPIPVEKAAGAAVIGAT
                     LNGSGSLVIRAEKVGSATVLANIVQLVAQAQRSRAPMQRLADRVAYWFVLAVFAVAIA
                     TFFIWGLAGPDPSWTYAVLNAVSVLIIACPCALGLATPMSIMVATGRGAHSGVLFRDA
                     EAIENLRAIDTLIVDKTGTLTEGRPAFRAAVSAPGWTEDELLRLAASLDQASEHPLAE
                     AIVAEARRRGLTLEPPEGFESSTGVGVHGRVGARRLVIGNTALMQQSDIDTASLANDA
                     EALRQDGASVMWIGVDGALAGLIAVADPVKASTPAAI"
BASE COUNT         4387 a        10047 c         9642 g         4417 t
ORIGIN      
        1 tcctgaccag gaactcgatg ttcttctcgt gcgcgacccg gccgacgaac agcagcaccg
       61 gcctgcccgc ctggatgccg tgtcgctccc ggaagcgcgc gccattgccc tcccgcatgc
      121 ggtcggcggg caggcccgtc ggcagcacgt ggatcggcgt agccactccg tacccgagca
      181 gcgcctcccg cataggttcc gacggcgcga ccagtgcgtc caccgccgcg cattgtgagc
      241 gcgtgaaggt gcgtgccacg gcgcgtcccc atgcgcgcgg caggacgggc aggtagtggt
      301 gcaggtattc ctcgaagaac gtgtggtagg tggcgatcac cggcacgccc aggcgacggg
      361 ccgcgtgtac ccccgcatag tgcgcgacga acggcgtctg gatgtggacc agttcgacgt
      421 cgcgcagagc cggcgatgcg agagtcgcgc gcagcgcgcc gcgatgcatg cgccggtcct
      481 ccgggtcgcg gggcaccgcg cccgaacgca cgcgcaggat gtgcgcatcg tcggtggccg
      541 gcttgccggc cgggtaggcc ggcgcgacga gcgtcgtcgt gaccccgagc gtcgcgagat
      601 cggtccggaa cgtgtggatc gatgtcgaca cgccattgac gcgcggaaaa tatacgtcgg
      661 agacgtacag gacgtgcatg ggggcgggcg cggtcaggcg atcgcggcgc cgagcgcgcc
      721 gaatcccgcg ccgagcgcgg caccgaccag tacatcggtc gggtagtgca gcccgaggac
      781 cacgcgcgat gcggcgatca gcgccgcgag cggcaccagc gcgagggcga gctccggcgc
      841 gaaggccacc gcctgccagg tgaacgaaac ggcatgcagg gtgtggcccg aggggaagct
      901 gtagcggtcg agcggcgcgg tcgcgagcga aatgccgcga tggcggatga agggacgctc
      961 gcgcacgaag gtgcgtttta gccacttgta gagcaacagg cccgccagcg cactcacggc
     1021 catcgccaaa gcggggcgca gggccgacag cccgtacatc catggcagcg ccagcagcag
     1081 ggcgtaccac gccatgccgt caccgaggcg gctcgcgccg acgaacagcc tgcgcagcgg
     1141 ccgcaggtcg gcaccgcggt tcagtcgccg gcacagccgg tactcggcga catcgaagcg
     1201 cgctatcagt ccctgcatgt gtctcgttcc ctgactcatg cggcgcagga tgccagcccg
     1261 tcaagtcaac cttgttacgg cggtattacc gctgcatgac aggggcgcgg ttacactggc
     1321 ggacatgccg cggatccccg atcgcctcga cctctgctgt ctcgtggcgg cgctcggcct
     1381 cctgtcgccg ggcgcagcgc gcgcgatcga tacgctcagc gtgcatgtcg gggaggctat
     1441 ctggggcgat gcgcgcgcca ccggcatcga cgcgcggctc accatcggga ccgcaacgca
     1501 atcggccgcg gtggtgcgca tcgcgaaggt gaggcctggc gcagaccagc cggaactgcg
     1561 gcgggtcacg ctcagctgcg cgcgcctcac gatcagcgat ccgcgctacg cctgcgccaa
     1621 cgcgcgggtc gacgtgcagc agaccccgat cggccccttg tcgctgtccg tgtcgggtga
     1681 cttcaatgtg ggcacgtcga ccggcagcgc acgcgcagtc atcccgcgcc tcgcgggcgg
     1741 ccgcatcgag atgcaggggc gctacgcgcc tgcgggctgg acagccgagg gtcgtgccat
     1801 gggtgtgctc gtcgccgacc tgcgcacgct gatcgccccg ctgttcgccc tgccggacga
     1861 ctggacgatc gacggcggcg ccacggccga attcaccgcg cgcggccgcg gcgggccgca
     1921 gacgatccac ggcaccctgg cactcgccga catcgatctg tcgaacccgg agagcacgat
     1981 catcgccgaa ggggccgcga tgacgctgac cgccgcgctc actgcgcgcc cgcagggcta
     2041 tgacctgcag gccacgctgg ccgcgggcgc tggccaggcg ctcgccggcc cgatcctgct
     2101 cgatttcggc cagaacccgc tcgacgtgca ggcccgcggc actctcgccg gcaccacgct
     2161 tgaactcacg cagctcgaag tcggcgcgcc gcgactgctc gccatgcatg gttcggcacg
     2221 cgtggcgctc ggagacgccc cgatgctcga ggccggccgc ctcgtgatcg acgcgctgga
     2281 gttcccggcg gcctacacca gcctgatgca gatcacgctc gccaccaccg acttcggcga
     2341 cctcatcacc agtggccgcg cgagcggcac ggtggacctc gcccgcaatg ccgtcgagcg
     2401 catcgggatc agggtcgagg atgtctcgat gtacaacgat cgtgcgttct tcatgaacca
     2461 tgtcaccggc gaagtgcgct gggcggccgc gaagaacgcc gatgtcctgc cgtcatggct
     2521 cgcgtggacc gagggcggcg cctacgggct gtccggcggc gcatcgcgcg tccagttcgt
     2581 cacgcgcgcg acgaatttca tgctgatcga accctggcac atgccgatct tcgacggggc
     2641 cgtcgcgatc cgcacgctgg cgatcaatga cctcggcggc gacgacatga gcatccgctt
     2701 cgatgccgag atcgagccga tcagcatgca gccgctgtgc aaggccttcg gctggccgga
     2761 gttctcgggg agcatttcgg gcagcgtgcc tgggctcgag ttgcggggtg acgagctgcg
     2821 cgtcgccggc gacatccagg cgcgggtgtt cgatggttcg ttcgtcgcga gcaacctgcg
     2881 gctagtcgat ccgctcggca agtggccccg cttcttcgcc gacctgcgcg gccgcagcct
     2941 cgacctcgag caggtcaccc gcacctttgc ggtcggctcg atcaccgggc gtatcgacgt
     3001 cgatgtcctg ggactcgaac tgttcgcgtg gtcgcccgtc gcattcgatg cgcgcatcga
     3061 gacgacgaag ggcgaccgct cgcgccaccg gatcagtgcc cgtgcggtca gccagttgtc
     3121 caacgtcggc ggcggcggcg gcggcgtgac acaggcgttg cagtccggcc tgctgcagtt
     3181 cttcgacgaa tacagttacg atcggctcgg cctgcgctgc aggctcgaga acgacatctg
     3241 cctgatgtcc ggggtcgagg ccacggacca tggctactat atcgtcaagg gcagcggcat
     3301 cccgcggatc gacatcatcg gcaaccaggg ccgcgtcaac tggccgcaac tgttcagcca
     3361 gatcgcctcg ggtatccgca acagcgaggg cgtggtggtg cagtaacatg gcaggtacac
     3421 tggttcaggc tccattcatc cggtggcccc acaatgactc tgtgtttagg gagaggacca
     3481 tgcagaaggt aatgctgccc ttcaaactcg cgctcgcctg cacgctgttc gcggcctgcg
     3541 tgacgatcaa cgtgtacttt ccggccgccg ccgccgagaa ggccgccgac cagatcatcg
     3601 accaggtcac cggcaccgcg accacgaccg gcaaggccga gccgcagatg cagcgcgccg
     3661 aaccgtccct gctgcgcagc gccgccgccg ccgtgctcga cacgctggtg ccggccgccc
     3721 acgcccagga ggcccccaat ctcgacatcg catcgcccga gatccgcgcg atcaccgcgt
     3781 cgatgtcgca gcgcttttcg caactcgaga agcacttcgc ttccggcgcc gtgggcctca
     3841 ccgccgacgg gctcgtcgaa gtgcgcgacc agggcgcgat cgcgctgccc gatcgcgcgg
     3901 ccgtgaagcg cctcgtcgcc gaggacaaca ccgaccgcaa cgcgctctat gccgcgatcg
     3961 ccaaggcgaa caaccacccg gagtgggaac cggacatccg caagactttc gcgcgccgct
     4021 gggtcgagcg cggcgccaag ccgggctggt actaccagga cgcgtccggc gcctggaaac
     4081 agaagtagcc tgcgcagcac ggtagctgga ccggcggtgt gcagccgccg gtccgcgagc
     4141 ttccctccgt ggccctcgat gcccatgcga tcgccgttct tgccctgacg gtgttcgcgt
     4201 tcgcgctgtt cgcgcgcgac gacatcgcga ttgagacttc gagcctgctg atcgtcacgc
     4261 tgctcgcggc gggtttcatt ctctttccct accagggggt ggacggcccg ctcgagccca
     4321 ccgatttctt tctcggtttc ggcaacgagg cgctggtcgc gatctgcggc ctcgtcatgg
     4381 cgagccaggg cctggtgcgg accggggcgc ttgcgccgat gggccgcgtg gtcacgcggc
     4441 tgtgggccgc gagcccgctg gtcgcgctcg gcgccatgct cgtcatgacc gcggcgatca
     4501 gcgccttcac caacaacacg ccgcaggtcg cgctgatgat cccgatcctg acgagcgtgg
     4561 cgctgcattc cgggacctca ccctcctcga ccctgatgcc gatgaccttc gcatcgcaga
     4621 tcggcggcat gggcacgccg atcggcacct cgctgaacct gctcgtgatc ggcaccgccg
     4681 cgaccctcgg cgtgccgcgc ttccacatgt tcgactttct agagcccgca gcgatcgccg
     4741 gtggcgtcgg catcctctat ctgtggctga tcgccccgcg gctgctgccg aaaggcgttg
     4801 cggtggccga cacctcgccg cgcatcttcg ccgcgcagat gcgcatccgg gccggcggtc
     4861 ccgccgacgg tgcgcgcctc gccgacgtgc tgcgcaagac cggcggcgcg atgcgcgtga
     4921 agtcgatcac gcgcggcgcg ggcctgtcga tcatgccgtt gccccagacg gtgctcgccg
     4981 ccgacgatca gctgcacgtg cgcgatacgc ccgaacacct gatggagtac gcaagcgtga
     5041 tcggcggcac cctctactcc ggtgatgtgg ccgtcgatgc cgaacacccg ctgcgcgccg
     5101 acgaacagca gaccgcggag ctggtcgtga ctcccggctc gatgctcgtc ggccaatcgc
     5161 tcgacggcat cgacttcgac aagcgttacc agctcgtcgc actcgcgcgg caccggccgg
     5221 gtgacgtgga agcgatgtcg gcgatgggcg gtgaaccgat cgacctgcac gccgagcagc
     5281 tcggcgtcgg cgacgtcctg ctcgtccagg gacctgccga gcagatcgcc gcagtcaagc
     5341 gcagcggcga actgctggtg ctcgatgcca ccaccgacgt gccggagacg cgcaaagcac
     5401 cgctcgcact cgccatcatg ggcatgatcg tgctcgccgc cacgctgcgc atcgcgccgg
     5461 tgtcgattgc cgccctggtc ggcgttctgc tgatggtgtt caccggctgc ctcaagtggc
     5521 gcgatgcgac ccgctcgctc gattcgtcga tgatcttcct caccgtcgcc gcgatcgcgc
     5581 tgtcgctggc gctggtcaag acgggcgccg ccgcgttcct cgcaagcgtg ctcgtgtcgg
     5641 cgatgtcatc gctgtcgccg ccggtgatcg tcagcctgac gatgctgttc atggcggtgc
     5701 tggcgaattt cgtgtcgaac agcgccgccg cggtcatcgg cacgccgatc gcaatcgagg
     5761 gcgcgcgcca gctcggcctc gcaccggagc ccttcgtgct cgcggtgctg ttcggggtca
     5821 acatggccta cgccacgccg ctcgccgata actgcaacct gctggtgttc agtgccggca
     5881 actaccgctt caaggatttc gtgcgggccg gcgtgccgct gatgatcatc atgtggctca
     5941 cgctcagctg gctgctgccg cgctacttcc cgttctgacg acggtacctc accctactcg
     6001 cgcataccgg tcccgcggtt catcagcacg acggcgatcg cgaacatcac ggcggcgaag
     6061 gcgatcatca gcgcgaacgc cagcacgatg tccacatccg aggtgccgag aaagccgtag
     6121 cggaatgcgc ccaccatgta caggatggga ttggtgtagg agagcttctg cgcccacacc
     6181 ggcagcaggg atatcgagaa gaacacaccg ccgaaatagg tgagcggtgt cagcacgaac
     6241 gacgggatcc agttcacctg ctcgaagttc ttcgcgtaca tcgcgttgag aaagcccccg
     6301 agcgagaaca cgatggcggt aagtacgacc gccgcgatga tgatcaacag atggtggatc
     6361 acgatctcgg cgaacagcag cgagatgatc gtcaccgcgg cgcccaccag caggccgcga
     6421 atcaccccgc caccgacata gccggtgacg atcacccacg cgggcagcgg cgcgaccagc
     6481 atttcctcga cgtgcttgct gaatttcgcg ccgaagaacg acgacacgac gttcgcgtag
     6541 gagttggtga tcacgcccat catgatcagg cccggcgcga tgaactgcat gtagttgacg
     6601 ccgcccatcg ggccgatgcg cttgccgatc agcgcgccga agatcacgaa gtagagcgtc
     6661 gaggtgaccg ccgagggcac gatcgtctga ccccagatgc gcaggatccg gtgggtttcg
     6721 cgcagcagga tggtcttgaa gccgacccac cacacatgcc cgagtgcagg actgcccgtg
     6781 gtcaccgcgg cgccctccgc ccgtcgacga ggcgcatgaa gatctcctcg aggcgattca
     6841 ccttgttgcg catcgactgc acgatgacac cctgcgcggt gaggcgcgcg aagatctcgt
     6901 tcaaattctg ttcgtccgag acctcgacct cgatggtgtg gtcatcgatg cgtgtcacga
     6961 tgtaaccgtc gagcggcggc gctgccgtga tcgcatcacg caggctgagc acgaacactt
     7021 cgctcttgag ccggcgcagc agactcgcca tgcgatcgcg ctcgactatc tgcccgccgt
     7081 tgatgatcgc gatgttgcgg cagagggtct cggcttcctc gaggtagtgc gtggtgagaa
     7141 tgatggtggt gccctgcgca ttgatctcgc gcaggaaatc ccacatcgaa cgacgaatct
     7201 cgatgtcgac gccggcggtg ggctcgtcga ggatcagcag gcgcggctcg tgcatcagcg
     7261 cgcgcgcgat catcaggcgc cgcttcatgc cgccggacag cgcgcgcgac gtcgtattgc
     7321 ggcggtccca cagcgagagc ttgccgaggt agtactcggc acgctgcctg gcgagcttgc
     7381 gatcgatgcc gtagaagccg gcctggttga cgacgatcgc gagcggcgtc tcccacatgt
     7441 tgaaattgac ttcctgcggc accacgccga tgcacgcctt ggcggcatcg agctcgcggt
     7501 cgatgtcgtg gccgaacact tcgaggctgc cgccgctctt gttgacgagc gaggtggcga
     7561 tgccgatcag cgtggtcttg ccggcaccgt tcgggccgag cagcgcaaag aaatcgccgc
     7621 gttcgacctg cagatcgacc cctttgaggg cctggacgcc gttgtcgtag gtcttgacga
     7681 gcccgcgggc ggaaagcgca tacatggggg cgcgaagcct accatatggt ccgccggccc
     7741 ggtccgaccg ggctcagcgc aggcgggggc gccggggatc ggccggccac cagggcgcgg
     7801 ccagcagctg catgccgcgc aggcgcgcga gttcgaattc cgccggtgcg gcgctggccg
     7861 cggccgagag caggtggcgc cagatttccg ggtgcgcgag ccagcgcgga cgcagcagcg
     7921 cgggcgcgat ctcgatgcag gcaccgagtt cgcgcagcgt gaggcccgcg agccaggcac
     7981 tgtcggcctc gtgcaggccg agcacgatcc gcgccgccag gggtcgactt cgcgcgatgt
     8041 gccacgcgta agtcagcgta agataagcaa gtcgcggcag ggattgctcc tcgaaccagt
     8101 gctcgccctc gccgctgtcg cgcaccccgc cgcccgcact gagcgcagcc acgtcgatct
     8161 cgaccagcag gaacgggcag ccggcgatcc tgcggccctg ctcgggcgtg agcgcgcgcc
     8221 acaactcgac cgaatcgcgc aggacggcgt gcagccccgc cggcacgccc gccgcgcgcc
     8281 gggtcatcag ctcgagcgcc agttcgttga tctcggcgag cggcgcgagc gccgtggcgg
     8341 atggccctgt atccacgttt ccctccgatc gcggccgata ttgcgcgcat ttggggcttc
     8401 ccggccatac ccaaatttgg gatatttcaa ccaaatcggg cctggcacca ttcagggagc
     8461 gaaccgccat gcgcatctcc gatgaccgct acagccgcga tcggctgcgc ttcgatctcg
     8521 cgctgcgcct gatcgggcac gaggcacgca cccacacgat ccgcgcctgg accgggctca
     8581 ccgaggatcg cgtgcgcaag ctctatcgca cctacatcgc cgagcatccc ggccagaagg
     8641 tggcgcgccg tcgcggcaag tcgccgcagc gctgcagcta tttcctgcgc acgccgcgcg
     8701 tgcagcagga agcggccgtg ctggccggcc tgtattgcat ggtcggcatc attgcgcagg
     8761 gcgcgcgcct cgatccgcgc atgctctcgg tcgcgcaggg cgagttgctg tgcgtcgcgt
     8821 acgaaaccta cctcgcactg gtgaagtccc cgcagatctc gttcgagcac gccgcgttcc
     8881 tcgcgatcgc gctggcgcgc ggcgatgagc tcgcgctgcg ccactgcggc agttgcgggg
     8941 cgctcgcgct cgccgaccgc tatacgctgc gcgccaccgt ttgcgcggta tgcgccgaat
     9001 cggcagcgcc gctgtcggcg ctgcgcatcg atggccgccg cgcaccgccc gctgccgcgc
     9061 gcggcacacc cgcgccggcc cggctcgggc cggtcgatac gggctgagcg cacaccttat
     9121 ataatggccc gcagcccggg cgggccccat gcagcagcag cgcaagcact tcaatcattt
     9181 cgccggccct tatctcgacc gcaacgcgtg ggaacgcgag gacgcgggtt ggctcgcgac
     9241 cgcgcggcgc gacagctcga cccgctatct catcgcacgc ggcaccgccc acctcgtcgt
     9301 gcacgagccg gcaccgcaca tcgtgttcct gggcggcgac catccgcgca tcgcgctggc
     9361 gcaggagcgc cagaccgtac tgctcggctg gttcgagggc gcgcgttgcg tgctggtcga
     9421 ggacgatgcg gacggagaca tcgcgccgcc cggtgcccgg ttcgaggaac tgcggccgct
     9481 gtcggccacg ctgcccgagc gtgaggcggc gctgatcgcc tacgcgcgcg cgctgtcgat
     9541 ctggcgggcg cgccaccgct actgcggcgc ctgtggcgcg ccgacccggc cactgcgcgc
     9601 cggtcacgtg ctcggctgct cgaatccgga ttgcggccag caactgttcc cgcgcatcga
     9661 tcccgccgtc atcgtgctgg tgacagatgg cgatcgcgcg ctgctcggcc gtcaggccag
     9721 ttggccgaag aatcgatact cgacgatcgc cggcttcgtc gaaccgggcg agagcatcga
     9781 agacgccgtc gcgcgcgaag tgctggagga aaccggcatc gcggtgggcg aggtgaccta
     9841 ccactcctcg cagccctggc cgttcccctc ctcgctgatg ctcggcttcc atgcccgggc
     9901 cgagggcggg gaaatcgtct gccgcgacgg cgaactcgag gacgcgcagt ggttctcgcg
     9961 cgccgagatc gccgcgggcc atccgctgct ctcgccgcgc acctcgatct cctggcgact
    10021 catcgagtcc tggttcgatg ccggctggca cacgcctctc gcacggatcg gctgatggag
    10081 atctccacgg cgagcgccct gctctcggtc gccctcggca tcggtcttgc cgcggccgtc
    10141 ggcctgcggg tgttcctgcc gctgctgatc accgcggtcg cgggcgctac cggccatgtc
    10201 gacctcgcgc cgtcgttcga gtggctggcg acgaccccgg cgctggtgat gctcggcgtc
    10261 gccaccgtgc tcgaactgtt cgcgtactac attccgggcg tcgacaacct gctcgacgtg
    10321 atcgccacgc ccacggcggt cgtcgccggc gccgtcgtgg cagcggcggt catggccgac
    10381 ctgccgccgc tcgtgaaatg gaccacggcg atcatcgccg gtggcggagt cgccgggctg
    10441 acacagggtg tcacggcact cgtccgcgcg aagtcaaccg cgacgaccgg cggcctcggc
    10501 aacagcgcgg tcgcgaccgg cgaactcggt ggctcgatcg tgctcaccgt gctcgcgctc
    10561 gccgcgccgg tgatcgccgt cgcactggtc gtgctcgtcg tctggctcgc agtgcggctc
    10621 gtgcgccgac tgctgcgccc ggtgcgctga ggccgcggcc gtgtgcctgc tggtgctcgc
    10681 gtggcgcgcg catccgcgct atcggctggt cgtcgccgcc aatcgcgacg aataccatgg
    10741 ccgccccgca gcggcgatgg gtccgtggaa cgacttgccg atcctcgccg gccgcgacct
    10801 cgaggcgggc ggcacctggc tcggtgtcga tcgcgcgcgc cgcttcggcg tggtgacgaa
    10861 cttccgcgag atggtgcgtc gtccggcggg ggcgccctcg cggggcggcc tggttcccgc
    10921 atggctcaag gacacccgcg gcgtcgccga tcacgcggcg agcctgcgcg cgaccgcagc
    10981 cgactacgcg ggcttcaacc tgttgttcgc cgatcgcgac tcgctgtact acctgtcgaa
    11041 ccggctcgag tccttttccc gacccctggc aagcggcgtc catggactcg ccaaccacta
    11101 cctcgacacg ccctggccga agcttctgcg cgtgcgtcac ggcctcgaac gctggctcgg
    11161 cgagcgcaat gcggacccgc gctcgctgtt cgcgctgctc gccgatcgcg cgcccgccag
    11221 cgacggcgac ctgcccgaca ccgggctctc cccggaatgg gagcgcgcgc tctcggcccc
    11281 gttcgtcgtc catggcgagt acggcacgcg ctgctcgacc gtgctggcgc tgggccatga
    11341 cgacagcctg cagatcgagg agcaatcctg ggacgcaacc ggcaggctca ccgacgtcgc
    11401 ctcctacagg ctgggtccgg aggactggcc cgcagggaac ccgcccgcac cagcgcaact
    11461 ctgattaaac tccgccaatt tactttttca cggacgagat gccaggaccc cagcgcgaca
    11521 cccggtcggc cacggcgcga tgcctgctcg cgctgtgcgc gctcgtcgcg acgtcggccc
    11581 acgccgacat cgacatcgtc atcgagggcg tcgagggtcc cgtgcgccgc aacgtcctcg
    11641 ccttcctctc cctcgagcgc tacaaggatc gcgatcggct tcgagaggac acgatcgagc
    11701 ggctgcacga acgcgccgag cgcgaggtgc gcgccgcgct gcgcccgttc ggctattacg
    11761 agccgaccgt ccgctcgagc gtggaggcgc gtcccaatgg ccaggactgg cgcatcgaga
    11821 tccacatcga acccgggccg ccggtgatcc tggaaaccgt cgaggtacgg gtcaccggcg
    11881 cgcgcgccaa cgacccggtg ttcaccaatc tgcttgccgg catgccgctg cgggcggggg
    11941 cgcaactgca tcacagcgac tacgagcagg tgaagagcga cctgcagcgc accgcggcgg
    12001 cgtacggcta cctcgatgcg cgcctgctgc gccacgagct gctggtcgac ccggcaacgc
    12061 gcaccgcgag cgcccacatc gagttcgaga ccggcgagcg ctaccggttc gggcccacgg
    12121 agatcgcgca ggatgtcatc cggcccgaac tgatgcgccg cttcctgcgc tacgaggaag
    12181 ggcagccgtt cgacgccggc gaactgctcc gcacgcagtt cgcgctcgac gacagcgcct
    12241 acttcgcctc ggtcgaagtg ctgccgcagg agcgcgatcc ggcaacgttc acggtgccga
    12301 tacgcatcgc cgcgaccccg aaccggcagt cgcgctggac attcggcatc ggctacggca
    12361 ccgacaccga ggcgcgcggc acgatcggct gggacaaccg catcgtcaat gcactcggcc
    12421 accgcttccg cgccgaagtg aagctcgcgg agatctcgca gcgggtttcc ggccgctatg
    12481 tctggccgat cggcgacccc gcgctggaga agttggaggt cgaaggcacc tgggagcacc
    12541 agaccctagc cgacctcgac acctacaaca ccgaagcacg cgccggcata acccgcgtgc
    12601 cgcatcgctg gcagcgcgtg tactacacgc gcctcgtgcg cgcgatcacc gaggacgcca
    12661 cttcgcgcgt cgagtccctg ctcctcattc ccggcttcag cgtcgcctcg gtaccgaagg
    12721 gatatctcgg cgagccgctg ttcggccgcc agctctacgc cgagctgcgc ggctcgcaca
    12781 cagggctcgg cgcggatacc gactacctgc agctgctcgc ggcctccgag cacgtgttct
    12841 cgatcgcgcc tgcgtggcac ctgctgctgc gcgcggaaat cggcacgacc tgggccgacg
    12901 acttcgacgg ggtccccggg tcggagcgct tcttcgccgg cggcgaccgc agcgtgcgcg
    12961 gcttcggctt caatgacctg tcgccggtgg acgagaacgg cgagaagacc ggcggcaagc
    13021 acatggtcgc cggcaccgtc gaggtgattc gtgacctgcc gcgcaacctc ggcctcgccg
    13081 tgttcgtcga tgcgggcaac gccttcaacg ccgtcagcga ggggctcgag tattcggccg
    13141 gcatcggcct gcgcttccgt ctgccgatcg cgaccgtggg cttcgacatc gcgcaggcac
    13201 tgtccgtcag cggtgccacg ccccgcctgc acgtcaactt ctcgccgaag ctctgatggc
    13261 gcgcccagtc agcatcctgc tggtgctcgc gatcgccctg ctgtgcgcgc tgccatttgc
    13321 gctcatctat ttcatcgcgt tcacgccgca gggactcgcg atgatcgtcg cgcggatgcc
    13381 cgagcgcgtc ggccccgtga gtctcgacat cggcacggtc agcggcacga tcgccggcgg
    13441 cttcgagctg tcgcgcttcg agctcgacca tgagcgcgtg cacctcgtcg ccgaggacat
    13501 ccaggcacgg ctcgcggtgg cgcccctgct gtggcagaag atcgaagtgc ccgaagtcca
    13561 cacgcggaac ctgctcatag aagtgaagac gcgaccgcag cgtccgcgca agggcacgcc
    13621 caggttcctc ccgcggctgc tgcgcatcga gactgacgac gcgcgcgccg cgctgctcac
    13681 gctggtgtcg accaagggtg tccgcaccga gttcgccgac atccgcgcga gcggcgtcgt
    13741 gcgtcaccgc tcgatccgcg tgttcggcgc tgccgcccgc ttccagggca tggacctcac
    13801 ggccaacggc acggtgcgcg ccgccagtcc gatgcggatc gatgcgagcg cccgtgtgga
    13861 atggttcgcg ccgaagtcca cccgctggat cgcgcacgtt gccggcgatg gcgacctcga
    13921 cagcctgccg ttcaccggca gcatcgaggc accgttccgt gccgcggtga gtggcgccgc
    13981 agaaaccctg acgcgcgcct ggaaaatcgt cggcagcgca cagataagcc agctcgatat
    14041 cgccgacttc ggcggcggcg gggcgctcgg tgaaatcgac gggcgggtca aggtcggcat
    14101 caccgccgat ggcatcgccg cggatggcgt gatgcatccc gcaggtctcg aagccggcga
    14161 tttcgacgtg cagttcaacg gcaggtacgc ggcgagaaca ctgcaggtgc ggcacgcgca
    14221 gttccggcac cggggttcgg gcgcgctcgc gactaccgcc ggcgagatcc gcatcgacga
    14281 ggccggccca tcgctcgacc tcgcgggtga gtggcgcgaa tttcgctggc cgcttgccgc
    14341 ggccgagccc gccgtccaca gcgcgcaggg cagttaccag ctggcaggcg ccatgcccta
    14401 tgcgatcacc gcgagcggcg ccgtcgtcgc ggcggacctc gagccgatgc aggtcgtgct
    14461 cgaaggccgg cttgcgggag accggctcga tgccgacgtg ctgcgggtcg agggatacga
    14521 aggccgcgcg gacctgcgcg gcgccgtgca gtggcagccg tcggaagcct ggcaggtggc
    14581 cggccgcatc gccggtgtga acacgggtcg ggtcaacggg cgtctgcccg gtcgcgtcag
    14641 cctggacatc gacgcgcgcg gcgagggctt ctcgcccgag ggcagcctcg atctgcgatt
    14701 tgcacgcctc ggcggcaagg tgcgcggcgg cagcgcgagc ggtgccggtc gcatcatccg
    14761 ccgtggtgcc atgctgggct tccacgactt cgacttgcgt cttggcgcgg cacgcgcaca
    14821 gctcgaaggc gatatttcac cggacgcgta ctcgctgcgc ttcgacttcg atgcggacga
    14881 cctcgccctg ctcgacgccg gcgccaccgg caggctggcc gcgcgcggca cggtgggcgg
    14941 atcgcgcgcc gcgcctgcga tcgacgcgac gctgcggggc ctggccatcg actacgcagg
    15001 cgtgcaggtc gacggtatcg agggccgggt ccgcttcgac ggccgcgccg gacaggcgct
    15061 ggacatcgac ctgcaggcca ggggaatcac gcggggggcg cgcaagatcg agcgcttcac
    15121 cgtcaccgca aagggcacga ccgagcagca cgaggtggcg atgaccacga acgcggcgcc
    15181 cgtcgcaatc ggcgcgcgcg gacccggcag ttatcgggcg ggcacctggc agctgcagtt
    15241 ccgggagctc gacctggaaa acggcgacga ggcggaactg cacctgtccg cgccatccct
    15301 gctcacggtc tcgcgcgact cgcaacgact cgagacgacc tgcctgcgcg gcacggttgc
    15361 ggagctgtgc gcgaccggca accacgcccc gcaggagtgg gccgtgaccc tgtccgcgaa
    15421 acggttgccg atcagcacct tcacggccgg ccttacaccg ggcgtgacct acgagggctc
    15481 ggtcgacatc gaggccgccg cgagcgcgcg cgacaacgaa ccctggcgcg gcagcctgcg
    15541 tgcggacctg ctcgacgtgc agacgcggcg gcggctcgcg aacaaccgtg tcgacgtcac
    15601 ggtaatcggc ggcggcgtca tcaccggatc ggccaccgcc gagtcgatcg tcgtcgacgc
    15661 cagactcgat gccggagcct ccggcgatct gaccggcaat gcgcagatcg cgcgcagtgc
    15721 cgaggactgg cgcgactggc cgctgcaggg ccggctcgat gcgcgcaccg attcgctcgg
    15781 cttcattgcc gtgctggtcg gcacgatcga ccgctcgtcc ggcagcctct cgaccaacct
    15841 gcgcctctcc ggcacgctcg gtgcgccgcg attcgagggt ctgctctcga tccgcgacgg
    15901 gcaattcgat atctaccagg tgaacctccc gctgcgcgcg gtcactctcg atgcgcactt
    15961 cctcaaggac acgctgacac tcgagagcac ggcgaccgcc ggcgacggca gcctggccgc
    16021 gggcggcgcg ctgacctggc gcaagggcct gccatacggc aagctcacga tgcgcggcga
    16081 gaacctgcgc atcgcgaatg tccccgaagc gcgcatcttc gcctcgcccg acctggtgtt
    16141 cgacgtcgac gggcgccata tcgatgtcac cggcgcggta ctcgtgccga atgcgcgcct
    16201 cgacccggcg gatatcaccg gggcggtgtt ttcgtccgcg gacgagcgca tcgtgggcga
    16261 gcccgtggtc gatccgtcca agcgcttcac cgtgacgacg cgcgtgaaca tcgcgctcgg
    16321 tgatcgcgtc acgatcgata cctccggcct ttccggccgg ctcaccggcg cgctcgaagc
    16381 ccgcactgac gtagacgacg tggcgcgcgg caccggcgaa ctcgcggtcg ccgagggtcg
    16441 ctacctggcg ttcggccgac ggctcgacat cgaacacggc aggctgatct tcaacaacgg
    16501 gccgctcgcc gaccccggca tcgacatccg cgccagccgc gaattcccgg atatcagggc
    16561 cggcgccaac atacgcggca cgctgcgcgc gccgcgactg acactgttct cggagccgca
    16621 gatcccgcaa tcacagatac tctcgctgct gatcgccggt ggctcgctcg agtcggcgca
    16681 ggcggcggag ggcgggcgca ccagcaatcc gtttgccgtg cagggcggcg ccattctcgc
    16741 gcagcagctc ggccagatcg tcggcctcga ggacgtgagc ctcgagtcgt cgctcaacaa
    16801 cgagacctcg ctggtgctcg gccgctatct cacgccgcga ctgttcgtca gctacggcat
    16861 cagcctcacc gaagcgatca acaccatcaa gatgcgttac accctgggcg acgactggac
    16921 ggtcaagctc gaatcgggtg ccgtgcagag cgcggatctc cagtacacga tcgagcgctg
    16981 agccctgtcg ccggcacgcg cggccggccc ttgaccatgt ccgcatcttt atgcataatc
    17041 tctgcaagat tatgcatacc tcgccggaac aagccgccca ccgccgcgac acgatcaccc
    17101 gcctgatccg cagcgggacc gtgcgcaagc aggccgacct cgtgcgcctg ctgaaggccg
    17161 agggccacgc ggtgacgcag tcctcgatca gccgggactt gcgcgaaatc ggcgtgctga
    17221 aggcggccga cggctatgtg ctgcccgccg gcgatgcccg cccggccggc gacttcaccg
    17281 cgcttgcgca gttcgtgcgc ggcctgcgtt tcgcgggccc ggcgctcacg gtgctgcgca
    17341 cctcggtcgg cgccgcgcag agcgtcgcgg ttgcgatcga ccaggcgaac tggcccgagg
    17401 tcgcgggcac gatttccggt gacgacacga tcttcatcgc gacggccggc gcgcgcgacc
    17461 aggccgcact cgccaggcgt ctgcgttcct tcttccccat ctgatcccga gaccatgacc
    17521 caacctatcg tcctcgccta ttcgggcgga ctcgacacct cattcctcgt accctggctg
    17581 gccgaacagt acggccgccc ggtgatcacg gtcaccgtgg acaccggcgg catcgatgcc
    17641 gccgccgcgc gcaccctcga ggagcgcgcg ctggcgctcg gcgcagttgc gcaccaccag
    17701 gtcgacgcgc gtgccgacta cttcgagcag gtgctgcgct tcctggtcat gggcaacgtg
    17761 cgccgcggcc agctttaccc gctgtgcgtc ggcgccgagc gcgtcatgca ggcgcagacg
    17821 atcgcccgca tggccgaaaa gctcggtacg cgcagcatcg cgcacggctg caccgcggcc
    17881 ggcaacgacc aggtgcgctt cgaggtcgcg ctgcgcacgc tcgcgcccga gctcgagatc
    17941 atcgcgccgg ttcgcgaccg ggccttccag cgccaggagg agctcgacta cctgcgcgcg
    18001 cgcggcctgc cggtgccgcc gttcggcgcc gcctattcga tcaaccgcgg cctgtggggc
    18061 gtgacgatcg gcggcaggga gacgctcact tcggagggca gcatcccgga ggagcaatgg
    18121 gtgctctcga aggatgcctt taccaacccg cgcgcaccgc agcgccacac gatcgggttt
    18181 gcggctggcc gtccgatcag cgtcgacggg cacgaactct cgcccgtcgc ggcgatcgag
    18241 accctcgagg cgctcgccgc gccattcggc attggccgcg gcatccacct cggcgacacg
    18301 atcatcggca ccaagggccg ggtcgccttc gaagcgcccg ccgccgaggt gctgctcgtg
    18361 gcccatcgcg aactcgagaa gctggtgctg accgcgcgcc agcagcgcat caaggaatcg
    18421 gttgcgcagc cctacggcga tctggtgcac gaaggccagt tgctcgaccc ggtatgccgg
    18481 gacatcgagg cgctgttcct gtcgtcacag gagcgcgtga ccggcacggt ctccgtgctg
    18541 ctgcgtccgg gcagcgtctt cgtcgaaggc gtgagctcgc cgtggtcgct gatggccgcg
    18601 tcgaagggcg tttatggcga gtcggtcggg gaatggtccg cggccgacgc gctcggtttc
    18661 tccaagctcg tcgcactgcc gggcgtgttc cacacgcgcg ccggcaagct cgccgggagt
    18721 gcccgctgat gatgaagtcc gtggtcgtcg acaagatcgc ctcgatcacg caggccgcgg
    18781 gcctgtcgcg cgagctgcgt atctcgaccg acatcccgtc cgaggagggc gtgatcgtcg
    18841 tcgtcgagat cctgacgaac aagtcgacct acaacacgct cgaactgacg agcgggcgca
    18901 tggcgaagct cggcaagggc gacatcgtcg tcggtgcgct cggtcatcgc aaggcgctgt
    18961 tcggctactc gggccacgtg ccggaatccc tcaaggccgg cgatgtgatc cagatgctca
    19021 acatcggcgg cgtgctcggc gtgtgcgatt cggtcaatcc cgacaagggc acgccgttcg
    19081 actgtcgcgt actcggcgtc gtcctgcagt tcccctacct cggcgagcgc atcggcgtgc
    19141 cggcgcgcgc aggctaccgc aacctcgact acgccacggc gctcgacacg cgcggtgtgc
    19201 cggtggtcgc gctcgccggt acctgcatgg aagccggcaa gaccgccgcg gcgtgttcga
    19261 tcgtgagccg catgcgccat cgcggcctcg ttgtcgacgc gttcaaggcg accggcgtct
    19321 cgctccgccg cgacatcctc gcgatggcgg atgccggcgc gcgcaatacc atgatcttca
    19381 ccgacctcgg cgtcatcacg accacgccga agagcggacc ggccctcacg cgcacgatgc
    19441 tcaccgagct cgcggcaacc aggcccgacg tgatcgtgtt cgagctcggc gacggcctga
    19501 tgggcaccta cggcgtcgac gcgatcctct cctgcgacga catcagaacg gcgctcaccg
    19561 gcgtggtgct gtccgccaac gatccggtcg cggcatgggg cggcgtcaag atgctgcgcg
    19621 agcgcttcgg catcgaaccc tgtgtcgtca cgggcccggc caccgacaac gcggtcggcg
    19681 tcgacatcat ccgccagcag atgaatgtcg cggcgttcaa tgcgctcagc tccggcgccg
    19741 cgctcggcga ccacatcatc gaggccatcg gcttgcacgc gaagcccgtg gaagcgctcg
    19801 cgggatgagc ggcgggcgca taccggcgat cgtgctcggc ggcaccggct atgtgtcggg
    19861 cgagctgctg cgcctgctcg ccgcgcatcc acatttcgcg ctcgcgggag tactgtccga
    19921 cagccagccc ggcgaggcgg tcgccaaagc gtttcgccat ctcgcgccgg cgctcggcga
    19981 cctgagcttc ggcacgcagg ccgaaatcga atcgctgatc ggcacgctgc cgcgcctggc
    20041 actcttcggc gccgcgccgc atggcgtttc ggctgcgctg atcgatgcgc tgctgacgcg
    20101 cgcggaggcc gccgggtccg acgtgcacgt ggtcgatatc tccgccgatt tccgctaccg
    20161 ggatgccgcc gcctacgagg ccgtatacaa gcacccgcat ggcgcgccgg gacgcctcgc
    20221 gcagttcgcc tgcgccgtgc ccgagcatct cgcgcgcgcg ccgacgcgcc acgtggcaca
    20281 ccccggttgc ttctcgaccg ccacgctcct tgcgagcgtg ccgctgctcg cgctcgatct
    20341 cgtgacgccg acgctgttcg tcgccggcgt gaccggcagt acgggctcgg gccgcaagcc
    20401 ctccgagggc acgcaccatc cgcagcgcca cggcgatttc tatacctaca atgcgctggc
    20461 gcaccggcac acgcccgaag tggccgcctg cgcgcaggcc gcgagcgggg tcgccgccga
    20521 cttcgcattc gtgccgcact ccgggccttt tgcacgcggc atccatgtca cggtgcaggc
    20581 gagcctgaag gcgcgccccg gtgcggccgc acccgatacc gcggcggtgc tggcggcgct
    20641 gcgcgatttc tatcgcggcg cgccgttcgt gcaggttgcg gcggaggcgc cgcacgtcaa
    20701 ggacgtcgcg gcgagcaact acgcgcgcct gtcggcggcg gcgaacggcc gcacggtcgc
    20761 ggtcacctgc gtggtcgaca acctgaacaa gggcgcggct ggcggcgcgg tgcagtggat
    20821 gaaccgcctg ttcgcgctcc ccgaggacgc gggcctcacg gcgcccgcgg cgggatggac
    20881 atgagcgccc tcgcacaggt ctttgcccag tatccgctcg aggtcgagca cgccgaaggc
    20941 gtgtggctct actcgcgcga cggtcggcgc gtgctcgacc tgtatggcgg ccacgcggtc
    21001 gcggcactcg gctacggcca tgccggctgg acccgcgcgc tgcaggcgca ggccgcgtcc
    21061 tgcaatttcc agagcaatgc ggtgccgatg gcggtgcgcg agcgtgcggc cgtgcggctg
    21121 ctgcgcttcg ccggcggcca cttcgacagc gtgttcttcg tcaacagcgg cgcggaagcc
    21181 aacgagaacg cgctgaagat ggcctttcgc atgaccggcc gaccgcaggt cgccgcggtc
    21241 gaaggcagct tccacgggcg caccgcggcg gccggcgcga tcacctgggg cgcgatgggg
    21301 aagtggcatg cctttccccg tgcgcccttc gacgtgtcgt ggatcccgcg acgcgagagc
    21361 ggcgcggtcg cgcagcacgt caccgaagac acggcggccg tcatcgtcga gcccgtgcag
    21421 ggtgtcggtg gcgccttcga catgggcagt gacttcctcg gtgcgctgcg ccggcgctgc
    21481 gacgaagtcg gcgcattgct gatcttcgac gaagtgcaat gcggcgtggg ccgcacgggg
    21541 tacgcgttcg ccgcggacct ctacggcgtg cggcccgaca tgatcacgac cgccaaggcg
    21601 ctcggcaacg gcttcccgtg cgcggcgctg ctgatgacac cgcgcgtctc gcaggccgtg
    21661 acgctcgatg cgctcggcac gaccttcggc ggcggtccga tggcgggcgc ggtcatcgag
    21721 gcggtgatcg aggcgatcga atcggaagag ctgctcgcga acgtacgccg cgtgtccgcg
    21781 tatatccgca cgcgctgcgc cgtaggcccg gtcaccggct tccagggcga gggcttcctg
    21841 ctcggactgc gcatgaaccg ccccgcccgg gacatccagg cggcgctgct cgcgcgcgac
    21901 atcctcaccg gcacgagcgg cgacccgcag gtgctgcggc tgctgccgcc gtacgtgctc
    21961 gaggagcagc acgtggacct gctggcggag gcgctcgcgt cgctgtgacg cgcaacccgg
    22021 acatgcaacg cttcctcgac cttgcagact tcagccgtga cgagatcgtc tcgttgctcg
    22081 cgctcgcgcg ccgcctcgag gcgcatcccg aaccgcaagc gctcgcgggc cgcatcctcg
    22141 gactcgtgtt cttcaatccg tcgctgcgca cgctcgcctc gttccaagcc gggatggcgc
    22201 gcctcggcgg cagctcgttc gtgatcacgc ccggccaggg cacctggcag ctcgaaacgc
    22261 gcctcggcgc cgtcatgaac ggcgccgccg cggagcatgt gcgcgagggc ctgccggtgc
    22321 ttgcctcgta ctgcgatgcg ctcggcatcc gcgcgttcgc ggaaggcaag gacctcgcgg
    22381 acgatatcgc cgagagccgg ttcggcgcga tggcctcggt catcgacaag ccgctgatca
    22441 acctcgagtc cgcgatgaac catccgtgcc aggcgctcgc cgactggaag accatggacg
    22501 atctggcgat cccgcggcag gcaaagttcg tgctgtcgtg ggtgcagcat ccccgcgcgc
    22561 tgccgctcgc ggtgcccgcg gccaccgtgc acatggcggc gatgcgcggc atggaagtcg
    22621 tggtgctgcg gcccgagggc tatgcgctgc cgccgcaagt gatggcgaag gcacgcgccg
    22681 cagcggccgc ctccggtggc tcggtgcgcg agaccagcga gcgcggcgag gcgctggccg
    22741 gcgcccaggt gctgtacgcg aaggaatggg gctcgaccgc ccactacggt gacgccgagg
    22801 gcgatgcgct gctgcgcgcg cagctcaccg actggtgcgt cacgcccgaa tggttcgagc
    22861 cggcggatcc gcaatgccgg ctcatgcact gcctgcccgt gcgtcgcaat gtcgcgatcg
    22921 ccgacaccgt gctcgatggc ccgcgcagca tcgtgcggca cgaggcctac aatcgcatga
    22981 ctgcgcagat ggcggtcctg catcgaatgc tttcccaggg agtccggaga ccgacatgat
    23041 gaagcaccgt gccgaccagg cactcgccgt ccgctcgctg cgcagcgccg ccccgtacat
    23101 ccgcatgtac aagggcaaga ccttcgtgat caaggcgggc ggcgccgtgt tcggcgatgc
    23161 gcaggccacg cgtgcgctga tcgagcagat cgcgatcctg cattatttcg gcgtgcgcgt
    23221 cgtgttcgtg cacggtggtg gaccgcagct cagcgagctc accgaggcgc tcggcgtgcc
    23281 gacccgcatg gtgcagggcc ggcgcatcac ggaccagaag tcgatcgacg ccacctcgat
    23341 ggtgctgaac gggctgatca acacccggat actcgccatc tgccgcgagc tcgacatcag
    23401 cgccgtcggc gtcagcggcg tcgacgcctc cctcgtcact gcgcaccgcc gcccggtcgt
    23461 cagaatgccc gatggcgaga acgtcgactt cggcttcgtc ggcgacatcg acgcggtcga
    23521 caccacggtg ctgcgcaagc tgctcgacaa cgggctgatg ccggtcgtga gcccgctgtc
    23581 ggccgacgcc ggcggcgtgc tgctcaacat caacgccgac accgtcgccg cggcgatcgg
    23641 cgcggcgctc ggcgccgaga agctgatcct gtgcacgggc gcgcccggca tcctcgagcg
    23701 tgtcgaggac ccgacgtcgg tcgtctcgta caccgacctc aacggcctca agcgcatgcg
    23761 cgaggacggc agcatcaagg acggcatgct gccgaaggcg aaggcgatcg aggacgcgat
    23821 ccgtggcggc gtgcggcgag tgcacgtgat cgcctatacc tcgcccgaga gcatcctcgc
    23881 cgaagtgttc accaacgagg gcacgggcac gctgatcgtc gccgatatca atgcgctcag
    23941 cgtcgccgaa cagcagagtg gagccgcgtg acgcgcctgt gggacaaggg cgcaccgctc
    24001 gatgagcgcg tgctcgccta cacggccggc gaggactacg cgctcgatga gcgtctggtg
    24061 cgctatgacg ttgcggcctc gatcgcgcac gctgcgatgc tgcatgcgca gaagctgctc
    24121 accgacgccg atttcgtcgc catccgcgag ggcctcaccg ccatcggcga ggcgcatgag
    24181 cgcggcgagt ggcgcatcga actcgccgac gaggatgggc agagcgcgct tgagcggcgc
    24241 ctcacggcgc gcatcggcgc ggcgggtggc cgcgtgcacc tcgggcgctc gcgcaatgac
    24301 caggtcctga cggcgctgcg actgtacctg cgcgatgcga tagagcagct gcgcacgggc
    24361 gcctcgcagg tcgccgatgc actcgatgcg ttggccgcgc gcggtggtga aatcgccctg
    24421 cccggctaca cccacatgca gcaggcgatg ccgagcaccg tcgcgttgtg ggccggcgga
    24481 ttcgccgccg agctgcgcga cgacgccgag ggactgcgtc tcgcgcatcg gcgcaccgcg
    24541 aagtgcccgc tgggttccgc tgccggctac ggcacgcccg gtctgccgct cgatcgggac
    24601 gcgacccgcg cggcgctcgg cttcgcggtg acgcacgagc ctgtcactgc cgtgcagctc
    24661 tcgcgcggca aggcggagac gcagctgctg ttcgaggtcg cgctcacgat gcaggacctc
    24721 ggccggcttg cggccgacct gttgctcttc tatacgcagg agttcgcctt cgtcaccttg
    24781 ccggacgagt tcacgaccgg ctcgtcgatc atgccgcaga agcgcaaccc ggatgtcttc
    24841 gagctggtgc gcggccgcac cgcgacggcg cacgccaacc tcgtcgaggc cctcggaata
    24901 ttcgccaagc tgccgtcggg ctaccagcgc gacctgcagc tgctgaagct gccgctgttt
    24961 cgcggcatcg atatcgcgct gcagacgctc gacatcatgg ccgccgccat cggcgcggtc
    25021 cacttccgcc gggagaacat cgcgctcgac cctgcgatcc acgcggccga ggaagcgaac
    25081 gcgctcgtcg tgaaggaagg catcccgttt cgcgaggctt atcgccgcgt cagcgcgcgc
    25141 ctgcgcaagg tttgatccgc ttcgggggta accatgtcga tctggttccg gcaacccacg
    25201 cgcgaacagc tgaatgcgct gtcggccaat acgctcgtgg cgcatctttc gatcgagttc
    25261 accgagatcg gcgacgactt catgcgcgcc acgatgcccg tcgatgcgcg cacgcaccag
    25321 ccgttcggcg tgctgcacgg tggcgcgtcg gtggcgctcg ccgagactct ggcgagcacc
    25381 gcggcgagct gctgcatcga cacggctcgc ttcatgtgcc tcgggcagga gatcaacgcc
    25441 aaccacctgc ggcccgtgcg tagcggcctc gtcaccggca ccacccgccc gtatcacatc
    25501 ggggcgcgca gccaggtgtg gggcattgag ctgcgcgacg ccgcgggcca tctcgcctgc
    25561 gtctcgcgca tcaccatggc gaccgtggag cgccggcagg tatgatccgg cagattgcag
    25621 gaggctcagc ccatgaatga agccaaccag cccaccgccg gtctcgatcc cggcctcgtc
    25681 acctatacga acatcatcta cgggctgcac gcgctgtccg tgctgatcgg cgtgaccggc
    25741 cccgcgacgg tgatcggcag tttcgtcttc ggcctgccgt cgatcatcgc cgtgatcatg
    25801 aattacgtgc gccagtccga agtgcgcggc acctggctcg aatcgcactt ccgctggcag
    25861 atccgcactt tctggttcgc attcctgtgg atcgtcgtcg ccgccatcgt gtcggcaccg
    25921 ctcgtgctgc tgtttggact tggaatcgtc actttctacc tggcggcagt gatcatcgcg
    25981 atctggatcg tctaccgagt cgcgcgcgga tggatcgcgc tgcgcgacaa acgcacgatg
    26041 ttcctgtgat gtgccgcgcc ctggccgcgg caactctggc gctcgcactg ctcgcggcat
    26101 gcgggcagaa gggcccgctc tacctgcctg accagggtgg caatgtcgtg gtgcgcccgg
    26161 cggcggaacc gacgccggca ccggcacccg tcgacgacga tgcggatccg aaaagcggca
    26221 ggaaaccgcc cgaggccgcg cccgcgccct gagcgactac cctcagaccg gctttccgcg
    26281 cagcactcgc gccggcagca ggatgatgga cttcaacagc tcgaacacac cgttcacggc
    26341 gatgccgaga agccggaacg gcagcagcac cagccacacg agcgggtaga gcagcaaccc
    26401 gatcagcgcg atcggccagc agatcacgag cagcagtagc cagaacagaa acgcgagcat
    26461 cggtgtcctc cctggagaac gcagtataca agggcttgat cctgggcttg agtccagagt
    26521 gcatacttga ggcatgcaga tcggtgaact cgcgaaatcg gccggcgtgc ccatcgacac
    26581 ggtgcgttac tacgaacgcc agggcatcct gcctgcgccg cggcatgggg gcgtgaagaa
    26641 ggccgcgacg gcgaagctgg ccgcgctttc cggtgaggaa tcatgaccca cgaacaccac
    26701 gagcacgcac accactcgca cgaaggtcgc gtgaccgacc ccgtgtgcgg catgaaggtc
    26761 gatccgcaga agacgccaca ccatgccagc catggcggcg cggcatatta cttctgcggc
    26821 gcgcgctgcc gcgaacgctt catcgccgat cccgggaagt atcttgcggc acaggcgacc
    26881 gggatgccgc cggaatcgcc cgccgcgccc gccggcacga tctacacctg cccgatgcat
    26941 cccgaggtgc ggcaggaagg cccgggcatc tgcccgaaat gcggcatggc gctcgaaccg
    27001 gagatgccga gcctcgacga cgaggacaac ccggagctgc gggatttcac gcagcgattc
    27061 tggtggtcac tgccgctgtc ggccgtcgtg ttcgtgctcg cgatgttcgg ccaccatgtc
    27121 ccggggctgt cgatcggcgc gcgcacctgg atcgagttcg cgtgcgcgac gccggtcgta
    27181 ctgtgggccg gccggccctt cctcgagcgc tggtggcagt cgctgcgcaa tcgcagcccg
    27241 aacatgtgga cattgatcgg cagcggcgtg attgcggcgt acggctacag cgtcgtcgcg
    27301 acgcttgcgc cgggcatctt cccggactcg ttccgcgagc atggtcgcgt gggcgtgtac
    27361 ttcgaggccg cggcggtgat catctcgctg acactgctcg gacagttgct cgaactgcgc
    27421 gcacgctcga agacctcggc cgcggtcaag gcactgctgg gtcttgcgcc gaagacggca
    27481 cggcgcttgc gcgacgacgg cagcgaggaa gacatcgaac tcgcccatgt gcacgtcggc
    27541 gatcgcctgc gcgtgcggcc cggcgagaag gtaccggtcg acggcgtcgt gctcgagggt
    27601 cgctcgcaca tcgacgagtc gatgctgacc ggcgagccga taccggtcga aaaagccgcg
    27661 ggcgccgcgg tgatcggcgc caccctcaac ggcagcggca gcctggtgat ccgcgcggag
    27721 aaagtgggct cggccacggt gctcgcgaac atcgtgcagc tggtcgccca ggcgcagcgc
    27781 tcgcgcgcac cgatgcagcg cctcgccgac agggtcgcct actggttcgt gctcgcggtg
    27841 tttgcggtgg ccatcgccac gttcttcata tggggcctgg cgggccccga tccgtcgtgg
    27901 acctatgccg tgctgaacgc cgtgtcggtg ctgatcatcg cctgcccgtg cgcgctcggg
    27961 cttgcgacac caatgtcgat catggtggcc accggccgcg gcgcgcacag cggcgtgctg
    28021 ttccgcgacg ccgaagccat cgagaacctg cgcgcgatcg acacgctgat cgtggacaag
    28081 acgggcacgc tcaccgaagg ccggccggcg tttcgcgccg ccgtgtccgc accaggctgg
    28141 acggaggatg aactgctgcg cctcgcggcg agcctggatc aggcaagcga gcacccgctc
    28201 gccgaggcga tcgttgccga ggcgcggcgc cgcggcctca ccctcgagcc gcccgagggc
    28261 ttcgaatcct cgaccggcgt cggcgtacac ggccgcgtgg gagcgcgacg gctcgtcatc
    28321 ggcaataccg ccctgatgca gcagagcgac atcgacaccg cgagcctggc gaacgatgcc
    28381 gaggctctgc gccaggacgg cgcgagcgtc atgtggatcg gtgtcgacgg cgcgctcgcc
    28441 ggcctcatcg cggtcgccga tccggtcaag gcctcgaccc cggcggcgat cga
//
LOCUS       DAOQVY010000018        28193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_1974_length_28193_cov_7.014251, whole genome shotgun sequence.
ACCESSION   DAOQVY010000018 DAOQVY010000000
VERSION     DAOQVY010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 28193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28193
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1974_length_28193_cov_7.014251"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="PKN91_03120"
     CDS             <1..1178
                     /locus_tag="PKN91_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNS27094.1"
                     /translation="DPALSEIFIVEGDSAGGSAKQGRDRRTQAILPLKGKILNVEKAR
                     FDKMLSSAEVGTLITALGCGIGRDDFDIEKLRYHRIIIMTDADVDGSHIRTLLLTFFY
                     RQIPLLIERGYVYIAQPPLYKIKRGKQEMYIKDDAELNAMLLNSALEDSALHVNAEAP
                     PLRGAGLEMLARKYMEVQAIIQRWSKRYDERLLEQLIYMPEVSADAFDNPEWLRGWTH
                     DLSLRLAALDDGSRSFVVSVREPADGHGTRILVHKHEHGTSTDKFLPREFFESAEYAS
                     IADLGRTLGGLVGEGAFVTRGNDRHDVGSFKEAMRWLFAEARKGQSIQRYKGLGEMNP
                     DQLWETTINPDSRRLLQVRIEDAVAADEIFTTLMGDQVEPRREFIEKNALAVANLDI"
     gene            complement(1198..1803)
                     /locus_tag="PKN91_03125"
     CDS             complement(1198..1803)
                     /locus_tag="PKN91_03125"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HNS27095.1"
                     /translation="MRKFLPAALIAIPALYCFGLLVGAATYPGYSHLTNYASELGAAD
                     APYPWLFNTSIILVGAAAILAGLALPGAFADLRAKRGWTLAAAIMLGLWGVSMVMGGL
                     FPMPDERHGAFGLGLVAPLVPLFMLLALRPVEGSRGMRIFLAIVFAASVVMLAIMFGV
                     GGLVTRANVGLYQRLNSSAGIPWFAVVGIWLLMRARSPRVR"
     gene            complement(1860..2597)
                     /locus_tag="PKN91_03130"
     CDS             complement(1860..2597)
                     /locus_tag="PKN91_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNS27096.1"
                     /translation="MQWIASFVYTTFLFVWVLLFAIFFNIASLVVPLRTRYVLARAWA
                     HVIAGGLKVLCGLDYRIEGRENLPPGGHVALWKHSSSWETFAMVLVCPPQVWVLKREL
                     LWIPFVGWGIRQMHSIAINRGSGHSAVRQVVEQGKRYIEQGIWVMVFPEGTRMAAGET
                     RKYGVSGALLAREAGCLVVPIAHDAGYYWPRRGLLKKGGTITVVIGKPIDPAGRDPRE
                     VNEEAQAWVEETIRGIRARTGGNEKTI"
     gene            complement(2657..3772)
                     /locus_tag="PKN91_03135"
     CDS             complement(2657..3772)
                     /locus_tag="PKN91_03135"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNS27097.1"
                     /translation="MIAGIRCFACEQAHRPQALLTVCSACGMPLRVDYDLAQVRITLA
                     DLARRPANMWRYREVLPVPEGAEVTLAEGYTPLLEVEPNVWVKDEARNPTSSFKARGM
                     AAAVSMAKHLGARALSAPSAGNAAGALAAYGARAGLPVTVAMPADTPRSFFEECRLYG
                     ATVHAVPGTIADAGRFLRENGPRDAFDVSTLREPYRIEGKKTMGYELVEQMQGEVPDV
                     VLYPTGGGTGLIGMWKAFHEMAALGWIAAGRMPRFVSVQAAGCAPVVRGFETGAARTE
                     TWVDPDTRAYGLRVPAPLGGFLCLRAIRESRGTAIAVPEGALLRATAELAARSGIDVC
                     PEGGAAWAALAQLREQGFVRPGARVVVFNTGTGLKYR"
     gene            3783..4514
                     /locus_tag="PKN91_03140"
     CDS             3783..4514
                     /locus_tag="PKN91_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HNS27098.1"
                     /translation="MIELYYWPTPNGHKITLFLEETGVPYRIVPVNIGAGDQFKPDFL
                     AFSPNNRMPAIVDRAPADGGEPIGVFESGAILLYLAEKTGRFIPQDVRGRKSVLEWLF
                     WQMGGLGPMSGQAGHFLNYAPEKIPYAQERYKKEVNRLYGVLERQLARNAFIAGADYS
                     IADMASYPWVLAWERLQQDITDFPAIARWIEAIEARPATLRAYKLGEQYSSRREMTDE
                     EKKILFGQTAASTAQAASKANASPR"
     gene            complement(4521..6596)
                     /gene="glyS"
                     /locus_tag="PKN91_03145"
     CDS             complement(4521..6596)
                     /gene="glyS"
                     /locus_tag="PKN91_03145"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNS27099.1"
                     /translation="MSARDFLVEIGTEELPPKALPELERAFRDGVAGGIDAAGLAHGE
                     VESFAAPRRLAVLVRALASSQPEQEIKRRGPPVSAAVDAAGKPTRAGESFAASCGVAF
                     AALSRVREGKGEFLFYSGRKAGAGTVTLLPAIVQAALDALPIPRRMRWGAGEAQFVRP
                     VHWIVMLFGAETVPATVLDTPSGNATRGHRFHAPAPIPLTAPADYAEALRSRGKVIAG
                     FAERRGKIRAGVEATAAAIGGRAIVGDALLDEVTALVEWPVPLAGRFEDRFLELPREV
                     LISTLQDHQRYFAVEDASGHLSPWFITVSNVESREPAQVRAGNERVVRPRLADAAFFW
                     QQDRREPLAARLPALDAVTFQAKLGSLGAKTQRIVAISGTISANFGWNVGHTKRSAEL
                     AKCDLLTALVGEFPELQGTMGACYAAADGEAEAVVTAIREHYLPRGAGDALPETGPGT
                     AVALADRLDTLAGIFAIGQKPTGTRDPFGLRRAAIGVLRILIEKRIDLDLRSLIDAAV
                     ALQPVANASAADDVYGYVMERLRAYYLEQPGTRYTTEMIDAVLATAPHSPLDFDARLA
                     ALADFLARPEAASLAAANKRIANILKKAEGFTPAAVDASLLQEPAERALATAIATATP
                     GVAAAAAKRDYAAALSAIATLRGVVDDFFDGVMVNAPDETLRRNRLSLIASLQGLVSG
                     IADLSRLPG"
     gene            complement(6593..7528)
                     /gene="glyQ"
                     /locus_tag="PKN91_03150"
     CDS             complement(6593..7528)
                     /gene="glyQ"
                     /locus_tag="PKN91_03150"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNS27100.1"
                     /translation="MSAPVGTPPHTFQDLIFALQKYWAEQGCVILQPYDMEVGAGTFH
                     TATFLRSIGPEPWSAAYVQPSRRPTDGRYGENPFRLQHYYQFQVVIKPSPLPILDLYL
                     DSLRTIGFDPLVHDIRFVEDNWESPTLGAWGLGWEVWLNGMEITQFTYFQQVGGLDCR
                     PVTGEITYGLERLAMYLQGVESVFDLVWTDGPLGRVTYGDVFHQNEVEQSKYNFELAD
                     TAVLFRRFDEHEAACNALLEAKLALPAYEQVLKASHTFNLLDARKAISVTERQRFILR
                     VRTLARGVAQAYYESREALGFPMTKSAPVPKKGAA"
     gene            7575..9611
                     /locus_tag="PKN91_03155"
     CDS             7575..9611
                     /locus_tag="PKN91_03155"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HNS27101.1"
                     /translation="MNAYPHLLAPLDLGYLRLRNRILMGSMHTGLEDKAEDYPRLAAY
                     FAERARGGTGLIVTGGIAPNRAGWVAPFAGKLTRRDEIPRHQLVTRAVHAEGGHICMQ
                     ILHTGRYGYHPLAVAPSRLRSPITPFTPWALSARGVERQIAAFVRCARLAREAGYDGV
                     EIMGSEGYLINQFIAARTNHRSDEWGGEYANRIRFAVEIVRRTRAALGADFILIYRLS
                     MLDLVGDGSNFDEVVHLARAVEAAGATMINTGIGWHEARVPTIATMVPRAAFAWVTER
                     LRPHVALPLVATNRINDPAVAERVLAAGGADMVSMARPLLADPDFARKSAEGRADEIN
                     TCIACNQACLDHVFEGKPATCLVNPRAARETEFDYGPATTRRRIAVVGAGPAGLACAT
                     IAAQRGHEVVLYERAGEIGGQFNMAKRIPGKEEFRETLRYFGRQIELHHVDLRLGTDA
                     TAEQLAQAHDVVVLATGVTPRRPPIAGLEHPKVLSYVDVLLRDAPVGRTVALVGAGGI
                     GFDVAEFLSHADGVSPSLDVDAFLAEWGVDRSPAARGGLAGTHESRAGLAHSAREIYL
                     LQRKVSKPGAGLGKTTGWIHRTTLKRRGVHMLAGVNYERIDDAGLHISNGSESRVLAV
                     DNVVICAGQEPLRDLEAPLRERGVEVHLIGGADVAAELDAKRAIDQGARLAAAL"
     gene            complement(9608..10663)
                     /locus_tag="PKN91_03160"
     CDS             complement(9608..10663)
                     /locus_tag="PKN91_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS27102.1"
                     /translation="MSPVADIPARAASEGPVAYHVSVVALPDAVISTLAGIFDTMSAR
                     SMLGAGQVTRSFDVEIVGESAGPLRLASDLPIEVQRNIDTLESSDIVIVPSVLLRGST
                     WQKGRYPRLVAWLARMHERGAVLCSACSGIFLIAETGVFDGRDATVHFGYADAFARAH
                     PAVTIHPERVLVISGTREELVSSGASSSWHDMALYLIARFAGATDAQAVARMLALQWH
                     QDGLAPYITFEGRTDHGDAEVAAAQQWLGNHFSVANPVEEMIRRSRLAERTMKRRFAA
                     ATGLTPIEYVQRLRIEDAKRRLERTVAPIDEIAWRVGYEDPAFFRRLFKRATGVTPGA
                     YRRRFSIPEFARRGVQV"
     gene            10783..11850
                     /locus_tag="PKN91_03165"
     CDS             10783..11850
                     /locus_tag="PKN91_03165"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS27103.1"
                     /translation="MNAQVIDQSRLEAFATRAIGDIAASYSGIMVTLGSKLGLYTAMA
                     GAGPLSAKEVAGRAGCAERYVREWLNAQAAAGYLRYHASSETYELPPEEAAVLADEDS
                     PTYIPPAWNLPAALWADEPKALEAFRTGRGVAWGEHDARLACGVAAFFRNGYKANLVS
                     NWLPALDGVVPRLEAGIVVADVGCGYGHSTLLMAQAFPNSRFYGFDTHTDSIAEARRL
                     AAAAGVSDRVQFEVARASDYPDHRYDLVCFFDTLHDLGDPVAAARHARKVLAPGGTLM
                     LVEPFAHDRAEDNFSTVGQLYYGGSTLICCAHAIAEGGNLVLGAQAGPRRLQEVFHKA
                     GFTHFRRAAETAFNLILEVRE"
     gene            complement(12035..14296)
                     /locus_tag="PKN91_03170"
     CDS             complement(12035..14296)
                     /locus_tag="PKN91_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNS27104.1"
                     /translation="MQRTAHGIAHITAPDYETLAYGVAYAHAQDNVCQTANQLVTVRG
                     ERSRWFGPQERALLGLREQSNETIDVFIRSHMDDAALAKAFAAASPETQAVSNGYVEG
                     YNRYLRDSAARLPADCAGAPWLRPMTTADYLRLQELTMVQLGVALFADAIFETSPPAD
                     DEPAAKPPGTGQAVVALERFRLNEPLLGSNGWAFGADVTTNGRGVLLGNPHFPWSGVN
                     RFWKMHLTIPGTLDVMGAAIGHTAVVQIGFNHDVAWTHTVSTGQRFTLHELELADGDP
                     HSYVIDGKPVPMTPERVTYEVLDAGGAVQTREHTVWKTRFGPILEVPAAGLAWGRTHA
                     YALKDANTANLRAMDIWLGFNRDRSVGEMRETLAGLGVPWVNTIAADRDGNAMYADVS
                     VVPDVSTAQLERCAPSEAAARLFKAADLTVLDGTRSECDWQHDPRSPVPGLLPIERMP
                     VSVRRDWVQNSNDSFWMTNPAIDWPQITLMVGWTDYPQSLRTRAGLYEIGQRLTGTDD
                     VAPHAKVGIAEVQAMLFRNRNHAAFLALDDFLPVCRETPGEAARAGCAVLATWDRHNN
                     LESRGAHLFREWWRAASAIKGLFSRPFDPADPANTPAGLATDMPEIRAALLKALDDAV
                     ATVRAAGFGLDAPLREVQTYTTPNGVVGVHGGPEPEGVLNKVETASRGPLGADGYPII
                     FGSSYIQTVTFDERGPVAEAMLVYGQSSQVDSPLSFDQLPLYTGKRWMRLPFHAADVE
                     RERVGEVLRLTVE"
     gene            complement(14436..16787)
                     /locus_tag="PKN91_03175"
     CDS             complement(14436..16787)
                     /locus_tag="PKN91_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS27105.1"
                     /translation="MLRRNFLGGKQLIGTTTMAALLALPLCAAAQEGETFEIQEVVVT
                     AQKRTERLVDVPVAISVFSAEAIDQTGVRELKEVTGYMPNVQISSGNDFRSTVTIRGV
                     GATSRNIGFDSRVGVYIDGVYVGQSPAINQELMNLERVEVLRGPQGTLFGKNTVAGAI
                     NLVTKKPDDELRGMASVDIGNYDYREIRAFANLPLGEAVSTSFSVSKTDRDGYVPNIV
                     TGNDLNERDVLAYRAQLRFSPNERFEANASFDGLNSDGRILIGDPVTDMLGMQPVQIA
                     PKMGEVGFNFDPNDERDVYGGSLDLAYELANGHTLKSITGYRTTDAVYSNATDYSPVD
                     IVSIAYRDEYDVLSQELQFISATDKAFSYTAGLYFYHQKANTSRDVILGNDFETYFIG
                     PLYASGALTPPLPAPPALPNNVVRQLLGFGPPLSKVYNSGEVTTESYAAYFNGDWHMT
                     DRWKLGFGVRISTEKKDVDWLLDGRNSGIFNIGSTGPDPLNPTPMINDRTDDFVAPAA
                     SLSYALTPRSNVYARYAAGYKSGGFNLDYINEDELAANQGLEFDKETVDSFELGIKGN
                     YFNGRLSMNLAAFIANYDDYQVNQFVDLGGGRTSIRINNAAKVETTGFEAEATLYATD
                     ELTLHGSLGLLQAEFDSFPGGGSGGTDVSGNELTNAPDLTYSFSAVWKHPVPALRANL
                     LMRGDVTHISEVYTTADNTTTTPYNSAYPGVISFGQLEARTELNARIGLMSDNETWEV
                     YAWGRNLTDETDPTDELRDFFGTIAKLPGMPRTYGVNLVWNFH"
     gene            16863..17615
                     /locus_tag="PKN91_03180"
     CDS             16863..17615
                     /locus_tag="PKN91_03180"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HNS27106.1"
                     /translation="MKKAPHIAPRIVPVIKREGLPEQIVRQLVGLVKSGHLKPGDRLP
                     AERTLAEELGVGRPTLREALRALQLLGIVDIRHGGGVFVTGLDPDTLLGPLHLFLGLD
                     RHKLETILEARKVVEGAVLAFVARVIDDDAIARLQANLAALEAAINSQPHVGTLDVRR
                     VNALAEEFRAIIEGAVSNPILSRAVKGLDSLTTATRNRALSAVPADRLLANHRRIVEA
                     LAQRDPAAAQRALEAHIDYLGEASQVTLRGKT"
     gene            17615..18769
                     /locus_tag="PKN91_03185"
     CDS             17615..18769
                     /locus_tag="PKN91_03185"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNS27107.1"
                     /translation="MRKTTRRAVIAGGITGGVVLAAAGGLWWSRGRKPPLGMEIPEEF
                     RQRGAALLAAHPSVDVHSHPGRSFLVGAKPDSLVIRLMRDGFEADRIADMREARVTAS
                     LFSIVADLPLLALEGGGLSAVREFEEGEAYRDFTRQLARLRSLIADGLIRPGLSPEDI
                     RAAHRAGEHVAVLCSEGGEFIEDRIERLAEAHAAGLRSITLVHYHVNQLGDIQTVAPV
                     HQGLTEFGRAVVGEMNRLGMIIDVAHATQATCAGVVAESRVPVMLSHSSLQSGAEPSP
                     RQISLEHARLIADAGGIIGAWPAGIGSASLADFVAQILALIDAVGIEHVAIGTDMDAN
                     YKPVLTDYADFPLLAAALLVRGLSEADAAKVLGGNFLRLFDDVTAAAGAT"
     gene            complement(19005..19964)
                     /locus_tag="PKN91_03190"
     CDS             complement(19005..19964)
                     /locus_tag="PKN91_03190"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNS27108.1"
                     /translation="MSIDLVLLAACIVGIVQLPLWGKIACTLALAVVFARLFVLGHDA
                     CHGSLFKSEKVNAFVGRLVFLPTLTTFSLWHVGHNVAHHGFANLKGRDQVWVPFSPEE
                     FAALPKWRQRLERVYRSVWGFGLYYLVELWWNKLVFPSRKHVGARRKAFIVDSVLTVL
                     FAVAYLAFLVLVAYATEQPWWLLALFGAVLPFLMWNYIMGTVIFVHHTGPDMVWFDKR
                     SEWMRMRKVDDLTRDIHFPLRLEKLLHGIMLHRAHHYDPFIPNYRLKDATQRLMAHGV
                     ASAKATVAGWKYLRELSRKCALYDYKAKTWLSFPQHEALPATA"
     gene            20501..21910
                     /gene="glnA"
                     /locus_tag="PKN91_03195"
     CDS             20501..21910
                     /gene="glnA"
                     /locus_tag="PKN91_03195"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HNS27109.1"
                     /translation="MTPSDVLKLIKEKEVRYADLRFTDTRGKEQHVTIPAKLVDEDLF
                     RDGKMFDGSSIAGWKGINESDMILMPEPGTAVIDPFIEEPTVNIRCDVIEPATMQGYE
                     RDPRSIGKRAEAYLKSTGIADKALFGPENEFFIFDSVRWNSELRGCSYEIDSAQGAWN
                     SNTIYPEGNKGHRPGLKGGYFPVPPVDAFQDIRNAMCNALEELGQVVEVHHHEVATGG
                     QAEIGVGAGTLVQKADAVQILKYAVLNVAHAYGKTATFMPKPLVGDNGSGMHVHQSLS
                     KDGKALFAGDKYGGLSDLALYYIGGIIKHARAINAFANAGTNSYKRLVPGFEAPVMLA
                     YSARNRSASIRVPWVSNPKARRIEVRFPDSTANPYFAFAAMMMAGLDGIQNKIHPGDP
                     ADKDLYDLEPEEAKGIPTVCHSLDMALEHLDQDREFLTRGGVFTNDVIDAYIALKMQE
                     VTRFRMATHPVELDMYYSC"
     gene            22039..22611
                     /locus_tag="PKN91_03200"
     CDS             22039..22611
                     /locus_tag="PKN91_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNS27110.1"
                     /translation="MPFAILLLASGAAFAGTMYKWVDANGTIHYSDRPQPGATEVHID
                     TRAPASTPPPSVVLATTSAAGAELAEEFRYQSCAIVRPANDETLPNAFSLSVGWQIQP
                     ALRGGDRVTLALDGQLVAGVSPTGTSFTIAPIDRGTHTLMISIVDANGRGMCQSAPVT
                     VHVRQPSLYSPARRPAPTPRPTPRPTPPRN"
     gene            22645..23739
                     /gene="glnL"
                     /locus_tag="PKN91_03205"
     CDS             22645..23739
                     /gene="glnL"
                     /locus_tag="PKN91_03205"
                     /inference="COORDINATES: protein motif:HMM:NF008293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HNS27111.1"
                     /translation="MPVEDAPPSPLSHFDPASLLDALATGIVVLDRQLCVVYANVAAQ
                     DLLGAGFARARGRPFADLLVDSNGLAAILRRSIEHGEAFADRELTFRPSGSSREPRIV
                     DITVTPLADTVTGSHLLLELADATQRQRISREADLLARLDSSRMMIRQLAHEIKNPLG
                     GLRGAAQLLERELADPAQREYTGVIIGEADRLVALVDSMAGPLRPMQRASTNIHELCE
                     HVFTLLRSEAQPTVLLERDYDPSLPDADIDRHQVIQALLNLGRNALQAVGERGRVTLR
                     SRALFGVTIGATRHRLVASLQVEDNGPGVPEELRASLFLPLVTGRANGTGLGLAVAQE
                     IATRHGGMVEFQSAPGHTVFSLLLPLTEVA"
     gene            23736..25145
                     /gene="ntrC"
                     /locus_tag="PKN91_03210"
     CDS             23736..25145
                     /gene="ntrC"
                     /locus_tag="PKN91_03210"
                     /inference="COORDINATES: protein motif:HMM:NF008176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HNS27112.1"
                     /translation="MNERALRVWLIDDDASIRWVLERALRGGGMEPRSFESASSALGA
                     LRSDTPDVLVTDIRMPGTSGLELLKRIRDGWPLLPVIVMTAHADLGNAVSAYESGAFE
                     YLPKPFDVDQAVELVRRAAQTGKRGGIEAGDVPRIPELLGRAPAMQQVFRAIGRLSRS
                     SVSVLITGESGTGKELVARALHEHSPRGKRPFIALNTAAIPSELLESELFGHEKGAFT
                     GADAQRRGRFEQADGGTLFLDEIGDMSTPLQTRLLRVLAEGEFYRVGGQSLVRVDVRV
                     IAATHVDLEARVRNGQFREDLYHRLNVIRIELPPLRARREDVPDLMAHYLRVAAQELG
                     VDAKQLSPGALERLVAWDWPGNVRELVNLCRRLTVLAPGTEIHADDLPAEMSQAQDGG
                     ADVDWSRALEAWAERRTAQDGPWLETAAPEFERTLIRVALRRSQGHRQDAAKLLGLGR
                     NTLTRKLKELGMGADDELL"
     gene            25155..26234
                     /locus_tag="PKN91_03215"
     CDS             25155..26234
                     /locus_tag="PKN91_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNS27113.1"
                     /translation="METLLELLDYRFLGNPVRAWLLAALIFLVTFTVLPAIRGFIERR
                     ARARAAAGEQVEMGRELVSGLVTATLRLFLLVLALYFATRVLTLPAQLDRAVTVLGVL
                     VFWLQVALWAVTATRFALDHQLLRRGPADAARAGSFEVLMFVSRLVIFTFAALLALDN
                     LGVQIKPLLAGLGIGGIAIALAVQTVLGDLFASLSITLDKPFEIGDALLVDDDNGTVE
                     RIGIKSTRLRSLNGEQIVISNADLLQSRVHNFKRMRERRYAFTVAVTYETPRELLAEI
                     PAIVEAAIRAQPQTRFDRCHLLRLGDSGLIFEVVYIVTEPDYRLFADTQQAINFRLLE
                     EFGERGIEFAYPTQRNISEARRPVR"
     gene            complement(26206..27135)
                     /locus_tag="PKN91_03220"
     CDS             complement(26206..27135)
                     /locus_tag="PKN91_03220"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNS27114.1"
                     /translation="MSNSSPLAIEFCGLPFNSPIVLLSGCVGFGEEYTRVEGFSNRDA
                     GAIVLKGTTGKARLGNPPHRVYETPMGMLNAIGLQNPGVDHVVTRILPTLDFSETRFI
                     ANVSGSTLEEYVEVTRRFDDSPIDAVEINISCPNVKEGGVVFGNSPEMSARVVAACRA
                     VTRKPLITKLSPNQTDIRENARRCIEAGTDAFAVINTVMGMAIDTGTRRPVLGNVQGG
                     LSGPAIKPIALLKVREVHEVAAPHKVPIIGQGGIVTARDAIEFLLAGATAIGLGTALF
                     YDPMACKKVNAGITEYLLKHGFASVSELGGAPR"
     gene            27178..27654
                     /locus_tag="PKN91_03225"
     CDS             27178..27654
                     /locus_tag="PKN91_03225"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HNS27115.1"
                     /translation="MFHIVLHEPEIPPNTGNIIRLCANTGAALHLVRPLGFTLDKRSL
                     RRAGLDYDDLATVSVHPDFAACRAALASSRWFAIETGGKRLYSDIAWQPGDALLFGSE
                     RTGLPQAVLATLPPAHHVSLPMRPGNRSLNLSNAVAVVAYEAWRQTGFASGSGTPG"
     gene            complement(27651..>28193)
                     /locus_tag="PKN91_03230"
     CDS             complement(27651..>28193)
                     /locus_tag="PKN91_03230"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNS27116.1"
                     /translation="SPDQAYATSLAQLLSSDTFRAYTSTDIVGVETGGAVKNVLAVGA
                     GLSDGLGFGANTRVALITRGLAEMTRLGVALGARSDTFMGLAGLGDLVLTCTDDQSRN
                     RRFGLLLAGGASVADALREIGQVVEGYPAARAVLKVAQREDVSMPIVEGIHSALYEGV
                     PAIEIVKGLMTRPVRAEVDG"
BASE COUNT         4560 a         9591 c         9594 g         4448 t
ORIGIN      
        1 aggacccggc gctgtccgag atcttcatcg tcgagggcga ctcggccggc ggctccgcga
       61 aacagggtcg cgaccggcgc acgcaggcga tcctgccgct caagggcaag atcctgaacg
      121 tcgagaaggc gcgcttcgac aagatgcttt catccgccga agtcggcacg ctgatcaccg
      181 cactcggctg cggcatcggt cgggatgatt tcgacatcga gaaactgcgc tatcaccgca
      241 tcatcatcat gacggatgcc gacgtcgacg gcagtcacat ccgcacgctg ctgctcacgt
      301 tcttctaccg ccaaatcccg ctgctgatcg agcgcggcta cgtctacatc gcgcagccac
      361 cgctctacaa gatcaagcgg ggcaagcagg agatgtacat caaggacgac gccgagctga
      421 acgccatgct gctcaactcg gcgctcgagg actcggcgct gcacgtcaac gcggaggcac
      481 cgccgctgcg cggcgccggg ctcgagatgc tggcgcgcaa gtacatggag gtgcaggcga
      541 tcatccagcg ctggtcgaaa cgctacgacg agcgcctgct cgagcagctg atctatatgc
      601 ccgaggtttc cgccgacgcc ttcgacaacc ccgagtggct gcgcggctgg acgcacgatc
      661 tctcgctgcg gctcgccgcg ctcgatgacg gctcgcgcag ttttgtcgtg agcgtgcgcg
      721 aaccggcgga cggtcacggc acgcgcatcc tcgtgcacaa gcacgagcac ggcacgagca
      781 ccgacaagtt cctgccgcgc gaattcttcg agtccgccga gtacgcgagc atcgccgacc
      841 tcggccgcac gctgggcggg ctcgtgggcg agggcgcctt tgtcacgcgc gggaacgatc
      901 gccacgatgt cggctcgttc aaggaagcca tgcgctggtt gttcgcggag gcgcgcaagg
      961 gtcagtcgat ccagcgctac aagggcctcg gcgaaatgaa cccggatcag ctgtgggaga
     1021 cgaccatcaa ccccgattcg cgtcgcctgc tccaggtacg gatcgaagac gcggtggcgg
     1081 ccgacgaaat cttcaccacg ctgatgggcg atcaggtcga accgcggcgc gagttcatcg
     1141 agaagaacgc gctcgcggtg gcgaacctcg acatctgagc cgcgcgcgga tcgcgcctca
     1201 gcgcacccgc ggcgatcgcg cgcgcatcag cagccagatg ccgacgacgg caaaccaggg
     1261 gatgcctgcg ctgctgttga gccgctggta gaggccgaca ttggcgcgcg tgaccaggcc
     1321 gccgactccg aacatgatcg ccagcatgac caccgatgca gcgaagacga tcgcgaggaa
     1381 aatgcgcatc ccgcggctgc cctcgaccgg gcgcaatgcg agcagcatga agagcgggac
     1441 cagcggcgca acaaggccga ggccgaaggc gccatggcgc tcgtcgggca tcgggaagag
     1501 gccacccatc accatggaca cgccccacaa cccgagcatg atcgcagccg ccaaggtcca
     1561 gccgcgcttc gccctcaggt ccgcgaaggc gccgggaagc gccagccccg caaggatcgc
     1621 ggccgcgccg accagaatga tgctggtgtt gaacagccac ggatagggcg catccgccgc
     1681 tccgagctcg ctcgcgtagt tcgtcaggtg gctgtagccc ggataggtgg ccgcgccgac
     1741 caacaggccg aagcaataga gtgccggtat cgcaatcagg gcagcaggca ggaacttgcg
     1801 catgaaatcc cccaggtaga gcgtcagcca gccaatcgat ccccttcggc gcgggcgttt
     1861 cagatcgttt tctcgttgcc gccggtgcgc gccctgatcc cgcggatcgt ctcctcgacc
     1921 cacgcctgcg cttcctcgtt cacttcgcgt ggatcgcgcc ctgcgggatc gatcggcttg
     1981 ccgatcacca ccgtgatcgt accgcctttc ttcaacagtc cgcgcctcgg ccagtagtaa
     2041 cccgcgtcgt gcgcgatcgg cacgacgagg cagccggcct cgcgcgcgag cagcgcccca
     2101 ctgacgccgt acttgcgggt ctcgcccgcg gccatgcgcg tgccttccgg gaacaccatg
     2161 acccagatgc cctgttcgat gtagcgcttg ccctgctcga cgacctgcct gacggccgaa
     2221 tggccgctgc cgcgattgat cgcgattgaa tgcatctggc ggatgcccca gccgacgaac
     2281 gggatccaca gcagttcgcg cttcagcacc cacacttgcg gcgggcagac gagcaccatc
     2341 gcgaaggttt cccacgacga cgaatgcttc cacagcgcga catggccgcc tggcggcagg
     2401 ttctcgcgac cctcgatgcg gtagtcgagg ccgcagagga ccttgaggcc gcccgcgatc
     2461 acgtgtgccc aggcgcgcgc cagcacatag cgggtgcgca gcggcacgac caggctcgcg
     2521 atattgaaaa atatcgcgaa cagcagcacc cacacgaaca ggaaagtggt gtagacgaac
     2581 gaggcgatcc actgcatagg cacgccaagt gtaaccgcgc cggcgggcag ccatgggcac
     2641 ggcgaagttc gacggctcag cggtacttga gccccgtgcc ggtgttgaac acgaccacgc
     2701 gcgcgccggg gcgcacgaag ccttgctcgc gcagctgcgc gagcgcggcc caggccgcgc
     2761 cgccttccgg gcacacgtcg atgccgcttc tcgcggcgag ctcggcggtt gcgcgcagca
     2821 gggcgccttc aggcaccgca atcgcggtac cgcgcgattc gcggattgcc cgcaggcaca
     2881 ggaagccgcc gagcggcgcg ggtacgcgca ggccgtaggc acgtgtgtcg ggatccaccc
     2941 acgtctcggt acgcgccgca ccggtctcga agccgcgcac gaccggcgcg caaccggccg
     3001 cctgcaccga cacgaagcgc ggcatgcgtc ccgcggcgat ccagccgagc gccgccatct
     3061 cgtgaaacgc cttccacatg ccgatgagtc cagtgcctcc gccggtcgga tagagcacga
     3121 catccggcac ttctccctgc atctgctcga cgagctcgta gcccatcgtc ttcttgcctt
     3181 cgatgcgata aggctcgcgc agcgtcgaga cgtcgaaggc atctctcggg ccgttctcgc
     3241 gcaggaaccg gccagcgtcg gcgatcgtgc cgggcacggc gtgcacggtg gctccgtaca
     3301 gccggcattc ctcgaagaag ctgcgaggcg tgtccgcggg catggcgacg gtgaccggca
     3361 ggcctgcgcg cgcgccatag gcggcgagtg cgcccgcagc gttgccggcg gacggtgcgc
     3421 tgagcgcccg cgcgccgagg tgtttcgcca tcgacaccgc ggcggccatg ccgcgcgcct
     3481 tgaaggagct ggtcggattg cgcgcctcgt ccttgaccca cacgttcggc tcgacctcga
     3541 gcagcggcgt gtagccctcg gcgagtgtga cctccgcgcc ctcgggcacg ggcagcactt
     3601 cgcgatagcg ccacatgttc gccggccgcc gcgcgagatc ggccagcgtg atgcgcacct
     3661 gcgcgaggtc gtaatcgacg cgcagcggca tgccgcaggc gctgcagact gtcagcagcg
     3721 cctgcggtcg gtgcgcctgt tcgcacgcga agcagcggat gccggctatc atggcgtcca
     3781 ccatgatcga actctattac tggccgaccc cgaacggcca caagatcacg ctcttcctcg
     3841 aagagacggg cgtgccgtac cggatcgtgc cggtgaacat cggcgcgggg gatcagttca
     3901 agcctgactt cctcgcgttc tcgccgaaca atcgcatgcc cgcgatcgtc gatcgcgcac
     3961 ccgccgatgg cggcgagccg atcggcgtgt tcgagtccgg cgccatcctg ctgtacctcg
     4021 cggagaagac gggccggttc atcccgcagg acgtgcgcgg ccgcaagtcc gtgctcgaat
     4081 ggctgttctg gcagatgggc ggcctgggtc cgatgtcggg ccaggcgggc catttcctga
     4141 actatgcgcc ggagaagatt ccctacgcgc aggagcggta caagaaggaa gtgaaccggc
     4201 tctacggcgt actcgagcgc cagctcgcgc gcaacgcgtt catcgcgggt gcggactact
     4261 cgatcgccga catggcgagc tacccttggg tgctcgcctg ggaacgcttg caacaggaca
     4321 tcactgattt cccggcgatc gcgcgctgga tcgaagcgat cgaggcgcgg cccgcgacac
     4381 tgcgcgccta caagctcggt gagcagtact cgagccggcg ggagatgacc gacgaggaga
     4441 agaagatcct cttcggtcag accgcggcca gcacggcaca ggcggcgagc aaagccaacg
     4501 cgtcaccgcg ctgagctgcg tcagcccggc aggcgcgaga ggtcggcgat gccgctgacg
     4561 agaccctgca gcgatgcgat cagcgagagg cggttgcgac gcagcgtctc gtcgggggcg
     4621 ttgaccatga cgccgtcgaa gaaatcgtcc accacgccgc gcagcgtcgc gatggcggac
     4681 agcgcggcgg catagtcgcg tttcgcggcc gcggcggcca ctcccggcgt cgccgtcgcg
     4741 atcgccgtgg ccagcgcgcg ttcggcaggt tcctgcagca gcgaggcatc gacagcggcg
     4801 ggcgtgaagc cttccgcctt cttcaggata ttggcgatgc gcttgttcgc cgcggcgagg
     4861 ctcgccgcct cgggccgcgc gaggaaatcc gcgagcgccg cgagccgcgc atcgaaatcg
     4921 agcggtgaat gcggggcggt ggcgagcacc gcgtcgatca tctcggtcgt gtagcgcgtg
     4981 cccggctgct cgaggtagta cgcgcgcagg cgctccatca cgtagccgta cacatcgtcc
     5041 gcggccgagg cgttcgcaac cggttgcagc gcgaccgcgg cgtcgatcag cgaacgcagg
     5101 tccaggtcga tgcgcttctc gatgaggatg cgcagcacgc caatcgccgc gcggcgcagg
     5161 ccgaagggat cgcgcgtgcc ggtcggcttc tgcccgatcg cgaaaatgcc ggcgagcgtg
     5221 tcgagtcgat ccgcgagcgc gaccgcggtg ccgggtccgg tctccggcag ggcatcgccc
     5281 gcgccacgcg gcaggtagtg ctcacgaatc gcggtcacca ctgcctcggc ctcgccatcg
     5341 gcggcggcgt aacaggcgcc catggtgccc tgcagttccg ggaattcgcc gaccagcgcg
     5401 gtcagcaggt cacacttggc gagttccgca gatcgcttcg tatgcccgac attccagcca
     5461 aaattcgcgc ttattgttcc tgaaatagca acaatacgct gcgttttggc tccgagcgaa
     5521 ccgagcttcg cctggaacgt cacggcatcg agcgccggca ggcgcgcggc cagcggctcg
     5581 cgacggtcct gctgccagaa gaaggcagcg tccgcgagac gcgggcgcac cacccgctca
     5641 ttgcccgcgc gcacctgcgc gggctcgcgg ctttcgacat tgctgaccgt gatgaaccac
     5701 ggcgagaggt gcccgctcgc gtcttcgacc gcgaagtagc gctggtggtc ctggagcgtg
     5761 gagatcaaca cttcgcgcgg cagttcgagg aagcggtcct cgaatcggcc tgccagcggc
     5821 accggccatt cgacgagcgc cgtgacctcg tcgagcagcg catcgcccac gatcgcccgc
     5881 ccgccgatcg ccgcggccgt cgcctcgacg ccggcgcgga tcttcccgcg ccgctcggcg
     5941 aaacctgcga tcaccttgcc acggctgcgc agcgcctcgg cgtagtcggc gggtgccgtg
     6001 agcgggatcg gcgcgggcgc atggaaacga tggccacgcg ttgcgttgcc ggagggtgta
     6061 tcgagcaccg tggccggcac ggtctcggcc ccgaacagca tcacgatcca gtggaccggg
     6121 cgcacgaact gcgcctcgcc cgcaccccag cgcatgcgcc gcgggatcgg cagcgcatcg
     6181 agcgcagcct gcacgatcgc cggcagcagc gtgacggtcc cggcgccggc cttgcggcct
     6241 gaatagaaca ggaactcgcc cttgccttcc ctgactcgcg agagcgcggc gaacgcgacc
     6301 ccgcagctcg ccgcgaacga ctcgccggcg cgcgtcggtt tgccggccgc atcgaccgcc
     6361 gcactcaccg gcggtccgcg acgcttgatc tcctgctccg gctggctgct cgcgagtgcg
     6421 cgcaccaaca cggcgaggcg ccgcggcgcc gcgaacgact cgacttcacc gtgggcgagg
     6481 cctgccgcgt cgatgccgcc cgccacgccg tcgcgaaagg cgcgctcgag ctcgggcagc
     6541 gccttcggcg gcagctcctc ggtgccgatc tcgaccagga aatcgcgcgc gctcacgcgg
     6601 cgcccttctt cggcacgggc gcggacttcg tcatcggaaa gccgagcgcc tcgcggctct
     6661 cgtaataggc ctgcgcgacg ccgcgcgcga gcgtgcgcac gcgcaggatg aagcgctggc
     6721 gctcggtcac cgagatcgcc ttgcgcgcat cgagcaggtt gaaggtgtgc gaggccttca
     6781 gcacctgttc gtaggccggc agcgcgagct tcgcttcgag cagcgcattg caggcggcct
     6841 cgtgctcgtc gaaacggcga aacagcacgg cggtgtcggc gagctcgaag ttgtacttcg
     6901 actgctcgac ctcgttctgg tggaacacgt ccccgtaggt cacgcggccg agcgggccgt
     6961 cggtccagac gaggtcgaag acgctctcga cgccctgcag gtacatcgcg aggcgttcga
     7021 gtccgtaggt gatctcgccg gtgaccggcc ggcaatcgag tccgccgacc tgctggaaat
     7081 aggtgaactg cgtgatctcc atgccgttca gccacacctc ccagccgagg ccccaggcgc
     7141 cgagcgtggg cgattcccag ttgtcctcga cgaagcggat gtcgtgcacc agcgggtcga
     7201 agccgatcgt gcgcagcgag tcgaggtaca ggtcgaggat cggcagcggc gagggcttga
     7261 tcaccacctg gaactggtaa tagtgctgca ggcggaacgg gttctcgccg tagcggccgt
     7321 cggtcggccg gcgcgagggc tgcacgtagg cggcgctcca gggctccggg ccgatcgagc
     7381 gcaggaaggt cgccgtgtgg aaggtgccgg cgccgacttc catgtcatag ggttgcagga
     7441 ttacgcagcc ctgctctgcc cagtatttct gcagggcgaa gatcaggtcc tggaacgtgt
     7501 gcgggggggt gccgacggga gcgctcatgg gtggccgcga agtatagcgg ccggatgagg
     7561 taagttgcgg gggcatgaat gcctatccac acctgcttgc accgctcgac ctcggctacc
     7621 tgcgcctgcg caaccggatc ctcatgggct cgatgcacac gggtctcgag gacaaggccg
     7681 aggactaccc gcgcctcgcc gcctacttcg ccgagcgtgc gcgcggcggg acggggctca
     7741 tcgtcaccgg cggcatcgcg ccgaaccgcg ccggctgggt cgcgccgttc gcgggcaagc
     7801 tcacccgccg cgacgaaatc ccccggcacc agctggtcac ccgggccgtg catgccgagg
     7861 gcggccacat ctgcatgcag atcctgcaca ccggccgcta tggctatcac ccgctcgcgg
     7921 tcgcaccgag ccggctgcgc tcgccgatca cgccgttcac gccctgggcc ctgagcgcgc
     7981 gcggcgtcga acggcagatc gccgcgttcg tgcgctgtgc gcgcctcgcg cgcgaggcgg
     8041 gctatgacgg cgtcgagatc atgggcagcg agggctacct gatcaaccag ttcatcgcgg
     8101 cgcgcaccaa ccatcgcagc gacgaatggg gcggcgagta tgcaaaccgc atccgcttcg
     8161 ccgtcgagat cgtgcgtcgc acgcgcgccg cgctcggggc cgacttcatc ctgatctacc
     8221 gcctgtcgat gctcgatctc gttggcgacg gcagcaactt cgatgaggtc gtgcacctcg
     8281 cgcgcgcagt cgaggcggcc ggcgcgacca tgatcaacac cggcatcggc tggcacgagg
     8341 cgcgcgtgcc gaccatcgcg acgatggtgc cccgcgccgc gttcgcatgg gtgaccgagc
     8401 ggctgcggcc gcatgtcgcg ctgccgctcg tggccaccaa ccgcatcaac gatccggccg
     8461 tcgccgagcg cgtgctcgcc gcgggcggcg ccgatatggt gtcgatggcg cgtccgctgc
     8521 ttgccgaccc cgacttcgcg cgcaagagcg ccgagggccg cgcggacgag atcaacacct
     8581 gcatcgcctg caaccaggcc tgcctcgatc acgtgttcga ggggaaaccc gccacctgcc
     8641 tcgtcaaccc gcgcgcggcg cgcgagaccg agttcgacta cgggccggcg accacgcggc
     8701 gacgcatcgc agtggtgggt gcaggtcctg cgggacttgc ctgcgcgacg atcgccgcgc
     8761 aacgcgggca cgaggtcgta ctgtacgaac gggccggcga gatcggtggc cagttcaaca
     8821 tggccaagcg catcccgggc aaggaagaat tccgcgagac gctgcgctat ttcggccgcc
     8881 agatcgagct tcaccatgtc gatctgcgcc tcggcaccga cgccaccgcc gagcagctcg
     8941 cgcaggccca cgacgtcgtg gtgctcgcga ccggcgtcac gccgcgccgc ccgcccatcg
     9001 cgggcctcga gcatccgaaa gtgctgtcct atgtcgacgt gcttctgcgc gacgcgccgg
     9061 tcgggcgcac ggtcgcgctg gtcggcgccg gcggcatcgg cttcgatgtc gcggaattcc
     9121 tgagccacgc cgacggcgtc tcgccgagcc tcgacgtgga tgcgttcctg gcggaatggg
     9181 gcgtcgatcg atcgcccgcg gcgcgcggcg gcctcgccgg cacgcacgaa tcacgcgccg
     9241 gtctcgcgca cagcgcgcgc gagatctatc tactgcagcg caaagtctcg aagccgggcg
     9301 ctggtctcgg caagaccacc ggctggatcc accgcaccac gctcaagcgc cgcggcgtgc
     9361 acatgctggc gggcgtcaac tacgagcgca tcgacgatgc gggcctgcat atatcgaacg
     9421 gcagcgaatc gcgcgtgctc gcggtggaca acgtggtgat ctgcgccggg caggagccgc
     9481 tgcgcgacct cgaggcccca ctgcgcgagc gcggcgtcga ggtgcacctg atcggcggcg
     9541 ccgacgtggc ggcggagctc gacgcgaagc gcgcgatcga ccagggcgcg cggctcgcgg
     9601 cggcgctcta gacctgcacg ccccggcgcg cgaactccgg gatgctgaaa cgccgccggt
     9661 acgcgcccgg cgtgacgccc gtggcacgct tgaacaacct gcggaagaac gccggatcct
     9721 cgtaaccgac ccgccaggcg atctcgtcga tcggcgccac ggtgcgctcg agccggcgct
     9781 tggcgtcctc gatgcgcagc cgctgcacat actcgatggg cgtcagcccg gtggcggctg
     9841 cgaagcggcg cttcattgtg cgctcggcga gccgcgagcg ccggatcatc tcctccacag
     9901 ggttcgccac ggagaaatgg ttgccgagcc actgctgcgc ggcggcgact tccgcgtcgc
     9961 cgtgatcggt gcgcccctcg aaggtgatgt acggtgcgag gccgtcctgg tgccactgca
    10021 gcgccagcat gcgcgcgact gcctgagcat cagttgcccc ggcgaagcgg gcgatcaggt
    10081 agagtgccat gtcgtgccag ctcgaggaag cgcctgaact gaccagttcc tcgcgtgtgc
    10141 cggagatcac gaggacacgc tccgggtgga tcgtcactgc gggatgagcc ctggcaaagg
    10201 catcggcgta gccgaagtgc acggtggcgt cgcgtccgtc gaacaccccg gtctcggcga
    10261 tcaggaagat gccggaacag gccgaacaca acaccgcacc tcgctcgtgc atcctcgcga
    10321 gccacgcgac gaggcgcgga tagcgcccct tctgccatgt cgagccgcgc agcagcaccg
    10381 acggcacgat gacgatgtcg ctcgactcga gcgtatcgat gttccgctgc acctcgatgg
    10441 gcaggtcact ggcgagccgc agaggtccgg ccgattcgcc cacgatttcc acatcgaacg
    10501 aacgtgtcac ctggccggcg cccagcattg agcgcgcgct catggtgtcg aaaatgccgg
    10561 ccagcgtgga gatcaccgcg tcgggcagcg cgaccaccga gacgtgatac gcgaccgggc
    10621 cttccgaggc ggcccgggcg ggaatgtctg ccaccgggga catgcggctc ccttggcacg
    10681 aatggacctg ttgtgggcga ttctgccact gtcgccgcga ctcgtgtgcc gcgatcctgc
    10741 gccccgcgtg caaccagcac cggatgcgaa gaggaaactt tcatgaatgc ccaagtcatc
    10801 gatcaatcac gtctcgaagc ctttgccacg cgcgccatcg gcgacatcgc cgcgtcgtac
    10861 agcggcatca tggtcacgct cggcagcaag ctgggcctct acacggccat ggccggcgcc
    10921 ggcccgctgt cggcgaaaga ggtcgccggg cgtgccggct gcgccgagcg ctatgtgcgc
    10981 gagtggctca acgcccaggc ggccgcgggg tacctgcgct atcacgcaag cagcgaaacc
    11041 tacgagctgc cgcccgaaga ggcggcggta ctcgccgacg aggacagccc cacgtacatt
    11101 ccacccgcgt ggaatctgcc ggcggctctc tgggccgacg agccaaaggc actggaggct
    11161 ttccgtaccg gccgcggcgt ggcctggggc gaacacgacg cgcgtctcgc ctgcggggtg
    11221 gccgcgttct tccgcaatgg ctacaaggcg aacctcgtgt ccaactggct cccggcgctg
    11281 gatggcgtcg tcccgcggct cgaggccggc atcgtcgtgg ccgacgtggg ctgcggatac
    11341 gggcactcga cgctgctgat ggcgcaggcc ttcccgaact cgcgcttcta cggcttcgac
    11401 acccacaccg actcgatcgc agaggcgcgg cggctggccg cggcagcggg cgtcagtgac
    11461 cgcgtgcagt tcgaggtggc ccgtgccagc gattacccgg accatcgtta tgatctcgtc
    11521 tgcttcttcg acacgctgca cgatctgggt gatccggtgg cggccgcacg gcacgcacgc
    11581 aaggtgctcg ccccgggcgg tacgctgatg ctggtcgaac ccttcgccca cgatcgcgcg
    11641 gaagacaact tctcgactgt gggccagctc tactacggcg gctccacgct gatctgctgc
    11701 gcgcatgcca tcgcggaggg cgggaacctg gtgctcggcg cccaggccgg accgcggcgc
    11761 ctgcaggagg tgttccacaa ggccggcttc actcacttcc gccgcgcggc ggaaacggcc
    11821 ttcaacctga tcctcgaggt gcgcgagtga gcgcctgccc gcgccgccat tcgccgacag
    11881 gattatggta atcaggcgcc aggagccggc gttgtcctac agcgccggct cctggttccg
    11941 ctgtctgtgg ggaccacaca tcccgacccc ggaatggccc gcgcgcaagg cgtacgccat
    12001 gaacagatcc gcaggcacga cctacgtgtg agcgctattc gacggtgagc cgcagcacct
    12061 cgccgacacg ctcgcgctcg acatcggccg catggaacgg cagacgcatc caccgtttgc
    12121 cggtatagag cggcagctgg tcgaacgaga ggggcgagtc gacttgcgat gactgcccgt
    12181 agaccagcat cgcctcggcc accgggccgc gttcgtcgaa ggtcacggtc tggatgtagc
    12241 tgctgccgaa gatgatgggg tagccgtcgg cgccgagcgg gccgcgactc gccgtctcca
    12301 ccttgttcag cacaccctcg ggctcggggc cgccgtgtac gccgacgacg ccattggggg
    12361 tcgtgtaggt ctgcacctcg cgcagtggcg catcgagccc gaagccggcg gcacgcaccg
    12421 tcgcgacggc gtcgtccagc gccttgagca gcgccgcgcg gatttcgggc atgtcggtgg
    12481 cgaggccggc gggcgtattg gccggatcgg ctggatcgaa cggccggctg aagaggccct
    12541 tgatcgcgct cgccgcgcgc caccactcgc ggaagaggtg cgcgccgcgc gattcgagat
    12601 tgttgtgacg atcccaggtc gcgagcacgg cgcagccggc gcgcgccgcc tcgcccggcg
    12661 tctccctgca gaccggcagg aagtcgtcga gcgcgaggaa ggccgcgtga ttgcgattgc
    12721 ggaacagcat cgcctgcact tcggcgatac cgaccttcgc gtgcggcgcg acgtcatcgg
    12781 tgccggtgag ccgctggccg atttcgtaca ggcccgcgcg cgtgcgcagg ctctgcggat
    12841 aatcggtcca gccgaccatt agggtgatct gcggccagtc gatggccgga ttggtcatcc
    12901 agaagctgtc gttgctgttc tgcacccagt cgcggcgcac gctgaccggc atgcgctcga
    12961 tcggcagcag gcccggcacc ggcgagcgcg gatcgtgctg ccagtcgcac tcgctgcgcg
    13021 tgccgtcgag taccgtcagg tccgcggcct tgaacagcct tgcggccgcc tccgagggcg
    13081 cgcagcgctc gagctgtgcc gtgctcacgt ccggcacgac actgacatcg gcgtacatcg
    13141 cgttgccgtc acggtcggcc gcgatcgtgt tcacccaggg tacgccgagt ccggcgagcg
    13201 tctcgcgcat ctcgccgaca ctgcggtcgc gattgaatcc gagccagatg tccatcgcac
    13261 gcaggttcgc ggtgttggca tccttcaatg cgtaggcgtg agtcctcccc caggcaagtc
    13321 cggcggccgg cacctcgagg atgggcccga agcgcgtctt ccagaccgtg tgctcccggg
    13381 tctgcaccgc gccgccggca tcgagcacct cgtaggtcac gcgctccggg gtcatcggca
    13441 ccggcttgcc gtcgatgaca tagctgtgcg gatcgccgtc agcgagctcg agctcgtgca
    13501 gcgtgaagcg ctggcccgtc gagacggtgt gtgtccacgc gacgtcgtgg ttgaagccga
    13561 tctgcaccac ggcggtgtgg ccgatcgccg cacccatgac atcgagcgta ccgggaatgg
    13621 tcaggtgcat tttccagaag cggttgacgc cgctccacgg gaagtgcggg ttgccgagca
    13681 gcacgccgcg accgttggtc gtgacatccg cgccgaaggc ccagccgttc gagccgagca
    13741 gcggctcgtt caggcggaag cgctccagcg cgactacggc ctgcccggtg cccggtggct
    13801 tcgcggccgg ctcatcgtcg gctggcgggc tcgtctcgaa gatcgcatcg gcgaacaacg
    13861 cgacaccgag ctgcaccatc gtcagttcct gcaggcgcag gtaatccgcg gtcgtcatcg
    13921 gccgcagcca gggcgcgccc gcgcagtcag ccggcaggcg cgcggcagaa tctcgcaggt
    13981 aacggttgta gccctcgaca tagccgttgg acacggcctg cgtctcggga ctggcggccg
    14041 caaaggcctt cgccagcgcc gcgtcgtcca tgtggctgcg gatgaacacg tcgatcgtct
    14101 cgttcgactg ttcgcgcagt ccgagcagcg cccgctcctg cggaccgaac cagcgcgagc
    14161 gctcgccgcg aaccgtgacc agctggttcg cggtctggca cacgttgtcc tgcgcatgcg
    14221 cgtaggcgac gccgtaggcg agcgtctcgt aatccggcgc cgtgatgtgc gcgatgccgt
    14281 gggcggtgcg ctggatctcg acgctgcggg cagcaggacc ctgcgcatcg ggtggcggaa
    14341 ggctggcgca gccggcgacc agtgtcgcga gggcggctgc gccggtcgct atccagcggt
    14401 gcggcagagt atcgatggcg gcgctcccga cgggatcagt ggaagttcca gaccaggttc
    14461 accccgtagg tgcgcggcat gcccggcagc ttggcgatcg tgccgaagaa gtcgcgcaac
    14521 tcgtccgtcg gatcggtctc gtcggtgaga ttgcggcccc aggcgtagac ctcccaggtt
    14581 tcgttgtccg acatcaggcc gatgcgcgcg ttgagttccg tgcgtgcttc cagctggccg
    14641 aacgagatca cgccgggata cgcgctgttg tacggcgtcg tggtcgtgtt gtcggcggtc
    14701 gtatagacct cgctgatgtg cgtcacgtcg ccgcgcatga gcaggttcgc acgcagcgcc
    14761 ggcacggggt gcttccacac cgcgctgaac gaataggtca gatccggtgc gttcgtcagc
    14821 tcgttgcccg agacgtccgt tccgccgctg ccgccgcccg ggaagctgtc gaactccgcc
    14881 tgcagcaggc cgagggagcc gtgcagcgtg agctcgtcgg tggcgtagag ggtggcttca
    14941 gcctcgaagc cggtggtctc gaccttcgcc gcgttgttga tgcggatcga ggtgcgcccg
    15001 ccccccaggt ccacgaactg gttgacctgg tagtcatcgt agttggcgat gaacgcggcg
    15061 aggttcatgg agagtcgccc gttgaagtaa ttccccttga tgcccagctc gaagctgtcg
    15121 accgtttcct tgtcgaactc gagcccctgg ttcgccgcga gttcgtcctc gttgatgtaa
    15181 tcgaggttga agccgccgct cttgtagccc gcggcatagc gcgcgtagac gttcgatcgc
    15241 ggggtcagcg cgtaactcag gctcgcggcg ggggcgacga agtcgtcggt gcggtcgttg
    15301 atcatcggcg tgggattcag cggatccggg ccggtcgaac cgatgttgaa gatgccggag
    15361 ttgcgcccgt cgagcagcca gtcgacatcc ttcttctcgg tggagatgcg cacgccgaag
    15421 ccgagcttcc agcgatccgt catgtgccag tcgccgttga aataggccgc atagctctcg
    15481 gtagtgactt caccgctgtt gtacaccttg gacagcggcg ggccgaagcc cagcaactgc
    15541 cgcacgacgt tgttcggcag cgccggcggt gcgggcagcg gcggggtcag tgcgcccgac
    15601 gcatacagcg ggccgatgaa gtaggtctcg aagtcgttgc ccaggatcac gtcgcggctg
    15661 gtgttcgcct tctggtggta gaagtagaga cctgcggtgt agctgaaggc cttgtccgtc
    15721 gccgagatga actgcagctc ctgcgacagc acgtcgtact cgtcccggta cgcaatggag
    15781 acgatgtcga ccggcgagta gtcggtggcg ttgctgtaga cggcatcggt cgtgcgatag
    15841 cccgtgatgc tcttcagggt gtggccgttc gccagctcgt aagccaggtc gaggctgccg
    15901 ccgtatacgt cgcgctcgtc gttcgggtcg aaattgaatc cgacctcacc catcttcggg
    15961 gcgatctgca ccggctgcat ccccagcatg tccgtcaccg gatcgccgat caggatgcgg
    16021 ccatcgctgt tgaggccgtc gaatgacgca ttggcctcga agcgctcgtt cgggctgaag
    16081 cgcaactgcg cgcggtaggc cagcacatcg cgctcgttca ggtcattgcc ggtgacgatg
    16141 ttcggcacgt acccgtcacg gtcggtcttc gacacggaga acgaggtgct gacggcctcg
    16201 ccgagcggca ggttcgcgaa cgcccggatt tcgcggtagt cgtaattgcc gatgtcgacg
    16261 ctcgccatgc cgcgcagctc gtcgtccggc ttcttcgtaa cgaggttgat cgcaccggcc
    16321 accgtgttct tgccgaacag tgtgccctgc ggaccgcgca gcacctcgac gcgttcgaga
    16381 ttcatcagtt cctggttgat cgccggcgac tggccgacgt agacgccgtc gatgtagaca
    16441 ccgacgcggg agtcgaaacc gatgttgcgg ctggtcgcgc cgacgccgcg gatcgtgacg
    16501 gtcgaacgga aatcgttgcc ggaggagatc tgcacgttcg gcatgtagcc ggtgacttcc
    16561 ttcagctccc ggacaccggt ctggtcgatc gcctccgcgc tgaacacgga aatcgccacc
    16621 ggcacgtcga cgagacgctc ggtgcgtttc tgggcggtca cgacgacctc ctggatctcg
    16681 aaggtctcac cctcctgcgc cgcagcgcac aggggcagcg caaggagcgc cgccatggtc
    16741 gtcgtcccga tcaactgctt gccgccgaga aaattcctgc gaagcattgc cgcactcccc
    16801 gtctatcgct agaatggcaa aatggtatta ccagttggcg ggagtgtcaa cggacgacac
    16861 tgatgaagaa ggccccccac atcgcaccga ggatcgttcc cgtcatcaag cgggaaggac
    16921 tgccggaaca gatcgtgcgg caactggtcg ggctcgtgaa gtcgggccac ctgaagcccg
    16981 gcgatcgcct gcctgcggag cgcactctcg ccgaggaact cggagtcggc cgccccactc
    17041 tgcgcgaagc gctgcgcgct ctgcagttgc tcggcatcgt cgacatccgc cacggcggcg
    17101 gcgtgttcgt caccggcctt gatccggata cgctgctcgg cccgctgcac cttttcctcg
    17161 gtctcgaccg ccacaagctc gagacgatcc tcgaggcacg caaagtcgtc gagggggcgg
    17221 tgctggcgtt cgtcgcacgc gtcatcgacg acgacgcgat cgccaggctc caggcgaacc
    17281 tcgccgcact ggaggcggcg atcaattcgc agccacacgt cggcacgctc gacgtcaggc
    17341 gtgtcaatgc gctcgcggag gagtttcgcg caatcatcga gggcgccgtc agcaatccga
    17401 tcctgtcgcg cgccgtcaaa ggtctcgaca gcctgactac ggccacgcgg aaccgcgcgc
    17461 tgtcggccgt tccggccgac cgtctgctcg ccaatcacag gcgcatcgtc gaggcgctcg
    17521 cgcagcgcga cccggcagcg gcgcaacggg cgctcgaggc acacatcgat tacctgggcg
    17581 aggcgagcca ggtgacactg cgaggaaaga cctgatgcgc aagacgacgc gccgtgccgt
    17641 cattgcgggc ggcatcaccg gcggggtcgt gctcgccgca gccgggggcc tctggtggtc
    17701 gcgcggccgc aagccgccgc tcggcatgga gatccccgag gaattccggc agcgcggcgc
    17761 cgcgctgctc gccgcgcatc cgagcgtcga cgtgcacagc catccgggcc gctctttcct
    17821 ggtcggtgcg aagccggaca gcctggtgat ccgcctgatg cgcgacggct tcgaggccga
    17881 ccggattgcc gatatgcgcg aggcgcgggt gaccgcgagc ctgttttcga tcgtggccga
    17941 ccttccactg ctcgccctcg agggcggcgg gctgagcgcg gtgcgcgaat tcgaggaagg
    18001 cgaggcgtac cgcgatttca cccgccagct ggcgcgactg cgcagcctga tcgccgacgg
    18061 cctcatccgc ccgggcctct cgcccgagga catccgcgcg gcgcaccgtg ccggcgagca
    18121 cgtcgcggtg ctctgcagcg agggaggcga attcatcgag gatcggatcg agcggctggc
    18181 ggaagcacac gcggcgggcc tgcgatccat cacattggta cactaccatg tcaatcaact
    18241 cggcgacatc cagactgtcg cgccagtgca tcaaggactg acggaattcg ggcgggcagt
    18301 cgtcggcgaa atgaatcgcc tggggatgat catcgacgtc gcccatgcca cgcaggcgac
    18361 ctgtgccggc gtggtggccg aatcacgcgt gccggtgatg ctctcgcaca gcagcctgca
    18421 gtcgggcgcc gaaccgtcgc cgcgccagat ctcgctcgaa cacgcgcggc tgatcgccga
    18481 cgccggcggc atcatcggcg cctggccggc gggcatcggc agcgcgtcgc ttgcggattt
    18541 cgtcgctcag atcctcgcgc tgatcgacgc ggtgggcatc gagcacgtcg cgatcggcac
    18601 ggacatggac gccaactaca agccggtgct gaccgactac gcggacttcc cgctgctcgc
    18661 cgccgcgctg ctcgtacgcg gcctgagcga ggccgacgcg gccaaggtgc tgggcggcaa
    18721 cttcctgcgc ctcttcgacg acgtcacggc cgccgccggc gcaacctagc ccgcgcgtcg
    18781 gcgcacatcg ccagggccgc gttcgacctc gggtcgctca cgggtcgggc gcgccacctg
    18841 cggtggcgtt ccctcgccgc ggcggcgcga ggcggtgaat ctgactgcgg cagcagaatc
    18901 ctcaccgcta cgcctcgcgc tgctcgccgc ggctcggcgc cctccacgct cgcgacccga
    18961 ggttgaacgc ggccctggcg gcgcgagcgg gatccgcggg gaagctacgc cgtcgccggc
    19021 agtgcctcgt gctgcgggaa ggacagccag gtcttggcct tgtaatcgta gagcgcgcac
    19081 ttgcgcgata gttcgcgcag gtacttccag cccgccacgg tggccttcgc actcgccacg
    19141 ccgtgcgcca tcaggcgctg ggtggcgtcc ttcagccggt agttcgggat gaacgggtcg
    19201 tagtggtgcg cccggtgcag catgatcccg tgcagcagct tctcgaggcg cagcgggaag
    19261 tggatgtcgc gggtcaggtc gtcgaccttg cgcatgcgca tccattcgga gcgcttgtcg
    19321 aaccacacca tgtcggggcc cgtgtggtgc acgaagatca cggtgcccat gatgtagttc
    19381 cacatcagga agggcagcac ggcgccgaac agcgcgagca gccaccaggg ctgttcggtg
    19441 gcataggcca ccaggacgag gaaggcgagg tacgcgaccg cgaacagcac ggtcagcacc
    19501 gagtcgacga tgaatgcctt gcggcgcgcg ccgacatgct tgcgcgacgg gaacacgagc
    19561 ttgttccacc acagctcgac gaggtagtag agcccgaagc cccacaccga gcgatacacg
    19621 cgctccaggc gctggcgcca cttcggcagc gcggcgaact cctcggggct gaacggtacc
    19681 cagacctggt cgcgcccctt gaggttcgcg aaaccatggt gggccacgtt gtggccgacg
    19741 tgccagaggc tgaaggtggt gagggtcggc aggaacacga ggcgcccgac gaaggcgttg
    19801 accttctcgc tcttgaacag gctgccgtgg caggcatcgt gcccgagcac gaacaggcgc
    19861 gcgaacacca cggccagcgc gagcgtgcag gcgatcttgc cccagagcgg cagctgcacg
    19921 atgccgacga tgcacgcggc gagcagcacc aggtcgatcg acacctggaa aatggaacgg
    19981 acggcgctgg gtcggcggtc ggggatggtg atgcctgcgg ccactactcg attactccct
    20041 ggatgggctc acaaccgatt gatgatacag gttctcatct cctgcggaag gggatgcagg
    20101 tctcgacgcg cggtgtaccg acggcctgag gtcaggtacg catggcggac gggcggctcc
    20161 ggtaccgggg ggtcggggct gccccgtacg tggcgacggc ctcctcgacg gacaaccgga
    20221 acgggtcgcg gaccgtgacg ctgcgcctgc gctgccgcgg atcaggctgt cccaccagct
    20281 gaggcgatac cgctcttcgg tctgccgagc gcgagcaagt acctgtgtgt cgatcccacg
    20341 agtccgcaca agtttagtgc agcgccgcgt cgacatgccc tatcatggtg cgtcgtgatc
    20401 acccgcgcgt gcgtaaccca ttgattttct gcatatgaat ggttggcacg ttccctgcac
    20461 tgaaggggtg catccctgaa accctggtgg agagacctgc atgaccccga gcgacgtcct
    20521 gaaactgatc aaggaaaagg aagtgcggta cgccgacctg cgcttcaccg acacgcgcgg
    20581 caaggagcag cacgtgacga ttccggcgaa gctcgtcgac gaggatctgt tccgcgacgg
    20641 caagatgttc gatggctcgt cgatcgccgg atggaagggc atcaacgagt ccgacatgat
    20701 cctgatgccc gagccgggca cggccgtgat cgacccgttc atcgaagagc ccacggtcaa
    20761 cattcgctgc gacgtgatcg agcccgcgac gatgcagggc tacgagcgcg atccgcgctc
    20821 gatcggcaag cgtgccgagg cgtacctgaa gtcgaccggc atcgcggaca aggcgctgtt
    20881 cggtcccgag aacgagttct tcattttcga cagcgtgcgc tggaacagcg aactgcgcgg
    20941 ctgttcgtac gaaatcgatt cggcacaagg tgcgtggaac agcaacacca tctatccgga
    21001 gggcaacaag ggtcaccgac ccggcctcaa gggcggctat ttcccggtgc cgccggtcga
    21061 cgcgttccag gacatcagga atgcgatgtg caatgcgctc gaggaactcg gccaggtggt
    21121 cgaggtgcac caccacgagg tcgcgaccgg cgggcaggcc gagatcggcg tcggcgccgg
    21181 tacgctggtg cagaaggccg acgcagtgca gatcctcaag tacgcggtcc tcaatgtcgc
    21241 gcacgcctac ggcaagaccg cgaccttcat gccgaagccg ctggtcggcg acaacggctc
    21301 cggcatgcac gtgcaccagt cgctttcgaa ggacggcaag gcgctgttcg caggcgacaa
    21361 gtacggcggc ctgtccgacc tcgcgctgta ttacatcggc ggcatcatca agcacgccag
    21421 ggcgatcaat gccttcgcga acgccggcac caacagctac aagcgcctgg tgccgggttt
    21481 cgaggcgccg gtgatgctcg cgtactccgc gcgcaaccgt tccgcgtcga tccgcgtgcc
    21541 gtgggtgtcg aacccgaagg cgcgccgcat cgaagtgcgc ttcccggatt ccacggcgaa
    21601 tccgtacttc gcgttcgcgg ccatgatgat ggcgggcctc gacggcatcc agaacaagat
    21661 ccaccccggc gatccggccg acaaggatct ctacgacctc gagccggagg aggcgaaggg
    21721 catcccgacc gtgtgtcact cgctcgacat ggcgctcgag cacctcgacc aggatcgcga
    21781 gttcctgacg cgcggcggcg tgttcaccaa cgacgtgatc gatgcgtaca tcgcgctgaa
    21841 gatgcaggaa gtgacccgct tccgcatggc gacgcacccg gtcgaactcg acatgtacta
    21901 cagctgctga tgtcggggcg ctgatggtgc ccggcgcatc ggaacgtgag ctgcggtcgg
    21961 cggagtacgt cacaactccg ccggccgctc ttttgtcccc gggtcggccg gcgagtatgc
    22021 tcggtcgata tgcgccgcat gccatttgcc atattgctgc tcgcctccgg cgccgcattc
    22081 gccggcacga tgtacaagtg ggtggacgcg aacggcacca tccattactc ggatcgcccg
    22141 cagcccgggg cgaccgaggt gcacatcgat acgcgagcgc cggcgagcac tccgccaccg
    22201 tccgtagtgc tcgccacgac gagtgcagcg ggcgcggagc tggccgagga attccgctac
    22261 cagagttgcg cgatcgttcg cccggcgaac gacgagaccc tgccgaacgc gttttcgctg
    22321 tcggtcggct ggcagatcca gccggcgctg cgtgggggcg atcgcgtcac gctcgcgctc
    22381 gacggccagc tcgtggcagg agtctcgccg accggcacga gcttcactat cgcgccgatc
    22441 gatcgcggca cgcacacgct gatgatcagc atcgtggacg ccaacggcag gggcatgtgc
    22501 cagtccgccc cggtcaccgt gcacgtgcgc cagccgagcc tgtactcgcc cgcgcgccga
    22561 ccggcaccga cgccgaggcc cacgccgagg cccacgcctc cgcgcaactg atcacgcgcc
    22621 gctgcggcgc ctcccggtgc ggcgatgccc gttgaggacg cgccgccttc gccgctgtcg
    22681 catttcgatc cggccagcct gctcgatgcg ctcgccaccg gcatcgtcgt cctcgaccgc
    22741 cagctgtgcg tcgtctacgc caacgtcgcg gcgcaggacc tgctcggcgc cggcttcgcg
    22801 cgcgcgcgcg gccggccgtt cgcggacctg ctggtggatt cgaacggcct cgcggctatc
    22861 ctgcgtcgct cgatcgagca cggcgaggcg ttcgccgatc gtgagctgac ctttcgcccg
    22921 agcggctcga gccgcgagcc gcgcatcgtc gacatcacgg tcacgccgct cgccgacacg
    22981 gtcaccggat cgcacctgct gctcgagctc gccgatgcga cccagcgcca gcgcatctcg
    23041 cgcgaagccg acctgctcgc gcgtctcgac agcagccgga tgatgattcg gcagctcgcg
    23101 cacgagatca agaatccgct cggcgggctg cgcggcgccg cgcagctgct cgagcgcgaa
    23161 ctcgcggacc cggcgcaacg cgagtacacc ggcgtgatca tcggcgaggc cgacaggctc
    23221 gtcgcgctcg tcgactcgat ggccggtccg ctgcgcccga tgcagcgcgc gagcaccaat
    23281 atccacgagc tgtgcgaaca cgtgttcacg ctgctgcgca gcgaggcgca accgaccgtg
    23341 ctgctcgagc gcgactacga cccgagcctg ccggacgccg acatcgaccg tcaccaggtc
    23401 attcaggcgc tgttgaacct cggtcgcaat gccttgcagg ccgtcggcga acgtggccgc
    23461 gtcacgctgc ggtcgcgcgc gctgttcggg gtcacgatcg gcgcgacgcg ccatcgtctg
    23521 gtcgcgagcc tgcaggtcga ggacaacggt ccgggcgtgc cggaggagct gcgggcgagc
    23581 ctgttcctgc cgctcgtcac cggccgcgcg aacggcaccg gcctcggact tgcggtggca
    23641 caggagatcg cgacgcgcca cggcggcatg gtcgaattcc agagcgcacc gggacatacg
    23701 gtgttcagcc tgctgctgcc gctcacggag gtggcatgaa cgagcgcgcc ctgcgcgtgt
    23761 ggctcatcga cgatgacgcg tcgatccgct gggttctcga gcgcgccctg cgcggcggcg
    23821 gcatggagcc gcggagtttc gaatcggcga gcagtgcgct cggcgcgctg cgcagcgaca
    23881 cgcccgacgt tctggtgacc gacatccgca tgcccgggac gagcggcctc gagttgctga
    23941 agcgcatccg cgacggctgg ccgctgctgc cggtgatcgt gatgacggcg cacgcggatc
    24001 tcggcaatgc cgtatcggcc tacgagagcg gggcgttcga gtacctgccg aagcccttcg
    24061 acgtggacca ggcggtcgaa ctcgtacggc gcgcggcgca gacgggcaag cgcggcggca
    24121 tcgaagccgg cgacgtcccg cgcattcccg aactgctcgg ccgcgcaccg gcgatgcagc
    24181 aggtgttccg cgccatcggc cgactgtcgc gctcgagcgt tagcgtgctg atcaccggcg
    24241 agtctggcac cggcaaggaa ctggtggcgc gcgcgctgca cgagcacagc ccgcgcggca
    24301 agcggccctt catcgcgctc aacaccgcgg cgattccttc ggaactgctc gagtcggaac
    24361 tgttcggcca cgaaaagggc gctttcaccg gcgccgacgc ccagcgccgc ggccgcttcg
    24421 agcaggccga tggcggcacc ctgtttctcg acgagatcgg tgacatgtcg acgccgctgc
    24481 agacgcggct gctgcgcgtg ctggccgagg gcgagttcta ccgcgtcggc ggccagtcgc
    24541 tggtgcgcgt cgacgtgcgc gtgatcgccg cgacccacgt ggacctcgag gcgcgcgtgc
    24601 gcaacggtca gttccgcgaa gacctgtacc accgcctgaa cgtgatccgt atcgaactgc
    24661 cgccgctgcg cgcgcggcgc gaggatgtgc cggacctgat ggcgcactac ctgcgcgtcg
    24721 cggcgcagga actcggcgtc gatgcgaagc agctctcgcc cggggcgctt gaacgcctgg
    24781 tggcgtggga ctggcccggc aatgtgcgcg agctcgtgaa cctgtgccgc cggctgacgg
    24841 tgctggcgcc gggaacggaa atccatgccg acgatctgcc ggcggagatg tcgcaggcgc
    24901 aggacggcgg cgctgacgtc gactggtcgc gggcgctgga ggcatgggcg gagcggcgca
    24961 ctgcgcagga cggtccctgg ctcgagaccg cggcgcccga gttcgagcgc acgctgatcc
    25021 gggtcgcgct gcgcagatcg caggggcacc ggcaggacgc cgcgaagctc ctcggcctcg
    25081 ggcgcaacac gctcacgcgc aagctcaagg agctcggcat gggcgcggat gacgagcttc
    25141 tataattcct gcccatggag accctgctcg aactgctcga ctaccgcttc ctcggcaacc
    25201 cggtgcgcgc gtggctgctg gccgcgctga tctttctcgt cacgttcacg gtgctgccgg
    25261 cgatacgcgg gttcatcgag cggcgcgcgc gggcgcgcgc cgcggcggga gagcaggtgg
    25321 agatgggccg cgaactcgtg agcggactcg tcaccgcgac gctgcggctg ttcctgctcg
    25381 tcctcgcgct ctatttcgcg acccgcgtgc tgaccctgcc ggcacagctc gaccgcgccg
    25441 tcacggtgct gggcgtgctg gtgttctggc tgcaggttgc gctctgggcc gtcaccgcga
    25501 cgcgtttcgc gctcgaccac cagttgctgc gccgcggtcc cgcggacgcg gcgcgcgccg
    25561 gttccttcga agtgctgatg ttcgtctcgc gcctcgtgat cttcacgttc gcggcgctgc
    25621 tcgcgctcga caacctgggc gtgcagatca agccgctgct cgccggtctc ggcatcggcg
    25681 gcatcgcgat cgcgcttgcc gtgcagaccg tgctcggcga cctgttcgcg tcgctgtcga
    25741 tcacgctcga caagccgttc gagatcggcg acgcactgct cgtcgatgac gacaacggta
    25801 cggtcgagcg catcggcatc aagagcacgc gactgcgcag cctgaatggc gagcagatcg
    25861 tcatctcgaa cgccgacctg ctccagtccc gcgtgcacaa cttcaagcgc atgcgcgagc
    25921 gccgttacgc gttcacggtc gccgtcacct acgagacccc gcgcgagctg ctggccgaaa
    25981 tacccgcgat cgtcgaagcg gcgatccgcg cgcagccgca gacccgcttc gatcgctgtc
    26041 acctgctcag gttgggggat tccgggctga tcttcgaggt ggtctacatc gtgaccgagc
    26101 cggattaccg gctgttcgcc gatacgcagc aggcgatcaa tttccggctg ctcgaggaat
    26161 tcggcgagcg cggcatcgag ttcgcgtacc cgacgcagcg gaatatcagc gaggcgcgcc
    26221 gcccagttcg ctgaccgaag cgaagccgtg cttgagcaga tactccgtga tgccggcgtt
    26281 cactttcttg cacgccatcg ggtcgtagaa gagcgccgtg ccgagaccga tcgcggtggc
    26341 gccggcgagc aggaactcga tggcgtcgcg cgccgtgacg atgccgccct ggccaatgat
    26401 cggcaccttg tgcggggcgg cgacctcgtg cacttcgcgc accttgagga gcgcgatcgg
    26461 cttgatcgcc gggcccgaga ggccgccctg cacattgccg agcacgggcc ggcgcgtgcc
    26521 ggtgtcgatc gccatgccca tcacagtgtt gatcacggca aaggcgtcgg tgccggcctc
    26581 gatgcagcga cgcgcgttct cgcggatgtc ggtctggttc ggcgagagct tggtgatcag
    26641 cggcttgcgg gtcacggcgc ggcaggccgc caccacgcgc gcggacattt ccggcgagtt
    26701 gccgaacacg acgccgcctt ccttgacgtt cgggcaggag atgttgattt cgaccgcgtc
    26761 gatcggcgaa tcatcgaagc ggcgcgtgac ttcgacgtac tcctcgagcg tcgagcccga
    26821 gacgttggcg atgaagcgcg tttcgctgaa atcgagcgtc ggcagaatcc gggtgacgac
    26881 atggtcgaca ccgggattct gcaggccgat cgcgttcagc atgcccatcg gggtctcgta
    26941 gacccggtgg ggcggattgc cgagtcgtgc cttgccggtc gtgcctttga gcacgatcgc
    27001 gccggcgtcg cgattggaga agccctcgac gcgggtgtat tcctcgccga agccgacaca
    27061 accggagagc aggacgatgg gggagttgaa tggtaacccg cagaattcga tcgcgagcgg
    27121 actggagttc gacattggtc gattataggg ccacggccta agcttcacgc ccagcccatg
    27181 ttccatatcg tcctgcacga acccgagatc ccgcccaata ccggcaacat catccggctg
    27241 tgcgccaata cgggcgccgc gctgcacctc gtgcggcccc tcggtttcac gctcgacaag
    27301 cgctcgctgc gccgcgcggg tctcgactac gacgacctcg cgaccgtgag cgtgcacccc
    27361 gatttcgccg cctgccgcgc ggccctcgcg agctcgcgct ggttcgcgat cgagaccggc
    27421 ggcaagcgtc tctacagcga catcgcctgg cagccgggcg acgcgctgct gttcgggtcg
    27481 gagcgcacgg gcctgccgca ggcggtgctc gcgacgctcc cgccggcgca tcatgtgtca
    27541 ctgccgatgc ggccgggcaa tcgcagcctg aacctgtcga acgccgtggc cgtggtcgcc
    27601 tacgaggcct ggcggcagac cggcttcgcg tccggttccg gcacgcccgg ctagccgtcg
    27661 acttccgcgc gcaccggtct cgtcatcagg cccttgacga tttcgatcgc gggcacgccc
    27721 tcgtacagcg cgctgtggat gccctcgacg atcggcatcg acacgtcctc gcgctgcgcg
    27781 accttcagca ccgcgcgcgc cgcggggtat ccctcgacga cctggccgat ctcgcgcagg
    27841 gcgtcggcga ccgaggcgcc gcccgcgagc agcagaccga agcggcgatt gcgcgactgg
    27901 tcgtcggtgc aggtcagcac caggtcgcca aggcccgcga ggcccatgaa cgtgtcgctg
    27961 cgcgcgccga gcgccacgcc gaggcgggtc atttccgcaa ggccgcgcgt gatcagcgcg
    28021 acgcgcgtgt tcgcgccgaa gccgaggccg tcggacaggc ccgcgccgac cgcgagcacg
    28081 ttcttgaccg ccccgcccgt ctcgacgccg acgatgtcgg tcgaggtgta ggcgcggaac
    28141 gtgtcggagg agaggagctg cgcaagcgac gtggcatagg cctggtcggg tga
//
LOCUS       DAOQVY010000019        28080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_1988_length_28080_cov_6.063729, whole genome shotgun sequence.
ACCESSION   DAOQVY010000019 DAOQVY010000000
VERSION     DAOQVY010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 28080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28080
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1988_length_28080_cov_6.063729"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..627
                     /locus_tag="PKN91_03235"
     CDS             145..627
                     /locus_tag="PKN91_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNS27117.1"
                     /translation="MDERARVLEYLRAHRVMTIATVGENGPAAAATFYANRGLDLYFL
                     SAPHSRHCRNMEADPRVAVTIHEDYAEWSAIKGIQMTATGRELKGAELAAAQQLYAAK
                     FPDASDSEAASGAIARALSRMSWYELAAVQVRFIDNSRGFGHRTEWSRAQFLATARPE
                     "
     gene            779..1357
                     /locus_tag="PKN91_03240"
     CDS             779..1357
                     /locus_tag="PKN91_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNS27118.1"
                     /translation="MASLASVKSWDDEADVVIAGSGVAGCAAAIEAHDRDPKADILMI
                     EKMPEVLRGGNSRVSGQTLLISKNVEALISYQRNMSRSNPIPEDMLRSWAEQLVALEP
                     YIEERAKEAGNRYVHGGGWCDDQTVNEFPELGAEAAVERVATILPVPSGTWLTFDKCV
                     ALRPRVRRIFECALVDLVQDPDTLEVLGVIVE"
     gene            1376..2290
                     /locus_tag="PKN91_03245"
     CDS             1376..2290
                     /locus_tag="PKN91_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNS27119.1"
                     /translation="MRARRGVVMAIGGFENNMQMLRDYNGMTDVLPFGTPGNTGDGIK
                     ILQKAGADMWHLRNRVQSSGIWPGFRYPGRNTVFKRHLFFQAFSWIDIAQDNTRFVNE
                     TAEYRLNHYKVREHGEWIDPPHQRVGRMHMIFDEATRQGTCLVIGIMTWNSIVEGYEW
                     SEDNSKEVAAGWIVKADSIEELARKIDRDPASVAATVREYNEACAAGHDAKFGREPQT
                     LQPIAKPPYYAIEVVPSIVCTGGGARRNIESEVLDHGGKRIPRLYEAGELGSMFSDLY
                     QNGSYLTEAMISGRAAGRNAVQQKNWAT"
     gene            2476..2673
                     /locus_tag="PKN91_03250"
     CDS             2476..2673
                     /locus_tag="PKN91_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27120.1"
                     /translation="MLVKTPAMRIDVHVESVKVDGKHLLLAGLAGHLPCEMRAGAPEL
                     RALLRLALKPRILLWLLRGKT"
     gene            complement(2743..4134)
                     /locus_tag="PKN91_03255"
     CDS             complement(2743..4134)
                     /locus_tag="PKN91_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNS27121.1"
                     /translation="MIIDDDARYGAWLTAHVGAVLPDAQVEFVDIPEFEQRRATVRFQ
                     DWDVLLLAMNFGNSPEDAAAEGLGWLRRLRDQTLLPPIIAIAAGGNELTAVRAIRLGA
                     SDYLPKHLLTPSRLNTAIKIALRAVERRRAADRSRKSEPAPAPRSVPPGGRARLPRSL
                     IPRYAILDVLGESDRAVVYLATSFELDSLVALKVTRTGDDSLPAVDGAPRERTVRQDF
                     AREYEVISSIHDPSVIDIYDYGVHDGLEYLAMEYFPRGDLKARLQHPMDAGDALDYVR
                     RIARALGVVHAAGLVHRDLKPPNIMLRENDEVVLIDFGLARQMHAEITTTSTGMLRGS
                     PYYMSPEQAQGLPLDARSDLYSLGVVLFEMLTGRKPFTGSSAIEVLQRHVTAPVPLLP
                     AEHAHLQPLIDRLLAKTPARRYASAEELQVALAQAAGPDAGRASAGAPARVHDVAVEV
                     SDDDHETPSAALA"
     gene            complement(4161..6140)
                     /locus_tag="PKN91_03260"
     CDS             complement(4161..6140)
                     /locus_tag="PKN91_03260"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNS27122.1"
                     /translation="MQSGFAPVGAPAGLVALTRDPALVAALRVLVEDGHRFEFVASDT
                     ALSDHLMASQTRAALIDSAAASVPVAELTARLKAQFPDLVLVVAGTGAEQGVLAPQIT
                     DGRVFRFLHKPVSAQRVKLFVESAFRHSGALPAAPTASPPTHSSGARSRTPLVLGALA
                     LAIVAGAAIWWTTREAPPAAVAVDAPAAAPRSSAPANAQPAQAAPSDLDPATQRLLER
                     ADAALASGQLVSPPGESAADLYKLVLAKSPGHTRATAGLDDIANRHLAQAEQAIVAEE
                     LDEATRLVDVARAVLPDHPRVAFLATQIGKERERQLLTVARQAAASGNLDRAIAVLES
                     GSSGGSELIGAARRELQQQEIDLQAANFLALADARVREGALLAPAQDNARYYIESARA
                     LSPEHAGLREALRKLQAALVDDARAAISAGDIAAAELRITAALDNGVAHGELDALRSE
                     LAAARVALEADAFTALGQRFNAQLRANRLLEPAVDSALALYAQMREANAQHPQTLNAR
                     NALGGRMLAESGAALARGDIAGAERWVGEAEAIGFTGLELTNAKRDIATQRTRAQRLN
                     EAVPVSQLTRTRMLEPRYPAAALAAGQEGWVDLEFTVTPTGTVEDVQVTGSSPAGVFD
                     EAASDALSRWRFRPVEANGVAVPQRAKLRMRFDLQ"
     gene            6351..7286
                     /locus_tag="PKN91_03265"
     CDS             6351..7286
                     /locus_tag="PKN91_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27123.1"
                     /translation="MVRNVRIVTLLVLALIAPAAWSAGVSGAQERAAQEFLAAVASGN
                     TQAIAASLHPDELRRVRAGITARLRADAAVGDHRVRSRLFGEASNLGDIERLTDQSFF
                     AAFGKRLRYPGRLFAKVKGLEAVKDGDDIVHIVIRGEQPEGRGKMRVVTLVTLMAEGR
                     GWKAAMPSEVEAQIEDLLDGREPATGLVPLAPVASAAPGAAQAVNSPEIRSLLSSAAG
                     ALLGGHCDEYYGEYMSPNFRRSTSARALGTLVKSCERSDSMREMLVATLRIVDGSSPR
                     YEQDGARARYDITGRGLPYDHFVLEKIDGRWYIAE"
     gene            7283..7759
                     /locus_tag="PKN91_03270"
     CDS             7283..7759
                     /locus_tag="PKN91_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS27124.1"
                     /translation="MSEPRIRPALPADAPVLLSLIRGLAEYEKLAHEVVATEAQLAAE
                     LGAPDPVVHALIAERAGEPVGFALYFFSFSTFLGRRGLYLEDLFVLPAQRGHGIGRAL
                     LAELAGRAAERGCGRMEWSVLDWNVDAQRFYAGLGARPLSGWTVYRLDRAAIAALG"
     gene            7791..7991
                     /locus_tag="PKN91_03275"
     CDS             7791..7991
                     /locus_tag="PKN91_03275"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HNS27125.1"
                     /translation="MSVAKVSEIIASSTKSFDDAIATGVKRATKTLTGVQSAWVADQE
                     VHVKNSKIIEYRVRLKVTFVLK"
     gene            complement(7972..9810)
                     /locus_tag="PKN91_03280"
     CDS             complement(7972..9810)
                     /locus_tag="PKN91_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNS27126.1"
                     /translation="MSSTDPRTQVPGRDPLDGESLAAALRAGIYRLFARTDHLNSINV
                     FPVPDGDTGTNMAMTMSAVLARLDTQRTAHAGELLARVADAAIDGARGNSGAILAQFL
                     HGVADRVGPLATIGGTEFAAATDTGARYARDAVTEPQEGTLLSVLAALAAELRRVAAG
                     GPIGVGQSLRETLPALRAALAATTGSLSVLRAANVVDAGAAGFIEIVEGMSSYLETGE
                     IGAAVTPPHDAVEAMSAGGAEGAHRYCTECMVSGEGVDQRRLREELTTLGSSLVIAGT
                     RSKARIHIHTNEPARVFDVAAHFGALSGQKADDMHRQAHALHRTGAARVAIVSDSGAD
                     IPEELLEKLDVHIVPLRVHFGARSYLDKVSLSPQEFYRELATGGVHPQTSQPPPGDFR
                     RMYEFLASHYAHVVSITVSGRVSGTHAAASSAATRVAGGKVTVIDSLNASLGQGLLAI
                     HAAECALAGYDGAQLVAAVESMRAKTRTWALLVRLDYAVRGGRVTPLVQKLSRALRCS
                     PVLTNLPDGRVKPRGALFGRGDPVPRFARFVARRLRRGVAYRITVGHADAQREGERLL
                     DALRSCVPQVESAHLTPLGTALGVHGGPGMLVVGVQEYAAPTSGRR"
     gene            complement(9797..10546)
                     /locus_tag="PKN91_03285"
     CDS             complement(9797..10546)
                     /locus_tag="PKN91_03285"
                     /inference="COORDINATES: protein motif:HMM:NF027851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HNS27127.1"
                     /translation="MSFILDALKKSDSERQRQSGPALFEIRKAPPRGGLPFWAIALGA
                     LLLVNLVVLAWFMMREPQTAPAQQGAPATAPRADAPAADTPAAATLTPTPTGTPVPAG
                     SPPGVAPQAGTAQVLPPQVAAPAPDLSAGAALAQDDAAYAPPDYSGNPADYEQALPVG
                     PAPDAATQAAERGMVPTLEQLPPAIANQLPPLRLDLHVYATRAADRFVMINMRRLREG
                     DATPEGIRLEEITPSGAVMSFRGTRFQLEQH"
     gene            complement(10550..12214)
                     /locus_tag="PKN91_03290"
     CDS             complement(10550..12214)
                     /locus_tag="PKN91_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS27128.1"
                     /translation="MYLSFFGLHEKPFSITPDPRYLYLSERHAEALAHLLYGVSEAGG
                     FVQLTGEVGTGKTTIVRSLLGQIPKNAEVALILNPRMTAPEFMLTICEELGIGLPDRA
                     VASHKDLVDILNHYLLRAHAEGRRVVLLVDEAQNLDATVLEQVRLLTNLETATQKLLQ
                     IILIGQPELREMLARAELRQLAQRITGRYHLDPLSRAEASAYVLHRLRVAGATTEIFT
                     SGALAEVYRQSGGIPRLINVICDRALLGAYTQDRHRVTATIVRNSASEVFGRRIAPGW
                     LPWAAGAGGALVTILAAVALWQSGVLDRGARDAAGALSGASGSVAAIPEASVAAEPPA
                     PPAPSVPSLPTLGPLLAGAGSATGSDAAYSRLLARWNVTYTPGAGDACAQAELAGLQC
                     VAQRGSFAQLRVHDRPALLLLGDEQGGAHQVVLTALDGDTARLDIGGAEHTVGIADLA
                     RYWFGDFVVLWRPIALPVRALAPGMSGDSVLQLRRSLEQVAGLSPADASSDRYDASLV
                     RLVEDFQRSRRLHVDGIAGLQTQLALDAAMPAAGTPRLITASAGEG"
     gene            complement(12276..13934)
                     /locus_tag="PKN91_03295"
     CDS             complement(12276..13934)
                     /locus_tag="PKN91_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNS27129.1"
                     /translation="MARPWLESYPADVRWDIDDQPAPAWRMLDTAVASWPDHPALTFF
                     ARKLSYRELHALVDRAAAGLQRLGVRPGDRVGLYLPNCPQYPIAFFGALKAGAIVVNY
                     SPLDAEATLARKVEDSGTDVLVTLDLEVLLPKAESLLAGTRLKTVIVGSLADFAGVPI
                     PPQCEVPRDAAHLRFLELLDNDGRYTPQPIDDPATHIAVLQYTGGTTGVPKGASLTHG
                     NISMAAAQCVETTRAEPRSMLPGEERMLLVLPLFHIYAELIMFLGISLGTELVLHPKF
                     DVAAVANDIAARRITVMFGVPTMFLALAAHARAHPIDLSSLKHCGSGGAPLALEIVEQ
                     FRQLTGVQVTDGWGMSETTAAGTFTPRHGLQKPGSCGLPLPRVDMKIVATDDAGRELA
                     PGELGEICIRGPNVMRGYWQQPQDTAAAFTPDGWFRTGDVGYIDGDGCVFIIERCKDM
                     LLCSGYNVYPRVIEDAIYAHPDVAEVCVIGVADEYRGQSPKAFIRLKDGAPELTLEAL
                     QEFLSGRVGRHEMVRALELRAELPKTPVGKHSKLALYEEERRRR"
     gene            complement(13938..15116)
                     /locus_tag="PKN91_03300"
     CDS             complement(13938..15116)
                     /locus_tag="PKN91_03300"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNS27130.1"
                     /translation="MTDAVIVSIARTPLAKSWRGSFNITHGATLGGHVVQHAIARAGI
                     DPAEVEDVLMGCAYPEGATGMNVARQSALRAGCPVTTGGVTINRFCSSGLQSIAMAAA
                     RVIVEKTPIVVAGGVEAISCVQNEMNTHMLREPWLAEHKPDLYLPMLQTAENVARRYS
                     ISRERQDEYGARSQQRAAAAQAAGLFDAEIAPITVTAGYADPKTQQLGTREVTVAKDE
                     GIRPDTTFEGVSKIRPAIPGGVIAAGNASQFSDGAAACVVMDATLAEKRGIQPLGIFR
                     GFTMAGCEPDEMGIGPVFAVPRLLERAGLGIDDIGLWELNEAFAVQVIYCRDRLGIPD
                     ERLNVNGGAIAVGHPYGVSGTRLAGHALIEGKRRGVKYVVVTMCIGGGMGAAGLFEVV
                     "
     gene            complement(15133..17223)
                     /locus_tag="PKN91_03305"
     CDS             complement(15133..17223)
                     /locus_tag="PKN91_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNS27131.1"
                     /translation="MGVTTYEKHGKVAVITMDSPPVNGLGQPLRAALLKSLDKALAAR
                     GVKAIVLIGSGKAFSAGADIREFNTPKSYAPPNLLTLLSAFEASTKPVVAAIHGSCMG
                     GGLELALACHYRVALPDAQIALPEVKLGLLPGAGGTQRLPRALGLEAALNMIVSGSVV
                     PAKAFAGSALLDDILEGDLRAGAIDYATRLIKEKRPAKRVRDLAVKHPNLEGFLMFAR
                     NTVESVAGPWPAPRKCVEAVLAAATTPFETGMATERRLFSELMQSPESKAARHIFFGE
                     RAAAKIPDVPDSTPTRPIARAAVIGAGTMGGGITMNFINAGIPVTLLEMKQEALDRGL
                     ETIRRNYGGAVKKGRMKEADVESRMQLITPALDYAAIAQADIVIEAVFEEMSVKQAVF
                     EKLDAVMKPGAILASNTSTLDLNRIAGFTNRPGDVIGTHFFSPANVMRLLEVVRGAKT
                     APDVLATVMQLAKKMRKIGVVSGVCDGFIGNRMLEQMGRQTLFLLEEGALPKQVDAAM
                     EKFGFAMGPFRVADLAGNDIGWAIRKRRYVEKPLVTYSRVADRLCEQGRFGQKTGAGW
                     YDYKEGDRTARPSAAVEQMIVDYSKEIGVKRRAISDEEIVDRVVYALVNEGAKILEEG
                     IALRASDIDMVYLNGYGFPPHRGGPMLYADTVGLDVVVRRMKQFARNPHGDPLAWEPA
                     RSLAKLAAAGKTFS"
     gene            17410..18555
                     /locus_tag="PKN91_03310"
     CDS             17410..18555
                     /locus_tag="PKN91_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNS27132.1"
                     /translation="MELAFSAEDEAFRSEVREFVRAKLPLGLRNKVVAGERLAKSEYL
                     TWHRILFERGWIAPNWPAAFGGCEWSAIRQHIFDEECALAGAPGILPFGIRMVAPVIM
                     KFGNAAQQQRFLPRILSGEDWWCQGYSEPGAGSDLASLKMRAERRGGHYLVNGQKTWT
                     TLAHYADWIFCLVRTSTEGRQQEGISFLLVDMQSRGVSVRPIIMLDGGHEINEVWFED
                     VEVPAENLVGEEDKGWTYAKYLLGHERTGNAGVGHCRRWLGELKSLARRERSNDGRPL
                     VDDPRFRDRIARLEIDLMALEVINVRVLMLEQQRRAPGPEASMLKIMGSDLQQALTEE
                     TMRASGHGAMRYPQPGTGHYFNFRKASIYAGSNEIQRNIIARHILGF"
     gene            18558..19691
                     /locus_tag="PKN91_03315"
     CDS             18558..19691
                     /locus_tag="PKN91_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNS27133.1"
                     /translation="MITLTDEQQQFRDSLQRMLRTDYSFERRREIAASDSGWSEATWS
                     GLAELGALALPLPEAHGGLGGGAMDVLVVMEECGRALLLEPYLATVVMGAGLVARIGS
                     DAQRAAILPAVAAGECRLALAHYESAGRYALDAVATEARPGGAGWILAGEKHSVLHGA
                     AADRLLVSARTPRGLSLFLVERGAPGLAVSDYPQYDGQRAATIRLDQVRVDAGALLGG
                     EGAAAEAIEYTIDAAIAAVCAEAVGAMGALLEATGQYLKTRRQFGVPIGSFQVLQHRM
                     ADMLLETEKARSMALLAASRLADATRDERRRIVSAAKVKVGEAARLVGQQAVQLHGGI
                     GVTDELAVGHYFKRLTMIELAFGDIDYHLGQVGSRIFDGTSTG"
     gene            19712..22171
                     /locus_tag="PKN91_03320"
     CDS             19712..22171
                     /locus_tag="PKN91_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS27134.1"
                     /translation="MRSLRGVMRAILGCAAVSLACGGTAVAQDQDSERGRTLDEVLVT
                     AQKREQNVQDVPIVVTAVSGQLLQDSGVHDIKDLTLLVPGLIVTSTSNETSTTARIRG
                     MGTVGDNIGLESSVGVVIDEVYRPRNGVGFGDLGELERIEVLKGPQGTLFGKNTSAGV
                     INVKTKAPSFDVDADVALSAGNYGLLEQSFALTGPIAGDKLAGRIFLAHREYDGLLDV
                     GTGIGGRGDTTDMDREVNTVRGQLLWRPGDNFEARLIADWSERNESCCLGVQYVTGPT
                     GPIVAALSGGAIAVPADPFERRAYANRDTIQDIRDHGASLQMDWDLAGGATVTSVTAW
                     RNWETENGQDSDFSGAGILFRDSKNYSNTFETLTQELRIAGQNGRLTWLAGAFYADED
                     LESINALNYDSQFEPYVSLLLTQGASTALLSPLLGRAPGTSYTLGFGSYDTYAQSSKS
                     WALFTNNSLAITDQLELTLGLRYTDEEKKLTSNYDNIGDGVGCAAVRARAPILPLILA
                     GAGIPAASHAAIIAGLGSLGCANFSDPLYNDFSHSQKISEGEWSGTAKLAYRFTDDAM
                     GYASYARGYKASGFNLDRERTSVNPYEPTVAVDTDTTFAPEEVDSWELGVKTTWAGNS
                     LALNAAAFYQEYTGFQLNTFTGTVFVVASIPEVTSEGVDFDFTWYTPLESLSFQGGIT
                     YADTKYGDFVPTFANGQRLPGARMSFAPEWSGTLAATFEHHFGANLKWRLNVGAKYMS
                     EFNTGSDLNPTKEQDAFTLVDARVGLGSRNDSWALELWGRNVFDEEYIQLGFDATLQP
                     GTTNAFLGNPQMFGATFRLKF"
     gene            complement(22205..22579)
                     /locus_tag="PKN91_03325"
     CDS             complement(22205..22579)
                     /locus_tag="PKN91_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27135.1"
                     /translation="MAHVELPRALEHYFAHAETAPTRSGRRFSITMPYLDGERLDRLQ
                     WWWQLPATQEKLGGEDIVAKLRAEAVERFSRHIERWLANTGQRFHGSGPIPCIGRAEN
                     APAAEKQAPAEALPAMDSVVNG"
     gene            complement(22587..23795)
                     /locus_tag="PKN91_03330"
     CDS             complement(22587..23795)
                     /locus_tag="PKN91_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNS27136.1"
                     /translation="MDFEYNARTRELLARLQAFMDEHVYPNEAEFAAQVAEGDRWQPT
                     RLIEELKAKARAEGLWNLFLPDSRHGPGLTNLEYAPLCEVMGRVIWSPEVFNCSAPDT
                     GNMEVLERYGTAEQQRRWLAPLLAGEIRSCFAMTEPTVASSDATNIETSIVRDGNDYV
                     VNGRKWWSSGASDPRCKLLIVMGKTDPQNPSRHRQQSMILVPRETPGITILRSLPVFG
                     YDDAPHGHGEILFEDVRVPATNLLQNEGDGFSIAQGRLGPGRIHHCMRLIGLAERALE
                     KMCRRALARVAFGKPVAAQTVTLERIADARILIDQARLLVLKAAHMMDTVGNKAAAKE
                     IAMIKVAAPVMACQVIDWAIQVHGAAGVSDDFGLAHAYAQARALRFADGPDEVHRNHI
                     GKLELAQYRI"
     gene            complement(23798..24778)
                     /locus_tag="PKN91_03335"
     CDS             complement(23798..24778)
                     /locus_tag="PKN91_03335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HNS27137.1"
                     /translation="MKALLCRQFGPPETLVVEELPDLVPGDGQVVIDVHAAGVNFPDS
                     LIIEGRYQFKPPLPFAPGGECAGVVRAVGTGVKNARVGDRVIAFTVYGTFATQVAVDA
                     ARLVPMPPGLDFPTGAAFVMTYATSYHGLVDRGALQAGETLLVLGASGGVGLAAVEIG
                     KALGARVIAAASSAAKLAICREHGADELIDYSNENLRERLKALTGGRGVDVVYDPVGG
                     IYTEPALRSCAWRGRHLIVGFTNGEIPRIPANLALLKGCAIVGVFLGDYLHREPARAA
                     ADLRTLFEWLGAGKLRPHIAGTYPLARGGEAIRALVERRVSGKLVILPGE"
     gene            complement(24775..25626)
                     /locus_tag="PKN91_03340"
     CDS             complement(24775..25626)
                     /locus_tag="PKN91_03340"
                     /inference="COORDINATES: protein motif:HMM:NF004843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNS27138.1"
                     /translation="MRELTGRVAVVTGAASGIGRELALACAREGMRLVLADLDRAGLD
                     ATLALLPRAGGPTSPAAAPLAVTCDVSKPDSVERLAAASWDTFGDVHLLFNNAGVAVS
                     GPVWAATIEDWQWSLGVNLMGVVHGIRSFVPRMLERGQEGHIVNTASVAGFISVPGSA
                     VYCATKHAVVTLSECLLHDLALVKSRLAVSVLAPAFVNTGIADSARNRPAELAATNPL
                     AAPFEERMRRAICAGRLSAADIAQLTIEGVKADRFYIISHPAILPSIEARAREILEGR
                     IPVSPMP"
     gene            complement(25632..26402)
                     /locus_tag="PKN91_03345"
     CDS             complement(25632..26402)
                     /locus_tag="PKN91_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNS27139.1"
                     /translation="MASTPSFDLSGRVALVTGASRGIGESIARALAGAGAHVIVSSRK
                     LEACEAVAASIRAAGGEASALACHIGELDEIAKAFAAIESAHGRLDILVNNAATNPHY
                     GPITSIDPAAFQKTVDVNIRGYFFMSQAGAKLMARGGGGSIVNVASINGVIPGPLQGV
                     YSITKAAVISMTKAFARECAPEGIRVNALLPGLTDTKFAATLTRNPAVLEKVLPHIPM
                     NRAAEPDEMAGAVLYLVSPAASYTTGVALNVDGGYLVA"
     gene            26474..27385
                     /locus_tag="PKN91_03350"
     CDS             26474..27385
                     /locus_tag="PKN91_03350"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNS27140.1"
                     /translation="MHIRGIDLNLLVVLDAIITEGGITRAADKLNLTQPAISHALGRL
                     RILFDDPLFVREGRAMRATPKARSLAEPVRRALRGLEVMLTETESFDPATSQRRFTIG
                     VRDVFEATLLPPLMRAIQRLAPHVQVAALMVPRGELESELAAGRLDAALDVRWPMSEA
                     IRRQRIAEERLVVVVRQRHPIVRRGLDLDQYLAQEHIVVSSRRSGEGPLDQELARLGL
                     RRRMRLRCQHYFAACRVAAMTDLLLTMPEQYARIVNKKLPNQVLPLPFDMAPLDAYLY
                     WHAEADRDPANRWLREQLARVFAPRAR"
     gene            complement(27382..>28080)
                     /locus_tag="PKN91_03355"
     CDS             complement(27382..>28080)
                     /locus_tag="PKN91_03355"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27141.1"
                     /translation="VITVAELPGRLDALPIRRLPGLGRKKGDEVERAGIGTLGELRRA
                     PDAVLWPLFGRDTRRMRERAGGIDDRPVVPDREDLSISAEETFDTDLADPARLEAELL
                     ALADRASARLRRKELAAGCVAVKVRRADFATFTRQQQLEPPTQDSRVIGRAACDLLRK
                     WRAEHPRARVRLLGVGVTGLAPATQLDLFAAGSVPQPPPLDSALDAIRERFGSSVLTR
                     ASGLPPGKRKDAPR"
BASE COUNT         4378 a         9512 c         9858 g         4332 t
ORIGIN      
        1 acagcgtcgg gcgcgtgctc gacctcttcg atattgacgt gccggggatg aggcgctggg
       61 agggcgtcaa tacaaatgat cgccctccgg acccggcagc ttcgtaggag cgggtgagag
      121 tcctctcttc cgggagcaat cgacatggac gagcgtgcgc gagtgctcga gtatctccgg
      181 gcccaccgtg tgatgacgat cgcaaccgtc ggtgaaaatg gcccggccgc tgccgcgacg
      241 ttctacgcga atcgggggct cgacctctac ttcctctccg ctcctcatag ccgccactgc
      301 cgcaacatgg aggctgatcc ccgggtggcg gtcacgattc acgaggacta cgcggagtgg
      361 tccgccatca agggtatcca gatgaccgcg acgggccgtg agctgaaggg cgccgagctc
      421 gccgcggcgc agcagctata tgccgcgaag tttcctgatg catcggattc cgaagcggcc
      481 tccggcgcga tcgcgcgcgc cctctcgcgc atgagctggt acgaactcgc ggcggtccag
      541 gtgcgcttca tcgacaacag tcgtggcttc ggacaccgca ccgagtggtc acgcgcgcaa
      601 ttcctggcaa cggcacgtcc tgaatagaac cgggtgctcc gatgcccggt ctcgatgaaa
      661 acatatatgc ataacaggca tatcaaatcg gtctgcgatg ctgcttgact atgatacgag
      721 cggctggtga tatcgaagct ccaatccagc aaagccgacg agcatcaaag gagcgaggat
      781 ggcgagcctt gcgagtgtca agagttggga tgacgaggcc gatgtggtta tcgcggggtc
      841 gggcgtagcc ggctgcgccg cggcgatcga ggcgcacgac cgcgatccca aagcagacat
      901 cctgatgatc gagaagatgc cggaagtgct gcgcggcggg aacagccgcg tctcgggcca
      961 aacgttactg atctcgaaga acgtcgaggc gctcatcagc tatcagcgga acatgagccg
     1021 ctcgaacccc attcccgaag acatgctgcg gagctgggcg gagcagctgg tcgcgctcga
     1081 gccgtacatc gaggagcggg cgaaggaggc gggcaaccgc tatgtgcatg gcggtggctg
     1141 gtgcgatgac cagaccgtca acgagttccc ggagcttggc gcggaggccg cagtcgagcg
     1201 cgtcgcgacg atcctgccgg tgccgagcgg cacctggctc acctttgaca agtgcgtggc
     1261 gttgcgtcct cgcgtccgcc gtatctttga atgtgcgctc gtcgacctcg tgcaggatcc
     1321 cgacacgctc gaggtgctcg gcgtcatcgt cgagtagggc ggcaagcgcc gcgcgatccg
     1381 cgctcgccgc ggggtagtga tggcgatcgg cggcttcgag aacaacatgc agatgctgcg
     1441 agactacaac ggtatgacgg acgtgctgcc attcggcacg cccggcaaca cgggcgacgg
     1501 catcaagatc ctgcagaagg caggagcgga catgtggcac ctgcgcaatc gtgtccagtc
     1561 ctccggcatc tggccgggct ttcgctatcc cggcaggaac acggtgttca agcgtcacct
     1621 gttcttccag gccttcagct ggatagacat cgcccaggac aacacgcgct ttgtcaacga
     1681 gaccgcagag taccggctca accactacaa ggtgagggag catggcgagt ggatcgatcc
     1741 gccgcaccag cgcgtgggtc gaatgcacat gattttcgac gaggcgacgc ggcagggaac
     1801 ctgcctggtc atcggaatca tgacctggaa ctcgatagtg gaaggctacg agtggagcga
     1861 ggacaattcg aaggaagtcg ccgccggctg gatcgtgaag gcggactcca tcgaagagct
     1921 cgcgcggaag atcgatcgcg acccggcgag cgtggccgcg acggtgcgcg agtacaacga
     1981 agcgtgcgcg gccggtcacg acgcgaaatt cggccgcgag ccgcagaccc tgcagccgat
     2041 cgcgaaaccg ccgtactacg cgatcgaggt cgtcccctcg atcgtgtgca ccggcggtgg
     2101 agcccggcgc aacatcgagt cggaggtgct cgatcacggc ggcaagcgga ttccgcgcct
     2161 gtacgaggct ggcgagctgg gttcgatgtt ctccgatctg taccagaacg gctcgtatct
     2221 gaccgaagcg atgatctcgg gccgagccgc aggacgcaac gcggtacagc agaagaactg
     2281 ggcgacgtga agtaatcgga gcctgagccg gggagccccg cctgatcggg cgcggggcaa
     2341 gcggcgcgcc gtgcaaccgc aggcgccggg caggatggcg atcaaccagg tgaatacgcc
     2401 ggtgatgcgg acagtagggc aattgcagac ggttcacttc ggccgcgagc agcgggtgat
     2461 ggaaggcgtg gtcacatgct ggtgaagacg cccgccatgc gcatcgatgt gcacgtggag
     2521 tccgtgaagg tggatgggaa acatctgctt ctcgcggggc tcgccgggca cctcccctgc
     2581 gagatgcgag ccggggctcc cgaactgcgc gcgctgctgc gcctcgcgct gaagccacgc
     2641 atcctgttat ggctgctgcg cggtaagacc tgagttctcg accatggcgc cggcacgcgg
     2701 gcgacaagcg gccgtagcgg gccgcgagct cgccggggtg tgtcaggcga gcgcggcgct
     2761 cggcgtctcg tggtcgtcgt cgctgacctc gacggcgaca tcgtgcacgc gagccggcgc
     2821 gcccgcactg gcacggccgg catccgggcc cgcggcctgc gccagcgcca cctgcaactc
     2881 ctcggcgctc gcgtagcgtc gcgccggagt cttcgcgagc agcctgtcga tcagcggctg
     2941 caggtgggca tgctccgccg gcagcaacgg caccggcgcc gtgacatggc gctgcaacac
     3001 ctcgatcgcg ctcgagccgg tgaacggctt gcggccggtc agcatctcga acagcacgac
     3061 gcccaggctg tacaggtcgc tgcgcgcatc gagcggcagg ccctgcgcct gctcggggct
     3121 catgtaataa ggcgagccgc gcagcatgcc ggtgctcgtg gtcgtgatct cggcatgcat
     3181 ctggcgcgca agtccgaagt cgatcagcac cacctcgtcg ttctcgcgca gcatgatgtt
     3241 gggcggcttc aggtcgcgat gcacgaggcc ggcggcgtgc accaccccga gcgcgcgcgc
     3301 gatgcggcgc acgtaatcga gcgcatcgcc ggcatccatc gggtgctgca ggcgcgcctt
     3361 caggtcgccg cgcgggaaat actccatcgc gaggtactcg aggccgtcgt gcacgccgta
     3421 gtcgtagatg tcgatcaccg acggatcgtg gatcgaggag atcacttcat attcacgcgc
     3481 gaagtcctgg cgcaccgtgc gttcgcgggg cgcgccatcg accgcgggga ggctgtcgtc
     3541 accggtgcgc gtcaccttga gcgcgaccag tgagtcgagc tcgaagctgg tcgcgagata
     3601 cacgaccgcc cgatcggact cgccgagcac gtcgaggatg gcgtaacgcg ggatcaggct
     3661 gcgcggcagg cgggcacgcc cgccgggtgg aacgctccgc ggcgcgggcg ccggttcgct
     3721 cttgcgtgag cggtccgcgg cgcgcctgcg ctcgactgcg cgcagcgcga tcttgatggc
     3781 ggtattgagc cggctcggag tcagaagatg cttcggcagg tagtcgctcg cgccgagacg
     3841 gatcgcgcgc accgcggtga gttcattgcc gcctgccgcg atggcgatga tgggaggcag
     3901 cagggtctgg tcccgcaggc gccgcagcca gccgagcccc tcggccgccg catcctcggg
     3961 gctgttgccg aagttcattg cgagcagcag cacgtcccag tcctggaagc gcaccgtggc
     4021 gcgtcgctgc tcgaactcgg ggatgtcgac gaactcgacc tgcgcgtccg gcagcacggc
     4081 gccgacatgc gccgtcagcc acgcgccgta gcgggcgtcg tcgtcgatga tcatgacgcg
     4141 gcagggcacg ggactgccgt ttactgcaga tcgaagcgca tgcgcagctt cgcgcgctgc
     4201 ggcacggcga cgccgttggc ttcgacgggc cggaagcgcc agcgcgacag cgcatcgctc
     4261 gccgcttcgt cgaacacacc ggccgggctc gacccggtga cttgcacatc ttccacggtc
     4321 ccggtaggcg tgaccgtgaa ctcgaggtcg acccagccct cctggcccgc ggccagcgcg
     4381 gcggcgggat aacgcggctc gagcatgcgc gtgcgggtca gctggctcac cggcacggct
     4441 tcgttcaggc gctgggcgcg ggtccgctgg gtggcgatgt cgcgcttcgc attagtgagc
     4501 tcgaggcccg taaagccgat ggcctcggcc tcgccgaccc agcgctccgc gccggcgata
     4561 tcgccgcgcg cgagcgcggc gccggattcg gccagcatcc tgccgccgag cgcattgcgc
     4621 gcgttcaggg tctggggatg ctgcgcattc gcctcgcgca tctgcgcata gagcgcgagt
     4681 gcgctgtcca cggcgggctc gagcagccgg ttggcgcgca gctgtgcatt gaatcgctgc
     4741 ccgagcgcgg tgaaggcgtc cgcctcgagc gcgaccctgg cggcggcgag ttcgcttcgc
     4801 agcgcatcga gttcgccatg cgcgacgcca ttgtccagag cggccgtgat gcggagttcc
     4861 gccgccgcga tatctcccgc gctgatcgcg gcgcgcgcat cgtcgaccag cgcggcctgc
     4921 agtttccgca gcgcctcgcg cagtccggcg tgttcgggcg agagcgcacg cgctgattcg
     4981 atgtagtagc gggcattgtc ctgtgccggc gcaagcaacg cgccctcccg cacgcgcgcg
     5041 tcggcgagcg cgaggaaatt ggcggcctgc aggtcgatct cctgctgctg cagttcgcgc
     5101 ctggcagcgc cgatcagctc ggacccgccg gacgatccgc tctcgagcac cgcgatcgcc
     5161 cggtcgaggt tgccgctggc ggcggcttgg cgcgctacgg tgagcagttg gcgctcgcgc
     5221 tccttgccga tctgcgtcgc gagaaaggcg acacgcggat gatccggcag cacggcgcgc
     5281 gcgacatcga ccagccgcgt cgcctcatcg agttcctcgg cgacgattgc ctgctcggcc
     5341 tgcgcgagat ggcgattggc gatgtcatcg agcccggcag tggcgcgcgt gtgtccgggc
     5401 gatttcgcga gcacgagctt gtacaggtcc gccgctgact ccccgggagg cgatacgagc
     5461 tggccggagg cgagcgcggc gtcggcacgt tcgagcagcc gttgcgtggc gggatcgaga
     5521 tccgaaggcg cggcctgcgc gggctgcgcg ttcgcgggcg cgctgctgcg tggcgcggcg
     5581 gctggtgcgt cgaccgcgac cgcggctggc ggcgcttcgc gcgtcgtcca ccagatcgcc
     5641 gcgcccgcca cgattgcgag cgcaagggcg ccgagcacga gcggcgtgcg cgagcgggcg
     5701 ccggaggagt gcgtgggcgg gctggccgtt ggtgccgctg gcagtgcgcc gctgtggcgg
     5761 aacgcggatt cgacgaacag cttcacgcgc tgcgcggaga cgggcttgtg caggaagcgg
     5821 aacacgcgcc cgtcggtgat ctgcggcgcg agcacgccct gctcggcgcc cgtgccggcg
     5881 accaccagca cgagatccgg gaactgtgcc ttgagtcgcg cggtgagttc ggcgaccggc
     5941 acgctggccg cggcgctgtc gatcagcgcc gcgcgcgtct ggctcgccat caggtgatcg
     6001 ctgagcgcgg tgtcggaggc gacgaattcg aagcgatgtc cgtcctcgac gagcacgcgc
     6061 agggcggcca ccagcgcagg gtcgcgcgtg agcgcgacga ggcccgccgg cgcaccgaca
     6121 ggagcgaatc ccgactgcaa agcggtcgcc tccctgcttg cttgttctgc ggtcctgctg
     6181 gccacggcgc gctccaatcc aatgccgcgg atttacaggg gattcaggca agatactgtg
     6241 atcgccgtcg tcgccgcgat agcccgccga cgggcgacgc gggggacccg acgaaaggcg
     6301 gcggaaacgc gaaccgcgtc acagttcggg cgagcggcta tgatcgcggt atggtcagga
     6361 acgtgcgcat agtcactctt ctggtcctcg cgctcatcgc gccggcggcc tggtcggcag
     6421 gcgtcagcgg tgcgcaggag cgcgccgccc aggagttcct cgcggcggtc gcatccggca
     6481 acacccaggc catcgctgcg agcctgcatc ccgatgaact gcgccgcgtg cgcgccggca
     6541 tcaccgcgag actgcgcgcg gatgccgcgg tcggcgatca ccgcgtgcgc agccggctgt
     6601 tcggcgaggc gtcgaacctc ggcgacatcg agcgcctgac cgaccagtcc ttcttcgcgg
     6661 ccttcggcaa gcgcctgcgc tatccgggcc gcctgttcgc gaaggtcaag ggcctcgagg
     6721 cggtcaagga cggcgacgac atcgtgcata tcgtgattcg cggcgaacag ccggaaggcc
     6781 gtggcaagat gcgcgtggtc acgctcgtga ccctgatggc ggagggcagg ggatggaagg
     6841 ccgccatgcc gagcgaggtc gaggcgcaga tcgaggacct gctcgacggc cgtgaacccg
     6901 ccaccggact cgtgcccctg gcacccgtcg cgagcgcggc gcccggcgcc gcgcaggccg
     6961 tcaattcacc cgagatccgc agcctgctct cgagcgccgc cggcgcgctg ctcggcggcc
     7021 attgcgacga gtactacggc gagtacatga gcccgaattt ccgccgttcc acctccgcgc
     7081 gcgcgctcgg tacgctggtg aagtcctgcg aacgtagcga ttcgatgcgc gaaatgctgg
     7141 tggccacgct gcgtatcgtc gatggctcct cgccgcgcta cgaacaggac ggcgcgcgtg
     7201 cgcgctacga catcaccggt cgcggcctgc cgtacgacca cttcgtgctc gagaagatcg
     7261 acggacgctg gtacatcgcg gaatgagtga gccccgcatt cgtccggcgc tgcccgccga
     7321 tgcgcccgta ctgctgtcgc tgatccgcgg cctcgccgag tacgaaaagc tggcgcacga
     7381 agtggtcgcc accgaagcgc agctcgccgc cgaactcggc gcgcccgacc cggtggtgca
     7441 tgctttgatt gcggagcggg cgggcgagcc ggtgggcttt gcactctact tcttcagttt
     7501 ctcgaccttt ctcggccgcc gggggctgta cctcgaggat ctcttcgtgc tgcccgcgca
     7561 gcgcggccac ggcatcggcc gtgcgttgct ggccgagctg gcaggccgcg cagccgagcg
     7621 gggctgtggc cgtatggaat ggtcggtcct cgactggaat gtcgatgcgc agcgcttcta
     7681 tgccggcctc ggcgcacgcc ccctgtcagg gtggaccgtg taccggctgg atcgcgcggc
     7741 catcgcggcg ctgggttagg atgtcaaatc aagctgaagg agaccctccc atgtcagtag
     7801 ccaaggtgag tgagatcatt gccagctcga cgaagtcctt cgacgatgcg atcgccacgg
     7861 gcgtgaagcg cgcgacgaag accctgacgg gcgtccagtc ggcctgggtt gcggaccagg
     7921 aagtgcacgt caagaacagc aagatcatcg aataccgcgt gcggctgaag gtcaccttcg
     7981 tcctgaagta ggcgccgcgt actcctgcac gccgaccacc agcatgcccg gcccgccatg
     8041 cacgccgagg gcggtgccga gcggcgtgag gtgcgcggat tcgacctgcg gcacgcagct
     8101 gcgcagggcg tcgagcagtc gctcgccctc gcgctgcgcg tccgcgtggc ccaccgtgat
     8161 ccggtaggcc acgccgcgac gcaggcggcg cgccacgaag cgggcgaagc gcggcaccgg
     8221 gtcgccgcga ccgaacagcg cgccgcgcgg cttgacccgc ccgtccggca ggttcgtgag
     8281 caccggcgag cagcgcagcg cgcgtgacag cttctgcact agcggcgtca cacgcccgcc
     8341 gcgcaccgcg taatcgagtc gcacgagcag ggcccaggtg cgggtcttcg cgcgcatcga
     8401 ttccaccgcg gcaaccagct gcgcgccgtc gtagcccgcg agcgcgcact ccgccgcgtg
     8461 tatcgccagc agcccctggc cgagtgaagc gttcaggctg tcgatcacgg tcacctttcc
     8521 gccggcaacg cgtgtcgccg cgctgcttgc cgccgcgtgg gtgccgctca cccggccgct
     8581 cacggtgatc gacacgacgt gcgcatagtg cgaggcgagg aactcgtaca tgcgccggaa
     8641 gtcgcccggc ggcggctgcg aggtctgcgg atgaaccccg cccgtcgcca gttcgcgata
     8701 gaactcctgc ggcgacagcg acaccttgtc gagatagctg cgtgcgccga agtgcacgcg
     8761 cagcggcacg atgtgcacgt cgagcttctc gagcagctcc tcgggaatgt cggcgcccga
     8821 atcgctgaca atcgccacgc gcgccgcccc ggtgcgatgc agggcgtggg cctgacgatg
     8881 catgtcgtcg gccttctgcc ccgagagtgc gccgaaatgt gccgccacgt cgaagacccg
     8941 ggccggctcg ttggtgtgga tgtggatacg cgctttgctg cgcgtgccgg cgatcacgag
     9001 gctcgagccg agcgtggtga gttcctcgcg caggcgccgc tggtcgacgc cctcgccact
     9061 gaccatgcat tcggtgcagt agcggtgcgc accctcggca ccgcctgccg acatggcttc
     9121 caccgcatcg tgcggcggtg tgaccgcggc gccgatctcg ccggtctcga gatacgatga
     9181 catgccctcg acgatctcga tgaagcccgc cgcgccggcg tcgacgacat tggccgcgcg
     9241 caatacgctg agggagccgg tcgtggccgc gagcgccgcg cgcagcgcgg gcagcgtctc
     9301 gcgcagggat tgcccgacgc cgatggggcc gccggcggcg acccggcgca gctcggcggc
     9361 gagcgcggcc agcacactga gcagcgtgcc ttcctgcggt tcggtcaccg cgtcgcgcgc
     9421 atagcgcgca ccggtgtcgg tcgcggccgc gaactcggtg ccgccgatcg tggcgagcgg
     9481 cccgacacga tcggcgacgc cgtgcaggaa ctgcgcgagg atcgcgccgg agttgccgcg
     9541 tgcgccgtcg atcgcggcgt cggcgacccg cgccagcagc tcgccggcgt gcgcggtgcg
     9601 ctgcgtgtcg aggcgcgcga gcaccgcgga catcgtcatc gccatgttgg tgcccgtgtc
     9661 gccgtcgggc accgggaaca cattgatgct gttcaggtga tcggtgcgcg cgaacagccg
     9721 gtagatgccg gcacgcagcg cggcggcgag cgactcgccg tcgagcgggt cgcgcccggg
     9781 cacctgggtg cgtgggtcag tgctgctcaa gctggaagcg cgtgccgcgg aacgacatca
     9841 cggcgcccga gggcgtgatc tcctcgaggc gtatgccctc cggcgtggca tcgccctctc
     9901 gcagccgccg catgttgatc atgacgaagc ggtcggcggc gcgggtcgca tacacgtgca
     9961 ggtcgaggcg caggggcggc agctggttgg cgatcgcggg cggcagttgc tcgagggttg
    10021 gcaccatgcc gcgctccgct gcctgcgtgg ctgcgtcggg cgccgggccg acgggcagcg
    10081 cctgttcgta gtcggcgggg ttgccgctgt agtccggcgg ggcataggcg gcatcgtcct
    10141 gcgccagggc ggcgcccgcc gagaggtcgg gcgcgggggc ggccacttgc ggtggcagca
    10201 cctgtgcggt gccagcctgc ggcgccacgc ctggcggcga gcccgccggc accggcgtgc
    10261 cggtgggcgt tggcgtgagg gtggcagcgg ctggcgtgtc agccgccggg gcgtcggcac
    10321 gtggcgcggt ggcgggcgcc ccttgctgcg ccggcgccgt ctgcggctcg cgcatcatga
    10381 accacgcgag cacgacgaga ttgaccagca gcagcgcgcc gagcgcgatc gcccagaacg
    10441 gcaacccgcc gcgcggcggc gccttgcgga tctcgaacag cgccggtccg ctctggcgct
    10501 ggcgctcgct gtcggatttc ttgagcgcgt cgaggatgaa cgacaccgat cagccctccc
    10561 cggcggaggc ggtgatgagc cgcggcgtac cggcggccgg catcgcggcg tcgagtgcga
    10621 gctgcgtctg caggccggca atgccgtcga cgtgcaggcg gcgcgatcgc tggaaatcct
    10681 ccacgagccg cacgagcgac gcatcgtagc gatcgctcga cgcatctgcg gggctcaagc
    10741 ccgcgacctg ctcgaggctc cgccgaagct gcagcacgct gtcgccggac atgcccggcg
    10801 cgagcgccct gaccggcaat gcaatcgggc gccacaacac tacgaagtcg ccgaaccagt
    10861 aacgggcgag gtcggcgatg ccgaccgtgt gctcggcgcc gccgatgtcg agtcgcgccg
    10921 tgtcgccatc gagcgcggtg agcacgacct ggtgcgcgcc gccctgttcg tcaccgagca
    10981 gcagcaacgc gggacggtcg tgaacccgca gctgcgcgaa cgagccgcgc tgcgcgacgc
    11041 attgcaggcc ggcgagttcc gcctgcgcgc aggcatcgcc cgcgccgggt gtgtaggtga
    11101 cgttccagcg agcgagcagg cgggagtagg cggcgtcgct gccggttgcg ctgcctgcac
    11161 cggcgagcag cgggccgagt gtcggcaggg acggcacgga cggcgcgggc ggtgcgggtg
    11221 gctcggccgc gaccgacgct tccgggattg ccgcgaccga gcccgacgcc ccggagagcg
    11281 cgccggctgc atcccgcgcg ccacgatcga gcacaccgga ctgccacagc gccacggcgg
    11341 cgaggatcgt caccagcgcg cctccggcgc ccgcggccca gggcagccag cccggcgcga
    11401 tgcgccgacc gaagacttca ctcgccgagt tgcgcacgat cgtcgcggtg acacgatggc
    11461 ggtcctgcgt gtatgcgccg agcagcgcgc gatcgcagat cacattgatg agccggggaa
    11521 tgccgcccga ttgccggtag acctcggcga gtgcgccgct cgtgaagatc tcggtggtgg
    11581 cgcccgccac gcgcaggcgg tgcagcacat aggccgacgc ttcggccctg gacaacgggt
    11641 cgaggtgata gcggccggtg atgcgctggg cgagctggcg cagttcggcg cgcgccagca
    11701 tttcgcgcag ttcgggctgg ccgatcagga tgatctgcag cagcttctgc gtcgccgtct
    11761 cgaggttggt cagcagtcgc acctgctcga gcacggtcgc gtcgaggttc tgcgcttcat
    11821 cgacgagcag cacgacccgt cgcccctcgg catgcgcgcg cagcaggtag tggttcagga
    11881 tgtcgacgag atccttgtgg ctcgccactg cccggtcggg caggccgatg ccgagctcct
    11941 cgcagatcgt cagcatgaac tcgggcgccg tcatgcgcgg attcaggatc agcgcgacct
    12001 cggcgttctt cgggatctgg ccgagcagcg agcgcactat tgtcgtcttg ccggtgccga
    12061 cctcgccggt cagttgcacg aagccgccgg cttcgctgac tccgtacagc aggtgcgcga
    12121 gcgcctccgc gtggcgctcg ctcaggtaga ggtagcgcgg atcgggggtg atcgagaacg
    12181 gcttctcgtg gagtccaaag aacgacaggt acatcgcgcg ggtctgtcgc cagctgccca
    12241 gaactgccgc cgagtatacg cgggtggcgg ccgcatcagc gccggcgccg ctcctcctcg
    12301 tacagcgcca gcttcgagtg cttgccgacc ggggtcttcg gcagctcggc gcgcagctcg
    12361 agcgcgcgca ccatctcgtg gcggccgacg cgacccgaga ggaactcctg cagcgcctcg
    12421 agcgtgagct ccggcgcgcc gtccttcagc ctgatgaagg ccttcggcga ctggccgcgg
    12481 tactcgtccg cgacgccgat cacgcacacc tcggcgacgt cggggtgggc gtagatcgca
    12541 tcttcgatca cgcgcggata gacgttgtag ccgctacaga gcagcatgtc cttgcagcgc
    12601 tcgatgatga atacgcagcc gtcgccatcg atgtagccga cgtctccggt gcggaaccag
    12661 ccatcgggcg taaacgccgc ggcggtgtcc tgcggctgct gccagtagcc gcgcatcacg
    12721 ttcggcccgc ggatgcagat ctctccgagc tcgcccggcg cgagctcgcg cccggcatcg
    12781 tcggtcgcga cgatcttcat gtcgacgcgc ggcagcggca ggccgcagga gccgggcttc
    12841 tgcaggccgt ggcgcggcgt gaaggtaccg gcggcggtgg tctcgctcat gccccagccg
    12901 tcggtgacct gcacgccggt gagctggcgg aactgctcga cgatctcgag cgcgagcggc
    12961 gcgccgccgg agccgcagtg tttcagcgac gaaaggtcga tcggatgcgc gcgcgcatgc
    13021 gcggcgagcg cgaggaacat cgtcggcacg ccgaacatca cggtgatgcg tctcgccgca
    13081 atgtcgttgg ccacggcggc gacatcgaac ttcgggtgca gcacgagttc ggtgccgagg
    13141 ctgatgccga ggaacatgat gagttcggcg tagatgtgga acagcggcag cacgagcagc
    13201 atccgctcct cgcccggcag catcgagcgc ggctcggcgc gggtcgtctc gacgcactgc
    13261 gcggcggcca tggagatgtt gccgtgggtc agcgaggcgc ccttcggtac gccggtggta
    13321 ccgccggtgt actgcagaac cgcgatgtgg gtcgccggat cgtcgatcgg ctgcggcgtg
    13381 tagcggccgt cgttgtcgag cagctcgagg aagcgcaggt gcgcggcgtc gcgcggtacc
    13441 tcgcactgcg gcgggatcgg cacacctgcg aaatcggcga ggctgccgac gatgacggtc
    13501 ttgaggcgcg tgcccgcgag caggctctcg gccttcggca gcagcacctc gaggtcgagg
    13561 gtgaccagca cgtcggtgcc gctgtcctcg actttgcgcg cgagcgtggc ctcggcatcg
    13621 aggggcgaat agttgacgac gatcgcgccg gccttgagcg caccgaagaa ggcgatcgga
    13681 tactgcgggc aattcggcag atagagtccg acgcgatcgc ccggccgcac gcccaggcgc
    13741 tgcaggcccg cggcggcgcg atccaccagc gcgtgcagct cgcgatagct gagcttgcgc
    13801 gcaaagaagg tgagcgcggg atgatcgggc cagctcgcga cggccgtgtc gagcatccgc
    13861 caggccggtg cgggctggtc atcgatgtcc cagcgcacat cggccgggta tgactcgagc
    13921 cacggccgcg ccatggccta gacgacttcg aacaggcctg ccgcgcccat gccgccgccg
    13981 atgcacatgg tcacgacgac atacttcacg ccgcgacgct tgccctcgat cagcgcgtgc
    14041 cccgcgaggc gcgtgccgga gacgccgtac gggtggccga cggcgatcgc gccgccgttg
    14101 acgttcaggc gctcgtcggg aatgccgagc ctgtcgcggc agtagatgac ctgcaccgcg
    14161 aacgcctcgt tgagttccca cagtccgatg tcgtcgatac ccaggcccgc gcgctcgagc
    14221 aggcgcggca ccgcgaacac cggcccgatg cccatttcgt cgggctcgca gccggccatg
    14281 gtgaagccgc ggaaaatgcc gagcggctgg atgccccgct tctcggcgag cgtggcatcc
    14341 atgaccacgc aggccgcggc accgtccgag aactggctcg cgttgcccgc ggcgatcacg
    14401 ccgccgggga tcgccgggcg gatcttcgag acgccctcga aggtggtgtc ggggcggatg
    14461 ccctcgtcct tcgccacggt cacctcgcgc gtgccgagct gctgcgtctt cgggtccgca
    14521 tagcccgcgg tcaccgtgat cggcgcgatc tccgcatcga acagcccggc cgcctgcgcc
    14581 gcggccgcgc gctgctggct gcgcgcccca tactcgtcct gccgctcgcg ggagatcgag
    14641 tagcgcctgg ccacgttctc cgccgtctgc agcatcggca ggtagagatc gggcttgtgt
    14701 tccgcgagcc agggttcgcg cagcatgtgc gtgttcatct cgttctgcac gcacgagatc
    14761 gcctcgacgc cgcccgcgac cacgatcggc gtcttctcga cgatgacgcg cgcggcggcc
    14821 atcgcgatgc tctgcagccc cgaggagcag aagcggttga tcgtgacgcc gccggtcgtc
    14881 accgggcaac cggcgcgcag cgccgactgt cgcgcgacgt tcatgccggt ggcgccttcc
    14941 ggataggcgc agcccatcag cacgtcctcg acctccgccg ggtcgatgcc ggcgcgcgcg
    15001 atcgcgtgct gcacgacatg gccgccgagc gtggcgccgt gcgtgatgtt gaaactgccg
    15061 cgccaggact tggcgagcgg cgtgcgggca atcgaaacga taacggcgtc ggtcattgcg
    15121 gtatccccgg aatcagctga atgtcttgcc ggcggcggcc agcttcgcca gcgagcgcgc
    15181 gggctcccag gcgagcggat caccgtgcgg attgcgcgcg aactgcttca tgcgccgcac
    15241 gaccacgtcg aggccgacgg tgtcggcgta gagcatcggc ccgccgcggt gcggcgggaa
    15301 gccatagccg ttcaggtaaa ccatgtcgat gtcggacgcg cgcagcgcga tgccttcctc
    15361 gaggatcttc gcgccttcgt tgacgagcgc gtacacgacg cgatccacga tctcctcatc
    15421 gctgatcgca cggcgcttca cgccgatttc cttcgagtaa tcgacgatca tctgctcgac
    15481 ggcggccgac ggtcgcgcgg tccgatcgcc ctccttgtag tcgtaccagc cggcgcccgt
    15541 cttctgtccg aagcggccct gctcgcagag ccggtcggcg acgcgcgaat aggtgaccag
    15601 cggcttctcg acgtagcggc gtttgcggat cgcccagccg atgtcgttgc cggcgaggtc
    15661 ggcgacacgg aacggcccca tcgcgaagcc gaacttctcc atcgcggcgt cgacctgctt
    15721 cggcagcgcg ccttcctcga gcaggaacag ggtctggcgc cccatctgct cgagcatgcg
    15781 gttgccgatg aagccgtcgc agactcccga gaccaccccg atcttgcgca tcttcttcgc
    15841 gagctgcatc accgtcgcga gcacgtcggg cgcggtcttc gcgccgcgca ccacttccag
    15901 caggcgcatc acattggccg gactgaagaa gtgggtgccg atcacgtcgc cgggccggtt
    15961 ggtgaacccg gcgatgcggt tcagatcgag cgtggaggtg ttcgaggcaa ggattgcgcc
    16021 cggcttcatc accgcatcga gcttctcgaa cacggcctgc ttgacggaca tctcctcgaa
    16081 caccgcttcg atgacgatgt cggcctgcgc gatcgccgcg tagtcgagcg ccggcgtgat
    16141 cagctgcatg cgcgattcca cgtcggcttc cttcatgcga cccttcttca ccgcgccgcc
    16201 gtagttgcgg cggatcgtct cgaggccgcg atcgagcgct tcctgcttca tctcgagcag
    16261 cgtgaccggg atgcccgcat tgatgaagtt catggtgatg ccgccgccca tcgtgccggc
    16321 gccgatcacc gccgcgcgcg caatcgggcg ggtcggtgtc gagtcgggca catcggggat
    16381 cttcgcggcc gcgcgttcgc cgaagaagat gtggcgcgcc gccttcgact ccgggctctg
    16441 catcagttcg ctgaacagcc gccgctcggt cgccatgccg gtttcgaacg gggtggtggc
    16501 cgccgcaagg accgcctcga cgcacttgcg tggcgcgggc cacgggccgg ccacgctctc
    16561 gacggtattg cgcgcaaaca tcaggaagcc ctcgaggttc gggtgcttca ccgcgagatc
    16621 gcgcacgcgt ttcgccggac gcttctcctt gatgaggcgc gtggcgtagt cgatcgcgcc
    16681 cgcgcgcagg tcgccctcga gaatatcgtc gagcagggcg ctgccggcga atgccttcgc
    16741 gggcaccacg ctgccggaga cgatcatgtt gagcgcggcc tcgagaccga gcgcgcgcgg
    16801 caggcgctgg gtgccgccgg cgcccggcag caggcccagc ttcacctcgg gcagcgcgat
    16861 ctgcgcatcg ggcagggcga cccggtagtg gcaggcgagc gcgagctcga ggccgccgcc
    16921 catgcacgag ccgtggatgg ccgcgactac cggcttggtg ctcgcctcga acgcgctgag
    16981 cagggtcagc aggttcggcg gcgcgtacga cttcggcgta ttgaactcgc ggatatcggc
    17041 gcccgccgaa aacgccttgc ccgatccgat gagcacgatc gccttcacgc cacgcgcggc
    17101 gagtgccttg tcgagcgatt tcagcagcgc ggcgcgcaat ggctgcccga gaccgttgac
    17161 gggcggactg tccatggtga tcacggcgac cttgccgtgc ttctcgtatg tggtcacacc
    17221 catgcctcga tactccccgt cctgcaatct gccgaagccg tccagcctaa gggttgtacc
    17281 ccgccgggat caaattttcg gggatgcaat ctgtgcatga atgaccatgt gcaatattta
    17341 tttgcgagat gaaccgcccg cttctaccct acccggaggg catcgggagg ggcgtcgcgc
    17401 cagcgacaca tggaactcgc attctcggct gaggacgagg cttttcggag cgaagtgcgc
    17461 gagttcgtgc gtgccaagct gccgttgggc ctgcgcaaca aggttgtcgc gggggagcgc
    17521 ctcgcgaaat cggaatacct cacctggcat cgcatcctgt tcgaacgcgg ctggatcgca
    17581 cccaactggc ccgcagcctt cggcggttgc gaatggagcg cgattcgcca gcacatcttc
    17641 gacgaggaat gcgcgctcgc cggcgccccc ggcatcctgc ccttcggcat ccgcatggtc
    17701 gcgccggtga tcatgaagtt cggcaacgcc gcgcaacagc agcggttcct gccgcgcatc
    17761 ctgtcgggcg aggactggtg gtgccagggc tattccgagc ccggcgccgg ctccgacctc
    17821 gcctcgctca agatgcgcgc ggagcggcgc ggcgggcact acctcgtcaa tggccagaag
    17881 acctggacca cgctcgcgca ctacgccgac tggatcttct gcctggtgcg cacttccacc
    17941 gaaggacgcc agcaggaggg tatttccttc ctgctggtcg acatgcagag ccgcggcgtc
    18001 agcgtgcgcc cgatcatcat gctcgacggc gggcacgaga taaacgaagt ctggttcgag
    18061 gacgtcgagg tcccggcgga aaacctcgtc ggcgaggaag acaagggctg gacctacgcc
    18121 aaatacctgc tcggccacga gcgcaccggc aatgccggcg tcggccactg ccgccgctgg
    18181 ctcggcgagc tgaagtcact ggcgcggcgc gagcgcagca acgacgggcg cccgctcgtc
    18241 gacgacccgc gctttcgcga ccgcatcgcg cgcctcgaga tcgacctgat ggcgctcgag
    18301 gtgataaacg tacgcgtgct gatgctcgag cagcagcgcc gcgcgcccgg acccgaggct
    18361 tcgatgctca agatcatggg cagcgacctg cagcaggcgc tgaccgagga gacgatgcgc
    18421 gcctcgggcc acggcgcgat gcgctatccg cagccgggca ccggccatta cttcaacttc
    18481 cgcaaggcgt cgatctacgc ggggtccaac gagatccagc gcaacatcat tgcgcgccac
    18541 atcctcgggt tctgaccttg atcactctca ccgacgagca gcagcagttc cgcgacagcc
    18601 tgcagcgcat gctgcgcacc gactacagct tcgagcgccg gcgcgaaatc gccgcgagcg
    18661 acagcggctg gagcgaggcg acctggagcg ggctcgcgga gctcggcgcg ctcgcgctgc
    18721 cgctgcccga ggcgcatggc ggccttggcg gcggcgcgat ggacgtgctc gtcgtgatgg
    18781 aagagtgcgg ccgcgcactg ctgctcgagc cgtacctcgc caccgtcgtg atgggcgcgg
    18841 ggctcgtcgc gcgcatcggc agcgatgcac agcgcgcggc catcctgccc gccgtggccg
    18901 ccggcgaatg ccgtctcgcg ctggcgcact acgaatccgc cggtcgctac gcgctcgatg
    18961 cggtcgccac cgaggcgcgc cccgggggcg cgggctggat cctcgcgggc gagaagcact
    19021 ctgtgctgca cggcgcggcc gccgaccgcc tgctcgtgtc ggcgaggacg ccgcgcggcc
    19081 tgtcgctctt cctcgtcgag cgcggggcgc cgggtctcgc ggtgagcgac tacccgcagt
    19141 acgacgggca gcgggcggcc acgatccgcc tcgaccaggt acgggtcgac gccggggcgc
    19201 tgctcggcgg ggagggcgcc gccgccgagg cgatcgagta cacgatcgac gcggcgatcg
    19261 cggcggtgtg cgccgaggcc gtcggggcga tgggcgcgct gctggaggcg accggccagt
    19321 acctcaagac ccgcaggcag ttcggcgtgc cgatcggcag cttccaggta ctgcagcacc
    19381 ggatggcgga catgctgctc gagaccgaaa aggcgcgctc gatggcgctg ctcgccgcga
    19441 gccgtctggc cgacgccacg cgcgatgagc ggcggcgcat cgtgtcggcc gcgaaggtca
    19501 aggtcggcga ggctgcccgc ctcgtcggcc agcaggccgt gcaactgcac ggcggcatcg
    19561 gcgtgacgga cgaactggcc gtgggccact atttcaaacg cctgacaatg atcgagctcg
    19621 ccttcggcga tatcgattat catctggggc aagtgggcag tcgtatattc gacggaacaa
    19681 gtaccggttg aaccaatcaa ggagtagaga catgcggtca ctacgtgggg tcatgcgcgc
    19741 catcctcggc tgcgcggcgg tgtccctggc ctgcggtggc acagccgtcg cacaggacca
    19801 ggattcggag cggggccgga cactcgacga agtgctggtt accgcgcaga agcgcgagca
    19861 gaacgtgcag gacgtgccga tcgtcgtcac ggcggtgtcg ggccagctgc tgcaggacag
    19921 cggcgtgcac gacatcaagg atctcacgct cctcgtaccg ggcctgatcg tcacctcgac
    19981 cagcaacgag accagcacca cggcgcgcat ccgcggcatg ggcacggtcg gcgacaatat
    20041 cggcctcgag tcctctgtcg gtgtcgtgat cgacgaggtc taccggcccc gcaacggcgt
    20101 cggcttcggc gatctcggcg aactcgagcg catcgaagtg ctgaagggcc cgcagggcac
    20161 gctgttcggc aagaacacgt ccgccggcgt catcaacgtc aagaccaagg cgccgagttt
    20221 cgatgtcgac gccgacgtcg cgctgagcgc cggcaactac ggcctgctcg agcagtcgtt
    20281 cgcgctgacc gggccgatcg ccggcgacaa gctcgccgga cgcatcttcc tcgcccatcg
    20341 cgagtacgac ggcctgctcg atgtcggcac cggcatcggc gggcgcggcg acaccacgga
    20401 catggatcgc gaggtcaaca cggtgcgcgg ccagctgctg tggcggccgg gtgacaactt
    20461 cgaggcgcgc ctgatcgccg actggtcgga gcgcaacgag agctgctgcc tcggcgtgca
    20521 gtacgtgacc ggcccgaccg gccccatcgt cgccgcgctc tcgggcgggg cgatcgccgt
    20581 gcccgccgat ccgttcgagc gcagggccta cgccaatcgc gacacgatcc aggacatccg
    20641 cgaccacggc gcgtcgctgc agatggactg ggacctcgcg ggcggcgcga ccgtgacctc
    20701 ggtcaccgcc tggcgcaact gggagaccga gaacggccag gacagcgact tcagcggtgc
    20761 cggcatcctg ttccgcgatt ccaagaacta cagcaacacg ttcgagaccc tgacccagga
    20821 actgcgcatc gccgggcaga acggccggct cacctggctg gccggcgcct tctacgcgga
    20881 cgaggacctc gaatcgatca atgcgctcaa ctacgacagc cagttcgagc cctacgtgag
    20941 cctgctgctg acccagggtg cgtccacggc gctgctctcg ccgctgctgg gccgcgctcc
    21001 gggcacctcc tacacgctcg gcttcggttc gtacgacacc tatgcccaga gctcgaagag
    21061 ctgggcgctg ttcaccaaca acagcctggc gatcaccgac cagctcgagc tcacgctcgg
    21121 tctgcgctac accgacgagg agaagaagct cacctcgaac tacgacaaca ttggcgacgg
    21181 cgtgggctgc gcggcggtgc gtgcgcgtgc gcccatcttg ccgctgatcc tggccggggc
    21241 gggcattccg gccgcctcgc atgcggcgat catcgcgggc ctcggctcgc tcggttgcgc
    21301 caacttcagc gatccgctgt acaacgactt ctcgcactcg cagaagatct cggagggcga
    21361 gtggagcggc acggccaagc tggcctatcg cttcaccgac gacgccatgg gctacgcctc
    21421 atacgcgcgc ggctacaagg cgagcggctt caacctcgac cgcgagcgca ccagcgtcaa
    21481 tccgtacgag ccgaccgtcg cggtcgacac cgacacgaca ttcgcccccg aggaagtcga
    21541 ttcctgggaa ctcggcgtga agacgacctg ggcgggcaac tcgctggccc tcaatgccgc
    21601 ggcgttctat caggaataca ccggcttcca gctgaacacc tttaccggca ccgtgttcgt
    21661 cgtggcgtcg atcccggagg tcacctcgga gggtgtcgac ttcgacttca cctggtacac
    21721 gccgctcgag agcctgagct tccagggcgg catcacctat gccgacacca agtacggcga
    21781 cttcgtgccc accttcgcga acggccagcg cctgcccggc gcgcgcatgt cgttcgcgcc
    21841 ggagtggtcg ggcaccctgg cggcaacctt cgagcaccac ttcggcgcga acctcaagtg
    21901 gcgcctcaac gtcggcgcga agtacatgtc ggagttcaac accggctccg acctgaaccc
    21961 caccaaggag caggatgcgt tcacgctggt cgatgcccga gtcggcctcg gctcgcgcaa
    22021 cgacagctgg gcgctcgagc tgtggggcag gaacgtgttc gacgaggagt acatccagct
    22081 gggcttcgat gcgaccctgc agccggggac cacgaacgcg ttcctcggca atccgcagat
    22141 gttcggcgcg acgttccgcc tgaagttcta aggggtatgg gagggggcgg cctcggccgc
    22201 cccctcagcc gttgacgacc gaatccatcg ccggcagcgc ctcggccggc gcttgttttt
    22261 ccgctgccgg cgcgttttcg gcgcggccga tgcacggtat cggcccgctg ccatgaaagc
    22321 gctgcccggt atttgcgagc cagcgctcga tgtgacggct gaagcgctcg actgcttcgg
    22381 cgcgcagctt cgcgacgatg tcctcgccgc cgagtttttc ctgcgtggcg ggcagttgcc
    22441 accaccactg caggcgatcg agccgttcgc cgtcgagata cggcatggtg atcgagaatc
    22501 gccggccgga acgcgtgggt gcggtctcgg cgtgggcgaa atagtgttcg agtgcccggg
    22561 gcagttcgac gtgcgccatc gctagactat atcctgtatt gggcgagttc cagcttcccg
    22621 atgtgattgc gatgcacttc gtcgggcccg tccgcgaatc gcagcgcgcg cgcctgtgca
    22681 tacgcatggg cgagaccgaa gtcgtcgctg acgccggccg cgccatggac ctggatcgcc
    22741 cagtcgatca cctggcaggc catcaccggc gcggccacct tgatcatcgc gatttccttg
    22801 gcggcggcct tgttgccgac ggtgtccatc atgtgcgcgg ccttcagcac cagcaggcgc
    22861 gcctggtcga tcaggatgcg cgcatcggcg atgcgctcga gcgtgacggt ctgcgccgcc
    22921 accggcttgc cgaacgccac gcgcgcgagc gcgcgccggc acatcttctc gagcgcccgc
    22981 tcggcaagcc cgatcaggcg catgcaatgg tggatgcggc cggggccgag gcggccctgc
    23041 gcgatcgaga agccgtcgcc ctcgttctgc agcagattcg tggcgggaac ccgcacatcc
    23101 tcgaacagga tctcgccatg gccgtgcggg gcgtcgtcgt agccgaagac gggcagggaa
    23161 cgcaggatcg tgatcccggg cgtctcgcgc ggcaccagga tcatcgattg ctgccggtgg
    23221 cgcgaggggt tctgcgggtc ggtcttgccc atcacgatga gcagcttgca gcgcgggtcg
    23281 gatgcgcccg aggaccacca cttgcggccg ttcacgacat agtcgttgcc gtcgcgcacg
    23341 atcgaggtct cgatgttggt cgcgtcgctc gaggcgacgg taggctcggt catcgcgaag
    23401 caggaacgga tctcgccggc gagcagcggc gcgagccagc ggcgctgctg ctcggcggtg
    23461 ccgtagcgct cgagcacctc catgttgccg gtgtcgggcg ccgagcagtt gaatacttcc
    23521 ggcgaccaga tgacgcggcc catcacttcg cacagcggcg cgtattcgag gttggtgagg
    23581 ccggggccgt gcctcgagtc ggggaggaag aggttccata gcccctcggc gcgcgccttg
    23641 gccttcagct cctcgatgag gcgggtgggc tgccagcgat cgccctcggc gacctgcgcg
    23701 gcgaattccg cctcgttcgg atagacgtgc tcgtccatga aggcctgcag gcgcgcgagc
    23761 agttcgcgcg tacgcgcgtt gtattcgaaa tccatgctta ctctcccgga aggatgacga
    23821 gcttgcccga tacgcgccgc tccacgagcg cgcggatggc ctccccgcca cgcgcgagcg
    23881 gataagtgcc ggcgatgtgc ggtcgcagct tgccggcgcc gagccactcg aagagggtgc
    23941 gaaggtcggc ggcggcccgc gcgggctcgc ggtgcaggta gtcaccgagg aagacgccga
    24001 cgatcgcgca gcccttgagc agcgcgaggt tggcggggat gcgcgggatc tcgccgttcg
    24061 tgaagccgac gatgaggtgt cgcccgcgcc aggcgcagct gcgcagcgcc ggctcggtgt
    24121 agatgccgcc gaccgggtcg tagaccacat cgacgccgcg gccgccggtg agcgccttca
    24181 ggcgctcgcg caggttctcg ttcgaataat cgatcaactc gtcggcgccg tgctcgcggc
    24241 agatcgcgag cttggccgcg ctcgacgccg cggcgatcac gcgcgcgccg agggctttgc
    24301 cgatctcgac cgcggcgagc cccacgccgc ccgaggcgcc gagcaccagc agggtttcgc
    24361 ccgcctgcaa cgcgccccgg tcgacgaggc cgtggtagga ggtggcatag gtcatcacga
    24421 aggccgcacc ggtcggaaaa tcgaggcccg gcggcatcgg cacgagtcgt gccgcatcga
    24481 ccgcgacctg ggtggcgaac gtgccgtaga ccgtgaaggc gatgacccga tctccgaccc
    24541 gggcgttctt cacaccggtg cccacggcgc gaacgacgcc ggcacattcg ccgcccggcg
    24601 caaacggcaa cggcggcttg aactggtatc tgccctcgat gatcagtgag tcgggaaagt
    24661 tgacacccgc ggcgtgcacg tcgatgacga cctggccatc gcccggtacg agatcgggca
    24721 actcctcgac cacgagcgtc tcgggggggc caaattgccg gcagagcagt gctttcatgg
    24781 catgggactc acgggtatgc gaccttcgag gatttcgcgg gcgcgggcct cgatcgacgg
    24841 caggatggcc ggatgggaga tgatgtagaa gcggtccgcc ttcacgccct cgatcgtcaa
    24901 ttgcgcgatg tcggctgcgc tgaggcgtcc ggcgcatatc gcgcggcgca tgcgctcctc
    24961 gaagggcgcg gcgagcggat tggtcgcggc gagttcggcg ggccggttgc gtgccgagtc
    25021 cgcgatgccc gtgttgacga aggccggcgc cagtaccgaa accgcgaggc gcgatttcac
    25081 gagcgcgaga tcgtgcagca ggcattcgga cagcgtgacg accgcgtgct tcgtcgcgca
    25141 gtacaccgcc gagccgggca ccgagatgaa gccggcgacc gaggcggtgt tgacgatgtg
    25201 cccctcctgg ccgcgctcga gcatgcgcgg cacgaagctg cggatgccgt gcacgacacc
    25261 catcagattc acgccgagcg accattgcca gtcctcgatc gtcgcggccc acaccggacc
    25321 gctgacggcg accccggcat tgttgaacag caggtgcacg tcgccgaagg tgtcccagga
    25381 cgccgccgcc agccgttcga ccgaatcggg cttcgacaca tcgcaggtga cggccagcgg
    25441 cgccgccgca ggactcgtgg ggccgcccgc ccgcggcagg agcgcgagcg tggcgtcgag
    25501 gcccgcgcga tcgagatcgg cgagtacgag ccgcatgcct tcgcgcgcac aggcgagcgc
    25561 aagctcgcga ccgatgccac tcgccgcgcc ggtgaccacc gccaccctgc cggtcagctc
    25621 gcgcatcgcg ctcaggccac caggtagccg ccgtcgacgt tgagcgccac tccggtcgtg
    25681 taactggcgg ccggcgagac gaggtacagc accgcgccgg ccatctcgtc gggctcggcg
    25741 gcgcgattca tcgggatgtg cggcagcacc ttctcgagca ccgcgggatt gcgggtgagc
    25801 gtcgcagcga acttcgtgtc ggtgaggccc ggcagcagcg cgttgacgcg gatgccctcg
    25861 ggtgcgcact cgcgcgcgaa cgccttggtc atcgaaatca cggcggcctt ggtgatcgag
    25921 tacacgccct gcagcggtcc cgggatcacg ccgttgatcg aggcgacatt gacgatgctg
    25981 ccgccgccgc cccgcgccat cagcttcgcg ccggcctgcg acatgaagaa atagccgcgg
    26041 atgttcacat cgaccgtctt ctggaacgcc gccggatcga tcgaggtgat cgggccgtaa
    26101 tgcgggttgg tcgcggcgtt gttgacgaga atgtcgagcc gcccgtgcgc ggactcgatc
    26161 gcggcaaagg ccttcgcgat ctcgtcgagt tcgccgatgt ggcaggcgag ggcgctcgcc
    26221 tcaccgcccg cggcgcggat cgaggcggcg accgcctcgc aggcctcgag cttgcggctc
    26281 gagacgatca cgtgagcccc ggcccccgcg agcgcgcgcg caatcgactc gccgatgccg
    26341 cgcgatgcgc cggtgacgag ggccacacgg cccgacaggt cgaacgaggg ggtgctggcc
    26401 ataagactcg ctagcctagg cccgggtatg gaatcattca aatgaatgtt ccgaatcccc
    26461 gggaataagt actgtgcata tacgtggcat cgacctcaat ctcctcgtgg tgctcgacgc
    26521 gatcatcacc gagggcggca tcaccagggc ggccgacaag ctgaacctga cccagcctgc
    26581 gatcagccat gccctcgggc gcctgcgcat cctgttcgac gatccgctgt tcgtgcgcga
    26641 gggtcgggcg atgcgcgcga cgccgaaggc gcgcagtctc gccgagcccg tgcgccgcgc
    26701 gttgcgcggc ctcgaggtca tgctcacgga aaccgaatcg ttcgatccgg cgacgagcca
    26761 gcggcgcttc acgatcggcg tgcgcgacgt gttcgaggca accttgctgc caccgctgat
    26821 gcgggcgatc cagcgactcg cgccacacgt ccaggtggcg gcgctgatgg tgccgcgggg
    26881 cgagctcgaa tccgaactcg ccgcgggccg cctcgacgcg gcactcgatg tgcgctggcc
    26941 gatgagcgag gcgatccgcc gccagcgcat cgccgaagag cgcctggtgg tggtcgtgcg
    27001 ccagcgccat ccgattgtga ggcgcggcct cgacctcgac cagtacctgg cgcaggagca
    27061 catcgtggtg agctcgcggc gcagcggcga gggcccgttg gaccaggaac tcgcgcgcct
    27121 cggcctgcgc cggcgcatgc gcctgcgctg ccagcattac ttcgcggcct gccgggtggc
    27181 cgcgatgacc gatctgctgc tcacgatgcc cgagcagtac gcgcgcatcg tgaacaagaa
    27241 gctgccgaac caggtcctgc cgctgccgtt cgacatggcg cccctcgatg cctacctcta
    27301 ctggcacgcc gaggcggacc gcgaccccgc caatcgctgg ttgcgtgagc aactggcgcg
    27361 ggtgttcgcg ccgcgtgcgc gctagcgcgg cgcgtccttc ctcttccccg gcggcaagcc
    27421 actcgcccgc gtcagcacgc tgctgccgaa gcgctcccgt atggcatcca gcgcggaatc
    27481 gagcggtggc ggctgcggaa cgctgcctgc cgcgaacaga tcgagctgcg tcgccggcgc
    27541 cagcccggtg acgccgacgc cgagcaggcg cacgcgcgcg cgcggatgct ccgcgcgcca
    27601 cttgcgcagc aggtcacagg ccgcgcggcc gatcacgcgg ctgtcctgcg tgggcggctc
    27661 gagctgctgc tggcgcgtga acgtcgcgaa atccgcgcgg cgcactttca ccgccacaca
    27721 gccggcggcg agttccttcc tgcgcagccg cgcgctcgcg cgatcggcga gtgcaagcag
    27781 ctccgcctcg aggcgcgccg gatccgcgag gtcggtgtcg aaggtttcct cggcgctgat
    27841 cgacagatcc tcgcgatcgg gcacgaccgg gcgatcgtcg atgccgcctg cacgctcgcg
    27901 catgcggcgc gtgtcgcggc cgaacagggg ccacagcacg gcatcgggtg cgcgccgcag
    27961 ctcccccagg gtcccgatcc cggcgcgctc cacctcgtca cccttcttgc gaccgagtcc
    28021 gggcaggcga cggatgggga gcgcatccag ccgccccgga agctccgcga cggtgatcac
//
LOCUS       DAOQVY010000020        27855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_2011_length_27855_cov_5.126259, whole genome shotgun sequence.
ACCESSION   DAOQVY010000020 DAOQVY010000000
VERSION     DAOQVY010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 27855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27855
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2011_length_27855_cov_5.126259"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /gene="mltG"
                     /locus_tag="PKN91_03360"
     CDS             complement(<1..947)
                     /gene="mltG"
                     /locus_tag="PKN91_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNS27142.1"
                     /translation="MRSWRAALVWLLALVVLGAIAGYAWVNRLMHEPGPHAEVVRVHV
                     YPGRSLRATLQQIEGQGALRHAQVIEWYLRLRREPLAPKVGNYDLPARASPREIVDLL
                     HSGRVVLEQLTIVEGWTFADMRRTIEAHPRIRHTLKGEPAEAIMAAIGHPGQHPEGRF
                     FPDTYRFADGTLDREIYRLAYDTLARTLAAAWDARHADLPIHTADEALILASIVEKET
                     ALASERARIAGVFTARLRSGMRLQTDPTVIYGLGEKFDGDIRRRDLTADTPYNTYTRA
                     GLPPTPIALPGREAILAAVQPDETGELYFVATGLGDGSHV"
     gene            complement(940..1776)
                     /gene="pabC"
                     /locus_tag="PKN91_03365"
     CDS             complement(940..1776)
                     /gene="pabC"
                     /locus_tag="PKN91_03365"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR03461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HNS27143.1"
                     /translation="MLGAWVDGSVAATIGIDDRGLHYGDGLFETIAASAGRPRFLAGH
                     LARLARGCAALSIPMPDEARLRAEVAQAAALAPHVIVKLIVTRGAARARGYAIEGGEA
                     PRRIVIAYAWLEGPDHAAAPARVARAERPVARPPAALAGVKHLNRLEQVLALTEARLR
                     ALDEVLLCTDDDRIVGGSMTNLFLVTGGELVTPPAASCLVAGVMRGEVLRAAARLGLA
                     VAERAISVADLSRVEGAWLTNARVGLWPIGAIADRSLAVHAWHSPLQRQVRQAALGVQ
                     DA"
     gene            complement(1776..3155)
                     /locus_tag="PKN91_03370"
     CDS             complement(1776..3155)
                     /locus_tag="PKN91_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNS27144.1"
                     /translation="MSLRETRRRLDIPSATLRRLVGEHPDLFPVLFDSAAAGSRSMLA
                     AMPGGLLGVDREGRLVVEGDVPAAVAAQGRAGGFLAALDAWWRSVHSEAPHGGGLAGG
                     WVVYLGYEMAQEIEPRLRLPRGSLPWRALALRVRAALEHDLAGDVIEAVAAPGSEALL
                     PAIEQLARAAAAEQPASPGFPRGAAQIRIEEEPPERFLERVAQAQEHIRAGDIYQANL
                     SRWWRAPWPEGDAYALYERLCAANPAPFAAFARLRGMDILSSSPERLLQVAAREASTR
                     PIAGTRPRSRVPGEDSTEAAALTAHPKERAEHVMLIDLERNDLGRICKPGSVRVDEFM
                     VVESYAHVHHIVSNVRGTLRDEVTPVDALRAVFPGGTITGVPKVRCMEIIAALEGAGR
                     DAYTGSLGWIATNGDADFNILIRTLTLADGRIEARAGAGIVADSEAGRELEETRAKAR
                     GLLAALEEP"
     gene            complement(3171..4418)
                     /gene="fabF"
                     /locus_tag="PKN91_03375"
     CDS             complement(3171..4418)
                     /gene="fabF"
                     /locus_tag="PKN91_03375"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNS27145.1"
                     /translation="MGNRRVVVTGIGIVSPVGSTLDSAWAAVLAGQSGIAPITRFDVS
                     AFGTRFAGEVRGFDVTAYLAPKEARRMDPFMHYGVAAGTQAVTDSGLDFSKEDPTRSG
                     VIMGSGIGGLEGMEREIGTWATTKNPRKISPFYIPSTIINMIAGHLSIRYGINGPNLA
                     VVSACTSSAHALGLAMRMIQYGDADIMIAGGAEHASTPTALAGFGQAKALSERNDDPA
                     GASRPWDRDRDGFVMGDGGGAVILEELGHAKARGARIYAEMIGFGMSGDAFHITAPPD
                     DGRGARIAMVNALRDAKLAPEDVQYLNAHATSTPRGDTAETIAIKHAFGSHAARLAVS
                     STKSMTGHLLGAAGVAESIFSVLAIRDNVAPPTINYHTPDPDCDLDYVPNEARPMPIE
                     VAASNSFGFGGTNGTVVFRRFKG"
     gene            complement(4485..4724)
                     /gene="acpP"
                     /locus_tag="PKN91_03380"
     CDS             complement(4485..4724)
                     /gene="acpP"
                     /locus_tag="PKN91_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNS27146.1"
                     /translation="MSTVEQQVKAIVTEQLGVKAEQVTSDASFVDDLGADSLDTVELV
                     MALEEEFEIEIPDEDAEKITTVQQAIDYINERRKS"
     gene            complement(4894..5634)
                     /gene="fabG"
                     /locus_tag="PKN91_03385"
     CDS             complement(4894..5634)
                     /gene="fabG"
                     /locus_tag="PKN91_03385"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNS27147.1"
                     /translation="MLEKDTALVTGASRGIGRAIATALLAAGANVIGTSTSAEGAAKL
                     TADFAAAGQRGRGAVLDVGDAASIDTLVADLDSKGEMPTILVNNAAITRDTLLLRMKQ
                     DDWDSVLATNLTSVFRLSKACLRRMMKERRGRIVNLTSIVALTGNPGQANYAAAKAGL
                     LGFTKSLAKEVASRGITVNAVAPGFIDTDMTRSLSDEQRGALLAQIPMARLGAADDVA
                     AAVAFLCSPAAAYITGETLHVNGGMYMP"
     gene            complement(5637..6581)
                     /gene="fabD"
                     /locus_tag="PKN91_03390"
     CDS             complement(5637..6581)
                     /gene="fabD"
                     /locus_tag="PKN91_03390"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNS27148.1"
                     /translation="MGIAFVFPGQGSQSVGMLGALAEAEPIVRETFAAASSVLGYDLW
                     SLIAAGPAERLNETERTQPAMLAAGIATFRAWQTRGGALPNAVSGHSLGEFTALVAAG
                     ALDFGAAIELVQFRGRAMQEAVPLGSGAMAAVLGLEDADIEAACREAAQGAVVEPVNY
                     NSPGQIVIAGESAAVARAIEAAKVRGAKRAVLLPVSVPSHSSLMRGAAERLRERLGGV
                     AIAVPRIPYYSAVDSKRHDDPDGIRAILVTQLASPVHWTATVRALIADGMTTLAECGP
                     GKVLTSLNRRIERAPGLTFHALEDPVSIDAARAAMQGE"
     gene            complement(6610..7212)
                     /gene="rrtA"
                     /locus_tag="PKN91_03395"
     CDS             complement(6610..7212)
                     /gene="rrtA"
                     /locus_tag="PKN91_03395"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HNS27149.1"
                     /translation="MTAVPAPSRLLASLNGDGRYGLGLACVLALLLLPSLAGEVGRLA
                     WRYERTGVAAGEFWRLASAHWVHLDLRHALLNGAGCALVWALFARDYRPVHWLAIVGV
                     SMLAIDAGLWFGAPGIEWYVGASGWLHGVMAAGALAQLRRREHVGWIVALVLAGKLLA
                     EQYMPLPFAGDAPVVVQAHLYGAVGGLVIALCLPSRREPL"
     gene            7250..7687
                     /gene="gspG"
                     /locus_tag="PKN91_03400"
     CDS             7250..7687
                     /gene="gspG"
                     /locus_tag="PKN91_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNS27150.1"
                     /translation="MNQPARSRGFTLIEIMVVVVIIGLLAAFIAPQILGRVDDAKIVK
                     AKQDIQALETALTMYKLDTFRYPGTDLGLVALVQKPADPQVRNWREGGYLKRVNKDPW
                     GNDYQYVFPGTRGGEYDLYSLGADGEPGGEGINADIGNWNLDR"
     gene            7712..8233
                     /gene="gspH"
                     /locus_tag="PKN91_03405"
     CDS             7712..8233
                     /gene="gspH"
                     /locus_tag="PKN91_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HNS27151.1"
                     /translation="MKHARGFTLIEILVVVVIIGVLAVGAVLAIGTSKRDDTLEKEAQ
                     RLEALIAYAREHAELATREFGLRIEADRYGFLVYDPRRGIWHSADADEVLRERALPVG
                     LRVRLRIEGRDVLLRPLKTGEPLMPQLMLFSNGDVTPFELMLDRAGSELRATLASDEA
                     GAIKLTLPQDARP"
     gene            8233..8766
                     /gene="gspI"
                     /locus_tag="PKN91_03410"
     CDS             8233..8766
                     /gene="gspI"
                     /locus_tag="PKN91_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HNS27152.1"
                     /translation="MRPRGFTLIEVLVALVIVGVGMGALLATLTSAADSSIYLRDKTF
                     AEWIALNRISELRLAAKAPSKGKSSGQINFVGRQWNYEQEVVETDIPGLLRIDVSVAD
                     TATQAAGNTKRKSWTVTASGVVGDALLAPNGQEPDWNGGPFPGEPVRGRQDGQEPPPD
                     DESTPVPPPSGSPLPTS"
     gene            8766..9380
                     /gene="gspJ"
                     /locus_tag="PKN91_03415"
     CDS             8766..9380
                     /gene="gspJ"
                     /locus_tag="PKN91_03415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HNS27153.1"
                     /translation="MRPRGFTLVELLVALFISAIMFAMGYGALQQALSNREAVQANQD
                     RLSALVNTMRIFAQDFGQLAPRPTRSLVGDGADPALQADGRSEALATFTRFGWANPAG
                     IQRPALQRVRYVIEDGTLYREHWHVLDPVLGAQPARRALIDGVRTVRFRFMESGRSWT
                     EQWPGIVTAGAPRQPQLRRRPIAVEVVLDVEDFGTITRIFEVPG"
     gene            9377..10309
                     /gene="gspK"
                     /locus_tag="PKN91_03420"
     CDS             9377..10309
                     /gene="gspK"
                     /locus_tag="PKN91_03420"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HNS27154.1"
                     /translation="MKRERGVAMLTAIILVALATIVAASIAFVSGMTARRTSGAFMFD
                     QGIQFAGAAEAMAAYVLREDRKDNQTDDYTETWAQPYGPVELIEGSGIWLEARLDDAQ
                     GRFNVNNLVDTEGVVRGEAVEQFKRLLAMLQLEEKWATLLADWIDADDQPNGFEGGED
                     TLYLAQSPAYRAPNAPLVSTSELLALPGFGNERYLRIAPYISALPPGTPLNVCTAPGI
                     VIDAMTEEREYGLDQERLAKSRESGCFPTKADLQAKIDAEKWITLQYQISDSSEYFRL
                     RSFVGIGTMELALYSLLKRESTGVRPILRTYGTD"
     gene            10313..11566
                     /gene="gspL"
                     /locus_tag="PKN91_03425"
     CDS             10313..11566
                     /gene="gspL"
                     /locus_tag="PKN91_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HNS27155.1"
                     /translation="MADWFVVRLPRASGDPASWIAVDEQGHMLAAPGAGTLAQAAAQA
                     AGRRVAALVPADHVLLTDAALPAKAGAKLAQLVPYALEEQVADDIDALHFAVGRRNPE
                     TGRTSVGVVDRSIMQSAGEALLKAGIAAVALYSEASLIAPNPGQVVAWLDGDTLVVAS
                     PARAPIVMPVTSLREVFEFLASTHAYGEGVLPATLSLQLVATSLDWQQHAGELDFLQE
                     HFAGARVQLLPQGSLPWLASQIRAAAPLNLLQGDYAARIAGGAELRRWRVPAALAAAL
                     LLVFVAGKVYQVQRLDAAERALDAEIDQTFRMAMPGAADSTDARRRMAQRLASMQDGV
                     GSAGLLPALSAFASARAAVPDASLHGISLRDGSLDLRVRAADAASLDQINQQLRASGY
                     TTELSSGTAEESGYEGRIHIRLAGA"
     gene            11563..12048
                     /locus_tag="PKN91_03430"
     CDS             11563..12048
                     /locus_tag="PKN91_03430"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein M"
                     /protein_id="HNS27156.1"
                     /translation="MMEAARQWFNGHAERERRFVIYGGVAAIVIVLLMAVLPLQRSAA
                     ALEQRVATKRADLAWMRSVAPALAGAGPVSDTPLTQESLVVTVDRTARESSLGEALAR
                     SEPAGQGGQRVQLNGARFDVMVAWLARLRQQHGIGVVAATIERSGEPGLVNATVELNA
                     L"
     gene            12045..12770
                     /locus_tag="PKN91_03435"
     CDS             12045..12770
                     /locus_tag="PKN91_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HNS27157.1"
                     /translation="MKPLRLAALVAAALLVTLLLRWPLSWTVHWLPDSLHCAYPAGSV
                     WRGRCAALTVEQLRLGETSWQLRPLGLLRGALTASVQSRQGPDRLMADIELRPGGRRI
                     ARNVEAELRLGEGLLQRSAMGLTGQLQLQLSRVVLDERTVREIEGTVTARKLVQGASR
                     ATPLGDYELVFPPGSSQASLRDLGGPLGVTGTLTLTGEPGYVVEGFVTVRPAAPQPLA
                     RTIAMLGTPDSAGRRPFSFAGTY"
     gene            complement(12764..16048)
                     /locus_tag="PKN91_03440"
     CDS             complement(12764..16048)
                     /locus_tag="PKN91_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNS27158.1"
                     /translation="MTEPASRLADLDTFAREHAIRIDTSVLLEAPAGSGKTTVLTQRL
                     LALLAVVDEPEQVLAITFTRKAAAEMRARVLAALAGDIDPAHPQAPLLRRLADGVHAR
                     SRERGWQLEAQASRLRILTIDAFNYSLAAQLPLVAGSGTTPSIAERPRDLYQRAARRA
                     LLEGEAEPDMRRDLDLLFDRLDNQWRKVESLLAGMLARRAHWLPHVLTSDTGDLRARI
                     DASLTRIVAEQLARNVARLPRGLRAAGSALPDVGALDASPAAFAAWDRFAATVLTQAG
                     TLRKRVDARIHPAYRDAATKSALKSVLDELAGIDGAEQLLRETRELPPPGLEEADGEA
                     IDALARVLTWVARYLQLEMAQAGRVDHVHVAGAARAALTAEGGASDLAIRTGSTLRHI
                     LVDEFQDTSIAQFELLEMLVQDWSAGDGRTLFVVGDPMQSIYQFREAEVGLFLRARDH
                     GIGPVKLEALRLTRNFRSRPALVDFNNELFARLFPDHDDLRAGAVRYSPSAAAVAETP
                     GSAEAVSLLALANGDGDDDARAVLGRVRALREREPQASIAILVLARAHAVPISARLVA
                     AGYAVRGVDLVPLAEVPAVRDLVALLRALLHPGDRTAWLTLLRAPWCGLKLTSLRVLS
                     GRKDPLLVWEAMQSAERTAQLAPDEASRLARVREVVGRAFAGPGQGAPGEALESVWMQ
                     LGGPECYPVAELDAAREFFRALDAAWSRGEWQGSASIDTMLADLYARPDSAAPGAIEI
                     MTIHRAKGLEFDHVILPGLGRRPRSDEEPLLRWLDLPRTDRDSDLLMSPIVAAGERDE
                     HRLGAYIRQLGRARRAHEQRRLLYVAATRARRSLHWIAGVAVKDDDDPRPAGGTMLAA
                     AWPVPAGRFEVVDTGPANAAIDASVATRLERLPGDWQAAALADAVTTESLFLAREPLD
                     TPEFSWVRATARHIGTAVHAALESWGRALPASAAGIEAGAAAQRTLLARLGVPESELD
                     AATATVMQALRRTWADPRGQWLFDSRHRHGATELALTGLVDGRIVNVLFDRTFVDADG
                     VRWVIDYKTSRHEGGDLETFLASEMERYRPQLARYAVLAGALGPEPVRTALYFPLLGI
                     FRAYDQ"
     gene            complement(16041..18698)
                     /locus_tag="PKN91_03445"
     CDS             complement(16041..18698)
                     /locus_tag="PKN91_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNS27159.1"
                     /translation="MITVTDEISTAIERGDTVVVPSPQRAHALRVAHARRALARGEVV
                     WPTSDVLSGEGWLAREVTRAANGDSSLPRPLAAAEEWWLWREATARAAQDTALGGTAA
                     LADSLRRADRLAAEHGIDIARWRIIGGLETQLLDTVRRAVSQARRRLGADAVANIAAL
                     MDGLADARSVHFAGFAALEVPRLRAIHAARREKGQGGEWWGVGEPRAKPVVVLAGDEA
                     DEIGRISAWCLRRLERDADARVLVVAATRLEQREALAAQIRATLAPASKFSGDSNDGL
                     VAVEGGVPLTRQAIVQQSLAGLAWLIDGLEFDAFTTWLRSPCGVLAAEAAARLDIWWR
                     RHAPIEADARASLALLDRAAAGGLAGAATLAERVRAALAVLAAGAADARGWAERFIGA
                     LAALRAAAEPAGLDSAGQQTWLRFVALLDEFGAVAAVAGRLDAHAALAALRELAARTT
                     YQPATGDALVTIAATHDDPVAQYDGIWIASLTADAWPAPAMTDPFVPLPALREAGVRA
                     ASGAGQLAAARQSLAAWRAATEHLVLSATTVSGDMHVSPSPLLAAWPRSEAPEDRTAS
                     LWLPLRIRRPLTLEVLADGTGTPWPAAQPLPGGTSSLQHQADCPFRAYARQQLGAEPL
                     DELRLGVEPDERGRWLHAALEAFWREVGDSTQLAALPPAQRRALARRVVDETREPLPL
                     CGGEAAVAAREREARRLVALIVDFAVLEDSRPAFRVAEMEQEKLIALGEARLAVRIDR
                     VDELSDGGRAVIDYKSGKPQRFDWYGERPQAVQLLTYLSATGEAVRALANAHVTRHGM
                     CYVGITAPDVSLPKVPVLQVNPGETDDAAWARQVGAWLAGLRGLAAEFLRGEAAVTPA
                     KSACRYCHLGSLCRVGERARFEDGEAAGD"
     gene            complement(18728..20002)
                     /locus_tag="PKN91_03450"
     CDS             complement(18728..20002)
                     /locus_tag="PKN91_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNS27160.1"
                     /translation="MTETFRPSDSLRNVRYEIRGRLARRAHEMERLGHEIISLNIGNP
                     GLFGFRAPETMRLAMIENLGASEAYCHQKGIFPAREAVVMQQQARGVQGVSAEEVFIG
                     NGVSELIDLVLRAMLNDGDEVLVPSPDYPLWTAAVNLNRGRAVHYPCRPEDGFVPDPD
                     AVEALITRRTRAIVIINPNNPTGAVYPRAVLERLARIAEKHKLVVLADEIYDQIVYDG
                     ATFVPMAMLVHDTLCATMSGLSKVYRACGYRVGWTVFSGRLRSAGDYLNALELLSSLR
                     LCSNVPAQWAVQTALGGYQSIKDLTAPGGRLHDSRAVILDAAAASPYLQLQAPLGALY
                     AFVGVDSNAMPGFDDQAFALDLLEQKHVLVAPGVSFNVPYRNHFRITNLPDAATLKTV
                     FQRMNELLATYAAGGPSGAGTGQVVDAAARFK"
     gene            complement(20030..21253)
                     /locus_tag="PKN91_03455"
     CDS             complement(20030..21253)
                     /locus_tag="PKN91_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNS27161.1"
                     /translation="MGPLHGIRVLDLSRVLAGPWATQALADLGADVIKIERPDGGDDT
                     RQWGPPYARDAEGRDTTESAYYLSTNRGKRSVAVDLSNPQGASLVRALARRCDVLVEN
                     FKVGGLAKYGLAYADLKAECPGLVYCSISAFGQDGPDADKPGYDAMIQGMGGLMSITG
                     LPDGEPGGGPQKVGVAVVDLMTGMYATTAIIAALYERRASGIGQYIDLALLDTQLGWL
                     ANQNMNFLVTGESPRRQGTAHPNIVPYQAFATSDGFLMLAVGNDRQFSAWCSVAQQPQ
                     LAADVRFATNKARVAHRAELVPIVAAALRAHPTRWWLDALNAANVPCGPINEIGQVFA
                     EPQIVHRGMRIDLPHPTAGTVPGVKNPINYSRTPLEYRSAPPLLGEHTDTVLEAELGL
                     DSTELARLRAAGAIR"
     gene            complement(21287..22243)
                     /locus_tag="PKN91_03460"
     CDS             complement(21287..22243)
                     /locus_tag="PKN91_03460"
                     /inference="COORDINATES: protein motif:HMM:NF025852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4437 domain-containing protein"
                     /protein_id="HNS27162.1"
                     /translation="MARPHIEPFVDRDVQFRKFTMPGFPKGMHYKMLSLDPDNGACTM
                     TVMFEPGYKQPPGMSYSEYELFIMTGSITVGDKVHGPGSYFMVPAGVALGALSSSRGA
                     TGLLYYNYGEPSFVESDSDHPRAERSKFVAVNAYDDMQWTSTNVFPATAPGCLVKVLH
                     FDERTQAFTFLYCMTPNFWQDNISYHDCAEEAYHIWGTSWMMQFGDLPTGGYFYRPPY
                     INHGAFGCKLGTLALGRTDSRLYNHFHFNPWTNVEENRERAASRIQHWMPDLYKWINT
                     HGHNHPVDFDFPHGHEHGDMPHHHGDGQVEHAHKSRRRRRKK"
     gene            complement(22269..22523)
                     /locus_tag="PKN91_03465"
     CDS             complement(22269..22523)
                     /locus_tag="PKN91_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27163.1"
                     /translation="MKSIPHKRSREVEAWLKREIAEQKVRYNSIVAEQDALAPKRDKW
                     VAEFLERIQTRGVHVHFNMMRKVSPEEIPVRPKRKFRVVF"
     gene            22649..23560
                     /locus_tag="PKN91_03470"
     CDS             22649..23560
                     /locus_tag="PKN91_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27164.1"
                     /translation="MLDAIIAVTLSAPDLGAAERAYHAQLGYQVVQRAQVSRSLAASW
                     DARAMRGRDYVLMQPASGEPVYLRIVSAEAPLPGYAPLRRYGWNANEILVEDPVALAS
                     RLREPFKEIGKPRPLDFNPSIIAMQVTGPAGEVNYFTRIPPTGSVFKLGSAKSFVDRT
                     FIVVVGGPDVGALRAFYRDVLQAPTTDPQPTKINVLQNAYGLPPDAETLMGIVQFPSS
                     FLIELDQYPEGAVDRPIRRGQLPPGIAMVTFAVKSFDTMRLPWKVKPAVRSEAPYNGR
                     RAGLVIGPAGEWIELVETGGSNPGSGP"
     gene            complement(23563..24378)
                     /locus_tag="PKN91_03475"
     CDS             complement(23563..24378)
                     /locus_tag="PKN91_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HNS27165.1"
                     /translation="MSASTGAPLLRVSQVGVRYGRGRGVGRAAGFVALHDISFDVEAG
                     DSIGIVGESGSGKSTLLRALLGLMPIADGSIAWHGRDLASFDAASWLALRRQVQPVFQ
                     DPLASLDPRMRVRALLAEPLQVHRPELARAAVDEAIAKMLDLVGMPAGVLASYPHELS
                     GGQCQRICIARAMMLGPAMLACDEPVSALDVSIQAQIMQLLQSLHRDSNLTLVFVSHN
                     LALVRRLCRRIIVLRQGRIVEAGDAVQVIDRPQHAYTQALVAAVPVVARRRLG"
     gene            complement(24375..25340)
                     /locus_tag="PKN91_03480"
     CDS             complement(24375..25340)
                     /locus_tag="PKN91_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS27166.1"
                     /translation="MSSIPAPALELRDLAVRFAARGGPLVAVAGVSLRIAPGECVGII
                     GESGAGKSQALLAPFRLTASDAQVGGMARLAGEDLLAKSEAELDRVRGARAGFVFQDP
                     MSSLTPHRTVGEQIIEVLECHGRAHGAAARTRTLELLEQVRIDDAARRLSRYPHELSG
                     GQRQRVAIAIAIACDPPLLIADEPTTALDVTVQAGILELLAALKRERHMAMALVSHDF
                     GVIARLADRILVMYAGRVVEEGTAAALVAAPRHPYTAALLECLPRLDDPPGAPWQAIP
                     GQPPDPRALPAGCAFHPRCPRAAERCRVRQPQLDGGVACHLPVSA"
     gene            complement(25312..26166)
                     /locus_tag="PKN91_03485"
     CDS             complement(25312..26166)
                     /locus_tag="PKN91_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS27167.1"
                     /translation="MPGSTRVSGGIELPQGRALRVAAALLLALCMLALFAPLLSPHAP
                     EAIDWSRLAAAPGAADAHWWGTDRLGRDVFARTMQGLRISMLIGFVAALVSVAIGLLW
                     GAIAGYAGGRTDAVMMRIVDVLYALPYVFLVIILATLFERGSIPVLLIAIASVGWLTT
                     ARIVRGQTLALRQREFVTAACAMGVPTARILWRHILPNVAGPVIAYATLTVPQMILYE
                     SFLSFLGLGVQEPLASLGSLVSDGARDMETAPWLLLAPAGCLVVLTLLLNILGDGLRD
                     VLDPRTRA"
     gene            complement(26132..27055)
                     /locus_tag="PKN91_03490"
     CDS             complement(26132..27055)
                     /locus_tag="PKN91_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNS27168.1"
                     /translation="MMRHAVLRIIGLVPTLLLLVTAAFFVIRLAPGGPFDDERPALPE
                     VRANLEAAYGLDAPLTTQYLRYVRGLLHGDLGPSFKHKDSSVSELIGAGLPISATLGA
                     AALALALLLGIPLGVGAALRRGSLTDRAVALLAALAIALPTFVLGPLLALLFGVRLRW
                     LPAAGWDAGTLRHAILPVVTLAIPVLAYVIRLTRTGLIEVLGLDYIRAARARGIGMRR
                     LVVTHALRPALLPLVSWLGPATAFVLTGSLIVETVFSIPGAGRFLVEGALNRDYTLVM
                     GMTLVYGTITLICNLLADLLYAWLDPRIRRH"
     gene            complement(27066..>27855)
                     /locus_tag="PKN91_03495"
     CDS             complement(27066..>27855)
                     /locus_tag="PKN91_03495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS27169.1"
                     /translation="LPSVFTQLIETYEWRDAASMSALAPLALLPIIWFSIREWPARIG
                     LAPYGASSPAGSEQLAGPLLSYRDIVARRQFWLIGLGAFATFYSILGISNNLFLHSRD
                     LGFAPSESAALFVPLFILGLIGKVVSGVLSDILGRKRVWILSLLLMLGGALMLMTLQK
                     SLVMFAIMLFGFGWGGNYSLLQAIAADAFGSRSLGRVMGAITVLDAGGGALGPWITSL
                     LYDAYGSYTPGFGLICVLIAVAIAFAMVLKVRAPVVSPAAVSAA"
BASE COUNT         4505 a         9739 c         9457 g         4154 t
ORIGIN      
        1 acgtgcgatc cgtcgccgag gcccgtagct acgaaataga gctcgccggt ttcatcgggc
       61 tggacggcgg cgaggatcgc ctcgcgaccc ggcagcgcga tgggcgtcgg cggcaggccc
      121 gcgcgcgtgt aggtgttgta gggcgtatcg gccgtaaggt cgcgccggcg gatgtcgccg
      181 tcgaacttct cgcccagccc gtagatcacc gtcgggtcgg tctgcagccg catgccgctt
      241 cgcaggcgag cggtgaacac cccggcgatg cgcgcccgtt cactggcgag cgccgtttcc
      301 ttctcgacga tcgaggcgag aatgagtgcc tcgtcggccg tgtggatcgg caggtccgcg
      361 tgccgcgcgt cccaggccgc cgccagcgtg cgtgcgagcg tgtcgtaggc gagccgatag
      421 atctcccgat cgagagtgcc gtcggcaaag cgataggtgt cgggaaagaa gcgtccctcg
      481 ggatgctggc cggggtggcc gatcgccgcc atgatcgctt cggccggctc gcccttcagc
      541 gtgtgcctga tgcgtggatg cgcctcgatc gtgcgtcgca tgtcggcaaa ggtccagccc
      601 tcgacgatcg tcagctgctc gagcacgacg cgtccggagt gcagcagatc gacgatctcg
      661 cgcggcgagg cgcgcgcggg caggtcgtaa ttgcccacct tcggggcgag cggctcgcgc
      721 cgcaggcgca ggtaccactc gatcacctgc gcgtgcctga gtgcgccctg cccttcgatc
      781 tgttgcagcg tcgcccgcag gctgcgcccc ggatagacgt gaacccgcac gacttcggca
      841 tgcggccccg gctcgtgcat cagccggttc acccacgcgt agccggcgat ggcgccgagg
      901 acgaccagcg cgagcagcca taccagggcc gcgcgccagc tacgcatcct gcacgcccag
      961 agccgcctgc cggacctgcc tctgcagcgg cgagtgccac gcatgcaccg caaggctccg
     1021 gtcggcgatg gcgccgatcg gccacaggcc gacgcgcgcg ttggtgagcc aggcgccctc
     1081 gacgcgcgac aggtcggcca cgctgatcgc gcgttcggcc acggccaggc ccaaccgtgc
     1141 ggccgcccgc agcacctcgc cgcgcatcac accggcgacc aggcaggacg ccgccggcgg
     1201 cgttacgagc tcaccgcccg tcacgaggaa cagattggtc atgctgccac ccacgatccg
     1261 gtcatcatcg gtgcaaagca ggacttcgtc gagtgcgcgc aggcgcgcct cggtcagcgc
     1321 gaggacctgc tcgagacggt tcaggtgctt gacgccggcc agcgccgccg gcggacgcgc
     1381 aacgggccgc tccgcgcgcg ccacgcgggc cggcgccgcg gcgtgatccg gcccctccag
     1441 ccacgcgtag gcgatgacga tgcgtcgcgg cgcctcgcca ccctcgatgg catagcctcg
     1501 cgcgcgcgcc gcaccgcgcg tcacgatcag cttgacgatc acatggggag cgagcgctgc
     1561 ggcctgcgcc acctcggcgc gcagccgcgc ctcatcgggc atcgggatcg acagcgccgc
     1621 acagccccgc gcgagtcgcg cgagatggcc cgcgaggaac cgcggccgcc cggcactcgc
     1681 cgcgatcgtc tcgaagagcc cgtcgccgta gtgcaggccc cgatcgtcga tgccgatcgt
     1741 cgccgcgaca ctgccatcga cccaggcacc gagcatcagg gctcctccag cgcggcgagc
     1801 aggccgcgcg ccttggcgcg tgtctcctcg agctcgcgcc ccgcttcgga atcggccacg
     1861 atgcccgcgc ccgctcgcgc ctcgatccgg ccatccgcga gcgtgagggt gcggatcagg
     1921 atgttgaagt ccgcgtcacc gttggtcgcg atccagccga gcgagccggt gtaggcatcg
     1981 cggccggcgc cctcgagcgc ggcgatgatt tccatgcagc gcaccttcgg cacacccgtg
     2041 atcgttccgc cggggaatac cgcgcgcaac gcatcgaccg gggtcacctc gtcgcgcagc
     2101 gtgccgcgga cattcgacac tatgtggtgc acgtgcgcgt aactctcgac gaccatgaat
     2161 tcgtcgacgc gtacgctgcc cggcttgcag atgcgcccaa ggtcgttgcg ctcgaggtcg
     2221 atcagcatga cgtgctcggc gcgctccttc gggtgcgcgg tgagcgccgc ggcctcggtg
     2281 ctgtcttctc ccggcacgcg gctgcgcgga cgcgtgcccg cgatcgggcg cgtgctggct
     2341 tcccgcgcgg cgacctggag caggcgctcg ggggaagagc tcaggatgtc catgccccgc
     2401 agtcgcgcga aggcggcgaa cggcgcggga ttggcggcgc acaggcgttc gtagagtgcg
     2461 taggcgtcgc cctcgggcca gggcgcacgc caccaccgcg acaggttcgc ctggtagatg
     2521 tcgccggcgc gaatatgctc ctgggcttgc gcgacacgct cgagaaagcg ctccggcggc
     2581 tcctcttcga tccggatctg tgccgccccg cgcgggaatc ccggtgaggc aggctgctct
     2641 gccgccgcgg cacgcgccag ttgctcgatc gcgggcagca gtgcctcgct gccgggtgcc
     2701 gcgaccgcct cgatcacatc gcccgccagg tcgtgctcga gcgccgcccg caccctcagg
     2761 gcaagggcgc gccagggcag cgagccgcgc gggagccgca gccgcggctc gatctcctgc
     2821 gccatctcgt agccgaggta aacgacccag ccgccggcca gaccgccgcc gtgcggcgcc
     2881 tcgctgtgca cgctgcgcca ccaggcatcg agtgctgcca gaaagccgcc cgcacgaccc
     2941 tgcgcggcga ctgccgccgg cacgtcacct tcgacgacca gtcggccctc acgatcgaca
     3001 cccagcagtc cgcccggcat cgcggcgagc atcgagcgcg agcccgcggc ggcgctgtcg
     3061 aacaatacgg gaaagaggtc cggatgctcg ccgacgaggc gccgcaaggt ggcggaaggg
     3121 atgtcgagcc gccggcgcgt ctcgcgaagt gacatggcct gcggccgtac tcagcccttg
     3181 aagcggcgaa agacgaccgt tccgttcgtg cccccgaacc cgaacgaatt cgacgccgcc
     3241 acctcgatcg gcatgggacg cgcctcgttg ggtacgtagt cgaggtcgca gtccgggtcc
     3301 ggcgtgtggt agttgatcgt cggcggcgcg acattgtcgc ggatcgcgag taccgagaag
     3361 atgctctcgg cgacacccgc cgcgccgagc agatgcccgg tcatcgactt ggtcgagctc
     3421 accgccagcc tcgccgcgtg actgccgaac gcgtgcttga tcgcgatcgt ctcggcggta
     3481 tcgccgcgcg gcgtcgacgt cgcatgggca ttcaggtatt gcacgtcctc gggcgcaagc
     3541 ttcgcgtcgc gcaatgcgtt gaccatcgca atacgcgcgc cgcggccgtc gtcgggcggg
     3601 gcggtgatgt gaaacgcatc gccgctcatg ccgaagccga tcatctcggc atagatgcgc
     3661 gcaccgcgcg ccttcgcgtg cccgagttct tcgaggatta cggcaccgcc cccgtcaccc
     3721 atgacgaacc cgtcgcgatc gcgatcccac gggcggctcg ctccggcggg atcgtcgttg
     3781 cgctccgaca gtgccttcgc ctggccgaaa ccggccagcg cggtcggtgt gctcgcgtgt
     3841 tcggcaccgc cggcgatcat gatgtcagcg tcgccgtact ggatcatgcg catcgcgagc
     3901 ccgagcgcat gcgccgaact cgtgcaggcg gacactaccg ccagattcgg gccgttgatg
     3961 ccatagcgga tcgacaggtg cccggcgatc atgttgatga tcgtgctcgg gatgtagaac
     4021 ggcgagatct tgcgcgggtt cttggtcgtc gcccaggtgc cgatctcgcg ctccatgccc
     4081 tcgaggccac cgatacccga acccatgatc acgccgctgc gcgtcgggtc ttccttcgag
     4141 aagtcgaggc ccgagtcggt gacggcctgt gtgccggccg ccacgccata gtgcatgaac
     4201 gggtccatgc ggcgcgcttc cttgggcgcc aggtaggcgg tcacgtcgaa gccacgcact
     4261 tcgcccgcga agcgcgtgcc gaaagcggag acatcgaagc gtgtgatcgg agctatgccg
     4321 ctctgacccg ccagcaccgc ggcccaggcg ctgtcgagag tgctcccgac aggagaaacg
     4381 attccgattc cggtaacaac gacgcggcga ttacccacgc ggacctttcg caactcgtac
     4441 agcgggagga gcggcaaggc gccggcgggc cggcgccacg gacattacga tttgcggcgc
     4501 tcgttgatgt agtcgatggc ctgctgaacc gtagtgatct tctcggcatc ctcgtccgga
     4561 atctcgatct cgaattcctc ttcgagcgcc atgaccagtt ccaccgtatc gagcgagtcg
     4621 gcaccgagat cgtcgacgaa cgaggcgtcg ctcgtgacct gctctgcctt gacgcccagc
     4681 tgctccgtga cgatggcttt tacttgctgt tcaacagtac tcatctcagt gatgtcctcg
     4741 tgacttatct ttggtatggc cgcgggtagc cggccgaatg ggcgcggtat tgtaggtgaa
     4801 gccccttgcg ctcgccaccc gggctgcctg cgagtacttg ttttttcaga caaaacatgc
     4861 tcttgccgcg gcagcgtgcg tggcgccggc gcgtcagggc atgtacatgc cgccgttcac
     4921 atgcagggtt tcgccggtga tataggccgc cgcgggcgaa cacaggaagg caactgcggc
     4981 ggcaacgtcg tccgccgccc cgagccgtgc catcggaatc tgggcgagca acgcgccgcg
     5041 ctgctcgtcg ctgaggctgc gggtcatgtc ggtgtcgatg aagccggggg ccaccgcgtt
     5101 gaccgtgatg ccgcggctcg ccacctcttt cgccagcgac ttggtaaagc cgagcagccc
     5161 tgccttggca gccgcgtaat tcgcctgccc cgggttgccg gtcagcgcga cgatcgaggt
     5221 caggttgacg atgcgtccgc ggcgctcctt catcatgcgc ctgaggcagg ccttgctcag
     5281 gcggaacacg gacgtgagat tggttgcgag cacgctgtcc cagtcgtcct gcttcatgcg
     5341 cagcagcagc gtgtcgcgcg tgatcgccgc gttgttcacg aggatcgtcg gcatctcgcc
     5401 cttcgaatcg agatccgcga cgagcgtgtc gatcgatgcg gcgtcaccca cgtcgaggac
     5461 ggcgccgcgg ccgcgctgcc cggcggccgc gaaatccgcg gtgagcttgg ccgcgccctc
     5521 cgccgaggtc gatgtgccga tgacgttcgc gcccgcggca aggagtgccg tggcgatggc
     5581 acggcctatg ccgcgggacg cgccggtgac gagcgcggtg tctttctcga gcatgctcac
     5641 tccccttgca tcgcggcacg tgccgcatcg atcgacaccg gatcctcgag cgcgtggaat
     5701 gtgaggccgg gcgcgcgttc gatacggcga ttgagactcg tcagcacctt gcccgggccg
     5761 cattcggcga gcgtggtcat gccgtcggcg atcagcgcgc gcacggtcgc ggtccagtgc
     5821 acgggactcg cgagctgggt cacgaggatc gcgcgaatcc cgtccggatc gtcgtgacgc
     5881 ttcgaatcga ccgcgctgta gtacgggatg cgcggcaccg cgatcgccac gccgcccagc
     5941 cgctcacgca ggcgctcggc ggcgccacgc atcaggctgc tgtgcgaggg cacgctgacc
     6001 ggcagcagga ccgcgcgctt ggcaccccgc accttcgcgg cctcgatcgc ccgcgccacc
     6061 gcggcgctct cgcccgcgat gacgatctgt cccggcgagt tgtagttgac cggctcgacc
     6121 actgcgccct gggcggcctc gcggcaggcc gcctcgatgt cggcgtcctc gaggccgagc
     6181 accgcggcca tcgcaccgct cccgagcggc acggcctcct gcatcgcacg accacggaac
     6241 tgcacgagtt cgatggcggc accgaaatcg agcgcaccgg ctgcgacgag cgcggtgaac
     6301 tccccgaggc tgtggccgga tacggcgttc ggcaacgccc caccacgcgt ctgccaggcc
     6361 cggaaggtcg cgatcccggc agccagcatg gccggctggg tacgctcggt ctcgttcagg
     6421 cgttcggcag gcccggccgc aatgagcgac cagaggtcgt agccgagcac gctggatgcg
     6481 gctgcgaagg tctcgcgcac gatgggctcg gcctcggcga gggcaccgag catgccgacc
     6541 gattgcgaac cctgaccagg aaatacaaac gcgataccca tgtgtcgtcc ggtggtgtcg
     6601 gggcgcggat tataacggct cgcgtcgcga gggcaggcaa agtgcgatga cgagcccgcc
     6661 gaccgcacca tacagatggg cctgcacgac caccggcgcg tctcccgcaa acggcagcgg
     6721 catgtactgt tcggcgagga gcttcccggc cagcaccaac gcgacaatcc agccgacgtg
     6781 ttcgcgacga cgcagttgcg cgagcgcgcc ggccgccatc actccgtgca gccagcccga
     6841 ggcgccgacg taccactcga tacccggtgc gccgaaccac aggccggcat cgatcgccag
     6901 catcgacacc cccacgatgg cgagccaatg cacgggccgg tagtcgcggg cgaacagcgc
     6961 ccacacgagc gcgcaccccg cgccattgag cagcgcatgg cgcagatcga ggtgcaccca
     7021 gtgggcgctt gccagtcgcc agaactcgcc ggccgcgacc ccggtgcgct cgtagcgcca
     7081 tgcgaggcgc cccacctcac cagccagcga tggcagcagc agcaacgcca ggacgcaggc
     7141 cagaccaaga ccgtagcgac cgtcgccgtt gagcgacgcg agcaggcgcg acggagccgg
     7201 gacagccgtc atggttcaga cacggtcatt tgctatgatt atgaacgata tgaaccagcc
     7261 tgcccgttcc cgcggcttca cgctcatcga gatcatggtc gtggtcgtga tcatcggcct
     7321 gcttgcggca ttcatcgccc cgcagatcct cggtcgagtc gacgatgcga agatcgtcaa
     7381 ggcgaagcag gacatccagg cgctggaaac ggcgctgacc atgtacaagc tcgacacctt
     7441 ccgctatccg ggcaccgacc tcgggctcgt ggcgctcgtg cagaagccgg ccgatccgca
     7501 ggtgcgcaac tggcgcgaag gtggctacct gaagcgcgtc aacaaggatc cctggggcaa
     7561 cgactaccag tatgtgtttc ccggcacgcg cggcggcgag tacgacctct actcgctcgg
     7621 tgccgatggc gagccgggcg gcgaaggcat caatgccgat atcggcaatt ggaacctcga
     7681 tcgctaggac ccggccgccg gggccccggc catgaagcac gcgcgcggct tcacgctcat
     7741 cgaaatcctc gtggtcgtcg tgatcatcgg cgtgctcgcc gtcggtgcgg tgctcgcgat
     7801 cggcacgtcg aagcgcgacg acacgctcga gaaggaggcg cagcgcctcg aagcgctgat
     7861 cgcctacgcg cgcgagcacg ccgagctcgc gacgcgcgag ttcggcctgc gcatcgaggc
     7921 cgaccgttac ggcttcctgg tctacgaccc tcgccgcggc atctggcaca gcgcagatgc
     7981 cgacgaggtc ctgcgtgaac gggcccttcc ggtgggcctg cgggtccgcc ttcgcatcga
     8041 gggtcgcgat gtgctgctgc gaccgctgaa gaccggcgag ccgctgatgc cgcagttgat
     8101 gctgttctcg aacggcgatg tcacgccctt cgaactcatg ctggatcgcg cgggcagcga
     8161 actgcgcgcc acgctggcga gtgacgaagc gggcgccatc aaactcactc tcccgcagga
     8221 tgcaaggccg tgatgcgccc gcgcgggttc acgctgatcg aggtgctggt cgcgctggtg
     8281 atcgtcgggg tcggtatggg cgcgctgctc gcgacactga cctccgcggc ggattcctcg
     8341 atctacctgc gcgacaagac cttcgccgag tggatcgcgc tcaaccgcat cagcgaacta
     8401 cggctcgccg cgaaggcacc ctccaagggc aagtcgagcg gccagatcaa cttcgtgggt
     8461 cggcagtgga actacgaaca ggaggtcgtc gaaaccgaca tccccgggtt gttgcgcatc
     8521 gacgtgagcg ttgccgacac cgcgacccag gcggccggca acaccaagcg caagtcctgg
     8581 acggtcacgg ccagcggcgt cgtgggtgat gcgctgctgg cgccgaacgg ccaggaaccc
     8641 gactggaacg gcggcccctt ccccggcgag ccggtccgcg gccgccagga cgggcaggaa
     8701 ccgccgccgg acgatgagtc gacccctgtc ccaccgccgt ccggcagccc gctgcccacc
     8761 tcctgatgcg tccgcgcggc ttcacgctcg tcgagttgct ggtggccctg ttcatctcgg
     8821 ccatcatgtt cgcgatgggc tacggcgccc tccagcaggc gctgtccaat cgtgaagccg
     8881 tgcaggccaa ccaggatcgg ctgagcgcac tcgtcaacac gatgcgcatc ttcgcgcagg
     8941 atttcggcca gctggcgcca cggcccacgc gcagcctcgt cggcgacggc gccgacccgg
     9001 cgttgcaggc ggacgggcgc tcggaggcgc tcgcgacctt cacgcgcttc ggctgggcga
     9061 atcccgcggg gatccagcgt ccggccctgc agcgcgtgcg ctacgtgatc gaggacggca
     9121 cgctctatcg ggagcactgg cacgtgctcg acccggtgct cggcgcgcag cccgcccgac
     9181 gcgcgctcat cgacggcgtg cgcacggtgc gctttcgctt catggagagc gggcgcagct
     9241 ggaccgagca atggcccggc atcgtcacgg ccggcgcacc gcgtcagccc cagctgcgac
     9301 gccggccgat cgccgtcgag gtcgtgctcg acgtcgaaga cttcggcacg atcacgcgca
     9361 tcttcgaggt gccgggatga agcgcgagcg cggcgtggcc atgctgaccg cgatcatcct
     9421 cgtcgcgctc gccacgatcg tcgcggcgag cattgccttc gtgagcggca tgacagcgag
     9481 acgcacgagc ggcgcgttca tgttcgacca gggcatacag ttcgcgggcg cggccgaggc
     9541 gatggccgcc tacgtcctgc gcgaggatcg caaggacaac cagaccgacg actacaccga
     9601 aacctgggcg caaccctacg gacccgtgga actgatcgag ggctcgggca tctggctcga
     9661 agcgcggctc gatgacgcgc agggacgctt caacgtcaac aacctggtcg ataccgaggg
     9721 cgtcgtgcgc ggcgaggcgg tcgagcagtt caagcggctg ctcgcgatgc tgcagctcga
     9781 agagaaatgg gccacgctgc tcgccgactg gatcgacgcg gacgatcaac cgaacggttt
     9841 cgaaggcggc gaggacaccc tgtatctcgc gcagagcccc gcctaccggg cgccgaatgc
     9901 accgctcgtc agcaccagcg agctgctcgc cctgccgggc ttcggcaacg agcgctacct
     9961 gcggatcgcg ccctatatct ccgcgttgcc acccggcaca ccgctcaatg tctgcaccgc
    10021 gccgggcatc gtcatcgacg cgatgaccga ggagcgcgag tacggcctcg accaggaacg
    10081 gctggcaaag tcgcgcgagt cgggctgctt tccgaccaag gccgacctgc aggccaagat
    10141 cgacgctgag aagtggatca cgctccagta tcagatcagc gacagttcgg agtactttcg
    10201 cctgcggagt ttcgtcggta ttggcaccat ggagttggcc ttgtacagtc tcctcaaacg
    10261 cgaatcgacg ggcgtccgac cgattctgcg cacctacggt acggattgac ccatggctga
    10321 ctggttcgtt gtgcgtctgc cccgcgcgag cggcgatcca gccagctgga tcgcggtcga
    10381 cgagcaggga cacatgctcg cggcgcccgg cgccggcacg ctcgcgcaag cagccgcgca
    10441 ggctgcggga cgccgtgtcg ccgcgctcgt tcctgccgac cacgtcctgc tgaccgacgc
    10501 ggccctgccg gccaaggcgg gcgcgaagct cgcacaactc gtcccttacg ccctcgagga
    10561 gcaggtcgcc gacgacatcg atgccctcca tttcgccgtc ggtcgccgca atcccgaaac
    10621 gggccgcacc agcgtcggtg tcgtcgacag gtcgatcatg cagtcagccg gcgaggcgct
    10681 gctcaaagcc ggcattgcag cggttgcgct ctacagcgaa gcgagcctca tcgcgcccaa
    10741 tcccggacag gttgtggcct ggctcgacgg cgacacgctg gtggtcgcct cccctgcgcg
    10801 tgccccgatc gtcatgccgg tgacttcact gcgcgaggtg ttcgagttcc tcgccagcac
    10861 ccacgcctat ggggagggcg tactgcccgc cacgctctcg ttgcagctcg tcgcgaccag
    10921 cctcgactgg cagcagcatg ctggcgagct cgatttcctg caggaacatt tcgccggcgc
    10981 ccgcgtgcaa ctcctgcctc agggctcgct gccctggctc gccagccaga tccgggcggc
    11041 cgcaccgctc aacctgttgc agggcgacta tgccgcgcgc atcgccggtg gcgcggagtt
    11101 gcgccgctgg cgcgtgcctg ccgcgctcgc cgccgcactg ctgctggtat tcgtggccgg
    11161 caaggtctac caggtccagc gactcgacgc cgccgagcgc gcactcgatg ccgagatcga
    11221 ccagaccttc cgcatggcga tgccgggcgc cgcggattcg accgatgcgc gccgccgcat
    11281 ggcgcagcgc ctcgcgtcga tgcaggacgg cgtcggcagc gccgggctgc tgcccgcgct
    11341 cagcgcattc gcctcggcac gcgccgccgt gccggatgca agcctgcacg gcatttcatt
    11401 gcgcgatggc tcgctcgact tgcgtgtgcg cgccgccgat gccgcgagcc tcgaccagat
    11461 caaccagcag ctgcgcgcca gcggctatac caccgaactg tcgagcggca cggccgaaga
    11521 gagtggctac gagggccgca tccacatccg gctggcgggg gcatgatgga agccgccagg
    11581 caatggttca atggccacgc agagcgtgag cgtcgcttcg tcatctacgg cggcgtcgcc
    11641 gccattgtga tcgtgctgct gatggcggtg ctgcctctgc agcgcagtgc cgcggcgctc
    11701 gagcagcgcg tcgcgacgaa gcgcgccgac ctcgcctgga tgcgcagcgt tgcgccggcg
    11761 ctcgcaggcg cgggtccggt gtccgatacg ccgctcacgc aggagtcgct cgtcgtcacg
    11821 gtcgatcgca ccgcgcgtga atccagcctc ggcgaggcgc tcgcgcgctc ggaacccgca
    11881 ggccagggcg gccagcgcgt gcagttgaac ggcgcgcgct tcgacgtcat ggtggcgtgg
    11941 ctcgcgcgcc ttcgccagca gcacggtatc ggggtcgttg cggcgaccat cgagcgcagc
    12001 ggggagcccg ggctcgtcaa cgccaccgtc gagctgaatg cgctgtgaag ccgctgcgac
    12061 tcgcggcgct cgtcgccgcc gcgctgctcg tgaccttgct gctgcgctgg ccgttgtcct
    12121 ggaccgtgca ctggctgccg gattcgctgc actgtgccta tcctgccggt agcgtctggc
    12181 ggggccgctg cgcggcgctc acggtcgagc agctgcgtct gggcgaaacc tcctggcagc
    12241 tgcgtccgct cggtctgctg cgcggcgcgc tcaccgcgag cgtgcagtcc cggcaaggtc
    12301 ccgaccggct gatggccgac atcgaacttc ggccaggcgg tcgacgcata gcgcgcaatg
    12361 tcgaagccga actccgactc ggcgaaggct tgctgcagcg gagcgccatg ggtctcaccg
    12421 ggcagctgca actgcagctg tcgcgagtcg tgctcgatga gcgcacagtg cgtgagatcg
    12481 aaggcacggt caccgcccgc aagctggtcc agggcgcgag ccgcgccacc ccgctcggcg
    12541 actatgaact cgtgtttcca cccggatcgt cgcaggcgag cctgcgcgat ctcggcgggc
    12601 cgctcggcgt caccggcact ctgaccctga ccggcgagcc gggctatgtg gtcgaaggat
    12661 tcgtcaccgt gcgcccggct gcgccgcagc cgctcgcgcg cacgatcgcc atgctcggca
    12721 cgcccgactc cgcgggtcgt cggccgttct ccttcgccgg cacctattga tcgtaagcgc
    12781 gaaagatgcc gagcaaggga aagtagagcg cggtgcggac gggctccggg ccgagcgcgc
    12841 ccgccagcac ggcatagcgc gcgagctgcg ggcgatagcg ttccatctcg ctcgccagga
    12901 acgtttcgag gtcgccgccc tcgtgacggc tggtcttgta gtcgatcacc cagcgcactc
    12961 cgtcggcgtc gacgaacgtg cggtcgaaca gcacgttcac gatgcggccg tccacgagac
    13021 ccgtcagcgc aagttcggtc gcaccgtgcc gatggcggga atcgaacagc cactggccgc
    13081 gcggatcggc ccatgtccgt cgcagcgcct gcatcacggt cgcagtcgcg gcatcgagtt
    13141 cggattcggg cacgccgagc cgcgccagca gcgtgcgctg cgcggcggct ccggcttcga
    13201 tcccggcggc tgaagcgggt agcgcgcgtc cccagctttc gagcgcggcg tgtaccgccg
    13261 tgccgatgtg gcgcgccgtt gcacgcaccc agctgaactc gggcgtgtcg agcggctcgc
    13321 gggcgaggaa caggctctcg gtcgtgacgg cgtcggccag cgcagcggcc tgccagtccc
    13381 ctggcaggcg ctcgagccgc gtcgcgaccg aagcatcgat ggcggcattc gccggcccgg
    13441 tatcgactac ttcgaacctt ccggccggga ccggccacgc agctgcgagc atcgtcccgc
    13501 ccgccggcct cgggtcatcg tcgtccttca cggccacgcc ggcgatccag tgcagcgacc
    13561 gccgcgcacg tgtggccgcg acatacagca atcgccgctg ctcgtgcgcg cgcctcgctc
    13621 tgccgagttg cctgatgtag gcgcccagcc ggtgctcgtc gcgctcgccg gcggcgacga
    13681 tgggcgacat caacaggtcg gagtcccggt cggtgcgcgg caggtcgagc cagcgcagca
    13741 agggctcctc gtcggagcgg ggacgtcgac ccagccccgg caggatcaca tgatcgaact
    13801 cgagaccctt ggcgcgatgg atcgtcatga tctcgatcgc gcccggcgcg gcactgtcgg
    13861 ggcgcgcata gagatccgcc agcatcgtgt cgatcgatgc gctgccctgc cactcacccc
    13921 tcgaccacgc cgcatcgagc gcgcggaaga attcgcgcgc agcatcgagc tcggcaacgg
    13981 ggtagcactc cggcccgccg agctgcatcc ataccgactc gagcgcctcg cccggcgcgc
    14041 cctgtcccgg cccggcgaac gcgcggccga cgacttcgcg cacccgcgca aggcgcgacg
    14101 cctcgtccgg agccagctgc gcggtccgtt ccgcggactg catggcctcc cacacgagca
    14161 agggatcctt gcgaccggac agcacgcgca ggctcgtcaa tttcaggccg caccacgggg
    14221 cccgcaacag cgtgagccac gccgtgcgat caccgggatg cagcaaggcg cgcagcagcg
    14281 cgacgaggtc gcgcaccgcg gggacttcgg cgagcggcac gagatcgaca ccgcggaccg
    14341 cgtaccccgc cgcgacgagg cgcgccgaaa tcggcacggc gtgggctcgg gccagcacca
    14401 ggatcgcgat gctcgcctgc ggttcgcgtt cgcgcaacgc acgcacgcgc ccgagcaccg
    14461 cacgcgcgtc atcgtcaccg tcgccgttcg cgagagcgag cagcgagacg gcctcggccg
    14521 accccggcgt ctccgccacg gccgccgcac tgggcgagta gcgcaccgca cctgcgcgca
    14581 ggtcgtcatg atcggggaac agtcgcgcaa acagctcgtt gttgaagtcg accagcgcgg
    14641 gccgcgagcg gaaattgcgg gtcaggcgca gcgcttcgag cttcaccgga ccgatgccgt
    14701 gatcacgcgc gcgcaggaac aggcccacct ccgcttcgcg gaactggtag atcgactgca
    14761 tcggatcgcc gacgacgaac agcgtgcgac cgtcgcccgc ggaccagtcc tgcacgagca
    14821 tctcgagcag ttcgaactgc gcaatcgagg tgtcctggaa ttcgtcgacg aggatgtggc
    14881 gcagtgtcga tccggtgcgg atcgcgaggt cgctggcgcc gccttccgcg gtgagcgcgg
    14941 cacgcgcggc gccggcgacg tgcacatgat ccacccgacc cgcctgggcc atctcgagct
    15001 gcaggtagcg cgccacccag gtcaggactc gggccagcgc atcgatcgcc tcgccatccg
    15061 cctcctccag tccaggcggc ggaagctcgc gcgtctcgcg caggagctgc tccgccccgt
    15121 cgatgcccgc cagctcatcg agcacgctct tgagcgctga cttggtcgcc gcatcccggt
    15181 acgcagggtg gatacgcgca tcgacgcgtt tgcgcagcgt gccggcctgg gtcagcacgg
    15241 ttgccgcgaa cctgtcccag gccgcgaacg ccgcgggcga ggcatccagc gcgccgacat
    15301 cgggcagtgc gctgcccgcg gcgcgcagcc cgcgcggcaa ccgggccaca ttgcgggcca
    15361 gctgctccgc cacgatgcgc gtgagcgagg catcgatgcg cgcgcgcagg tcgccggtat
    15421 cgctggtcag cacgtggggc agccagtggg cgcgcctggc gagcatcccg gcgagcagcg
    15481 actccacctt gcgccattgg ttgtcgagcc gatcgaacag caggtcgagg tcgcggcgca
    15541 tatcgggctc ggcctcgcct tcgagcagcg cgcgccgcgc cgcgcgctga tagaggtccc
    15601 ggggccgctc cgcgatactg ggcgtcgtgc cgctgcctgc gacaagcggc agctgcgcgg
    15661 cgagcgaata gttgaacgcg tcgatcgtca ggatgcgcag tcgtgaggcc tgggcttcga
    15721 gctgccagcc gcgctcgcgc gagcgcgcat gcacgccatc ggccagccgg cgcagcagcg
    15781 gcgcctgcgg gtgcgcggga tcgatatcgc cggcgagcgc cgcgagtacg cgggcgcgca
    15841 tctccgctgc cgccttgcgg gtgaaggtga tcgcgagcac ctgctccggt tcgtccacca
    15901 ccgcgagaag cgccagcagg cgttgcgtca gcactgtcgt cttgcccgat ccggccggcg
    15961 cttcgagcag caccgaggta tcgatgcgga tcgcgtgctc gcgcgcgaac gtgtcgaggt
    16021 cggcgagtcg ggatgcgggc tcagtcacct gccgcctcgc cgtcttcgaa ccgtgcccgt
    16081 tcaccgacgc ggcacagcga accgaggtgg cagtagcggc aggcggactt cgccggggtt
    16141 accgccgcct cgccgcggag aaactccgcc gcgagaccac gcaatcccgc gagccaggcg
    16201 cccacctgcc gcgcccatgc ggcgtcatcc gtctcgccgg gattgacctg cagcacgggc
    16261 accttcggca acgacacgtc cggcgccgtg atgccgacgt agcacatgcc gtgccgggtg
    16321 acatgcgcgt tcgcgagggc gcgcacggcc tcgcccgtcg ccgagagata agtgagcagc
    16381 tggacggcct gcggtcgctc gccgtaccag tcgaagcgtt gcggcttgcc gctcttgtaa
    16441 tcgatcacgg cgcgtccgcc atctgaaagt tcgtcgacgc gatcgatgcg cacggcgagg
    16501 cgtgcctcac cgagagcaat gagtttctcc tgctccattt cggcgacgcg gaaggcggga
    16561 cgcgaatcct cgagcacggc gaaatccacg atgagcgcaa ccagcctccg cgcctcgcgc
    16621 tcgcgcgcgg ccaccgcggc ctcgccgcca cagagcggta gcggctcccg ggtctcatcg
    16681 accacccgcc gggcgagcgc acgacgctgc gccggcggca gcgccgcaag ctgcgtcgaa
    16741 tcccccacct cgcgccagaa agcctcgagc gccgcatgca gccagcggcc gcgttcgtcc
    16801 ggctccacac ctagacgcaa ttcgtccagc ggctcggcac cgagttgctg gcgtgcatag
    16861 gcgcgaaacg ggcagtcggc ctggtgctgc aggctgctcg tgcctcccgg cagcggttgc
    16921 gccgcgggcc acggagtgcc cgttccatcc gccagcacct cgagcgtcag cgggcgtcga
    16981 attctcaacg gcaaccacag gctcgcggtc cgatcctctg gcgcctcgga cctgggccac
    17041 gcagccagca gcggactggg agaaacgtgc atgtccccgg ataccgtcgt ggcgctgagc
    17101 acgagatgct ccgtcgccgc gcgccatgcc gcgagagact gtcgggccgc ggcaagttgc
    17161 ccggcgccgc tcgccgcccg cacacccgct tcgcgcaacg cgggcagcgg aacgaaagga
    17221 tcggtcatcg cgggcgcggg ccacgcgtcg gcggtcaggc ttgcgatcca gatgccgtcg
    17281 tactgcgcca ccggatcgtc gtgcgtcgcc gcgatcgtga cgagggcatc gccggtggca
    17341 ggctggtaag tcgtccgtgc ggcaagttcg cgcagggccg ccagtgctgc gtgcgcatcc
    17401 agcctgccgg cgacggcagc cacggcaccg aattcgtcga gcagcgccac gaaacgcagc
    17461 caggtctgct gccccgcgct gtcgagtccc gcgggctcgg cagcagcgcg cagcgccgca
    17521 agcgcgccga tgaaacgctc cgcccagccg cgcgcgtccg cggctcccgc cgcaagcacc
    17581 gcgagcgccg cacgtacccg ctcggccagc gtcgccgcgc ccgcaagacc accggccgca
    17641 gcccggtcca gcaaggcgag gctcgcgcgg gcatccgcct cgatcggtgc atgccggcgc
    17701 caccagatgt cgaggcgcgc ggccgcctcg gctgccagca cgccgcaggg actgcgcagc
    17761 caagtcgtga aggcgtcgaa ctcgagcccg tcaatgagcc aggcaaggcc cgcaagggat
    17821 tgctgcacga tcgcctggcg cgtgagcggt acgccacctt ccacggcaac gaggccgtca
    17881 ttcgagtcgc cggagaattt cgaggcgggt gcgagcgtgg cgcgaatctg cgcggccagg
    17941 gcttcgcgct gctcgaggcg ggtcgccgca accaccagca cgcgcgcatc ggcatcgcgc
    18001 tcgagccggc gcaggcacca ggcggagata cggccgattt catctgcttc gtcgcccgcc
    18061 agcacgacga cgggcttcgc gcgcggctcg ccgacgcccc accattcgcc gccctgcccc
    18121 ttttcgcgcc gcgccgcatg gatcgcccgc agccgcggca cttcgagcgc ggcaaagcct
    18181 gcgaaatgaa ccgatcgcgc gtccgccagt ccgtccatga gcgcggcgat gttcgcgacg
    18241 gcgtctgcgc ccagcctgcg cctggcctgc gagacagcgc gccgcacggt atcgagcagc
    18301 tgcgtctcga gcccgccaat gatgcgccag cgcgcaatgt ctatgccgtg ctcggccgcg
    18361 agccgatcgg cacgccgcaa cgaatccgcc aaggccgcag ttccgccaag cgcggtatcc
    18421 tgcgccgcgc gcgcagtcgc ctcgcgccac agccaccact cctccgcagc cgcgagcggc
    18481 cgtggcaggc tggagtcacc gtttgcagcg cgcgtcactt ccctcgcgag ccacccctcg
    18541 cccgagagca cgtctgaggt cggccatacg acctccccgc gcgcaagcgc gcggcgcgcg
    18601 tgcgctaccc gcagcgcgtg cgctcgctgc ggtgagggca cgacgaccgt gtccccacgc
    18661 tcgattgctg tcgaaatctc gtcagttact gtaatcaaat caggcgatta cacctatttt
    18721 atataaatta cttgaagcga gccgcagcgt cgacgacctg cccggtaccc gcgcccgatg
    18781 gcccacccgc ggcgtacgtc gcgagcagct cgttcatgcg ctggaacacc gtcttgagtg
    18841 tcgcggcatc cggcaggttc gtaatgcgga aatgatttcg atacggcacg ttgaagctga
    18901 cgcccggggc gacgagcacg tgtttctgtt cgagcaggtc gagcgcaaaa gcctggtcgt
    18961 cgaaacccgg catggcgttc gaatcaacgc cgacgaaggc gtagagcgcg cccagcgggg
    19021 cctgcagttg caggtacggg ctcgcagccg ccgcatcgag gataaccgcc cgcgagtcgt
    19081 gcaggcgacc gcccggcgcg gtgagatcct tgatgctctg gtagccaccc aatgcggtct
    19141 gcacggccca ctgcgccggc acgttgctgc acagtcgcag cgacgacagc agctcgagcg
    19201 cgttcaggta atcacccgcc gagcgcagcc gtccggagaa cacggtccag ccgacccggt
    19261 agccgcaggc gcggtagacc ttcgagagtc cggacatcgt cgcgcacagc gtgtcgtgca
    19321 cgagcatcgc catcggcacg aacgtggcgc cgtcgtagac gatctggtcg tagatttcgt
    19381 cggccagcac cacgagcttg tgcttctcgg cgatgcgcgc gaggcgctcg agcaccgcgc
    19441 gcggatacac ggccccggtc ggattgttcg ggttgatgat gacgatggcg cgggtgcggc
    19501 gcgtgatcag cgcctcgacg gcatccggat ccggcacgaa cccgtcctcg ggccggcagg
    19561 ggtagtgcac cgccctgccg cggttcaggt tcactgccgc ggtccacaac gggtaatcgg
    19621 ggctcggcac cagcacttca tcgccgtcgt tcagcatcgc gcgcagcacg aggtcgatca
    19681 gctcggacac gccgttgccg atgaatactt cctccgcgct gacgccctgt acgccgcgcg
    19741 cctgctgctg catgacgacc gcctcgcgcg cgggaaaaat gcccttctgg tggcaatatg
    19801 cctcgctcgc gcccagattc tcgatcatcg cgaggcgcat ggtctcgggt gcgcggaaac
    19861 cgaacaggcc gggattgccg atattgagcg agatgatttc gtgcccgaga cgttccattt
    19921 cgtgggctcg acgggcgagg cggccacgaa tctcgtagcg cacattacgg aggctgtcgc
    19981 tcgggcgaaa tgtctcggtc atggcgcgag catagcatcg cgccccggat cagcggatgg
    20041 cacctgcggc acgcaggcgt gccagttccg ttgaatcgag acccagttcc gcttcgagga
    20101 cggtgtcggt gtgctcgccg agcagcggcg gtgcgctgcg gtactcgagc ggcgtgcgcg
    20161 agtagttgat cgggttcttc acccccggca cggtgccggc ggtcgggtgc ggcaggtcga
    20221 tgcgcatgcc ccgatggacg atctgcggct cggcgaagac ctggccgatc tcgttgatcg
    20281 gaccgcaggg cacgttcgca gcattcagcg catccagcca ccagcgagtc gggtgcgcgc
    20341 gcagcgccgc cgccacgatc ggcacgagtt cggcgcgatg tgcgacgcgc gccttgttgg
    20401 tggcaaagcg cacgtcagcc gcgagctgcg gctgctgcgc aaccgaacac catgcggaga
    20461 actggcgatc gttgcccacc gcgagcatca ggaagccgtc ggacgtcgcg aacgcctggt
    20521 agggcacgat gttcggatgg gccgtaccct ggcgacgcgg cgactcgccg gtgaccagaa
    20581 aattcatgtt ctggttggcg agccagccaa gttgcgtgtc gagcaacgcg agatcgatgt
    20641 actgaccgat cccggatgcg cgccgctcgt acagcgccgc gatgatcgcc gtcgtggcgt
    20701 acatgccggt catcaggtcg acgaccgcca cgccgacttt ctgcgggccc ccgcccggct
    20761 cgccgtcggg caggcccgtg atgctcatca ggccacccat gccctggatc atcgcatcgt
    20821 agccgggctt gtcggcgtca gggccgtcct ggccgaatgc cgatatcgaa cagtagacga
    20881 gcccggggca ttcggccttc aggtccgcgt acgccaggcc atacttcgcc agcccgccga
    20941 ccttgaagtt ctcgaccagc acgtcgcagc gacgcgcgag tgcgcgcacc aggctcgcgc
    21001 cttgcgggtt cgagaggtcc acggcaaccg agcgcttgcc gcgattggtc gacaggtaat
    21061 acgcggattc cgtggtatcg cgaccttcgg cgtcgcgtgc gtacgggggc ccccactggc
    21121 gtgtgtcgtc accgccatcc gggcgctcga tcttgatgac atcggcgccc aggtctgcaa
    21181 gcgcctgcgt ggcccaaggc cccgccagca ctcgagacag atcgagcaca cgaatgccat
    21241 gcagaggacc catgttcacc caccggccgt gcggccgccc gcgtagttat ttcttgcggc
    21301 ggcgcctgcg cgatttgtgc gcatgctcga cctgcccgtc gccatgatgg tgcggcatgt
    21361 cgccgtgctc atggccgtgc gggaagtcga agtcaaccgg atggttgtgg ccgtgggtat
    21421 tgatccactt gtacaggtcc ggcatccagt gctggatgcg gctcgcggcc cgctcgcggt
    21481 tctcttcgac gttggtccac ggattgaagt ggaagtggtt gtacaggcgt gaatcggtgc
    21541 ggccgagcgc cagcgtaccg agcttgcaac cgaaggcacc gtggttgatg tagggcggcc
    21601 gatagaagta gccgccggtc ggcaggtcac cgaactgcat catccaggaa gtgccccaga
    21661 tgtggtacgc ctcctcggcg cagtcgtggt aggagatgtt gtcctgccag aagttcggcg
    21721 tcatgcagta caggaacgtg aacgcctgcg tgcgttcatc gaaatgcagc accttgacca
    21781 ggcagccggg cgccgtggcc gggaagacat tggtcgaggt ccactgcatg tcgtcgtagg
    21841 cattcaccgc cacgaacttg ctgcgttcgg cacgcgggtg atcggaatcc gattcgacga
    21901 aggacggctc gccgtagttg tagtacagca ggcccgtagc gccgcgcgac gaggaaagcg
    21961 ccccgagcgc gaccccggcc ggcaccatga aatagctgcc cggcccgtgg accttgtcac
    22021 cgacggtgat cgagcccgtc atgatgaaca gctcgtactc gctgtagctc atgcccggcg
    22081 gttgcttgta gccaggctcg aacatcacgg tcatcgtgca tgcgccgttg tccgggtcga
    22141 gcgagagcat cttgtagtgc atgcccttcg ggaaaccggg catcgtgaac ttgcggaact
    22201 ggacgtcgcg gtcgacgaag ggctcgatat gtgggcgtgc catgtgagga ctccggcgct
    22261 ggcgcgcttc agaatacgac gcggaacttg cgcttcgggc ggaccggaat ctcctccgga
    22321 ctcaccttgc gcatcatatt gaagtgcacg tgcacaccgc gcgtctggat gcgctcgagg
    22381 aattccgcca cccatttgtc gcgcttcggc gcgagcgcat cctgctcggc gacgatgctg
    22441 ttgtaacgaa ccttctgctc ggcgatctcg cgcttgagcc aggcttcgac ttcacgactg
    22501 cgcttgtgcg ggatggattt cacggtgcgt ggcctgcaaa atgattagac gcggtcttat
    22561 accgcgcgcc ctgcaccttc acaaccgtag caacccctac aaggcgcgcc ccatggaggc
    22621 gaccgcgtag cggccaccgg agaaacgcat gctcgatgcg atcatcgccg tcaccctctc
    22681 ggcacccgac ctcggcgcgg ccgaacgcgc gtaccacgca cagctcggct accaggtcgt
    22741 ccagcgggcg caggtctcac gctcgctcgc ggccagctgg gatgcgcgtg ccatgcgcgg
    22801 gcgtgactac gtgctgatgc agcccgcgag cggcgagccg gtgtacctgc gcatcgtgtc
    22861 cgccgaggcg cccctgcccg gttacgcgcc gctgcgtagg tacggctgga acgccaacga
    22921 gatcctggtc gaggatcccg tcgcgctcgc gtcgcgcctg cgcgaaccgt tcaaggagat
    22981 cggcaaaccc cggccgctgg atttcaaccc cagcatcatc gccatgcagg tcacggggcc
    23041 cgccggtgag gtcaattact tcactcgcat cccgcccacc ggatcggtat tcaagctcgg
    23101 cagcgcgaag tccttcgtcg accgcacgtt catcgtcgtc gtgggcggcc ccgacgtcgg
    23161 cgcgctgcgc gcgttctatc gggatgtgct gcaggccccg acgaccgacc cgcagcccac
    23221 gaaaatcaac gtgctgcaga atgcctacgg actgccgccc gacgccgaga cactcatggg
    23281 gatcgtgcag ttcccgtcga gcttcctgat cgagctcgac cagtacccgg aaggcgcagt
    23341 ggaccgcccg atccggcgtg gccagctgcc gcccggcatc gcgatggtga ccttcgcggt
    23401 caagagcttc gacaccatgc ggctgccgtg gaaggtgaag ccggccgtac ggagcgaggc
    23461 gccgtacaac ggccgacgcg ccggtctcgt gattggcccg gcgggcgagt ggatcgaact
    23521 ggtcgagacg ggcggatcca atccgggctc cggaccctga agctagccca ggcgtcgtcg
    23581 cgccacgacc gggacggccg cgaccagcgc ctgcgtgtag gcgtgctgcg gcctgtcgat
    23641 cacctgcacg gcgtcgcccg cctccacgat gcgtccttgt cggagcacga tgatgcgccg
    23701 gcacagccgg cgcacgagcg cgagattgtg gctgacgaag accagggtca ggttggaatc
    23761 gcggtgcagc gattgcagca gctgcatgat ctgcgcctgg atcgaaacgt cgagcgcgct
    23821 caccggctcg tcacaggcga gcatcgccgg cccgagcatc atcgcacggg cgatgcagat
    23881 gcgctgacac tggccgccgc tcagctcgtg cgggtagctc gccagcacgc cggcgggcat
    23941 gccgaccaga tccagcatct tcgcgatcgc ctcgtccacc gcggcgcgcg cgagttccgg
    24001 ccggtgtacc tgcagcggct cggcgagcag ggcgcgcacg cgcatgcgcg ggtcgaggct
    24061 cgccagcgga tcctggaaca cgggctgcac ctggcgccgc aacgccagcc atgacgcagc
    24121 atcgaagctg gcgagatcgc gcccgtgcca tgcgatcgag ccgtccgcga tcggcatgag
    24181 gcccagcaga gcgcggagca gcgtcgactt gccgctgccc gactcgccga cgatgccgat
    24241 gctgtcaccc gcctcgacat cgaagctgat gtcgtgcagc gcgacgaaac cagccgcgcg
    24301 cccgacgccg cgcccgcgcc cgtaacgcac gccgacttgc gacacgcgca gcagcggcgc
    24361 gccggtgctg gcgctcatgc cgacaccggg agatggcacg cgacgccccc gtccagctgc
    24421 ggctgtctga cacggcagcg ctccgcggcc cgcggacagc gcggatggaa tgcacagccg
    24481 gcgggcagtg cgcgtggatc ggggggttgt cccggtatgg cctgccaggg tgcaccgggc
    24541 ggatcgtcga ggcgcggcag gcattcgagc agggccgccg tgtacggatg ccggggtgcg
    24601 gcgaccaggg cggccgccgt cccctcctcc acgacccggc cggcgtacat gacgaggatg
    24661 cggtcggcga gccgcgcgat gaccccgaag tcatgcgaca cgagcgccat cgccatgtgg
    24721 cgctcgcgct tgagcgccgc gagcagctcc agtatcccgg cctgcaccgt gacatcgagc
    24781 gccgtggtgg gctcgtcggc gatcagcagc ggcggatcgc aggcgatcgc gatcgcgatc
    24841 gcgacccgct ggcgctgacc accggacagt tcgtgcgggt accgcgaaag gcgtctcgcg
    24901 gcatcgtcga tccggacctg ctcgagcagt tcgagcgtgc gcgtgcgggc cgcggcgccg
    24961 tgcgcgcgcc cgtggcattc gagtacctcg atgatctgct cgcctacggt gcgatgcggc
    25021 gtcagcgacg acatcggatc ctggaacacg aagcccgcgc gcgcaccgcg cacccggtcg
    25081 agttccgcct cggactttgc caacaggtcc tcgcccgcca gtcgcgccat accgcccacc
    25141 tgggcgtcgg atgccgtcag gcgaaacggc gcgagcagcg cctggctctt gcccgcaccc
    25201 gattcgccga tgatgccgac gcattcgccg ggcgcgatgc gcagcgaaac accggcaacc
    25261 gcgaccaggg gcccaccgcg cgccgcaaag cgcaccgcga gatcgcgcag ttcaagcgcg
    25321 ggtgcgggga tcgaggacat cgcgcaatcc gtcaccgagg atgttcagaa gcagcgtcag
    25381 cacgacgaga cagcccgccg gtgcgagcag cagccatggc gccgtttcca tgtcgcgggc
    25441 accatcgctg acgaggctgc cgaggctcgc cagcggctcc tgtacaccga ggccgaggaa
    25501 ggacaggaag ctctcgtaga ggatcatctg cgggacagtc agcgtggcat acgcgatcac
    25561 cggtccagcg acgttcggaa ggatgtgacg ccacaggatg cgagcggtcg gcacgcccat
    25621 cgcgcaggcg gcggtgacga attcgcgctg tcgcaatgcg agcgtctggc cgcgcacgat
    25681 gcgggcagtc gtgagccagc cgacgctcgc gatcgcgatc aggagcaccg gtatgctgcc
    25741 gcgctcgaac agcgtcgcga ggatgatgac caggaacacg tagggcagcg cataaagcac
    25801 atccacgatg cgcatcatta ccgcatcggt cctgccgccg gcgtagccgg cgattgcgcc
    25861 ccatagcagg cctatcgcga cactgaccaa tgccgcgacg aatccaatca gcatcgagat
    25921 gcgcagtccc tgcatcgtgc gcgcaaacac gtcgcgcccg agccggtccg tgccccacca
    25981 gtgcgcgtcg gcggcgccgg gcgcagcagc cagccgcgac cagtcgatgg cctcgggcgc
    26041 gtgcggcgag agcagcggag cgaacaacgc gagcatgcac agcgccagca gcaatgccgc
    26101 cgcgacgcgc agcgcgcgac cttgcggcag ctcaatgccg ccggatacgc gggtcgagcc
    26161 aggcatacag caggtccgcc agcaagttgc agatcagcgt gatcgttccg tagacgaggg
    26221 tcatgcccat caccagggtg tagtcccgat tgagggcgcc ctcgacgaga aagcgcccgg
    26281 ctccggggat gctgaacacc gtctcgacga tcaaggagcc ggtcagaacg aatgcggttg
    26341 ccggccccag ccagctgacg aggggcagca gcgccgggcg cagcgcgtgg gtcacgacga
    26401 ggcgccgcat gccgatgcca cgcgcgcgcg cggcgcggat gtagtccagg ccgaggacct
    26461 cgatcaggcc ggtgcgagtc aggcggatca cataggccag taccggaatt gcgagcgtga
    26521 cgacgggcag gattgcatgg cgcagcgtgc cggcgtccca gccggccgcg ggcagccagc
    26581 gcaggcgcac accgaacagc agcgcaagca acgggccgag cacgaaggtc ggcaatgcaa
    26641 tcgcgagcgc cgccagcagt gcgacggcgc gatcggtgag cgaaccgcgg cgcagcgccg
    26701 cgccgacgcc gagcggaatg ccgagcagca gcgccagcgc cagcgccgcc gcgcccagcg
    26761 tggcgctgat cggcaggccg gcaccgatca attcactgac cgagctgtcc ttgtgcttga
    26821 aggagggacc caggtcgccg tgcagcaggc cgcggacata gcgcaggtat tgggtcgtga
    26881 gcggcgcgtc gaggccgtaa gcggcttcga gattggcgcg cacttccggg agcgcaggcc
    26941 gctcgtcgtc gaacggtccg cccggagcga gacggatgac gaagaatgcc gccgtgacca
    27001 gcagcagcag tgtggggacc aggccgatga tgcggaggac ggcatggcgc atcatcgcag
    27061 cgacctcagg cggccgacac ggctgccggg gagactaccg gtgcgcgtac cttgagcacc
    27121 atggcgaacg cgatggcgac ggcgatcagc acgcagatca atccgaagcc cggcgtatag
    27181 ctgccgtagg cgtcgtagag caaggaagtg atccacggcc cgagcgcgcc tccgccggca
    27241 tcgagcacgg tgattgcgcc catgacccgc ccgagcgagc gcgaaccgaa tgcatcggcg
    27301 gcaatcgcct gcagcagcga gtaattgccg ccccagccga agccgaacag catgatggcg
    27361 aacatcacga gcgatttctg cagcgtcatc agcatcagcg cgccgccgag catgagcagc
    27421 aggctcagga tccagacacg cttgcggccg aggatgtcgg acagcacgcc agagaccacc
    27481 ttgccgatca gtcccagaat gaacagcggc acgaacagcg ccgcgctctc ggacggcgcg
    27541 aaaccgaggt cccgcgaatg caggaacagg ttgttgctga taccgaggat cgaatagaag
    27601 gtcgcgaagg cgccgaggcc gatcagccag aactgtctgc gcgcgacgat gtcgcggtat
    27661 gagagcaagg ggcccgcgag ctgctcgctg cccgccggac tgctcgcacc gtagggagcc
    27721 agtccgatcc gcgccggcca ttccctgatc gagaaccaga tgatcggcaa cagcgcgagc
    27781 ggcgcgagcg cggacatcga cgccgcatcc cgccattcgt aggtctcgat cagctgcgtg
    27841 aacacgctcg gcaga
//
LOCUS       DAOQVY010000021        27603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_2039_length_27603_cov_5.502505, whole genome shotgun sequence.
ACCESSION   DAOQVY010000021 DAOQVY010000000
VERSION     DAOQVY010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 27603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27603
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2039_length_27603_cov_5.502505"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PKN91_03500"
     CDS             <1..379
                     /locus_tag="PKN91_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="ABC transporter maintaining outer membrane lipid
                     asymmetry; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HNS27170.1"
                     /translation="RVALARAVVMDPELLIYDEPFVGLDPISMGVIVRLIRQMNRVLG
                     ITSIVVSHDVHELAAIADESYLLSEGRCVASGSPEELRVSDSEIVRQFLDGIADGPVP
                     FHFPAPGYREQLFGVNGARRSSR"
     gene            376..1143
                     /gene="mlaE"
                     /locus_tag="PKN91_03505"
     CDS             376..1143
                     /gene="mlaE"
                     /locus_tag="PKN91_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HNS27171.1"
                     /translation="MNRAVARDGMVTGMLRRIGAVGVFFGALLAQCVPALARPRLIVH
                     QIYNAGARSLIIVMMCGVFVGMVLGLQGFDLLQRFGSEDALGVAAALSLLKELGPVVT
                     ALLFAGRAGTSLTSEIGLMRATDQLTAMQMMAVDPMQRIIAPRFLGGVIAMPLLVAIF
                     NVVGLFGAQLVGVQLMSVDPGAFWSQMQGAVELNDVQEGFIKSLVFGTACSLVAVYEG
                     YHSEPTAAGVGLATTRTVVTSSVLTLLLDYILTAAFL"
     gene            1157..1666
                     /gene="mlaD"
                     /locus_tag="PKN91_03510"
     CDS             1157..1666
                     /gene="mlaD"
                     /locus_tag="PKN91_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HNS27172.1"
                     /translation="MRPNRALEIGTGLFVLLGFAALLFLTTQLPGSGARLGGPQGYVV
                     TARFDNIGDLKPGSPVTLAGVRIGRVAGIDFDPTDFKAVVTLEIEDRYSQIPDDSDAS
                     IQTAGLLGGKYVGIGPGGSETYLHAGSTIEFTQSAIVLESLVNKFFANFASGRDSKEA
                     PQDAQGETP"
     gene            1681..2313
                     /locus_tag="PKN91_03515"
     CDS             1681..2313
                     /locus_tag="PKN91_03515"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNS27173.1"
                     /translation="MTMTRKSIPMLLAAMLLAAPVLAADSVDASGPGELIQTAAQSML
                     TALDADREAYRQDPKKVERLVEEVLLPHFDAQYAARLVLAKHWRTATPEQRERFIKGF
                     YNSLLRNYGTALAEFTGDRLKVFPAQIAPDGERATVRTEVKRSNGERVPVNYTLRRTE
                     GAWKAWDVTIEGISYVKSFREDFGSEIDAKGLDAVIERLERGDSPVKKSA"
     gene            2310..2612
                     /locus_tag="PKN91_03520"
     CDS             2310..2612
                     /locus_tag="PKN91_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNS27174.1"
                     /translation="MIELVAIAPGRLALRGGLDFGSAVIAHQAGLAFFSQGDTREWTV
                     DCSGLTDANSAALAVLLDWLAAMHRSGGKLRYEGLPERVLAIAEISEVDELLAAGV"
     gene            complement(2617..3417)
                     /locus_tag="PKN91_03525"
     CDS             complement(2617..3417)
                     /locus_tag="PKN91_03525"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNS27175.1"
                     /translation="MPHWILVAVATLVLTGCTTQTLKPDPRDPLEKINRTISTVNDRL
                     DRAIGKPVATAYRKITPQLVQTGVTNFWSNATYPSTIINQFLQGKPLTGLRDLERFLL
                     NTIAGIGGILDPATAAGLDRNDEDFGQTLGKWGVPSGPYIIIPLLGPSSFRDGAGRIA
                     DRFADPVHFVDDWRWQWGLDGLRLLERRARLLPATEALKDVHDPYAFIRNAYFQQREY
                     VIQDGNVIDVPADEGIDWGEDEPDDLDGAPVDDEPVQVDPAAQDPPPG"
     gene            complement(3457..4899)
                     /locus_tag="PKN91_03530"
     CDS             complement(3457..4899)
                     /locus_tag="PKN91_03530"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNS27176.1"
                     /translation="MRQVFTLMLCCALTATAVAQSGSDLPDMGNPVDAILTQGDEYRI
                     GLTVLRQLREQGQVLEDPEISEYIQALGTRIAAQATDEGQRFTFFVIRDTAINAFALP
                     GGFIGVNQGLLLATKNESELASVLAHEIAHVTQRHIARSIRAAGRQSLATTAMVIAGI
                     LLGAMGGGDAAQAAIAVAQGSAAQQQINYTRSNEHEADRIGISFLASAGFDPHAMPAF
                     FETMQRRSGLSRLDIPEFILTHPVTSTRIAESRDRAESYPSPKPNPEVSYQVARERAR
                     VLVAPDDAGLERYYAGELAAGRDGVAERYGAALADLGNGNAGDAVAALSTLRSAHEGN
                     PMIASSLGQAQMAAGATEAALATFAEALRLSPRNVPLTIRYAEALMRVDRAREAHVML
                     LDLFNNVAPTPEQIRLTALAASAAGDTGDAYFYMSEYHIANGDLPLSVQQLELALAAP
                     DLSGVQRQRFQARMDEVREAIDRGRKRGRS"
     gene            complement(4943..6361)
                     /locus_tag="PKN91_03535"
     CDS             complement(4943..6361)
                     /locus_tag="PKN91_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNS27177.1"
                     /translation="MKTRHIPRESRIFVLDTNVLMHDPTSIFRFEEHDIYLPMIVLEE
                     LDANKKGLSEASRNVRQVSRFLDELMRDATKEQIDAGLPLPSGYSGNHGKKPSSGRLY
                     FQTRQLSSALPEYLPGHGSDNAILAQVLALQALHTDARVTLVSKDINLRIKAAVVGVH
                     AEDYYSDKTIEDADVLYTGHAPLPADFWERHDRDMRSWKEGDRTWYTLTGPAVQEWQV
                     NEFVYQQDDQGIEAIVRRIEGDTATVELTRDYRNERHSVWGITARNREQNFALNLLMD
                     PEIDFVSILGPAGTGKTLLTLAAGLMQTLESNRFAEIIMTRVTIPLGEDIGFLPGTEE
                     EKMEPWMGALMDNLEVLTQSQEGGNWGRAATNDLLRNRIKIRSLNFMRGRTFLNRYII
                     LDEAQNLTPKQMKALVTRAGPGTKLVCLGNIAQIDTPYLTETTSGLTYVVNRFRGWQH
                     SGHITLVRGERSRLADHASEIL"
     gene            complement(6405..6863)
                     /locus_tag="PKN91_03540"
     CDS             complement(6405..6863)
                     /locus_tag="PKN91_03540"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNS27178.1"
                     /translation="MKVGARVPALRLPQSDGSEWRLSDYAGQKVVLYFYPKDMTSGCT
                     LEGQAFRDLHPAFRRAKTAVFGISRDSCASHAKFRAREAFPFELLADEDEKACRLFDV
                     IREKNMYGRKVMGIERSTFLIDASGRLAREWRKVKVKGHAEEVLEEAKHL"
     gene            complement(6860..7390)
                     /locus_tag="PKN91_03545"
     CDS             complement(6860..7390)
                     /locus_tag="PKN91_03545"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNS27179.1"
                     /translation="MKQHLAVSAVGTDRTGIVHELSRVITECGGSISESRMANLGSEF
                     AMLLLISGNWHSLAKIESELKKLGETAGLTINVKRTEPRGPRTDMVPYSVDVVCVDQT
                     GIVASLAGFFSSRSIDIAELATRVYSAAHTGATMFSVQMIVNVPTRIHLAHLREEFMD
                     FCDSLNLDAIMEPVKS"
     gene            7513..8397
                     /gene="dapA"
                     /locus_tag="PKN91_03550"
     CDS             7513..8397
                     /gene="dapA"
                     /locus_tag="PKN91_03550"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNS27180.1"
                     /translation="MFSGSLVAAITPMRHDDTIDFEAWSRLVDFHVAHGTSGVVVGGT
                     TGESATLTEAELVELVTRARERAAGRLQVIAGAGTSSTALTVERAGRLSRLGVDALLV
                     VTPAYNRPTQEGLYRHFEAVAQASIVPLILYNVPSRTAVDLLPPTVARLAALPRIAAI
                     KEAVNSMERIGQLVALAPPGFTILSGDDATARDAVARGARGVISVTANVAPRLVADMI
                     AAALAGDTRRAAELDGRLAGLHEALFVEPNPIPAKWVVSQLLGLAPPGIRLPLTPLEP
                     RHEATLRRAMMAAGLALD"
     gene            8408..8686
                     /locus_tag="PKN91_03555"
     CDS             8408..8686
                     /locus_tag="PKN91_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27181.1"
                     /translation="MRNVIVALLLGVVLAGCRTFGGGGSCNKPREYTNSESIPALKVP
                     PGMDVPNTRGALRIPELDTEERVRGPGDPCLDEPPPFAPPPATPQPQA"
     gene            8731..8821
                     /locus_tag="PKN91_03560"
     tRNA            8731..8821
                     /locus_tag="PKN91_03560"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8765..8767,aa:Ser,seq:gga)
     gene            8863..8960
                     /gene="ffs"
                     /locus_tag="PKN91_03565"
     ncRNA           8863..8960
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKN91_03565"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            8982..10553
                     /gene="dnaX"
                     /locus_tag="PKN91_03570"
     CDS             8982..10553
                     /gene="dnaX"
                     /locus_tag="PKN91_03570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNS27182.1"
                     /translation="MSYTALARKWRPKRFAEMVGQQHVLQALANALAGGRVHHAFLFT
                     GTRGVGKTSVARILARCLNCETGVTAEPCGECSACRAIDEGRFIDLIEVDAASRAKVD
                     ETRELLENVQYAPSAGRYKVYLIDEVHMLSQHSFNALLKTLEEPPPHVKFLLATTDPQ
                     RLPVTVLSRCLQFSLKRLPVNLIAARMAEILEAEGIGFEAPALTLLAHAADGSMRDGL
                     SLLDQLIAFGGGSVTTTAAREMLGTIDRERVAAILDALATGDARRVIDAAHALEEWAP
                     DYGAVLDELAGVLVQVALRQAVPDYDLSELPDAALVERLAATVPAEDVQLYYQTAIIG
                     RRDLPLAPDPRTGFDMVLLRMLAFRLPGDGAVAKPGAQPAPAVRTAVAPNTRGAAPVG
                     DDALSAAGWPQLVASLDLSGLARQFAAHCAFLGRQGRLVRLGLDARHAAMRTPTQEDR
                     LARALSRRLGETVRLEIEVVEGELPTPAADRERESAADLEAARATLAGDPAVRALQEK
                     FGATLHPESVRPTGK"
     gene            10565..10888
                     /locus_tag="PKN91_03575"
     CDS             10565..10888
                     /locus_tag="PKN91_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008844819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNS27183.1"
                     /translation="MARNFGNMMKQAQALQANMQKAQAEIANLEVTGESGGGMVKVTM
                     NGKHDVKRVQIEPAVIGEDREMLEDLIAAAFNDAVKKAEQRSAEKMSGLMAGLQLPPG
                     MKLPF"
     gene            10905..11501
                     /gene="recR"
                     /locus_tag="PKN91_03580"
     CDS             10905..11501
                     /gene="recR"
                     /locus_tag="PKN91_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011104642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNS27184.1"
                     /translation="MKHSPALAGLIDALRTLPGVGPKSAQRMAFHLLQDGRAGANALA
                     ASLATALATVRRCSRCRMLTEAELCSICESRRDADLLCVVESPADVVAIESSGSFRGR
                     YFVLMGHLSPLDGIGPEQIGIRELEALLAQPGLREVILATNPTVEGEATAHFLSEVAR
                     RHGIAATRIAHGVPIGGELEYVDGGTLAHALAGRQSIS"
     gene            11519..12226
                     /locus_tag="PKN91_03585"
     CDS             11519..12226
                     /locus_tag="PKN91_03585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS27185.1"
                     /translation="MDEPHPLDNPMWQALTTRQRSFAIGEGNVRRYAPEVAPFAAVRD
                     PGVDIDAELAAIVPVGEVVILVGVSPAVSRAWRVLDSGVVIQMTCAGAPPPVPAGARW
                     AELGAQDRPAMLALTALVYPEYFRERTAELGRYIGIHREGGLAAMAGERLATDSAVEI
                     SAVCTHPRCTGRGYATLLVALLARDIAARGALPFLHVSEHNTRAIDLYQRMGFRERAR
                     LGLWKLRREAGEGRAAR"
     gene            complement(12238..13173)
                     /gene="rsgA"
                     /locus_tag="PKN91_03590"
     CDS             complement(12238..13173)
                     /gene="rsgA"
                     /locus_tag="PKN91_03590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNS27186.1"
                     /translation="MTTAAKEFDAEVIATFGRHALVRDRAGAEIEARPHGRRLVIACG
                     DQVRCVRDAHHQEVHVTEVLPRRTALWRSNLRGGAEIIVANISHLVAVIAPRPEPDFF
                     VVDRYFAAAASSGIASVLVANKADLGLDQSLIDELAAYEAIGATTVTCSAATGEGIDA
                     LKGLLKAATAVLVGQSGVGKSSIVNALVPGAAVTTSDLVRQTEEGKHTTTTARLYDLP
                     GGGRLIDSPGVRDFSPAIDHLEPGTLGYTDVAWLAPSCRFLDCRHMREPGCAVIAAVQ
                     EGKMHARRHESYRRMRRLYDRLAAARGPARRPRQP"
     gene            13232..13789
                     /gene="orn"
                     /locus_tag="PKN91_03595"
     CDS             13232..13789
                     /gene="orn"
                     /locus_tag="PKN91_03595"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HNS27187.1"
                     /translation="MTSPDHLIWIDLEMTGLVPETDAIIEIATVVTNGALEVVAEGPV
                     IAIHQPDEVLARMDEWNQRQHGASGLVARVRASTVGTAEAERATLAFLAQHVPAGASP
                     MCGNSICQDRRFLARLMPELERHFHYRNLDVSTLKELARRWAPRVLDGVVKEGTHLAL
                     ADIRDSIAELRHYRRHLLAPEFRGE"
     gene            13782..14747
                     /locus_tag="PKN91_03600"
     CDS             13782..14747
                     /locus_tag="PKN91_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNS27188.1"
                     /translation="MSDPLPDFAAVVAAAGRLQGRVRRTPVMHADRFDERAGRRVVFK
                     CENLQTGGAFKYRGALNALLSLPPGAGVTHVATHSSGNHGTALALAARSLGLACTVVV
                     PAGASPPKVAAIEAAGGRVVRCEAGLAAREAALAALLAREPAHVVHPYDDADVIAGQG
                     TAALEMLAEPLDFDTVVAPVGGGGLIGGTALAARGLLDECAVIAAEPAAADDAWRSFT
                     SGKRVGASAPDTIADGLRASIGVRNFELLRRHVDEVVTVSEAEIVRAMRIVLEDLKLL
                     IEPSSAVAVAALLSPRATRLGERVGVILSGGNVDLDQCPFLAGRT"
     gene            14744..15133
                     /locus_tag="PKN91_03605"
     CDS             14744..15133
                     /locus_tag="PKN91_03605"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNS27189.1"
                     /translation="MTTHSGGCHCGAVRFEVDAPAAIEALECNCSMCRATGFLHLIVP
                     RSRLRLLTDPGALRTYTFNTGVAQHLFCATCGIKSFYVPRSNPDGYSVNVRCLDPRTI
                     ASLTVRQFDDHDREAAEARIRHFSQGP"
     gene            15189..15548
                     /locus_tag="PKN91_03610"
     CDS             15189..15548
                     /locus_tag="PKN91_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HNS27190.1"
                     /translation="MKILANAMVGLVALLHVYILVLEMFLWDKPAGQRAFRMTAEQAS
                     ATKVLAANQGLYNGFLAAGLLWGLALGAGGTPVKLFFLGCVFVAGVYGGVTACRKILY
                     IQALPAALAFALVWLTG"
     gene            complement(15538..17892)
                     /gene="ppsA"
                     /locus_tag="PKN91_03615"
     CDS             complement(15538..17892)
                     /gene="ppsA"
                     /locus_tag="PKN91_03615"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNS27191.1"
                     /translation="MTTYVLPFEKLKKNDVETVGGKNASIGEMIGALARLGVQVPGGF
                     ATTADAYREFLQQGGLDARIRGALDGLDVDDVRKLATTGAQIREWILSTPFPPALEAA
                     VLEAFRAMGAGREIAVAVRSSATAEDLPEASFAGQQETFLNVRGEAQVLKAMHEVFAS
                     LFNDRAIAYRVHQGFDHALVALSAGVQHMVRSDLGASGVMFTLDTDSGFREVVFITAS
                     YGLGETVVQGAVNPDEFYVYKPALRAGRPAILRRNLGGKAIKMVYGDAGSGERVKTID
                     VPRADRQRFSLDDADLVSLARQALAIEEHYGQPMDIEWGKDGEDGQIYILQARPETVQ
                     SRAGRAIQRYSLKQRSKVLATGRSIGQRIGAGPARIIRDVAEMARVQTGDVLVADMTD
                     PDWEPVMKRAAAIVTNRGGRTCHAAIIARELGIPAVVGCGDATSKIKEGAEVTVSCAE
                     GDSGYVYEGLLDFERRQIELDTLPPIPVRIMMNVGNPDRAFDFAGIPHRGVGLARLEF
                     IINRMIGVHPRALLEFDTLSGELKEQIRGQMAGYTDPVSFFVEKLAEGISQIAAAFAP
                     EPVIVRLSDFKSNEYANLIGGRQYEPSEENPMLGFRGASRYIDTTFRPCFELECQALK
                     RVRNDMGLTNVQVMVPFVRTVEEARQVTELLAANHLARGENGLKLIMMCELPTNALLA
                     ERYLEYFDGMSIGSNDMTQLTLGLDRDSGIVAKLFDERDDAVKALLEMAISACRKQGK
                     YIGICGQGPSDHPDFARWLLERGIDSISLNPDTVVETWMFLANQ"
     gene            17961..18782
                     /locus_tag="PKN91_03620"
     CDS             17961..18782
                     /locus_tag="PKN91_03620"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HNS27192.1"
                     /translation="MNRRTVFFVSDQTGVTAETMGHSLLTQFDGLEFRPVTLPFVSSI
                     DKAEDAVRKINLAAEESGVRPIIFSTLVQDDVRSVVMRAHGLFLDFFDAFVGPLEKEL
                     ALRSTHRTGRAHGMTDPTAYTTRINATNFALRNDDGSGRDYDAADVIVVGVSRVGKTP
                     TCLYMALQYGIFVANYPFNEDDLEGGRLPQAIAPFRNKLYGLTIHPERLQQLRHERRP
                     DGRYASQQQVQYELRAAEALFARYSIPCLDTTECSIEEIASHILETSGIERRVRP"
     gene            18848..19231
                     /locus_tag="PKN91_03625"
     CDS             18848..19231
                     /locus_tag="PKN91_03625"
                     /inference="COORDINATES: protein motif:HMM:NF018552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HNS27193.1"
                     /translation="MQTDALATVSWRARPRSFVALMALYESNYVRLGWLAGDPGRLCA
                     TASSCIEGDCELRLTVCERARYTTTIELTYAPIAMPDLRVRVYHDARLAEAQCGGGMH
                     ERWTHNMLLNKWLEYCVDRGHRIGS"
     gene            19244..20299
                     /locus_tag="PKN91_03630"
     CDS             19244..20299
                     /locus_tag="PKN91_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27194.1"
                     /translation="MFSVKPLANLRFGKEPEKEAEEQRVVQLFRNRAELKKAYGSLQD
                     EVHRLKDRLKQQEGATARVQEMVEALEARLAAPDTGYPALVFYHLRGLWSFGRDLIAQ
                     LVADLARQQEEKERRQHLTESNRRQFARRQALDARLREAETAAAESLARHADAQRRLA
                     ALTRFWHYFQRRELGRRLQVLEADSRAAAAALADARDAHEALDSEQAAEFPGLSIEAR
                     RAINLAAIGYAELLCLRLARTQIVALAKDAAARREVIDDYGTREECERLMADILRAKA
                     VLQHRGQLSQELRVRTDRLRQLARYRNPADSMPTADSIGMAEGDVLDRGGQGANAARL
                     PNVLAEDTWDLFRVVLR"
     gene            complement(20280..23369)
                     /locus_tag="PKN91_03635"
     CDS             complement(20280..23369)
                     /locus_tag="PKN91_03635"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNS27195.1"
                     /translation="MIHTNFALRRPVTTIMVFAAVATIGAMAARLLPLEQFPDITFPF
                     MGVTIPYPGSTPEEIEDEITRPVEDALATLPGIREIRSKSESDQATFEIEFDWGTDVE
                     AAGFEVRTKLDAIRHDLPAAANRILMFRASSADQSVLTIRLSADSDLSSQYEVLERTL
                     KKPIERVDGVARVELAGVEPREVRVLVDAGRIAAHRIDVRELVQKLQRANFSVSAGLM
                     TDGTERFTVRPIGELRSLEELRKLVIGPDLRLQDVAEVELVTPELTTRRRLNGSPAVG
                     LDVFKTTQANVIDVADSVLEVLELAHDLPQLQGVTIYVIENQATSIRSSLADVGEAGL
                     IGAVLALIVLYLFLRHWPTTLIVSLAVPLSLLVTLAAMYFMDLSINVMTMMGMMLAIG
                     MLVDNAVVVTESVFRHRQLDPANPLGATLAGVREVGIATLAGTATCVVVFLPILFGER
                     NQLSIFLSHVAVPIVVAMSASLVIAQTIVPMLTSRFPPPPPIAHSSVFARVQDRYSAI
                     LRASLTHPRRPAAALGVLLALTIGLLVASGMFPDKLLRFDMFPQDAGRELYIDYRIGG
                     THPIDRVEAAVKTVESYLAEHREEFDIESVYSRYDASSANSILKLTSKEDMRIPASEV
                     MRRASADMPEIIIGKPTFNLEGAAATGGFSMRITGESTERLAVLAADVARALRGIEGL
                     TSIRSEARDGDDEVRISVDRNRAAALGVTPQDAAVSIAAALRGDRLREFRSADRELTL
                     RLAFRESDRQNIADLARLPLYLPSGQRIELGAIADFAIERGPRAIQRVNRLTSVTLGG
                     VIADDATLPVVRERVAKMMAAYQLPPGYSWQFGRGVQQSDDGTQAMIFNLLFAVAMIY
                     LVMAALFESTILPISIVTSILMAVIGVIWTLFLTGTTFTFMAIIGVQILIGVVVNIGI
                     VLVAHINDLRTAGTTRLEAIIQASRDRLRPILMTTLTASLALSPLAFGDAQLAVGLAG
                     PSYAPMARAIMGGLVFSAATSLFAVPVFYQWLDDGIVAMRRFMARTRAAGEVSAARP"
     gene            complement(23366..26719)
                     /locus_tag="PKN91_03640"
     CDS             complement(23366..26719)
                     /locus_tag="PKN91_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNS27196.1"
                     /translation="MRIVDLALRRRVTIAMATVAVALFGAVSLSRLKVNLLPDLSYPT
                     LTIRTELPGAAPLEVENLITRPIEEAAGTIRNVRSVRSVSRSGQADVVLEFAWGTDMD
                     FAGIEVRERLDLLQLPTDAERPLLLRFDPSTEPVLRLAFVDLAEGSAASSERLKTLRR
                     YADDRLKPELEATEGSAAVKVSGGFEDEIRILVDQERLAQLKLDVASVAARIGAENVN
                     LSGGRLEQGTQRFLVRTLNEFQSLEEMSSAVIATVEGRPVYLRDIAQVEHGYKDRTAI
                     TRLDGRECVELAVYKEGDANTVLLAETVRARLATLQKSLPPETKLQTVYDQSQFIANS
                     VSEVSDAAIWGGLLSVIVLYLFLRDARATLVAGIVIPVTVIGIFVLMYGFDLTLNIMS
                     LGGIALSVGMLIDNSVVILEAISRKREAGMPAVQAAREGTAEVAMAVTASTLTTVAVF
                     FPMVFVSGIAGQLFRDQALTVTFAQLLSLLASLTLVPMMAAWRANDGERRVPRDELRA
                     ELAERGAIFAGRLREIVRALRPGEFGRAPAARGLRRIFAWLAWPLRLLLVTLIRLARA
                     LAGAVGALCGLVLAPAMHVTQRGFAALERLYPASLEWSLRHRSAVLGVAISLLAITGL
                     ILVRLPTELIPQLAQGEFNVKLRLPAGSPLDSTDRVLRAALSAAVDLPGLDHAYAVAG
                     TGNRLDANPVDSGENIGEMSIRLRQPAGPREEAAAMAQLRSALDALPGVQYEFSRPAL
                     FTLSTPLEVVLSGYELERLQSAAAAVHARMAASGAFTDLRSSIEGGHPEIQILFDQDR
                     ASRLGLEVRTIADSVVSSLRGDVATRYQLRDRKIDVLVRSVDTRSASIEEVRNLIVNP
                     GAEKPVTLAAVADVRLAIGPAEIRRAGQERVALLSAAPAQGNLGAATAIANEILATTP
                     LPAGITYGVTGQSEEMQQSFRSLLMAFALAVFLVYLVMASQFESLLHPFVILFTIPMG
                     LIGAVWGLAVTGTTINAVAFIGLILLAGIVVNNAIVLLDAVNQARARGLTRHDALVLA
                     GRTRLRPILITSVSTILGLLPMAIGLGEGAEVRRPMAVTVIGGMLIATVLTLIIIPVL
                     YTLLDRRRLPQDAGAAAPVPAPVAAGARP"
     gene            complement(26738..>27603)
                     /locus_tag="PKN91_03645"
     CDS             complement(26738..>27603)
                     /locus_tag="PKN91_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNS27197.1"
                     /translation="VKEGQLLAVLDGRQLRLEAAQARAQFAKAERDYRRQVELHDKGL
                     VSAGAFEGLKFDLENLRATYELAELMLSYTEIRAPFAGLVASRFVKVGQNVPQGTKTF
                     RVTDPTPLKASVYVPERELAHLAPGQSAVALVDALGSRSFPARVTLVSPSIDPATATF
                     KVTLELDDQDGVLKPGMFARVGIVFERRDAALQVPRVALIEADGTSSVFVVEDGKAVQ
                     RPVTTGLVDGGNIEVTTGVADGDSVVVVGHTGLKDGNAVRVVSLETRPTSALRAAAAG
                     KVAAGAQTANR"
BASE COUNT         4315 a         9179 c         9616 g         4493 t
ORIGIN      
        1 acgcgtcgcg ctggcgcgcg ccgtggtgat ggatccggaa ctgctgatct acgacgagcc
       61 gttcgtcggc ctcgacccga tttcgatggg cgtcatcgtg cggctcatca ggcagatgaa
      121 ccgcgtgctc ggcatcacca gcatcgtcgt atcgcacgat gtgcacgaac tcgccgccat
      181 tgccgacgag agttacctgc tgtcggaagg gcgctgtgtc gcctccggca gccccgaaga
      241 gctgcgcgtc agcgattcgg agatcgtacg acagttcctc gatggcattg ccgacgggcc
      301 ggtcccgttc cactttcccg cgccgggcta ccgcgaacag ctgttcggcg tgaacggcgc
      361 gcgaaggagc tcgcgatgaa ccgcgcagtc gcccgagacg gcatggtgac cgggatgctg
      421 cgccgcattg gcgcggtcgg cgtgttcttc ggcgcgctgc tggcgcagtg cgtgccggca
      481 ctcgcgcgcc cgcgcctgat cgttcaccag atctacaacg cgggcgcccg ctcgctgatc
      541 atcgtcatga tgtgcggcgt gttcgtcggc atggtgctcg gcctgcaagg cttcgacctg
      601 ctgcagcgtt tcggttccga ggatgcgctc ggcgttgccg ccgcgctcag cctgctgaag
      661 gagctgggac cggtggtcac ggcactgctg ttcgcaggtc gcgcgggtac ctcgctcact
      721 tccgagatcg gcctgatgcg cgccaccgac cagctgacgg cgatgcagat gatggccgtg
      781 gatccgatgc agcggatcat cgcgccgcgc tttctcggcg gcgtcatcgc gatgccgctg
      841 ctggtggcga tcttcaacgt ggtgggcctg ttcggcgccc agttggtcgg cgtgcagttg
      901 atgagcgtcg atcctggcgc gttctggtcg cagatgcagg gtgccgtcga gttgaacgat
      961 gttcaggaag gattcatcaa gagcctggta ttcggtacgg catgcagcct ggtcgctgtg
     1021 tacgagggct accattccga accgaccgcc gcgggcgtcg ggctcgcaac gacgcgcacc
     1081 gtcgtcacat cgtcggtgct cacgctgctg ctcgattaca tcctgaccgc agcattcctc
     1141 tgaagggttc gatcctatgc gtcccaatcg tgcacttgaa atcggtaccg ggctgttcgt
     1201 gctgctcgga ttcgcggcac tgctgttcct cacgacccag ctgccgggca gtggcgccag
     1261 gctcggcggc ccccagggct atgtcgtgac cgcacgattc gacaatatcg gcgacctgaa
     1321 gccgggctcg cccgtcacgc tcgccggtgt gcgcatcggc cgcgttgccg gcattgattt
     1381 cgacccgacc gacttcaagg ccgtggtgac cctcgagatc gaggaccgct acagccagat
     1441 tccggacgac agcgacgcga gcatccagac tgcgggcctg ctcggtggca agtacgtcgg
     1501 cattggtccg ggaggctccg aaacctacct gcatgcgggc agcacgatcg agttcacgca
     1561 gtcggcgatc gtgctcgaga gcctcgtcaa caagttcttc gcgaacttcg cgagcggacg
     1621 cgactcaaaa gaagcgccgc aggacgcgca gggtgaaacg ccatgaccat gaccatgacc
     1681 atgacgatga cacgcaagtc cattcccatg ctacttgccg cgatgctgct cgctgcgccg
     1741 gtactggccg ccgattccgt ggatgcgagc ggtcctggcg agctgatcca gaccgcggcg
     1801 cagtcgatgc tcacggcgct cgatgcggat cgcgaggcct atcggcagga tccgaagaag
     1861 gtcgagcgtc tggtggagga agtgctgctg ccacacttcg atgcacagta cgcggcgcgc
     1921 ctggtgctgg cgaagcactg gcgtacggcg acgccggagc agcgcgagcg cttcatcaag
     1981 ggcttctaca attcgctgct gcgcaattac ggcacagcgc tcgccgagtt caccggtgac
     2041 cggctcaagg tcttcccggc gcagatcgcg ccggacggcg aacgtgccac cgtgcgcacc
     2101 gaggtgaagc gtagcaacgg cgaacgcgtg cccgtgaatt acacgctgcg tcgcaccgag
     2161 ggcgcctgga aggcatggga tgtcaccatc gagggcatca gctacgtgaa gagtttccgc
     2221 gaggatttcg gcagcgagat cgacgcgaaa ggtctggatg ccgtgatcga gcgcctcgag
     2281 cgcggcgatt cgccggtgaa gaagtccgcg tgatcgagct cgtggcgatc gcgccgggcc
     2341 gcctcgcgct gcgcggcggg ctcgacttcg gcagcgccgt gatcgcgcac caggccggac
     2401 tcgccttctt ctcgcagggc gacacgcgcg agtggaccgt ggactgcagc ggactcaccg
     2461 acgcgaacag cgcggcgctc gcggtgctgc tcgactggct ggctgcgatg caccgcagcg
     2521 gcggcaagct gcgttacgag ggactgcccg agcgagtgct cgcgatcgca gagatcagcg
     2581 aagtcgacga attgctcgca gcgggagtct gaccgctcaa cccggaggcg ggtcctgcgc
     2641 cgcgggatcg acctggactg gctcgtcgtc gaccggcgcg ccgtccaggt catcgggctc
     2701 gtcttcgccc cagtcgatgc cttcgtctgc cggcacgtcg atcacgttgc cgtcctggat
     2761 gacatattcg cgctgctgga aatacgcgtt gcggatgaac gcataggggt cgtgcacgtc
     2821 cttgagcgcc tccgttgccg gcaggagcct ggcgcgccgt tcgagcaggc gcaggccgtc
     2881 gagaccccat tgccagcgcc agtcgtcgac aaagtggacc gggtcggcaa atcggtcggc
     2941 aatccgcccg gcgccatcgc ggaacgagct cggtccgagc agcggaatga tgatgtacgg
     3001 cccactcgga acgccccact tgccgagcgt ctggccgaaa tcctcgtcgt tgcgatcgag
     3061 accggcggcg gtggccggat cgaggatgcc gccgatgccg gcgatcgtgt tcagcaggaa
     3121 gcgttcgagg tcgcgcaggc cggtgagcgg cttgccctgc aggaactggt tgatgatcgt
     3181 gctcggatag gtggcgttgc tccagaaatt ggtgacgccg gtctgcacga gctgtggcgt
     3241 gatcttgcga taggccgtgg ccacgggctt cccgatcgcg cggtcgaggc ggtcattgac
     3301 ggtggagatc gtccggttga tcttctcgag cgggtcgcga gggtccggct tcagtgtctg
     3361 ggtcgtgcag ccggtcagca cgagcgtggc tacggccacg aggatccaat ggggcatcgg
     3421 cgcatgatac agggcgccgc gccctgcgct aagggctcag cttctgcctc gcttgcgtcc
     3481 gcgatcgatc gcttcacgca cttcatccat gcgggcctgg aaacgctggc gctgcacgcc
     3541 ggacagatcg ggagcggcga gggcgagttc gagctgctgc accgacagcg gcaggtcgcc
     3601 gttcgcgatg tggtactcgc tcatgtagaa gtacgcgtcg ccggtgtcgc ccgcggcgct
     3661 cgcggcgagc gcagtgaggc ggatctgttc gggcgtaggc gccacgttgt tgaacaggtc
     3721 gagcagcatc acatgcgcct cgcgcgcgcg gtcgacccgc atcagggcct cggcatagcg
     3781 gatcgtgagc ggcacgttgc gtggcgagag ccgcagcgcc tcggcgaagg tcgcgagcgc
     3841 cgcctcggtc gcgccggccg ccatctgcgc ctggccgagc gacgacgcga tcatcggatt
     3901 gccttcgtgc gccgacctca gggtcgagag cgcggctacg gcatcgcccg cgttgccgtt
     3961 gcccaggtcg gcgagtgcgg cgccgtagcg ttcggcgacc ccgtcgcgac ccgcggccag
     4021 ctcgcccgcg tagtagcgtt cgagcccggc gtcgtcgggc gcgaccagca cgcgtgcccg
     4081 ctcgcgggcc acctggtaac tgacctcggg attcggcttc ggactggggt agctctccgc
     4141 gcgatcgcgc gattcggcaa tgcgcgtcga ggtgaccggg tgggtcagga tgaattcggg
     4201 gatgtcgagg cgggacagtc cggagcgccg ctgcatcgtc tcgaagaacg ccggcatcgc
     4261 gtgcgggtcg aatcccgcgg acgcgaggaa ggagatgccg atgcgatcgg cctcgtgctc
     4321 gttcgagcgt gtgtagttga tctgctgctg cgcggccgag ccctgcgcca ccgcgatcgc
     4381 cgcctgcgcc gcatcgccac cgcccatcgc gccgagcagg atgcccgcga tcaccatcgc
     4441 cgtggtggcg aggctctggc ggcccgcggc gcggatgctg cgggcgatgt gtcgctgcgt
     4501 gacgtgcgcg atttcgtgcg cgagcacgct cgcgagctcc gattcattct tcgtcgcgag
     4561 cagcaggccc tggttgacgc cgatgaatcc gccaggcagc gcgaatgcgt tgatggccgt
     4621 gtcgcgtatg acgaagaacg tgaagcgctg gccctcatcg gtggcctgcg ccgcgatgcg
     4681 cgtgccgagc gcctggatgt attcggagat ctccgggtcc tcgagcacct ggccctgttc
     4741 ccgcagctgc cgcaataccg tgaggccgat gcggtactcg tcgccctggg tgaggatcgc
     4801 gtcgaccggg ttgcccatgt cgggcaggtc gctgccggac tgggcgaccg cggtcgccgt
     4861 cagcgcgcag cacagcatca gggtgaaaac ctgcctcatt cgctattcga ctggcgccgg
     4921 ccctgaaggt tcaggcggcc gctcagagga tctccgaggc gtggtcggcg agccgcgatc
     4981 gctcgccgcg caccagcgtg atgtgccccg agtgctgcca gccgcggaac cggttcacga
     5041 cgtaagtgag gcccgatgtc gtctcggtga gatagggcgt gtcgatctgc gcgatgttgc
     5101 cgaggcacac gagcttggtc cccgggcccg cgcgcgtgac cagcgccttc atctgcttgg
     5161 gcgtcaggtt ctgcgcctcg tcgaggatga tgtagcggtt gaggaaggta cggccgcgca
     5221 tgaagttgag cgaacggatc ttgatgcggt tgcgcagcag gtcgttggtc gctgcgcgac
     5281 cccagttgcc gccttcctgg ctctgggtca gtacttccag gttgtccatc agcgcaccca
     5341 tccacggctc catcttttcc tcttcggtgc cgggcaggaa gccgatgtcc tcgccgagcg
     5401 ggatcgtcac gcgcgtcatg atgatctcgg cgaagcggtt gctctcgagc gtctgcatca
     5461 ggcccgcggc gagcgtcagc agagtcttgc cggtgcccgc cgggccgagg atcgacacga
     5521 agtcgatctc ggggtccatc aacagattca gcgcgaaatt ctgttcgcga ttgcgcgccg
     5581 tgatgcccca cacgctgtgg cgttcgttgc ggtagtcgcg cgtcaattcg acggtcgcgg
     5641 tgtcgccttc gatcctgcgt acgatcgcct cgatgccctg gtcgtcctgc tggtagacga
     5701 actcgttgac ctgccattcc tgcacggccg gcccggtcag cgtgtaccag gtccggtcac
     5761 cctccttcca cgagcgcatg tcacggtcgt gtcgctccca gaaatcggcc ggcagtggcg
     5821 catggccggt gtacaggaca tcggcgtcct cgatcgtctt gtcgctgtag tagtcctcgg
     5881 cgtgcacacc gaccaccgcg gccttgatgc gcaggttgat gtccttcgag acgagcgtga
     5941 cgcgcgcatc cgtgtgcagc gcctgcagcg cgagcacctg ggcgaggatc gcgttgtcgc
     6001 tgccgtggcc gggcaggtac tcgggcagcg ccgaggagag ctggcgcgtc tggaagtaca
     6061 gacgcccgga agagggcttc ttgccgtggt tgccggaata gcccgacggc agcggcaggc
     6121 cggcgtcgat ctgctccttg gtggcatcac gcatcagctc gtcgaggaag cggctcacct
     6181 ggcgcacgtt gcgcgaggcc tccgagagac ctttcttgtt cgcgtcgagt tcctcgagca
     6241 cgatcatcgg caggtagatg tcgtgttcct cgaagcggaa gatcgaggtc ggatcgtgca
     6301 tcagcacatt cgtgtcgagc acgaagatgc ggctttcgcg ggggatgtgg cgggttttca
     6361 agttttcggg atgggtatga ctgctgcttc cggcgcctgc gccgtcagag atgcttcgcc
     6421 tcttcaagga cttcctccgc gtggcctttg actttcacct tgcgccattc gcgcgccaac
     6481 ctgcctgatg cgtcgatcag gaaggtgctg cgttcgatgc ccatgacttt ccggccatac
     6541 atgttcttct ccctgatgac gtcgaacagc cggcaggctt tttcgtcttc gtcggcgagc
     6601 agctcgaacg ggaaggcttc cctggcccgg aacttcgcat gcgaggcgca gctgtcgcgg
     6661 gagatgccga acacggcggt cttggcgcgc cggaacgccg gatgcaggtc gcggaaggcc
     6721 tgaccttcga gcgtgcagcc ggaagtcatg tccttcggat agaagtacag caccaccttc
     6781 tggccggcgt agtccgacag gcgccactcg ctgccgtcgc tctgcggcag acgcagcgcc
     6841 gggacccggg cgccgacttt cacgacttga cgggttccat gatcgcgtcg aggttgagcg
     6901 aatcgcagaa gtccatgaat tcctcccgca ggtgcgcgag gtggatgcgg gtgggcacgt
     6961 tgacgatcat ctgcaccgag aacatggtgg caccggtgtg cgcggccgag tagacgcgtg
     7021 tcgccagctc ggcgatgtcg atcgagcggc tcgagaagaa gcccgccagg ctcgcgacaa
     7081 tgccggtctg gtcgacgcac accacgtcga cggagtaggg caccatgtcg gtgcgtgggc
     7141 cgcgcggctc ggtgcgcttg acattgatgg tgagcccggc ggtttccccg agcttcttca
     7201 gttcgctctc gatcttggcg agcgagtgcc agttgccgga aatcagcagc agcatcgcga
     7261 actcggaacc gaggttcgcc atgcggcttt cactgatgct gccgccacac tcggtgatca
     7321 cgcgcgagag ctcgtggacg atgccggtac gatcggtgcc gactgccgag acggcgaggt
     7381 gttgcttcac ggggagagtg taccgggcgg cgggcgcaat gcgaaacggt ctgccagccg
     7441 cttgcctcgc gccagtatgg cccttaccat cgtccgactc ccttccacgc ccgccaagtt
     7501 tctggacccc ggatgttctc aggcagtctc gtcgccgcga tcacgccgat gcggcacgac
     7561 gacacgatcg atttcgaggc gtggtcccgg ctggtcgatt tccacgtcgc tcacggcacg
     7621 agcggtgtcg tggtcggcgg cacgacgggc gagtcggcca cgctgaccga agccgagctc
     7681 gtcgagctgg tgacacgggc gcgcgagcgt gcggcgggac gcctgcaggt catcgcgggt
     7741 gccggcacga gcagcaccgc cctgaccgtc gagcgcgccg ggcgtctctc gcgcctcgga
     7801 gtcgatgccc tgctcgtcgt cacgccggcc tataaccggc ccacccagga agggctgtac
     7861 cgccacttcg aggccgttgc gcaggccagt atcgtgccgc tgatcctcta caacgtgccc
     7921 tcgcgtaccg cggtcgacct gctgccgccg accgtggcgc ggctggccgc gctgccgcgc
     7981 atcgccgcga tcaaggaagc cgtcaattcg atggagcgca tcgggcagct ggtcgcactc
     8041 gccccgcccg ggttcacgat cctgagcggc gacgatgcca cggcgcgcga cgcggtcgcg
     8101 cgcggggcgc gcggggtcat ctcggtcacc gccaatgtgg ccccgcgcct ggtcgcggac
     8161 atgatcgcgg ctgcgctcgc gggcgacacc cgtcgcgccg cggaactcga tgggcggctt
     8221 gcgggtcttc acgaagcgct gttcgtcgag ccgaacccga tccccgccaa gtgggtcgtc
     8281 tcgcagctcc tcggacttgc cccgcccggc atccgcctgc cgctgacgcc gctcgagccg
     8341 cggcacgaag cgaccctgcg ccgtgcgatg atggcggcgg gcctggccct cgactgaagg
     8401 aaactgcatg agaaacgtga tcgtcgcgct gctgctgggg gtcgtcctcg ccggctgccg
     8461 gacttttggc ggcggcggga gctgcaacaa gccgcgcgag tacacgaatt ccgagagcat
     8521 ccccgcgctg aaggttccgc ccggcatgga tgtgccgaat acgcgcgggg cactgcgcat
     8581 acccgagctc gacacggaag agcgtgtgcg cgggcccggg gacccttgcc tcgacgaacc
     8641 gccgcccttt gcgccaccgc cagccacccc gcaaccccag gcctgacaag cctgatatga
     8701 tgggcggatc ctgtgtaatt cactgattcc ggagaggtgg ccgagcggtt gaaggcactt
     8761 cgttggaagc gaagcaacat cccaaaggtg ttcgagggtt cgaatccctc cctctccgcc
     8821 aacatcggca ctgccagtca gaggtcagcc catggcggct cgcattggtt cctccgcgac
     8881 gaccagccgc aaaccccgcc aggcccggaa gggagcaacg gtagcggcga tgtcgggcgc
     8941 cggagatcgg gctggtgcgg gtcgcctcca ttcctgagcc catgagctat accgcactcg
     9001 cgcgcaaatg gcggccgaaa cgcttcgccg aaatggtggg gcagcagcac gtcctgcagg
     9061 cgctggcgaa cgcgctcgcc ggcggtcgcg tccatcacgc gttcctgttc accgggaccc
     9121 ggggcgtcgg caagacgagc gtcgcgcgca tcctcgcccg ctgcctcaat tgcgagaccg
     9181 gtgtcacggc cgagccctgc ggcgagtgtt cggcgtgccg ggccatcgac gaaggccgct
     9241 tcatcgacct gatcgaggtc gacgcggcgt cgcgcgccaa ggtcgacgag acccgcgagc
     9301 tgctcgagaa cgtgcagtac gcgccatcgg ccgggcggta caaggtgtat ctgatcgacg
     9361 aagtgcacat gctgtcgcag cactcgttca acgcgctgct gaagacgctc gaggagccgc
     9421 cgccgcacgt caagttcctg ctggcgacga ccgacccgca gcgtctgccg gtcaccgtgc
     9481 tgtcgcgctg cctgcaattc agcctcaagc gcctgccggt gaacctgatc gcggcgcgca
     9541 tggcggagat cctcgaggcc gaaggcatcg gcttcgaggc ccccgcgctg acgctgctgg
     9601 cgcatgccgc cgacggcagc atgcgcgacg gcctgtcgtt gctcgaccag ctgatcgcct
     9661 tcggcggcgg atcggtgacg acgacggcgg cgcgggagat gctcggcacg atcgaccgcg
     9721 agcgggtcgc ggccatcctc gatgcgctcg cgacgggcga tgcgcgccgg gtgatcgatg
     9781 cggcgcacgc cctcgaggag tgggcgccgg actacggcgc ggtgctcgac gagctggcgg
     9841 gcgtgctcgt gcaggtcgcg ctgcgccagg ccgtgcccga ctacgacctg tcggaactgc
     9901 ccgatgcggc gctggtcgag cggctcgccg ccacggtgcc tgccgaagac gtgcagctgt
     9961 actaccagac ggcgatcatc ggtcgccgcg atctgccgct cgcgcccgat ccgcgcaccg
    10021 gcttcgacat ggtgctgctg cgcatgctcg catttcgcct gccaggcgac ggtgcggtcg
    10081 cgaagcccgg cgcgcaaccg gcgccggcgg tgcgcacggc agtggccccg aatacgcgcg
    10141 gtgcagcgcc ggtgggcgac gatgccttga gtgccgctgg ctggccacag ctggtggcga
    10201 gcctcgacct gtcgggactg gcgcgccagt tcgcggcgca ttgcgccttc ctcgggcggc
    10261 agggcaggct cgtgcggctc ggcctcgatg cacgccacgc cgcgatgcgc accccgacgc
    10321 aggaggatcg gctcgcgagg gcgctgtcgc gccggcttgg cgagaccgtg cgtctcgaga
    10381 tcgaagtggt cgagggcgag ctgccgacac cggccgcgga tcgcgaacgc gagtcggctg
    10441 ccgatctcga ggcggcgcgc gccacgctgg ccggcgatcc ggccgtgcgt gcgttacagg
    10501 aaaaatttgg tgccacactg catcctgaat cggtgcggcc cactggcaag tgagaggacg
    10561 aggaatggcc cggaatttcg gcaacatgat gaagcaggcg caggccctgc aggccaacat
    10621 gcagaaggcg caggcggaaa tcgccaacct ggaagtcacc ggtgagtccg gcggcggcat
    10681 ggtgaaggtc acgatgaacg gcaagcacga cgtgaagcgc gtgcagatcg agccggccgt
    10741 catcggcgag gaccgcgaga tgctggaaga cctcatcgcc gcggcattca acgacgcggt
    10801 caagaaggcc gaacagcgct ccgccgagaa gatgtccggg ctgatggcgg gcctgcagtt
    10861 gccgcccggc atgaaactgc cgttctgagc acccgcatcc cgccatgaag cattccccgg
    10921 cactcgccgg cctgatcgat gcgctgcgca cgcttcccgg cgtcgggccg aagagcgcgc
    10981 agcgcatggc gttccacctg ctgcaggatg gccgcgcggg cgcgaatgcg ctcgccgcat
    11041 cgctcgccac cgcgctcgcc acggtcaggc gctgcagccg ctgccgcatg ctgaccgaag
    11101 cggagctgtg cagcatctgc gaatcacgtc gtgatgccga cctgttgtgc gtggtcgagt
    11161 cgcctgcgga tgtcgtcgcg atcgagtctt ccgggagttt ccgcggccgc tatttcgtgc
    11221 tgatgggcca cctgtcgccg ctcgatggca tcgggcccga gcagatcggc atccgcgaac
    11281 tcgaggcgct gcttgcgcag cctggcctgc gcgaggtgat cctcgcgacc aatccgactg
    11341 tcgagggcga agccacggcg catttcctgt ccgaggtcgc gcgccggcac ggcatcgccg
    11401 cgacccgcat cgcccacggc gtgccgatcg gtggcgagct cgagtatgtc gacggtggca
    11461 cgctcgcgca tgcgctggcg ggcaggcaat cgatcagctg aggtccgcag caggcgccat
    11521 ggacgagccg cacccgctcg acaatccgat gtggcaggcg ctcaccacgc gccagcggtc
    11581 gttcgcgatc ggcgagggca acgtgcgccg ctatgcgccg gaagtcgcgc cgtttgcggc
    11641 cgtcagggac ccgggcgtcg atatcgacgc cgaactcgcg gcgatcgtgc cggtcgggga
    11701 ggtcgtgatt ctcgtcggcg tgtcacccgc cgtgtcgcgc gcatggcgcg tgctcgacag
    11761 cggggtcgtg atccagatga cctgtgccgg agcgccgccc cccgtgccgg cgggcgcgcg
    11821 gtgggcggaa ctcggcgcgc aggatcgacc ggcgatgctc gccctgaccg cactcgtgta
    11881 cccggagtat tttcgcgagc gcaccgctga actgggtcgc tacatcggca tccatcggga
    11941 aggcgggctc gcggccatgg cgggtgagcg tctggcgacc gacagcgccg tcgagatcag
    12001 tgccgtgtgc acccacccgc gctgcacggg caggggctat gcgacgctgc tggtggcgtt
    12061 gctcgcccgc gacatcgccg cgcggggagc gctgccattc ctgcatgtca gcgagcacaa
    12121 cacgcgggcg atcgatctct accagcgcat gggttttcgc gagcgcgcgc gccttggcct
    12181 ctggaagctg cggcgcgagg ccggggaagg ccgcgcggcg cgctgagcgg acaggcctca
    12241 gggctggcgc ggccgccgcg ccggaccgcg cgctgcggca agccgatcgt acagccgccg
    12301 catgcgccgg tagctctcgt ggcgacgtgc gtgcatcttg ccctcctgca cggcggcgat
    12361 gaccgcacaa cccggctcgc gcatgtgccg gcagtcgagg aaccggcacg agggcgcgag
    12421 ccaggccacg tccgtgtagc cgagcgtacc gggttccagg tgatcgatcg cgggcgagaa
    12481 gtcgcgcacg ccaggggagt cgatcagccg cccgccgccc ggcaggtcgt agagacgtgc
    12541 ggtcgtggtt gtatgctttc cctcctcggt ctggcgcacg aggtcggagg tggtgacggc
    12601 agcgccaggc accagcgcgt tgacgatcga agatttgccg acgccggact ggccgaccag
    12661 caccgcggtg gcagccttga gcagcccctt caacgcatcg atgccctcac cggtcgcggc
    12721 cgagcaggtc accgtcgtgg cgccgatcgc ctcgtaggcc gcgagttcgt cgatcagcga
    12781 ctggtcgagg ccgaggtccg ccttgttggc gaccagcacc gatgcgatgc cgctcgatgc
    12841 ggcggcggca aagtagcgat cgacgacgaa gaagtcgggt tccggccgcg gcgcgatcac
    12901 cgcgaccaga tgcgagatgt tggcgacgat gatctcggcg ccgccccgca ggttcgagcg
    12961 ccacagcgcg gtccggcgcg gcagcacctc ggtgacgtgc acttcctgat ggtgcgcgtc
    13021 gcgtacgcag cgcacctggt cgccgcaggc gatcacgagc cggcgcccgt gcgggcgcgc
    13081 ctcgatctcc gcgcccgcac ggtcgcgaac cagcgcatgc cggccgaagg tcgcgatgac
    13141 ctcggcgtcg aattcttttg cggctgtcgt cacgggcagc ggcagcgtag cctttgctag
    13201 tatccacgac aacattcccg gcgcattcac catgaccagc cccgatcacc tgatctggat
    13261 cgatctcgag atgaccggcc tcgtgccgga gaccgacgcc atcatcgaga ttgccaccgt
    13321 cgtcacgaat ggcgcgctcg aggtcgtggc ggaggggccg gtgatcgcca ttcaccagcc
    13381 cgacgaagtg ctcgcgcgca tggacgagtg gaaccagcgc cagcatggcg cgtcgggact
    13441 cgtcgcgcgc gtgcgcgcga gtaccgtggg caccgccgag gccgagcgtg cgaccctcgc
    13501 cttcctcgcg cagcacgttc cggcgggcgc gtcgccgatg tgcggcaaca gcatctgcca
    13561 ggaccggcgc ttcctcgccc gcctgatgcc tgagctcgag cggcacttcc actaccgcaa
    13621 cctcgatgtc agcacgctca aggaactcgc gcgccgctgg gcgccgcgcg tgctcgacgg
    13681 cgtggtgaag gagggcacgc atctcgcgct cgccgacatc cgcgattcga tcgccgagct
    13741 gcgccactac cgccgtcacc tgctggcgcc ggaattccgc ggtgagtgac ccgttgcccg
    13801 acttcgcggc cgtcgtggcc gcggcggggc gcctgcaggg ccgcgtgcgg cgcacgccgg
    13861 tgatgcacgc cgaccgcttc gacgagcgcg ccggccggcg agtggtgttc aagtgcgaga
    13921 atctgcagac gggcggggct ttcaaatatc gcggcgcact gaatgcgctg ctgtcgctgc
    13981 cgccgggcgc cggcgtcacc catgtcgcca cgcactcctc cggcaatcac ggcacggcgc
    14041 ttgcgcttgc ggcgcgttcg ctcgggctcg catgcacggt cgtggtcccc gcgggtgcgt
    14101 cacccccgaa ggtcgcggcc atcgaagccg ccggcggcag ggtggtccgc tgcgaggcgg
    14161 ggctcgccgc gcgcgaggcg gcgctcgcgg cactgctggc gcgcgaaccg gcgcatgtcg
    14221 tgcatcctta cgacgatgcg gacgtgatcg cggggcaggg taccgccgcg ctcgaaatgc
    14281 tggcggaacc cctggacttc gataccgtgg tcgcgccggt cgggggtggc gggctgatcg
    14341 gcggcacggc actggctgca cgcggtctgc tcgacgaatg cgcggtgatc gcggccgagc
    14401 cggcggccgc ggacgatgcc tggcgctcgt tcacgtcggg caagcgtgtc ggcgccagcg
    14461 ctcccgacac gattgccgac ggcctgcgtg catcgatcgg cgttcgcaac ttcgagctgc
    14521 ttcgccggca cgtcgatgag gtcgtcacgg tgtcggaggc cgagatcgtc agggcgatgc
    14581 gcatcgtgct cgaggatctg aaactgctga tcgagccgtc ctccgcggtg gcggtcgcgg
    14641 cgctgctgtc acctcgagcg acgcgcctcg gcgaacgtgt cggggtgatc ctgtcgggcg
    14701 gcaacgtcga cctcgaccag tgcccgttcc tggccgggcg cacatgacca cccactccgg
    14761 cggctgtcac tgcggggccg tgcgtttcga agtcgacgcg ccggccgcga tcgaggcgct
    14821 cgagtgcaat tgctccatgt gccgcgccac gggcttcctg cacctgatcg tgcccaggtc
    14881 gcgcctgcgg ctgctgacgg accccggtgc gctgcggacc tacacattca acaccggggt
    14941 cgcgcaacac ctgttctgcg cgacctgcgg catcaagtca ttctatgtgc cgcgctcgaa
    15001 cccggacggc tacagcgtga acgtgcgctg cctcgatccg cgcacgatcg cgagcctcac
    15061 ggtgcgtcag ttcgacgacc acgatcgcga ggccgccgag gcgcgcatcc gtcatttcag
    15121 ccagggaccg tagctgcgcc cgcgggcgat ctgctagcgt gggtgtggtc gaatcagggg
    15181 ggtgctgcat gaaaatactg gcgaacgcca tggtcggact cgtggcgctg ctgcacgtgt
    15241 atatcctcgt gctcgagatg ttcctgtggg acaagcccgc cgggcagcgc gcgttccgca
    15301 tgaccgcgga gcaggcgagc gccacgaagg tgctcgccgc gaaccagggg ctctacaacg
    15361 gcttcctggc cgcgggcctg ctgtgggggc ttgcgctcgg cgccggcggc acgccggtga
    15421 agctgttttt cctcggctgc gtgttcgtgg ccggcgtcta tggcggcgtc accgcctgcc
    15481 gcaagatcct gtatatccag gcgctgccgg cggcgctcgc ctttgcgctc gtctggctca
    15541 ctggttagcg aggaacatcc aggtctcgac gacggtgtcg gggttgagcg agatgctgtc
    15601 gatgccgcgc tcaagcagcc agcgcgcgaa gtcagggtga tcggacgggc cctggccgca
    15661 gatgccgatg tacttgccct gcttgcggca ggcggagatc gccatttcga gcagcgcctt
    15721 gaccgcatcg tcgcgctcgt cgaacagctt ggcgacgata ccggagtcgc gatcgagccc
    15781 cagagtcagc tgcgtcatgt cgttcgagcc gatcgacatg ccgtcgaagt actcgaggta
    15841 gcgttcggcg agcagtgcat tggtcggcag ctcgcacatc atgatcagct tgaggccgtt
    15901 ctcgccgcgc gcgaggtggt tggcggcgag cagttcagtg acctggcggg cttcctccac
    15961 ggtgcgcacg aacggcacca tgacctgcac gttcgtcaga cccatgtcgt tgcgcacgcg
    16021 cttcagcgcc tggcattcga gctcgaagca cggccggaac gtcgtgtcga tgtagcgcga
    16081 ggcgccgcgg aagccgagca tcgggttttc ttccgagggc tcgtactggc gcccgccgat
    16141 caggttcgcg tactcgttcg acttgaaatc ggagaggcgc acgatcaccg gctcgggcgc
    16201 gaacgccgcg gcgatctgcg agatgccttc ggccagcttc tcgacgaaga agctgaccgg
    16261 gtcggtgtag ccggccatct ggccgcggat ctgctccttc agctcgcccg acagcgtgtc
    16321 gaactcgagc agggcgcgcg gatgcacgcc gatcatgcgg ttgatgatga actcgaggcg
    16381 cgccagcccg acaccacggt gcgggatacc cgcgaagtcg aatgcgcggt cgggattgcc
    16441 gacgttcatc atgatcctga ccgggatcgg tggcagcgtg tcgagttcga tctggcggcg
    16501 ctcgaaatcg agcaggccct cgtacacata gccgctgtcg ccctcggcgc aggagacggt
    16561 gacttcggcc ccttccttga tcttcgaggt cgcatccccg cagcccacca cggcagggat
    16621 gccgagctcg cgcgcgatga tcgccgcgtg gcaggtgcgc ccgccgcggt tcgtgacgat
    16681 ggccgcagcc cgcttcatca ccggctccca gtcggggtcg gtcatgtcgg cgaccagcac
    16741 gtcgccggtc tgtacgcgcg ccatctccgc cacatcgcgg atgatgcgtg cgggtcccgc
    16801 gccgatgcgc tggccgatgc tgcgcccggt cgcgagcacc ttcgagcgct gcttcagcga
    16861 gtaacgctgg atcgcgcgcc cggcgcgact ctgcacggtc tcggggcggg cctgcaggat
    16921 gtagatctgc ccgtcctcgc cgtccttgcc ccactcgatg tccatcggct ggccgtagtg
    16981 ctcctcgatg gcgagcgcct gccgtgcgag cgacacaagg tcggcatcat ccagcgagaa
    17041 gcgctggcgg tcggcgcgcg gcacgtcgat cgtcttgacg cgctcgccgc tgccggcgtc
    17101 gccatacacc atcttgatcg ccttgccgcc gaggttgcgc cgcaggatcg ccggacgtcc
    17161 ggcacgcagc gcgggtttgt agacatagaa ttcgtcgggg ttcaccgccc cctgcacgac
    17221 cgtttcgccg aggccgtagg aggcggtgat gaacaccacc tcgcggaagc ccgagtccgt
    17281 gtcgagcgtg aacatcacgc cgctcgcgcc gaggtcgctg cgcaccatgt gctgcacacc
    17341 ggccgacagg gcgaccagcg catggtcgaa gccctggtgt acgcggtagg cgattgcgcg
    17401 gtcgttgaac agcgacgcga aaacctcgtg catcgccttc agcacctgcg cctcgccgcg
    17461 gacgttcagg aaggtttcct gctgaccggc gaacgacgcc tccggcaggt cttccgcagt
    17521 ggccgacgag cggacggcca ctgcaatttc gcggccggcg cccatcgcac gaaatgcctc
    17581 gagtacggcg gcttcgagcg cgggagggaa gggggtggag aggatccact cgcggatctg
    17641 cgcgccggtc gtcgcaagct tgcgcacgtc gtcgacatcg aggccgtcca gcgcgccccg
    17701 gatgcgcgcg tcgagcccgc cctgctgcag gaactcccgg taggcatcgg cggtggtcgc
    17761 gaaaccgccc ggcacctgca cgccgaggcg cgcgagcgcg ccgatcatct cgccgatcga
    17821 ggcattcttg ccaccgactg tttcgacgtc attcttcttg agcttctcga acggcagtac
    17881 gtacgtagtc aaatgtgggg ctccgggaat ccgctgcagc gcacttgcag ggtgggtcga
    17941 gcaatgaaac actcgaccgt gtgaatcgac gaacagtttt cttcgtttcc gatcagaccg
    18001 gggtgacagc agagaccatg ggtcacagcc tgctgaccca gttcgatggc ctcgagtttc
    18061 ggccggtgac tttgccattt gtgtcgagta ttgacaaggc agaggatgcc gtaaggaaga
    18121 tcaatctcgc ggccgaggaa agcggcgtca ggccgatcat cttctcgacg ctcgtgcagg
    18181 acgacgtgcg ctcggtcgta atgcgcgccc acggcctgtt cctcgatttc ttcgacgcat
    18241 tcgtcgggcc gctcgagaag gaactggctc tccgttccac ccatcggaca ggccgggcgc
    18301 acggcatgac cgatcccacc gcctacacga cccgcatcaa cgcaaccaat ttcgcactca
    18361 ggaacgacga cggcagcggg cgggattatg acgcagccga cgtgatcgtg gtcggagtgt
    18421 cgcgcgtcgg caagacgccg acctgcctgt acatggcgct gcagtacggt attttcgtcg
    18481 ccaactatcc gttcaacgag gacgatctcg agggcgggcg cctgccgcag gcgatcgcgc
    18541 cgttccggaa caagctgtac gggcttacga tacatcccga gcggctgcag cagctacgcc
    18601 atgagcgccg gcccgatggc cgctacgcct cgcagcagca ggtgcagtac gaactgcgcg
    18661 ctgccgaggc cctgttcgcg cgctactcga tcccttgtct cgacacgacc gagtgctcga
    18721 tcgaggagat cgccagccac atcctcgaga cctcgggcat cgagcggcgg gtccgtcctt
    18781 gagggccgtc acatcccggc accgcgctcc cttgcgaacc gcgatgcctg cgttatagtc
    18841 gtgacgaatg cagactgacg cgcttgctac ggtgtcctgg cgcgcacggc cgcgaagctt
    18901 cgttgcgctg atggcgcttt acgaaagcaa ttacgtgcgc ctcggctggc tggcgggtga
    18961 tcccggcagg ctctgcgcta ctgcatcctc gtgcatcgag ggcgactgcg agctgcggct
    19021 gaccgtctgc gagcgcgcac gctacaccac caccatcgaa ctgacctacg cgccgatcgc
    19081 catgcccgac ctgcgcgtgc gcgtctatca cgacgcgcgc ctcgccgaag cccagtgcgg
    19141 cggcggcatg cacgagcgct ggacacacaa catgctgctg aacaagtggt tggagtactg
    19201 cgtcgatcgc ggccatcgca tcggcagctg aggctcgcgc gcgatgttct ccgtcaaacc
    19261 gctcgccaac cttcgtttcg gcaaggagcc tgagaaggag gccgaggaac agcgtgtcgt
    19321 ccagctgttt cgcaaccgcg ccgaactgaa gaaggcctac ggctcgctgc aggacgaggt
    19381 acaccgtctc aaggaccgcc tgaagcagca ggagggcgcg accgcacgcg tgcaggagat
    19441 ggtcgaggcg ctcgaggcgc ggcttgccgc gcccgacacg ggctacccgg cgctcgtctt
    19501 ctatcacctg cgcggcctgt ggagcttcgg ccgcgacctg atcgcgcagc tggtcgccga
    19561 cctcgcgcgc cagcaggaag aaaaggaacg gcgccagcac ctgaccgaat cgaatcgccg
    19621 ccagttcgcg cggcgccagg cactcgacgc gcggctgcgc gaggcggaga ccgcggcggc
    19681 cgagtctctc gcgaggcacg cggacgccca gcgccgcctc gcggcgctca cccgtttctg
    19741 gcattacttc cagcgtcgcg aactcggccg gcgcctgcag gtgctcgagg cggattcgcg
    19801 cgccgcggcc gcggcactgg ccgatgcgcg tgatgcgcat gaagcgctcg acagcgaaca
    19861 ggccgcggaa ttcccgggcc tgtcgatcga ggcccggcgg gcgatcaacc ttgccgcgat
    19921 cggctatgcc gagctgctgt gcctgcggct cgcgcgtacg cagatcgtcg cgctggcgaa
    19981 agatgccgcc gcgcgtcgcg aggtgatcga cgactacggc acgcgtgagg agtgcgagcg
    20041 gctgatggcc gacatcctgc gcgccaaggc ggtgttgcag caccgcggcc agctgtcaca
    20101 ggaactgcgc gtgcggaccg accggctgcg ccagctcgcc aggtaccgca atcccgcgga
    20161 ttcgatgccg accgcggatt cgatcggcat ggccgagggc gacgtgctgg atcgcggcgg
    20221 gcagggcgcg aacgccgcgc gcctgccgaa cgtgctcgcc gaggatacct gggacctgtt
    20281 cagggtcgtg ctgcgctgac ttcgcccgcg gcacgcgtgc gcgccatgaa acggcgcatc
    20341 gccacgatgc cgtcgtcgag ccactggtag aacaccggca cggcgaagag cgaggtcgcc
    20401 gcgctgaaca cgaggcctcc catgatcgcg cgcgccatcg gcgcgtagga cggtccggca
    20461 agcccaaccg cgagctgcgc gtcgccgaag gcgagtggcg agagcgcgag cgaggccgtc
    20521 agggtcgtca tcaggatcgg gcgcagccgg tcgcggctcg cctggatgat cgcctccagc
    20581 cgcgtggtgc cggccgtgcg cagatcgttg atgtgcgcca ccagcacgat gccgatgttg
    20641 actaccacgc cgatcaggat ctgcacgccg atgatcgcca tgaacgtgaa tgtcgtgccg
    20701 gtgaggaaca gtgtccagat cacgccgatc acggccatca ggatcgaggt gacgatcgag
    20761 atcggcagga tcgtcgattc gaacagcgcc gccatcacga ggtagatcat cgcgaccgcg
    20821 aacagcaggt tgaagatcat cgcctgcgtg ccgtcgtcgc tctgctgcac gccgcgcccg
    20881 aactgccagg agtagcctgg cggcaactgg taggccgcca tcatcttcgc tacccgttcc
    20941 ctgacgaccg gcagcgtcgc gtcgtcggcg atcacgccgc cgagggtcac ggaggtgagg
    21001 cggttgacgc gctggatcgc gcgcggaccg cgttcgatgg cgaagtccgc gatggcgccg
    21061 agctcgatgc gctggcccga aggcaggtag agcggcaggc gcgcgaggtc ggcgatgttc
    21121 tggcgatcgg actcgcgaaa ggcgaggcgc aaggtcagct cgcggtcggc ggagcggaac
    21181 tcgcgcagcc ggtcgccgcg cagcgccgcc gcgatcgaca cggccgcatc ctgcggcgtc
    21241 acgccgaggg cggcggcccg attgcgatcc acactgatgc gcacctcgtc gtcgccgtcg
    21301 cgggcctcgg agcggatcga ggtgagtccc tcgatgccgc gcagcgcccg ggcgacgtcg
    21361 gcggcgagta cggcgaggcg ctcggtcgac tcgccggtta tgcgcatcga aaagccgccg
    21421 gtggctgccg cgccttccag attgaatgtg ggcttgccga tgatgatctc tggcatgtcg
    21481 gcgcttgccc ggcgcatcac ctcggacgcg ggaatgcgca tatcctcctt agaggtcagc
    21541 ttcaggatgc tgttggccga actcgcgtcg tagcgcgagt agacgctctc gatgtcgaac
    21601 tcctcgcggt gctcggcgag gtagctctcg accgtcttga ccgccgcctc gacccggtcg
    21661 atcggatgcg tgccgccgat gcggtagtcg atgtacagct cgcggcccgc gtcctgcggg
    21721 aacatgtcga aacgcagcag cttgtcgggg aacatgcccg aggcgaccag caggccgatc
    21781 gtcagcgcga gcaggacgcc gagtgccgcg gccgggcgac gcgggtgggt cagcgatgcg
    21841 cgcaggatcg cactgtagcg gtcctgcacg cgcgcgaaga cgctgctgtg cgcgatcggg
    21901 ggcgggggcg ggaagcgcga tgtcagcatc ggcacgatcg tctgggcgat caccagcgag
    21961 gcgctcatcg ctaccacgat cggcaccgcg acgtgcgaca ggaagatgct cagctggttg
    22021 cgttcgccga acaggatcgg caggaagacg acgacgcagg tggcggtgcc cgccagggtc
    22081 gcgatgccga cttcgcgcac gccggcgagc gtggcgccga gcggattggc cgggtcgagc
    22141 tggcggtggc ggaatacgct ctcggtcacg acgacggcat tgtccaccag catgccgatg
    22201 gcgagcatca tgcccatcat cgtcatgacg ttgatcgaca ggtccatgaa gtacatcgcg
    22261 gcgagcgtca ccagcagcga cagcggaacg gcgaggctca cgatcagggt cgtcggccag
    22321 tgccgcagga agagatagag cacgatcagc gcgagcacgg cgccgatgag gccggcttcg
    22381 ccgacatcgg cgagcgaact gcggatcgac gtcgcctggt tttctatcac gtagatcgtg
    22441 acgccctgca gctgcggcag gtcgtgcgcg agctcgagga cctcgagcac gctgtcggcg
    22501 acgtcgatga cgttggcctg ggtggtcttg aacacgtcga ggccgaccgc cgggctgcca
    22561 ttcagccgcc ggcgcgtggt caactccggc gtgaccagtt ccacctcggc gacatcctgc
    22621 agccgcaggt cgggaccaat caccagcttg cgcagctcct cgagcgagcg cagctcgccg
    22681 atggggcgca ccgtgaagcg ctcggtgcca tcggtcatca gaccggcgct caccgagaag
    22741 ttggcgcgct gcagtttctg caccagctcg cgcacgtcga tgcgatgcgc cgcgatgcgg
    22801 cctgcatcga ccagcacacg cacctcgcgc ggttcgacgc cggccagctc gacgcgcgcg
    22861 acaccgtcga cgcgctcgat cggtttcttg agcgtgcgct cgagcacttc gtactgcgag
    22921 gacaggtcgg agtctgccga caggcgtatc gtcagcaccg actggtcggc gctcgaggca
    22981 cggaacatca ggatgcggtt ggcggccgcg ggcaggtcgt gccggatcgc atcgagcttg
    23041 gtgcgcacct cgaagccggc cgcctcgaca tcggtgcccc agtcgaactc gatctcgaac
    23101 gtggcctggt cgctctcgga cttcgagcgg atctcgcgga tgccgggcag cgtggcgagc
    23161 gcatcctcga ccgggcgcgt gatctcgtcc tcgatctcct ccggcgtgga gccggggtag
    23221 ggaatcgtaa cccccatgaa cgggaacgtg atgtccggga actgttcgag cggcagcagg
    23281 cgcgcggcca tcgcgccgat ggtcgccacg gccgcgaaca ccatgatggt cgtgaccggg
    23341 cgacgcagcg cgaagttggt gtgaatcatg ggcgtgcacc cgccgcgacc ggcgcgggta
    23401 cgggcgctgc agcgccggca tcctgcggca ggcgcctgcg atcgagcaac gtgtacagca
    23461 ccgggatgat gatcagcgtc agcacggtcg cgatcagcat gccgccgatc accgtgaccg
    23521 ccatcggccg gcgtacttca gcgccctcac cgaggccgat ggccatcggc agcaggccga
    23581 ggatcgtgct gacgctcgtg atcagaatcg ggcgcaggcg cgtgcgcccg gccagcacca
    23641 gcgcatcgtg gcgggtcagg ccgcgcgcgc gcgcctggtt gacggcatcg agcagtacga
    23701 ttgcgttgtt gacgacgatg cccgcgagca ggatcaggcc gatgaaggcc accgcattga
    23761 tcgtcgtgcc cgtcaccgcg agaccccaca ccgcgccgat gaggcccatc gggatcgtga
    23821 agaggatcac gaacgggtgc agcagcgact cgaactgcga ggccatcacg aggtagacga
    23881 ggaacacggc gagcgcgaac gccatcaaca gcgaacgaaa gctctgctgc atctcctcgc
    23941 tctggccggt gacgccgtag gtgatgccgg cagggagcgg tgtcgttgcg agaatctcgt
    24001 tggcgatggc cgtagcggcg ccgaggttgc cctgggcggg cgcggcggac agcagggcga
    24061 cgcgctcctg tcccgcgcgg cggatctccg ccggcccgat ggcgaggcgc acatcggcca
    24121 ccgccgcgag cgtcacgggc ttctcggcgc ccgggttgac gatcaggttg cgcacctcct
    24181 cgatcgaggc gctgcgggtg tcgacgctgc gcaccagcac gtcgatcttc cggtcgcgaa
    24241 gctggtagcg cgtggccacg tcgccgcgca ggctcgagac cacgctgtcc gcgatggtgc
    24301 gcacctcgag gcccagccgc gaggcgcgat cctggtcgaa caggatctgg atctccggat
    24361 gcccaccctc gatcgaggag cgcaggtcgg tgaacgcgcc gcttgccgcc atgcgcgcgt
    24421 gcacggctgc cgcggcgctc tgcagccgct cgagctcgta gcctgacagc acgacctcga
    24481 gcggcgtcga cagcgtgaac agggcggggc gcgagaactc gtactgcacg ccgggcaggg
    24541 cgtcgagcgc cgagcgcagc tgggccatgg cggcggcctc ctcgcgcggg ccagccggtt
    24601 ggcgcaggcg tatcgacatc tcgccgatgt tctcgccgga atcgaccgga ttggcatcga
    24661 gacggttgcc ggtgccggcc accgcgtagg cgtgatcgag tcccggcaga tcgaccgcgg
    24721 ccgagagtgc ggcgcgcagc acgcggtcgg tgctgtcgag cggcgagccg gcgggcaggc
    24781 gcagcttgac gttgaactcg ccctgcgcaa gctgcgggat cagttcggtg ggcaggcgca
    24841 ccagtatcag cccggtgatc gcgagcagcg agattgcgac gccgagcacc gccgagcggt
    24901 ggcgcagcga ccactcgagc gaggcggggt agagccgctc gagcgccgcg aatccgcgct
    24961 gggtgacatg catggccggg gcgagcacca atccgcacag cgcgccgacc gcgccggcaa
    25021 gcgcgcgggc gagcctgatc aaggtgacga gcagcagtcg cagcggccag gcgagccagg
    25081 cgaagatgcg ccgcaggccg cgcgcggcgg gcgcccgccc gaactcgccc gggcgcagtg
    25141 cgcgcacgat ctcgcgcagc cgcccggcga agatcgcgcc gcgctcggcg agctccgctc
    25201 gcagttcgtc gcgcggcacg cgccgctcgc catcgttcgc acgccacgcc gccatcatcg
    25261 gtacgagcgt caggctcgcg agcagcgaga ggagctgcgc gaacgtcacc gtcagcgcct
    25321 ggtcgcggaa cagctgcccg gcgatgccgc tgacgaacac catcgggaag aacaccgcca
    25381 ccgtggtcag cgtcgaggcg gtgaccgcca tcgccacctc ggcggtgccc tcgcgcgccg
    25441 cctggaccgc gggcatgcct gcctcgcgct tgcgcgagat cgcctcgagg atcaccaccg
    25501 agttgtcgat cagcatgccg accgacagcg cgatgccgcc gagtgacatg atgttgagtg
    25561 tcaggtcgaa gccgtacatc agcacgaaga tgccgatgac ggtcacggga atgacgatgc
    25621 cggcgaccag cgtcgcgcgc gcatcgcgca ggaacaggta cagcacgatc accgacagca
    25681 gcccgcccca gatggccgca tcgctgacct cgctcaccga gttggcgatg aactgcgact
    25741 ggtcgtagac ggtctgcagc ttcgtctccg gcggcagcga cttctgcagc gtcgcaaggc
    25801 gcgcccgcac ggtctcggcc agcagcacgg tgttggcatc gccttccttg tagacggcga
    25861 gttcgacgca ttcgcgcccg tcgagccgcg tgatggccgt ccggtccttg tagccgtgct
    25921 cgacctgcgc gatgtcgcgc agatagaccg ggcggccttc gaccgtcgcg atcaccgcgc
    25981 tcgacatttc ctcgagcgac tggaactcgt tcagcgtgcg caccaggaag cgctgcgtgc
    26041 cctgttcgag gcggccgccc gagagattga cgttctcggc gccgatgcgc gcggcgacgc
    26101 tcgccacgtc gagtttcagc tgcgcgagcc gttcctggtc gacgagaatc cggatttcgt
    26161 cctcgaagcc gccgctcacc ttgaccgccg cggagccttc ggtcgcctcg agttcgggct
    26221 tcaggcgatc gtcggcgtat cggcgcagcg tcttgagccg ctcggaggat gcagcgctgc
    26281 cttcggccag gtcgacgaac gcgaggcgca gcaccggctc ggtcgaggga tcgaagcgca
    26341 gcagcagcgg tcgctccgcg tcggtgggca gctgcagcag gtcgagtcgc tcgcgcacct
    26401 cgatgccggc gaaatccatg tccgtgcccc aggcgaactc gaggaccacg tcggcctggc
    26461 ccgagcgcga caccgagcgc acgctgcgca cgttcctgat cgtgccggcg gcttcctcga
    26521 tcggcctcgt gatcaggttc tcgacctcga gcggcgcggc gcccggcagt tcggtgcgta
    26581 tcgtgagcgt cggatacgag aggtccggca gcaggttgac cttcaaccgt gacagcgaga
    26641 cggcgccgaa cagtgcgacg gcgacggtgg ccatcgcgat cgtgacgcgg cgacggaggg
    26701 cgagatctac gatgcgcata cggacccccg gggagaacta gcggttggcc gtctgtgcgc
    26761 cggcggctac cttgccggcg gcggccgcgc gcagcgccga agtgggcctg gtctcgagcg
    26821 acaccacgcg caccgcgttg ccgtccttga ggcccgtgtg gccgacgacc acgaccgaat
    26881 cgccgtcggc gacacccgtg gttacttcga tgttgccccc gtcgacgaga cccgtggtga
    26941 ccgggcgctg cacggccttg ccatcttcga ccacgaagac gctgctcgtg ccgtcggcct
    27001 cgatcagcgc gacgcgcggc acctgcagcg ccgcgtcgcg ccgctcgaac acgatgccca
    27061 cgcgggcaaa catgccgggc ttcagcacgc catcctggtc atcgagttcg agggtgacct
    27121 tgaaggtggc cgtggccgga tcgatcgacg gcgacacgag ggtcacgcgg gcggggaagc
    27181 tgcggctgcc cagggcgtcg accagcgcga cggcgctctg gcccggtgcg aggtgtgcaa
    27241 gctcgcgctc cggtacgtac acgctcgcct tgagcggcgt cggatcggtg acgcggaagg
    27301 tcttggtgcc ctggggcacg ttctggccga ccttcacgaa ccgcgaggcg acgagcccgg
    27361 cgaacggtgc gcggatctcg gtgtacgaga gcatgagttc cgcgagttcg taggtcgcgc
    27421 gcaggttctc gagatcgaac ttgaggcctt cgaaggcgcc ggccgagacc agtcccttgt
    27481 catgcagctc gacctggcgg cggtagtcgc gctcggcctt ggcgaactgc gcgcgggcct
    27541 gcgcggcctc gagccgcagc tggcggccat cgagcacggc gagcagctgg ccctccttga
    27601 cgc
//
LOCUS       DAOQVY010000022        27421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_2053_length_27421_cov_6.299240, whole genome shotgun sequence.
ACCESSION   DAOQVY010000022 DAOQVY010000000
VERSION     DAOQVY010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 27421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27421
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2053_length_27421_cov_6.299240"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PKN91_03650"
     CDS             <1..294
                     /locus_tag="PKN91_03650"
                     /inference="COORDINATES: protein motif:HMM:NF014396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HNS27198.1"
                     /translation="TGYIVLSNQGADNYAVFRREGDNPLVGYFSIVANEALGIDGVSE
                     TDGLDVTSAPLGPQFPQGLLVVQDGRNIGPTEPQNFKYVSWADVLAALGEEAR"
     gene            complement(340..1695)
                     /locus_tag="PKN91_03655"
     CDS             complement(340..1695)
                     /locus_tag="PKN91_03655"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNS27199.1"
                     /translation="MIDALRRRLLGAAAGAAGVAMLPKRAYAAPLGYPRLLQGPMIGD
                     TGPTHLTIWGRATGPLPIAFQYSKDRAFTAPLTSATVATSAASDFTAAVRIDGLEPDT
                     RYWYRPVVDGVVDRYQSVPVATRTAPAGAADFRVAFGSCARIARDPVQRIFDIVVRES
                     PDLFLWLGDNIYADSEAPEAIADEYRRQRKVQSLAPLIRSVPQLAIWDDHDYGYNNGD
                     GSSPFKAASLATFRNYWANPAYGLPGTPGVFFEKHFGGIDFFMLDGRYHRSPNGDPDG
                     PAKTFLGAAQNAWLRERLAASRAPFKILACGSGWSMADGEKGDTWAAFRTERDALFDF
                     IRDRGIEGVVLLSGDSHVGELNCIPWSERGGYDLYDLVSSPLAQSPSSSWIDQQPEVR
                     MRKVYAGGANFGMLHFTPGPQPTLTFSLFDEWGLMPWKPLVLVPADLRNGVRSFERLR
                     A"
     gene            complement(1692..2594)
                     /gene="iolE"
                     /locus_tag="PKN91_03660"
     CDS             complement(1692..2594)
                     /gene="iolE"
                     /locus_tag="PKN91_03660"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR04379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HNS27200.1"
                     /translation="MTIELGINPITWTNDDLPGLGADIPLETCLREARAAGYTGVELG
                     RKFPRDARSLGPILAAHDLRLVSGWYSLRLLERDIDAEFAAMAAHLALLAALGSTVMV
                     VCEVTGCIHGELGARLSRRPHMPPQRWAEFGDRLTRLARRMQDAGMRMAYHHHMGTVV
                     QSGADVDALMKHTGEEVGLLLDTGHLTYAGENPARFATSYAARIVHVHCKDVRAEVLA
                     RARNRDASFLDAVLQGVFTVPGDGSVDFPAALGPLARAGYDGWLVVEAEQDPAVATPS
                     VYAAKGHSYLARITSEIFDQGAAR"
     gene            complement(2591..4414)
                     /gene="iolD"
                     /locus_tag="PKN91_03665"
     CDS             complement(2591..4414)
                     /gene="iolD"
                     /locus_tag="PKN91_03665"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR04377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HNS27201.1"
                     /translation="MKYERLTTAQALVRYLAAQRIREPGGEVPLFAGMFAIFGHGNVA
                     GIGEALQHVRGTFPTWRAHNEQAMAHAAIAFAKATRRRRMLACTTSIGPGATNMVTAA
                     ALAHVNRLPVLLLPGETFANREPDPVLQQVEHFGDPTVTANDCFRPVSRFWDRISRPG
                     QLLASLPQALSVLTDPADCGPVTLALPQDVQAEAHDWPVAFFEPRIHERVRHGPDAQQ
                     LRRAVELLRAARKPLIIAGGGVLYADACEALAAFALARGIPVSETQAGKGALPWDHPC
                     NVGAIGVTGTGVANALAQEADLIIAIGTRLADFTTGSGQLLPREGCRLLSLNVCRHDL
                     AKRGAAPLQADALRGIETLDAALGAPQARGRWLADAQARIQEWNAARDAAVAARGSGL
                     PSDAEVLEIANRAAGARGTVVCAAGGLPGELHKLWRASGPGSYHVEYGYSCMGYEIAG
                     GLGVKMAQPDRDVIVLVGDGSYLMMNSEIATSIALGQKLTIVVLDNRGYGCINRLQGD
                     CGGDSFNNLLDDAAPRVDFVAHARALGAHAEKATDLTALATQLARAIRADRTAVVVID
                     TDPAHSTAVGGAWWNVPVAEVSASTRVRDAHADYRRHLDES"
     gene            complement(4411..6324)
                     /gene="iolC"
                     /locus_tag="PKN91_03670"
     CDS             complement(4411..6324)
                     /gene="iolC"
                     /locus_tag="PKN91_03670"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: protein motif:HMM:TIGR04382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HNS27202.1"
                     /translation="MTEAVAAGTRALDVICLGRAAVDLYGQQLGGRFEDVQTFAKYLG
                     GSSANLAFGLARLGLKSSMLSRVGDEHMGRFVREALARAGVDVSHLATDPQRLTALVL
                     LGIAGRDDFPHIFYREHCADMGLTGDDFDAAYIASSRMLAITGTHLSSPSTAAAVMKA
                     AELARAHGTRVVLDIDYRPVLWGLVSAGEGASRFVASGSVTARMQPLLPCCDLIVGTE
                     EEIRVAGGDQDLDTAVRAIRATSAATLVVKRGAAGCTVLPAAGECLDVPGFPVEVLNT
                     LGAGDAFLAGLLSGYLAGADWQRAATIGNACGALVVSRHGCAPALPSQVEIEDFLARA
                     PAITRPDLDLRLQQLHDATTARRPRKDLYVLAFDHRRQLEDLAGSWGLPVTHINRLKE
                     LVADAVERVAARVTYSDRLGVIVDERYGETVLSRMTHAGYWVGRAIEIPGSRPLELEP
                     RNSAGLPLLGWPANQVVKCLVFYHPDDPLELRLAQEERVAGLWHDTQALGRELLLEVV
                     ASGHGRQTDDASLARTLRRFYNLGIRPAWWKLEPPSPAAWPRIQDVIMEFDPYCNGVL
                     LLGLDAPESQLRAGFEQAAPFPICRGFAVGRSIFGHSAREWMRGQVGDAQLVEDIATR
                     YERLIDIWRRLRP"
     gene            complement(6317..7129)
                     /locus_tag="PKN91_03675"
     CDS             complement(6317..7129)
                     /locus_tag="PKN91_03675"
                     /inference="COORDINATES: protein motif:HMM:NF016826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HNS27203.1"
                     /translation="MNHISTHRQGFPAGLTAITRAADPDNATGLGLAVMILAAGQSWS
                     DTVDGETAWLLMSGSVRVRACEEAAVLTRRSLFDESASCLHVSAGTRVTLQAESDCEL
                     TVYSCANTARFPPRIFRPADVPNEHRGKGQVRDRSLRFVRTIFDGRNSPPAAGLVLGE
                     VVTFPGGWSSYPPHHHPQPEIYHYRFTAPQGYGHAELGDEVVKVRQFDSVCIPAGYDH
                     AQCAAPGYGMYYSWVIRHLPGAPYTVPEFTPEHAWTMAKDASFWWPAEGGDD"
     gene            complement(7126..8250)
                     /locus_tag="PKN91_03680"
     CDS             complement(7126..8250)
                     /locus_tag="PKN91_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNS27204.1"
                     /translation="MTRRRFGLIGTGFIGRAHAIALNAVGAVFVEREAPVCELLADSS
                     PEAAAQAARALGFARSTGDWRDVVADPAVDVVDICTPNFLHREMALAAIAAGKHVYCE
                     KPLALTAREAHEIAAAADAAGVTHMMGFNYVCNPLLQVARDMIAAGELGEVYAFRGSY
                     FEDYMSDPAAPWTWRCKRELAGSGALADLGSHLINLGHLLLGGIARVQGSLQLVHATR
                     RDPASGEWRAVENEDIARALVEFEGGVPGSFEISRVATGYKCGLACTVFGTRGTIEFD
                     QERMNELRWYDAAGPAARSGFRTILAGPEHPDYRPFCPAPGHGLGYNDLKVIEVRNLL
                     RALDGEARAWPDFHEGARVQRVMEAIEQSAATRSWTEIPA"
     gene            complement(8264..9259)
                     /gene="iolG"
                     /locus_tag="PKN91_03685"
     CDS             complement(8264..9259)
                     /gene="iolG"
                     /locus_tag="PKN91_03685"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HNS27205.1"
                     /translation="MKYGFALFGAGRIGQIHARNLAAHADAQLRHVVDVSAEAARRLA
                     GASGAQVSDARAALADPAVHAILIASSTDTHAELIQAGARAGKAVFCEKPLDLDIARA
                     EASLAVVAEAKTLLALGFNRRYDPAFRRLQRELAAGRVGNVEVVSITSRDPSPPPPAY
                     VARSGGLFRDMMIHDLDMARWLLAEEPVSVYARGSALIDPAIGAAGDIDTAVVVLETA
                     SGRLCQITNSRRCAFGYDQRIEVFGSAGLVRADNVPATTVETADASGFTREPALPFFL
                     ERYAAAYRDELDEFLKALRGEPAQLATGSDGRQALRLADAAQRSLELRAPVSTLD"
     gene            complement(9256..10113)
                     /locus_tag="PKN91_03690"
     CDS             complement(9256..10113)
                     /locus_tag="PKN91_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HNS27206.1"
                     /translation="MAAPKDLERLRADIVRRYEELSPRLQQVAQFALDHPNDMGLQTL
                     AVIAERCNVQPSTVVRFAKAMGYEGASDMQKLFRDELLTFAPSPSYADRIRQFDDRSG
                     AVDVLAPQELLQEFADANIIALEHLKGSIRKADLEKAVALIREAAAVYIIGLRRSFPV
                     ASYLAYALRHVDEKRAYLLDGVAGMLAEQSGMARQNDLLIAISFRPYAAETAEIVAQA
                     RARGARIIAISDTRVSPIARDADVVFETKDAEVLQFRSLTASLCLAQALVVSYAFQSE
                     RRASKGKSR"
     gene            10324..13119
                     /locus_tag="PKN91_03695"
     CDS             10324..13119
                     /locus_tag="PKN91_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS27207.1"
                     /translation="MKLRQSSLVAAAIAAALAGHAGNSLAGELAGQVASSAGESLPNA
                     TVRIPELNRSVRADRSGAFRFTDVPAGVYTVVAEYVGFGQASGSVTVAETGVATQNFL
                     LTGSNIEEVTVTGFRLAQATALQDKKSAKYIKESVTADDAGKLPDRNAGDALARVTGV
                     SVTTDQGEGRYVNIRGIDAALSNVTIDGQTIGSPEGDTRRIALDTVPSNILAKLEVIK
                     SVTPDLDGNAIGGTVNLVTPSAFDDPKGRSFAMSLDYGYSDLGGENPWGASAAWSGVF
                     ADDRLGVILSASYSDREFNSQNMQGGDPWQEEGDFLVPDELNLRDYVIRRVRKGLVAN
                     FEFRPNDATKLHWRNIINRYEDTELQPEVTYDFRNGDLEDQTATSGLFTEGEGERANS
                     ERFEVQELLSSTLGGEFELGNWNLNVVLTYGESEQDTPYDNFYAFESADEMPMTYDTS
                     DRFWQVGAPEEFYDAGSFEFSEAARGGQLIKEELNIAQLDLLRNVEWGGGNSGYIKFG
                     GKFASRESTSDQDMIVYDGFDGDLLLTQVAAAGDPGFYRDVRPYYRFGPYPDYGASED
                     FFRANEGLFEVSDADTSIESYGVDYTVKEDVTAGYIMGSADIGNVTLVGGVRVERTDT
                     DFTAFDVQIVDGDALDPPPQVIGSKDYTNWMPGLQATWAIRPDLIARAAWTNTIGRPS
                     YEQNVPFRIFETEEDDPGIFEGAIEMGNADLDPLESANYDLAIEWYLRPSGLLSAGVF
                     FKDIDNPIFTRVLTLEDQDFEGRFYSELEVVQPQNARSGDIFGIELAYQQQFSMLPGF
                     LRGFGISIGYTYTDSEAETADRTEKVPFFLQSDHVGNVALFYELSGLELRLGYAYRSE
                     YLVELGDSAAEDLYIDTHGQLDFKASYQVTSKLGAFVEMKNITDEPLRYFSGDRSRMA
                     ENEFYSWTILAGVNLKF"
     gene            13168..14037
                     /locus_tag="PKN91_03700"
     CDS             13168..14037
                     /locus_tag="PKN91_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNS27208.1"
                     /translation="MPNRPLRGIAFMTAAVAVFAVMDALLKALTARYPPLQVAALRGA
                     ASLPFIALPLLLSGRAATLMPRRPGMHLLRGLLMVVVMGGFLYAVRSLSLADAYSIFL
                     TAPLMVTALAALVLHEHVGWRRWLAILIGLGGALVMLRPSASGFATVAAIGALVAALG
                     YACNAILLRVLTRTDTTASVALWSIAVMTVVAAAIALPAWVTVGRTDIWLIIALGVVG
                     AVAQMCMIEAFRAAPASVIAPFEYSALFWGICIDWVVWQALPTLRVLAGGGIVIGCGL
                     YIIWRERELTMRR"
     gene            complement(14021..15526)
                     /locus_tag="PKN91_03705"
     CDS             complement(14021..15526)
                     /locus_tag="PKN91_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNS27209.1"
                     /translation="MPAIQSPAPAAATQAFLARTHQLFIDGKWVNPLAGGRLDVYDPA
                     TGGKIATAAAGEAPDIDAAVAAARAAFTGPWARFTAAERGNVLWKLADLIERDAPLLT
                     ELEVLDNGQPLAIAQLVVNRLAPEFFRYYAGWPTKIEGATIPAAPQGRRPGEVLSFTL
                     REPIGVVGAITPWNAPLPMLMVKLAPVLATGCTIVLKPAEHTPLTALRIAELCAEAGV
                     PDGVVNVVTGLGETAGAALAAHPGVDKIAFTGSTEVGRMIVRAAAGNLKKVSLELGGK
                     SPVIVFPDADLEQVIPGAFRACFFLQGQNCMAGTRLFVHEKVHDEVVGGVAAIARGIR
                     LGHGLDPASQFGPLISAEQRDRVRGFVEAGTREGGELVCGGSAPARSGWFFEPTIFTR
                     MHAGMTPVMNEIFGPVLCVQSFGDDEIERIANEANDTIYGLSGSVWTRDIGRALQMVR
                     RIDSGQVSVNMHAALDPAMPFGGNKQSGWGREFGKEGLEAYLKTKGVSVAW"
     gene            complement(15632..17620)
                     /locus_tag="PKN91_03710"
     CDS             complement(15632..17620)
                     /locus_tag="PKN91_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNS27210.1"
                     /translation="MTQFLNMLRSAWPRFRLLTTLLAWYLVVGLLLRAALWWSFGRPA
                     EVSDVAFVGTLAAGGAADIVQALYLLAPFALLAWLAPDRWFRTRGMRVTILALAALFV
                     FQMGFVAVAEYYFFEEFDSRLNLVSVDYLMYPTEVVGDIRSEYPVIPIVLVVALLAAA
                     TAYVLRRPLLAGTRAASGFGARSATALVYALLVATAVVAIDRDSFSFSTNRVTNEIAA
                     NGAASFFNALRTSEIDYHAYYATADRAANLDALRTTLARGGGRLVDDRGIDRTFPARP
                     DGLGRLNVVVIVSESFGAEFSRAFGGQHDWTPYFDEYARQGIMFTNMYASGTRTVRGL
                     EAITASIPPIPTTSILHRPGNTGIANWGAVMRELGYSTSFFYGGYGSFDGMNAFFAGN
                     GFEVADRREIDTPVRFENIWGVADEDLYDLAMRRFDALADTGKPFFAIVMNTSNHKPF
                     TFRPGVPGVKAEGGGRESGVRYADYAQAYFLREAAAHRWFDDTVFVIVADHGARVYGR
                     EDIPLRTYRIPMLVYAPKHIAPERVDTLTTQIDVAPTVLGLLGLPYRAPFFGQDVRHA
                     PPTDEPIALFSHNHDVALLREHELAILGLKKAVTNLSYDAANDAYAPAPADRRLDALA
                     IAYYQTAYEMFREHRYVPTPAAPGDTHLATASVAAGAH"
     gene            complement(17623..19245)
                     /locus_tag="PKN91_03715"
     CDS             complement(17623..19245)
                     /locus_tag="PKN91_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNS27211.1"
                     /translation="MRTAAFIIVLLAASRIAAGSPDPVAIPPDEVLERAGARIGAIEI
                     DCESIFDENDPREDSLLYRTANRLHVETREATIRAQLLFASGDPYSRRLLAESERVLR
                     RQRYLHEPAIRPVAWHDGVVDVRVRASDVWTTNPGVSVSRSGGANRLGAEIEELNLFG
                     RGKQLSAGYTSDVDRTSIGVTWHDPAVFGSRWTSDAGYTGSDDGRFWALGVDRPFYAL
                     DARWSAGFSAAAGTQIDPRYSLGERADAYRSHARRFEVHRGWSSGLRDGWVRRWSAGY
                     RYDSVEFESLPDTALLAPLPADRTLAYPFARFELLQDDFVTARNLEQIGRTEDLRFGT
                     RLTAELGWSQSAFGADRNAFIASLGASRGYRLGPGQNLFLSAGWNGRLEAGQLRDSLL
                     SAEARYFWRTGERTVLFVGLAANHGAALDADHELQLGGDTGVRGYPLRYQTGTGRALA
                     TIEARYFTDWYPFRLARVGAAVFADVGRTWGPSALDVPALGTLRDVGIGLRFGNTRSA
                     LGSVLHVDLAFPLDGDPSLHSMQFLLKTHKSF"
     gene            19324..19920
                     /locus_tag="PKN91_03720"
     CDS             19324..19920
                     /locus_tag="PKN91_03720"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNS27212.1"
                     /translation="MQQDDRALVARLLAGDDDAFRSFFDEYFDRLYRFALSRTSGRTD
                     LAEEAAQRTLCRALRALQQYRGEASLFTWLAQICRNELADLGESERRDAERQQSYDAS
                     ERARQLAEGVASADAAPAERAQAAVASDILRRALDALPGRYGDILEWKYLEDWSVQQI
                     AAELGASFESAQSSLARARLALRSALVASGIDWHELLP"
     gene            19917..20888
                     /locus_tag="PKN91_03725"
     CDS             19917..20888
                     /locus_tag="PKN91_03725"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNS27213.1"
                     /translation="MTERTGDWTTADEARLARLMRSLPRGTPDPAARARAFEAAQAEW
                     RRSRARPLRGAPVRLAGWAAAVAVLAIGVALWVQPYAAPVAHLDRVYGSVSADADPLE
                     VGARLRRGAAVETAQESGALLRYSPDLTVRLDAGTRVAMVDRATLRLDAGRVYIAVAP
                     GTAGAFVVRTALGDVRHVGTRYAVSARDGELEVAVREGEVRIDLGARAERATAGEALR
                     IDPEGAILRAVLSADDARWAWIDSLPSPVAIEGRTLVEFLQWYAVETGRRVDYADDAT
                     RARAQATILHGSVDGLAPAQALAIVASSADVEAILRDGQLVIGPARR"
     gene            20912..23242
                     /locus_tag="PKN91_03730"
     CDS             20912..23242
                     /locus_tag="PKN91_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS27214.1"
                     /translation="MPGTLRTRAPAWSTLLLLFASALAAGVEVGEPLGAALDRLAGSG
                     LRLVYSTALVTPDLRVRAVPRPGPSVEMARELLAQHGLALEPVQPGVYVVVRAAAGDA
                     TAGGSVAGRGGAPVGDAAAPLIEVSILASRYRIDQQSEFAPVEITGEDLAARPGVDAD
                     ALRVTRLLPGTATNGLSARAYVRGGRADELGVWFDGVPLFEPFHFKDFQGLLGILDPA
                     TVGRLDFWSGVHPARFGGRLSGVLDIAPRAWNDENHHEIGASLLYAHALSQGRLEAAP
                     LEWLVAVRKSTIDQIMKLAEERTGEPEFLDLLVRLAWRFDGGSDVAAGWLVLDDGLTA
                     NASDDTERARARYRDGTGWLRGTWRIDDDLEVRGLVSRTERHTRRVGTVDRAGSATGA
                     LEDRRFMDANTARLELGGRHGRVRYTLGGELMDHDTSYEYAANAVFDPLLAAALGRPD
                     ALSHDLTLVAGGQSYAAYASALIGLSPRLDVDLGVRWDRQDYRPGFDDSQWSPRVGIE
                     YRPREGSALRVSFGHLSQPERPDELQVTDGDPQFHAVQTATQAVVALEHRLAKDQRIR
                     AEVFDKRMSHVRPVHENILDPVTLLPEIEVDRVPVAPRSSRAYGAELSWYWEPRGPWS
                     ASLGYSWSEVTDHFDDSRALRSWDQRHAAVAGLTWTRDPWQLSGSLFWHSGWRRNDLR
                     VEVSPEGEAQLVLSPRNAGTWPDYLSLDLRASWQRPLPVGTLQVFAEISNLANNDNYC
                     CTEYHLAVPGDAASLEGERSTWLPRYGLIGVKWILP"
     gene            complement(23247..24935)
                     /locus_tag="PKN91_03735"
     CDS             complement(23247..24935)
                     /locus_tag="PKN91_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNS27215.1"
                     /translation="MRRAEDLQHYAWLALALLALLATGLGIRDPWPADEPRFALIARD
                     MVASGDWLFPRVGGELYPDKPPLYLWMIALGLSLTGSLRIAFLLPSLLAACATVALVY
                     DLARRLAGREAALAAAFTLACTLQFVLQGRAAQIDATVAFLITLGCYGLTRHMLLGPA
                     WGWYFAGGFAAGLGVITKGVGFLPLLLLIPYAILRRRRWQGLAPSAAGGWRWALAPLG
                     LLAAIALWLVPMVWAVARHGTPEFAAYRDEILFHQTLTRYGASWHHVKAWYYYLVEVI
                     PALWLPWSILAFWLVPGWRAAWRARDARIWLPLSWVALVLLFFTLSPGKRGVYLLPAL
                     PGLALAAAPLLPAVLARRGVAVASLALSGVLLVAVAGIAIAVALDLPQAHTLLSAENL
                     PPDLPMYVPAFACAAAWLLAWRRPLLGWPLVLAALAASFSWAIAPAIDPIRSGRAFMQ
                     HVLAQVPPDRELGLVAYKEQFLLHLDRPIVNFGHRRWREDGAEEADAARWLAAAPGRV
                     LLVPDALLAPCFTTATAHGARTSAGERWSLVTGSPAPDCVARGDASRAIRYAPP"
     gene            complement(24922..25182)
                     /locus_tag="PKN91_03740"
     CDS             complement(24922..25182)
                     /locus_tag="PKN91_03740"
                     /inference="COORDINATES: protein motif:HMM:NF019214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HNS27216.1"
                     /translation="MTHETVWIVIGFVAQALFSARFFVQWIASERRGRSIVPHAFWYF
                     SVAGGALLLAYAIHRRDPVFIVGQGAGLIVYVRNLQLLRAAR"
     gene            complement(25179..25925)
                     /locus_tag="PKN91_03745"
     CDS             complement(25179..25925)
                     /locus_tag="PKN91_03745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015066033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS27217.1"
                     /translation="MSNQVEVSVVVPVCDEVENVEPLAGEIATALAGRDYEIIFVDDG
                     SSDGTGERVLRLAAAGVAPLRLLRHSRRAGQSAALCTGVRRARGRRIVTLDGDGQNDP
                     VDIPRLLATHERAGNDRPLLVIGHRQSRRDTAWRRLQSRIANVVRSRLLGDGTPDTGC
                     GLKALDRALFLDLPRFDHMHRFLPALVRHAGGEVVSVPVGHRERRHGRSKYGMLNRLW
                     VGIVDLYGVMWLAHRTWARLEVRELTGEPR"
     gene            26124..26717
                     /locus_tag="PKN91_03750"
                     /pseudo
     CDS             26124..26717
                     /locus_tag="PKN91_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
     gene            complement(26738..>27421)
                     /locus_tag="PKN91_03755"
     CDS             complement(26738..>27421)
                     /locus_tag="PKN91_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNS27218.1"
                     /translation="ADVGKMIRVREATPEILALMASGVSAMVALERIADMKSGEIVAV
                     SAAAGGLGHFVVQLAKRAGNHVIALTGSDHKAALLRELGADRVVNYRTESLREVLARE
                     YPRGLDIAYDSVGGEIFDAFLDHLAMYGRLIVSGHTSDFDKPIEDVPHPRAYHKLYWK
                     SASIRAFQNPAFPQYFDEAARRILDLYYRGELRALVDPTPFTGLESAADAVEYLLAGR
                     NAGKVVLRL"
BASE COUNT         4229 a         9237 c         9766 g         4189 t
ORIGIN      
        1 acgggctaca tcgtgctgtc gaaccagggc gccgacaact acgcggtgtt ccgccgcgag
       61 ggcgacaacc cgctggtggg ctatttctcg atcgtcgcca acgaagcgct cggcatcgac
      121 ggcgtgtcgg aaaccgatgg tctcgacgtc acgagcgcgc cgctgggacc ccagtttccg
      181 caaggtctgc tcgtggtgca ggacggccgc aacatcggcc cgaccgaacc gcagaacttc
      241 aagtacgtct cgtgggcaga cgtccttgcg gcactgggcg aggaggcgcg ctgacgccgg
      301 gcggaggccc cgctggcatg cagtgcgcgg cacccggcct caggccctca gccgctcgaa
      361 cgagcgcacg ccgttgcgca ggtcggcagg cacgagcacg agcggcttcc agggcatgag
      421 tccccactcg tcgaacagcg agaaggtgag cgtgggctgc gggccgggcg tgaaatgcag
      481 catgccgaag ttggcgccgc cggcgtagac cttgcgcatg cgcacctccg gctgctggtc
      541 gatccacgag gacgacggcg actgggcgag cggtgagctc accaggtcgt agaggtcgta
      601 gccaccgcgc tccgaccacg gaatgcagtt cagttcgccg acatggctgt cgccggagag
      661 cagcacgacg ccttcgatgc cgcggtcgcg gatgaagtcg aacagcgcat cgcgctcggt
      721 gcggaaagcc gcccaggtgt cgcccttctc gccgtcggcc atcgaccagc cgctgccgca
      781 ggccaggatc ttgaatggtg cacggcttgc ggcgaggcgc tccctgagcc atgcgttctg
      841 ggcggctccg aggaaagtct tcgcgggccc atccggatcg ccattcggcg agcggtggta
      901 gcggccatcg agcatgaaga aatcgatgcc gccgaagtgc ttctcgaaga acacgccggg
      961 cgtgccgggc aggccgtagg cgggattcgc ccagtagttt ctgaaggtcg cgagcgaggc
     1021 ggccttgaac ggcgagctgc cgtcgccgtt gttgtagccg tagtcgtggt cgtcccagat
     1081 cgcgagctgc ggcacgctgc ggatcagcgg cgcgagcgat tgcaccttgc gttggcggcg
     1141 atactcgtcg gcgatcgcct cgggcgcttc ggagtcggcg tagatattgt cgccgagcca
     1201 gaggaagagg tcgggcgatt cgcgcacgac gatatcgaag atgcgctgca ccggatcgcg
     1261 cgcgatccgc gcgcaggatc cgaaggcgac gcggaagtcg gcggcgccgg ccggcgcggt
     1321 ccgcgtcgcg accggcacgc tctggtagcg gtcgaccacg ccgtcgacca ccggccggta
     1381 ccagtagcgg gtatccggct cgagaccgtc gatgcgcacg gcggcggtga aatcgctggc
     1441 ggcgctcgtg gccacggtcg cgctggtcag aggcgcggtg aaggcgcggt ccttcgagta
     1501 ctggaaggcg atcggcaggg gacccgtggc tcggccccag atcgtgaggt gcgtgggccc
     1561 ggtgtcgccg atcatggggc cttgcagcag gcgcggataa ccgagcgggg cggcgtacgc
     1621 cctcttcggc agcatcgcga ctcccgccgc gcccgcggcg gcaccaagga gtctgcgtcg
     1681 cagggcgtcg atcatcgtgc agccccctgg tcgaagattt cgctcgtgat gcgcgccaga
     1741 tagctgtgac ccttggccgc atacaccgag ggcgttgcga cggccgggtc ctgctcggct
     1801 tcgaccacca gccagccgtc gtagccggcg cgtgcgagcg ggccgagcgc ggccgggaag
     1861 tcgacgctgc cgtcgccggg caccgtgaac acgccctgca gcacggcgtc gaggaagctc
     1921 gcgtcgcgat tgcgcgcgcg cgcgaggact tccgcgcgca catccttgca gtgcacgtgg
     1981 acgatgcgcg cggcgtagct ggtcgcaaag cgcgcggggt tctcgcccgc gtaggtcagg
     2041 tgccccgtat cgagcagcag cccgacctct tcgccggtgt gcttcatcag cgcatcgaca
     2101 tcggcgccgg actgcacgac cgtgcccatg tgatggtgat aggccatgcg catgccggcg
     2161 tcctgcatgc gccgcgcgag ccgtgtcagc cggtcaccga attccgccca gcgctgcggc
     2221 ggcatgtgcg ggcgtcgcga aaggcgcgcg ccgagctcgc cgtggatgca gccggtgact
     2281 tcgcatacca ccatgacggt ggagccgagt gccgcgagca gtgcgagatg cgcagccatc
     2341 gcggcgaact cggcgtcgat gtcgcgctcg agcagccgca ggctgtacca gcccgagacg
     2401 agccgcaggt cgtgcgcggc cagtatcggc ccgaggctgc gcgcgtcgcg cgggaacttg
     2461 cgtccgagtt cgactccggt gtagcccgcc gcgcgcgctt cgcgcaggca ggtctcgagc
     2521 gggatgtcgg cgccgagacc gggcaggtcg tcgttcgtcc aggtgatggg attgatgccg
     2581 agctcgatgg tcatgattcg tccaggtgcc ggcgatagtc cgcatgcgcg tcgcgcaccc
     2641 gcgtgctggc cgagacttcg gcgaccggga cgttccacca cgcgccgccg accgcggtcg
     2701 agtgcgccgg atcggtgtcg atcacgacga cggcggtgcg atcggcgcgg atcgcgcgcg
     2761 cgagctgcgt cgcgagtgcg gtgaggtccg tggccttttc ggcgtgggcg ccgagcgcac
     2821 gggcgtgcgc gacgaagtcg acgcgcggcg cagcatcgtc cagcaggttg ttgaaggagt
     2881 cgccgccaca gtcgccctgc aggcggttga tgcaaccgta gccgcgattg tcgagcacca
     2941 cgatcgtgag cttctggccg agcgcgatcg aggtcgcgat ctccgagttc atcatcaggt
     3001 agctgccgtc gccgacgagc acgatgacgt cgcgatcggg ctgcgccatc ttcacgccca
     3061 ggccgccggc gatctcgtag cccatgcacg agtagccgta ctcgacgtga tagctgcccg
     3121 ggccgctcgc ccgccacagc ttgtgcagct cgcccggcag tccgccggcg gcgcacacga
     3181 cggtaccgcg cgcgccggcg gcgcggttgg cgatctcgag cacttcggca tctgagggca
     3241 gtccggagcc gcgcgcggcg accgcggcat cgcgcgccgc attccactcc tggatgcgcg
     3301 cttgtgcatc ggcgagccag cggccgcgcg cctgcggtgc gccgagcgcc gcgtccagcg
     3361 tctcgatccc gcgcagcgcg tcggcctgca gcggcgcggc gccgcgcttg gcgaggtcgt
     3421 ggcggcacac gttcaacgac agcagcctgc agccctcgcg cggcagcagc tgcccggagc
     3481 cggtggtgaa gtcggcgagc cgcgtgccga tcgcgatgat caggtcggcc tcctgggcga
     3541 gcgcattggc aacgcccgtg ccggtgacgc cgatcgcacc gacgttgcag ggatgatccc
     3601 agggcagcgc acccttgccc gcctgggtct cgctgaccgg gatcccgcgt gcgagcgcga
     3661 atgcggcaag cgcctcgcag gcatccgcgt agagcacgcc gccgccggcg atgatcagcg
     3721 gcttgcgtgc cgcgcggagc agttcgacgg cgcggcgcag ttgctgcgca tcggggccat
     3781 ggcgcacgcg ctcatggatg cgcggctcga agaaggcgac gggccagtcg tgcgcctcgg
     3841 cctgcacatc ctgcggcagc gcgagcgtaa cggggccgca gtcggccgga tcggtcagca
     3901 ccgacagcgc ctgcgggagt gaggcgagca gctggccggg ccgggagatg cggtcccaga
     3961 agcgcgagac cggacggaag cagtcgttcg cggtgaccgt cgggtcgccg aaatgctcga
     4021 cctgctgcag caccggatcg ggttcgcggt tggcgaaggt ttcgccgggc aggagcagca
     4081 cgggcaggcg attgacatgc gccagcgccg ccgcggtcac catgttggtc gcgccgggtc
     4141 ctatcgatgt cgtgcaggcc agcatgcgcc ggcgtcgcgt cgccttcgcg aaggcgatcg
     4201 cggcatgcgc catcgcctgc tcattgtgcg cgcgccaggt cggaaacgtg ccccgcacgt
     4261 gctgcagggc ttcgccgatg ccggcaacgt tgccatgacc gaagatcgcg aacatgccgg
     4321 cgaacagcgg cacttcgccg cccggctcgc ggatgcgctg cgcggcgaga tagcgcacca
     4381 gcgcctgtgc agtggtgagg cgctcgtact tcacggtcgc agtctcctcc agatgtcgat
     4441 caggcgctca tagcgcgtgg cgatgtcctc gaccagctgc gcatcgccca cctgtccgcg
     4501 catccattcg cgcgccgaat ggccgaagat gctgcgcccg accgcgaagc cgcgacagat
     4561 cggaaacggc gcggcctgtt cgaacccggc cctcagctgc gattcgggcg catcgagccc
     4621 gaggagcagc acaccgttgc agtacgggtc gaattccatg atcacgtcct ggatgcgcgg
     4681 ccaggcggcc ggagagggcg gctcgagctt ccaccacgcc ggtcggatgc cgaggttgta
     4741 gaagcgccgc aatgtgcgcg ccaggctcgc gtcgtccgtc tgtcgcccgt gcccgctcgc
     4801 gacgacctcg agcagcagct ccctgccgag cgcctgcgtg tcgtgccaga gtccggcgac
     4861 acgctcttcc tgagcgagcc gcagctcgag cgggtcgtcg ggatgataga acacgaggca
     4921 cttcacgacc tggttggcgg gccagccgag cagtggcagg ccggcgctgt tgcgcggttc
     4981 gagttcgagc ggtcgcgagc ccgggatctc gatggcacgc ccgacccagt agcccgcgtg
     5041 cgtcatgcgc gacagcaccg tctcgccgta gcgttcgtcg acgatcacgc ccagtcgatc
     5101 gctgtacgtg acgcgcgcgg cgacgcgctc gacggcgtcg gccacgagtt ccttgaggcg
     5161 attgatatgt gtgaccggca ggccccagct gccggcgagg tcctcgagct ggcggcggtg
     5221 gtcgaaggcg agcacgtaca ggtccttgcg cgggcggcgc gcggtcgtcg cgtcgtgcag
     5281 ctgctgcagc cgcagatcga ggtcgggccg cgtgatcgcc ggggcgcgtg cgaggaagtc
     5341 ctcgatctcg acctgcgagg gcagcgccgg cgcacagcca tggcgcgaga cgacgagcgc
     5401 gccgcaggca ttgccgatcg tggcggcgcg ctgccagtcg gcgccggcga gatacccgct
     5461 gagcaagccg gcgaggaagg cgtcgcccgc gccgagcgtg ttcagcacct cgaccgggaa
     5521 tccgggcacg tcgaggcact caccggccgc aggaagtacc gtgcagccgg ccgcgccccg
     5581 cttgacgacg agcgtggctg cgctcgtcgc gcgaattgcc cgcacggccg tgtccagatc
     5641 ctggtcgccg ccggcgacgc ggatttcctc ctcggtgccg acgatcaggt cgcagcaggg
     5701 aagtagcggc tgcatgcgcg cggtgacgct acccgaggcc acgaagcgac tggcgccttc
     5761 gccggcggag acgaggcccc acagcaccgg ccggtaatcg atgtcgagca cgacgcgagt
     5821 gccgtgggca cgcgcgagct ccgcggcctt catgaccgca gcggcggtcg acgggctcga
     5881 caggtgcgtg cccgtgatcg cgagcatgcg gctcgaggcg atgtaggcgg cgtcgaagtc
     5941 gtcgcccgtg agccccatgt ccgcgcagtg ctcgcgatag aagatgtgcg ggaagtcatc
     6001 gcggcccgcg atgccgagca ggaccagcgc ggtgagtcgc tgcgggtcgg tcgcgagatg
     6061 gctcacgtcg accccggcgc gcgcgagcgc ctcgcgcacg aagcgaccca tgtgttcgtc
     6121 gccgacccgc gagagcatcg atgatttcag gccgagacgc gcaagtccga aggccaggtt
     6181 ggccgaggag ccgccgaggt acttcgcgaa agtctgcacg tcctcgaaac gcccgccgag
     6241 ctgctggcca tacagatcga cggcggcgcg cccgaggcag atcacgtcga gcgcgcgcgt
     6301 gccggcggca accgcctcag tcatcgccgc cctccgcggg ccaccagaac gaggcatcct
     6361 tcgccatcgt ccacgcatgc tcgggcgtga actcggggac cgtgtagggc gctccgggca
     6421 gatggcggat gacccatgag taatacatgc cgtagccggg cgccgcgcat tgcgcatggt
     6481 cgtagcccgc cggtatgcac accgagtcga actggcgcac cttgacgact tcgtcgccga
     6541 gctcggcgtg gccgtagccc tgcggggccg tgaaccggta atggtagatc tcgggctgcg
     6601 ggtggtgatg cggcggatag ctcgaccagc ctcctggaaa ggtcaccact tcgccgagca
     6661 cgagcccggc tgcgggcggt gagtttctgc catcgaagat cgtgcgcacg aagcgcaggc
     6721 tgcggtcgcg gacctggccc ttgccgcgat gctcgttcgg cacgtcggcc ggacggaaga
     6781 tgcgcggcgg aaaacgggcg gtgttggcgc agctgtagac ggtcagttcg cagtcgcttt
     6841 ccgcctgcag cgtgacgcgc gtgccggccg agacgtgcag gcagctcgcc gattcgtcga
     6901 atagcgagcg gcgcgtgagc acggccgctt cctcgcaggc gcgcacgcgc acgctgcccg
     6961 acatcagcag ccaggcggtt tcgccgtcca ccgtgtccga ccaggattga ccggcggcga
     7021 ggatcatgac ggcgaggccc agtccggtgg cgttgtcggg atcggctgca cgcgtgatgg
     7081 ccgtgaggcc ggcgggaaat ccctgcctgt gcgtgctgat gtgattcatg ccggtatctc
     7141 cgtccagctg cgcgtggcgg ccgactgctc gatcgcttcc atgacgcgct gtacgcgtgc
     7201 gccctcgtgg aaatcgggcc aggcgcgggc ttcgccgtcg agcgcacgca gcaggttgcg
     7261 tacctcgatc actttcaggt cgttgtagcc gaggccgtgg ccgggcgccg ggcagaacgg
     7321 gcggtaatcg gggtgttcgg gccccgcgag gatggtgcga aagccgctgc gcgccgccgg
     7381 gccggccgcg tcgtaccagc gcagctcgtt catgcgctcc tggtcgaatt cgatcgtgcc
     7441 tctggtgccg aagaccgtgc aggcgagccc gcacttgtag cccgtggcga cgcggctgat
     7501 ctcgaagctg ccgggcacgc cgccctcgaa ctccaccagc gcgcgcgcga tgtcctcgtt
     7561 ctcgacggct cgccactcgc cgctcgccgg gtcgcgccgc gtggcatgca cgagctgcag
     7621 gctgccctga acgcgcgcta taccgccgag cagcaggtgc ccgaggttga tcaggtgcga
     7681 gccgagatcg gcgagcgccc cgctgccggc cagctcgcgc ttgcagcgcc aggtccacgg
     7741 cgcggccggg tcgctcatgt agtcctcgaa gtagctgccg cgaaacgcgt agacctcgcc
     7801 gagttcgccg gcggcgatca tgtcgcgcgc cacctgcaac agcggattgc agacgtagtt
     7861 gaagcccatc atgtgcgtca cgccggcggc gtcggcagcg gctgcgatct cgtgcgcctc
     7921 gcgcgcagtg agggcgagcg gcttctcgca gtacacgtgt ttgccggcgg caatggccgc
     7981 gagcgccatc tcgcgatgca ggaagttcgg tgtgcagatg tcgacgacgt cgacggcggg
     8041 atcggcgacg acgtcgcgcc agtcgccggt ggatcgcgca aatccgagcg cccgggccgc
     8101 ctgcgccgct gcctcgggcg aggagtcggc gagcagctcg cacaccggcg cctcacgctc
     8161 gacgaacacc gcgccgacgg cattcagcgc gatggcgtgc gcgcggccga tgaagccggt
     8221 accgatcaat ccgaatctgc ggcgagtcat gggtatgcgg gggtcagtcc agggttgaaa
     8281 ccggagcgcg cagttcgagc gagcgttgtg cggcgtcggc gaggcgcagc gcctgcctgc
     8341 cgtcgctgcc ggtcgcgagt tgcgccggct cgccgcgcag cgccttcagg aactcgtcga
     8401 gctcgtcgcg ataggcggcc gcgtagcgct cgaggaagaa gggcagtgcc ggctcgcgcg
     8461 tgaagcctga ggcgtcggcc gtttcgacgg tggtcgcggg cacgttgtcg gcgcgcacca
     8521 ggccggccga gccgaagact tcgatgcgct ggtcgtagcc gaaggcgcag cggcggctgt
     8581 tcgtgatctg gcacagtcgc ccgctggcgg tctcgagcac gaccaccgcg gtgtcgatgt
     8641 cgccggcggc gccgattgcg gggtcgatga gcgcgctgcc gcgcgcgtag acgctcaccg
     8701 gctcctcggc gagcagccag cgggccatgt ccaggtcgtg aatcatcatg tcgcggaaga
     8761 ggcccccgga gcgggccaca taggcgggcg gtggtggcga cggatcgcga ctcgtgatgc
     8821 tgacgacctc gacgttgccg acgcgaccgg cggcgagctc gcgctgcagg cggcgaaatg
     8881 ccgggtcgta gcggcggttg aagccgagcg cgagcagcgt ctttgcctcg gcgaccaccg
     8941 ccaggctcgc ctcggcgcgc gcgatatcga gatcgagcgg cttttcgcag aacaccgcct
     9001 tgccggcccg tgcgccggcc tggatcagct cggcatgggt gtcggtggag ctcgcgatca
     9061 ggatcgcgtg caccgccggg tcggcgagcg cggcgcgggc gtcactgacc tgcgcgccgc
     9121 tcgcgccggc gagccgccgg gccgcctccg cggagacgtc gacgacatgg cgcagctgcg
     9181 cgtcggcgtg cgccgcgagg ttgcgcgcgt ggatctggcc gatgcggccg gcaccgaaca
     9241 gcgcgaatcc gtacttcatc gcgatttgcc cttgctggca cggcgctcgg actggaacgc
     9301 gtagctgacg accagcgcct gcgcgaggca cagcgacgcc gtcagcgagc ggaactggag
     9361 cacctcggcg tccttggtct cgaagaccac gtcggcgtcg cgcgcgatcg gcgagacgcg
     9421 cgtgtcgctg atcgcgatga tgcgcgcgcc gcgagcgcgc gcctgcgcga cgatctcggc
     9481 cgtttccgcc gcatagggcc ggaagctgat tgcgatcagc aggtcgttct gccgcgccat
     9541 gccgctctgc tcggcgagca tgccggcgac gccgtcgagc aggtaggcgc gcttctcgtc
     9601 gacatggcgc aacgcgtagg cgaggtagga ggccaccggg aacgagcggc gcagtccgat
     9661 gatgtagacg gccgcggcct cgcggatcag cgcgacagcc ttttcgaggt cggccttgcg
     9721 gatcgagccc ttcaggtgtt cgagggcgat gatgttggcg tcggcgaact cctggagcaa
     9781 ttcctgcggc gcgagcacgt cgaccgcgcc cgaccggtcg tcgaactgcc ggatccggtc
     9841 ggcatagctc gggctcggcg cgaaggtcag cagttcgtcg cggaacagct tctgcatgtc
     9901 gctcgcgccc tcgtagccca tcgccttcgc gaagcgcacg acggtggaag gctgcacgtt
     9961 gcagcgctcg gcgatcaccg cgagggtctg cagtcccatg tcgttcgggt gatcgagcgc
    10021 gaactgcgcg acctgctgca ggcggggact gagttcctca tagcggcgca cgatgtcggc
    10081 gcgtagtcgt tcgaggtctt ttggggcggc cacgggcgtc atcctgatga aatagacact
    10141 tgcaacactt cccggcaccc gggcgtggaa agaattagaa catatatttc atcaattgcc
    10201 acaggcaaaa ttctcgttct atactctcgt cctgatggaa cccggattcc atggcacgag
    10261 ggggcggctg ctctcccgcg ggtggcgatc gtgaaacaac gacagtgtca ggacaccatt
    10321 catatgaagc ttcgccaatc gtctctcgtt gccgcggcca tcgccgcggc cctggccggc
    10381 catgccggca acagcctcgc cggtgaactc gccggccagg tggcttcctc ggccggcgag
    10441 tcgctgccga acgccaccgt gcgcattccc gaactcaatc gttccgtgcg ggccgaccgc
    10501 tcgggcgcgt tccgcttcac ggacgtgccg gcgggcgtct acaccgtcgt cgccgagtac
    10561 gtgggcttcg gccaggcgag cggcagcgtg acggtggccg agacgggcgt tgcgacccag
    10621 aacttcctgc tgaccggctc gaacatcgag gaagtcaccg tgaccggctt ccgcctcgcg
    10681 caggcgaccg cgctgcagga caagaagtcg gcgaagtaca tcaaggagtc ggtcacggcg
    10741 gacgacgccg gcaagctgcc ggatcgcaat gccggcgatg cgctggcgcg cgtgaccggc
    10801 gtgtcggtga ccaccgacca gggcgagggc cgctacgtca acatccgcgg catcgacgcc
    10861 gcgctcagca atgtcacgat cgacgggcag acgatcggtt cgccggaagg cgacacgcgc
    10921 cgcatcgccc tcgacaccgt gccctcgaac atcctcgcga agctcgaggt gatcaagtcg
    10981 gtgacccccg acctcgacgg caacgcgatc ggcggcaccg tcaacctggt cacgccgagc
    11041 gccttcgacg acccgaaggg ccgctccttc gcgatgtcgc tcgactacgg ctattcggac
    11101 ctgggtggcg agaatccgtg gggcgcaagc gccgcctgga gcggggtgtt cgccgacgat
    11161 cgcctgggag tcatcctgtc ggcgagctat tcggaccgcg aattcaactc gcagaatatg
    11221 cagggcgggg acccctggca ggaggagggc gacttcctcg tgcccgacga actgaacctg
    11281 cgcgactacg taatccggcg cgtgcgcaag ggcctggtcg cgaacttcga gttccgtccg
    11341 aatgacgcga cgaagctgca ctggcgcaac atcatcaatc gctatgaaga cacggaactg
    11401 cagcccgaag tcacctacga ttttcgcaac ggcgatctcg aggaccagac agcgacctcg
    11461 ggcctgttca ccgaaggcga gggcgagcgt gcgaactccg agcgcttcga agtccaggaa
    11521 ctgctgtcct cgacgcttgg cggcgagttc gaactcggca actggaacct gaacgtcgtg
    11581 ctaacctacg gcgagagcga gcaggacacg ccctacgaca atttctacgc cttcgaatcc
    11641 gccgacgaga tgccgatgac gtacgacacg tcggaccgct tctggcaggt cggcgcgccg
    11701 gaggaattct acgacgccgg gagtttcgag ttctccgagg ccgcccgcgg cgggcagctg
    11761 atcaaggagg agctgaacat cgcccagctc gacctgctgc gcaatgtcga gtggggcggc
    11821 ggaaactccg gctacatcaa gttcggcggc aagttcgcga gccgcgagag cacttccgac
    11881 caggacatga tcgtctatga cggcttcgac ggcgacctgc tgctcacgca ggtcgccgcc
    11941 gcgggtgacc cgggcttcta ccgggacgtg cggccgtact accgcttcgg accttacccg
    12001 gactacggcg cgtccgagga tttcttccgc gccaacgagg gactcttcga ggtgagcgac
    12061 gcggatacga gcatcgagtc ctacggcgtc gactacacgg tcaaggaaga tgtgacggcc
    12121 ggttacatca tgggttccgc cgacatcggc aatgtgaccc tggtcggtgg cgtgcgcgtc
    12181 gagcgcaccg atacggactt cacggcgttc gatgtgcaga tcgtcgacgg cgatgcgctc
    12241 gacccgccac cgcaggtgat cggctcgaag gactacacca actggatgcc cggactgcag
    12301 gcgacctggg ccatccgacc cgacctgatt gcgcgcgcgg cctggaccaa cacgatcggc
    12361 cgcccgtcct acgaacagaa cgtgccgttc cgcatattcg aaaccgagga ggacgatccg
    12421 ggcatcttcg aaggcgccat cgagatgggc aatgccgacc tcgatccgct cgagtcggcc
    12481 aattacgacc tggccatcga gtggtacctg cggccttccg gcctgctatc ggccggcgtg
    12541 ttcttcaagg acatcgacaa tccgatcttc acgcgggtgc tgacgctaga ggatcaggac
    12601 ttcgagggcc gcttctactc cgagctcgag gtcgtgcaac cgcagaacgc gcgcagcggc
    12661 gacatcttcg gcatcgaact cgcctaccag cagcagttca gcatgctgcc cgggttcctg
    12721 cgcggcttcg gcatctcgat cggctacacc tacaccgaca gcgaggccga gaccgcggat
    12781 cgcacggaaa aggtgccgtt cttcctgcag tccgaccatg tcggcaatgt cgctctcttc
    12841 tatgagctct ccggcctcga gctgcgcctc ggttacgcct accgcagcga gtacctggtc
    12901 gaactcggcg acagtgccgc cgaggacctc tacatcgaca cgcacggcca gctcgacttc
    12961 aaggcgagct accaggtgac cagcaagctc ggggcgttcg tcgagatgaa gaacatcacc
    13021 gacgagccgc tgcgctactt cagcggcgac cgctcgcgga tggcggaaaa cgagttctac
    13081 tcgtggacga tcctcgccgg ggtgaacctc aagttctagc cggcagattc cggcggcggc
    13141 gatgccggga ccgtgtaggc tcccggcatg cccaaccgcc cgctgcgcgg catcgcgttc
    13201 atgactgccg ccgtcgccgt gtttgccgtc atggacgcgc tgctcaaggc gctgacggcg
    13261 cgctatccgc cgctgcaggt ggccgcgctg cgcggcgcgg cgtcgctgcc cttcatcgcc
    13321 ttgccgctgc tcctctcagg tcgcgccgcg accctgatgc cgcggcggcc cggcatgcac
    13381 ctgctgcgcg gcctgctgat ggtcgtggtc atgggcggtt tcctgtacgc cgtgcgctcg
    13441 ctgtcgctgg ccgacgccta ttcgatcttc ctgaccgcgc cgctgatggt caccgcgctc
    13501 gccgcccttg tcctgcacga gcacgtcggc tggcggcgct ggctggcgat cctgatcggc
    13561 ctcggcggcg cactggtcat gctgcgcccg tcggcgtcgg gcttcgcgac cgtggccgcg
    13621 atcggtgcgc tggtggcggc gctcggctat gcctgcaacg cgatcctgct gcgtgtgctg
    13681 acgcgcaccg acaccaccgc gagcgtggcg ttgtggtcga tcgcagtgat gaccgtggtc
    13741 gcggcggcga tcgcgctgcc ggcctgggtg acggtagggc gtacggacat ctggttgatc
    13801 atcgcgctcg gggtcgtcgg ggcggtcgcg cagatgtgca tgatcgaggc gtttcgtgcc
    13861 gcgccggcct cggtgatcgc gccgttcgag tattcggcgc tcttctgggg catctgcatc
    13921 gactgggtgg tgtggcaggc attgccgacg ctgcgcgtgc tcgcgggcgg cggcatcgtg
    13981 atcggctgcg ggctctacat catctggcgc gagcgcgagc tcaccatgcg acgctgacac
    14041 ccttggtctt gaggtaggcc tcgagcccct ccttgccgaa ctcgcgtccc cagcccgact
    14101 gcttgttgcc gccgaacggc atcgccgggt cgagtgcggc gtgcatgttg acactcacct
    14161 ggccggagtc gatccggcgc accatctgca gcgcgcggcc gatgtcgcgc gtccagacgc
    14221 tgccggaaag gccgtagatc gtgtcgttcg cctcgttcgc gattcgctcg atctcgtcgt
    14281 cgccgaacga ttgcacgcag agcaccggac cgaagatctc gttcatcacc ggcgtcatgc
    14341 cggcgtgcat gcgggtgaat atcgtcggct cgaagaacca gccgctgcgc gccggcgcgc
    14401 tgccgccgca tacgagttcg ccgccttcgc gcgtgccggc ttcgacgaag ccgcgcacgc
    14461 ggtcgcgctg ttcggcggaa atgagcggtc cgaactgcga cgccgggtcg aggccatggc
    14521 cgaggcgaat gccgcgcgcg atcgccgcca cgccgccgac cacctcgtcg tggaccttct
    14581 cgtgcacgaa caggcgcgtg ccggccatgc agttctgccc ctgcaggaag aagcaggcgc
    14641 ggaacgcacc cggtatcacc tgctcgaggt cggcgtccgg gaacacgatg accggggatt
    14701 tgccgccgag ctcgagcgac accttcttca ggttgccggc cgccgccctg acgatcatcc
    14761 ggccgacctc ggtcgatccg gtgaaggcga tcttgtcgac gcccggatgc gcggcgagcg
    14821 ccgcgccggc ggtttcgccg agaccggtca ccacgttgac cacgccatcg ggcacgcccg
    14881 cctcggcgca cagctcggcg atgcgcagcg cggtgagcgg cgtgtgctct gcgggcttca
    14941 gcacgatcgt gcagccggtc gcgagcaccg gtgcgagctt caccatcagc atcggcagcg
    15001 gcgcattcca tggcgtgatc gcgccgacga caccgatcgg ctcgcgcagc gtgaacgaca
    15061 gcacctcgcc cggccggcgt ccctgcggcg cggccgggat cgtcgcgccc tcgatcttgg
    15121 tcggccagcc cgcgtagtag cggaagaatt ccggcgcgag gcggttgacg acgagttgcg
    15181 cgatcgcgag cggctggccg ttgtcgagca cctccagctc ggtgaggagc ggcgcgtcgc
    15241 gctcgatcag gtcggcgagc ttccacagta cgttgccgcg ctcggcggcc gtgaacctgg
    15301 cccacggccc ggtgaacgct gcgcgtgcgg cggcgaccgc cgcgtcgatg tccggcgctt
    15361 cgcccgccgc ggcggtggcg atcttcccgc cggtcgccgg gtcgtagacg tcgagtctcc
    15421 cgccggcgag cgggttcacc cacttgccat cgatgaagag ctggtgcgtg cgcgcaagga
    15481 acgcctgggt ggcggcggcc ggcgccggac tctggattgc tggcatgacg cccatgctac
    15541 cgccccgagg tcggcgcggc atacctgcat cccgggcaat gcccgacacg tggcggcgag
    15601 cgattccggc cctgtgcttc gggctgccgg ctcagtgcgc acccgccgcg accgacgcgg
    15661 tggcgagatg cgtgtcgccg ggcgcggctg gagtgggcac ataccggtgc tcgcggaaca
    15721 tctcgtacgc ggtctggtaa tacgcaattg ccagcgcgtc gagccgacgg tcggcgggcg
    15781 ccggggcgta tgcatcgttc gcggcgtcgt aactcaggtt ggttacggcc tttttcaggc
    15841 cgaggatcgc gagttcgtgc tcacgcagca gcgctacgtc gtggttgtgg ctgaagagcg
    15901 cgatgggctc gtcggtcggc ggggcatggc gcacgtcctg gccgaagaac ggcgcgcggt
    15961 agggcaggcc gagcaggccg agcacggtgg gagcgacgtc gatctgcgtg gtcaatgtgt
    16021 cgacgcgctc cggcgcgata tgcttcggcg cgtagaccag catcgggatg cggtaggtcc
    16081 tgagcggaat atcctcccgg ccatatacgc gcgcgccgtg gtcggcgacg atcacgaaca
    16141 ccgtgtcgtc gaaccagcgg tgcgcggccg cctcgcgcag gaagtatgcc tgcgcgtagt
    16201 ctgcatagcg cacgcccgat tcgcggccgc cgccctctgc cttcacgccg ggcacgccgg
    16261 ggcgaaacgt gaacggcttg tggttcgacg tgttcatgac gatcgcgaag aacggcttgc
    16321 cggtgtccgc gagcgcatcg aagcggcgca tcgcgaggtc atagagatcc tcgtcggcga
    16381 cgccccagat gttctcgaag cgcactggcg tgtcgatctc gcgccggtcg gccacctcga
    16441 aaccgttgcc ggcgaagaac gcgttcatgc cgtcgaagct gccatagccg ccgtagaaga
    16501 agctcgtgct gtagccgagc tcgcgcatga ccgctcccca attggcgatg ccggtgttgc
    16561 ccggccggtg caggatcgag gtcgtcggga tcggcgggat cgaggcggtg atcgcctcga
    16621 gtccgcgcac cgtgcgcgtg cccgaggcgt acatgttcgt gaacatgatg ccctggcgcg
    16681 cgtactcatc aaaatatggc gtccagtcgt gctggccgcc gaatgcgcgg ctgaactccg
    16741 cgccgaaaga ttcgctgacg atcacgacga cgttgagccg gccgagtccg tcggggcggg
    16801 ccgggaaggt gcgatcgatg ccgcgatcgt cgaccagccg cccgccaccg cgcgcgaggg
    16861 tcgtgcgcag cgcatccaga ttcgcggcgc ggtccgccgt cgcgtagtag gcgtgatagt
    16921 cgatctcgct ggtgcgcagc gcattgaaga agctggccgc gccgttcgcc gcgatctcgt
    16981 tggtgacgcg attggtcgag aaggagaagc tgtcccggtc gatcgcgacg accgcggtgg
    17041 cgaccagcag cgcgtagacg agcgccgtcg ccgagcgcgc gccgaacccg gacgcggcgc
    17101 gcgtcccggc gagcagcggc cggcggagca catacgcggt ggcggccgcg agcagtgcga
    17161 ccacgagcac tatcgggatc accgggtatt cgctccggat gtcaccgacg acctcggtcg
    17221 ggtacatcag gtagtcgacc gacaccaggt tcagccgcga gtcgaattcc tcgaagaagt
    17281 agtactcggc gaccgcgacg aagcccatct ggaagacgaa cagcgcggcg agcgcgagga
    17341 tcgtgacgcg catgccgcgc gtgcggaacc agcgatcggg cgcgagccag gcgagcagcg
    17401 cgaaaggcgc gagcagatag agtgcctgca cgatgtcggc cgccccgccg gccgccagcg
    17461 tgccgacgaa ggcgacgtcc gatacctcgg ccgggcgccc aaacgaccac cacagcgcgg
    17521 cacgcaacag cagtccgacg acgagatacc acgcgagcag cgtggtcagg agccggaatc
    17581 tcggccaggc ggagcgcagc atgttcagga attgcgtcat gttcagaaac tcttgtgcgt
    17641 cttcaggagg aactgcatcg agtgcagcga cgggtcaccg tccagcggaa aggcgagatc
    17701 gacgtgcagc acactaccga gcgccgagcg cgtgttgccg aagcgcaggc cgatgccgac
    17761 gtcgcgcagc gtgccgagtg cgggcacgtc gagcgcgctc ggcccccagg tgcgaccgac
    17821 gtccgcgaac accgccgcgc cgacgcgcgc cagtcggaac ggataccagt cggtgaagta
    17881 gcgtgcctcg atcgtcgcga gtgcgcgccc ggtgccggtc tggtaacgca gtggatagcc
    17941 gcgcacgccg gtgtcaccgc cgagctgcag ttcgtgatcg gcatcgagcg ctgcgccgtg
    18001 gttggcggcg aggccgacga acagtacggt gcgctcaccg gtgcgccaga agtagcgcgc
    18061 ctcggccgac agcagcgagt cgcgcaactg gcccgcttcg agtcgcccgt tccagccggc
    18121 cgagagaaac aggttctgcc ccggtcccag ccggtaaccg cggctcgcac cgagactcgc
    18181 gatgaacgca ttgcggtcgg cgccgaacgc cgactgcgac cagccgagtt ccgcggtgag
    18241 ccgcgtaccg aagcgcagat cctcggtgcg cccgatctgt tcgagattgc gggccgtcac
    18301 gaaatcgtcc tgcagcagct cgaagcgcgc gaatggatac gcgagtgtcc ggtcggcagg
    18361 caggggcgcg agcaacgccg tgtccggcag cgattcgaac tccacgctgt cgtagcgata
    18421 gccggcgctc cagcggcgca cccagccgtc ccgcaggccg gatgaccagc cgcggtgaac
    18481 ttcgaaccgg cgcgcgtgcg agcgataggc gtcggcgcgc tcgccgaggg aatagcgcgg
    18541 atcgatctgc gtgccggcgg ctgccgagaa ccccgcgctc cagcgcgcgt cgagcgcgta
    18601 gaagggacga tcgacgccga gcgcccagaa ccggccgtcg tcactgccgg tgtagccggc
    18661 atcactggtc cagcgcgagc cgaagaccgc ggggtcgtgc catgtgacgc cgatggaagt
    18721 gcgatcgacg tcgctcgtgt agccggccga cagctgcttg ccccggccga acaggttcag
    18781 ctcctcgatc tcggcgccga ggcggttcgc gccgccgctg cgcgacaccg acaccccggg
    18841 gttggtcgtc cagacatcgc tcgcgcggac ccgcacatcc accacgccgt cgtgccaggc
    18901 gaccggccgg atcgcaggct cgtgcaggta gcgctggcgg cgcagcacac gctccgactc
    18961 cgcgagcagc cgtcgtgaat aagggtcgcc ggaggcgaac agcagctgcg cacggatcgt
    19021 cgcctcccgg gtctcgacgt gcagccggtt ggccgtgcga tacagcaggg aatcctcgcg
    19081 cgggtcgttc tcgtcgaaga tgctctcgca gtcgatctcg atcgcgccga tgcgcgcgcc
    19141 ggcgcgctcg aggacctcgt cgggcgggat ggcgacggga tcgggcgacc cggccgcgat
    19201 tcgtgaggcg gcgagcagga cgattatgaa ggcggcggtc cgcatgcctc tggttcgacg
    19261 gtccggcggg tgcgaatcaa ttgcgattga tcttcgggcc gaggcctgtc taaccttgtg
    19321 ccgatgcaac aggacgaccg tgccctcgtg gcgcgactgc tggccggcga cgacgatgca
    19381 tttcgaagtt tcttcgacga gtacttcgac cggctgtacc ggtttgcgct gtcgcgaact
    19441 tccggtcgaa ccgaccttgc ggaagaggcc gcgcaacgca cgctgtgccg ggcgctgcgc
    19501 gcattgcagc aataccgggg tgaggcttcc ctgttcacct ggctcgcgca gatctgccgc
    19561 aacgagctcg cggatctggg cgaatccgag cggcgggatg ccgagcgcca gcagagctac
    19621 gacgcgagcg agcgcgcccg ccagctcgcg gagggcgtgg cgagcgccga cgcggctccg
    19681 gccgagcggg cgcaggccgc cgttgcgtcg gacatcctca ggcgcgcgct cgatgcgctg
    19741 ccggggcgtt atggagacat actcgaatgg aaatacctcg aggactggag tgtccagcag
    19801 atcgcggcgg aactcggtgc gagcttcgag tcggcgcaat cctcgctcgc gcgcgcccgc
    19861 cttgcgctgc gctcggcgct cgtcgcgagc ggcatcgact ggcatgagct gctgccatga
    19921 ctgaaagaac gggcgactgg accacggccg acgaggcacg ccttgcgcgc ctgatgcgtt
    19981 cactgccgcg cggcacaccc gatcctgcgg cgcgggcgcg tgctttcgag gccgcgcagg
    20041 ccgaatggcg gcgcagtcgc gcccgcccgc tgcgcggtgc gcccgtgcgg ctcgcgggct
    20101 gggccgcggc cgtcgcggtg ctcgcgatcg gggttgcgct gtgggtgcag ccctatgccg
    20161 cgcccgtcgc gcatctcgat cgtgtttacg gcagcgtgtc ggcagatgcc gatccgctcg
    20221 aggtgggcgc gcggctgcgg cgcggcgcgg ccgtcgagac tgcgcaggaa tcgggcgcgc
    20281 tgctgcgcta ttcgccggac ctgaccgtgc gcctcgatgc cggcacgcgc gtcgcgatgg
    20341 tcgatcgcgc gacgctgcgg ctcgacgccg gccgcgtcta catcgccgtc gcgccaggga
    20401 cggcgggggc tttcgtggtg cgcacggccc tcggtgacgt gcgccatgtc ggcacgcgct
    20461 acgccgtcag cgcgcgcgac ggcgagctcg aggttgcggt gcgcgagggc gaagtccgga
    20521 tcgacctggg cgcacgcgcc gagcgcgcca ccgccggcga ggcattgcgt atcgacccgg
    20581 agggcgcgat cctgcgtgcc gtactgtctg ccgacgatgc gcggtgggct tggatcgaca
    20641 gcttgccgtc cccggtcgcg atcgaaggcc ggacactggt cgagtttctg caatggtacg
    20701 ccgtcgagac cggccgtcgg gtggactatg ccgacgatgc gacccgcgcg cgtgcacagg
    20761 cgacgatcct gcacggctcc gtcgatgggc tggcccccgc gcaggcgctc gcgatcgtcg
    20821 cgtcttctgc cgatgtcgaa gcgatcctgc gggacggtca gctcgtgatc ggcccggcgc
    20881 gtcgctgacc cgcagctgct aacattcctg catgccaggc actctgcgga cccgggcgcc
    20941 cgcctggagc actctcctgt tgctgttcgc gagcgcattg gccgccggcg tcgaggtggg
    21001 tgaaccgctc ggcgccgcgc tcgacaggct cgcgggttcc ggactcaggc tcgtctacag
    21061 cacggcgctg gtcacgcccg atctgcgcgt gcgtgcggtg ccgcgcccgg gcccatctgt
    21121 cgagatggcg cgcgaactgc tcgcccagca tggcctcgcg ctcgagccgg tgcagccagg
    21181 cgtttacgtc gtcgtgcgcg ccgccgccgg cgacgcgacg gcgggcggca gcgtcgcggg
    21241 gcggggcggt gcgccggtcg gcgatgccgc cgcaccgctc atcgaagtga gcattctcgc
    21301 cagccgctac cgcatcgatc agcagtccga attcgcgccg gtcgaaatca cgggtgagga
    21361 tctggcggcg cggcccggcg tcgacgcgga tgcgcttcgg gtcacgcgcc tgctgccggg
    21421 cacggcgacc aacggtctgt cggcgcgtgc ctacgtgcgc ggcgggcgcg cggacgaact
    21481 gggcgtctgg ttcgacggcg tgccgctgtt cgagccgttc cacttcaagg atttccaggg
    21541 cctgcttggc atcctcgatc cggcgaccgt cggcaggctg gatttctgga gcggagtgca
    21601 tccggcgcgc ttcggcgggc ggctctccgg cgtactcgac atcgcgccgc gcgcgtggaa
    21661 cgacgagaac caccatgaga tcggcgcgag cctgctgtac gcgcacgcgc tgtcgcaggg
    21721 ccggctcgag gcagcgccgc tcgaatggct cgtcgccgtg cgcaagagca cgatcgacca
    21781 gatcatgaag ctcgccgagg agcgcaccgg cgagcccgaa ttcctcgacc tgctggtgcg
    21841 cctcgcgtgg cggttcgatg gcggcagcga cgtcgcggcg ggctggctcg tgctggacga
    21901 cggactgacg gcgaacgcct cggacgacac cgagcgcgcg cgggcgcgct accgggacgg
    21961 cactggctgg ctgcgcggta cctggcgcat cgatgatgac ctcgaagtgc gcggtctcgt
    22021 ctcgcgcacc gagcggcaca cgaggcgtgt cggcacggtc gatcgtgcgg gcagcgccac
    22081 gggggcgctc gaggaccggc gcttcatgga tgcgaacacg gcccgcctcg aactcggcgg
    22141 tcgccacgga cgcgtgcggt atacgctcgg cggcgagctg atggatcacg acacctctta
    22201 cgagtatgcg gccaatgccg tgttcgatcc gctgctcgcc gccgcgctcg ggcggccgga
    22261 tgcgctgagt cacgatctca cgctcgtggc cggcggccag tcttacgcgg cctatgcctc
    22321 ggcgctgatc ggcctgtcgc cacggctcga cgtcgacctg ggcgtgcgct gggacaggca
    22381 ggattaccgg ccgggcttcg atgattcgca gtggtcgccg cgagtcggta tcgagtatcg
    22441 gccgcgggag ggcagtgcgc tgcgagtgtc cttcggccat ctgtcgcagc ccgagcggcc
    22501 ggacgaactg caggtgaccg acggtgatcc gcagttccac gccgtgcaga ccgcgaccca
    22561 ggccgtcgtg gcgctcgagc atcgcctcgc gaaggaccag cgcatcaggg ccgaagtgtt
    22621 cgacaagcgc atgagccacg tgcggccggt ccacgagaac attctcgatc ccgtgacgct
    22681 gttgcccgag atcgaggtcg atcgcgtgcc tgtcgcgccg cgctcgtcac gcgcctacgg
    22741 cgcggagttg tcgtggtatt gggagccgcg cgggccatgg tcggcgtcgc tcggttacag
    22801 ctggtcggaa gtgaccgatc acttcgacga ttcgcgcgcg ctgcgcagct gggaccagcg
    22861 gcatgcggct gtcgccggcc tcacctggac acgggacccg tggcaactgt cgggaagcct
    22921 cttctggcac agcggctggc gtcgcaatga cctgcgggtc gaagtctcgc ccgagggcga
    22981 ggcgcagctc gtgctctctc cgcgcaacgc gggcacctgg ccggattact tatcgctcga
    23041 cctgcgggcg agctggcagc ggccgctgcc ggtcggcacg ctgcaggtgt tcgccgagat
    23101 cagcaatctc gccaataacg acaactactg ctgtaccgag taccacctcg ccgtgcccgg
    23161 cgacgcggcg agcctcgagg gcgaacgctc gacctggctg ccgcgctacg gcctcatcgg
    23221 ggtgaagtgg atcctgccct gagaggttac ggcggcgcgt agcgaatcgc gcgcgaggca
    23281 tcgccgcgcg cgacacagtc cggtgccggc gagcccgtga cgagcgacca gcgctcgccc
    23341 gcactggtgc gtgcgccgtg cgcggtcgcg gtggtgaagc acggtgcgag cagcgcatcc
    23401 ggcacgagca gcacccggcc cggcgcggca gcgagccagc gcgcggcgtc tgcctcctcc
    23461 gcgccgtcct cgcgccagcg gcggtgtccg aagttgacga tcggacgatc gaggtgcaac
    23521 aggaactgct ccttataggc gaccaggccg agttcacgat ccggcggcac ctgggcgagc
    23581 acgtgctgca tgaatgcacg accggagcgg atcgggtcga tcgccggcgc gatcgcccag
    23641 gagaacgagg cggccagcgc ggcgagcacg agcggccagc ccaggagggg gcggcgccat
    23701 gcgaggagcc acgcagccgc gcaggcgaag gcgggcacgt acatcggcag atcgggtggc
    23761 aggttctccg ctgacagcag cgtgtgcgcc tgcggcagat cgagcgccac ggcgatcgcg
    23821 atgccggcga cggcgacgag cagcacaccg gacagcgcga ggctcgcgac ggcgacgcca
    23881 cggcgcgcga gcactgccgg cagcaagggc gcggcagcga gcgcgaggcc cggcaacgcc
    23941 ggcagcaggt aaacgccgcg cttcccgggg ctcagcgtga agaacagcag cacgagcgcg
    24001 acccacgaca gcggcagcca gatgcgcgcg tcgcgcgcac gccaggcggc gcgccacccg
    24061 ggtaccagcc agaacgcgag tatcgaccag ggcaaccaca gcgccggtat gacctcgacg
    24121 aggtagtagt accaagcttt cacgtggtgc cacgacgccc cgtaacgcgt cagggtctgg
    24181 tgaaagagga tctcgtcgcg ataggcggcg aattcgggcg tcccgtgccg ggccaccgcc
    24241 cagaccatcg gcacgagcca cagcgcgatc gcggcgagca ggccgagcgg cgcgagcgcc
    24301 cagcgccagc cgccggcggc tgacggcgcg agaccttgcc agcgccggcg ccgcaatatg
    24361 gcgtacggaa tcaagagcag caacggcagg aagccgacac ccttcgtgat cacgccgagc
    24421 cccgcggcga agccgccggc gaagtaccag ccccacgcgg gtccgagcag catgtggcgc
    24481 gtgagtccgt agcatccgag cgtgatcagg aacgcgaccg tcgcatcgat ctgcgccgcg
    24541 cgcccctgga gcacgaattg cagcgtgcag gcgagcgtga acgccgcggc cagcgcagcc
    24601 tcgcgcccgg cgagccgtcg cgcaagatcg tacacgagag ctacggtggc gcaggcggcc
    24661 agcagcgagg gcagcaggaa ggcgatgcgc agcgagcccg tcagcgacag gccgagggcg
    24721 atcatccaca ggtagagcgg tggcttgtcc ggatagagct cgccaccgac gcgcgggaac
    24781 agccagtcgc cgctcgcgac catgtcgcgc gcgatcagcg cgaagcgggg ctcatcggcc
    24841 ggccacggat cacgtatgcc gaggccggtc gcgagcagcg cgagcagcgc gagcgccagc
    24901 cacgcgtagt gctgcaggtc ctcagcgcgc cgcacgcagc aactgcaggt tgcgaacgta
    24961 gacgatcagg cccgcgccct ggccgacgat gaataccggg tcgcgccggt ggatcgcgta
    25021 ggcgagcagc aacgcgcctc ccgcgacgct gaagtaccag aaggcgtgcg gcacgatgct
    25081 gcgcccgcga cgctcgctcg cgatccactg cacgaagaag cgcgccgaga acagggcctg
    25141 cgcgacgaag ccgatcacga tccagacggt ctcatgggtc atcgcggttc cccggtgagc
    25201 tcccgcacct cgaggcgggc ccaggtacgg tgcgcgagcc acatcacgcc atacagatcg
    25261 acgatgccga cccacagccg gttcagcatg ccgtacttcg agcgcccgtg ccggcgctcg
    25321 cggtgcccga ccggcaccga cacgacctca ccgccggcgt gtcgcacgag ggccggcagg
    25381 aagcggtgca tgtggtcgaa ccggggcaga tcgaggaaca gtgcgcggtc caaggccttg
    25441 aggccacagc cggtgtcggg agtgccgtcg ccgagcagcc gtgaccgcac gacattcgcg
    25501 atgcgcgatt gcaggcgccg ccaggccgta tcccgccggc tctgacggtg gccgatcacg
    25561 agcagcgggc gatcgttgcc cgcccgctca tgcgtcgcga gcagccgcgg gatatccacc
    25621 gggtcgttct gcccgtcgcc gtcgagtgtc acgatgcgcc tgccgcgcgc ccggcgtacg
    25681 ccggtgcaca gtgccgcgct ctggccggcg cggcgtgaat ggcgcagcag gcgcagcggc
    25741 gcgacacccg ccgcggcgag ccgcagcaca cgctcgcccg tgccgtcgct gctgccatcg
    25801 tccacgaaga tgatctcgta gtcgcggccg gcgagcgccg tggcgatctc gccggccagt
    25861 ggctcgacgt tctcgacctc gtcgcacacc gggacgacga ctgacacttc gacctggttg
    25921 ctcaaggcat cctccggcca attgatgttg gacggcgcgg gcaggatcga tcaatcggtg
    25981 ccggcaaggc gtgcgcgcct gcggcatact cggctcatgt ccaaccgccc gagcacgcgc
    26041 tccgctggat gaaccggacg cgggtgaacg cgccgttcct gcgccgtcat gtctggtggc
    26101 cgctcgcagc atatgtcgcg gcgatgttcc tgcttgccgg actgcatctc gatctcgtgg
    26161 tggcgcgggt gttccacgat tccgccggcg gcggctgggt cgcccgcgac gctttctggg
    26221 caaatggcat tgtacatgcg ggtggcggat atgcggtgcg cgcgatcggt ctcggtgcgc
    26281 tgctggcctg ggcgctcggc tggtggatcg ccccactcgc gcgataccgg cgcgcactcc
    26341 tctattttgt gctggcgctg gcgctgacca acggcctcgt cggcctgctg aaacagctca
    26401 ccaacgtaga ttgcccctgg gatctcgcac ccttcggtgg caggtttccc tatgtcccgc
    26461 tgctcgccga ccggccggac gagttgcgtc acgcggcatg cttccctggt gcgcattcgg
    26521 cgtccggctt cgcccttgtc gcgctctact tcgtgctgcg tgattgcgcg ccgcgccgcg
    26581 cgtggctcgg ccttgcgatc gggctctgca ccggcgccgt gtttgcgttc gcacaggagg
    26641 cgcgcggcgc gcacttcctg tcgcacgacc tgacgagcgc ggccaccgcg tggtacgtga
    26701 gcctcgtgct ctatgccctg tttcaggcac ccatcattca gagtcgcagc acgaccttgc
    26761 cggcattgcg cccggcgagc aggtattcga ccgcgtcggc ggctgactcg agcccggtga
    26821 acggggtcgg atcgaccagc gcgcgcagct cgccccggta gtagagatcg aggatgcgcc
    26881 tcgccgcctc atcgaagtac tgcgggaagg cgggattctg gaaggcgcgg atcgaggccg
    26941 atttccagta cagcttgtga taggcgcgcg gatgcggcac gtcctcgatg ggcttgtcga
    27001 agtccgaagt atggccgctg acgatcaacc gtccgtacat cgcgagatgg tcgaggaagg
    27061 cgtcgaagat ttcgccgccg accgagtcgt aggcgatgtc gaggccgcgc gggtattcgc
    27121 gtgcgagcac ttcgcgtagc gactccgtgc gatagttcac cacccggtct gcgccgagct
    27181 cgcgcagcag cgcggccttg tggtcggagc ccgtgagcgc aatgacgtgg ttgccggcgc
    27241 gcttcgcgag ctgcacgacg aaatgcccga ggccgcccgc ggctgccgac accgccacga
    27301 tctcgccgct tttcatgtcg gcgatgcgct cgagcgcaac catcgccgag acgccgctcg
    27361 ccatcagggc gaggatctcc ggcgtggcct cgcgaacccg gatcatcttg ccgacgtcgg
    27421 c
//
LOCUS       DAOQVY010000023        27098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_2087_length_27098_cov_6.409348, whole genome shotgun sequence.
ACCESSION   DAOQVY010000023 DAOQVY010000000
VERSION     DAOQVY010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 27098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27098
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2087_length_27098_cov_6.409348"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PKN91_03760"
     CDS             <1..779
                     /locus_tag="PKN91_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HNS27219.1"
                     /translation="IGRKINMMEQVLDVPRQEVITKDNAMVSIDAVVFFQVLDAPKAA
                     YEVDNLRLAIINLTMTNIRTVVGSMDLDETLSKRDEINHRLLKVVDDATTPWGIKVTR
                     IEIKDIQPPADLVESMARQMKAERDKRANILDAEGFRQAAILKAEGEKQSVVLAAEGS
                     KEAAFREAEARERLAQAEARATAMVSQAIAQGDVQAINYFVAQKYVEALKDFATSPNQ
                     KVMFLPLEASALLGSLGGIAEIAKEAMTKQGDGAAPRVRG"
     gene            782..1228
                     /locus_tag="PKN91_03765"
     CDS             782..1228
                     /locus_tag="PKN91_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNS27220.1"
                     /translation="MKEFLMQIEWWHWWIAAAVLAALETFMPGAVAIWFAVAAAVVGA
                     LVLVLPVPWQLQLVLFGALGLGAIFAYRAYARRNPATSAQPNLNQRGAQYVGRELTLV
                     EPIQQGFGKVRVGDTVWKVTGPPLGIGAKVRVTGVDGAVLIVEAAP"
     gene            1260..1685
                     /locus_tag="PKN91_03770"
     CDS             1260..1685
                     /locus_tag="PKN91_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS27221.1"
                     /translation="MLVRHVLDRKGSAVHAIGPDATVLEALRLMATHGIGALLVMQGD
                     KVLGVISERDYARKVALLGRASHDTPVSQIMTSPAVTVAPDDTVDSCMRVVTDKRIRH
                     LPVIANGRVVGVLSIGDLVKTLIGEQRETIEQLEAYIRS"
     gene            1720..3531
                     /locus_tag="PKN91_03775"
     CDS             1720..3531
                     /locus_tag="PKN91_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNS27222.1"
                     /translation="MRLPWLLSALLAGLGACLPAVCAPAAATAARCETAWSCEGVARI
                     VAVGDVHGALAEFTSILRAAGIIDDAGNWAGGESFLVSTGDLIDRGPESLAVIELLRR
                     LETEAQAAGGRVLVTLGNHEEMNLIGDLRYLVAADYAAFASKEAAAGEGAAGEGAAAA
                     SRLPPGWRARQQAFSPDGDIGQWLLARPLMVRVNGVLFTHGGLPPTLAGTSLEELNTS
                     SLHRDLVDYAVTWHALIAAGLLDADTDDAARVALAKERLAARAGADRERLAALVATLE
                     RTDASPVLGIAGPLWYRGSAMCHSAFETDTVSSVLAQLEAGSVVIGHTITTTRRITTR
                     MADRVTMIDTGMLPEYRGQPSALVIEGDARGVIYARAPGERAVPEREARRVGERPGGL
                     TDDQLEEFLHHADVVANEAPPPERAGARLLTLRRGDLRLRALFITAPPAGRWQHEVAA
                     YRLDRLLGIDMVPVTVERRIDGEAGALQFWIEKAASAAIVERDRIRLPGWCSLESQYG
                     LMRAFDALAGFAGRRSENVLLGREDGLARLVDFAAAFSSARGLDRRVAQPPVGAELAR
                     RLATLDAAKLAESLSPWLGRAQQRAILARRDALLAGR"
     gene            complement(3546..4055)
                     /locus_tag="PKN91_03780"
     CDS             complement(3546..4055)
                     /locus_tag="PKN91_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27223.1"
                     /translation="MPGTEENINKALFSTPEDRYGDDYRYHSLRQYQMYVEMADRVSN
                     RRMLANSFFVGMNTAVVGALVFALRAGLITTRALVLAPACAAILMCAAWWMMVLSYRQ
                     LNAGKFRVILAMEKGLPLAPYAAEWVALEADRSRRDYLQITRVELLVPWLFAGIHATL
                     TVAIIAKAF"
     gene            complement(4067..5770)
                     /locus_tag="PKN91_03785"
     CDS             complement(4067..5770)
                     /locus_tag="PKN91_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27224.1"
                     /translation="MELPAAIPLVIGVTGHRDLVDSELPAIRERVHEFLQGLRERWPH
                     TPIVVASQLAEGADLLVAEEALALGLELVFLQPLPLEDYRSQMTDDAARGRFDKLRAA
                     SRVLDLASGEASYDRDTLYASAGDFLARYCYILLALWDGKPAASPGGTAAVVDYRLAS
                     RGGVRPAVEDLRDLALGEVDNSLVFHILVSRARPNGKPTNGHHPLAAGYVHEFAGGRS
                     ALHDAMPDLNRHVLDRTEEFNAAAAAAGDLAGPGSVPFVGAPPAIARCARLIAVADGL
                     ATQHRRRLMRITAWTYAIGAVMGCAFVLYSKIPAMWGLIYLFFGAALTTIALSRFAEG
                     RGWHRDHLEYRALCEGLRVQFYLRIAGVRDEGRLHTGDESFLRRQDTELDWIRTAMRA
                     VDFEMHDAGRTGVSGGIEHVIEHWVGSLDLTKDRGGGQLAYFWRAGERRRRMIALTER
                     IGAVTLVLGLTAAAVLCFIPVLHVPYVKTPLVVTMGLLPLVAGLLEAWLQKTAAKELA
                     RQYGYMYRLFDEARTHLQGAAGDERRRAVLYSLARAALSEHATWVLLNRDRKLRHLPS
                     H"
     gene            complement(5793..9125)
                     /locus_tag="PKN91_03790"
     CDS             complement(5793..9125)
                     /locus_tag="PKN91_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS27225.1"
                     /translation="MDTFRIFLSSPGDVAEERLIARRVIDRLGSEFNGRVRIEPVFWE
                     HEPLVASASFQDQLPHPADAHAAICVLWSRLGTRLPASFRKPDGKHYESGTEFEFEDA
                     LSGYRRHGRPALLVYRKTAPPLLDADDDAQSLERLRQKQALDAFVRRWFHDETDGTLR
                     AAFHPFADVAQFEELIDVHLRKLIARTFPEAVGAARTDVATWRHESPFRGLEAFDFEH
                     APVFFGRTAAIGEMIAALRRQDAADRPFLLVIGASGSGKSSVVRSGLLPVLTQPGVIE
                     GIAEWRRVELRPRDRRDDLCALLEESLGEAAAAAPPDRRTVLVIDQFEEVFADERIGH
                     AERERFFARLAQVTASRKVWTIATLRSDFYPRCVEHGTLVALKAGDGQYDLRAPTPAE
                     IGQIIRLPAAAAGLRFETRVDGGEGLDEVLRDEAARSPEALPLLEFALEELYLRRASD
                     GTLTFAAYHDIGGVEGALARRAEAVFMALPEATRESLSSVLRRLISATTTGAGAFSRR
                     SAALDEFPAGPQWDLVHALIEARLCVSELGNDARPVVSIAHEALLRHWPRVRDWLGEN
                     RDLLRARARLAANAERWEQESRRADLLLAAGRSLEEAASVAAAGMELTPSEVALIEAS
                     QARARRARLARNSAVAGLAVLATAATTAALVATSQRERAETEASTTEQTMDFMVSLFT
                     LADPTEARANEFTVREMLDRGAADVQRQLQDRPAIRSSLMTAMGRAYTGLGLPAPAQA
                     LLRDALVLRERSAGADTRATTETRRALAAATYLDGAYVEAEKQYRIALASARQLAPEG
                     DALVSRILVGLGDTLSQLGSDADAETAYREALAIDTRLHGAKSLEVAETMQGLAITLY
                     YEARYGEAEQLTRDSLAIRRNSLGRGHWKVADSLNHLGAISFQTGQYEEAVTAWQECL
                     AISDEWAGKDHPESASVANNIGRGLLIEGRLDEAEGYLARALDTHRRTKPPDHDDLIL
                     PLNSLAMIDLARRRYAGAEARLDEALGIAEKRNHWMRDQVLTNLADVYAKTDRAPQAG
                     ELLSRARTLLEQQYPLAEMPDEAWRYALQDAVLATTYAAEKKYPEARRLLTEALPIVR
                     KRFGGERYYTRDLEQRLARLPR"
     gene            complement(9196..10113)
                     /locus_tag="PKN91_03795"
     CDS             complement(9196..10113)
                     /locus_tag="PKN91_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNS27226.1"
                     /translation="MSTNRVSRALIAAVAVALLPIGTAEARRVQVDFGPESFPQSGAG
                     WPGRPGVGFDSSFDDGVDLTSAPADGSLLFTTEYRDSDDTLLGGPYSSHALNIGGTSY
                     DSLRMFEDGSFALFDSATGYSAADPLFGVFAADLASNAYDLGDGTFSSNGDGSTSYTF
                     GFGMGLSDAAPDADAAVAGIRFFWTNIALADDPDTTYDFQAYIYFLGNGDFDVDMRYG
                     SNALDSSFPSLIGNQFITAGGADLFRSTDALVEDADYFFRFRDGVLVGGTTPPPPAPV
                     PEPGTLVLMLAGLAALLMIRRRTGQPAAR"
     gene            10331..11299
                     /locus_tag="PKN91_03800"
     CDS             10331..11299
                     /locus_tag="PKN91_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27227.1"
                     /translation="MECGVRSWSVGAAVLYGALGLLPLPAAARESCALDALASPDLVI
                     ANVEPVRFANDITRKAIAHTATDPAAFKIKVRRAGPGASSFNNEPRYELAALEISRLL
                     YPPGEDVVPPIALRSESFPAFYAIDPAGVEAWRGTNAALFMVQCWLEGAKSHRLEIDR
                     SRFATDPAYARAISRLNVLTYLIEHKDSNLGNVMLTGEGETVRAWAIDNGVAFDSAES
                     DVGVFWRNLRVDAVDRALADRLRSLTLEELRARLGVIAQYELVDSRWQPRERGANLDA
                     RRGVRRRANQLQLGLTSAEINSIERRRQRLVRRIDKGEISLLPDAS"
     gene            complement(11274..12908)
                     /locus_tag="PKN91_03805"
     CDS             complement(11274..12908)
                     /locus_tag="PKN91_03805"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNS27228.1"
                     /translation="MSAPAELTPFERALRLLTRVRAGEGRCIALFGSHAFLIMASYYV
                     LKALRESFLLAGAAAEVRSYAVAANGVVLMLLVPLYSAVRRRIDGRRLVGLVSAFFAA
                     NILAFAVAYPFRASKWFGIVFFIWVSVYGLMVVAQFWALAASSFNTKSGQRLFPAIML
                     GATVGALAGAQITDLAIVRLGPLPLLLLGALLLVASIGVISPEARAVPESSRPVPVTG
                     AAKTVSSVFGGFQVVFSSRYLLLVATFVVLLNCIGSTGDYLLAALIKARAEMLAGADA
                     LLTEREAIIGSLYARFQLWVTLVGVLVQALAVSRVYRAIGVRGAVLVLPAIALFVYGA
                     IAFVPVFTMIYVAKILENSTNYSLMNTTEQTLYLPTGAAAKFDGKTTIDTFFWRFGDI
                     LQAGVVFAGLNWFGFGTRSFAMLNAALCVVWLGVAARIGRHYSALSRAETQNTAPVRQ
                     REPEDLLVHPGRAIDHVLPAETFFDPDPGDVLAYEARLAGGGPLPPWLEFDGALARLT
                     GVPPGDVLVEEYIEIVASDMDGATATAIIRMRRVTG"
     gene            complement(12977..14425)
                     /locus_tag="PKN91_03810"
     CDS             complement(12977..14425)
                     /locus_tag="PKN91_03810"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNS27229.1"
                     /translation="MFGVLYVLGLMLALFASTFLLPIACSLLTGDGMLVHFVVGATVI
                     VLVGLGLALAFRRHKRELKARDGFLLVTLAWVMMSAAATVPLLMALPDLSVTDAFFET
                     MSGLTTTGATVLTHIESLPPSINLWRHALHWYGGLGIIVLAVAVLPLLGVGGMQVYKA
                     ESPGPVKDEKLTPRITQTAKALWLVYATVTLVGVLALRLAGMNWLDAICHAFSAMALG
                     GFSTYDSGIGHFDSAAVEIVLIALMLFACVNFSRHFMVVQRRSLKPYARDAEAKAIML
                     VLGVSVAAITALLWASQTYAGFGASLRHAAFTVVSIATTTGFVTDNYEAWPVFAPVWL
                     LFLSCIVCSTGSTGGGIKMFRTLLLQRHAFREMKMLLHPRAIVPIRIGGIVIPDRVVF
                     SVLAFISLYFATVVALTFALLLSGLDLVSAFTGVVASINNLGPGLGQAGPTTQYQSFT
                     DFQTWMFSLAMLLGRLEIFSVLVLFTPAFWRN"
     gene            complement(14432..15805)
                     /gene="trkA"
                     /locus_tag="PKN91_03815"
     CDS             complement(14432..15805)
                     /gene="trkA"
                     /locus_tag="PKN91_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNS27230.1"
                     /translation="MKIVILGAGQVGRTAAYHLSREEANDVTVVDTNEQVLRDLQGRL
                     DIRTVAGSGSFPSVLEAAGAARAEVLIALTNSDEINMLACEIAYTLYRTPTKIARIRS
                     SEYASTPALFSAEALRVDVWISPEQLVTEYIERLIRYPGALQVLTFAAGRVRLVGVRA
                     ETGAPLVGQPIRMLREHLPDFDSHIVAVYRDGRRIEPGPETVIEADDEVFLFATREHM
                     RRVMDELRVAGEAARRIVIAGGGSIGLRLAHALEDSHHVKVIERNADRARYVAERLES
                     AIVLNGDAADEELLIEENIDNSHVFAAVTNSEEANILSAMLAKRLGARKVMALINKPS
                     YAELMESGSIDVAISPQTVTIGSLLAHVRRGDVVRVHSLRHGSAEVMEAIAHGRAGSS
                     RIVGVAVGDIPLPDSATIGAITRGNDVIMAKDDTVVQENDHVIIFLGDRRHVDQVEKL
                     FVSGTSP"
     gene            complement(15810..18026)
                     /gene="uvrD"
                     /locus_tag="PKN91_03820"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(15810..18026)
                     /gene="uvrD"
                     /locus_tag="PKN91_03820"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HNS27231.1"
                     /translation="MDLSPILSPLNDAQRAAVTAPVGPVLVLAGAGSGKTRVLTHRIA
                     WLVQAEGASPHSILAVTFTNKAAGEMRARIEALLGIPGSSLWVGTFHGIAHRLLRLHW
                     REAGLPQGFQILDAEDQLRMIRKLLRAQGLDESRWVPREVTWFINHQKDEGLRPKALK
                     DGNDPTRGQLIKLYRDYEEACARAGVVDFAELLLRAFELLRDNVEVLRHYRTRFRHVL
                     VDEFQDTNAIQYAWLKLLVGSEGAPFIVGDDDQSIYRWRGARVENLQQFQRDWPQAIL
                     HRLEQNYRSTGNILKAANELIAHNSGRLGKNLWTSGGHGEPIRLYAAFNERDEADFVT
                     HRIRDWVQRGGARRDIAILYRSNAQSRVFEEAFLSARIPYKVYGGLRFFERAEIKDAL
                     AYLRLLSNRDDDASFERVVNLPARGIGAKTVDELRVAARAAGISLWQAARQAIVDGTL
                     AARATGALGGFVQLIERLSADAANLPLHEQVDLVLAASGLVEHHRKEKADRGEARVEN
                     LEELVSAARGFEPEGDLPPLDAFLAHAALESGEGQAEEWEDCVQMMTLHTAKGLEFPV
                     VFLCGMEDGLFPHQRSLNDLDGLEEERRLCYVGMTRAMQQLYVTYAEQRRLHGMDSYG
                     QASRFLAEIPEELVEEVRPRIQLGRPYARSGHPAPTHGDRGGAAWSPGQAGRRAIAAE
                     PVAPGMRLGARVRHGKFGEGTVLNVEGQGPQARVQVNFERQGTKWLMLAYANLEAM"
     gene            complement(18038..19096)
                     /locus_tag="PKN91_03825"
     CDS             complement(18038..19096)
                     /locus_tag="PKN91_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNS27232.1"
                     /translation="MDSPFYRRGFAVAALLLVGYLLFRVLEPFLGPMVWAAFLAFMLY
                     PLHERWTRCFKGRATMSAALITVLTPVAILIPLAVIGGVFAAQAAELAGRARGFVQGL
                     GVGGLTQFEQYPVVGPVLLWVDEHTGFGAQELRDWLLSSGESFLKWGATIGGNVVVGA
                     LGTVLAFAIAIVLLYFFLCDGAAWIRRGVRLIPMQPERRDGLVDHLAQVTRAVVFGSG
                     ITALLQGAAVGVGFAIAGLSSFVVFGVLAALFALLPFGGAAIVWIPGVLYLAMQGRWG
                     MAIFLLIWGLGVSSADNLVRPLLVSSRARISTLTVFIGVLGGAAAFGAIGLIVGPLVL
                     TLAAALLDYMDERSEPPA"
     gene            19131..19751
                     /locus_tag="PKN91_03830"
     CDS             19131..19751
                     /locus_tag="PKN91_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNS27233.1"
                     /translation="MKLYDYLDSGNGYKVRLVLAQLGQSCDWVECDILQGATRTPEFL
                     ARNPNGRIPLLELDDGSFLAESNAIIWYLAEGSPLVPDDRLLRAQVLQWMFFEQYSHE
                     PYVATPRFIVRHLPADSPRRAELPERLERGRAALAVMDRHLGGQRFFVGHRYTIADVA
                     LYAYTHVAHEGGHDLAPFAHVRAWLDRVQSQPGHILLAARPAPRRA"
     gene            complement(19758..20549)
                     /locus_tag="PKN91_03835"
     CDS             complement(19758..20549)
                     /locus_tag="PKN91_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HNS27234.1"
                     /translation="MLIERIWTANDYRNFHYLVACAETGEALAIDPLDWRKCVAAARA
                     RGYEITQILNTHEHRDHTGGNEPLREATGARVLAHAGAAARIGHVDVGLTRGDVVRVG
                     RTVELECLDTPGHTLAHLCLYGRGETPVLFCGDTLFNAGAGNCHHGGHPELLYETFAQ
                     QLARLPDATRIYPGHEYLERNLAFTLDREPGNEIAATMHRDAQVQDPAAAPITTLGEE
                     KRINTFFRLQNPQIVARLREACPDLPDQPSPRDVFLKLRELRNRW"
     gene            complement(20554..22620)
                     /locus_tag="PKN91_03840"
     CDS             complement(20554..22620)
                     /locus_tag="PKN91_03840"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HNS27235.1"
                     /translation="MHDEPRQFLAKWPPLAVAAGFFPVHLAAAWLGSHLMIGAHSAAV
                     IWPGAGTYIAALMLFPRRYWPWLFASGLLAEVCAFALVHSVFNPLAGTLVGLVNIGQA
                     VFAAWLIGRHIGPRVDFSRTGPTARLTFILLVATFVGGVIGAAVATAAGTAAYWSNQQ
                     TWWFSGYVGAVIIVPLAVSWAESGVRAAPAQPPPRAWYVLLQFTVLALLLWFVFGMEH
                     RFETFALDSPYVVYAALLWISANCGARRITLALFLVALVSATCTLAGRGPFAVTPGEP
                     FGATLNLQAFLALVVVPLLVVQASIAEKRDALQLVRASDERYRAFVSQSSEAIFRLEL
                     SQPMPLLLSAKEQRQWLDKYAWIAEANDAYRKAEGTGEVTDRITRHPPWLKHCLATVP
                     DAIIAGGRVENLECVLPGPYGLDRTLLVSLTTEIDHGALRRLWGVARDITHYRAAQRR
                     LEQQQRKLRALATELTLTEERARRKIAADLHDGLAQSLIGLQMHVAAIRATVEQGRPL
                     PDLSALELTISESTAQVRALLSDLMPPGLSDQDIVSGLRWLAEEFAKRQRVQVEFEDD
                     GAQKPLAESTAVLIYQAARQLLQNVARHAQATVVAIRTRVADGQFELGISDSGVGFNV
                     SDLTFLPTEQGGFGLYSIRERLAIIGGTLEIDSSPGRGTRVRITAPLEPDDHDHRVIG
                     RTGMGG"
     gene            complement(22675..23565)
                     /gene="cyoE"
                     /locus_tag="PKN91_03845"
     CDS             complement(22675..23565)
                     /gene="cyoE"
                     /locus_tag="PKN91_03845"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNS27236.1"
                     /translation="MSWRDYFELTKPRVVYLIVFTAIVGTLLASPGWPPLGPLVWGNL
                     GIALAAACAAAINHVLDQKIDEKMARTRNRPIPKGKISTARALLFAAALGVLSMLILW
                     ILVNPLTSVLTFFSLIGYAVIYTAWLKRATSQNIVIGGAAGAAPPVLGWAAVTNSIDP
                     DALLLFLIIFVWTPPHFWALAIARKDEYARAGIPMLPVTHGVAYTRLQVLLYTVLLVL
                     VTLMPFVTGMSGLIYLVAALVLNGIFLYYALAMKFTDRQELPMRVFKFSVTWLMWIFI
                     ALLVDHYLPTTQLIADSLRS"
     gene            complement(23562..24554)
                     /locus_tag="PKN91_03850"
     CDS             complement(23562..24554)
                     /locus_tag="PKN91_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNS27237.1"
                     /translation="MTVTTLARRLALAAMLLCLIVVVLGAYVRLSDAGLGCPDWPGCY
                     GHITPIGALANDAAVQERFPGAEVHAGKAWREMIHRYAATTLGLIIVVLTAIAIATRA
                     RRLVPLRLSVALLAVVVFQGILGMLTVTWLLKPLIVTAHLVFGLTTLAMLSWLWLSLR
                     PRPQNHDAPRLVRRWAIIAIVALALQIALGGWTSSNYAAMACPDFPTCQGRWWPGMDF
                     GDAFVLWHGLGINYEGGVLDHPTRVAIHFVHRLGAIVASIALLIAAATALARIRTPGT
                     VRAAVFVIAALALQLLIGIFMVWRGFPLWLATAHNAGAALLLLSAVLLNRRVRA"
     gene            complement(24568..25116)
                     /locus_tag="PKN91_03855"
     CDS             complement(24568..25116)
                     /locus_tag="PKN91_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27238.1"
                     /translation="MNNANARGLRTLIGLAALFFVPLFIAFALYYGGGWRPAQSTNHG
                     VLLADPVALEVPWPESKWTLLLVASDACSDSCREALYKTRQTHLLLAKDFDRVQRVFI
                     APADCCETDFLASEHPGIITLDPAGSAGSALLGALPAGTPDNTIYLIDPLGNLIMTFD
                     SHDDPKGLLTDLKKLLGLSHVG"
     gene            complement(25113..25799)
                     /locus_tag="PKN91_03860"
     CDS             complement(25113..25799)
                     /locus_tag="PKN91_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HNS27239.1"
                     /translation="MTALAVLSIAVFVTCGAWQWNRGVARQAVWQDFATDAAPTIDPG
                     MQALEALPRFTRVRLEGRYDGEHQILLDNRTHAGRAGYEVLTPFELHDGRLVLVNRGW
                     VPFTGYRDRLPDVALAGTTGSVTVTGRVDTLPVQGMAAGHAAPDPDASWPKLTSYPRH
                     DELVAVLGRAVETRQVLLDADAPDGYIRQWQAPGIPPERHFSYAAQWWLFAATVAVLY
                     VILNLHKTDR"
     gene            25952..26158
                     /locus_tag="PKN91_03865"
     CDS             25952..26158
                     /locus_tag="PKN91_03865"
                     /inference="COORDINATES: protein motif:HMM:NF022587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HNS27240.1"
                     /translation="MELIQIFVVVVLIAIVASLGSALFHLSSPGRDPKKMVRALTWRI
                     ALSVSLFLLLMLAWQLGYITPHGL"
     gene            complement(26171..27082)
                     /gene="arcC"
                     /locus_tag="PKN91_03870"
     CDS             complement(26171..27082)
                     /gene="arcC"
                     /locus_tag="PKN91_03870"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010635806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNS27241.1"
                     /translation="MSRKTIVVALGGNALLKRGEPLDVPTQRRNAQRAAASIATVAAG
                     NELVVTHGNGPQVGLLALQSAAAGDASYPLDVLGAQTEGMIGYVLEQELMNELGERRA
                     ATLLTQTEVDPADRAFERPTKFIGPVYAATEAQRLAAERGWRIAPDGGHWRRVVPSPE
                     PVDILELGAIELLLGAGYVVICAGGGGIPVGRSAAGSYEGIECVIDKDLTSELLAERL
                     HADCLLMLTDVPAVFIDWGTPQARELRAATPGELAQYTFPAGSMGPKVEAARRFATSG
                     PGRRACVGSLDDVAGILAGRAGTTIRS"
BASE COUNT         4497 a         9101 c         9424 g         4076 t
ORIGIN      
        1 ccatcggccg caagatcaac atgatggagc aggtgctcga cgtgccgcgc caggaggtca
       61 tcaccaagga caacgcgatg gtcagcatcg atgccgtggt gttcttccag gtgctcgatg
      121 cgcccaaggc cgcctacgag gtcgacaacc tgcgcctcgc gatcatcaac ctcacgatga
      181 ccaatatccg cacggtcgtc ggttcgatgg acctcgacga gacgctgtcg aagcgggacg
      241 agatcaacca ccgcttgctg aaggtggtcg acgacgcgac gaccccgtgg ggcatcaagg
      301 tgacccgcat cgagatcaag gacatacagc cgccggccga cctcgtcgaa tcgatggccc
      361 gccagatgaa ggccgagcgc gacaagcgcg ccaacatcct cgacgccgag ggcttccgcc
      421 aggccgcgat cctgaaggcc gaaggcgaga agcagtcggt ggtgctcgcc gccgagggca
      481 gcaaggaagc ggcgttccgc gaggccgagg cccgcgagcg gctcgcgcag gccgaggcgc
      541 gcgcgaccgc gatggtctcg caggcgatcg cgcagggcga cgtgcaggcg atcaactact
      601 tcgtcgcgca gaaatatgtc gaggcgctca aggatttcgc gacctcgccg aaccagaagg
      661 tgatgttcct gccgctcgag gcgagcgcac tgctcggcag cctcggcggc atcgccgaga
      721 tcgccaagga agcgatgacg aagcagggcg acggcgctgc gccgcgcgtg cgggggtagc
      781 gatgaaggaa ttcctgatgc agatcgagtg gtggcactgg tggattgccg ccgcggtgct
      841 cgccgccctc gagactttca tgcccggggc ggtcgcgatc tggttcgcgg tcgcggccgc
      901 ggtcgtcggg gcgctggtgc tggtcctgcc cgtgccgtgg cagctgcagc tcgtgctgtt
      961 cggtgcgctc gggctggggg cgattttcgc ctatcgcgcc tacgcgcgac gcaatccggc
     1021 aacgtcggcc cagccgaacc tgaatcagcg cggcgcgcag tacgtcggcc gggagctcac
     1081 gctggtcgag ccgatccagc agggcttcgg caaggtgcgc gtcggcgata cagtctggaa
     1141 agtcaccggg ccgccgctcg gtatcggcgc gaaggtccgg gtgacgggag tcgatggtgc
     1201 ggtattgatc gtcgaggccg caccgtaagc ccaacgctgt agcggcagag gaggtcgcca
     1261 tgctggtacg tcacgtgctc gacaggaagg gctcggcggt gcatgccatc ggaccggacg
     1321 ccacggtgct cgaagcgctc aggctcatgg ccacgcacgg catcggcgca ttgctggtca
     1381 tgcagggcga caaggtgctc ggcgtcattt ccgagcgcga ctacgcgcgc aaggtggcgc
     1441 tgctggggcg ggcgtcgcac gacacgccgg tctcgcagat catgacctcc cccgccgtca
     1501 ccgtggcgcc cgacgacacg gtcgattcgt gcatgcgcgt ggtcaccgac aagcgcatcc
     1561 gtcatctgcc cgtgatcgcc aatggccgcg tggtcggcgt gctgtcgatc ggcgatctcg
     1621 tcaagacgct catcggcgag cagcgcgaga cgatcgagca gctcgaagcg tacatacgga
     1681 gctgagagcc cggggcgggt gcccggagcc agaggaccaa tgcgcttgcc gtggctgctg
     1741 tcggcgttgc ttgcggggct cggggcctgc ctgccggccg tgtgcgcccc ggccgcggca
     1801 accgcggcgc gctgcgagac cgcgtggtcg tgcgaaggcg tcgcgcgcat cgtcgccgtg
     1861 ggggatgtgc acggtgcgct ggccgaattc acctcgatcc tgcgtgccgc gggcatcatc
     1921 gacgacgcgg gtaactgggc gggtggcgag agcttcctcg tcagcaccgg cgacctgatc
     1981 gaccgcggac ccgaatcgct tgcggtaatc gagctcctgc ggcgcctcga aaccgaggcg
     2041 caggccgcgg gtgggcgcgt gctcgtgacc ctcggcaatc acgaggagat gaacctcatc
     2101 ggcgatctgc gttacctcgt cgcggccgac tatgccgcgt tcgcctcgaa ggaggccgcg
     2161 gcgggcgaag gcgcggcggg cgaaggcgcg gcagcggcga gccggctgcc gcccggctgg
     2221 cgcgcgcgcc agcaggcctt cagcccggac ggcgacatcg gccagtggct gctggctcgc
     2281 ccgctgatgg tgcgcgtgaa cggcgtgctg ttcacccacg gcggcctgcc gcccaccctc
     2341 gccggcacat ccctcgagga actcaacact tcgtcactgc accgggatct ggtcgactac
     2401 gccgtcacat ggcatgcgct gatcgccgcc ggcctcctcg atgccgacac cgatgacgcg
     2461 gcgcgcgtcg cgctcgcgaa ggagcgcctg gccgctcgcg cgggcgccga tcgggaacgc
     2521 ctcgccgcgc tggtcgcgac gctcgagcga accgatgctt cgcccgtgct cggcatcgcc
     2581 gggccgctct ggtatcgggg cagcgcgatg tgccacagcg cgttcgagac cgatacggtg
     2641 tcgagcgtgc tcgcccagct cgaagcgggg agcgtcgtga tcggccacac gatcaccacc
     2701 accaggcgca tcaccacccg gatggcggat cgcgtcacga tgatcgatac cggcatgctg
     2761 cccgagtacc gcggtcagcc ctccgcgctc gtgatcgagg gcgacgcgcg cggcgtgatc
     2821 tacgccaggg cccccggcga gcgtgccgtg cccgaacgcg aggcgcggcg ggtgggcgag
     2881 cgcccgggtg gcctcaccga cgatcagctc gaggagttcc tgcaccatgc cgatgtcgtc
     2941 gcgaacgaag caccgccgcc ggagcgcgcg ggcgcgcgcc tgctgacatt gcggcggggc
     3001 gacctgcgcc tgcgggcgct cttcatcact gcgccgcccg cgggccgctg gcagcatgaa
     3061 gtggcggcct atcgactcga tcgcctgctc ggcatagaca tggtgccggt caccgtcgaa
     3121 cgcaggatcg acggcgaggc aggtgcgctg cagttctgga tcgagaaggc ggcaagcgcc
     3181 gcgatcgtcg aacgtgaccg gatacggctg ccgggctggt gcagcctcga gtcgcagtac
     3241 gggttgatgc gcgcattcga tgcgctcgca ggattcgccg ggcgccgatc ggagaacgtg
     3301 ctgctcgggc gcgaagacgg cctcgcgcgc ctggtcgatt tcgccgccgc cttcagcagc
     3361 gcgcgcgggc tcgaccggcg cgtcgcgcag ccgccggtcg gcgccgaact cgcgcgccgc
     3421 ctcgccacgc tcgacgcggc gaagctggcc gagtccctgt cgccgtggct cggtcgcgcg
     3481 cagcagaggg cgatcctcgc gcgacgcgat gcgctgctcg ccgggcgctg agcgccgccg
     3541 gcggtctaga acgccttcgc gattatcgcc acggtgagcg tggcgtggat gccggcgaac
     3601 agccacggca ccagcagctc gacccgggtg atctgcaggt aatcgcggcg cgagcggtcg
     3661 gcttcgagcg cgacccactc ggctgcatag ggtgcgagcg gcaatccctt ctccatcgcg
     3721 aggatcaccc tgaacttgcc ggcgttcagc tgccggtagg acagcaccat catccaccac
     3781 gccgcgcaca tcaggatcgc ggcgcatgcg ggcgcgagca ccagtgcgcg cgtcgtgatc
     3841 aggcccgccc gcagcgcgaa caccagcgcg ccgaccaccg cggtgttcat gccgacgaag
     3901 aaagagttgg cgagcatgcg ccggttgctg acacggtcgg ccatctccac gtacatctgg
     3961 tactggcgca gcgagtggta gcggtagtcg tcgccgtacc tgtcctcggg cgtcgagaac
     4021 agggccttgt tgatgttctc ttcggtaccg ggcatggccg tggttcctag tgtgaaggca
     4081 ggtggcgcag tttgcgatcg cggttcagca gcacccaggt cgcgtgttcg gacagggccg
     4141 cccgcgcgag tgaataaagc acggcgcgcc gccgctcgtc cccagctgcg ccctgcaggt
     4201 gcgtgcgcgc ctcgtcgaac aggcggtaca tgtagccata ctggcgggcc agttccttgg
     4261 cggcggtctt ctgcagccag gcttcgagca gaccggccac gagcggcagc aggcccatcg
     4321 tgacgacgag cggcgttttc acgtagggaa cgtgtagcac cgggatgaag cacagcaccg
     4381 cggccgcggt gaggcccagc acgagcgtga ccgcgccaat gcgttcggtg agcgcgatca
     4441 tgcgccggcg ccgttcgccg gcgcgccaga aataggcgag ctggccgcca ccgcggtcct
     4501 tggtcaggtc gagcgaaccg acccaatgct cgatcacatg ctcgatgccg ccgctcacgc
     4561 cggtgcgccc cgcatcgtgc atctcgaagt cgaccgcgcg catcgcggtg cggatccagt
     4621 cgagttcggt gtcctggcgg cgcaggaaac tctcgtcgcc ggtgtgcagc ctgccctcgt
     4681 cgcgcacgcc ggcgatacgc aggtagaact gcacgcgcag cccctcgcac agcgcacgat
     4741 actcgagatg gtcgcggtgc cagccacgac cctcggcaaa acggctgagc gcgatggtcg
     4801 tgagcgcggc tccgaagaac agatagatga gtccccacat cgccgggatc ttcgaataca
     4861 gcacgaacgc gcaacccatc accgcgccga tcgcataggt ccacgcggtg atgcgcatca
     4921 ggcgccgccg atgctgggtc gcaagtccgt ccgcgaccgc gatcaggcgc gcacagcgcg
     4981 cgatcgcggg cggcgcgccg acgaacggta cgctgccagg tccggccaga tcgccggcgg
     5041 cggcggcggc ggcgttgaac tcctcggtgc gatcgagcac gtgccggttg aggtctggca
     5101 tcgcatcgtg cagggcggag cgtccgccgg cgaactcgtg gacgtagcct gccgcgagcg
     5161 gatggtggcc gttcgtcggc ttgccgttag gtcgcgcgcg cgacacgagg atgtggaaca
     5221 cgagcgaatt gtctacctcg ccgagcgcga gatcgcgcag gtcctcgacc gcgggccgca
     5281 cgccgccgcg cgacgcgagc cggtaatcca ccaccgccgc cgtgccgccg gggctcgccg
     5341 ccggcttgcc gtcccacagc gcaagcagga tgtagcagta gcgcgcgagg aagtcgccgg
     5401 cactggcgta cagcgtgtcg cggtcgtagc tggcttcccc ggatgcgaga tcgagtacgc
     5461 gcgaggcggc gcgcaacttg tcgaagcgcc cgcgcgccgc atcgtcggtc atctgcgagc
     5521 ggtagtcctc gagcggcagc ggctgcagga acacgagctc gagcccgagc gcgagggcct
     5581 cctcggcgac cagcaggtcg gcgccctcgg caagctgcga ggcgaccacg atcggcgtgt
     5641 gcggccagcg ctcgcgcagg ccctgcagga actcgtgcac gcgttcgcgg atcgccggca
     5701 gctcgctgtc gacgagatcg cggtggccgg tgacaccgat gacgagggga atggctgcgg
     5761 gcagttccat ttgcggggcg gttctccctg tgtcagcggg gcaggcgggc aagccgttgc
     5821 tcgagatcac gcgtgtagta tcgctcgccg ccaaagcgtt tgcgcacgat cggcagtgct
     5881 tcggtcagca gcctgcgggc ttcgggatat ttcttctccg cggcgtaggt ggtggcgagc
     5941 acggcgtcct gcagcgcgta gcgccaggcc tcgtcgggca tctcggcaag cgggtactgt
     6001 tgttcgagca gcgttcttgc ccgggagagc agttccccgg cctgcggggc gcggtccgtc
     6061 tttgcgtaca catcggcgag gttggtcagc acctggtccc gcatccagtg gtttcgcttc
     6121 tcggcgatac ccagtgcctc gtcgagcctc gcctccgccc ctgcgtacct gcgccgcgcg
     6181 aggtcgatca tcgcgaggct gttgagtggc aggatcaggt cgtcgtggtc ggggggcttg
     6241 gtgcgccggt gagtgtcgag cgccctggcc agatagcctt cggcctcatc gagcctgcct
     6301 tcgatcagca ggccacggcc gatgttgttg gccacactcg cgctctcagg gtggtctttc
     6361 cccgcccatt cgtcggagat ggccaggcat tcctgccacg ccgtcaccgc ctcctcatac
     6421 tgcccggtct ggaaactgat cgcgccgaga tgattcagac tgtcggcgac cttccagtgt
     6481 ccgcgaccga gcgagttcct cctgatcgcg aggctgtcgc gagtgagctg ctccgcctcg
     6541 ccgtaccgcg cctcgtaata gagcgtgatc gcgagtccct gcatcgtctc ggcgacctcc
     6601 aggctcttcg caccgtgcag ccgcgtgtcg atcgcgagcg cctcgcgata ggcggtctcg
     6661 gcatcggcat ccgatcccag ctgcgacagc gtatcgccga ggccaacgag tatgcgcgat
     6721 accagtgcgt cgccctcggg cgcaagctgc cgtgcactgg ccagggcaat ccggtattgc
     6781 ttctcggcct cgacgtaagc gccgtcgagg tacgttgcgg cggccagcgc ccgccgcgtt
     6841 tcggtcgtgg cccgtgtgtc ggcgcccgcg gaccgttcgc gcaataccag cgcatcgcgc
     6901 aacagtgcct gcgccggggc cggcaggccg aggcccgtgt acgcccggcc catcgcggtc
     6961 atcaggctcg aacggatggc gggtcgatcc tgcaactggc gttgcacgtc ggcggcaccg
     7021 cggtcgagca tctcgcgcac cgtgaactcg ttcgcgcggg cctcggtcgg gtcagcgagc
     7081 gtgaacaggg agaccatgaa atccatggtc tgctcggtcg tcgacgcctc ggtctctgcc
     7141 cgctcccgtt gcgaggtggc gacgagagcg gcggtggtcg ctgccgtggc gagcacggcc
     7201 aggccggcaa ccgcgctgtt gcgggcgagc cgtgcgcggc gtgcccgcgc ctgcgaagcc
     7261 tcgatcagcg cgacttcgga gggcgtgagt tccatgccgg cagcggcgac actcgcagcc
     7321 tcctcgagcg atcggcccgc cgccagcaac agatcggccc tgcggctttc ctgctcccag
     7381 cgctccgcgt tcgccgccag gcgtgcccgg gcccgcaaca ggtcgcgatt ctcgccgagc
     7441 cagtcgcgca cgcgcggcca atggcgaagc agggcctcgt gcgcgatcga caccacgggt
     7501 cgtgcgtcgt tgccgagttc ggacacgcac aggcgcgcct cgatcaatgc atgcacgagg
     7561 tcccactgcg gaccggcggg gaactcatcg agcgcggcgc tgcggcggct gaatgcgccc
     7621 gcaccggtgg tggttgcgct gatgagcctg cgcaggaccg atgacaggct ttcgcgggtt
     7681 gcctcgggca gcgccatgaa caccgcctcg gcgcgccgcg cgagcgcgcc ctcgacgccc
     7741 ccgatgtcgt ggtaggcggc aaatgtcagc gtgccgtcgg acgcgcgtcg caggtagagc
     7801 tcttcgagcg cgaactccag cagcggcagg gcctccgggc tgcgtgccgc ttcatcgcgc
     7861 agcacttcgt cgagaccctc gccgccatcg acgcgcgtct cgaagcgcag gccggccgcc
     7921 gcggcaggca gccggatgat ctggccgatc tccgccggcg tgggtgcacg caagtcatat
     7981 tgtccgtcgc ccgccttcag cgcgacgagg gtcccgtgct cgacgcagcg cggatagaaa
     8041 tcgctgcgca atgtcgcgat cgtccagacc ttgcgcgacg ccgtcacctg cgcgagccgc
     8101 gcaaaaaaac gctcgcgctc cgcgtggccg attcgctcgt cggcgaacac ttcctcgaac
     8161 tggtcgatca cgagcacggt gcgtcggtcc ggtggcgcgg ccgcggccgc ttcgcccagg
     8221 ctctcttcga gcagtgcgca aaggtcgtcc ctgcggtccc gcggtcgcaa ctcgacacgc
     8281 cgccattcgg caataccctc gatgacaccc ggttgcgtga gtaccggcag caggccggag
     8341 cgcacgactg acgacttgcc gctgccgctt gccccgatca cgagcaggaa tggccggtcc
     8401 gcggcatcct gccggcgcag cgccgcgatc atctcgccga tcgccgcggt ccggccgaag
     8461 aacaccggcg cgtgttcgaa gtcgaaggcc tcgaggccgc gaaacggtga ttcgtgacgc
     8521 caggtcgcga catcagtgcg tgcggcgccg accgcctccg ggaacgtgcg cgcgatcagc
     8581 ttgcgcaggt ggacatcgat cagttcctcg aactgcgcga cgtcggcgaa cggatggaac
     8641 gccgcgcgca gcgtgccgtc cgtctcgtcg tggaaccacc ggcgcacgaa ggcatcgagg
     8701 gcctgcttct gccgcagtcg ctcgagcgac tgcgcatcat cgtcggcatc gagcagcgga
     8761 ggagcggtct ttcgatacac gagcagcgcg ggccgtccgt gccgacggta acccgacagc
     8821 gcgtcctcga actcgaactc ggttcccgat tcgtagtgct tgccgtccgg cttgcgaaac
     8881 gatgccggca ggcgcgtgcc gaggcgcgac cacagcacgc agatggcggc gtgcgcatcg
     8941 gccgggtgcg gcaactggtc ctggaaggag gcggaggcga cgagcggttc gtgttcccag
     9001 aagaccggct cgatccgaac gcgcccgttg aactcgctgc ccaggcggtc gatgacgcgc
     9061 cgggcgatca gccgctcttc ggcgacatcc cccggcgacg acaggaatat ccggaacgtg
     9121 tccaacgcgc gcacattacc gaagaccgcg ccgcggccac aatcgcgccc tgggtcggcg
     9181 gggcgccggc cacgatcaac gagccgcggg ctggccagtg cgccgccgga tcatgagcag
     9241 ggcggcaagt ccggcgagca tcagcaccag ggtgccaggt tccgggacgg gcgcaggcgg
     9301 cggcggtgtc gtgcctccca cgagcacgcc atcgcggaag cggaagaagt aatcggcatc
     9361 ctcgaccagc gcgtcagtgc tgcgaaacag atcggcgccg ccggcggtga tgaactggtt
     9421 gccgatcagc gatgggaagg acgagtcgag ggcgttcgag ccgtagcgca tgtcgacgtc
     9481 gaagtcgccg ttgccgagga agtagatgta cgcctggaag tcgtaggtcg tgtcgggatc
     9541 gtccgcgagc gcgatgttgg tccagaagaa acgtatgccg gccaccgcgg cgtccgcgtc
     9601 aggtgcggcg tcactgagac ccatgccgaa accgaaggta tacgaggtgc tgccatcgcc
     9661 gttcgacgag aaagtgccgt cgccaaggtc gtaggcattc gaggcgagat cggccgcaaa
     9721 cacgccgaac agcggatccg cggcgctgta gccggtggcg gagtcgaaga gcgcgaagct
     9781 gccgtcttcg aacatccgca gcgagtcgta gctggtgccg ccgatattga gggcgtgcga
     9841 actgtagggg ccgccgagca gcgtatcgtc gctgtcccgg tactcggtcg tgaagaggag
     9901 cgacccgtcg gcgggtgccg aggtcaggtc gacgccgtcg tcgaaggagc tgtcgaaccc
     9961 gaccccgggc cggccaggcc agcccgcgcc ggactgcgga aacgactcgg gtccgaagtc
    10021 gacctggacc cgtcgggcct ctgcggtccc gattggcagc agggccacgg caacggcggc
    10081 gatcagggcc ctggacaccc ggttggtgga cattgtcgcg actcccctga agttccgttt
    10141 caggtgccgg caggccgcat ccctgccgac gccggtgaga ggacaagcgc agcaagtacg
    10201 atgccacggg atggacgggc tcgtccgatc aagcagttag tccaggattg gcggcgagtg
    10261 cgattcccgt gtgggaccaa gtgtaagatt cgccgacgcc cgccgggaga atgcgggccg
    10321 gggtatcgcg atggaatgcg gtgtacgcag ctggtcggtg ggcgcggcgg tgctttacgg
    10381 ggccctgggc cttctgccct tgccggcagc cgcccgggag tcctgcgccc tcgacgccct
    10441 cgcatccccg gatctggtca tcgcgaacgt cgagcccgtg cggttcgcga acgacatcac
    10501 ccgcaaggcg atcgcccata cggcgaccga tccggcggcc ttcaagatca aggtgaggcg
    10561 cgcgggtccc ggcgcgagct cgttcaacaa cgagccccgc tacgaactgg cggctctcga
    10621 gatttcgcga ctcctctacc cgccgggtga ggacgtcgtg ccgccgatcg cgctgcgctc
    10681 ggagtctttc cccgccttct acgccatcga ccccgcgggc gtcgaggcgt ggcgcggcac
    10741 caatgccgca ctgttcatgg tgcagtgctg gctcgaaggc gcgaagtccc atcgcctcga
    10801 gatcgacagg tcgcgcttcg cgaccgatcc cgcctacgcg cgcgcgatca gccgcctgaa
    10861 cgtgctcacc tacctgatcg agcacaagga ctcgaacctc ggcaacgtga tgctgactgg
    10921 tgagggcgag accgtgcgcg cctgggcaat cgacaacggc gtcgcgttcg attctgccga
    10981 gagcgatgtc ggcgtgttct ggaggaacct gcgtgtcgac gccgtcgatc gcgcgctcgc
    11041 cgatcgactg cgctcgctca cgcttgagga actgcgcgcg cggctcggcg tgatcgcgca
    11101 gtacgaactg gtcgactcgc gatggcagcc gcgcgagcgc ggcgccaacc tcgatgcgcg
    11161 gcgcggcgtg cgccgtcgtg ccaaccagtt gcagctcggg ctcaccagtg cggaaatcaa
    11221 ttcgatcgag cgccgtcggc aacggctcgt gcgccggatc gacaagggcg agatcagcct
    11281 gttacccgac gcatcctgat gatcgcggtc gccgtggcgc cgtccatgtc gctcgcgacg
    11341 atctcgatgt actcctcgac cagcacatcg cccggcggca cgcctgtgag gcgtgccagc
    11401 gcgccgtcga actcgagcca cggcggcagc ggtccaccgc ccgcgagccg cgcctcgtag
    11461 gcgagcacat cgcccggatc gggatcgaag aacgtctccg ccggcagcac gtggtcgata
    11521 gcccggcccg gatgcacgag cagatcctcg ggctcgcgct ggcgcaccgg cgcggtgttc
    11581 tgcgtctcgg cgcgcgacag cgcgctgtag tggcgaccga tccgcgcggc gacgccgagc
    11641 cagaccacgc agagcgcggc gttcagcatc gcgaagctgc gcgtgccgaa gccgaaccag
    11701 ttgagaccgg caaacaccac gcccgcctgc aggatgtcgc cgaagcgcca gaagaaagtg
    11761 tcgatcgtcg tcttgccgtc gaacttcgcc gctgcgcccg tcggcagata gagcgtctgc
    11821 tccgtcgtgt tcatcagcga atagttggtc gagttctcga ggatcttggc gacgtagatc
    11881 atcgtgaaca caggcacgaa cgcgatcgcg ccgtagacga acagcgcgat cgccggcaac
    11941 accagcactg cgccgcgcac gccgatcgca cggtagacgc gggatacggc gagcgcctgc
    12001 accagcacac cgaccagtgt gacccacagc tggaagcgcg cgtacagcga accgatgatg
    12061 gcttcgcgct cggtgagcaa cgcgtccgcg ccggcgagca tttcggcgcg tgccttgatc
    12121 agcgccgcga gcaggtagtc gccggtcgag ccgatgcagt tgagcagcac gacgaaagtc
    12181 gcgacgagca gcaggtagcg gctgctgaac acgacctgga agccgccgaa taccgagctc
    12241 actgtctttg cagccccggt caccggcacg ggccgggacg attcgggcac ggcgcgcgcc
    12301 tccgggctga tcacaccgat gctcgcaacg agcagcagcg cgccgagcag cagcagcggc
    12361 agtgggccaa ggcgcacgat cgcaagatcg gtgatctgcg cgccggccag cgcaccgacg
    12421 gtggcgccca gcatgatggc cgggaagagc cgctgtccgc tcttggtgtt gaacgagctg
    12481 gcggcgagcg cccagaactg cgcgaccacc atcaatccgt acacactcac ccagatgaag
    12541 aacacgatgc cgaaccactt gctcgcgcga aatggatagg cgacagcgaa ggcgaggatg
    12601 ttcgccgcga agaaggcgct gaccagtcca acgagacggc ggccgtcgat ccggcggcgc
    12661 acggcgctgt agagaggtac cagcagcatc agcaccacgc cattcgccgc gaccgcgtag
    12721 ctgcgcacct cggctgcggc gccagcgagc aggaatgact cgcgcagcgc cttcagcacg
    12781 tagtacgacg ccataatcag gaacgcgtgc gagccgaaca gcgcgatgca gcggccttcg
    12841 ccggcgcgca cgcgggtgag caggcgcaac gcgcgctcga atggcgtgag ttccgcaggt
    12901 gcgctcaatg gacccccgtc gatcgacgat tcccggcgcg aaccgccgac agcttacagc
    12961 cagcgcaccg gcctgctcaa ttgcgccaga acgccggcgt gaacagcacc agcacgctga
    13021 agatctcgag ccggccgagc agcatcgcga gcgagaacat ccaggtctgg aagtcggtga
    13081 acgactgata ctgggtcgtg ggaccggcct ggccgaggcc ggggccgaga ttgttgatcg
    13141 aagccacgac cccggtgaag gccgagacga ggtcgagccc cgagagcagc agcgcgaagg
    13201 tgagtgccac tacggtcgca aaatacaggg aaatgaacgc cagcaccgag aacaccacgc
    13261 gatcgggaat cacgatgcca ccgatgcgga tcggcacgat ggcgcgcggg tgcagcagca
    13321 tcttcatttc gcggaacgca tggcgttgca gcagcagggt gcggaacatc ttgataccgc
    13381 cgccggtcga gcccgtgctg cacacgatgc acgagaggaa cagcaaccat accggcgcga
    13441 acaccggcca ggcctcgtag ttgtcggtga cgaagccggt cgtcgtcgcg atcgacacca
    13501 cggtgaacgc ggcgtgccgc agcgaggcgc cgaaaccggc gtaggtttgc gaggcccaca
    13561 gcagtgccgt gatcgcggcc acgctgacac cgagcaccag catgatggcc ttggcctccg
    13621 cgtcgcgtgc gtaaggcttg agtgaacgcc gctgcaccac catgaagtgc cgcgagaagt
    13681 tcacgcaggc gaacagcatc agcgcgatca gcacgatctc taccgccgcc gaatcgaaat
    13741 gcccgatgcc gctgtcgtag gtcgaaaagc cgcccagcgc catcgccgag aacgcgtggc
    13801 agatcgcatc gagccagttc atcccggcga gtcgcaacgc cagcacgccg accagcgtga
    13861 cggtggcata gaccagccac agcgccttgg cggtctgggt gatgcgcggg gtcagcttct
    13921 cgtccttgac cgggcccggg ctctcggcct tgtagacctg catgccgccg acaccgagca
    13981 gcggcaggac ggcgacggcc agcacgatga tgccgaggcc gccgtaccag tgcagcgcgt
    14041 gccgccacag gttgatcgac ggcggcagcg attcgatatg ggtgagcacg gtcgcacccg
    14101 tggtggtgag ccccgacatc gtctcgaaga aggcgtcggt gaccgacagg tcgggcaacg
    14161 ccatcagcag cggcaccgtg gcggccgccg acatcatcac ccaggcgagg gtcacgagaa
    14221 ggaagccgtc gcgcgccttc aattcgcgct tgtgacggcg gaacgccagc gcgaggccga
    14281 ggccgaccag cacgatgacc gtggcgccga cgacaaagtg gaccagcatg ccgtcgccgg
    14341 tcagcagcga gcaggcgatc ggcagcagga aggtgctcgc gaacagcgcg agcatcaggc
    14401 cgagtacgta caggacgccg aacacgcggc gttacgggct ggtgccggac acgaacagtt
    14461 tctccacctg gtcgacgtgg cgacggtcgc cgagaaagat gatcacgtgg tcgttttcct
    14521 gcaccaccgt atcgtccttc gccatgatga cgtcgttgcc gcgcgtgatc gcccctatgg
    14581 tggcgctgtc cggcagcggg atgtcgccaa ccgcgacacc gacgatgcgg gagctgcccg
    14641 ccctgccgtg cgcgatggcc tccatcacct cggcgctgcc atggcgcagc gaatgcacgc
    14701 gcaccacgtc gccgcgccgc acatgggcga gcagcgagcc gatcgtcacc gtctgcggcg
    14761 agatcgcgac gtcgatgctg ccgctctcca tcagctcggc gtaggagggc ttgttgatca
    14821 gcgccatgac cttccttgcg ccgaggcgct tggccagcat cgccgagagg atgttggctt
    14881 cctcggagtt ggtgaccgcg gcgaacacgt gcgagttgtc gatgttctcc tcgatcagca
    14941 gttcctcgtc cgcggcgtcg ccgttcagga cgatggcgct ctcgagccgt tcggcgacgt
    15001 acctcgcgcg atcggcattg cgttcgatca ccttgacgtg gtggctgtct tcgagcgcgt
    15061 gcgcgaggcg caagccgatg ctgccgccgc cggcgatcac gatgcggcgg gcggcctcgc
    15121 ccgcgacgcg cagttcatcc atgacgcggc gcatatgctc gcgcgtggcg aacaggaaca
    15181 cttcgtcgtc ggcctcgatc accgtttcgg gcccgggctc gatacggcgc ccgtcgcgat
    15241 acacggcgac gatatgcgag tcgaagtccg gcaggtgctc gcgcagcatg cggatcggct
    15301 ggccgacgag tggcgcgccg gtctcggcac gcacgccgac gagccggacc cgacccgccg
    15361 cgaaggtcag cacctgcagt gcacccgggt agcggatcag gcgctcgatg tactcggtga
    15421 ccagctgctc ggggctgatc cagacgtcga cccgcagggc ctcggcgctg aacagcgcgg
    15481 gcgtgctggc gtactcggac gagcggatac gggcgatctt ggtcggcgtg cgatacagcg
    15541 tgtaggcaat ctcgcacgcg agcatgttga tctcgtcgct gttcgtgagc gcgatcagca
    15601 cttcggccct ggcggcgccc gcagcctcga gtaccgacgg gaacgagccg ctgcccgcga
    15661 ccgtgcggat gtcgaggcgg ccctgcaggt cgcgcagcac ctgttcgttc gtgtcgacga
    15721 ccgtgacgtc gttcgcctcc tcgcgcgaca ggtggtatgc ggccgtacga ccgacctggc
    15781 ccgcgccgag aatcacgatt ttcatgttgt cacatggcct cgaggttagc gtacgcgagc
    15841 atgagccatt tcgtgccctg gcgctcgaag ttcacctgta cgcgtgcctg cgggccctgt
    15901 ccctccacat tgagcaccgt gccttcgccg aacttgccgt ggcgcacgcg tgcgccgagg
    15961 cgcatgcccg gcgccaccgg ctccgcggcg atcgcgcgcc gtcccgcctg gccgggagac
    16021 cacgccgcgc cgccgcgatc cccgtgggtg ggcgcagggt gacccgagcg cgcataagga
    16081 cgaccgagct ggatgcgcgg ccgcacttcc tcgacgagtt cctcggggat ctccgccagg
    16141 aagcgtgagg cctggccgta gctgtccatg ccatgcaggc ggcgttgctc ggcataggtg
    16201 acgtacagct gctgcatcgc gcgggtcatg cccacgtagc acaggcggcg ctcctcctcc
    16261 aggccatcga ggtcgttgag cgagcgctgg tgcggaaaca ggccatcctc catgccgcac
    16321 aggaagacca ccgggaattc caggcccttc gcggtatgca gggtcatcat ctgcacgcag
    16381 tcttcccatt cctcggcctg gccctcgccc gattcgaggg cggcatgcgc gaggaaggca
    16441 tcgagcggcg gcagatcgcc ctcgggttcg aagccgcgcg cggcgctgac gagctcctcg
    16501 aggttctcga cgcgcgcctc gccgcgatcg gccttttcct tgcggtggtg ttcgacgagg
    16561 ccgcttgctg cgagcacgag gtcgacctgt tcgtgcagcg gcaggttcgc ggcatccgca
    16621 gagagccgct cgatcagctg cacgaagccg ccgagcgcgc cagtggcccg tgcggcgagc
    16681 gtgccgtcga cgatcgcctg gcgcgccgcc tgccacagcg agatgcccgc cgcgcgcgcg
    16741 gcgacccgca gctcatcgac ggtcttcgcg ccgatgccgc gcgccggcag gttcacgacg
    16801 cgctcgaacg aggcgtcgtc gtcgcggttc gagagcaggc gcaggtaggc gagcgcatcc
    16861 ttgatctcgg cgcgctcgaa gaatcgcagg ccgccgtaga ccttgtacgg aatgcgcgcc
    16921 gagaggaacg cttcctcgaa cacgcgcgat tgcgcgttcg agcggtacag gatcgcgata
    16981 tcgcgccgtg cgccgccgcg ctgcacccaa tcgcggatgc ggtgcgtaac gaagtcggcc
    17041 tcgtcacgtt cattgaaagc ggcatagagc cggatcggct cgccgtggcc gccgctcgtc
    17101 cacaggttct tgccgagccg gccgctgttg tgcgcgatca gctcgttcgc ggccttgagg
    17161 atgttgcctg tcgaacgata gttctgctcc agccggtgca gtatggcctg cggccagtcg
    17221 cgctggaact gctgcaggtt ctcgacccgc gctccgcgcc agcgatatat ggactggtcg
    17281 tcgtcgccaa cgatgaacgg cgcgccctcg ctgccgacca gcagcttcag ccacgcatac
    17341 tgaatggcgt tcgtgtcctg gaactcgtcg accagcacgt ggcggaagcg cgtgcggtag
    17401 tgccgcagca cctcgacgtt gtcgcgcagc agttcgaacg cgcgcagcag cagctcggcg
    17461 aaatcgacga cgccggcgcg cgcgcaggct tcctcgtaat cccggtacag cttgatcagc
    17521 tggccgcgtg tcgggtcgtt gccgtccttg agcgccttcg ggcgcaggcc ctcgtccttc
    17581 tggtgattga tgaaccaggt gacctcgcgc ggcacccagc gcgactcgtc gagcccctgc
    17641 gcgcgcagca acttgcggat catgcgcagc tggtcctcgg cgtcgaggat ctggaagccc
    17701 tgcggcaggc cggcttcgcg ccagtgcagg cgcagcaggc ggtgtgcgat gccgtggaaa
    17761 gtgcccaccc agagcgaact gccgggaatg ccgagcaggg cctcgatgcg cgcgcgcatc
    17821 tcgcccgcgg ccttgttggt gaaggtcacc gccaggatgc tgtggggcga ggcgccttcg
    17881 gcctgaacca gccaggcgat gcgatgggtg agaacacgcg tcttgccgct accggcaccg
    17941 gcgagcacga gtaccggccc gacaggcgcg gtgaccgctg cacgctgcgc gtcgttcagc
    18001 ggcgagagaa tcggggagag gtccatagag ccgcctatta tgccgggggc tccgaccttt
    18061 catccatgta gtcgagcagt gcggcggcga gggtcagcac gagggggccg acgatcaggc
    18121 cgatcgcgcc gaatgcggct gctccgccga gcacgccgat gaacaccgtg agcgtggaga
    18181 tcctcgcccg gctcgacacc agcagcgggc gtaccaagtt gtcggccgag gacacgccga
    18241 ggccccatat cagcaggaag atcgccatgc cccagcggcc ctgcatcgcg agatagagca
    18301 cgccggggat ccagacgatc gcggcgccgc cgaaaggcag cagggcgaac agggccgcga
    18361 gcacgccgaa caccacgaag gaggacaggc ccgcgatcgc gaagccgacg cctaccgcgg
    18421 cgccctgcag caacgccgtg atgccgctgc cgaatacgac cgcgcgggtg acctgggcga
    18481 ggtgatcgac caggccgtcg cgccgctcgg gctgcatcgg gatgagccgc acgccgcgcc
    18541 tgatccaggc ggcaccgtcg cacaggaaga aatagagcag cacgatcgcg atcgcgaaag
    18601 cgagcacggt gccgagcgca ccgacgacca cgttgccgcc gattgtcgcg ccccacttga
    18661 ggaacgactc gccgctcgag agcagccagt cgcgcagctc ctgggcgccg aaaccggtgt
    18721 gctcatcgac ccacagcagg accggtccga cgaccggata ttgctcgaac tgggtcaggc
    18781 cgcccacgcc gaggccctgc acgaagcccc gggcacggcc ggccagctcc gcagcctgcg
    18841 ccgcgaacac cccgccgatc acggcgagcg gtatcaggat ggcgacgggt gtcaggaccg
    18901 tgatcagcgc ggccgacatg gtcgcgcgcc ccttgaaaca cctggtccag cgctcgtgca
    18961 gcgggtagag catgaaggcg aggaacgccg cccagaccat cgggccgagg aaaggctcga
    19021 gaacccggaa cagcagataa ccgacgagca gcagggcggc gactgcgaac ccgcgccggt
    19081 agaacgggga gtccatcggc gcattatgct atcggcagga ggccttggcg atgaagctct
    19141 acgactatct cgactcgggc aacggctaca aggtgcggct ggtgctggcg cagctcggcc
    19201 agtcctgcga ctgggtcgaa tgcgacatcc tccagggcgc cacccgtacg ccggagttcc
    19261 tggcccgcaa cccgaatggg cgcataccgc tgctcgaact cgacgacggc agcttcctcg
    19321 ctgaatcgaa cgcgatcatc tggtatctcg ccgaaggctc gccgctcgtt cccgacgatc
    19381 gcctgctgcg cgcgcaggtg ctgcagtgga tgtttttcga gcagtacagc cacgagccgt
    19441 acgtggccac gccgcgcttc atcgtcaggc acctgccggc agattcgccg cgccgggcgg
    19501 aattgcccga gcgcctggag cgcggccgcg ccgcactcgc cgtgatggac cgccacctcg
    19561 gtggccagcg cttcttcgtc ggccatcgct acacgatcgc cgacgtcgcg ctgtacgcct
    19621 acacgcacgt agcccacgag ggcggccacg acctcgcccc gttcgcgcac gtgcgcgcgt
    19681 ggctcgatcg ggtgcagtcg cagcccgggc acatactgct tgcagcgcgg ccggcgccac
    19741 gccgcgcctg agcgcagtta ccagcggttg cgcaactcgc gcagcttgag gaagacgtcc
    19801 cgcgggctcg gctggtccgg cagatcgggg caggcttcgc gcagccgggc gacgatctgc
    19861 gggttctgca gccggaagaa cgtgttgatc cgcttctcct cgccgagcgt ggtgatcggc
    19921 gcagccgccg ggtcctggac ctgcgcgtcg cgatgcatcg tcgcggcgat ctcgttaccc
    19981 ggctcgcgat ccagcgtgaa tgccaggttg cgctcgagat attcatggcc cgggtatata
    20041 cgcgtggcgt cgggcagacg ggcgagctgc tgcgcgaatg tctcgtagag cagctcgggg
    20101 tgtccgccgt ggtggcagtt gccggcgccg gcattgaaga gcgtgtcgcc gcagaacagc
    20161 accggcgtct cgccacgccc gtagaggcac aggtgcgcga gcgtgtggcc tggcgtatcg
    20221 aggcactcga gctccacggt gcgcccgacg cgcaccacgt cgccgcgcgt gagtccgacg
    20281 tcgacatggc cgatgcgagc ggccgccccg gcatgcgcca ggacgcgcgc gccggtggcc
    20341 tcgcgcagcg gctcgttgcc acccgtatgg tcgcggtgtt cgtgcgtgtt caggatctgc
    20401 gtgatctcgt aaccgcgcgc gcgggccgcc gcgacgcact tgcgccagtc gagcgggtcg
    20461 atcgcgagcg cctcgccggt ttcggcgcag gcgacgaggt aatggaagtt gcggtagtcg
    20521 ttcgcggtcc agatccgttc gatcagcatg tccctacccg cccatgcccg tgcgcccgat
    20581 gacgcgatga tcgtggtcgt cgggctccag cggggcggtg atgcgcacgc gcgtgccgcg
    20641 gcccgggctg gagtcgatct cgagcgtgcc accgatgatg gccaggcgct cgcggatgct
    20701 gtagagaccg aagcccccct gctctgtagg caggaaggtc aggtccgaga cgttgaatcc
    20761 gacaccggaa tcggaaattc cgagttcgaa ctggccgtcg gctacccggg tgcggatcgc
    20821 aacgaccgtc gcctgcgcgt gccgcgccac attctgcagc aactggcgcg cggcctggta
    20881 gatgagcacg gcggtcgact cggcgagggg cttttgcgcg ccatcgtcct cgaactcgac
    20941 ctgcacgcgc tggcgcttgg cgaactcctc ggcgagccag cgcaggccgg agacgatgtc
    21001 ctggtcggag agcccgggcg gcatcaggtc cgagagcagc gcgcgcacct gggcggtgga
    21061 ttcgctgatc gtcagttcga gcgccgagag gtccggcagc ggccgaccct gttcgacagt
    21121 ggcgcggatc gccgcgacgt gcatctgcag gccgatcagg ctctgcgcaa ggccatcgtg
    21181 gaggtcggcg gcgatcttgc gccgcgcgcg ctcctcggtg agcgtcagtt cggtggcgag
    21241 cgcgcgcaac ttgcgctgct gctgctcgag gcgccgctgc gcggcgcgat agtgcgtgat
    21301 gtcgcgcgcc actccccaca ggcgccgcaa ggcgccgtgg tcgatttcgg tcgtgagcga
    21361 cacgagcagt gtgcggtcga gtccataagg gcccggcagc acgcattcga ggttctcgac
    21421 acgtccgccc gcgatgatcg cgtcgggcac cgttgcgagg cagtgcttga gccacggcgg
    21481 atggcgcgtg atgcgatccg tcacctcgcc cgtgccttcc gccttgcgat aggcgtcgtt
    21541 ggcttcggcg atccaggcat acttgtcgag ccactggcgc tgctccttcg cggacagcag
    21601 cagcggcatc ggttgcgaca gctcgagccg gaagatcgcc tccgaactct ggctgacgaa
    21661 ggcgcgatag cgctcgtcgc tcgcgcgcac gagctgcagg gcatcgcgct tctcggcgat
    21721 cgacgcctgc accaccagca ggggcacgac gaccagcgcg aggaatgcct gcaggttcag
    21781 tgttgcaccg aacggctcgc ctggcgtcac ggcgaacggt ccccttccgg cgagcgtgca
    21841 cgtggccgat acgagcgcga ccaggaagag cgccagcgtg atgcgccgcg ccccgcagtt
    21901 cgccgagatc cagagcagcg cggcatagac gacatacggc gaatccagcg cgaatgtctc
    21961 gaacctgtgc tccatgccga acacgaacca cagcagcagc gccagcaccg tgaactgcag
    22021 cagcacgtac caggcccgcg gcggcggctg ggccggcgcg gcgcgcacgc ccgactccgc
    22081 ccaggacacc gccagcggca cgatgatcac cgcgccgacg tagccgctga accaccaggt
    22141 ctgctggttg ctccagtacg ccgctgtgcc ggccgcagtg gctacggcgg cgccgatcac
    22201 gccgccgacg aaggtggcga cgaggagtat gaaggtcagg cgcgcggtcg ggccggtgcg
    22261 ggaaaaatcc acccgcgggc cgatatggcg cccgatgagc caggccgcga ataccgcctg
    22321 gccgatgttc accaggccga ccagggtacc ggcgagcgga ttgaataccg aatggacgag
    22381 cgcaaacgcg cagacctcgg cgagcagacc cgaggcgaac agccatggcc agtaacgccg
    22441 cggaaagagc atcagggcgg cgatgtaggt accggctccg ggccagatca ccgcggcgct
    22501 gtgggcgccg atcatcaaat ggctgcctag ccaggcggcg gcgaggtgta cagggaaaaa
    22561 tcctgccgcg accgcgagcg gcggccactt cgccaggaac tgtcgcggct cgtcatgcac
    22621 cggtcggcct cgcacgcctc gtgccggtca ccttgccggt gtggccgatc cgcgtcaact
    22681 gcgcagcgag tcggcgatca gctgggtcgt cggcaggtag tggtccacca gcagcgcgat
    22741 gaagatccac atgagccagg tcacggagaa cttgaatacg cgcatcggca gctcctgccg
    22801 gtcggtgaat ttcatcgcga gcgcgtagta gaggaagatg ccgttcagca ccagtgcggc
    22861 gacgagatag atcaggccgc tcatgccggt gacgaagggc atcaatgtca cgagcaccag
    22921 cagcacggtg tacagcagca cctgcaggcg cgtgtacgcc acgccatgcg tgaccggcag
    22981 catcggaatg ccggcgcgcg cgtactcgtc cttgcgggcg atcgcgaggg cccagaaatg
    23041 cgggggcgtc catacgaaga tgatcaggaa cagcagcagc gcgtccgggt cgatcgagtt
    23101 ggtcactgcg gcccagccga gcaccggggg agccgcgccc gccgcgccgc cgatcacgat
    23161 gttctgcgag gtcgcccgct tgagccaggc ggtgtagatc accgcgtagc cgatcagcga
    23221 gaagaaggtc agcacggaag tcagcggatt gacgaggatc cacaggatca gcatcgacag
    23281 gacgccgagc gccgccgcga acagcagcgc gcgtgcggtg ctgatcttgc ccttcgggat
    23341 cggccggttg cgcgtgcgcg ccattttctc gtcgatcttc tggtcgagca cgtggttgat
    23401 cgcggcggcg caggcggcgg ccagggcgat gccgaggttg ccccacacga gcggcccgag
    23461 cggcggccag ccgggactcg cgagcagggt gccgacgatc gccgtgaaga cgatcaggta
    23521 gacgaccctg ggcttggtca gctcgaagta gtcgcgccag ctcatgcgcg tacgcgccgg
    23581 ttgagcagca ccgccgacag cagcaacaac gcggctccgg cgttgtgcgc ggtggcgagc
    23641 catagcggga agccccgcca caccatgaag atgccgatca ggagctgcaa tgccagggcg
    23701 gcgatgacga ataccgcggc acgcaccgtg cccggcgtcc tgatgcgcgc gagcgccgtg
    23761 gcggcggcga tcagcagcgc gatggaggcg acgatcgcgc cgaggcggtg cacgaagtgg
    23821 atcgcgacgc gggtcgggtg atccagcacg ccaccctcgt agttgatgcc gagtccgtgc
    23881 cacaacacga acgcatcgcc aaaatccatt ccaggccacc agcggccctg gcaggtcgga
    23941 aagtccgggc acgccatcgc ggcatagttg ctgctggtcc agccgccgag cgcgatctgc
    24001 agcgcaaggg cgacgatcgc gatgatcgcc cagcggcgca cgaggcgcgg cgcatcgtgg
    24061 ttctgcgggc ggggtcgcag cgacagccac agccaggaca gcatcgcgag cgtggtgagg
    24121 ccgaatacca ggtgtgcggt cacgatcagc ggtttcagca gccaggtcac cgtgagcatg
    24181 ccgaggatgc cctggaacac gacgacggcg agcagcgcga ccgacagccg cagcggcacg
    24241 agccttcttg cgcgcgtggc gatcgcgatc gccgtgagaa ccacgatgat gagcccgagc
    24301 gtcgtcgcag catagcgatg gatcatttcg cgccacgcct tgcccgcgtg gacttccgcg
    24361 ccggggaagc gctcctgcac ggcggcgtcg ttggcgaggg cgccgatcgg ggtgatgtgt
    24421 ccgtagcagc ccggccagtc cgggcagccg agacccgcgt cggagaggcg cacgtaggcg
    24481 ccgaggacca cgacgatgag gcacagcagc atggccgcga gtgcgagccg gcgggcgagt
    24541 gtggtgacgg tcaccgagcg aggtccttca gccgacatgg gatagcccca gcagtttctt
    24601 cagatcggtg agcaggccct tgggatcgtc atgcgagtcg aatgtcatga tcaggttgcc
    24661 gagcggatcg atgaggtaga tcgtgttgtc cggcgtaccg gccggcagcg cgccgagcag
    24721 cgcactgccg gcgctgccgg ccgggtcgag tgtgatgatt cccggatgtt cgcttgcgag
    24781 gaagtccgtc tcgcagcaat ccgccggcgc gatgaacacg cgctgcacgc gatcgaaatc
    24841 cttcgccagc agcaggtggg tctggcgggt cttgtacagg gcctcgcggc agctgtcgct
    24901 gcaggcgtcg ctcgcgacga gcagcagcgt ccatttcgat tccggccacg gcacttcgag
    24961 cgccacggga tcggcgagca gcacgccgtg gttggtgctc tgcgcgggcc gccagccgcc
    25021 gccgtagtag agcgcgaagg cgatgaacag cggcacgaag aacagcgcgg cgaggccgat
    25081 caacgtacga agtccgcggg cattggcgtt gttcatcgat ctgtcttgtg caggttcagg
    25141 atcacgtaga gcaccgcgac ggtcgcggca aacagccacc attgcgccgc gtacgaaaaa
    25201 tgacgttccg gcggtatgcc gggtgcctgc cattggcgaa tgtagccgtc cggcgcatcc
    25261 gcatcgagca ggacctggcg ggtctccaca gcccgcccaa gcaccgcgac gagttcgtcg
    25321 tgccgaggat agctcgtaag cttgggccac gatgcatccg gatccggcgc cgcgtggccg
    25381 gcggccatgc cctgcaccgg cagcgtatca acgcggccag tcacggtcac ggaccccgtg
    25441 gtgccggcca gtgcgacatc cggcaagcgg tcgcgatagc cggtgaacgg cacccaaccg
    25501 cggttgacga ggaccaggcg gccatcgtgc agttcgaacg gcgtcagtac ctcgtagccg
    25561 gcccggcctg cgtgcgtgcg gttgtcgagc aggatctggt gctcgccgtc gtagcgcccc
    25621 tcgagccgca cgcgcgtgaa gcgcggcagg gcctcgagtg cctgcatgcc ggggtcgatg
    25681 gtcggcgcag cgtcagtcgc gaaatcctgc cacacggctt gcctcgcgac gccgcggttc
    25741 cattgccagg caccacaagt cacgaacaca gcgatgctga gcactgccag tgcggtcatg
    25801 ggccaggagg gcgtgaacac ccgccggcgg ccgatgtgga tcgatagcct caaatggcgc
    25861 tacccggggg gaaatggccg cgaattgtgg ggtctgggtg tcgggaagta caggggcgcg
    25921 ccgcctgcgg cggccctata atcgccccca catggagctc atccagatct tcgtcgtggt
    25981 cgtgctgatc gcgatcgtcg caagcctcgg cagcgcactg ttccacctgt catcgcccgg
    26041 tcgcgacccg aagaagatgg tgcgtgcgct gacctggcgc attgcgttgt cggtctcgct
    26101 tttcctgctg ctgatgctcg cctggcagct ggggtacatt acgccgcacg ggctctagcg
    26161 cggcaccgtc tcagctgcgg atcgtcgtgc ccgcgcgccc cgcgaggatg ccggcgacat
    26221 cgtcgagcga accgacgcag gcacgccggc caggcccgct cgtcgcaaag cgccgcgccg
    26281 cctcgacctt cggtcccatg gaacctgccg ggaacgtgta ttgcgccagt tctccgggcg
    26341 tcgcggcgcg cagctcgcgt gcctgtggcg tgccccagtc gatgaacacc gcgggcacgt
    26401 cagtcagcat cagcaggcag tcggcgtgca accgctcggc gagcagctcc gacgtcaggt
    26461 ccttgtcgat cacgcactcg atgccctcgt aggatcccgc ggccgagcgc cccacgggaa
    26521 tcccgccgcc gcctgcgcag atcacgacat agccggcgcc gagcagcagc tcgatggcgc
    26581 ccagttcgag tatgtccacc ggttcgggcg agggcacgac gcggcgccag tgcccgccat
    26641 ccggcgcaat acgccagccg cgctccgcgg cgaggcgctg cgcttcggtc gccgcataca
    26701 ccggaccgat gaacttggtc ggccgctcga acgcgcgatc ggccggatcg acttcggtct
    26761 gcgtgagcag tgtggcggcg cgccgttcgc cgagctcgtt catcagctcc tgctcgagca
    26821 cgtagccgat catgccctcg gtctgcgcgc cgaggacgtc gagcggatag gaagcatcgc
    26881 cggccgcggc cgactgcagc gcaagcaggc cgacctgcgg accattgccg tgggtcacga
    26941 ccagctcatt gcccgcggcg accgtggcaa tcgacgccgc ggcgcgctgc gcattgcgcc
    27001 gttgcgtcgg gacatcgagc ggttcgccgc gcttcaggag cgcattgccg ccgagcgcga
    27061 cgacgatggt cttcctgctc atgtcgaacc ttccttgt
//
LOCUS       DAOQVY010000024        26124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_2185_length_26124_cov_5.028309, whole genome shotgun sequence.
ACCESSION   DAOQVY010000024 DAOQVY010000000
VERSION     DAOQVY010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 26124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26124
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2185_length_26124_cov_5.028309"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1552)
                     /locus_tag="PKN91_03875"
     CDS             complement(173..1552)
                     /locus_tag="PKN91_03875"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS27242.1"
                     /translation="MRLTFHGAARQVTGSCFAFEVGHSRFLVDCGLFQGARATREANT
                     APFAFEPAELDFVVLTHAHLDHSGLLPRLAAEGFSGPIYATAATRDITAVLLRDSAYL
                     QQVEEERSRRRGGDYVAAYSMADVDAVLPLIRTVDYEDIFEPGTGVRVRLRDAGHIIG
                     AAIVELWLDDGVSQRKVVVSGDLGQPGRPIVRDPTAIADADVLLLESTYGDRDHRPYT
                     ATIDELVETLERALYKKKGMVLVPAFAVGRTQEFLYILNGLARAGRLRDLDVFVDSPM
                     ATEVTHITARHFDLFDAEARRAAQAPPGDGVAPRVRFIETVDESKSLNRLTGGAIIVA
                     ASGMCDGGRIRHHLRQHLPFPRTTVIFIGYQAAGTLGRRIVDGADVVRISGEEVPVRA
                     EVATLGGFSAHADRTALLDWLAKFKRPPAVTWLVHGEEHASRALAAGIESRFHCDVRI
                     PSDHETVVL"
     gene            complement(1563..2243)
                     /locus_tag="PKN91_03880"
     CDS             complement(1563..2243)
                     /locus_tag="PKN91_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27243.1"
                     /translation="MDFYITTHGVLRWLHVLAMVYWLGGEWGVFNTARYVTKVNLSLD
                     ERRRHMDTAFRIDILARLGIILLLPLGLHMGYNLGVQPLGGPWIVGTWIFFGAWLCLT
                     LSAYYTHQSDLGLRLTRIDEALRYVLIPALLIPGVMGLFGAGPLGARWYAFKVTLYAI
                     LLIIGLVLRVVMRHWVTLFRSLESGASRSAVEAQLAKEIALSRILAYFYWVGIATVAF
                     FGVLKPIA"
     gene            2260..2874
                     /locus_tag="PKN91_03885"
     CDS             2260..2874
                     /locus_tag="PKN91_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS27244.1"
                     /translation="MDDRANHGRLVCARTSVNTRIRRPFVKPHLAVSLGLKAIEAGYR
                     VVFTTAAHLIAVLAKTHAEGRLDEKLKVFTTPRLLIIDEIGYLPIDRLGANLFFQLIS
                     RRYERGPMILTSNQSFGAWGEVFGERTIATAILDRLLHHAVTLNIRGNSSRIFSASSS
                     FSLRSCEISRPLHLAFTPASCALAAGGDATRSTCRRRSACVTTP"
     gene            2936..3187
                     /locus_tag="PKN91_03890"
     CDS             2936..3187
                     /locus_tag="PKN91_03890"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNS27245.1"
                     /translation="MPEAASPSTAHIFDARGVARRFRHSAIFGALGALQPGEAMRFVN
                     DHDPLPLLDQLRQRFGEHIAIEYQQREPGAIVIDFTIVA"
     gene            complement(3286..4698)
                     /locus_tag="PKN91_03895"
     CDS             complement(3286..4698)
                     /locus_tag="PKN91_03895"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HNS27246.1"
                     /translation="MTTPAERPRPRPGRRSWAEPWKVKVVEPIRMTTPAERARALAEA
                     GYNTFLLRSEDVYIDLLTDSGTSAMSDWQWAGMMLGDEAYAGSRNFYHLEETVRKYYG
                     YRHVVPTHQGRGAEHLLSTILVEPGDYVPGNMYFTTTRAHQELAGGTFVDVVIPEAHD
                     PASEHAFKGNVDLGALEALIAKVGAQRIPYVCVAVTVNMAGGQPVSMANLRAVRELTQ
                     RHGIRIVLDATRAVENAYFIQQREDGYRDRKVADILHELCSYSDACTMSGKKDALVNI
                     GGWLAVNDTGLYEDASNLVVLYEGLHTYGGMAGRDMEAMARGIVESVDDEHIKARVGQ
                     VEYLGRMLIDMGVPVVRPIGGHAVYLDAKAFYPHLPQEQFPAQALAAYLYEDAGVRAM
                     ERGIVSAGRSKETGEHHHPALELVRLTIPRRVYTQAHMDIVAESAAAVFDARDSAVGL
                     RMVHEPRYLRFFQARFERLA"
     gene            4796..5698
                     /locus_tag="PKN91_03900"
     CDS             4796..5698
                     /locus_tag="PKN91_03900"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNS27247.1"
                     /translation="MRLDLDAMEALEAVVRCGGLARAAEELHKVPSAVSYQLRRLEQQ
                     LRVALVDREGYRVRLTPVGEVVLAQGRRLLAQARQVESLARQFADAWEPRLLVIVDGI
                     LPVKPTLTAFNALSSEGAPTRFQVKVEFQRGVQFCFERDDADLMVVKDYDPKPYLVAK
                     ELAAVECILCVGRRHPLAAQRSVSLEDLQDYVELSVQDSSGKRDDPHGFGGERVFHLS
                     GFDAKKQALLMGAGFGWMPHGMVRAELRAGRLRELRYAGGSRYRFTPRLVHRLDRPPG
                     RAGRRLAQLLREASGKDGDLIGHD"
     gene            6075..8558
                     /gene="mgtA"
                     /locus_tag="PKN91_03905"
     CDS             6075..8558
                     /gene="mgtA"
                     /locus_tag="PKN91_03905"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HNS27248.1"
                     /translation="MGEQAWWATGIADLAKRLGSGPEGLGSAEAGTRAQGRPRRSRGG
                     QALRLFLSQFTNPLVLILIAGAVLSLFLREWVDAAIILVIVIGSGLLGFTQEHAASRA
                     IEDLRQRLVRRVSLLRDGKEARIPLDEVVPGDVALLQAGDLVPGDGRILDSAALLVDE
                     AALTGEPFPVEKSPSIAPESATLMQRTGALFKGTSIRSGSCRLLVVTTGKDTVFGSIE
                     RDLERAEETTEFARGLTGFGNMLMRVMLVVVAMVLVAATLLGRDLVTSILFAMALAVA
                     ISPELLPAIVSVTLAAGARRMTRHGVLVRRLQAIENLGGMDILCTDKTGTMTVGSIAL
                     SGAVDTSGQASGDVLRLAAINARLQTSMASALDQAILAAASAASLASEISGKLSEIPY
                     DFVRRRFSVAARLTDGTEVFVTKGAVNEALSTCTRARYGDGVHALDAGGRDTLLSFVR
                     SKAESGFRALAVATGDEGEACLEGFLLFLDPPKPGIRDTVAAMASRGISVRIVSGDNR
                     HVTARMAGVIGLEPGEVLTGDEIAALDDDALAARIGGVRVFAEVEPQQKERVVRAFRK
                     AGHAVGYMGDGINDAPALRAADVGISVDSAVDVARESADIVILRPDLGAIQCGVEEGR
                     RTFANTLKYVSITISANFGNMVSMAFATVFLPFLPLLPTQILLNNFLSDLPAIAIAGD
                     AVDRERMASAQRWDIRRIRNFMIVFGLISTVFDLLTFAFLWLVMRADPDTFRTSWFIV
                     SLLTELAALLVLRTRRRFWRSPPSRLLVWSSVLVAVLSLALPYSGEIAGLFAFRPLAP
                     ALVGLLLLIFAGYALVTEFAKRRYGIQAH"
     gene            complement(8705..8789)
                     /locus_tag="PKN91_03910"
     tRNA            complement(8705..8789)
                     /locus_tag="PKN91_03910"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8753..8755),aa:Leu,seq:cag)
     gene            8845..10893
                     /gene="rnr"
                     /locus_tag="PKN91_03915"
     CDS             8845..10893
                     /gene="rnr"
                     /locus_tag="PKN91_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNS27249.1"
                     /translation="MKRTRKPSQKDRGARGAHPTQRRQGQRAAGQAGHKRAAVKAATG
                     PMAKGIVEGVVSAHRAGYGFARIEGMTDSAFIPPFAMAGLMHGDRIRLTVKRDATGRY
                     TGEVLGIVEHGVKAFLGTIDVEGRAAYVLSADRRLALRCQVAAADLNGAKHGDWVIAV
                     VTRYPGAKGGAHARIERRLDPDRPVELATETAIARFGLPTDFPAEVRAEAEACGQHVD
                     PREAAQRVDLRALPLVTIDGEDARDFDDAVYAEPHEHGFRVVVAIADVSHYVRQGSAL
                     DIEAAKRGTSVYFPLRVIPMLPAALSDHLCSLQPKVDRLCFAADMVITKQGQLKYARF
                     YPAVMHSAARLTYTLAYEALFEGRPEARKAIGPLIERLLPLVDVYRALHKARNRRGAL
                     DFDAPEATFDIDSQDRVKGIVLASRNDGHRLIEECMILANVTTAHELARQRVGSLYRV
                     HGKPDEEKLDRLLSTLAALGFPAEIPETVTPRDIRQITHRLGASPARPFVESLVVRSM
                     PQAVYQSANIGHFGLALTGYAHFTSPIRRYPDLVVHRSLKAMLGVPAPIGVRYGAEEL
                     ASLGESTSRLEKRADEADRYVDQFLKCSWLRERLGQTFEGLVTAVVEFGCFVQILEIS
                     ADGLLHTATLVDDAYELDAGGHAWVGKRSKRRLGLGERIRVIVTNANPVEGLIDLELA
                     "
     gene            10890..11639
                     /gene="rlmB"
                     /locus_tag="PKN91_03920"
     CDS             10890..11639
                     /gene="rlmB"
                     /locus_tag="PKN91_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNS27250.1"
                     /translation="MSATERLYGLHAVRAVLTRDPSRVTQLTLTHQRDDKRIAALEAL
                     ARDAGVAVRRVDAHALAGLVGDVVHQGVVADVSPLKSWSEDDLIDALREVKAPLVLAL
                     DGVQDPHNLGACLRTADACGALAVVVPKDRAAQVNATVRKVAAGAAETTPVVAVTNLA
                     RTLRLLKAAGLWIVGAAGEADKVAAGVDLTGPLAVVMGAEGSGLRQLTRQNCDFLVRL
                     PMLGAVESLNVSVATGMLLYEVIRQRTHAGA"
     gene            11746..12141
                     /gene="rpsF"
                     /locus_tag="PKN91_03925"
     CDS             11746..12141
                     /gene="rpsF"
                     /locus_tag="PKN91_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNS27251.1"
                     /translation="MRHYEVVFLVHPDQSEQVPAMIERYKGMIAAGGGAVHRLEDWGR
                     RQLAFPIAKVHKAHYVLMNIETDQKVLAELTGAFRFSDAVLRHLVVAMDEAVTEPSPM
                     AKAEEDEAGGGRRERARDDADDDSVSDNA"
     gene            12166..12405
                     /gene="rpsR"
                     /locus_tag="PKN91_03930"
     CDS             12166..12405
                     /gene="rpsR"
                     /locus_tag="PKN91_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNS27252.1"
                     /translation="MSAPRQSRFSRRKKFCRFTAEGVKQIDYKDLATLKGYITETGKI
                     VPSRITGTKSFYQRQLSVAVKRARYLALLPYTDQH"
     gene            12414..12863
                     /gene="rplI"
                     /locus_tag="PKN91_03935"
     CDS             12414..12863
                     /gene="rplI"
                     /locus_tag="PKN91_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNS27253.1"
                     /translation="MDVILLQKVANLGTIGDRVSVKSGYGRNYLLPQGKATLATPANV
                     AKFEARRAELERVAREQHGDAELRAKAFADFTLTITAKAGSEGKLFGSIGTADIAEAA
                     TKAGQKLARSEVRLPEGPLRVVGDHVVNLHLHTDVNVPLAISIVAEE"
     gene            12905..14269
                     /gene="dnaB"
                     /locus_tag="PKN91_03940"
     CDS             12905..14269
                     /gene="dnaB"
                     /locus_tag="PKN91_03940"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNS27254.1"
                     /translation="MAGESRTPPHSIEAEQSVLGGLLLDSAAWDQVGDMLTAEDFYRP
                     DHRLIFESIGLLAGEGRPCDVITVSEQLERSGRLEDAGGLAYIGKLANETPTAANVRA
                     YAGIVRERALLRRLAKAGADISASVFQQQGESARDLVDRAEQSVFEIAEMGARGRDGA
                     LAVRSLLPALIDKIDEWHNNPDALRGLPTGFTDFDRKTGGLRGGDLMIVAGRPSMGKT
                     TLAVNIAEYAAVNPDIKASVVIFSMEMPSEQVITRMLSSIGGVPLNAIRSGKVADEDW
                     ARITSATSQLSEARIFVDETPGLSPTELRARARRGKREHGLDLIVVDYLQLMQVHGSK
                     ENRATEIAEISRGLKALAKELNVPVIALSQLNRGVEQRQEKKPVMSDLRESGALEQDA
                     DMILLIYREEVYDKNTTKKGMAEIELAKHRNGEIGTILLTFQGPYSRFTNYAPDSYAE
                     GVLR"
     gene            14266..15342
                     /gene="alr"
                     /locus_tag="PKN91_03945"
     CDS             14266..15342
                     /gene="alr"
                     /locus_tag="PKN91_03945"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNS27255.1"
                     /translation="MSRWIRAAIDTQALRANLARIRQVAAGRRVVAVVKANGYGHGAA
                     AVAHALADADAFAVARLEEGVGLRDAGITRPILLLEGVLSREQLEEAAARDFELVVHD
                     ASQLALLERWRGRRRFVLWLKINTGMNRLGFAPAEFAAAHARLAALAVPAAALRVMTH
                     LARADERDCDMTGEQLARFAALAAPLGLETSIANSAGIFGWLEAHGDWVRPGLSLYGV
                     SPFPGETASSLGLTPVMTLTSTVIAVREVPAGDCVGYGSIWRAPRAARIAILAAGYAD
                     GLLRSLPGGAPVLVGGRRAPLVGRVSMDMIAIDVTGLPGVEVGSPALLWGRDLPVEEI
                     ALAANTIPYELLCAVAQRVAFEVS"
     gene            complement(15315..16523)
                     /locus_tag="PKN91_03950"
     CDS             complement(15315..16523)
                     /locus_tag="PKN91_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNS27256.1"
                     /translation="MARSKAGPLLLCAAALTLAACGKEPPPQAATVTLPAELGELEGA
                     VDILVWPRHFARIEGDPRFDWITQFETDTGCRVTLTAVSTADEIVPLMAQGDHDLAVA
                     PGDATLRLIRGGLVQPINTLLVPSYNAVDIRLRRALWHYVDDVHYGVPFQWAPNWLMY
                     DTRVFDTPPGSWSAVLEPQAWPDGKANQGRVQAYGGAMQIADAALYLLYHQRDLGIMN
                     PYQLDDKQYTAALDLLRNQQPLLQGYWLDADEQVAAFGNGTVAASVSWPYQADRLAAD
                     RQPLAGVVPEEGATGWVDTTMLSARAKHPNCAYRWMEWSVNTRVQGDVAAAFGTVPVV
                     TAACEGNEKLGADGCSRHGADEFERIWFWRTPENTCDTALGRCVPYDRWVADYAALSG
                     TGQETSNATR"
     gene            complement(16510..17238)
                     /locus_tag="PKN91_03955"
     CDS             complement(16510..17238)
                     /locus_tag="PKN91_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNS27257.1"
                     /translation="MAAKTERLAVLIDAENAQASLIEELLAEIARYGTASVKRAYGDW
                     TTTRLSAWKEHLHRFAIQPIQQFGYTRGKNSSDSSLIIDAMDLLHAGNMDGYCLVSSD
                     SDFTPLATRLRESGAEVYGFGDKKTPEAFVAACDKFIFTEILRADAKQESAAPTASVP
                     PLEKLLRPAIDATARDNGWSPLSAVGSLLVKNHPSFDPRNYGFSKLSDLARKQRYVEV
                     NEVTSGDSATLYVRLKEDSRHGKK"
     gene            17270..18061
                     /locus_tag="PKN91_03960"
     CDS             17270..18061
                     /locus_tag="PKN91_03960"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNS27258.1"
                     /translation="MPASLEVPAKMNGDLSGRVALVTGASAGLGARFARVLAAAGATV
                     AIGARRTDRLAALAAELTQAGARAHAIALDVTRQDSIRAALEETERLAGPVDLLVNNS
                     GVSTETPILDTEEADWDRVLDTNLKGAFLVSQAVARRLRDTGRPGSIINIASILGLRQ
                     GSALTSYAVSKAGLVQLTKQMALELARYRIRVNAIAPGYIRTDMNREFFDSGRGDAML
                     KRIPQRRLGEPAELDAPLLLLAGESGAFMTGSVIVVDGGHLVSSL"
     gene            18058..19143
                     /locus_tag="PKN91_03965"
     CDS             18058..19143
                     /locus_tag="PKN91_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNS27259.1"
                     /translation="MSVDAGTDVTTAVRAGMEFDVGRLEIYLADAVEGFQGPLTVRQF
                     RGGQSNPTYLLDTPRRKYVLRRKPPGKLLASAHAVDREYRVIAALGAHTDVPVARAYA
                     LCTDDAVIGSWFYVMEHVVGRVYWDPTLPELPREARRGYYDAAVDALARLHRVDPAAV
                     GLADFGRAGGYLSRQIARWSKQYHEDEAAGSVPSMDRLIEWLPQNLPAAEPQPAIVHG
                     DYRLDNLMFAPDGPRVLAILDWELSTIGDPVADFAYYLMKYRMPGLALPGLAGRDLDA
                     LGIPSEGDSVAAYCARTGREGLPDLDFYMAFCMFRLAGIFHGIRGRVLRGTAVSARAR
                     EYAANVEPMADLAWAQASRGASPGRRH"
     gene            complement(19082..19606)
                     /locus_tag="PKN91_03970"
     CDS             complement(19082..19606)
                     /locus_tag="PKN91_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS27260.1"
                     /translation="MRVLETPRLVLRRLTTADAPFILELVNVPAFLRHIGDREVRNLD
                     DARAYIANGPVASYERHGFGLWHVALRTDGTAIGMCGLLKRDTLEHADIGFAYLPAWW
                     GQGYAREAAAAVLDYGTRTLGIPRIVAIVSPDNAGSIRVLEHIGLVRERSLPVNGDGR
                     ETLLYSPAPTRDPP"
     gene            complement(19616..20320)
                     /locus_tag="PKN91_03975"
     CDS             complement(19616..20320)
                     /locus_tag="PKN91_03975"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNS27261.1"
                     /translation="MARLILSLDSQVLAEYNMNKERYTIGRLPDNDIRIDNPAVSGHH
                     SLIINILNDSFLEDLNSTNGTYVNGKLIKKHALQHGDVVTVGHHQLRFVEDDEQQDEF
                     EKTMVIQPSARPVEKLRSVADGAAQAPSATGIRRALAEGQTAFKPAKLQVLSGAFAGR
                     ELELSKALTTLGRPGVQVAAITRRAEGYFIVHVDSGTPGDFPLVNGSAIGDQARRLQD
                     NDVIQLAGVKMGFFEG"
     gene            complement(20334..21182)
                     /locus_tag="PKN91_03980"
     CDS             complement(20334..21182)
                     /locus_tag="PKN91_03980"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNS27262.1"
                     /translation="MARRYGPPPSLRGKVRLVGLTDTGKVREHNEDTIAVDPDLGLLV
                     LADGMGGYNAGEVASGIAVKTIVNLVREAMEREDMTAIDPTTKLTRPSIILRDAITRA
                     NKIIYQTARSQPQCEGMGTTVVSALLFDNRISIAHVGDSRLYRLRGPKFEQITMDHSL
                     LQELVDRGFYSPEEAQRAANKNYVTRALGVEANVEVELQEVPVQKGDHYVLCSDGLND
                     MVEDSDIHLTVNTFSDNLDAVAKHLIQLANDNGGRDNVSVVMAQILESFPARRGVFDR
                     VLGWFS"
     gene            21440..22807
                     /gene="radA"
                     /locus_tag="PKN91_03985"
     CDS             21440..22807
                     /gene="radA"
                     /locus_tag="PKN91_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNS27263.1"
                     /translation="MARPVLVHVCSACGAESPKWQGQCPQCGEWNSLQQQAAPRAPAA
                     ARPGRGTVRAAPARLAPAGAGPARFPSGLAELDRVLGGGIVPGSVTLIGGDPGIGKST
                     LLLQVAASATASRAMLYATGEESLEQVAMRARRLGLASDALSVLAETDLGTILEAARN
                     LGVALLVVDSIQTVQFAELGASAGAVTQLKECTAQLVRFAKTSGIAVMIIGHVTKEGA
                     IAGPRVLEHLVDAVLYFESEAGSRFRIVRAVKNRFGAVNELAFFLMAEEGLREVRNPS
                     AIFLARAAEAAPGSIVMVARDRGRPLLIELQALVDRMRFGAPRRVAQGLDANRLAMLL
                     AVLNRHADVSLQEHDVFANVVGGLDLGETATDLPLVLALTSSLRDRPLPQSLVAFGEV
                     GLTGEVRPVAYGEERLREAAKQGFRVAVVPQGNVPRKPIEGLEVRGVGRVPEALTAAF
                     ARAPS"
     gene            complement(22776..24041)
                     /locus_tag="PKN91_03990"
     CDS             complement(22776..24041)
                     /locus_tag="PKN91_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HNS27264.1"
                     /translation="MDTAPTTVLVIALVALLATIAFSSGTEVAMLAVNRYRVRHRAAG
                     GHRTARVLDSLLARPDDWLGANLVILALASVAASSIATLLAQRYGGEWAVPVTIIALS
                     IVMIVFCELAPKIYAARHPEPVALGSTYIYRALVWVAKPLLWITNQLAYGFLRLFGVR
                     RANRGGQTLSADELRTVVAETGTVIPQGHRRMLLSILDLGQITVNDIMIPRQDIAGID
                     LDDDWDDILAQIRQTPHTRLPVYRGTLDDVVGLLHMKRVAQELARGTLTRGRLEELAR
                     GREPYYVPEGTPLTVQLTHFQRNRRRMGFVVDEYGDIEGLVTLEDLLEEIVGEFTNDT
                     STVVHKDVTAESPGVFLVNAAATIRALNRALGWTLPTDGPKTLNGLLLERFETIPDAG
                     AALRLDGLQFEVLQVQDNAIRTVRVRTPQ"
     gene            24122..25474
                     /gene="ffh"
                     /locus_tag="PKN91_03995"
     CDS             24122..25474
                     /gene="ffh"
                     /locus_tag="PKN91_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNS27265.1"
                     /translation="MFDNLTARLSRTADALRGRGRITEENIAETLRDVRMALLEADVA
                     LPVVKRFIENVRAKALGAEVQQSLTPGQVFVGILHKELVALMGGAAAGFSLRAQPPFV
                     VLLAGLQGAGKTTTAAKLARWLGETHKKRVLLVSTDVRRPAAILQLERLAAQVGAGFY
                     KADPATPPARIAAQALEQAKRGLYDVLLVDTAGRLHVDQELMQEAREIDLAVQSHERL
                     FVVDAMAGQDAVNAARAFGEALDLTGVILTKADGDARGGVALSVREVTGRPIVFVGVG
                     EKTEALEPFDPERMANRILGMGDVVALVEQVQKQVDVDEAQRLARKVAKGKAFDFNDL
                     RGQLEQLLKMGGLAAVMDKLPQHMTRAAAANAPGDRDVRRQIAIINSMTRRERRQPAV
                     IDGSRRRRIAAGAGVQVQDVNRLLKQFQEMQRMMKSMKGGRLARMLGAMKGGRPPGFL
                     "
     gene            25545..25799
                     /gene="rpsP"
                     /locus_tag="PKN91_04000"
     CDS             25545..25799
                     /gene="rpsP"
                     /locus_tag="PKN91_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004809941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNS27266.1"
                     /translation="MVTIRLTRRGAKNAPFYHVVVTDSRKRQGGKSLEQVGFFNPIAR
                     KGDTKLKLDLPRIDHWVSKGAKPSVRVATLVSDYRKQAAA"
     gene            25850..>26124
                     /locus_tag="PKN91_04005"
     CDS             25850..>26124
                     /locus_tag="PKN91_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27267.1"
                     /translation="MRGFSHVDSFTDPPELLSEFRSWRLRNAQGAVAEYEVAEMRAHG
                     ARFVARLVGVDSREGAARLTGAVIEVERDALPDPGDRQHYWVDLIGLA"
BASE COUNT         4140 a         8695 c         8996 g         4293 t
ORIGIN      
        1 gcgcgatcgg ggtggatcgc atggcacagt tcgaggtgtg cgcccgcggc tttcgcaagc
       61 tgcgcagcct tgcgcaactg accggggggg acatgccagg gatcgccgat cgccaccagg
      121 attcgcttga tcttgagtgt catgtcgcaa gtgtacacgc gccgccggcg tgtcagagta
      181 ccaccgtctc gtggtcgctc gggatccgca cgtcgcaatg gaaacgcgac tcgattccgg
      241 ctgccagcgc gcgtgacgcg tgctcttcgc catggacgag ccaggtcact gccggtggcc
      301 gcttgaattt cgccagccag tcgagcaggg ccgtgcggtc ggcgtgcgca gagaatccgc
      361 cgagcgtggc gacctcggca cgcacgggga cctcctcgcc tgatatgcgc acgacgtccg
      421 cgccatcgac gatgcggcgc ccgagcgtgc cggcggcctg gtagccgatg aaaatgacgg
      481 tagtgcgcgg gaagggcagg tgctggcgca ggtggtgccg gatgcgcccg ccatcgcaca
      541 tgccgctcgc ggcgacgatg attgccccgc cggtcagcct gttcagagat ttggactcgt
      601 cgaccgtctc gatgaagcgc acgcgcggcg cgactccgtc cccgggcggc gcctgtgcgg
      661 cccggcgcgc ttcggcatcg aacaggtcga aatggcgcgc ggtgatgtgc gtgacctcgg
      721 ttgccatcgg cgagtcgacg aatacgtcga ggtcgcgcag ccgaccggcg cgcgcgagcc
      781 cgttcaggat gtagaggaac tcctgggtgc gtccgaccgc gaaggccggt acgagtacca
      841 tgcccttctt cttgtacagg gcccgctcga gcgtctcgac cagttcgtcg atcgtggccg
      901 tgtacggccg gtgatcgcgg tcgccgtagg tcgattcgag cagcagcacg tcggcatcgg
      961 caatggctgt cggatcgcgc acgatgggtc tgcccggttg cccgaggtcg cccgagacga
     1021 cgaccttgcg ctggctcacg ccatcgtcga gccacagctc gacgatggcc gcaccgatga
     1081 tgtggccggc gtcgcgcagg cgcacgcgga cgcccgtgcc gggctcgaag atgtcctcgt
     1141 agtcgacggt gcggatgagc ggcagcaccg catccacgtc ggccatcgag taggcggcga
     1201 catagtcgcc accgcggcgg cgcgagcgct cctcctcgac ctgctgcagg taggcgctgt
     1261 cgcgcagcag gacggccgtg atgtcgcgcg tggccgcggt cgcgtaaatg gggcccgaga
     1321 agccctcggc ggcgagtctc ggcagcaggc cgctgtgatc gagatgggcg tgcgtcagca
     1381 cgacgaagtc gagctccgcc ggctcgaagg cgaacggcgc ggtgttcgcc tcccgcgtcg
     1441 cgcgcgcgcc ctggaagagc ccgcaatcga ccaggaagcg gctgtggccg acctcgaagg
     1501 cgaagcacga gcccgtcacc tggcgggccg ccccgtggaa ggtcagtcgc atgtcgacat
     1561 cctcaggcga ttggtttgag taccccgaag aatgccacgg ttgcgatgcc gacccagtag
     1621 aaataggcga ggatccgcga cagggcgatt tccttcgcca gctgtgcctc cacggcggag
     1681 cgcgaggcgc cgctttcgag gctgcggaac agcgtgaccc agtggcgcat gacgacgcgc
     1741 agcacgagcc cgatgatcag caggatggcg tacagcgtga ccttgaacgc ataccagcgc
     1801 gcgccgagcg ggcccgcgcc gaacaggccc atcacgccgg ggatgagcag cgcggggatc
     1861 agcacgtagc gcagcgcctc gtcgatgcgc gtcaggcgca gtccgaggtc gctctggtgc
     1921 gtgtagtagg cggagagcgt caggcacaac caggcaccga agaagatcca tgtgccgacg
     1981 atccacggac cgccgagcgg ctgcaccccg aggttgtaac ccatgtgcag gccgagcggc
     2041 agcagcagga tgatgccgag gcgcgcgagg atgtcgatgc gaaacgccgt atccatgtgc
     2101 cggcgccgct cgtcgagcga caggttgacc ttggtgacat agcgcgcggt attgaacacg
     2161 ccccattccc cgcccagcca gtagaccatg gcgagcacgt gcagccagcg cagtaccccg
     2221 tgtgtcgtga tgtagaagtc cattagccgc tcccgccgga tggatgatcg ggcaaatcat
     2281 gggcggctgg tttgcgcccg gacaagtgta aatacccgta ttcgccgtcc gttcgtcaag
     2341 ccgcatctgg ctgtgagtct ggggctcaaa gcgatcgagg ccggataccg cgtggtgttc
     2401 acgaccgccg cgcacctgat cgcggtactg gccaagacgc acgcggaagg ccggctcgat
     2461 gagaagctga aggtctttac cacgccgcgt ctgctgatca ttgacgagat cggctatctg
     2521 ccgatcgatc ggctgggggc gaacctgttc ttccaactca tcagtcggcg ctacgagcgt
     2581 gggcccatga tcctcaccag caaccagagc ttcggcgcct ggggcgaggt attcggcgag
     2641 cgcactatcg ccaccgcaat cctcgatcgt ctgttgcatc acgcggtgac gctgaacatc
     2701 cgcggcaact ccagccggat attctcagcc tcaagctcct tcagcctgcg tagctgcgag
     2761 atatccaggc cactgcacct ggccttcacg ccggcttcat gtgccctagc ggccgggggt
     2821 gatgccacac gatcgacctg ccgccggcgt tccgcttgcg tcaccacacc gtgagcgtga
     2881 caatgtggac caaattggtc ccctatctca tccacacccg cgaaagagca gtccaatgcc
     2941 agaagccgca tccccatcca ccgcccacat cttcgatgcg cgcggtgtcg cacgccgttt
     3001 ccgccactca gccatcttcg gcgcactggg cgcgctgcag ccgggcgagg cgatgcggtt
     3061 cgtcaacgat cacgatccac tgccgctgct cgatcagctg cggcagcgct tcggcgagca
     3121 catcgcaatc gagtatcagc agcgcgagcc gggcgcgatc gtcatcgatt tcacgatcgt
     3181 cgcctgagcg gggtccaagg cacagccgcg cagtcatgtg cgcgctacgg cggccttgcg
     3241 cgagccgtgc gcggcgtgcc cggtttcggc gggcgcgcgt tcggttcagg cgaggcgctc
     3301 gaaacgcgcc tggaagaacc tgaggtagcg cggttcgtgc accatgcgca gcccgaccgc
     3361 gctgtcccgc gcgtcgaaca cggccgccgc cgattccgcc acgatgtcca tgtgggcctg
     3421 cgtgtagaca cggcgcggaa tcgtcaggcg cacgagctcg agcgcggggt ggtggtgttc
     3481 gccggtttcc ttcgagcggc cggcggacac gatgccacgc tccatggcgc gtacgccggc
     3541 atcttcgtac agatatgctg ccagggcctg ggccgggaac tgctcctggg gcaggtgcgg
     3601 gtagaaggcc ttcgcatcga gatacaccgc gtggccgccg atcggccgca cgaccggcac
     3661 gcccatgtcg atcagcatgc ggccgaggta ttcgacctgc ccgacgcgcg ccttgatgtg
     3721 ctcgtcgtcg accgattcca cgatgccgcg cgccatcgcc tccatgtcgc gtccggccat
     3781 gcccccatag gtatgcagtc cctcgtagag cacgacgaga ttgctcgcgt cctcgtacag
     3841 gccggtatcg ttgacggcga gccagccacc gatgttgacg agcgcgtcct tcttgccgct
     3901 catcgtgcag gcgtccgaat atgagcacag ctcgtgcagg atgtcggcga ccttgcggtc
     3961 gcgatagccg tcttcgcgct gctggatgaa gtacgcgttc tcgaccgcgc gcgtcgcatc
     4021 gagcacgatc cggatgccat gccgttgcgt gagttcgcgc acggcgcgca gattcgccat
     4081 cgagacgggc tgtccgcccg ccatgttgac ggtcacggcg acgcacacat agggaatgcg
     4141 ctgcgcgccg actttcgcga tcaatgcctc gagcgcgccg aggtcgacgt tgcccttgaa
     4201 cgcgtgctca ctcgccgggt cgtgcgcctc gggaatcacg acgtcgacga aagtgccacc
     4261 ggcgagttcc tggtgcgcgc gggtcgtcgt gaaatacatg ttgcccggca cgtagtcgcc
     4321 cggctcgacg aggatcgtcg agagcagatg ctccgcgccc cggccctggt gcgtcggcac
     4381 gacgtgccgg tagccgtaat acttccgcac cgtttcctcc aggtggtaga agttgcggct
     4441 gccggcgtac gcctcgtcgc cgagcatcat gcccgcccac tgccagtcgc tcatcgcgct
     4501 cgtgccgctg tcggtgagga ggtcgatgta gacgtcttcg gacctcagca ggaacgtgtt
     4561 gtagcccgct tcggcgagcg cacgcgcacg ctccgcgggt gtcgtcatcc tgatcggctc
     4621 gacgaccttc accttccagg gctctgccca cgagcggcgg cccggacgtg ggcggggacg
     4681 ttcggcgggc gtcgtcatgg gtcggctcca cggaagtgac ggggcaggtt aggctgccga
     4741 caaattcaaa gaaagaatat gatattggag gactgcgaca aagaattgga acatcatgcg
     4801 cctcgatctc gatgccatgg aagcgctcga agccgtcgtg cgttgcggcg ggcttgcccg
     4861 cgccgccgag gagctgcaca aggtgccgtc ggccgtgagt taccagctgc gcaggctcga
     4921 gcagcaattg cgtgtggctc tggtggatcg cgagggctat cgggtccgct tgacgccggt
     4981 cggcgaggtg gtgctcgcgc agggccggcg cctgcttgca caggcgcgcc aggtcgagtc
     5041 gctcgcccgc caattcgccg acgcctggga accgcgcctg ctcgtgatcg tcgatggcat
     5101 cctgccggtc aagccgacct tgaccgcatt caatgcattg tccagtgagg gcgcgcccac
     5161 ccgttttcag gtgaaggtgg agttccagcg tggtgtgcag ttctgctttg aacgcgacga
     5221 cgccgatctg atggtggtca aggactacga tccgaaaccc tatctcgtcg cgaaagagct
     5281 ggcggcggtc gagtgcattc tctgtgtcgg gcggcgccat ccgctcgctg cgcaacgttc
     5341 cgtgagcctg gaggacctgc aggattacgt ggagctttcc gtccaggatt cgagcggcaa
     5401 gcgtgacgat ccacatggat tcggcggaga gcgggtcttt catctctccg gtttcgatgc
     5461 caagaagcag gcactgctga tgggcgcggg ctttggctgg atgccgcacg ggatggtgcg
     5521 cgccgagttg cgcgcgggac gcctgcgcga actgcgctat gccggaggct cgcgctatcg
     5581 gttcacgccg cgcctcgtgc atcgcctcga ccggccgccg gggcgggcag ggcggcggct
     5641 cgcgcaattg ctgcgcgagg cttccggcaa ggatggcgat ctgatcggcc acgactgatc
     5701 gagcaaccgg aaacgtccgt gctgcatcag gtttcgatgc aagcccggaa aatcgcacgc
     5761 aaggcaaggt tcttgcgcag tctcgggacc ttgtgaggca tcccgaaacc ccgctctggt
     5821 acgtgcagcc ggcgcagtat ccggtgcgtc gcgatgtaga attcccttgc cccgcctgac
     5881 ggacgatcgg gccaatgatg tgtgcccgat ttggccccgg actggtgtca atatccctta
     5941 tccgccgtgt gtcggccgcc ggggcaggcg ggtccgcggg tccgcgggtc ctcggcacac
     6001 ggcttgcccg catgattgcg agcctgcgca ggcttgcatc accgcgacca gcgaagtcag
     6061 tggagcagac gcgcatggga gagcaggcct ggtgggctac cgggattgcc gatctggcga
     6121 agcggctggg cagcggcccg gaaggcctcg gttccgctga agcgggaact cgtgcccagg
     6181 gccgccctcg acgatcgcgc ggcggccagg ccttgcgcct gtttctttcc cagttcacca
     6241 acccgctggt cctgatcctg atcgccggtg ccgtgctctc gctgttcctg cgtgaatggg
     6301 tggacgcagc aatcatcctg gtgatcgtca tcggcagcgg cctgcttggc ttcacccagg
     6361 aacatgccgc ctccagagcc atcgaggacc tgcggcaacg cctggtcagg cgcgtgagcc
     6421 tgcttcgcga tggcaaggag gcgaggatcc cgctcgacga agtcgtgccc ggcgacgtcg
     6481 cactgctgca ggcgggcgac ctggtccccg gcgatggcag gatactggac agcgcggctt
     6541 tgctggtcga tgaagctgcc ctgacgggag agcccttccc cgtcgaaaag tcgccttcga
     6601 tcgcccccga atccgcaacg ctgatgcagc gtaccggcgc gttgttcaag gggacctcga
     6661 tccgcagcgg aagctgtcgc ctgctggtgg taacgaccgg caaggatacc gtcttcgggt
     6721 caatcgagcg ggacctggag agggccgagg aaaccaccga gttcgcgcgc ggcctgactg
     6781 gcttcggcaa catgctgatg cgcgtgatgc tggtcgtcgt cgccatggtg ctggtcgcgg
     6841 cgactctgct gggacgtgac ctggtgacat ccatcctgtt cgccatggct ctcgccgtcg
     6901 caatctcgcc tgaactgttg cctgcgatcg tcagcgtgac cctcgccgcg ggagccaggc
     6961 gaatgacccg gcacggcgtg ctggtcaggc ggctccaggc aatcgaaaac ctgggaggaa
     7021 tggacatcct gtgcaccgac aagacgggca cgatgaccgt cggctcgatt gcgctcagcg
     7081 gagcggtgga tacctccggc caggcctcag gcgacgtgct gcgcctggcg gcgatcaatg
     7141 cacggctgca gacgagcatg gcgagcgcgc tggaccaggc gatcctggct gcggcctcgg
     7201 ccgccagcct ggccagcgaa atctccggga aactgagcga gattccctac gatttcgtgc
     7261 ggcggcgttt cagcgtggcg gcacgcctca ctgatggcac ggaggtcttc gtcaccaagg
     7321 gagcggtcaa cgaagccctc tcaacctgca cgcgggcacg ctacggcgac ggtgtccatg
     7381 cactcgacgc gggagggcgg gatacccttc tgtccttcgt caggtcgaag gccgaaagcg
     7441 gattccgcgc gctggcagtg gcaaccggtg acgaaggcga agcctgcctc gaaggtttcc
     7501 tgctgttcct cgatccgccc aagccgggca tccgggacac ggtcgcggcc atggcgagtc
     7561 gcggcatttc ggtccgcata gtcagcggag acaaccgcca cgtgacggca aggatggccg
     7621 gggtcatcgg tctggaaccc ggcgaggtcc tgaccggcga tgagatcgcc gcgctggacg
     7681 atgacgctct tgccgcgcgc atcggtggcg tgcgcgtctt tgccgaagtg gaaccgcagc
     7741 agaaggagcg tgtcgttcgc gccttccgca aggccggcca tgcggtcggc tacatgggtg
     7801 acggcatcaa tgatgccccg gcgctgcgcg cggccgacgt cggcatttca gttgacagtg
     7861 ccgtggatgt ggcccgcgag agcgccgaca tcgtgatcct gcgccccgac ctgggggcca
     7921 tccagtgcgg cgtggaggaa gggcggcgta cgttcgccaa tacgctcaag tatgtctcga
     7981 tcacgatcag cgcgaatttc ggcaacatgg tgagcatggc gttcgccacg gtgttcctgc
     8041 ctttcctgcc gctgttgccg acacagatcc tgctcaacaa tttcctgtcc gacctgcccg
     8101 cgatcgcgat cgccggcgat gccgtggatc gcgagcggat ggcctcggcc cagcgctggg
     8161 acatccggcg catccgcaat ttcatgatcg tgttcggcct catcagcacg gtcttcgacc
     8221 tgctgacctt cgccttcctc tggctggtca tgcgggctga cccggacacc ttccggacca
     8281 gctggttcat tgtctcgctg ctgactgaac tggctgcgct gctggtgctg aggaccagac
     8341 ggcgcttctg gcgcagcccg ccctcacgac tgctcgtgtg gagttcggtt cttgtcgccg
     8401 tactttccct ggcgctgccg tacagcggcg aaatcgcagg cctgtttgca ttccgccccc
     8461 tggcccctgc gctggtcggc ctgctgctcc tgattttcgc cggctacgcg ctcgtgaccg
     8521 agttcgccaa acgccggtat ggcatccagg cgcattagag gtgaccgtcc tgcggcgtgg
     8581 gtctcgcgga ctgccaatgg tcgcgtggtg ggggagaacc ggggcgatcc ggcaagatcc
     8641 acgggccgat tgccgcggat tcccacgggt ccaatatact tcttgcggtg ctccgggaag
     8701 caaatggtgc ccaggagagg actcgaacct ccacggtgtt acccgctagc acctgaagct
     8761 agtgcgtcta ccaattccgc cacctgggca ggtgggccgc gaaatctacg catgcccccc
     8821 ggctctgtca attaggctac gcgcatgaag agaacccgca aaccatccca gaaggatcgc
     8881 ggcgcgcgcg gcgcgcatcc gacccaacgt cgccaggggc agcgtgcagc cggccaggcc
     8941 gggcacaagc gcgccgccgt caaggccgcc accggaccca tggccaaggg catcgtcgaa
     9001 ggcgtcgtca gtgcgcatcg cgccggctac ggctttgcgc gcatcgaagg catgaccgat
     9061 tccgccttca ttcccccctt tgcgatggcc ggcctgatgc acggggatcg catccggctc
     9121 accgtcaagc gcgatgccac gggccgctac accggcgagg tgctcggcat cgtcgagcac
     9181 ggcgtgaagg cgttcctcgg cacgatcgac gtcgaggggc gcgcggcgta cgtgctgtcc
     9241 gccgaccggc gcctcgcgct gcgttgccag gttgcggcgg ccgacctgaa cggcgcgaag
     9301 cacggcgact gggtcatcgc ggtggtcact cgctacccgg gggcgaaggg cggggcgcat
     9361 gcgcgaatcg agcgccgcct cgatcccgat cggccggtcg agctcgcgac cgagacggcg
     9421 attgcgcgct tcggtctgcc gaccgacttc cccgccgagg tgcgggccga ggcggaggct
     9481 tgcgggcagc acgtcgatcc gcgggaagcc gcgcagcgtg tcgacctgcg tgcgctgccg
     9541 ctcgtcacga tcgacggcga ggatgcgcgc gatttcgacg acgcggtcta cgccgagccc
     9601 cacgaacacg gattccgcgt ggtcgtcgcg atcgccgatg tgagccacta cgtacgccag
     9661 ggcagcgcgc tagacatcga ggcggcgaag cgaggcacgt cggtgtattt ccccctgcgc
     9721 gtcattccga tgctgccggc ggcactgtcc gatcacctgt gctcgctgca gccgaaggtc
     9781 gaccgcctgt gctttgcggc cgacatggtg atcacgaagc aggggcagct gaagtacgcg
     9841 cgcttctacc cggctgtcat gcactcggcc gcgcgcctga cctacacgct tgcctacgag
     9901 gcgctgttcg agggccgacc cgaggcacgc aaggcgatcg gtccgctgat cgagcggctc
     9961 ctgccgctgg tcgatgtcta ccgcgcgctg cacaaggcgc gcaaccgtcg cggtgcgctc
    10021 gatttcgatg cgcccgaggc gaccttcgac atcgactcgc aggaccgggt gaagggcatc
    10081 gtgctcgcct cgcgcaacga cgggcatcgc ctgatcgagg aatgcatgat cctcgcgaac
    10141 gtgaccaccg cgcacgagct ggcgcgccag cgtgtcggtt cgctctaccg cgtacacggc
    10201 aagccggacg aggaaaagct cgaccggctg ctctcgacgc tcgccgcgct cgggttcccg
    10261 gccgagattc ccgagacggt cacgccgcgc gacatccggc agatcacgca ccggctggga
    10321 gcctcgcccg cgcgaccctt cgtcgagtcg ctggtcgtgc gctcgatgcc gcaggccgtc
    10381 taccagagtg cgaacattgg ccacttcggc ctcgcgctca cgggctatgc gcatttcacc
    10441 tcgccgatcc gccgctatcc cgatctcgtc gtgcaccgga gcctgaaggc gatgctcggc
    10501 gtgccggcgc cgatcggtgt gcgctacggc gcggaggagc tcgcgagtct cggcgagtcg
    10561 acctcgcggc tcgagaagcg cgccgacgag gccgatcgtt atgtcgacca gttcctgaag
    10621 tgcagctggc tgcgcgagcg gctcggccag actttcgagg gtctggtcac ggcggtggtc
    10681 gagttcggct gcttcgtgca gatcctcgag atttcggcgg atggcctgct gcacaccgcg
    10741 acgctcgtcg acgatgccta cgaactcgat gccggcggcc atgcctgggt cggcaagcgc
    10801 agcaagcgcc gcctgggcct cggcgagcgc atccgcgtga tcgtgacgaa cgcgaatccg
    10861 gtggaaggcc tcatcgacct ggagctcgcg tgagcgcgac cgagcgcctg tacggcctgc
    10921 acgcggtgcg cgccgtactg acgcgcgatc cgtcgcgggt cacgcagctg acactcaccc
    10981 accagcgcga cgacaagcgc attgccgccc tggaggcgct cgcccgggat gcaggtgttg
    11041 ccgtgcgccg ggtcgacgcg cacgcgctcg cggggctcgt cggcgacgtc gtgcaccagg
    11101 gagtggtcgc cgacgtctcg ccgctcaagt cctggtccga ggacgacctg atcgatgcgc
    11161 tgcgcgaggt gaaggcgccg ctcgtgctgg cgctggacgg cgttcaggat ccgcacaacc
    11221 tcggggcctg cctgcgcacg gccgacgcct gcggggcact ggcggtggtg gtgccgaagg
    11281 accgcgcggc gcaggtcaat gcgaccgtca ggaaggtcgc cgccggtgcg gcggagacta
    11341 caccggtcgt ggccgtcacc aatctcgccc gcacgctcag gctgctgaag gcggcgggcc
    11401 tgtggatcgt cggcgccgca ggcgaggcgg acaaggtcgc cgccggggtc gatctgaccg
    11461 ggccgctggc cgtcgtgatg ggcgcagaag gcagcggatt gcggcaactt acccgccaga
    11521 actgcgattt tctcgtacgt ttgcctatgc tgggggcggt cgagagcctg aacgtctcgg
    11581 tggcgaccgg catgctgctc tacgaggtca tacgccagcg cacacacgcg ggggcctgag
    11641 cccccggccc gcattgcgac cccggggccc atcgggtatc ctcgcgcgcc ttttccttgc
    11701 cacaccgccg cgcctcggcg ggtggctcac ccacaaggag cacacatgag gcattacgaa
    11761 gttgtcttcc tggtccatcc cgaccagagc gaacaggttc cggccatgat cgagcgctac
    11821 aagggcatga tcgcggccgg cggcggtgcg gtgcatcgcc tcgaggactg gggtcgacgc
    11881 cagctcgcct tccccatcgc caaggtccac aaggcgcact acgtgctgat gaacatcgag
    11941 accgaccaga aggtcctcgc tgagctcacc ggtgcgttcc gcttcagcga tgcggtgctg
    12001 cgccacctcg tcgtcgcgat ggacgaggcc gtgaccgagc cctcgccgat ggcgaaggcc
    12061 gaggaagacg aggccggcgg cggacgccgc gagcgtgcgc gcgacgatgc cgacgacgac
    12121 agcgtcagcg acaacgcctg agcctgagca cccgaggaat acagcatgtc agcaccccgt
    12181 cagagccggt tctcccggcg caagaagttc tgccgcttca ccgcggaagg cgtcaagcag
    12241 atcgactaca aggatctcgc gacgctcaag ggctatatca ccgaaaccgg caaaatcgtg
    12301 ccgagccgta tcaccggcac gaagtcgttc taccagcgcc agctctccgt ggccgtcaag
    12361 cgtgcgcgct acctcgcgct gctgccgtac acggatcagc actgaggttc gccatggacg
    12421 tcattctcct gcagaaagtg gccaacctcg gcaccatcgg tgaccgcgtc agcgtcaaga
    12481 gcggctatgg ccgcaactac ctgctgccgc agggcaaggc gacgctcgcg acgcctgcca
    12541 acgtggcgaa gttcgaggcg cgccgcgccg agctcgagcg cgtcgcgcgc gagcagcatg
    12601 gcgacgccga gctgcgcgcc aaggcattcg ccgacttcac gctgacgatc accgcgaagg
    12661 cgggttcgga aggcaagctc ttcggctcga tcggcacggc ggacatcgcc gaagccgcca
    12721 ccaaggccgg ccagaagctc gcccgcagcg aagttcgcct gccggagggg ccgctgcggg
    12781 tcgtgggcga tcacgtcgtg aacctgcacc tgcacaccga tgtgaacgtg ccgctcgcga
    12841 tttcgatcgt cgccgaggag taattccgct gtctttccgc ccgagtccac tgcctactag
    12901 tgcgatggcg ggcgagtcgc ggaccccacc gcattccatc gaggcggagc aatccgtcct
    12961 cggtgggctg ttgctcgact ccgccgcctg ggaccaggtc ggcgacatgc tgacggccga
    13021 ggacttctac cggcccgatc accgcctgat tttcgaaagc atcggcctgc tggccggcga
    13081 gggccggccc tgcgacgtga tcacggtgtc cgagcagctc gagcgcagtg ggcggctcga
    13141 agacgcgggc ggtctggcgt acatcggcaa gctggcgaac gaaacgccga ccgcggccaa
    13201 tgtgcgcgcc tatgccggca tcgtgcgcga gcgcgcgctg ctgcgccgcc tcgccaaggc
    13261 cggcgccgac atcagcgcat cggtcttcca gcagcagggc gagagcgcgc gcgacctggt
    13321 ggatcgcgcc gagcagtcgg tattcgagat cgccgagatg ggggcgcgcg gccgcgatgg
    13381 cgcgctcgcc gtacgctcgc tgctgcccgc cctgatcgac aagatcgacg agtggcacaa
    13441 caaccccgac gcgctgcgcg gcctgccgac cggtttcacc gacttcgacc gcaagaccgg
    13501 cggcctgcgc ggcggcgacc tgatgatcgt cgcggggcgt ccgtcgatgg gcaagaccac
    13561 gctcgccgtg aacatcgccg agtacgcggc ggtcaatccg gatatcaagg cctcggtggt
    13621 gatcttctcg atggaaatgc cctccgagca ggtcatcacg cgcatgctgt cgtcgatcgg
    13681 tggcgtgccg ctgaatgcca tccgcagcgg caaggtcgcc gacgaggact gggcgcgaat
    13741 cacgagcgcg accagccagc tgtcggaagc gcggattttc gtcgacgaga cgccgggtct
    13801 gtcgccgacc gaactgcgcg cccgcgcgcg ccgcggcaag cgcgagcacg gcctcgatct
    13861 gatcgtggtc gattacctgc agctgatgca ggtgcacggt tcgaaggaga atcgcgccac
    13921 cgagatcgcc gagatctccc gcggcctgaa agcgctcgcc aaggaactca atgtgccggt
    13981 gatcgcgctg tcgcagctga atcgcggcgt cgagcagcgc caggagaaaa agccggtcat
    14041 gtccgacctg cgcgagtcag gcgcgctcga gcaggacgcc gacatgatcc tgctgatcta
    14101 ccgcgaagag gtctacgaca agaacaccac caagaagggc atggccgaaa tcgaactcgc
    14161 caagcatcgc aatggcgaga tcggcaccat cctgctgacc ttccaggggc cgtacagccg
    14221 cttcaccaat tacgcgcccg acagctatgc ggagggcgtg ctgcggtgag ccggtggatc
    14281 cgcgccgcca tcgacacaca agccctgcgc gccaatctcg cccggatacg ccaggtcgcg
    14341 gcgggccgcc gtgtcgtcgc ggtcgtgaag gcgaatggct acggccacgg ggccgccgcg
    14401 gtggcgcatg cactggcgga cgccgacgcc ttcgcggtcg cgcgactgga agagggcgtc
    14461 gggctgcgcg atgccggcat tacccgaccg atcctgctgc tcgagggtgt gctgtcgcgc
    14521 gagcagctcg aggaagccgc ggcgcgcgac ttcgagctcg tcgtccacga tgcctcgcag
    14581 ctcgcgctgc tcgagcgctg gcgcggcagg cgccgattcg tgctctggct caagatcaac
    14641 accggcatga accgcctggg tttcgctccc gcggaattcg cggccgcgca cgcgcgcctc
    14701 gctgcgctcg cggtaccggc ggcggcgctg cgcgtgatga cccatctcgc gcgtgccgac
    14761 gagcgcgact gcgacatgac gggcgagcag ctcgcgcgct ttgccgcgct cgccgcgccg
    14821 ctcggtctcg agaccagcat cgccaactcc gccggcatct tcggctggct cgaggcacac
    14881 ggcgattggg tgcggcccgg gctgtcgctg tacggcgtgt caccgtttcc gggcgagacg
    14941 gcatccagcc tgggcctcac gcccgtgatg acgctgacct ccaccgtgat tgcggtgcgt
    15001 gaggtgccgg ccggcgattg cgtgggttat ggcagcatct ggcgcgcgcc gcgcgcggcg
    15061 cgcatcgcga tcctcgcggc gggctacgcc gatggcctgc tgcgcagcct gccaggcggg
    15121 gcgccggtgc tggtcggcgg gcggcgcgca ccgctcgtcg ggcgcgtgtc gatggacatg
    15181 atcgccatcg acgtcaccgg cctgccgggc gtcgaggtgg gcagtcccgc gctgctgtgg
    15241 ggacgcgact tgccagtcga ggagatcgcg ctggccgcga acacgatccc ttacgaactg
    15301 ctgtgcgccg tggctcagcg cgtggcgttc gaagtctcct gaccggtccc ggacagcgcg
    15361 gcatagtcgg cgacccagcg atcgtagggc acgcaacgac cgagcgccgt gtcgcaggtg
    15421 ttctccggcg tgcgccagaa ccagatccgc tcgaactcgt cggcgccgtg cctgctgcag
    15481 ccatccgccc cgagcttttc gttgccttcg caagccgcgg tcacgaccgg cacggtgccg
    15541 aatgccgcgg cgacgtcgcc ctgcacgcgg gtgttcaccg accactccat ccagcgatag
    15601 gcgcagttcg gatgcttcgc gcgggccgac agcatcgtcg tgtcgaccca gccggtggcg
    15661 ccttcctccg gcaccacgcc ggcgagcggt tgcctgtccg ccgcgagccg gtcggcctgg
    15721 tacggccacg agaccgacgc agccacggtg ccgttgccga acgcggcgac ctgctcgtcc
    15781 gcatcgagcc agtagccctg cagcagcggc tgctggttgc gcagcaggtc gagcgccgcc
    15841 gtgtactgtt tgtcgtcgag ctggtacgga ttcatgatgc cgaggtcgcg ctggtggtag
    15901 agcagataca gcgcggcgtc ggcgatctgc atcgcgccgc cataggcctg tacccgaccc
    15961 tggttggcct tgccgtcggg ccacgcctgc ggctcgagga cggccgacca cgagcccggc
    16021 ggcgtatcga agacccgcgt gtcatacatc agccagttgg gtgcccactg gaacggcacg
    16081 ccgtagtgca cgtcgtcgac gtagtgccac aacgcgcgcc gcaagcggat atcgacggcg
    16141 ttgtagctcg gaaccagcag ggtgttgatc ggctgcacga gaccgccgcg gatgagccgc
    16201 agcgtcgcat cgccgggcgc gaccgccaga tcgtggtcgc cctgtgccat cagcggcacg
    16261 atctcgtcgg cggtggaaac ggcggtgagc gtgactctgc agccggtgtc cgtttcgaac
    16321 tgcgtgatcc agtcgaagcg cggatcgccc tcgatgcgtg cgaaatggcg tggccagacg
    16381 aggatatcga cggccccttc cagttcgccg agctcggccg gcagggtgac ggtcgcggcc
    16441 tgcggcggcg gttccttgcc gcatgcggcg agcgtgagtg ccgcggcgca cagcagcagc
    16501 gggccggcct tacttcttgc catggcggga atcctccttg aggcgcacgt agagtgtggc
    16561 gctatcgccc gaggtcacct cgttgacctc gacgtaacgc tgcttgcggg cgagatcgct
    16621 cagcttcgag aagccgtagt tgcgcgggtc gaacgacgga tggttcttca ccagcagcga
    16681 gccgaccgcg ctcaacggcg accagccgtt gtcgcgcgcg gtcgcgtcga tcgccgggcg
    16741 cagcagcttc tcgagcggcg gcaccgaggc ggtgggcgcg gccgactcct gcttggcgtc
    16801 ggcgcgcagg atctcggtga agatgaattt gtcgcaggcg gcgacgaagg cctccggcgt
    16861 cttcttgtcg ccgaagccgt acacctcggc gcccgattcg cgcagccgcg tggcgagcgg
    16921 cgtgaaatcg ctgtcggagg atacgaggca atagccgtcc atgttgccgg cgtgcagcag
    16981 gtccatcgca tcgatgatca gcgacgaatc gctggaattc ttgccgcgcg tgtagccgaa
    17041 ctgctggata ggctggatcg cgaagcggtg caggtgctcc ttccaggcgc tgagtcgcgt
    17101 ggtggtccag tcgccgtagg cgcgcttgac gctcgcagtg ccgtagcgcg cgatctcggc
    17161 cagcagctcc tcgatcagtg acgcctgtgc gttctccgcg tcgatgagca cggcaagtcg
    17221 ttcggtcttg gccgccatgg cctcattatg caccgatatg cgagactccg tgcccgcaag
    17281 cctggaggtg cccgcgaaga tgaatggcga tctttccgga agagtcgcac tcgtgaccgg
    17341 cgcatcggcg ggcctcggtg caaggtttgc gcgggtgctg gccgcggcgg gtgcgacggt
    17401 tgcaatcggc gcgcggcgca ccgaccggct cgccgcgctc gccgcggagc tcacgcaggc
    17461 cggcgcacgc gcgcacgcaa tcgcgctcga tgtcacgcgc caggactcca tccgcgccgc
    17521 actcgaggag accgagcgcc ttgcgggacc cgtcgacctc ctcgtcaaca attccggcgt
    17581 ctccaccgaa acgccgatcc tcgacaccga ggaggccgac tgggaccgcg tgctcgacac
    17641 caacctgaag ggcgccttcc tcgtttcgca ggcggtcgcg cgccggctgc gtgatacggg
    17701 gcgccccggc agcatcatca acatcgcgtc gatcctcggc ctgcgacagg gcagtgcgct
    17761 gacgagctac gccgtgtcga aggcgggact cgtgcagctc acgaagcaga tggcgctcga
    17821 actcgcgcgc taccgcatcc gcgtcaacgc catcgcgccg ggttacatca ggacggacat
    17881 gaatcgcgag ttcttcgatt cggggcgggg cgacgccatg ctgaagcgca ttccgcagcg
    17941 ccgcctcggc gagcccgccg aactcgatgc gccgctgctg ctgctcgccg gcgagtcggg
    18001 cgcattcatg accgggtcgg tgatcgtggt cgacggcggg catctggtga gttcattgtg
    18061 agcgtcgacg ccgggaccga cgtcacgact gcagtgcgcg ccggcatgga gttcgacgtc
    18121 gggcgcctcg agatctacct ggccgatgcc gtcgagggat tccagggtcc gctcaccgtg
    18181 cgccagttcc gcggcggcca gtcaaatccg acttatctgc tggatacccc gcggcgcaag
    18241 tacgtcctgc gacgcaagcc gccggggaag ctgctcgcct cggcgcatgc cgtcgatcgc
    18301 gaataccgcg tgatcgccgc gctcggtgcg cacaccgacg tgccggtagc gcgagcctac
    18361 gcgctgtgca ccgacgatgc ggtgatcggc agctggttct acgtgatgga gcacgttgtg
    18421 ggacgcgtct actgggatcc cacccttccc gaactgcctc gcgaggcgcg acgcggctac
    18481 tacgacgccg cggtggacgc gctcgcgcgc ctgcaccgcg tcgatccggc ggcagtcggg
    18541 ctcgccgatt tcggtcgcgc gggcggctac ctgtcgcgcc agatcgcgcg ctggtcgaag
    18601 cagtaccacg aggacgaggc ggcgggcagc gtgccatcga tggaccggct gatcgagtgg
    18661 ctgccgcaga acctgccggc cgccgagccg cagccggcga tcgtgcacgg cgactatcgg
    18721 ctcgacaacc tgatgttcgc ccccgatggg ccgcgcgtgc tggcgatact cgactgggag
    18781 ttgtcgacga tcggcgatcc ggtcgcggac ttcgcctact acctgatgaa gtaccgcatg
    18841 ccgggcctgg cgctgcccgg gctggcgggt cgcgacctcg acgcgctcgg catcccgtcg
    18901 gagggcgaca gcgtggccgc ctattgcgcg cgcacgggcc gcgagggcct gcccgatctc
    18961 gacttctaca tggcgttctg catgttccgc ctcgccggca tcttccacgg catccgcggc
    19021 agggtgttgc gtggtacggc ggtgagtgcg cgggcgcgcg agtacgcggc caacgtggag
    19081 cctatggcgg atctcgcgtg ggcgcaggcg agtagaggag cgtctcccgg ccgtcgccat
    19141 tgaccgggag gctccgctcg cggacgaggc cgatgtgctc gagcacgcgg atcgagccgg
    19201 cattgtccgg cgagacgatg gcgacgatgc gcgggatgcc gagcgtgcgc gtgccgtagt
    19261 cgagcaccgc ggccgcggcc tcgcgggcgt agccctggcc ccaccaggcc ggcaggtagg
    19321 caaagccgat atccgcgtgt tcgagcgtat cgcgcttcag caggccgcac atgccgatcg
    19381 cggtgccgtc cgtgcgcagc gccacgtgcc acaggccgaa gccatggcgc tcgtagctcg
    19441 ccaccggacc gttcgcgatg taggcgcgcg catcgtcgag gttgcgcacc tcccgatcgc
    19501 cgatgtggcg caggaaggcg ggcacgttga cgagttcgag gatgaacggc gcgtccgcgg
    19561 tcgttaaccg gcgcagcacg agccgcggcg tttcgagcac gcgcaccgtg cgcaatcagc
    19621 cttcgaagaa ccccatcttg acgccggcaa gctggatcac gtcgttgtcc tggagtctcc
    19681 gcgcctggtc cccgatcgcc gagccgttca ccagcgggaa gtcccccggc gtgccgctgt
    19741 cgacgtggac gatgaagtag ccctcggcgc gccgcgtgat ggcggcaacc tgcacgccgg
    19801 ggcgcccgag cgtggtcaga gccttggaga gttcgagttc gcggccggcg aaggcgcccg
    19861 agagcacctg cagcttggcg ggcttgaacg ccgtctggcc ttcggccagg gcgcgacgaa
    19921 ttccggtggc gctcggggcc tgggcggcgc cgtcggcgac actgcgcagc ttctcgacgg
    19981 gacgcgccga gggctggatc accatcgttt tctcgaactc gtcctgctgt tcgtcgtcct
    20041 cgacgaaacg cagctggtgg tggcctaccg tgacgacgtc gccgtgctgg agcgcgtgct
    20101 tcttgatcag cttgccgttg acgtaggtcc cgttggtgct gttcaggtcc tcgaggaacg
    20161 agtcgttgag gatgttgatg atcagcgagt gatgcccgct caccgccggg ttgtcgatgc
    20221 ggatgtcgtt gtcagggagg cgcccgatcg tgtagcgctc cttgttcatg ttgtattccg
    20281 caaggacctg gctgtccagg ctcaatatca agcgtgccat gtgtccgcct tactcagctg
    20341 aaccagccca atactctgtc gaaaacgccg cgacgcgccg gaaaggactc gagtatctgg
    20401 gccatcacga ccgagacgtt gtcccggccc ccgttatcgt tggcgagttg gatcaaatgt
    20461 ttagcgaccg catcaagatt atcactaaag gtattgaccg ttaagtggat atcgctgtct
    20521 tcgaccatgt cgttcaggcc gtcggagcac aggacgtaat gatcgccttt ctggacaggc
    20581 acttcctgca gctcgacctc gacattcgcc tcgacgccca gcgcccgagt cacatagttc
    20641 ttgttggccg cccgctgcgc ctcctccggg gagtagaagc cgcggtcgac cagctcctgc
    20701 agcagcgagt ggtccatcgt gatctgctcg aacttcgggc cgcgcagccg gtagagccgc
    20761 gagtcgccca cgtgggcgat cgaaatcctg ttgtcgaaca gcagcgcact gacgacggtc
    20821 gtgcccatgc cctcgcactg gggttgtgag cgggccgtct ggtagatgat cttgttcgcg
    20881 cgcgtgatcg catcgcgcag gatgatgctc gggcgagtca gcttggtcgt cggatcgatg
    20941 gccgtcatgt cttcgcgctc catcgcctcg cgcacgaggt tgacgatcgt cttgaccgcg
    21001 atcccgctcg ccacctcgcc cgcgttgtag ccgcccatgc cgtcggcgag caccagcagg
    21061 ccgaggtcgg ggtccacggc gatcgtgtcc tcattgtgct cacgcacctt gccggtgtcc
    21121 gtgagcccga ccaggcgcac cttgccgcgc aggctcggcg gtggtccgta acggcgcgcc
    21181 acgactaccg cggccttccc ggcgcgggca gaagcgtgac gcgcgtcaca gacacgatct
    21241 caccctgcgc aatcaaggcc cgcgaacccc cggtcatttg gcaactgaac tcagctgcaa
    21301 acactagcat cggccgccgc caagtccgag tgcctcatca cactatcctt gtgcggccgc
    21361 catccccggt tgcgatccta gagtaagtca cgccttcccg tcagccggcg ccatgcaagt
    21421 cgcgtgctag agtgccgcga tggcccgccc cgtcctcgtc catgtctgca gcgcctgtgg
    21481 cgcggagtcg ccgaagtggc agggccagtg cccgcagtgt ggcgagtgga attcgctgca
    21541 gcagcaggcg gcgccgcgcg cacccgcagc cgcgcgcccc gggcgcggca cggtccgcgc
    21601 ggccccggcg aggctggcgc cggcaggcgc gggcccggca cggtttccga gcgggctcgc
    21661 cgaactcgac cgcgtgctcg gcggcggcat cgtgccgggc tcggtgaccc tgatcggcgg
    21721 tgaccccggc atcggcaagt cgaccctgct gctgcaggtc gcggccagcg ccacggcgtc
    21781 gcgcgccatg ctctatgcca cgggcgagga atcgctggag caggtcgcga tgcgcgcgcg
    21841 ccggctgggc ctcgcgagcg acgccctgtc ggtgcttgcc gaaaccgacc tcgggaccat
    21901 cctcgaggcc gcgcgcaacc tgggcgtcgc gctgctggtc gtggactcga tccagacggt
    21961 ccagttcgcc gaactcggcg cgagcgccgg cgcggtcacc cagctcaagg agtgcaccgc
    22021 gcagctggtg cgtttcgcga agacgagcgg catcgcggtc atgatcatcg ggcatgtgac
    22081 caaggagggc gcgatcgccg gcccgcgcgt gctcgagcac ctggtcgatg ccgtgctgta
    22141 tttcgagagc gaggccggca gccgcttccg gatcgtgcgc gcggtgaaga accgcttcgg
    22201 cgcggtcaac gagctggcct tcttcctgat ggcggaggag ggcctgcggg aagtgcgcaa
    22261 tccgtcggcg atcttcctgg cgcgcgccgc cgaagccgcg ccgggctcga tcgtcatggt
    22321 ggcgcgcgac agaggccggc cgctgttgat cgaactgcag gccctggtcg accggatgcg
    22381 gttcggggcg ccgcggcgcg tcgcacaggg gctcgacgcg aaccgcctcg cgatgctgct
    22441 cgccgtcctg aatcgccacg ccgacgtgtc gctgcaggaa cacgacgtgt tcgcgaacgt
    22501 ggtcggtggc ctcgacctcg gcgagaccgc gaccgatctg ccgctcgtgc tggcgctcac
    22561 ctctagcctg cgcgaccggc cgttgccgca gtcgctggtc gcgttcggcg aagtcggcct
    22621 gaccggggaa gtgcgcccgg tcgcctacgg cgaggagcgg ctgcgcgagg ccgcgaaaca
    22681 gggcttcagg gtggcggtcg tgccgcaggg caacgtgccg cgcaagccca tcgagggact
    22741 cgaagtgcgc ggcgtaggcc gagtgcccga agcgctcact gcggcgttcg cacgcgcacc
    22801 gtcctgatcg cgttgtcctg cacctgcagt acctcgaact gcaggccatc gagccgcagg
    22861 gcggcgccgg catccggaat cgtctcgaag cgctcgagca gcaggccgtt cagtgtcttc
    22921 gggccgtcgg tcggcagggt ccagccgagc gcgcggttca gcgcgcggat cgtcgccgct
    22981 gcgttgacga ggaacacgcc gggcgattca gccgtcacgt ccttgtgcac tacggtcgag
    23041 gtgtcgttgg tgaattcgcc gacgatttcc tccagcagat cctcgagcgt gaccaggccc
    23101 tcgatgtcgc cgtactcatc cacgacgaag cccatccgcc ggcgattgcg ctgaaagtgc
    23161 gtcagctgca cggtgagcgg cgtgccctcc ggaacgtagt acggctcgcg cccgcgcgcg
    23221 agctcctcga ggcgtccgcg cgtcaacgtg ccgcgggcca gctcctgcgc cacgcgcttc
    23281 atgtgcagca ggcccaccac gtcgtcgagt gtgccgcgat agaccggcag gcgcgtgtgg
    23341 ggtgtctggc ggatctgggc aaggatgtcg tcccagtcgt cgtcgaggtc gatgccggcg
    23401 atgtcctggc gcgggatcat gatgtcgttg acggtgatct gcccgagatc gaggatcgaa
    23461 agcagcatcc ggcggtggcc ctgcgggatc acggtgccgg tctcggcgac caccgtgcgc
    23521 agctcgtcgg ccgagagcgt ctgtccgccg cgattcgcgc gcctgacacc gaagaggcgc
    23581 aggaacccgt acgcgagctg gttcgtgatc cacagcagcg gcttcgcgac ccacaccagc
    23641 gcgcggtaga tgtaggtgct gccgagcgcg accggctcgg gatggcgcgc ggcgtagatc
    23701 ttgggcgcga gttcgcagaa cacgatcatc acgatcgaca gcgcgatgat ggtcaccggt
    23761 accgcccatt ccccgccgta gcgctgcgcg agcagcgtcg cgatcgacga cgccgcgacg
    23821 ctcgcgagcg ccaggatcac gaggtttgcg ccgagccagt cgtcgggcct ggcgagcagc
    23881 gagtcgagca ccctggcggt gcgatggccg cctgccgcac ggtggcggac gcggtagcgg
    23941 ttgacggcga gcatcgcgac ctccgtaccg gaggagaatg cgatggtggc gagcagcgcg
    24001 acgagcgcaa tgaccagcac ggtcgtgggg gcggtatcca tggggcccaa gaatagggcg
    24061 cggcgcccga tcaggcacgg gggcccgctc ggcgacgccg tcacgtaaac ttgcgggcac
    24121 catgttcgat aacctgacag cgagactgtc ccgcaccgcc gatgccctgc gcgggcgcgg
    24181 gcgcatcacc gaagagaaca tcgccgagac cctgcgcgat gtgcgcatgg cgctgctcga
    24241 ggccgacgta gccctgccgg tcgtcaagcg cttcatcgag aacgtgcggg cgaaggccct
    24301 cggcgcggag gtgcagcaga gcctcacccc cgggcaggtc ttcgtcggca tcctgcacaa
    24361 ggaactcgtc gcgctgatgg gcggggcggc ggccggtttc agcctgcgcg cccagccgcc
    24421 gttcgtggtg ctgctcgcag gcctccaggg cgcgggcaag accaccaccg cggccaagct
    24481 cgcgcgctgg ctgggcgaga cgcacaagaa gcgcgtcctg ctggtctcga ccgatgtgcg
    24541 ccgcccggcg gccatcctgc agctggagcg cctggccgcg caggtcggcg caggcttcta
    24601 caaggccgac ccggcaaccc cgccggcccg gatcgccgcg caggcgctcg agcaggcgaa
    24661 gcgcggcctg tacgacgtgc tgctcgtcga taccgccggg cgcctgcacg tcgaccagga
    24721 actgatgcag gaggcgcgcg agatcgatct tgccgtgcag tcgcacgagc ggctgttcgt
    24781 ggtcgatgcg atggccggcc aggacgccgt gaatgcggcc cgggccttcg gcgaggcgct
    24841 cgatctcacg ggtgtgatcc tgaccaaagc cgacggcgat gcccggggcg gcgtcgcact
    24901 gtcggtgcgc gaggtcaccg gccggccgat cgtgttcgtc ggcgttggcg agaagaccga
    24961 ggccctcgaa cccttcgatc ccgagcgcat ggcgaaccgc atcctcggca tgggcgatgt
    25021 cgtggcgctc gtcgagcagg tgcagaaaca ggtcgatgtc gacgaagcgc agcgcctcgc
    25081 ccgcaaagtc gccaagggca aggccttcga tttcaatgac ctgcgtggac aactggaaca
    25141 actgctgaag atgggcgggc tggccgccgt catggacaaa ctgccccagc acatgacccg
    25201 ggccgcagcc gcgaatgcac cgggcgacag ggacgtgcgc cgccagatcg ccatcatcaa
    25261 ttcgatgact cggcgcgagc ggcgccagcc ggccgtgatc gacggctcgc gccggcgccg
    25321 cattgctgcc ggggcggggg tccaggtaca ggacgtcaat cgcctgctga agcagttcca
    25381 ggagatgcag cgaatgatga aaagcatgaa aggaggccgc ctggcgcgca tgctcggggc
    25441 catgaaaggc gggcgtccgc cgggcttcct ataagccgct gatttgcgta gaatccgcgc
    25501 tcttttttgg ccgccccggg cggggcggcg agcaaacggg aattatggtc acgattcgtc
    25561 taacgcggcg cggtgcaaaa aacgcgcctt tctatcatgt ggtggtcacg gacagccgca
    25621 agcgccaggg cggcaagagc cttgagcagg tcgggttttt caacccgatc gcgcgcaagg
    25681 gcgacaccaa gctgaagctc gatctgcccc gcatcgacca ctgggtcagc aagggtgcga
    25741 agccttcggt gcgtgtcgcc acgctggtct cggactaccg caagcaagcg gcggcctgac
    25801 gactacggca ccgcgggtcg agatcgggcg catcggtgcg ccttacggcg tgcgcggctt
    25861 tagccacgtc gactcgttca ccgatccgcc ggagctgttg tcggaatttc gctcctggcg
    25921 gctgcgcaac gcgcagggcg cggtggcgga gtacgaggtg gccgagatgc gtgctcatgg
    25981 ggcgcgcttc gtggctcgcc tcgtcggggt cgactcgcgc gaaggcgcgg caaggctgac
    26041 gggcgccgtg atcgaggtcg agcgcgacgc gctcccggac cccggcgaca ggcagcacta
    26101 ctgggtcgac ctgatcggcc tcgc
//
LOCUS       DAOQVY010000025        24922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_2336_length_24922_cov_5.811638, whole genome shotgun sequence.
ACCESSION   DAOQVY010000025 DAOQVY010000000
VERSION     DAOQVY010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 24922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24922
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2336_length_24922_cov_5.811638"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PKN91_04010"
     CDS             <1..722
                     /locus_tag="PKN91_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNS27268.1"
                     /translation="VAVAHVSNALGTVLPVKDIVAAAHARGIPVLIDGAQAVAHGGAD
                     MQALDCDFYAFSGHKLYGPTGIGVLYGREALLREMPPWQGGGDMILTVTFAGSTWNEL
                     PWKFEAGTPNMSGAVGLAAAIDYLEGLGPDAVHAHEQRLLARATAAVQGIPGLRIIGT
                     ARDKVAVLSFTVEGVHPHDLGTILDHEGVAIRTGHHCAMPVMDFFEVAATARASFACY
                     NDDADVDRLIAALHAAREVFA"
     gene            722..1171
                     /locus_tag="PKN91_04015"
     CDS             722..1171
                     /locus_tag="PKN91_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNS27269.1"
                     /translation="MDLKELYRDVILDHNRQPRNFGRLVPADASADGYNPLCGDRLTV
                     YVNLDGERIRDVRFDGKGCAISVASASLMTEAVAGKDRGEVARLFDAVHAALTGEDAD
                     RAAAVPLGKLAALSGVREFPARVKCASLCWHTLNAALAHERTPVTTE"
     gene            1175..1726
                     /gene="sufT"
                     /locus_tag="PKN91_04020"
     CDS             1175..1726
                     /gene="sufT"
                     /locus_tag="PKN91_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HNS27270.1"
                     /translation="MRTHQNEPFVVQRAVNAIMVPAGIEVKLKPGQAGYITQALGGSF
                     TLYIDGNLFRLPGGEADAIGHEVVKPPELPPDATLDDLRKVIREQMATCYDPEIPINI
                     VDLGLIYSCDVQPNGDGTHDVNVSMTLTAPGCGMGEVLVQDVKDKVEIIPTIRRANVE
                     LVFDPPWNQTMMSEAARLQTGMF"
     gene            1766..2272
                     /locus_tag="PKN91_04025"
     CDS             1766..2272
                     /locus_tag="PKN91_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNS27271.1"
                     /translation="MKLLTLVRHGQAEWKASKLADFDRPLTRRGLAEVTDMARRLRVR
                     ELPSPDLILTSAALRTVQTSEVFAKELGVSPRLVKLLDTLYLAAPEVLLEEIAGMGPR
                     VAHLLVVGHNPGLSDLAVQLAPDAGLASFATGACCTLTFDSRSWQEPGRASSAEYDAP
                     GRFFDLWH"
     gene            2351..4168
                     /gene="typA"
                     /locus_tag="PKN91_04030"
     CDS             2351..4168
                     /gene="typA"
                     /locus_tag="PKN91_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNS27272.1"
                     /translation="MRPLRNIAIIAHVDHGKTTLVDKLLHQAGTFAAHQHIAERVMDS
                     NDIERERGITILAKNTAVDYAGTHINIVDTPGHADFGGEVERVLSMVDGVLLLVDAVE
                     GPMPQTRFVTRKALALGLKPIVVVNKIDRPAARADWVVNQTFDLFDKLGATDEQLDFP
                     IVYASGLNGWATLEAGAQGTDMRPLFDTILAHVPAPAGDPSGPLQLQISALDYSSFVG
                     RIGIGRVRRGTLHAGDDVLLLDGDKAPVRSRVNQIFGFRGLERVPLEAAAAGEIVLIN
                     GIDDIGIGVTLADVERPEALPALRVDEPTLTMNFRVNNSPLAGTEGKFVTSRQIRDRL
                     TRELLTNVALRVSDTEDTDVFLVAGRGELHLTILLENMRREGYELAVSRPRVVVREVG
                     GERLEPYEILTVDVDEAHQGGVMEALGQRRGELQDMASDGRGRVRLDYRIPARGLIGF
                     QGEFLRLTRGTGLMSHVFDEFAPMKDDVPARRNGVLISAEAGTAVAYALWKLQERGRM
                     FVTPGEKIYEGMIIGIHSRENDLVVNPAKTKQLTNIRAAGKDDAILLTPPIELTLEAA
                     VEFIDDDELVEVTPQSIRLRKRVLKESDRRRAARTGDAA"
     gene            4228..5520
                     /locus_tag="PKN91_04035"
     CDS             4228..5520
                     /locus_tag="PKN91_04035"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNS27273.1"
                     /translation="MPSRPRLVTLCAALLVAPIFLSAAQAATPTIEERLQALEAGYAQ
                     LKEENAQLRALIAAGTGAGTPAPAIAPETAAPVVSVAPANNDAPAQVLAAGKVARLTL
                     GGFVQAQYEAGDAGDARFVGVNDRFYIRRGRVSVTGAFAEHFDFRVEGDFGANSHSAA
                     SGLRVSANEIYVNWNKYPAANLRIGYLKPAFGAEQLASDPTTPTIERFLASDRIADGR
                     QPGLGLYGSFLDKRLGYTVTLGNGNGSNSSLNDNDGFLGAARVYGTLFESSRAGRLTG
                     GINAMRSDDALVLRPGFGFDLNGSGLPDAIFRGERSGWGIDARWSLGRTTLSGELLRE
                     NFKPDNATPAADFDAQGWHLTATYMLLPQRLEAVLRREVFDPNLDVAGDATENWVGGL
                     NYLIKGSDIRLMINYMFGDAPNLPDDSGRLLARMQIVY"
     gene            complement(5558..6667)
                     /locus_tag="PKN91_04040"
     CDS             complement(5558..6667)
                     /locus_tag="PKN91_04040"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HNS27274.1"
                     /translation="MIDRAALLKLHRWVGLTVGVLLFVQGLTGTLLVFREEIEHLLYP
                     ELVVTPAATAVPVQQLVDTVRAATPDATLQRIRFAEGAAAALFVMGGEDDRPRLVAVD
                     PWRGEILRQGGYARWPTELLFRVHDELLSGRTGEIIVSIEGIALAFLVIAGFILWWPG
                     RRRLRSGFRVITGQGADRAVRTTHRALGGAIAVVLLMSGLTGALLIHRATLQSVLPMV
                     PRPKFVVPEQAGQPLMAIDTLLSRARSEHGPLPLRELRFSGSKGQVIAYYFQDPSSLR
                     PNATRQYFYNAYDGSELGRYEPAKLPLLNTAYDWLYTIHTGRAARAPGRVLVLTGGLS
                     LVFFVTSGAWLWLSARQQRHARRMKAAARLAREPR"
     gene            complement(6675..8102)
                     /locus_tag="PKN91_04045"
     CDS             complement(6675..8102)
                     /locus_tag="PKN91_04045"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNS27275.1"
                     /translation="MPGVFRHAILCAALAWVGQASAAAADSDAARDGENRFIALYREL
                     VETDTSFSSGSCTLAAERMAARLVAAGYPGADVRVIVPPGFPRQGNLLARVPGTDARL
                     PAILLLAHIDVVEAKASDWKRDPFTLVEENGYFHARGAVDDKAMAAAFVDAFIRYREE
                     RFRPRRTLKLALTCGEETDAVFNGVQYLLANEPEALAAGFAINEGGSGRLDANGQPLS
                     FGVQVGEKIYQDYTLTATSPGGHSARPTDDNAIGRLAAAVARIDGYRFPVHMTAATQA
                     YFRHSAALYVGQMASDLVAAGSATADASALARIAAADPLWNAFLRTTCIPTLISGGHA
                     ENAQPQHAEANVNCRIMPGESVEDVRDRLVEIAADTKIAITLADEPGPQPVAPPLEVR
                     IMRPVEAIVADMWPGVPVIPSLSSGATDGRFLNAAGIPTYGISGVFRDPDGNGVHGLD
                     ERIRVRSLLDARAFLHRLVRAYASE"
     gene            complement(8186..9574)
                     /locus_tag="PKN91_04050"
     CDS             complement(8186..9574)
                     /locus_tag="PKN91_04050"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNS27276.1"
                     /translation="MRHNLRVSLTARLLVALILPTAALALVLGVGGALFIGRIVESVN
                     DRPLDASVRSIADTLALEDGEVTLDLPPSAFGMLENEERDNVYYSVHVGDELLTGYPD
                     LPRIGDANARIDETRFAYSTYRDTPIRVATIERRVPRVEAPIIVQVAETLDGRKSLSR
                     RMLGGLFALEALLIGLLVLLLPIAVRWGLFPLAEVRRGLDSRSATDFTPLTLQQVPVE
                     LRSFVTAFNGLLLRLHAAVEGIRSFTTDASHQMRTPLSILRAHLQVLKRQGLQTKEGQ
                     ASLADIDVATARLQRLLTQLLALARAEGNRESVARLGRPVDLAALARSVAEEYVPAAL
                     RDGVDIQFDTDGRTTVRTVAEFARELVSNLMDNAIRYNRRGGTVWLRVSALQDAVRIV
                     IEDDGPGIPQEMQADVFKRFRRLDRDRAVEGSGLGLAIVRELADALAASVRIASRPGG
                     GLRVEIDFPGVA"
     gene            complement(9564..10247)
                     /locus_tag="PKN91_04055"
     CDS             complement(9564..10247)
                     /locus_tag="PKN91_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNS27277.1"
                     /translation="MARILLVEDDEALARGLVAILRGEGYSVDPAHDGESALAMARDE
                     PYAVVMLDINLPGMSGFDVLKTLRARGCHAPILVLTARDSLDDRVRGLDFGADDYLLK
                     PFEPPELSARLRALLRRSAADPSPVIRVGNLEIDRSRRVIRVAGEPVDLRRREWVVLE
                     RLVVRVGKVVSKERLAAEVFGFDDIVAPNAIEVYIARLRRKLGPHGPTIRTMRGLGYV
                     MEDSPAGEA"
     gene            10371..11420
                     /locus_tag="PKN91_04060"
     CDS             10371..11420
                     /locus_tag="PKN91_04060"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNS27278.1"
                     /translation="MLLSAATAVAAAAQPRGYPRSYDGIITAAQREGVVVVYSTTDEH
                     EVVGALGAFRERYPFLRVDYHELESPDLYARVIAESRARRPTGDLIWSSAMDLQIKLV
                     NDGYAQAYASPEKPWLPDWAIWKNEAWGVTAEPVVIAYNKRLVPAKDVPTTHAAFERL
                     LRSRPEFYRNRVSTYDPRLSGFGYLQLAQAYHVSRDLMPLTGALGAVDVKLHTSSRPL
                     LADIESGRSLFAYNALGSYALELATRNPDIGVIMPRDYTLVVSRIALIAREARQPNAA
                     KLLLDFLLSAEGQGHLARAYMAPSREDVEPLPGSKPPAAAARPVRLGPALLADLDRIR
                     RERFLARWQAALEAR"
     gene            complement(11428..12741)
                     /locus_tag="PKN91_04065"
     CDS             complement(11428..12741)
                     /locus_tag="PKN91_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HNS27279.1"
                     /translation="MLSILAFGMVATFMTLIMTKRLSAMIALIIVPAVFALFAGFWSG
                     LGDMMLAGIRDIAPTGVMLLFAILYFGIMIDAGLFDPLTSRVVKLAHGDPLRITMGSA
                     ILGLVVALDGDGTTTYMITLSALLPLYKHMKMDIRVLTCILIMAVAAMNLVPWGGPTT
                     RAAIALGVDPRELFLSLIPPVLAAVGWVLFTAYVLGRAERKRLGITERMAMPSPGELD
                     LSMPDSDGTPSTRRPRLFWFNAALTALLMGILLFGDVPLQVLFMLAFAIAAMVNYPQV
                     EMQRERVAAHASNALATAGLVFAAGIFTGVLSGTKMVDAMANTVTGVVPDALGPYMAS
                     ITALVSIPFTVLISNDAFYFGMLPVLAQSAEHYGISTMEIARASLVGQQIHLLSPLVA
                     STYLLVATAGVELGDHQRFTMKWALGACAVFMLDCLLFGLFPWHA"
     gene            complement(12818..15373)
                     /gene="acnA"
                     /locus_tag="PKN91_04070"
     CDS             complement(12818..15373)
                     /gene="acnA"
                     /locus_tag="PKN91_04070"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HNS27280.1"
                     /translation="MNAAPGTGSSGPGPTRVDLPGLLGARLDRMPWVHRILAENVLRH
                     RHAIADADRAAGRWLEQGDCATELPFAPSRLMMHDTTCGPALVDLAAMRTVLAARGGD
                     PLRVRPGLRVDVSTDHGISVDRYGVPGALAANMAIEMQRNAERFSLMKWAAQAFDTLR
                     VHPPGSGIMHTLNLERLASVVEVATWQGREWIFPDTLIGTDSHTPMIGGIGVLGWGVG
                     GLEAESVMLGVPVSLRVPEVVGVRLSGTLRSGVMATDLALEVTHLLRDVCVDGRFVEF
                     FGSGVATLSAGTRAVVANMAPEFGGATGYFPIDEQVLTYLRTTGREHSHIERVERYAR
                     CQRLWFDADAQPDYECVIDLDLAAVAPGIAGPRRPQDRIDRRATAATGGGMRPVAVAA
                     ITSCTNTSDPRLLVAAGLLARRAVVCGLSVQSWVKTSLAPGSPTARLMLERAGLLAPL
                     EALGFAIVAYGCTTCIGNSGPLAPGIERLGSGVRPVAVLSGNRNFPGRVHPRIEDSYL
                     ASPPLVVAYALAGDFDRNIETDPLGHSPDGTPVHLHDIWPDDAEIDAVLALATDPADF
                     GPAYDAAEASPAWSALAAPRGELYPWDRNSTYLRLPPFVDPDARTQLGRYLAQPLLVL
                     GDDVTTDHISPAGAIAASGDAGRWLIEHGANPDDLNVFAARRGNWQVMLRGLYTNVSV
                     RNLLRADIPPGATVHVPSGEILALWRAAQRYADSGVPVVIIAGERYGTGSSRDWAAKG
                     LRLLGVRAVLAASFERIHRSNLCHMGILPIELPAGMSPGSLALAPGDRIEIDMPADAL
                     VPSAPCVVGTRYQDGRTHRFTARAAVETLAEVAALRAGGLLPLMLRRLSLTAS"
     gene            complement(15370..15819)
                     /locus_tag="PKN91_04075"
     CDS             complement(15370..15819)
                     /locus_tag="PKN91_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNS27281.1"
                     /translation="MVLTPGRPVVLRVAQRPATDRGGGILTTPMVTARVGSRQMLNGI
                     TVLAPGAAVPLHFHNCEESVLVLSGHGRAHVDGKAHDVGPGDVSWIPAGIAHFFRNTS
                     ADERFEIFWTYASVDATRTIVATGVTTRIDEEHGPADLRASATDGPA"
     gene            complement(15823..16887)
                     /locus_tag="PKN91_04080"
     CDS             complement(15823..16887)
                     /locus_tag="PKN91_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNS27282.1"
                     /translation="MKLLLLPGDGIGPEIAAVTRRVLEALADTCALAIAIEERAVGLA
                     SLAECGTTLPDLVLARIPEVDGVILGPVSHADYPPREAGGINPSGALRKQFDLYANIR
                     PCRSRPGLTMLRRPMDLVIVRENTEGFYSDRNMYAGNGEFMPDQDMALSVRKITAQGV
                     SRVARIAFELARERRGKLAAVHKANVLKLTDGLFLREVRKVATQFPGVEIEELIVDAT
                     AALLVRNPDRFDVILTTNMFGDILSDEASELSGSLGLGGSINAGDDICVAQAQHGSAP
                     DIAGRDIANPSSLLLSCAMLLDWIGRRSGRSDLLRAARVLEGALERVLADPGSRTRDI
                     GGTLGTSAFGEVVCGAVRAG"
     gene            complement(16972..19179)
                     /locus_tag="PKN91_04085"
     CDS             complement(16972..19179)
                     /locus_tag="PKN91_04085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HNS27283.1"
                     /translation="MSILWNRIAALGLGGFAMVAASQADAQEPAAEDARSGPVLEEIV
                     VTSDRPDSYGADFVQAGSFRGARQLDTPLTVSVISEQVLKSQQAQSILDALRNSPGVT
                     SSQTSPTVYNNLSIRGIPVENRGNYRLNGSLPIVNLIDLPLENKVRVEALKGASALYY
                     GFTTPSGIINLTTKRPTVDPMLSVAVNGNDFGQIQGHLDASGTIGALGLRGNFVHGEI
                     DPGIDNVSGDRTFLSGALQYELTDSLRFNLDVEHIEKEMTEPTVVQGPTSAANLLTVL
                     PRLPRASNNPGSEGFMNRAEEDNVLGRLSWKINSAWDLTVEGGLSYAERDRRFSSLGN
                     FNPVTGLGTLTMSAADGQLYRNRNGRIELAGALATGQVVHELLVGASKNIRRQYSAPA
                     VARVGTGSAATRNNCVLLGLTTTCLQSLYDPVDLADIRFDGTATYNAARDTSIEDTGF
                     YAFDRMRFGGPNDDMFSILAGVRLSDYKEEQRTATGPAIETFSDDPVSLSGGIVYKPV
                     EWASIYGTYIEGLESTPAAPLAANNAGEILPASDSTQYEAGVKLEPRKGLLFTAAYFD
                     ITRELTYTNAANFFVKDGEATYRGFEFSVTGEITPDLSVYASGLSLRAKQGETANAAL
                     IGKRIENTARTQWSVAAEYRLTRFIDGLAINAGVYYTGDRAINPQNSLFVPSYTLYDL
                     GGSYRFQVGGVGMTVRVNAQNITNERYFASTSTNYFAFGTPSTVKLSLTADMF"
     gene            19405..20970
                     /locus_tag="PKN91_04090"
     CDS             19405..20970
                     /locus_tag="PKN91_04090"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNS27284.1"
                     /translation="MTAADPHRISRRRFTGMTLGGTAGLLLPNLAACRSPAVPAASAA
                     DGLPRNRPEAQGVDPRGILAFLDEVRAEGLELHSFMLARGGDVIAEGWWSPYRPDLPH
                     MMHSLTKSVAATGVALALQEGLFGIDDKVISFFPDELPAEVSDNLAAMTVRDLLTMRA
                     GHAEEISGSVWRQIRTSWVTEFFKVPVPLKPGTKFVYSSAVSFMLSAIVTKLTGQTLR
                     DYMEPRFFKPLGITGLSWDVGPGGINPGGNGLTWKTADALKLGMLHAQNGRWNGVQIL
                     SPEWVREASRPQVAEGEYGYQWWIGPNGAYYALGLFTQMSIVFPQHDAVLAVTAAIDG
                     SEHLAQIVWKHFPQAFGAGSRPRDDAAHATLTRRTASLSVQPELSAGSSPTASRISGQ
                     RFRAAANAEGIEDFRFTFDGDRCRFTVRDGRGEHNVDVGVGRWIEGRTSMTGNKLHHQ
                     YQPDSMFVVAGGQWTAPDTFEMTWQFAETAFRDTVVCRFMDDRMTFDRSVNVNSAARS
                     LPTVQATVLGAAE"
     gene            21118..22620
                     /locus_tag="PKN91_04095"
     CDS             21118..22620
                     /locus_tag="PKN91_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27285.1"
                     /translation="MTLPGYLVDTDHGTRVCVPFTTVGARPPQGYSGDFYVDEFSDEA
                     LRARWLECRTDAACSARVTRVVERRLPPNRESRITDPHHRYLLGKIAEAPNLDLRSIR
                     RPGFFARAPYREPIAAAERVTSTVEFTAPAEAYERAHGIDQPIKLRGWYLRGRGVLDA
                     MGKRRRALLVMSGGGGTRLVAIEDPSDRLYSVDPATGKTSINAFPNATTGSPGQAGWR
                     AIMAMFNEAGFDVLAYDRRGVGVSSGYSDTNTLQQGRDLLEALASLRSGDGLRVLTPK
                     GTVLNGAAAAEALRGGTGELPTLLLGNSRGTMASGWAMTINFHRDCSYDQPEIVCAPP
                     RRDESIKGALLMAEFTSGPGFVPATPTREDEGRGLGKDRGLFIGASEVEHQIIFFPGS
                     AILAGMRAWPAAFFVRGLWDYAASLEGTIASYDRVPGLKELVVVRGPHPYETWPEVEK
                     ERVRARMLAFARAVIRGDGEVPGARKWTTMRELVETTEDVWERSSAPRAP"
     gene            complement(22630..23676)
                     /locus_tag="PKN91_04100"
     CDS             complement(22630..23676)
                     /locus_tag="PKN91_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNS27286.1"
                     /translation="MLIATAMLALLAAAPDTTSAQDGRGAASSFSRSADTREGALDSF
                     TNPGTDDAAALLKHLQQEIYVTQQFAILTQFRLDHADRIRLSTVFVNSDGELIPTHVF
                     TPLKEDAGTRRPAVVMVHGGFHERLEPSWFPLIEAVVEAGYVVLFPEYRGSRGYGDAI
                     YQNDYGSTDVADVLAAGRYAAGRPDIDPARIGILGQSRGGMVTLLAIERAPKQFRAAV
                     DIVGLTDFVAYMSYKPDYRRKEIAETNPGFKGKLPHENLPAYIDVSPINFVDRIEAPV
                     LVLATSGDKIVPHTLHTGRLIDALKANGKTYEAKIYDNAPGGHVFMKGATPERDDALQ
                     RIVAWFDRWLGAAP"
     gene            complement(24170..>24922)
                     /locus_tag="PKN91_04105"
     CDS             complement(24170..>24922)
                     /locus_tag="PKN91_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNS27287.1"
                     /translation="AAARELTRAHGSLLLVDSIQAGLRAHGVLSIVDYPGFEGLDAPD
                     METWSKALNAGQYPLSVLAVSEQVSRLYRAGIYGNTMTTNPRALDVACAVLGALTPEL
                     RANIRDRGREAIRKLEQLKAELGGAITKVQGTGLLFSCELAPQYKCYGAGSTEEWLRE
                     RGIGVIHGGANSLRFTPHFALAADELDLLVSMVGKALREGPKYAQAAAAQGQGARARD
                     AGGDEIRRSRQSLSAREMPASPASRRSASGQP"
BASE COUNT         4292 a         8584 c         8113 g         3933 t
ORIGIN      
        1 tggtcgcggt ggcgcacgtc tcgaacgcgc tggggaccgt gctgccggtg aaggacatcg
       61 tcgcggcggc gcatgcgcgc ggcataccgg tgctgatcga cggcgcgcag gccgtcgcgc
      121 acggcggcgc cgacatgcag gcgctcgact gcgacttcta cgcattttcc ggccacaagc
      181 tctacggccc taccggcatc ggcgtgctgt acggccgcga ggccctgctg cgcgagatgc
      241 cgccgtggca gggcggtggg gacatgatcc tgacggtgac tttcgcgggc agcacctgga
      301 acgagctgcc gtggaagttc gaggcgggca caccgaacat gtccggcgcg gtcggcctgg
      361 cggccgcgat cgactatctg gagggtctcg gcccggatgc cgtgcatgcg catgagcagc
      421 ggctgctcgc cagggccacg gcggcggtgc aggggattcc cggcctgcgg atcatcggca
      481 ccgcgcgcga caaggtcgcc gtgctctcgt tcaccgtgga aggcgtacat ccgcacgacc
      541 tcggaacgat tctcgaccat gagggggtcg cgattcgcac cggccaccac tgcgcaatgc
      601 cggtcatgga tttcttcgag gtcgcggcca ccgcgcgcgc ctcattcgcc tgctacaacg
      661 acgacgccga cgtcgaccgg ctgatcgcgg cgctgcacgc cgcgcgcgag gtattcgcct
      721 gatggacctg aaggagctgt accgcgacgt gatcctcgat cataaccgcc agccccgcaa
      781 cttcggccgg ctcgtgcccg ccgacgcgag cgccgacggc tacaacccgc tgtgcggcga
      841 tcgcctgacc gtctacgtga atctcgatgg cgagcggatc cgcgacgtac gtttcgacgg
      901 caagggctgt gcgatctcgg tggcctccgc gtcgctgatg accgaggcgg tcgccggcaa
      961 ggaccgcggc gaagtcgcac gactgttcga tgccgtgcat gcggcgctca ccggcgaaga
     1021 tgccgatcgc gccgccgcgg tcccgctcgg caagctcgca gccctgtccg gcgtgcgcga
     1081 gttcccggcg cgcgtgaagt gcgcgagcct gtgctggcac acgctcaatg cggccctcgc
     1141 gcacgagcgc acaccggtca cgacggagta atcgatgcgc acgcaccaga atgagccgtt
     1201 cgtcgtgcag cgcgcggtca atgcgatcat ggtaccggcc ggcatcgagg tgaaactcaa
     1261 acccggccag gcgggttaca tcacgcaggc gctcggcggc agtttcaccc tgtatatcga
     1321 cggcaacctg ttccgcctgc ccggcgggga agccgatgcg atcgggcacg aggtcgtgaa
     1381 gcccccggag ctgccacccg acgcgacgct cgatgacctg aggaaagtga tccgcgagca
     1441 gatggcgacc tgctacgacc cggagatccc gatcaatatc gtcgacctcg gcctgatcta
     1501 cagttgtgac gtgcagccga acggcgacgg cacgcatgac gtcaacgtct cgatgacatt
     1561 gactgccccg ggttgcggca tgggcgaggt gctggtgcag gacgtgaagg acaaggtgga
     1621 gatcatcccg acgatccgca gggcgaatgt cgagctggtc ttcgacccgc cctggaacca
     1681 gaccatgatg tcggaggccg cgcgcctgca gaccggcatg ttctgacagg agccgcgcca
     1741 tgactgcaag catcgagctg ccgggatgaa actgctgacc ctcgtccggc atggccaggc
     1801 ggagtggaag gcatcgaaac tggcggactt cgaccgaccc ctgacacgtc gcgggctcgc
     1861 cgaagtcacg gacatggcgc ggcgcctgcg cgtcagggaa cttccctccc ccgacctgat
     1921 cctgacgagt gctgcactgc gtaccgtgca gacgagcgag gtgttcgcga aggagttggg
     1981 cgtctcgccg cggctcgtca agctcctcga caccctctac ctggcggccc cggaggtgct
     2041 gctcgaagag atcgccggga tggggccacg cgtcgcgcac ctgctggtgg tggggcacaa
     2101 ccccgggctc agcgaccttg ccgtgcaact cgcgcccgac gccggcctcg cgagtttcgc
     2161 cacgggtgcg tgctgcacgc tcaccttcga cagccgcagc tggcaggaac ccgggcgcgc
     2221 cagcagtgcc gaatacgacg ccccgggacg cttcttcgac ctctggcact gagttcccgg
     2281 ccggccggcg ccggtatggt agactccgcg accttttttt tccgcccacc cagggcaccc
     2341 ggaccctcgc atgcgcccgc ttcgcaacat cgcaatcatc gcccacgtcg atcacggcaa
     2401 gaccacgctg gtcgacaagc tgctgcacca ggccggcacc ttcgccgcgc accagcacat
     2461 cgccgagcgc gtgatggact cgaacgacat cgagcgtgag cgtggcatca cgattctcgc
     2521 caagaacacc gcggtcgatt acgccggcac ccacatcaac atcgtcgaca cgccgggcca
     2581 cgccgacttc ggcggcgagg tcgagcgcgt gctgtcgatg gtcgacggcg tgctgctgct
     2641 cgtcgacgcg gtcgagggcc cgatgccgca gacccgcttc gtgacccgca aggcgctcgc
     2701 tctcggcctg aagccgatcg tcgtcgtcaa caagatcgac cggccggccg cgcgcgccga
     2761 ttgggtcgtc aaccagacct tcgacctgtt cgacaagctg ggcgcgaccg acgagcagct
     2821 cgactttccc atcgtctacg cctccggcct caacggctgg gcgacgctcg aggccggcgc
     2881 gcagggtacg gacatgcggc cgcttttcga cacgatcctc gcgcatgtgc cggcgccggc
     2941 gggcgatccg tcaggcccgc tgcaactgca gatctcggcc ctcgactact cgagcttcgt
     3001 cgggcgcatc ggcatcggca gggtgcggcg cggcaccctg cacgccggcg acgacgtgct
     3061 gctcctcgac ggcgacaagg cacctgtgcg ctcgcgcgtc aaccagatct tcggctttcg
     3121 cggcctcgag cgcgtaccgc tcgaagccgc ggcggccggc gaaatcgtgc tcatcaacgg
     3181 tatcgacgac atcggcatcg gcgtcacgct ggcggatgtc gagcggcccg aggcgctgcc
     3241 ggccctgcgc gtcgacgaac cgacgctgac gatgaacttc cgcgtcaaca actcgcccct
     3301 tgccggtacc gaaggcaagt tcgtcaccag ccgccagatc cgcgaccggc tcaccaggga
     3361 gctcctgacc aacgtcgcgc tgcgggtctc ggataccgag gataccgacg tgttcctggt
     3421 ggcgggccgc ggtgaactgc acctgaccat cctgctcgag aacatgcggc gcgaaggcta
     3481 cgagctcgcc gtgtcgaggc cgcgcgtcgt cgtgcgcgaa gtcggcggcg agcgcctcga
     3541 gccgtacgaa atcctcaccg tcgacgtcga tgaggcgcac cagggcggcg tcatggaggc
     3601 cctcgggcag cgccgcggcg agttgcagga catggcatct gacggccgcg gccgcgtgcg
     3661 cctcgactat cgcattccgg cgcgcggcct gatcggcttc cagggcgagt tcctgcgcct
     3721 cacccgcggc accggcctga tgagccatgt gttcgatgaa ttcgccccga tgaaggacga
     3781 tgtgccggcg cgccgcaacg gcgtgctgat ctcggcggag gccggcaccg cggtggccta
     3841 tgcgctgtgg aagctgcagg agcgcggccg catgttcgtg acgcccggcg agaagatcta
     3901 cgagggcatg atcatcggca tccacagccg cgagaacgac ctcgtcgtca atccggccaa
     3961 gaccaagcag ctgaccaata tccgcgccgc cggcaaggac gacgcgatcc tgctgacccc
     4021 gccgatcgag ctgacgctcg aggcggcggt cgaattcatc gacgacgacg aactggtcga
     4081 ggtcacaccg caatcgatcc gcctgcgcaa gcgtgtgctg aaggaaagcg atcgccggcg
     4141 cgcagcgcgg acgggcgacg ccgcataatc ttcggataac gcgggacacg gcataatgtc
     4201 cgcggtttct caccgcagga cgagcccatg ccctcacgcc cccgcctggt cacgctgtgc
     4261 gccgcgctgc tcgtggcgcc cattttcctc tccgctgcgc aggccgcgac gccgacgatc
     4321 gaggagcgcc tgcaggcgct cgaagccggt tacgcccagc tcaaggagga gaacgcccag
     4381 ctgcgagccc tgatcgccgc aggtaccggg gccggcacac ccgcgccagc aatcgctccg
     4441 gagactgcgg caccggtggt ctcggttgcg cccgcgaaca acgacgcccc tgcgcaggtg
     4501 ctggccgcag gcaaagtggc caggctgacg ctcggcggct tcgtgcaggc tcagtacgaa
     4561 gcgggtgatg ccggagacgc gcgcttcgtc ggcgtcaatg accgcttcta cattcgccgc
     4621 gggcgcgttt cggtcaccgg cgcattcgcc gagcacttcg atttccgcgt agagggcgat
     4681 ttcggtgcga actcgcactc ggcggcgagc gggctgcggg tgtccgccaa cgaaatctat
     4741 gtgaactgga acaagtaccc cgcggccaat ctgcgcatcg gctacctcaa acccgcgttc
     4801 ggtgccgagc agctcgcgag cgatcctacg acgcccacga tcgagcgctt cctcgcgagc
     4861 gatcgcattg cggatggccg ccagccgggc cttggcctgt acggcagctt cctcgacaag
     4921 cggctcggct acaccgtgac gctcggcaat ggcaacggtt cgaactcgag cctcaacgac
     4981 aatgacggct tcctcggtgc cgcgcgcgtc tacggcacgc tgttcgagtc gtcccgcgcg
     5041 ggccgcctga ccggtggcat caatgccatg cgttccgacg acgcgctggt gctcaggccc
     5101 ggcttcgggt tcgacctgaa cggcagtggc ctgcccgatg ccattttccg tggcgagcgg
     5161 tccggctggg gcatcgacgc acgatggtcg ctcgggcgca ccaccctgtc cggcgaactg
     5221 ctgcgcgaga acttcaagcc ggacaatgcg acacccgctg ccgacttcga cgcccagggc
     5281 tggcatctca ccgccaccta catgctcctt ccgcagcgcc tcgaagccgt cctgcggcgg
     5341 gaggtcttcg accccaacct cgacgtggcg ggcgatgcca ccgaaaactg ggtcggcggc
     5401 ctcaactacc tgatcaaggg cagcgatatc aggctgatga tcaactacat gtttggcgat
     5461 gcacccaacc tgccggacga ttcgggccgc ctgctcgccc gcatgcagat cgtctactga
     5521 ccgctaccgg ctcaagcgcg tgaatctcct ggccctttca cctgggctcg cgggcaaggc
     5581 gagctgcggc cttcatgcga cgcgcgtgtc gctgttgccg ggcggaaagc cacagccagg
     5641 caccgctcgt gacgaagaac acgagcgaca ggcccccggt gagcacgagg actcgtcccg
     5701 gagcgcgggc cgcgcgtccg gtgtgaatcg tgtacaacca gtcgtaggcc gtattgagca
     5761 aaggcagctt cgcgggctcg tagcggccga gttcgctgcc gtcataggca ttgtagaagt
     5821 actgccgggt ggcattgggc cgcagtgacg acgggtcctg gaagtagtac gcaatgacct
     5881 ggcctttgct ccccgagaag cgcagctccc gtagcggcaa gggtccatgc tcggatcgcg
     5941 cgcgggagag cagcgtgtcg attgccatca gcggctgacc ggcctgctcc ggcacgacaa
     6001 atttcgggcg cgggaccatc ggcagcacgg attggagtgt cgcgcgatga atcagcagcg
     6061 ctcccgtcaa cccggacatg agcagcacca cggcgatcgc acccccgagt gccctgtgcg
     6121 tcgtgcgcac cgcgcgatcc gcgccctgtc ccgtgatgac gcgaaaaccg gatctgagcc
     6181 gccgacgccc cggccaccag aggatgaagc cggcaatcac cagaaaagcc agcgcaatgc
     6241 cctcgatgct gacgatgatc tctccggtcc gccccgagag cagctcgtcg tgaacgcgaa
     6301 agagcagttc cgtcggccac cgcgcatagc cgccctggcg caggatctcg ccgcgccacg
     6361 gatcgacggc gacaagacgg ggccgatcgt cttcgccacc catcacgaac agcgctgcag
     6421 cggcgccttc ggcgaaccgg atacgctgca gggtcgcgtc cggcgtggcc gcacgtaccg
     6481 tatcgacgag ttgctgaacg ggaacagccg tcgcggcggg cgtgacgacg agttccgggt
     6541 acagcaggtg ctcgatctcc tcccggaaga cgaggagagt cccggtcagt ccctgcacga
     6601 agagcaatac gccgacggtc aggccgaccc agcggtgcag cttcaagagt gcggcgcggt
     6661 cgatcatcgg cagctcattc gctggcgtag gccctgacca gccgatgcag gaacgcacgc
     6721 gcgtcgagca gcgacctcac gcggatacgc tcgtcgagcc cgtggacacc gttgccgtca
     6781 ggatcccgga acactcccga gatgccatac gtgggaatgc ctgccgcatt gagaaagcgc
     6841 ccgtccgtgg cgccgctgct gagactcggg atcaccggga cgcccggcca catgtcagcc
     6901 acgatcgcct cgacgggccg catgatccgc acctcgagcg gcggggccac gggctgaggc
     6961 ccgggctcgt cggcgagcgt gattgcgatc ttcgtgtcgg ccgcgatctc gaccagccgg
     7021 tcgcgcacgt cctcgacgct ctcgccgggc atgatgcggc agttcacgtt cgcctcggca
     7081 tgctgcggct gggcattctc ggcatggcca ccgctgatca gcgtcggaat gcatgtggtc
     7141 cgcaggaagg cgttccatag cggatccgcg gccgctatgc gtgcaagtgc ggaggcatcg
     7201 gccgttgcgc tccctgccgc caccaggtcg ctggccatct gccccacgta cagcgcggcg
     7261 gagtggcgga agtaggcttg cgtcgccgcc gtcatgtgga ctggaaagcg atacccgtcg
     7321 atgcgcgcca cggcggctgc aaggcgcccg atggcgttgt cgtccgtcgg gcgtgcactg
     7381 tgtccgccgg gcgacgtcgc ggtcagcgtg tagtcctgat agatcttctc gccgacctgg
     7441 acaccgaagg acagcggctg gccgttcgca tcgaggcggc ccgagccacc ctcgttgatc
     7501 gcaaaaccgg ccgcgagcgc ctccggctcg ttcgcgagca gatattgcac gccgttgaac
     7561 actgcgtcgg tctcctcacc gcaggtcagc gcgagtttca gcgtgcgccg cgggcgaaag
     7621 cgctcctccc ggtagcggat gaacgcgtcg acgaaggctg ccgccatggc cttgtcatcc
     7681 actgcgccgc gcgcatggaa gtacccgttc tcctcgacca gcgtgaacgg atcgcgcttc
     7741 cagtcagacg ccttcgcctc gacgacatcg atatgcgcga gcagcagaat cgcgggcagc
     7801 ctggcatccg tccccgggac gcgcgcgagg agattgccct gccgcggaaa ccccggcggc
     7861 acaatgaccc gcacatccgc ccccgggtat ccggccgcca ccaggcgcgc tgccatccgc
     7921 tcggccgcga gcgtacagct tccactcgaa aaagatgtat ccgtctcgac cagttccctg
     7981 tagagcgcga tgaagcggtt ctccccgtca cgcgcagcat cgctgtccgc ggccgcggcg
     8041 cttgcctgac cgacccacgc cagggccgcg cacaggatgg catggcggaa tactcctggc
     8101 actgcggtta ccctctttgc cgtgaccctt gccgagataa caccatgcag cttgcaggaa
     8161 gctgacgcgc cgtctgcaag cggactcacg ccacgcccgg aaagtcgatc tcgacacgca
     8221 ggccgccgcc cggcctgctt gcgatgcgca cgcttgccgc aagggcgtcc gccaactccc
     8281 tgacgatggc aaggccgagg ccgctgcctt ccacggcccg gtcgcgatcg agcctgcgaa
     8341 aacgcttgaa gacatccgcc tgcatctcct gtggaatgcc gggtccgtca tcctcgatga
     8401 cgatgcgcac cgcatcctgc aacgcgctga cccgcagcca aacggtgccg cccctgcggt
     8461 tgtagcggat ggcgttatcc atgagattcg agacgagttc gcgcgcgaac tccgccacgg
     8521 tccggacggt ggtgcggcca tcggtgtcga actggatgtc gaccccgtcg cgcaacgccg
     8581 ccggtacgta ttcctccgca accgagcgcg caagcgcggc aagatcgaca ggccgcccga
     8641 gccgggcgac gctttcccgg ttcccctccg cacgcgccag cgcgagcagc tgcgtcagca
     8701 gccgctgcaa ccgtgccgtg gcgacatcga tgtcggccag cgaggcctgc ccctccttcg
     8761 tctgcagtcc ctgccgtttc agcacctgca agtgggccct gagtatggac aggggcgtgc
     8821 gcatctggtg cgatgcatcg gtggtaaagg agcggatgcc ttccaccgcc gcatgcaggc
     8881 gcagcagcag cccattgaac gcggtcacga aactccgcag ttccaccggc acctgctgga
     8941 gcgtcagcgg tgtgaagtcg gtggccgaac ggctgtcgag gccgcggcgg acctcggcca
     9001 gcggaaagag cccccagcgc acggcaatcg gcagcagcag cacgagcagt ccgatcaaca
     9061 gcgcctcgag cgcaaacagc ccgcccagca tccgccggct caggctcttg cggccatcaa
     9121 gtgtctccgc aacctgtacg ataatcggcg cctccacgcg cggcacgcgt cgctcgatcg
     9181 tggccacgcg gatcggcgta tcgcggtagg tcgaataggc gaaccgggtc tcgtcgatcc
     9241 tcgcgttcgc gtcgccgatg cgcggcagat ccggatagcc cgtcagcagc tcgtcgccga
     9301 cgtggacgct gtaatagaca ttgtcgcgct cttcgttctc gagcatgccg aacgccgacg
     9361 gtggcaggtc gagcgtcacc tcgccatcct cgagtgcgag cgtgtcggcg atggagcgca
     9421 ccgacgcgtc aagcggccgg tcgttgacgc tctcgacgat gcggccgatg aacagcgcac
     9481 cgcccactcc cagcaccagc gcgagcgcgg cagtcggcag gatcagcgcg accagcaggc
     9541 gcgcggtgag cgaaacgcgc aggttatgcc tcaccggccg gactatcctc catgacgtac
     9601 ccgaggccgc gcatcgtgcg tatcgtgggt ccgtgtggcc ccagcttgcg gcgcaggcgg
     9661 gcgatgtaga cctcgatcgc gttcggcgcc acgatatcgt cgaagccgaa cacttcggcc
     9721 gccagccgtt ccttcgacac gaccttgccg acgcggacga cgagccgctc gagcaccacc
     9781 cattcccgcc ggcgcaaatc caccggctcg ccggcgaccc gtatcacacg ccgggaacga
     9841 tcgatttcca ggttgccgac acggatgacc ggcgacggat cggcggccga acggcgcagc
     9901 agcgcgcgca ggcgtgcact cagctccggc ggttcgaacg gcttcagcag atagtcgtcg
     9961 gcgccgaaat cgagtcccct gacccggtcg tcgagcgagt cgcgcgcggt cagcaccagg
    10021 atcggcgcgt gacagccacg cgcgcgcagg gtcttcagca catcgaagcc ggacatgccg
    10081 ggcagattga tgtcgagcat gacgacggca tagggctcgt cgcgcgccat ggccagcgcg
    10141 ctctcaccat cgtgtgccgg atcgaccgaa tagccctcgc cacgcaggat cgcaaccagc
    10201 ccgcgagcca gcgcctcgtc gtcctcgacc agcagtatcc gcgccatgtc acccactttg
    10261 acagaacagc gatcgatccg cagagtgtgg gcgtggccgg cccgatacgc aaaattccgc
    10321 gctgcggtca ccaccgcccg tccatcgccg cacgccggtg gaaattcgcg ttgctgctgt
    10381 cagccgcgac cgcggtcgcg gcggcggcgc agccacgtgg ctatccccgc tcctacgacg
    10441 gcatcatcac ggcggcgcag cgcgaaggcg tcgttgtcgt ctactcgacc accgacgaac
    10501 atgaggttgt cggcgcgctg ggcgcattcc gcgagcgcta tccgttcctg cgcgtcgact
    10561 accacgaact cgaatcaccg gacctgtatg cgcgggtgat cgcggagtca cgggcccgcc
    10621 gaccgaccgg cgacctgatc tggagctccg cgatggacct gcagatcaag ctcgtgaacg
    10681 acggctatgc gcaggcctac gcctcgccgg agaaaccctg gctgccggac tgggcgatct
    10741 ggaagaacga agcctggggc gtaacggccg agcctgtcgt catcgcctac aacaagcggc
    10801 tggtgccggc gaaggacgta ccgaccaccc atgccgcatt cgagcggctg ctgcgctccc
    10861 ggcccgagtt ctaccggaac cgcgtttcca cctacgaccc gcgcctctcc ggcttcggtt
    10921 atctgcagct cgctcaggcc tatcacgtca gccgcgacct catgccgctc actggcgcgc
    10981 tcggcgccgt cgacgtcaag ctccatacgt ccagccggcc gctgctcgcc gacatcgaga
    11041 gcgggcgcag cctgtttgcc tacaacgcgc tcggctccta tgccctggag ctcgccaccc
    11101 gcaatcccga catcggcgtc atcatgccgc gggactacac gctggtggtg tcgcgcatcg
    11161 cgctcattgc gcgcgaggca cgccagccga acgctgcgaa actgctgctc gatttcctgc
    11221 tctccgccga gggccagggc catctggcgc gcgcctacat ggccccgtcg cgcgaagacg
    11281 tcgagccgct gcccgggagc aagccgccgg ccgcggccgc acgcccggtc cgactgggtc
    11341 cggcgctgct cgccgacctc gatcgcatca ggagggagcg attcctcgcc aggtggcagg
    11401 cagcgctcga ggcgcgatag gcgcagttca cgcatgccat ggaaacaggc cgaacagcag
    11461 gcagtccagc atgaacacgg cgcacgcgcc cagcgcccac ttcatcgtga agcgctggtg
    11521 atctccaagc tcgacgcccg cggtcgccac cagcaggtag gtcgaagcga ccagcggact
    11581 caggagatgg atctgctggc caacgagcga ggcacgcgcg atctccatcg tcgagatgcc
    11641 gtagtgctcg gccgactgcg ccagcaccgg cagcatgccg aaatagaaag cgtcgttcga
    11701 gatcagcaca gtgaacggaa tgctgacgag cgcggtgatc gacgccatat agggaccaag
    11761 cgcgtccggc acgacgccgg tgacggtatt cgccatcgca tcgaccattt tcgtgcccga
    11821 cagcacgccg gtaaaaatcc cggcggcgaa caccagcccg gccgtcgcca gggcattcga
    11881 cgcgtgcgcg gccacgcgct cccgctgcat ctcgacctgc ggataattga ccattgccgc
    11941 aatggcgaat gccaacatga acagtacctg cagcggcacg tccccgaaca gcaggatccc
    12001 catcagcaat gcggtcagcg ccgcgttgaa ccagaacagc cgcgggcggc gcgtggacgg
    12061 cgtgccgtcg ctgtcaggca tcgacaggtc gagttcgccg ggtgacggca tcgccatacg
    12121 ctccgtgata ccgaggcgct tgcgctccgc gcggcccagc acgtatgccg tgaacagaac
    12181 ccagccgacg gccgccagca ccggcgggat cagcgacagg aacagttcgc ggggatcgac
    12241 accgagtgca atcgccgcgc gcgttgtcgg accgccccac ggcaccagat tcatcgccgc
    12301 aactgccatg atcagtatgc aggtgagcac ccggatatcc atcttcatgt gcttgtacag
    12361 cggcagcagc gccgagagcg tgatcatgta ggtcgtagtg ccgtcgccat cgagggcgac
    12421 aaccaggccc agaatcgccg agcccatcgt gatgcgcagc ggatcgccat gcgcgagctt
    12481 cacgaccctg ctggtcagcg gatcgaacag acccgcatcg atcataatgc cgaagtagag
    12541 gatcgcgaac agcagcatca cgcccgtcgg cgcaatatcg cggatgccgg cgagcatcat
    12601 gtcgccaagc ccgctccaga agcccgcgaa cagcgcgaat acggccggca cgatgatcag
    12661 cgcgatcatc gccgacagtc gcttcgtcat gatcagcgtc atgaaggtgg cgaccatgcc
    12721 gaaggcgaga attgaaagca tcacgcagcg accccccgtg cacccgcgcg aacgggagca
    12781 ttcctgtcac tgactccgat tctggaggtc gtgtctttca tgaagctgtc agtgacaggc
    12841 gccggagcat gagcggcaac aacccgcccg cacgcagcgc cgccacttcc gcgagcgtct
    12901 ccaccgcagc gcgcgccgtg aagcgatgcg tgcgcccgtc ctgatagcgt gtgccgacaa
    12961 cgcaaggcgc cgaggggacc agcgcatctg ccggcatatc gatctcgatg cggtcacccg
    13021 gcgccagcgc aagcgaaccg ggactcattc cggcaggcag ttcgatgggc aggatgccca
    13081 tgtgacacag gttcgatctg tgtatgcgct cgaagctcgc ggccagcacg gcgcgcacac
    13141 cgagcaggcg caggcccttc gccgcccagt cgcgcgagga acccgtgcca tagcgctcgc
    13201 cggcaatgat caccactggc acccctgagt ccgcgtagcg ctgcgcagcg cgccacaggg
    13261 ccaggatttc acccgagggc acgtgaaccg tcgcgccggg cggaatatcg gcgcgcagca
    13321 gattgcgcac cgatacgttg gtgtagagac cgcgcagcat cacttgccaa ttgccccgtc
    13381 gggcagcgaa tacattgagg tcgtccggat tcgcgccgtg ttcgatcagc cagcggcctg
    13441 catcgccact cgccgcaatg gcaccggcag gcgagatatg gtccgtcgtg acgtcatctc
    13501 cgagcaccag cagcggctga gcaaggtaac ggccaagctg cgtgcgtgca tccggatcga
    13561 cgaacggcgg cagccgcagg taggtggaat tccgatccca ggggtagagc tcgccccgcg
    13621 gtgcagccaa tgccgaccag gccggactcg cctcggccgc gtcgtatgca ggaccgaaat
    13681 cggcgggatc ggtcgccagc gccagcacgg cgtcgatttc ggcgtcgtcc ggccagatat
    13741 cgtgcaggtg gaccggagtc ccgtccggcg agtgtcccag cggatcggtt tcgatgttgc
    13801 gatcgaagtc gcccgcgagc gcataggcaa cgaccagcgg cggcgaggcg aggtaggaat
    13861 cctcgatccg gggatgtact cgacccggga agttccggtt gcccgacagc accgcaaccg
    13921 ggcgcacccc ggaccccaga cgttcgatgc cgggtgccag cggcccgctg ttgccgatgc
    13981 aggtcgtgca cccatacgcg acgatggcga agcccaacgc ttcgagcggc gccagcagac
    14041 ctgcgcgctc cagcatcagc ctcgcggtcg gtgagccggg cgcgagactg gtcttgaccc
    14101 aggactgcac cgacaggcca cacaccacgg cgcgtcgcgc caggagcccc gctgcgacca
    14161 acaaccgcgg gtcggaggtg ttggtgcagc tggtgatcgc ggcgaccgca accgggcgca
    14221 tgccaccgcc cgtcgccgcc gtggcccgcc tgtcgatccg gtcctgcgga cggcgcggcc
    14281 cggcgatacc gggcgcgacg gccgcaagat cgagatcgat gacacactcg tagtcgggct
    14341 gcgcatcggc atcgaaccac aggcgctggc agcgcgcgta gcgctccacg cgttcgatat
    14401 ggctgtgctc gcgccccgtc gtgcgcagat aagtcagcac ctgctcgtcg atcgggaaat
    14461 agccggtggc gccaccgaac tccggcgcca tgttcgcaac caccgcgcgc gtccccgcgg
    14521 agagcgtcgc gacgccggag ccgaagaact cgacgaagcg cccatcgacg cagacatcgc
    14581 gcagcaggtg ggttacctcg agcgcgagat ccgtcgccat caccccggaa cgcagcgtgc
    14641 cggacaggcg cacgccgacg acctccggca cccgcagcga aacgggcacg cccagcatca
    14701 cgctctcggc ctcgagcccg ccgacgcccc agccgagcac accgataccg cctatcatcg
    14761 gcgtgtggct gtccgtgccg atcagcgtat cggggaaaat ccactcgcgt ccctgccacg
    14821 tcgcgacttc cacgaccgaa gcgaggcgct cgagattgag cgtatgcatg atgcccgaac
    14881 cgggcggatg gacgcgcagc gtgtcgaacg cctgcgcggc ccatttcatc agactgaaac
    14941 gctccgcgtt gcgctgcatc tcgatggcca tgttggccgc cagcgcacct ggtacgccgt
    15001 agcggtccac cgaaatgccg tggtcggtcg atacatcgac acgcagcccg ggcctgacgc
    15061 gcagcggatc tcccccacgt gcggcgagca ccgtgcgcat cgcggccagg tccacgaggg
    15121 cgggaccgca ggtggtgtcg tgcatcatca gacgcgacgg cgcgaacggc agttccgtgg
    15181 cgcaatcccc ctgctcgagc cagcgcccgg ccgcacggtc ggcatcggcg atcgcatggc
    15241 ggtgacgcaa cacgttctcg gcgaggatgc gatgcaccca cggcatgcga tcgagccgtg
    15301 ctcccagcag ccccggcaga tccaccctcg tcggcccggg cccggaggag cccgtgccgg
    15361 gcgccgcgtt catgcgggcc cgtccgtcgc agaggcgcgc agatccgccg gcccgtgctc
    15421 ttcatcgatg cgcgtcgtca cgccggtcgc gacgatggtt cgtgtcgcat cgacagaagc
    15481 atatgtccag aaaatctcga accgctcgtc ggcggaagtg ttgcggaaga aatgcgcaat
    15541 gccggccgga atccagctga cgtcgcccgg gccgacatcg tgcgccttgc cgtcgacgtg
    15601 ggcgcggcca tggcccgaca gcacgaggac gctctcctcg caattgtgga aatgcagcgg
    15661 cacggcagca ccgggcgcga gcaccgtaat gccgttcagc atctgccgtg acccgacccg
    15721 cgcggtcacc atcggcgtcg tgagtatgcc cccgccacga tcggtcgcgg gccgctgcgc
    15781 gacgcgcagc accacggggc gacccggcgt cagcaccacg ggctaacctg cgcggacggc
    15841 accgcagacc acctcaccga aggcagacgt gccgagcgtg ccaccgatgt cgcgagtgcg
    15901 agagccgggg tcggcgagca cccgctccag cgcgccctcc agtactcgcg ccgcgcgcag
    15961 cagatcgctg cgaccgctgc gacgcccgat ccagtcgagc agcatggcgc aggacagcag
    16021 cagcgatgag ggattcgcaa tgtcccgccc cgcgatatcc ggcgccgatc cgtgctgcgc
    16081 ctgtgcaaca cagatgtcat cgcccgcgtt gatcgatccg ccgaggccca ggctaccgct
    16141 caactccgaa gcttcatccg aaagtatgtc gccgaacatg ttggtcgtga ggatgacgtc
    16201 gaaccgatcc ggattgcgga ccagcagcgc cgcggtggca tcgacaatca gctcctcgat
    16261 ctcgaccccg gggaattgcg tcgcgacctt gcgcacctcg cgcaggaaca gaccgtcggt
    16321 cagcttcagt acgtttgcct tgtgaacggc ggccagcttc ccgcggcgct cgcgcgccag
    16381 ctcgaaggcg atgcgcgcga cccgcgatac accctgcgcg gtgattttgc gcacggacag
    16441 cgccatgtcc tgatcgggca tgaactcgcc attgccggca tacatgttgc ggtcggagta
    16501 gaagccctcg gtgttctcgc gcacgatcac gagatccatc ggccgccgca gcatcgtcag
    16561 cccgggacgc gagcggcacg gccggatatt ggcgtagagg tcgaactgct tgcgcaatgc
    16621 gcccgatgga ttgattccgc cggcctcgcg cggcgggtag tcggcatgcg agaccggacc
    16681 aaggatcaca ccgtcgactt cgggaatccg cgcgagcaca aggtccggca gcgtggtgcc
    16741 acattcggca aggctcgcaa gaccgactgc acgttcctcg atcgcgatcg cgagagcgca
    16801 tgtgtcggca agcgcctcca gcacgcgccg cgtgacggcg gcgatctccg ggccgatccc
    16861 gtcaccgggc agcaggagca gcttcatgcg tgatccggat tcccggccca ctcgaccggc
    16921 cctgcccttc gtcggggccg gccggtcgat gggcccgcat ggtgcatccg ctcagaacat
    16981 atccgcggtc aacgatagct tcaccgtcga cggcgttccg aacgcgaagt agttggtgct
    17041 ggtcgaggcg aaataacgct cgttggtgat gttctgggcg ttgacgcgca cggtcatgcc
    17101 gactccgccg acctggaagc ggtacgagcc accgagatcg tagagcgtgt agctcggcac
    17161 gaacagcgag ttctgcggat tgatggcccg atcacccgtg taatagacgc ccgcgttgat
    17221 cgcgaggccg tcgatgaacc gcgtcaggcg gtactccgcc gcgactgacc actgcgtcct
    17281 ggccgtgttc tcgatccgct tgccgatcag cgccgcattg gcggtctcgc cctgtttcgc
    17341 gcgcagcgac agccccgagg catataccga cagatcgggc gtgatctcgc cggtgacgct
    17401 gaactcgaag ccgcgatagg tcgcctcgcc atccttgacg aaaaagttcg cggcattggt
    17461 gtaggtcagt tcgcgcgtga tgtcgaagta cgcggccgtg aacaacagcc ccttcctcgg
    17521 ctcgagcttg acaccggcct cgtactgcgt ggaatcactg gccggaagaa tctcgccggc
    17581 gttgttggcc gccagtggcg cggccggcgt cgattcgagc ccctcgatgt aggtgccgta
    17641 gatgctcgcc cactcgaccg gcttgtagac gatgccgccc gacagggata ccgggtcgtc
    17701 ggagaaggtt tcgatggccg ggccagtggc ggttctctgc tcttccttgt agtccgagag
    17761 gcgcacgccg gcgaggatgc tgaacatgtc gtcattcggc ccgccaaagc gcatccggtc
    17821 gaacgcatag aaacccgtgt cctcgatcga cgtgtcgcgc gccgcgttat aggtcgccgt
    17881 accgtcgaaa cggatgtcgg cgaggtcgac cggatcgtac aggctctgca ggcaggtggt
    17941 cgtgaggccc aacagtacac agttgttacg tgtcgcagcc gagccggttc ccactctggc
    18001 gacggccggt gccgagtact gccgccggat gttcttcgag gcgccgacca gcagctcgtg
    18061 gacgacctga cccgtcgcaa gggcgccggc gagttcgatg cggccattcc tgttgcgata
    18121 gagctgcccg tccgccgcac tcattgtgag cgtaccgaga cccgtgaccg gattgaaatt
    18181 gccgagcgag ctgaagcggc ggtcacgctc tgcataggaa agtccgccct cgacggtcag
    18241 atcccaggcc gagttgatct tccacgaaag ccgcccgagg acgttgtcct cctcggcgcg
    18301 attcatgaaa ccttcgctcc cggggttgtt cgacgctcgc ggcagtctcg gcagcacggt
    18361 cagcaggttc gcggccgagg taggtccctg cacgaccgtg ggctcggtca tttctttttc
    18421 gatgtgctcg acatccagat tgaagcggag gctgtccgtc agttcgtact gcagcgcacc
    18481 ggacaggaag gtgcgatcgc cgctcacatt gtcgatgccg ggatcgattt caccatggac
    18541 gaaattgccg cgcagcccga gcgcaccgat cgtgccgctc gcgtccaggt gaccctggat
    18601 ctgcccgaag tcgttgccat tgaccgcgac gctcagcatc ggatcgaccg ttggtcgctt
    18661 ggtcgtgagg ttgatgattc ccgacggcgt cgtgaagcca tagtaaagag cggaggcgcc
    18721 cttcagcgcc tcgactcgca ccttgttctc gagcggcagg tcgatcaggt tgacgatcgg
    18781 cagcgaaccg ttgagacggt agttgccacg gttctcgacc gggatgccgc ggatcgagag
    18841 attgttgtag accgtcgggc tcgtctgcga actggtgacg ccgggcgagt tgcgcagcgc
    18901 atcgagaatg ctctgcgcct gctgcgactt cagcacctgt tcggaaatga cgctgacggt
    18961 caaaggcgta tcgagctgac gcgcgccgcg aaagctcccg gcctgcacga agtcggcgcc
    19021 gtaactgtct gggcgatccg acgtgacgac gatttcctcg agcaccggtc ccgagcgggc
    19081 atcctcggcg gccggctcct gtgcgtcggc ctggctggcc gccaccattg cgaagccgcc
    19141 cagtccgagc gccgcgatcc gattccagag tatcgacatg atccactccc ccgtttcgaa
    19201 acttccgccg ccttgcggcg cggcaggccg aacatatgcc cgttttgctg tcacaaacct
    19261 gtcccagccg tgacatcctg catcgctcgc gcagccgcaa taggccgggc ctcgccagct
    19321 ccccctcctg tgacagcgtg acgacagcaa tccttcctac ggttcgccca gggacccggc
    19381 caacgaccct cacggagcct gacaatgacc gcagcagatc cccaccggat ttcccgccgc
    19441 cgcttcacgg gcatgacact cggcggtacg gccggactgc tgctgccgaa tctcgccgcc
    19501 tgtcggtcgc ccgccgtgcc tgccgcatcc gcggcagacg gtctgccgcg aaaccggcct
    19561 gaagcgcagg gtgtcgaccc gcggggcatc cttgccttcc tggacgaggt ccgggcggag
    19621 gggctcgaac ttcacagctt catgctcgcg cgcggtggcg acgtgatcgc ggaaggctgg
    19681 tggtccccct atcggccgga cctgccgcac atgatgcact cgctgacgaa gagcgtggcc
    19741 gccaccggcg tcgcactggc cttgcaggag gggctgttcg ggatcgacga caaagtcatt
    19801 tccttcttcc cggacgaact tcccgcggag gtcagcgaca atctcgcggc gatgacagtg
    19861 cgcgatctgc tgacgatgcg cgccggacac gccgaagaga tttctggctc ggtgtggcgc
    19921 cagatcagga ccagctgggt aaccgaattc ttcaaggttc cggtaccgct gaagcccggc
    19981 acgaaattcg tctattcgag cgcggtcagc ttcatgctct ccgccatcgt caccaagctc
    20041 accggccaga cgctgcgcga ctacatggag ccgcgtttct tcaagccgct cggcatcacc
    20101 ggcctcagct gggacgtcgg ccccggcggc atcaatccgg gcggcaacgg cctcacctgg
    20161 aaaacagccg acgcgctaaa gctcggcatg ctccatgcgc agaacggccg atggaacggg
    20221 gtgcagatcc tgtcgccgga atgggtgcgc gaagccagcc ggccgcaggt cgcggaaggt
    20281 gagtatggtt atcagtggtg gatcgggccg aacggcgcct actacgcact gggactgttc
    20341 acccagatgt cgatcgtatt cccgcagcat gacgccgtgc tcgcggtgac cgccgcgatt
    20401 gacggcagcg aacacctggc gcagatcgtg tggaagcatt tcccgcaggc cttcggcgca
    20461 ggaagccggc cgcgcgacga tgcggcccac gccacgctca cgcgccgcac cgccagcctg
    20521 tcggtgcagc cggagctgtc ggccggcagc tcgccgaccg cgtcccgcat atccggccag
    20581 cgatttcgcg cagcagccaa tgcggagggc atcgaggatt tccgcttcac gttcgacggc
    20641 gatcgctgcc ggttcaccgt gcgtgacggc cgcggtgaac acaacgtcga cgtcggcgtc
    20701 ggccgctgga tcgagggccg cacgagcatg accggcaaca agctgcacca tcagtaccag
    20761 ccggattcga tgttcgtcgt cgccggcggc caatggaccg cacccgacac cttcgagatg
    20821 acctggcagt tcgcggaaac ggcgttccgc gacaccgtcg tctgccgctt catggacgat
    20881 cgcatgactt tcgaccgcag cgtcaacgtc aattcggcgg cccgttcgct gccgacggtg
    20941 caggccactg tgctcggggc tgcagagtga tgcccgggcg ctgaccgcat gcggcagctt
    21001 gtgtgtgcct gtaccgggct cgtgctactg gcggcgaccg ccgccctcgc cgagtctgca
    21061 ccggccgaac gcttccccgc gcccgactgc acggccccgg tagcgtacga ccgcgacatg
    21121 accctgccag gttatctggt ggacaccgac cacggcacca gggtttgcgt accctttacc
    21181 accgttggcg cccgacctcc gcagggctac agcggcgatt tctatgtcga tgagttctcg
    21241 gacgaggcgc tgcgtgcgcg ctggctcgaa tgccggacgg atgccgcctg ctcggcgcgt
    21301 gtgacccggg tcgtggagcg tcgcctgccg cccaaccgcg agtctcgcat cacggacccg
    21361 caccatcgat atctgctcgg caagatcgcc gaagcaccga acctcgatct gcgtagcatc
    21421 cgccggccgg gattcttcgc acgtgcgccc tatcgggagc cgatcgctgc cgcagaacgt
    21481 gtcacgagca cggtcgagtt caccgcacct gcggaagcct acgaacgcgc ccacgggatc
    21541 gatcagccga tcaagctgcg tggctggtac ctgcgcggcc gcggcgtcct tgatgcgatg
    21601 ggcaaacgcc gccgcgcgct gctagtgatg agcggtggcg gcggcacgcg gctcgtggcc
    21661 atcgaagatc cttcggaccg gctctacagc gtggatccgg ccaccggcaa gacctcgatc
    21721 aacgcctttc ccaatgccac gacgggatcg ccggggcagg ctggctggcg cgcgatcatg
    21781 gcgatgttca acgaggccgg cttcgacgtg ctcgcctacg atcggcgcgg cgtcggcgta
    21841 tcgagcggct acagcgatac caatacgctg cagcagggac gggatctgct cgaggcgctc
    21901 gccagtctgc gctcgggcga cggcttgcgc gtgctgacgc cgaagggcac cgttctgaac
    21961 ggcgccgcgg ctgcagaggc gctgcgcggc ggtacgggcg aactgccgac actgctgctc
    22021 ggcaactcgc ggggcacgat ggccagcggc tgggccatga cgatcaactt ccaccgggat
    22081 tgcagttacg accagcccga gatcgtgtgc gcgccaccgc gccgcgatga atcgatcaaa
    22141 ggcgcgctgc tgatggcgga gttcacgagc ggccccggtt tcgtcccggc gacgcccaca
    22201 cgcgaggacg aagggcgcgg cctcggcaag gatcgcggcc tgttcatcgg cgcaagcgag
    22261 gtcgagcacc agatcatttt tttcccggga tccgcgatcc tcgccggcat gcgcgcatgg
    22321 ccggccgcgt tcttcgtgcg cgggctctgg gactatgccg cgagcctgga aggcacgatc
    22381 gcttcctatg atcgcgtgcc cggcctcaag gaactcgtgg tcgtgcgcgg cccgcaccct
    22441 tacgaaacct ggccggaggt cgagaaggag cgcgtccgcg cccgcatgct ggccttcgcc
    22501 cgcgcagtga tacgcggcga cggcgaagtg cccggcgcgc gcaaatggac caccatgaga
    22561 gagctggtcg agacgacgga agacgtgtgg gaacgctctt cggccccgcg agcaccctga
    22621 aggcaacggc tagggtgccg cgccaagcca gcggtcgaac cacgcgacga tgcgctgcag
    22681 tgcatcgtca cgctccggcg tggcgccttt catgaagaca tggccgcccg gcgcattgtc
    22741 gtagatcttc gcttcgtaag tcttgccgtt cgccttcagc gcatcgatca ggcgcccggt
    22801 gtgcagcgta tgcggcacga tcttgtcgcc gctcgtcgcg agcaccagca ccggtgcctc
    22861 gatccggtcg acgaaattga tcggtgacac gtcgatatag gcgggcagat tctcgtgcgg
    22921 cagcttgccc ttgaaaccgg gattggtctc ggcgatttcc ttgcgccggt agtcgggctt
    22981 gtacgacatg taggccacga agtcggtgag cccgacgatg tccaccgcgg ccctgaactg
    23041 cttcggcgcc cgttcgatcg cgagcagcgt caccatgccg ccgcggctct ggccgagaat
    23101 gccgatgcgc gcgggatcga tgtcggggcg gccggctgcg taacgaccgg cggcgagcac
    23161 gtccgccacg tcggtgctgc cgtaatcgtt ctggtagatg gcgtcaccgt agccgcggct
    23221 gccgcgatac tccgggaaca gcacgacata gcccgcctcg acgaccgcct cgatcagcgg
    23281 aaaccacgac ggctcgagcc gttcatggaa gccgccgtgc accatcacca cggcgggacg
    23341 ccgtgtgccg gcatcctcct tcaacggcgt gaagacatgg gtcgggatca gctcgccatc
    23401 actgttcacg aacacggtcg agagccggat ccggtccgca tgatccagcc ggaactgcgt
    23461 caggatcgcg aattgctggg tgacgtagat ttcctgctgc agatgcttca gcagcgccgc
    23521 ggcatcgtcg gtcccggggt tggtgaagct gtcgagcgcc ccctccctcg tgtcggcgct
    23581 gcgcgagaaa ctgctggccg cgccgcgccc gtcctgcgcc gatgtggtat cgggcgcggc
    23641 agcgagcagc gcgagcattg cggtcgctat cagcatgacc cacgatattg ccggaaagct
    23701 gtcgccatgc tgtcgtattt tccgcggatg gacaacctca cgctcgccag gatcatcgcc
    23761 gcaatcggca gccggacttc ccggccgtgg cggcggaggc catgcgcacg ctgatcggct
    23821 tcgacctgac cgccgcgcgc agaacgtcgc cgcggacgat gaagagctcg gctttcgcac
    23881 cctcggctgg cccgcaaacc aggaggaact cgacctctac gtcgatgccg atgggagtct
    23941 cgcggccgcc atcaccccgg taacgcgact gatcagcgtc acctgcccgc acaatcccac
    24001 cggcacgatg ttcactgcgg tggtcgatga atggattgcc acgcaggtcc tgtcccgccg
    24061 cgagccactg cttcgacaga cgctggggga gatgcgcccg cggctcgatc tggtcagcga
    24121 tcggtccggg ccactggttc gagctgccgg atacctgctt ccggctcgac tacggttggc
    24181 ccgacgccga gcgcctcgaa gcggggctcg cgggcatctc gcgcgcgctc agggactgac
    24241 gggaacgtcg gatctcatcg ccgccggcat cgcgggcccg cgcgccctgg ccctgcgccg
    24301 cggccgcctg cgcgtacttc ggcccttcgc gcaatgcttt gccgaccatc gagacgagca
    24361 gatcgagttc gtcggcggcg agcgcgaagt gcggcgtgaa gcgcagcgaa ttggcgccgc
    24421 cgtggatcac gccgatgccg cgctcgcgca gccactcctc ggtggagccg gcgccgtagc
    24481 atttgtactg cggcgcaagt tcgcacgaga agagcagccc tgtgccctgc accttggtga
    24541 tcgcaccgcc gagttcggcc ttcagttgtt cgagtttgcg gatggcctcg cggccgcggt
    24601 cgcggatatt ggcgcgcagc tccggcgtta acgcaccgag caccgcgcag gcgacgtcga
    24661 gcgcgcgcgg gttggtcgtc atcgtgttgc cgtagatgcc cgcgcgatag agcctcgaga
    24721 cctgttcgct caccgccagc accgacagcg ggtactggcc cgcattgagc gccttggacc
    24781 aggtctccat gtccggcgcg tcgaggcctt cgaagccggg gtaatcgacg atcgacagca
    24841 cgccatgcgc gcgcaatccg gcctggatcg aatcgaccag cagcagcgaa ccgtgcgcac
    24901 gagtgagctc gcgcgcggcg gc
//
LOCUS       DAOQVY010000026        23773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_2494_length_23773_cov_5.610296, whole genome shotgun sequence.
ACCESSION   DAOQVY010000026 DAOQVY010000000
VERSION     DAOQVY010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 23773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23773
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2494_length_23773_cov_5.610296"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..602
                     /locus_tag="PKN91_04110"
     CDS             84..602
                     /locus_tag="PKN91_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNS27288.1"
                     /translation="MQSPVNGIGQLRRNSVHRLQIGHAGVCHTAHPAKALEQFGAPLG
                     AHTGNVFQATAAGARSATLVHCDLHGSNLIEGDRLWLIDWEYAALADPLQDVASVLAY
                     YPHAGRHSGRLLGALGLAGVTPRELLAAVWMFQLLVFLWYRVRRLAVAPTSADLAAER
                     RAHAALVHNGNL"
     gene            618..932
                     /locus_tag="PKN91_04115"
     CDS             618..932
                     /locus_tag="PKN91_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNS27289.1"
                     /translation="MSVLKVVDRDGVEHEVEAQPGIKVMEVLRELDYGVAAICGGMCS
                     CATCHIYVDPEWVGRLPAAMSDEKDLLSELSHVQENSRLSCQVDFTAALDGLKVVIAP
                     DE"
     gene            943..1722
                     /locus_tag="PKN91_04120"
     CDS             943..1722
                     /locus_tag="PKN91_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNS27290.1"
                     /translation="MTDTVLVEIRGDVAVLTLNRPESANTLDLDMGRDLLAAALRCSS
                     DPAVRAVVLTGAGRNFCFGGDLRGMVSGGAPVEPYLRDLTSHLHAAISQFMRMDAPVV
                     AAVNGTAAGAGVGLVAMADLAIAGEGSRISLAYTGVGLTPDGGTSFLLPRAVGAKRAS
                     ELILTNRALSAAEALEWGLVNQVVADAEVFDAALQLAGKLAAGPRAAFGKAKRLLGAS
                     LGALEQQMALESETIAIQAATAEGGEGIAAFLEKRKPQYPR"
     gene            1728..2744
                     /locus_tag="PKN91_04125"
     CDS             1728..2744
                     /locus_tag="PKN91_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HNS27291.1"
                     /translation="MDNRQWILVKRPFGEVDDQTLALRTAPVRELADGECLIRNLYLS
                     LDPTNRIWMSDQEQYLPPVAIGEVMRGLTIGRVVASRNPRIKVGDPVQGVGGWQDYWI
                     APDGAAAKLRVEGDTPLTLYMGLLGMVGCTAYFGMLEIGNPQPGETVVVSAAAGAVGS
                     IAVQLAKLRGARVIGIAGSDEKCGWLIDELGIDAAINYRREKVRTRLRELAPKGVDVY
                     FDNTGGEILDAVLANLALNARIPLCGLISTYNATGRGSGPANYGAILIKRAMVKGFII
                     LDYQARYGEAIRALADWYAQGRLKYRVDVVDGLESAPRALGKLFDGSNQGKLVVRVAA
                     DTAH"
     gene            2811..3233
                     /locus_tag="PKN91_04130"
     CDS             2811..3233
                     /locus_tag="PKN91_04130"
                     /inference="COORDINATES: protein motif:HMM:NF018574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HNS27292.1"
                     /translation="MTLAYIFLFFGGAIAIWRIWINIKRARDRPAHSWDERLIEKLRL
                     EGSDPFQPHDVVFFFGLPSEKSAQLIVERLVREGYQAEYRQVREQVELAFSVHAQRSL
                     RLSVPDMQATSRRFNELAAEFHGRYDGWAAASNRRIGD"
     gene            complement(3237..3986)
                     /gene="gpmA"
                     /locus_tag="PKN91_04135"
     CDS             complement(3237..3986)
                     /gene="gpmA"
                     /locus_tag="PKN91_04135"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNS27293.1"
                     /translation="MPGKLLLVRHGQSTWNLENLFTGWTDVDLTKQGRAEAHQAGVEV
                     KREGLQIDIAFTSVLKRAIRTLWYILDETDRMWVPVERSWRLNERHYGALQGLNKAQT
                     VKRHGEAQVKLWRRSYDVPPPPLASDDRRHPRFDPRYAGIDPALLPATESLKDTLARV
                     LPYWNERIAPELRAGRNVAVVAHGNSLRAMVKMLDDMSEADIVELNIPTGVPLLYELD
                     DALRPKSSRYLGDAAAIAAAAEAVKRQTAAK"
     gene            complement(4029..4853)
                     /locus_tag="PKN91_04140"
     CDS             complement(4029..4853)
                     /locus_tag="PKN91_04140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS27294.1"
                     /translation="MRLSLVVTTYERPDALAAVLATAAAQRQWPAEIIVADDGSGPAT
                     REVVAAFAAQAPAPVAHVRQDHDGFRAARLRNLAIAAARGDYLVFVDGDMLLHPEFIA
                     DHRRAARPGCFVQGVRIPLDAEATKAALAAARDAPGAVPRHARGLRRIWALHSPALAR
                     CSARIGNAVVATKSCNLAAWRDDLLAVNGFNHAMVGWGPEDKELCARLGHLGRHRRAL
                     VFGGIAWHLHHPPATRDRRARNEAILAATRRERTVRCEHGLAEVAQSTMQDGEMCL"
     gene            complement(4853..9682)
                     /locus_tag="PKN91_04145"
     CDS             complement(4853..9682)
                     /locus_tag="PKN91_04145"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HNS27295.1"
                     /translation="MTRIVPAARLALIERIVKAAPARGSSRQRTADAQFLRAYFRGVS
                     EDDLAARPPADLAGVGLDHLALGRTLAGAQARVRVFNPDAARDGFASPHTIVQIVTKD
                     MPFLVDSIGIVLAQAQVAMHFIVHPVIPMRRDARGRFLGVADSDDPQSSLESWQLYEI
                     DRQSDPAALARLEESIRATIVDVQAAVDDWMPMRDSVRQLAAGLADATPLPADEITEA
                     RALLEWMEAQHFVFLGYRRYRLVRGASEDRLVPAGRSGLGILRQGHRGRRSRTVRLRG
                     ELRQRAREPELLILTKANTLATVHRGTYLDYVGVKTFDAQGRVDGEHRILGLWTSTVY
                     YRSARAIPVLRRKVEQVIDHFGLAPQSHDAKAVVNVLETYPRDELFQANVADLIRIVR
                     EVVNLYDRRTVRLLARRDPYGRFYSCLVYVPRDRYSTDVRHQIERILLAGFKGVAFES
                     QIQISDSRHARVHVVVRVDRKQRTNVDVARMEREIAAAATNWMDRLSAALKAQHDESA
                     ALALMSRYARAFPTAYEEDVAASDMLEDIADLESLRGSEPGALRLNLHRPPGQDAKRV
                     NLKFVKLGETIPISDMLPLLENFGLRVITERPYELSWPDGGEAWIQDFELEHRDRIEL
                     DIDRVEPLFNEGVAAAWRGDIENDGFNRLVLLADMPSRDVVVMRMIAKYLVQTAVPFS
                     QASMERTLAHHAGIARQLIALFHARFDPAAPERARARNVERIVAGIGRALTQVTSLDD
                     DRILRGYLAVIKAMLRTNFYQRDAAGRSKDYVSMKLDPARIPDLPLPRPRYEIFVYSP
                     RVEGVHLRMAAVARGGIRWSDRREDFRTEVLGLMKAQNVKNTVIVPAGAKGGFVPKQL
                     PVGATRDEIQKEAIACYQTYIRGLLDITDNIVGTEVVPPRDVVRHDGDDSYLVVAADK
                     GTATFSDIANSISVDYGFWLGDAFASGGSAGYDHKKMGITARGAWECVKRHLREIGID
                     SQSQDFTVAGIGDMAGDVFGNGMLLSKHIRLVAAFNHLHIFIDPQPDAARSYLERQRL
                     ARLPRSSWDDYDRKLISRGGGVFPRSAKSIALSAEAQRALGVSVSSATPQEVIRAVLR
                     MPVDLLWNGGIGTYVKAEAERNAEVGDRSNDAVRVNGRELRARVVGEGGNLGCTQRGR
                     IEYAANGGRINTDFIDNSAGVNTSDVEVNFKILLADPVRSGRLSLAARNRLLRGMTPD
                     ISALVLRTNYLQSQSISTLELNTVARLSEYRGLIRSLERAGDLDRNIEFLPSEDEFIE
                     RAKRGAGLTRPELAVLLAYSKIWLSGELIDSDVPEDPYLSEELHRYFPEALRRKFARD
                     IDRHRLRREIIVTATTNSLVNRMGPVFAWRAVEETGATIGQIARAYTIARESFEMRAI
                     WSEIEALDSRVPAQAQYLAYDYTARLLRHMSYWLLQYRRRNLHVERTVAEYRPGVREL
                     GDALHGVTTGLVPEHNAARRAQLVSAGLPEDLAARIGALESMEPVYDLVELAAEHRKS
                     VRDAARIYYAIGAAVGLDWLRTQIGALAASGPWQANARSTLRNSAARAHRALAGDVLR
                     SMRRRGAARDPVAAWVQSQGDEFARWSRMLADMRAADHPDFATLSVGVEAIRKLASA"
     gene            complement(9694..10179)
                     /locus_tag="PKN91_04150"
     CDS             complement(9694..10179)
                     /locus_tag="PKN91_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNS27296.1"
                     /translation="MPPTPSLQLDRFVPYRLSVLANLVSSAISGAYAERYSLSIPEWR
                     VIAALGEQPGMSAADVAARTAMDKVAVSRAVAALLRARRIVRTQSRRDRRRSVLRLSR
                     TGAALYARVVPQVLEYEAALLGVLGRDERRALDRLLEKLTRRAGELTPCIIARLAPNA
                     R"
     gene            10248..11294
                     /gene="hppD"
                     /locus_tag="PKN91_04155"
     CDS             10248..11294
                     /gene="hppD"
                     /locus_tag="PKN91_04155"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HNS27297.1"
                     /translation="MAAIHDNPMGTDGFEFVEYTAPDPELLRSLFARMGFPVTARHRS
                     KDVTLHRQGDVNFLINAEPDSFGQQFARDHGPSVCAMAFRVADAARAFERALSLGARP
                     HQGRIGPMELNIPAIEGIGGSLIYLVDRYGDKSIYDVDFRPVGDTGDGVGFTRIDHLT
                     HNVHRGGMDRWAGFYERLFNFREIRYFDIEGRHTGLFSRAMTSPCGKIRIPINESQDD
                     KSQIEEYLRLYHGEGIQHIALATDDICRTVDALAARGIVFQDTPDTYYEGIDARVPHH
                     GESVEELRRRRILIDGNPDRGEGTLLQIFTQNVIGPIFFEVIQRKGNDGFGEGNFRAL
                     FESIELDQVRRGVI"
     gene            complement(11313..12404)
                     /gene="zapE"
                     /locus_tag="PKN91_04160"
     CDS             complement(11313..12404)
                     /gene="zapE"
                     /locus_tag="PKN91_04160"
                     /inference="COORDINATES: protein motif:HMM:NF015902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HNS27298.1"
                     /translation="MPTAVLARELGRRGLVPDPAQLAAIDALEDLRRRLVAAERAGRN
                     PLARVRGLLRPPERRAGRGIYLWGGVGRGKTLLMDAFHASLPFEARRRRHFHRFMHEV
                     HAQLAALAQTESPLTVVARRIAAQARVLCLDELQVNDIADAMILARLFSALVDRGVTL
                     VFTSNVPPHGLYRNGLQRRRFLPAIALLEKHTEVIAVDGGVDYRLRELARASLYLDST
                     APGSQQELAKLFEFFAGDAGTAGGTIEVAGRAIPVVRANDNAVWFEFDALCEGPRGKD
                     DYLEIAGDYPALLLANVPQFDETRDDAARRFITLIDELYDHGVNLAVSAAAPPDRLYR
                     GTRLAFEFARAASRLVEMGREQYLRREHT"
     gene            complement(12407..14353)
                     /locus_tag="PKN91_04165"
     CDS             complement(12407..14353)
                     /locus_tag="PKN91_04165"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HNS27299.1"
                     /translation="MSTPIWTPSADRIEGAVVTRFRSCVNARLQLALTDYKALEAWSL
                     ENPAAFWRELARFSGTIADWGDGPVLEHGGIMPGARWFPTARLNFAENLLRHRDDHAA
                     LIFVNERGTRRELSRRALHAEVARVADGLRALGVGVGDRVAGYMPNLPETIVAMLATT
                     SLGAVWSSCSPDFGVNGVLDRFGQITPKVLFTADGYFYAGKQLDSLAQIAQIAPQLPG
                     LEAVVVVSYMQATPDIGGVARAVPYASFGRAGTRLSFEQLPFDHPLYILYSSGTTGKP
                     KCIVHGAGGTLLQHQKEHLLHTDLRPDDRFFYYTTCGWMMWNWLASGLASGAALVLYE
                     GSPFHPGPHTLWDMAAAERIAIFGASAKYLAALDKAGFEPAREVDVGALRAVLSTGSP
                     LLPQTYDYVYRAVKADLQLSSISGGTDIVSCFCLGSPVLPVRRGEIQCRGLGMRTEVF
                     DEAGARVIDQRGELVCAAPFPSMPVGFWNDPDGSRYREAYFSRFPNTWHHGDDATETA
                     DGGFVIFGRSDAVLNPGGVRIGTAEIYRQVEQLPEVLESLAIGQQWEGDVRVVLFVRL
                     REGVTLDDALVKRIRDTIRRNTTPRHVPAKVIAVADLPRTLSGKLVELAVRNVVHGKP
                     VLNRDALANPAALDLFRDLPELAD"
     gene            complement(14362..15984)
                     /locus_tag="PKN91_04170"
     CDS             complement(14362..15984)
                     /locus_tag="PKN91_04170"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HNS27300.1"
                     /translation="MTTPSSEAVERDVMEYDFVIVGAGPSGLACAIRLKQLNPDASVC
                     VLEKGAAVGAHSLSGAVMEPEPLDALAPEWRDAQFDVCVPAQRDELRILTRSGARRLP
                     VPPQQRNHGNFIVSLGLLTPWLAQKAEGLGVDVFAGFAGAAPLFDAAGAVAGVQIGDM
                     GLDKNGEPGPNYAPGPEIRARVTVLAEGCRGSLTKQLIQRYSLAAGSDPQTFGLGFKE
                     LWQLPSGRATPGLIQHSVGWPLDNATYGGSFIYHLGGDRVYVGFVVGLDYEDPRLAPF
                     EVLQQFKHHPAVKPLLDGGEIVSAGARTIAAGGWQSLPKLEMPGALIVGDAGGTLNFP
                     KIKGIHQAIRSGALAAEHLVASGGVTGFDALWRASPGGQELRRVRNFKPGFKRGLWFG
                     LANSALEVLTGGRTPWTLHNSDNAAAMKKIDEYTSPDRGWGERTLPPRDRLASVYFAA
                     TTHDEAQPAHLKVADPSICATRCRVEFDNPCTRFCPAGVYEMIDDGQGGKRLQINAAN
                     CVHCKACDIKDPYGIINWTTPEGGSGPNYQTL"
     gene            complement(15996..16763)
                     /locus_tag="PKN91_04175"
     CDS             complement(15996..16763)
                     /locus_tag="PKN91_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNS27301.1"
                     /translation="MKMKDARAVVTGAASGLGNAVARHVVAQGGKVTLLDVQEGPGQA
                     AAAELGASARFAKCDVTSEAEVNAAMETARAFMGGIDLLVNCAGVIGAGRVLGRNGPM
                     AGEFFTKVIHINLIGTFLCDKAAAAIMQQNSPNEDGERGVLIHTSSVAAFEGQIGQAA
                     YSATKAGVMGMTMPIARELAAFGIRCVSIAPGIFRTPMVAGMPQDVQDSLGRQAPFPP
                     RLGKPEEYAQLVQSIFEIPMINGETIRIDGAIRMQPK"
     gene            complement(16846..17625)
                     /locus_tag="PKN91_04180"
     CDS             complement(16846..17625)
                     /locus_tag="PKN91_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNS27302.1"
                     /translation="MASAGIRLEVDPAGVARLTLDRAERRNAFDAAMIGALRAAIEQC
                     ARDTAVRMLVLTGAGAAFCAGADLAHMRAMGTASGQQNHDDALALAEMLAVLDECAKP
                     VLARVNGDAFGGGVGLIACCDIAVGLADARFALSEVKLGLVPATIAPHVVAAIGVRAM
                     RRYALTGERFDGTQARTIGLLHETAVDIPQLDAVVERLVAELLSGGPQAQQEVKQLVN
                     EVAGAPRNDALRRSTSRRLARLRLSQEARTRIEDFLARRQR"
     gene            complement(17629..19209)
                     /locus_tag="PKN91_04185"
     CDS             complement(17629..19209)
                     /locus_tag="PKN91_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNS27303.1"
                     /translation="MARLVSTIDPASDAFRANRAHMERLVAELRTELARAERGGSDEA
                     RARHTARGKLLPRERISTLLDPGSPFLEIAPLAAHGLYDGDAPAAGLIAGVGRVHGRE
                     VLVIANDATVKGGTYYPLTVKKHLRAQQVALENALPCVYLVDSGGAFLPLQDEVFPDR
                     EHFGRIFYNQARMSALGLTQIAVVMGSCTAGGAYVPAMSDETIIVQRQGTIFLGGPPL
                     VKAATGEEIDAETLGGGDVHARRSGVADHLAENDAHALALARASVAALRPRASAWLDL
                     VPGEEPALDPREIAGIVPDDTRHPYDVREIIGRLVDASDFHEFKPEYGTTIVCGFARI
                     HGCPVAIVANNGVLFSESALKAAHFVQLANRRRTPLLFLQNITGFMVGAKYEQAGIAK
                     DGAKMVTAVACATVPKLTVVIGGSFGAGNYAMCGRAYGGRFLWMWPNARISVMGGEQA
                     AQVLASVRRDDGGREAFMDQIRARYETQGHPFYSSARLWDDGIIDPLDTRRVLGLALH
                     LAAHGPAPDAERNGIFRM"
     gene            complement(19221..20414)
                     /locus_tag="PKN91_04190"
     CDS             complement(19221..20414)
                     /locus_tag="PKN91_04190"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNS27304.1"
                     /translation="MSQKAPASSVVIVAAQRTPIGAFQGALAGATATQLGAAACRGAI
                     AAAGIKPDVIQEVIMGCVLPAGLGQAPARQAALGAGIPASTPATTINKMCGSALKAIM
                     MAADQIRAGDTDVVLAGGLESMTNAPYLLPKARGGYRMGHGEVLDHMFYDGLQSPFDG
                     QMMGCFAESTADKYGFSRAEQDAFAAESVRRTLVAVDNGAFAAEISPVTVKGRKGEVV
                     VERDETPFTCNIEKIPGLKPAFRKDGTVTAASSSSIADGAAAAVLMSEAAAQRLGVKP
                     LARVLAYASHAQAPEWFTTAPVGAIRKVLDRLGWKAGDVDLYEINEAFAVVTMAAMRD
                     IGIDHGRVNVNGGACALGHPIGATGARIVSTLVHALRRTGGKRGIASLCIGGGEATAI
                     AIELL"
     gene            complement(20411..21061)
                     /locus_tag="PKN91_04195"
     CDS             complement(20411..21061)
                     /locus_tag="PKN91_04195"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNS27305.1"
                     /translation="MTRPSSPWNVPPADRERQRELKRDAVILAAARAFRERGYHNTSL
                     DDIAAVLHVTKPTLYYYVSNKEQMLFECFREGLGRIAAACGEARDANASGRERLIAVV
                     RGYAEAITSDFGWCMVRAEDQDLSPQMSARIKALKSEIDQDIRRLVRQGISDGSIAAG
                     DPKMVAFAIAGALNWIAHWYRDDASLSASAIADRFIEFFVNGLAPRVSGATAGKSP"
     gene            21115..>23773
                     /locus_tag="PKN91_04200"
     CDS             21115..>23773
                     /locus_tag="PKN91_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNS27306.1"
                     /translation="MSSRTAQQGQPPVTDADPGGLPLRFVSAASLFDGHDAAINMIRR
                     LLQAHGAEVVHLGHNRSVADIVRAAIQEDADAIAVSSYQGGHNEYFAYMVDMLKELGA
                     AHIRVVVGGGGTISPQEIAALEAHGVEKIYTPEDGRRLGLNGMIDDVFERVRRAQKPA
                     YEFAPLNPQDPQHLARTISCLEGALGTATARAQIARAIARNERRAPVIGITGTGGAGK
                     SSLSDELLARFVRFFPGRNIAVVAVDPTRRRSGGALLGDRIRMNSLSSERLFMRSLAT
                     RRRDSATSEALQDVIALYQLAGFDLIIVETAGIGQSDTGIVDIVDHSIYVMTSEYGAA
                     SQLEKIDMLDFADMIVLNKFEKRGAEDALRDVRKQWRRNHPDKMKIADAQVPVYPTIA
                     SRFNDPGVNGLFKALCARLDWQVGDVGPTDFKSHDALIPASRVRYLAEIAQGGREARA
                     RIERQVDAARRAHAHYESLKALHDPQLPAPLDRYPAAALGSEADATRRELRAAYNAAL
                     DEVGADAIGELKAWPARSKAVTDETYSYPVRDRQVTGENYTETLSRNRVPKLAVPRYV
                     DWGEILGFLGNENLPGAYPYTGGVYPYRREAEDPTRMFAGEGPPERTNRRFHLLAGGH
                     EATRLSTAFDSTTLYGEDPDTRPDIYGRTGNSGVSIATLDDMKKLYSGFDLCLPTTSV
                     SMTINGPAPMILAMFMNTAIDQRVERWLRAKGRWSAAEARVAQIRQADAANAALWPAY
                     KGDLPVGHDGSGLALLGVSSEQLIAPGVLDRATWERLRAEALSQVRGTVQADILKEDQ
                     AQNTCIFSTEFALRMMGDVQQFFIDNQVRNFYSVSISGYHIAEAGANPVSQLAFTLAN
                     GFTIVEYYLARGMAIDDFAPNLSFFFSNGM"
BASE COUNT         3673 a         8002 c         8325 g         3773 t
ORIGIN      
        1 caggccgtgc gtggcccatc gcgtatgcgc gatgcccaca caggcgccct cgcgacgctt
       61 gggtggcggt ggcattttgc tcagtgcaaa gcccggtaaa tggcatcggc cagctccgcc
      121 gaaattccgt ccaccgtttg caaatcggcc acgctggcgt ctgccacacc gcgcaccccg
      181 ccaaagcgct ggagcaattt ggcgcgccgc ttggggccca caccggcaat gtcttccagg
      241 cgactgccgc cggtgcgcgc agcgccacgc tcgtccactg cgacctgcac ggctcgaacc
      301 tcattgaggg cgatcgcttg tggctcatcg actgggaata cgccgcgctc gccgatccgc
      361 tgcaggacgt ggccagcgtg ctggcgtact acccgcacgc ggggcggcat tccgggcgcc
      421 tgctcggcgc gctcggcctc gccggcgtca cgccgcgcga gttgctggcc gcggtgtgga
      481 tgttccagct gctcgtgttc ctctggtacc gcgtccgacg ccttgccgtg gcgccgacga
      541 gcgcggatct tgccgccgaa cggcgcgcgc atgcggccct ggtgcataat gggaatcttt
      601 gatccacagg agcgcgaatg tcagtgttga aggtcgtaga ccgcgatggc gtcgaacatg
      661 aagtcgaggc gcagccggga attaaggtca tggaagtgct gcgggaactc gactatggcg
      721 tcgcggcgat ctgcggcgga atgtgttcgt gcgccacctg ccacatctac gtcgaccctg
      781 aatgggtcgg ccgcctgccg gcggcgatgt ccgacgagaa ggacttgttg agcgaactgt
      841 cccatgtgca ggagaattcg cgcctgtcgt gccaggtcga tttcacggcg gcgctcgacg
      901 ggctcaaggt ggtgatcgcc cccgacgaat gaggatgagc gcatgaccga taccgtactg
      961 gtggaaatcc gcggcgacgt ggccgtactg actctgaatc gcccggagag cgccaatacg
     1021 ctcgacctcg acatgggtcg ggacctgctc gctgccgcac tgcgctgcag cagcgatccg
     1081 gcggtgcgcg ccgtggtgct gaccggcgcc ggcaggaatt tctgcttcgg cggcgacctg
     1141 cgcggcatgg tgagcggcgg cgcgccggtc gagccttacc tgcgcgacct gacatcgcac
     1201 ctgcatgcgg cgatctcgca gttcatgcgc atggacgccc ctgtcgtcgc cgcggtcaac
     1261 ggtacggctg ccggcgcagg cgtcgggctc gtcgcgatgg ccgacctcgc gatcgcaggc
     1321 gagggcagca ggatttcgct tgcgtacacc ggcgtcggac tcacgccgga cggcggaacc
     1381 tcgttcctgc tgccgcgcgc ggtgggagcc aagcgcgcca gcgagttgat cctcaccaac
     1441 cgcgcgctca gcgccgccga ggcgctcgag tggggcctcg tcaaccaggt cgtcgcggat
     1501 gccgaagtgt tcgatgccgc gctgcagctc gccggcaagc tggccgcggg tcctcgcgct
     1561 gcgttcggca aggcgaagcg gctgctcggc gcctcgctcg gcgccctcga gcagcagatg
     1621 gcgctcgaga gcgagaccat tgcgattcag gccgcgaccg ccgagggcgg tgagggcatc
     1681 gccgcattcc tcgaaaagcg caagccgcag tacccgaggt agccccgatg gacaatcgcc
     1741 agtggatcct cgtcaagcgc ccgttcggcg aagtcgacga ccagactctg gcgctgcgca
     1801 ccgcaccggt ccgtgaactc gcggacggcg agtgcctgat ccgcaatctg tacctgtccc
     1861 tcgacccgac caatcgcatc tggatgagcg atcaggagca gtacctgcca ccggtcgcga
     1921 tcggagaggt gatgcgcggg ctcaccatcg gccgcgtggt cgcttcacgc aatccacgca
     1981 tcaaggtggg cgaccccgtg cagggcgtcg gcggctggca ggactactgg atcgcccccg
     2041 atggggccgc agcgaagctg cgtgtcgagg gtgacacacc gctgaccctc tacatgggcc
     2101 tgctcggcat ggtcggctgc accgcatatt tcggcatgct cgagatcggc aatccgcagc
     2161 cgggcgagac ggtggtcgtg tcggccgctg ccggcgcggt cggctcgatc gccgtgcagc
     2221 tcgcgaagtt gcgcggcgcg cgcgtcatcg gcatcgccgg cagtgacgag aagtgcggct
     2281 ggctcatcga tgaactcggc atagacgcgg cgatcaatta tcgccgcgag aaagtgcgca
     2341 cccgcctgcg tgaactcgcc ccgaaaggcg tcgacgtgta tttcgacaat accggaggcg
     2401 agatcctcga cgcggtgctc gccaatctcg cgctcaatgc gcgcattccg ctctgcggcc
     2461 tcatttccac ttacaacgcg accggccgcg gcagcgggcc cgcgaactat ggcgcgatcc
     2521 tgatcaagcg tgcgatggtc aagggattca tcatcctcga ctaccaggcg cgttacggcg
     2581 aggcgatcag ggcgcttgcc gactggtacg cgcagggcag gctgaaatac cgcgttgacg
     2641 tcgtggacgg cctcgagtcc gcaccgcgcg cactcggcaa gctgttcgat ggcagcaacc
     2701 agggcaagct cgtcgtgcgc gtcgcagccg atacggcaca ctgaccgcac atcccggcca
     2761 gggcgggacc cagccgcatt tcggctatca ttggccttgg tgaaaccatc atgacgctcg
     2821 cgtatatctt ccttttcttc ggcggcgcga tcgcgatctg gcgcatctgg atcaacatca
     2881 agcgggcgcg cgacaggccc gcgcacagct gggacgaacg gctgatcgag aagctgcgcc
     2941 tcgaaggctc ggatcccttc cagccccacg acgtcgtgtt cttcttcggc ctgccgagcg
     3001 agaagtcggc gcagctcatc gtcgagcgcc tggtgcgcga gggctatcag gccgagtaca
     3061 ggcaggtgcg cgagcaggtc gagctggcct tcagcgtgca cgcgcagcgc tcgctacgac
     3121 tgtcggtgcc cgacatgcag gcgacgagcc ggcgattcaa cgagctggcg gccgaattcc
     3181 acggccgtta tgacggctgg gcggcggcca gcaaccgcag gatcggcgac tgacgcctac
     3241 ttcgcggcgg tctgccgctt gaccgcctcg gcggcggctg cgatcgcggc ggcgtcgccg
     3301 agatagcggc tcgacttcgg tcgcagtgca tcgtcgagct cgtaaagcag cggcacgccg
     3361 gtcgggatgt tgagttcgac gatatcggct tccgacatgt cgtcgagcat cttgaccatg
     3421 gcacgcaggc tgttgccgtg ggcgaccacg gcgacattgc gccctgcccg cagttccggc
     3481 gcgatgcgct cgttccagta cggcagcacg cgcgcgagcg tgtccttgag cgactccgtc
     3541 gccggcagca gcgcggggtc gataccggca tagcgggggt cgaagcgcgg gtggcgcctg
     3601 tcatcgctcg caagcggcgg cggcgggacg tcgtagctgc ggcgccagag tttcacctgc
     3661 gcttcgccgt gccgcttgac cgtctgggcc ttgttgagcc cctgcagcgc gccgtaatgg
     3721 cgctcgttca ggcgccagct gcgttcgacc ggcacccaca tgcgatcggt ttcatcgagg
     3781 atgtaccaca gcgtgcggat cgcgcgcttg agcaccgagg tgaacgcgat atcgatctgc
     3841 aggccttcgc gcttcacctc gacaccggcc tggtgggcct cggcgcgacc ctgcttcgtc
     3901 aggtcgacgt cggtccagcc tgtgaacagg ttttccaggt tccaggtgct ctggccatgt
     3961 cgaacgagca gtagttttcc gggcacgact tgctccgtgt gggtttgggg gggctgaaaa
     4021 cgaaaagctt acaggcacat ttcgccatct tgcatcgtgg attgtgccac ctccgcaagg
     4081 ccgtgctcgc agcgcaccgt gcgttcgcgg cgcgtggcgg cgagaatcgc ctcgttccgt
     4141 gcgcgccggt cgcgtgtcgc cggcgggtga tgcaggtgcc aggcgatgcc gccaaagacc
     4201 agcgcgcgcc ggtggcgccc gagatgcccg aggcgcgcgc acaattcctt gtcttcgggg
     4261 ccccagccga ccatggcgtg gttgaagccg ttcacggcca ggaggtcgtc gcgccaggcg
     4321 gcgaggttgc agctcttggt ggccacgacg gcgttgccga tacgcgcgct gcagcgggcg
     4381 agcgcggggc tgtggagggc ccagatgcgg cgcagcccgc gggcatgccg ggggaccgcc
     4441 ccgggcgcgt cgcgcgccgc agcgagcgcc gccttcgtgg cctcggcgtc gagcggtatg
     4501 cgcacgccct gcacgaagca gcccgggcgc gccgccctgc gatgatcggc gatgaattcg
     4561 gggtgcagca gcatgtcgcc atcgacgaag acgaggtagt cgccgcgtgc cgctgcgatc
     4621 gcgaggttgc gcaggcgcgc ggcgcgaaag ccgtcatggt cctggcggac gtgggccacc
     4681 ggcgcgggcg cctgcgcggc gaatgcggcg acgacctcgc gggtcgccgg gcccgagccg
     4741 tcatcggcga cgatgatttc cgccggccat tgccgctggg ccgccgctgt cgcgagcacc
     4801 gcggcgaggg cgtcgggccg ctcgtacgtc gtgacgacga gcgacaggcg catcaggccg
     4861 atgcgagttt gcggatcgcc tcgacgccga ccgacagcgt cgcgaagtcc ggatgatcgg
     4921 cggcgcgcat gtcggccagc atgcgcgacc agcgcgcgaa ctcgtccccc tgggattgca
     4981 cccacgccgc gaccggatcg cgggccgcgc cacggcggcg catcgagcgc agcacgtcgc
     5041 cggcgagcgc gcgatgtgca cgggccgcgc tgttgcgcag ggtcgagcgg gcgttcgcct
     5101 gccatggccc gctggccgcg agcgcgccga tctgggtgcg caaccagtcg aggccgacgg
     5161 cggcgccgat cgcgtaatag atgcgcgcgg cgtcccgcac ggacttgcgg tgttcggccg
     5221 cgagttcgac caggtcgtac accggttcca tcgattccag cgcgccgata cgcgcggcga
     5281 gatcctcggg caggccggcc gacacgagtt gcgcgcgtcg cgccgcgttg tgctccggca
     5341 cgaggccggt cgtgaccccg tgcagcgcat cgccgagttc gcgcacgccc ggccggtact
     5401 ccgccaccgt gcgctcgacg tgcaggttgc gacgccggta ctgcagcagc cagtagctca
     5461 tgtgccgcag cagccgcgcc gtatagtcgt aggcaagata ctgcgcctgc gccggcacgc
     5521 ggctgtcgag cgcctcgatc tcgctccaga tggcccgcat ctcgaagctc tcgcgcgcga
     5581 tcgtgtaggc gcgcgcgatc tgcccgattg tggcgccggt ctcctcgacc gcgcgccagg
     5641 cgaacaccgg ccccatgcga ttcacgaggc tgttggtggt cgcggtgacg atgatctcgc
     5701 ggcgcagccg gtgcctgtcg atgtcgcgcg cgaacttgcg gcgcagcgcc tcggggaagt
     5761 agcggtgcag ttcctcggac aggtacgggt cctcggggac atccgagtcg atcagttcgc
     5821 ccgacagcca gatcttgctg taggcgagca gcaccgcgag ttccggccga gtgagcccgg
     5881 cgccgcgctt ggcgcgctcg atgaactcgt cttcgctcgg caggaactcg atattgcgat
     5941 ccaggtcgcc tgcgcgctcg agcgaacgga tcagtccgcg atattcgctc aggcgcgcga
     6001 ccgtattgag ctcgagggtc gagatggact ggctctgcag gtagttggtg cgcagtacca
     6061 gcgcgctgat gtccggggtc atgccgcgga gcaggcggtt gcgcgccgcc aggctcagcc
     6121 ggccgctgcg caccgggtcc gcgagcagga tcttgaagtt cacctcgaca tccgaggtgt
     6181 tgacgccggc ggagttgtcg atgaagtcgg tattgatgcg cccgccgttc gcggcgtact
     6241 cgatgcgtcc gcgctgggtg caacccaggt tgccgccttc gccgaccacg cgcgcgcgaa
     6301 gctcgcggcc gttgacgcgc acggcgtcgt tgctgcgatc gccgacttcc gcattgcgct
     6361 cggcctcggc cttcacgtag gtgccgatgc cgccgttcca cagcagatcg acgggcatgc
     6421 gcaggacggc gcggatcacc tcctgcggtg tggcagacga cacgctgacc ccgagggcgc
     6481 gctgcgcctc ggccgacagc gcgatcgact tggcgctgcg cgggaagacg ccgccgccgc
     6541 gggagatcag cttgcggtcg tagtcgtccc atgaggagcg cggcagccgg gccaggcgct
     6601 ggcgctcgag gtagctgcgc gccgcatcgg gctgcgggtc gatgaagatg tgcaggtggt
     6661 tgaacgcggc aaccaggcgg atgtgcttcg agagcagcat gccgttgccg aacacgtcgc
     6721 ccgccatgtc gccgatgccg gcgacggtga agtcctgcga ctgcgaatcg atgccgatct
     6781 cgcgcaggtg acgcttgacg cattcccagg cgccgcgcgc ggtgatgccc atcttcttgt
     6841 ggtcgtagcc ggccgagccg cccgatgcaa aggcatcgcc cagccagaag ccgtagtcga
     6901 ccgaaatcga gtttgcgatg tcggagaagg tcgccgtgcc cttgtcggcg gccacgacga
     6961 ggtagctgtc gtcgccgtca tggcgcacga catcgcgcgg gggcacgact tcggtgccca
     7021 cgatgttgtc ggtgatgtcg agcaggccgc ggatgtaggt ctggtagcag gcaatcgcct
     7081 ctttctggat ctcgtctcgc gtggcgccca ccggcagctg tttcgggacg aatccgccct
     7141 tggcgccggc aggtacgatc acggtgttct tgacgttctg cgccttcatc aggccgagca
     7201 cttcagtgcg aaagtcctcg cgccggtccg accagcggat gccgccgcgc gcgaccgccg
     7261 ccatgcgcag gtgcacgccc tcgacgcgcg gactgtagac gaagatctcg taccttggcc
     7321 gcggcagcgg caggtccggg atccgcgccg gatcgagctt catggagacg tagtccttgg
     7381 acctgcccgc ggcgtcgcgc tggtagaagt tggtgcgcag catcgccttg atgacggcca
     7441 ggtagccacg caggatgcgg tcgtcgtcga gactggtgac ctgtgtcagc gcgcgaccga
     7501 tgccggcgac gatgcgctcg acattgcgcg cacgcgcgcg ctccggggcg gccggatcga
     7561 acctggcgtg aaagagcgcg atgagctggc gcgcgatgcc cgcgtggtgc gcgagcgtgc
     7621 gctccatcga tgcctggctg aagggtacgg cggtctgcac caggtacttg gcgatcatcc
     7681 gcatgaccac aacgtcgcgc gagggcatgt cggcgagcag cacgaggcgg ttgaaaccgt
     7741 cgttctcgat gtcgccgcgc catgccgcgg cgacaccttc gttgaacagc ggctcgacgc
     7801 gatcgatgtc gagctcgatc cggtcgcgat gctcgagctc gaagtcctgg atccaggcct
     7861 cgccgccgtc cggccagctg agttcatagg gccgctcggt gatcacgcgc aggccgaaat
     7921 tctcgagcag cggcagcatg tccgagatag ggatcgtctc gccgagcttg acgaacttca
     7981 gattgacgcg cttcgcgtcc tggcctggcg gacggtgcag gttcaggcgc agcgcgcccg
     8041 gctcgctgcc acgcaggctt tccaggtcgg cgatgtcctc gagcatgtcg ctggccgcga
     8101 cgtcttcctc gtaggcggtc ggaaaggccc tggcgtagcg cgacatcagc gcgagcgcgg
     8161 cggactcgtc gtgctgcgcc ttgagggcgg ccgacagccg gtccatccag ttggtggcag
     8221 cggcggcgat ctcgcgctcc atgcgtgcga cgtcgacgtt cgtgcgctgc ttgcgatcga
     8281 cgcgcacgac cacgtgcacg cgcgcatggc gcgagtcgga aatctggatc tgtgactcga
     8341 aggcgacgcc cttgaagccg gcgagcagga tgcgctcgat ctggtggcgc acgtcggtgc
     8401 tgtagcgatc gcgcggcaca tagacgaggc aggagtagaa ccgcccgtag ggatcgcgcc
     8461 gcgccagcag cctcacggtg cgtcggtcgt acaggttgac gacctcacgc acgatgcgga
     8521 tcaggtcggc gacattcgcc tggaacagct cgtcgcgcgg ataggtctcg agcacgttga
     8581 cgacggcctt ggcgtcgtgg ctctgcggtg cgagcccgaa atggtcgatg acctgctcga
     8641 ccttgcggcg caggaccggt atcgcgcgcg cactgcggta gtacaccgtc gaggtccaca
     8701 ggccgaggat gcggtgctcg ccatcgacgc ggccctgtgc atcgaaggtc ttgacgccta
     8761 catagtcgag ataggtgccg cggtggaccg tcgcgagcgt gttggccttg gtcagtatca
     8821 gcagctccgg ctcgcgcgcg cgctggcgca gttcgccgcg caggcgcacg gtccgggacc
     8881 tgcgcccacg gtgtccctgg cgcagaatgc cgagaccgga gcggcccgcg ggcacgagtc
     8941 tgtcttcgga ggctccccgc accagccggt agcgccgata gccgaggaag acgaagtgct
     9001 gggcttccat ccattcgagc agcgcgcgcg cttcggtgat ttcgtcggcc ggcagcgggg
     9061 tcgcatcggc gagtccggcg gcgagctggc gcacgctgtc gcgcatcggc atccagtcgt
     9121 cgaccgcggc ctgcacgtcg acgatcgtgg cccggatcga ctcctcgagt cgcgcgagcg
     9181 ctgcgggatc gctctgccgg tcgatctcgt agagctgcca ggactcgagg ctcgattgcg
     9241 ggtcgtcgct gtcggcgaca ccgaggaagc ggccgcgtgc gtcacgccgc atcggaatga
     9301 cagggtgcac gatgaagtgc atcgccacct gcgcctgcgc gagcacgatg ccgatcgaat
     9361 cgacgaggaa cggcatgtcc ttggtgacga tttgcacgat cgtgtgcggc gaggcgaagc
     9421 cgtcgcgcgc cgcatcggga ttgaacacgc ggacccgcgc ctgcgcaccg gcaagcgtgc
     9481 ggcccagtgc gaggtgatcg aggccgacgc cggccagatc ggcgggcggc cgcgccgcga
     9541 ggtcgtcttc cgacacgccg cggaagtagg cgcgcaggaa ctgcgcgtcg gcggtccgct
     9601 ggcgcgagct gcctcgcgcg ggggccgctt tcacgatgcg ttcgatcaat gcgagacggg
     9661 cggccggaac gattctggtc acgatggcag gcttcatcgg gcgttgggcg cgaggcgcgc
     9721 gattatacac ggggtcagtt cgcccgcgcg ccgggtgagt ttctcgagca ggcgatcgag
     9781 cgcgcggcgc tcgtcgcggc cgagcactcc gagcagcgca gcctcgtact cgagcacctg
     9841 cggaacgacc cgcgcataca gcgccgcgcc ggtgcgcgac aggcgtagca ccgaacggcg
     9901 ccggtcgcgc cgcgactgtg tgcgcacgat gcgacgggca cgcagcagcg cggcgacggc
     9961 ccggctgacc gcgaccttgt ccatggccgt gcgcgcggcc acgtccgcgg cgctcatgcc
    10021 cggctgttcg ccgagggccg cgatgacgcg ccactcgggg atcgacagcg aatagcgctc
    10081 ggcataggcg cctgaaatcg cgctgctgac gaggttcgcc agcaccgaga gccgataggg
    10141 cacgaagcgg tcgagctgca ggctgggcgt gggcggcatg gcggtttggg ctccagggcg
    10201 cgtggcgggt ataatggttg caattgtaac catttgcggg gtgagcgatg gcggccatcc
    10261 acgacaatcc gatgggcacg gacggcttcg aattcgtcga gtacacggcc ccggaccccg
    10321 aactgctgcg cagccttttt gcacgcatgg gattcccggt gaccgcgcgc catcgcagca
    10381 aggatgtcac cctgcatcgg cagggcgacg tgaacttcct gatcaatgcg gagccggatt
    10441 ccttcggcca gcagttcgcg cgcgaccacg gtccgtcggt gtgcgcgatg gcctttcggg
    10501 tcgccgacgc ggcccgggcc ttcgagcgcg cgctgtcgct cggggcgcgg ccccaccagg
    10561 gcaggatcgg accgatggaa ctcaacattc ccgccatcga gggcatcggc ggctcgctga
    10621 tctacctggt cgaccgctat ggcgacaagt cgatctacga cgtcgatttc cgcccggtgg
    10681 gcgataccgg cgacggcgtc ggcttcacgc gcatcgatca cctgacccac aacgtacacc
    10741 gcggcggcat ggaccgctgg gcgggtttct acgagcgcct gttcaacttc cgcgaaatcc
    10801 gctatttcga catcgaaggc cggcacaccg gcctcttcag ccgcgcgatg accagtccgt
    10861 gcggcaagat ccgcatcccg atcaacgagt cgcaggacga caagtcgcag atcgaggaat
    10921 acctgaggct ctatcacggc gaaggtatcc agcacatcgc gctcgcgacc gatgacatct
    10981 gtcgcacggt cgatgcgctc gccgcgcgcg gcatcgtgtt ccaggacacc cccgacacct
    11041 actacgaggg catcgatgcg cgcgtgccgc accacggcga gagcgtggag gagttgcgcc
    11101 ggcgccgcat cctgatcgac ggcaatcccg atcgcggcga gggcacgctg ctgcagatct
    11161 tcactcaaaa cgtgatcggg ccgatcttct tcgaggtgat ccagcgcaag ggcaacgacg
    11221 gcttcggcga gggcaatttc cgcgcgctgt tcgaatccat cgagctcgac caggtgcgcc
    11281 ggggcgtgat ctagcccgcg ctcgcggcgc gttcaggtgt gttcgcgccg caggtactgt
    11341 tcccggccca tctcgacgag ccggctcgcg gcgcgggcga actcgaacgc gaggcgcgtg
    11401 ccgcgataca gccggtcggg aggcgccgcg gcagaaacgg ccaggttgac gccgtggtcg
    11461 tacaactcgt cgatcagcgt gatgaagcgg cgcgccgcgt cgtcacgcgt ctcgtcgaac
    11521 tgcggcacat tcgccagcag cagggcaggg tagtcacccg cgatttcgag gtagtcgtcc
    11581 ttgccgcgcg gcccctcgca cagcgcatcg aactcgaacc agacggcgtt gtcgttggcg
    11641 cgtaccaccg gaatcgcccg gccggcgacc tcgatcgtcc cgcccgccgt gcccgcgtcg
    11701 ccggcgaaga actcgaacag cttcgccagc tcctgctgcg atccgggggc ggtggaatcg
    11761 aggtacaggg aggccctggc gagttcgcgc agccggtagt cgacgccgcc gtcgaccgcg
    11821 atcacctcgg tgtgcttctc gagcagcgcg atcgcgggca ggaagcgccg ccgctgcagg
    11881 ccatttcgat acaggccatg cggcggcacg ttcgacgtga ataccagcgt gacgccgcga
    11941 tcgaccagcg cgctgaacag cctcgcgagg atcatcgcgt cggcgatatc gttcacctgc
    12001 agctcatcga ggcacagcac ccgcgcctgc gcggcgatgc gccgggcgac tacggtgagg
    12061 ggcgattcgg tctgcgccag cgccgcaagc tgcgcatgga cttcgtgcat gaagcggtgg
    12121 aaatgccggc ggcgccgggc ttcgaacggc aggctggcgt ggaacgcgtc catcagcagc
    12181 gttttaccgc gcccgacgcc gccccacagg tagatgccgc gcccggccct gcgctccggc
    12241 gggcgcagca ggccacgtac acgtgcgagc ggattgcgtc cagcgcgctc ggcggcgacc
    12301 aggcggcgcc gcaggtcctc gagcgcgtcg atggcagcca gctgggccgg gtcgggcacg
    12361 aggccgcgac gccccagttc gcgcgcgaga acggcggttg gcacggtcaa tccgcgagtt
    12421 cgggcagatc ccggaacagg tcgagcgcgg cgggattggc gagcgcgtcg cggttcagga
    12481 ccggtttgcc atggacgaca ttgcgtaccg cgagctcgac gagtttgccg ctcagcgttc
    12541 gtggcaggtc ggccacggcg atcaccttgg ccggcacatg gcgcggcgtg gtgttgcggc
    12601 gaatcgtgtc gcggatgcgc ttcaccagcg catcgtcgag cgtgacaccc tcacgcagcc
    12661 gcacgaacag cacgacgcgc acgtcgcctt cccattgctg gccgatcgcg agggactcga
    12721 gcacctcggg caactgctcg acctggcgat agatttccgc ggtgccgatg cgcacgccgc
    12781 cggggttcag tacggcgtcc gaacgcccga agatcacgaa gccgccgtcg gcggtctcgg
    12841 tggcgtcatc gccgtggtgc caggtgttcg ggaagcgcga gaaatacgcc tcgcgatagc
    12901 gcgagccatc cgggtcgttc cagaagccga ccggcatcga cgggaacgga gccgcgcaca
    12961 ccagctcgcc gcgctggtcg atgacgcgcg cgccggcttc gtcgaagacc tcggtgcgca
    13021 tgccgagccc gcggcactgg atttcgcccc tgcgcaccgg cagcaccggg ctgccgaggc
    13081 agaagcacga gacgatgtcg gtgccaccgg agatcgacga cagctgcaga tcggccttca
    13141 ccgcgcggta gacgtagtcg taggtctggg gcagcagcgg cgagccggtc gacagcacgg
    13201 cacgcagcgc gcccacgtcc acttcgcgtg ccggctcgaa accggctttg tcgagcgcgg
    13261 ccagatactt ggcgctcgcg ccgaagatcg cgatgcgctc ggcggccgcc atgtcccaca
    13321 gtgtgtgcgg cccgggatgg aacggcgagc cctcgtagag cacgagcgcc gcgccggagg
    13381 cgaggccgct ggcgagccag ttccacatca tccagccgca ggtcgtgtag tagaagaagc
    13441 ggtcgtccgg ccgcaggtcg gtgtgcagca ggtgttcctt ctggtgttgc agcagcgtgc
    13501 cgcccgcgcc gtggacgatg cacttcggct tgccggtggt gccggaggaa tagaggatgt
    13561 agagcggatg gtcgaagggc agttgctcga acgagagccg cgtgccggcg cggccgaagc
    13621 tcgcataggg cacggcccgc gccacaccgc cgatgtccgg tgtcgcctgc atgtacgata
    13681 cgacgacgac cgcttcgagc ccgggcagct gcggggcgat ctgcgcgatc tgcgcgagcg
    13741 agtcgagctg cttgccggca tagaaatagc cgtcggccgt gaacagcacc ttgggcgtga
    13801 tctgcccgaa gcggtcgagc acgccattga cgccgaagtc cggcgagcac gagctccata
    13861 ccgcgccgag gctggtcgtc gcgagcatcg cgactatggt ctccggcagg ttgggcatgt
    13921 agcccgcgac gcgatcgccg acgcccacac cgagcgcgcg caggccgtcg gcgacgcgtg
    13981 caacttcggc atgcagcgcg cggcgcgaca gttcgcgccg tgtgccgcgc tcgttcacga
    14041 atatcagtgc cgcgtggtcg tcacggtgac gcagcaggtt ctcggcgaaa ttgaggcgcg
    14101 cggtcgggaa ccagcgcgca cccggcatga tacccccgtg ttcgagtacc gggccgtcgc
    14161 cccagtcggc gatcgtgcca ctgaaacgcg cgagttcccg ccagaacgca gcggggttct
    14221 cgagcgacca ggcttcgagc gccttgtagt ccgtcagcgc gagctgcagg cgcgcgttga
    14281 cgcacgagcg gaaccgggtg acgaccgcgc cttcgatgcg atcggccgag ggagtccaga
    14341 tcggagtgct catgcgccga gtcaaagagt ctggtagttg ggtcccgaac cgccttccgg
    14401 cgttgtccag ttgatgatgc cgtagggatc cttgatgtcg caggccttgc agtgtacgca
    14461 gttggccgcg ttgatctgca ggcgtttgcc accctggccg tcgtcgatca tctcgtagac
    14521 acccgccgga cagaaacgcg tgcacggatt gtcgaactcg acgcggcagc gcgttgcgca
    14581 gatgctcggg tcggcgacct tcaggtgcgc gggctgcgcc tcgtcgtgcg tggtagccgc
    14641 gaagtacacc gaggcaaggc ggtcgcgcgg cggcagcgtg cgctcgcccc agccgcgatc
    14701 gggcgaggtg tactcgtcga tcttcttcat ggccgcggca ttgtcggagt tgtgcagggt
    14761 ccatggggtg cgtccgccgg tgagcacctc gagcgcggaa ttcgcgagtc cgaaccacag
    14821 gccccgcttg aagcccggct tgaagttgcg cacgcgtcgc aactcctggc cgccgggcga
    14881 ggcgcgccac aatgcgtcga agccggtcac tccgccgctc gccacgaggt gttccgcggc
    14941 cagcgcaccg gagcggatgg cctggtgaat gcccttgatc ttcgggaagt tgagggtgcc
    15001 gccggcatcg ccgacgatca gcgcgccggg catttcgagt ttcggcaggg actgccagcc
    15061 gccggccgcg atcgtgcgtg cacccgccga gacgatctcg cccccgtcga gcagcggctt
    15121 caccgccgga tggtgcttga actgctggag cacttcgaac ggcgcgagcc gcgggtcctc
    15181 gtaatcgagc ccgacgacga agccgacgta cacgcggtcc ccgcccaggt ggtagatgaa
    15241 gctgccgccg taggtggcgt tgtcgagcgg ccagccgacg ctgtgctgga tcagccctgg
    15301 cgtggcccgg ccggacggca actgccacag ctccttgaag ccgagtccga aggtctgcgg
    15361 atcgctgccg gcggcaagcg agtagcgctg gatcagctgc ttcgtgaggc tgccgcgaca
    15421 gccctcggcg agcaccgtga ccctggcgcg gatctcgggg cccggggcgt agttcgggcc
    15481 cggctcgccg ttcttgtcga gtcccatatc gccgatctgc acgcccgcca cggcccccgc
    15541 ggcgtcgaac agcggcgcgg cgccggcgaa gcccgcgaac acgtcgacgc ccaggccttc
    15601 cgccttctgc gcgagccagg gtgtcagcag gccgagagac acgatgaagt tgccgtgatt
    15661 gcgctgctgc ggcggaaccg gcaggcgacg cgcgccgctg cgggtcagga tgcgcagttc
    15721 atcgcgctgc gcgggcacgc agacgtcgaa ctgcgcatca cgccattccg gcgcgagcgc
    15781 atcgagcggc tccggttcca tgaccgcacc cgacagcgaa tgcgcgccca cggccgcgcc
    15841 cttctcgagc acgcagacac tggcgtccgg gttgagttgc ttcaggcgga tcgcgcaagc
    15901 gagccccgac gggcccgccc cgacgatcac gaagtcgtac tccatgacgt ctcgttccac
    15961 ggcttcgctg ctcggtgtcg tcacggtgtg tcctcttact tcggttgcat cctgatcgcg
    16021 ccgtcgatgc ggatcgtctc gccattgatc atcgggatct cgaagatcga ctgcacgagc
    16081 tgtgcgtact cctcgggctt gccgagcctg ggcgggaagg gcgcctgcct gccgagcgag
    16141 tcctggacgt cctgcggcat gcccgcgacc atcggcgtgc ggaagatgcc cggtgcgatc
    16201 gacacgcagc ggatgccgaa cgccgcgagc tccctcgcga tgggcatcgt catgcccatg
    16261 acgccggcct tggtggccga ataggccgcc tggccgatct ggccttcgaa cgccgccacc
    16321 gacgaggtgt ggatcagcac gccgcgctcg ccgtcctcgt tcggcgagtt ctgctgcatg
    16381 atggcagccg cggccttgtc gcacaggaac gtgccgatca ggttgatgtg gatgaccttg
    16441 gtgaagaatt ccccggccat cgggccgttg cggccgagca cgcggcccgc gccgatgaca
    16501 ccggcgcagt tgaccagcag gtcgatgccg cccatgaacg ccctggcggt ctccatcgcg
    16561 gcgttcactt cagcctccga ggtgacgtcg cacttggcga agcgcgcgct cgcgccgagt
    16621 tcggcggcgg cggcctggcc ggggccttcc tgcacgtcga gcagggtgac cttgccgccc
    16681 tgggcaacca cgtggcgtgc gacggcattg ccgaggcccg atgctgcgcc cgtgacgacg
    16741 gcgcgcgcgt ctttcatctt cattcagaat cctctggagt gggcggaaaa aaatggggtc
    16801 tgacagtaaa gcacaggcgg ccgatgcggg tacagactgc gaccttcagc gctggcgccg
    16861 cgcgaggaaa tcctcgatgc gcgtgcgtgc ttcctgcgag aggcgcagtc gcgcgaggcg
    16921 ccggctcgtc gagcgtcgca gtgcgtcgtt gcgcggcgca cccgcgacct cgttgaccag
    16981 ctgcttcact tcctgctggg cctgcgggcc acccgagagc agctcggcga cgaggcgctc
    17041 caccaccgcg tcgagctgcg ggatatcaac cgcggtttcg tgcagcagtc cgatcgtgcg
    17101 ggcctgcgtg ccgtcgaagc gctccccggt cagcgcgtag cgccgcatcg cgcgcacgcc
    17161 gatcgcggcc acgacatgtg gtgcgatcgt cgccggcacg aggccgagct tcacctcgga
    17221 gagcgcaaag cgcgcgtcgg cgaggcccac cgcgatgtcg cagcaggcga tcaggcccac
    17281 accgccaccg aaggcgtcgc cgttcacgcg cgccagcacc ggcttggcgc actcgtcgag
    17341 caccgcgagc atctccgcga gcgcgagtgc atcgtcatgg ttctgctgcc cggaggccgt
    17401 gcccatcgcg cgcatgtgcg cgagatcggc gcccgcgcag aaggctgcgc cggcaccggt
    17461 cagcaccagc atgcgcacgg cggtatcccg tgcgcactgc tcgatggcgg cgcgcagcgc
    17521 gccgatcatc gcggcatcga acgcgttgcg ccgctccgcg cgatcgagcg tcaggcgggc
    17581 gacgcccgcc gggtccactt cgaggcgtat tcctgccgat gccaccggct acatccggaa
    17641 aatgccgttg cgctcggcgt cgggcgccgg gccgtgcgcg gcgaggtgca gcgcgaggcc
    17701 cagcacgcga cgcgtatcga gcgggtcgat gatgccgtcg tcccacaggc gtgccgacga
    17761 atagaacgga tggccctgtg tctcgtagcg cgcccggatc tggtccatga acgcctcgcg
    17821 cccgccgtcg tcgcgccgca cgctcgcgag cacctgcgcg gcctgctcgc cgcccatgac
    17881 cgagatgcgc gcgttgggcc acatccacag gaagcgcccg ccgtaggcac ggccgcacat
    17941 cgcatagttg cctgcaccga aggagccgcc gatgaccacg gtgagcttgg gcacggtggc
    18001 gcaggcgacc gcggtgacca tcttggcgcc gtccttggca atgccggcct gctcgtactt
    18061 cgcgccgacc atgaagccgg tgatgttctg caggaacagt agcggcgtgc ggcgccggtt
    18121 cgcgagctgc acgaagtgcg cggccttgag cgcggattcc gagaacagca cgccgttgtt
    18181 ggcgacgatg gccaccgggc agccgtggat gcgcgcgaag ccgcacacga tcgtcgtgcc
    18241 gtattcgggc ttgaactcgt ggaaatcgga ggcgtcgacg agacggccga tgatctcgcg
    18301 cacgtcgtag gggtggcgcg tgtcgtccgg cacgatgccg gcgatctcgc gcggatcgag
    18361 cgcgggttcc tcgcccggca cgaggtcgag ccaggccgat gcgcgcggtc gcagtgccgc
    18421 gaccgacgcg cgcgcgagcg caagcgcgtg cgcgtcgttc tcggcgaggt gatcggcgac
    18481 gccggagcgg cgcgcgtgga cgtcgccgcc gccgagcgtc tcggcgtcga tctcctcgcc
    18541 ggtcgcggct ttcacgagcg gcgggccgcc gaggaagatc gtgccctgtc tctgtacgat
    18601 gatcgtttca tcggacatcg ccggcacgta ggcgccgccg gccgtgcagc tgcccatcac
    18661 gacggcgatc tgcgtgaggc cgagcgcgga catccgcgcc tggttgtaga agatgcgccc
    18721 gaagtgctcg cggtcgggga agacttcgtc ctgcagcggc agaaaggcgc cgcccgagtc
    18781 gacgaggtag acgcacggca gggcgttctc gagcgcgacc tgctgggcgc gcagatgctt
    18841 cttcaccgtg agcgggtagt aagtgccgcc cttgaccgtg gcatcgttcg cgatcaccag
    18901 cacttcgcga ccgtggacgc ggccgactcc ggcgatcagc ccggcggccg gcgcatcgcc
    18961 gtcgtagagg ccgtgtgcgg cgagcggcgc gatctcgagg aaggggctgc cggggtcgag
    19021 cagggtgctg atgcgctcgc gcggcagcag cttgccgcgt gccgtgtggc gcgcccgggc
    19081 ctcgtcgctg ccgccgcgtt cggcgcgcgc cagctcggtg cgcagttccg cgaccaaccg
    19141 ctccatgtgg gcgcggttgg cgcggaatgc gtcgctggcc ggatcgatcg tcgaaacgag
    19201 gcgcgccatg gcgcggcagc ttacagcagc tcgatcgcga ttgccgtagc ctcgccgccg
    19261 ccgatgcaca gcgaggcaat gccgcgcttg ccgcccgtgc ggcgcagcgc gtgcacgagc
    19321 gtcgacacga tgcgcgcgcc ggtggcgccg atcgggtggc cgagcgcgca ggcgccgccg
    19381 ttgacgttga cgcgcccatg gtcgatgccg atgtcgcgca tcgcggccat ggtgaccacc
    19441 gcgaacgcct cgttgatctc atagaggtcg acatcgccgg ccttccagcc gagccggtcg
    19501 agcaccttgc ggatcgcgcc gacgggtgcg gtggtgaacc actccggcgc ctgggcgtgg
    19561 ctggcgtagg cgagcacgcg cgcgagcggt ttcactccga ggcgctgcgc ggccgcctcg
    19621 ctcatcagca ccgccgccgc ggcgccgtcc gcgattgacg aggacgacgc ggccgtgacc
    19681 gtaccgtcct tgcggaacgc gggcttcagg ccggggatct tctcgatgtt gcaggtgaac
    19741 ggtgtctcgt cgcgctcgac cacgacctcg cccttgcggc ccttgacggt caccggcgag
    19801 atctcggcgg cgaatgcgcc gttgtctacg gccacgagcg tgcggcgcac ggactccgcc
    19861 gcgaaggcgt cctgctcggc ccgcgagaag ccgtacttgt cggccgtgct ctccgcgaag
    19921 cagcccatca tctggccgtc aaacgggctc tgcaggccgt cgtagaacat gtggtcgagc
    19981 acttcgccgt gccccatgcg atagccgccg cgcgccttcg gcagcaggta gggcgcattg
    20041 gtcatcgact cgagaccacc ggcgagcacg acgtcggtat cgcccgcgcg gatctggtcg
    20101 gcggccatca tgatcgcctt cagcgccgag ccgcacatct tgttgatggt cgtcgccggc
    20161 gtactcgccg gaatgcccgc accgagcgcg gcctgacgcg ccggtgcctg gccgaggccg
    20221 gccggcagca cgcagcccat gattacttcc tggatcacgt cgggcttgat gcctgccgcg
    20281 gcgatcgcgc cgcggcaggc ggcggcgccg agctgcgtgg cggtggcgcc ggcgagtgcg
    20341 ccctggaatg cgccgatggg cgtgcgctgg gcggcgacaa tgacgacgga ggaggcgggg
    20401 gctttctggc tcatggggac tttcctgcag tggcaccgct cacgcgcggt gcgaggccgt
    20461 tgacgaagaa ctcgatgaag cggtccgcga tcgcgctcgc cgacagcgaa gcgtcatcgc
    20521 ggtaccagtg tgcaatccag ttgagcgcgc cggcgatcgc gaaggcgacc atcttggggt
    20581 caccggcggc gatcgagcca tcgctgatgc cctggcgcac aaggcgccgg atgtcctggt
    20641 cgatctccga cttgagcgcc ttgatgcgcg cgctcatctg cgggctcagg tcctggtctt
    20701 cggcgcgcac catgcaccag ccgaaatccg aagtgatcgc ctcggcgtag ccgcgcacga
    20761 ccgcgatcag gcgctcgcgc ccgcttgcat tggcatcgcg cgcctcgcca cacgccgctg
    20821 cgatgcgccc gagtccctcg cggaagcact cgaacagcat ctgctccttg ttcgagacgt
    20881 agtagtagag cgtcggcttg gtgacatgca gcaccgcagc gatgtcgtcg agcgaagtgt
    20941 tgtggtagcc gcgctcgcga aaggcgcgcg cggccgccag gatgaccgcg tcgcgcttca
    21001 gctcgcgctg gcgttcgcgg tcggccggcg gcacattcca gggtgagctt ggcctcgtca
    21061 tgttcccgcg attatacttg ccggtataaa tcctaccagt cggtaacatt acccatgagc
    21121 agccgcacgg cccagcaggg acagcccccc gtcaccgacg cggatccggg ggggctgccg
    21181 ctgcgcttcg tcagcgccgc ctcgctgttc gacggccacg acgctgcgat caacatgatc
    21241 cgccggctgt tgcaggcgca cggcgccgag gtcgtgcacc tcggtcataa ccggagcgtc
    21301 gccgacatcg tgcgcgcggc catccaggaa gacgccgacg cgatcgcggt gagctcctac
    21361 cagggcggcc acaacgaata cttcgcctac atggtcgaca tgttgaagga gttgggcgcg
    21421 gcacacatcc gcgtggtcgt cggcggcggg ggcacgattt caccgcagga gatcgccgcg
    21481 ctcgaggcgc atggcgtcga gaagatctat acgcctgaag atggccgccg cctgggcctg
    21541 aacggcatga tcgacgacgt gttcgagcgc gtgcggcgtg cgcagaagcc cgcctacgag
    21601 ttcgcgccgc tcaatccgca ggacccgcag catcttgcgc gcactatctc gtgcctcgag
    21661 ggcgcgctcg gcaccgccac cgcgcgcgcg cagatcgcgc gcgcgatcgc gcgcaacgag
    21721 cgccgtgcgc cggtgatcgg catcaccggt accggtggag cgggcaagtc gagcctcagc
    21781 gacgaactgc tggcgcgctt cgtgcgcttc ttcccggggc gcaacatcgc ggtggtcgcg
    21841 gtcgacccga cccgccggcg ctccggtggc gcgctgctcg gcgatcgcat tcgcatgaac
    21901 agcctctcct ccgagcggct gttcatgcgt tcactcgcca cccggcgtcg cgacagcgcg
    21961 accagcgagg cgctgcagga cgtgatcgcg ctctatcagc tcgcgggctt cgacctgatc
    22021 atcgtcgaga ccgccggcat cggccagagc gacaccggca tcgtcgacat cgtcgatcac
    22081 tcgatctacg tgatgacgag cgaatacggc gcggcgagcc agctcgagaa aatcgacatg
    22141 ctcgacttcg ccgacatgat cgtgctcaac aagttcgaga agcgcggcgc cgaggatgcg
    22201 ctgcgcgacg tgcgcaagca gtggcggcgc aaccatcccg acaagatgaa gatcgcggac
    22261 gcgcaggtac ccgtctaccc gaccatcgcc agtcgcttca acgacccggg cgtcaatggc
    22321 ctcttcaagg cgctctgcgc gcggctcgac tggcaggtgg gcgacgtcgg gccgaccgac
    22381 ttcaagtcgc acgatgcctt gattcccgcg agccgcgtgc gctacctcgc cgagatcgcc
    22441 cagggtggcc gcgaggcccg cgcacgcatc gagcggcagg tcgatgccgc gcggcgggcg
    22501 cacgcgcatt acgaatcgct caaggccttg cacgatccgc agctgccggc gccgctcgac
    22561 cgttatcccg ccgccgcgct cggcagcgag gcggacgcca cgcggcgtga gctgcgcgcg
    22621 gcctacaacg ccgcgctcga cgaggtgggc gccgatgcca tcggcgagct caaggcctgg
    22681 cccgcgcgca gcaaggcggt gaccgacgag acctattcct atccggtgcg cgaccgtcag
    22741 gtcaccggcg agaattacac cgagacgctg agccgcaacc gcgtgccgaa gctcgcggtg
    22801 ccgcgctacg tcgactgggg cgagatcctc gggtttctcg gcaacgagaa cctgccgggc
    22861 gcctacccgt acaccggcgg tgtgtatccg tatcggcgcg aagccgagga cccgacccgc
    22921 atgttcgccg gcgaagggcc gcccgagcgc accaaccggc gcttccacct gctggccggc
    22981 ggccacgagg cgacgcgcct gtcgaccgcc ttcgattcga ccacgctcta cggcgaggat
    23041 ccggacacgc gcccggacat ctacgggcgc accggcaatt cgggcgtctc gatcgccacc
    23101 ctcgatgaca tgaagaagct ctactcgggc ttcgacctct gcctgccgac gacgtcggtg
    23161 tcgatgacga tcaacggccc ggcgccgatg attctcgcaa tgttcatgaa caccgcgatc
    23221 gatcagcgcg tcgagcgctg gctgcgcgcg aaggggcgct ggtcggcagc cgaggcgcgc
    23281 gtcgcgcaga tcaggcaggc cgacgccgcg aacgccgcgc tgtggcccgc ctacaagggc
    23341 gatctgccgg tcggccacga cggttcggga ctagcgctgc tcggcgtgag cagcgagcag
    23401 ctcatcgccc ccggcgtgct cgatcgcgcg acctgggagc ggctgcgcgc cgaggccctg
    23461 tcgcaggtgc gcggcaccgt gcaggccgac atcctgaagg aggaccaggc gcagaacacc
    23521 tgcatcttct cgaccgagtt cgcgctgcgc atgatgggcg acgtgcagca gttcttcatc
    23581 gacaaccagg tgcggaattt ctactcggtg tcgatctcgg gctatcacat cgccgaggcc
    23641 ggtgcgaacc cggtgagcca gctggcgttc acgctggcga acggcttcac catcgtcgag
    23701 tactacctgg cgcgaggcat ggcgatcgac gacttcgcgc cgaacctgtc gttcttcttc
    23761 tcgaacggca tgg
//
LOCUS       DAOQVY010000027        23639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_2516_length_23639_cov_6.354605, whole genome shotgun sequence.
ACCESSION   DAOQVY010000027 DAOQVY010000000
VERSION     DAOQVY010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 23639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23639
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2516_length_23639_cov_6.354605"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="PKN91_04205"
     CDS             <1..1186
                     /locus_tag="PKN91_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HNS27307.1"
                     /translation="RELDDTTVGIAYLDAICSGSSASLTEARFGKLSSVLVSLVAAHE
                     IGHNFGAPHDGESGKACASTPTTFLMAPTLSTQNSEFSQCSLTQISQRIASAACLVSV
                     DQADVEIVAPVSTVRHGPGENYSLTFTVRSIGTRTAELVSATFVVPPGSSLDAINAAG
                     GTCTNFGAQGNCSLGSLAPGTNRTVTLSLTGHAIGTETLNAVVFSSNDEAPGNNSVSV
                     EVVTDPIADIRVSLTATPASLVVGDDTTVTVTLANLGPAAASDARLSVDVPAGLTIGA
                     INAGGLDCGAGANSVSCNPVALAVDESRSLTVTLRAATAGTRTVSALALSSALDPDSG
                     NNSASVALTIGAAGGGGVPSPPSSGASGGGGGGATSTLLLGLLALALGMRARLAGARA
                     AG"
     gene            complement(1183..4644)
                     /locus_tag="PKN91_04210"
     CDS             complement(1183..4644)
                     /locus_tag="PKN91_04210"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HNS27308.1"
                     /translation="MVTPQVSGIRSLLVANRGEIAIRVMRAAAELGIRTIAIYSQEDR
                     FSLHRTKADEAYLVGRGKGPVDAYLDIEDVLRIAREAQADAIHPGYGFLSENPQFAEA
                     CERDGVIFVGPTPQIMRTLGNKVSARNAAASVGVPVMPATPALPPDPAAAQTLAREIG
                     YPVMLKASWGGGGRGMRVIENDAQLAELLPVARREAQAAFGNDEVYLEKLVRRARHLE
                     VQILGDRYGNLVHLFERDCTVQRRNQKVVERAPAVFLTDAQRTQLCEAGLKLGRAVNY
                     LNAGTVEFLQDADTGEFYFIEVNPRIQVEHTVTECAVGIDIVKAQIRIAAGARIGTPE
                     SGVPPQHEIRMQAHALQCRVTTEDPENNFIPDYGRVTAYRSPAGFGIRLDAGTAYAGA
                     IITRSYDSLLVKITAWAQTAEEASARMHRALWEFRIRGVVTNLRFLDQVITHPAFASG
                     EYTTRFIDSTPELFRFPRKRDRATRMLTYLAETVVNGNAETKGRPRPARMGLARLPKV
                     SLKADPPAGAKQKLDELGPEKFARWMLAERRVLLTDTTMRDAHQSLLATRMRTIDMAA
                     IAPYYARMLPELLSVECWGGATFDVAMRFLKEDPWERLAQFREGMPNLLLQMLLRASN
                     AVGYTNYPDNVVRYFVRQAAACGVDLFRVFDSLNWVENMRVAMDAVLETGRLCEAAIC
                     YTGNLSDPREKKYTLDYYVSLARELKAAGAHIIAIKDMAGLCRPAAARALVTAIKSET
                     GLPLHFHTHDTSGIAAASVLAAAEAGCDAIDGAIDAMSGLTSQPNLGSIAEALRHGER
                     DPGIDTAQLRRLSSYWEQVRRGYAAFESDIRSGASEVYVHGMPGGQYTNLREQARALG
                     VDEARWPDVAQAYADVNDMLGDIIKVTPTSKVVGDMAILMVTSGLTRAQVEDPDTQIA
                     FPESLVQLMRGDLGQPLGGFPQGQQKKVLKDIEPLTVRPGKVLPPIDLEATRAEVARK
                     VGREITDYQLASYLMYPRVFLEYAKDRAAYGDVGRLPTPAFFYGMEPGQEISIDIERG
                     KTLIVNYVAVSETHDDGTRTVFFELNGEPRAIRVADRSQVARRPPQRKAESGNTRHVG
                     APMPGSVATVAVTVGQKIARGDVVVTLEAMKMETAVRAEQDGQVAEVLARPGMQVDAK
                     DLLVVIG"
     gene            4888..5805
                     /locus_tag="PKN91_04215"
     CDS             4888..5805
                     /locus_tag="PKN91_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HNS27309.1"
                     /translation="MHAIAIHGGAGTLPRAEMTPEREAAYRGGLAAALDAGYAVLDAG
                     GTSLDAVIAAVRILEDDPLFNAGRGAVLTREGIAELDASIMNGASLAAGAVAGVRHVK
                     NPIELARAVMEHSAHVMLIGAGAEEFALARGLTLVPNEYFRTETRKAQLQRVLTGDTR
                     PANELSALGTVGAVALDRAGNLAAATSTGGMTAKRYGRVGDSPIIGAGTYADNRSCAV
                     SATGHGEFFIRAAVAHDICARVAYGGRSLAEAADAVVMDRLVTLGGEGGVIAIDPQGR
                     IAMPFNSEGMFRGSRSAGGERVIAIYRDR"
     gene            complement(5802..7019)
                     /locus_tag="PKN91_04220"
     CDS             complement(5802..7019)
                     /locus_tag="PKN91_04220"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNS27310.1"
                     /translation="MPTAAMPEESESPVARTLRAGFPWLRFPGELEREFVAEYRTSRR
                     RIVRIGVCLALTTIIGLAILDRWLLGPGAQVVPDAIRFGVHIPLVVTGIVLTSRRYFR
                     WYWPFVHVFAPLYGLATVLLAANAPEPLTAFIHARLVLAIFFFYFMLGLSFKAALRTN
                     LLTLAGFVVAAFVGDIAPQTSIYQFFLLLFANLYAGAGSYALEHANRVSFLEGRMLRE
                     VATHDGLTQLLNRSALESEIHRIWQQAIRDREHVTVLMIDIDHFKAYNDRYGHQGGDA
                     CLRAVAGAIRRAAGTRARDCVARYGGEEMIAILPGADRAGGEQVARAMLEEVAALAIP
                     HDRSLTAPMVSVSIGVATMLPGRDSSHDLIVRIADRALYLAKEQGRNRHAVLGEADLG
                     EATVVLAARELRA"
     gene            complement(7023..7217)
                     /locus_tag="PKN91_04225"
     CDS             complement(7023..7217)
                     /locus_tag="PKN91_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27311.1"
                     /translation="MNTSKPFEADGHASAALVAANEPTISNVPGRERAHEATPTRGWD
                     PYDVWRTRVKIPRESDNPVA"
     gene            complement(7348..8928)
                     /gene="gshA"
                     /locus_tag="PKN91_04230"
     CDS             complement(7348..8928)
                     /gene="gshA"
                     /locus_tag="PKN91_04230"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HNS27312.1"
                     /translation="MAAPAIDRTFERRVAALVNGGGARLLQGGRRGVEKESLRVTPEG
                     RLATTPHPRALGSALTNGQFTTDYSEALIELVTPTFTTNWELLQYLCDMHQFVYRHIG
                     SELLWATSMPCRIESDDAIPIAQYGSSHVGRMKTVYRNGLGNRYGRAMQAISGVHFNY
                     SFPEQFWEPYAALRQCADEKALRSAAYFDLLRNYRRHGWLVLYLFGTSPAVCNSFLRV
                     RGETLPQLAHGTAYEPYATTLRMSDLGYRNRKQARVSVSVNSLEEYVRDLTRAISTPF
                     PPYEAIGVKVGGEWRQLNANLLQIENEFYSSIRPKRVARSGERPTRALLRGGVEYVEV
                     RALDVSAFDPVGVNQVNLRFLEAFLALCLLRDSPPIGNDEQELLDMNHVVAARRGREP
                     GLRLQRDGRAVPLADWAEELLDGMTGLCEILDDGHAARPYSAALAAQRDKLRDPALTP
                     SARLLHEIRTTGESFFDVAQQMSSAHKSYFLELHPPNAERLAEFQREAEDSVAAQQKI
                     ESADAGSFEDYLRRYFEN"
     gene            9186..9554
                     /locus_tag="PKN91_04235"
     CDS             9186..9554
                     /locus_tag="PKN91_04235"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HNS27313.1"
                     /translation="MRTGKLFAALLLCASGFTAAQADNMRCGQRLVVDGTTRDQVATW
                     CGEPTEVLQRTVLRPPIVWYYGRPVHVGGGHIEVLVETWIYNLGPNKLMRRVIFEDGL
                     VVEVETLGYGYRGSPPAREN"
     gene            9607..10905
                     /locus_tag="PKN91_04240"
     CDS             9607..10905
                     /locus_tag="PKN91_04240"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNS27314.1"
                     /translation="MLAGPAIRRVAIVGGQRIPFARAHGAYATVGNQDMLTAALRAVV
                     EKYGLAGVRLGDVIAGATIKHSKDFGLVRECVLSSGLDPQTPGLDLQRACGTSLEAAI
                     LVANKIALGQIDAGIAGGVDTVSDPPIVYPRSYQQKLLRSYRGRSFGQRIAPFFGLRP
                     RDFKPVMPAVIEPRTGMSMGEHCEQMAKTWAITREAQDQLAYESHMKAAAAWKDGFYD
                     DLVVDFQGLKTDNNIRADTSLDKLAKLAPSFDRQAGTLTAGNSTPLTDGASAVLLASE
                     DWARSRNLPVLAWFSHGKTSAVDYVGGHEGLLMAPVHAVPAMLRDANLTLQDFDFYEI
                     HEAFEAQVLCTLAAWESADYCRERLGLEQPLGSIDRGRLNLRGGSVAIGHPFAATGTR
                     IVASLAKQLAEAPGAKRGLISVCTAGGQGVAAVLERSSDS"
     gene            10947..12074
                     /locus_tag="PKN91_04245"
     CDS             10947..12074
                     /locus_tag="PKN91_04245"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNS27315.1"
                     /translation="MNRYVGFPRVEGSASRQAHADLPAGTFEREISKEGFFGPAAHLY
                     HRHPPTGWVSFEGPLRPHAFDLTRLAAAAPSPWDAPPILGNASVEIRWWQLEGAMPAL
                     ARNADGDQLIFIHRGHGALYCDFGHLDFEAGDYLLLPRGTMWRLECAAPVAALLVEAT
                     NTSYMLPDRGLLGPHAIFDPAILDTPRIDSAFEAQQSSSQPWRVEVKRRGARSVIGYP
                     FNPLDAVGWHGDLAPVRLNVRDIRPLMSHRYHLPPSAHTTFVAGRFVVCTFVPRPFET
                     DPGAIKVPFFHNNDDYDEVLFYHAGDFFSRDNIRPGMMTLHPAGFTHGPHPKALANIA
                     TQAKPATDEFAVMIDTRDAIDIAPAAAGVEWAGYVDSWKGA"
     gene            12071..13051
                     /locus_tag="PKN91_04250"
     CDS             12071..13051
                     /locus_tag="PKN91_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNS27316.1"
                     /translation="MKLASLKSGRDGRLAVVSRDLTRCALADGIAATLQQALDDWQRT
                     APALAELAVRLEAGTVAAARPFDPRDCAAPLPRAHHWVDGSAYVNHVELVRKARGAEL
                     PASFWTDPLLYQGGSDDLLGPTDDAAFGSEEFGIDLEAEVAVIVDDVPMGCSAADAAE
                     RIRLLLLVNDWSLRNLIPGELAKGFGFYQSKPATAFSPVAVTPDELGEAWHEGKLHLP
                     LVSHVNGARLGAPDAGTDMTFGFPQLIAHAARTRRLGAGAIVGSGTVSNYDRSRGSSC
                     LAEKRMLEQIARGEAQTPFLRYGDRVRIEMFDAAGASIFGAIDQRVVPAA"
     gene            13096..13761
                     /locus_tag="PKN91_04255"
     CDS             13096..13761
                     /locus_tag="PKN91_04255"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNS27317.1"
                     /translation="MGEYVTLMARDGHEFQAWLAAPKGTPRGAVVVVQEIFGVNRHIR
                     AVTDAYAAQGYTAIAPALFDRVRRGIELGYTADTMQEGIGYMMQMKPEHFLADVSAAI
                     NAVKSSGRVGIVGYCWGGTVAWLAACELPVAAAASYYGGGIVKMLDRKPARPVIYHFG
                     EQDAHIPLSDVEKIRAAHPQGVFHVYPADHGFNCNDRASYDAPSARLALERTLAFFAE
                     HVG"
     gene            13776..15230
                     /gene="gabD"
                     /locus_tag="PKN91_04260"
     CDS             13776..15230
                     /gene="gabD"
                     /locus_tag="PKN91_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNS27318.1"
                     /translation="MKLNDPTLLRQQCYIDGEWSDADSRRTLEVTNPASGERLGSIPD
                     AGSAETRRAIEAAARAFPAWAARTAKDRAGILRRWSELMLAHQDDLATLMTAEQGKPF
                     AESKGEIAYAASFIEWFGEEGKRIYGDVIPGHQPDKRILVLRQPVGVVAAITPWNFPS
                     AMITRKAGPALAAGCTFVCKPATQTPYSALALAEIAARAGVPAGVFNVVTGSATAIGG
                     EMTSHPVVRKITFTGSTEIGKKLMVQSAGTLKKLSLELGGNAPFIVFDDADLDAAVQG
                     AIASKYRNTGQTCVCANRLLVQAGVYEAFTAKLSEAVAGLRIGDGLKGATDQGPLIDT
                     KALAKVEEHISDALAHGARIAHGGKRHALGGTFFEPTILTGVTPQMMIAREETFGPVA
                     PLFRFETEAEALRMANDTEFGLAAYFYTRDLARSWRVQEQLEYGIVGVNTGIISTEVA
                     PFGGMKESGFGREGSKYGILDYTELKYVCVGGVA"
     gene            15245..16324
                     /gene="hemH"
                     /locus_tag="PKN91_04265"
     CDS             15245..16324
                     /gene="hemH"
                     /locus_tag="PKN91_04265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNS27319.1"
                     /translation="MPQYHGTLEPDHDRSARVGVLLVNSGSPDSASVRDIRRFLGRLL
                     SDPRVVELPRWLWLPILHGIILPIRPLRSARKYRAIFAAGRSPLLRLSHELSAALEAE
                     LATRPVPPVSVAIGMMYSKPSVRDAITSLTDAGARRLLVVPLFAQYSGVTTAAACDKV
                     ASALAALRWVPELRYVADFHDHPDYIEALRASIAAHWAAHGRSGHLLLSWHGIPSAYC
                     EKGDPYLRRCHATAQLLADALKLREGEWSLAFQSRFGGGRWLRPYTDEVLDALPARGV
                     RTVTVACPGFAVDCLETLEEIDVTDRARFLAAGGKRFDYVPALNARPEHVLALANLVQ
                     AHVRGWGGEGAVRGAAGFPRVVSIV"
     gene            16338..17051
                     /locus_tag="PKN91_04270"
     CDS             16338..17051
                     /locus_tag="PKN91_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27320.1"
                     /translation="MLGRFLELSLMTSDIRASVEFYERLGFTHCATGDTWTHPYGVLS
                     DGRIAIGLHQFRFPSPSLTFVHHDIARRVAQVEAAGVEIAFRRTADDVFNEFGFLGPA
                     GHMVTVLEARTFSPVNRDPAETTLCGEFREYSLPAPDFEIARQRWEAFGFVAIEDEDM
                     PWRRISLTSDHLNLAFHSPRFFEQPLLVFSGADPVGRRERLAALGVAPAHDLPATLDD
                     KRYTLLESPEGLAILLAPE"
     gene            17175..19199
                     /locus_tag="PKN91_04275"
     CDS             17175..19199
                     /locus_tag="PKN91_04275"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNS27321.1"
                     /translation="MPALLLAAALPVLADEGMWTFDNFPSATVKERYGAEIDAAWLDR
                     VRRSTIRLANCTASFVSPDGLILTNHHCVRSCLANLSTRETSLEENGFIARQRGDELR
                     CETQVADVLQSMEDITGRVAQAVAGLDASAANEARKQTLTQLETECERDTGLKCQGVT
                     LYQGGQYFLYRYKRYSDVRLVLAPEADIAAFGGDPDNFQYPRWSLDYSLLRAYEDGKP
                     AKTAHYLPIRFEGPKADELVLVPGHPGSTSRLDTRTDLEFARDVVMPNWLLRYSELRG
                     RYIQFGKGGESAARIVEEPLSSIENGIKVRRKLLDTLHEDAMLDAKSAAERDLRARMA
                     GDASFGDPWADIARADAIRRNIYLPYAFLEGAAGFNSATFRYARTLVRAAAERPKENT
                     KRLREYTDTALPRLERQTLAPVPVYPELENLTLSWSLERMREWLGPDHMVVRQLLAAD
                     SPDTLAARLVAGTKLADPAERKRLWEGGQAAIEASDDPMIRLAIAVDAPSRAMRTQYE
                     DEVEAPISTASEKIAAARFKAYGTDVYPDATFTLRLNYGKVAGWKERGEDIAPFTYLA
                     RAFERATGEPPFRIPASWQQARDTLDMDTPFCISTTSDIVGGNSGSALVDAKGRIVGL
                     LFDGNIHSISGSYWFDTEKNRAVAVHPAIIRESLQKVYRADAILRELGVR"
     gene            complement(19177..19755)
                     /locus_tag="PKN91_04280"
     CDS             complement(19177..19755)
                     /locus_tag="PKN91_04280"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNS27322.1"
                     /translation="MQRHRIDPAESLLAIVVRRGGALASMGHNHVVASHDLSGYIDLA
                     AAPAASTLFMRIPVAALAVDEPGLRAGRGADFGGVVPDSARAGTRSNMLGEDLLDAAR
                     YPEIIVRGITLGGGPVNYAAQVELTVRAQRTTIQVPLRLEPAAAGTLRVSARFPVAQS
                     ALGLMPYSVMLGALRVEDMLEIELDLTARRVP"
     gene            19939..21069
                     /locus_tag="PKN91_04285"
     CDS             19939..21069
                     /locus_tag="PKN91_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNS27323.1"
                     /translation="MQFRNSLERWGSVAKFFHWAIVLLVICQFALALLADDLPVGVEK
                     LKLLARHKSIGITILALATLRLLWRAANRTPALPPTMAAWEKFSSKAAHALLYVLLFV
                     VPLAGWTMSSAKNFPVSWFGLVQLPDFVAPNESTYEIAHGIHETLAMTLGIVAVLHLL
                     AALKHHFVNRDAVLKRMWPFARVPVVLITMAIAAVAMPGQSLAAQPPGATLRGSGADS
                     TLAFNFTQAGAGTNGSFKAFTTTLMTGEDGAPASLEVTIDVKSIDTGDKERDGLLRDK
                     DLFDVKRFAEAKFAATRFTREASGRYQAIGKLTIRDVTRDVTLPFALTEMPGGGAPGW
                     LLAGEIVINRLDYGVGQGEWRDTGMVGNAVTVRYSVKLAPVP"
     gene            21071..23233
                     /locus_tag="PKN91_04290"
     CDS             21071..23233
                     /locus_tag="PKN91_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNS27324.1"
                     /translation="MTQVFDTIDACVDATLERVGPRIVLGLPLGIGKPNLLANEFFRR
                     ATGDPTLELTILTALSLTRPRPRNDLERRLLEPIVERVFADYPELEYVQAARRGTLPP
                     NVRVIEFYFEPGAWLGVESAQQDFLSSNYTHIGRDLLARGANVIAQLVAKRTVGAETQ
                     LSLGSNPDILVDLLPDLAAARAAGRDIVLIGAVHPRMPFMFGGACIDAERFDYLIDHP
                     NYDYELFCPPNLPLRTVDHAIALHASALVRDGGTLQIGIGELGDALVYALLLRHQQND
                     AWRAALLALGGTGEDLAPFRDGLYACTEMLADQMLDLMRAGILRRHVYGSLPLMRALA
                     QGRVGERFDGAILEALRDAGAGPQLDAGEFEQLRRYGVFRSDTRFRDGRIRSPDGEWI
                     AADLADGAARRALAAQCLGRELRGGVVAHASFFVGPRGFYAKLREMPERELRQIDMCG
                     VGYVNQLYGTDYELRCLQRRHARFVNTTMMVTLLGAAVSDTLADGRVVSGVGGQYNFV
                     AMAHALPDARAITCVRATRERAGRLTSNIVWNYPQTTIARHLRDIVVTEYGAADLRGA
                     TDSEVIQRLLNIADSRFQDGLLASAQAAGKIARDYRIPPEHRHNSPERLASTFALPRR
                     AGLFSEYPFGTDLASEEIRLAQALTALAADTATTGGRIGTFIAALRATVPADAQQALA
                     RMGLANPRGWRERLARRLVALALQRLSERTAATDPGTP"
     gene            complement(23193..>23639)
                     /locus_tag="PKN91_04295"
     CDS             complement(23193..>23639)
                     /locus_tag="PKN91_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNS27325.1"
                     /translation="WPLAAQLPMTLQPFHLAVPVHDLALAREFYGGLFGCAEGRSAAE
                     WVDFDFFGHQLVAHLDPGARERRRHHNPVDGHDVPVPHFGVVLDWEAWHELAGRLRAA
                     GTRFVIEPGIRFQGLVGEQATMFLYDPSGNALEFKAFRDPSQLFAR"
BASE COUNT         3737 a         8291 c         7943 g         3668 t
ORIGIN      
        1 acgcgaactc gacgacacga ccgtcggcat cgcctacctc gacgcgatct gctcgggcag
       61 ttcggcgagc ctcaccgagg cgcgattcgg caaactgagc tcggtgctcg tgtcgctggt
      121 cgccgcccac gaaatcggcc acaacttcgg cgcgccgcac gacggcgaat ccggcaaggc
      181 ctgcgcgagc accccgacga ccttcctgat ggcgcccacc ctgtccacgc agaactcgga
      241 gttctcgcag tgcagcctca cgcagatttc ccagcgcatc gcgagcgccg cctgcctcgt
      301 cagcgtggac caggcggacg tggagatcgt ggcgccggtc agcacggtgc gccacgggcc
      361 gggtgagaac tactcgctca ctttcaccgt ccgctccatc ggcacgcgca cggccgagct
      421 cgtgtcggcg acgttcgtgg ttccaccggg cagctcgctc gatgcgatca acgccgcggg
      481 cggcacctgc acgaatttcg gcgcgcaggg caactgctca ctcgggtcgc tggcgcccgg
      541 cacgaaccgc acggtgacgc tgtcgctcac cggccacgcc atcgggaccg aaacgctcaa
      601 cgccgtcgtg ttttcgtcca atgacgaggc gccgggcaac aacagcgtct cggtggaggt
      661 cgtcaccgac ccgatcgccg acatccgtgt gagcctcacc gccactccgg ccagcctcgt
      721 ggttggcgac gacacgaccg tcacggtgac cctggccaac ctcggtccgg cggccgccag
      781 cgatgcgcgg ctgtccgtcg acgtgccggc cggcctcacc atcggcgcga tcaacgcagg
      841 cggactcgac tgcggcgcgg gcgccaacag cgtgtcctgc aatccggtcg cgctcgcggt
      901 ggacgaatcg cgcagcctca cggtgaccct gcgcgccgcc acggccggca cgcggaccgt
      961 cagcgccctc gcgctgtcgt cggccctcga tcccgacagc ggcaacaatt cggcgagcgt
     1021 cgcactgacg atcggcgccg cgggcggcgg cggcgtgccg tcaccgccga gcagtggcgc
     1081 aagtggcggt ggcggcggcg gcgcgacttc gacgctgctg ctcggcctgc tcgcgcttgc
     1141 cctcggcatg cgtgcacggc tcgcaggcgc gcgcgccgcc ggctagccga tgacgaccag
     1201 caggtccttc gcgtcgacct gcatgccggg tcgcgcgagc acttcggcga cctgaccatc
     1261 ctgctcggcc cgcacggccg tttccatctt catcgcctcg agcgtgacga cgacgtcacc
     1321 gcgggcgatc ttctgcccca ccgtaaccgc cacggtggcg acgctgcccg gcataggcgc
     1381 gccgacatgc cgcgtgttgc ccgactcggc cttgcgctgc ggcgggcggc gtgcgacctg
     1441 gctgcgatcg gccacgcgaa tcgcgcgggg ctcgccgttc agctcgaaga acaccgtgcg
     1501 cgtgccgtcg tcgtgcgtct cgctgaccgc gacgtaattg acgatcaggg tcttgccgcg
     1561 ctcgatgtcg atgctgatct cctgccccgg ctccatgcca tagaagaagg cgggagtcgg
     1621 caggcgaccg acgtcgccgt acgccgcgcg atccttcgca tactcgagga acacgcgcgg
     1681 atacatcagg taagaggcca gctggtagtc ggtgatctcg cggccgacct tgcgcgcgac
     1741 ttcggcgcgt gtcgcctcga ggtcaatggg cggcagcacc ttgcccgggc gcacggtgag
     1801 cggctcgatg tccttgagca cctttttctg ctggccctgc ggaaacccgc cgagcggctg
     1861 gccgaggtcg ccgcgcatca actgcaccag cgactcgggg aatgcgatct gcgtgtcggg
     1921 gtcctcgacc tgcgcgcggg tgaggccgct cgtgaccatc aggatcgcca tgtcgccgac
     1981 caccttcgag gtcggcgtga ccttgatgat gtcgcccagc atgtcgttca cgtcggcata
     2041 ggcctgtgcg acatccggcc agcgcgcctc gtcgacgccg agtgcgcgcg cctgttcgcg
     2101 caggttggtg tactgcccgc ccggcatgcc gtgcacgtag acctcggacg ccccggagcg
     2161 gatatcgctc tcgaacgccg catagccgcg gcgcacctgc tcccagtagg aggacaggcg
     2221 gcgcagctgc gcggtgtcga tgcccggatc gcgctcgccg tggcgcagcg cctcggcgat
     2281 cgaaccgagg ttcggctggg aggtgagccc gctcatcgcg tcgatcgccc cgtcgatggc
     2341 gtcgcagccc gcctccgccg cggccagcac gctcgcggcc gcgatgccgc tcgtatcgtg
     2401 cgtgtggaag tgcagcggca ggcccgtttc cgacttgatc gcggtgacga gcgcgcgcgc
     2461 cgccgccggc cggcacaggc ccgccatgtc cttgatcgca atgatgtgcg cgcccgcggc
     2521 cttcagctcg cgagccagcg acacgtagta atcgagcgtg tacttcttct cgcgcggatc
     2581 cgagagattg ccggtgtagc agatcgccgc ctcgcacagt cgcccggtct cgagcacggc
     2641 gtccatcgcg acgcgcatat tctcgaccca gttgagcgaa tcgaacacgc ggaagagatc
     2701 gacgccgcag gccgccgcct gcctgacgaa gtaacgcacg acattgtcgg gatagttcgt
     2761 gtagccgacg gcgttcgacg cgcgcaacag catctgcagc agcaggttcg gcatgccctc
     2821 gcggaactgc gcgagccgtt cccacgggtc ttccttcagg aagcgcatcg cgacatcgaa
     2881 ggtcgccccg ccccagcatt ccaccgacag cagctccggc agcatgcgcg cgtagtacgg
     2941 cgcgatcgcc gccatgtcga tcgtgcgcat gcgggtcgcg agcagcgact ggtgcgcatc
     3001 gcgcatcgtg gtgtcggtca gcagcacgcg ccgctcggcc agcatccacc gggcgaactt
     3061 ctccgggccg agctcatcga gcttctgctt ggcgcctgcc ggcggatccg ccttcagcga
     3121 caccttcggc aggcgcgcga ggcccatgcg ggccggacgc gggcgcccct tcgtttcggc
     3181 gttgccgttg accaccgtct cggcgaggta ggtgagcatg cgcgtcgcgc gatcgcgttt
     3241 gcgcgggaag cggaacagct ccggcgtcga atcgatgaag cgcgtggtgt actcgccgct
     3301 cgcgaacgcc ggatgcgtga tcacctggtc gaggaagcgc aggttggtca ccacgccgcg
     3361 gatgcggaac tcccacagcg cgcggtgcat gcgcgcgctc gcctcttcgg cggtctgcgc
     3421 ccatgcggtg atcttcacca gcagcgaatc gtacgagcgc gtgatgatcg caccggcgta
     3481 ggcggtgccg gcgtcgaggc gaatgccgaa gccggcggga ctgcggtagg cggtgacccg
     3541 gccgtagtcc gggatgaaat tgttctcggg gtcctcggtc gtgacgcggc actgcagcgc
     3601 atgcgcctgc atgcggatct cgtgctgcgg cggcacgccg ctctccggtg tgccgatgcg
     3661 tgcgccggcg gcaatgcgga tctgcgcctt cacgatatcg atgccgaccg cgcattcggt
     3721 cacggtgtgt tcgacctgga tgcgcggatt gacctcgatg aaatagaact cgccggtgtc
     3781 ggcgtcctgc aggaactcga cggtgccggc gttgaggtag ttcaccgcgc ggccgagctt
     3841 caggccggcc tcgcacagtt gcgtgcgctg cgcgtcggtg agaaatacgg ccggcgcgcg
     3901 ctcgaccact ttctggttgc gccgctgcac cgtgcagtcg cgctcgaaca ggtgcacgag
     3961 attgccgtag cggtcgccga ggatctgcac ttcgaggtgg cgcgcgcgac gcaccagctt
     4021 ctcgagatac acctcgtcgt tgccgaacgc cgcctgcgcc tcgcgccgcg ccaccggcag
     4081 cagctcggcg agctgggcgt cattttcgat cacgcgcatg ccacgcccgc cgccacccca
     4141 gctcgccttc agcatcaccg ggtagccaat ctcgcgtgcg agggtctgcg cggcggcggg
     4201 gtcgggcggc agcgccggcg tcgccggcat caccggcacc cccacggagg ccgcggcatt
     4261 gcgcgccgaa accttgttgc cgagcgtgcg catgatctgc ggcgtcgggc cgacgaagat
     4321 cacgccatcg cgctcgcagg cttccgcgaa ctgcggattc tcggacagga acccgtagcc
     4381 cgggtggatc gcgtcggcct gcgcctcgcg cgcgatgcgc agtacatcct cgatgtcgag
     4441 ataggcgtcg acgggaccct tgccgcgacc gacgagataa gcctcgtcgg ccttggtgcg
     4501 atggagcgag aagcggtctt cctgcgaata gatcgcgatc gtgcggatgc cgagttcggc
     4561 ggcggcccgc atcacgcgta tagcgatctc gcctcgattc gcgaccagca ggctgcgaat
     4621 cccggaaacc tggggtgtaa ccactggcaa ctgcacccga tctggttggg ggaactgcgc
     4681 ccgcggcgct ggcggagagc caggattcgg gctgtccgga tgatagctgc cgccgccgcc
     4741 cggtgtcgcc ccttcggcgt aggcaattac cccaaggatg tggcatcgtt ccggccgcaa
     4801 agtgccctaa aaatgaagcg acccgccctc attgtcctgt tgctcgcgac ctgcggcgcc
     4861 ctggctgcac acgcgggaga accacccatg catgctattg ccattcacgg cggcgccggc
     4921 acgctgccgc gcgccgagat gactcccgag cgcgaagccg cctatcgtgg cggcctcgct
     4981 gccgcactcg acgcgggcta cgcggtgctc gacgcgggcg gcaccagcct cgatgcggtc
     5041 atcgccgccg tgcgcattct cgaggacgac ccgctgttca acgccggacg gggggccgtg
     5101 ctgacccgcg aggggatcgc cgagctcgac gcctccatca tgaacggggc gagcctggcc
     5161 gcgggcgccg ttgccggcgt gcggcacgtg aagaacccga tcgagctggc gcgtgccgtc
     5221 atggagcatt ccgcacacgt catgctgatc ggcgccggcg ccgaggagtt cgcgctcgcc
     5281 cgcggcctca cgctcgtgcc gaacgagtac ttccgcaccg agacccgcaa ggcgcagttg
     5341 cagcgcgtgc tcaccggcga cacgcgcccg gcgaacgagc tctcggcgct tggcacggtc
     5401 ggcgccgtgg cgctcgatcg cgccggcaat ctcgccgcgg ccacctcgac cggcggcatg
     5461 acggccaagc gctatggccg cgtgggcgac tcgccgatca tcggtgcggg cacctatgcc
     5521 gacaaccgca gctgtgcggt ctcggcgacc gggcacggcg agttcttcat ccgcgcggcc
     5581 gtcgctcacg acatctgcgc gcgcgtcgcc tacggcggca ggagcctcgc ggaggccgcc
     5641 gacgccgtgg tcatggaccg gctggtgacg ctcggcggcg aaggcggcgt gatcgccatc
     5701 gatccgcagg gccgcatcgc gatgcccttc aacagcgagg gcatgttccg tggcagccgc
     5761 agcgccggcg gcgagcgggt gatcgcgatc taccgcgacc gctaggcccg cagttcgcgc
     5821 gcggcaagca ccacggtcgc ctcgccgaga tccgcctccc cgagcacggc gtgccggttg
     5881 cgcccctgct ccttggcgag atagagcgca cggtcggcga tgcgtacgat caggtcgtgc
     5941 gaggaatcgc gcccgggcag catcgtcgcc acgccgatgc tcacgctgac catcggtgcg
     6001 gtcagcgatc gatcgtgcgg gatcgcgagc gccgcgacct cctcgagcat cgcgcgcgcc
     6061 acctgttcgc cgccggcgcg atccgcgccg ggcaggatcg cgatcatctc ctcgccgccg
     6121 tagcgcgcca cgcaatcgcg cgcacgggta ccggccgcgc gacgaatcgc ccccgcgacc
     6181 gcgcgcaggc aggcgtcgcc gccctggtgc ccgtagcgat cgttgtaagc cttgaagtga
     6241 tcgatatcga tcatcaggac ggtgacgtgc tcgcgatcgc ggatcgcctg ctgccagatg
     6301 cgatggatct cgctctcgag agccgagcgg ttcagcagct gcgtgagccc gtcatgcgtc
     6361 gccacttcgc gcagcatgcg gccttcgagg aaggagaccc ggttggcgtg ctcgagcgcg
     6421 tagctgccgg caccggcata caggttggcg aacagcagca ggaagaactg gtagatcgaa
     6481 gtctgcggcg caatgtcgcc gacgaatgcc gcaacgacga agcccgcgag cgtcagcagg
     6541 ttggtgcgca gcgccgcctt gaacgacagg ccgagcatga aatagaagaa gaatatcgcg
     6601 agcacgagcc gcgcgtggat gaaggccgtg agcggctccg gggcgttggc ggccagcagc
     6661 accgtggcga ggccgtagag cggcgcgaat acgtggacga atggccagta ccagcggaag
     6721 tagcgccgcg aggtgagcac gataccggtg acgacgagcg gaatgtgcac accgaagcgg
     6781 atcgcatcgg ggacgacctg cgcgccgggg ccgagcaacc agcgatcgag gatcgcgagc
     6841 ccgatgatcg tcgtgagcgc gaggcataca ccgatgcgca cgatgcgccg gcgcgaagtg
     6901 cggtactcgg caacgaactc acgctcgagt tcgcccggga acctgagcca tggaaatccc
     6961 gcgcgcagcg tgcgggctac gggggactcg gactcctccg gcattgctgc cgtgggcatt
     7021 ggtcaggcga cgggattgtc gctctcgcgc ggaatcttca cccgcgtgcg ccacacgtcg
     7081 tagggatccc agccgcgtgt cggcgtcgcc tcgtgggcgc gttccctgcc cgggacgttg
     7141 gagatcgtcg gctcgttggc cgcgacgagc gcggcggaag cgtgtccgtc agcctcgaaa
     7201 ggcttgctgg tgttcatctg cggggtcgtc cctgatttcg ggctgtctgc gtaaggcgac
     7261 ggattctaac gcctgcagcc tgacaagggc acaacaaaaa tgctcttcgg atatgtcaaa
     7321 tcagcggttt gcttccagtt ctgtgagcta gttctcaaaa taacggcgca ggtagtcttc
     7381 gaagctgccg gcgtccgccg actcgatctt ctgctgcgct gcaacagaat cctcggcttc
     7441 acgctggaac tccgccaggc gctccgcatt gggagggtgc agctcgagga aataggactt
     7501 gtgggcgctc gacatctgct gcgccacgtc gaagaacgac tcgccggtcg tgcggatctc
     7561 gtgcagcagc cgggcggacg gggtcagcgc gggatcgcgc agcttgtcgc gctgcgcggc
     7621 gagcgccgcc gaatagggtc gtgccgcatg gccgtcgtcg aggatctcgc acaggccggt
     7681 catgccgtcg agcaattctt cggcccagtc cgcgagaggc acggcgcgcc cgtcccgctg
     7741 caaccgcagg cccggctcgc gtccgcggcg cgcggcgacg acatggttca tgtcgagcag
     7801 ctcctgttca tcgttgccga tcggcgggct gtcgcgcagc aggcacagcg cgaggaaggc
     7861 ctcgaggaaa cgcagattca cctgattcac gccaaccggg tcgaaggcac tcacgtcgag
     7921 agcgcgcact tcgacgtatt cgacgccacc gcgcaacagc gccctggtcg gccgctcgcc
     7981 cgagcgcgcg acgcgcttcg ggcggatgct gctgtagaac tcgttctcga tctgcagcag
     8041 gttggcgttg agctggcgcc actcgccgcc gaccttgacg ccgatcgcct cgtacggcgg
     8101 aaacggtgtg ctgatcgcgc gcgtgagatc gcgcacgtac tcctcgaggc tgttcaccga
     8161 gaccgaaacg cgcgcctgct tgcggttgcg atagccgaga tcgctcatgc gcagtgtggt
     8221 cgcatacggc tcgtaggccg tgccgtgcgc gagttgcggc aacgtctcgc cgcgcacgcg
     8281 caggaaggaa ttgcacaccg cgggcgaggt gccgaacagg tacagcacga gccagccatg
     8341 gcggcggtaa ttgcgcagca ggtcgaaata ggccgccgag cgcaaggcct tctcgtccgc
     8401 gcactggcgc agcgcggcgt agggttccca gaactgctcc gggaacgaat agttgaaatg
     8461 cacgccggag atcgcctgca tggcgcggcc gtagcggttg ccgaggccgt tgcggtacac
     8521 ggtcttcatc cgcccgacgt gcgaactgcc gtactgcgcg atcgggatcg catcgtcgct
     8581 ctcgatccgg cacggcatcg aggtcgccca cagcagttcg ctgccgatat ggcgatagac
     8641 gaactggtgc atgtcgcaca ggtactgcag cagctcccag ttcgtggtga aagtcggcgt
     8701 gaccagctcg atcagcgcct cggaataatc ggtggtgaac tggccgttgg tgagcgccga
     8761 gccgagcgcg cgcggatgcg gtgtcgtcgc gaggcgcccc tcgggcgtga cgcgcagcga
     8821 ttccttctcc acaccgcgcc ggccgccctg cagcagccgc gcgccgccgc cattgacgag
     8881 cgcggcgacg cggcgctcaa acgtgcgatc gatggcgggc gcggccaccg tgctaccagg
     8941 gcagtcggcg cagcaggatt tcgcgcagca tcgtgaactc gccgcgcaag ctgtacagcc
     9001 gacagcggaa taacgggtag gactcgcgga ggctcgcgca gcgctgcgac atgatccggg
     9061 catcttgccg gttgcccgta ccggctcgca agggcccgca gcgcggcggg caccgcgcct
     9121 cgcaatgccg ggctgccgcc gccgcggtca ttgtccgttc agcatggccg gcgtaccgtg
     9181 accccatgag gacaggcaag ctgttcgcgg cactgctgct gtgcgcatcg ggtttcacgg
     9241 ccgcgcaggc cgacaacatg cgctgtggcc agcgcctcgt ggtcgatggc acgacccgcg
     9301 accaggtggc gacctggtgc ggcgaaccga ccgaggtcct gcagcggaca gtgctgcgcc
     9361 cgccgatcgt ctggtactac ggtcgcccgg tgcacgtcgg cggcggccac atcgaggtgc
     9421 tggtcgagac ctggatctac aacctcggcc cgaacaagct gatgcggcgc gtgatcttcg
     9481 aggacggcct cgtggtcgaa gtcgagacac tcggctacgg ctatcgcggc tcgccgcccg
     9541 cacgcgagaa ctgacgcgca gcggaaaatg gctgaaccgg cgcacagcgt ggcatgctag
     9601 ccgcctatgc tcgcaggacc agccatcagg cgcgtcgcga tcgtcggcgg ccagcgtatc
     9661 cccttcgcgc gggcgcacgg cgcctacgcc acggtcggca accaggacat gctcaccgcg
     9721 gcgctgcgcg ccgtcgtcga gaagtacggg ctcgccggcg tgcgcctcgg cgacgtgatc
     9781 gccggtgcga cgatcaagca ttcgaaggac ttcggcctcg tgcgcgagtg cgtgctgtcg
     9841 agcggactcg acccgcagac acccgggctc gacttgcagc gtgcctgcgg cacgagcctc
     9901 gaggcggcga tactcgtcgc caacaagatc gctctgggcc agatcgacgc cggcatcgcc
     9961 ggcggcgtcg acacggtgag cgacccgccg atcgtctatc cgcgctccta ccagcagaag
    10021 ctgctgcgca gctaccgcgg ccgcagcttc ggccaacgca tcgcgccgtt cttcggcctg
    10081 cggccgcgcg acttcaagcc ggtcatgcct gcggtaatcg aaccacgcac cggcatgtcg
    10141 atgggcgagc actgcgagca gatggcgaag acgtgggcca tcacgcgcga ggcgcaggac
    10201 cagctcgcgt acgaaagcca catgaaggcg gcggcggcct ggaaggacgg cttctatgac
    10261 gatctcgtcg tcgacttcca gggattgaaa accgacaaca acatccgcgc cgacacctcg
    10321 ctcgacaagc tcgcgaagct cgcgccctcc ttcgaccggc aggccggcac gctcaccgcg
    10381 ggcaacagca cgccgctcac cgatggcgcg tccgccgtgc tgcttgcctc ggaggactgg
    10441 gccagatcgc gcaacctgcc ggtgctcgcg tggttcagcc acggcaagac ctcggcggtc
    10501 gattacgtgg gcggccacga aggcctgctg atggcgcccg tgcatgccgt gcccgcgatg
    10561 ctgcgcgacg cgaacctcac cttgcaggat ttcgacttct acgaaatcca cgaagccttc
    10621 gaggcgcagg tgctgtgcac gctggccgcc tgggaatccg ccgactattg ccgcgagcgg
    10681 ctcggcctcg agcagccgct cggcagcatc gatcgcggca gactgaacct gcgcggcggc
    10741 agcgtggcaa tcggccaccc ctttgccgcg accggtaccc ggatcgtcgc ctcgctcgcg
    10801 aagcagctcg cggaggcgcc cggcgcgaag cgcgggctga tttccgtctg cacggcgggc
    10861 ggccagggcg tggccgcggt gctggagcgc agcagcgatt cctgagccga cttgtaaata
    10921 gtttcatttg aaactaaatt gcccggatga accgctacgt cggttttccg cgagtcgagg
    10981 gcagcgcctc ccgccaggcg cacgccgacc tgccagccgg caccttcgag cgcgaaatca
    11041 gcaaggaagg cttcttcggt cccgcagccc acctctatca ccgccacccg ccgaccggct
    11101 gggtgagctt cgagggaccg ctgcgaccac acgccttcga cctcacgcgc ctcgccgctg
    11161 ccgcgccgtc gccatgggat gcgccgccga tactcggcaa tgcctcggtc gaaattcgct
    11221 ggtggcagct cgagggcgcc atgccggcac tggcgcgcaa cgcggacggc gaccagctca
    11281 tcttcatcca ccgcggccat ggcgcgctgt actgcgactt cggccacctc gatttcgagg
    11341 cgggcgacta tctgctgctg ccgcgcggca ccatgtggcg gctcgagtgc gcggcgccgg
    11401 tcgcggcgtt gctggtcgag gccaccaaca cgagctacat gctgcccgat cgcggcctcc
    11461 tcggcccgca cgccatcttc gatccggcga tcctcgacac gccgcgcatc gactccgcct
    11521 tcgaggccca gcagtcgtcg tcgcagccct ggcgtgtcga agtgaaacgc cgtggcgcgc
    11581 gttcggtgat cggctatccg ttcaatccgc tcgacgcggt cggctggcat ggcgatctcg
    11641 cgccagtgcg cctgaacgtg cgcgacatcc gcccgctgat gagccatcgc taccacctgc
    11701 cgccctcggc gcacaccacc ttcgtcgccg ggcgtttcgt cgtgtgcacc ttcgtgccgc
    11761 ggccattcga aaccgatccg ggcgcgatca aggtgccgtt cttccacaat aacgacgact
    11821 acgacgaagt cctcttctac cacgccggcg acttcttcag ccgcgacaac atccgccccg
    11881 gcatgatgac cctgcacccg gcgggcttca cccacgggcc gcatccgaag gcgctcgcca
    11941 acatcgccac gcaggcgaag cccgccaccg acgagttcgc ggtgatgatc gatacgcgtg
    12001 acgcgatcga catcgcgccc gccgcggccg gcgtcgagtg ggccggctac gtcgacagct
    12061 ggaaaggcgc gtgaaactcg cgagccttaa gagcggccgc gacggtcggc tggcggtcgt
    12121 cagccgcgat ctgacgcgct gcgcgctcgc cgatggcatc gccgcgacgc tgcagcaggc
    12181 cctcgacgac tggcagcgca ccgcgccggc gctggctgaa cttgcggtgc gcctcgaggc
    12241 cggcaccgtc gccgccgcga ggcccttcga tccgcgcgac tgcgccgcgc cgctgccgcg
    12301 cgcgcatcac tgggtcgacg gcagcgccta cgtcaaccac gtcgagctcg tgcgcaaggc
    12361 gcgcggcgcg gaactgccgg cgagcttctg gaccgacccg ctgctctacc agggcgggtc
    12421 ggacgacctg ctcggcccga ccgacgacgc cgccttcggt agcgaggagt tcggtatcga
    12481 tctcgaagcc gaggtcgcgg tgatcgtgga cgacgtgccg atgggctgca gcgccgccga
    12541 cgccgcggag cgcatccgcc tgctgctgct cgtcaacgac tggtcgctgc gcaacctcat
    12601 cccgggcgag ctcgccaagg gattcggctt ctaccagtcg aaaccggcca ccgcgttctc
    12661 gccggtcgcg gtcacgcccg acgaactcgg cgaggcgtgg cacgaaggca aactgcacct
    12721 gccgctcgtg agccacgtca acggtgcgcg cctcggcgcg ccggatgccg gcaccgacat
    12781 gaccttcggt ttcccgcagc tgatcgcgca cgccgcgcgc acccgtcgcc tcggcgctgg
    12841 cgccattgtc ggctccggca ccgtctcgaa ctacgaccgc agccgtggct cgagctgcct
    12901 cgccgagaag cgcatgctcg agcagatcgc gcgcggcgag gcgcagacgc cgttcctgcg
    12961 ctacggcgac cgcgtgcgga tcgagatgtt cgatgccgcg ggcgcgtcca ttttcggcgc
    13021 catcgaccag cgcgtggttc ccgccgcctg accgctgtat tcttgtcggc ccccgatccg
    13081 cttgagggca tgccgatggg cgagtacgtg acgctgatgg cccgcgacgg ccacgagttc
    13141 caggcctggc tcgccgcgcc caagggtacg ccgcgcggcg ccgtggtggt ggtacaggaa
    13201 atcttcggtg tgaatcgcca catccgcgcc gtcacggatg cctatgcggc ccagggctac
    13261 accgcaatcg cgccggcgct gttcgaccgc gtgcgccgcg gcatcgaact cggctacacg
    13321 gccgacacca tgcaggaagg catcggctac atgatgcaga tgaagccgga gcatttcctc
    13381 gccgacgtca gcgccgcgat caacgcggtc aagagctcgg gccgggtcgg catcgttggc
    13441 tactgctggg gcggcaccgt ggcctggctc gcggcctgcg aactgccggt ggccgcggcc
    13501 gcgtcgtact acggcggtgg catcgtgaaa atgctcgacc gcaagccggc gcgtccggtg
    13561 atctaccact tcggcgagca ggacgcacac attccgctca gcgacgtcga gaagatcagg
    13621 gccgcgcatc cgcaaggcgt gttccatgtc tatccggccg accacggctt caactgcaac
    13681 gaccgcgcgt cgtacgacgc gccgagcgcc aggctcgcgc tcgagcgcac gctggcattc
    13741 ttcgccgagc acgtcggctg aagcctggag aatccgtgaa actcaatgac ccgaccctgc
    13801 tgcgccagca gtgctacata gacggcgagt ggagcgacgc ggattcgcgg cgcacgctcg
    13861 aagtgacgaa tcccgcctcg ggcgagcggc tcggctcgat ccccgacgcg ggcagtgccg
    13921 agacgcgccg cgccatcgag gccgccgcgc gggcgtttcc ggcctgggcc gcgcgcaccg
    13981 cgaaggaccg cgcgggcatc ctgcgtcgct ggagcgagct gatgctcgcg caccaggacg
    14041 acctcgcaac actgatgacc gccgagcagg gcaagccgtt cgcggagtcg aagggcgaga
    14101 tcgcctatgc agcgtcgttc atcgaatggt tcggcgagga aggcaagcgc atctacggcg
    14161 acgtgatccc gggccaccag cccgacaagc ggatcctcgt gctgcgccag ccggtcggcg
    14221 tggtcgccgc gatcacgcca tggaatttcc cctcggcgat gatcacgcgc aaggcgggcc
    14281 ccgcgctcgc cgcgggctgc acgttcgtct gcaagccggc cacccagacg ccctactcgg
    14341 cactcgcgct cgcggagatt gccgcgcgcg ccggcgtgcc ggccggcgta ttcaacgtgg
    14401 ttaccggcag tgcgaccgcg atcggcggcg agatgacctc gcatcccgtg gtacgcaaga
    14461 tcaccttcac cggctcgacg gagatcggca agaagctgat ggtgcagtcc gccggtaccc
    14521 tgaagaagct ctcgctcgaa ctcggcggca atgcgccgtt catcgtcttc gatgacgccg
    14581 acctcgatgc cgccgtgcag ggtgcgatcg cgagcaagta ccgcaatacc ggccagacct
    14641 gcgtatgcgc gaatcgtctg ctggtgcagg cgggtgtcta cgaggcattc accgccaagc
    14701 tcagcgaagc cgtcgccggc ctgcggatcg gcgacgggct gaagggcgcc acggaccagg
    14761 gcccgctgat cgataccaag gccctcgcca aggtcgagga gcacatcagc gatgcgctcg
    14821 cgcacggcgc caggatcgcc catggcggca agcgccatgc gctcggcggc acgttcttcg
    14881 agcccaccat cctcaccggc gtcacgccgc agatgatgat cgcgcgcgag gaaaccttcg
    14941 ggccggtcgc gccgctgttc cgcttcgaga cggaggccga ggcattgcgg atggccaacg
    15001 acaccgagtt cgggctagcc gcatacttct atacacgcga tctcgcgcgc tcatggcgtg
    15061 tgcaggaaca actcgagtac ggcatcgtcg gcgtcaatac cggcatcatc tcgaccgagg
    15121 tggcgccgtt cggcggcatg aaggaatcgg gcttcggccg cgagggctcc aagtacggca
    15181 tactcgacta caccgaactc aagtacgtgt gtgtcggcgg cgtcgcctga cgcgcgggaa
    15241 ttgcatgccg cagtatcacg gcaccctgga gcccgatcac gaccgcagcg cgcgcgttgg
    15301 cgtgctgctg gtcaattccg gatcgccgga ttcggcgtcg gtgcgcgaca tccgccgctt
    15361 cctcggcaga ctgctgtccg atccgcgcgt ggtcgagctg ccgcgctggc tgtggctgcc
    15421 gattctgcac ggcatcatcc tgccgatccg accgctgcgc agcgcgcgca agtaccgcgc
    15481 gatcttcgcc gccggccgct cgccgctgct gcggctgtcg cacgagctga gcgcggcgct
    15541 cgaagccgag ctggcgacgc gcccggtacc accggtgtcg gtggcgatcg gcatgatgta
    15601 ctcgaagccg tcggttcgcg atgccatcac cagtctgacc gacgccggcg cgcggcgcct
    15661 gctcgtcgtc ccgttgtttg cgcagtactc cggcgtcacc actgcggcgg cgtgcgacaa
    15721 ggtcgccagt gcgctcgctg cgctgcgctg ggtccccgaa ctgcgctacg tcgccgactt
    15781 ccacgaccac cccgactaca tcgaggcgct gcgagcgagc atcgcggcac actgggccgc
    15841 gcatggccgc agcggccacc tgctgctgtc ctggcacggc attccgagcg cgtactgcga
    15901 aaaaggcgac ccctatctgc gacgctgtca tgcgaccgcg caactgctgg ccgatgcgct
    15961 caagctgcgc gagggcgaat ggagcctggc gttccagtcg cgcttcggtg gcggcaggtg
    16021 gctgcggccc tacaccgacg aagtgctcga tgcgctgccg gcgcgcggcg tgcgcacggt
    16081 gaccgttgcc tgcccgggct ttgcggtcga ctgcctcgaa acgctcgagg aaatcgacgt
    16141 gaccgaccgg gcccgcttcc ttgccgccgg cggcaagcgc ttcgactacg tgccggcgct
    16201 caacgcgcgc ccggagcatg ttctcgcgct cgccaacctg gtccaggccc atgtacgggg
    16261 ctggggcggc gaaggggcgg tgcgtggcgc cgccgggttc ccgcgcgtcg tctcgatcgt
    16321 ctgaggcggg cggcgcgatg ctcggccggt tcctcgaact gtcgctgatg acgtccgaca
    16381 ttcgcgcctc ggtcgagttc tacgagcggc tcggcttcac gcactgcgcg accggcgaca
    16441 cctggaccca tccgtatggc gtgctgagcg acgggcgcat cgcgatcggc ctgcaccagt
    16501 tccgctttcc ttcgccttcg ctgaccttcg ttcaccatga catcgcgcgg cgcgtggctc
    16561 aggtcgaggc cgccggcgtc gagatcgcat tccgccgcac ggccgatgac gtattcaacg
    16621 aattcggctt cctcggtccc gccggccaca tggtcacggt gctcgaggcg cgcactttct
    16681 cgcccgtgaa tcgcgatccc gccgagacca cgctctgcgg cgaattccgc gagtacagcc
    16741 tgccggcccc cgacttcgaa atcgcacggc agcgatggga ggcgttcggt ttcgtcgcga
    16801 tagaagacga ggacatgccc tggcggcgca tttccctgac gagcgaccac ctgaacctcg
    16861 cgttccattc accgcgcttc ttcgagcagc cgctgctggt gttctccggc gcggaccccg
    16921 tcggccggcg cgaacgcctg gccgcgctcg gcgtcgcccc cgcccacgat ctgcccgcaa
    16981 cgctcgacga caagcgctac accctgctgg agagtcccga ggggctggca atcctgctgg
    17041 cacccgagta acgcatcccg cgcgtcgctt cgtcgcattg gcgggggccg agagtgtccg
    17101 ccgcgcgccc gggaccccgc gcggcagcta gaatcccgcc atccgttttt cgtgaggcca
    17161 tcatgcgcaa ggtcatgccc gctctgctgc tcgccgccgc cctgcccgtc ctcgccgacg
    17221 aaggcatgtg gactttcgac aactttccgt ccgccacggt caaggaacga tacggcgcgg
    17281 agatcgacgc ggcatggctc gatcgcgtcc gtcgcagcac gatccggctc gcgaactgca
    17341 ccgcctcgtt cgtctcgccc gacggcctca tcctcaccaa ccatcactgc gtgcgaagct
    17401 gccttgccaa cctgtcgacg cgggagacga gcctcgagga gaacggcttc atcgcgcgcc
    17461 agcgcggcga cgagctgcgc tgcgaaaccc aggtggccga cgttctgcaa tcgatggagg
    17521 acatcaccgg cagagtcgcg caggccgtcg ccggactcga tgccagcgca gcaaacgagg
    17581 cccgcaagca gacgctcacg cagctcgaaa ccgaatgcga gcgtgacacc gggctcaagt
    17641 gccagggggt gactctctac cagggcggcc agtatttcct ctatcgctac aagcgctact
    17701 ccgacgtgcg actggtactc gcgccggaag ccgacatcgc cgcattcggc ggcgaccccg
    17761 acaacttcca gtatccgcgc tggtcgctcg actactcgct gctgcgcgcc tacgaggatg
    17821 gcaagccggc gaaaaccgcg cactacctgc cgatccggtt cgagggtccg aaggcggacg
    17881 agctggtgct ggtgccgggc cacccgggct ccacatcgcg gctcgacacg cgtaccgacc
    17941 tcgagttcgc gcgtgacgtg gtgatgccga actggctgct gcgctactcc gagctgcgtg
    18001 gccgctacat ccagttcggc aagggcggcg agagcgccgc acgcatcgtc gaggagccgc
    18061 tcagcagcat cgagaacggc atcaaggtgc gccgcaagct gctcgatacg ctgcatgagg
    18121 atgcgatgct cgacgcgaag agcgccgcgg agcgcgacct gcgcgcgagg atggcgggcg
    18181 atgcctcgtt cggcgacccg tgggccgaca tcgcgcgcgc ggatgcgatc cggcgcaaca
    18241 tctacctgcc gtacgcattc ctcgaaggcg ccgcgggatt caacagcgca acgttccgct
    18301 atgcacgcac gctggtgcgc gccgccgccg agcgcccgaa ggagaacacc aaacgcctgc
    18361 gagagtacac cgatacggcg ctgccgcgac tcgagcgcca gacactggca cccgtgccgg
    18421 tctatcccga gctcgagaac ctcacgctgt cgtggtcgct cgagcgtatg cgcgaatggc
    18481 tgggcccgga ccacatggtc gtgaggcagc tgctcgcggc ggattcaccg gacacgctcg
    18541 cggcgcgact ggtcgcgggc acgaagctcg ccgatccagc cgagcgcaag cgcctgtggg
    18601 aaggcggcca ggcggcgatc gaggcctcgg acgatccgat gatcaggctc gcgatcgcgg
    18661 tcgatgcgcc ctcgcgcgcg atgcgcacgc agtatgaaga cgaagtcgag gcgccgatct
    18721 ccaccgcgtc ggagaagatc gccgccgcgc gcttcaaggc ctacggcacc gacgtgtatc
    18781 ccgatgccac cttcacgctg cgcctcaact acggcaaggt agcgggctgg aaggagcgcg
    18841 gtgaggacat cgcgccgttc acctacctcg cgcgggcttt cgagcgtgcg accggcgagc
    18901 cgccgttccg catcccggcg agctggcagc aggcgcgcga cacgctcgac atggatacgc
    18961 cgttctgcat ttcgaccacc agcgacatcg tcggcggcaa ttcgggtagc gcactggtcg
    19021 atgcaaaagg acgcatcgtc ggcctgctgt tcgacggcaa catccactcg atatcgggca
    19081 gctactggtt cgacaccgag aagaaccgcg cggtcgccgt ccatccggcg atcatccgcg
    19141 agtcgctgca gaaggtgtac cgcgccgacg cgatactcag ggaactcggc gtgcggtgag
    19201 atcgagctcg atctcgagca tgtcctcgac ccggagtgcg ccgagcatca cgctgtaagg
    19261 catcaggccg agcgcactct gggccacggg aaacctggca ctcacgcgta atgtgcccgc
    19321 ggcagcgggt tcgaggcgca agggcacctg gatcgtggtg cgctgcgcgc gcacggtgag
    19381 ctcgacttgc gcggcgtagt tcacgggtcc gccgccgagc gtgatgccgc gcacgatgat
    19441 ctccggataa cgcgccgcat ccagcaggtc ttcgcccagc atgttgctgc gcgtccccgc
    19501 gcgcgcgctg tccggcacca cgcccccgaa gtcggcgccg cgtccggcgc gcaatccggg
    19561 ctcatcgacc gccagcgcag caaccggtat gcgcatgaag agtgtgctcg ccgcgggcgc
    19621 cgccgcgagg tcgatgtagc ccgacaggtc atgcgaggcg acgacgtggt tgtgccccat
    19681 cgaggcgagc gcaccgccac gccggaccac gatcgcgagc agtgattcgg ccggatcgat
    19741 gcggtgacgc tgcatcccgg gcgccggggg cgcctccgcc gccagtgagg gatcgtgcga
    19801 cgcaggcact gccggctcgg gcccggcagg tccacgcccc gcgctcgggc atccggccag
    19861 gcacagcgcc gcgacgatcg ccgccgcacg agccgccttg ctagacttgg agtcccttcc
    19921 gcccacggag cagagcttat gcaattccgt aattccctgg aacgctgggg ttcggttgcc
    19981 aagttcttcc actgggcgat cgtgctgctc gtgatctgcc agttcgcgct ggccctgctg
    20041 gcggacgacc tgcccgtcgg cgtagagaag ctcaaactgc tggcgcgtca caagtcgatc
    20101 ggcatcacga tcctggcgct cgccacgctg cgcctgctgt ggcgcgcggc caatcgcacg
    20161 ccggcactcc cgccgaccat ggcggcctgg gagaaattct cgtccaaggc ggcgcacgcg
    20221 ctgctgtacg tgttgctgtt cgtcgtgccg ctcgccggct ggacgatgtc gtcggcgaag
    20281 aatttcccgg tttcctggtt cgggctcgtg cagctgccgg acttcgtcgc accgaacgaa
    20341 tccacctacg aaatcgcgca cggaatccac gagactctcg ccatgaccct cggcatcgtc
    20401 gcggtcctgc acctgctggc cgcgctcaag caccacttcg tcaaccgtga cgccgtgctg
    20461 aagcgcatgt ggccgttcgc gcgcgtgccg gtggtgctga tcacgatggc tatcgccgcc
    20521 gtcgcgatgc cgggacagtc gctggcggcg cagccccccg gcgccacgct ccgcggtagc
    20581 ggcgccgaca gcacactcgc cttcaacttc acgcaggcgg gggccggcac gaacggctcg
    20641 ttcaaggcgt tcacgacgac tctgatgacg ggtgaggacg gcgcgcccgc gtcgctcgag
    20701 gtgaccatcg acgtgaagtc catcgacacc ggggacaagg aacgcgatgg gctgctgcgt
    20761 gacaaggacc tcttcgacgt caagcgtttt gccgaggcga agttcgccgc gacgcgcttc
    20821 acgcgcgaag cgagcgggcg ctaccaggcg atcggcaagc tgacgatccg cgatgtcacg
    20881 cgcgatgtca cgctgccgtt tgcgctcacc gaaatgccgg gcggtggcgc gccgggctgg
    20941 ctgctcgccg gcgagatcgt catcaaccgc ctcgactacg gcgtcggcca gggcgagtgg
    21001 cgggacaccg ggatggtcgg caacgcggtt acggtcaggt actcggtcaa gctcgctccc
    21061 gtgccctgaa ttgacgcagg tcttcgacac gatcgacgcc tgcgtcgacg ccacgctcga
    21121 gcgcgtcggc ccgcgcatcg tgctcggcct gccgctcggc atcggcaagc cgaacctgct
    21181 cgcgaacgaa ttcttccgtc gcgcgaccgg cgacccgacc ctcgagctga cgatcctcac
    21241 ggcgctgtcg ctcacccggc cgcgcccgcg caacgatctc gaacgccgcc tgctcgagcc
    21301 gatcgtcgag cgcgtattcg ccgattaccc ggagctcgaa tacgtgcagg cggcaaggcg
    21361 cggcacgctg ccccccaatg tcagggtgat cgagttctac ttcgaacccg gcgcctggct
    21421 cggggtcgaa tccgcccagc aggatttcct ctcgtcgaac tacacgcaca tcgggcgcga
    21481 tctgctcgcg cgcggcgcca atgtgatcgc gcagctggtc gcaaagcgca cggtcggcgc
    21541 cgagacgcag ctctcgctcg gctccaatcc cgacattctc gtcgacctgt tgcctgatct
    21601 cgcggccgca cgcgccgcgg ggcgcgacat cgtgctgatc ggcgccgtgc acccgcgcat
    21661 gccgttcatg ttcggcggcg cgtgcatcga tgcggaacgc ttcgactacc tgatcgacca
    21721 tccgaattac gactacgagc tcttctgtcc gccgaacctg ccgctgcgca ccgtggacca
    21781 tgccatcgcg ctgcatgcga gcgcgctggt gcgggacggc gggacgctgc agatcggcat
    21841 cggcgaactc ggcgacgcgc tggtgtacgc actgctgctg cgccaccagc agaacgacgc
    21901 atggcgcgcc gcgctgctgg cgctcggtgg caccggcgag gatctcgcgc cgtttcgcga
    21961 cggcctgtat gcctgcaccg aaatgctggc ggaccagatg ctcgacctga tgcgcgccgg
    22021 gatcctgcgc cgccacgtgt acggctcgct gccgctgatg cgtgcgctgg cgcaggggcg
    22081 cgtcggcgag cgcttcgatg gcgccatcct cgaggcgctg cgcgatgccg gcgccggacc
    22141 gcagctcgat gccggcgagt tcgagcagct gcgccgctac ggggtgttcc gcagcgacac
    22201 gcgttttcgc gacgggcgca ttcgcagccc cgacggcgag tggatcgccg cggatctcgc
    22261 cgacggtgcc gcgcgccgcg cgctcgcggc gcagtgcctc gggcgggaac tgcgcggcgg
    22321 cgtggtcgcg catgcgtcgt tcttcgtcgg cccgcgcggc ttctacgcca aactgcgcga
    22381 gatgccggag cgcgaactgc gccagatcga catgtgcggc gtcggctacg tcaaccagct
    22441 ctacggcacc gactacgagc tgcgctgcct gcagcgccgg catgcgcgct tcgtcaacac
    22501 gacgatgatg gtcacgctgc tcggtgccgc ggtgtccgac acgctggcgg acggccgcgt
    22561 ggtgagcggc gtcggtggcc agtacaactt cgtcgcgatg gcgcacgccc tgcccgatgc
    22621 gcgggcgatc acctgcgtgc gcgccacgcg cgagagggcc gggcgcctga cctcgaacat
    22681 cgtgtggaac tacccgcaga cgaccattgc ccgccacctg cgtgacatcg tcgtgaccga
    22741 gtacggggcc gcggacctgc gcggcgcaac ggacagtgaa gtcatccagc ggctgctcaa
    22801 catcgcggac tcgcgcttcc aggacggcct gctcgccagc gcgcaggcgg ccggcaagat
    22861 cgcgcgcgac taccggatac cgcccgagca tcggcacaac tccccggaac gccttgcgtc
    22921 gacattcgca ctgccgcgcc gtgcaggcct gttcagcgag tacccgttcg gcaccgacct
    22981 cgcgagcgag gaaatccggc tcgcgcaggc gctcaccgcg ctggccgcgg acaccgcaac
    23041 gacaggcggg cgcatcggca cgttcatcgc ggcgctgcgc gcgaccgtac cggcggacgc
    23101 ccagcaggca ctggcacgca tgggactcgc caatccccgg gggtggcggg agcgactcgc
    23161 gcgccggctc gtcgcgctcg cgttgcagcg actcagcgag cgaacagctg cgacggatcc
    23221 cggaacgcct tgaactcgag cgcgttgccg gaaggatcgt agaggaacat cgtcgcctgc
    23281 tcgccgacca ggccctggaa gcggatccca ggctcgatca cgaaacgcgt gccggctgcc
    23341 cgcaggcgtc ctgcgagttc gtgccacgct tcccaatcga gcaccacgcc gaaatgcggc
    23401 accggtacat cgtggccgtc gaccggattg tgatgcctgc ggcgctcgcg cgccccggga
    23461 tccaggtggg cgacgagctg gtggccgaag aagtcgaagt cgacccactc ggcagcgctg
    23521 cggccctccg cgcaaccgaa cagcccgccg tagaactcgc gggccagcgc caggtcatgt
    23581 accggaacgg cgagatggaa cggctgcagg gtcatgggca gctgcgcagc caacggcca
//
LOCUS       DAOQVY010000028        23292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_2572_length_23292_cov_5.599905, whole genome shotgun sequence.
ACCESSION   DAOQVY010000028 DAOQVY010000000
VERSION     DAOQVY010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 23292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23292
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2572_length_23292_cov_5.599905"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1069)
                     /locus_tag="PKN91_04300"
     CDS             complement(50..1069)
                     /locus_tag="PKN91_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HNS27326.1"
                     /translation="MRQTATRAMRLDAPGAALRLLQAPLAPLRPHEVLIEVSACGVCR
                     TDLHLLDGELTQAVLPVTPGHEIVGRIVDAGAAVEGFRGGDRVGVPWLASACGSCSYC
                     EAGLENLCERAQFTGCTVDGGFARHAIADARFCLELPDRYDDAHAAPLLCAGLIGYRA
                     YRAAGGGANLGLYGFGAAAHLLGQVACAEGRRVFALTRPGDLDAQRFARELGAVWASG
                     SNEAPPEPLDAAIIFAPVGSLVPAALAAVRKGGRVVCAGIHMSDIPAFPYSLLWGERT
                     VCSVANLTREDGRAFMRVAAGIDLRPAIEPFALEQANDALGRLRRGELRGAAVLLPGA
                     AGAGP"
     gene            complement(1081..2364)
                     /locus_tag="PKN91_04305"
     CDS             complement(1081..2364)
                     /locus_tag="PKN91_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNS27327.1"
                     /translation="MKTSWKRETIAVHGGYSPDPTTRAVAVPIYQTVAYAFDDTQHGA
                     DLFDLKVEGNIYTRIMNPTQAVLEQRVAALEGGIGALALASGQAAVTYAIMTIAEAGD
                     NIVAASTLYGGTYNLFAHTLPQYGIEVRFADYREPNAFEPLIDARTKAIFCESVGNPL
                     GNVTDFEQLAKIAHAHGLPLIVDNTVPTPHLCRPFEHGADIIVHSLTKGMGGHGTSVG
                     GAIVDSGKFPWAEHKQRFRRLNEPDVSYHGVVYTEALGPAAYIGRARVVPLRNTGAAI
                     SPFNAFLLLQGIETLGLRIDRISENALAVATHLAGHEKVGWVRYAGLASHPDHALVKK
                     YLSGKAPGILTFGLRSGRAGGERFQDALELFTRLVNISDAKSLACHPASTTHRQLSPD
                     ELAKAGVSEDMVRLSIGIEHIDDLIADIDQALARA"
     gene            2633..3445
                     /locus_tag="PKN91_04310"
     CDS             2633..3445
                     /locus_tag="PKN91_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HNS27328.1"
                     /translation="MKPIRHAPVLVRALLGALLSCTGATAAGADAPWIEGRHYVLLDP
                     PVPTQVPPGKVEVVEVFSFGCPACNQFEPIMQQVKAGLPPEAEVVYVPASWNAAESWP
                     LFQRAFHVAQVLGIEAQVHGPMYAAIWETGELAVVDPLTHRLKVPRPTIGLVARFFAR
                     TAGVTQQAVLDAARSIEVDMKMMESDRLVQAYAVEGTPTLVVAGKYRVVNSSVRSVEE
                     LAALVRFLVERNRESVALAPSLGRSCATQNCAARASRAIIGGSATESPYAPE"
     gene            3432..4898
                     /gene="ccoN"
                     /locus_tag="PKN91_04315"
     CDS             3432..4898
                     /gene="ccoN"
                     /locus_tag="PKN91_04315"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HNS27329.1"
                     /translation="MPQNETYNDQVVRLFLLAAAVWGVIGMSIGVFVAAQLAWPALNF
                     ETSWLTFSRLRPDHTFGVIFAFGGSALMGTCYYVAQRTGHTRLALPRLANFTFWSWQL
                     VCILAMVTMPLGITQSKEYAEPEWLIDLLVAVVWVSFGVVFFATLARRRIKHIYVANW
                     YYGAFIIAVALLHIVNNIVLPISLTKSYPVYSGTVDAMVQWWYGHNAVAFFLTAGFLG
                     MMYYFVPRQAQQPLWSYRFSIVNFWALISVYMWAGSHHLMYTALPDWVQSVGMAFSIV
                     LLMPSWGSAANGLLTFNGAWRRVASDPAAKFMVIALVFYAAATTEGSLMAVKTVNSLS
                     HYTDWTVAHVHSGAIGWVAMITIGSLYAMAPRALGRPAMHSKRGMEWHFWLHVTGLVM
                     YVASMWTAGVTEGLMWRATDANGVLIYPFIDSLMAVRPFYLIRFFGGLLIVAGMVVMA
                     WNLWHTAAHARAHLIKPIPVPIPEPEPQQTPAPVPARA"
     gene            4909..5541
                     /gene="ccoO"
                     /locus_tag="PKN91_04320"
     CDS             4909..5541
                     /gene="ccoO"
                     /locus_tag="PKN91_04320"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HNS27330.1"
                     /translation="MAKGYKYFEAVEKHAGKLAILTAIMVSIGGLAEITPLFVEAHRI
                     TAAENVKPYDALRLAGKDIYVREGCYVCHTQMIRSLRAETQRYGAYSVSSESVYDRPH
                     QWGSKRTGPDLARLGGKYSDEWQRLHLLDPRSVVPQSNMPGYPWLADEPIDSDDIVAR
                     MRGLKRLGDPYTDDDIAAAPAALEGKTAMDALIAYLQGLGLPPQTAEEPT"
     gene            5538..5693
                     /locus_tag="PKN91_04325"
     CDS             5538..5693
                     /locus_tag="PKN91_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HNS27331.1"
                     /translation="MSPLWGQVAGIVTVMMMVIFISIWAWVWHAGHKQKYDALARLPM
                     SDSGEDS"
     gene            5690..6598
                     /gene="ccoP"
                     /locus_tag="PKN91_04330"
     CDS             5690..6598
                     /gene="ccoP"
                     /locus_tag="PKN91_04330"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HNS27332.1"
                     /translation="MSTFWSLWVMALVVFNLGVTLFLYYWAQKVEIPTLEDGTTGHVW
                     AHGALRESVRKLPGWWVVASAGLFACSFIYLLLYPGFGAWTGLLGWTQIDQHATQVAL
                     NEANVAELKGRFAQLSPEQLAANPLAQQIGGRLFIDNCAACHQRDARGNHLLGAPNLV
                     DGDWTWGGDDASITTSILEGRQGLMPPLGTLGADTVTDIAHYVLSLSGAPHDSARATT
                     GEAGFALCSACHGSDGKGNPIMGAPNLTDSIWTWSNGDLAAIERVITNGLHGVMPAWK
                     ARLNQDDTRVIIAWLRSQSGDRLARR"
     gene            6595..6801
                     /locus_tag="PKN91_04335"
     CDS             6595..6801
                     /locus_tag="PKN91_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27333.1"
                     /translation="MTHDGAIWYRQPILWLGGALFAAVVAGCIALVVLGARYDDEALS
                     TQGATVLKMPAGRADPPPDPEVPQ"
     gene            6798..9245
                     /locus_tag="PKN91_04340"
     CDS             6798..9245
                     /locus_tag="PKN91_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNS27334.1"
                     /translation="MNAAARGCWHCGEPLPDTDAPQARVAGQLHPVCCHGCRAAAEWI
                     DQLGLADYYRLRSAPAPRAASADDMPSNQAWSRPALERHVVRDLAGGRSEAVVLIEGL
                     RCAACVWLIERALAALPGLVTVQVNATARRARIVWERERGSLAAILDTLERTGYRPLP
                     LDAAALDDVRQREQRTALKQLVVAGFGAMQAMMYASGLYFGAFEGIDPAARELLRWLG
                     LLACTPVVFYSARPFFAGAVRTLRARRLGVDVPVAIAIALIYAASIYEAYRGTGEVYF
                     DSVSMFVLFLLFGRYLEMRARHRAGDLTDALARLTPTYADRIDASGKLERIGAIELVR
                     GDRVHVDTGSGIPADGVLESARCRVDESLVSGESAAVARQRGEPLVAGSMLLDGPIVM
                     RVERVGADTVLSGIVALVTRAATERPRLAAAGERAAAGFVARVLTLAALTATIWSLVD
                     PSRAFTATLAVLVVSCPCAFALAVPAAITRSLGVLARRGVLVVRPDAIENLARATHIV
                     FDKTGTLTEPTLRLESIEAHAGIDRDAALRLAAALARGSRHPVARAIAAGVAADIDLP
                     EVRAQRTEAGGGLEGIVDGRRLRLGRADFALAREAAPELADAVVLAVDEGPIAAFRLA
                     ERLRPDARAAIDALVAAGLTAEIASGDGDTKVAAVAHRLGIAQWRARQRPDEKLARLA
                     ELRAAGARVAAVGDGINDAPVLAGADVSIALAGGAELAQATSDIVLSGGRLGMLPQAR
                     VIARSTLRILRQNQRWAMIYNLSVVPLGALGFIPPWLAAIGMSMSSLVVVLNALRIGR
                     EPASRAVAAIAPVPAAT"
     gene            9242..9439
                     /gene="ccoS"
                     /locus_tag="PKN91_04345"
     CDS             9242..9439
                     /gene="ccoS"
                     /locus_tag="PKN91_04345"
                     /inference="COORDINATES: protein motif:HMM:NF015554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNS27335.1"
                     /translation="MNILVVMIPLSILLLVGAGIAFFWAVDHDQFDDFDTPRLLPLLD
                     DPDARVDASPGEAPPADPPAP"
     gene            9436..10176
                     /locus_tag="PKN91_04350"
     CDS             9436..10176
                     /locus_tag="PKN91_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNS27336.1"
                     /translation="MSDPITLGAAMLLGLIAGGHCILMCGGITAALGIGTAERADGRP
                     RLDLLAGYQLGRISSYALAGLVFGGALGVIVDWLDIEAVRHALRVLAGVALLAGALVA
                     FGSLRDPGTGLGRRLWPKLAPLGRRLLPVTNLPRAAAFGMIWGWMPCGILYTMLMIAG
                     LQLDAWRSPSTMVAFGLGTAPAMFATALGAQRFAGFAARPAARRVACLALLASAALTF
                     AGPWIAHYAPGIAHWLPFDCGVTGSSSP"
     gene            complement(10215..10808)
                     /locus_tag="PKN91_04355"
     CDS             complement(10215..10808)
                     /locus_tag="PKN91_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27337.1"
                     /translation="MTTIKAFARVLTCFTLAACVPTYTLVSPGRVAVSKGAMVVEPKS
                     AWNRVPNGPQTIPRQENWTHNGPLLESITFIGGLVPGEAIAKQKAKDDRQVPPFRADM
                     TPQDLVSMIESYYRIRVGAAVYETTDVRPVVFLGQPAVQVDFSYVVADEVKRLGRSKL
                     AIIDGKLFAMSLDGTALHYFADALPEFEEIVASAARR"
     gene            complement(10819..11991)
                     /locus_tag="PKN91_04360"
     CDS             complement(10819..11991)
                     /locus_tag="PKN91_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNS27338.1"
                     /translation="MAGWPASARVNPMSLAPLVHPGYVPADADERGLWQQCEEFEYEL
                     ASSNLLLQDPTVQSYVHAITNRLLGERDFGIRTYVVRDAEFNASMFPNGMMLVHTGLL
                     ARMRNEAQLAAVLGHECGHYLRRHSLERHRDLKRKSAAAAIVGLAAAGATGATGSNWF
                     DLANSINTMLVLSVYTFSRGQESEADAYGLKLLAECGYMPGAAAAVWAQIIAERKASA
                     VARKKRYRDHSASEYSTHPPSQERMEDLAASADDIASRVHDSREFEDRRAEWLALAAP
                     IRFSLLEEQIKLNDPGASLYLLESLAVDGWTGTLRFFEGEAYRLRGGVDDVARATAAY
                     LAAVAFTDAPPEAWRAHGYAQIQSGHADDGRRALAHYLELKPDAVDAAMVRFTLVQ"
     gene            complement(12064..12804)
                     /locus_tag="PKN91_04365"
     CDS             complement(12064..12804)
                     /locus_tag="PKN91_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27339.1"
                     /translation="MNTRRILVVAAAVMSVTACTTTRQVADTSFTPPQGDYRLIVMQP
                     DISVGLLTAGGVVEQREDWTDQATANVLQSLRAMHAARGGNAMIAATREESGWDVAEV
                     TDLVRLHEAVGASITLHKYLGLALPTKKNRFDWTLGEQAVEFGRATRFDYALFLHAQD
                     SFSSAGRVALQVAGFLSCAVGVCVMATGGQQSAFASLVDLKTGQVVWFNTLASSVGDI
                     RTAEGADKLVKALLGDMKPGAPVGKPAG"
     gene            12935..14083
                     /gene="cfa"
                     /locus_tag="PKN91_04370"
     CDS             12935..14083
                     /gene="cfa"
                     /locus_tag="PKN91_04370"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF008686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HNS27340.1"
                     /translation="MATTMPVPAAKAPTAEYRALAQLLEPADVALGGTRPWDITVHDP
                     RMPARVLAQGALGAGESYMDGWWDCERLDEMLTRVLCLGVDRRLRSMRVLATALLARL
                     RNPQSPRRAYIVGRSHYDIGDDLYERMLDARMIYSCAWWLRAANLDEAQEHKLDLVCR
                     KLGLEPGMQVLDIGCGWGGAALFAAERYGVTVTGITVSEHQAAAARLRCSDLPVEIRL
                     DDYRRLTGRYDRIWSLGMFEHVGARNYRTYFDKVRELLAPDGLFLLHTIGSNRSVTVT
                     DPWIEKYIFPNSMLPSIAQIGQAAEPRWVLEDWHGFGPDYDRTLLEWHRRFEAGWSQI
                     AAKYGERFRRMWRFWLLSSAASFRARRAQLWQFVYSPSGRPGGYQAVR"
     gene            14083..>15435
                     /locus_tag="PKN91_04375"
     CDS             14083..>15435
                     /locus_tag="PKN91_04375"
                     /inference="COORDINATES: protein motif:HMM:NF036723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNS27341.1"
                     /translation="MAVAAPFVQTAPATPRAWSDDWLLRSVLRHRLPPEVLLAISPAL
                     LEMEALAAGPLAQLQMADREREPVHTPWDAWGNRIDRIEATPLWQAAEPLAARHGLVA
                     IPYEKQHGRCSRMHQFALVHLFHPVSDMYTCPLAMTDGAARALLESDNAALIERALPH
                     LTSRDPASFWTSGQWMTESTGGSDVGASQSRAVRTSDGSWRLAGKKWFTSAATSQMAL
                     TLARPEGNGPGGSGLAMFYLETRDTDGRLNGIRIERLKDKLGTRKVPTAELTLDDAHA
                     QLVAGTSHGTRTIEPMLRITRAWNSVCAASLMRHGYLLARDYAAKRRAFGVRLIELPL
                     HAETLADLDAESAGALLLTFELIDLLGRQESHELDDAGAALLRLLTPVTKAVTGRQAV
                     AAASETLEAFGGAGYLEDTLLPALLRDAQVVPSQIGRTCASRMADPALRAATETAVRA
                     V"
     assembly_gap    15436..15535
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15536..15800
                     /locus_tag="PKN91_04380"
     CDS             <15536..15800
                     /locus_tag="PKN91_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27342.1"
                     /translation="RMADPALRAATETAVRAVDRGIAWQRTAADDERALQAGARRLVL
                     SLGRAFELALLAELADAAAIEGDDRSAARRACLRLQAAGYFFV"
     gene            complement(16094..16951)
                     /locus_tag="PKN91_04385"
     CDS             complement(16094..16951)
                     /locus_tag="PKN91_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNS27343.1"
                     /translation="MATSEQTDAAPGRPRRAYADGPFGQAHFQVLGEGRPLILLHQAP
                     MASGQFDNVMVPLVRRGFRAIAIDMPGFGMSDPTPDTPTVGDYARVVPAVLDHLGLER
                     AAVLGHHTGALAATEAAILYPQRVSALIINGPLLVTEEERDKFRQNLHQWELGYVARP
                     HAAHMVELFDIRDRMAGGSIPPARLSDYVVQALIGRGAFWYGHHAAYMYDQAARLPKI
                     QQPTLILTNTGDLIYDHALRAQALRPDFAFAALEGGGVDIVDQQPEAWADAVAAFLDN
                     APDETGRTC"
     gene            complement(17027..17308)
                     /locus_tag="PKN91_04390"
     CDS             complement(17027..17308)
                     /locus_tag="PKN91_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27344.1"
                     /translation="MERVFPEQAQVQDLQSLVHRSVQLQQLADDCRQHVSVEGFSLNF
                     RVIVVEVCVQDCSPAARAASLFDMGYKYADVKPPAEVLAALDEFECEVG"
     gene            17586..18314
                     /locus_tag="PKN91_04395"
     CDS             17586..18314
                     /locus_tag="PKN91_04395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNS27345.1"
                     /translation="MTNIAEPVFLVTGSASGIGKAIADRLTVAGHRVRRLDKSFDATS
                     GAGIALDITDEAAIAHAVAEIGVDGPIGALVNCAGIGGLGTVTDISMEHWDRVMAVNL
                     RGTVLMCRAVLPGMIGRGRGSIVNVGSTFGLVARGGCVAYGVSKAAVIHLTRSMAVDL
                     ADSGVRVNCVCPGLIETPMTESLFRPGEEERRRANIEAHALRRPGKPDEVATAVEYLL
                     SDAASFVTGAVLPVDGGYTAGKWV"
     gene            complement(18348..19448)
                     /locus_tag="PKN91_04400"
     CDS             complement(18348..19448)
                     /locus_tag="PKN91_04400"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HNS27346.1"
                     /translation="MTVPGKAGAWLAVLPGAALALALALTAKIGTQEIGAALANGRPS
                     PVSPVLVGIVIGVLWRHFIGVGARTEAGVRWILGTLLLVGIALVGLRLTLPGLAGVGR
                     VAIPVVVVCILVALAVSQLVGRAFGLSAELRRLLAVGSAVCGCTAIVATAPVIRARAI
                     DTGTALTCVVLIGSTGMLLYPWIAALVFGTDPLPAGVFLGSAIHDTSQVIGASLIYSQ
                     QFASPDAVAVASATKLLRNLSIIVLVPLFGWLAHAETTRQPDTASSARRRPLVPWFIV
                     AFVALVVVRALGDQFVADRAALAGGWGATLDVAQTLSELFMICGMTAVGLNLSLANLG
                     QVGMRPFAAALVIAVATAACSLGLTALILAVA"
     gene            complement(19449..19796)
                     /locus_tag="PKN91_04405"
     CDS             complement(19449..19796)
                     /locus_tag="PKN91_04405"
                     /inference="COORDINATES: protein motif:HMM:NF040386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6356 family protein"
                     /protein_id="HNS27347.1"
                     /translation="MRNSIVIRPSAVQYFAILLRTRTSKVSAVNIHKAFTEHPRSVGE
                     TYLQHMGVAGRCGLSMMTAGFACLIHAALPFLFTNTASDCLTRLHERMVTKRSRLRQL
                     SSTPDSLGASSPR"
     gene            19824..20291
                     /locus_tag="PKN91_04410"
     CDS             19824..20291
                     /locus_tag="PKN91_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNS27348.1"
                     /translation="MDRIDKAILDLLQRDASLGVADLAEKVGLSKAPCWRRIRRLQER
                     GVIRGTVALLDPKLLNVGTTVFVTIKTANHSEAWYERFLQTLDSIPEVVEIYRMSGDV
                     DYLLRVVVPDIEGYGAVYAQLIAGCEFLDISASFAMGSIRHTTALPLNHLAPG"
     gene            complement(20262..20609)
                     /gene="mnhG"
                     /locus_tag="PKN91_04415"
     CDS             complement(20262..20609)
                     /gene="mnhG"
                     /locus_tag="PKN91_04415"
                     /inference="COORDINATES: protein motif:HMM:NF015302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HNS27349.1"
                     /translation="MSGVELPMWAAVPAALLLIASGLLTAIGSLGLLRLPSFQARMHG
                     PSMGATLGCGCMLFASMFVSSAIAGRPVLHEFLITVFVVMTAPATAMLLMRASIYRAR
                     NKAGSAGGEVVQR"
     gene            complement(20606..20884)
                     /locus_tag="PKN91_04420"
     CDS             complement(20606..20884)
                     /locus_tag="PKN91_04420"
                     /inference="COORDINATES: protein motif:HMM:NF004812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HNS27350.1"
                     /translation="MTLLDYAAWFASGCFGLAMLFATWRVIRGPAAQDRVLGLDTLYV
                     NGMLMLLALGIRSGSGFYFEIALLIALFGFVGSVALAKFLLRGEVIEP"
     gene            complement(20881..21369)
                     /locus_tag="PKN91_04425"
     CDS             complement(20881..21369)
                     /locus_tag="PKN91_04425"
                     /inference="COORDINATES: protein motif:HMM:NF006520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HNS27351.1"
                     /translation="MKSFAPVLAIALAVMWLLLNDTVSPGHVVLGIGLAIALTLWSAK
                     LRPLRPHLYRPLLAIGLLWTVFVDIVRSNIAVARIILGLVGERRIRSAFLDIPLELRD
                     PHGLAVLATIVTSTPGTVWVDFNAERGQLTLHVLDLRDEEVWIRIIKDHYERPLLGIF
                     QP"
     gene            complement(21366..23042)
                     /locus_tag="PKN91_04430"
     CDS             complement(21366..23042)
                     /locus_tag="PKN91_04430"
                     /inference="COORDINATES: protein motif:HMM:NF009309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HNS27352.1"
                     /translation="MTDLLQHLPMVPIVAPLVAAATILFLTERSRRIRIAIAGLSMFV
                     QLCAAIALLTLTTSAFPDVWTRGVGVYAIGAWQPPFGIVLVIDRLTGMMLTLTAVVGL
                     AAFVNSLAYWDRVGPHFHTLFQLLLMGVNGAFLTGDLFNLFVFFEILLAASYGLLLHG
                     SSTQRVKVSLHYIIVNLVGSLLFLIGVSLVYGVTGSLNIAELPAATSQLAGKERALFD
                     AATSILAVAFLIKAGAWPLNFWLPGTYAAAAAPVAAVFGILTKVGVYVLLRVGSLGAG
                     ADVLAAIEGPAMLRPELFRGGMYYLGILTMGYGIFGMLAAQHLARLVAFSAIVSSGLL
                     LTVIGLDIPALMAPALFYLIGSVLATAAFFMLAGMTERAWRSPAQGSSPQAATPEPEV
                     PPHYVPFGVRRTPALHSQDDEVGVAIPAAMAFMGIMFAFCVLLVTGSPPLSGFVAKFA
                     LLSAAFHAADDAALAGPLWVLAGVTLATGLASLVALTRIGMRLFWSVTGRNTPRLRVL
                     EAGPVAVLVVLCIMLTVAAGPVMAFLDEAAASLRAPQIYIETVLAGKEVL"
     gene            complement(23039..>23292)
                     /locus_tag="PKN91_04435"
     CDS             complement(23039..>23292)
                     /locus_tag="PKN91_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003250128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HNS27353.1"
                     /translation="IGLALLSYAVNLFIFGMGRLTIGRAPILAGADATDVALYADPVP
                     QALVLTAIVIGFATTALLLVVLLAARGLTGTDHVDGREP"
BASE COUNT         3858 a         7964 c         7738 g         3632 t
ORIGIN      
        1 aactacctca cctcactcca ctcgtcagta ggttctgccg cccagactcc tagggcccgg
       61 cgccggcggc gcccggcagc agcaccgcgg cgcctcgcag ctcaccgcgc cgcaagcgac
      121 cgagcgcgtc gttcgcctgc tcgagggcaa acggttcgat cgccggccgc agatcgatcc
      181 ccgcggcaac gcgcatgaat gcgcgaccat cttcgcgcgt gaggttcgcc accgagcaga
      241 ccgtgcgctc gccccacagc agcgaatagg gaaaggccgg aatgtcgctc atgtggatgc
      301 cggcgcagac gacccgcccg cccttgcgca cggccgcgag cgctgccggc acgagtgagc
      361 cgaccggcgc gaagatgatg gcagcatcga gcggctcggg cggcgcttcg ttcgagccgc
      421 tggcccagac tgcgccgagc tcgcgggcga agcgctgcgc atcgaggtcg ccgggccgcg
      481 tcagcgcaaa gacgcgccgg ccttctgcgc acgccacctg gccgagcagg tgcgcggccg
      541 caccgaatcc gtacagcccg aggttcgcgc cgccgccagc cgcgcgatac gcgcgatagc
      601 caatgaggcc ggcgcacagc agcggcgcgg catgtgcatc gtcatagcgg tcgggcagct
      661 cgaggcagaa gcgcgcatcg gcgatcgcgt gccgggcgaa gccgccatcg acggtgcagc
      721 cagtgaactg tgcgcgctcg cacaggtttt ccagcccggc ctcgcagtag ctgcaactgc
      781 cgcaggccga cgccagccac ggcacaccga cgcgatcgcc gccgcgaaac ccctcgaccg
      841 ccgcgcctgc atcgacgatg cgcccgacga tctcgtgccc cggcgtcact ggcaggacgg
      901 cctgagtcaa ctcgccgtcg agcagatgca gatcggtgcg gcacacgccg caggcgctca
      961 cttcgatcag cacctcatgg ggccgcagcg gcgcgagcgg cgcctgtagc agccgcaacg
     1021 cggcacccgg tgcatcgagt cgcatcgccc gggtggccgt ttgcctcatg tcctgctcga
     1081 ttacgcgcgg gcgagcgcct ggtcgatatc ggcgatcagg tcatcgatgt gctcgatgcc
     1141 gatcgagagc ctgaccatgt cctcgctgac acccgccttc gcgagttcgt ccggcgagag
     1201 ctgacgatgg gtggtcgagg ccggatggca cgcgagcgat ttcgcgtcac tgatattgac
     1261 gaggcgtgtg aagagctcga gcgcgtcctg gaatcgctcg ccacccgccc ggccactgcg
     1321 caggccgaaa gtcaggatgc cgggggcctt gccggagagg tatttcttca cgagagcgtg
     1381 gtccggatgc gaggcgaggc ccgcgtagcg gacccaaccc actttctcgt ggcccgcgag
     1441 atgcgttgcc accgccagcg cattctcgct gatgcgatcg atgcgcagcc cgagtgtctc
     1501 gataccctgc agcagcagga acgcgttgaa cggggagatc gccgcgcccg tattgcgcag
     1561 cggcacgacg cgcgcgcgac cgatgtacgc ggccggcccc agcgcctcgg tgtagacgac
     1621 gccgtgatag ctcacatcgg gctcgttcag gcgccggaag cgctgcttgt gctcggccca
     1681 cggaaacttc ccggaatcga cgatcgcgcc gccgacgctc gtgccgtggc cgcccatgcc
     1741 cttggtcagc gagtgcacga tgatgtcggc gccgtgctcg aacggccggc acaggtgcgg
     1801 cgtcggcacc gtgttgtcga cgatcagcgg cagtccatgg gcatgcgcga tcttcgcgag
     1861 ctgctcgaaa tcggtcacgt tgccgagcgg attgccgacc gactcgcaga agatcgcctt
     1921 ggtgcgcgca tcgatcagtg gttcgaacgc gttcggctcg cggtagtcgg cgaagcgcac
     1981 ctcgatgccg tattgcggca gcgtgtgcgc gaacaggttg taggtgccgc catacagcgt
     2041 gctcgccgcg acgatattgt cgccggcctc ggcgatcgtc atgatcgcat aggtgaccgc
     2101 ggcctgcccg gaggcgagcg cgagtgcgcc gatgccccct tccagcgcgg cgacgcgctg
     2161 ctcgagcacc gcctgcgtcg gattcatgat ccgcgtatag atgttgcctt cgaccttgag
     2221 gtcgaacagg tcggcaccgt gctgcgtgtc gtcgaacgca tacgccacgg tctggtagat
     2281 cggcacggca acggcgcgcg tcgtcgggtc gggcgagtaa ccgccgtgta ccgcaatcgt
     2341 ctctctcttc cagctggttt tcatggtcgc tgatcagtct ccgcggtcgg aacgccggtc
     2401 catcgagcgt acccgatgac agggaatggt gcgcataagt gaaatccctg cccgcggcac
     2461 ggggctcccg gatcgccgcc tgcacgatga cgatccggcc gcccgggggc acgatgcgtg
     2521 cccggctcgt gcaagtcgcg tcgtcaaagg catcgagtac gttgtcgatc gctcggcgga
     2581 cacgcacgcg caggtgccgc tagaatattg gcgctcaaag aacgaggtgc ccgtgaaacc
     2641 gatccgccat gcccccgtac tggtgcgcgc actgctcggt gcgttgctgt cctgcaccgg
     2701 cgcaactgcc gccggtgccg acgcgccctg gatcgaaggc cggcactacg tgctgctcga
     2761 cccgccggtg cccacgcagg ttccgcccgg caaggtcgag gtcgtcgagg tgttctcgtt
     2821 cggctgcccg gcctgcaacc agttcgagcc gatcatgcag caggtcaagg cagggctgcc
     2881 gcccgaggcc gaggtcgtgt acgtgccagc ctcgtggaat gccgcggagt cctggccgct
     2941 cttccagcgc gcattccacg tcgcgcaggt cctcggcatc gaggcgcagg tccacggacc
     3001 catgtacgcg gccatctggg aaaccggcga actcgcggtg gtcgatccgc tgacccaccg
     3061 gctcaaggtg ccgcggccga ccatcgggct cgtcgcccgc ttcttcgcgc gcacggccgg
     3121 cgtgacgcag caggccgtac tcgacgccgc caggtccatc gaggtcgaca tgaaaatgat
     3181 ggagtcggac cggctcgtcc aagcctacgc ggtcgagggc acgccgacac tggtcgtcgc
     3241 gggcaagtac cgggtcgtca attcgagcgt acgcagcgtg gaggaactgg ccgcgctggt
     3301 gcgcttcctc gtcgaacgca acagagagtc agttgcactg gccccgagcc tgggccgcag
     3361 ctgtgctacc cagaactgcg cggcgcgtgc gtcgcgcgcc ataataggcg gttcagccac
     3421 ggagtccccg tatgccccag aatgagactt acaatgacca ggtcgtacgc ctgttcctgc
     3481 tcgccgcggc cgtctggggt gtcatcggca tgtcgatcgg cgtgttcgtc gccgcacaac
     3541 tcgcctggcc cgcactcaat ttcgaaacgt cctggctcac cttcagccgc ctgcgacccg
     3601 atcacacctt cggcgtgatc ttcgccttcg gcggctcggc gctgatgggt acctgctact
     3661 acgtcgcaca gcgcaccggg cacacgcgcc tcgccctgcc acgcctcgcc aacttcactt
     3721 tctggagctg gcagcttgtc tgcatcctgg cgatggtcac gatgccgctc ggcatcaccc
     3781 agagcaagga atatgccgag cccgaatggc tgatcgacct gctggtggcg gtcgtctggg
     3841 tcagcttcgg cgtggtgttc ttcgccacgc tcgcgcgccg gcgcatcaag cacatctacg
     3901 tggccaactg gtactacggc gcgttcatca ttgccgtcgc cctgctgcac atcgtcaaca
     3961 acatcgtgct gccgatctcg ctgaccaagt cctatccggt gtactcgggc accgtcgatg
     4021 caatggtgca gtggtggtac gggcacaatg ccgtcgcatt tttcctgacc gccggcttcc
     4081 tcgggatgat gtactacttc gttccacgcc aggcgcagca gcccctgtgg agctaccgct
     4141 tctcgatcgt caatttctgg gcgctgatct cggtctacat gtgggccgga tcgcaccacc
     4201 tgatgtacac ggccctgccc gactgggtgc agtcggtcgg catggcgttc tcgatcgtgc
     4261 tgctgatgcc gagctggggc tcggcggcga acggtctgct gaccttcaac ggcgcctggc
     4321 ggcgggtcgc atctgatccc gccgcgaaat tcatggtgat cgcgctggtg ttctacgcgg
     4381 ccgcaaccac cgagggctcg ctgatggccg tcaagaccgt caattccctc tcccactaca
     4441 ccgactggac cgtggcgcac gtgcactccg gcgcgatcgg ctgggtggcg atgatcacga
     4501 tcggttcgct ctacgcgatg gcgccgcgcg cgctcggccg accggccatg cattcgaagc
     4561 gtggcatgga gtggcacttc tggctgcatg tcaccggact cgtgatgtac gtcgcctcga
     4621 tgtggaccgc gggggtgacc gagggcctga tgtggcgcgc aaccgatgcg aacggggtcc
     4681 tgatctatcc gttcatcgac agcctgatgg ccgtgcgacc gttctacctg atccgcttct
     4741 tcggcggatt gttgatcgtg gccggcatgg tagtcatggc ctggaacctg tggcacaccg
     4801 ccgcccacgc ccgggcccac ctcatcaagc cgataccggt gccgattccg gagccggaac
     4861 cgcagcagac gccggcgccc gtgccggccc gggcctgagg agcgcatcat ggccaaaggc
     4921 tacaagtact tcgaagccgt cgagaagcac gccggcaagc tcgccatcct caccgctatc
     4981 atggtgtcga tcggcggcct cgccgagatc accccgctgt tcgtcgaggc gcaccgcatc
     5041 accgcggccg aaaacgtgaa accctacgat gcgctgcgtc ttgccggcaa ggacatctac
     5101 gtgcgcgagg gctgctatgt ctgccacacg cagatgatcc gttcgctgcg agccgagacc
     5161 cagcgctacg gggcgtactc ggtgtcgagc gaatcggtct acgaccgtcc gcaccagtgg
     5221 ggatccaagc gcaccgggcc cgatctcgcg cggctcggcg gcaagtactc cgacgagtgg
     5281 cagcgcctgc acctgctgga cccgcgctcg gtcgtgcccc agtccaacat gccgggctat
     5341 ccgtggctcg ccgacgagcc gatcgacagc gacgacatcg tggcgcgcat gcgcggactg
     5401 aagcgcctcg gcgacccgta cacggacgac gacattgcgg cggcaccggc ggcgctcgag
     5461 ggcaagaccg cgatggatgc actgatcgcc tacctgcagg gtctcggctt gccgccgcag
     5521 acagcggaag agccgacatg agtcctctct ggggacaggt ggccggcatc gtgaccgtga
     5581 tgatgatggt gatcttcatc agcatatggg cctgggtgtg gcacgcgggt cacaagcaga
     5641 agtacgacgc gctcgcgcgc ctgccgatgt cggattccgg ggaggactca tgagcacgtt
     5701 ctggtcgctg tgggtgatgg ccctggtggt attcaacctc ggcgtcacgc tgttcctgta
     5761 ctactgggcg cagaaggtcg aaatcccgac gctggaagac ggcacgacgg gccacgtctg
     5821 ggcgcatggc gcgctgcgcg agagcgtacg caagctgccg ggctggtggg tcgtcgcgtc
     5881 ggcgggcctg ttcgcctgct cgttcatcta cctgctgctg tacccgggat tcggcgcgtg
     5941 gacgggtctg ctcggctgga cccagatcga ccagcatgcg acgcaagtcg cgctgaacga
     6001 ggccaacgtg gccgaactga agggccgctt cgcgcagttg tcgccggaac agcttgcggc
     6061 gaacccgctc gcgcagcaga tcggcgggcg actgttcatc gacaactgcg ccgcctgcca
     6121 tcaacgcgat gcacgcggca accatctgct aggcgcaccg aacctcgtcg acggcgactg
     6181 gacatggggc ggcgacgacg cctcgatcac caccagcatc ctcgaagggc gccagggcct
     6241 gatgccgcca ctcggcacgc tgggcgcgga cacggtcacc gatatcgcgc actacgtgct
     6301 cagcctgtcg ggcgcgccgc acgattccgc cagggccacg accggcgaag ccgggtttgc
     6361 gttgtgctcc gcctgccacg gcagtgacgg caagggcaac ccgatcatgg gcgcaccgaa
     6421 cctcaccgat tcgatctgga cctggagcaa cggcgatctc gcggctatcg agcgcgtcat
     6481 caccaatggc ctgcatggcg tgatgccggc gtggaaggcg cgactcaacc aggacgacac
     6541 gcgcgtgatc atcgcctggc tgcgatcgca gtcgggcgat cgactggcac ggcggtgacc
     6601 cacgacggcg caatctggta ccgccagccg atcctgtggc tcggcggcgc gctgttcgcc
     6661 gcggtcgtcg caggctgcat cgcgttggtc gtgctcggcg cccgctatga tgacgaggcg
     6721 ctgtccacgc aaggggccac ggtcctgaag atgccggccg ggcgcgccga cccaccgccc
     6781 gaccccgagg ttccgcaatg aacgccgcgg cgcgcgggtg ctggcactgc ggcgaaccgc
     6841 tgccagacac cgatgcgcca caggcacgcg ttgcgggaca gcttcatccg gtgtgctgcc
     6901 acggctgccg tgccgcggcg gagtggatcg accagctggg actcgccgac tattaccgcc
     6961 tgcgcagcgc gcctgcgccg cgcgcggcat ccgctgacga tatgccttcg aaccaggcgt
     7021 ggtcgcgacc cgcgctcgag cggcacgtcg tgcgtgacct cgccggcggc cgcagcgaag
     7081 cggtggtgct gatcgagggc ctgcgctgcg cggcctgcgt gtggttgatc gagcgcgccc
     7141 tcgccgcgct gcctggcctc gtcaccgtgc aggtgaacgc caccgcccgg cgcgcgcgca
     7201 tcgtgtggga acgggaacgg ggctcgctgg ccgcgatact cgacaccctg gagcgcacgg
     7261 gctaccggcc actgccgctc gatgcggcgg cgctcgacga tgtgcgccag cgcgagcagc
     7321 gtaccgcgct caagcagctg gtcgtggccg gattcggcgc catgcaggcg atgatgtacg
     7381 cgagcggcct gtacttcggc gccttcgaag gcatcgaccc ggccgcgcgc gaactgctgc
     7441 gctggctcgg cctgctcgcc tgcacgccgg tggtgttcta ttcggcccgc cctttctttg
     7501 cgggcgccgt gcgcacgctg cgcgcccggc gcctcggcgt ggacgtgccc gtcgcgatcg
     7561 ccatcgcgct gatctatgcc gcgagcatct acgaggccta ccgtggcacc ggcgaggtgt
     7621 acttcgactc ggtcagcatg ttcgtgctct tcctgctgtt cggccgctac ctcgagatgc
     7681 gcgcgcgcca ccgcgccggc gacctgaccg acgcgctcgc ccggctgact ccgacctatg
     7741 ccgaccgcat cgacgccagc ggcaagctcg agcgcatcgg cgcgatcgaa ctcgtgcgcg
     7801 gcgaccgcgt gcatgtcgat acgggcagcg gcatcccggc cgacggcgtg ctcgaatccg
     7861 cccgctgccg cgtcgacgaa tcgctcgtct cgggtgagtc cgcggccgtt gccagacagc
     7921 gcggcgagcc gctcgtcgcc ggcagcatgc tgctcgacgg accgatcgtg atgcgcgtcg
     7981 agcgcgtcgg cgccgacacg gtgctctccg gcatcgtcgc gctggtcacc cgggccgcca
     8041 ccgagcggcc gcgcctcgcc gcggcgggcg agcgcgccgc cgccggtttc gtcgcgcgcg
     8101 tactgaccct tgcggcgctc accgcgacga tctggagctt ggtcgatccg tcgcgcgcat
     8161 tcaccgccac actcgccgtg ctggtggttt cctgcccgtg cgccttcgcg ctcgcggtac
     8221 ccgcggcgat cacgcgctcg ctcggtgtac tcgcgcggcg cggcgtgctc gtcgtgcgcc
     8281 cggatgcgat cgaaaacctg gcgcgcgcga cccacatcgt gttcgacaag accggcacgc
     8341 tcaccgaacc gaccctgcgg ctcgagagca tcgaggcgca cgcgggcatc gatcgcgacg
     8401 ccgcgctgcg gctcgcggca gcgctggcgc gcggcagtcg ccacccggtg gcgcgggcga
     8461 tcgcggccgg cgtcgccgcc gacatcgacc tgcccgaggt gcgggcgcaa cgtaccgagg
     8521 cgggtggcgg cctcgaaggc atcgtcgacg ggcgtcgcct gcggctcggc cgtgccgatt
     8581 tcgcgctggc gcgcgaggcc gcgccggaac tcgcggacgc cgtcgtgctt gcggtcgacg
     8641 agggaccgat cgcggcgttc cggcttgccg agcgcctgcg ccccgatgcg cgcgcggcga
     8701 tcgatgcgct ggtggccgct gggctcactg ccgagatcgc gagcggcgac ggcgacacaa
     8761 aggtcgcggc cgtcgcccac cgcctcggca tcgcgcaatg gcgcgcgcgc cagcgtcccg
     8821 acgaaaagct ggcacgcctc gccgagttgc gtgccgcagg agcgcgcgtc gccgcggtgg
     8881 gcgatggcat caacgacgcg ccggtgctgg ccggtgccga cgtgtcgatc gcgctcgccg
     8941 ggggcgccga actcgcgcag gccacgagcg acatcgtgct gtccggcgga cgcctcggga
     9001 tgctgccgca ggcgcgcgtc attgcccgca gtacgctgcg catcctcagg cagaaccagc
     9061 gctgggcgat gatctacaac ctctcggtcg tgccgctcgg cgcgctcgga ttcattccgc
     9121 cgtggctcgc ggcgatcgga atgtcgatga gttcgctcgt cgtcgtgctg aatgcgctgc
     9181 gcatcgggcg cgagccggcg tcgcgtgcgg tcgcggccat cgcgccggtg ccggcagcca
     9241 catgaacata ctcgtcgtca tgatcccgct gtcgatactg ctgctggtcg gtgccggcat
     9301 cgccttcttc tgggccgtcg accacgacca gttcgacgat ttcgacacac cgcgcctgct
     9361 gcctctgctc gacgaccccg atgcgcgggt cgacgcgtcg cccggggagg cgccgcccgc
     9421 ggacccgccc gcgccatgag cgacccgatc acgctcggcg cggcaatgct gctcggcctc
     9481 atcgccggcg gtcattgcat cctgatgtgc ggcggcatca cggccgcgct cggcatcggc
     9541 accgctgagc gcgcggacgg tcggccgcgt ctcgacctgc tcgccggcta ccagctcggc
     9601 cgtatctcct cctatgcgct cgctggcctg gtatttggcg gcgcgctggg cgtgatcgtc
     9661 gactggctcg acatcgaagc ggtgcggcat gcgctgcgcg tcctcgcggg cgtagcgctg
     9721 ctcgccggcg cactggtcgc attcggcagc ctgcgcgatc cgggcaccgg acttggccgg
     9781 cgcctgtggc cgaagctcgc gccgctcggc cgccggctgc tgccggtcac gaacctgccg
     9841 cgcgcggccg ctttcggcat gatctggggc tggatgccct gcggcatcct ctacacgatg
     9901 ttgatgatcg cgggtctgca actcgacgcg tggcgctcgc cgtcgacgat ggtcgccttc
     9961 ggcctcggca ccgcaccggc gatgttcgcc accgcgctcg gcgcgcagcg cttcgccggc
    10021 ttcgcggcgc ggcccgcggc ccggcgcgtg gcgtgcttag cgctgctcgc ctcagccgcg
    10081 ctcaccttcg cgggcccgtg gattgcgcat tatgcgcccg ggatcgcgca ctggctgccg
    10141 ttcgactgcg gcgtcacagg cagcagctca ccctgatcgg ccagtcgggg cccgccgggg
    10201 ccgtgcgcgg gcctctagcg ccttgccgcg gaagccacta tctcctcaaa ctcgggcaat
    10261 gcgtccgcga agtaatgcag tgccgtacca tcgagcgaca tcgcaaagag cttgccgtcg
    10321 atgattgcaa gtttcgagcg gccaagccgc ttcacctcat ctgcaacaac gtaggaaaag
    10381 tcgacctgca cggcgggttg cccgagaaag acgactggcc gcacatccgt cgtctcgtac
    10441 actgccgctc cgaccctaat tcggtagtac gactcgatca tcgagaccag gtcctggggt
    10501 gtcatgtctg cgcggaacgg aggcacctga cggtcgtcct tcgccttctg cttggcaatc
    10561 gcctcaccgg gcaccagtcc gccaatgaat gtaatcgact cgagcaatgg tccattgtgt
    10621 gtccagttct cctggcgcgg aatggtctgt ggcccgttcg gcaccctgtt ccaggccgac
    10681 ttcggctcaa cgaccattgc tcccttcgac acagccacgc ggcccggcga caccagcgtg
    10741 taggtaggca cacaggcggc tagtgtgaag cacgtaagga cccgcgcgaa agccttgatg
    10801 gtagtcattc tcatcccttc attgcaccag cgtgaagcgc accatcgccg catctacggc
    10861 atccggcttc agttccagat agtgggcaag tgcgcgccgg ccgtcgtcgg catggcccga
    10921 ctggatttgc gcatagccat gcgcacgcca cgcctcgggc ggcgcgtcag taaaggctac
    10981 ggccgcgaga tacgccgccg tcgcgcgcgc cacgtcatca acaccgccgc gcagacgata
    11041 cgcctcgccc tcgaagaatc tgagagtgcc ggtccaaccg tccaccgcga ggctttcaag
    11101 cagatagagg ctcgcgccgg gatcgttgag cttaatctgc tcctcgagta gcgagaagcg
    11161 gatcggagcc gctagcgcca gccattcagc gcggcggtcc tcgaactccc gcgagtcatg
    11221 cacgcgcgag gcgatgtcgt cagccgaggc cgcaaggtcc tccatccgct cctgcgatgg
    11281 cggatgtgtc gaatactccg aggctgaatg gtcccggtag cgcttcttcc gcgctaccgc
    11341 actcgccttg cgctcggcga tgatctgcgc ccagaccgcc gcggcggcac cgggcatata
    11401 gccacactcc gccagcagct tgagaccgta ggcatcggct tcgctctcct ggccacgact
    11461 gaacgtatag acggagagga ccaacatcgt gttgatactg tttgcgaggt cgaaccagtt
    11521 gctgccagtc gcccctgtgg cgccggctgc tgcgagcccg acaatggcgg ctgcagcgct
    11581 cttacgcttc aggtcgcggt gtcgctccaa cgaatgccgc cgcaggtagt gaccgcattc
    11641 gtgcccaagc accgcggcga gctgcgcctc gttgcgcatg cgtgcgagaa gtccggtgtg
    11701 cacaagcatc atgccattcg gaaacatcga ggcattaaac tcggcgtcgc gcacgacata
    11761 cgtccggatg ccgaagtccc gctcaccgag tagacgattg gtgatggcat gcacatagga
    11821 ctgcaccgtc gggtcctgca gcagcagatt cgacgacgcc agctcatact cgaactcctc
    11881 gcactgctgc cagagaccac gttcatccgc gtcggcgggc acatatcctg gatgcacgag
    11941 cggcgcgagg ctcatcgggt tgacgcgcgc ggatgccggc cagccggcca cgaccaaccc
    12001 acaaccgcag agcccttgtg tcaggacgtc ccgccgcctc aggaaaccgg ccacgctttc
    12061 cgctcagccg gctggtttgc caacaggtgc gccgggcttc atgtcgccca acagcgcctt
    12121 caccaacttg tccgcgcctt cggcagtgcg aatatcgccg accgacgagg cgagtgtgtt
    12181 gaaccacacg acctgcccgg tcttgaggtc gaccaaggat gcgaacgcgg actgttggcc
    12241 gccggtcgcc ataacgcata cgccgaccgc gcaactcagg aatcccgcga cctgcagcgc
    12301 aactctaccg gccgacgaaa aactgtcctg tgcatgcagg aataaggcgt agtcgaaccg
    12361 agtcgccctg ccgaactcga cggcctgctc accgagcgtc cagtcgaagc ggttcttctt
    12421 agtaggaagc gcgagaccca ggtacttgtg cagggtgatc gaggcgccta ccgcctcgtg
    12481 cagccggacg agatccgtga cttccgccac atcccatccg ctctcctccc gcgttgccgc
    12541 aatcatcgcg tttccgccgc gggccgcgtg catcgcacgc agggactgca ggacattcgc
    12601 tgtggcctga tcagtccagt cctcgcgctg ctcgaccacc cctccggccg tgaggagacc
    12661 gacggaaatg tcgggctgca tgacgatcag gcggtagtcg ccctgcggcg gtgtgaagct
    12721 ggtatccgcg acctgccggg ttgtcgtgca ggcagtgact gacatgaccg ccgccgcgac
    12781 caccaggatc cgtcttgtat tcattataag ccccccaagc catcataact ggccgcatgc
    12841 caacagccca gcgtctcctg cctcgccagg attgcgccac cctgccgaca gtcgtagcgt
    12901 tggcgtatcc gtccagccat cgaggattgc acccatggcg acaacgatgc ccgtccccgc
    12961 cgcgaaggcg ccgacggccg agtaccgcgc gctcgcgcaa ctgctcgaac ctgccgacgt
    13021 cgcgctcggc ggcacgcggc cctgggacat caccgtacac gatccgcgta tgcccgcacg
    13081 cgtgctcgcg cagggcgcgc tcggcgcggg cgagtcctac atggacggct ggtgggactg
    13141 cgagcggctg gacgaaatgc tgacccgcgt gctttgcctg ggagtcgacc ggcgcctgcg
    13201 ctcgatgcgc gtgctggcga ccgcgctgct cgcgagactg cgcaatccgc agagcccgcg
    13261 acgggcttac atcgttggcc gctcgcacta cgacatcggc gacgacctct acgagcgcat
    13321 gctcgatgca cgcatgatct acagctgcgc ctggtggctg cgcgcagcga acctcgatga
    13381 agcgcaggag cacaagctcg atctcgtgtg ccgcaagctc ggcctcgagc ccggcatgca
    13441 ggtgctcgac atcggctgcg gctggggcgg cgcggcgctg ttcgccgccg agcgctatgg
    13501 cgtcacggtg accggcatca cggtgtcgga acaccaggct gcagccgcgc gcctgcgctg
    13561 cagtgacctg ccggtcgaga tccgcctgga cgactaccgc cggctcaccg gccgctacga
    13621 ccgcatctgg tcgctcggca tgttcgagca tgtcggtgcg cgcaactacc gcacctattt
    13681 cgacaaggtg cgcgaactgc tcgcgcccga tggtctcttc ctgctgcaca cgatcggctc
    13741 gaaccgctcg gtgaccgtca ccgatccgtg gatcgagaag tacatctttc cgaactcgat
    13801 gctgccctcg atcgcccaga tcgggcaggc cgccgagccc cgctgggtgc tcgaggactg
    13861 gcatggcttc ggccccgact atgaccgcac cctgctcgag tggcatcgcc gcttcgaagc
    13921 cggctggtcg cagatcgcgg cgaaatacgg cgagcgtttc cgccgcatgt ggagattctg
    13981 gttgctgagt tccgccgctt cgttccgagc gcgccgggcc cagctctggc agttcgtgta
    14041 ctcgccatcg ggcaggccgg gcggttacca ggcggtgcgc tgatggccgt cgcggccccg
    14101 ttcgtgcaga ccgcgccagc cacaccgcgc gcttggtcgg acgactggct gttgcgcagc
    14161 gtgctgcgcc atcgcctgcc accggaggtg ctgctggcga tcagccccgc gctgctcgag
    14221 atggaagcgc ttgccgcagg cccgcttgca caactgcaga tggcagatcg cgagcgcgag
    14281 cctgtccaca caccctggga cgcctggggc aaccgcatcg atcgcatcga ggccacgccg
    14341 ctgtggcaag ccgccgagcc ccttgccgcc cggcacgggc tcgtcgccat tccctacgag
    14401 aagcagcatg ggcgttgctc gcgcatgcac cagttcgcgc tcgtgcacct gttccatccc
    14461 gtctcggaca tgtacacctg cccgctcgcg atgaccgacg gcgcggcgcg cgcgctgctc
    14521 gaatcggaca atgcggcgct gatcgagcgc gcattgccgc atctcacgag ccgcgacccg
    14581 gcgtccttct ggacgagcgg gcagtggatg accgaatcga ccggcggatc ggacgtgggc
    14641 gcctcgcaga gccgcgccgt gcgcacgagc gacggtagct ggcgcctcgc cggcaagaaa
    14701 tggttcacat cggccgcgac ctcgcagatg gcgctcacgc tggcgcggcc ggagggcaac
    14761 ggcccgggcg gtagcgggct cgcgatgttc tacctggaga cacgcgatac cgacggccgg
    14821 ctgaacggta tacgcatcga gcgcctgaag gacaaactcg gcacgcgcaa agtgcccacc
    14881 gccgagctca cgctggacga cgcgcatgca cagctcgtgg ctggtacgtc gcacggcact
    14941 cgcacgatcg agcccatgct gcgcatcacg cgcgcctgga acagcgtctg cgcggcgtcg
    15001 ctcatgcgcc acggatacct tctggcgcgc gactatgcgg cgaagcgccg cgccttcggc
    15061 gtgcgcctca tcgagctgcc actgcatgcc gagacgctcg cggacctcga cgccgagagc
    15121 gcgggtgcgc tgctgctcac gttcgagctg atcgacctgc tcggccggca ggaaagccac
    15181 gagctcgacg atgccggtgc ggcactgctg cggctgctga ccccggtcac caaggccgtc
    15241 acgggccggc aggcggtggc cgccgcgagt gaaacgctcg aggcgttcgg cggcgccggc
    15301 tacctcgaag acaccctgct gccggcgctg ctgcgcgatg cacaggtcgt gccctcccag
    15361 atcggcagga cctgtgcatc gcgcatggcc gatcccgcgc tgcgggcagc gaccgagacc
    15421 gcggtgcgcg ccgtgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcgca
    15541 tggccgatcc cgcgctgcgg gcagcgaccg agaccgcggt gcgcgccgtg gaccggggca
    15601 tcgcctggca gcgcaccgcc gccgacgacg aacgcgcatt gcaggcgggc gcccgacggc
    15661 tcgtgctctc gctcggccgc gcgttcgagc tcgccctgct cgcggagctt gcggatgccg
    15721 cggccatcga gggcgacgat cgcagcgccg cccgccgcgc ctgtctgcga ttgcaggcgg
    15781 ccggctactt cttcgtgtag gcaatctcca tctccgcctc gctcctggcc tcgtgccagc
    15841 tcttcatctc gaggtcacaa ccaagccgcg agccgtcaaa cccggacctc agctcgttgc
    15901 cgaaacgggg gtgtggttgc cacaggacgc ttgagcatat cggacgctct acgctcagcg
    15961 tatgttgccg ccgtcgacgg ggagatatat gccggtgatg gcgctgctcg ctggatcggc
    16021 gaggaacagc acgccccggg cgatatcgtc aattgtcggg gtcgatgctc ttccatccgc
    16081 tcgcggtatt cggtcagcac gtcctgccag tttcgtccgg cgcgttgtcg aggaaagccg
    16141 ccacggcatc cgcccaggct tccggctgct ggtcgacgat gtccaccccg ccgccttcga
    16201 gcgctgcaaa ggcgaaatcc ggccgcagag cctgcgcccg cagcgcgtga tcgtagatca
    16261 ggtcgccggt attcgtgaga atgagcgtcg gctgctggat cttgggcagg cgcgcggcct
    16321 gatcgtacat ataggcagcg tgatgaccgt accagaaggc gccgcgtccg atcagcgcct
    16381 gcacgacata gtcgctgagt cgcgccggag gaatcgagcc accggccatg cggtcgcgga
    16441 tatcgaacaa ttcgaccata tgggccgcat gggggcgggc cacataaccg agctcccatt
    16501 ggtgcaggtt ctggcggaac ttgtcccgtt cctcctcggt caccagcaga gggccgttga
    16561 tgatcagcgc gctgacccgc tggggataaa gaatcgcggc ctcggtcgcc gccagggcac
    16621 cggtgtggtg ccccagcacg gcggcgcgtt ccagcccgag atggtcgagt accgcgggaa
    16681 cgacccgggc gtagtcgccg accgtgggcg tatcgggcgt cggatccgac atcccgaagc
    16741 cgggcatgtc gatggcgatg gcgcggaacc cgcgacggac gagcggcacc atgacattgt
    16801 cgaactggcc cgatgccatc ggcgcctggt gcagaaggat gagcggccgt ccctctccca
    16861 gcacctggaa atgggcctgg ccgaaggggc cgtccgcata ggcgcgccgc ggcctgcccg
    16921 gtgctgcgtc tgtctgttca gatgtcgcca cgcgccactc tttccagtgt atcgacacag
    16981 gggcgcttgc cgcgtactgc actcgacggg aaacccggtt ctgtcatcac cctacctcgc
    17041 attcaaactc atcgagcgcc gccagcacct cggcgggcgg cttgacgtcc gcatacttat
    17101 aacccatgtc gaacagactc gcggcacggg cggccgggct gcagtcctgg acacatactt
    17161 ccactacgat gacacggaaa ttgagggaga agccttcgac acttacgtgc tggcggcaat
    17221 cgtccgcaag ctgttgaagc tggacgctac gatgcacgag actctgcaga tcctgaactt
    17281 gagcctgttc aggaaacacc cgctccatga actacttgcg ctctccgtgc agacaagtca
    17341 ccgagcagcg gcagcaccat cgcgtgacac gcttccgttc gatggccagc caggtcaggg
    17401 tgaccgcaca ccaggcgagg cacagccaga cgaacagccc cagcttgccc atgcggcgcg
    17461 gagcttctgc ggagaggcgc tgtcggtccg ctccatattc atatccatga cagattggcc
    17521 gcgtcatgcc ccacccgccg gtcggagtgg ggcggcgaca atgttcgaga cagggagatt
    17581 tcttcatgac caacattgct gagccagttt tcctggtcac cggatcggcg tccggcatcg
    17641 gcaaggcgat tgctgaccgg ctcacggtcg cggggcatcg ggtccgccgc ctcgacaaga
    17701 gcttcgacgc caccagcggc gcgggtatcg cgctcgacat aacggacgag gcggcgatcg
    17761 cgcacgccgt ggcggaaatt ggcgttgacg gacccatcgg ggcgctggtc aattgcgccg
    17821 gcatcggtgg actgggcaca gtgaccgaca tatccatgga gcattgggac agggtgatgg
    17881 cggtcaatct gcggggcaca gtgctgatgt gccgcgcggt gctgcccggc atgatcgggc
    17941 gcggccgcgg ctcgatcgtc aacgtcggct ccaccttcgg cctggtcgcc cgtggcggtt
    18001 gcgtcgctta tggggtcagc aaagccgccg tgatccatct cacccggtcg atggcggtcg
    18061 acttggctga cagcggcgtg agagtgaact gcgtctgccc cgggttgatc gagacgccaa
    18121 tgaccgagtc actgttcagg cccggcgagg aagaacggcg gcgggccaat atcgaggccc
    18181 atgcgctgcg ccgccctggg aagccggacg aagtcgccac ggcagtcgaa tatctcctgt
    18241 cggacgccgc gtccttcgtg accggcgccg tgctgccggt cgatggcggc tataccgccg
    18301 gaaagtgggt ctgaaagacg gcgagcgtcg cgcctacccg gacggaatca ggcgaccgcg
    18361 aggatgaggg ccgtgaggcc gaggctgcag gccgcagtcg ccacggcaat caccaaggca
    18421 gcggcgaatg gccgcattcc cacctggccc aggttcgcga gcgacaggtt gagaccgaca
    18481 gcggtcatgc cgcagatcat gaacagctcc gacagcgtct gcgcgacatc gagtgtcgca
    18541 ccccagccgc cggcaagtgc tgcgcgatcg gcgacgaact ggtcgccgag cgcgcgtacg
    18601 acgacgagcg cgacgaaggc cacgatgaac cacggcacca gcggccggcg gcgcgccgac
    18661 gaggccgtat ccggctgccg cgtcgtctcc gcatgggcga gccagccgaa gagcggaacg
    18721 agcacgatga tcgacaggtt gcgcagcagc ttcgtcgcgc tcgcgacggc cacggcatcc
    18781 ggcgaagcga actgctgcga atagatgagc gatgcgccga tcacctgcga ggtgtcatgg
    18841 atcgccgagc cgaggaacac acccgcaggc aacggatcgg taccgaatac cagcgccgcg
    18901 atccacggat acagcagcat gccggtgctg ccgatcagca cgacgcaggt gagcgccgtg
    18961 ccggtatcga tcgcgcgcgc gcgaatcacc ggcgcggtcg ccacgatcgc cgtgcagccg
    19021 cacaccgctg agccgacggc cagcagcctg cggagttcgg ctgacaggcc gaacgcgcga
    19081 ccgacgagct ggctgacggc gagcgcgacc aggatgcaga cgacgacgac cggaatcgcc
    19141 acgcggccga cgccggcgag cccgggcagc gtcagccgca agccgacgag ggcgatgccg
    19201 accagcagca gcgtgccgag gatccagcgc acccccgctt cggtgcgcgc accgacgccg
    19261 atgaaatggc gccacagcac gccgattacg atgccgacga gtaccgggct caccggtgag
    19321 ggccggccgt tggcaagtgc cgcgccgatt tcctgcgtgc cgattttcgc ggtaagcgcg
    19381 agcgcgagcg cgagtgccgc gcccggcagc actgcaagcc aggcgcccgc cttgccaggc
    19441 acggtcattc agcgcggact ggatgcgcca agggaatcag gcgtggaaga caactgcctc
    19501 agccgcgagc gcttggtgac catgcgctcg tgcaggcggg tcaggcagtc gctggccgtg
    19561 ttcgtgaaca ggaacggcag cgccgcgtgg atgaggcagg cgaagccggc cgtcatcatg
    19621 gacaggccac aacgccccgc gacgcccatg tgctggaggt aggtctcccc taccgagcgc
    19681 ggatgttccg tgaaggcttt atggatattc actgccgaga ctttagacgt tcgggtgcgc
    19741 aacaggattg caaaatactg gactgccgag ggcctgatga cgatactgtt tctcattgca
    19801 tggatttcgc gcaacaaatg gccatggacc gcatagacaa ggctattctg gacctcctgc
    19861 agcgcgacgc ctcgctgggc gtggccgacc tcgccgagaa ggtcggactg tcgaaggcgc
    19921 cctgctggcg acgcatccgc cgcctgcagg agcgcggcgt gatccgcggc accgtggcgc
    19981 tgctcgatcc caagttgctg aacgtcggca ccacggtgtt cgtcacgatc aagaccgcga
    20041 accacagcga ggcctggtac gagcgcttcc tgcagacgct cgacagcatc cccgaggtcg
    20101 tcgagatcta ccgcatgagc ggcgatgtgg actatctgct gcgcgtggtg gtgcccgaca
    20161 tcgaaggcta cggcgccgtg tatgcgcagc tgatcgccgg ctgcgaattc ctcgacatca
    20221 gcgcgagctt cgcgatgggc tcgatcaggc acaccaccgc gctaccgctg aaccacctcg
    20281 cccccggctg agcccgcctt gttgcgtgcg cgatagatgc tcgcgcgcat cagcagcatc
    20341 gcggtggccg gcgcggtcat caccacgaac acggtgatca ggaactcgtg cagcaccgga
    20401 cgcccggcga tcgccgagga gacgaacatc gaggcgaaca gcatgcagcc gcagccgagc
    20461 gtcgcgccca tcgacggtcc gtgcatgcgc gcctggaagc tcggcaggcg cagcagcccg
    20521 agcgatccga tcgccgtgag caggccgctc gcgatgagca gcaacgcggc gggcacggcg
    20581 gcccacatcg gcagctcgac cccgctcatg gctcgatcac ctcgccgcgc aacaggaact
    20641 tcgcgagcgc gaccgacccg acgaagccga acagcgcgat cagcagcgcg atctcgaagt
    20701 agaagccgga gccggagcgg atgccgagcg caagcagcat cagcatgccg ttgacgtaca
    20761 gcgtgtcgag cccgagcacg cgatcctggg ccgccggccc gcggatcacg cgccaggtcg
    20821 caaacagcat cgcgaggccg aagcagcccg acgcgaacca cgcggcgtaa tcgagcagtg
    20881 tcatggctgg aagatcccga gcagaggacg ctcgtagtgg tccttgatga tgcggatcca
    20941 gacctcctcg tcgcgcaggt cgagcacgtg cagcgtgagc tggccgcgct ccgcattgaa
    21001 gtcgacccag accgtgccgg gtgtggaggt gacgatcgtc gccagcaccg ccagcccgtg
    21061 cgggtcgcgc agttcgagcg gaatgtcgag gaaggcggag cggatccggc gctcgccgac
    21121 gagtccgagg atgatgcgcg cgaccgcgat gttcgagcgc acgatgtcga cgaataccgt
    21181 ccacagcaga ccgatcgcga gcagcgggcg gtacaggtgc ggccgcaagg gtcgcaactt
    21241 cgcggaccac agggtcagcg cgatcgctag accgatgccg agcacgacat gcccgggcga
    21301 gaccgtgtcg ttcagcagca gccacatcac cgcgagcgcg atggcgagca cgggcgcgaa
    21361 actcttcaca gcacctcctt gccggcgagc actgtttcga tatagatctg cggcgcgcgc
    21421 agcgacgcgg cggcctcatc caggaaggcc atcaccgggc ccgccgccac ggtcagcatg
    21481 atgcacagaa ccacgagcac ggccacggga ccggcctcga gcacgcgaag ccgcggcgtg
    21541 ttgcggccgg tcaccgacca gaaaagacgc atgccgatgc gcgtcagcgc cacgaggctc
    21601 gcgaggcctg tggcgagcgt gacgcccgcc agcacccaca gcggcccggc aagtgccgcg
    21661 tcgtcggcag cgtggaaggc cgccgacagc agcgcgaact tggcgacgaa tcccgacagc
    21721 ggcggcgagc ccgtcacgag cagcacgcag aaggcgaaca tgatgcccat gaaggccatc
    21781 gcggccggga tcgccacacc gacctcgtcg tcctgcgagt gcagcgccgg agtgcgacgc
    21841 acgccgaacg gcacatagtg cggcgggacc tccggctcgg gcgtggctgc ctgtggtgaa
    21901 gacccctgcg ccggcgagcg ccaggcccgc tcggtcattc cggccagcat gaagaacgcc
    21961 gccgtggcga gcaccgaacc gatcaggtag aacagcgccg gcgccatcag cgcgggaatg
    22021 tcgaggccga tcacggtgag cagcaacccg gacgaaacga tggccgagaa ggcaacgagg
    22081 cgcgcgaggt gctgcgcagc cagcatgccg aagatgccgt agcccatcgt caggatgccg
    22141 aggtaataca tgcctccgcg gaacagttcg gggcgcagca tggccgggcc ctcgatcgcg
    22201 gcgagcacgt cggcgccggc gccgagcgag ccgacacgca gcagcacgta gactccgacc
    22261 ttggtgagga tgccgaacac cgccgcgacc ggtgcggcgg cggcggcgta ggtgcccggc
    22321 agccagaagt tcagcggcca cgcgccggcc ttgatcagga acgcgaccgc gaggatcgag
    22381 gtcgcggcat cgaacagcgc gcgttccttg cccgcaagct gcgaggtcgc cgcgggcagt
    22441 tcggcgatgt tgaggctgcc ggtgacgccg tagacgagcg atacgccgat caggaacagc
    22501 agcgaaccca ccaggttgac gatgatgtag tgcaggctga ccttcacccg ctgcgtgctc
    22561 gaaccgtgca gcagcaggcc ataggacgcc gcgagcagta tttcgaagaa cacgaacagg
    22621 ttgaacaggt cgccggtcag gaacgcgccg ttgacgccca tcagcagcaa ctggaacagc
    22681 gtgtggaagt gcggcccgac gcgatcccag taggcgagtg aattgacgaa cgcggccagg
    22741 ccgacgaccg cggtcagcgt cagcatcatg ccggtgagtc gatcgatcac gaggacgatg
    22801 ccgaacggtg gctgccacgc gccgatcgcg tagacgccga caccccgcgt ccagacatcg
    22861 gggaaggcgc tggtcgtcag tgtcagtagc gcgatggccg cgcacagttg cacgaacatc
    22921 gacaggccgg caatggcgat gcgtatccgg cgcgagcgct cggtcaagaa caggatcgtc
    22981 gcagccgcga ccagcggcgc gacgatcggc accatgggca ggtgctgcag cagatccgtc
    23041 atggctcccg cccgtcgaca tggtcggtgc cggtaagtcc acgcgcggcg agcagcacca
    23101 ccagcagcag cgcggtggtc gcgaagccga tcacgatcgc ggtcagcacg agcgcctgcg
    23161 gcaccgggtc ggcatacaga gccacgtcgg tggcgtcggc gcccgcgaga atgggcgcgc
    23221 ggccgatcgt caggcgcccc ataccgaaaa tgaagaggtt gacggcatac gacagcagcg
    23281 cgagcccgat ga
//
LOCUS       DAOQVY010000029        23217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_2580_length_23217_cov_6.023875, whole genome shotgun sequence.
ACCESSION   DAOQVY010000029 DAOQVY010000000
VERSION     DAOQVY010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 23217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23217
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2580_length_23217_cov_6.023875"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..1863
                     /gene="aceK"
                     /locus_tag="PKN91_04440"
     CDS             133..1863
                     /gene="aceK"
                     /locus_tag="PKN91_04440"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HNS27354.1"
                     /translation="MSAEPALAVECARHIAAAFADYNAEFRSITRRAPQRFDARDWKG
                     SQRDAVERIELYDRWVDRAIAELRLKLGDRACDRELWREVRMHFARQIEGLPDSEFTK
                     TYFSSITRRLHGTVGVAPEIEFVAADLDPLASVTSEVVTNVYLHRGSLPLLFEDVLGD
                     LRFRSPWKDFDRSVAHVAAEVGAHLAALGERRVLTRIDMIRPVFYQLTRAFLVGRMSG
                     RGFTVPLVIALKNTESGMHVDAVMLAEADVSIVFGFTRSYFHVDLDRVGEAVVFLKAM
                     LPRKPVSELFTVLGRAKQGKTERFRELIRHMEQTSDKFVHAPGERGLVMVCFTLPSFD
                     VVFKVIRDRFPYPKNVLREEVLGKYNYVFKHDRAGRLVDAQEFKRLRFPRERFAPELL
                     EELLTETASTVHVEGEDVILDHLYIERRMTPLNLYLRTASPEAAELAVLDYGQAIRDL
                     AYTNIFAGDLLLKNFGVTRHGRVIFYDYDELCQVTDCHFRDMPQATNPEDEMRGESWF
                     FVADNDVFPETFINFLAFDEVQRAAFLRVHAEILTAEFWRSVQKRHIEGELMEVLPYH
                     VHRVRVASSA"
     gene            complement(1887..3059)
                     /locus_tag="PKN91_04445"
     CDS             complement(1887..3059)
                     /locus_tag="PKN91_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HNS27355.1"
                     /translation="MKKPTRVNHPPRVEMPADNRPLLTPIYQSVKFEFDTVEETVRGM
                     RGERPGFYYMRASNPTTRQLELTLAQLQDRDDCLACCSGVNAIVQTLLGLTKQGDHVL
                     CFVETYGPTRRIIRRLLGRFGVTHTMLSIEDLPGIEHTLATTATRLVIFESPTNPVTK
                     IADIAAITRLARQHGALTVMDNTFAGLHQHGEFDIDYYLHSLTKYASGAGDVMGGAVI
                     ARAELLRPLRAEFSMFGALLDPHAAFLIQRGLKTYFVRYREQSASAQRIAERLAQHPA
                     VARVHYPGLPTHPRHALARAQMREFGTIVSFDLRGGGEAGRRFAERLELFALTASLGS
                     TESLVMAPQMLESRDLSTDQRAISAVTEGTVRLSIGLEDIDDLLQDIERALAAAGG"
     gene            complement(3056..3844)
                     /locus_tag="PKN91_04450"
     CDS             complement(3056..3844)
                     /locus_tag="PKN91_04450"
                     /inference="COORDINATES: protein motif:HMM:NF007757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNS27356.1"
                     /translation="MRISLVQTTLDWRDPPANRARLAGLLAPLAGTTDVVLLPETFPT
                     GFDMEGAESAEPMGGASCQWLADQARALDAAVVGSVFIRDGAGVYNRLLWAMPDGLIT
                     HYDKRHLFRMAGEHRRFAAGSVPLAVDWRGARFCPLVCYDLRFPVWSRRRATLDYDVL
                     VYVANWPAPRHYAWQQLLRARAIENQAFVLGVNRVGPDGNGVAYSGGSAAIDALGEPL
                     VELGDRPETATVTLDLAAQRGLRERFPVERDADPFTLVAGGERR"
     gene            complement(3854..5017)
                     /locus_tag="PKN91_04455"
     CDS             complement(3854..5017)
                     /locus_tag="PKN91_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009485198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HNS27357.1"
                     /translation="MITSKLPDVGTTIFTVMSHRAAQLGALNIGQGFPDYPIDPKLAA
                     ALTEAVAAGHDQYAPMEGSSALREAIAAKLRASHELEVDPVDEITVTCGATEALYSAI
                     QAVVGPGDEAIVFDPAYDSYDPAIRLAGGRAIHIPLAPPRFRYDWERVRAAITPRTRL
                     LILNTPLNPACTVARAGDLAALAELLRERPIALLADEVYEHVIFDGRRHESALAHPEL
                     RAKSFVVFSFGKALHATGWRIGYCVAPRALTAELRKVHQYNTFSIAAPLQAAIARYLP
                     RHPESWQGLAAYFEAKRDRLVTALAGSGFGMQPAEGTYFQLLDFSAIAPAESDSAFAT
                     RLLEEGGVATIPLSPFYRDPPRLSFVRVCVAKLDATLDVAAERLRAFAGRPTR"
     gene            complement(5014..5751)
                     /locus_tag="PKN91_04460"
     CDS             complement(5014..5751)
                     /locus_tag="PKN91_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNS27358.1"
                     /translation="MARWYLGIDVGGSSIKAGLVDVDTGAMPGDAVTRITPRPATPAA
                     IADTIGEIAARLPGGEHVGVGIPSVVKHGKAWTAANIHESWVGVDVGALLSGALGRPA
                     TFLNDADAAGLAEMRFGAGRDVPGTVMVLTLGTGIGSAPFVDGRLLPNTEFGHLQLHG
                     ASAELYASARVRHEQGLGWPEWGARLTEYLGYMERLFWPDLFILCGGVIEHFGEFAPY
                     LSTRTPVVPALLGSSAGIVGAALAAAA"
     gene            complement(5759..6616)
                     /locus_tag="PKN91_04465"
     CDS             complement(5759..6616)
                     /locus_tag="PKN91_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNS27359.1"
                     /translation="MRLRGRHWFSKTGLWIGLAFLYIPIVSMIFFSFNNSRLVTVWDA
                     PNSPTLKWYGELFRNDQILGAAWLSVRIAAMTATGAVILGTLAGLVLARFGPFRGRAM
                     LSGMTTAPLVMPEVITGLSLLLLFVALQQLIGWPATRDATTITIAHITFSMAYVTIVV
                     QSRLAGFDNSLEEAALDLGAKPAKVFFRITLPLIVPAILSGWLLAFTLSWDDVVVSQF
                     VAGPGSTTLPMVVFSKVRLGVSPDVNALATIMVLIVAVGVAISAVWMRRQERRREREM
                     QMAAAANNK"
     gene            complement(6613..7614)
                     /locus_tag="PKN91_04470"
     CDS             complement(6613..7614)
                     /locus_tag="PKN91_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNS27360.1"
                     /translation="MPRLPPPIPSTRLLTGRLGPWWHLFFRFGPPRWTQYMRHNWAGK
                     RLVIAVPYLWLLLFFLVPFIIVFKISFSEVRLAMPPYAPLWEFVSDKAVDIRLNFGNY
                     LFLLTDDLYISSYLYSLKVAAISTVFCLLLAYPMAYAIARSTPATRNLYLMLIILPFW
                     TSFLLRVYAWIGLLKNNGVINNFLIWAGVIDTPITMMQTDFAVFIGIVYSYLPFMILP
                     LYSNLEKHDHTLLEAAADLGANPIVSFLRITLPLSVPGIVAGSLLVFIPAVGEYVIPS
                     LLGSSDQLMIGRVLSDEFFANRDWPVASAVAIAMLLFLMVPIMVFQRYQARELAGSK"
     gene            complement(7628..8773)
                     /gene="potA"
                     /locus_tag="PKN91_04475"
     CDS             complement(7628..8773)
                     /gene="potA"
                     /locus_tag="PKN91_04475"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HNS27361.1"
                     /translation="MAERRNDKKVEPWRDPSARAYVELQNVTKRFGDFVAVNNVSLKI
                     YQHEIFCLLGGSGCGKTTLLRMLAGFEEPSSGRILIDGKDMAETPPYERPTNMMFQSY
                     ALFPHMTVAKNVAFGLEQEKLPKAEIAQRVGEILEIVKMGEFAQRKPHQLSGGQRQRV
                     ALARSLAKRPKLLLLDEPLGALDKKLREQTQFELINIQERLGVTFVVVTHDQEEAMTL
                     ASRIGVMNRGEIVQIGTPSEIYEFPGTRFVADFIGSVNTFEGHLIEDLPDRVRIRSQE
                     LGGVVYVDHGISAAPGAIVWAAIRPEKINISRAAPADASENCVRGIVKEIAYMGDLSI
                     YLVQIESGKIVRVTMPNIERLSDDERILWDEQVYLSWHASSPVVLSQ"
     gene            complement(8822..9976)
                     /locus_tag="PKN91_04480"
     CDS             complement(8822..9976)
                     /locus_tag="PKN91_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HNS27362.1"
                     /translation="MSISLRLLVLGATAAFVVACGGKPSGEAAPAADAAAPGALDTDE
                     EKILNVYNWSDYIEESVIPEFEAETGIKVNYDVFDSNEVLETKLLAGSTGYDVVVPSA
                     SFLERQIKAGVFMPLDKSKIPNLVNADPVITERVALHDPGNEHSVNYMWGTSGVGYNV
                     EKIAAAMPDAPVDSWRMFYDPAVVSKFKDCGVTLLDAPSEVIGTVLIYLGRDANSEKP
                     EDLQAAEDVLMKIRPYVRYVNSSKYIDDLANGEICLALGWSGDVLQSRDRAAEAEKGI
                     TIAYNIPKEGAIAFFDNFAIPKDAKHVKNAYIFIDYMMRPEVAAKNTNYINFASNNAA
                     ALPMIDDSVKGDVSIYPTPEVMAKIVPDLAESAEFTRQLTRSWTRFKTGK"
     gene            complement(10040..11416)
                     /locus_tag="PKN91_04485"
     CDS             complement(10040..11416)
                     /locus_tag="PKN91_04485"
                     /inference="COORDINATES: protein motif:HMM:NF005682.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNS27363.1"
                     /translation="MPSPSTAELQQLDSAHYLHPFTDYKQLAARGARVVTSADGVWLT
                     DSDGNRILDGMAGLWCVALGYGRKELAEAAYRQMLELPYYNSFFQCATPPAIALAQRL
                     AEVTPPQFNRVFLTGSGSEANDTMVRLVRRYWQLRGEPGRNVIIGRWNGYHGSTVAGA
                     SLGGMQPMHEQGGLPIPGIVHINQPYWFERGGDLSPAEFGLRTARELADRIEEIGPRR
                     VAAFIGEPVQGAGGVIIPPETYWPEIQRICDHYGILLVSDEVICGFGRTGRWFGCEHF
                     GTRPDLMPIAKAMSSGYLPIGGLMVADRVAEVLIAEGGEFYHGFTYSGHPAACAVAVA
                     NIDIMRREQVVERVRDSLAAHFATRLRSLADHPLVGEVRTVGLLGALELVPEKPSRRF
                     FGNRGEVGTVCRDLCVANGVVSRAVRDTMICAPPLVITPAEIDELVARLARALDQTAA
                     VIAREGWS"
     gene            complement(11449..12789)
                     /locus_tag="PKN91_04490"
     CDS             complement(11449..12789)
                     /locus_tag="PKN91_04490"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNS27364.1"
                     /translation="MAGEIQQFFRDHGISEVEAIIPDMAGIARGKVMPAAKFAEEGGM
                     RLPESIFLQTVTGDYPIHPETAMSPAEIDIVLRADPKTVRRVPWTAEPTAQVIHDCFY
                     ADGRRVTMAPRHVLRQIIELYAERGWEAVVAPELEFYLVEPNADADYQLKPPIGRSGR
                     SEPGRQSYSIAAVNEFDPMFDDMYQFCEDQDIEIDTLIHEDGPAQMEINLLHGNPLDL
                     ADQAFLFKRTAREAALRHKMYATFMAKPHAKEPGSAMHIHQSIVDRKSGRNVFSNEDG
                     TPSKLFFSHIAGLQKYLPFAMALLAPNVNSYRRITRFQTAPINVQWGYDNRTAGLRVP
                     MSDPASRRIENRLSGADANPYIAIAASLACGYLGMIEGLEPSEPIAVSAHDLPFQLPR
                     SLDDGIRKLRECAPLIRVLGDKFVNAFTIVKESEYEVFLQVISSWEREHLLLNV"
     gene            complement(12793..13554)
                     /locus_tag="PKN91_04495"
     CDS             complement(12793..13554)
                     /locus_tag="PKN91_04495"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNS27365.1"
                     /translation="MAKRRPVIGIPADRRMLGAHPFHAVGEKYIRAVVDAADALPLLV
                     PALGAEIDAGELFASIDGLLFTGSPSNVEPHHYSGPASVPGTEHDAARDATTLPLIPL
                     AVGQGIPVLGLCRGFQEMNVAYGGTLWQRVQEQQGYMDHREDKTQPLEVQYAPAHDVE
                     LIAGGVLRSLTDSDRIRVNSLHSQGVQRLGPGLAIEARAPDGLIEGFRVEDAPAFALA
                     VQWHPEWQVMGNSFSRALFAAFGAASAVRAQGRQR"
     gene            13780..15150
                     /locus_tag="PKN91_04500"
     CDS             13780..15150
                     /locus_tag="PKN91_04500"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNS27366.1"
                     /translation="MSRETRPELQRFLEAYPDTRHFDVFLHDLNTVERGKRLDLAGME
                     AAYRGGMLLPGSMFALDVLGHTVEATGLGFDDGDADRPCRPIPGTLVPVPWQGAHAAQ
                     VQVTMHEPDGRVFYGDPRHVLTGVLQRIEALGLTPVVAIEYEFYFIDLERVDGGLPQP
                     PRGPLTGRREFRTQINAMADLNEYSSVLAQITRDCHAQGVPATSALPEYGPGQYEVNL
                     QHLPDALTACDQALRFKRVVKSVARANGLDATFLPKPYRDMAGSGLHIHLSLLDGEGR
                     NIFACDEPATHAPLRHAVAGLLDSMAEGMAICAPGPNSYRRFRSEAYVPLTTSWSINN
                     RGSAVRIPVSDAANRRIEYRQAGADANPYLVLAWMLAGIHHGLGARIEPPEPLRGNAY
                     AQPAGQPLPTHWETAIERFAGSDFAREYLGERFQRLFATVKRAELADFGSYVTPLECA
                     WYMGPL"
     gene            15157..16437
                     /locus_tag="PKN91_04505"
     CDS             15157..16437
                     /locus_tag="PKN91_04505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNS27367.1"
                     /translation="MSTSEYVDSYYAASRHPAPSHAPLAGDVDCDVCVVGGGIAGCSA
                     ALHLAERGYRVVLLEAARIGWGASGRSGAQALFGLAAGEEKTEALVGREDARRIWDMT
                     VEGLALMRGLIARHAIDCDYVPGQMHVAVRPRQVGELRNWVDLIHARYDYRSLRFVPR
                     EELRDLIASERYLAGTFDSNSAHLHPLNYTLGIAAAAGRAGVRIHEGSRALDYEAGEV
                     VTVRTAQGRVRCRHLALCGNAWLGDTAPAIARRIMPVGTYIVATEPLGLERARALIAN
                     DAAVTDINWILDYFRLSRDHRLLFGGRVSYSGLDPFNTAQATRQRMLRVFPQLADARI
                     AYSWGGYVDITLNRAPDFGRLAPNVYYLQGFSGHGIVLTGIAGKLLAEAVAGTAERFD
                     VFARIRHREFPGGALLRRPALVLAMAWYRLRDLL"
     gene            complement(16442..18610)
                     /gene="rlmKL"
                     /locus_tag="PKN91_04510"
     CDS             complement(16442..18610)
                     /gene="rlmKL"
                     /locus_tag="PKN91_04510"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: protein motif:HMM:NF008748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HNS27368.1"
                     /translation="MRFLASAPRGFGDLLAAEITLLGGTDVRERLLGVSFSGTLEVAY
                     RACLESRLASRVFLEVAQVEAASADELHAALHALPWGEHVDPDGTLACDYSGQHPGIN
                     HTRYGAQRVKDAICDALRERYGRRPDVSLERPSVRVHAHAHGSKVTVSIDLSGEGLHR
                     RGYRSQAGEAPLRENVAAGILVRAGWRQLVSEGAELLDPMCGSGTFVIEGAMIAADIA
                     PGLAREYFGFTGWKGHDAALWQRLRAEAQSRVRPHDGGVPRLRGSDIDERMVRLAAEN
                     AARAGVADWVQFTRARLADARPNAARGLLCVNPPYGVRLEDRETARALHRELGAVLRE
                     HFNGWRAAVITGAPEMGLEIGLRAARTHTVWNGALECRLLRIEVSPQSVRDFTSPRPA
                     RLADPALRDTPGARMFANRLGKNAKRLGDWATRAGIGCYRVYDADMPEYAFAIDLYTA
                     ADSPMRWAYVQEYAAPREIELEAVRRRRGEALSVLPEVLGLPESQVMVRMRRRTGRGE
                     QYEKEAERGEFHVVAESGLRFRVNFTDYLDTGLFLDLRLTRARLREAAAGGRFLNLFA
                     YTGSATVYAAAGGARATTSVDLSRTYLEWAQQNLALNGLAGRQHRFVQADCREWLGEA
                     ARLGERFDLIFLDPPTFSNSKRMQGVHDVQRDHPALIDACMRLLAPGGLLVFSTNAQK
                     FRIDQGLAERYALTDVTRATIPADFARNPRIHTCFEIRAR"
     gene            complement(18610..20022)
                     /locus_tag="PKN91_04515"
     CDS             complement(18610..20022)
                     /locus_tag="PKN91_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNS27369.1"
                     /translation="MTALLDAPLAFVDVETTGGHPLYHRIIEIGVVLAQGEKIEESWT
                     TLVNPGTWLPPTIQRLTGIDPGSLADAPPFRAIADELATRLEGRLFIAHNARFDYGFV
                     RNELKRAGHAFDARLACTVKLSRRFYPEMAHHNLDALIAHHELACAARHRALPDAMAL
                     WQFWRVLRASWPPARLEAALAEVTRRTPIPPQLPPELADELPQGAGVYRFYGEGDAPL
                     YVGKAKNLRERVLAHWGGAPRSLKARRLSTQTTRVEWTETAGELGAMLLEARCVRESL
                     PVYNRRLRGGGEALSWQFEDDAATPRLVSLDDGWPAGDCFGIYRTTREATSALEHLAR
                     EHGLCARVLGLEGGEGSCFAYQLQRCRGACVGAESPQRHLARSKLAFAPQRLKPWPYE
                     GAVAVHERAFGGFEQWHVIDGWRYLGTVTRHEGVEDLPPEPELAVQGPAVGFDVDVYR
                     ALTGFLAARPRGVVPWPPRA"
     gene            complement(20019..21518)
                     /locus_tag="PKN91_04520"
     CDS             complement(20019..21518)
                     /locus_tag="PKN91_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNS27370.1"
                     /translation="MSAAKPVIDWHARAAQLTAEGRAFIDGDYVGALSGEQFDNVDPA
                     TGKVFGRVAAGDTADIERAVASARAAFRSGVWSQTRPSHRKKVLAKFAQLIQGHREEL
                     ALLETLDMGKPISDSLKIDIPGAARCIEWYAEAIDKIYDEVAPTGPDSLALITREPMG
                     VVGAIVPWNFPLAMAAWKIGPVLAAGNSLVLKPSEKSPLSAIRIAQLGIEAGLPPGVF
                     NVVPGFGHTAGKALALSMDVDAITFTGSTATGKLVMQYAAQSNMKRVSLECGGKSPNI
                     VLADYPDIERAATAAAYAIFFNQGEMCSAGSRLLVQRTVRDAVLERVQAIGRTMQPGD
                     PLDPATTLGAIVDEVQMKRVLGYIDTGRKEGAKVAFGGRRVREDSGGYFVEPTVFDGV
                     RREMRIAREEIFGPVLATITFDTLEEAVEIANDVDYGLAAAVWTRDVRTAHRVARALR
                     AGVVYVNCYDADDITVPFGGFKQSGFGRDKSLHAFDKYTELKTTWLDLS"
     gene            complement(21530..22078)
                     /locus_tag="PKN91_04525"
     CDS             complement(21530..22078)
                     /locus_tag="PKN91_04525"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNS27371.1"
                     /translation="MSIDVGARLRSVRLIYGLSQRELARRAGVTNGLISLIEQNRVSP
                     SVGSLKKVLDGVPMSLAEFFTMNVDAPAQIFYGDDELIEIGTEDVSLRLVAAQRPARQ
                     VTILHERYAAGAATGDDALAHRGEEGGIVIRGRIELTVGGSTRVLGPGEAYYFTSSIP
                     HRFRNVGREPCEIISASAPSTF"
     gene            22166..>23217
                     /locus_tag="PKN91_04530"
     CDS             22166..>23217
                     /locus_tag="PKN91_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNS27372.1"
                     /translation="MSTSHTSLDALWLPFTANRQFKQAPRLLARASGMEYFTPEGRRV
                     LDGAAGLWCVNAGHGRKEITAAVAQQLETLDYAPTFQMGHERAFELATRLAELTPAGL
                     DRIFFTNSGSESVDTALKIALAYHRVRGDAARTRLIGRERGYHGVGFGGITVGGIVAN
                     RKAWSASLIPGADHLPHTHDLARNAFSRGLPEHGAELADELERLVALHDASTIAAVIV
                     EPVAGSTGVLLPPKGYLERLRAICDRHGILLIFDEVITGFGRTGSPFAAQEFGVTPDI
                     ITMAKGLTNGCIPMGAVAVKQSIHDAFMQGPPGTIELFHGYTYSAHPAACAAGLATLD
                     IYQREGLLTRAKTLSKT"
BASE COUNT         3805 a         7968 c         7787 g         3657 t
ORIGIN      
        1 ggatcgcacg atcggccaga agaccgtgac ctacgatttc gcgcgcctga tggagggcgc
       61 caccgaggtc aagtgcagcg agttcgccga cgcgctgatc aagaacctct gagcggtccg
      121 ggtgcgcccc gcgtgagtgc ggaaccagcg ctcgcggtcg aatgcgcgcg gcacatcgcc
      181 gccgcattcg ccgattacaa cgccgagttc cgctcgatca cgcggcgcgc accgcagcgc
      241 ttcgatgcac gcgactggaa gggcagccag cgcgatgcgg tcgagcgcat cgagctctat
      301 gaccgctggg tcgaccgcgc gatcgccgag ctgcgactga agctcggcga tcgcgcctgc
      361 gaccgggagt tgtggcgtga agtgcgcatg cacttcgcca ggcagatcga gggcctgccc
      421 gactccgagt tcaccaagac ctatttcagc tcgatcacgc gccggctgca cggcacggtc
      481 ggtgtcgccc cggagatcga gttcgtcgcc gccgacctcg acccgctcgc gagcgtgacg
      541 agcgaagtcg tcaccaatgt ctatctgcat cgcggctccc tgccgctgct gttcgaggac
      601 gtgctcggcg acctgcgctt ccgctcgcca tggaaggact tcgaccgcag cgtcgcccac
      661 gtcgccgccg aagtcggcgc gcatctcgcg gcgctcggcg agcggcgtgt cctgacgcgc
      721 atcgacatga tccgacccgt gttctaccag ctgacccgcg cgtttctcgt cgggcgcatg
      781 agcggacgcg gcttcacggt accgctggtc atcgccctga agaacaccga aagcggcatg
      841 cacgtggatg cggtgatgct ggccgaggcc gacgtcagca tcgtattcgg cttcacgcgc
      901 tcctacttcc atgtcgacct ggaccgcgtg ggcgaggcgg tcgtgttcct gaaggccatg
      961 ctgccgcgca agccggtcag cgaactgttc accgtgctcg ggcgcgccaa gcagggcaag
     1021 accgagcgct ttcgcgaact gattcggcac atggagcaga cctcggacaa gttcgtgcac
     1081 gctccgggcg aacgcggcct cgtgatggtg tgtttcacgc tgccctcgtt cgatgtcgtc
     1141 ttcaaggtga tccgcgaccg cttcccgtac ccgaagaatg tgctgcgcga ggaggtgctc
     1201 gggaagtaca actacgtctt caagcacgat cgtgcgggcc gcctcgtcga cgcgcaggag
     1261 ttcaagcggc tgcgcttccc ccgcgagcgt ttcgcgcccg agctgctcga ggagctgctg
     1321 accgagaccg ccagcaccgt gcacgtggag ggcgaggacg tgatcctcga ccacctgtac
     1381 atcgagcggc gcatgacgcc gctcaacctc tacctgcgca ccgcgtcacc cgaggccgcg
     1441 gagctcgcgg tgctcgacta cggccaggcg atccgcgatc ttgcctacac gaatatcttc
     1501 gccggcgacc tgctgctgaa gaatttcggt gtcacccgcc acggccgcgt gatcttctac
     1561 gactacgacg agctgtgcca ggtcaccgac tgccacttcc gggacatgcc gcaggccacc
     1621 aacccggagg acgagatgcg cggcgagagc tggttctttg tcgccgacaa cgacgtgttt
     1681 ccggagacct tcatcaactt cctcgccttc gacgaggtgc agcgcgcggc cttcctgcgg
     1741 gtgcacgccg agatcctgac tgccgaattc tggcgcagcg tgcagaagcg ccacatcgag
     1801 ggtgagctga tggaggtgct gccttaccac gtgcatcgcg tgcgggtggc gagcagcgcc
     1861 tgaaccgtgc cgcacaccgg gtcggctcag ccccccgccg cagccagtgc gcgttcgatg
     1921 tcctgcagca ggtcgtcgat gtcctccagc ccgatcgaga ggcgcacggt gccttcggtg
     1981 accgccgaga tcgctcgctg atccgtgctc aggtcgcgcg actcgagcat ctgcggggcc
     2041 atcaccagcg attcggtcga cccgaggctc gcggtcagcg cgaacagctc gaggcgttcg
     2101 gcgaagcggc gccccgcctc gccgccaccg cgcaggtcga agctcactat cgtgccgaac
     2161 tcgcgcatct gggcgcgcgc gagcgcatgc cgagggtggg tcggcagccc cggatagtgc
     2221 acccgcgcga ccgcgggatg ctgcgcgagc cgctcggcga tgcgctgcgc gctggcgctc
     2281 tgttcgcgat agcgcacgaa ataggtcttc agcccgcgct ggatcaggaa cgccgcgtgc
     2341 ggatcgagca gtgctccgaa catcgagaac tccgcgcgca gcggacgcag cagctcggcg
     2401 cgcgcgatca cggcgccgcc catcacgtcg cccgcacccg aggcgtactt ggtgaggctg
     2461 tgcaggtagt agtcgatgtc gaattcgccg tgctggtgca ggccggcgaa agtgttgtcc
     2521 atcacggtga gcgcaccatg ctgacgcgcg agccgcgtga tggcagcgat atcggcgatc
     2581 ttcgtgaccg gattggtcgg cgactcgaag atcacgagcc gcgtcgcggt ggtcgcgagc
     2641 gtgtgttcga tgcccggcag gtcctcgatc gagagcatcg tgtgcgtgac gccgaagcgg
     2701 ccgagcaggc gccggatgat ccgccgggtc gggccgtagg tctcgacgaa gcacagcacg
     2761 tggtcaccct gcttcgtcag gccgagcagc gtctggacga tggcgttgac gcccgagcaa
     2821 cacgcgaggc aatcgtcccg gtcctgcaac tgcgcgagcg tgagctcgag ctggcgcgtg
     2881 gtcggattcg aggcgcgcat gtaataaaag cccggccgct cgccgcgcat gccgcgcacg
     2941 gtctcctcga ccgtgtcgaa ctcgaacttc accgactggt agatcggcgt caggagcgga
     3001 cggttgtccg cgggcatctc cacccggggc ggatgattga cgcgcgtggg cttcttcatc
     3061 ggcgctcgcc gccggcgacc agcgtgaagg gatcggcatc gcgctcgacc gggaagcgtt
     3121 cgcgcagccc gcgttgcgcg gccagatcga gcgtgacggt cgcggtttcc ggccgatcgc
     3181 ccagttccac caggggctcg ccgagtgcat cgatcgcggc acttccgccc gagtaggcga
     3241 cgccgttgcc gtccgggccc acgcggttca cgccgaggac gaaggcctgg ttctcgatcg
     3301 cgcgggcgcg cagcaactgc tgccaagcgt agtggcgcgg cgcgggccag ttcgcgacat
     3361 agacgaggac atcgtagtcg agtgtcgcgc gtctgcggct ccagacgggg aaacgcaggt
     3421 catagcacac gagcgggcag aaccgcgccc cacgccaatc cacggcgagc ggaacgctgc
     3481 cggccgcgaa tcgcctgtgc tcgcccgcca tgcgaaagag atggcgcttg tcgtagtgtg
     3541 tgatgagccc atcgggcatc gcccacagca ggcggttgta cacgcccgcg ccgtcgcgga
     3601 tgaagacgct gccgacgacc gccgcatcga gtgcgcgcgc ctggtcggcg agccactggc
     3661 agctcgcgcc gcccatgggc tcggcgctct cggcgccttc catgtcgaac ccggtcggaa
     3721 aagtctccgg caggagcacg acatcggtcg tgccggccag tggcgcgagc aagccggcga
     3781 ggcgggcgcg gttcgccggc ggatcgcgcc agtcgagcgt cgtctgcacc agggagatgc
     3841 gcatgtgcgc agtctaccgc gtggggcggc cggcgaacgc gcgcagtcgt tctgcggcaa
     3901 cgtccagtgt cgcgtcgagc ttggcgacac agacccgcac gaagctgagg cgcggtggat
     3961 cgcggtagaa cggcgacagc ggaatggtcg ccacgccccc ctcctccagc agccgggtcg
     4021 caaacgcgga gtcggactcg gcaggcgcga tcgcgctgaa atcgagcaac tggaagtagg
     4081 tgccctccgc cggctgcatt ccgaagccgc tgcccgcaag cgccgtgacg aggcggtcgc
     4141 gcttcgcctc gaaatacgcc gcgagtccct gccagctctc cggatgtctc ggcaggtacc
     4201 tcgcgatggc cgcctgcagg ggagccgcga tgctgaaggt gttgtactgg tggactttgc
     4261 gcaactcggc cgtcagcgcg cgcggcgcga cgcagtatcc gatgcgccag ccggtcgcat
     4321 gcagggcctt gccgaaggag aagacgacga agcttttcgc gcgcagttcg gggtgggcga
     4381 gcgccgactc gtggcgacgg ccgtcgaaga tcacgtgctc atagacctcg tccgcgagca
     4441 gcgcgatcgg gcgctcgcgc agcagctccg cgagcgcggc gagatcgccg gcacgtgcca
     4501 ccgtacacgc cggattgagc ggagtgttca ggatcagcag ccgcgtgcgc ggcgttatgg
     4561 cggcgcgcac gcgctcccag tcgtagcgaa agcgcggcgg tgccagcgga atgtggatcg
     4621 cgcgcccacc ggcaaggcgg attgccggat cgtaggaatc gtaggcgggg tcgaacacga
     4681 tcgcctcgtc gcccgggccc accacggcct ggatcgccga atacagtgct tcggtcgcgc
     4741 cgcaggtcac ggtgatctcg tccaccggat cgacttcgag ctcgtggctc gcacgcagct
     4801 tggcggcaat cgcctcgcgc agggcgctcg agccctccat cggcgcgtac tggtcgtggc
     4861 ccgccgcgac cgcctcggtg agcgcagcgg ccagcttcgg gtcgatcggg tagtccggaa
     4921 agccctggcc gatgttcagc gcgccgagct gagcggcgcg atgcgacatc acggtgaaaa
     4981 tcgtcgtgcc gacgtcgggc agcttgctcg tgatcatgcg gccgcggcga gcgcggcgcc
     5041 gacgatgccc gcgctgctgc cgagcagcgc gggcacgacc ggcgtgcggg tcgacaggta
     5101 aggcgcgaac tcgccgaagt gctcgatgac accgccacac aggatgaaca gatccggcca
     5161 gaagaggcgc tccatgtacc ccaggtactc cgtcaatcgc gcgccccatt ccggccagcc
     5221 gagcccctgc tcgtgacgca cccgcgcgga cgcatacaac tccgccgagg cgccgtgcag
     5281 ctgcaggtgg ccgaattcgg tattcggcag cagcctgccg tcgacgaagg gcgccgagcc
     5341 gatgccggtg ccgagagtca gcaccatgac ggtgcccggc acgtcgcgac cggcaccgaa
     5401 gcgcatttcc gcgagcccgg ccgcatctgc atcattgaga aacgtcgccg ggcgcccgag
     5461 tgccccggac agcagtgccc cgacatcgac gccgacccag ctctcgtgga tgttggccgc
     5521 ggtccaggcc ttgccgtgct tcaccactga cgggatgccg acgccgacgt gctcgccgcc
     5581 cggcaatcgg gcggcgattt cgccgatcgt gtccgctatc gccgccggcg tggccggccg
     5641 cggcgtgatg cgggtcaccg catcgcccgg catggcgccg gtatcgacat cgaccaggcc
     5701 tgccttgatc gaagagccgc ccacatcgat gccgaggtac cagcgtgcca tgggctggct
     5761 acttgttgtt ggctgcggcc gccatctgca tctcacgctc gcggcggcgc tcctgccggc
     5821 gcatccagac cgccgatata gccacgccga ccgccacgat cagcaccatg atggttgcca
     5881 gtgcattgac gtcgggactc acgccgagac gcaccttcga gaagaccacc atcggcagtg
     5941 tcgtggaacc gggaccggcg acgaactgcg atacgaccac gtcgtcccac gagagcgtga
     6001 acgcgagcag ccagccggag agtatcgccg gcacgatcag cggcagcgta atgcggaaga
     6061 acaccttcgc cggtttcgca ccgaggtcga gcgcggcctc ctccagcgaa ttgtcgaagc
     6121 ccgcgaggcg cgactgcacg acgatcgtga cataggccat cgagaatgtg atgtgcgcga
     6181 tcgtgatggt ggtggcgtcg cgcgtggccg gccagccgat gagctgctgc agggcaacga
     6241 acagcagcag cagcgacagg ccggtgatca cctcgggcat gacgagcggc gcggtggtca
     6301 tgcccgagag catcgcgcga ccgcggaacg gcccgaagcg cgcgagcacc aggccggcga
     6361 gcgtgccgag gatcacggcg ccggtcgccg tcatcgccgc aatgcggacc gacagccatg
     6421 ccgcgccgag aatctggtcg ttcctgaaca attcgccgta ccacttgagc gtcggcgaat
     6481 tcggcgcgtc ccacacggtg acgaggcgcg agttgttgaa cgaaaagaag atcatcgaca
     6541 cgatcgggat gtagaggaat gcgaggccga tccacaggcc agtcttcgag aaccagtgcc
     6601 tgccccgcag cctcatttgg aacccgccag ttcacgcgcc tggtagcgct ggaaaaccat
     6661 gatcgggacc atcaggaaca gcagcatcgc gatcgcgacg gcgctcgcca ccggccagtc
     6721 gcggttggcg aagaactcgt cggacagcac ccggccgatc atcagctggt cggagctgcc
     6781 gagcagcgac gggatcacgt attcgccgac cgcagggatg aacaccagca gcgaacccgc
     6841 gacgatgccg ggcaccgaca gcggcagcgt gatgcgcagg aaggacacga tcgggttggc
     6901 accgaggtcg gcggcggcct cgagcagcgt gtggtcgtgc ttctcgaggt tcgaatacag
     6961 cggcaggatc atgaacggca gatacgagta cacgatgccg atgaacaccg caaagtcggt
     7021 ctgcatcatc gtgatcggcg tatcgatgac accggcccaa atcaggaaat tgttgatcac
     7081 gccgttgttc ttcagcagac cgatccaggc gtagacgcgc agcaggaacg aggtccagaa
     7141 cggcaggatg atcagcatca gatagaggtt gcgggtcgcc ggggtcgaac gcgcgatggc
     7201 gtaggccatc ggataggcga gcagcaggca gaataccgtc gagatcgccg ccaccttgag
     7261 cgagtagaga tatgaactga tgtacaggtc gtcggtgagc aggaacaggt agttgccgaa
     7321 attcagcctg atatcgaccg ccttgtcgga gacgaactcc caaagcggtg cgtacggcgg
     7381 cattgcgagc cgcacttccg agaacgagat cttgaaaacg atgatgaacg ggaccaggaa
     7441 gaacagcagc agccacagat agggcaccgc gatcacgagc cgcttgccgg cccagttgtg
     7501 ccgcatgtac tgggtccagc ggggcggacc gaagcggaag aacagatgcc accacggacc
     7561 cagcctcccg gtcagcaggc gggtcgacgg tatcggcggg ggcagtcgcg gcatggtctg
     7621 cggtccgcta ttgcgagagc accacggggc tcgaagcgtg ccagctcagg tacacctgct
     7681 cgtcccacag gatgcgctcg tcgtcggaca gccgctcgat gttgggcatg gtgacccgca
     7741 cgatcttgcc cgactcgatc tgcacgaggt agatcgacag gtcgcccatg taggcgatct
     7801 ccttgacgat acccctgaca cagttctcgc tggcatcggc cggcgcggcg cgcgaaatgt
     7861 tgatcttctc cggccggatc gccgcccaga cgatcgcgcc cggcgccgcg ctgattccat
     7921 ggtcgacata cacgacgccg cccagctcct gcgaacggat gcgcacgcga tcgggcaggt
     7981 cctcgatcag gtggccctca aaggtgttga ccgagccgat gaaatcggcg acgaagcgcg
     8041 tgcccgggaa ctcgtagatc tcgctcggcg tgccgatctg cacgatctcg ccgcggttca
     8101 tcaccccgat gcgcgacgcg agggtcatcg cctcttcctg gtcgtgcgtg accaccacga
     8161 aggtgacgcc gaggcgctcc tggatgttga tcagttcgaa ctgggtctgc tcgcgcagct
     8221 tcttgtcgag cgcgccgagc ggctcatcga gcagcagcag cttcgggcgc ttggcgagcg
     8281 agcgtgcgag tgcgacgcgc tggcgctgtc cgccggagag ctggtgcggc ttgcgctgcg
     8341 cgaactcgcc catcttgacg atctcgagaa tctccccgac ccgctgcgcg atctcggcct
     8401 tcggcagctt ctcctgctcg agtccgaacg ccacgttctt cgccacggtc atgtgcggga
     8461 acagcgcgta ggactggaac atcatgttgg tcggccgctc gtagggcggc gtctcggcca
     8521 tgtccttgcc gtcgatcaga atgcggccgg aactcggctc ctcgaagccg gccagcatgc
     8581 gcagcagcgt cgtcttgccg cagcccgagc cgccgagcag gcagaagatc tcgtgctggt
     8641 agatcttcag ggagacgttg ttgaccgcga caaagtcgcc gaagcgcttc gtgacgttct
     8701 ggagttcgac ataagccctg gcagacggat cgcgccaggg ctcgaccttc ttgtcgttgc
     8761 gtcgttcggc cacgggcagc cttccggggc gggcgctatg cgcccgcccc ttgtgcgatt
     8821 gttattttcc ggtcttgaag cgggtccagc tgcgggtcag ctggcgcgtg aactccgcac
     8881 tctcggccag atcgggcacg atcttggcca tcacttccgg cgtcgggtag atcgacacgt
     8941 cgcccttgac cgaatcatcg atcatcggca gcgcggcggc attgttgctc gcgaagttga
     9001 tgtagttggt gttcttcgcg gcaacctcgg gacgcatcat gtaatcgatg aagatgtagg
     9061 cgttcttgac atgcttcgca tccttcggga tcgcgaagtt gtcgaagaac gcgatcgcac
     9121 cctccttcgg gatgttgtac gcgatcgtaa tgcccttctc ggcctccgcc gcgcggtcgc
     9181 gggactgcag cacatcgccg ctccagccca gcgccaggca gatctcgccg ttggcgaggt
     9241 cgtcgatgta cttcgacgag ttcacgtagc gcacgtacgg ccgtatcttc atcagcacgt
     9301 cttcggcggc ctgcaggtcc tcgggcttct cgctgttggc gtcgcggccg aggtagatca
     9361 gcacggtgcc gatgacttcc gagggcgcgt cgagcagcgt gaccccgcag tccttgaact
     9421 tcgagacgac cgccggatcg tagaacatgc gccagctgtc gaccggtgca tcgggcatcg
     9481 cggcggcgat cttctcgacg ttgtagccaa cgcccgaggt gccccacatg tagttcaccg
     9541 aatgctcgtt gcccggatcg tgcagcgcca cgcgctcggt gatcaccgga tcggcgttga
     9601 cgaggttcgg gatcttcgac ttgtcgagcg gcatgaacac gcccgccttg atctgacgct
     9661 cgaggaacga cgcggacggc acgaccacgt catagccggt gctgcccgcg agcagcttgg
     9721 tctcgagcac ctcgttcgaa tcgaacacgt cgtagttgac cttgatgccg gtttccgcct
     9781 cgaattccgg gatcacggac tcctcgatgt agtccgacca gttgtagacg ttgaggatct
     9841 tttcctcgtc ggtgtcgagc gcgcccggtg ccgccgcatc ggccgccggc gcagcttccc
     9901 ctgacggctt gccgccgcag gcgacgacaa acgccgcggt ggcgcccaac acgagtagac
     9961 gtagtgagat ggacatatgc aaaccccctg tggccggccc gccagttata taacaggcgt
    10021 agccgatatc atagcgccgt tacgaccagc cttcccgggc gatcactgcc gccgtctggt
    10081 cgagcgcgcg agcgaggcgc gccaccagtt catcaatctc ggccggcgtg atcacgagcg
    10141 gcggagcgca gatcatcgtg tcgcgcaccg cccgcgaaac caccccgttc gcgacgcaca
    10201 ggtcgcggca cacggtcccc acctcgcccc ggttcccgaa gaagcgtcgc gacggcttct
    10261 ccggcacgag ttcgagggcg ccgagcagcc cgacggtgcg cacttcgccc accagcgggt
    10321 ggtccgcaag gctgcgcaaa cgggtggcga agtgggcggc caggctgtcg cgcacgcgct
    10381 cgacgacctg ctcgcgacgc atgatgtcga tgttggcgac cgcgaccgcg caggcagccg
    10441 ggtgccccga ataggtaaag ccgtgataga actcgccgcc ctcggcgatc agcacttccg
    10501 cgacgcgatc ggccaccatc aggccgccga tcggcaggta acccgaggac atggccttgg
    10561 cgatcggcat caggtcgggg cgggtcccga aatgctcgca gccgaaccag cggccggtgc
    10621 ggccgaaacc gcagatcacc tcgtccgaca ccagcaggat cccgtagtgg tcgcagatgc
    10681 gctggatctc cggccagtag gtctccggcg gaatgatcac gccgccggcg ccctgcaccg
    10741 gctcgccgat gaacgcggcc acccgccgcg gcccgatctc ctcgatcctg tcggcgagct
    10801 cgcgcgccgt gcgcaggccg aactccgccg gcgagagatc cccgccgcgc tcgaaccagt
    10861 agggctgatt gatgtgcacg atgcccggga tcggcaggcc gccctgctcg tgcatcggct
    10921 gcatgccgcc gagcgacgcg ccggcgacgg tgctgccgtg gtagccgttc cagcggccga
    10981 tgatcacgtt gcgccccggc tcgccgcgca gctgccagta gcggcgcacg agccgcacca
    11041 tcgtatcgtt cgcctcgctg cccgagccgg tgaggaagac gcggttgaat tgcggcggag
    11101 tgacctcggc gagccgctgc gccagcgcga tcgccggcgg cgtcgcgcac tggaagaagc
    11161 tgttgtagta cggcagctcg agcatctgcc ggtaggcggc ctcggccagc tccttgcggc
    11221 catagccgag cgccacgcac cacaggcccg ccatgccgtc gaggatgcgg ttgccgtccg
    11281 aatcggtcag ccacacgccg tcggcgctcg tcaccacgcg ggcgccacgg gcggcgagct
    11341 gcttgtagtc ggtgaagggg tgcaggtagt gggcggaatc cagctgctgc aactcggcgg
    11401 tggatggcga cggcattggc actccgggga ctggcggaac tcggtccttc aaacgttgag
    11461 cagcaggtgt tcccgctccc aggaactgat cacctgcagg aacacctcgt attccgattc
    11521 cttgacgatg gtgaatgcgt tgacgaactt gtcgcccagc acgcggatca gcggcgcgca
    11581 ctcgcgcagc ttgcggatgc cgtcgtcgag cgagcgcggc agctggaacg gcaggtcgtg
    11641 cgcgctgacg gcgatcggct cggagggctc gaggccctcg atcatgccga ggtagccgca
    11701 ggcgagcgac gcggcgatcg cgatgtacgg gttcgcgtcc gcgccggaca ggcggttctc
    11761 gatgcgccgc gatgccgggt cggacatcgg cacgcgcagt cccgcggtgc ggttgtcgta
    11821 gccccactgc acgttgatcg gcgcggtctg gaaacgcgtg atgcgccggt acgagttcac
    11881 gttcggcgcg agcagcgcca tcgcgaacgg caggtacttc tgcaagccgg cgatgtgcga
    11941 gaagaacagc ttgctcgggg tcccgtcctc gttcgaaaag acgttacggc cgctcttgcg
    12001 atcgacgatg ctctggtgga tgtgcatcgc gctgcccggc tccttggcgt gcggcttggc
    12061 catgaacgtc gcgtacatct tgtggcgcag cgccgcctcg cgcgccgtgc gcttgaacag
    12121 gaacgcctgg tccgcgaggt cgagcggatt gccgtgcagc aggttgatct ccatctgcgc
    12181 cgggccgtcc tcgtggatca gcgtgtcgat ctcgatgtcc tggtcctcgc agaactggta
    12241 catgtcgtcg aacatcgggt cgaattcgtt gaccgcggca atgctgtacg actgccggcc
    12301 gggctccgag cggcccgagc ggccgatcgg cggtttcagc tggtagtcgg cgtcggcgtt
    12361 cggctcgacg aggtagaact cgagttcggg ggcgaccacc gcctcccagc cgcgttcggc
    12421 gtacagctcg atgatctggc gcagcacatg gcgcggcgcc atggtgacgc ggcggccgtc
    12481 ggcgtagaaa cagtcgtgga tcacctgtgc ggtcggctcg gcggtccacg gcacgcggcg
    12541 cacggtcttc gggtcggcac gcagcacgat gtcgatctcg gccgggctca tcgccgtttc
    12601 cgggtggatc ggataatcgc cggtcacggt ctgcaggaag atgctctccg gcagacgcat
    12661 gcccccctcc tcagcgaact tggccgccgg catcaccttg ccgcgcgcga tgcccgccat
    12721 gtccggaatg atcgcctcga cctccgagat gccgtggtca cgaaagaact gctgaatctc
    12781 acccgccata aatcaccgtt gcctgccctg ggcgcgcact gcgctcgcgg cgccgaacgc
    12841 cgcgaacagt gcacgcgaga atgaattgcc catcacctgc cactcgggat gccattgcac
    12901 cgcgagcgcg aacgccggcg cgtcctcgac acgaaagccc tcgatcaatc cgtcgggggc
    12961 gcgcgcctcg atcgcgagcc cgggcccgag ccgctgcacg ccctgggagt gcagcgaatt
    13021 gacccggatc cggtcgctgt ccgtgagcga ccgcagtacg ccaccggcaa tgagctcgac
    13081 gtcgtgcgcc ggtgcgtact gcacctcgag cggctgcgtc ttgtcctcgc gatggtccat
    13141 gtatccctgc tgctcctgca cccgctgcca gagcgtaccg ccgtacgcga cgttcatttc
    13201 ctggaaaccg cggcacagtc cgagaaccgg gatgccctgc ccgaccgcca gcggtatcag
    13261 cggcagcgtg gtcgcatcgc gcgccgcgtc gtgttcggtc ccgggcacac tcgccggtcc
    13321 cgagtaatga tgcggctcca cgttcgacgg cgagccggtg aacaacaagc cgtcgatgct
    13381 cgcgaacaac tcgccggcgt cgatctctgc accgagcgcc ggcaccagca acggcagtgc
    13441 gtcggcggca tcgacgacgg cgcggatata tttctcgccg accgcgtgga acggatgcgc
    13501 cccgagcatg cgccgatctg cggggatgcc gatcactgga cgccgcttcg ccatcggact
    13561 acctggtcat cgattgttta gaatattgaa cacgcggcga ttcccggccc cgtgcctgtc
    13621 cattagacca caccttccgg gaggccggat ggtgcaaagc actatcgggg tattagcccc
    13681 tgcccggccg ccacctgatt tgccgcgcac gcgcactggc gcataagctt gcgacccggc
    13741 tgcccatgga gggtgtccga aatcctaaac gaggtatcga tgtcccgcga aacgcgcccg
    13801 gaactgcagc gttttctcga agcgtacccg gacacgcggc acttcgatgt gttcctgcac
    13861 gacctcaaca ccgtcgagcg cggcaagcgc ctcgacctcg ccggcatgga agccgcctat
    13921 cgcggcggca tgctgctgcc cggttcgatg ttcgccctgg acgtgctcgg tcatacggtg
    13981 gaagccacgg gcctcggctt cgacgatggc gacgcggacc ggccgtgccg gccgatcccg
    14041 ggcacgctgg tcccggtgcc gtggcagggc gcgcacgccg cccaggtgca ggtgacgatg
    14101 cacgaaccgg acggccgcgt cttctacggc gatccgcgcc acgtgctcac cggcgtactg
    14161 cagcgtatcg aggcgctcgg cctcacaccg gtcgtcgcga tcgagtacga gttctacttc
    14221 atcgacctcg agcgcgtgga cggcggactg ccgcagccac cgcgcggtcc gctgaccgga
    14281 cgacgcgagt tccgcaccca gatcaacgcg atggccgatc tcaacgaata ctcatcggtg
    14341 ctcgcgcaga tcacgcgcga ctgccatgcg cagggcgtac cggcgacgag cgcgctgccg
    14401 gaatacggcc ccggccagta cgaggtgaac ctgcagcacc tgcccgatgc cctcaccgcc
    14461 tgcgaccagg cgctgcgctt caagcgcgtc gtcaagagcg tcgcgcgcgc caacggactc
    14521 gacgccacct tcctgccgaa accctatcgc gacatggccg gcagcggcct gcacatccat
    14581 ctgagcctgc tcgacgggga gggccgcaac atcttcgcgt gcgacgagcc ggccacgcat
    14641 gcgccgctgc gccacgcggt cgcgggcctg ctcgactcga tggcggaagg catggcgatc
    14701 tgcgccccgg ggccgaactc ctaccggcgc ttcaggagcg aggcctacgt gccgctcacg
    14761 acgagctggt cgatcaacaa ccgcggctcc gccgtgcgca tcccggtcag cgatgccgcc
    14821 aatcgccgca tcgaataccg ccaggccggc gcggacgcga acccctacct cgtgctcgcg
    14881 tggatgctgg cgggaatcca ccacggactc ggcgcgcgca tcgagccccc cgagccgctg
    14941 cgcggcaatg cgtacgcgca gcccgccggg cagcccctgc cgacgcactg ggaaacggcc
    15001 atcgagcgct ttgcgggcag tgacttcgcg cgcgagtacc tgggcgagcg cttccagcgc
    15061 ctgttcgcca ccgtcaagcg cgccgaactc gcggacttcg gttcatacgt cacgccgctc
    15121 gagtgcgcgt ggtacatggg ccccctgtaa ttccccatga gcacgagcga gtacgtcgac
    15181 agctattacg ccgcctcgcg acacccggcg ccatcgcatg cgccgctcgc gggcgacgtc
    15241 gactgcgacg tgtgcgtggt cggcggcggc atcgcaggct gctcggccgc gctgcacctc
    15301 gccgagcggg gctatcgcgt ggtgctcctc gaagcggcgc ggatcggctg gggcgcctcg
    15361 gggcgcagcg gcgcacaggc cctgttcggc ctcgccgcgg gcgaggagaa aaccgaagcg
    15421 ctggtcggcc gcgaggacgc ccgccgcatc tgggacatga cggtcgaggg cctcgccctg
    15481 atgcgcggcc tgatcgcacg ccacgcgatc gactgcgatt acgtacccgg gcagatgcac
    15541 gtcgcagtgc gaccgcgaca ggtcggcgaa ctgcgcaact gggtcgacct gatccacgcg
    15601 cgatacgact accgcagcct gcgcttcgtg ccgcgcgagg aactgcgcga tctcatcgcg
    15661 agcgagcgct acctcgcggg aaccttcgat tccaacagcg cccacctgca cccgctcaac
    15721 tacacgctcg gcattgccgc cgcggcgggc cgcgccggcg tgcggatcca cgaaggcagc
    15781 cgcgcgctcg actacgaggc gggtgaggtc gtgaccgtgc gcaccgcgca gggacgcgtg
    15841 cgttgccgcc acctcgcgct gtgcggcaac gcctggctcg gcgacacggc acccgcgatc
    15901 gcgcggcgca tcatgccggt cggcacctac atcgtcgcga ccgagccgct cggcctcgaa
    15961 cgcgcccgcg ccctgatcgc caacgacgcc gcggtgaccg acatcaactg gatactcgac
    16021 tacttccgcc tgtcgcgcga ccaccgcctg ctgttcggcg gtcgcgtgag ctactcgggg
    16081 ctcgacccgt tcaataccgc gcaggcgacg cgccagcgca tgctgcgcgt gtttccgcag
    16141 ctggccgacg cgcgcatcgc gtacagctgg ggtggctacg tcgacatcac gcttaaccgc
    16201 gcacccgact tcggccggct cgcgccgaac gtgtactacc tgcagggctt ctccgggcac
    16261 ggcatcgtgc tgaccggcat cgcgggcaag ctgctcgccg aggcggtggc cggcacggcc
    16321 gagcgcttcg acgtgtttgc gcgcatcagg caccgcgaat tcccgggcgg cgcattgctg
    16381 cgccggccag cgctcgtgct cgcgatggcg tggtatcggc tgcgcgacct gctctgaacg
    16441 ctcagcgcgc gcgaatctcg aaacaggtgt ggatgcgggg attgcgggcg aagtccgcag
    16501 ggatagtcgc gcgcgtgaca tcggtgagcg cgtagcgctc cgcgagccct tgatcgatgc
    16561 ggaatttctg cgcattggtc gagaacacga gcagcccgcc gggcgccagc agccgcatgc
    16621 aggcgtcgat cagtgccgga tggtcgcgct gcacgtcgtg cacgccctgc atgcgcttcg
    16681 aattcgagaa ggtcggcggg tcgaggaaga tcaggtcgaa gcgctcgccg aggcgcgcgg
    16741 cctcgccgag ccattcgcgg caatccgcct ggacgaaccg gtgctggcgg cctgcgaggc
    16801 cgttcagggc gaggttctgc tgcgcccact cgagataagt gcgcgagaga tcgacgctcg
    16861 tcgtcgcgcg tgcgccgccg gcggcggcgt acacggtcgc gctgcccgtg tacgcgaaca
    16921 ggttgaggaa gcgcccgccg gccgcagctt cgcgcagccg cgcgcgcgtc aggcgaaggt
    16981 cgaggaacag cccggtgtcg aggtagtcgg tgaaattgac acggaagcgc agcccggact
    17041 cggcgacgac gtggaactcg ccacgctccg cttccttttc gtattgctcg ccccgtccgg
    17101 tacgccgccg catgcgcacc atgacctggc tctccggcag tcccagcact tccggcagca
    17161 cggacaaggc ctcgccacgc cgccgccgca ccgcctcgag ctcgatctcg cgcggcgccg
    17221 cgtactcctg cacataggcc cagcgcatcg gcgagtcggc agcggtgtag aggtcgatcg
    17281 cgaacgcata ttccggcatg tccgcgtcgt agacgcgata gcagccgatg ccggcgcgcg
    17341 tggcccagtc tccgaggcgt ttggcgttct tgccaagccg gttcgcgaac atgcgcgcgc
    17401 cgggcgtgtc ccgcagcgcg gggtcggcga ggcgcgcggg ccggggactc gtgaaatcgc
    17461 gcacgctctg cggcgacacc tcgatgcgca gcagacggca ctcgagcgca ccgttccaga
    17521 ccgtgtgcgt acgcgccgcg cgcaggccga tctcgaggcc catctccggc gcgccggtga
    17581 tcaccgccgc gcgccagccg ttgaaatgct cgcgcagcac cgcgccgagt tcgcgatgca
    17641 gtgcgcgcgc cgtttcgcga tcctcgagcc gcacaccgta gggcgggttc acgcacagca
    17701 ggccgcgcgc ggcgttgggt cgcgcgtccg cgaggcgcgc ccgcgtgaac tgcacccagt
    17761 ccgccacgcc ggcgcgcgcg gcattctccg cggcgagacg caccatgcgc tcatcgatgt
    17821 cgctgccgcg caggcgcggg acaccaccgt catgcggcct gacgcgggac tgtgcttcgg
    17881 cacgcaggcg ctgccacagc gcggcgtcgt ggcccttcca gccggtgaaa ccgaagtact
    17941 cgcgcgcgag acccggcgca atgtcggcgg cgatcatcgc gccctcgatc acgaaagtac
    18001 ccgagccgca catcgggtcg agcagctcgg cgccctcgct gacgagctgc cgccagcccg
    18061 cacggaccag gatgcccgcg gcgacattct cgcgcagcgg cgcctcgccg gcctgtgacc
    18121 ggtaaccgcg acgatgcagc ccctcgccgg acaggtcgat cgacaccgtg accttgctgc
    18181 cgtgggcatg cgcatgcacg cgcaccgacg ggcgttcgag cgacacgtcc ggccggcgcc
    18241 cgtatcgctc gcgcagcgca tcacagatcg cgtccttgac gcgttgagcg ccatagcgcg
    18301 tgtgattgat gcccggatgc tggcccgagt agtcgcacgc gagcgtgccg tccgggtcga
    18361 catgctcgcc ccagggcagc gcgtgcagcg ccgcgtgcag ctcatcggca ctcgccgcct
    18421 cgacctgcgc cacctcgagg aacacgcggc ttgcgaggcg cgattcaagg caggcgcgat
    18481 aggcgacttc gagcgttccg gagaacgaca cgccgagcag gcgctcgcgc acgtccgtgc
    18541 cacccagcag ggtgatctcg gcggcgagca ggtcgccgaa gccgcgcggg gcgcttgcga
    18601 gaaagcgcat cacgcccgcg gcggccaggg cacgacacct cgcggccgcg ccgccaggaa
    18661 tcccgtgagc gcgcgataga cgtccacatc gaatccgacc gctggcccct gcacggccag
    18721 ctccggctcg ggcggcaggt cctccacgcc ctcgtggcgc gtcaccgtgc cgagatagcg
    18781 ccagccgtcg atgacgtgcc actgctcgaa gccgccgaat gcgcgctcgt ggaccgccac
    18841 cgcaccctcg tagggccagg gcttcaggcg ctgcggcgcg aacgcgagct tgctgcgcgc
    18901 gagatggcgc tgcgggctct cggcgccgac gcaggcgcca cggcagcgct gcagctggta
    18961 ggcgaaacac gagccctcgc cgccctcgag gccgagcacg cgcgcgcaca gcccatgctc
    19021 gcgcgcgaga tgttcgagcg cgctcgtcgc ctcgcgcgtg gtgcgataga taccgaagca
    19081 gtcgccagcc ggccagccgt cgtccagcga cacgagccgc ggcgttgccg cgtcgtcttc
    19141 gaactgccag ctgagcgcct cgccgccgcc gcgcagtcga cggttgtaga ccgggagcga
    19201 ctcgcgcacg cagcgcgcct cgagcagcat tgcgccgagc tcgccggcgg tctcggtcca
    19261 ctcgacgcgc gtcgtctggg tcgacaggcg gcgcgccttc aggctgcgcg gcgcgccgcc
    19321 ccagtgcgcg agtacgcgct cacggaggtt cttcgctttg ccgacataga gaggggcgtc
    19381 gccctcgccg taaaaacgat agacgcccgc tccctgcggc agttcgtcgg cgagttccgg
    19441 cggcaactgc ggcgggatcg gtgtccggcg cgtgacttcg gccagcgcgg cctcgagccg
    19501 cgcgggcggc cagctcgccc gcagcacccg ccagaactgc cagagcgcca tcgcatccgg
    19561 cagcgcgcga tggcgcgccg cgcatgccag ctcgtgatgg gcgatcagcg cgtcgagatt
    19621 gtggtgcgcc atctcaggat agaaacgccg cgagagcttt actgtgcatg cgaggcgcgc
    19681 gtcgaacgcg tgcccggcgc gcttcagttc gttgcgcacg aaaccgtagt cgaaccgcgc
    19741 gttgtgcgcg atgaacaggc gcccctcgag gcgcgtggcg agttcgtccg cgatcgcccg
    19801 gaatggcggc gcgtcggcca gcgagccggg gtcgatgccg gtcaggcgct ggatcgtcgg
    19861 cggcagccag gtgccgggat tgaccagtgt cgtccacgac tcctcgatct tctcgccctg
    19921 tgcgagcacc acgccgattt cgatgatgcg gtggtacagc ggatgcccgc cggtcgtctc
    19981 gacatcgacg aacgcgagcg gcgcatcgag cagcgcggtc acgacagatc gagccaggtc
    20041 gtcttgagct cggtgtactt gtcgaacgca tgcagcgact tgtcgcggcc gaaacccgac
    20101 tgcttgaagc cgccgaacgg caccgtgatg tcgtcggcgt cgtagcagtt cacgtacacg
    20161 acgcccgcac gcagggcgcg ggcgacgcgg tgcgccgtgc gcacgtcgcg ggtccagacc
    20221 gcggcggcga gaccgtagtc gacatcattc gcgatctcga cggcctcctc gagcgtgtcg
    20281 aaggtgatcg tcgccagcac gggaccgaag atctcctcgc gcgcgatgcg catctcgcgc
    20341 ctgaccccgt cgaacaccgt gggctcgacg aagtagccgc cactgtcctc gcgcacgcgc
    20401 cgcccgccga aggcgacttt cgcgccctcc ttgcgcccgg tatctatgta gccgaggacg
    20461 cgcttcatct gcacctcgtc gacgatcgcg ccaagcgtgg tcgccggatc gagcgggtct
    20521 ccaggctgca tggtgcggcc gatcgcctgc acgcgctcga gcaccgcgtc gcgcaccgtg
    20581 cgctgcacga gcaggcgcga accggccgag cacatctcgc cctggttgaa gaagatcgca
    20641 taggccgcgg cggtcgccgc gcgctcgatg tcgggatagt cggcgagcac gatgttcggc
    20701 gacttgccgc cgcattcgag cgatacgcgc ttcatgttcg actgcgcggc gtactgcatc
    20761 accagcttgc cggtggcggt cgagccggtg aaggtgatcg catcgacgtc catcgacagc
    20821 gcgagggcct tgccggccgt atgaccaaag cccggcacga cattgaacac cccgggcggc
    20881 agtcccgcct cgattccgag ctgcgcgatg cggatggcgc tgagcgggga tttctcggaa
    20941 ggtttgagca ccagcgaatt gcccgcggcg agcaccggac cgatcttcca ggcggccatg
    21001 gccagcggga aattccaggg cacgatcgca ccgacgacac ccatcggctc gcgcgtgatc
    21061 agtgccagcg agtccggacc ggtgggcgcc acctcgtcgt agatcttgtc gatcgcctcg
    21121 gcataccact cgatgcagcg cgcggcaccc gggatatcga tcttcaggct gtcgctgatc
    21181 ggcttgccca tgtcgagcgt ctcgagcagc gccagctcct cgcgatgacc ctggatcagc
    21241 tgcgcgaact tcgccagcac cttcttccga tgcgagggcc tggtctgcga ccagacccca
    21301 ctgcgaaacg ccgcccgggc cgaggcgacc gcgcgctcga tatcggccgt gtcgcccgcg
    21361 gcgacgcgac cgaacacctt gccggtcgcc ggatcgacgt tgtcgaactg ctcgcccgag
    21421 agcgccccga cgtagtcgcc gtcgatgaac gctcggccct cggcggtgag ttgcgcggct
    21481 cgcgcgtgcc aatcgatcac gggctttgct gcggacatgg tgacctcggt cagaaagtgg
    21541 acggcgcgct cgcgctgatg atctcacagg gctcgcggcc gacgttgcgg aagcggtgcg
    21601 ggatcgaact ggtgaaatag taagcctcgc cgggcccgag cacgcgcgtg ctgcccccga
    21661 ccgtcagttc gatccgcccg cggatcacga tgccgccctc ctcgccccgg tgggcgagcg
    21721 catcgtcgcc ggtcgcggca ccggcggcat agcgctcgtg cagtatcgtc acctggcgcg
    21781 ccgggcgctg cgcggcgacg agtcgcagcg acacatcttc ggtgccgatc tcgatgagtt
    21841 cgtcgtcgcc gtagaaaatc tgcgccgggg cgtcgacatt catcgtgaag aattccgcga
    21901 gcgacatcgg cacgccgtcc agcactttct tcagggaacc gaccgagggg ctcacgcggt
    21961 tctgttcgat cagcgagatc agcccgttcg tgacccctgc acgccgggcg agctcgcgct
    22021 gcgacagtcc gtagatcagg cgcacgctgc gcagccgcgc cccgacatcg atgctcatgt
    22081 tccctgcctg tttagcatca tggacgaaat acgctaaatc tcggcccagt tcgccatgca
    22141 tgtcaacttt ccttatcatt ccggcatgag cacctcgcac accagcctcg atgccctgtg
    22201 gttgccgttc acggccaatc gccagttcaa gcaggcgcca cgcctgctcg cgcgcgcgag
    22261 cggcatggag tacttcaccc ccgaggggcg gcgcgtcctc gacggcgccg ccggcctgtg
    22321 gtgcgtgaac gccggtcacg ggcgcaagga aatcaccgca gcggtcgcgc agcagctcga
    22381 aacgctcgac tacgccccga cgttccagat ggggcatgag cgcgcgttcg aacttgcgac
    22441 ccggctggcc gagctcaccc cggccggcct cgatcgcatc ttcttcacca attcggggtc
    22501 cgagtcggtc gacaccgcgc tcaagatcgc gctcgcctac catcgcgtgc gcggcgacgc
    22561 ggcgcgcacc cggctgatcg gccgcgagcg cggctaccac ggcgtcggct tcggcggcat
    22621 cacggtcggg ggcatcgtcg cgaaccgcaa ggcctggtcc gcaagcctga tcccgggtgc
    22681 cgatcacctg ccgcatacgc acgatctcgc gcgcaacgca ttctcgcgtg gcctgccgga
    22741 gcatggcgcg gaactggccg acgaactcga gcgtctggtg gcgctgcacg acgcgtcgac
    22801 catcgccgcg gtgatcgtcg agcccgtcgc gggctcgacc ggcgtgctgc tgccgccgaa
    22861 gggctatctc gagcggctgc gtgccatctg tgaccgccac ggcatcctgc tcatcttcga
    22921 tgaggtcatc acgggcttcg gccgcaccgg ttcaccgttc gccgcgcagg aattcggcgt
    22981 aacgccggac atcatcacga tggccaaggg cctgacgaac ggctgcatac cgatgggtgc
    23041 ggtggccgtg aagcagtcga tccacgacgc gttcatgcag ggaccgcccg gcacgatcga
    23101 gctgttccat ggctatacct actcggcgca tccggcggcc tgcgcggcgg gccttgcaac
    23161 cctcgacatc tatcagcgcg agggactgct gacgcgcgcg aagacgctct cgaagac
//
LOCUS       DAOQVY010000030        22527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_2694_length_22527_cov_7.876735, whole genome shotgun sequence.
ACCESSION   DAOQVY010000030 DAOQVY010000000
VERSION     DAOQVY010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 22527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22527
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2694_length_22527_cov_7.876735"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..628
                     /locus_tag="PKN91_04535"
     CDS             59..628
                     /locus_tag="PKN91_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27373.1"
                     /translation="MSDTNKIPARWYCLGSTGTAMLCKDEEDARNKAAFADNARPHHA
                     PYHAVRLGPVDELTEAMTRMSNALLGDAADEIERLRAELAAVRRYQAGNPLGGPASMF
                     ATAASRIEAGESLDDVLHDYGWCQEAERDRLREGARYMARVWILSDDDPQSLEAVDNC
                     VDGCCVAAETRNREIAALDGAAGGGHDRR"
     gene            615..824
                     /locus_tag="PKN91_04540"
     CDS             615..824
                     /locus_tag="PKN91_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27374.1"
                     /translation="MTAADPLVQRLRAAAALPTGLYAEAAARIESDAAKIAALVEQHH
                     RDSAELRALCEARDEARRERDRLTA"
     gene            complement(1026..1904)
                     /locus_tag="PKN91_04545"
     CDS             complement(1026..1904)
                     /locus_tag="PKN91_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27375.1"
                     /translation="MLISYSRPESIAWTLVGTGAAFLTDDDRLTNGRPESGTRIQWLS
                     GAQTTASVLDMQAVWPAAATIRLCGLVGLTLPVGTRIVCGLRRPADANFDYVTAECRV
                     VQRQDGVRVAWFPFDAGLEPCIGAEFAIYNDVGGSSPIVADSTFSIGEAWIGEGEAWC
                     IRPQYESSRSDLSRLNKSIGGQPFPVRRRAEALSQLEFTPQVYTDAYARTGSLADLRE
                     RMLAYEPVVVVPITGEPFTRTPITLANVNRHAEFGYCSSPGPIVGDAPRFVMSAQFIA
                     PPALLPIEAVTDSSGS"
     gene            complement(1906..>3846)
                     /locus_tag="PKN91_04550"
     CDS             complement(1906..>3846)
                     /locus_tag="PKN91_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27376.1"
                     /translation="TGQRAFEFPVAGAEGWFEYPPEVPTIRVSTRDLILPPDDPIPNA
                     VYEGRLRDDNIEIVQSLAFWPDGRSQTQGSAVNITIANGDGALDGLLDFDLRDLPVTL
                     EWIEQGQSILSADHVADMIVDDLQVLDDGALRLRLSSAMSVLDAPLQNMIFPPSADPV
                     VAGKPWPILEGAARSVTPVLVDPVNRIYALSDRAIVGWGYIRDMGAPLDPNDIPPGYV
                     ISDDFRGVVLETEPVGKLTADASSTGGGTLPSLADDIWLGYGRPFAEDSNGDLIGFDD
                     VVDAAYLAIGRLLFTSSSLAGVGRVALTSAQMDEGRHYRYRVVVQYIAPPHSFGQPYV
                     ALTTAGGIPLVSWTTSGTYEGVITASANIVPRLVFGTALHGTQAIIREAYILEIPDTY
                     TPSNINAITLTDFVRSIVEDRAGLPASAWSRDDTEAIDAATGYAGIGFYASEPTTVRA
                     ALESVLNSYCACAWVDSDGVLRFTRLTPPEDEKAVGEITAAEMLSEPIVRPDLAPNLS
                     TQMRFRKNWTVLGEGDFVTDFVVVPLAVRQALSQRFQGIAASATPLSATYAQAVNADP
                     AEVLLDVAEDAQAEIDHVAGYYGTLRKIYTFEVSAELPVQIGQVWTLVYPRHGLGAGR
                     NLLVSGMSRRLGAETLLLTLRG"
     assembly_gap    3847..3946
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3947..4505)
                     /locus_tag="PKN91_04555"
     CDS             complement(<3947..4505)
                     /locus_tag="PKN91_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPRY domain-containing protein"
                     /protein_id="HNS27377.1"
                     /translation="MSGLRYSTFDPAALGPLLALESANTIVLPTATTDINRTARALYG
                     KQAFSWYAEFAAWGDDDISDAVSFGLVTEDASLSTYVGGDADGYGYRVAEGQIHNAGA
                     SVQAVTAGGPGDIVGVWLNIIDTTATVTWFLNGIALHTVTLPSTGPWYLAASVSAPRS
                     DIIKAWLNTGQRAFEFPVAGAEGWFE"
     gene            complement(5519..6184)
                     /locus_tag="PKN91_04560"
     CDS             complement(5519..6184)
                     /locus_tag="PKN91_04560"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HNS27378.1"
                     /translation="MKYQRILALTINPGLAIVGILPPVGYGVSYSATLVGYGGIEPYQ
                     WALVSGFLPAGLSFGVDSNGDFVLSGTTSAVGLYPITVILRDSSGKSVTRQFKLRVVA
                     EPLVIAGSAPDGDVGVPYSYTYTVSGGVQPYMFGLISSYLPDGLYITSGPGADEVTIE
                     GTPTVSAVNHSFTIGVWDAQTPTAAYAQITDTISIVSVSYHIMTEVGDAIAAESGDLL
                     RTE"
     gene            complement(6181..10593)
                     /locus_tag="PKN91_04565"
     CDS             complement(6181..10593)
                     /locus_tag="PKN91_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27379.1"
                     /translation="MIISIALRALTGTFITDMGRAEKETARAAKKMQKDLEEAGKVIG
                     TALAAVTTATAALVKSAIDNMDRLDEAAQRVGVSAKQLSTLEYAAKRGAVSSEQLEAA
                     LGKLAKSASDTAAGTGTAAKGFEHLGISVKNTDGTLKSTEQLLKEAATEFAKYEDSAQ
                     KTAVAMAIFGKSGKDMLPFLNQGADGMRELQDRAKALGLEISDAAAAQAGDFNDAMGD
                     MADLVRGVGNDIAQALLPKLTQIAEAFVDAGVGAREGGNNFEWLGTVAEYVAKTFTVL
                     KAVIEGLTNLVAATFDSIVGVGQVAAGAVGGVVEGIKGSLKQLTGDFAGANESFMKMR
                     GNFAEGWTEGAKTISTAWSTATDGIEVALAGMNATLDAIDKPIEKAAGSTETLRKNMQ
                     AFADEGAMAAAAKAADGLESAIRKIVDITSQLTAVDPVDQAWADYEKTMRDIADAAAK
                     AEAENAKLGARGIEQAEILRMVDDAENAAADARERTIAAIERERDVTGQYVAELADEA
                     RLIGLTTAQQRAETIVMRALADAKRLNAAAGKEVVKVDTERLRALAKINEALSKAATI
                     NDASKSPILEMVDQAKELGDAIKAAFEEGMDPEIIAPMQKAVEKLNAQVAIQTLGSYK
                     ALLGAMQTFTKEGSKGFKAIEKGMAALQIVQDIIALKAAVTAVLTQGQGEPYSAWARM
                     AAMAAAVAPFIASIGLSLKSFGGGGGKDMTSENRQASQGTGSILGDATAKSESILNAV
                     EITANATQQLVGINRGMLNALHSLQNALGAAGNQLARGAADVEFGGLSQGFNLDILGK
                     DPLTRSLSNAIFGGGRKIVDNGIIIAGGALNDMLESIVVGAYQTIRTDGGLFGRDKIK
                     DRIIDISDTFGRQFQLVIGSIADTVREGALALGLLPEEVEAALAAFRVEEIRISLKGL
                     DAEEQQKEIEAVFSRLFDGLAGAVVPWIDQFQQVGEGLGETLVRVATGVQVTQEAVRY
                     LGFALDETDPEKFAQISEGLLELVGGIDAMIEGMTAFMGAFAPANHLLEVAAGSLTSA
                     FEQAGLVLPETRDAMWALMQTFDATTEEGREQIATLIRLAGVARDYFDLLDKAEKARL
                     DYAIKASELMVELGQAGGFIAGRAEIEKWTTDTIRSLNDLARAAGRAGASEQDLVNVH
                     AVAAQRIAALIAKLKDESRDLAATLGYITGGSGDTIESLNAQIASLSGASGDAADAIG
                     QAIDGMREKMNLLLGDLSPFNDQRKLELALQGLREGTVDPQQVLEIGRRLYASTSNYT
                     NLFNQVMGMANFRSTTPGGNVGSSGDGRSLDELIAARDALVAAQRPEIADQLARRIAE
                     LSYATGDSFAELAESMGWTLGQLGADLSLTDEKLQAYLESLVAQFEAQDWSAISESFD
                     DSITTNTDRIVDAIEGRTASLSEQMESTTIVAEKLESIAVRIDSLASTIVSSDRQTDA
                     TLGGALSGMAQETRELNLEIRASGGRRVAWVLER"
     gene            complement(10590..10844)
                     /locus_tag="PKN91_04570"
     CDS             complement(10590..10844)
                     /locus_tag="PKN91_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27380.1"
                     /translation="MRLIATYLSREPAPDERIELALAQIATMYANSHRPKDRPPAKLV
                     DFLPARDAWKAAPAITPSSDRYSESDRLMLSALMNRSPPL"
     gene            complement(10934..11290)
                     /locus_tag="PKN91_04575"
     CDS             complement(10934..11290)
                     /locus_tag="PKN91_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27381.1"
                     /translation="MELIKKTLKHKGTETTVYFRELTAGEQLSLVKGQTYRGNAKSGN
                     VEIDVGENVESQQRLVLMTLVDENGNQVYAHIKQLHAEPASKIIALGKLAEEAQRADV
                     DDAGDALPAGEAEGNG"
     gene            complement(11403..11921)
                     /locus_tag="PKN91_04580"
     CDS             complement(11403..11921)
                     /locus_tag="PKN91_04580"
                     /inference="COORDINATES: protein motif:HMM:NF027775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HNS27382.1"
                     /translation="MTEGVIETKYTRLCFGYSPGASSSDPDGTIVLQVACPTGIPDLT
                     SGARPKQDMTCLSSPVREYFSGLADPGELAIPINFIPRSESHQALIAAKEQGANLVMP
                     WMVVFSDQAALPVSVDSDGNLLSPGSTTVGFRGYVSNFAINAQVGDIWRGTVTIQVNS
                     ELAWDLPAADLP"
     gene            complement(12036..12464)
                     /locus_tag="PKN91_04585"
     CDS             complement(12036..12464)
                     /locus_tag="PKN91_04585"
                     /inference="COORDINATES: protein motif:HMM:NF022810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HNS27383.1"
                     /translation="MNLPSLFPLIAADPSVTAFIGTSPVRFYPHGTAPQGVVAPYLTQ
                     FASTILPINTLDRGAARADSTLVQVSCWSDNGGDAVDELRDLAAAARRCIERAHHVEA
                     VRDMRKDIETGRYRIDFDVRVFVHREDDASSSDSISSSST"
     gene            complement(12461..12979)
                     /locus_tag="PKN91_04590"
     CDS             complement(12461..12979)
                     /locus_tag="PKN91_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HNS27384.1"
                     /translation="MAVETVSVQGLAGVLDALRKLPPEIVSKAGGPVKLALKKAAEVL
                     RDEAKRNVQRIIDTPNKSGENESTGLLMKSIQAKRSRMRGGERGEAFVVGIKRGQRYP
                     QGRQGRKGGITAVQVGRLLEYGTERRQPMPWLRPAFDAKKGAAVQTFVAEMRARTQKV
                     IDRVEREARAKG"
     gene            complement(12969..13328)
                     /locus_tag="PKN91_04595"
     CDS             complement(12969..13328)
                     /locus_tag="PKN91_04595"
                     /inference="COORDINATES: protein motif:HMM:NF017343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HNS27385.1"
                     /translation="MDAQRYRHRVNIQEKVAGPQNPRTGAVPYTWETVWLDSDTPLNE
                     VPAEVLTGRGREFYAADAKQAETTARIQMPWFPGLLPTWRILWDGKVFDITGIETDAT
                     ARRQYRITCKDGLTDGS"
     gene            complement(13329..13664)
                     /locus_tag="PKN91_04600"
     CDS             complement(13329..13664)
                     /locus_tag="PKN91_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HNS27386.1"
                     /translation="MSFVDLATVKAWLRVFQSADDPLLQQMIDQAEDEALRFLNRTEP
                     PTLPLDYPSECESEQVPSSDDPIAPSFEKAVCILVQAAYEQTDPDKAERMRRNAETVL
                     MPYRRGLGV"
     gene            complement(13651..14337)
                     /locus_tag="PKN91_04605"
     CDS             complement(13651..14337)
                     /locus_tag="PKN91_04605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNS27387.1"
                     /translation="MLQVLTTTGCRPEAFALCARWMARQTFRGQVRWIVVDDGEEPTP
                     MPAELPENWCIDVVRPMPFWREGDRTQPRNFVAGLDAALQAPVVVVEDDDWYAADYLE
                     TCAARLREHDLVGVGPQRWANVRLQRVGAYRAAWPWTSSMAFNGRVIEHLRAIAASRE
                     AYVDRDGWELYAGRKRYFPDGRVVGIKGLPGRGGYMPCHHQPQGSRDPGLRMLERWIG
                     SDVEAYREFR"
     gene            complement(14322..15020)
                     /locus_tag="PKN91_04610"
     CDS             complement(14322..15020)
                     /locus_tag="PKN91_04610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNS27388.1"
                     /translation="MTLQVLTTTGGRPEAFGMCVEYMRRQTYTEPVRWVIVDDGEPGI
                     DAPTMPATWKVEVMRTRPVWQPGQNTQARNLRAGLLAIDGEVPLVIVEDDDWYSPAWL
                     QTCADALTHCELFGEAGARYYHVRRRVARNMGNVAHASLCSTATIGRATRMLFSLAGR
                     HASGLDMRLWRECRVPAMLTVATHVVGIKGMPGRGGIGIGHRDDFAGDQDDDLSVLRS
                     WIGDDAERYACFRS"
     gene            complement(15017..15577)
                     /locus_tag="PKN91_04615"
     CDS             complement(15017..15577)
                     /locus_tag="PKN91_04615"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNS27389.1"
                     /translation="MNRLPPNSVRGQVRAWIEKHAAGLGDDVLEVGSRMHTPDAWWVV
                     NRDLARGQWTGIDMQPGPNVDVVADLHDPPDEWIGRFSGVVLSEVLEHVRNPWTALVR
                     LRALMQPGALLIVTVPFCFPRHAFPDDFYRFTESGLRAVLEVSGYKDVATANSGPNVA
                     FALNDHGERGLIRRQEPLHTFAVARA"
     gene            complement(15555..16004)
                     /locus_tag="PKN91_04620"
     CDS             complement(15555..16004)
                     /locus_tag="PKN91_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27390.1"
                     /translation="MFKAAPWADALYAIDPPWWKVYGQDVIESFRGLRFSGRQQHGFA
                     QASPFQTFSNSGAAAIALSAWMGARRIVLAGYDCQRTGGKSHSHGDHPPSLGNAKSMP
                     QWFERFAQCSRQMRALGVEVINASRATALTCFPRGTLEEQLEQAATE"
     gene            complement(16019..16327)
                     /locus_tag="PKN91_04625"
     CDS             complement(16019..16327)
                     /locus_tag="PKN91_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27391.1"
                     /translation="MIVTALSTFNHCGVTYKRGDTPNITSTAARALREKGLVSFSGLP
                     ADEHPPQAGGTDSPSSASLPAQAAPQTTSSESDAGDSEPAKGKRKKAKDDPEEPAGEP
                     "
     gene            complement(16394..17587)
                     /locus_tag="PKN91_04630"
     CDS             complement(16394..17587)
                     /locus_tag="PKN91_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HNS27392.1"
                     /translation="MAADGDIKAINESLKQVGDQLKAHAEKADKEIKAHAKLSEETRA
                     KVDELLMQQGELRANLQSAEQKLAKLEQGGGEPERPQTMGEQVTANEGFQTWAKRPAG
                     SFAMGVQAKITEGAASAGDLILPDRIPGIQQPGLRRLTISDLIAWGRTTSNSVEFARE
                     LVFTNSAAPVSENPSAGKAESNITFEAAQAPIVTIAHWIHASKQVLADVPQLQSYIDG
                     RLRYGLKLVEENQLLNGSGVGLNLDGIYTQATAYSNPGVTVQAETRLDRLRLALLQVE
                     LAEYWADGIVISPIDWAAIELTKTEDNAYLFANPRSVNLPGLWGRNVVPTQAISADDF
                     LVGAFGGGLAVQGWDREDVNVVISLEDRDNFIKNMATIRCEERIGLTVYRPAAFVKGG
                     FDDLS"
     gene            complement(17599..18456)
                     /locus_tag="PKN91_04635"
     CDS             complement(17599..18456)
                     /locus_tag="PKN91_04635"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HNS27393.1"
                     /translation="MTTRQLPGAPAGRPQADVRPHLAPAALDRWDAGVRAAADDEDRS
                     ISVYDVIGYDYWTGEGVTAKRIAGALRSLGKGPVTVNINSPGGDLFEGMAIYNLLREH
                     PGDVTVKVLGVAASAASIIAMAGDTVQIARAGFLMVHNCWVMAIGNRHDLREVADTLE
                     PFDTAMADIYAAHTGKDVKAMAKLMDAETWIGGSAAIEQGFADELLPSDQIASDGEKA
                     KASAVRRIEAAMRASGMPKNEAVRLISDFKSSLRAGGVTGDPSATGEGDPAELRESAA
                     IAASLTTIL"
     gene            complement(18460..19755)
                     /locus_tag="PKN91_04640"
     CDS             complement(18460..19755)
                     /locus_tag="PKN91_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HNS27394.1"
                     /translation="MKTKTAKKPGRIRAAILNWLDVPGTLTDADWWAPLSIRSDSGQA
                     VTPTTAMQLSAVWACTRLIAETISTLPLSMYERTSAGKRVARNHALQFVIHDQPNVDS
                     TAAVFWESTVAAMLLRGNAYVEKLMADSRLVGLQFLAPGRLSVTRGLEGRKEYRYTED
                     NGRQRVIPRDRIWTIPGFTLDGKCGVSVVQYGANVFGAAQAADVAAGKTFANGLMQTV
                     YFKMERVLSEKQRTEFRDNLKEITGAINAGKSPLLEGGMEADTLGINPNDAQLLESRG
                     FSVEEICRWFRVPPFMVGHAEKSTSWGTGIEQQMIGFLTFTLAPWLRRIEQAISKDLL
                     TPAERLRFYPKFAVEGLLRADSAGRSAFYTAMVNNGVLTRDEVRELEDREPMGGNAAV
                     LTVQSAMTTLDSIGLGVDDANTARAAIRAFLGLADDRKD"
     gene            complement(19757..21490)
                     /locus_tag="PKN91_04645"
     CDS             complement(19757..21490)
                     /locus_tag="PKN91_04645"
                     /inference="COORDINATES: protein motif:HMM:NF015321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HNS27395.1"
                     /translation="MSRSPRGTRSPGSASGQRDHVAIAIAYAREAAADKRGKKFGKWV
                     RLAARRFLDDLKRSKRKGAPFRFCAWHAADPCRFIEQLPHVEGRWETPNIVLHPAHVF
                     ATVNIFGFRNPDGTRRFSEALFAVARKNAKSTWAAGVLLYCECCEGEVGPQVVSAATT
                     GAQARIVFGIARRMVERTPDLRIAFGVEPFANAIACLSNGGTFKPINAKASTQDGLNP
                     SSAGLDEVHAHKSHDLLNVLKSAAGARANPLFLFTTTEGYETPGPWPELRHFAQQVLE
                     GVIEADHLFALIYAVDEDDEDFDDTKWIKANPLMDANPLLAREMAKLAIEARAMPGRL
                     GEFRIKRLNRQASAARALIDIARWKRCDGAVDLDWLAQFPCWAAFDLSSTTDLTAWRL
                     VWRVEGRWYTWGRRWVPADAVAHRTERGAHAYAGWVAAGLIEQTHGDTVDYAVIEAAI
                     REDAERFRPTAIAFDDWNSRDLCNRLIADGLPLVKFIQGARSYHPAMQEVEQAYRKGY
                     LAHSGDPVLRWCASNVVPRYDANLNMAPDKKRSPDKIDDMVALYMAVGVALAADDEDD
                     MGDFLANPVIV"
     gene            complement(21435..21860)
                     /locus_tag="PKN91_04650"
     CDS             complement(21435..21860)
                     /locus_tag="PKN91_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27396.1"
                     /translation="MGRKAKPPALKLLAGTSRPDREPADAPSFEPVREFPDPPQHLNP
                     DGAQAWNDLGPKLVAAGVLQEVDLYPLEQVCYAWQRFRQKAKAGMDMSASDDNALKAL
                     WSEFGMTPAARRRVAANLVDPNVEKPKGNAFAGIGKRPA"
     gene            complement(22049..22417)
                     /locus_tag="PKN91_04655"
     CDS             complement(22049..22417)
                     /locus_tag="PKN91_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNS27397.1"
                     /translation="MPRKAPTFRPRNITAPKHNPQGKQATNRQRRRAFHTGSKAWRQM
                     REVILTRDGFVCAVCGCYGDQVDHVDGDSHNNDPSNLQTLCITDHSKKTRLEMQAPAP
                     QTASQALSAESTSSNRRCSR"
BASE COUNT         3478 a         7693 c         7296 g         3960 t
ORIGIN      
        1 aacacgcgtt atcccgcaaa tccgcgggat aacacctgaa tccagtggat aacaccgcat
       61 gagcgacacg aacaaaatcc ccgcgcgctg gtactgcctt ggatcgacag gcacggcgat
      121 gctgtgcaag gacgaggaag acgcgcgcaa taaagcggcg ttcgccgaca acgcaaggcc
      181 gcatcacgcc ccgtaccacg ccgtgcgact cgggccggtc gatgagctga cagaggcgat
      241 gacgaggatg tcgaatgctt tgttgggcga cgccgccgac gaaatcgagc ggctgcgcgc
      301 agaactggcc gccgtgcgcc gctaccaagc cggcaacccg ctgggcggcc cggcgtcgat
      361 gttcgccacc gctgcgtcgc gcatcgaagc cggcgagtcg ctggatgatg tgctgcacga
      421 ctacgggtgg tgtcaggagg cggagaggga caggctgcgg gagggcgcac gctacatggc
      481 ccgcgtgtgg attctgtcgg acgacgaccc gcaatctttg gaggccgttg acaactgcgt
      541 cgatggctgc tgcgttgcgg ctgaaacacg caaccgggag attgccgcgc tcgacggcgc
      601 ggcgggaggt ggccatgacc gccgctgatc ctcttgtgca acggttgcgc gccgccgctg
      661 cgcttccgac tggcctgtac gccgaagccg ccgcccgcat cgagtccgac gccgcgaaga
      721 tcgctgcgct ggtcgagcag catcaccgcg acagcgccga actgcgcgcg ttgtgcgagg
      781 ctcgcgacga ggcgaggcgg gagcgcgaca ggctgaccgc ctagagggcg ccacgttttc
      841 aaggggcttg cagcgatgct gccccttttt gctgtccaat atctgggcgt ctgtccaaaa
      901 cgcccggcgt cggccgccac gcaggacact gggcgaagcg gccaaggaca ccggacaagc
      961 gaaggatgcg cggcggtggc gcaccgagga atgggcgatc gcggcgccgg cgtgtagggg
     1021 atttcctacg accccgatga atccgtgact gcctcgatcg gcaacagcgc cgggggcgcg
     1081 atgaactgtg ccgacatcac gaaccgcggg gcatctccaa caatcgggcc gggcgagctg
     1141 caatagccga actcggcatg ccggttgacg ttcgcgagcg tgatcggcgt gcgcgtgaac
     1201 ggctcgccgg tgatcggcac gacgacgaca ggctcgtatg ccagcatgcg ctcgcgcagg
     1261 tcggccaggc ttcccgtgcg cgcataggcg tcggtgtaga cctgcggcgt gaactcaagt
     1321 tgcgacagcg cctctgccct gcgtcgaacg gggaacggct ggccgccgat ggacttgttc
     1381 agacgcgaca ggtccgaccg gctggattcg tactgcggcc ggatgcacca agcttcaccc
     1441 tcgccgatcc acgcctcgcc gatcgagaac gtcgaatcag cgacaatggg ggacgacccg
     1501 ccgacatcgt tgtagatggc gaactcagcg ccgatgcacg gctccaagcc ggcatcgaac
     1561 gggaaccacg cgacgcgcac gccgtcctgg cgctgtacga cgcggcactc ggcggtcacg
     1621 tagtcgaagt tcgcgtcggc cggccggcgc aggccgcaca cgatgcgcgt gccaacgggc
     1681 aacgtgaggc ccacgaggcc gcacaggcgg atcgtggcgg ctgccggcca gacagcctgc
     1741 atgtcgagca cggaagcggt cgtctgcgcg ccagagagcc actggatgcg cgtgccggac
     1801 tcgggccgtc cgttggtcag ccggtcgtcg tcggtcagga atgcggcacc cgttccgacg
     1861 agcgtccacg cgatcgattc gggccgtgag taggagatca gcatttcagc ccctcagggt
     1921 cagcagtagg gtttcggcgc cgagccggcg ggacatgcct gacaccagca gattcctgcc
     1981 ggcgccgagg ccgtgccgcg gatagaccaa ggtccagact tggccgatct gaaccggcag
     2041 ttcggcggac acctcgaacg tgtagatctt gcgcagcgtg ccgtagtagc cggccacgtg
     2101 atcgatctcg gcctgagcgt cctctgcaac gtcgagcagc acttcggccg ggtctgcgtt
     2161 gaccgcctgc gcgtacgtgg cgctcagtgg tgttgccgat gccgcgatcc cctgaaaccg
     2221 ctgcgacagc gcctgccgga cggccagcgg cacgacgaca aaatcggtca cgaagtcgcc
     2281 ctcgccgaga acggtccagt tctttcggaa ccgcatctgc gtcgaaaggt tcggcgcgag
     2341 gtcggggcgg acgatgggct cggacaacat ctcggctgcc gtgatctcgc ccacggcttt
     2401 ctcgtcctcg ggcggtgtca gacgggtgaa tcgcagcacg ccgtcgctgt cgacccacgc
     2461 gcatgcgcag taggagttca gcacggattc cagcgcggcg cggactgtcg tcggctccga
     2521 tgcgtagaac ccgataccgg cgtatccggt ggctgcgtcg atggcctcgg tgtcgtcgcg
     2581 cgaccacgcc gatgcaggaa ggccggcgcg gtcctcgacg atcgagcgca cgaagtcggt
     2641 cagggtgatc gcgttgatgt tagacggcgt gtaggtgtcg gggatttcga ggatgtacgc
     2701 ctcgcggatg atcgcctgcg tgccatgaag agcggttccg aacacgagcc ggggaacgat
     2761 gttggccgag gcagtgatga cgccctcgta cgtgcccgaa gtcgtccacg agaccagcgg
     2821 gattccgccg gccgtggtca atgcgacgta gggctgcccg aacgagtggg gcggcgcgat
     2881 gtactggacg acgacgcggt aacgatagtg cctgccctca tccatctgcg cggacgtgag
     2941 cgcgacgcga ccgacgccgg cgagactgga ggatgtgaac aggagcctgc cgattgctag
     3001 ataggcggcg tccacaacat catcgaaccc gatcaagtcg ccgttgctgt cctccgcgaa
     3061 cggcctgccg taacctagcc agatgtcatc cgccaggctc ggcagagtgc cgccgcccgt
     3121 gctggatgcg tccgccgtca gctttccgac cggttctgtt tcgaggacga cgccgcggaa
     3181 gtcgtcgctg atgacatatc ccggcgggat gtcattggga tcgagcggcg cccccatgtc
     3241 gcggatgtag ccccacccga cgattgcgcg atcgctgagc gcgtagatgc ggttcaccgg
     3301 gtcgacaagc accggcgtga cgctgcgcgc ggcgccttcg aggatcggcc acggcttgcc
     3361 ggcgacgacg ggatcggcgg atggagggaa gatcatgttc tgcagcgggg cgtcgagcac
     3421 ggacatggcg gacgaaaggc gcaggcgcag cgcgccgtcg tcgagcacct gcaggtcgtc
     3481 cacgatcatg tctgcgacgt gatcggctga caggatggat tgcccttgct cgatccattc
     3541 gagcgtcacc ggcaaatcgc gcaggtcgaa gtcgagcaaa ccgtcgagcg cgccgtcgcc
     3601 gttcgcgatc gtgatgttga ccgcgctgcc ttgcgtctgg ctgcggccgt ccggccagaa
     3661 ggcaagcgac tggacaatct cgatgttgtc gtcgcgcagc cggccctcgt agacggcgtt
     3721 cgggatcgga tcgtctggcg gcaggatcaa gtcgcgcgtg ctgacgcgga tcgtcggaac
     3781 ttcgggcggg tactcgaacc atccttcagc gccagcgacc gggaactcga acgcgcgctg
     3841 gccggtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnactc gaaccatcct
     3961 tcagcgccag cgaccgggaa ctcgaacgcg cgctggccgg tgttgagcca cgccttgatg
     4021 atgtcgcttc gcggcgcgct gacggaagca gcgagatacc atggcccggt gctcggcagc
     4081 gtcaccgtgt gcagcgcgat gccgttcaag aaccacgtca ccgttgcggt cgtgtcgatg
     4141 atgttcagcc acacgccaac gatgtcgccg ggtccgccag cagtgaccgc ctgcacgctc
     4201 gcgcctgcgt tgtggatttg cccctcggcc acgcggtagc cgtagccatc cgcgtcgccg
     4261 ccgacgtagg tggacaggct cgcatcctcg gtcacgaggc cgaacgacac cgcatcgctg
     4321 atgtcgtcgt cgccccacgc ggcgaactcg gcgtaccacg agaacgcctg cttcccgtac
     4381 aacgcgcgcg ccgtgcgatt gatgtcggtc gtcgcagtcg gcaggacgat cgtgttcgcg
     4441 gattcgagcg cgagcaacgg cccgagcgcg gcggggtcga atgtggagta gcgaaggccg
     4501 ctcacagcgt aatgatcaac gcgaagccat tgccgccatt gccgccacga cccgagttca
     4561 ctccgctacc gccgccgccg ccgccagcac cgagaccgcc atcgccgccg cgacctccga
     4621 cagtggaact cgacccaccg cctccgccac cgccaccact gcctccggca tacaggaggc
     4681 tctgcccgtc tccgcccgca ccgcccggag tcgacccatt gccagcggtt ccggccgccg
     4741 gtcgagtcca gtcggatatc gtggccccgc cttgacctcc agcgcggcca gtggctccgc
     4801 tgtgtccgcc gccgccgccg ccaccgccgc cgccgtacat cgcctgacca cccctgctgc
     4861 cgtcgtaacc agaatcactg ccgcccgcgc ctccggccgc gccgcccaac gggttttttg
     4921 catgggtgtc agactctccg gaattgttgt accccccgga attccctccg ccaaaagttc
     4981 ctcccgtcga accgatcgcg cggcctcccg cgcctgcccc accggcgcca acggagtgat
     5041 atcccgcgcc gacgccattg cctccatacg caatcaatgt ggaaaacgtg gtgtcgccgc
     5101 cgtcagcgcc agcgttaggc agatcgcctg acgtgagtgc ggcgccgccc gcgccgcccg
     5161 cgccaatcgt gacggactcc gtcgccccta gcgcagacgc cggcagccag ccctcaatca
     5221 tcgcgccgcc gccgccgccg cctactccat ttgccccgct gccgcggctg ccactggcgc
     5281 cgccgccgcc acctccgagc aagcgcacat agacatagga tgcaccatcg ggcttcgtcc
     5341 acgtgtgcga tccaggcgtt tccgaatacc aaacgttcgc accgccacct ccaccggcga
     5401 gtgcagcgac gtctgccagc gtcgttttaa cagtgacgcc atcctgcacg actggcacca
     5461 gctcatcgcc gtccagagga tcggcagatg ggagttcgga gatttttacg tcggccattt
     5521 attcagtcct cagaaggtcg ccggattcgg cagcgatggc gtcaccgacc tcggtcatga
     5581 tgtgatacga gacagaaacg atgctgatcg tgtccgtgat ctgcgcgtac gcagccgtag
     5641 gcgtctgcgc gtcccacacg ccgatggtga acgagtgatt gactgcgctg acggtcggcg
     5701 tgccttcgat cgtgacttca tcggcgccag gcccggatgt gatgtagagc ccgtccggaa
     5761 ggtagctgct gatgagcccg aacatgtacg gctgcacgcc gcccgagacg gtgtaggtgt
     5821 agctgtaggg gacacccacg tcgccatccg gcgcgctgcc cgcgatgacc agcggttccg
     5881 cgacaacccg cagcttgaat tgccgcgtga ccgacttgcc cgacgagtcg cgcagaatga
     5941 cagtgatcgg gtacaggccc acggccgagg tcgtgccgga cagcacgaaa tcgccgttgc
     6001 tgtccacgcc gaacgaaagg ccggcaggca ggaagccgga tacgagcgcc cactggtacg
     6061 gctcgattcc gccgtatcca accagcgtag cgctgtagga aacgccatag ccaacaggcg
     6121 gcaggatgcc aacaatcgct agacccggat tgatcgtaag ggcaaggatg cgctggtact
     6181 tcatctctca agtacccaag cgacacgccg gcctccgctg gccctgatct ccaggttcag
     6241 ctctcgcgtt tcctgcgcca tccccgacag cgcgccgccg agcgttgcgt cggtctgcct
     6301 gtcactcgac acgattgtgc tcgccagcga gtcgatgcgc acagcaatgg attcgagctt
     6361 ctcggcaacg atcgtggtcg attccatctg ctcgctgagc gatgcagtcc gcccctcgat
     6421 cgcgtccacg atgcgatccg tgttagtggt gatcgagtcg tcgaacgatt cgctgattgc
     6481 cgaccagtcc tgcgcctcga attgcgcgac cagcgattcc aggtacgcct gcagcttctc
     6541 gtcggtgagc gacaggtcgg cgccaagctg gcccaacgtc cagcccatcg actcggcgag
     6601 ttcggcgaag ctgtcgcccg tggcgtagga caactctgcg atccggcgcg cgagctggtc
     6661 ggcaatctca ggccgttgcg cagccaccag cgcatcacgc gcggcgatga gttcgtcgag
     6721 cgatcggcca tcgccagagc tgccgacatt cccgcccggc gtcgtcgaac ggaagttcgc
     6781 catgcccatg acctgattga acaggttggt gtagttcgac gtgctggcgt acaggcgccg
     6841 gccgatctcc agcacctgct gcgggtcgac cgtgccttcg cgcaatccct gcaacgcgag
     6901 ttcgagcttg cgctggtcgt tgaacgggga gaggtcgccg agcagcaggt tcatcttctc
     6961 gcgcatgccg tcgatggcct gcccgatcgc atcggcagca tccccgctgg cgccggaaag
     7021 cgatgcgatc tgcgcgttca gggattcgat cgtgtctccg ctgccgcccg tgatgtagcc
     7081 gagcgtggca gcaaggtcgc gcgattcgtc cttgagcttg gcgatcagtg ccgcgatgcg
     7141 ctgcgcagcg acggcatgga cgttcacaag gtcctgctcg gatgcgcccg cgcgccccgc
     7201 ggcacgcgca aggtcgttca gcgaacggat cgtgtccgtc gtccacttct cgatctcagc
     7261 gcggccggcg atgaacccgc ccgcctggcc aagctcgacc atcagctcgc ttgccttgat
     7321 cgcatagtcc agccgagcct tctcggcctt gtcgagcagg tcgaagtagt cgcgcgccac
     7381 gccggcgagg cggatcagcg ttgcgatctg ttcgcggcct tcctcggtcg tcgcgtcgaa
     7441 cgtctgcatc aacgcccaca tcgcgtcgcg cgtctcgggc agcacgaggc cggcttgctc
     7501 gaaggcggac gtgagcgatc cggcggcgac ttcgagcagg tgattcgccg gagcgaacgc
     7561 acccatgaac gccgtcatgc cctcgatcat ggcgtcgatg ccgccgacga gttcgagcag
     7621 cccctcgctg atctgcgcga acttctcggg gtcggtttca tcgagcgcga agccgagata
     7681 gcgcacagct tcctgcgtca cctgcacgcc ggtcgccacg cgcaccaacg tctcgcccaa
     7741 gccctcgccg acttgctgga actggtcgat ccacggcacc acggcaccgg cgaggccgtc
     7801 gaacaggcgg ctgaataccg cctcgatctc cttttgctgc tcctcggcat cgaggccctt
     7861 gaggctgatg cggatttcct cgacgcggaa cgccgccagc gccgcttcga cttcctccgg
     7921 cagcagcccg agcgcgagcg cgccttcgcg caccgtgtcc gcaatcgagc caatcacgag
     7981 ctggaactgc cgcccgaacg tgtcggagat gtcgatgatt cgatccttga tcttgtctct
     8041 gccgaacagg ccgccgtccg tcctgatcgt ctggtacgca cccacgacga tgctttcgag
     8101 catgtcattc agcgcgccac cggcgatgat gattccattg tcgacgatct tccggccgcc
     8161 gccgaagatg gcgttgctga gcgatcgggt gagcgggtct ttgccgagga tgtcgaggtt
     8221 gaacccttgg ctcaggccgc cgaactccac atccgccgcg ccgcgcgcaa gctggttgcc
     8281 ggccgcgccg agcgcgttct gcagcgagtg cagcgcgttc agcatccctc gattgatgcc
     8341 gacgagctgc tgcgtggcgt tcgccgtgat ctcgacggcg ttgaggatgc tctcggactt
     8401 cgcagtcgca tcgccgagga tcgaaccggt gccctgcgag gcctgccggt tctccgacgt
     8461 catgtccttc ccgccaccgc cgccgaaact cttcagcgac aagccgatgc tggcaatgaa
     8521 cggagcgacc gcagcggcca tcgccgccat gcgcgcccat gcgctgtacg gttcgccctg
     8581 gccctgtgtc agcacggcgg ttacagccgc cttcaacgcg atgatgtcct gcacgatctg
     8641 cagggcggcc atgcccttct cgatcgcctt gaaacccttg ctaccttcct tagtgaaggt
     8701 ctgcatggcg ccgagtagcg ccttgtacga tccgagcgtc tgaatcgcaa cctgcgcgtt
     8761 caacttctcg acggcctttt gcatcggcgc gataatctcg gggtccatcc cctcctcgaa
     8821 cgccgccttg atcgcgtcgc cgagttcctt ggcctgatct accatctcca agatcggcga
     8881 cttggaggcg tcattgatcg ttgccgcttt gctcaacgcc tcgttgatct tcgccagcgc
     8941 gcgaaggcgc tcggtgtcga ccttcacgac ttcctttccg gccgcagcgt tcaaccgctt
     9001 ggcgtccgca agcgcgcgca tgacgatcgt ctctgcgcgc tgctgtgccg ttgtcaggcc
     9061 gatcaggcgc gcttcgtcgg caagctcggc gacgtactgg cccgttacgt cgcgctcgcg
     9121 ctcgatggcc gcaatggttc gctcgcgcgc atcagcagcc gcgttctccg catcgtcaac
     9181 catgcgcagg atttctgcct gctcaattcc acgcgcgcca agctttgcgt tctcggcctc
     9241 ggcctttgcc gcggcatcag cgatgtcgcg catcgtcttt tcgtaatctg cccacgcctg
     9301 gtctaccgga tcgacggcgg taagctgcga ggtgatgtca acgatcttcc gaatcgcgga
     9361 ctccagccca tcggcggcct ttgcggcagc cgccatcgcg ccttcgtcag cgaatgcctg
     9421 catgttcttg cgcagcgtct ccgtgctgcc ggcggccttc tcgatcggct tgtcgatcgc
     9481 gtcgagcgta gcgttcatgc cggcaagcgc gacttcgatg ccatccgttg ccgtcgacca
     9541 tgcggtcgag atcgtctttg cgccctcggt ccaaccctcg gcgaagttcc cgcgcatctt
     9601 catgaacgat tcgttcgcgc cggcaaagtc tcccgtcaac tgcttgagcg aacccttgat
     9661 tccctcgacc acgccgccga cggcgccggc tgccacctgc ccaacgccga cgatgctgtc
     9721 gaacgtggcc gcaacgaggt tcgtcagccc ttcgatcacc gccttcagca cggtgaacgt
     9781 cttggcgacg tactccgcca ccgtgccaag ccactcgaag ttgttcccgc cttcccgcgc
     9841 tcctaccccg gcatcgacga acgcctcggc gatctgcgtc agcttcggca gcaaggcttg
     9901 cgcgatgtcg ttgccaacgc cgcgcacgag atcagccatg tcgcccatgg cgtcgttgaa
     9961 gtcgccggcc tgcgccgctg ccgcatcgga gatttcaaga ccaagcgcct ttgcgcggtc
    10021 ctgaagttcc cgcatcccat ccgcgccttg gttcaggaac ggcagcatgt ccttgccgga
    10081 ctttccgaaa atcgccatgg caacggctgt tttctgcgcg ctgtcctcgt acttggcaaa
    10141 ttcagtcgcg gcctccttga gcagctgctc cgtcgacttc agcgtgccgt ccgtgttctt
    10201 gacggagatg ccgagatgct cgaatccctt cgccgcggtg cccgtgccgg cagccgtgtc
    10261 gctggccgac ttcgccagct tgccgagcgc cgcttccagc tgctcggacg acaccgcacc
    10321 gcgcttggcc gcgtactcaa gcgtcgagag ctgtttcgcc gacacgccca cgcgctgcgc
    10381 agcttcgtcg aggcggtcca tgttgtcgat ggcgctcttg accagcgcag cggttgccgt
    10441 ggtcacggcg gccagtgccg ttccgatgac cttgccggcc tcctcgaggt ccttctgcat
    10501 cttcttcgcg gcgcgcgcgg tctccttctc cgcgcggccc atatccgtga tgaacgtgcc
    10561 ggtgagggca cgaagcgcga ttgagataat cacaggggag gactccgatt catcagggcg
    10621 gacaacatca gccggtcgga ttcgctgtag cgatccgatg acggcgttat ggcgggagcg
    10681 gccttccagg cgtcgcgcgc tggaaggaag tcgacgagct ttgccggcgg tcgatctttc
    10741 ggccggtgcg agttggcgta catcgttgcg atctgcgcga gcgccagctc gatgcgttca
    10801 tcaggcgccg gctcgcgcga caggtacgtc gcgatcaggc gcacatggga caccggccaa
    10861 tccatgacgc gcgcgagcgg ctcccggtag agcaccgaga gccgcacgag cgtgcgaagg
    10921 aacggatcag ccgtcagccg tttccctcgg cttctccggc gggcaacgcg tcgcccgcat
    10981 cgtcgacatc ggccctctgc gcctcttcgg cgagcttgcc gagcgcgatg atcttgctcg
    11041 cgggctcggc atgaagctgc ttgatgtgcg catagacctg gttgccgttc tcgtcaacga
    11101 gcgtcatcag cacgagccgc tgttgcgact cgacgttctc gccgacatcg atctcgacgt
    11161 tgcccgactt cgcattgccg cggtacgtct ggcccttcac gagggacagc tgctcgccag
    11221 ccgtgagttc gcggaaatac accgtggtct cggtgccctt gtgcttcagg gttttcttga
    11281 ttagttccat cgccgttgcc tttcaatggt cgcccggaac gacgcgcgga tacgcacggc
    11341 gaacgcgcag agccgcgcgc cgcccggacg gttgagtgga gaggtggacg ccgttgagcc
    11401 ggttacggca ggtcggcagc cggcagatcc caggccagct cgctgttgac ctggatcgtc
    11461 acggtgccgc gccagatgtc gcccacctgg gcgttgatgg cgaagttcga gacatagccg
    11521 cggaacccga cggtcgtcga gccgggcgac agcagattgc catcgctgtc gacgctgacg
    11581 ggcagcgcgg cctggtcgct gaacacgacc atccacggca tgacaaggtt ggccccctgc
    11641 tccttcgccg cgatgagcgc ctgatgcgac tcgctgcgcg ggatgaagtt gatcgggatc
    11701 gccagttcgc cgggatcggc gaggccgctg aagtactcgc gcaccggcga cgagaggcag
    11761 gtcatgtcct gcttcggccg cgcgccggag gtgaggtcgg ggatgccggt cgggcacgcg
    11821 acctgcagaa cgatggtgcc gtccggatcg gacgacgatg cacccggcga atagccgaag
    11881 cagagccggg tgtacttggt ttcgattacg ccttcggtca tgacactttc ctcatcgatg
    11941 ttggcgggga gtccgccggt atatggccga gcgctcggcc aaaacgcaga aggcccgcgc
    12001 atggcgggcc ttcgtgatcc gtgtttctgg tctcgtcagg tgctcgagct gctgatcgag
    12061 tcgctactcg aggcgtcatc ctcgcggtgc acgaacacgc gcacgtcgaa gtcaatgcgg
    12121 tagcggccgg tctcgatgtc cttgcgcatg tcgcgcacgg cctcgacgtg gtgcgcgcgc
    12181 tcgatgcacc ggcgcgccgc ggcggcgagg tcgcgcagtt cgtcgacggc gtcgccgccg
    12241 ttgtccgacc agcacgacac ctggacgagc gtcgagtccg cgcgagcggc gccacggtcc
    12301 agcgtgttga tcggcaggat cgtcgaggcg aactgcgtca ggtacggtgc gacgacgccc
    12361 tgcggcgcgg tgccgtgcgg gtagaaccgc accggcgacg tgccgatgaa cgccgtcacg
    12421 gacgggtccg cggcaatcag cgggaacagg ctcggcaggt tcatcccttc gccctcgcct
    12481 cgcgctcgac acggtcgatc accttctgcg ttcgcgcgcg catctccgcg acgaacgtct
    12541 gcaccgcggc gcccttcttc gcatcgaacg ccggtcgcag ccatggcatc ggctgccggc
    12601 gctctgtgcc gtactccagc agtcgcccga cctgcacggc cgtgatgccg cctttcctgc
    12661 cttgtcggcc ctgcgggtat ctctgtccgc gcttgatgcc gacgacgaag gcctcgccgc
    12721 gctcgccacc gcgcatgcgg ctgcgcttcg cctggatcga cttcatcagc aggccggtgc
    12781 tttcgttctc gccgctcttg ttcggtgtgt cgatgatgcg ttgcacattg cgcttcgcct
    12841 cgtcgcggag gacctcggcg gccttcttca gtgccagctt cacggggccg ccggctttcg
    12901 agacgatctc gggcggcagc ttgcgcagcg catcgaggac gcccgccaag ccttgcaccg
    12961 atacggtttc aactgccatc agtgagcccg tccttgcagg tgatgcggta ctgcctgcgc
    13021 gcagtggcgt cggtctcgat gccggtgatg tcgaacacct tgccatccca caggatgcgc
    13081 cacgtcggca gcaggccggg gaaccacggc atctggatgc gcgctgtcgt ctcggcctgc
    13141 ttcgcgtcgg cggcgtagaa ctcgcggccg cgcccggtca ggacttcggc cggcacctcg
    13201 ttgagcggcg tatcgctgtc gagccacacc gtttcccagg tgtagggcac ggcgccggtg
    13261 cgcgggttct gtgggcccgc gaccttctcc tggatgttca cgcggtggcg gtagcgttgg
    13321 gcgtccactc agacccccag cccgcgccgg tacggcatca ggacggtctc ggcgttgcgg
    13381 cgcatgcgct cggccttgtc ggggtccgtc tgctcgtagg cggcctgcac gaggatgcag
    13441 acggccttct cgaacgacgg cgcgatcgga tcgtcgctgc tcggaacctg ctcgctttcg
    13501 cactcgctcg ggtagtcgag cgggagcgtc ggcggctccg tgcggttcag gaagcgcagc
    13561 gcctcgtcct cagcttggtc gatcatctgc tgcagcaggg gatcatccgc ggactggaac
    13621 acgcgcagcc acgccttcac ggtggcgagg tcaacgaaac tcacggtagg cctccacgtc
    13681 gcttccgatc cagcgttcga gcatgcgcag ccctgggtcg cgcgaaccct gtggctggtg
    13741 gtggcacggc atgtagccgc cgcgtcccgg cagacccttg atgccgacca cgcggccgtc
    13801 ggggaaatag cgctttcgcc ccgcgtacag ctcccacccg tcgcggtcga cgtaggcctc
    13861 gcggctcgcg gcgatcgcgc gcaggtgctc gatcacgcgg ccgttgaacg ccatcgacga
    13921 cgtccacggc cacgcagccc ggtacgcacc gacacgctgc agccgcacgt tcgcccaccg
    13981 ctgcggcccg acgccgacga gatcgtgctc gcgcaggcgc gcggcgcagg tctcgaggta
    14041 gtcggccgca taccagtcgt catcctcgac gacgacgacc ggcgcctgca gggccgcgtc
    14101 gaggccggcg acgaagttcc gcggctgcgt gcgatcaccc tcgcgccaga acggcatcgg
    14161 ccgaaccacg tcgatgcacc agttctccgg cagctcggcc ggcatcggcg tgggttcttc
    14221 gccgtcgtcg accacgatcc atcgaacctg tccgcggaac gtctgccgcg ccatccagcg
    14281 tgcgcacagc gcgaacgcct ccggccggca gccggttgtc gtcaggacct gaagcatgcg
    14341 tagcgctccg cgtcatcgcc aatccacgat cgcagcacgg acaagtcatc gtcctggtct
    14401 ccggcgaagt cgtctctgtg cccgatgcca atacctccgc ggcccggcat gcccttaatc
    14461 cccacgacgt gcgtcgcgac cgtcaacatc gccggcaccc ggcactcgcg ccagagccgc
    14521 atgtcaaggc cggacgcgtg tcggcccgcg aggctgaaca gcattcgcgt cgcgcgcccg
    14581 atcgtcgccg tgctgcacag gctcgcgtgc gcgacgttgc ccatgttccg cgcgacgcgc
    14641 cggcggacgt ggtagtaccg cgcgccggcc tcaccgaaca gttcgcagtg cgtgagcgca
    14701 tccgcgcagg tctgaagcca cgccggagaa taccagtcgt cgtcctccac gatcaccagc
    14761 gggacctcgc cgtcgatcgc cagcaggccg gcgcgcaggt tccgcgcctg cgtgttctgg
    14821 ccgggctgcc acaccggccg cgtgcgcatc acctcgacct tccaggtcgc cggcatggtc
    14881 ggcgcatcga tgcccggctc gccgtcgtcg acgatcaccc agcgcacggg ctcggtgtac
    14941 gtctgccggc gcatgtactc gacgcacatg ccgaaagcct cgggcctgcc gcccgtggtc
    15001 gtgagtacct gcagcgtcat gcgcgcgcga ccgcgaaggt gtgcagcggc tcctgccgcc
    15061 ggatcaatcc gcgctcgccg tggtcgttca gggcgaatgc cacgttcggc ccgctgttcg
    15121 ccgtcgcgac gtccttgtag ccggacacct cgagcacggc gcgcagaccc gactccgtga
    15181 accggtagaa gtcatcaggg aacgcgtgcc gcgggaagca gaacgggacc gtcacgatca
    15241 gcagcgcgcc gggctgcatc agcgcgcgca gccgcaccag cgcggtccac gggttgcgca
    15301 cgtgctcgag cacctcggac agcacgacgc cggagaaccg gccgatccac tcgtcgggcg
    15361 ggtcatgaag atccgcgacc acgtcgacgt tcgggcccgg ctgcatgtcg atgcctgtcc
    15421 actggccgcg ggcgaggtcg cgattcacga cccaccaggc gtccggcgtg tgcatgcgcg
    15481 agccgacctc gagcacgtca tcgccgaggc cggctgcgtg cttctcgatc cacgcgcgga
    15541 cctgcccgcg cacgctattc ggtggcagcc tgttcaagct gttcctccaa ggttccacgt
    15601 ggaaagcacg tcagcgcagt ggcgcgcgac gcgttgatga cctcgacccc gagcgcgcgc
    15661 atttgccgcg agcactgtgc gaatcgctcg aaccactgcg gcatgctctt cgcgttgccg
    15721 aggctcggcg ggtggtcgcc gtgcgaatgc gacttgccgc ctgttcgctg gcagtcatag
    15781 ccggccagga cgatgcggcg cgcacccatc caggccgaca gcgcgatcgc cgcggctccc
    15841 gagttgctga acgtctggaa cggactcgcc tgcgcgaacc cgtgttgctg ccggccgctg
    15901 aagcgaaggc cacggaacga ttcgatgacg tcctgcccgt agaccttcca ccacggcggg
    15961 tcgatcgcat acagcgcatc ggcccaaggg gcggccttga acatcgtgtt cgtgacgact
    16021 acggctcgcc ggccggttcc tccggatcgt ccttcgcctt cttgcgcttg cccttcgccg
    16081 gctccgaatc gccagcgtcg gattcgctcg acgtcgtctg cggtgcagct tgggccggaa
    16141 gcgatgcaga cgacggtgaa tccgtcccac cggcctgggg agggtgttca tcggccggga
    16201 ggccggagaa gctgacgagg cccttctcgc gcagcgcgcg cgccgcggtg ctcgtgatgt
    16261 tcggtgtgtc accccgcttg taggtgacgc cacagtggtt gaacgtggaa agtgcggtga
    16321 cgatcatgct cggctccaaa gagaaagggc ggccgaagcc gccctttcag gtggttgcac
    16381 tggttcgacc ggatcagctc aggtcgtcga agccgccctt cacaaacgcg gccggccggt
    16441 agaccgtgag accgatccgc tcttcgcagc ggatggtcgc catgttcttg atgaagttgt
    16501 cgcggtcctc gagcgagatc acgacgttga cgtcctcgcg atcccagccc tgcaccgcca
    16561 ggccaccgcc gaacgcaccg acgaggaagt cgtcggccga gatcgcctgc gtcggaacga
    16621 cgttgcgacc ccacaggccc ggcaagttca ccgaacgcgg gttggcgaac aggtaggcgt
    16681 tgtcctcggt cttcgtcagc tcgatcgcag cccagtcgat cgggctgatc acgataccgt
    16741 ccgcccagta ctcggcgagc tcgacctgca gcagggccag acgcagacgg tcgaggcggg
    16801 tctcggcctg cacggtcacg cccgggttgc tgtaggccgt ggcctgcgtg tagatgccgt
    16861 cgaggttgag gccgacaccg ctgccgttca gcagctggtt ttcctcgaca agcttcaggc
    16921 cgtagcgcag gcggccgtcg atgtacgact gcagctgcgg cacgtcggcg agcacctgct
    16981 tcgacgcgtg gatccagtgc gcgatcgtca cgatcggggc ctgcgcggct tcgaacgtga
    17041 tgttcgattc cgccttgccg gccgacgggt tctcggacac cggcgcggcg ctgttggtga
    17101 acaccagctc gcgcgcgaac tcgaccgagt tcgaggtcgt gcgaccccac gcgatcaggt
    17161 cgctgatcgt caggcgacgc aggcccggct gctggatgcc cgggatgcga tccggaagga
    17221 tcaggtcgcc cgccgatgcg gcgccttccg tgatcttcgc ctgcacgccc atcgcgaacg
    17281 agccggccgg gcgcttcgcc caggtctgga agccttcgtt cgcggtgacc tgctcgccca
    17341 tcgtctgcgg acgctcgggc tcgccgccgc cctgctcgag cttggcgagc ttctgctcgg
    17401 ccgactgcag gttcgcgcgc agctcgccct gctgcatgag cagttcgtcg accttggcgc
    17461 gggtttcctc ggagagcttg gcgtgcgcct tgatctcctt gtccgccttc tcggcgtggg
    17521 ccttcagctg gtcgccgacc tgcttcaggg attcgttgat tgccttgatg tcgccatcgg
    17581 cggccatggt gctacctctc agagaatggt ggtcagggat gcggcgatgg ccgcggactc
    17641 gcgcagctcg gcagggtcac cctcgccggt cgcggacggg tcgcccgtca cgccgccagc
    17701 ccggaggctg gacttgaagt cgctgatgag gcgaacggcc tcgttcttcg gcatgccgct
    17761 cgcacgcatg gccgcttcga tgcggcgcac cgcgctggcc ttcgccttct cgccgtcgct
    17821 ggcgatttgg tcggaaggca gcagttcgtc ggcgaaaccc tgctcgatcg cggcgctgcc
    17881 gccgatccac gtctcggcat ccatgagctt ggccatggcc ttgacgtcct tgcctgtgtg
    17941 cgcggcgtag atgtcggcca tcgccgtgtc gaacggctcg agcgtgtcgg ccacttcgcg
    18001 caggtcgtgg cggttgccga tcgccatgac ccagcagttg tgcaccatca ggaatccggc
    18061 gcgtgcgatc tgcacggtgt cgccggccat cgcgatgatc gacgccgcgg aggccgccac
    18121 cccgagcacc ttcaccgtca cgtcacccgg gtgctcgcgc aacaggttgt agatcgccat
    18181 gccctcgaac aggtcaccgc cggggctgtt gatgttgacc gtcaccggcc cttttccgag
    18241 agagcgcagc gcgccggcga tgcgcttcgc cgttacgccc tcgccggtcc agtagtcgta
    18301 accgatcacg tcgtagacgc tgatcgagcg gtcctcgtcg tcggccgcag cgcgcacgcc
    18361 ggcatcccag cgatcgagcg ccgctggcgc aaggtgcggg cgcacgtcgg cctgcgggcg
    18421 gccggcaggc gcacccggga gttggcgggt cgtcatggtt caatccttcc tgtcgtcggc
    18481 gaggccgagg aatgcgcgga tcgccgcgcg cgcggtgttg gcgtcatcga caccgaggcc
    18541 gatgctgtcg agcgtggtca tcgccgattg cacggtcagc accgcggcat tgccgcccat
    18601 cggctcccgg tcctcgagct cgcggacctc gtcgcgcgtc agcacgccgt tgttgaccat
    18661 cgcggtgtag aacgccgagc ggcccgcgct gtcagcgcgc agcaggccct cgacggcgaa
    18721 cttcgggtag aagcgcaggc gctctgccgg cgtgagcagg tccttgctga tcgcctgctc
    18781 gatccggcgc agccacggcg ccagggtgaa cgtcaggaag ccgatcatct gctgctcgat
    18841 gccagtgccc cagctcgtcg acttctcggc gtggccgacc atgaagggcg gaacgcggaa
    18901 ccagcggcag atttcctcga cgctgaatcc gcgcgattcg agcaactgcg cgtcgttcgg
    18961 gttgatgccg agcgtgtcgg cttccatgcc accctcgagc aggggcgact tgccggcgtt
    19021 gatcgcgccg gtgatctctt tcaggttgtc gcggaactcg gtgcgctgct tctccgagag
    19081 cacgcgctcc atcttgaagt agaccgtctg catcaggccg ttggcgaagg tcttgccggc
    19141 tgccacatcg gcggcctgcg cggcgccgaa cacgttcgcg ccgtactgaa cgaccgacac
    19201 gccgcatttc ccgtcgagcg tgaagccggg gatcgtccag atgcgatcgc gcgggatgac
    19261 gcgctggcga ccgttgtcct cggtgtagcg atactccttc cgaccttcga gcccgcgcgt
    19321 gacgctcagt cggcccggcg caagaaactg caggccgacg agccggctgt cggccatcag
    19381 cttctcgacg taggcgttcc cgcgcaggag catcgccgcg accgtcgatt cccagaacac
    19441 cgccgcggta ctgtcgacat tcggctggtc gtggatcacg aactgcagcg catgattgcg
    19501 cgcgacgcgc ttgccggcgc tcgtgcgctc atacatcgac agcggcagcg tgctgatcgt
    19561 ctcggcaatc agccgcgtgc acgcccacac cgcggacagc tgcatcgcgg tcgtcggcgt
    19621 gacggcctgc ccagagtccg agcggatcga cagcggcgcc caccaatcgg catcggtcaa
    19681 cgtgcccgga acgtcgagcc agttcaggat ggcggcccgg atgcgccccg gcttcttcgc
    19741 ggtcttggtc ttcacgtcaa acgatcaccg gattcgccag gaagtcaccc atgtcgtcct
    19801 cgtcgtccgc tgccagggcg acgccgacgg ccatgtagag ggccaccatg tcgtcgatct
    19861 tgtcggggct ccgcttcttg tccggagcca tgttcaggtt cgcgtcgtac cgcggcacga
    19921 cgttgctggc gcaccaacgc agcaccgggt cgccggagtg cgcgaggtag cccttgcggt
    19981 aggcctgctc gacctcttgc attgcagggt ggtaggagcg agcaccctgg atgaacttga
    20041 ccaggggcag tccgtccgcg atcagccgat tgcacaggtc gcgcgagttc caatcgtcga
    20101 aggcgatcgc cgtcggccgg aaacgctccg cgtcctcgcg gatcgccgcc tcgatgaccg
    20161 cgtagtcgac ggtgtcgccg tgcgtctgtt cgatcaggcc ggccgcgacc cagccggcgt
    20221 aggcgtgcgc accacgctca gtccgatgcg ccaccgcgtc cgcgggcacc cagcgccggc
    20281 cccaggtgta ccaccgtccc tccacgcgcc agacaagacg ccaggccgtg aggtctgtcg
    20341 tgctcgaaag gtcgaacgct gcccagcacg ggaactgcgc cagccagtcc aggtcgaccg
    20401 cgccgtcgca gcgtttccac cgggcgatgt cgatcagcgc gcgcgctgcg gacgcctgcc
    20461 ggttcagccg cttgatccgg aactcgccga gccggccggg catcgcccgg gcctcgatcg
    20521 ccagcttcgc catctcgcgc gccagcagcg gattcgcgtc catcagcggg ttggccttga
    20581 tccacttcgt gtcgtcgaag tcctcgtcgt cctcgtcgac ggcgtagatc agggcgaaca
    20641 ggtgatcggc ttcgatcaca ccctcgagta cctgctgcgc gaagtgccgc aactccggcc
    20701 acggtccggg cgtctcgtag ccctccgtcg tcgtgaacag gaacagcggg ttcgcgcgcg
    20761 cgccggcggc agacttgagc acgttcagca gatcgtgcga cttgtgcgcg tgcacctcgt
    20821 cgaggccggc cgagctcggg ttcaggccgt cctgcgtcga ggccttcgcg ttgatcggct
    20881 tgaacgtgcc accgttcgac aggcatgcga tcgcgttggc gaacggctcg acaccgaacg
    20941 cgatgcgcag gtcgggcgtc cgctcgacca tccgacgcgc gatcccgaac acgatgcgcg
    21001 cctgcgcgcc ggtcgtcgcc gccgacacca cctgcggccc gacctcgccc tcgcagcact
    21061 cgcagtacag caggacgccg gccgcccatg tcgacttcgc gttcttgcgc gcgacagcga
    21121 acagcgcctc gctgaaccgg cgcgtgccgt cgggattccg gaagccgaag atgttgaccg
    21181 tcgcgaatac gtgcgccggg tgcagcacga tgttcggcgt ctcccaccga ccctcgacgt
    21241 gcggcagctg ctcgatgaac cggcatgggt ccgcggcgtg ccaggcgcag aaccggaacg
    21301 gtgcgccctt gcgcttcgac cgcttcaggt cgtcgaggaa ccggcgtgcc gccagccgga
    21361 cccacttgcc gaacttcttc cctcgcttgt cggccgccgc ttctcgcgcg tacgcgatcg
    21421 cgatggcgac gtgatcacgc tggccgcttg ccgatcccgg cgaacgcgtt ccccttgggc
    21481 ttctcgacat tcggatcaac caggttggcg gcaactcggc gacgcgcggc cggcgtcatc
    21541 ccgaactcgg accagagcgc cttcagcgcg ttgtcgtccg atgcgctcat gtccatgccg
    21601 gccttcgcct tctgccggaa ccgttgccac gcgtagcaga cctgctcgag cgggtagagg
    21661 tcgacctcct gcaagacgcc ggcggcgacc agcttcggcc cgaggtcgtt ccacgcctgc
    21721 gcaccgtccg ggttcaggtg ttgcggcgga tcggggaact ccctgaccgg ctcgaacgac
    21781 ggcgcatcgg ccggctcgcg gtccgggcgc gacgtgccgg cgagcagctt cagtgccggc
    21841 ggctttgcct ttcgtcccac aaaaacccct ttgccggata tcgccggtgc gaaaaaacga
    21901 ttggacgccg cgtgccgtag acgggaaagg gcagactttc gcccgccccc cccggctcac
    21961 agtgtctgga tgtccttgcg catcggcgga accacgcccc cgttcaggtc gaaggtcgga
    22021 gcctcgtcgt cctcgatgcc ttcctcgatc agcgcgagca gcgcctgttc gagctggtcg
    22081 actcggcgct caaggcttgc gatgcggtct gcggcgctgg tgcctgcatc tccaggcgcg
    22141 ttttcttgct gtggtccgtg atgcatagcg tctgcaggtt gctcgggtcg ttgttgtggc
    22201 tgtcgccgtc gacgtggtcg acctggtcgc cgtagcagcc gcacacggcg catacgaagc
    22261 cgtcgcgggt gaggatcacc tcgcgcatct ggcgccatgc cttgctgcca gtatggaaag
    22321 cgcggcggcg ctggcggttg gtggcttgct tgccctgcgg gttgtgcttc ggtgctgtga
    22381 tgttgcgcgg gcggaacgtg ggcgccttgc gtggcatcag cggaccatca ggtccgaggc
    22441 gcggacgggc agcgcgctca tcggatcgcc ccattcgccc ggagcgcgcc ctcgcacgtg
    22501 cgcgcagcgt ggcccttggc gccttcc
//
LOCUS       DAOQVY010000031        22174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_2741_length_22174_cov_5.851078, whole genome shotgun sequence.
ACCESSION   DAOQVY010000031 DAOQVY010000000
VERSION     DAOQVY010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 22174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22174
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2741_length_22174_cov_5.851078"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PKN91_04660"
     CDS             complement(<1..714)
                     /locus_tag="PKN91_04660"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNS27398.1"
                     /translation="MKCGIERLIGEPRLRRGLAGRRAALLAHPASVLPDLGHSLDALA
                     AVPDIELTAAFGPQHGLRGDKQDNMVESTDFADPASGIPVFSLYGEVRRPTPEMMAGF
                     DVLLVDLQDLGCRIYTFITTLRYVLEAAAEHDKAVWILDRPNPVGRPIEGTVLRAGWE
                     SFVGAGPLPMRHGLTMGELAHWFVATLGLEVDYEVVTMLDWQPHDAPGYGWPRGERSW
                     VNPSPNAPNQSMARCYAGTV"
     gene            complement(698..1264)
                     /locus_tag="PKN91_04665"
     CDS             complement(698..1264)
                     /locus_tag="PKN91_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HNS27399.1"
                     /translation="MHKLSCHTDPGDLPIERVEADLTVARGTAAGGARLDVHFRIVAD
                     TGQLCLPALRQGRRTDGLWRHSCFELFVRAPGAAAYAEVNLAPSGDWAAYRFSAYRDG
                     LQPIVPMTAPAIDAVAAADMFELRASLRLDELRPGARQPGTTLLEVALAAVIEECSGR
                     LSYWAANHPDPKRPDFHHPGSFVHEVRY"
     gene            1324..2337
                     /locus_tag="PKN91_04670"
     CDS             1324..2337
                     /locus_tag="PKN91_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HNS27400.1"
                     /translation="MAAKRKSPARKSAALKPRARGKLAPARTRRGLAATEVMIALDSP
                     DIASLAAEVTAAGGAPIGAYRDPLGGRALLLASLPLAAVQPTPFQRDLSPTHTKRLAA
                     KIDEASAFLDPLIVVRGAKGGFWTPNGRHRLAAAKVLGLRQITALVSPDESLAYRILA
                     LNTEKAHNLKDKSLEVIRMARALARDDPSRHEAAYAAQFEAAELLTLGIVYEKNARFG
                     GGAYSPFLKKVDRFAARPLATSLRDRQAWAERLTGIEADVKRIVGALQARGLKSPYLR
                     NYVIARINPVRFHKATKGETGPAMPIAQALTRMAAAARRFDVTKVSSADLAWVAAGAE
                     SAD"
     gene            2394..3392
                     /locus_tag="PKN91_04675"
     CDS             2394..3392
                     /locus_tag="PKN91_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNS27401.1"
                     /translation="MRRAIYALIALLALAGAGYAAFRALQPPRVATATPTRGPAIEAV
                     YATGVVEPGLEIRIAPRVGGRIVELPVDEGAQVRKGQLLARLEAADLVASVAELAARA
                     QYARSQHERNAALRKNALVSADALEQSRAELEAAEAALKRAREQVDHMRLVAPSDGEI
                     TRRDGEVGEFIPVNQVIFYMAGPAPLRITADVDEEDLPRLRPGLPVLIRTDAYPDRVF
                     DGRVDQITPRGDSTARSFRVRIALEGDPPLRIGMTTETNIVLEQRDNALLVPSSAVVD
                     GAVWTIDAGRAARLPVEVGVVGVLQTEIRGGIEGDERLIVAPPDGVSPGDRFRTAD"
     gene            3409..4653
                     /locus_tag="PKN91_04680"
     CDS             3409..4653
                     /locus_tag="PKN91_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS27402.1"
                     /translation="MQLYVDIAWSHLRTRRRQTIASLLGVVLGVAFFLAVSSLMRGSE
                     LDFIARLVDSTPHITVSDEFRDPTVQPAVLRYPAGAVSVLHVKPLTERRGIRQYKRRL
                     EQIDAIPGLRAAPVLAGQVIFSFAGRDEAITLSGIEPARMQDVTTIEDDIVEGSLEAI
                     DADPNGIIIGRALARKLSLAMGDTVSVVSPVGNVRVMRIVGLFHTGRAVYDETQGFAQ
                     LTRVQALLNRPNVANTIVTRLEDPYQARATAAHIERLTGYKSVSWQEASEDILRTLMV
                     RNVIMFTVVGAILLVASFGIYNVISSVIMEKTRDIAILKSMGFRAGDIELVFLIEGLV
                     LGAAGSVLGFLAGSGLIWVTGQVRLAVPGESDPVNLPVYWGWGQFALAMLFAMLSALA
                     ATYLPARRGARLAPVAILRGAA"
     gene            4650..5321
                     /locus_tag="PKN91_04685"
     CDS             4650..5321
                     /locus_tag="PKN91_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS27403.1"
                     /translation="MSAILVAENLTRTLPGEVPVTLVRGISLTIDRGEFVVIKGPSGS
                     GKSSLLYLLGLLDQPTSGALRLEGEDTTAYDEDQLAALRLAKLGFVFQFHFLLAEFSA
                     FDNVRLPMRRLGRLPGHRQRERAMQLLQDLGLAGHEHKHPSQLSGGQRQRVAIARALA
                     NDPPIILTDEPTGALDSKSSINVKEILKDLVRTHDRTVIAVTHDDHFAAGADRQLEII
                     DGRLV"
     gene            5329..6225
                     /locus_tag="PKN91_04690"
     CDS             5329..6225
                     /locus_tag="PKN91_04690"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNS27404.1"
                     /translation="MTGIPLLVTCLAVGVVAARVLAQPARLARGISWWVLRIALPALV
                     LTLLPRLEFERSLWFLIAAMWLVFVGGWALAAVAGARLGWSRARIGAVALTAGLGNTA
                     FTGYPLIEALRGSQALPYAAVADQLGCFIALSTGGVILASWYARERALPGALIRHVVL
                     FPPFLACIAGFIAGQLGGWPAWLDQLLRIIGATLSPLALLAIGLQLRWRIDATQLRAV
                     SLGLGWKLLAAPLLVWLLGIAAGAEGLLLTVGVLQAAMGPMVSATILAEERGLDAAVG
                     HAMLAAGVIASLVSVPLIDTLL"
     gene            complement(6245..7663)
                     /locus_tag="PKN91_04695"
     CDS             complement(6245..7663)
                     /locus_tag="PKN91_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNS27405.1"
                     /translation="MVLLILATGPLHGADLPPRDRDSLLRAVDARAARAGATALEIWN
                     WAELGYQEERSSALLQKELADAGFSVQSGVAGMPTSFIATAGAGEPVIAILAEFDALP
                     GISQAAVAERAAIDGKNAAHACGHNLFGAGSASAAIVLKNWLAARKLPGTIRLYGTPA
                     EEGGAGKAYMVRAGLFDDVDVALHWHAGDRNDASQNGSNSNKSGKFRFHGISSHAAGA
                     PDKGRSALDGVEAMNIMANMMHEHMPMEARLHYVITRGGAAPNVVPDFAEVFYYVRHP
                     DPRLVQQLWERLTKIAEGAALGTGTTWDVEVIHGAYPLLPNHTLGRAMQASLERVGGV
                     HYDADETAFAQRIHATLDNPRRALGSERLVQPYAPEQHFGSTDVGDVSWSVPTVGLNT
                     ATWVPGTPAHSWQAAAAAGMSIGMKGMHNASKALVLTAARLYLDPALVAAARREFVEA
                     RGADFVYRPMIGERAPPLDYRR"
     gene            7716..8975
                     /locus_tag="PKN91_04700"
     CDS             7716..8975
                     /locus_tag="PKN91_04700"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNS27406.1"
                     /translation="MVAHRGIGTTAWLALLLAAPWAPADAGDDALARANRVLSHTILV
                     DGHNDLPIAIRGHASAPGDLDAYDLRGTVPGQTDIPRLRAGHVGAQFWSVYIPGELQN
                     GQARMQLEQIDIARRLIAKYPDVFQLVGTAAEVRAAHRAGRIGSLLGMEGGYGLENSF
                     APLRAYYDLGVRYMTLTHNSHTDWADSAMPETPKFGGLSPFGEQVVREMNRLGMLVDL
                     SHTSADTMRDTLDVSKAPVIFSHAAARGLVDISRNVPDDVIARLPANGGVLMVTFVVG
                     FVSADAANVLFPAMKEFYTSSAQAASAGERRALYEEFKKNLVLPRVTVGDVADHIEYV
                     VSLAGIDHVGIGGDFDGNDLWPEGLSDVSMYPNLFAELIRRGWSDRDLAKLAGGNVLR
                     AMEQAERVARELQRVTPAANSISRAGT"
     gene            complement(8953..9573)
                     /gene="alkB"
                     /locus_tag="PKN91_04705"
     CDS             complement(8953..9573)
                     /gene="alkB"
                     /locus_tag="PKN91_04705"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: protein motif:HMM:NF011930.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HNS27407.1"
                     /translation="MNQPAAIAIAREVTLLRGFAAPTGPLIDAIALVAARAPFRRLRT
                     PGGGTMSVAMTNCGPWGWHSDARGYRYVGRDPLTGDPWYPMPEPFFRLAGQAAARAGF
                     PGFEPDACLVNRYEPGASLGAHRDHDELDMRFPIVSVSIGLPALFLWYGERRGGTPIA
                     TLVEDGDVVVWGGSARAGYHGVRKLQAGEHPATGALRYNLTFRRAK"
     gene            9617..10858
                     /locus_tag="PKN91_04710"
     CDS             9617..10858
                     /locus_tag="PKN91_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HNS27408.1"
                     /translation="MSPTNRIILSLVLGLGLGAAIAALGSSDLARIVTALQPIGTLWV
                     NALRMTVMPLVFSVLVTGVASAASAANAGKLAARTLATFMVFLVGAVMAAAVLAPLIL
                     DTFQIPSSAAQALRAGALGLAQPVPDIAPVDEWLIGIVPVNPVQAAAEGAVLPLIVFA
                     LFFAFATTRIAPDSRDRITGFFQAVADAMITIVKWILWAAPVGVFALALGVGYHGGLN
                     AAGALLYYVVLLSVMCLLATGAMYPIAALSKDITLPQFARGAAAAQVVAASTQSSLAT
                     LPAMIEVAQKEFHLPAGVVGLVLPLAVSLFRVSTAIANLSAAIFVAAIYGIDLTPVQL
                     ATGALVSIATSIGSVGVSSQVSYFFGVMPICLAMGLPVEVLALLLAVEVLPDVFRTIC
                     NVTADLTATVLVGRERRATPR"
     gene            10842..11567
                     /locus_tag="PKN91_04715"
     CDS             10842..11567
                     /locus_tag="PKN91_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNS27409.1"
                     /translation="MQPPADPRAARGRSGLAAALSALLLLAGLLLAFVELADEVIDGD
                     THAFDRAVLLALRTSSDSADPVGPAWLETAFRDITTLGGTPVLTTVTLVTLGYLLLAG
                     RRATALLVTVAVGGGVLLSNLLKAGFERPRPDLVAHLVDISSLSFPSGHAMMSTVTWL
                     TLGALLATVQSTRRMKAYVLAVGVALALLVGVSRVYLGVHWPTDVLADWCIGAAWALG
                     CWLFAGWLQRCRQHRRSGIIAGA"
     gene            complement(11564..12829)
                     /locus_tag="PKN91_04720"
     CDS             complement(11564..12829)
                     /locus_tag="PKN91_04720"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS27410.1"
                     /translation="MESRERRSDRGAAQLSRYRHLTSAAWRQLWRYRTRSLLVMACAA
                     LGVTGSIAAVNYASGGRQQVIDQVARLGTNVIVVAALQDRSTGGRARTGNIATTLRED
                     DYRSLRREVPAIVRSSALASASLRLKAAGLSKVAPVVGCEPDFFRIKHWAVAEGEVFD
                     DAQLRRSARVVLLGATIAKDLYDGQSPLGERLFINRVPFEVIGVLAERGQGLDATNED
                     AQVYVPLTAAMRRLLNVEHYNALVLEVGSFPAMAGAVRDITDLLRDRHRTGSFRPDDF
                     SVLNQQELGDTRVAAAERLGFFVRWIGLSALLVAGVGVLAIAWITVRERTREIGTRRA
                     LGATARDIFFQFAFEAMVLATAGAVCGLAAGWLVSRLVAARTALPFVFDGGNAALALA
                     AAILINLAFVCWPARRAARLDPIVALRHA"
     gene            complement(12784..14007)
                     /locus_tag="PKN91_04725"
     CDS             complement(12784..14007)
                     /locus_tag="PKN91_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS27411.1"
                     /translation="MSGLRVIGAAWRSLTRSKLRTFFMMLGVAVGVAALTALASVGEA
                     TRQETLRQFKRMLGTFDTLTIRPGAGSTRGMPSVSTVEPSLKFADGPAIAEGVPGVLR
                     VAQVQQTFDLDVSYRDESVVPLVLGVSSNWADIRDEAVASGSHLTEEDERALARVAVI
                     GADVASVLFKGEDPLGKVIRIADVPFQVKGVMATRGAGPGGASLDNMLVIPVTTASKR
                     LFNRDYLSTLVVQLRNEGDSDRVADDVRALLRERHRIIPPAEDDFTVSSPRATLAQIQ
                     QVGSTLSKIVTGVAAIATLIGGAVIMSLMLIGVSERRREIGVRRAVGATRADILAQFL
                     AEATLISALGGAVGIVIGAGGATVGALLQQLPPAYMWSMIGGAVALSLAVGIVFGLYP
                     AWKAANVDPIEALRS"
     gene            complement(14004..14693)
                     /locus_tag="PKN91_04730"
     CDS             complement(14004..14693)
                     /locus_tag="PKN91_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS27412.1"
                     /translation="MIEASHLSRTYTRGDGTPVTALRDVSLAIAPGEFVVIRGASGSG
                     KSSLLNILGCLDTPTSGSYRLGGENVSALSDNERSHVRSKRIGFVFQNFNLLPRATAL
                     ENVELPTLYTGAAPDHARARQLLAQVGLDGRADHFATELSGGEQQRVAIARALMNDPP
                     LLLADEPTGNLDSAAGESVMQLLTSLQREGRTIVLVTHDEHVASFAEREIVIRDGVVA
                     SDTRRRERVQA"
     gene            complement(14697..15764)
                     /locus_tag="PKN91_04735"
     CDS             complement(14697..15764)
                     /locus_tag="PKN91_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNS27413.1"
                     /translation="MRRAAALAATGAVLAVLVAGVYWWQARPGEGPADAAVRVVTPER
                     RTVASSVLATGVVRLRVGAEVRVGSQVSGIVRELNVTVGSHIDRGAVIARIDSRGIEA
                     RLAQARAQIDVIEQDVARARVELARVQRLEEQRLVAESEVEDRELALAEATARLEKAR
                     RDAAVVETELGYAVIRAPIAGTVASVTTQEGETVAASFTTPTFVTIIGDDALQLVAMV
                     DETDIGGVAPGNPVGFTVEAFPAEEFRGHVERVAPKGTIISGVVNYEVMIGIDGSAEL
                     LKPDMTANVTIETAQREALVVPNEAVLREEGTRYVLVQTEAGAERREVVVGARAGGVT
                     EIRRGLAPTERVLVGAVRQEN"
     gene            complement(15761..16255)
                     /locus_tag="PKN91_04740"
     CDS             complement(15761..16255)
                     /locus_tag="PKN91_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27414.1"
                     /translation="MQGRTEMNRVATCLLSAILCGALPAFAADAPTNFAGTWKLDTAR
                     GENLGMMAAMQQTLVITQSSAQLVLREASDFQGQKSEREVRYDLSGAPVTNPGPMGGA
                     TETVAQWVGDKLVVTSTAEGSVAGTKTVRTETRSLSSDGRTMTVESVRGAGKPVVMVY
                     GKVE"
     gene            16409..17515
                     /locus_tag="PKN91_04745"
     CDS             16409..17515
                     /locus_tag="PKN91_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27415.1"
                     /translation="MRMRIPAPLPYRLVLLAVALAAGLVSAVEPAGPAPAPADARELP
                     SHMIPNIPDAAEAYWAPDSRHLIAQTRDRRAVPMPNGSPGALTWIFTDDGTEMWNVND
                     RGQDGCSFFFPDSQRIVWTSTRDRMDLPVGNWSDSDNYPRGAELYSSDLHGGHLQRLT
                     NNEWYEAEVSVSPDGKWITFGRQIDGNMDIWVMRSDGTGKFQVTRTGDWQEGAPFFMP
                     DSEHIIFRAWRRADYKNVRPTPMTIFTIRRDGTDWRRHTYDDGMNWHPVPAPDGRHYV
                     YVKALTETNWEIYLGDLAGGEHRRLTYFDGLDILAHVSPDGRKMNWARGAGAGFTQGF
                     RTHVMDVSSLGIGPERAVPFDPSWGRPMTAGTSD"
     gene            complement(17544..18710)
                     /locus_tag="PKN91_04750"
     CDS             complement(17544..18710)
                     /locus_tag="PKN91_04750"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNS27416.1"
                     /translation="MSRTGRQPVVISASLARRIWLRAQRLDSCAPFGDGPGATRAAVE
                     HLGYVQIDTIHVVERCHHQILYTRIPAYRREHLHQAQAIDKSVFECWTHALAYVPTRD
                     LHVHLGAMRRNWQANIGWFGSVTRTDLRRVLARIRAEGALSIRDIDDDDLVDRHHPWA
                     SRKPSKRALECAFFKGVVTVSERRGMVKTYELIGRHFGWDAPPRLASEREIVAHRLDC
                     ALRAQGIVSLDSICHLDARRKPAVRRLIESTVRSGELLAVVVEGAEKVPHWASPQALE
                     ARVQAPGEQVHILSPFDPLVIQRRRLQLLFGYQHRFEAYVPKAKRQFGYFAQPVLVGD
                     EIVAALDLKTDRARGRLLVQQWTWLGRQPRRALKPRIEEALHRFERFQLPGHWP"
     gene            complement(18731..19309)
                     /locus_tag="PKN91_04755"
     CDS             complement(18731..19309)
                     /locus_tag="PKN91_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNS27417.1"
                     /translation="MPTRPDTPLARLSREQLPEHMRGAWDQASQLHGDTTFIEVFGQA
                     PEVYDWYLEEFYRKLFYSGRIERSVVELVRLRLANVHGCAFCNRSDTLAALAGGVPQA
                     KIDALAGYEDGPFTDREKAALALADVMVLTNARGHITTALYQRLRAHYSDGEIVELGV
                     IMAVLCGMAKMVFAFDLVEKMDSCPWLPPGEG"
     gene            complement(19336..20253)
                     /locus_tag="PKN91_04760"
     CDS             complement(19336..20253)
                     /locus_tag="PKN91_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNS27418.1"
                     /translation="MTPASPRRAAPGVLLALALAALAPAAAAELHHSFVPATDGVPLA
                     VTECGTVAAPAVLLLHGFGQSHLSFSEQFTPQACAQLHLVAFDLRGHGGSGKPWAPEA
                     YSERERWADDVRSVMTARQLDRPLLVGWSFGGNVALHYIAAHGADSLRGLLLVGSHGG
                     LLASATSRGGIRSERMAQLSAAAMTPDIPGQIAAAEAFARMLTAKPLPDALARQLLVA
                     NLMLPAYARAAMRGLPVDNSGLEGKLTLPVRIVVGAHDASMDIAALQALAARLPQASL
                     VVYDDSGHSPFLEEAGRFNRELAGFSGAR"
     gene            complement(20250..21698)
                     /locus_tag="PKN91_04765"
     CDS             complement(20250..21698)
                     /locus_tag="PKN91_04765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNS27419.1"
                     /translation="MTTNRREFIAGGAAALTGAALYRSVAYAQAQSVADVIVIGAGLS
                     GLGAAGLLEEAGLRVTVLEGRQRIGGRVYTRYDLPGRPELGGSSATPGYGRWIAAAEK
                     CGVGLTDLRATTTRSPGQVLVLDGKIISPEDWPRSPHNVFPEVDKNTMPWAYGGKLFA
                     PLNPLKSFSDWILPSHRADDISLNDFLLAKGVDQRVIDLAFSTVPSYGDTAHDVSVLM
                     FAFIASWGRASSAVSTKRASYRITGGNQRLPEAMARMLKGDVRLGARVVGIEANADSG
                     VVTLESGERIRARAIISSMPYSTLRHVRIAPGLNGVQHEAVHRLAYQNLTQVVLIPRT
                     PFWLEDGLPPSMWTDGPAGWISAEGVGRSDDGRGYFMVYGRGRTGLYWDRLGQKAAGE
                     LIIREIERLRPAAKGQLEVGAVHSWTMDPWNAGDWAVFGPGQVTRYLPAMAAPHQRLF
                     FCGEHTSLANRGMEAALESAERAAIEVLGELR"
     gene            complement(21822..>22174)
                     /locus_tag="PKN91_04770"
     CDS             complement(21822..>22174)
                     /locus_tag="PKN91_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS27420.1"
                     /translation="ETFALSATWTDTLNSDWEWYLRGDASYQGKAYLDETNLAWVGAY
                     SLLNGRIGFAREDGLNVELFCRNCLNEEGWRTGRRAVDWVDTAPLPNNLTQVGALVQP
                     FEKREFGIRVRFEY"
BASE COUNT         3423 a         7889 c         7438 g         3424 t
ORIGIN      
        1 gaccgtgccg gcatagcagc gcgccatcga ctggttcggt gcgttcgggc tcggattgac
       61 ccagctgcgc tcaccgcgcg gccagccgta gccgggcgcg tcgtgtggct gccagtcgag
      121 catcgtcacc acttcgtagt cgacctcgag acccagcgtc gcgacgaacc agtgcgcaag
      181 ttcgcccatc gtcagtccat ggcgcatcgg cagcggaccc gcaccgacga agctctccca
      241 tcccgcgcgc agcactgtgc cttcgatcgg gcggcccaca gggttcggcc gatcgaggat
      301 ccagacggcc ttgtcatgct ctgccgccgc ctcgagcaca tagcgcagcg tggtgatgaa
      361 ggtgtagatg cggcagccaa ggtcctgcag gtcgaccagc agcacatcga atccggccat
      421 catctccggg gtcgggcgtc gcacttcgcc atagaggctg aagacgggaa tcccggatgc
      481 cggatcggcg aaatccgtcg actcgaccat gttgtcctgc ttgtcgccgc gcaggccgtg
      541 ctgcgggccg aatgccgcgg tgagctcgat gtcgggcacg gcggcaagcg catcgagcga
      601 atggccgaga tccggcagga ccgacgccgg atgggcgagc agcgccgcgc gccggcccgc
      661 caggccgcgg cgcaggcggg gctcgccgat cagtcgctca ataccgcact tcatgcacga
      721 aactcccggg atgatggaaa tcgggtcgct ttggatcggg atggttcgct gcccagtatg
      781 acagcctgcc gctgcactcc tcgatcacag ccgccagagc aacttcgagc aatgtcgtac
      841 ctggctggcg cgcgcctggc cgcaactcgt cgagacgcag agacgcgcgc agctcgaaca
      901 tgtccgccgc ggcaaccgcg tcgatcgccg gcgcagtcat cggcacgatc ggttgcagac
      961 catcgcgata ggcgctgaag cggtaggccg cccagtcacc cgacggcgcg aggttcacct
     1021 cggcgtaggc ggcggcgccg ggcgcgcgca cgaacagttc gaagcagctg tgacgccaga
     1081 ggccgtccgt acgccggccc tgccgcaacg caggcagaca caactgtccg gtgtccgcca
     1141 cgatccggaa gtgcacgtcg agccgcgcgc cacccgccgc ggtgccgcgc gccacggtca
     1201 ggtctgcttc gacgcgctcg atcggcagat cgccgggatc ggtatggcag gaaagcttgt
     1261 gcacgccgtg cgatgttaca ttttccggtg gccgcgcagc cgcgcgccgt gcacactcac
     1321 cccgtggccg ccaagcgcaa gagtcccgcc cgcaagagcg ccgccctgaa gccgcgcgca
     1381 cgcggcaaac tcgcgcccgc gcgcacccgc cgcggtctcg ccgcgaccga ggtcatgatc
     1441 gcgctcgact cgcccgacat cgcgtcactc gccgccgagg tcaccgctgc cggcggcgca
     1501 ccgatcggag cctatcgcga cccgcttggc ggacgtgcac tgctgctcgc gtcgctgccg
     1561 ctcgctgcag tccagccgac gcccttccag cgcgacctgt cgccgaccca tacgaagcgc
     1621 ctcgcggcca agatcgacga agcgtccgcg ttcctcgatc cgctgatcgt cgtgcgcggc
     1681 gccaagggcg gcttctggac accgaacggg cgccaccgcc tcgccgcggc gaaggtgctc
     1741 ggcctgcgcc agatcactgc gctggtttcg cccgacgagt cgctcgccta ccgcatcctc
     1801 gcgctcaaca ccgagaaggc gcacaacctg aaggacaaga gcctcgaggt gatccgcatg
     1861 gcgcgcgcgc tcgccaggga cgatccgtca cggcacgagg cggcgtacgc ggcgcagttc
     1921 gaagccgccg agctgctgac gctcggcatc gtctacgaga aaaacgcccg tttcggcggc
     1981 ggcgcctaca gcccgttcct gaaaaaagtc gatcgcttcg ccgcacgccc gctcgcgacc
     2041 agcctgcgcg accggcaggc ctgggccgag cggctcaccg gcatcgaggc cgacgtcaag
     2101 cgcatcgtcg gcgcgctgca ggcccgcggc ctgaaatcac cctacctgcg caactacgtg
     2161 atcgcacgta tcaacccggt gcgcttccac aaggcgacga agggcgagac gggccccgcg
     2221 atgccgattg cccaggccct gacgcgcatg gcggccgcgg ccaggcgctt cgacgtcacg
     2281 aaagtctcga gcgccgatct cgcctgggtg gccgcgggcg ccgaatccgc ggattgaccg
     2341 gtcgcaaggc gctccgcagc cgcccgcccg ggctgattgc tacactgcgc cggatgcgtc
     2401 gcgccatcta tgccctgatc gcactcctgg cacttgccgg cgccggctat gccgcttttc
     2461 gcgcactgca gccaccgcgg gtggcgaccg ccacaccgac ccgtggcccg gcgatcgagg
     2521 cggtgtacgc caccggcgtc gtcgagcccg gcctcgagat ccgcattgcg ccccgtgtgg
     2581 gcgggcgcat cgtcgagttg ccggtcgatg agggtgcgca ggtgcgcaag ggacagctgc
     2641 tcgcgcggct cgaggccgcg gatctcgtcg catccgtcgc ggaactcgcc gcacgcgcgc
     2701 aatatgcgcg ctcgcagcac gagcgcaacg cggcgctgcg caagaacgcg ctcgtatcgg
     2761 ccgatgcgct cgaacagtcg cgcgccgaac tcgaagccgc cgaggcggca ctgaagcgcg
     2821 cacgcgagca ggtcgaccac atgcgcctcg ttgcgccgag cgacggggag atcacgcgcc
     2881 gcgacggcga agtgggtgaa ttcatccccg tgaaccaggt catcttctac atggcgggtc
     2941 ctgcgccgct gcgcatcacg gccgacgtcg acgaggaaga cctgccgcgc ctgcggccgg
     3001 ggctgccggt actgatccgc accgacgcct acccggatcg ggtgttcgac gggcgcgtcg
     3061 accagatcac gccacgcggc gactcgaccg cgcggagttt ccgggtgcgc atcgcgctcg
     3121 agggcgaccc gccgctgcgc atcggcatga ccaccgagac gaacatcgtg ctcgagcaac
     3181 gcgacaatgc gctgctcgtg ccctcctccg cggtggtgga tggtgccgtg tggacgatcg
     3241 atgcgggtcg cgcggcgcgc ctgccggtgg aagtcggcgt cgtcggcgtg ctgcagaccg
     3301 agatccgcgg cgggattgag ggtgacgagc ggttgatcgt cgccccaccc gacggcgtct
     3361 cgcccggcga ccggttccgg accgcggact gacgcgcccg ccgcgcccgt gcagctgtac
     3421 gtcgacattg cctggagcca cctgcgtacc cggcgcaggc agaccatcgc ctccctgctc
     3481 ggcgtcgtcc tcggcgtcgc attcttcctc gccgtgtcgt cgctgatgcg cggctccgaa
     3541 ctcgacttca tcgcgcgcct cgtcgacagc acgccgcaca tcacggtatc ggacgaattc
     3601 cgcgacccga ccgtccaacc cgctgtgctg cgctatccgg cgggcgccgt cagcgtgctg
     3661 cacgtcaagc cgctcaccga gcggcgcggc atccgccagt acaaacgtcg gctcgaacag
     3721 atcgacgcga ttcccggcct gcgcgccgca ccggtgctcg ccggccaggt gatcttcagc
     3781 ttcgctggcc gcgacgaggc gatcactctt tccggcatcg agccggcccg catgcaggat
     3841 gtcaccacga tcgaggacga catcgtcgag ggctctctcg aggcgatcga tgcggacccg
     3901 aacggcatca tcatcggccg ggcgctcgcg cggaagctct cgctcgcgat gggcgacacc
     3961 gtctcggtgg tctcgccggt cggcaacgtg cgggtgatga ggatcgtggg cctgttccac
     4021 accggccgcg cggtctatga cgaaacacag ggttttgcgc aattgacccg cgtgcaggcg
     4081 ctgctgaatc ggccgaacgt cgccaacacg atcgtcaccc gcctcgagga tccctaccag
     4141 gcacgcgcga cggcggcgca catcgagcgc ctgaccggct acaagtcggt gtcgtggcag
     4201 gaggcgagcg aagacatcct gcggacactg atggtgcgca acgtgatcat gttcaccgtg
     4261 gtcggcgcga tcctgctggt cgcctcgttc ggcatctaca acgtgatctc gagcgtgatc
     4321 atggagaaga cccgcgacat cgcgatcctg aaatcgatgg gcttccgcgc cggcgacatc
     4381 gaactggtct tcctgatcga ggggctcgtg ctcggtgcag ccggcagcgt actcggcttc
     4441 ctcgccggca gcgggctgat ctgggtgacc ggacaggtgc gcctggccgt tccgggcgaa
     4501 tcggacccgg tcaaccttcc cgtctactgg ggctggggcc agttcgcgct ggcgatgctg
     4561 ttcgcgatgc tgtcggcgct cgccgccacc tacctgcccg cgcgccgcgg cgcacgcctc
     4621 gcgccggtgg cgatcctgcg tggtgcggca tgagcgcgat actggtcgcg gagaatctca
     4681 cgcgcacatt gccgggcgag gtgccggtga cgctggtgcg gggcatctcg ctcaccatcg
     4741 atcgcggcga gttcgtcgtc atcaaggggc catcgggctc gggcaagtcc tcgctgctgt
     4801 acctgctcgg gctgctggac cagccgacct ccggagcact gaggctcgag ggcgaggaca
     4861 ccaccgccta cgacgaggac cagctcgccg ccctgcgcct cgcaaagctc ggcttcgtat
     4921 tccagttcca cttcctgctg gccgagtttt ccgccttcga caacgtgcgc ctgcccatgc
     4981 gccggctcgg ccgcctgccc ggccaccgcc agcgcgagcg ggccatgcag ctgctgcagg
     5041 acctcggact cgccgggcac gagcacaagc acccctcgca gctctccggc ggacagcgcc
     5101 agcgcgtcgc gattgcgcgc gcgctggcga atgacccgcc gatcatcctc accgacgagc
     5161 cgaccggcgc gctcgattcg aaatcgagca tcaacgtgaa ggaaatcctg aaggatctgg
     5221 tgcgcaccca cgatcgcacc gtcatcgccg tcacgcacga cgatcacttc gccgccggcg
     5281 ccgaccggca gctcgagatc atcgacggac gactcgtata gcgcggccat gaccggcatc
     5341 ccgttgctcg tcacctgcct cgccgtcggt gtcgttgctg cccgcgtact cgcgcagccg
     5401 gcgcgcctcg cgcgcggcat cagctggtgg gtgctgcgca tcgcgctgcc ggcgctggtt
     5461 ctgaccctgc tgccgcggct cgagttcgag cgcagcctgt ggttcctgat cgccgcgatg
     5521 tggctcgttt tcgtcggcgg ctgggccttg gccgcggtgg ccggcgcgcg cttaggctgg
     5581 tcgcgcgcgc gcatcggcgc ggtcgcgctg accgccgggc tcggcaatac ggcgttcacg
     5641 ggctatccgt tgatcgaagc cctgcgcggc tcccaggccc tgccgtacgc cgcagtggcc
     5701 gatcagctcg gctgtttcat cgcgttgtcg accggcggcg tgatcctcgc ctcatggtac
     5761 gcccgcgagc gcgcgctgcc gggagcactg atccggcatg tcgtgctgtt tccgccgttc
     5821 ctcgcctgca tcgccggatt catcgccggg cagctcggcg gctggccggc ctggctggac
     5881 cagctgctgc gaatcatcgg cgccacgctc tcgccgctgg cgctgctcgc gatcggcctg
     5941 cagctgcgct ggcgtatcga tgcgacccag ctgcgcgccg tgagcctcgg tctcggctgg
     6001 aagctcctgg ccgcgccgct gctcgtctgg ttgctcggca tcgcggcagg cgcagagggc
     6061 ctgctactca cggtaggcgt acttcaggcc gccatgggtc cgatggtgtc ggcgacgatc
     6121 ctcgccgagg aacgcggact cgacgcggcg gtcgggcacg ccatgctcgc ggccggcgtg
     6181 atcgcctcgc tcgtcagcgt gccgctgatc gacacgctgc tgtagcgggt ctgcccgcgc
     6241 ggattcacct gcggtaatcg agcggcggcg cgcgctcgcc gatcatcggt cgatagacga
     6301 agtcggcgcc gcgcgcctcg acgaattccc ggcgggctgc cgcgacgagc gccgggtcga
     6361 gatacaggcg agccgcggtc agcacgagcg ccttcgatgc attgtgcatg cccttcatgc
     6421 cgatgctcat gccggccgcc gccgcggcct gccagctgtg cgccggcgtg ccgggcaccc
     6481 aggtcgcggt gttgaggccg accgtcggca cgctccagct gacatcgccg acgtcggtcg
     6541 agccgaagtg ctgctcgggc gcgtagggct gcacgagccg ttcgctgccg agtgcgcgcc
     6601 gcggattgtc gagcgtcgcg tggatgcgct gcgcgaaggc cgtctcgtcg gcgtcgtagt
     6661 gcacgccgcc gacgcgctcg aggctcgcct gcatcgcgcg accgagtgtg tggttcggca
     6721 gcagcggata ggcgccgtgg atcacctcga cgtcccaggt cgtgccggtg ccgagcgcag
     6781 cgccctccgc gatcttcgtc agacgctccc acagctgctg caccaagcgc ggatcgggat
     6841 ggcgcacgta gtagaacact tcggcgaagt ccggcacgac attcggcgcc gcgccaccac
     6901 gcgtgatcac gtagtgcagg cgcgcctcca tcggcatatg ctcgtgcatc atgttcgcca
     6961 tgatgttcat cgcctcgacg ccatcgagcg ccgagcggcc cttgtccggc gcgccggccg
     7021 cgtggctcga aattccgtgg aagcggaact tgcccgactt gttgctgttc gagccgttct
     7081 ggctcgcgtc gttgcgatcc ccggcgtgcc agtgcagcgc gacgtccacg tcgtcgaaca
     7141 ggcccgcgcg caccatgtag gccttgccgg caccgccctc ctcggccggc gtgccataga
     7201 ggcggatcgt gcctggaagc ttgcgcgcgg cgagccagtt cttcagcacg atcgcggcgc
     7261 tcgccgaacc tgcgccgaag agattgtggc cgcaggcatg cgccgcgttc ttgccgtcga
     7321 tcgccgcgcg ctcggccacc gccgcctgcg aaatgccggg cagcgcgtcg aactccgcga
     7381 ggatcgcgat caccggttcg ccggctccgg cggtcgcgat gaagctggtc ggcatgccgg
     7441 cgacaccgga ctgcacgctg aagcccgcat cggcaagctc cttctgcaga agcgccgagc
     7501 tgcgctcctc ctggtagcca agctcggccc agttccagat ctcgagggcg gtcgcgccgg
     7561 cccgtgctgc ccgcgcatcg actgcgcgca gcaacgaatc gcggtcgcgg ggagggaggt
     7621 cggcgccgtg caacggaccg gtggccagaa tgaggaggac catggcgcca atacgcatta
     7681 gagtcatgat tcgagctccg caggaggatt gccgcatggt agcgcatcgg ggaatcggga
     7741 cgaccgcatg gctcgccctg ttgttggcag cgccgtgggc gccggcggac gcgggcgacg
     7801 atgcgctggc gcgcgccaat cgggtgctgt cgcataccat tctcgtcgac ggccacaacg
     7861 acctgccgat cgccatccgg ggccacgcca gcgcaccggg cgacctcgac gcctacgacc
     7921 tgcgcggcac cgtgcccggg cagaccgaca tcccgcgcct gcgcgcaggt cacgtcggcg
     7981 cacagttctg gtcggtgtac atcccggggg aactgcagaa cgggcaggcg cgcatgcagc
     8041 tcgagcagat cgacatcgcg cgacgcctga tcgcgaagta cccggacgtg ttccagctcg
     8101 tcggtacggc agcggaggtg cgcgccgcgc accgcgccgg gcgcatcggc tcgctgctcg
     8161 gcatggaggg cggctacggc ctcgagaact cctttgcccc gctgcgcgcc tattacgacc
     8221 ttggcgtgcg ttacatgacc ctgacgcaca actcgcacac cgactgggcc gattcggcga
     8281 tgcccgaaac gccgaagttc ggcggcctgt cgcccttcgg cgagcaggtg gtccgcgaga
     8341 tgaaccgcct cggcatgctc gtcgacctgt cgcacacctc ggcggacacg atgcgcgaca
     8401 cgctggacgt gtcgaaggca ccggtgatct tctcgcacgc cgcggcgcgc ggcctcgtcg
     8461 atatctcgcg caacgtaccg gacgacgtga tcgcgcgcct gccggccaat ggaggcgtgc
     8521 tgatggtcac ctttgtcgtc ggcttcgttt cggccgacgc ggccaacgtg ctgtttccgg
     8581 ctatgaagga gttctacacg agttcggcgc aggccgcttc cgccggggaa cgccgcgcgc
     8641 tgtacgagga attcaagaag aacctggtgc tgccgcgcgt cacggtcggc gacgtcgccg
     8701 accatatcga gtacgtcgtc agcctcgcgg gtatcgacca tgtgggcatc ggcggtgact
     8761 tcgacggcaa cgacctgtgg cccgagggcc tgagcgacgt ctcgatgtac ccgaacctgt
     8821 tcgccgagct gatccgtcgc ggctggtcgg atcgcgacct cgccaagctc gccggcggga
     8881 atgtgctgcg cgcgatggag caggccgagc gtgtggcgcg cgagctgcag cgggtcactc
     8941 ccgcggcgaa ttctatttcg cgcgccggaa cgtgaggttg tagcgcaacg cacccgtggc
     9001 cgggtgctcg ccggcctgga gcttgcgcac gccgtggtaa cccgcgcgtg cgctgccgcc
     9061 ccaaacgacc acgtccccgt cctcgacgag cgtcgcgatc ggcgtgccgc cgcgacgctc
     9121 gccgtaccag aggaacagcg ccggcagccc gatcgacacc gaaacgatcg ggaagcgcat
     9181 gtcgagttca tcgtggtcgc ggtgagcgcc gaggctcgcg cccggctcgt agcggttgac
     9241 gaggcaggcg tcgggctcga atccggggaa gccggcgcgc gccgccgcct ggcccgcgag
     9301 ccggaaaaac ggctccggca tcggatacca gggatcgccg gtgagcggat cgcgcccgac
     9361 gtagcgatag ccgcgcgcgt cgctgtgcca gccccacggg ccgcaattgg tcatcgcgac
     9421 cgacatggtg ccgccgccgg gcgtcctcag gcggcgaaac ggcgcgcgcg ccgcgaccag
     9481 cgctatcgca tcgatgagcg gcccggtcgg cgcggcaaag ccgcgcagca gcgtcacctc
     9541 gcgcgcgatg gcgattgcgg ccggctgatt catgcgacac tctagccacg aacaggcgcc
     9601 acaagaaagc ccgcgcatgt caccgaccaa ccgcatcatc ctgagcctcg tgctgggcct
     9661 cggtctcggc gcggccatcg cggcgctcgg cagcagcgac ctcgcgcgca tcgtcacggc
     9721 cttgcagccc atcggcacgc tgtgggtgaa tgcgctgcgc atgaccgtga tgccgctggt
     9781 gttctcggta ctggtcacgg gggtcgcatc ggcggcgagc gccgcgaacg ccggcaagct
     9841 cgccgcgcgc acgctcgcga cgttcatggt gtttctcgtc ggtgccgtca tggccgctgc
     9901 cgtcctcgcg ccgctgatac tggacacctt ccagattccc tcgagcgcgg cgcaagcgct
     9961 gcgcgcaggc gccctgggcc ttgcgcagcc ggtgccggac atcgcgcccg tcgacgaatg
    10021 gctcatcggc atcgtgccgg tcaacccggt gcaggcggcc gccgagggcg cggtgctgcc
    10081 gctcatcgtg ttcgccctct tcttcgcctt cgccacgaca cgcatcgcgc ccgattcccg
    10141 cgatcgcatc accgggttct tccaggccgt cgcagacgcg atgatcacga tcgtgaagtg
    10201 gatcctgtgg gcggcgccgg tcggcgtgtt cgcactggcc ctgggcgtcg gttaccacgg
    10261 cggcctgaac gcggccggcg cactccttta ttacgtggtg ctgctctcgg tgatgtgcct
    10321 gctcgcgacc ggtgccatgt atccgatcgc ggcactcagc aaggacatca ccctgcccca
    10381 gttcgcgcgc ggcgcggccg ccgcgcaggt cgtggccgcg agcacgcaat cctcgctggc
    10441 aacgctgccg gcgatgatcg aagtggcgca gaaggagttc catctgcccg cgggcgtcgt
    10501 ggggctcgta ctgccgctcg ccgtgtcgct gtttcgcgtg agcaccgcca tcgccaacct
    10561 cagcgccgcc atcttcgtcg ctgcgatcta cggcatcgac ctcacgccgg tacaactcgc
    10621 gaccggggcg ctcgtgtcga tcgcgaccag catcggcagt gtcggcgtat caagccaggt
    10681 gagctatttc ttcggcgtga tgccgatctg cctggcgatg gggctgccgg tcgaggtgct
    10741 cgcgctgctg ctggcggtcg aggtcctgcc ggatgtcttc cggacgatct gcaatgtcac
    10801 cgccgacctc accgccacgg tgctggtggg tcgcgagcgt cgtgcaaccc cccgctgacc
    10861 cgcgcgccgc acgcggccgc tccggactcg ccgcggccct gtcggcactg ctcctgctcg
    10921 cgggactgct gctcgcgttc gtggaactcg ccgacgaggt catcgacggc gacacgcacg
    10981 ccttcgatcg cgccgtgctg ctcgcactgc gcacgagcag cgacagcgcc gatccggtcg
    11041 gcccggcctg gctcgaaacc gcgttccgtg acatcacgac cctgggcggt acgcccgtac
    11101 tcaccaccgt tacgctggtc acgctcggct acctgctgct cgcgggccgg cgcgcgaccg
    11161 cgctgctcgt cacggtcgca gtcggtggcg gcgtgctgct gtcgaacctg ctgaaggcgg
    11221 gctttgagcg accccggccc gatctcgtcg cgcatctcgt cgacatcagc tcgctcagct
    11281 tcccgagcgg acacgcgatg atgtcgaccg tcacctggct cacgctcggc gcattactcg
    11341 ccaccgtaca gtcgacgcga cgcatgaagg cctacgtgct cgcggtcggc gtcgcgctcg
    11401 cattgctggt cggcgtgagc cgcgtatacc tcggcgtgca ctggccgacc gacgtgctcg
    11461 ccgactggtg tatcggcgcg gcctgggcgc tcggctgctg gctcttcgcg ggctggctgc
    11521 agcggtgccg gcagcatcgc cggtccggca tcattgccgg cgcctaagcg tgcctcagcg
    11581 caacgatcgg atcgagccgc gccgcgcgcc tcgcgggcca gcagacgaac gcgagattga
    11641 tcaggatggc tgcagccagc gccagcgcgg cgttgccgcc gtcgaacacg aacggcagcg
    11701 ccgtgcgcgc cgcgacgagt ctcgacacca gccaacccgc ggcaagaccg cagaccgcgc
    11761 cggcagtggc aagcaccatc gcctcgaagg cgaactggaa gaaaatatct ctcgctgtcg
    11821 cgccgagcgc gcggcgggtg ccgatctcgc gcgtgcgctc gcgaacggtg atccatgcga
    11881 tcgcgagcac gccgacgccg gcgaccagca gcgccgacag gccgatccag cgcacgaaga
    11941 agccgaggcg ttcagcggcg gcgacgcgcg tatcgcccag ctcttgctgg ttcagcaccg
    12001 agaagtcgtc gggccggaag ctgccggtgc gatgccggtc gcgcagcagg tcggtgatgt
    12061 cgcgcaccgc gcccgccatg gccggaaagc tgccgacctc gagcacgagc gcgttgtagt
    12121 gctcgacgtt cagcagccgg cgcatcgcgg cggtgagcgg cacgtagacc tgtgcgtcct
    12181 cgttggtcgc atcgagcccc tggccgcgct cggcgagcac gccgatcacc tcgaagggca
    12241 cccggttgat gaacagccgc tcgcctagcg gcgactgccc atcgtagaga tccttcgcga
    12301 tggtggcccc gagcaggacg acgcgagccg agcgccgcag ctgcgcatcg tcgaaaacct
    12361 cgccctcggc gaccgcccag tgcttgatgc ggaagaaatc gggctcgcag ccgacgaccg
    12421 gcgcgacctt cgacaggccc gccgccttga gcctcaggct cgcactggcg agcgccgacg
    12481 agcgtacgat cgccggcact tcgcgacgca ggctccggta gtcgtcttcc cgcagcgtgg
    12541 tcgcgatatt gccggtgcgc gcccgcccgc cggtgctccg gtcttgcagc gcggcaacga
    12601 cgatcacgtt ggtgccgagc cgcgcgacct ggtcgatcac ctgctggcga ccgccggagg
    12661 cgtagttcac cgcggcgatc gagcccgtga cgccgagcgc ggcgcaggcc atcacgagca
    12721 gcgaacgggt acggtagcgc cagagctggc gccacgccgc gctcgtcagg tgccggtagc
    12781 ggctcagctg cgcagcgcct cgatcggatc gacgttcgcg gctttccatg cggggtagag
    12841 gccaaacacg atgccgaccg ccagcgacag ggccacggcg ccgccgatca tgctccacat
    12901 gtacgccggc ggcagttgct gcagcagggc gccgacggtc gcgccgcctg cgccgatcac
    12961 gatgccgacg gctccaccga gcgccgagat cagcgtggcc tcggcgagga actgcgccag
    13021 tatgtcggca cgtgtcgcgc cgacggcgcg acgtacgccg atctcgcggc ggcgctccga
    13081 cacaccgatc agcatcagac tcatgattac cgcgccgccg atcagggtcg cgatcgcggc
    13141 aacgccggtc acgatcttcg agagcgtact gccgacctgc tggatctgcg ccagcgtcgc
    13201 ccgcgggctg ctgaccgtga agtcgtcctc cgcgggcgga atgatccggt gccgctcgcg
    13261 cagcagcgcg cgcacgtcat cggccacgcg gtcgctgtcg ccctcgttgc gcaactgcac
    13321 gaccagcgtc gacaggtagt cacggttgaa gaggcgcttc gacgcggtcg tgaccgggat
    13381 gaccagcatg ttgtcgaggc tcgcgccgcc cggtcccgca ccgcgcgtcg ccatcacgcc
    13441 cttgacctgg aagggcacgt cggcgatgcg gatcaccttg ccgagcgggt cttcaccctt
    13501 gaacagcacg ctggcgacgt cggcgccgat cacggccaca cgcgcgagcg ctcgctcgtc
    13561 ctcctcggtc aggtgggagc cgctcgcgac ggcctcatcg cggatgtccg cccagttcga
    13621 ggacacgccg agcacgaggg gcacgaccga ctcgtcgcgg taactgacgt cgagatcgaa
    13681 agtctgctgc acctgggcga cgcgcagcac gccgggcacg ccctcggcaa tggccgggcc
    13741 gtccgcgaac ttgagcgagg gctcgaccgt actgaccgac ggcatgccgc gcgtgctgcc
    13801 cgcgccgggg cggatcgtca gcgtgtcgaa cgtgcccagc atgcgcttga actgtcgcag
    13861 ggtctcctgc cgggtggcct ccccgaccga ggccagcgcg gtgagcgcgg cgacgccgac
    13921 ggccacgccg agcatcatga agaaggtgcg cagcttgctg cgggtcagcg agcgccacgc
    13981 ggcaccgatc acgcgcagcc cgctcacgcc tgcacccgtt cgcgccgccg ggtatccgac
    14041 gccaccacgc cgtcgcggat cacgatctcc cgctcggcga acgaggcgac atgctcgtcg
    14101 tgcgtcacga gcacgatagt gcggccctcg cgctgcaggc tcgtcagcag ttgcatgacg
    14161 ctctcgccgg ccgccgagtc caggttgccg gtcggctcgt cggcgagcag cagcggcggg
    14221 tcgttcatca acgctctcgc gatcgcgaca cgctgctgct caccgcccga cagctcggtc
    14281 gcgaaatggt ccgcgcgccc gtcgagcccg acttgcgcca gcagctgtcg cgcccgcgcg
    14341 tgatcgggcg ccgcgccggt gtagagcgtc ggcagctcca cgttctcgag cgcggttgcg
    14401 cgcggcagca gattgaagtt ctggaacacg aagccgatgc gtttcgagcg cacgtgcgag
    14461 cgctcgttgt ctgacagcgc cgagacgttc tcgccgccga ggcgatagct gcccgaagtc
    14521 ggcgtatcga ggcagccgag gatgttgagc agcgacgact tgcccgagcc cgacgcgccg
    14581 cggatgacga cgaactcgcc cggggcgatc gcgagcgaca cgtcgcgcag cgccgtgacc
    14641 ggcgtgccgt cgccgcgggt gtaggtgcgt gagagatgac tcgcttcgat catgcgtcaa
    14701 ttctcctggc gtaccgcgcc gacgagcacg cgctcggtcg gcgcgagccc gcggcggatc
    14761 tcggtgacgc cgccggcgcg agcgccgacg acgacttcgc gccgctcggc gccggcctcg
    14821 gtctgcacga ggacataccg ggtcccctcc tcgcgcagta ccgcctcgtt cggcaccacg
    14881 agcgcctcgc gctgcgcggt ctcgatcgtc acatttgcgg tcatgtcggg cttcagcagt
    14941 tccgccgacc cgtcgatgcc gatcatcacc tcgtagttga cgaccccgga aatgatcgta
    15001 cccttcggcg cgacccgctc gacgtggccg cgaaactcct cggcgggaaa cgcctcgacc
    15061 gtgaagccga ccggattgcc gggggcgacc ccgccgatgt cggtctcgtc gaccatcgcg
    15121 acgagctgca atgcgtcgtc accgatgatc gtgacgaagg tcggcgtcgt gaacgaagcc
    15181 gccacggttt ccccctcctg cgtggtcacg gacgccacgg tgccggcgat cggcgcgcgg
    15241 atcaccgcat agccgagctc ggtctcgacg accgcggcat cgcgccgcgc cttctcgagg
    15301 cgcgcggtcg cctcggcgag cgcgagttca cgatcctcga cctcgctttc cgcgacgagc
    15361 ctctgctcct cgaggcgctg cacgcgcgcg agctcgacgc gcgcgcgggc gacgtcctgc
    15421 tcgatcacgt cgatctgggc acgcgcctgc gcaaggcgcg cttcgatgcc gcgcgagtcg
    15481 atgcgcgcga tcacggcgcc gcggtcgatg tgcgaaccca ccgtcacgtt cagctcccgc
    15541 acgatgccgg acacctgcga cccgacccgc acttcggcgc cgacgcgcag gcgcacgacc
    15601 ccggtcgcga gcacggacga ggcgacggtg cgccgttccg gcgtcaccac gcgcaccgcc
    15661 gcatccgcgg ggccctcgcc cgggcgcgcc tgccaccaat agacaccggc gaccagcacg
    15721 gcgaggaccg cgccggtggc tgcaagcgcg gcggctcgcc tcactcgacc ttcccgtaca
    15781 ccatgaccac gggcttgccg gcgccgcgca cgctctcgac ggtcatcgtc ctgccgtcac
    15841 tgctcagcga gcgcgtctcg gtgcgcacgg ttttcgtgcc cgcgaccgaa ccctccgcgg
    15901 tcgatgtgac caccagcttg tcacccaccc actgagccac ggtctcggtg gcaccgccca
    15961 tcggacccgg gttcgtcacc ggtgccccgg acaggtcgta acgcacttcg cgctcgcttt
    16021 tctggccctg gaagtccgaa gcctcccgca ataccagctg agccgacgac tgcgtgatca
    16081 ccagggtctg ctgcatcgct gccatcatgc cgaggttctc gccgcgcgcg gtatcgagct
    16141 tccaggtccc ggcgaagttc gtcggtgcgt ccgcggcgaa cgcaggcagc gcgccgcaga
    16201 gaatggcgga cagaaggcag gtggcgacgc gattcatctc ggttctccct tgcatcagcc
    16261 aagtataagg gcccggcacg gccggcaagg cggtcgcccg tgactgcaca atggtggcgc
    16321 cgcaggaaat attgccctgc ctctgggcgg ccattcccgg cgggaggatc attccacgcg
    16381 ggccatcgac tccaccccgg agaacgcaat gagaatgaga attccggcgc ccctcccgta
    16441 ccggcttgta ctcctggccg tcgccttggc cgccggcctc gtatccgcgg tcgaaccggc
    16501 cgggccggct cccgctcccg ccgacgcccg cgagctgccc tcgcacatga tcccgaacat
    16561 tcccgatgcg gccgaagcgt actgggcacc ggattcgcgc cacctgattg cgcagacacg
    16621 cgatcgccgt gcggtgccga tgcccaacgg cagcccgggt gcactcacgt ggatattcac
    16681 cgacgacggc acggagatgt ggaatgtgaa cgaccgcggc caggacggct gttcgttctt
    16741 ctttcctgac agccagcgca tcgtctggac ctcgacgcgc gatcgcatgg acctgccggt
    16801 cggcaactgg tccgactcgg acaactatcc gcggggcgcc gaactctaca gctccgacct
    16861 gcacggcggg cacctgcaac gcctgacgaa caatgaatgg tacgaggcgg aggtctccgt
    16921 atcgcccgac ggcaagtgga tcacgttcgg ccgccagatc gacggcaaca tggacatctg
    16981 ggtgatgcgc tcggacggca ccggcaagtt ccaggtcacg cggaccggcg actggcagga
    17041 gggcgcgccg ttcttcatgc cggacagcga acacatcatc ttccgggcct ggcggcgcgc
    17101 cgactacaag aatgtaagac caacgccgat gacgatcttc acgatcaggc gcgacggcac
    17161 cgactggcgc cgccacacct acgacgacgg catgaactgg cacccggtgc cggcacccga
    17221 cgggcggcac tacgtgtacg tcaaggcgct caccgagacg aactgggaga tctacctcgg
    17281 cgatctcgcc ggcggagagc accgtcgcct cacgtatttc gacggcctcg acatcctcgc
    17341 ccatgtctcg cccgatggcc ggaagatgaa ctgggcccgc ggggccggcg cgggattcac
    17401 acagggcttt cgcacccacg tgatggacgt ctcgtcgctc gggatcgggc ccgaacgagc
    17461 ggtgcctttc gatccgtcct ggggtcggcc gatgactgcg ggcacgtcgg actgatcggg
    17521 cacactgccg cgaggaagct gcgtcaaggc cagtggcccg gcagctggaa gcgctcgaac
    17581 cgatgcagcg cctcctcgat ccgcggtttc agcgcgcggc gcggctgccg gccgagccag
    17641 gtccattgct gcacgagaag ccggccgcgc gcgcggtcgg ttttcaggtc gagcgctgcg
    17701 acgatctcgt cgccgaccag caccggctgc gcgaagtagc cgaactggcg cttcgccttg
    17761 ggcacatagg cctcgaagcg atgctggtag ccgaacagca gctgcaatcg ccggcgctgg
    17821 atcacgagcg gatcgaaggg cgagaggata tgcacctgct ctcccggcgc ctgcacacgc
    17881 gcttccagcg cctgcgggct cgcccagtgc gggactttct ccgcgccctc gactaccacc
    17941 gccagcagct cgccgctgcg taccgtcgac tcgataaggc gtcgcaccgc gggcttgcgc
    18001 cgcgcatcga gatggcagat cgaatcgagg ctcacgatgc cctgggcccg taacgcgcaa
    18061 tcgagccggt gcgcgacgat ctcgcgttcg gacgcgagcc tcggcggggc gtcccagccg
    18121 aagtgccgcc cgatgagttc gtaggttttc accatgccgc gccgctcgct caccgtgacg
    18181 acacccttga agaatgcgca ctccagcgcg cgcttcgaag gcttgcggct ggcccacgga
    18241 tggtgcctgt cgaccaggtc gtcatcgtcg atatcgcgga tcgacagcgc accctccgcc
    18301 cggatgcgcg caagcacgcg ccgcaggtcg gtgcgcgtca ccgatccgaa ccagccgatg
    18361 ttcgcctgcc agttgcgccg catcgcgccg aggtgcacgt gcaggtcgcg cgtcggcacg
    18421 taggcgagcg catgcgtcca gcactcgaac acggacttgt cgatcgcctg cgcctggtgc
    18481 aggtgttcgc gccggtaagc cggaatgcgc gtgtacagga tctggtgatg gcagcgctcg
    18541 accacgtgga tcgtgtcgat ctgcacgtag cccaggtgct cgaccgcggc acgagtcgcc
    18601 cccgggccgt caccgaaggg cgcgcagcta tcgaggcgct gtgcgcgcag ccagatgcgc
    18661 cgcgcgagtg aggcactgat gacgacgggc tggcggccgg tgcgcgacat gcagcgcgca
    18721 gtctagcgaa tcagccctcg ccgggcggca gccaggggca ggagtccatt ttctcgacca
    18781 ggtcgaaagc gaacaccatt ttcgccatgc cgcacaacac ggccatgatg acgccgagtt
    18841 cgacgatctc gccgtcgctg taatgcgcgc gcagccgctg gtacagggcc gtcgtgatgt
    18901 ggccgcgcgc attggtcagg accatgacat cggcgagcgc gagcgctgcc ttctcccggt
    18961 ccgtgaacgg gccgtcctca tacccggcga gcgcatcgat cttcgcctgc ggcacgccgc
    19021 cggcgagggc cgcgagggtg tcggagcggt tgcagaacgc gcagccatga acgttcgcga
    19081 gccgcaaccg cacgagttcg acgacgctgc gctcgatgcg cccggaatag aacagcttgc
    19141 ggtagaactc ctcgaggtac cagtcataca cctcgggcgc ctggccgaag acctcgatga
    19201 aggtggtgtc accatgcagc tgcgacgcct ggtcccaggc accacgcatg tgctcaggga
    19261 gttgctcgcg cgacaggcgg gcgagcggcg tatcgggtcg cgtaggcata cgtggctatg
    19321 ctgccacgcc ggatgctaac gcgcgccgga aaatccggcg agttcgcggt tgaatcgccc
    19381 ggcctcctcg aggaacggcg agtgcccgga gtcgtcatat acgaccaggc tcgcctgcgg
    19441 caggcgcgca gccagtgcct gcaatgcggc gatatccatg ctcgcatcgt gagcaccgac
    19501 gacgatgcga acgggcagtg tcagcttgcc ctcgagcccg gaattgtcga ccggcaggcc
    19561 ccgcatcgcc gcacgggcgt acgcgggcag catgaggttc gcgacgagca gctgtcgcgc
    19621 cagcgcatcg ggcagcggct tcgcggtcaa catgcgggcg aaggcttccg ccgcggcaat
    19681 ctgtccggga atgtctggtg tcatcgcggc cgcggagagc tgtgccattc gctcgctccg
    19741 gataccgccg cgcgatgtcg cagacgccag cagcccgccg tggctgccga ccagcagaag
    19801 gccgcgcaag ctgtccgcgc cgtgcgccgc gatgtaatgc agcgcgacgt tgccaccgaa
    19861 cgaccatccg acgagcaagg ggcgatcgag ctgtcgcgcc gtcatcacgc tgcgcacatc
    19921 gtcggcccag cgctcgcgct cgctgtaggc ctccggagcc cacggcttgc ccgagccacc
    19981 gtggccgcgc aggtcgaagg cgacgagatg cagctgcgcg cacgcctgcg gcgtgaactg
    20041 ttcgctgaag ctgagatggc tctgcccgaa gccgtgcagc agcaggacag ccggcgccgc
    20101 cacggtgccg cattcggtca cggcgagcgg cacgccatcg gttgccggca cgaacgaatg
    20161 atgcagctcg gcggcggccg ccggtgcgag cgcagccaac gccagcgcga gcagtacacc
    20221 gggcgcggcc cggcgtggcg acgcgggagt cacctcagtt cgcccagcac ctcgatcgcg
    20281 gcgcgttccg ccgactcgag tgccgcctcc atgccacgat tggcgagcga agtatgctcg
    20341 ccgcagaaga acaggcgctg gtgcggagcg gccatcgccg gcaggtagcg cgtgacctgg
    20401 ccgggtccga atactgccca gtcgccggcg ttccacggat ccatggtcca cgagtgcacg
    20461 gcgccgactt cgagctggcc cttcgcagcc ggacgcaggc gctcgatttc ccgaatgatg
    20521 agttcaccgg ccgccttctg gccgaggcgg tcccaataga gccccgtccg gccccgacca
    20581 tagaccatga agtagccgcg tccgtcgtcc gacctgccga caccctcggc gctgatccag
    20641 ccagccggac cgtcggtcca catcgacggt gggaggccat cctcgagcca gaacggcgtg
    20701 cgggggatca gcacgacctg ggtgagattc tggtacgcga gcctgtgtac ggcctcatgt
    20761 tgcacgccgt tcaagcccgg cgcgatgcgc acgtgccgca gcgtcgaata cggcatcgag
    20821 ctgatgatcg ctcgcgcgcg gatccgttca cccgactcga gtgtcacgac tccgctgtcg
    20881 gcgttcgctt cgatccccac gacgcgcgca ccgaggcgca cgtcgccctt caacatgcgg
    20941 gccatggctt ccggcaaccg ctgattgccg cccgtgatgc gatagctggc ccgtttggtg
    21001 gatacggcac tcgatgcgcg tccccacgag gcgatgaacg cgaacatgag cacggagacg
    21061 tcatgtgccg tatcgccgta ggatggcacg gtactgaagg ccaggtcgat cacgcgctga
    21121 tcgacaccct tggcaagcag gaagtcgttc agcgaaatat cgtcggcacg gtgcgacggc
    21181 aggatccagt ctgaaaacga cttgagcgga ttgaggggtg caaacagctt gcccccatac
    21241 gcccacggca tcgtgttctt gtcgacctcc ggaaacacgt tgtgcggtga acgcggccag
    21301 tcttccgggg aaatgatctt gccgtcgagc acgagcacct ggcccggact gcgcgtcgtc
    21361 gtcgcgcgaa ggtcggtcaa cccgaccccg cacttctccg cggcggcaat ccagcggcca
    21421 tatcccgggg tcgcggagct tccccccaat tcgggccgac ccggaaggtc gtaccgggta
    21481 tacacccgcc caccgatccg ctgccgacct tcgagcacgg tgacgcgcag ccctgcctct
    21541 tcgagcaggc cggcggcacc gagcccggaa agcccggcac cgatgacgat cacgtcagca
    21601 acggactgcg cctgcgcgta ggcgacggag cggtacagcg ccgcgccggt cagcgccgcc
    21661 gcgccgcctg cgatgaactc acgacggttg gttgtcatct cgcagcctcc ccgagccagg
    21721 cccgagtgca ggcgccccgg ggggcgccgc agccgctgat acgagcgcct tgcggcggcc
    21781 gatcaccttg tcctgccacc tggctcatgg aggccttcgc ttcagtactc gaaacggacg
    21841 cgaatgccga attcacgctt ctcgaacggc tgcacgagcg cgccgacctg cgtcaagttg
    21901 ttcgggagcg gtgccgtgtc gacccagtcc acggcgcggc gccccgtgcg ccagccttct
    21961 tcgttgaggc agttgcggca gaacagctcg acgttcaggc catcttcgcg ggcgaagcca
    22021 atccgcccgt tcagcagcga gtaggcgccg acccacgcga gattggtttc gtcgagatag
    22081 gccttgccct ggtaagaggc gtcaccgcgc aggtaccact cccagtcgga gttcagcgtg
    22141 tcggtccagg tggccgacag cgcgaaagtc tcct
//
LOCUS       DAOQVY010000032        22054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_2773_length_22054_cov_5.580572, whole genome shotgun sequence.
ACCESSION   DAOQVY010000032 DAOQVY010000000
VERSION     DAOQVY010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 22054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22054
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2773_length_22054_cov_5.580572"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..741)
                     /gene="rnt"
                     /locus_tag="PKN91_04775"
     CDS             complement(49..741)
                     /gene="rnt"
                     /locus_tag="PKN91_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HNS27421.1"
                     /translation="MSRIRMAHRFRGFLPVVIDVETGGFNSATDALLEIAAVMIELDE
                     NGNLCRGESHSYHVQPFAGANIEPAALAVTGIDPHHPLRPAIPERDALQRVFREVRAA
                     VRAADCRRAVLVGHNAAFDLGFLNAAVARTDIKRNPFHPFSCFDTATLAGVALGQTVL
                     AKATVVANIDWDASSAHSARYDAERTADLFCSIANASRDSYLRAEERARLLGWIEPAI
                     PVSDAGTDADPA"
     gene            complement(738..1784)
                     /gene="pyrC"
                     /locus_tag="PKN91_04780"
     CDS             complement(738..1784)
                     /gene="pyrC"
                     /locus_tag="PKN91_04780"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNS27422.1"
                     /translation="MTRLTIARPDDWHLHLRDGAALAAVLPHTAARFARAIVMPNLKP
                     PVTTTAEAAAYRARVLAALPAGARFEPLMTLYLTDHTSTAEIERASASGIVHGCKLYP
                     AGATTHSDAGVTDIRRLDDTLARMAELGLPLQVHGEVTDQDVDIFDREARFIDAVLAP
                     LIERIPRLKVVFEHITTRSASEFVRSAREGVAATITPHHLLLARNALLAGGIRPHLYC
                     LPILKTETDRRALIEAATSGNPRYFLGTDSAPHARHTKETACGCAGIYSAHAGIELYA
                     EAFEAAGALPRLEAFASHFGADYYGLPRNRDTITLVKAPWSVPADYAYGADTLVPWYA
                     GEALRWQLATECSQ"
     gene            complement(1781..3268)
                     /locus_tag="PKN91_04785"
     CDS             complement(1781..3268)
                     /locus_tag="PKN91_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HNS27423.1"
                     /translation="MIRRRLDAAALANLDVLLIIAPLDAGKAPAALAGAWPRLRAGRA
                     PERGTVRVTHLPNQPGTRIIAGFAPGPGTFARLALAGRMCRELGDGAWRRVGVLSLGG
                     GTAQADVEAMLAALHAHAFRLPAYGRRAQDMQRDKLRQLGEIRVFSETPPPLARIAAT
                     ARGTNLARWLTALPPNKLDAPAYRRLMGTLAREHDIRVRWYDERALRRLGAGCFLAVA
                     AGNARRNAGIAHLTWRPPARGARARGRAPDVALIGKGVLFDTGGNNLKPHRGMLDMHT
                     DMSGSAVALATLLGLAELRAPIAADARLAITENRIGPAAYRPQDVLRAMNGVTIQVVH
                     TDAEGRLVLADTLALAARARPRLMLDFATLTGVCEYALTERMSGLFTNAPRLLPQLAA
                     AGAQSGERVWNFPMDADFDTDLESTVADIAQCSAESKGDHIHGARLLSRFVPKGTPWA
                     HLDLSAAVRKGGLGHVPSDITGFGVRYALELLLGTDILAAAGATK"
     gene            3487..6150
                     /gene="aceE"
                     /locus_tag="PKN91_04790"
     CDS             3487..6150
                     /gene="aceE"
                     /locus_tag="PKN91_04790"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNS27424.1"
                     /translation="MTDIPRFEDLDPTETREWIESIDSVLKSHGPERAYFLLEKLIDH
                     ARRSGTYLPFRPNTAYLNTIAPGQEPDYPGNRALERRVEAYIRWNAMAMVVHANRISS
                     EYGGHLSTYASALTLYEVGFNHFWRAPSDRHPGDMIFIQGHASPGIYSRAYVEGRLTE
                     EQLRNFRQEVAGNGLPSYPHPWLMPDFWQYPTVSMGLGPMQAIFQARFTRYLEHRGIV
                     KPSDRKVWAFLGDGEMDEPESLGAITMPVREKLDNLVFVINCNLQRLDGPVRGNGKII
                     QELEAAFLGAGWNVIKVLWGSRWDPLLAKDHKGLLRRTMEECVDGEYQNFKAKGGAYT
                     REHFFGKHPELKAMVANLSDEEIWRLNRGGHDPRKVYAAYAAAVAHKGRPTVILAKTV
                     KGFGLGKSAEGQMMTHQAKKLSDEDLLIFRDRFHIPISDDEIARLPFCRPAEDSEEMR
                     YMRERRMAHGGYLPVRREVAPPLAIPALETFQAMLEGTGEREISTTMAFVRILTALLK
                     DKNIGQNIVPIVPDEARTFGMEGLFRQIGIYSSVGQLYTPVDAEQLASYREIKTGQMI
                     EEGINEAGALCSWIAAGTAYANHGINMVPFYIYYSMFGYQRAGDFIWAAGDQQTRGFL
                     IGATAGRTTLAGEGLQHQDGHSPLLFSVVPNCVAYDPSFAYELAVIIHDGLRRMFVEH
                     EPVFYYITTMNENYVQPAMPRGVEQGILKGLYLLQIGGRGRVRATLLGSGTILREVIA
                     AAELLEKDFNIPADVFSAPSFGELRRDALECERWNMLHPQDEPRVPYVQQCFKDREGP
                     VIAATDYMRSVPDQIRQWIGGTYVTLGTDGYGRSDARAPLRRHFEVDRHFIVLAALKA
                     LADDGKIDHATAAAAITRYGIDIDKPDPLKS"
     gene            6164..7534
                     /locus_tag="PKN91_04795"
     CDS             6164..7534
                     /locus_tag="PKN91_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNS27425.1"
                     /translation="MTRLIEMRVPDMGEFKDVAVIEVLVKPGDTVAVDTALATLETDK
                     ATLDVPATAAGVIETVHIQQGGVVNPGDLVVTVRGADAPAAAGDTSAAPAPAAAPAEA
                     PAGPAAAAAPPAPTPPPAAPPTAQAAPAPAAAPAARPAAPVQIDEAGFSRAHAGPSVR
                     KLARELGVDLVQVRGSGLKDRITADDVKAHVKQALGAIAGAGTAAQGGGALPAVPSVD
                     FAQFGPVEVKPLGRIQKISGARVHASWVNLPHVTQFDEADITDLEATRAKLKGKLEKS
                     GVKLTLLAFIMRACVRTLKEFPQFNASLDATRANLVFKKYFHIGFAADTPNGLLVPVV
                     RDADRKDVYEIARALAELSAQARAGKLPPSDMQGGSFTISSLGGIGGTAFTPIINAPE
                     VAILGVSRSITKPVYRDGGFVPRLMVPLSLSYDHRVIDGALAARFTTRLAEHLADPQG
                     LLEAVP"
     gene            7531..9312
                     /gene="lpdA"
                     /locus_tag="PKN91_04800"
     CDS             7531..9312
                     /gene="lpdA"
                     /locus_tag="PKN91_04800"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNS27426.1"
                     /translation="MSIRDVLVPDLGEFKDVPVIDVLAAPGDRLEVDTPLITLETDKA
                     TMDVPSPVAGTLVELTVRKGERVSAGVLIARVETAPEDRAGASGSAGAQTAAASPATD
                     SDSLKTKVLLQLAGVPEEIETDRSVQLLVLGAGPGGYTAAFRAADLGLAVTLVDRWPR
                     LGGVCLNVGCIPSKALLHAARVIEEAHAMGAHGVEFGAPRIDKDRLRDWKSGVVKRLT
                     GGLSMLAKQRKVDVVRGSGRFLGPHVVEVTREDGGSERIRFEQCIVAAGSEPMRLPFA
                     PHDPRVMDSTGALELTDIPQRLLVVGGGIIGLEMACVYDALGSKVSVVELTKQLMPGC
                     DPDLVRPLEKRLRARYERILTGTKVTAMAAEADGIRVSFEGADGAQPATYDRVLIAVG
                     RVPNGGRLEAEAAGIAVDDRGFIAVDKQMRTNVPHIFAIGDIVGQPMLAHKAMHEAKV
                     AAEVAAGGKHAFDARVIPSVAYTDPEVAWVGLTETEARTRGIPVAKGAFPWVASGRSL
                     SMARDEGFTKLLFDPQTRRVLGAGIVGTNAGELIAELALAIEMGADAADIALTVHPHP
                     TLSETVGLAAEAFEGTLTDLYVPKKSQ"
     gene            complement(9288..9914)
                     /gene="nth"
                     /locus_tag="PKN91_04805"
     CDS             complement(9288..9914)
                     /gene="nth"
                     /locus_tag="PKN91_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNS27427.1"
                     /translation="MTRQRRRAIFVRLRQANPHPTTELEYSTPFELLVAVVLSAQATD
                     RSVNAATRKLFPVANTPAAILALGASGLSDYVRTIGLYNSKAKHIIETCRILIERHGG
                     EVPHDRAALEALPGVGRKTANVILNTAFGEPTIAVDTHIFRVANRTGLAVGRTPRQVE
                     DILVATTPAEFARDAHHWLILHGRYVCTARKPRCADCVIARYCDFFGT"
     gene            complement(9935..10477)
                     /locus_tag="PKN91_04810"
     CDS             complement(9935..10477)
                     /locus_tag="PKN91_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNS27428.1"
                     /translation="MTLAESRADAIDALLPQTQCTRCGYDGCRPYAEAIASGAAPINQ
                     CPPGGARTIAALAQLLGREPLPLNPANGSEQPERVAVIDEALCIGCAKCLPPCPVDAI
                     VGAARHMHTIVADLCTGCELCVAPCPVDCIRMVERPVSSPRPPAAADNRLRYALRNAR
                     VARRAAERDALLAARKGLAG"
     gene            complement(10474..12516)
                     /gene="metG"
                     /locus_tag="PKN91_04815"
     CDS             complement(10474..12516)
                     /gene="metG"
                     /locus_tag="PKN91_04815"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNS27429.1"
                     /translation="MRRILVTSALPYANGPLHLGHIIEAVQTDIWVRLQKMLGHDCLY
                     VCAEDAHGTPIMIRAQGEGVTPEALIERVAAEHARDYAGFLIGHDQFHSTHSPENRAL
                     AESMYLRLRDAGHIARRPVRQAYDEQAGMFLPDRYVRGGCPRCGTPEQYGDSCEHCGA
                     TYSPADLVNPVSTISGTTPVWRESDHIFFRLGAFEEMLREWLTTGALQPGVRAKLAEW
                     FDAGLKDWDISRDHPYFGFEIPDESGKFFYVWFDAPIGYLASLRALCDRTGRSFDEYM
                     RPDSSAELHHFIGKDISYFHTLFWPAVLHGSGFRKPTAVYVHGFLTVNGQKMSKSRGT
                     FITAQRYLEHLPAEPLRYYFAAKLGPDLDDIDLNLDDLVARVNSDIVGKLVNIASRCA
                     GFIARGGGRLAARLPEPALYQEFADARGRIAALFDARDYSAAVREVMLLADRANQYVD
                     HHKPWILAKDPARAAEVLAVATQGINLFRVLMTYLAPVLPRIALDAGTFLGTPIDRWD
                     AIATPLLDVAINEFKPLATRLDRSSVAALIDAGAEAQAPAATAGAPAVAASAPAAAAR
                     APAAATVDIKDFARLDLRVARVIAASTVEGSDKLLRLELDLGGERRTVFSGIRAAYEP
                     ARLEGRLVLMVANLAPRKMRFGTSEGMVLCASGADDSQGIYLLAPDDGAVPGMKVT"
     gene            complement(12584..13645)
                     /locus_tag="PKN91_04820"
     CDS             complement(12584..13645)
                     /locus_tag="PKN91_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNS27430.1"
                     /translation="MARIDALLAEILDRKGSDLHFLAGDPPRIRLFGDLSPLRPERLD
                     AAAVKETLYEIMPRQAVERFEAKDGADFAYTLPGRGRFRVNVLRHLNGMGAVFRAIPS
                     KALTLDELQLPQAVRQLCRANNGLILVTGKTGSGKSTTLAAMIDDINRREKGHILTVE
                     DPIEFVHERKNCLISQREIGVHSPSFAAALHSALREDPDVILVGELRDLETMSIAVTA
                     AEMGILVMGTLHTNGAAPTVDRMVNAFPSDKQSHVRTMLSTSLRGVISQQLLQRADKQ
                     GRVAALEILVNTSAVSNLIRQGKLDQLETVMQSGAQAGMRTMDSAIEQLLRERAITGK
                     EAYKKGINKAKFDPVKDQG"
     gene            complement(13656..14774)
                     /locus_tag="PKN91_04825"
     CDS             complement(13656..14774)
                     /locus_tag="PKN91_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNS27431.1"
                     /translation="MARIDAFLKLGMQQGCSDLHLAVGVPPMLRMHGDLMPIKFRELR
                     DTELEGYITEILTRSQAEHFAQGHDLDFSYVSGEGGRFRVNVYRKDTGVGAAFRAIPT
                     EIPTIDSLNLPPIVKKLCDFHQGMILVTGSTGTGKSTTLAAMIDLLNINRRLNIISLE
                     DPIEFVHRSKQSQVIQRELGTHIPSFAEGVRAAMREDPDVILVGELRDAETISMAMTA
                     AETGHLVLGTLHTTSATKTIDRVIDALPVEEREQTKSFLAQSLLAVVTQVLVKSVDGR
                     ARRAVCEVMVMTKAIAKLVQSDQTHQLPSQLQTGRDQGMQLLDQALLAAVNAKEIDPD
                     DAYNYATDKRLFQKFVTDTSILPKLDSPAPASGATGGG"
     gene            complement(14888..17326)
                     /locus_tag="PKN91_04830"
     CDS             complement(14888..17326)
                     /locus_tag="PKN91_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNS27432.1"
                     /translation="MSFFSNLRADRFITQIIATKDPNDPETQKAAARLKSLGGAAVES
                     LFAALADADKNATVVFVDVLASIVNAKNFHLFTRGLVEGGPRVVAGISWALSSSRAYP
                     PHLLLEALQLEGISKSALVDIIATQKQRFSVRELLNAAYAQEPNEKAALFRIINDMAD
                     ASALPELIGRLQGKDPIARLHLLNILSRFNTPAVQSALQDQLKDNNKLVRAAALAALH
                     KMDGPIDVQRVCEMLRDPEIDVQNKAIDVVIKANDPETIKYLVPVLKDENEFARRAAV
                     EVLNEVGTAKSVKFLLDAIKDDDWWVRSRAADALGKIGGPRVVDAVLQLIKDDDEDIR
                     RAAIEILNQTKDERAVAHLIEATRDKDWWVSERAVDALAEIGSKRAVPRIVELLQTAP
                     ARSIPVAIRALAKLGDHKVIETLLPFLARPEKEIRVEAMGTLAKLADERRAEAIRVHL
                     QAQTALPDPTIAQAAQHALTELDARFGSMLSSSGSGRGTLAATPVPGTVGHAPQRPTE
                     AARTLLMSEAEVAQVVRQAEASTQPKLDISTLNPGDVIEGRYKYIERIGRGAFGTVLL
                     MEDTVVDERLILKFLNPNVAQDEEIMKRFVHELRYSRKITHQNVIRIYDFLYIQGHYA
                     ISMEYFPSHTLGAEIVGEKPMELKRALGFAIDICTGMAVAHQVGVVHRDLKPANLLIN
                     NEGLLKIVDFGVAAAQREGDTQLTKTGYVIGSPKYMAPEQILGKKVDQRADVYALGVI
                     LYEMLTGVPPYSRGDHMAVMYQHVQGKARTVKDVNPSLPEGLSEVVMKAMAVDKTKRY
                     QTMDEFKAALSRFL"
     gene            17501..18595
                     /gene="apbC"
                     /locus_tag="PKN91_04835"
     CDS             17501..18595
                     /gene="apbC"
                     /locus_tag="PKN91_04835"
                     /inference="COORDINATES: protein motif:HMM:NF008669.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HNS27433.1"
                     /translation="MPNNQDAALLAALATFVDPYAGETLATLQAVEHATVAAGRANVR
                     LRYGFPVGGYAHELQPLLAGHLAAAGIALPLELELAADIRAHAVQRQLKPMASVSNVV
                     AVASGKGGVGKSTVAANLALAWAVQGARVGILDADIYGPSQPQMLGLAGERPEIAPGK
                     RIRALEAHGIVAMSIGFLIDAAQPMVWRGPMVTQALTQLLNDTLWGQLDYLVVDMPPG
                     TGDIQLTLAQRVPVAGAVIVTTPQHIAVADARKGLRMFEKVSVPVLGIVENMSLHVCS
                     NCGHAEAIFGEGGGDEMAREYGVALLGRLPLDARIREEADGGRPSVIAAPDSPRAAAY
                     LTMARRTAAVLARRERDRSTTFPNIVVEET"
     gene            18600..19166
                     /gene="dcd"
                     /locus_tag="PKN91_04840"
     CDS             18600..19166
                     /gene="dcd"
                     /locus_tag="PKN91_04840"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005889365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNS27434.1"
                     /translation="MSIKADKWIRRMACGQKMIEPFEPGQVREVDGRRIVSYGTSSYG
                     YDVRCSNEFKVFTNINSTIVDPKGFDAKSFVDMQADVCIIPPNSFALARTVEYFRIPR
                     NTLVVCLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPAKIYANEGVAQMLFF
                     ESDEVCETSYKDRGGKYQGQRGVTLPKT"
     gene            complement(19163..19927)
                     /locus_tag="PKN91_04845"
     CDS             complement(19163..19927)
                     /locus_tag="PKN91_04845"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNS27435.1"
                     /translation="MDPRPADTRLWRASALASLAMAALALAGASASLAADWHPTPFHA
                     TYAVQWKGLNAGTSTLELRRTGVDTWAYVSRSNARGIFRIAIPDTVEQDSELRFVAGE
                     PQPLRFRGDDGGDATDRDVALDFDWQHMRITGTAENQPVDLAMPPDIHDSMTVQLAQM
                     RAAAAGVTAVAFHTVDKDEIKEYDFTRSGTQKILTSLGELETVIYESRRRGSKRMIRL
                     WMAPSLDYLPVRAERFRADKIEFAMAIRELRRDEAL"
     gene            complement(19908..20579)
                     /gene="purN"
                     /locus_tag="PKN91_04850"
     CDS             complement(19908..20579)
                     /gene="purN"
                     /locus_tag="PKN91_04850"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNS27436.1"
                     /translation="MTAPPPLRLAILISGRGSNMAAIARACATRTINARVACVGTDRA
                     DAAGLDTARALGIDAFAVLPAAYASRPEFETALAARLDECRPDLVVLAGFMRILGDAF
                     VARYRGRMLNIHPSLLPKYKGLHTHRRALAAGDPMHGCSVHFVTEELDGGPVVLQGRL
                     RVAPGEDEQRLSARVQALEHIVYPRAIGWIAAGRLAWRDDRVWLDDKLLTAPITEDFD
                     GPATG"
     gene            complement(20576..21613)
                     /gene="purM"
                     /locus_tag="PKN91_04855"
     CDS             complement(20576..21613)
                     /gene="purM"
                     /locus_tag="PKN91_04855"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNS27437.1"
                     /translation="MPVPPPPITYRDAGVDIDAGDALVERIKPLVRRTQRREVLGGIG
                     GFGALVEVPLDRYVRPVLVSGTDGVGTKLRLAIETGRHETIGIDLVAMCANDVAVQGA
                     EPLYFLDYYATGKLDVDVAATVISGIAAGCALAGAALVGGETAEMPGMYAGDDYDLAG
                     FCVGIVEKDAIIDGSRVTTGDAVIGLASSGAHSNGYSLIRKLIGVAGANAGTQLDGQP
                     LFDRLLAPTRIYVRSLLELARRIPVHGLAHITGGGLTDNIPRTLPAGLEVVLSRRTWH
                     RDPLFDWLQRTGSLPDAEMHRTFNCGIGMVVIVAAADVDASLALLRAHGEVASVIGTV
                     RGGTAGVVIEA"
     gene            21632..>22054
                     /locus_tag="PKN91_04860"
     CDS             21632..>22054
                     /locus_tag="PKN91_04860"
                     /inference="COORDINATES: protein motif:HMM:NF021367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HNS27438.1"
                     /translation="MHAAIRCVALALLAGLPTAEAARTVRVYDVAVREAASADVPAAA
                     MRTALVRATGQRAAASDPALAMLVADAPRYVRLSRPGADGRTEVSLDGDAIDSAILAA
                     GRVLWSAERPLTLVVLEVAPGAVIDAGVQAAIEAAAGDR"
BASE COUNT         3412 a         7332 c         7750 g         3560 t
ORIGIN      
        1 gaaaaccagc gctcatcaag tgccgcaaag actctgccgc ccagactcct aggccggatc
       61 ggcgtcggtg ccggcgtcgc tgaccgggat cgccggctcg atccagccga gcaggcgcgc
      121 gcgctcctcg gcgcgcaggt agctgtcccg cgaggcgttg gcaatcgagc agaacagatc
      181 ggcggtgcgc tcggcgtcgt agcgcgccga gtgcgccgaa ctcgcgtccc agtcgatgtt
      241 ggcgaccacc gtcgcctttg cgagcacggt ctggccgagc gccacgcccg caagggtggc
      301 cgtgtcgaag cacgagaacg ggtggaacgg gttgcgtttg atgtcggtgc gcgccacggc
      361 cgcattcagg aaaccaagat cgaaggcggc gttgtggccc accagcacgg cgcgccggca
      421 gtccgccgcg cgcacggcgg cacggacctc gcgaaagacc cgctgcagcg cgtcgcgctc
      481 cgggatggca gggcgtagcg ggtggtgcgg atcgatgccc gtgaccgcga gcgccgcggg
      541 ctcgatgttc gcgcccgcga acggctgcac gtgatagctg tggctctcgc cgcgacacag
      601 gttgccgttc tcgtcgagtt cgatcattac cgcggcgatt tcgagcagcg cgtcggtcgc
      661 ggaattgaag ccgccggtct cgacgtcgat caccaccggc aggaagccgc ggaaacggtg
      721 cgccatccgg atcctgctca ctggctgcac tccgtcgcga gctgccagcg caatgcctca
      781 ccggcgtacc agggcacgag cgtgtcggca ccgtaggcat agtccgccgg cacggaccac
      841 ggcgccttca ccagcgtgat cgtgtcgcga ttgcgcggca gaccgtagta gtcggcaccg
      901 aaatgcgagg cgaaggcctc gagccgcggc aaggcgccgg cggcctcgaa cgcctcggca
      961 tagagttcga tgccggcatg cgccgagtag atgccggcgc agccgcacgc ggtttccttc
     1021 gtgtgccggg catgcggcgc gctgtcggtg ccgaggaagt agcgcggatt gccgctggtc
     1081 gcggcctcga tcaatgcgcg ccggtcggtt tccgtcttga ggatcggcag gcagtaaagg
     1141 tgcgggcgta tgccgccggc gagcagcgca ttgcgcgcga gcagcaggtg atggggcgtg
     1201 atggtcgcgg ccacgccctc ccgcgccgag cgcacgaatt cgctcgccga acgcgtcgtg
     1261 atgtgctcga agacgacctt gaggcgcggg atgcgctcga tgagcggcgc cagcaccgcg
     1321 tcgatgaagc gcgcctcgcg atcaaaaatg tcgacatcct ggtcggtgac ctcgccgtgc
     1381 acctgcagcg gcaggccgag ctccgccatg cgcgcgagcg tgtcgtcgag ccggcgaatg
     1441 tccgtcaccc cggcgtcgga gtgcgtggtc gcgccggccg gatagagctt gcagccatgc
     1501 acgatgccgc ttgcgctcgc gcgctcgatc tcggcggtgc tcgtgtggtc ggtcaggtac
     1561 agggtcatca gcggctcgaa gcgtgcgccg gcgggcagcg ccgcgaggac gcgcgcgcgg
     1621 taggccgcgg cctcggcggt cgtcgtcacc ggtggcttca ggttcggcat cacgatggcg
     1681 cgcgcaaagc gcgcggccgt gtgcggcagc accgccgcga gcgccgcgcc gtcgcgcagg
     1741 tgcaggtgcc agtcgtcggg ccgcgcgatc gtgaggcggg tcatttagtg gcccccgcag
     1801 cggccaggat gtcggtacca agcagcagtt cgagcgcgta gcgcacgccg aagccggtga
     1861 tgtcgctggg cacgtgcccg agtcctccct tgcgaacggc ggccgacagg tcgagatgcg
     1921 cccacggcgt gcccttggga acgaagcgtg acagaaggcg ggcaccgtgg atgtgatcac
     1981 ccttcgattc cgccgaacac tgtgcgatgt cggccaccgt gctctcgagg tcggtgtcga
     2041 agtcggcgtc catcgggaaa ttccacacgc gctcgccgct ttgcgcgccc gccgcggcaa
     2101 gctgcggcag cagccggggc gcgttggtga acaggccgct cattcgctcg gtgagcgcgt
     2161 actcgcacac gccggtcagg gtcgcgaagt cgagcatcag gcgaggccgc gcccgtgcgg
     2221 cgagcgccag cgtgtcggcg agcaccaggc gcccctcggc gtcggtatgc acgacctgga
     2281 tcgtgacgcc attcatcgca cgcagcacgt cctgcgggcg ataggccgcc ggaccgatcc
     2341 tgttctcggt gatcgcgagc cgggcatcgg ccgcgatggg cgcgcgcagt tcggcaagcc
     2401 cgagcagcgt cgcgagcgcc acggcactgc ccgacatgtc agtgtgcatg tcgagcatgc
     2461 cgcgatgggg cttcaggttg ttgccgccgg tatcgaacag tacgcccttg ccgatgagtg
     2521 caacgtcggg cgcgcgcccg cgcgccctgg cgccccgggc gggcgggcgc cacgtgaggt
     2581 gcgcgatgcc ggcgttgcgc ctggcattgc cggcggccac ggcgaggaag caacccgcgc
     2641 cgaggcggcg cagcgcgcgc tcgtcgtacc agcggaccct gatgtcgtgc tcgcgcgcga
     2701 gcgtgcccat caggcggcgg taggctggcg cgtcgagctt gttcggcggc agcgccgtca
     2761 accagcgcgc gagattggtg ccgcgggctg tggcggcaat gcgcgcgagc ggcggcggag
     2821 tctcgctgaa cacgcgaatc tcgccgagct ggcgcagctt gtcccgctgc atgtcctgcg
     2881 cgcgccggcc ataggcgggc aggcggaagg cgtgcgcgtg caacgcggcg agcatcgcct
     2941 cgacgtcggc ctgcgcagta ccgccgccga gcgacagcac gcccacgcgt cgccatgcac
     3001 cgtcgccgag ttcgcggcac atcctgccgg cgagcgcgag gcgcgcgaag gtcccgggtc
     3061 cgggggcaaa gcccgcgatg atgcgtgtgc caggctggtt gggcaggtgt gtgacgcgca
     3121 ccgtgccgcg ctccggcgcg cggcccgcgc gcagtcgcgg ccacgcgccc gcgagtgcgg
     3181 cgggcgcctt gcccgcatcg agaggtgcaa tgatgagcag cacgtcgaga ttcgcgagcg
     3241 cagccgcatc gagcctgcgc cggatcactc gcgtggctgt cgtctcgagg gcggcggttg
     3301 gcgatgtcga tgcaggcaac ggaattccgc agggatggcg tcgacggtcc gtctgcttga
     3361 ccgtcactcg cgaaaaccgg atcatgacgc gcgcttcacg ggtgctcacg gaaacgcggc
     3421 gccgtgcatg ttaacacagg ccgcgggcag ctcttcacct gctgccatat cgggatccac
     3481 gacacgatga ccgacattcc caggttcgaa gacctcgacc cgaccgaaac ccgggaatgg
     3541 atcgaatcaa tcgattcggt actgaaatcg cacgggcccg agcgcgccta tttcctgctc
     3601 gagaaactca tcgaccatgc gcgtcgctcc ggcacctacc tgccgttccg tcccaacacc
     3661 gcctatctca atacgatcgc gcccgggcag gagcccgact accccggcaa ccgcgcgctc
     3721 gagcgccgcg tcgaggcgta catccgctgg aacgcgatgg cgatggtcgt gcatgcgaac
     3781 cgcatctcgt cggagtacgg cgggcacctg tcgacctacg cgtccgcgct gaccctctac
     3841 gaagtcggct tcaaccattt ctggcgcgcg ccgtccgatc ggcacccggg cgacatgatc
     3901 ttcatccagg gccacgcctc gcccggcatc tactcccggg cctatgtcga gggccggctc
     3961 accgaggagc agttgcgcaa cttccgccag gaggtggccg gcaacggatt gccgtcctat
     4021 ccgcatccgt ggctgatgcc cgatttctgg cagtacccga ccgtgtcgat gggactcggg
     4081 ccgatgcagg cgatcttcca ggcgcgcttc acgcgctatc tcgaacatcg cggcatcgtg
     4141 aagccctccg atcgcaaagt gtgggcattc ctcggcgatg gcgagatgga cgagccggag
     4201 tcgctcggcg cgatcaccat gccggtgcgc gagaagctgg acaacctggt gttcgtgatc
     4261 aactgcaacc tgcagcgcct cgacggtccg gtgcgcggca acggcaagat catccaggag
     4321 ctcgaggcgg ccttcctcgg cgcgggctgg aacgtgatca aggtgctgtg gggatcgcgc
     4381 tgggatccgc tgctcgcgaa ggaccacaag ggcctgctgc gccggacgat ggaagagtgc
     4441 gtcgacggcg agtaccagaa cttcaaggcg aagggcggcg cctacacgcg cgagcatttc
     4501 ttcggcaagc atcccgaact gaaggcgatg gtcgcgaacc tgtcggacga ggagatctgg
     4561 cgcctgaatc gcggcggcca cgacccgcgc aaggtgtacg cggcctatgc ggccgcagtc
     4621 gcgcacaagg gccggccgac cgtgattctc gcgaagaccg tgaagggctt cggtctcggc
     4681 aaatcggccg agggccagat gatgacccac caggcgaaga agctgtcgga cgaggacctg
     4741 ctgatcttcc gcgaccgctt ccacattccg atctccgacg acgagatcgc gcgcctgccg
     4801 ttctgtcgcc cggccgagga cagcgaggag atgcgctata tgcgcgagcg gcgcatggcg
     4861 cacggcggct acctgccggt gcgccgcgaa gtcgcgcccc cgctcgcgat ccccgcgctc
     4921 gagactttcc aggcgatgct ggagggtacc ggcgagcgcg agatctcgac cacgatggcc
     4981 ttcgtgcgca tcctcaccgc gttgctgaag gacaagaaca tcggccagaa catcgtgccg
     5041 atcgtgcccg atgaggcgcg caccttcggc atggaaggcc tctttcgcca gatcggcatc
     5101 tattcatcgg tcggccagct gtacacgccg gtcgacgccg agcagctcgc gtcctaccgc
     5161 gagatcaaga ccgggcagat gatcgaggag ggcatcaacg aggcgggcgc gctgtgctcg
     5221 tggatcgccg cgggcaccgc gtacgcgaac cacggcatca acatggtgcc cttctacatc
     5281 tactactcga tgttcggtta ccagcgcgcg ggcgatttca tctgggccgc gggcgaccag
     5341 cagacgcgcg gcttcctgat cggcgccacg gcgggccgca ccacgcttgc cggtgagggt
     5401 ctgcaacacc aggacggtca cagtcccctg ctgttcagcg tcgtgccgaa ctgcgtcgcc
     5461 tacgatccaa gctttgccta cgaacttgcc gtcatcatcc acgatggact gcgccgcatg
     5521 ttcgtcgagc atgagccggt cttctattac atcaccacga tgaacgagaa ctacgtgcag
     5581 ccggcgatgc cgcgcggcgt ggagcagggc atcctgaagg gcctgtacct gctgcagatc
     5641 ggtggtcgcg gccgtgtacg cgcgacgctg ctcggctccg gtaccatcct gcgcgaggtg
     5701 atcgccgcgg ccgaactgct cgagaaggac ttcaatatcc cggccgacgt gttctcggcg
     5761 ccgtccttcg gcgaactgcg ccgggacgcg ctcgagtgcg agcgctggaa catgctgcat
     5821 ccccaggacg agccgcgtgt gccctacgtg cagcagtgct tcaaggaccg cgagggcccg
     5881 gtgatcgccg cgaccgacta catgcgcagc gtgccggacc agatccgcca gtggatcggc
     5941 ggcacctacg tcaccctcgg taccgatggc tacggtcgca gcgacgcccg cgctccgctt
     6001 cgccggcact tcgaggtcga tcgccatttc atcgtgctcg ccgcgctcaa ggcgctggcg
     6061 gacgatggca agatcgatca tgcgacggct gcggcggcga ttacccgtta cggaatcgat
     6121 atcgacaaac cggatccgct gaagtcgtga gcgagggcag gccatgaccc ggctcatcga
     6181 aatgcgcgtg cccgacatgg gcgagttcaa ggacgtcgcg gtcatcgaag tcctcgtgaa
     6241 gccgggcgac accgtcgccg tcgacaccgc gctcgccacg ctcgagaccg acaaggcgac
     6301 gctggacgtg ccggccaccg cggccggcgt catcgagacc gtgcacatcc agcagggggg
     6361 tgtcgtcaac ccgggcgatc tcgtggtgac ggtgcgcggc gccgatgcgc ccgcggcagc
     6421 gggggacacc tccgctgcac cggcacctgc agccgcgcct gccgaggcgc ccgccgggcc
     6481 tgccgcggcg gcggctccgc ctgccccgac gccgccacca gcggcaccac ccacggcgca
     6541 agccgcgcct gcgcctgcag cggcacccgc ggcacgcccc gccgcgccgg tgcagatcga
     6601 tgaagcgggt ttctcgcgcg cgcatgcggg gccgtcggta cgcaagctgg cgcgcgagct
     6661 cggtgtcgat ctcgtgcagg tgcgcggcag cggcctgaag gaccgcatca ccgcggacga
     6721 cgtcaaggcg cacgtgaagc aggcactcgg cgcaattgcc ggcgccggca ccgcggcaca
     6781 aggtggcggg gcgctgccgg ccgtgccgag cgtggatttc gcgcaattcg gtccggtcga
     6841 ggtcaagccc ctggggcgca tccagaagat ttccggtgcg cgcgtgcatg cctcgtgggt
     6901 gaacctgccg cacgtcacgc agttcgacga ggccgacatc accgacctcg aggccacgcg
     6961 cgcgaagctg aagggcaagc tcgagaaatc cggcgtcaag ctcacgctgc tcgcgttcat
     7021 catgcgcgcc tgcgttcgaa ccttgaagga gttcccgcag ttcaacgcct cgctcgatgc
     7081 cacgcgcgcg aacctcgtct tcaagaaata ttttcacatc ggttttgcgg cggatacgcc
     7141 gaatggtctg ctcgtcccgg tggtgcgcga cgccgaccgc aaggatgtct acgagatcgc
     7201 gcgggcgctg gccgagctct cggcgcaggc gcgtgcgggc aagctgcccc cctcggatat
     7261 gcagggcggc agcttcacga tatcgagcct cggcggcatc ggcggtacgg ccttcacgcc
     7321 gatcatcaac gcgcccgagg tggcgatact cggcgtgtcc cgttcgatca cgaagccggt
     7381 gtaccgggac ggcggcttcg tgccgcgcct gatggtcccg ctgtcgctct cctacgatca
     7441 tcgcgtgatc gacggggcgc tcgccgcgcg cttcacgacc cggctggccg aacatctcgc
     7501 cgatccgcag ggcctgctcg aggccgttcc gtgagcatcc gcgatgtcct ggtccccgat
     7561 ctcggggagt tcaaggatgt gccggtgatc gacgtgctcg ccgcgcccgg cgatcgcctc
     7621 gaggtcgaca caccactgat cactctcgag accgacaagg cgaccatgga cgtgccgagt
     7681 cccgtggccg gcaccctggt cgagctgacc gtgcgcaagg gcgagcgcgt ctccgccggt
     7741 gtgctgatcg cgcgggtgga gacggctccg gaagatcgtg ccggtgcgag cggcagcgca
     7801 ggcgcgcaga ccgccgcggc ctccccggcc acggacagcg attcgctgaa aacgaaggtg
     7861 ctgctgcagc tcgcgggcgt gcccgaggag atcgaaacgg accgcagcgt gcagttgctg
     7921 gtgctcggcg cgggccccgg tggctacacc gcggcgtttc gcgccgctga cctcggcctc
     7981 gcggtcacgc tcgtcgatcg ctggccaagg ctcggcggcg tgtgcctcaa cgtcggctgt
     8041 attccgtcga aggccctgct gcacgccgcc agggtcatcg aagaagcgca tgcgatgggc
     8101 gcccacggcg tcgagttcgg cgccccacgc atcgacaagg acaggctgcg cgactggaag
     8161 tcgggtgtcg tgaagcgcct gacgggtggg ctgtcgatgc tcgcgaagca gcgcaaggtc
     8221 gacgtggtgc gcggcagtgg ccgcttcctc ggtccgcacg tggtcgaggt gacccgcgaa
     8281 gacggcggca gcgagcgcat ccgcttcgag caatgcatcg tcgcggcggg ctcggagccg
     8341 atgcgactgc cgttcgcgcc gcacgatccg cgcgtcatgg actcgaccgg cgcgctcgag
     8401 ctcaccgaca tcccgcagcg cctgctcgtg gtcggcggcg gcatcatcgg cctggaaatg
     8461 gcctgcgtct acgacgcgct cggctcgaaa gtcagcgtgg tcgaactgac gaagcagctg
     8521 atgcccggct gcgatcctga tctcgtgcgg ccactcgaga agcgcctgcg ggcgcgctac
     8581 gagcgcatcc tgaccggcac gaaggtcacc gccatggcgg cggaggcgga tggcatccgc
     8641 gtcagcttcg agggcgcgga cggtgcgcag cctgcgacct acgatcgggt actgatcgcg
     8701 gtgggccggg tgccgaacgg cggccggctc gaggccgagg ccgccggtat cgccgtcgat
     8761 gaccgtggct ttatcgcggt cgacaagcag atgcgcacca atgtgccgca cattttcgcg
     8821 atcggcgaca tcgtcggcca gcccatgctg gcgcacaagg cgatgcacga ggcaaaggtc
     8881 gccgcggagg tggccgccgg cgggaaacac gccttcgatg cgcgggtgat tccatcggtc
     8941 gcctataccg atcccgaagt cgcctgggtg ggcctcaccg agaccgaggc gcgcacgcgc
     9001 ggcatcccgg tcgccaaggg cgcgtttccc tgggtcgcga gcgggcgctc actctccatg
     9061 gcgcgcgacg agggtttcac gaagctgctg ttcgatccgc agacgcgccg tgtgctcggc
     9121 gccgggatcg tcggcaccaa cgccggagag ctgatcgccg aactcgcgct cgcgatcgag
     9181 atgggcgcgg acgcggccga tatcgcgctc accgtgcatc cgcatccgac gctgtcggag
     9241 accgtcgggc tcgcggccga ggcgttcgag gggaccctga ccgacctcta cgtgccgaag
     9301 aagtcgcagt aacgcgcaat cacgcagtcc gcgcagcgcg gtttgcgtgc cgtgcacacg
     9361 tagcgcccat gcaggatcag ccagtggtgg gcatcgcgcg caaattcagc cggtgtggtc
     9421 gcgacgagga tgtcctcgac ctgccgcggc gtgcgtccga cggcgagacc ggtgcggttg
     9481 gcgacgcgaa agatgtgcgt gtccacggcg atcgtcggct cgccgaacgc cgtgttcagg
     9541 atcacgttgg cagtcttgcg gccgacaccg ggcagcgcct cgagcgccgc ccggtcatgc
     9601 ggtacctcgc caccgtggcg ctcgatgagg atccggcagg tctcgatgat gtgcttcgcc
     9661 ttcgagttgt agaggccgat cgtgcggaca taatcgctga gcccgctggc gccgagggcg
     9721 aggatcgctg ccggcgtgtt cgccaccggg aacagcttgc gggtggccgc gttgacgctg
     9781 cggtcggtgg cctgggcgga gagcaccacg gccaccagca gttcgaacgg cgtcgagtat
     9841 tcgagctcgg tcgtcgggtg ggggttggcc tggcgcagtc gcacgaagat cgcgcgccgg
     9901 cgctgccgcg tcatcgggcg ccgtccggcg ccgctcatcc agcgaggccc ttgcgggcgg
     9961 cgagcagcgc gtcgcgctcg gcggcgcggc gcgccacgcg cgcattgcgc agcgcgtagc
    10021 gcagccggtt gtccgccgcg gcaggcggcc gcggcgaact gacgggccgc tccaccatcc
    10081 ggatgcagtc gaccgggcac ggcgcgacgc acaactcgca gccggtgcac agatccgcga
    10141 cgatcgtgtg catgtggcgg gcggcgccga cgatggcgtc gaccgggcag ggtggcaggc
    10201 atttcgcgca gccgatgcag agtgcctcgt ctatcaccgc gacgcgctcg ggctgctcgc
    10261 tgccgttggc gggattgagc ggcagcggct cgcggccgag caactgcgcg agtgccgcga
    10321 tggtgcgcgc gccaccgggc gggcactggt tgatcggcgc ggcgcccgag gcgatcgcct
    10381 cggcgtacgg acggcagccg tcgtagccgc agcgggtgca ctgcgtctgc ggcagcagcg
    10441 catcgatggc gtcggcgcgt gattcagcca gcgtcacgtc actttcatgc cggggacggc
    10501 accgtcatcc ggggcgagca ggtagatgcc ctgcgaatca tcggccccgc tcgcgcacag
    10561 caccatgcct tccgaggtgc cgaagcgcat cttgcggggc gcgaggttgg cgaccatcag
    10621 cacgaggcga ccttcgagcc gggcgggctc ataggcggcg cgtatgccgg agaaaaccgt
    10681 gcggcgctcg ccgccgaggt cgagctcgag gcgcagcagc ttgtcggacc cctcgacggt
    10741 cgaggcggcg atcactctcg cgacgcgcag gtcgagcctg gcgaagtcct tgatgtcgac
    10801 agtggcggcg gcgggcgccc ttgcggccgc ggcgggtgcg ctcgcggcga cggcgggtgc
    10861 gcccgcggtg gcggccggcg cctgtgcctc ggcgcccgcg tcgatgagcg ccgccacgct
    10921 gctgcgatcg agtcgcgtcg cgagcggctt gaactcgttg attgcgacat ccagcagggg
    10981 cgtggcgatc gcgtcccagc gatcgatcgg ggtgccgagg aatgttcccg cgtccagcgc
    11041 gatgcgcggc aggaccggcg cgagataggt catcagcacg cggaacaggt tgatgccctg
    11101 cgtcgcgacc gcgagcacct cggcggcgcg cgccgggtct ttggccagta tccacggctt
    11161 gtggtgatcg acgtactggt tggcgcgatc ggcgagcagc atcacttcgc gcacggccgc
    11221 ggagtagtcg cgcgcgtcga acagcgcggc gatgcgtccg cgcgcgtcgg cgaactcctg
    11281 gtacagcgcc ggctcgggca gcctggcggc gagtctgccg cccccacgcg cgatgaagcc
    11341 ggcgcagcgg ctcgcgatgt tgaccagttt gccgacgatg tcggagttca cgcgcgcgac
    11401 gaggtcatcg aggttgaggt cgatgtcgtc gaggtccggg ccgagcttgg ccgcgaagta
    11461 gtagcgcagc ggttcggccg gcaggtgctc gagatagcgc tgcgcggtga tgaatgtgcc
    11521 gcgggacttc gacatcttct ggccgttgac ggtcaggaag ccgtgtacgt agacggccgt
    11581 cggcttgcga aaacccgagc cgtgcagtac cgccggccag aacagcgtgt ggaaatagct
    11641 gatgtccttg ccgatgaaat gatggagttc cgcgctgctg tccggccgca tgtattcgtc
    11701 gaagctgcgg ccagtccggt cgcagagcgc gcgcaggctc gcgaggtagc cgatcggcgc
    11761 atcgaaccac acgtagaaga acttgccact ctcgtcggga atctcgaagc cgaagtacgg
    11821 gtggtcgcgc gagatgtccc agtccttgag cccggcgtcg aaccactcgg cgagcttggc
    11881 gcgcacgccg ggctgcagcg cgccggtggt gagccattcg cgcagcattt cctcgaacgc
    11941 gccgaggcgg aagaagatgt ggtccgattc gcgccacacg ggcgtcgtgc ccgagatcgt
    12001 cgagaccgga ttgacgaggt cggccggcga ataagtggcg ccgcagtgct cgcaggagtc
    12061 gccgtactgc tctggcgtac cgcaccgcgg gcaaccaccc ctgacatagc ggtcgggcag
    12121 gaacatgcct gcctgctcgt cgtacgcctg gcgcaccggc cggcgcgcaa tgtgcccggc
    12181 gtcgcgcagg cgcaggtaca tcgactcggc gagcgcccga ttctcgggcg aatgcgtgct
    12241 gtggaactgg tcgtgcccga tcaggaaacc ggcgtagtcg cgagcgtgtt ccgccgcgac
    12301 gcgctcgatc agcgcctcgg gcgtcacccc ctcgccctgc gcgcggatca tgatcggcgt
    12361 gccgtgcgca tcttcggcgc agacatagag gcagtcgtgg ccgagcatct tctgcaggcg
    12421 gacccagatg tcggtctgca ccgcttcgat gatgtggccg aggtgtagcg gcccgttggc
    12481 gtagggcagg gcgctggtga cgaggattcg acgcatccgc gcagtgtacc ggagagcccc
    12541 tgcggccggt gcgcaggaag gggcccggat cgcaggccgg gcgctagccc tggtccttga
    12601 ccgggtcgaa cttcgccttg ttgatgccct tcttgtaggc ctctttgcca gtgatcgcgc
    12661 gctcgcgcaa cagttgttcg atcgcgctgt ccatggtgcg catgccggcc tgcgcgccgg
    12721 actgcatcac cgtctcgagc tggtcgagct tgccctggcg gatcaggttc gagaccgccg
    12781 aagtattgac caggatctcg agcgccgcga cgcgaccctg cttgtcggcc cgttgcagca
    12841 gctgctggga gattacgccc cgcagcgaag tcgagagcat ggtgcgcacg tgcgactgct
    12901 tgtcggacgg aaacgcattg accatgcgat ccaccgtcgg agctgcgccg ttcgtgtgca
    12961 gcgtacccat cacgaggatg cccatctcgg ccgccgtgac cgcgatgctc atcgtctcga
    13021 ggtcacgcaa ctcgccgacc aggatcacgt cgggatcctc gcgcagggcc gagtgcagcg
    13081 cggcggcgaa gctcgggctg tgcacgccga tctcgcgctg gctgatcagg cagttcttgc
    13141 gctcgtgcac gaattcgatc gggtcttcga cggtgaggat gtggcccttc tcgcggcggt
    13201 tgatgtcgtc gatcatcgcc gcgagggtgg tcgacttgcc ggagccggtc ttgccggtca
    13261 cgaggatcag gccgttgttg gcgcggcaca gctgccgcac ggcctggggc agctgcagct
    13321 cgtcgagcgt cagcgccttg gaggggatcg cgcggaacac cgcgcccatg ccgttcaggt
    13381 ggcgcagcac gttgacgcgg aagcgtccgc ggcccggcag cgtgtaggcg aagtccgcgc
    13441 cgtccttcgc ctcgaagcgc tcgaccgcct ggcgcggcat gatttcgtac agcgtttcct
    13501 tgaccgccgc agcgtcgagc cgctccggac gcagcggcga aagatcgccg aacagccgga
    13561 tgcgcggcgg atcgccggcg aggaagtgca ggtcggagcc cttgcgatcg aggatttccg
    13621 cgagcagtgc gtcgatgcgt gccatggggc gcccgtcagc cgccgccggt cgcgccgctc
    13681 gccggcgccg gggagtcgag tttcggcagg atgctcgtgt cggtcacgaa cttctggaac
    13741 aggcgcttgt cggtcgcgta gttgtaggcg tcgtcgggat cgatttcctt ggcgttgacc
    13801 gccgcaagca gcgcctggtc gagcagctgc atgccctggt cacgtccggt ctgcagctgg
    13861 ctcggcagct ggtgcgtctg gtcgctctgc acgagcttgg cgatcgcctt ggtcatgacc
    13921 atcacttcgc agaccgcgcg gcgggcgcgg ccgtcgacgc tcttgacgag cacctgggtc
    13981 acgaccgcga gcaggctctg cgcgaggaag ctcttggtct gctcgcgctc ctcgaccggc
    14041 agcgcatcga tcacgcggtc gatcgtcttg gtcgcgctgg tcgtgtgcag ggtgccgagc
    14101 accaggtggc cggtctccgc ggcggtcatc gccatcgaga tcgtttcggc gtcacgcagc
    14161 tcgccgacca ggatcacgtc ggggtcttcg cgcatcgcgg cgcgcacgcc ctcggcaaac
    14221 gaggggatgt gggtgccgag ctcgcgctgg atcacctggc tctgcttgct gcggtggacg
    14281 aactcgatcg ggtcctcgag gctgatgatg ttcagtctgc ggttgatgtt cagcaggtcg
    14341 atcatcgccg cgagcgtggt cgacttgccg gtgccggtcg agccggtcac gaggatcatg
    14401 ccctggtgga aatcgcacag tttcttgacg atcggcggca ggttgagcga atcgatcgtc
    14461 gggatctcgg tcgggatggc gcgaaacgcc gcgccgacgc cggtgtcctt gcggtacacg
    14521 ttgacgcgga aacgcccgcc ctcgcccgaa acgtaggaaa agtcgaggtc gtggccctgc
    14581 gcgaaatgct cggcctggga cctggtgagg atctcggtga tgtagccctc gagttcggtg
    14641 tcgcgcagct cgcggaactt gatcggcatc aggtcgccat gcatgcgcag catgggcggc
    14701 acgcccacgg cgaggtgcag gtccgagcaa ccctgctgca tgccgagttt gaggaacgcg
    14761 tcgatgcgcg ccacgaagtc ctcctggtcc gagccgactc ttgctagccg cttgaatagt
    14821 ctgaaaaggc tgtcagatca atggctaacg ctgaaaacct tgaccgagtg cacgaagtct
    14881 acagcggtca caggaaacgc gacagggcgg ccttgaactc gtccatggtc tggtagcgct
    14941 tcgtcttgtc gacggccatc gccttcatga cgacttccga aaggccctcc ggcaaggacg
    15001 ggttcacgtc ctttacggtg cgcgccttgc cctgcacgtg ctggtacatc accgccatgt
    15061 gatcgccgcg cgagtagggt ggcacgccgg tcagcatctc gtagaggatc acgccgagcg
    15121 catagacgtc ggcgcgctgg tcgaccttct tgccgaggat ctgctcgggc gccatgtact
    15181 tcggcgagcc gatcacgtag ccggtcttgg tcagctgcgt gtcgccctcg cgctgcgcgg
    15241 cggcgacgcc gaagtcgacg atcttgagca gcccctcgtt gttgatcagc aggtttgcgg
    15301 gcttcaggtc gcggtgcacg acgccgacct ggtgcgcgac cgccatgccc gtgcagatgt
    15361 cgatcgcgaa gcccagcgcg cgcttcagct ccatcggctt ctcgccgacg atctcggcgc
    15421 cgagcgtgtg cgaggggaag tactccatcg aaatggcgta gtgcccctgt atgtacagga
    15481 agtcgtagat gcggatgacg ttctggtgcg tgatcttgcg cgagtaacgc agctcgtgca
    15541 cgaagcgctt catgatctcc tcgtcctgcg ccacgttcgg attcaggaac ttgaggatca
    15601 gccgctcgtc gaccacggtg tcttccatca gcagcacggt gccgaatgcg ccgcggccga
    15661 tgcgctcgat gtacttgtag cgtccctcga tcacgtcgcc cggattgagc gtcgagatat
    15721 cgagcttcgg ctgcgtgctc gcctcggcct ggcgcacgac ctgcgccact tcggcctccg
    15781 acatcagcag cgtgcgtgcc gcctccgtcg ggcgctgcgg ggcgtgcccg acggtgcccg
    15841 gcaccggcgt ggccgcgagc gtgccgcggc cgctgccact gctgctgagc atgctgccaa
    15901 agcgggcgtc gagttccgtg agcgcatgct gcgcggcctg cgcgatcgtc ggatcgggca
    15961 gcgcggtctg cgcctgcaaa tgcacgcgga tggcttcggc gcggcgttca tcggcgagct
    16021 tcgcgagggt gcccatcgcc tcgacgcgga tctccttctc gggacgcgcg aggaacggca
    16081 gcagcgtctc gatcactttg tggtcgccga gcttggcgag cgcgcggatc gccaccggga
    16141 tcgagcgcgc cggcgccgtc tgcagcagct cgacgatgcg cggcaccgcg cgcttgctgc
    16201 cgatttcggc cagcgcgtcg accgcccgct cgctgaccca ccagtccttg tcgcgcgtcg
    16261 cctcgatcag gtgcgccacc gcgcgctcgt ccttggtctg gttgaggatc tcgatcgcgg
    16321 cgcggcggat atcctcgtcg tcgtccttga tcaactgcag cacggcgtcg accaccctgg
    16381 gcccgccgat cttgccgagc gcatcggccg cgcggctgcg cacccaccag tcgtcgtcct
    16441 tgatggcgtc cagcaggaac ttgacggact tcgcggtgcc gacttcgttc agcacctcga
    16501 ccgcggcgcg tcgcgcgaat tcgttctcgt ccttcagcac cggcacgagg tacttgatgg
    16561 tctccgggtc gttcgccttg atcacgacgt cgatcgcctt gttctgcacg tcgatctcgg
    16621 ggtcgcgcag catctcgcag acgcgctgca cgtcgatcgg gccgtccatc ttgtgcagtg
    16681 ccgcgagtgc cgcggcccgc accagcttgt tgttgtcctt cagctggtcc tgcaatgcgc
    16741 tctgcacggc gggcgtgttg aagcgcgaga ggatattcag caggtgcagc cgggcgatcg
    16801 ggtccttgcc ctgcaggcgg ccgatgagct ccgggagcgc gctcgcgtcg gccatgtcgt
    16861 tgatgatgcg aaagagcgca gccttctcgt tcggctcctg cgcgtaggcg gcgttcagga
    16921 gctcgcgcac gctgaagcgc tgcttttgcg tcgcgatgat gtcgaccagc gccgacttcg
    16981 agatgccctc gagctgcagc gcctcgagca gcaggtgcgg cgggtaggca cggctgctgg
    17041 acagcgccca agagatgccc gcgacgaccc gcgggccacc ttcgaccagt ccgcgggtga
    17101 acaggtgaaa attcttcgca ttgacgatcg aggcgaggac gtcgacgaac acgaccgtgg
    17161 cgttcttgtc ggcgtccgcg agggcggcaa acagggactc gacggccgct ccgcccagcg
    17221 atttcagccg ggcggcagcc ttctgtgtct caggatcgtt cggatccttg gtcgcgatga
    17281 tctgcgtgat gaagcgatca gcccgcagat tcgagaaaaa actcattcag ccctttcccg
    17341 ggcggcttgt acccgcgctc tgcctgtgga ccgacggaac ccccaccatc ggtgaagtat
    17401 gccatatgga cccatgcgag catcgtgcca gcggtcggca cctccggcaa ttttcgctag
    17461 aatccaccca cacgtgccga ccggcgcccg ggaatccctg atgcctaaca accaggacgc
    17521 tgcgcttctc gcagcgcttg ccactttcgt cgacccctat gcgggtgaaa cgcttgcgac
    17581 gctgcaggcg gtcgagcacg ccaccgtcgc ggcgggccgc gcaaacgtgc gcttgcggta
    17641 cggcttcccg gtcggcggct atgcgcacga actgcagccg ctgctcgccg ggcacctggc
    17701 tgccgcgggc atcgcattgc cgctcgagct cgaactcgcg gcggacattc gcgcgcatgc
    17761 cgtgcagcgc cagctgaagc caatggcgtc ggtcagcaac gtggtggcgg tggcctcggg
    17821 caaaggcggc gtcggcaagt ccacggtggc cgcgaacctc gcgctcgcct gggccgtgca
    17881 gggcgcgcgc gtcggcatcc tcgatgccga tatctacgga cccagccagc cgcagatgct
    17941 cgggcttgcc ggtgagcgcc cggaaatcgc gccgggcaag cgtatccgcg cactcgaggc
    18001 gcacggcatc gtggccatgt cgatcggctt cctgatcgat gcggcgcaac ccatggtctg
    18061 gcgcggcccg atggtgacgc aggcgctgac gcaactgctg aacgacacgc tgtggggtca
    18121 gctcgactac ctcgtggtcg acatgccgcc cggcaccgga gacatccagc tgacactcgc
    18181 gcagcgcgtg ccggtggcgg gcgcggtcat cgtgacgacg ccgcagcata tcgccgtagc
    18241 cgatgcgcgc aagggcctca ggatgttcga gaaggtgtcg gtgccggtgc tcggcatcgt
    18301 cgagaacatg agtctgcacg tgtgctcgaa ctgcggccat gccgaggcga tcttcggcga
    18361 aggtggcggt gacgagatgg cgcgcgagta tggcgtcgcg ttgctcgggc gcctgccgct
    18421 cgatgcgcgc atccgcgaag aggcggacgg cggccggccc agcgtgatcg ccgcgccgga
    18481 ttcgccgcgc gccgcggctt acctcaccat ggcgcgccgt accgccgccg tgctcgcgcg
    18541 tcgcgagcgc gatcgcagca ccaccttccc gaacatcgtg gtcgaggaga cctgaataga
    18601 tgagcatcaa ggccgacaag tggatccgtc gcatggcttg cgggcagaag atgatcgagc
    18661 cgttcgagcc agggcaggtg cgcgaggtcg acggccggag aatcgtctcc tacggcacgt
    18721 cgagctacgg ctacgacgtg cgctgttcga atgagttcaa ggtgttcacg aacatcaact
    18781 cgaccatcgt cgatccgaaa ggcttcgacg ccaagagttt cgtcgacatg caggccgatg
    18841 tctgcatcat tccgccgaat tcgttcgcgc tggcgcgcac ggtcgagtat ttccgcatcc
    18901 cgcgcaacac gctggtcgtg tgtctcggaa agtcgacata cgcacgctgc ggcatcatcg
    18961 tgaacgtgac gcccctcgaa ccggagtggg agggccacgt gacgctcgag ttctcgaaca
    19021 cgacgccgct gccggcgaag atctacgcga acgagggcgt cgcgcagatg ctgttcttcg
    19081 agtcggacga ggtctgcgaa acctcctaca aggaccgcgg cggcaagtac cagggccagc
    19141 gcggcgtcac gctgccgaag acctagagcg cctcgtcgcg ccgcagttcg cggatcgcca
    19201 tcgcgaactc gatcttgtcg gcgcgaaaac gctcggcgcg caccggcagg tagtcgagcg
    19261 agggcgccat ccacaggcgg atcatgcgtt tcgagccgcg ccggcggctc tcgtagatca
    19321 cggtctcgag ttcgcccagc gaggtcagga tcttctgcgt gccgctgcgg gtgaagtcgt
    19381 attccttgat ctcatccttg tcgacggtgt ggaaggccac ggcggtgacg ccggcggcgg
    19441 cggcgcgcat ctgcgcgagc tgtacggtca tcgagtcgtg gatgtcgggc ggcatcgcca
    19501 gatcgacggg ctggttttcc gcggtgccgg tgatgcgcat gtgctgccag tcgaagtcga
    19561 gcgcgacgtc gcgatcggtc gcgtcgccgc cgtcgtcgcc gcggaaccgg agcggctgcg
    19621 gctcgccggc gacgaatcgc agttccgaat cctgctcgac cgtgtccggg atggcgatgc
    19681 ggaagatgcc ccgcgcgttg ctgcgcgaga cgtacgccca ggtgtcgacg ccagtgcgac
    19741 gcagttcgag ggtcgaggtg cccgcgttca ggcctttcca ttgcaccgca taggtggcgt
    19801 ggaagggtgt ggggtgccag tcggcggcca ggctcgcgct cgcgccggcg agcgcgaggg
    19861 ccgccatggc caggctcgcc agggcgctcg ccctccacag tcgcgtgtca gccggtcgcg
    19921 ggtccatcga aatcctccgt gatcggagcg gtcagcagtt tgtcgtcgag ccagacgcga
    19981 tcgtcgcgcc acgccagccg tccggctgca atccagccga tcgcacgtgg atacacgata
    20041 tgttccagcg cctgaacacg ggctgaaagg cgctgctcgt cctcgcccgg cgcgacgcgc
    20101 agccgcccct gcagcacgac cggtccgcca tcgagctcct cggtgacaaa gtgcaccgaa
    20161 cagccgtgca tggggtcgcc ggccgcgagt gcccgccggt gggtatgcag acccttgtac
    20221 tttggcagca gcgaggggtg gatattcagc atgcgcccgc ggtaacgggc gacgaacgca
    20281 tccccgagaa tgcgcatgaa cccggccagc acgaccagat ccggacggca ctcgtcgagg
    20341 cgcgcggcga gggcggtctc gaactccggc cgcgaggcat acgccgccgg cagcacggca
    20401 aaggcatcga tacccagcgc gcgcgcggtg tcgagtcccg cggcgtcggc acgatccgtg
    20461 ccgacacagg caacgcgcgc gttgatcgtg cgcgtggcgc aggcccgtgc gatggccgcc
    20521 atgttgctgc cgcggccgga gatcaggatc gcgagccgca gcggtggcgg cgcggtcatg
    20581 cctcgatcac gacaccggca gtgccgccgc gcacggtccc gatcacgctc gccacttcgc
    20641 cgtgggcgcg cagcagcgcc agcgaggcat cgacatcggc ggcggcgacg atcaccacca
    20701 tgccgatgcc gcaattgaac gtgcgatgca tctccgcgtc aggcaggctg ccggtgcgct
    20761 gcagccagtc gaacagcggg tcgcgatgcc acgtgcggcg cgacagcacc acctcgaggc
    20821 ccgcgggcag cgtgcgcggg atattgtcgg tgaggccgcc gcccgtgatg tgcgcgaggc
    20881 cgtgcaccgg gatgcgccgc gccagctcga gcagcgagcg cacgtagatg cgcgtcggcg
    20941 cgagcaggcg gtcgaacagc ggctggccgt cgagctgcgt gccggcattc gcgccggcga
    21001 cgccgatcag cttgcggatc agcgaatagc cgttcgagtg cgcgccggag gaggcgagtc
    21061 cgatgaccgc gtcgcccgtg gtgacccggc tgccgtcgat gatggcgtcc ttctcgacga
    21121 tgccgacgca gaagcccgcg aggtcgtagt cgtcgcccgc atacatgccc ggcatttcgg
    21181 ccgtttcgcc gccgaccagc gcggcaccgg ccagcgcgca gccggccgcg atgccgctga
    21241 tcacggtggc ggcgacgtcg acatccagct tgccggtcgc gtagtaatcg aggaagtaga
    21301 gcggctcggc gccctgcacc gcgacgtcgt tcgcgcacat cgccacgagg tcgatgccga
    21361 tcgtctcgtg gcggccggtt tcgatcgcga ggcgcagctt cgtgcccacg ccgtcggtgc
    21421 cggaaacgag caccgggcgc acatagcgat cgagcggcac ttcgacgagt gcgccgaagc
    21481 cgccgatgcc gccgagcacc tcgcggcgct gcgtgcggcg caccagcggc ttgatgcgtt
    21541 cgacgagggc gtcaccggcg tcgatatcga caccggcgtc ccggtaggtg atgggcggag
    21601 ggggcacagg catcccgcta taatagccga catgcatgcc gctatccgtt gcgtcgccct
    21661 tgcattgctc gccggcctgc cgactgccga ggcggcgcgc actgtgcgtg tctacgacgt
    21721 cgcggtgcgc gaagccgcgt ccgccgacgt gccggccgcg gcgatgcgaa ccgcgctcgt
    21781 gcgcgcgacc ggacaacgag cggcggcgtc cgatccggcc ctggccatgc tcgtcgccga
    21841 cgcgccgcgc tatgtgaggc tgtctcgccc gggcgccgac ggacgcaccg aggtctcgct
    21901 cgatggcgac gccatcgact cggcgatcct ggcggccggc agggtgctgt ggagcgccga
    21961 gcggccactg accctggtgg tactcgaagt ggctccgggc gcggtcatcg acgcgggggt
    22021 gcaggcagcg atcgaggcgg cggcaggtga tcgg
//
LOCUS       DAOQVY010000033        21287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_2903_length_21287_cov_6.298182, whole genome shotgun sequence.
ACCESSION   DAOQVY010000033 DAOQVY010000000
VERSION     DAOQVY010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 21287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21287
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2903_length_21287_cov_6.298182"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /gene="argS"
                     /locus_tag="PKN91_04865"
     CDS             complement(<1..1069)
                     /gene="argS"
                     /locus_tag="PKN91_04865"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNS27439.1"
                     /translation="MKTELETLLAAALARLTEAGSLPPADPAAIVVDRTRDARHGDFA
                     SNVALRLAKVAGRPPRELAAEIIAALPANRLVARTEIAGAGFINFHLDANANLDELWR
                     ALELGAKYGLSTTLGAGRKVILEFVSANPTGPVHVGTGRQAAFGATLGNLLEATGHVV
                     HREYYINDAGRQMDILTASVWLRYLETCGEALEFPSNSYRGDYVRVLGAALHAAQQEA
                     FRRPASDVFSGLPPDEPAGGDKDKYVDAIIERMRALLGASDYQRVLDFSLAAMLADIR
                     NDLADFGVVFDRWYSERTLATSGAIDHALDVLRGQGRLYEKDGATWFRATEFGDDEDR
                     VVVRANGEKTYFASDIAYHLEK"
     gene            1180..1680
                     /locus_tag="PKN91_04870"
     CDS             1180..1680
                     /locus_tag="PKN91_04870"
                     /inference="COORDINATES: protein motif:HMM:NF024890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HNS27440.1"
                     /translation="MKSTVIVLALMAAPLAVLADDTEPGRGHMGGYQMGGKNLIQTLD
                     ANKDGKVSRDESSKAAVERANRRFDQLDADKDGFITQAEIDAARDKMRSEVRGEMSRR
                     GAENWKAADKDGDGAISRSEAEAGMPMLFRRFDQLDANKDGKISRDEMPQGKRMDDGR
                     KGPPTS"
     gene            1844..2944
                     /locus_tag="PKN91_04875"
     CDS             1844..2944
                     /locus_tag="PKN91_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNS27441.1"
                     /translation="MSNRLSWLSGALVAATLILTAGASAAEPVPAAGRKVVLDLAGDR
                     FQWKIVEAPVAAPGPHQVLMRVRAVALNRGDVEVLDPKFGAKAGLVVASDAAGEIVAV
                     GSEVRDFRVGERVNTLYFRNWTDGPPSGEKLAATHGATVDGVLADYLVLDDTAITAMP
                     NGLSFEEAATLPTAGLTAWMAVNGHRALRAGDVVVVQGTGGVSTLALQLATALGGRVI
                     ATSSSDGKLARARALGAEGGINYRTTPAWSARVLELTGGHGADVVVDVVGKAALPESL
                     QSLAYGGTLSLVGGLTGFDGQVPAIQLLLKTARVQGICVGSRADYRRMAEFIGQREFH
                     PAIDRVFPLEEYAAALAHMESGDFVGKIVLTL"
     gene            complement(2958..5228)
                     /locus_tag="PKN91_04880"
     CDS             complement(2958..5228)
                     /locus_tag="PKN91_04880"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004067.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNS27442.1"
                     /translation="MTERFGTRRRSFNQLVVLQRVFLVGLSIIRVAVDTPLRRTFDYL
                     AGEEVPRPGARVRVPFGRRQIIGIALEIGVDSALPPGKLKPVAAVLDAEPVFDEGLLA
                     LLRWAADYYHHPIGEVLFSALPKLAREGARTRAVREVWSPTTEGIAAHTAGEPRRAPR
                     QRAILEELIERGSLDATALAALGTGAREALTALTRRGWACARDLADEAAPAETGAGAI
                     ANGSPFELTGEQQAAVDAIEAGHGRFGAFLLQGITGSGKTEVYLRLTERALARGERAL
                     ILVPEIGLTPQLIGRFAARFAVAMATMHSGLTGTARLNAWRDALSGRARIVLGTRSAV
                     FAPLPGLGLIVVDEEHDPSFKQQEGGFRYSARDIAVMRAQRAGVPIVLGSATPSLETL
                     HNAQAGRYTRLALSRRTGEALPPRLTLVDLRAEALRHGLSTTVVEAIRRHLDDGGQVL
                     VFLNRRGYAPTLACTACGWIAGCSSCDARLTVYARASRLKCHHCGADAALPTRCPQCG
                     YEVRAVGQGTERVEDTLAELFPHVALARLDRDVVRRDEQLAAVLARVASGEARILVGT
                     QMVTKGHDFPDVTLAVVLNADHGLFSTDFRAAERLAQTIVQVAGRAGRGARAGEVLIQ
                     TEYPEHPLLASLLSEGYEGFAATALAERQVACWPPFGRLAAVRASALTLHAAVEFLGE
                     ARRLLAPPRQVKLLGPAPAAMARRADRFHAQLLVESSDRAALHRYLDAWLPLAEALPS
                     ARRVRWALDVDPLELF"
     gene            5308..5892
                     /locus_tag="PKN91_04885"
     CDS             5308..5892
                     /locus_tag="PKN91_04885"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNS27443.1"
                     /translation="MSFAHGDGHDMPDALRARNAAFDRFFIAVERKAFRIARLALQND
                     DDALDAVQDSMLQLVRAYASRPQAEWKPLFYRILANRTRDLQRRRSTRGRLFAWWPTG
                     GETDDGDDPASAAPSPEPGPERSLELGQTMALLDQFIGQLSHKQQQAFLLRNFEDLDV
                     AQAARAMGCSEGSVKTHYSRAVHRLRARLMEAES"
     gene            5889..6227
                     /locus_tag="PKN91_04890"
     CDS             5889..6227
                     /locus_tag="PKN91_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27444.1"
                     /translation="MTTGREIERQARETLEASIEHLDARVRSRLTRARHAALDELQRR
                     ARRPAWFVVPAVGFAASAILAVVLWAPWSTVQDPANGTALTALATPDADLDFLLGEDV
                     FAAVFEDPAG"
     gene            complement(6261..6872)
                     /locus_tag="PKN91_04895"
     CDS             complement(6261..6872)
                     /locus_tag="PKN91_04895"
                     /inference="COORDINATES: protein motif:HMM:NF018228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS27445.1"
                     /translation="MNSEAVVFVVDDDAAIRDALSLLISLHGLRAQIFASAESFLDTC
                     RSEWRGCVLVDLQMPGMSGLDLQAELKARGIPLPVIVLTAHGDVATARAALKNGAVDF
                     LEKPVDDAVLIDVLRNAIELDAERSAAAQARAAAAERLARLTSRERDVLRLLAQGNQL
                     REIAERLEISPRTVEVYKARMMEKLGCRTLADVVRAGSELARE"
     gene            complement(6862..8442)
                     /locus_tag="PKN91_04900"
     CDS             complement(6862..8442)
                     /locus_tag="PKN91_04900"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HNS27446.1"
                     /translation="MPGSGHLLRAALFITAYVLIDWLSYLQPVLQLGITPWNPQTGVA
                     LAFLFWEGRRGAFAVAAAALLSEIVVRDVPAGSIAAITTSIWIAIAYAALAEVLRRKR
                     TSFLPSPGDAARFVGASLVATLLVGAGFIAIMLAMGALPGEVALGGVARYWVGDFNGV
                     LTLTPLLIWAPQWRAGLAALRAHWREALLQVAVLGATIWIIFGLLSDQALRFFYPLFV
                     PMIWIALRWGIVGAVVGALVIQIGLALMVTEAGMAAPLLDLQFLMLTLNLTALLLGAA
                     VSERQRAQEQLRERDAALARAMRFATAGEMASALTHELNQPITALVSYLRAAEMLAAQ
                     PDARLPETLDKAGREALRASEVLRRLRDFYSTGAAARAPLSLARMCEIVGAALHERLR
                     RARVQLVCSLDAGLPRVAADPTQLEIVLHNLVSNAIDAITLSADASGRIDITAAQRGD
                     DVLLSVVDSGPGVATEIAPRIFEPFVTDKPGGMGLGLAISRSLLRAQGGDLEYEPVAA
                     TGGARFTLRLKVAQADEQ"
     gene            complement(8596..9138)
                     /locus_tag="PKN91_04905"
     CDS             complement(8596..9138)
                     /locus_tag="PKN91_04905"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS27447.1"
                     /translation="MRRFLFPAPLLLAALALAACSTPLYRPSSGTTPSQPGTTTPEQP
                     GSAVPEPTPPATPAPVPPPAPPPRSHRLSPATNALVVQARTATSKGDFDAAAATLERA
                     LRIEPDNPLVWIEMGQMRLASGDPVQAENMGRRALALAAGAPREQASAWQLIGFALRA
                     RGRNVEAQEALQRAGALVAP"
     gene            complement(9138..11426)
                     /gene="mrcB"
                     /locus_tag="PKN91_04910"
     CDS             complement(9138..11426)
                     /gene="mrcB"
                     /locus_tag="PKN91_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HNS27448.1"
                     /translation="MKRRTKNILKNAGIAVGALAGAVAVAFVIALFYYDRQVAAQFEG
                     RRWTLPAKVYAAPLELYAGLAFSTDELIAELDRLQYRETNTLTRPGTWRRRRGRFEIA
                     VREARFSDELRPAQVIALTLERGVIASLRDARGNDIPIFRLDPLLIGSIFPIHGEDRI
                     VLTPEEVPQMLPAAIKAVEDRKFDAHHGLDFEAIARAFWVNLRAGRVEQGGSTLTQQL
                     VKSYFLDGRRTLGRKVEEAFMALALEARYDKRDLMTAYINEIYLGQQGDRAIHGFGLA
                     SQFWFAKPLDELDTAELALLVAIVRGPPYYDPRRHPGRAQQRRDLVLRILAQQQLISA
                     EEARKATAQPLGVAARPSGGYYPAYLDFVRRTLRRDYKEEDLTEAGLSIFTSFDPRAQ
                     VAAEQALESEIGRLDKRTRSDEGRPGLEGASIVTLPQSGEVIAMVGGRNVSFDGLNRA
                     LASARPIGSLVKPFVYLAAIETGRWNATSVLDDTPIEVPLSRNNVWRPQNFSRNANGP
                     VSMVRALSESLNLATVHLGLDVGVPKVAQMFTRFGLETEPRALPSLLLGAVDLPPIDV
                     AQMYNGLANGGFHVPLRAVRAVVNEQGERIGAYPVKVEPVAAPDAVYQIDRMLTYAIA
                     RGTGRAAAARLPRELIVAGKTGSSSDLRDGWFAGFSGTHLAVVWIGYDDNRPTGVTGS
                     SGSLIVWQKIMSALDTSSWQPPMPESLEDLPIDYASGLRVTTGCSQDEVIVAVPRGTS
                     PPFLAGCAPPSGIGDWLRGIIR"
     gene            complement(11436..13655)
                     /locus_tag="PKN91_04915"
     CDS             complement(11436..13655)
                     /locus_tag="PKN91_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNS27449.1"
                     /translation="MRQKSRWRRPVAALALLSVLITPIATARDRIGLVLGGGGARGAA
                     HVGVLRAIERERIPIDYIAGTSMGAIIGSLYASGYTVDEIQHILDTMDWRDVFRDGET
                     RDAMPMREKELDGAQLIPQEIGIGRDGVKLPPSIVVGQKFNIILRELLVQTEERISFD
                     SLPIPFRCVATDVGEAQEVVFSEGDLTAAVLASASIPGAFAPVRYDGRLLVDGMITNN
                     IPVDVARAMGADRLIVVNVSEPLLPEDKLGSPVSIMMQVLSGLVDRESARVLATLTER
                     DVELRPDMPDIASLDFAKVGEAVLAGEAAVAAHIGELRALAASPEEFAAHRATQARKP
                     LGNPHIAFVDVRSDTSRTAGLVRDRLGVQEDKRFDLEAVTDQIGRAFGGGTYERIDYR
                     LEERDGEFGLIVRPIDKAWGPTYLRMGLQFEDDFAGRDDYQFNAEVRMTGLSSTGAEW
                     RNLLQLGRESGITSEYYAPFGKLGRWFIAPQLQYIAMNQPTRLGDTEIARYRVDTGSG
                     ELRFGRDFGENLRLSLVASRFHDRGKLLVGDPSLPESMRADVAGIRAQFLYDSLDNVA
                     FPTRGLRLEASYGWLRAALGAHERGDLARLALDQAFTFGRNTVSIGARASVSTDSIAA
                     FSTMANLGGLTYLSGYGEDQLLGDQMVFGRLIYYRSFGSTRSLFRMPLYVGASFEAGN
                     VWEDFEDIAARDLIRAGSVFVGVPLPLGPLRFGFGYAEGGEQSFYLTFGAYVRPGFH"
     gene            13794..14294
                     /locus_tag="PKN91_04920"
     CDS             13794..14294
                     /locus_tag="PKN91_04920"
                     /inference="COORDINATES: protein motif:HMM:NF022338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNS27450.1"
                     /translation="MERDYQAPWGTMVRIGTWATLALLGAIAAMGLLIGPRQQLAWKL
                     AMIGAPLLLVASTLPFAIRGYTLTGRNLVIRRLGFDTTLALDDLRVVRGVADDVRGSL
                     RLWGNGGLFAITGWFWNRRLGRFRMFATDLSRAVLLEFARRKVLVTPHDPQAFIVHAR
                     KLMAMR"
     gene            14307..14516
                     /locus_tag="PKN91_04925"
     CDS             14307..14516
                     /locus_tag="PKN91_04925"
                     /inference="COORDINATES: protein motif:HMM:NF016028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HNS27451.1"
                     /translation="MADDRIELLETKLAHLELALQQLSDEMARQQKDIELLRERGRQL
                     ERQIEAAAQEAAGASATAIEIPPHY"
     gene            complement(14510..15142)
                     /locus_tag="PKN91_04930"
     CDS             complement(14510..15142)
                     /locus_tag="PKN91_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNS27452.1"
                     /translation="MTSQDRLVFFHSPNTRSTGTLALLEELGVPYDLQVIDMKGGEQR
                     RPEYLAVNPMGKVPAVRHGDALVTEQGAIFIYLADLYPAAGLAPPIGDPLRGPYLRWL
                     VFYGSAFEPAVVDRALKREPGPPATVPYGDYDTTIATLRGQLAQGPWLLGERFTAADV
                     LWGTALTWITAFGLVPADEPIKGYVERWNARPSVARARAIDEKLVASRAQ"
     gene            complement(15165..15500)
                     /locus_tag="PKN91_04935"
     CDS             complement(15165..15500)
                     /locus_tag="PKN91_04935"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNS27453.1"
                     /translation="MTTPVHERYPTACYIVICRDAPDGAEKRKGATADHLRHIEQVID
                     ELHVAGPLFDEAGKRPIGSVLLLRTQSERQARELTERDPYFRAGVWETIEVLPFFPAA
                     GGYIGGITW"
     gene            15634..16257
                     /locus_tag="PKN91_04940"
     CDS             15634..16257
                     /locus_tag="PKN91_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNS27454.1"
                     /translation="MIDLHTTPTANGLKASIMLEETGYEYRVHKIALTKREHLSPGYL
                     AINPVGRIPTIVDHDTASGNPLTVYGTAAILLYLAEKSGKLLPADLEARARVFQWLGI
                     IQADLGAAYTGQFVFNLLAPEPMPWAVEHYNALCLRFIAPIEQQLGATRYIAGDEYTI
                     ADVLGYPLATVSMLRYPGNLDAYPNMRRWVAEVGARPAVQRGMKVGT"
     gene            complement(16354..17292)
                     /locus_tag="PKN91_04945"
     CDS             complement(16354..17292)
                     /locus_tag="PKN91_04945"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HNS27455.1"
                     /translation="MKNLLLPTALSLQAAMAQTPEPSQPDRVAEPVEEIIIAAPEPRY
                     VAPTLRDRIGRVWAPVYVNGQGPLRLVLDTGASRSAVTTDAAQRLGLPVETAPTVLLR
                     GMTGEAQAPIVDVERLEIGDLTIEPAKVLVVPDAFGGAEGVLAARGLKDRRIEIEFRN
                     DRISIARSRNQRAPAGYTSLPLRLIRGHVPTISAQVGRIPVRAIIDTGAQQTTGNLAL
                     REALQARRRLEEESDVIIGVTGDTQEGATSSVPPIYLGNVTVKNAQITFVDLFIFQHW
                     RLTEDPAIMIGMDVLGILDTMILDYRRRELQIRLVQ"
     gene            17361..18719
                     /locus_tag="PKN91_04950"
     CDS             17361..18719
                     /locus_tag="PKN91_04950"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HNS27456.1"
                     /translation="MPWLTKSRFMSGRQCAKRLWFEVNAPLEVREPESLAILQGRAFD
                     DFVRKLWPGVVIDRERGMPAAIAQTSRVLAAGGVPVLHQAAVRNGEFAIIADVLRSDR
                     GGAELVEIKASTSVRPEHVADAAFQVLVLRGARVRVERARIGHVNSAFTLQRAGEYHG
                     LLIEQDITAEVEALLPALADEAIVLHRVMSARETPQIAMGPQCRVPYECPFIGRCSGG
                     APASDLSTHKDPRVRAGAADGTTSFDPGQAAVLRGHPWPRSYLDFETIGFAVPEVVGT
                     RPYEPLPFQWSLHIEAADGGLRHAEYLATRQFGDFAALSKALIAAVPDEGPVYAYNAS
                     FEGRALELLGRLAPEHKAELARLRARLVDLLPITRRAYYHPRMMGSWSIKAVIPTITA
                     ALAYDTLGGVQDGEAAQRAFLELRNGGPDEPRRAQLEQELLAYCRRDTEVLVTLARFL
                     QS"
     gene            complement(18710..19102)
                     /locus_tag="PKN91_04955"
     CDS             complement(18710..19102)
                     /locus_tag="PKN91_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27457.1"
                     /translation="MAKPGTSKSAHRERREKSLRARAASDTMRDAYPSVDHLRLTLTF
                     VDVTPTPAPQSFVLHPPARAYFEFPCPYSDCDGRFDLAAIARDTIKPGRKHAEGTLVC
                     SGERTRGRIPKQPCELRVDYSVDVVYQD"
     gene            complement(19211..20032)
                     /locus_tag="PKN91_04960"
     CDS             complement(19211..20032)
                     /locus_tag="PKN91_04960"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HNS27458.1"
                     /translation="MPVPDVRVESLAFYPIKGCRRISCQTAELLPSGLPHDREWMLVD
                     EKNTFITQRTDPVLATVSPTLSHGALTIDAPGRERLTLPLDRRGRRLAVRVWRDEVEA
                     DDMGDEAADWFTAVAGRPVRLVRYALDAARVADPAYALDAYAPVRFPDGFPILVTLTA
                     SLAALNAELDTPLPMTRFRPSIVLDGLGAFDEDRIDTLRIGDAELRCVKPCARCIVTT
                     TDQLTGERAIEPLPTLKRLRWNRELKGVTFGENAVVLRPGRLSVGDAVRVSWRTR"
     gene            20072..20710
                     /locus_tag="PKN91_04965"
     CDS             20072..20710
                     /locus_tag="PKN91_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNS27459.1"
                     /translation="MKLIGMFDSPFVRRVAITMHVHGMPFEHLDWSVGRDFARIREYS
                     PLGRVPALVLDDGEVLVESAAILDYLDEQAGPQRALLPASGVERRQALQLMALAVGAA
                     EKGRDQIYESAFRPPEKRHEPWLERCRAQMQSALAELDRASALRGASAWLVGSRMTQA
                     DITTACMVTFLTESVGVAPEAAPYPALRTLVARCEALPAFSAFHAPWFSPSP"
     gene            complement(20700..>21287)
                     /locus_tag="PKN91_04970"
     CDS             complement(20700..>21287)
                     /locus_tag="PKN91_04970"
                     /inference="COORDINATES: protein motif:HMM:NF016240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HNS27460.1"
                     /translation="SLNALTNACNQKSNRDPVLALDEAEVQRTLDTLSRKHLVIERAG
                     FGSRVPKYQQCFCNTEYGSLKFTPVELGIVCELLLRGPQTPGELRTRVPRMAPLGDPA
                     EIETALQALAGRESGPLVVRLAREAGRRDARYAHLFSGDIEAGEDAPLPAAPPPAPSA
                     AQAVASADDARLARLEAQVSALRAEVDELKSRTTG"
BASE COUNT         3271 a         7134 c         7571 g         3311 t
ORIGIN      
        1 gcttctcgag gtggtaggcg atgtcggaag cgaaataggt cttctcgccg ttcgcgcgca
       61 ccacgacgcg atcctcgtcg tcaccgaatt cggtggcgcg aaaccaggtc gcgccgtcct
      121 tctcgtagag ccgaccctgg ccgcgcagca cgtcgagcgc gtggtcgatc gcgccgctcg
      181 tcgcgagcgt tcgctcggag taccagcgat cgaacacgac gccgaagtcg gccaggtcgt
      241 tcctgatgtc ggcgagcatc gccgcgagcg agaaatcgag cacgcgctgg taatcagaag
      301 cgccgagcag ggcgcgcata cgctcgatga tcgcatcgac atacttgtcc ttgtcgccgc
      361 ccgcgggttc atccggcggc aggccggaga acacgtcgct ggcgggacgg cgaaacgcct
      421 cctgctgcgc ggcgtgcagt gccgcgccca gcacgcgcac gtagtcgccg cgataactat
      481 tcgacgggaa ctcgagcgcc tcgccgcagg tttcgagata gcgcagccac acgctcgcgg
      541 tgaggatgtc catctgccga ccggcgtcgt tgatgtagta ctcgcgatgc accacgtggc
      601 cggtggcctc gagcaggttg cccagcgtcg caccgaaggc ggcctggcgc ccggtgccga
      661 cgtgcaccgg accggtcgga ttcgccgaca cgaactcgag gatgaccttg cggcccgcgc
      721 cgagtgtggt gctgagcccg tacttcgcgc ccagctcgag cgcgcgccac agctcgtcga
      781 gattggcatt cgcgtcgagg tggaaattga tgaagcccgc gccggcgatc tcggtgcgcg
      841 ccaccagccg gttcgcgggc agcgcggcga tgatctctgc tgcgagttca cgcggcgggc
      901 ggccggcgac cttggcgagg cgcagtgcca cgttcgaggc gaagtcgcca tgccgggcgt
      961 cgcgggtacg gtccaccacg atcgcggcgg gatcggcggg cggcaggctg cctgcctcgg
     1021 tgaggcgcgc cagcgccgcg gcgaggagtg tttcgagctc ggttttcaag gagttgcgtc
     1081 gtgagttggg aggcgcgcaa ttctagcgga acccggcgtc aaccggggta gtctcagctg
     1141 cgttattcgg cttgtacgca accctcgatg gagtttccta tgaaatcgac agtcatcgtt
     1201 ctcgccctga tggccgcgcc gctcgcggtg ctggctgacg acacggagcc cggccgcggc
     1261 cacatgggcg gctaccagat gggcggcaag aacctcatcc agacgctcga cgccaacaag
     1321 gacggcaagg tctcgcgcga cgagtcgagc aaggcggctg tcgagcgcgc caaccgccgt
     1381 ttcgaccagc tcgacgccga caaggacggc ttcattacgc aggccgagat cgacgcggcg
     1441 cgcgacaaga tgcgcagcga ggtgcgcggc gagatgagca ggcgcggcgc cgagaactgg
     1501 aaggctgccg acaaggacgg cgacggcgcg atttcgcgca gcgaagccga agccggcatg
     1561 cccatgctgt tccgccgctt cgaccagctc gacgcgaaca aggacgggaa gatcagccgc
     1621 gacgagatgc cgcagggcaa gcgcatggac gacggcagga aggggcctcc gacgagctga
     1681 tccagagcgc tctgggctga tccaccgggc gcccggccaa cacgccgggc gcttggcatg
     1741 gccgcgtcct gcgttcagac tatccgcaat cagcgctccg ccagatcgcc tgcaggcgac
     1801 cgagagcgcg ctcgaaatgt ctgcatacag ggagtggcag gtaatgagta atcgtctctc
     1861 gtggctgtcc ggcgcgctcg tcgccgcgac gctcattctc acggccgggg cgagtgccgc
     1921 ggagcccgtt ccggcggcag gccgcaaggt cgtgctggat ctcgcgggtg acaggttcca
     1981 atggaagatc gtggaggcgc cggtggcggc tccgggtccg caccaggtgc tcatgcgggt
     2041 gcgcgccgtg gcgctgaacc gcggcgatgt cgaggtcctc gatccgaagt tcggtgcgaa
     2101 ggcgggcctc gtggtcgcct ccgacgcagc cggggagatc gttgccgtcg gcagcgaggt
     2161 cagggatttc cgtgtgggcg agcgcgtgaa cacgctctat ttccggaact ggaccgacgg
     2221 cccgccgagc ggggagaagc tggccgcgac gcacggagcg acggtcgacg gcgtgctggc
     2281 cgattacctc gtgctggatg acaccgccat cacggcaatg ccgaatggcc tgagcttcga
     2341 ggaagccgcc accctgccga ccgccggcct cacggcgtgg atggcggtca acgggcaccg
     2401 ggcgctgcgt gcgggcgacg tcgtggtggt gcagggcacg ggcggcgtct cgacgctcgc
     2461 gctgcagctc gcgaccgccc ttggcgggcg cgtcatcgcg accagcagct cggacggcaa
     2521 actcgcgcgc gccagggcgc tcggcgcgga gggcggcatc aactatcgaa ccacacctgc
     2581 atggtcggca cgcgtgctcg agctcaccgg cggccatggc gccgatgtcg tcgtggatgt
     2641 cgtcggcaag gcggcgctgc cggagtcgct gcagagcctg gcgtacgggg gcacgctgtc
     2701 cctcgtcggc gggctcaccg gcttcgacgg tcaggttccg gcgatccagc tgctgctcaa
     2761 gaccgctcgc gtgcaaggca tctgcgtcgg ctcgcgcgcc gactaccggc ggatggcgga
     2821 attcatcggg caacgcgaat tccacccggc gatcgaccgt gtgtttccgc tcgaggagta
     2881 cgcggcggcg ctggcgcaca tggaatcggg ggacttcgtc ggcaaaatcg tgttgacgct
     2941 ctgaaggcgc tgccggttca gaacagttcg agcgggtcca cgtcgagtgc ccagcggact
     3001 cgacgtgcgc tcggcagcgc ctccgcgagc ggcagccagg catcgagata gcgatgcagc
     3061 gccgcgcggt cgctgctctc gacgagcaac tgcgcatgga agcgatcggc gcgcctcgcc
     3121 atggccgccg gcgcgggccc gagcagcttc acctgacgcg gtggcgcgag taaacgccgc
     3181 gcctcgccga ggaattccac cgccgcatgc agggtgagcg ccgaggcgcg caccgcggcg
     3241 agccgcccga acggtggcca gcacgcgacc tgccgctcgg cgagcgccgt cgcggcgaaa
     3301 ccttcgtagc cttcgctgag caggctcgcg agcagcggat gctcgggata ttccgtctgg
     3361 atcagcacct cgccggcgcg cgcaccgcgt cccgcgcggc cggccacctg cacgatcgtc
     3421 tgcgcgagcc gttcggcggc gcgaaagtcc gtgctgaaca ggccatggtc ggcgttgagt
     3481 accaccgcaa gggtcacgtc ggggaaatcg tggcccttcg tcaccatctg cgtgccgacg
     3541 aggatgcgcg cctcgcccga ggcgacgcgc gcgagcactg cggcgagctg ctcgtcgcgc
     3601 cgcacgacat cgcgatcgag ccgggcgagg gccacgtgcg ggaacagctc cgcgagcgtg
     3661 tcctcgaccc gttccgtgcc ctggcccacc gcgcgcactt cgtagccgca ttgcgggcag
     3721 cgcgtgggca gtgccgcgtc ggcaccgcag tgatggcatt tcaggcgcga ggcgcgcgcg
     3781 tacaccgtca ggcgcgcatc gcaggagctg caacctgcga tccagccgca ggccgtgcac
     3841 gcaagggtcg gcgcgtagcc gcgccgattc aggaatacga gcacctggcc gccatcgtcc
     3901 agatgacggc ggatcgcctc caccacggtg gtggacaggc cgtggcgcag ggcctcggcg
     3961 cgcaggtcga cgagcgtgag tcgcggcggc agggcctcgc cggtgcggcg tgacagcgcg
     4021 aggcgcgtgt agcggccggc ttgcgcattg tgcagcgtct cgagcgaggg cgtggccgag
     4081 cccagcacga tcggcacgcc ggcgcgctgt gcgcgcatca ccgcgatgtc gcgcgccgag
     4141 tagcgaaagc cgccttcctg ctgcttgaag gatggatcgt gctcctcgtc gacgacgatg
     4201 agcccgagtc ccgggagcgg cgcgaatact gccgagcgcg tgccgagcac gatgcgcgcg
     4261 cgcccgctga gcgcgtcgcg ccaggcgttc aggcgcgccg tgccggtgag ccccgaatgc
     4321 atcgtcgcca tcgccacggc gaagcgcgca gcgaagcgcc cgatcagttg cggcgtgagc
     4381 ccgatctcgg gcaccaggat cagcgcccgt tcgccgcgcg cgagcgcgcg ctcggtgagc
     4441 cgcagataca cctcggtctt gccgctgccg gtgattccct gcagcaggaa agcgccgaag
     4501 cggccgtggc cagcttcgat cgcatcgacc gcggcctgct gttcgcccgt cagctcgaac
     4561 ggcgagccgt tcgcgatggc gccggcgccc gtctcggcgg gagcggcctc gtcggcgagg
     4621 tcgcgcgcgc aggcccagcc gcggcgggtg agggcggtga gtgcctcgcg cgcgcccgtg
     4681 ccgagcgcgg cgagggcggt cgcatcgagg ctgccgcgtt cgatcagttc ttcgaggatc
     4741 gcgcgctgcc ggggggcgcg gcgcggctcg ccggcggtat gcgcggcgat gccctctgtg
     4801 gtcggtgacc agacctcgcg caccgcgcgc gtgcgtgcgc cttcgcgcgc gagtttcggc
     4861 agcgccgaaa acagcacctc gccgatcggg tggtggtagt aatcggcggc ccagcgcagg
     4921 agcgcgagca gcccttcgtc gaacacgggc tcggcatcga gcaccgcggc gacaggtttc
     4981 agtttcccgg gcggcagcgc cgagtcgaca ccgatctcga gggcgatccc gataatttgc
     5041 cggcgcccga acggcacccg cacacgcgca ccgggccgcg gcacctcctc cccggcgaga
     5101 tagtcgaatg tcctgcgcag cggcgtatcc accgccaccc gaatgatcga aagcccgacc
     5161 aagaaaaccc tttgcaaaac aacaagttga ttaaagctgc ggcgccgggt tccgaatctt
     5221 tcggtcatgg gcagccgtca acggcgccgc gcctgccacg ttactttctg tatgaacgca
     5281 cttgcggcgt cgccgcgcgc ttatagcatg agctttgccc acggcgacgg ccacgacatg
     5341 cctgatgctc ttcgcgcgcg gaatgccgcg ttcgaccgct tcttcatcgc cgtcgagcgc
     5401 aaggcgtttc gcatcgcgcg gcttgccctg cagaacgacg atgacgcgct cgacgccgtg
     5461 caggactcga tgctgcagct ggtgcgcgcc tatgcctcgc gcccgcaagc cgagtggaag
     5521 ccgctgttct accggattct tgccaaccgg acccgcgatc tgcagcgccg gcgcagcaca
     5581 cgcgggcgcc tatttgcctg gtggcccacg ggaggcgaaa ccgacgatgg cgacgatccc
     5641 gccagcgccg cgccgagccc ggagccgggt ccggaacgga gtctcgagct cggccagact
     5701 atggccctat tggatcaatt tataggacaa ttgtcacata aacagcagca agcgtttctg
     5761 ttgagaaact ttgaagacct cgatgtggcc caggccgcca gggccatggg ctgttccgag
     5821 ggcagcgtga agacccatta ttcgcgcgcc gtacaccgcc tgcgcgcacg gcttatggag
     5881 gccgaatcat gaccaccgga cgtgaaatcg agcggcaggc gcgcgagacg ctcgaggcga
     5941 gcatcgagca tctcgacgcg cgcgtgcgct cgcgactcac ccgggcgcgc catgcggcgc
     6001 tcgatgagtt gcagcggcgt gcgcgccgcc cggcatggtt cgtcgtgccg gccgtgggct
     6061 tcgctgcttc ggccatcctt gcggtcgtgc tgtgggcgcc gtggtccact gtccaggatc
     6121 cggccaacgg caccgcgttg actgcgctcg cgaccccgga cgctgatctt gatttcctgc
     6181 tgggcgagga cgtgttcgcc gcggtcttcg aagacccggc gggctaggcg ccgcggcgca
     6241 tgccccgagt gggtgtccgg tcactcgcgc gccagctcgc tccccgccct caccacatcg
     6301 gccagcgtgc ggcagccgag cttctccatc atgcgcgcct tgtagacctc gaccgtgcgc
     6361 gggctgatct cgagccgctc ggcgatctcc cgcagctggt tgccctgagc cagcaggcgc
     6421 agcacgtcgc gttcgcgcga ggtgaggcgc gccaggcgct cggccgccgc ggcccgcgcc
     6481 tgcgccgcgg cacttcgctc ggcgtcgagc tcgatcgcgt tgcggagcac gtcgatcagc
     6541 acggcgtcgt cgacgggctt ctcgaggaag tccaccgcgc cgttcttcag cgccgcgcgc
     6601 gcggtcgcga cgtcgccgtg tgcggtcagc acgatgaccg gcagcgggat gccgcgcgcc
     6661 ttgagctcgg cctgcagatc gagtcccgac atgccgggca tctgcaggtc gacgagaacg
     6721 catccccgcc attcgctgcg acaggtgtcg agaaaactct ccgccgaggc gaatatctgc
     6781 gcacgcaggc cgtgcaggga aatcagcagg ctcagcgcgt cgcggatcgc agcgtcgtca
     6841 tcgacgacga agacgacggc ttcactgttc atcggcttgc gccaccttca gtcgcagggt
     6901 gaaacgcgcg ccgcccgtcg ccgcgaccgg ttcgtactcg agatcgccgc cctgcgcgcg
     6961 caacagcgag cggctgatgg cgagcccgag tcccatgccg cccggcttgt cggtgacgaa
     7021 gggctcgaag atgcgcggcg cgatctcggt ggcgacaccg ggcccggagt cgaccacgct
     7081 cagcagtacg tcgtcgccgc gttgtgccgc ggtgatgtcg atgcggcctg atgcgtcggc
     7141 ggacagggtg atcgcatcga tcgcgttcga gacgaggttg tgcagcacga tctcgagctg
     7201 ggtcggatcc gccgcgactc tcggcaggcc ggcgtcgaga ctgcagacga gttgcacgcg
     7261 cgcgcgccgc agacgctcgt gcagggccgc accgacgatt tcgcacatcc tggcgagcga
     7321 cagcggcgcg cgcgcggccg cgccggtgct gtagaagtcg cgcaggcggc gcagcacctc
     7381 cgaggcgcgc agggcctcgc gcccggcctt gtcgagcgtt tcgggcaggc gcgcatccgg
     7441 ctgcgcggcg agcatttccg cggcccgcag ataagagacc agcgcggtga tcggctggtt
     7501 cagttcatgg gtcagcgccg aggccatctc gccggcggtg gcgaagcgca tcgcgcgggc
     7561 gagcgccgcg tcgcgctcgc gcagctgctc ctgcgcgcgc tggcgctcgc tgaccgcggc
     7621 gccgagcagc agcgcggtga ggttgagtgt cagcatcagg aactgcaggt cgagcagcgg
     7681 cgcggccatg cctgcctcgg tgaccatcag cgcaagtccg atctggatca cgagcgcgcc
     7741 gaccactgca ccgacgatgc cccagcgcag cgcgatccag atcatcggca cgaacagcgg
     7801 atagaagaag cgcagcgcct ggtcggacag caggccgaag atgatccaga tcgtggcgcc
     7861 gagtacggca acctgcagca gcgcctcgcg ccagtgcgcg cgcagcgccg cgagtccggc
     7921 gcgccactgc ggcgcccata tcagcagcgg tgtcagcgtg aggacgccgt tgaagtcccc
     7981 cacccagtag cgcgcgacgc cgccgagcgc gacctccccg ggcagcgcgc ccatcgccag
     8041 catgatcgcg atgaagcccg cgccgacgag cagcgtggcg acgaggctgg cgccgacgaa
     8101 gcgtgcggcg tcgccgggcg agggcaggaa gcttgtgcgc ttgcgccgca gtacttcggc
     8161 cagcgccgcg tatgcgatcg cgatccagat cgaggtggtg atggccgcga tgctgcccgc
     8221 gggcacgtcg cgcacgacga tctccgacag caacgccgcg gcggcgacgg cgaaggcgcc
     8281 gcggcgtcct tcccagaaga ggaatgcgag cgcgacaccg gtctgcggat tccacggcgt
     8341 gatgccgagc tgcagcaccg gctgcagata gctgagccag tcgatgagga cgtaggcggt
     8401 gatgaagagt gcggcgcgaa ggagatggcc ggagccgggc atggggtacg cccggctccg
     8461 gccggaatcg ggtccgcccg ccaggacggg cgcgccggat tccccactct ctcggggaga
     8521 aaggctaggt gccggctccg gcgtgggcaa ttcggcggcc cccgtacggg cttgcccgta
     8581 tgcactggcc atggcttacg gagccaccag cgctccggcg cgctgcagcg cttcctgcgc
     8641 ttcgacgttg cggccgcgcg cgcgcagcgc gaagccgatc agctgccagg cgctcgcctg
     8701 ttcgcgcggc gcaccggcgg cgagcgccaa tgcgcgccgt cccatgttct cggcctggac
     8761 ggggtcgccg ctcgcgagtc gcatctggcc catttcgatc cagacgagcg gattgtcggg
     8821 ctcgatgcgc agcgcgcgct cgagcgtggc tgccgcggcg tcgaagtcgc ccttggatgt
     8881 cgcggtgcgc gcctgcacga cgagcgcgtt ggtcgccggg ctcagtcgat ggctcctggg
     8941 tggcggcgcg ggtggcggaa ccggagccgg cgtcgccggc ggcgtcggct cgggcacggc
     9001 gctgccgggc tgctcgggcg tcgtcgtgcc gggctggctc ggtgtcgtcc cgctggacgg
     9061 ccggtaaagg ggggtcgaac aggccgcgag cgcgagggcc gctagcaaca gtggcgcggg
     9121 gaaaaggaaa cgtcgcatca gcggatgatg ccacggagcc agtcgccgat cccggatggc
     9181 ggggcgcagc cggccaggaa gggcggggag gtcccgcgcg gcaccgcgac gatcacttcg
     9241 tcctgcgagc agccggtcgt gacacgcagt ccggaggcat aatcgatcgg caggtcctcg
     9301 agcgattccg gcatcggcgg ctgccacgag ctcgtgtcga gtgcggacat gatcttctgc
     9361 cacacgatca gcgatccgct cgagccggtg acgccggtcg ggcgattgtc gtcgtagccg
     9421 atccacacga ccgcgaggtg tgtgcccgag aagccggcga accagccgtc gcgcaggtcc
     9481 gacgagctgc cggtcttgcc cgcgacgatg agctcgcgtg gcagccgcgc ggcggcggcg
     9541 cgtccggtgc cgcgcgcgat cgcgtaggtg agcatgcgat cgatctgata caccgcgtcg
     9601 ggcgcggcga ccggctcgac cttcaccggg taggccccga tgcgctcgcc ctgctcgttg
     9661 accaccgcgc gcaccgcgcg cagcggcacg tggaagccgc cgttcgcgag cccgttgtac
     9721 atctgcgcga cgtcgatcgg tgggaggtcg accgcgccga gcagcagcga gggcagggcg
     9781 cgcggctcgg tctcgaggcc gaagcgcgtg aacatctgcg cgaccttcgg cacgccgaca
     9841 tcgagtccga ggtgcacggt ggcgaggttc agcgattcgc tgagcgcacg caccatcgac
     9901 accggcccgt tggcgttgcg cgagaaattc tgcgggcgcc atacattgtt gcgcgacagc
     9961 ggcacttcga tcggcgtgtc gtcgagtacg ctggtcgcat tccagcggcc ggtctcgatc
    10021 gcggcgaggt agacgaacgg cttcaccagc gaaccgatcg gccgcgcgct cgcgagtgcg
    10081 cgattgaggc cgtcgaagct cacgttgcgt ccgcccacca tggcgatcac ctcgccgctc
    10141 tgcggcagcg tgacgatgct cgcgccctcc agcccgggcc ggccctcgtc ggacctggtc
    10201 cgcttgtcga gccgtccgat ctcggattcg agcgcctgct cggccgcgac ctgtgcgcgc
    10261 ggatcgaagc tcgtgaagat gctgagcccg gcctcggtga ggtcttcctc tttgtagtca
    10321 cgtcgcagcg tgcggcgcac gaagtcgaga taggccgggt agtagccgcc cgaggggcgc
    10381 gcggcgacgc cgagcggctg cgcggtggcc ttgcgcgctt cctcggcact gatgagctgc
    10441 tgctgcgcga ggatgcgcag cacgaggtcg cggcgctgct gggcgcgccc gggatggcgg
    10501 cgcggatcgt agtacggcgg gccgcgcacg atcgcgacga gcagcgcgag ctcggccgtg
    10561 tcgagctcat cgagcggctt ggcgaaccag aactggctcg cgagtccgaa gccgtggatg
    10621 gcgcgatcgc cctgctggcc gaggtagatc tcgttgatgt aggccgtcat caggtcgcgc
    10681 ttgtcgtaac gggcctcgag cgcgagcgcc atgaaggcct cctcgacctt gcgcccgagc
    10741 gtgcgccggc cgtcgaggaa gtagctcttg accagctgct gcgtgagcgt gctgccgccc
    10801 tgctcgacac gaccggcgcg cagattcacc cagaaggcgc gggcgatcgc ctcgaagtcg
    10861 aggccgtggt gagcgtcgaa cttgcggtct tcgaccgcct tgatggcggc cggcagcatc
    10921 tgcggcactt cctccggcgt gagcacgatg cggtcctcgc cgtggatcgg gaagatgctg
    10981 ccgatcagca gcggatcgag gcggaagatc gggatgtcat tgccgcgcgc atcgcgcagg
    11041 ctggcgatca cgccacgttc gagagtcagc gcgatcacct gcgcggggcg cagctcgtcg
    11101 gagaagcgcg cttcgcgcac cgcgatctcg aagcgcccgc ggcggcggcg ccaggtgccg
    11161 ggccgggtga gcgtgttcgt ctcgcgatac tgcagccggt cgagctcggc aatgagctcg
    11221 tcggtcgaga aggcgaggcc ggcgtagagt tcgagcggcg ccgcgtacac cttggcgggc
    11281 agcgtccagc gccggccttc gaactgcgcc gcgacctgcc ggtcgtagta gaagagtgcg
    11341 atcacgaacg cgaccgccac cgctccggcg agcgcgccga cggcgatccc ggcattcttc
    11401 aggatgttct tcgtgcggcg cttcatggcg ccatgttaat gaaagccggg ccgcacatag
    11461 gcgccgaatg tcaggtagaa ggattgctcg ccgccttccg cgtagccgaa gccgaaccgc
    11521 agcggtccga gcggcagcgg cacgccgacg aatacgctgc ccgcccggat caggtcacgg
    11581 gcggcgatgt cctcgaagtc ctcccacaca ttgccggcct cgaagctggc accgacatag
    11641 agcggcatcc tgaagagcga tcgggtactg ccgaagctgc ggtagtagat gagccggccg
    11701 aacaccatct ggtcgccgag caactggtcc tcgccgtagc ccgagagata ggtgaggccg
    11761 ccgaggttcg ccatggtgct gaacgcggcg atgctgtcgg tggacaccga ggcacgcgcg
    11821 ccgatcgaga cggtattgcg gccgaaggtg aacgcctggt ccagggcgag gcgcgcgaga
    11881 tcgccgcgct cgtgggcacc gagggccgcc ctgagccagc cgtagctcgc ctcgaggcgc
    11941 aggccgcgcg tcgggaaggc gacattgtcg agcgagtcgt acaggaattg cgcgcgaatg
    12001 cccgcgacat cggcgcgcat cgattccggc aatgagggat cgccgacgag cagcttgccg
    12061 cgatcgtgga agcgcgacgc gaccagcgac aggcgcaggt tctcgccgaa gtcgcggccg
    12121 aaacgcagct cgccgctgcc ggtgtccacc cggtagcgcg cgatctcggt atcgccgagg
    12181 cgcgtcggct ggttcatcgc gatgtactgc agttgtggtg cgatgaacca gcggccaagc
    12241 ttgccgaagg gcgcgtagta ctcggaggtg atgccgctct cgcggccgag ctgcagcagg
    12301 ttccgccact cggccccggt cgagctgagg ccggtcatgc gcacttccgc attgaactgg
    12361 taatcatcgc ggccggcaaa gtcgtcctcg aactgcagac ccatgcgcag ataggtcggg
    12421 ccccaggctt tgtcgatcgg ccgcacgatc aggccgaact cgccgtcgcg ctcctcgagt
    12481 cggtagtcga tgcgctcgta ggtgccgccg ccgaacgcgc ggccgatctg gtcggtgacc
    12541 gcctcgaggt cgaagcgctt gtcctcctgg actccgagcc ggtcgcgaac gagcccggcg
    12601 gtccggctcg tgtcgctgcg cacgtcgacg aacgcgatgt gcggattgcc gagcggcttg
    12661 cgcgcctggg tggcgcggtg cgccgcgaac tcctcggggc tcgcggcgag cgcgcgcagt
    12721 tccccgatgt gggccgcgac cgcggcctcg cctgcgagca cggcctcgcc gaccttggcg
    12781 aaatcgagcg aggcgatatc cggcatgtcg gggcgcagtt cgacgtcgcg ctcggtcagc
    12841 gtggcgagca cgcgcgcgct ctcgcgatcg acgaggcccg agagcacctg catcataatc
    12901 gacaccggcg agccgagctt gtcctcgggc agcagcggct cgctcacgtt gacgacgatc
    12961 agccggtcgg cgcccatggc gcgcgcgacg tcgacgggga tgttgttcgt gatcatgccg
    13021 tcgacgagca ggcggccgtc gtagcgcacc ggcgcaaagg cgccggggat cgatgcgctc
    13081 gccagcaccg cggccgtgag gtcgccttcg ctgaacacca cctcctgcgc ctcgccgacg
    13141 tcggtggcga cgcagcggaa cggaatcggc aggctgtcga agctgatgcg ctcctcggtc
    13201 tgcaccagca gttcgcgcag gatgatgttg aatttctggc cgaccacgat gctcggcggg
    13261 agtttcacgc cgtcgcggcc gatgccgatt tcctgcggga tcagctgcgc cccgtcgagt
    13321 tccttctcgc gcatcggcat cgcatcgcgc gtctcgccgt cgcggaacac gtcgcgccag
    13381 tccatggtgt cgagaatgtg ctggatctcg tcgaccgtgt agccggaggc gtacagcgag
    13441 ccgatgatcg cgcccatgct ggtgcccgcg atgtagtcga tcgggattcg ttcgcgctcg
    13501 atggcgcgca gcacgccgac gtgcgccgcg ccgcgcgcgc cgccaccgcc gagcaccagg
    13561 ccgatccgat cacgcgccgt cgcgatcggc gtgatgagca cactcaggag cgccagggcc
    13621 gcaacaggcc ggcgccaccg cgatttctgc cgcatggggt cacctctcgc gcaagcgtag
    13681 cgtgaatccc ggccgccgcg ataatggtcc ggcgagagct gatgcgcgcg ggatcgtgcg
    13741 gggcaagggc acggggcggc gggcgccggg ccggtgcggc ataatgtcga gcaatggagc
    13801 gcgattacca ggccccatgg ggcacgatgg tgcgcatcgg cacctgggcg acgctggcgc
    13861 tgctgggcgc catcgccgcg atgggcttgc tcattggccc gcggcaacaa ttggcatgga
    13921 aactcgcgat gatcggtgcc ccgttgttgc tcgtcgcatc gacactgccg ttcgcgatac
    13981 gcggctacac cctgacgggc cgcaatctcg tcattcgccg cctcgggttc gacaccacgc
    14041 tcgcgctcga cgacctgcgg gtcgtgcgcg gggtggccga cgatgtgcgc ggctcgctgc
    14101 gtctctgggg caacggcggg ttgtttgcga tcaccggctg gttctggaac cggcggctcg
    14161 ggcgcttccg catgtttgcg accgacctgt cacgcgcggt gttgctggag tttgcacgcc
    14221 gcaaggtgct cgtcacgccg cacgatccgc aggcgttcat cgtgcacgcg cgcaaactga
    14281 tggcgatgcg ttgagcggag gggacgatgg ccgatgatcg aatcgagctg ctcgaaacga
    14341 aactggcgca cctcgagctg gcgctgcagc agctgagtga cgagatggcg cgccagcaga
    14401 aggacatcga gctgctgcgc gagcgcggcc gccagctcga gcggcagatc gaagccgcgg
    14461 cgcaggaggc ggccggtgcc tcggccaccg cgatcgaaat cccgccgcac tactgagcgc
    14521 ggctcgccac cagtttctcg tcgatggccc gcgcccgcgc caccgagggc cgtgcattcc
    14581 agcgctcgac atagcccttg atcggctcat cggccggcac caggccgaac gcggtgatcc
    14641 aggtgagcgc cgtgccccac agcacatcgg ctgcggtaaa acgctcgccg agcaaccacg
    14701 gcccctgtgc gagctggccc ctgagcgtcg cgatcgtggt gtcgtaatcg ccgtagggca
    14761 cggtcgccgg cgggccgggc tcgcgcttca gcgcgcgatc gacgaccgcc ggctcgaagg
    14821 ccgaaccgta gaacaccagc cagcgcaggt acggtccgcg cagcggatcg ccgatcggcg
    14881 gcgcaagccc ggcggcagga tagaggtcgg cgaggtagat gaagatcgcg ccctgttcgg
    14941 tgacgagggc atcgccgtgg cgcacggccg gcaccttgcc catcggattc accgcgaggt
    15001 attccgggcg gcgctgctcg ccgcccttca tgtcgatcac ctgcaggtcg tagggcacgc
    15061 cgagttcctc gaggagcgcg agcgtgccgg tcgagcgggt gttcggagaa tggaaaaaaa
    15121 cgagccggtc ctgcgatgtc atctgcgcct cccagcgccg ggttctacca ggtaatgccg
    15181 ccgatgtagc cgcctgccgc cgggaagaac ggcagcactt cgatcgtttc ccacacgccc
    15241 gccctgaaat acgggtcgcg ctcggtgagc tcgcgcgcct gccgttcgct ctgcgtgcgc
    15301 agcagcagca cgctgccgat cggacgcttg cccgcctcgt cgaacagcgg ccccgcgaca
    15361 tgcagctcgt cgatgacctg ctcgatgtgg cgcaggtggt cggcggtagc tcccttgcgc
    15421 ttctcggcgc cgtcgggcgc atcgcggcag atcacgatgt agcaggcggt cggatatctc
    15481 tcgtgtacgg gtgtcgtcat ggcgggtctc atccggtggg cgggatgttc tcatacatcg
    15541 gaaaaccctg atcgagtgat gccggaagac tgggacatac tggctctctc ggcaacccga
    15601 gttgctccga ttcccacaaa ggcgacttgt tcaatgatag acctgcacac gacccccacg
    15661 gccaacggtc tcaaggcctc gatcatgctc gaggagaccg gttacgagta tcgcgtccac
    15721 aagatcgccc tcaccaagcg cgagcacctc tcgcccggct atcttgcgat caaccccgtc
    15781 gggcgcatcc cgacgatcgt cgatcatgac acggcgagcg gcaatccact caccgtgtat
    15841 ggcaccgcgg cgatcctgct ctatctcgcc gagaagtcgg gcaagctgct gcccgcggac
    15901 ctcgaggcac gggcacgcgt cttccagtgg ctcggcatca tccaggccga cctcggcgcg
    15961 gcctacaccg gccagttcgt cttcaacctg ctggcgccgg agcccatgcc ctgggccgtg
    16021 gaacactaca acgcgctgtg tctgcgcttc attgcaccga tcgagcagca gctcggggcg
    16081 acccgttaca tcgcgggcga cgagtacacg attgccgacg tgctcggtta tccgctcgcc
    16141 accgtgtcga tgctgcgcta ccccggcaac ctcgatgcgt atccgaacat gcggcgctgg
    16201 gtcgccgaag tgggtgcgcg cccggcggtg cagaggggca tgaaggtcgg gacctgaagg
    16261 ccccgccccg gtcacccggg ttgcagcgcg aggcggtcgg ccagccgttg tccggcatgc
    16321 atgcgtcccg gcatgttgcg gcggatcccg aacctattgc acgagtcgga tctgcagttc
    16381 ccggcgcctg tagtcgagga tcatcgtgtc gaggattccg agcacgtcca tgccgatcat
    16441 gattgcgggg tcctccgtca agcgccagtg ctggaagatg aacaggtcga cgaaggtgat
    16501 ctgcgcgttc ttcaccgtga cattgccgag gtagatcggc ggcacgctgc tcgtcgcccc
    16561 ctcctgcgtg tcaccggtga caccgatgat cacgtcgctc tcttcttcga gccgccgccg
    16621 cgcctgcagt gcttcgcgca gcgcgaggtt gccggtcgtc tgctgcgcac cggtgtcgat
    16681 gatcgcgcgc accgggatgc gtcccacctg cgcagagatg gtcgggacat ggccgcggat
    16741 caatcgcagc ggcagggacg tgtagcccgc gggggcacgc tggttcctgg atcgcgcgat
    16801 cgagatccga tcgttgcgaa actcgatctc gatgcgccgg tccttgaggc cgcgcgcggc
    16861 gagcacgcct tcggcgccgc cgaaggcatc ggggaccacc aggaccttgg cgggctcgat
    16921 tgtcagatcg ccgatctcga ggcgctcgac atcgacgatc ggcgcctgcg cctcgcccgt
    16981 catgccgcgc agcaggacgg tcggcgccgt ctcgaccggc aagccgagac gctgcgcggc
    17041 atccgtcgtg acggccgagc ggctcgcccc ggtgtcgagc acgagtcgca gcggcccctg
    17101 gccattgacg tagaccggtg cccacacgcg cccgatgcgg tcgcgcagcg tgggtgcgac
    17161 gtagcgtggc tcgggcgcag cgatgatgat ctcttcgacc ggttcggcga cgcggtcggg
    17221 ctgcgacggc tcgggggttt gtgccatcgc ggcctgcagg ctcaacgccg tcgggagcag
    17281 caggttcttc accttgccag cataacgcgc ccgcaagtca ggggtgtgac gccgccggcg
    17341 caatgcggca aagtgcgccg atgccctggc tgaccaaatc ccgcttcatg tccggccggc
    17401 aatgcgccaa gcggctgtgg ttcgaggtca atgcgccgct tgaggtccgc gagcccgaga
    17461 gcctcgcgat cctgcagggt cgggccttcg acgatttcgt gcgcaagctc tggcccggcg
    17521 tggtgatcga tcgtgagcgc ggcatgccgg ccgccattgc acagaccagt cgcgtactcg
    17581 ccgcgggcgg tgtgccggtg ctgcaccagg ccgccgttcg caatggcgag ttcgccatca
    17641 tcgccgacgt gctgcgcagt gaccgcggcg gcgcggaact cgtcgagatc aaggcatcga
    17701 cgagcgtgag gccggagcac gtggcggatg ccgccttcca ggtgctcgtg ctgcgcggcg
    17761 cgcgggtgcg ggtcgagcgt gcgcgcatcg gccacgtcaa cagcgcgttc acgctgcagc
    17821 gtgcaggcga gtatcacggc ctgctcatcg agcaagacat cacggccgaa gtcgaggcgc
    17881 tgctgccggc gctggcggat gaggccatcg tcctgcatcg tgtgatgtcg gcgcgcgaga
    17941 cgccgcagat cgcaatgggg ccgcagtgcc gggtgcctta cgagtgtccg ttcatcgggc
    18001 gctgctcggg cggcgcaccg gcgagtgacc tctcgacgca caaggatccg cgcgtgcgcg
    18061 cgggtgccgc ggacggcacg acatccttcg atccggggca ggccgcggta ctgcgcgggc
    18121 atccctggcc gcgcagctac ctcgatttcg agacgatcgg tttcgccgta cccgaggtcg
    18181 tgggcacgcg accgtacgag ccgttgccgt tccagtggtc gctgcacatc gaggccgccg
    18241 acggcgggtt gcggcatgcc gagtacctcg ccaccaggca gttcggcgat ttcgccgcgc
    18301 tgtcgaaggc gctcatcgca gcggtgccgg acgagggccc ggtgtacgcc tacaacgcgt
    18361 cgttcgaagg ccgggcgctc gaacttcttg ggcgccttgc accggaacac aaggcggagt
    18421 tggccaggct gcgcgcgcgt ctcgtggatc tgctgccgat cacccggcga gcctactatc
    18481 acccgcggat gatgggctca tggtcgatca aggcggtgat cccgacgatc accgccgcgc
    18541 tcgcttacga cacgctgggc ggggtgcagg atggcgaggc ggcgcagagg gcgttcctcg
    18601 agctgcgcaa cggcgggccc gatgagccgc ggcgcgcgca actcgagcag gaactgctcg
    18661 cctactgccg gcgcgacacc gaagtgctcg tcacgctcgc ccggtttctt cagtcctgat
    18721 agacgacgtc gacagaatag tcgacgcgca gttcgcacgg ctgcttgggg atgcggccgc
    18781 gcgtgcgctc gccgctgcag accagcgtgc cttcggcgtg cttcctgcca ggcttgatcg
    18841 tgtcgcgcgc gatggcggcg agatcgaacc tgccgtcgca atcggagtac gggcacggga
    18901 actcgaaata ggcgcgtgcc ggtgggtgca gcacgaagga ctgtggcgcg ggcgtggggg
    18961 ttacgtcgac gaaggtgagc gtgaggcgca ggtgatcgac ggacggatag gcgtcgcgca
    19021 tcgtgtcgct cgccgcacgg gcgcggaggc tcttctcgcg acgttcccgg tgcgcggatt
    19081 tggatgtgcc gggtttggcc aatgatctgt ccgaatcgat cccagtgggt cgccatgaat
    19141 accgcggcga aggaagtcgc gggcgtcttg acactttaca ggaccaggcc ttctcccgct
    19201 tgactttcgg tcagcgcgtg cgccagctca cccgcaccgc atcacccaca ctcaggcgcc
    19261 cggggcgcag aaccacggcg ttttcgccga acgtgacgcc cttcagctcg cggttccagc
    19321 gcaggcgttt cagtgtcggc agcggctcga ttgcgcgctc tccggtcaac tggtcggtgg
    19381 tcgtgacgat gcagcgggcg cagggcttca cgcagcgcag ttcggcgtcg ccgatgcgca
    19441 gcgtgtcgat gcgatcctcg tcgaaggcac cgaggccgtc gagaacgatg ctgggcctga
    19501 agcgtgtcat cggcagcggc gtgtcgagct cggcattgag cgcggcgagt gacgcggtga
    19561 gcgtaacgag tatcggaaag ccgtcaggaa agcgcaccgg cgcatacgca tcgagggcgt
    19621 aagccggatc ggcgacgcgc gcggcgtcga gtgcgtagcg cacgaggcgc accggtcgac
    19681 cggcgaccgc ggtgaaccag tccgcggcct catcgcccat gtcgtccgcc tcgacttcgt
    19741 cacgccatac gcgcaccgcg agtcgcctgc cgcggcggtc gagcggcagg gtgaggcgct
    19801 cgcgaccggg cgcgtcgatg gtcaatgcgc cgtgggacag cgtgggcgac accgtggcga
    19861 gcaccgggtc ggtgcgctgc gtgatgaagg tgttcttctc gtcgacgagc atccattcgc
    19921 ggtcgtgcgg caagccgctc ggcaggagtt ccgctgtctg gcaggagatg cgccggcagc
    19981 ccttgatcgg gtagaaggcg agggattcga cgcgcacgtc tggcactggc atggctcgca
    20041 tcctaatcga gtgggggctt agaatcccgc catgaagctc atcggcatgt tcgattcgcc
    20101 cttcgtgcgg cgcgtcgcga tcacgatgca cgtccatggc atgccgttcg agcacctcga
    20161 ctggtcggtc ggtcgcgact tcgcgcgcat ccgtgagtac agcccgttgg gtcgcgtgcc
    20221 tgcgctcgtg ctcgatgacg gcgaggtgct ggtggagtct gcggccattc tcgactacct
    20281 cgacgagcag gccgggccac agcgggcact gctgcccgca agcggtgtgg agcgccgcca
    20341 ggcgctgcaa ttgatggccc tcgcagtggg cgccgcggag aagggccggg accagatcta
    20401 cgaaagcgcg tttcgtccgc ccgagaagcg ccacgagccg tggctcgaac gttgccgcgc
    20461 gcagatgcag agcgcgctcg ccgagctcga ccgtgcaagc gccttgcgcg gcgcaagcgc
    20521 ctggctcgtc ggctcgcgca tgacgcaggc ggacatcacg acggcctgca tggtgacctt
    20581 tctgaccgaa tcggtgggcg tggcgcccga ggcggcgccc tatccggcgc tgcgcacgct
    20641 cgtcgcgcgc tgcgaggcgc tacccgcgtt cagcgcgttt cacgcgccgt ggttttcgcc
    20701 tagcccgtag tgcggctctt cagctcgtcg acttcggcgc gcagcgcgct cacctgcgcc
    20761 tcgaggcggg ccagcctggc gtcatcggcc gatgcgacag cttgtgccgc gctcggcgcg
    20821 ggtggcggtg cggccggcaa tggcgcgtcc tcgcccgctt cgatgtcgcc cgaaaacagg
    20881 tgggcgtaac gtgcatcacg ccggcccgcc tcgcgcgcga ggcgcacgac gagcgggcca
    20941 ctctcgcggc cggccagcgc ctgcagtgcg gtctcgatct ccgccggatc gccgagcggc
    21001 gccatgcgcg gcacgcgggt gcgcagttca cccggtgtct gcgggccgcg cagcagcagt
    21061 tcgcagacga tgccaagctc gaccggcgtg aacttgagcg agccgtactc ggtgttgcag
    21121 aagcactgct ggtacttcgg cacgcggctg ccgaacccgg cgcgctcgat caccagatgc
    21181 ttgcgcgaca gcgtatccag cgtccgctgc acttcggcct cgtcgagcgc gagaacggga
    21241 tcgcggttgc tcttctggtt gcaggcattc gtcaacgcgt tgagcga
//
LOCUS       DAOQVY010000034        20897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_2980_length_20897_cov_5.729393, whole genome shotgun sequence.
ACCESSION   DAOQVY010000034 DAOQVY010000000
VERSION     DAOQVY010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 20897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20897
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2980_length_20897_cov_5.729393"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PKN91_04975"
     CDS             <1..428
                     /locus_tag="PKN91_04975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNS27461.1"
                     /translation="ATLDRFVLVHHRPVAPAGSRPRALQILPLDGAWLGRLAARPWDS
                     RTLPAFTMDWHQLFSSLVRQHLFVALYRAFAESLASENASRLASMQAAEKNIQDHLGR
                     LALDYHQNRQQSITEELLDIVAGFETLTADSLQRIRAAT"
     gene            537..1046
                     /locus_tag="PKN91_04980"
     CDS             537..1046
                     /locus_tag="PKN91_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNS27462.1"
                     /translation="MATSLLLASAAGAHEGKTHASPASAPLAGSALAVAEVLAQYATA
                     LTANDLEAVRPLIADERAFSYFEGSHVDSSWQDYHDHLAPEMALFEQPTYRISDVRPY
                     VVGDLAYATYTWTFDVTVRSAEFEGGRHRVSMTGTGTAVLSQTAGQWRIRHLHTSMAP
                     ARRSGAAAH"
     gene            1157..2620
                     /locus_tag="PKN91_04985"
     CDS             1157..2620
                     /locus_tag="PKN91_04985"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HNS27463.1"
                     /translation="MRSIHFRRPLAALSLLAALVALLPMPRAAMAAQDSGEEGIPAGS
                     VVSLRVADVIGPASADFISRGIDRATRARAALVVIELDTPGGLDTSMRAIIQKILASH
                     VPIATFVYPEGARAASAGTYILYASHIAAMAPATNLGAATPVSIGGGPEAPTGPEKPA
                     GSERGNGAEDVASNAESLRRKAVNDATAYIRGLAELRGRNAEFAERAVREGASLPAEA
                     AKREGVVDYVAHTTRDLLQQLDGATVTTASGSHTLALADAPITALEPGVRTRVLAFIA
                     NPQVAVVLMMIGIYGLFFEFTSPGFGVPGIAGAIFLLLGLYAFHVMPINWAGVALMAL
                     GAVMMLAELFMPSFGALGIGGIIAMLLGGLFLVETEAPGFGLPLTFLLAMTLVSAVAI
                     FGIGTFAARSRARPVVSGREQLLRSSGIVTRVDATSAWAQIEGENWQIQSSAPLAEGM
                     TVHVVGLRGLTLIVEPAASSPSGRPAADDLNHSSEGS"
     gene            2623..3390
                     /locus_tag="PKN91_04990"
     CDS             2623..3390
                     /locus_tag="PKN91_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNS27464.1"
                     /translation="MPLNLLTGYGPIIVVLLILVFSSIKILREYERGVIFLLGRFWKV
                     KGPGLILVVPGIQQMVRVDLRIVTMDVQPQDVISRDNVSVKVSAVIYFRVVDPQRAII
                     QVENFLMATSQLAQTTLRAVLGKHDLDTMLSEREKLNHDIQKILDSQTDGWGIKVANV
                     EIKHVDLNESMVRAIARQAEAERERRAKVIHAEGERQAAQALVEAAEMLVREPAAIQL
                     RYLQTLTQIANERTSTIVFPMPGEFADLLSRVARRDG"
     gene            complement(3505..4809)
                     /locus_tag="PKN91_04995"
     CDS             complement(3505..4809)
                     /locus_tag="PKN91_04995"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HNS27465.1"
                     /translation="MEANRICLAIVVAAFAGNPAAAGPAAATADVVLAWNARLLAAAE
                     AEDGFLTLKGVRTAAMMHVAMHDALNAITPRYAPYAYHGHAPDADPVAAAAQAAFAVA
                     SSQYPGEAPQLRAELERFLAQAAEGPPRRRGISLGESVAHAILARRDGDGWNTEAQYA
                     WQPMGPGVYAEFDEHSGTPKGFVFGAGWAQVRPFLMRSPDQFRSPPPPDVASDAYVAA
                     YDEVREVGRFQSRTRTADETHLALWWKEFVEASHNRLARDLASISGLDPWQAARLFAL
                     LNMAIFDGYIASFDAKFAHNHWRPYTAIRWAANDGNPRTVPEADWNNLHRHTYAFPSY
                     PSAHGTACAAAMGVMANVFGARRGFTMRIPLVDSAGPGSPKIAPDPPTRSFANFDDAA
                     MECALSRVYLGIHFRYDSIQGNRLGSTLAGYGLSHHLVPIGR"
     gene            4917..5708
                     /locus_tag="PKN91_05000"
     CDS             4917..5708
                     /locus_tag="PKN91_05000"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS27466.1"
                     /translation="MRRGYGQYCPLALAAEILCERWTLLIVSRILDGCTQFNQIHRGV
                     PRISPSLLSQRLAGLERAGIIVREPAAGKGLPRRYAPTAAARELEPIIMQIAVWGQNW
                     SRDMTHDDLDPGFLVWSMHVRLDTARMPPGRTVLEFEFTGAPRDCRRFWLVNDDGRVE
                     MCLKHPGFEIDLRVESDLRLFIEAWRGIRDLRAEIAARRVRVLGPPRLCRQFPGWLQL
                     SALAPFPRKRPGREQRLAKAAPRSGSAARSRAGCDAAKRCQRSIQ"
     gene            complement(5690..6520)
                     /locus_tag="PKN91_05005"
     CDS             complement(5690..6520)
                     /locus_tag="PKN91_05005"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNS27467.1"
                     /translation="MASPCEVLCETEDLAFARALGNYAAAEARRIDEKFSRYKAGSVV
                     ADILASRGRSITVDEETSRLLDYGEMLWRVSEGRFDLTSGILRHAWQFEEATDAPRLE
                     RISDLMRHVGWQRLQWKSPRLTLPVGMEIDFGGIGKEYAVDRVADWAARESRDPVLVN
                     FGGDLRAAGAAPASGAWQVGIESLAKPGHAARRLALESGALATSGDARRAIVIDGKRY
                     GHILDARTGWPAPGAPRSVTVAAPTCSQAGSFSTLAMLHGAEAEELLRAEGLRYWILR
                     "
     gene            complement(6564..7859)
                     /locus_tag="PKN91_05010"
     CDS             complement(6564..7859)
                     /locus_tag="PKN91_05010"
                     /inference="COORDINATES: protein motif:HMM:NF023516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HNS27468.1"
                     /translation="MQLKRKPVGATLAAATCSLLGSLPAVAQEAKAWSVDSSLLYYGE
                     DNGRVKDLSLMASIRRALDEDRSLSATLTVDSLTGATPNGAVPAGVPQTFTGPSGGGS
                     YTVAPGAQPLDTTFLDTRVALAANWQQALGTAMRWNVGLSASHEYDYLHLGLNARLER
                     DFNQRNTTVFLGAAYGQDEIKPVGGVPVGLSPMAGEIDDGADRGRAGDDTKDVADILI
                     GVTQVLTRRSLLEVAYSYGQSDGYLTDPYKILSVVDPVSGAPVASTEDGIDFQYLYEH
                     RPDSRTKQSLFAEWRYAFDRDSLALNVRLMDDDWGVQSQTAEARYRWNINERSYLEPH
                     LRWYSQGAADFYRTVLFEGDPLPAFATADYRLGDLDAYTIGAKYGRRTAHGEFSVRLE
                     YYQQSATESPGAAVGALAGFDLTPDLKAVIVQFGYKFDF"
     gene            complement(7850..8047)
                     /locus_tag="PKN91_05015"
     CDS             complement(7850..8047)
                     /locus_tag="PKN91_05015"
                     /inference="COORDINATES: protein motif:HMM:NF025452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HNS27469.1"
                     /translation="MLAAGIACAAALAGCAGVEPWERDLHARADMKADAHDAAIDDHI
                     YFSKEASSGGRSFAGGGCGCN"
     gene            complement(8110..8601)
                     /locus_tag="PKN91_05020"
     CDS             complement(8110..8601)
                     /locus_tag="PKN91_05020"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNS27470.1"
                     /translation="MESLVRIATLILTLLAAVGTASADTAAQASAAAAPRWSVLDAYA
                     GKVVLVDFWASWCTPCLRSFPWMNELQQQLGSEGLVVVAVNLDQERALADAFLGKNPA
                     RFRVEFDAAGTIARQFGVQAMPTSFLIDRNGKLRARHTGFKEKLRASREQQIVQLLKE
                     PAT"
     gene            complement(8666..10546)
                     /locus_tag="PKN91_05025"
     CDS             complement(8666..10546)
                     /locus_tag="PKN91_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS27471.1"
                     /translation="MGQEVTRRDFLDGAALAIIAGLSPLELLRAAAPMPGADHPPARH
                     GLRGSAPGSFDVAHALRDGADYPIGDVAIDEQYDLVVIGAGISGLAAAWFYRRQKPNA
                     RILLLDAHDDFGGHAQRNEFDVDGRRLIAYGGSESLQSPQSLYSREALGLLRALGVDI
                     HRFETAFDRSLYPGLGLSRGVLFKRERFGVDRLVTGDPLRMVADDIPPDRLNARPVEA
                     FIADFPVSGDAKRELIELYTSRRDPLAGRSRADKEALLERTSYRDWITKVWGFSDEVA
                     GTFAGRPLDFFALGIDALPASWARETGYPGFAGLGLPTDDIEAEMSDPYIHHFPDGNA
                     SIARLLVRSLIAGVAPGRDMEDIVFAPFDYARLDSAGALVRLRLQSTVVRLRNRGDGV
                     DAFYVRNGQLRGARSRHAIFAAYHSMAPWVLPDLPAAQRRALALNVKAPLVYVNVAVR
                     NWRPWVRRGVHEVTNTTGFYSRIKLDYPVSLGGYRCPRSPDEPMVLHLVHVPTVPLSP
                     GHDLRAALRASRTGLLAMPFERFEAEARDELTRIVGAGGFDADRDIAAITVNRWGHGY
                     SGGDNPLFDAKRDGAAPNETARARFGAIAFANSDSAWQAYAHAAIDEAHRAVVEILGT
                     PA"
     gene            complement(10631..12709)
                     /locus_tag="PKN91_05030"
     CDS             complement(10631..12709)
                     /locus_tag="PKN91_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNS27472.1"
                     /translation="MKRPRHLLLNTLMLAIGAGATSFAVAQSDSGKDVYLQNCAACHQ
                     PDGKGLAGAFPPLAGSDWLKGKSAADVAATVLTGLQGELVVNGVTYNSVMPAQSHLSD
                     AEIAAAVTYILNNWGNAGGRVTAAEVTAQRTKLAVTTDPAQGELHPGTSQAAAAYEGA
                     PVAASGADVKQVRTPGAPDMSEAEFASASEIFFQRCAGCHGVLRKGATGKPLTPDLTQ
                     ARGTEYLKALIKFGSPGGMPNFGTGGVLSEDQVGLMARFLQHTPPNPPEWGMKEMRDS
                     WTVFIPPEKRPTRKLNNYNLDNVFAVTLRDSGEIALIDGDTKKIINILKTGYAVHISR
                     VSHSGRYIYTIGRDGKIDLVDLWMEVPDRVAEIKIGLEARSVETSKFKGFEDKLAIAG
                     AYWPPQYVIMDGPTLEPMKIVSTRGMTVDTQEYHPEPRVAAIVASHKHPEFIVNVKET
                     GRILLVDYSDIDSLKTTTLDAARFLHDGGWDSSKRYFLTAANQSDKIAVVDSLEQKMV
                     GLIDVDKIPHPGRGANFVHPKYGPVWATSALGNENITLVATDPEKHPQFAWKAVEVLK
                     GQGGGSLFVKTHPKSRHLWVDTTLNPDAKISQSIAVFDINNLDKGYKVLPIAEWAKLG
                     DGPKRVVQPEYNKDGTEVWFSVWNGKEQRSAIVVVDDATLKLKAVIDDPRLITPTGKF
                     NVYNTVNDVY"
     gene            12904..15123
                     /locus_tag="PKN91_05035"
     CDS             12904..15123
                     /locus_tag="PKN91_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HNS27473.1"
                     /translation="MLVRLLPFALLPGIAVGQSGVEVSAEGADSMALDTVVIVASRVA
                     EPLSQVVASVAQVERADLDRHLVRDLEGLTRYVPGVDVTRDSHRFGSNGFSIRGLEGN
                     RVRILIDDIPLADAYSIGQFASAGRDLVDLEAIERVEVLRGPASTLYGSDALAGVVAF
                     RTRNPEDLLAPGDNRHFGLRMGWDSVDDSLLGAASWAGESASGRWQGMIVLGQRSGHE
                     ADNRAWRDEDGPNSIDYRRDSALAKLVHDAGNAGRYVLTLEGNRSERQTAVNSLRFGS
                     GRFSTTYRLDADDRDRRNRASLAARWEPQRPWLQSLEAQLYVQDTSVRQDSDQYRLPD
                     RATPFESLRWRRFEYDASAIGLGLLGQSRHEGRWARHWQVFGIDLTTQRYEGLRDGLE
                     TNLATGDSSSLILGEPLPVRDFPNTDADSLALYWQDEISLGERFALIPGLRAEWYRLR
                     AKPDAIFREDFPDATPVDIDEQQLTPRLALRWSPGGGHSLFVQYARGFRAPPFGDVNI
                     GLILPVFNYEVRANPDLRPERSQGLELGWRHVGDTLRASVSLYENRYRDLIESRANLG
                     IEPDTGLLVFQSINRDRARIRGVEADLLWNLPGRPDGAGHWQLRGALAWAQGDDLRRG
                     QPLNSIDPARIALGLRYEALSDRWGMEAAMVGTQAKDRIDHENGPYFAPPGYVRFDAY
                     AWAEPWRGVRINVGVVNIGDRRYWDWSGVRGVGANDANIGFYSRPGRSLAVKLAIDW"
     gene            15370..>20897
                     /locus_tag="PKN91_05040"
     CDS             15370..>20897
                     /locus_tag="PKN91_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27474.1"
                     /translation="MKARRGGAKRFRRTFGALAAICLAAAGLARADQTVDVTVIPGTE
                     GDDTIAVLTPEQVDALIASIIAEISKASATLLGSLAGNDTVTIDADLDAFSLVGTLIG
                     SNPGESASSATSITVATGSGVDFVDAAGVFSSTADSTSAYADVLPVDDEFLPTGSQKA
                     DVSIKATSQATGLDTGADNDVVIASAELSSSAAAITGGVAAGIQASFSNSVSTKAGSV
                     AESKAGGIQADAGNDQVAHRGTIVASATAASGVIAVEVNASDENDAERKVQGKLDASA
                     KATAGSVGIDTDGASTESDLDVTLGLPDDAIQVTVEWSETLESGADTVANLGAVTSTA
                     SAISDSVAVGVSAAAKGSLKATINAEAKAASRGINSGAGDDAVTNAGSIGAAADAAAW
                     VVGATISAGQGADENKAAKGKAHTSTNAIAESRATGIDADGPAASSSSTVDTVIGRSG
                     ISTTYLTSETAATGVDDVVNTGTVTVNSSALAGSRVVTVQVNAEGTAQSEANSTADSG
                     GIGISAGGGDDAVDNRGVLTVGADATAGALAVTFDQGGEKAESKVDAKVEAAAVATGI
                     AADGGAVDITTEISMTIDADGLLAERNRQQTTQHGDDTILNSDAVGATATALAGAAGA
                     AVAIDGSASSDMTSKAGATAAGIDGGGGNDLVDNEGELTASADASAGALSIGFAQKVE
                     EKAEAEVEVKATVEAEATATGLAGDSGRDVVAEYRLAIGADGLDLTLLNNAGALSGDD
                     QLVNRGAITATANSLTGAAGVSVTIDGASAAELESKAKSHASGIDAGGGNDLVESAGS
                     ATAEASSTAGALGVAFAQKPGPKENAKAEVSAEAGATATGIAGDSGALVVESLELTLG
                     GAGLDSSASRERTANRGDDVILATGDVTASAYATSATLGVTVAIDGAAEAEMKATAEA
                     KAAGIQGGGGNDRVESSGLVTTLADSSAGGVAAAVGQQSSDGSKAKTKVSAETESVAS
                     ATGIAGDGGQEDSVETVAVNIGSSGLSFGYEKTAIAASGDDELLNTGDVDTTAVADTA
                     AAAVAVTVKGGAKADVDAKAEATARGLDAGGGADNITNSGTLTTDATATALTANVSVS
                     TEGSSMANAGFLSGDNEVEAEAVGISSAGSDHQTSTVITTDIDFTDFAVTGSYEKIED
                     HVSGDGADRIFNSGEVTSDSFATTLAADVGVTGEGSALALGRAKVEAHAGGIESGNGD
                     DTVENTGALTSNATATAALANVAVTGKGLAVAGNSAWDGGTHAEASAAGIDADSGEIE
                     ATTVTFEASLERAQVVYEGTLEAATGNDVVLNEGDILAQAISTAATASVAVSPKGMSA
                     AVSTSSAESETFGIKGGGGDDRIENTGLLTSYADSLAATASVSVTSGPGVAVAADAVW
                     DSGTKAEAKAIGIAGDGGNQSESTRLAIGTDEMGLDKDEVVADGADTILNFGDMDVDA
                     IATTASASVAVTASSGVAAATATSTAEATAAGIDAGAGTGVDQVYNAGILDVAADAAA
                     ASASVSVVNTGVAIAADSVWDGGTTATARARGIDVGAGGDTIENDGETTATATALAGS
                     ATVAVTSTGFAGASGTSTGTADASALDASAGIDSDTIINRGALNAAAEAAAATANVTF
                     TNTGAALALGAVWDGGTGATARARGIETGDGADGVVSTGTIAATADALAAEAAVSVTA
                     TGVAGAVATATAETDAVAIDLGGDAVADTVDNQGELTVDSNALAFSAAVSATSTGVAV
                     SGGDVWDGGTQATARARGIETGGGADGILNTGAIGLDSDAVTAAASVSVALTGVGGAI
                     SRSTSVSDATAIDAGDDAGDDTVENSGDLTVNSDAFAAAVSAAFTSAGHRRPHFLSWK
                     A"
BASE COUNT         3409 a         6961 c         7290 g         3237 t
ORIGIN      
        1 aagcgaccct cgaccgcttc gtcctggtcc accacaggcc ggtcgccccc gcgggctcac
       61 ggccacgcgc gctgcagatt cttcccctcg atggggcctg gcttggccgg ctggctgcac
      121 gaccctggga ttcgcgcacg ctgcccgcgt tcacgatgga ctggcaccag ctgttctcct
      181 cgctggtccg ccagcacctg ttcgtcgcac tctaccgcgc attcgccgag tcgctggcga
      241 gcgaaaacgc cagccgcctc gcttccatgc aggccgccga gaagaacatc caggaccacc
      301 tgggccgcct tgcgctcgac taccaccaga atcgccagca gtcgatcacg gaagaactcc
      361 tcgacatcgt ggccggcttc gagaccctga ccgcggacag tctgcagcgg atcagggcag
      421 caacgtagac actatgcatc aggaacgcct gtcctgccca tgaaccgcgg ccaccataca
      481 gaccggagaa cacgatgagc cctcgattgc cagctgcccg ccgaattgcc gccctcttgg
      541 ctacctcgct gctgctcgcc agcgccgcag gcgctcacga aggcaagacg cacgcatctc
      601 cggccagcgc accacttgcc ggcagcgcgc ttgcggtcgc cgaagtactc gcccagtacg
      661 ccaccgcgct caccgccaat gatctcgagg ctgtgcggcc gctcatcgcc gatgaacgcg
      721 ccttcagcta cttcgaaggc agccacgtcg acagcagctg gcaggactac cacgaccatc
      781 tcgcgccgga gatggcgctg ttcgagcagc cgacctatcg catcagcgac gtgcgtccct
      841 atgtagtcgg cgacctcgcg tatgccacct atacctggac attcgacgtg acggtgcgca
      901 gtgccgagtt cgagggtggc cggcatcgtg tttcgatgac cggcacgggc acagccgtgc
      961 tcagccagac tgcagggcag tggcgcattc gccacctgca cacatccatg gcgcccgcgc
     1021 gcaggtcggg tgccgccgcg cattgaggcg tcgccccgtg attcgatctg ctcgtgcgcg
     1081 gtgctgcggc gcatgcattc ggtacacact gcgccgagga tgtggctatg atcgatcgaa
     1141 cgccaaggag ctggaaatgc gatcgatcca tttccgcagg ccgttggcag ccctctcgct
     1201 gcttgcggcg ctcgtcgcac tgctgccgat gccgcgtgcc gcaatggcag cacaggattc
     1261 gggcgaggag ggcattcccg ccggaagcgt cgtcagcctg cgtgtcgctg acgtcatcgg
     1321 gcccgcctcg gccgatttca tcagtcgcgg catcgatcgc gccacccggg cccgggcggc
     1381 cctcgtcgtc atcgagctcg atacaccggg cggcctggat acctcgatgc gcgcgatcat
     1441 ccagaagatc ctggcgtcgc acgtgccgat cgcaacgttc gtctatcccg agggcgcgag
     1501 agccgcgagc gccggcacgt acattctcta cgccagccat atcgcggcca tggcgcccgc
     1561 gacgaatctc ggcgcagcaa cgcccgtctc gatcggcggc ggccccgagg caccgaccgg
     1621 gcccgagaaa ccggccgggt ccgagcgcgg caatggggcc gaagatgtcg catccaacgc
     1681 cgaatcgctg cggcgaaagg ccgtcaacga tgccacagcc tacattcgcg ggctggccga
     1741 gctgcgcggt cgcaatgcgg agttcgccga acgggccgtg cgcgaaggcg cgagcctccc
     1801 ggccgaggcc gcgaagcgcg agggcgtcgt cgactacgtc gcgcatacga cacgcgacct
     1861 tctgcagcag ctcgacgggg caacggtaac gaccgcatcg ggctcgcaca ccctcgccct
     1921 cgcggacgcg cccatcacgg cgctcgaacc gggcgtgcgc acgcgcgtcc ttgctttcat
     1981 agccaatccg caggtggccg tcgtgctcat gatgatcggc atctacggcc tgttcttcga
     2041 gttcacgagt ccgggcttcg gtgtgcccgg catcgcgggc gccatcttcc tgctgctcgg
     2101 tctctatgcg ttccacgtga tgccgatcaa ctgggcaggt gtcgcgctca tggcactcgg
     2161 cgctgtcatg atgctcgccg agctcttcat gccgagtttc ggtgcgctcg gcatcggcgg
     2221 catcatcgcg atgctgctcg gcgggctgtt cctcgtcgag accgaagcgc cgggcttcgg
     2281 gttgccgctg acgttcctgc tcgcgatgac gctcgtcagc gccgtcgcca tcttcggtat
     2341 cggcaccttt gcggcaagga gcagggcgcg accggtcgtg agcgggcgcg agcagctcct
     2401 gcgcagcagc ggcatcgtca cccgcgtcga cgccacatcc gcctgggcgc agatcgaagg
     2461 cgaaaactgg cagatccagt catcggcgcc tctggctgaa ggcatgaccg tgcacgtcgt
     2521 cggactccgg ggactcacgc tgatcgtcga gcccgcggcc tcgagcccgt cggggcgccc
     2581 ggccgcggac gacctcaacc attcttcaga ggggagctga ccatgccact caatctgctg
     2641 accggctacg ggccgattat cgtcgtcctc ctcatactcg tattttcgtc gatcaagatc
     2701 ctgcgcgagt acgagcgcgg cgtcatcttc ctgctgggtc gcttctggaa ggtcaaaggc
     2761 ccgggtctca tcctcgtcgt gccgggcatc cagcagatgg tgcgcgtcga cctgcgcatc
     2821 gtcacgatgg atgtccagcc gcaggacgtg atctctcgcg acaacgtctc ggtcaaggtc
     2881 agcgcggtca tctatttccg tgtcgtcgat ccgcagcgcg cgatcatcca ggtcgagaat
     2941 ttcctcatgg cgacgagcca gctcgcgcag acgacactgc gcgccgtgct cggcaagcac
     3001 gatctcgaca cgatgctgtc cgagcgcgag aagctcaatc acgacatcca gaagatcctc
     3061 gactcgcaga ccgacggctg gggcatcaag gtcgcgaacg tcgagatcaa gcatgtcgat
     3121 ctcaatgaaa gcatggtgcg agcgatcgcg cgccaggccg aggccgaacg cgagcggcgt
     3181 gccaaggtca tccacgccga aggcgagcga caagcggccc aggcgctcgt cgaagcggcc
     3241 gagatgcttg tgcgcgagcc ggccgccatc cagctccgtt acctgcagac actcacgcag
     3301 atcgcgaacg agcgcacatc gacgatcgtc ttcccgatgc cgggcgaatt cgcggacctg
     3361 ctgtcgaggg tggcgcggcg cgacggctga cgcgggcgcc gccgcgaacg gagtgcgtcg
     3421 cactgacggc gcctggccgc gtgcgggcgc ggacggtcat ggtgggcttg cagccggcga
     3481 tgccctgacc tccgcacagg gcggctagcg cccgatcggc acgaggtgat gcgagaggcc
     3541 gtatccggcg agtgtggacc cgagccggtt gccctggatc gagtcgtagc ggaagtggat
     3601 gccgaggtag acacgcgaca gcgcgcactc catcgcggcg tcatcgaagt tcgcgaacga
     3661 acgggtcggt gggtcgggtg cgatcttcgg tgaccccggg cccgcagagt cgacgagcgg
     3721 gatccgcatc gtgaagcccc gccgcgcacc gaacacattc gccatcacgc ccatcgccgc
     3781 cgcgcacgcc gtgccgtggg ccgacggata cgacgggaac gcgtaagtgt gccggtgcag
     3841 gttgttccag tcagcttccg gtacggtgcg cggattaccg tcgttcgccg cccaacggat
     3901 cgccgtatag ggtcgccagt ggttatgggc gaacttggcg tcgaaggacg cgatatagcc
     3961 gtcgaagatc gccatgttga gcagcgcaaa gagccgcgcc gcctgccacg gatcgagacc
     4021 ggagatcgag gcgagatcgc gcgcgaggcg gttgtgtgag gcctcgacga actccttcca
     4081 ccacagggcg agatgcgtct catcggcggt gcgcgtccgg ctttggaaac gtccgacttc
     4141 cctgacctcg tcataggcgg cgacgtacgc gtcgctcgcg acatcgggcg gcgggggtga
     4201 gcggaactga tcgggcgagc gcatcaggaa cggtcgcacc tgtgcccagc cggcgccgaa
     4261 gacaaaaccc ttgggcgtgc cgctgtgttc atcgaactcc gcgtacacgc cgggccccat
     4321 gggctgccag gcgtactgcg cttcggtgtt ccaaccgtca ccgtcgcgcc gcgcgaggat
     4381 tgcgtgcgcg acggactcgc cgagcgagat gccgcgcctc ctcggtggtc cttccgcggc
     4441 ctgcgcgaga aagcgctcga gttcggcccg cagttggggc gcttcgccgg ggtactggct
     4501 cgaggccaca gcaaatgccg cctgcgccgc agcggcgacc gggtcggcat ccggcgcgtg
     4561 tccgtggtac gcgtacggcg cataccgcgg cgtgattgcg ttgagcgcgt catgcatcgc
     4621 aacatgcatc atcgcggcgg tgcgcacgcc cttgagcgtc aggaagccgt cctccgcctc
     4681 ggcggccgcg agcagccgcg cgttccaggc taagacgacg tccgcagtcg cggcggcagg
     4741 gcccgccgcc gccggattgc cggcgaacgc cgcgaccaca atcgcaaggc agatccggtt
     4801 cgcttccatg caggcgtcct gccctcggga tcagggccaa tctagtcgtc cggcaggacg
     4861 ccggctacta cgacttctgt agtgactcgg gggcgcatcg ctgggagaat tgccgcatga
     4921 ggcgcggcta cggtcagtac tgcccgcttg cgctggcggc ggagatcctg tgcgagcgct
     4981 ggaccctcct catcgtcagc cggatcctcg acggctgcac gcagttcaac cagatccatc
     5041 gcggcgtgcc gcgcatttcc ccgtcacttc tctcgcagcg gctcgccggg ctcgagcgtg
     5101 ccggcatcat cgtgcgcgag ccggcggcgg ggaaaggact cccgcgccgg tatgcgccga
     5161 ccgcggcggc acgcgagctc gagccgatca tcatgcagat cgcggtatgg ggccagaact
     5221 ggagccgcga catgacgcac gacgacctcg acccggggtt cctggtgtgg agcatgcacg
     5281 ttcggctcga caccgcccgc atgccgccgg gccgcaccgt gctcgagttc gagttcacgg
     5341 gcgcgccgcg ggactgccgc cgcttctggc tcgtcaatga cgacggccgg gtcgagatgt
     5401 gcctcaagca tcccggcttc gagatcgacc tgcgcgtcga gtcggacctc aggcttttca
     5461 tcgaggcgtg gcggggaatc cgcgacctgc gcgcggagat cgcggctcgg cgggtccgcg
     5521 tgctgggacc gccgcgcctg tgccggcagt tcccgggatg gctgcaactc agcgcgctgg
     5581 ctccgtttcc gcgcaaacgg cccggccggg agcagcgcct cgcgaaggca gcgccgcgca
     5641 gcgggagcgc cgcgcgcagt cgggccggat gcgacgcggc aaagaggtgt cagcgaagta
     5701 tccaatagcg caagccctcg gctcgcagca actcttcggc ttcggcaccg tggagcatgg
     5761 cgagtgtcga gaagctgccg gcctgcgaac aggtgggcgc ggcgacagtg acggagcgcg
     5821 gcgcaccggg ggccggccag ccggtgcggg cgtcgagaat atggccgtag cgcttgccgt
     5881 cgatcacgat cgcgcggcgc gcgtcgccgc tggtcgcgag cgcgcccgac tcgagcgcga
     5941 ggcgccgcgc cgcgtgcccg ggcttcgcga gcgattcgat ccccacctgc caggcgccgc
     6001 tcgcgggcgc agcgcctgcc gcgcgcaggt cgccgccgaa gttgacgagt accggatcac
     6061 gcgactcgcg cgcggcccag tcggcgacgc ggtcgaccgc gtattccttg ccgatgccgc
     6121 cgaagtcgat ctccatgccg accggcagcg tcagccgggg tgatttccac tgcaggcgct
     6181 gccatccgac atggcgcatc agatcggaga tgcgctccag ccgtggtgcg tcggtcgcct
     6241 cctcgaactg ccaggcgtgg cgcaggatgc cggacgtgag gtcgaagcgg ccctcgctca
     6301 cgcgccagag catctcgccg tagtcgagga gccttgaggt ttcctcgtcc acggtgatcg
     6361 agcggcccct gctcgcgagg atgtcggcga cgacgctccc cgccttgtag cggctgaatt
     6421 tctcgtcgat gcggcgcgcc tcggcggccg cgtagttccc gagcgcgcgc gcaaaggcga
     6481 ggtcctccgt ctcgcagagc acctcacagg ggctcgccat ggcgctgaat ccgatgcgcc
     6541 atagccgctg tacgcgggtc aggtcaaaag tcgaacttgt atccaaattg cacgatcacg
     6601 gccttcaggt cgggcgtgag gtcgaagccc gcgagcgcgc ccaccgccgc gccgggcgac
     6661 tcggtcgccg actgctgata gtactcgagc cgcacggaga actcgccgtg agcggtacgc
     6721 cggccgtact tcgcgccgat cgtgtacgcg tcgaggtcgc cgagccggta gtcggcggtg
     6781 gcgaacgccg gcaacgggtc accctcgaac agcacggtgc ggtagaaatc tgcggcgccc
     6841 tggctgtacc agcgcaggtg cggctcgagg taggagcgct cgttgatgtt ccagcgatag
     6901 cgcgcctccg ccgtctgcga ttgcacgccc cagtcatcgt ccatgagccg cacattgagc
     6961 gcgagggaat cgcggtcgaa cgcgtagcgc cactcggcga acaggctctg cttggtgcgc
     7021 gagtccgggc gatgttcgta caggtactgg aagtcgatgc cgtcctccgt cgaagcgacc
     7081 ggcgcgccgc tcacgggatc gactaccgag aggatcttgt agggatcggt gaggtagccg
     7141 tcgctctgac cgtaggagta cgcgacctcg agcagtgagc ggcgcgtgag cacctgcgtg
     7201 acgccgatca ggatatccgc gacgtccttc gtgtcgtcgc cggcgcggcc gcgatccgcg
     7261 ccgtcgtcga tctcgcccgc catcggcgag aggccgaccg gcactccgcc taccggcttg
     7321 atctcatcct ggccgtaggc ggcgccgagg aagacggtgg tattgcgctg gttgaagtcg
     7381 cgctcgaggc gcgcgttgag tccgaggtgc aggtaatcat actcatgcga ggcactgagg
     7441 ccgacgttcc agcgcatggc ggtgccgagt gcctgctgcc agttcgccgc aagcgcgaca
     7501 cgcgtgtcga gaaaggtcgt atcgaggggc tgcgcgcccg gtgcgaccgt gtagctgccg
     7561 ccgccggaag ggccggtgaa ggtctgcggt acgcccgccg gcactgcgcc gttcggcgtc
     7621 gcgccggtca gcgagtcgac ggtgagcgtc gcgctgaggc tgcggtcctc gtcgagcgca
     7681 cggcggatgg acgccatgag gctcagatcc ttgacgcggc cgttgtcctc gccgtagtag
     7741 agaagcgagc tgtcgacgct ccaggccttg gcctcctgcg ccaccgccgg cagcgagccg
     7801 aggaggctgc aggtggcggc cgcgagcgtc gctccgaccg gtttgcgctt cagttgcatc
     7861 cgcagcctcc gcccgcaaag ctgcgcccgc cgctgctcgc ctccttgctg aaatagatgt
     7921 ggtcgtcgat ggcggcgtcg tgggcgtcgg ccttcatgtc ggcacgggca tggaggtccc
     7981 gctcccaggg ctcgacgccg gcgcagccag ccagggcagc ggcgcaggcg atgcctgccg
     8041 ccagcatgac ggcggcgagg ctgcgactgg atcgcgatcc ggcgcaaggg ctcatttggc
     8101 gtgtgcaact catgtggcgg gttccttcag aagctggacg atctgttgtt cgcggctcgc
     8161 gcggagcttt tccttgaagc cggtgtggcg ggcgcgcagc ttgccgttgc ggtcgatcag
     8221 gaagctggtc ggcatggcct ggacgccgaa ctggcgagcg atcgtcccgg cggcgtcgaa
     8281 ctcgacgcgg aacctggccg ggttcttgcc gagaaaggcg tcggcgagcg cgcgctcctg
     8341 gtcgaggttg acggcgacga cgaccagtcc ctcgctgccg agctgttgct gcagctcgtt
     8401 catccacggg aaggaccgca ggcagggcgt gcaccaggag gcccagaaat cgacgaggac
     8461 gaccttcccc gcgtaggcgt cgagcaccga ccagcgcggc gcggccgcag cggaagcttg
     8521 cgcggccgtg tcggcgctgg cggtgcccac ggctgcaagc agggtcaaga tgagggttgc
     8581 gatccgcacc aaactctcca ttcagacgat ggcagccgtt ggacggttgc tgcgggcaaa
     8641 ttcaatcgcg gttggcgcag cgcgctcagg cgggcgtgcc aaggatctcc acgaccgcgc
     8701 gatgcgcctc gtcgattgcg gcatgcgcgt aggcctgcca ggccgagtcg gagttcgcga
     8761 acgcgatggc gccgaagcgc gcgcgggcgg tctcgttcgg tgcagcgccg tcgcgcttcg
     8821 cgtcgaacaa cgggttgtcg ccgcccgagt agccgtggcc ccagcgattc acggtgatcg
     8881 cggcgatgtc gcggtcggca tcgaagccgc cggcgccgac gatgcgcgtc agctcgtcgc
     8941 gcgcctcggc ctcgaagcgc tcgaaaggca tcgcgagcag tccggtgcgc gaggcgcgca
     9001 gtgcggcccg caggtcgtgc cccggggaga gcggaacggt cggcacgtgc acgagatgca
     9061 gcaccattgg ctcatcgggt gatcgtgggc agcggtagcc gccgaggctc accgggtaat
     9121 ccagcttgat gcgcgagtag aagccggtcg tgttggtcac ttcgtgcacg ccgcgcctga
     9181 cccatggccg ccagttgcgc acggcgacgt tcacatagac gagcggcgcc tttacattga
     9241 gcgcaagcgc gcgccgctgc gcggcgggaa ggtcgggcag cacccagggc gccatcgaat
     9301 ggtaggcggc gaagatcgcg tggcgggagc gtgcaccgcg caactgtccg ttccgtacgt
     9361 agaaggcatc gacaccatcg ccgcgattgc gcaggcgcac gacggtgctc tgcaggcgca
     9421 ggcgcacgag cgcaccagcg gaatcgagcc gcgcgtagtc gaacggcgcg aacacgatgt
     9481 cttccatgtc gcgacccggc gcgacacccg cgatcagcga acgcaccagc aggcgtgcga
     9541 tcgaagcgtt gccgtcgggg aagtggtgga tgtacggatc gctcatttcg gcttcgatgt
     9601 catcggtcgg caagccgagg cccgcgaagc cgggatagcc cgtctcgcgc gcccatgaag
     9661 cgggcagggc gtcgatgccc agggcgaaga agtcgagcgg gcggccggca aaggtgcccg
     9721 ccacttcatc cgaaaacccc cacacttttg tgatccagtc gcgatagctc gtgcgttcga
     9781 gcagcgcctc cttgtccgcg cgcgagcggc ccgcgagcgg gtcgcgccgc gaggtgtaga
     9841 gctcgatcag ctcgcgcttt gcatcgccag agaccgggaa gtcggcgatg aacgcctcca
     9901 ccgggcgagc attcagccga tcgggcggga tgtcgtcggc gaccatgcgc agggggtcgc
     9961 cggtgaccag gcggtcgact ccgaagcgct cgcgcttgaa gagcacgccg cgcgacaggc
    10021 cgagcccggg atagaggctg cgatcgaagg cggtctcgaa gcggtggatg tcgacaccca
    10081 gcgcgcgcag cagtccgagt gcttcgcggc tgtagagcga ttgtggcgat tgcagcgatt
    10141 cgctgccgcc ataggcgatc agccggcggc catcgacatc gaactcgttg cgctgtgcgt
    10201 gaccgccgaa gtcgtcatgg gcatcgagca gcaggatgcg cgcgttcggc ttctggcggc
    10261 ggtagaacca cgcggcggcc agcccgctga ttccagcgcc gatgaccacg aggtcgtact
    10321 gctcgtcgat cgcgacgtcg ccgatgggat agtccgcgcc gtcgcggagg gcgtgcgcga
    10381 cgtcgaagct gccgggcgca gagccgcgca agccgtggcg ggcgggcggg tggtccgcgc
    10441 cgggcatggg ggcggcggcg cgcagcagtt cgaggggtga aaggccggcg atgatcgcga
    10501 gcgcggcgcc gtcgaggaaa tcccgtcggg tgacctcctg tcccatggag gccattctag
    10561 cgcccgaaac gaaccggccg ggatccactg gatcccggcc ggcgagtttc cggacgattg
    10621 cccggtcagc tcaatacacg tcgttgacgg tgttgtagac gttgaacttg ccggtcggcg
    10681 tgatcaggcg cggatcatcg atgaccgcct tgagcttgag cgtggcatcg tcgaccacca
    10741 cgatcgccga gcgctgctcc ttgccgttcc acaccgagaa ccagacttcg gtaccgtcct
    10801 tgttgtactc cggctgcacc acgcgcttcg gtccatcgcc gagcttcgcc cattcggcga
    10861 tcggcagcac cttgtagccc ttgtcgagat tgttgatgtc gaagaccgca atcgactgac
    10921 tgatcttggc atcggggttg agcgtggtgt cgacccacag gtggcgcgac ttcggatggg
    10981 tcttgacgaa cagcgagccg ccgccctgtc ccttcaatac ctcgaccgcc ttccaggcga
    11041 actgcgggtg cttctcggga tcggtggcga ccagcgtgat gttctcgttg cccagcgccg
    11101 aggtcgccca gacagggccg tacttcgggt gcacgaagtt cgcgccgcgg cccgggtgcg
    11161 ggatcttgtc cacgtcgatc aggccgacca tcttctgctc gagcgagtcg accacggcga
    11221 tcttgtcgct ctggttggcg gccgtcagga agtagcgctt gctgctgtcc cagccgccgt
    11281 catgcaggaa gcgcgcggcg tcgagcgtgg tcgtcttcag gctgtcgatg tcgctgtagt
    11341 ccaccagcag gatgcgcccg gtctccttga cgttgacgat gaactcgggg tgcttgtggc
    11401 tggcgacgat cgcggccacg cgcggctccg ggtggtactc ctgcgtgtcc acggtcatgc
    11461 cgcgggtgga gacgatcttc atcggctcca gggtcgggcc gtccatgatc acgtactgcg
    11521 gcggccagta ggcgccggcg atcgccagct tgtcctcgaa gcccttgaac ttggatgtct
    11581 cgaccgagcg cgcctcgagg ccgatcttga tctcggcgac gcgatcggga acctccatcc
    11641 acaggtccac cagatcgatc ttgccgtcgc gaccgatcgt gtagatgtag cggcccgaat
    11701 gcgagacgcg cgagatgtgc accgcgtagc cggtcttgag gatattgatg atcttcttcg
    11761 tgtcgccgtc gatcagcgcg atctcgccgg agtcgcgcag cgtcaccgcg aagacgttgt
    11821 cgaggttgta attgttcagt ttgcgcgtcg ggcgcttctc gggcggaatg aataccgtcc
    11881 agctgtcgcg catttccttc atgccccatt ccggcgggtt ggggggcgtg tgctgcagga
    11941 accgcgccat caggcccacc tggtcctcgc tcagcacgcc gccggtgccg aaattgggca
    12001 tgccgccggg cgaaccgaac ttgatcaggg ccttcaggta ttcggtgccg cgcgcctggg
    12061 tcagatccgg tgtcagcggc ttgccggttg cgcccttgcg cagcacgcca tggcaaccgg
    12121 cgcagcgctg gaagaatatc tcgctcgcgc tggcgaattc ggcttcgctc atgtcgggcg
    12181 cgcccggcgt gcggacctgc ttcacgtcgg caccgctggc cgcgaccggc gcgccttcgt
    12241 aggcggccgc agcctggctg gtgcccggat gcaattcgcc ctgggccggg tcggtggtca
    12301 ccgccagctt cgtacgttgc gcggtcactt cggcggccgt gacgcggccg ccggcattgc
    12361 cccagttgtt gaggatgtag gtgaccgcag cggcgatctc ggcgtcgctc aaatgagatt
    12421 gcgcgggcat gacgctgttg taggtcacgc cgttgaccac caactcgcct tgcaggcccg
    12481 tgagcacggt cgcggcgaca tcggcggcac tcttgccctt gagccagtcg gagccggcga
    12541 gcggcggaaa ggcgccggcc agccccttgc cgtcgggctg gtggcaggcg gcgcagttct
    12601 gcaggtagac atccttaccg gaatcgctct gcgcgacggc gaacgatgtc gcgccagcgc
    12661 cgatggcgag catcagtgtg ttcagcagta agtggcgtgg ccgtttcatg gcggatacct
    12721 gctccaagtg aagtaaaata caagagtgga tcgtccttgc ccggagtatt ccctgtccga
    12781 atgcatcgct ccctgatcta catcaaccga acagtagcgg tgcgaccgtt gatttgcatc
    12841 aggggaatcc ggtcctgcac cagggaaaaa tcggcaatca cgtatatcgc ctggtgcctc
    12901 gccatgcttg ttcgcctgct gccgttcgca ctccttcccg gcatcgccgt cggccagtcc
    12961 ggcgtcgagg tttcggccga gggcgccgac tccatggcgc tcgacaccgt cgtcatcgta
    13021 gcgagccgcg ttgccgagcc gctcagccag gtcgtcgcca gcgtcgctca ggtcgagcgc
    13081 gcggatctgg atcgacatct ggtgcgcgat ctcgaaggcc tgacccgcta tgtgccgggc
    13141 gtcgacgtca cgcgcgacag ccatcgcttc ggcagcaacg gtttctccat ccgtggcctt
    13201 gaaggcaatc gcgtccgcat attgattgat gacattccgc tcgcggatgc gtacagcatc
    13261 ggccaattcg cctcggcggg ccgcgacctg gtcgacctcg aggcaatcga gcgcgtcgaa
    13321 gtgctgcgcg gccctgcatc cacgctgtac ggcagcgatg cgctggccgg cgtcgtggcc
    13381 ttccgcaccc gtaatccgga ggacctgctc gcgcccggcg acaaccgcca cttcgggctg
    13441 cgcatgggtt gggacagtgt ggatgacagc ctgctgggtg cagcgagctg ggcgggcgag
    13501 agcgcttcgg gccgctggca aggcatgatc gtgctcgggc agcgcagcgg ccatgaagcc
    13561 gacaaccgcg catggcgcga cgaggacggc cccaactcca tcgactaccg gcgcgacagc
    13621 gccctcgcca agctggtgca cgacgccggc aatgccggtc gctatgtgct gaccctggag
    13681 ggcaatcgca gcgagcgcca gaccgcggtc aactcgttgc gcttcggcag cgggcgcttc
    13741 agcaccactt atcgcctcga tgccgacgat cgtgatcgac gcaatcgcgc gagtctcgcc
    13801 gcgcgctggg agccgcagcg cccgtggctg cagtcgctgg aagcgcagct atacgtgcag
    13861 gacacttcgg tgcgccagga cagcgaccag taccggttgc ccgaccgtgc cacgccattc
    13921 gaatcgctgc gctggcgacg cttcgaatac gacgcgagcg caatcggcct cgggctgctc
    13981 ggccagtcgc gtcacgaggg ccgctgggcg cggcactggc aggtcttcgg catcgatctc
    14041 accacgcagc gttacgaggg tctgcgcgac gggctcgaaa ccaatctcgc caccggcgac
    14101 agcagctcgc tgattctcgg cgaacctctg ccggtgcgtg atttcccgaa taccgacgcc
    14161 gacagcctgg cgctctattg gcaggacgag atcagcctcg gcgagcgctt tgcactgatc
    14221 ccgggcctgc gtgccgagtg gtatcgcctg cgcgcgaaac ccgacgcgat cttccgtgag
    14281 gattttccgg acgcgactcc ggtggatatc gacgaacagc agctcacgcc acgtctcgcg
    14341 ctgcgctggt cgccgggcgg cggtcacagc ctgttcgtac agtacgcgcg cggctttcgc
    14401 gcgccgcctt tcggcgatgt caacatcggt ctgatcctgc ccgtattcaa ttacgaagtg
    14461 cgcgccaatc cggacctgcg cccggaacgc agccaggggc tggaacttgg ctggcgccac
    14521 gtcggcgaca cgctgcgcgc cagcgtttcg ctgtacgaga accgctaccg ggacctgatc
    14581 gaatcgcgcg cgaatctcgg catcgaaccc gacaccggat tgctcgtatt ccagtcgatc
    14641 aaccgtgacc gcgcccgcat ccgcggtgtc gaagccgatc tgctctggaa cctgcccggg
    14701 cgcccggatg gcgccggcca ctggcagctg cgcggtgcgc tggcctgggc gcagggtgac
    14761 gatctgcgcc gcgggcagcc actcaacagc atcgacccgg cgcgcatcgc gctcggcctg
    14821 cgctacgagg cgttgtcgga tcggtggggc atggaagccg cgatggtcgg gacgcaggcg
    14881 aaagatcgca tcgaccacga gaacggcccg tactttgccc cgcccggtta cgtccgtttc
    14941 gatgcctacg cctgggccga accctggcgc ggcgtgcgca tcaacgtggg cgtcgtcaat
    15001 atcggcgacc gccgatactg ggactggtcc ggcgtgcgcg gcgtcggcgc gaacgatgcg
    15061 aacatcggct tctactcccg tcccggccgc agtctcgcag tgaaactggc catcgactgg
    15121 tgatgacggc gtacgaccag gaatatgtcg gcatgacttc ggccgacaca tgccggctga
    15181 cactcttcgg cgatttctcc gacggcagca ttgtttgtgc ggacggcgat aaattgctgc
    15241 gttgcctcaa gttgtccact agtatctgac cgacggccca gggcttcttc ggatggcccg
    15301 atgccccggc caccacactt gcggaccatg cctgcgacat agaggtagtc cgcggcaggg
    15361 ggtacggcta tgaaggcacg ccgaggcgga gccaagcggt ttcgcaggac ctttggggcg
    15421 ctggcggcaa tatgcctcgc cgccgccggc ctcgcccggg ccgatcagac cgtcgacgtc
    15481 accgtcatcc ccggcacgga gggcgatgac acgatcgccg tcctcacccc ggagcaggtc
    15541 gacgcgctga tcgcgagcat cattgccgag atcagcaagg cgagcgcaac gctgctcggc
    15601 tcgctcgcgg gcaacgacac cgtcacgatc gacgccgatc tagatgcctt ctccctggtc
    15661 ggtacgctga tcggttcgaa ccccggcgag tccgcaagca gcgcgaccag catcacggtc
    15721 gcgaccggca gcggcgtcga cttcgtggat gccgcggggg tgttcagttc gaccgcggac
    15781 tcgacgtccg cctacgccga cgtgttgccc gtggacgatg aattcctgcc gaccgggagc
    15841 cagaaggccg acgtctcgat caaggccact tcgcaagcca ccggtctcga taccggtgcg
    15901 gacaacgacg tggtcatcgc aagcgcggaa ctgtcgagtt ccgcggctgc gatcacgggc
    15961 ggcgttgcgg ccggcatcca ggcgtcgttc tcaaactccg tttccaccaa ggccggatcg
    16021 gttgccgaat cgaaggcggg cggcatccag gcggatgcgg gcaatgacca ggtcgcccac
    16081 cgcggcacga tcgtcgcgag tgccaccgcg gccagcggtg tgatcgcggt cgaggtcaat
    16141 gcttcggacg agaacgacgc cgagcgcaag gtgcagggca agctggacgc gagtgccaag
    16201 gccacggccg ggtccgtcgg catcgacacc gacggggcga gcacggagag cgatctggat
    16261 gtgacgctcg gcctgcccga cgacgcgatc caggtcaccg tggagtggtc ggagacgctc
    16321 gagagcggtg ccgatacggt cgcgaatctc ggcgcggtca cgagtaccgc gagcgcgatc
    16381 agcgacagcg tggcggtcgg cgtcagcgcc gcggccaagg gcagcctgaa ggcgaccatc
    16441 aatgccgagg cgaaggccgc aagtcgcgga atcaacagcg gcgcaggcga tgatgcggtg
    16501 acgaatgcgg gcagcatcgg cgcagccgcg gatgcagccg cctgggtcgt cggcgccacc
    16561 atctcggccg ggcagggggc ggacgagaac aaagccgcga agggcaaggc acacaccagc
    16621 accaacgcca ttgcagaatc ccgcgcgacc ggcatcgacg cggacggccc ggccgccagt
    16681 tcttcctcga cggtcgacac ggtgatcggc cgctccggta tttcgacgac ctacctgacg
    16741 agcgagacgg ccgcgaccgg tgtcgatgac gtcgtgaaca cgggtaccgt cacggtgaac
    16801 agctcggcgc tcgccgggtc ccgcgtcgtg accgtgcagg tcaacgccga aggcacggcg
    16861 cagtccgagg ccaattcgac cgcggactcc ggcggcatcg gcatcagtgc gggcggcggt
    16921 gacgatgcgg tggacaatcg cggcgtgctc accgtgggcg cggatgccac ggcgggcgcg
    16981 cttgcggtca ccttcgacca gggcggcgag aaagccgagt cgaaggtaga tgcgaaggtc
    17041 gaggctgccg cggtcgccac cggcatcgcg gccgatggcg gtgcggtgga tatcacgacc
    17101 gagatctcga tgacgatcga cgcggacggc ctcctcgccg agcgcaaccg gcagcagacg
    17161 acgcagcacg gcgacgacac gatactgaac agcgacgcgg tcggggcgac ggccacggca
    17221 ctcgcggggg ccgcgggagc cgcggtcgcg atagacggct ccgcgtcgtc cgacatgaca
    17281 tcgaaggccg gggcgacggc cgccggcatc gatggcggcg gcggcaacga cctcgtcgac
    17341 aacgaaggtg aactgaccgc gagtgcggat gcgagcgcgg gcgcgctgtc gatcggcttc
    17401 gcccagaagg tcgaggaaaa ggccgaggcc gaagtggagg tcaaggccac ggtggaggcg
    17461 gaggcaacgg ccacgggcct ggccggcgac agcggcaggg acgtggtcgc ggagtaccgc
    17521 ctggcgatcg gcgccgacgg gctcgacctc acgctgctga acaatgccgg ggcgctttcc
    17581 ggcgacgatc agctggtcaa ccgcggtgcc atcaccgcga ccgcaaattc gctcacgggt
    17641 gcagccggcg tctccgtgac gatcgatggc gcgtcggcgg ccgagctgga gtcgaaagcg
    17701 aaatcgcacg cctcgggcat cgacgccggc ggcggcaacg acctcgtgga aagcgccgga
    17761 tcggcgacgg ccgaggcgtc gtcgactgcc ggcgcgctgg gcgtggcctt tgcgcagaag
    17821 ccgggcccga aggagaacgc caaggccgag gtctcggccg aagccggcgc caccgcgacc
    17881 ggcatcgccg gcgacagtgg cgcgctcgtc gtcgaatcgc tcgaactcac gctcggcggc
    17941 gccggtctcg acagctcggc gagccgcgaa cgcaccgcca accgcggcga cgatgtgatc
    18001 ctcgcgaccg gcgacgtgac tgcgagcgca tatgcaacca gcgcgacgct cggcgtgacc
    18061 gtcgcgatcg acggcgcggc ggaggccgag atgaaggcca cggccgaagc gaaggccgca
    18121 ggcatccagg gtggcggcgg caacgaccgc gtcgagagca gcggactcgt caccacgctc
    18181 gcggacagct cggccggcgg cgtcgccgca gcggtcggcc agcagtcgag cgacggcagc
    18241 aaggccaaga ccaaggtttc ggcagagacc gaatccgtgg cgagcgcgac cgggatcgcg
    18301 ggcgatggcg gccaggagga cagcgtcgaa accgtcgcgg tcaatatagg cagctcgggc
    18361 ctgagcttcg gctacgagaa gacggccatc gcggcgagcg gcgacgatga actcttgaac
    18421 accggcgacg tggatacgac cgcggtcgcg gatacggccg cggcggcggt cgccgtaacc
    18481 gtcaagggcg gcgccaaggc ggacgtggac gcgaaggccg aagccacggc gcgcggcctc
    18541 gatgcgggcg gcggcgcaga caacatcacg aactcgggca cgctgacgac cgatgccacc
    18601 gcgacggcgc tcaccgccaa tgtttcggtc tccaccgaag gctcgtccat ggcgaacgcg
    18661 ggcttcctga gtggcgacaa cgaggtcgag gccgaggccg tcggcatctc gagcgcgggc
    18721 tcggaccacc agacgtcgac cgtcatcacg accgacatcg acttcacgga cttcgccgtc
    18781 acgggcagct acgagaaaat cgaagatcac gtctccggcg atggcgccga ccggatcttc
    18841 aactcgggcg aggtcacgtc cgacagcttc gcgacgacgc tcgcggcgga cgtcggcgtc
    18901 acgggcgaag gcagcgcgct ggcgctgggc cgggccaaag tcgaggcgca tgccggtggc
    18961 atcgagtccg gcaacggcga tgacacggtc gagaacaccg gcgcactgac gagcaacgcg
    19021 acggccacgg cggctctcgc gaacgtcgcc gtgacgggca agggtctcgc ggtcgcgggc
    19081 aattcggcct gggacggcgg cacgcatgcc gaggcgtcgg cggcaggcat cgatgccgac
    19141 agcggcgaga tcgaggccac gaccgtgacc ttcgaggcga gcctcgagcg tgcccaggtc
    19201 gtgtacgagg ggaccctcga ggccgcgacc ggcaacgacg tcgtgctgaa tgagggcgac
    19261 atcctggcgc aggcgatctc gactgcggcg acggcgtccg ttgccgtgag tccgaagggc
    19321 atgtcggcag cggtctcgac ctcgagcgcg gagtcggaaa ccttcggcat caagggcggc
    19381 ggcggcgacg accggatcga aaacacaggt ctcctcacct cctacgccga ctcgctggcg
    19441 gcgaccgcga gcgtgtcggt gacctcgggg cccggcgtcg ccgtggctgc ggatgcggtg
    19501 tgggactccg gcaccaaggc cgaggcgaag gcgatcggca tcgcgggcga cgggggtaac
    19561 cagagcgaga gcacgcggct cgcgatcggc accgacgaga tgggcctcga caaggacgag
    19621 gtcgtggcgg acggtgccga cacgatcctg aacttcggcg acatggacgt ggatgcaatc
    19681 gcgaccaccg catccgcgag cgtggccgtc acggcgagca gcggcgtcgc ggctgcgacc
    19741 gcaacctcga ccgccgaggc gacggccgcg ggcatcgacg ccggcgcggg cacgggcgtc
    19801 gaccaggtgt acaacgccgg catcctggac gtggcggcgg acgcggcggc cgcctcggcg
    19861 tcggtcagcg tggtcaacac gggcgttgcc atcgcggccg actcggtctg ggacggcggt
    19921 acgacggcca ccgcgcgcgc gcgcggcatc gacgtgggtg cgggcggcga cacgatcgag
    19981 aatgacggcg agacgactgc gaccgcaacc gcgctcgcgg gctccgcgac agtcgcggtc
    20041 acgagcaccg ggttcgccgg cgcctcgggg acctcgaccg gcaccgccga tgcctcggcg
    20101 ctcgacgcga gcgccggcat cgacagcgac accatcatca atcgcggtgc gctcaatgcc
    20161 gccgctgagg ccgccgccgc cacggcgaat gtcacgttca ccaatacggg cgcagcgctg
    20221 gcactcggtg ccgtctggga cgggggcacc ggcgcgaccg cgcgcgcgcg cggcatcgag
    20281 accggcgacg gcgcagacgg cgtcgtcagc accggcacga ttgccgccac cgccgacgca
    20341 ctggcggccg aggccgcggt gagcgtgacg gcgaccggag tcgccggcgc ggttgcgacg
    20401 gcgacggcgg agacggatgc cgtggcgatc gacctcggcg gcgacgcggt cgccgacacg
    20461 gtcgacaatc agggcgagct gacagtcgat tcgaatgcgc tggccttctc ggcggccgtt
    20521 tctgcgacca gcacgggcgt ggcggtctcc ggcggcgacg tctgggatgg cggtacgcag
    20581 gccacggccc gtgcgcgcgg catcgagacc ggtggcggcg cggacgggat cctgaacacg
    20641 ggcgccatcg gcctcgactc cgatgcggtg acggctgccg cgagcgtgag cgtggcgttg
    20701 accggcgttg gcggcgcgat ctcgcgctcg acctcggtct cggatgccac ggcgatcgac
    20761 gccggcgacg acgcgggcga cgatacggtg gagaactccg gcgatctgac cgtgaactcg
    20821 gatgcctttg cggcggcagt cagcgccgcc ttcacgagcg ccggtcatag gcgcccgcat
    20881 ttcctttctt ggaaggc
//
LOCUS       DAOQVY010000035        20865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_2986_length_20865_cov_6.014608, whole genome shotgun sequence.
ACCESSION   DAOQVY010000035 DAOQVY010000000
VERSION     DAOQVY010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 20865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20865
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2986_length_20865_cov_6.014608"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..3473)
                     /locus_tag="PKN91_05045"
     CDS             complement(243..3473)
                     /locus_tag="PKN91_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNS27475.1"
                     /translation="MNAGPRRLGVSGRLARAFLDSQLTPLLALTALLAGVFAVLVTPR
                     EEEPQIDVTMANVIVPFPGASAAQVEQLVSSPMEQILGEIDGVDHLYSVSQPGMAVLT
                     VQFEVGETRNEAVVRLYNAVYSNRDWRPAGLGVQEPLIRPKGIDDVPILTLALWTADE
                     DRAGYDLRHIAHSIEAELQRVPGTRDVYTIGGPDDAVLVRLDAQRLAGHGLGVDALMR
                     SLEAANIVAHAGKLVAGNRAIPVQAGEFLANRDDVANLVVGMHAGHPVYLQDVATVTR
                     GPAQPRSAVWFGGAGAPVKAPAVTIAVTKQPGSNAIDVADQLIERVDALRGVYLPTGV
                     ETTITRNYGETANDKAVKLIQKLLFATVAVIVLVILAVGRREAFVVGSAVVVTLAATL
                     FASWAWGFTLNRVSLFALIFSIGILVDDAIVVVENIHRHLQHAPPGEGGRDLPALIAH
                     AVDEVGGPTILATFTVIAALLPMAFVSGLMGPYMSPIPINASMGMVISLAVALVFTPW
                     LSRRLLQRIRAQDGGDTRASLAIDARLQRFFERLLGPFLHGADARRRRHRLYGATAAA
                     ILLAVSLAAFTLVVMKMLPFDNKSEFQVVVNMPEGTALERTTMLLDELAAELVKTPEV
                     THYQIYAGTSAPINFNGLVRQYDLRNAPHYGDIQVNLADQHERKRHSHEIALAVRPAL
                     AAIGRRHGAASVQVVEVPPGPPVLAPIVAEVYGPTPEARRALAQRLRGLFEATTDIVD
                     VDDTVEAPARREVIAIDRSRAALLGVSQEAAVRTLQVALDGYDATYVRAGQERYPIPV
                     RLELPVAGRATLDDLLALEVPAAGGTMVPLSEIVTVREAQWDGAIHHKDLLPVVHVTG
                     DMAGRLDSPLYGMFDLVGQLRAGDAGEEVAQYFIRQPADTLAAAVKWDGEWQITYETF
                     RDMGLAYSVGLLLIYLLVVAHFASYLVPLVIMAPIPLTIIGVMPGHALLGAQFTATSM
                     IGMIALAGIIVRNSILLVDFINQELAEGRAPAEAVIRAGAVRARPIVLTALAAMIGAV
                     FILDDPIFNGLAVSLIFGILVSTVLTLAVIPILYFSLLQARGAGQP"
     gene            complement(3470..4516)
                     /locus_tag="PKN91_05050"
     CDS             complement(3470..4516)
                     /locus_tag="PKN91_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNS27476.1"
                     /translation="MARRQLALTALVCASLCAPFAVTPAAEALAALTVETTMAPLERM
                     LDGRIEAVNQATVSAQTSGRIAEILYDVNDFVPAGAVILRLRATEQRAGLAQAQAALR
                     EAIALEAESQAHYTRTSNLYKEDAASKAQLEAALAARDAAVARLAAARAGLDAAREGI
                     SYTEVRAPYAGVVTQRLVEVGEAVRPGTPLMSGLSLQFLRVTVDLPQSVVEQVRGIRK
                     AAVYVDGQRIEATGLTIFPQASSAANTFRARVELPENAADLYPGMLVKVGFIVGEAQR
                     LTIPASALVERSEVTAVYVLGEGDRPSLRQVRVGRRSGERLEILAGLVDGERIAIDPH
                     AAARVLRESAGRAP"
     gene            complement(4529..4729)
                     /locus_tag="PKN91_05055"
     CDS             complement(4529..4729)
                     /locus_tag="PKN91_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNS27477.1"
                     /translation="MSIDRMVMRFAGLMVLAGLALGVYVHPNWLWLSAFVGANLFQAS
                     FTGFCPLAMVLKRLGVKSGAAF"
     gene            4820..5161
                     /locus_tag="PKN91_05060"
     CDS             4820..5161
                     /locus_tag="PKN91_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNS27478.1"
                     /translation="MAAHDTSPKRRAGSKMAADDMRAHAGDAAALLRPLANEQRLAIL
                     CCLLGGAQSVSQINAKVALSQSALSQHLAVLREAGIVETSREAQAVYYSLAPGPARRV
                     LAVMHDIFCAR"
     gene            5202..5636
                     /locus_tag="PKN91_05065"
     CDS             5202..5636
                     /locus_tag="PKN91_05065"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNS27479.1"
                     /translation="MDPASPEDDMLQQAIRQIATTPDPYAPFRISQAIEAGGFVFVSG
                     QAAMDLQGNLVGGNDFGAQAEQAFKMLGTVLEAAGSSLARVVKVTIYLTDMANFPAIV
                     QLREKYFTRPYPADTIVQVQRLALPELQIEIDAVALAGSAKG"
     gene            complement(5633..6415)
                     /locus_tag="PKN91_05070"
     CDS             complement(5633..6415)
                     /locus_tag="PKN91_05070"
                     /inference="COORDINATES: protein motif:HMM:NF025091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HNS27480.1"
                     /translation="MSTTTAAHRIAAARETFRALHARGCFVLANPWDIGGVRRAEKLG
                     YKAIASTSAGFAWSLGRNDNEASRDEVLAHLTALCHSTMLPVNADFEDGFAEQPEGVA
                     ENVRLAVATGVAGLSIEDRRGIDLYPIGPATERIRAARQAIDRSGENVLLVGRSEGLL
                     LGNTTIDTTVERLIAYAAAGADCVFAPGIRSITDIRSLVAAVAPTPVNVLLMDASMSV
                     AELAEAGVRRVSVGAAFAKAAWAAYEHAARSVIEAGHLPRGN"
     gene            complement(6549..7601)
                     /gene="ada"
                     /locus_tag="PKN91_05075"
     CDS             complement(6549..7601)
                     /gene="ada"
                     /locus_tag="PKN91_05075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HNS27481.1"
                     /translation="MKTSIVNDPRWAAITARDPAADGQFYYGVRTTGVYCRPSCPSRR
                     ARPENVELFDSRAAAEHAGFRACRRCRPDGHTLAGQQAAWVADACRIIDASEEPPTLA
                     ALAAQLKVSPYYFHRVFKSIVGVTPKQYAAARRGERLRRELARGKSVTAAIYDAGYNS
                     NARFYDQADDVLGMTASSFRAGGADTAIRFAVGECSFGSILVAATTRGICAILLGDDP
                     ETLTRNLQDQFPRAELIGGDVEFERLVARVVGFVEAPALGLDLPLDLRGTAFQQRVWQ
                     ALRAIPAGSTASYSEIARRIGAPKSVRAVGQACGANNLAVAIPCHRVVRSDGDLSGYR
                     WGIERKRALLAREARE"
     gene            7728..8156
                     /locus_tag="PKN91_05080"
     CDS             7728..8156
                     /locus_tag="PKN91_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27482.1"
                     /translation="MSDFQPRWLRKGRRVVPPAALRATLQELFGEGVDTVQVIEHSWF
                     AWAHVRAIATTRRRRIYLRGAAGPFFANPEFVLHEYFHVLAQWEPRQLSVWKYLLECF
                     RRGYWDNRFEIEAREFSEDHRRRYCALLARHESLRSHRHA"
     gene            8149..9573
                     /gene="dacB"
                     /locus_tag="PKN91_05085"
     CDS             8149..9573
                     /gene="dacB"
                     /locus_tag="PKN91_05085"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNS27483.1"
                     /translation="MRSGSRRSKPGIAAALSAALLATLATAQAATLPEVYANALRAAG
                     IPVGAVGVVVMPLDGQGLEAEANRNRPMNPASTMKLLTTYAALRTMGPAYAWRTDVAM
                     LGAREGDVILGDLAIRGGGDPSLVVEQLWLLVQKVRGLGIREIRGDLVLDRSAFQLPP
                     HDMAAFDGDALRSYNVGPDALLVNHKSISYSFVPDEPAGVVRLAIIPPLAGSKAPATV
                     PAANGACGDWRGKLRADFSRPFAPVFRGAFPLACGERSWNLSPIEPNAYFAAVFRALW
                     EASGGRWVGKAREGIVPQGSVLLATHVSKPLAEVVRDVNKYSNNVMAQQVYLAFGAER
                     IGWPASFERSGVAMRAWLAEAGLAMPELVLENGAGLSRSERISADSLARLLADAYRSP
                     VMAELMASMPVVGVDGTAALRRGAAGAAHVKTGLLNEVRAVAGYVLALSGRRYVVVSI
                     VNHANAGGTQRAHDALLEWVHREG"
     gene            complement(9578..10807)
                     /gene="gltS"
                     /locus_tag="PKN91_05090"
     CDS             complement(9578..10807)
                     /gene="gltS"
                     /locus_tag="PKN91_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HNS27484.1"
                     /translation="MKLDLIQTVAFAGVALFLGYGLRRLFPLLARLNMPAPVLGGLIV
                     SVAILIVRGQDLPVPEFDATLQRPLMVAFFTSIGFAASLKLLRIGGPQVAVFLAIGTV
                     FAVLQNLLGAGVAVLFDRPPLFGVLAGSVTLTGGPATGLAFAPLFEAAGIEGAAAIAI
                     AVAMAGIVLGGLVGGPIATHLIERYRVPGPRQRAKGEGVTADAQAAQADEPAAPSSDD
                     LDDESVEAYTALKTVVVVLVAMWVGEWVSALIGATGLTLPSYIGAMIAAAAIRGIDDY
                     TGLIGLSHRTIDTLGAITLSLFLVMALMTLDLTQLAGLALPLVTILAAQIVLVAIACL
                     PVFWLMGRDYDAAVMGGGFVGFMLGTTANAMAVMRTLVERFGPAPRAFLVAPLVGTFF
                     IDFTNALIITVFLNLFA"
     gene            complement(10832..12010)
                     /locus_tag="PKN91_05095"
     CDS             complement(10832..12010)
                     /locus_tag="PKN91_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNS27485.1"
                     /translation="MGAVGIVWRKEVRENLRDRRTLVSALLFGPLFGPLILAAALQFL
                     VQRTADEADQRLEIAVRHVERAPNLVAWLEARNVIVTPVELDDEATRRAVRERSHPLV
                     LSIPADFAERLAAARPAPLQLYSDAAGGRADRNAARTRMLIAQYGASIGRLRLLARGV
                     DPLLTAPLAVQDIDVSTPQSRSVTVLGMLSYLLLLAMILGGLYLAIDATAGERERHSL
                     EPLLTTPVPRARLIYGKILAATTFMIVSLTLTAIACALALRFVALENFGMSAHLDASV
                     VLKIILVTAPLAPAAAGLMTVVASFTRSYREAQTWLGVVMLVPTLPIAFVGLAELQPS
                     LALSAVPSLGQHFLITSLLRGAALEPAHVLVSVLASLALGAVLAWIAGRLYRRESLLG
                     "
     gene            complement(12010..12741)
                     /locus_tag="PKN91_05100"
     CDS             complement(12010..12741)
                     /locus_tag="PKN91_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS27486.1"
                     /translation="MMSAQGLAKAFGGVQAVRGVTLRAEDGAITGLLGPNGAGKSTTL
                     RMLCTVLRPDAGEAWIDAAHTVRDALDARRQLGMLTHDSGLYPHLTARENILYFAELH
                     GMARAARERRADELIEWLEMGAFAGRRAKGYSQGQRIKTALARALVHSPRNVLLDEPT
                     NGLDVMAVRNLRVLLARLRDAGHCVLFSSHVMQEVSGLCDVVFVLARGEVVAHGTPDA
                     LRAATGCASLEDAFVKLIGTDEGLG"
     gene            complement(12752..14170)
                     /locus_tag="PKN91_05105"
     CDS             complement(12752..14170)
                     /locus_tag="PKN91_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNS27487.1"
                     /translation="MLTALALAVAWAPCQLTHPAGVATVAAECTTLQVPERAADPDGR
                     RIGLFVARVPAVNARKTPDPLFVLAGGPGAAASEFYAGVAPAFARVNRDRDIVLIDQR
                     GTGRSNALHCDFDDEALLGADDARFLAQTRRCLEEVSTRADVREYTTSAAVRDLEFAR
                     RALGYERINLYAVSYGTRVAQHYLRHHGARVRTVTLDGTVPAERILGPDIAVDAEAAL
                     AQVLARCANDAACRGAFGDPVVHYRALRSRLEEQPAGMLLPAPRTGERVAVEFGPAHL
                     ATVLRLSTYGAEQAALLPLALYEAHMHRNFVPLASQFLLMSDSLSGQMAYGMHHSVVC
                     TEDAPWFPDTRADRVVLAATFIGTRQLDGLAALCEIWPRGVIDDDLHAPLASEVPALL
                     LSGSADPVTPPAGAAHAAAGFRHSRHVIVEGFGHGQIGAPCVDRLLAAFVTSGTAEGL
                     DAACVERLRPMPFFTTLAGPAP"
     gene            14221..16053
                     /gene="mnmG"
                     /locus_tag="PKN91_05110"
     CDS             14221..16053
                     /gene="mnmG"
                     /locus_tag="PKN91_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNS27488.1"
                     /translation="MDFFDVIVIGGGHAGTEAALAAARMGRRTLLLTQSLETLGAMSC
                     NPAIGGIGKGHLVREIDALGGAMARAIDRAGLQFRTLNASKGPAVRATRAQADRVLYR
                     REIRALLENQPNLSLFQQEAADLVVEGERVCGVVTVTGIVLRARAVVLTVGTFLGGRI
                     HVGLDNYQGGRAGDPPSNRLAARLRELPLRVGRLKTGTPPRIDGRTIDFTRLTEQWSD
                     APAPVFSFLGRASEHPAQRPCHITATNTQTHDIIRAATDRSPMFTGVIEGVGPRYCPS
                     VEDKVVRFADKASHQVFLEPEGLDTHEVYPNGISTSLPYDVQLRFVHTIKGLERAHLT
                     RPGYAIEYDYFDPRDLAPSLATKALAGLYFAGQINGTTGYEEAAAQGIVAGINAARAA
                     REEAPWVPQRAEAYLGVLIDDLVTRGVTEPYRMFTSRAEHRLLLREDNADLRLTPVAR
                     ELGLVDDERWAFFEAKRRATDAEVARLERTRVRAVSAMELLRRPEVSYEGLLAQIGLP
                     AADAYDDERIAREVALQVTVRAKYAGYIERQHAEVERQRAHETAALPADLDYLAIGGL
                     SNEARQRLAEVRPATLGQAARVPGITPAAISILMIHLKRRGRAA"
     gene            16043..17638
                     /locus_tag="PKN91_05115"
     CDS             16043..17638
                     /locus_tag="PKN91_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNS27489.1"
                     /translation="MRRSLALVLGAFALAALGGCAPPPPETSNILLRGNGPEPDSLDP
                     QKARSVEALTVLRDLYECLTSLGRDAETVPGVAETWAASDDGLTYTFRLRDNARWSNG
                     DSVTGEDFVFALRRLVDPATASQYAQLVDVIDNAAEIVAGRAAPEALGVSAPDPHTVV
                     VRLASPAPYLPGLLSFPSTCPVHRPTLEKEGARFTRPGVMVSNGAFRLTEWVPGSHIS
                     VVRNRAYWNDAATRLEGVKYLQIADESAEFTRYRAGDLHTTAVVPRGQFDVIRKDHAA
                     ELHIGPQLSTYYYGFALDRPPFRGAPGLRRALSLVIDRERLAESVLRVGELPAYGWVP
                     PGVHQYTSQTFGYAGLPMAERIEEARRLYAAAGYSAARPLRFELRYNSGEVHSRLAVA
                     VASMWKEALGVEAQLTAVEFRSLMADVDARNVEMFRLSWSGDYNDAYTFLQYLKSDFG
                     INLPRYANPAYDALLARAAGASDATERRNLLETAERMMLADTPLLPLYFYVNKHLVSP
                     RVEGWYDNVMNVVYSKDLALAAP"
     gene            complement(17767..18249)
                     /locus_tag="PKN91_05120"
     CDS             complement(17767..18249)
                     /locus_tag="PKN91_05120"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNS27490.1"
                     /translation="MAEHKATIDWAREGATFTDNRYSRAHRWRFDGGVEVPGSASPHV
                     VPLPHSVEAAVDPEEAFIASLSSCHMLWFLSIAAERGFVVDRYVDEAAGRLGRDAAGR
                     MAMTRVILHPAVQFSGGKLPTDDEIAGMHDEAHDKCFIANSVKTEVRCEPRPWVPSSP
                     "
     gene            complement(18268..20019)
                     /locus_tag="PKN91_05125"
     CDS             complement(18268..20019)
                     /locus_tag="PKN91_05125"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HNS27491.1"
                     /translation="MRKLLWLLAASLIAAPGTSLATDARLQALFDAAWEADLRDNPTA
                     ADYLGDHRYSDQWPDLSAAALEARYARYAAVMAELKAIPRDGLSGADRLNYDLFAQEY
                     ERRLAAQPFKPWLYEAIRPRDGVQSASELAELLPFATVADYEAWIVRLTKFGTYIDQH
                     IAMLATAIREGRTQPRSIMQRIPAQLANQIPANAEDSPFFAPFTRMPEDIGEEDRARL
                     IGAGGRAIVEVVIPAFKRLDAFFRDEYLPAARSSDGIWDTPDGLAFYANRTAFHTTTD
                     LTPDDIHEIGLREVARIRAEMDEVIARVRFQGTFEKFLAFLRTDPQFYYGSGEELFTA
                     YAAMAKRIDPELVKLFGKLPRTPYGVREIPMTSAPDTTTAYYQGPAIDGSRAGYYYVN
                     LYRPEVRPKYEIPVLTVHEAVPGHHLQIALAMEDRTLPLFRRNADFTAYVEGWALYSE
                     RLGEQMGLYDDPYDKFGQLTYDMWRAVRLVVDTGIHARQWTRQQAIDYFKANAAKTEA
                     DIVNEVDRYIAWPGQALAYKIGQLRILDLRREAQAALGGRFDIRAFHDTVLASGPVPM
                     DVLTEQVHAWINSQKQQ"
     gene            complement(20057..20530)
                     /locus_tag="PKN91_05130"
     CDS             complement(20057..20530)
                     /locus_tag="PKN91_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27492.1"
                     /translation="MRGVLHPDPRRVEVPPDHFDVASDDVLFSALLDGTLAVCLYDAV
                     EEHGALLHLRFIARGANQGDVTDRTLASDLLLLDRCVEGFRDAMERGVDVQGKIAASV
                     ESGEPARQAAAAVLALIGEYLIDAGVRIVSHEVEYGVARRLRFRPSMGQLRLDPA"
     gene            20642..>20865
                     /locus_tag="PKN91_05135"
     CDS             20642..>20865
                     /locus_tag="PKN91_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNS27493.1"
                     /translation="MLDIGFLPIIILVAIIIVASKAWLTVPQGYEYTLERFGKFKKSM
                     EPGFHFIVPFVEAIGRKINMMEQVLDVPRQ"
BASE COUNT         3267 a         7165 c         7326 g         3107 t
ORIGIN      
        1 ggtgccgtga cgccggctgt cgctgcgggc ctccgtccgc cacggcgcag acaggtcctg
       61 cgtacgggcc atggcgtcga accaggtgca ccagccccgc gatatccctg cgaggagcgg
      121 tgacgaccag atgttgatgt acattgttct ctccttgtcc gaatggacag tttgtggaga
      181 ggatcatggc cgacgggtgg gccctttgag ttccgaaatt ctcgcgctcg cgtaagttgc
      241 gcttaaggct ggccggcgcc cctggcctgc aacaggctga agtacaggat cggaatcacc
      301 gcgagcgtga gcacggtcga gacgaggatg ccgaagatga gcgacaccgc gaggccgttg
      361 aagatcggat cgtcgaggat gaacaccgcg ccgatcatcg ccgcgagcgc ggtcagcacg
      421 atcgggcggg cgcggacggc accggcgcgg atcacagcct cggccggtgc gcggccctcg
      481 gccagctcct ggttgatgaa gtcgaccagc aggatcgagt tgcgcacgat gatgccggcg
      541 agcgcgatca tgccgatcat cgaggtggcc gtgaactgcg cgccgagcag tgcgtgaccc
      601 ggcatcacgc cgatgatcgt cagcgggatc ggcgccatga tcacgagcgg cacgaggtag
      661 gaagcgaagt gcgcgacgac cagcaggtag atcagcagca ggcccaccga gtaggcgagc
      721 cccatgtcgc ggaaagtttc ataagtgatc tgccactcgc cgtcccactt cacggcggcg
      781 gcgagcgtat cggcgggctg gcgaatgaag tattgcgcca cttcctcgcc ggcgtcgccg
      841 gcgcgcagct ggccgacgag gtcgaacatg ccgtagagcg gactgtcgag ccgcccggcc
      901 atgtcgccgg tgacatgaac gaccggcagc aggtccttgt ggtggatcgc gccatcccac
      961 tgcgcctcgc gcacggtgac gatctccgat agcggcacca tcgtgccccc ggccgcgggc
     1021 acctcgagcg cgagcaggtc gtcgagcgtt gcacgcccgg cgaccggcag ttcgaggcgc
     1081 accgggatcg gatagcgctc ctgtccggcg cgcacatagg tcgcgtcata gccgtcgagt
     1141 gcgacctgca gcgtgcgcac cgccgcctcc tggctcacgc cgagcagggc cgcgcgcgag
     1201 cggtcgatcg cgatgacctc gcgccgcgcc ggtgcctcga ccgtgtcgtc gacatcgacg
     1261 atatccgtcg tcgcctcgaa caggccgcgc agccgctgcg caagggcgcg ccgtgcctcg
     1321 ggcgtcgggc cgtacacctc ggcgacgatg ggcgcgagta ccggcggacc gggcgggact
     1381 tcgacgacct gcacgctcgc cgcgccgtgg cggcggccga tcgcagcgag cgccggccgc
     1441 acggcgagcg cgatctcgtg gctgtggcgc ttgcgttcgt gctggtcagc gaggttgacc
     1501 tggatgtcgc cgtagtgggg cgcattgcgc aggtcgtact ggcgcacgag tccgttgaag
     1561 ttgatcggtg ccgaggtgcc ggcgtagatc tggtagtgcg tgacctccgg cgtcttgacg
     1621 agttccgcgg cgagctcgtc gagcagcatc gtggtgcgtt cgagtgcggt gccctcgggc
     1681 atgttcacga cgacctggaa ctccgacttg ttgtcgaagg gcagcatctt catcacgacg
     1741 agcgtgaacg ccgcgagcga taccgcgagc aggatcgccg ccgcagtcgc accgtagagg
     1801 cggtggcgcc gacgacgggc gtcggcgccg tggaggaagg ggcccagcag gcgttcgaaa
     1861 aagcgctgca gtcgcgcgtc gatggcgagc gacgcgcgcg tatcgccacc gtcctgcgcc
     1921 cggatccgct gcagcagccg tcgtgacagc cacggggtga agacgagcgc gaccgcaagc
     1981 gagatcacca tgcccatgct cgcgttgatc gggatcgggc tcatgtaggg gcccatcagg
     2041 ccactgacga aggccatcgg cagcagcgcg gcgatcaccg tgaaggtcgc gaggatcgtc
     2101 ggcccgccga cttcgtcgac cgcatgcgcg atcagcgcgg gcaggtcacg gccgccctcg
     2161 ccgggaggcg cgtgctgcag gtgacgatgg atgttctcca ccaccacgat cgcatcgtcg
     2221 accaggatgc cgatcgagaa gatcagcgcg aacagcgaga cgcggttcag cgtgaagccc
     2281 caggcccagg aagcgaacag ggtcgcggcg agcgtgacga ccacggcgct gccgacgacg
     2341 aaggcttcgc gccggccgac ggcgaggatg accagcacga tcaccgcgac ggtcgcgaac
     2401 agcagcttct gtatcagctt gaccgccttg tcattcgcgg tctcgccgta gttgcgggtg
     2461 atcgtcgtct cgacgcccgt cggcaggtac acaccgcgca gtgcatcgac ccgctcgatc
     2521 agctggtcgg cgacgtcgat cgcattgctg ccgggctgct tggtgaccgc gatcgtcacg
     2581 gccggggcct tgaccggcgc gccggcgccg ccgaaccaga ccgccgagcg cggctgtgcg
     2641 ggcccgcgcg tcaccgtcgc gacatcctgc agatagaccg ggtggcccgc gtgcattccg
     2701 acgaccaggt tcgcgacatc gtcgcgattg gcgaggaact ctccggcctg caccggaatc
     2761 gcacggttgc ctgcgacgag cttgcccgcg tgcgcgacga tgttggccgc ctccagcgag
     2821 cgcatcagcg catcgacgcc gaggccgtgg ccggcgagcc gctgggcgtc gaggcgcacg
     2881 agcaccgcgt cgtcgggccc gccgatcgtg tagacgtcgc gcgtgcccgg cacgcgctgc
     2941 agctcggctt cgatcgagtg cgcgatgtgg cgcaggtcgt agcccgcgcg gtcctcgtcg
     3001 gcggtccaca gcgcgagcgt caggatcggc acatcgtcga tgcccttcgg gcggatcagt
     3061 ggctcctgca cgccgagccc ggcggggcgc cagtcgcggt tggaatagac cgcgttgtag
     3121 aggcgcacga ccgcttcgtt ccgagtctca ccgacttcga actgcacggt cagcaccgcc
     3181 atgcccggct gcgagaccga atagagatgg tcgacgccgt cgatttcgcc gagtatctgc
     3241 tccatcggcg aactgacgag ctgttcgacc tgcgcggcgc tcgcgccggg gaacggcacg
     3301 atcacgttcg ccatcgtcac gtcgatctgc ggctcctcct cgcgcggggt gaccagcacc
     3361 gcgaacacgc ctgcaagcag cgcggtcagc gcgagcagcg gagtaagctg gctgtcgaga
     3421 aaggcgcgcg caagacgccc gctcacgccg aggcggcgcg ggccggcgtt catggcgcgc
     3481 ggccggccga ttcgcgcagc acgcgcgcag cggcgtgcgg gtcgatggcg atgcgttcgc
     3541 cgtcgacgag cccggcgagg atctcgagcc gctcaccgct gcggcggccg acgcgcacct
     3601 gtcgcaaact cgggcgatcg ccctcgccga gcacgtatac ggcggtcact tcactgcgct
     3661 cgaccagcgc actggccgga atcgtcagcc gctgcgcctc gccgacgatg aagccgacct
     3721 tgacgagcat cccggggtag agatcggcgg cgttctcggg cagctcgacg cgcgcgcgaa
     3781 aggtgttcgc ggcggagctc gcctgcggaa agatcgtgag accggtggcc tcgatgcgct
     3841 ggccgtcgac gtaaacggcg gccttgcgga tgccgcgcac ctgctcgacg acgctttgcg
     3901 gcaggtcgac cgtgacgcgc aggaactgca gcgacaggcc gctcatcaac ggcgtgccgg
     3961 gccgcacggc ctcgccgact tcgaccaggc gttgagtaac gacgcctgcg tacggcgcgc
     4021 gaacctcggt gtagctgatg ccctcgcgcg ccgcatcgag acctgcgcgg gccgcagcga
     4081 gccgggctac cgcggcgtca cgcgcggcaa gggccgcctc gagctgggcc ttcgatgccg
     4141 cgtcttcctt gtacaggttg ctggtgcgcg tgtagtgcgc ctgcgattcg gcctcgaggg
     4201 caatcgcctc gcgcaatgcc gcctgcgcct gcgcgaggcc ggcacgctgc tcggtcgcgc
     4261 gcaggcgcag tatgaccgcg cccgccggca cgaagtcatt gacgtcgtag aggatctcgg
     4321 cgatgcgccc cgaggtctgc gccgacacgg tggcctggtt cactgcctcg atcctgccgt
     4381 cgagcatgcg ctcgaggggc gccatggtcg tctcgacggt cagcgcggcg agtgcctcgg
     4441 cggccggcgt caccgcgaac ggtgcgcaga gcgatgcgca gacgagcgcg gtgagcgcga
     4501 gttgtcgcct tgccacgatg cgtcgccttc agaacgccgc gccggacttg accccgagcc
     4561 gcttcagcac cattgcgagc gggcagaagc ccgtgaagga tgcctggaac aggttggcac
     4621 cgacgaatgc gctgagccac agccagttcg ggtgcacata gacaccgagc gcgagaccgg
     4681 cgagcaccat caggcccgcg aacctcatca ccatgcgatc gatactcata gctcgctcct
     4741 cccgggcccg gaagaggccc gctccaagcc agtatattag tattcgctaa tttagcaata
     4801 catgatatag aatgcgcgaa tggcagctca tgatacctcc ccgaagcggc gcgccggctc
     4861 gaagatggcg gcagacgaca tgcgtgccca tgccggcgac gcggcggccc tcctgcgtcc
     4921 gctcgcgaac gaacagcggc tcgcgatcct ctgctgtctg ctgggcggcg cgcagtcggt
     4981 cagccagatc aatgcaaagg tggctttgag ccagtcggcg ctgtcgcagc acctcgccgt
     5041 gctgcgcgag gccggcatcg tcgagacgag ccgcgaggcg caggccgtgt actactcgct
     5101 cgcgcccggt ccggcgcgtc gggtgctcgc ggtgatgcac gacatcttct gcgcccggtg
     5161 accggggtga ggcgccctgc ggccggcagc ggatcggcat aatggatcca gccagtccgg
     5221 aggacgacat gctgcagcaa gccattcgcc agatcgccac cacgcccgat ccctacgctc
     5281 cgttcaggat ttcgcaggcc atcgaagccg ggggcttcgt cttcgtgtcc ggccaggccg
     5341 cgatggatct gcagggtaac ctcgtcggcg gcaacgactt cggcgcgcag gccgaacagg
     5401 cgttcaagat gctggggaca gtgctcgagg cggcgggttc gagcctcgcg cgcgtggtca
     5461 aggtcacgat ctacctgacc gacatggcga atttcccggc gatcgtccag ctgcgcgaga
     5521 aatatttcac gcggccgtat cccgccgaca cgatcgtgca ggtccagcgc ctcgcgctcc
     5581 cggaactgca gatcgagatc gatgcggtgg cgctcgcggg cagcgcgaag ggctagttgc
     5641 cgcgtggcag gtgtcccgcc tcgatgacgc tgcgtgcggc gtgctcgtag gccgcccagg
     5701 ccgccttcgc gaatgcggcc ccgacgctga ccctccgcac gcccgcctcg gcgagttcgg
     5761 ccacgctcat cgatgcatcc atcaacagca cgttgaccgg agtcggggcc acggccgcca
     5821 ccagtgacct gatgtcggtg atggagcgga tcccgggcgc gaacacgcag tcggcgcccg
     5881 cggcggcgta ggcgatcaat cgctcgacgg tcgtgtcgat ggtggtattg ccgagcaaca
     5941 gcccctcgct gcggccgacc agcagcacgt tctcgccgct gcggtcgatc gcctggcgcg
     6001 ccgcgcggat gcgctcggtt gcaggtccga taggatagag atcgatgccg cgacgatcct
     6061 cgatggacag tcccgcgacg ccggtggcga ctgcgaggcg cacgttctcg gccacgccct
     6121 cgggttgttc ggcgaatccg tcctcgaaat cggcgttgac cggcagcatg gtcgagtggc
     6181 agagcgcggt gagatgcgcg agcacttcgt cgcgcgaggc ttcgttgtcg ttgcggccga
     6241 gcgaccaggc gaagccggcg ctcgtcgagg cgatcgcctt gtagccgagc ttctccgccc
     6301 ggcgcacgcc gccgatgtcc caagggttcg cgagcacgaa gcagccacgc gcgtgcagtg
     6361 cgcgaaacgt ttcgcgggcc gcggcgatcc tgtgggcggc ggtggtggtg ctcatggcga
     6421 tgctcctcgg gccgcaggct cacgcggcat cctggaaggt tacgcccaac gcgtgtcgat
     6481 cgtccgtcgg cctgcgaccg gtcgtgcggg cgctcggccg ggatgaccgc gcccgccccg
     6541 gttcggcctc actcccgcgc ctcgcgcgcg agcaaggcgc gcttgcgctc gatgccccag
     6601 cgatagccgg agaggtcgcc gtcgctgcgc accacgcggt ggcaggggat tgccaccgcg
     6661 aggttgttcg cgccgcaggc ctggccgacg gcgcgcaccg acttcggtgc gccgatgcgt
     6721 cgcgcgattt cgctgtagct ggcggtcgag cccgccggga tcgcacgcag cgcctgccag
     6781 acgcgctgct ggaacgccgt tccgcgcagg tcgagcggca ggtcgagccc gagcgccggt
     6841 gcttcgacga aacccacgac cctggcgacc agtcgctcga actccacatc gccgccgatc
     6901 agttcggccc gcgggaactg gtcctgcagg ttgcgcgtga gcgtctccgg gtcgtcgcca
     6961 aggagaatcg cgcagatgcc gcgtgtagtc gcggcgacca ggatagagcc gaacgagcac
     7021 tcgccgaccg cgaaccgtat agcggtatcg gccccgccgg cgcggaagct ggatgcggtc
     7081 atgccgagca cgtcgtcggc ttgatcatag aagcgggcgt tggagttgta gcccgcgtcg
     7141 tagatcgccg ccgtcaccga cttgccgcgc gcgagttcgc gccgcaggcg ctcgccgcgc
     7201 cgcgcggcgg catactgctt cggcgtcaca cccacgatcg atttgaatac gcgatggaag
     7261 tagtacgggc tcaccttcag ctgcgcggcg agcgccgcga gggtcggggg ctcctcgctc
     7321 gcatcgatga tgcggcaggc atcggccacc caggcggcct gctgtccggc gagggtgtgt
     7381 ccatccggcc gacagcgcct gcaggcgcga aagcccgcgt gctcggccgc tgcgcgcgaa
     7441 tcgaacagct cgacgttctc gggccgcgcg cgccgcgacg ggcacgacgg ccggcaatac
     7501 acgccggtcg tgcgcacgcc gtagtagaac tggccgtccg cggctgggtc tctagcagtg
     7561 atcgccgccc agcgaggatc gttcacaatg gaagttttca tggctgcgag tctgcgggtc
     7621 cccggagctg ccggcacccc ggtccttgct ttcgtattca agcacagccg ggagttgccg
     7681 ggcactccgc gcacgttagc gtcttttagc cacatgaacc agtcaatatg agtgattttc
     7741 agcccagatg gctgcgcaaa gggcgccggg tcgtgccacc ggcggccctg cgggccacgc
     7801 tgcaggagct gttcggcgag ggggtcgaca cggtgcaggt gatcgagcac tcgtggttcg
     7861 cctgggcgca cgtgcgcgcc atcgccacca cccgccgccg ccgcatctac ctgcgcggcg
     7921 cggccggccc gttcttcgcc aatccggaat tcgtgctgca cgaatacttc cacgtgctcg
     7981 cccagtggga gccgcggcag ctctcggtgt ggaaatatct gctcgagtgc ttccgccgcg
     8041 gctattggga caaccgtttt gaaatcgaag cccgtgagtt ttccgaagat catcgccgcc
     8101 gctactgcgc gctgctcgcg cgccacgaat ccttgcgctc gcaccggcat gcgtagcgga
     8161 tcgcggcgct cgaagcctgg catcgccgct gcgctgtccg ctgcgctgct cgccacgctt
     8221 gcgaccgcgc aggccgcgac tttgcccgag gtctacgcga atgcgctgcg cgcggcgggt
     8281 attccggtgg gcgccgtcgg cgtcgtcgtc atgccgctcg atggacaggg tctcgaagcc
     8341 gaggcgaatc gcaaccggcc gatgaacccc gcctcgacga tgaagctgct gacgacctac
     8401 gcggcgctgc gcacgatggg tcccgcctat gcctggcgca ccgacgtcgc catgctcggt
     8461 gcgcgcgagg gcgatgtcat cctcggtgac ctcgcgatcc gcggcggcgg cgatccgtca
     8521 ctggtcgtcg agcaactctg gctgctcgtg cagaaggttc gcggcctcgg catccgcgag
     8581 atccgcggcg acctggtgct cgaccgcagc gccttccagc tcccgcccca cgacatggct
     8641 gcattcgacg gcgacgcgct gcgcagctac aacgtgggtc ccgatgcgct gctcgtgaat
     8701 cacaagtcga tcagctacag cttcgtgccc gatgagccgg ccggggtcgt gcgcctcgcg
     8761 atcataccgc cgctcgcggg atcgaaggcg ccggccacgg tacccgccgc gaacggtgcc
     8821 tgcggcgact ggcggggcaa gctgcgcgcc gacttctcgc gccccttcgc gccggtgttt
     8881 cgcggcgcct ttccgctcgc ctgcggcgag cgcagctgga acctgagtcc catcgagccg
     8941 aacgcgtatt tcgccgccgt gttccgcgcg ctgtgggaag cgagcggcgg ccgctgggtc
     9001 ggcaaggcgc gcgagggcat cgtgccgcaa ggcagcgtgc tgctcgccac gcacgtctcg
     9061 aagccgctcg ccgaggtggt gcgcgacgtc aacaagtaca gcaacaacgt gatggcgcag
     9121 caggtgtatc tcgccttcgg cgcggagcgc atcggctggc cggcgagctt cgagcgctcc
     9181 ggggtcgcga tgcgcgcgtg gctcgcggag gccggcctcg ccatgcccga gctggtgctc
     9241 gagaacggcg cgggtctctc gcgcagcgag cgcatcagtg ccgattcact cgcgcgcctg
     9301 ctggcggatg cgtatcgcag cccggtgatg gccgagctga tggcgtcgat gcccgtggtc
     9361 ggtgtcgacg gtaccgccgc gctgcgcagg ggcgcggccg gcgcggcgca cgtcaagacc
     9421 gggctgctca acgaagtgcg cgcggtggcg ggctatgtgc tcgcgttgag cgggcgccgc
     9481 tacgtcgtgg tctcgatcgt caaccacgcc aatgccggcg gcacgcagcg cgcgcacgac
     9541 gcgctgctcg agtgggtgca ccgcgagggc tgaagcgcta cgcgaacaag ttcaggaaca
     9601 ccgtgatgat cagcgcgttg gtgaagtcga tgaagaaggt gccgacgagc ggcgcgacca
     9661 ggaacgcacg cggcgccggc ccgaagcgct cgaccagcgt gcgcatcacc gccatcgcat
     9721 tggcggtcgt gccgagcatg aagccgacga agccgccgcc catcaccgcg gcgtcgtagt
     9781 cgcgccccat cagccagaac accggcaggc aggcgatcgc caccagcacg atctgcgcgg
     9841 cgaggatcgt cacgagcggc agcgcgagtc cggcgagctg ggtcagatcg agcgtcatca
     9901 gcgccatcac gaggaacaac gacagcgtga tcgcgccgag cgtgtcgatc gtgcgatgcg
     9961 agaggccgat cagcccggtg taatcgtcga tgccgcggat cgcggccgcg gcgatcatcg
    10021 cgccgatgta ggagggcagc gtgaggcccg tcgcgccgat cagcgcgctc acccactcgc
    10081 cgacccacat cgcgaccagc acgacgacca ccgtcttgag tgccgtgtag gcctcgacgg
    10141 attcatcgtc gagatcgtcg gacgagggtg cggccggctc gtcggcctgc gcggcctgcg
    10201 cgtccgcggt gacgccctcg cccttcgcgc gctggcgcgg cccgggcacg cgatagcgct
    10261 cgatcaggtg ggtggcgatc ggtccgccga cgagcccgcc cagcacgatg cccgccatcg
    10321 cgaccgcgat cgcgatcgcg gcggcgccct cgatgcccgc cgcctcgaac agcggcgcga
    10381 aagcgaggcc ggtcgcggga ccccccgtca gcgtgaccga gccggccagc acgccgaaca
    10441 gcggtgggcg gtcgaacagc accgcgacac cggcgccgag caggttctgc agcaccgcga
    10501 acacggtgcc gatcgcgagg aacaccgcca cctgcgggcc gccgatgcgc agcagcttca
    10561 gactcgccgc aaaaccgatc gaagtgaaga acgcgaccat cagcgggcgt tgcagcgtcg
    10621 cgtcgaactc cggtaccggc aggtcctgcc cgcgcacgat caggatcgcc accgagacga
    10681 tcaggccgcc gagcaccggg gcgggcatgt tcaggcgcgc gagcagcggg aacagccgcc
    10741 gcagtccgta gccgaggaag agggcaacgc cggcgaaggc aacggtctgg atcagatcga
    10801 gcttcatgga cgaaggatag cggttcgggc ctcagccgag cagggattcg cggcgataga
    10861 gtcggccggc gatccacgcg agcaccgcgc cgagcgcgag gctcgccagc acggacacga
    10921 gcacgtgggc cggctcgagc gccgcgccgc gcagcagcga ggtgatcagg aagtgctgcc
    10981 cgagcgaggg cacggccgac agggcgaggc tcggctgcag ttcggcgagg ccgacgaatg
    11041 cgatcggcaa cgtgggcacc agcatcacca cgccgagcca ggtctgcgcc tcgcgatagc
    11101 tgcgcgtgaa cgatgcgacc acggtcatga gccccgccgc tgccggcgcg agcggcgcgg
    11161 tcacgaggat gatcttcagg accaccgacg catcgagatg cgcggacatg ccgaaattct
    11221 cgagcgcgac gaaccgcagc gccagggcgc aggcgatcgc ggtgagcgtc agcgagacga
    11281 tcatgaaggt ggtcgccgcg aggattttcc cgtagatcag ccgcgcgcgc ggcaccggtg
    11341 tcgtgagcag cggctcgagc gagtggcgct cgcgctcccc ggcggtcgcg tcgatcgcga
    11401 ggtacagacc gccgaggatc atcgcgagca gcagcagata gctcagcatg ccgagcaccg
    11461 tgaccgagcg gctctgcggc gtcgacacgt cgatgtcctg taccgcgagc ggtgccgtca
    11521 gcaacggatc gacaccgcgc gcgagcagcc gcagccgccc gatgctcgcc ccgtactggg
    11581 cgatcagcat ccgggtgcgc gcagcgttgc ggtccgcgcg gccgcccgcc gcatcactgt
    11641 agagctgcag cggcgcgggg cgcgcggcag cgagccgctc ggcgaaatcc gccgggatcg
    11701 acagcacgag cggatggctg cgctcgcgta cggcgcggcg cgtcgcctcg tcatcgagct
    11761 cgaccggcgt cacgatgaca ttgcgcgcct cgagccacgc gacgaggttc ggcgcgcgct
    11821 cgacgtgccg cacggcgatc tcgagacgct ggtcggcctc gtcggcggtg cgctggacga
    11881 ggaactgcag cgcggccgcg aggatcagcg ggccgaacag cggtccgaac agcagggccg
    11941 agacgagcgt gcgccggtcg cgcaggttct cgcgtacttc cttgcgccat acgatgccga
    12001 ccgcgcccat cagccgaggc cctcgtcggt accgatcagc ttcacgaacg catcctcgag
    12061 cgaggcgcag ccggtggctg cgcgcagcgc gtccggcgta ccgtgtgcga ccacctcgcc
    12121 gcgcgccagc acgaacacca cgtcgcacag gcccgagacc tcctgcatca cgtggctcga
    12181 gaacagcacg cagtgcccgg catcccgcag ccgcgcaagc agcacccgca ggttgcgtac
    12241 ggccatcacg tcgaggccgt tggtgggctc atcgagcagc acgttgcgcg ggctgtgcac
    12301 cagcgcgcgg gcgagcgccg tcttgatgcg ctggccctgc gagtagccct tggcgcgccg
    12361 cccggcaaag gcgcccatct cgagccactc gatcagctca tcggcacgcc gctcgcgtgc
    12421 cgcgcgcgcc atgccgtgca gttcggcaaa gtacaggatg ttctcgcggg ccgtcaggtg
    12481 cgggtaaagg cccgaatcgt gcgtcagcat gccgagctgg cggcgcgcgt cgagcgcgtc
    12541 gcgcaccgtg tgcgccgcgt cgatccacgc ctcgcccgcg tccgggcgca acaccgtgca
    12601 cagcatgcgc agcgtggtcg acttgcccgc cccgttcggc ccgagcaggc cggtgatcgc
    12661 gccgtcctcc gcgcgcaggg tcacgccgcg cacggcctgt acgccgccaa aggccttggc
    12721 gagtccctgc gccgacatca tgcggcgccg atcacggcgc ggggcctgcg agcgtggtga
    12781 agaacggcat cggacgcagg cgctcgacgc aggcggcgtc gagcccttcg gccgtgccgc
    12841 tcgtgacgaa cgcggcgagc agccgatcga cgcagggtgc gccgatctgc ccgtgcccga
    12901 atccctcgac gatcacgtgc cgcgaatgcc tgaatccggc cgccgcgtgc gcggcacccg
    12961 cgggcggggt caccggatcc gcgctgccgg acagcagcag tgccgggacc tcgctcgcga
    13021 gcggcgcatg caggtcatcg tcgatgacgc cacgtggcca tatctcgcac agcgccgcga
    13081 ggccgtcgag ctggcgcgta ccgatgaacg tcgcggcgag caccacgcga tcggcgcgtg
    13141 tatccgggaa ccacggcgcg tcctcggtgc agacgacgct gtggtgcatg ccgtaggcca
    13201 tctgtccgct gagcgaatcg ctcatcaaca ggaactggct cgcgagcggc acgaagttgc
    13261 ggtgcatgtg tgcctcgtac agcgcgagcg gcaggagcgc cgcctgttcg gcgccgtagg
    13321 tggacaggcg caacaccgtc gcgagatggg ccgggccgaa ttcgaccgcg acccgctcgc
    13381 cggtgcgcgg cgcaggaagc agcattcccg ccggctgctc ttcgagccgc gagcgcagcg
    13441 cccgatagtg caccaccgga tcgccgaagg cgccgcggca cgcagcgtcg ttcgcgcagc
    13501 gtgccagcac ctgtgcgagg gccgcttccg cgtcgaccgc gatatcgggg ccgaggatgc
    13561 gctcggcggg cacggtcccg tcgagcgtca ccgtgcgcac ccgcgccccg tggtgacgca
    13621 ggtagtgctg cgcgaccctc gtgccgtagg acacggcata gagattgatg cgttcgtagc
    13681 cgagcgcgcg gcgcgcgaac tcgagatccc gaaccgcggc gctggtcgtg tactcgcgca
    13741 cgtcggcgcg cgtcgagacc tcctcgagac agcgacgcgt ctgcgccagg aaccgtgcgt
    13801 cgtcggcgcc gagcagggcc tcgtcgtcga aatcgcagtg cagcgcgttc gagcgtccgg
    13861 tgccgcgctg gtcgatgagc acgatgtcgc gatcgcgatt gacccgcgca aaggccggcg
    13921 cgacgcccgc gtagaactcg ctcgccgccg ctcccggtcc gcccgcgagg acgaacagcg
    13981 ggtccggcgt cttgcgggcg ttgacggccg ggacccgggc gacgaacagg ccgatccggc
    14041 ggccatcggg gtcggcggcc ctctccggca cctgcagcgt cgtgcattcg gcggcgaccg
    14101 tggccacccc ggcggggtgg gtcagctggc aaggggccca tgccacggcc aaggcgagcg
    14161 cggtcagcat gcgggcaata tgccgtacaa taggcgtttg ccatcaagcc attgaagtaa
    14221 atggatttct tcgacgtcat agtgatcggc ggcggtcatg ccggcaccga ggccgcgctc
    14281 gcggcggccc gcatgggccg gcgcacgctg ctgctcacgc agagcctcga gacgctgggc
    14341 gcgatgagct gcaacccggc gatcggcggc atcggcaagg gccacctcgt gcgcgagatc
    14401 gatgcgctcg gcggtgcgat ggcgcgcgcg atcgatcgcg ccggtcttca gttccgcacg
    14461 ctgaatgcga gcaagggtcc cgcggtgcgc gcaacgcgag cccaggccga tcgcgtgctg
    14521 taccggcgcg agatccgcgc gctgctcgag aaccagccga acctcagcct gtttcagcag
    14581 gaagcggccg acctcgtcgt cgagggcgag cgcgtgtgcg gcgtcgtgac cgtcaccggc
    14641 atcgtattgc gcgcgcgcgc cgtcgtgctc acggtcggca cgttcctcgg gggccgcatt
    14701 cacgtgggcc tcgacaacta ccagggcggc cgcgcgggcg atccgccctc gaatcgcctc
    14761 gccgcgcggc tgcgcgagct gccgctgcgg gtcggacggc tgaagaccgg cacgccgccg
    14821 cgcatcgacg ggcgcacgat cgatttcacg cgcctcaccg aacagtggag cgacgcaccg
    14881 gcaccggtgt tttcgttcct cggccgcgcg agcgagcatc ctgcgcagcg cccctgccac
    14941 atcaccgcga ccaacacgca gacgcacgac atcatccggg ccgcgaccga tcgctcgccg
    15001 atgttcacgg gcgtgatcga gggagtaggc ccgcgctact gcccttcggt cgaggacaag
    15061 gtcgtgcgct tcgccgacaa ggcctcgcac caggtgtttc tcgaacccga ggggctcgac
    15121 acgcacgagg tgtatccgaa cggcatctcg accagcctgc cctacgacgt gcagctgcgg
    15181 ttcgtgcata cgatcaaggg cctcgagcgc gcgcacctga cccggcccgg ctacgcgatc
    15241 gagtacgact acttcgatcc gcgcgacctc gccccgagcc tcgcgacaaa ggcacttgcg
    15301 gggctttatt tcgccggcca gatcaacggc acgaccggct acgaggaggc cgcggcgcag
    15361 gggatcgtgg cgggcatcaa cgccgcgcgc gccgcgcgcg aggaggcgcc gtgggtgccg
    15421 cagcgcgcgg aggcctacct gggcgtgctg atcgacgacc tcgtcacgcg cggggtgacc
    15481 gagccctatc gcatgttcac gagtcgcgcc gagcaccgcc tgctgctgcg cgaagacaac
    15541 gcggacctgc ggctcacgcc ggtggcacgc gagctgggtc tggtggacga cgaacgctgg
    15601 gccttcttcg aggccaagcg ccgcgcgacc gatgccgagg tcgcgcgact cgagcgcacg
    15661 cgggtgcgcg cggtgagcgc gatggagctg ctgcgccgtc ccgaagtgag ctacgagggc
    15721 ctgctcgcgc agatcggatt gcccgctgcg gacgcatacg acgacgagcg catcgcgcgc
    15781 gaggtcgccc tgcaggtcac ggtgcgcgcc aagtacgccg gctacatcga gcgccagcat
    15841 gcggaggtcg agcgccagcg cgcgcatgag accgcggccc tgccggccga cctcgactac
    15901 ctcgcgatcg gcgggctctc gaacgaggcg cgccagcgcc tcgcggaagt gcgcccggcc
    15961 acgctcggcc aggccgcccg cgtgccgggg atcacgccgg ctgcgatctc gatcctgatg
    16021 atccacctga agcgccgcgg gcgtgcggcg tagtctcgcg ctcgtcctgg gcgcgttcgc
    16081 gctcgccgcg ctcggtggct gcgcgccacc gccgcccgag acctcgaaca tcctcctgcg
    16141 cggcaacggc ccggagcccg actcgctcga tccgcagaag gcgcgctcgg tcgaggccct
    16201 caccgtgctg cgcgacctgt acgaatgcct gacctcgctc ggcagggatg ccgaaacggt
    16261 gccgggcgtt gccgagacct gggccgcgag tgatgacggg ctgacctata cgttccgcct
    16321 gagagacaat gcgcgctggt ccaatggaga tagtgtcaca ggcgaggatt tcgtcttcgc
    16381 gctgcggcgc ctcgtcgacc cggcgaccgc ctcgcaatac gcccagctgg tcgatgtgat
    16441 cgacaacgcg gcggagatcg tcgccggccg cgccgccccg gaggcgctcg gggtcagtgc
    16501 gccggacccg cacaccgtcg tcgtgaggct cgcctcgccg gcgccctacc tgccgggcct
    16561 gctgtcgttt ccgtcgacct gcccggtgca ccgcccgaca ctcgaaaagg aaggcgcgcg
    16621 cttcacgagg ccgggcgtca tggtgtcgaa cggcgccttc cggctcaccg aatgggtgcc
    16681 gggttcgcac atcagcgtgg tgcgcaaccg cgcgtactgg aacgacgccg cgactcgcct
    16741 cgagggcgtg aagtacctgc agattgccga cgagagcgcc gagttcacgc gctaccgcgc
    16801 gggtgacctg cacacgaccg ccgtggtgcc acgtggccag ttcgacgtga tccgcaagga
    16861 ccatgcggcg gagctgcaca tcggcccgca gctcagcacc tactactacg gcttcgcgct
    16921 cgaccggcca cccttccgcg gcgcacccgg actgcgccgc gcgctgtcac tcgtgatcga
    16981 ccgggagcgc ctcgccgagt cggtgctgcg cgtcggcgag ctgccggcgt acggatgggt
    17041 cccccccggc gtgcaccagt acacctcgca gaccttcggt tacgcggggc tcccgatggc
    17101 ggagcgcatc gaggaggcgc ggcgcctgta tgccgcggcc ggctactccg ccgcgaggcc
    17161 gctgcggttc gagctgcgct acaacagcgg cgaggtgcac agccggctgg cggtggcggt
    17221 cgcgtcgatg tggaaggaag cgctcggcgt cgaggcgcag ctgaccgcgg tcgaattccg
    17281 ctcgctgatg gcggatgtcg atgcgcgcaa cgtcgagatg ttccggctga gctggagcgg
    17341 cgattacaac gatgcctaca ccttcctgca gtacctgaag agcgatttcg gcatcaacct
    17401 gccgcgctac gccaatcccg cctacgacgc gctgctcgcg cgagccgcgg gcgcgtccga
    17461 tgcgacggag cgccggaatc tcctcgagac ggccgagcgc atgatgctcg ccgacacgcc
    17521 gctgctgccg ctctatttct acgtgaacaa gcacctcgtc agcccgcgcg tcgagggctg
    17581 gtacgacaac gtcatgaacg tcgtctacag caaggacctg gcgctcgcgg cgccctgacc
    17641 cgccccgtgt cgaggatgcg cccgcggttg ccgggctgcg ccttgaaccg ggcgagctgc
    17701 accggtgccg agatgagcca cgccagcgca ccctgccaca ggaagacctt ggtcagcgca
    17761 accgcatcag ggggaacttg gcacccaggg gcgcggttcg cagcgcacct ccgtcttcac
    17821 cgagttggcg atgaagcact tgtcgtgcgc ctcgtcgtgc atgcccgcga tctcgtcatc
    17881 cgtggggagc ttccctccgc tgaactgcac cgccgggtgc aggatgaccc gtgtcatcgc
    17941 catgcgcccg gccgcatccc ggcccagccg gccggcggcc tcgtcgacat agcggtcgac
    18001 cacgaagccg cgctccgcgg cgatcgaaag gaaccacagc atgtggcagc tcgagagact
    18061 cgcgatgaac gcctcttccg ggtcgaccgc ggcctcgacc gaatgcggca gcggcaccac
    18121 gtgcggggat gcggagccgg gcacctccac gccgccgtcg aagcgccagc gatgggcgcg
    18181 gctgtagcgg ttgtcggtga atgtggcgcc ctcgcgcgcc cagtcgatcg tcgccttgtg
    18241 ctcagccaag aaagactcca gccgcagtta ttgttgcttc tgtgaattta tccaggcatg
    18301 gacctgctcg gtaagcacgt ccattggaac agggccgctc gcgagcacgg tgtcgtggaa
    18361 ggcgcgaatg tcgaaccgcc cgccgagcgc cgcttgtgcc tcgcgccgca ggtcgaggat
    18421 gcgcagctgg ccgatcttgt aggcgagcgc ctgtccgggc caggcgatat agcggtcaac
    18481 ctcgttgacg atgtccgcct cggtcttcgc ggcgttcgcc ttgaagtagt cgatcgcctg
    18541 ctggcgcgtc cattgcctgg catggatccc ggtgtcgacg acgaggcgca cggcacgcca
    18601 catgtcgtag gtgagctggc cgaacttgtc gtacgggtcg tcgtagagcc ccatctgctc
    18661 gccgagccgc tcgctgtaga gcgcccagcc ctcgacataa gcggtgaagt cggcattgcg
    18721 ccggaacagc ggcagcgtcc ggtcctccat cgcgagagcg atctgcaggt ggtggcccgg
    18781 caccgcctcg tgcaccgtca gtaccgggat ctcgtacttc ggccgcactt cggggcggta
    18841 gaggttcacg tagtagtagc cggcgcgcga gccgtcgatc gccgggccct ggtagtaggc
    18901 ggtggtcgtg tccggggcgc tggtcatcgg gatctcgcgc acgccgtagg gcgtgcgcgg
    18961 cagcttgccg aacagcttca cgagttcggg gtcgatgcgc ttcgccatgg ccgcgtaggc
    19021 ggtgaacagt tcctcgccgc tcccgtaata gaactgcgga tcggtgcgca ggaaggcgag
    19081 gaacttctcg aacgtgccct ggaaccgcac tcgcgcgatc acttcgtcca tctcggcacg
    19141 gatccgagcc acctcgcgca gcccgatctc gtggatgtcg tcgggcgtga ggtcggtggt
    19201 ggtatggaag gcggtgcggt tggcgtagaa cgcgagcccg tccggggtat cccagatgcc
    19261 gtccgagctg cgcgcggccg gcaggtactc gtcgcggaag aaggcgtcga gacgcttgaa
    19321 cgccgggatc accacctcga cgatcgcgcg tccgccggcg ccgatcaggc gggcgcggtc
    19381 ctcctcgccg atgtcctcgg gcatgcgcgt gaagggtgcg aagaacggac tgtcctcggc
    19441 gttcgccggg atctggttcg cgagctgcgc cgggatccgc tgcatgattg aacggggctg
    19501 ggtgcgcccc tcgcggatcg cggtcgcgag catcgcgatg tgctggtcga tataggtgcc
    19561 gaatttcgtg agccgcacga tccaggcctc gtagtcggcc acggttgcaa acggcagcag
    19621 ctcggcgagt tcgctcgcgc tctgcacgcc gtcgcgcggc cggatcgcct cgtagagcca
    19681 cggcttgaag ggctgcgccg cgagccgccg ctcgtattcc tgcgcaaaca ggtcgtagtt
    19741 cagccggtcg gcgcccgaca ggccgtcgcg tggaatggcc ttgagttcgg ccatcactgc
    19801 ggcgtagcgc gcatagcgtg cttcgagcgc ggcggccgac agatccggcc attggtccga
    19861 gtagcggtga tcgccgaggt agtcggcggc ggtggggttg tcgcgcaggt ccgcttccca
    19921 cgctgcatcg aacagggcct gcaggcgggc atcggtcgcg agcgaggtgc cgggtgccgc
    19981 gatgaggctc gcggcgagca gccagaggag cttgcgcatg cgccgattgt gcggcaatcg
    20041 cgccgtggcg caactctcag gccggatcta gccgcagctg tcccatgctg ggccgaaagc
    20101 gcagcctgcg cgccacgccg tactcgacct cgtgcgacac gatccgcacg cccgcgtcga
    20161 tcagatactc gccgatcagc gcgagcaccg cggcggcggc ctggcgcgcc ggctcgcccg
    20221 attcgacgct ggcggcaatc ttgccctgca cgtcgacgcc ccgctccatg gcgtcccgga
    20281 agccctcgac gcaacggtcg agcagcagca ggtccgaggc gagggtgcgg tcggtgacgt
    20341 cgccctggtt cgcgccgcgg gcgatgaagc gcaggtgcag gagtgccccg tgctcctcga
    20401 ccgcgtcgta caggcacacc gccagcgtcc cgtcgaggag cgccgaaaaa aggacatcat
    20461 ccgacgccac atcgaagtgg tcgggcggga cctcgactcg gcgcggatcg gggtgcagca
    20521 caccgcgcat ggtgcgggtt tccggcggcc ggaaccttga actgcatcac gggcggcaca
    20581 tggctagaat ggtgcccgcg gcacactgcc gccgattcgc gacacgcaag gggactcggc
    20641 aatgctcgat atcggctttc tgcccatcat cattctcgtc gcgatcatca tcgtcgcctc
    20701 gaaggcctgg ctgaccgtgc ctcagggtta cgagtacacg ctcgagcgct tcggcaagtt
    20761 caagaagtcg atggaaccgg ggttccactt catcgtgccg ttcgtcgagg ccatcggccg
    20821 caagatcaac atgatggagc aggtgctcga cgtgccgcgc cagga
//
LOCUS       DAOQVY010000036        20498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_3057_length_20498_cov_5.859610, whole genome shotgun sequence.
ACCESSION   DAOQVY010000036 DAOQVY010000000
VERSION     DAOQVY010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 20498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20498
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3057_length_20498_cov_5.859610"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PKN91_05140"
     CDS             complement(<1..461)
                     /locus_tag="PKN91_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HNS27494.1"
                     /translation="MTEARTDIADLMARIGRAARAAAPALALAGTTRKNAALAAAAAA
                     IRAQSATILAANAEDLAAARARGLSAAMLDRLALDPARVESMALGLDAIAALPDPVGA
                     IVGQWQRPNGLEISRVRVPIGIIGVIYEARPNVTADAGALCIKSGNVVVLRG"
     gene            complement(454..1620)
                     /gene="proB"
                     /locus_tag="PKN91_05145"
     CDS             complement(454..1620)
                     /gene="proB"
                     /locus_tag="PKN91_05145"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNS27495.1"
                     /translation="MHTRRPVSPASPLAKARRIVVKVGSSLLVDGATGRLNRAWLETL
                     AADLVRMRRRGQQVILVSSGAIALGRRQLGLPAGALRLEASQAAAAVGQIRLAHAYKE
                     VFDAQGVTVAQVLLTLEDSERRRRYLNARATLNGLLGLGVVPVINENDTVATTEIRYG
                     DNDRLAARVAQMASADCVVLLSDVDGLYTADPGVDADAQFVERVMRVTPAIEAMAGTA
                     RSDFGTGGMATKVAAARIAVSAGAHLCIAAGAHRHPLRRIERGARCTWFFPQGNPRTA
                     RQQWIAGSLRPKGAILIDEGALRALRSGKSLLPAGVTGVRGRFDRGDSVSVITPDGQE
                     FARGIAAYADGDVTRIMGRKSRELAELLGYRGRDEMIHRDDLVVVAEEAPTEND"
     gene            1732..2067
                     /locus_tag="PKN91_05150"
     CDS             1732..2067
                     /locus_tag="PKN91_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27496.1"
                     /translation="MSRDYEPTPGQWYENVEEEETFRVLTVDEDSELIEIEYLDGEIE
                     EIDLETWHEMDLDKVSEPEGWADRDDDDDDDDDDDDDDDDDDDDDDDDDDDWDDEDDD
                     WDDDDDEET"
     gene            2165..3031
                     /gene="htpX"
                     /locus_tag="PKN91_05155"
     CDS             2165..3031
                     /gene="htpX"
                     /locus_tag="PKN91_05155"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HNS27497.1"
                     /translation="MKRILLFLATNLAVIVVLSIVARIFGVDQYLMARGGSLGGLLVF
                     AAIFGFGGALISLAMSKWMAKRAMGVRVITQPANATEQWLVNTVRAQADQAGIGMPEV
                     GIFDSPQPNAFATGARRDAALVAVSSGLLRAMNQKEAEAVLAHEISHVANGDMVTLTL
                     IQGVVNTFVIFLARVIGGLVDRREDGRGMAYFATVIVAEIVLGIFASMIVMWYSRHRE
                     FRADAGGARLAGTTNMISALERLKAGQPQPLPAQMAAFGIAGGRGAGLRKLFMSHPPL
                     DERIEALRTARL"
     gene            3036..4031
                     /locus_tag="PKN91_05160"
     CDS             3036..4031
                     /locus_tag="PKN91_05160"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNS27498.1"
                     /translation="MNEATTAGAAAGGAGRMLRAIVAWFDSGDVSPARAGAGAGAAGD
                     DRIDLARIIPFIGMHLACLFAFSVGVSPVAILVAVIAYFARMFAITGFYHRYFSHRSF
                     KTSRIGQFVFGVLGAAAVQRGPLWWAAHHRVHHAYSDRKEDPHSPLQHGFLRSHMGWF
                     LTKRGFVPDLKRVRDLLQFPELRWLDRFDIFVPVLLAIGMFALGALLERVAPHLGTNG
                     WQMLVWGFFISTVACYHGTYTINSLSHVFGRQRYRTGDSSRNNWLLALVTLGEGWHNN
                     HHHYPSAARQGFYWWEIDITYYMLKLLSWLGIIWDLKPVPISARDHSARRIKSPR"
     gene            4119..5552
                     /locus_tag="PKN91_05165"
     CDS             4119..5552
                     /locus_tag="PKN91_05165"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNS27499.1"
                     /translation="MSPVITEKVALAGFGRIVGDDAVLTDADATAPYLVDHRKLYEGK
                     ALAVLRPSTVGEVSQLLAFCNGTRIGVVPQGGNTGYCGGATPDDSGRQVVLSLGRMAR
                     IRSVDAVNYSLVAEAGCILGDLQKAALEVDRYFPLSLGSEGSCQIGGNLSTNAGGLNV
                     VRYGMTRELVLGLEVVLADGRVLSTLGELRKDNTGYDLKSLFVGAEGTLGVITAASLK
                     LSPRPRALATAFVAVRSVAAAVDLLNLLRETSGDRVSSFELIPNIAIELTTRHIPGVA
                     QPLAGEYAWCVLCELSSSRATDEVDAMLESALSDAMDGGLLEDAALAQNERQRAAFWR
                     LRESIPEAQRIEGPSLKHDVALPIGALPRFVAEASAWIAERLPQAVLVAYGHVGDGNL
                     HFNVNARRGADPVAFVASADELHRHVHDLVTSLGGSISAEHGIGRLKVDELARYAPPL
                     ELELMRSIKCALDPNGIMNPGKVLKAI"
     gene            5549..5719
                     /locus_tag="PKN91_05170"
     CDS             5549..5719
                     /locus_tag="PKN91_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27500.1"
                     /translation="MTGSESSWPRPAAAWYRVAVMMIACILSFIDRVLVGPIRAEVAA
                     IATADRLAGRSS"
     gene            5720..6682
                     /locus_tag="PKN91_05175"
     CDS             5720..6682
                     /locus_tag="PKN91_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNS27501.1"
                     /translation="MSTAGRCAHLAGAVLVLAGCGRQAEPPSGPPAGAAAPAAQRMRI
                     ADGAPRIAHAPDRVHIDYRVYGQGEPLIVLIHGWSGDASYWRAQVADLSRTYTVVTPN
                     LAGHGASGRNRASWSIEAFGDDVAAVLAELPEGPVVLVGHAMGGVVALETARRAPTRV
                     IGIVGVEAFDDIGAPRRAGAEAAAAARVARLREDYIGTVRELVSQQYFAPDANPAFAR
                     SIADDIALAPPEVAIASLIAMDRYDYGPALAELPAPLVAILSSLRGAPDIARIRSQVP
                     GFRAEIMRGRGHFLMMEDPAGFNALLRREVDAFVAATPGAVAVQ"
     gene            6679..7140
                     /locus_tag="PKN91_05180"
     CDS             6679..7140
                     /locus_tag="PKN91_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HNS27502.1"
                     /translation="MTGDAHYQRYRQLSVLLDQAFRVPGTRWRFGFDALIGLVPGIGD
                     FAGALFGAYGVLVARRMGAPLMLQVRMLGNVLIDATLGAVPLAGDLFDFAFKTHKRNV
                     TLLEDWQRDPQRVVRDTRLMLIVVPLAIVLIAIVSLALAVAGLVALLRWLG"
     gene            complement(7152..7571)
                     /locus_tag="PKN91_05185"
     CDS             complement(7152..7571)
                     /locus_tag="PKN91_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNS27503.1"
                     /translation="MLRAILSTVTLAVVLSAQALAADAAPTEAQLKRGKLMFIQCQAC
                     HQLKAGAPASVGPNLNGVVGRKAASAPDFNYSPALKKANLTWDVATLDKWLERPGALV
                     PGNTMAFAGMAKAEDRTALIAWLSVEAAPATAAAAKK"
     gene            complement(7643..8623)
                     /locus_tag="PKN91_05190"
     CDS             complement(7643..8623)
                     /locus_tag="PKN91_05190"
                     /inference="COORDINATES: protein motif:HMM:NF017966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0017009 - protein-phycocyanobilin linkage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpcT/CpeT family chromophore lyase"
                     /protein_id="HNS27504.1"
                     /translation="MNALRPLALVAIAVVASGIASATDADEVAVLASWWPGIYDTSEQ
                     LVFSRGGDSARAEGSELRVRTIVARIEVPWLGEHVLYTEEFLHDEPERLRRQVLLRLA
                     PNPGGGVRVRQYTLRSPLDFRHLHRSPRQIAGLRLDDLEAIVGCDLVLRREVTQFVGG
                     TEGNACRDDAADEARYIDYRLLVGEDLYWYRKRRYFIAGNELEHEIAGFTWFPLYQAR
                     LFTCRVRWSPSGRKAELRDLHSIDLHDQGGRARFRTPEGRDLEVEMHAEDWPFAGGRD
                     ALILMLSEAGAAEPLATSWTRIDADAITLALGWINLRCAAIAPDRPAVAS"
     gene            complement(8620..9486)
                     /gene="tesB"
                     /locus_tag="PKN91_05195"
     CDS             complement(8620..9486)
                     /gene="tesB"
                     /locus_tag="PKN91_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HNS27505.1"
                     /translation="MDLRVAELLREFELERIEVNLFRGESRDIGAPQVFGGQVLGQAL
                     MAAYATVESHDVHSLHAYFLRRGDFNAPIVYHVDRARDGHSFDARRVVAIQHGEQIFN
                     LSASFQVREPGLEHQVPMPDVPPPEELTDDGTPPAAVLEQLPEKQRRMFEQQRPFEFR
                     VVEPFDYVRPQQMAATQHVWLRAVGRLPDDEHLHRCLLAYVSDYSLLATAVRPHGTAY
                     LSGQLIMASIDHAMWFHRSPRVDDWLLYSMDSPSASGARGFARGTLHSRDGRLIASTA
                     QEGLIRVAKPQQ"
     gene            complement(9492..10538)
                     /locus_tag="PKN91_05200"
     CDS             complement(9492..10538)
                     /locus_tag="PKN91_05200"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNS27506.1"
                     /translation="MNPINAEVVIIGAGPCGLFQVFELGLLGISAHVLDSLRAPGGQC
                     TELYPDKPIYDIPALPVCGAQELIDRLMQQAQPFHPQFHLGSEVCELRRLDSGRFHVR
                     NAAGTTFDAGAVVIAAGVGSFQARQIGVEGAAQFEGKQIHYRVRNAADFLGKRLVIFG
                     GGDSALDWVNEFAGKGTFVTHVHRRAEFRAAPASVARMRELESAGKLRHIEGLAHALI
                     VDGAGTDAAKLRGIEVKARDGTMHALEADHVFAFFGLHPKLGPIAEWGLELEKKALKV
                     DTEKFQTSVPGIFAVGDINTYPGKKKLILSGFHEAALAAFAIQHHLYPAKKQFLQYTT
                     TSPAIQKRLGVTGS"
     gene            10662..11129
                     /locus_tag="PKN91_05205"
     CDS             10662..11129
                     /locus_tag="PKN91_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNS27507.1"
                     /translation="MLTINVNGTDRAVDIDPETPLLWVLRDAIGLTGTKFGCGMALCG
                     ACTVHLDGRPVRACVTPVSAVGTAKITTIEGLSADRSHPVQRAWIELDVPQCGYCQSG
                     QILSATALLAGNPRPTDADIDAAMSGVICRCGTYQRIRAAIHRAAELAAETRS"
     gene            11126..13276
                     /locus_tag="PKN91_05210"
     CDS             11126..13276
                     /locus_tag="PKN91_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNS27508.1"
                     /translation="MNARPGWSALSRRDFLKSSAVGVGGLVLAIDLAGCSKPGATDGE
                     TRIIESNAWLRIGTDNSIHFLCDRSEMGQGVYTGLVTLLAEELEVDPQRIQVAFAPAG
                     AAYVNALLGGQITGGSTSLRDGWEKLRMAGAQAREMLKTAAAREWKTTASSCRVENGE
                     IISSFGKRYTYGEMAEAAAALPVPTDLRLKDPDAFRWIGKPSARLDTPAKVDGSAIYG
                     IDVRLPGMLYASLAMPPMLGGMARSVDDAAARAMPGVQAVVPTSSGIAVVADSWWQAL
                     QARSALKVEWDGSASTALNDGAILRGLKKAAAGNGIIARQEGDADAALATAAKVVRAE
                     YQLPMLAHATLEPQVCTADVRADGCDIYLPTQAQGAAQAAAAHAAGLEPAQVNVHTTF
                     LGGGFGRRLEVDFVPAAVEASKAVGKPVKLLWTREDDTTHDFYRPPAYDTVAGGLDRD
                     GKLVAWKFHIVGPSITARVFPGAVPPGSVDPFAVEAAANYPYDVPNVLVDWVPHEVGL
                     GVGYWRSVSHALNCFVAESFMDELALAAGQEPAAFRRALLAKQPRYLGALEVALRRSG
                     YGKDQPGRFRGIAVMEGYGTYMAQVAEISLEDGKVKVHRIVCAVDCGRAVNPTIVVAQ
                     VEGAILFGLSATLWGEINVQGGRVQQSNFHDCRVVRMNEVPVIETHLIESDEPPGGIG
                     EPATALVAPAICNAIHAATGRRLRSLPLARHNLA"
     gene            13273..14589
                     /gene="gabT"
                     /locus_tag="PKN91_05215"
     CDS             13273..14589
                     /gene="gabT"
                     /locus_tag="PKN91_05215"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HNS27509.1"
                     /translation="MSSNSDLQKRREAAIPRGVGNSTAIYADYARNAEIRDVEGRRYI
                     DFASGIAVLNTGHRHPAVEAALEAQIGRLTHACFQVTPYESYVALAEALNALAPGATP
                     KKTIFLTTGAEAVENAIKIARYHTRRSAVIAFAGGFHGRTLACSALTGKVMPYKAGFG
                     PMMPEVFHAPFPIAYHGVSADDSLAALEQLFKADVDPARVAAIIIEPVLGEGGFYVAP
                     ADFLRRLRALCDRHGILLIADEIQTGFVRTGRMFAIEHAGIEPDLMTVAKSLAGGVPL
                     SAVIGKAEIMDAPAPGGLGGTYAGSPLGCAAGLAVLEVIEREQLAARAVVLGERIVTR
                     LKALQARHACIGEVRGLGAMVAMELVRNRKADAPDAELTKQLVQAAARRGLVILSCGI
                     HSNVIRFLAPLTIEDALLEEGLEILEAALDEVTAPAAGLERQAQGS"
     gene            complement(14586..14885)
                     /locus_tag="PKN91_05220"
     CDS             complement(14586..14885)
                     /locus_tag="PKN91_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27510.1"
                     /translation="MSELGNSFDAAFSKAVDLGNKIADKDKDADLWDIADGLLAGAVQ
                     YWLYSRQPCGDPRCQDCMPISTATGRMAELKRLMEELASESEYFHTPTDSNAGRA"
     gene            complement(15027..16028)
                     /locus_tag="PKN91_05225"
     CDS             complement(15027..16028)
                     /locus_tag="PKN91_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNS27511.1"
                     /translation="MSGEIVLTTLAMLVVLALLFDFMNGFHDAANSIATVVSTGVLKP
                     YQAVVWAAMFNFVAIGIFHLKVATTVGKGIIDPDFVDYHVIFGALVGAITWNVITWYY
                     GIPSSSSHALIGGMIGAAMAKAGTAPLLAPGIIKVSAFIVVAPMLGLVLGALWMVAVS
                     WICLRFSPHRVDRVFRRLQLVSAALYSIGHGSNDAQKTMGIIWLLLIAAGETTRDHLP
                     VWVIISCYAAISIGTLFGGWRIVKTMGQRITKLRPVGGFAAESAGALTLFGATSFGIP
                     VSTTHTITGAIIGVGTAQRASAVRWGIAGNIVLAWLVTIPASAFFAAIAWWIGMRMF"
     gene            complement(16031..16672)
                     /locus_tag="PKN91_05230"
     CDS             complement(16031..16672)
                     /locus_tag="PKN91_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNS27512.1"
                     /translation="MNLFSALMPREGQFFTLFNEHAALIVKGGRELFELLQDYGDVDG
                     RAARISRIETIEHDADRVTREAVELLHRSFVTPFDRDVIHRLISRMDDILDLIQDTAE
                     TLVLYDIQHLTAEAIHLANLVQICCERVEAAIGLLSSMKNAKEIMKISLDIDGLESDA
                     DRVMRGAVSKLFRDERDDRQLIKLKAVYELLEATTDKCQDVANVIEGVVLENG"
     gene            16774..18558
                     /locus_tag="PKN91_05235"
     CDS             16774..18558
                     /locus_tag="PKN91_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS27513.1"
                     /translation="MTRIALLDAHLAFGLLPLLDGADLHVTAGERIGLIGRNGTGKSS
                     LLAVLAGRQDLDAGEVQRRDGLSVALVEQEPDLPAAPTLGASLALRGGAERLDEAARA
                     ALTVRVTAQLDRFGLAPGQPSSGLSGGERKRAALALAFALEPGLLLLDEPTNHLDIAG
                     IEQLEALAAGAATLVVVTHDRRFLDRVATRIVELDRGVLRSYPGNFAAYERAKTQELA
                     AEAVANRRFDKFWKQEEAWIRQGVEARRTRNEGRVRRLEMLRLERSRRRERTGDVRFT
                     LAAGDRSGRLVIELDRVSVSFGARPIVRDLSMRIMRGDRIGLIGPNGAGKTTLLRLML
                     GELEPDSGTVRTGSNLRVAYFDQLRSQLDPERTVAETISPGSDWVEVGGVRKHVTTYL
                     AEFLFPSRRARSPVRMLSGGERHRLLLARLFAQPANLLVLDEPTNDLDMESLELLEET
                     LQGYGGTLLLVSHDREFLDNVVTQTLVAEGDGRWREYVGGYSDWLAQRGVAPREGATS
                     AAPVRDRTRVRQRFGYREQKELDALPAAIESLEAEKSALTAAMCAPDYHRAGPARARD
                     DAARLAAIDHDIEAAMARWEELEARRAQ"
     gene            complement(18612..>20498)
                     /locus_tag="PKN91_05240"
     CDS             complement(18612..>20498)
                     /locus_tag="PKN91_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNS27514.1"
                     /translation="PIAGTTSGEVVPVAGLACDAGSVPATVAGNIALINRGTCAISLK
                     VANATAAGATAVILGLVAPGDAVSFSNGGECSGQCVPTLVVTQAVANSIRAATNAGNV
                     VSATISEANAQSRQGWIVGTSSRGPSTSFAAIKPEIGAPGASVSAIAGSGNGEEAFGG
                     TSGAAPMVSGAAALLLSKNPSLTPTLVRALLANTADPEVYQRAPSGEDKILVPITRIG
                     GGEVRVNKALAATTAMWDAATKTPALSFGYHSVSYPKVLRRTVKVRNFSNKTRRYSVQ
                     KSFRYADDASSGAVTISAPSTVTVPANGTATFRVTLSINPAKLGDWPYTGAGGSQGGN
                     GALLQAAEYDGYLTLKNGADSVHVPWHVLPKKAALVQAIGAGVVIPQDHTSAQLPLLN
                     LAPAKAGVVEPFALLGTSARIPRSQLPGPGENFAVVDMQALGARLVGIGGGQFGLQVA
                     VSTYGTRAHPSYPAEFDVYLDVDADGVDDFVLYTAELTGFAATGQTAVYVYNLRTGTA
                     AAYFYADTDLESGNVILTAPLSVMGASPSSQMVISALAFDNYFTGNLTDYIDGKVFTP
                     GLPRFDVSAPPAAGVAPLSLTMLTVTAPEGGETASPSQEGLLFFYRDANVGLEADVVP
                     VEKR"
BASE COUNT         3263 a         7128 c         6920 g         3187 t
ORIGIN      
        1 ccgcgcaaca ccacgacatt gcccgacttg atgcagagcg cgcccgcgtc cgccgtcacg
       61 ttcgggcgtg cctcgtagat cacgccgatg atgccgatcg gcacgcgcac gcggctgatc
      121 tccaggccgt tcggccgctg ccactgcccg acaatggcgc cgaccggatc gggtagcgcc
      181 gcgatcgcat cgagaccgag cgccatcgat tcgacccgcg ccggatcgag cgcaaggcga
      241 tcgagcatcg cagccgacag gcccctggcg cgcgccgccg ccaggtcctc ggcgttggcc
      301 gcgagaatgg tggccgattg cgcccggatc gcggccgccg ccgccgcgag ggctgcgttc
      361 ttcctggtgg tgcccgccag tgcgagcgcc ggcgcagcgg cccgcgcggc acggccgatc
      421 cgcgccatca ggtcggcaat gtcagtgcgc gcttcagtca ttttcggtcg gtgcctcctc
      481 ggcgacgacc acgagatcgt cgcgatgaat catctcgtcg cggccgcggt acccgagcag
      541 ctcggcgagt tcgcgactct tgcgacccat gatgcgcgtg acatcgccgt cggcataggc
      601 cgcgatgccg cgcgcgaatt cctgtccgtc cggcgtgatg acgctcacgg agtcgccccg
      661 atcgaagcgg ccgcgcactc cggtcacgcc ggccggcagc aggctcttgc ccgagcgcag
      721 cgcacgcaat gcgccctcgt cgatcaggat cgcgcctttc ggccggagcg atccggcaat
      781 ccactgctgg cgcgcggtgc gcggattgcc ctgcggaaag aaccatgtgc agcgcgcacc
      841 gcgttcgatg cggcgcaacg gatgccggtg cgcgcctgcg gcgatgcaca ggtgcgcgcc
      901 cgcggagacg gcaatgcgcg ccgcagcgac cttggtggcc atgccgccgg tgccgaagtc
      961 ggagcgcgct gtgccggcca tcgcctcgat ggccggggtc acgcgcatca cgcgctcgac
     1021 gaactgcgcg tcggcatcga cgcccggatc ggccgtgtac agtccgtcga cgtcggacaa
     1081 cagcacgaca caatcggcag aagccatctg cgcaacccgc gcagccagcc ggtcgttgtc
     1141 accgtagcgg atctcggtcg ttgcgaccgt atcgttctcg ttgatcaccg gcaccacgcc
     1201 gagcccgagc aggccgttca gcgtggcccg cgcgttgaga tagcggcggc ggcgctcgct
     1261 gtcctcgagg gtcagcagca cctgcgcgac ggtgacgccc tgcgcatcga acacttcttt
     1321 ataggcgtgc gccagccgga tctggccgac cgccgcggcc gcctggctcg cctcgagccg
     1381 cagcgcaccg gccggcaggc cgagctggcg ccgcccgagc gcaatcgccc cggatgacac
     1441 caggatgacc tgctggccgc gccgacgcat gcgcacgagg tccgcggcga gtgtctcgag
     1501 ccaggcgcgg ttcaggcgcc ccgtggcccc gtcgacgagc agggacgagc ccaccttgac
     1561 gacgatgcgg cgggctttgg caagcggcga ggccggcgaa accggtcgtc tggtgtgcat
     1621 ggcgggggcc gaaaatccgg ggggagaacc gattcattat acggtaccgg cgcgttgcct
     1681 cggctccggg gtgaccgcat actattcgct ggcgactaca ggatccccac cgtgagcaga
     1741 gactacgaac ccacacccgg ccagtggtac gaaaacgtcg aggaagagga gaccttccgc
     1801 gtcctgacgg tggacgagga ctccgagttg atcgagatcg agtatctcga cggcgagatc
     1861 gaggaaatcg acctcgaaac ctggcatgag atggacctcg acaaggtcag cgagcccgaa
     1921 ggttgggccg atcgcgacga cgacgacgat gatgatgacg acgatgacga cgatgacgat
     1981 gacgatgacg acgatgacga tgacgacgac gacgattggg acgacgagga cgacgactgg
     2041 gacgatgacg acgacgagga aacctgacgt cgggcggccc ggtcgccctt gaaacccctc
     2101 gccgcgcccc caactcatgc tgggcgccta tgccccggct catgcacagg aacctgaacg
     2161 cgaaatgaag cgaatcctcc tattcctcgc caccaacctt gccgtgatcg tcgtgctgtc
     2221 gatcgtggcg cgcattttcg gcgtcgacca gtacctgatg gcgcgcggcg gcagcctcgg
     2281 cggactgctg gtctttgccg cgatcttcgg tttcggcggc gcgctgattt cgctcgcgat
     2341 gtcgaaatgg atggccaagc gcgcgatggg tgtgcgcgtc atcacgcagc ctgccaatgc
     2401 caccgagcag tggctggtca acaccgtgcg cgcacaggcc gaccaggcgg gcatcggcat
     2461 gccggaagtc ggcatcttcg actccccgca gcccaacgcc ttcgccaccg gcgcgcgacg
     2521 cgacgccgcc ctggtcgcgg tcagcagcgg gctgctgcgc gcgatgaacc agaaggaagc
     2581 cgaagcggtc ctcgcccacg aaatcagcca cgtcgccaac ggcgacatgg tgaccctcac
     2641 gctgatccag ggcgtcgtga acaccttcgt gatcttcctc gcccgcgtga tcggcggcct
     2701 cgtcgatcgg cgcgaggacg ggcgcggcat ggcgtatttc gctaccgtga tcgtcgccga
     2761 gatcgtgctc ggcatcttcg cgagcatgat cgtcatgtgg tactcgcggc accgcgagtt
     2821 ccgcgccgac gcgggcggcg cgcgtctcgc cggcaccacc aacatgatca gcgcgctcga
     2881 gcgcctgaag gcgggacagc cgcaaccgct acccgcccag atggccgcct tcggcatcgc
     2941 cggcggcaga ggtgcaggcc tgagaaagct cttcatgagc cacccgccgc tcgatgagcg
     3001 tatcgaagcg ctgcgtaccg cgcgcctgta agtccatgaa cgaagccacc acggcaggcg
     3061 ccgcagccgg cggcgcggga cgcatgctgc gcgcgatcgt cgcctggttc gactcgggcg
     3121 acgtgagccc ggcgcgcgcc ggcgccggcg ccggcgcggc aggcgacgat cgcatcgatc
     3181 ttgcgcgcat catcccgttc atcggcatgc accttgcgtg cctgttcgcg ttttcggtcg
     3241 gggtgagccc ggtggcgatc ctggttgcgg tgatcgctta cttcgcgcgc atgttcgcga
     3301 tcacgggctt ctatcaccga tacttctcgc accgctcgtt caagacgtcg cgcatcggcc
     3361 agttcgtgtt cggcgtgctc ggcgctgcgg ccgtgcagcg cgggccgctg tggtgggccg
     3421 cgcaccaccg ggtgcaccac gcctactccg accgcaagga agacccgcac tcgccgctgc
     3481 agcacgggtt cctgcgctcg catatgggct ggttcctgac caagcgcggc ttcgtgccgg
     3541 acctgaagcg ggtgcgcgat ctgctgcagt tcccggagct gcgctggctc gaccgcttcg
     3601 acatcttcgt gccggtgctg ctcgcgatcg ggatgttcgc gctcggcgca ctgctcgagc
     3661 gcgttgcacc acacctcggc acgaacggct ggcagatgct ggtctggggt ttcttcatct
     3721 cgactgtggc gtgctaccac ggtacctaca cgatcaattc cctctcgcac gtcttcggcc
     3781 gccagcgcta ccgcaccggc gactcgagcc gcaacaactg gctgctggcg ctcgtcacgc
     3841 tcggcgaggg ctggcacaac aatcaccacc actacccgag cgccgcgcgc cagggcttct
     3901 attggtggga gatcgacatc acctattaca tgctgaagct gctgtcgtgg ctcggcatca
     3961 tctgggacct gaagccggtg ccgatctcgg cccgcgacca ttccgcccgg cgcatcaaga
     4021 gcccgcgttg atccacttgc ggtaggatgg gcgctcatga agtgccgcca ccgcaacctg
     4081 aacctgccgg agcgcagcgc ggcgcacgca gcgggctgat gtcacctgtc attacggaaa
     4141 aggtcgcgct ggccggcttc ggtcgcatcg tcggcgacga tgccgtgctg accgacgccg
     4201 acgccaccgc cccctacctg gtcgatcacc gcaagctgta cgaaggcaag gcgctcgccg
     4261 tgctgcgccc ctcgacggtc ggagaggtct cccagctgct cgccttctgc aacggcacgc
     4321 gcatcggcgt cgtaccgcag ggcggaaata ccggttattg cggcggcgcc accccggacg
     4381 acagcggccg ccaagttgtg ctgtcgctgg gccgcatggc gcgcatacgc tcggtcgacg
     4441 cggtgaacta ctcgctggtc gcggaagccg gctgcatcct cggcgacctg cagaaggccg
     4501 ccctggaggt cgaccgctac ttcccgctga gcctcggttc ggagggcagt tgccagatcg
     4561 gcggcaatct ttcgaccaac gcgggcggac tcaatgtcgt gcgctacggc atgacgcgcg
     4621 agctggtgct cggcctcgag gtggtgctcg ccgacggccg cgtgctctcg acgctcggcg
     4681 agctgcgcaa ggacaacacc ggctacgacc tgaaatcgct gtttgtcggc gccgagggga
     4741 cgctcggggt gatcaccgcg gcgagtctca agctctcgcc gcggccgcgc gcgctcgcca
     4801 ccgccttcgt cgccgtgcgc agcgtcgctg cggcggtcga cctgctcaac ctgctgcgcg
     4861 agacgagcgg cgatcgggtc agctccttcg agctgatccc gaacatcgcg atcgaactga
     4921 ccacgcgcca catccccggc gtcgcgcagc cgctcgccgg cgaatatgcc tggtgcgtac
     4981 tgtgcgagct gagttcctcg cgcgcgaccg acgaagtcga cgcaatgctc gaaagtgcgc
     5041 tgagcgatgc gatggacggc gggctgctcg aggacgccgc actcgcgcag aacgagcgcc
     5101 agcgcgccgc gttctggcgc ctgcgcgaat cgatcccgga ggcgcagcgc atcgagggcc
     5161 cgagcctgaa gcacgacgtg gcactgccga tcggcgcgct gccccgcttc gtcgcagagg
     5221 cttcggcgtg gatcgccgag cgcctgccgc aggcggtgct ggtggcctac ggccatgtcg
     5281 gcgacggcaa cctgcatttc aatgtcaacg cgcgccgtgg cgcggacccg gtggcgttcg
     5341 tcgcctccgc cgacgaactg caccgccatg tccacgacct cgtcacctcg cttggcggca
     5401 gcatcagcgc cgagcacggc atcgggcgcc tgaaggtcga cgaactcgcg cgctatgcgc
     5461 caccactcga gctcgagttg atgcgctcga tcaagtgcgc tctcgacccc aacggcatca
     5521 tgaatcccgg caaagtactg aaagccatat gaccggatcc gaatcgagct ggccgcgccc
     5581 cgccgccgcc tggtacaggg tggccgtcat gatgatcgcc tgcatcctgt cgttcatcga
     5641 cagggtgctc gtcgggccga tccgcgccga agtggcggcg atcgccacag ccgaccggct
     5701 ggccggcagg agcagctgaa tgtccaccgc cggccgctgc gcccacttgg ccggcgcggt
     5761 gctggttctc gccggctgcg gccgccaggc cgagccgccg tcggggccgc ccgccggtgc
     5821 cgctgccccc gcggcgcagc gcatgcggat cgcggacggt gcgccgcgca tcgcacatgc
     5881 acccgaccgc gtgcacatcg actaccgcgt ctatggccag ggtgaaccgc tgatcgtcct
     5941 gatccacggc tggtccggcg atgccagcta ctggcgggcg caggtcgccg acctgtcacg
     6001 cacttacacc gtcgtcacgc ccaacctcgc cggccacggc gcgtcgggcc gcaatcgcgc
     6061 cagctggagc atcgaggctt ttggcgacga cgttgcggcc gtgctggcgg agttgcccga
     6121 aggccccgtg gtactcgtcg gccacgcaat gggtggcgtc gtggcactcg agacggcgcg
     6181 gcgtgcgccc acgcgtgtca tcggcatcgt cggcgtcgaa gccttcgacg acatcggcgc
     6241 gccccgccgc gcaggggcgg aagccgccgc cgcggcacgc gtcgcgcgcc tgcgcgagga
     6301 ttacatcggc acggttcgcg aactcgtctc gcagcagtat ttcgcgccgg acgccaatcc
     6361 ggcctttgcg cgcagcatcg ccgacgacat cgcgctcgcg ccgcccgaag tggccatcgc
     6421 ctcgttgatc gccatggaca gatacgacta cggcccggcg ctcgccgaat tgccagcgcc
     6481 gctcgtcgcg attctctcca gcctgcgcgg cgcccccgac atcgcgcgga tacgctcgca
     6541 ggtgccgggt tttcgcgccg agatcatgcg cggtcgcgga catttcctga tgatggagga
     6601 cccggcggga ttcaacgccc tgctgcgcag ggaggtcgat gcgttcgtgg ccgccacgcc
     6661 cggcgcggtc gccgtgcaat gacgggcgac gcgcactacc agcgctaccg gcagttgagc
     6721 gtcctgctgg accaggcgtt tcgcgtgccc ggcacgcgct ggcggttcgg tttcgacgcg
     6781 ctgatcgggc tcgtgcccgg catcggtgac ttcgccggcg cgctgttcgg cgcgtacggc
     6841 gtactggtgg cgcgtcgcat gggcgccccg ctcatgctgc aggtgaggat gctcgggaac
     6901 gtgctcatcg acgcgacgct cggcgccgtg ccgctggcag gtgacctgtt cgacttcgcc
     6961 ttcaagacgc acaagcgcaa cgtcacgctg ctcgaggact ggcagcgcga cccgcagcgt
     7021 gtggtgcggg acacgcggct gatgctgatc gtcgtgccac tcgcgatcgt gctgatcgcg
     7081 atcgtgtcgc tggcgctcgc cgtcgcgggg ctcgtcgcgc tcctacgctg gctcggctga
     7141 acctgcggac cttacttctt ggcggcagcc gccgtcgcag gcgcggcttc aacgctgagc
     7201 cacgcgatca gggccgtgcg gtcttcggcc ttcgccatgc ccgcaaaggc catcgtgttg
     7261 cccggtacga gggcgcccgg acgctccagc cacttgtcga gggtcgcgac atcccaggtc
     7321 aggttcgcct tcttgagcgc gggggaatag ttgaagtccg gcgccgaagc ggccttgcgc
     7381 ccgacgacgc cattcagatt cggacccacc gacgccgggg cacctgcctt cagctgatgg
     7441 caggcctggc actggatgaa catcagcttg ccgcgcttca gctgcgcctc ggtcggcgcg
     7501 gcatcagccg ccagcgcctg cgctgacagg actacggcaa gggtcacggt ggacagaatc
     7561 gcgcgcagca tcggcaaacc tcccagtggg actgcgtgca agtctactgc accaagctga
     7621 acgccagctt aagcactttc cgctagcttg cgaccgcagg ccggtcgggc gcgatggccg
     7681 cgcagcgcag gttgatccac ccgagcgcga gcgtgatcgc atcggcatca atgcgggtcc
     7741 acgacgtcgc gagtggctcg gcagcacccg cctcgctcag catcaggatc agcgcatcgc
     7801 gcccgcccgc gaacggccag tcctcggcgt gcatttcgac ttcgaggtcg cgtccctccg
     7861 gggtgcgaaa gcgcgcgcgg ccaccctggt cgtgcaggtc gatgctgtgc aggtcacgca
     7921 gttcggcctt gcgcccgctc ggcgaccagc gcacgcggca ggtgaagagc cgcgcctggt
     7981 agagcgggaa ccaggtgaat ccggcgatct cgtgttcgag ctcgttgccg gcaatgaaat
     8041 agcgccgttt gcgataccag tagaggtcct cgcccacgag cagccgatag tcgatgtaac
     8101 gcgcctcgtc ggccgcatcg tcgcggcacg cgttgccttc cgtgccgccg acgaattgcg
     8161 tgacttcccg gcgcaacacg aggtcgcagc cgacgatcgc ctcgaggtca tcgaggcgca
     8221 gtcccgcgat ctggcgtggc gagcggtgca ggtgcctgaa gtccagggga ctgcgcagcg
     8281 tgtactggcg cacgcgcacc ccgccgcccg gattcggcgc gaggcgcagc aacacctgcc
     8341 ggcgcagccg ctcgggctca tcgtgcagga actcttcggt gtagagcacg tgctcgccga
     8401 gccagggcac ttcgatgcgc gccacgatcg tgcgcacgcg cagctcggaa ccctcggcgc
     8461 gcgcggaatc cccgccgcgg ctgaagacca gttgctccga ggtgtcgtag atgccgggcc
     8521 accagctcgc gagcacggcg acctcgtcgg catcggttgc gctggcgatc ccggaggcaa
     8581 cgaccgcaat cgccactaaa gcgaggggtc gcagcgcgtt cattgctgcg gctttgccac
     8641 ccggatcagg ccttcctgcg ccgtgctggc gatcagccgc ccgtcgcgac tgtgcagcgt
     8701 gccgcgcgcg aagccgcgcg cgccggaagc actggggctg tccatcgagt agagcagcca
     8761 gtcgtcgaca cgcggggacc ggtggaacca catggcgtgg tcgatactcg ccatgatcag
     8821 ctgccccgag aggtacgccg tgccatgggg gcgcacggct gtcgcaagca ggctgtaatc
     8881 cgagacatag gcgagcaggc agcggtgcag gtgctcgtca tcgggcaggc gcccgacggc
     8941 acgcaaccag acgtgctgcg tcgccgccat ctgctgcggc cgcacgtagt cgaagggctc
     9001 gaccacgcgg aactcgaagg ggcgctgctg ctcgaacatg cgccgctgct tctcgggcaa
     9061 ttgctcgagc accgcggccg gcggcgtacc gtcgtctgtc agctcctcgg gcggcgggac
     9121 atcgggcatc ggcacctggt gctcgaggcc gggctcgcgc acctggaatg acgccgacag
     9181 gttgaagatc tgctcgccgt gctggatcgc gaccacgcgc cgcgcatcga aactgtggcc
     9241 atcgcgtgcc cggtcgacgt ggtagacgat cggcgcgttg aagtcgccgc ggcgcaggaa
     9301 ataggcgtgc aatgaatgca cgtcgtgcga ctcgacggtg gcatacgcgg ccatcagcgc
     9361 ctgaccgagc acctgcccgc cgaacacctg gggcgcgccg atgtcgcggc tctcgccgcg
     9421 gaacaggttc acctcgatgc gttcgagctc gaactcccgc agcagctcgg caacgcgcag
     9481 gtccatcgtg ctcaacttcc cgtgacgccg agacgcttct ggatcgcagg gctcgtcgtc
     9541 gtgtactgca ggaactgctt cttcgccggg tagaggtgat gctggatcgc gaacgccgcc
     9601 agcgccgcct cgtggaaacc cgacaggatc agcttcttct tgccgggata ggtgttgatg
     9661 tcgccgaccg cgaaaatgcc gggtaccgac gtctggaatt tctcggtgtc gaccttgagc
     9721 gctttcttct cgagctcgag cccccactcc gcgatcggcc ccagcttcgg atgcaggccg
     9781 aaaaaggcga acacgtggtc ggcctcgagc gcatgcatgg tgccgtcgcg cgccttcacc
     9841 tcgatgccac gcagtttcgc ggcgtcggtg ccggcgccat cgacgatcaa cgcatgcgcg
     9901 agcccctcga tgtggcgcag cttgcccgcc gactccagtt cgcgcatccg cgccaccgac
     9961 gcgggggccg cgcggaactc cgcacgccgg tgcacatggg tcacgaaggt gcccttgccg
    10021 gcgaactcgt tgacccagtc gagcgcggaa tcgccaccgc cgaagatcac gaggcgcttg
    10081 ccgaggaagt cggccgcgtt gcgcacgcgg taatggatct gcttgccctc gaactgcgcg
    10141 gccccctcga cgccgatctg gcgtgcctga aacgagccca cgcccgcggc gatcaccacg
    10201 gcgccggcat cgaaggtcgt gcccgccgca ttgcgcacgt gaaagcgccc cgaatcgagc
    10261 cgccgcagct cgcacacctc ggaaccgagg tggaactgcg ggtggaacgg ctgtgcctgc
    10321 tgcatcagcc tgtcgatgag ttcctgggcg ccgcacaccg gcagcgccgg gatgtcatag
    10381 atcggcttgt ccggatacag ctcggtgcac tggccacccg gtgcccgcag tgaatcgagc
    10441 acatgggccg aaatgcccag cagaccgagt tcgaagacct ggaacaggcc gcaggggccc
    10501 gcgcctatga tgacgacttc cgcattgatc gggttcaatc tcgcctcccc gtgaacgaag
    10561 ggaaattcta gcagcacggt agcccggggc cgcattggcc gccctcgggc caatgggact
    10621 atattgggca ccggccagca gcaagtctgc gggagatctc tatgcttacg atcaacgtca
    10681 atggcactga tcgcgcggtg gacatcgacc cggagacacc gctcctgtgg gttctgcgcg
    10741 acgcgatcgg gctcaccggc accaaattcg gctgcggcat ggccctgtgc ggcgcgtgta
    10801 ccgtccacct cgacggccgg ccggtccgcg cctgcgtcac gcccgtcagc gcggtcggga
    10861 ccgcaaagat caccaccatc gagggtctat ccgccgaccg cagccacccg gtgcagaggg
    10921 cctggatcga actcgacgtg ccccaatgcg gctactgcca gtccggccag atcttgtcgg
    10981 cgaccgcgct gctcgcgggc aacccgcgac cgaccgatgc agacatcgac gccgcgatgt
    11041 ccggcgtcat ctgtcgctgc ggcacctacc agcgcatccg cgcggcgatc catcgcgccg
    11101 ccgaactcgc cgcggagacg cggtcatgaa cgcccggccc ggctggagtg cgctgtcgcg
    11161 gcgcgacttc ctgaagagta gcgccgtcgg tgtcggcggg ctcgtcctgg caattgacct
    11221 cgccggctgc tcgaagcccg gcgcaacgga cggcgagacc cggatcatcg agtcgaatgc
    11281 gtggctgcgc atcggcaccg acaattccat ccacttcctg tgcgaccgct ccgaaatggg
    11341 ccagggcgtg tatacgggcc tcgtgacgct gctcgccgag gaactcgagg tcgacccgca
    11401 acgcatccag gtcgccttcg caccggcagg cgccgcttac gtcaatgcgc tgctcggcgg
    11461 ccagatcacg ggcggcagca ccagcctgcg cgatggctgg gagaaactgc gcatggccgg
    11521 cgcacaggca cgcgagatgc tgaagaccgc cgcggcgcgc gagtggaaaa ccacggcgag
    11581 ctcctgtcgg gtcgagaacg gcgaaatcat ctcctccttc ggcaagcgct acacctatgg
    11641 cgagatggca gaggccgccg ccgccctgcc cgtgccgacc gacctcaggc tgaaggaccc
    11701 ggacgcattc aggtggatcg gcaagccttc cgcccggctc gacacgccgg cgaaggtcga
    11761 cggctcggcg atctacggca tcgacgtcag gttgcccggc atgctgtatg cgtcgctcgc
    11821 catgccgccg atgctcggcg gcatggcaag gagcgtcgat gacgccgcgg cccgggccat
    11881 gccgggcgtg caggccgtcg tgccgacgag ttcgggcatc gccgtcgtcg cggacagctg
    11941 gtggcaggcg ctgcaggcgc gcagtgcgct gaaggtcgag tgggacggca gcgcgagtac
    12001 ggcgctgaac gacggcgcga tcctgcgcgg gctgaagaag gctgccgcgg ggaatggcat
    12061 catcgcacgc caggagggcg atgccgatgc cgcgctcgcc accgcggcga aggtcgtgcg
    12121 cgccgaatac cagttgccga tgctcgcgca cgcgaccctc gagccgcagg tctgcacagc
    12181 cgacgtgcgc gccgacggct gcgatatcta cctgccgacg caggcacagg gcgcagccca
    12241 ggcggctgcg gcacacgctg cggggctgga gcccgcgcag gtgaacgtcc atacgacctt
    12301 cctgggcggg ggcttcggcc ggcggctcga ggtcgatttc gtgcccgcgg ccgtcgaggc
    12361 ctccaaggcc gtcggcaagc cggtgaaact gctgtggacg cgcgaggacg atacgaccca
    12421 cgacttctac cgcccgccgg cctatgacac cgtggcgggc ggactcgatc gcgacggcaa
    12481 gctggtcgcc tggaagttcc atatcgtcgg accgtcgatc accgcgcgcg tcttccccgg
    12541 cgccgtgccg ccgggtagcg tcgatccgtt cgcggtcgag gccgcagcca attatcccta
    12601 cgacgtgccg aacgtgctgg tcgactgggt gccgcacgaa gtcgggctcg gcgtgggcta
    12661 ctggcgctcg gtgagccacg cactcaactg cttcgtcgcc gagagcttca tggacgagct
    12721 cgcgctcgcc gccggccagg agccggcggc attccgccgc gcgttgctcg caaagcagcc
    12781 acgctacctg ggcgcactcg aggtcgcact gcgccgctcc ggctacggca aggaccagcc
    12841 gggccgcttc cgcgggatcg ccgtcatgga gggctacggc acctacatgg cacaggtggc
    12901 cgaaatctcg ctcgaggacg gcaaggtgaa ggtgcatcgc atcgtctgcg cggtcgactg
    12961 cggccgggcg gtgaacccca cgatcgtcgt cgcgcaggtc gagggcgcga tcctgttcgg
    13021 cctgtcggcc acgctgtggg gcgagatcaa cgtccagggc ggccgcgttc agcagagcaa
    13081 tttccacgac tgccgcgtcg tgcgcatgaa cgaggtgccg gtcatcgaaa cccatctgat
    13141 cgagagcgac gagccacccg gtggcatcgg tgaacccgcc acggcactgg tcgcgccggc
    13201 catctgcaac gccatccatg cggcgaccgg ccggcgcctg cgctcccttc ccctcgcgag
    13261 gcacaacctg gcatgagcag caacagcgac ctgcagaaac ggcgcgaggc ggcgataccg
    13321 cgcggcgtcg gcaacagcac cgcgatctac gcggattacg cccgcaacgc cgagatccgg
    13381 gacgtcgagg gccgccgcta catcgacttc gcaagcggca tcgccgtgct gaacaccggg
    13441 catcgccatc cggcggtcga ggccgcgctc gaggcgcaga tcgggcgcct cacccacgcc
    13501 tgcttccagg tcacgcctta cgagagctat gtggcgctcg ccgaggcgct caatgcgctc
    13561 gcgccgggcg cgacgccgaa gaagacgatc ttcctcacca ctggcgccga ggcggtcgag
    13621 aacgcgatca agatcgcgcg ctaccacacg cgccgctcgg cggtgatcgc cttcgccggc
    13681 gggtttcacg gccgcaccct cgcctgcagc gcactcaccg gcaaggtgat gccctacaag
    13741 gcgggattcg ggccgatgat gcccgaggtt ttccacgcgc cctttcccat cgcgtaccac
    13801 ggcgtcagtg cggacgattc gctcgctgcg ctcgagcaac tgttcaaggc cgacgtcgac
    13861 ccggcgcgcg tcgccgcgat catcatcgag ccggtgctcg gcgaaggtgg cttctacgtg
    13921 gcgccggccg acttcctgcg acgcctgcgc gcgttgtgcg accggcacgg catcctgctc
    13981 atcgccgacg agatccagac cggctttgtg cgcaccggcc gcatgttcgc gatcgagcat
    14041 gccggtatcg agccggacct gatgacggtg gccaagagcc tcgccggtgg cgtgccgctg
    14101 tcggccgtga tcggcaaggc cgaaatcatg gatgcgcccg caccgggcgg cctcgggggc
    14161 acttacgcgg gctcaccgct cggctgtgcc gcagggctcg cggtcctcga ggtgatcgag
    14221 cgcgagcagc tcgctgcgcg cgccgtggtg ctcggcgagc ggatagtgac gcgcctgaag
    14281 gcgctgcagg cgcgccatgc ctgcatcggc gaggtgcgcg gtctcggtgc gatggtggcg
    14341 atggagctcg tcaggaaccg caaggcggac gcccccgatg ctgagctgac gaaacagctc
    14401 gtccaggccg cggccaggcg tgggctcgtg atcctctcgt gcgggatcca ctccaacgtg
    14461 atccgcttcc tcgcgccgct gacgatcgag gacgcgctgc tcgaggaggg gctcgaaatc
    14521 cttgaggccg cgctcgacga ggtgacggcc ccggccgcgg gcctcgaacg ccaggcccag
    14581 gggtcctagg cgcgccccgc gttggaatcg gtcggcgtgt ggaagtactc gctctcgctc
    14641 gcgagttcct ccatcagtcg cttcaattcg gccatgcgac cggtcgcagt gctgatcggc
    14701 atacagtcct ggcagcgcgg gtcgccgcag ggctgtcgcg aatagagcca gtactgcacc
    14761 gcgccggcga gcaggccgtc ggcgatgtcc cacagatccg cgtccttgtc tttgtcggcg
    14821 atcttgttac ctagatcgac cgccttgctg aaggccgcgt caaaactgtt gccgagttcg
    14881 ctcatggagg cattgtaagc taccggggca ctcttcgccg acccgaccgc tgcgcgggct
    14941 tcccggacgg gaatacgcgc tcggccgtca gcacccgcgg ccgtgccggt tcgctgtgcg
    15001 cccgtgggct gggcgcattc gccggctcag aacatcctca tcccgatcca ccacgcgatc
    15061 gcggcgaaga acgccgaggc gggaatcgtg accagccatg cgagtacgat gttgccggcg
    15121 atgccccagc gcacggcgga cgcgcgctgg gccgtaccga cgccgatgat ggccccggtg
    15181 atggtgtggg tcgtcgagac cggaatgccg aatgacgtgg cgccgaacag ggtcagcgcg
    15241 ccggccgatt cggccgcaaa gccgccgacc ggccgcagct tcgtgatgcg ctgccccatc
    15301 gtcttcacga tccgccagcc cccgaacaaa gtaccgatcg agatcgcggc atagcagctg
    15361 atgatcaccc ataccggcag gtggtcgcgc gtcgtctcgc cggccgcgat cagcagcagc
    15421 cagatgatgc ccatggtctt ctgggcgtca ttgctgccgt ggccgatgct gtagagcgcc
    15481 gccgacacga gttgcaggcg cctgaacacc cggtcgaccc ggtgcggcga gaaccgcagg
    15541 cagatccatg agaccgccac catccacaat gcgccgagca cgaggccgag catcggcgcc
    15601 acgacgatga acgccgacac cttgatgatg cccggcgcga gcagcggcgc ggtgccggcc
    15661 ttcgccatcg ccgcaccgat catgccgccg atcagggcgt gcgaggaact cgacgggatt
    15721 ccgtagtacc aggtgatcac gttccacgtg atcgcaccga cgagcgcgcc gaagatcacg
    15781 tgatagtcga cgaagtcggg atcgatgatg cccttgccga ccgtcgtcgc gactttcagg
    15841 tggaagatgc cgatggcgac gaaattgaac atcgcggccc agacgaccgc ctggtacggc
    15901 ttcagcacac cggtcgatac caccgtcgcg atcgagttcg cggcatcgtg gaagccgttc
    15961 atgaagtcga acagcaacgc gagcacgacg agcatcgcga gggtcgtcag gacgatttcg
    16021 ccgctcatcg tcagccgttc tcgagcacga cgccttcgat cacgttcgcg acgtcctggc
    16081 acttgtcggt cgtcgcctcg agcagttcgt acacggcctt cagtttgatc agctggcgat
    16141 cgtctcgctc gtcgcggaac agcttcgaga ccgccccgcg catcacgcgg tcggcatcgg
    16201 attcgagtcc gtcgatgtcg aggctgatct tcatgatctc cttggcgttc ttcatcgagg
    16261 agagcagccc gatcgccgcc tcgacccgct cacagcagat ctgcacgaga ttggcgagat
    16321 ggatcgcctc ggcagtgagg tgctggatgt cgtagagcac cagcgtctcc gcggtgtcct
    16381 ggatcaggtc caggatgtcg tccatgcggg aaatcagcct gtggatcacg tcccggtcga
    16441 acggcgtgac gaaggaccgg tgcagcagct cgaccgcctc gcgcgtgacg cgatcggcat
    16501 cgtgttcgat cgtttcgatg cgggaaatgc gggcggcccg gccatccaca tcgccgtagt
    16561 cctgcagcag ctcgaagagc tccctgccgc ccttgacgat cagtgcggca tgttcgttga
    16621 acagcgtgaa gaactggccc tcgcgaggca tcagggctga aaacaggttc atctcgggct
    16681 ccggtcgcgc aggggcggtc cgttcgtggg gcttttgtgc ggtgattatt acacaggccg
    16741 gacggcccgc gccgcgcgcg ctatcttgta cggatgaccc gtatcgcgct actcgatgcc
    16801 cacctcgcct tcggcctgct gccgctgctc gacggcgccg accttcatgt cacggccggc
    16861 gagcgcatcg gcctcatcgg gcgcaatggc acgggcaagt cctcgctgct ggctgtcctc
    16921 gcgggacgcc aggacctcga cgcaggcgaa gtgcagcgtc gcgacggcct ctccgtcgcg
    16981 ctcgtcgagc aggagcccga tctgccggcg gcacccacgc tcggcgcgag cctcgcgctg
    17041 cgcggcggcg ccgaacgcct cgacgaggcc gcgcgcgccg cgctcacggt gcgcgtcacg
    17101 gcgcagctcg accgcttcgg cctcgcgccg gggcagccgt cgagcggcct gtcgggcggg
    17161 gaacgcaagc gcgccgcact cgccctcgcc ttcgcgctcg agcccggcct cctgctgctg
    17221 gatgagccga cgaaccatct cgacatcgcc ggcatcgagc agctcgaggc actggcggcc
    17281 ggcgctgcga cgctcgtcgt cgtgacgcac gatcgccgct tcctcgatcg cgtcgcgact
    17341 cgcatcgtcg aactcgatcg cggtgtactg cgctcatatc ccggcaattt tgcggcctac
    17401 gagcgcgcga agacacagga gctcgccgcc gaggcggtcg cgaaccgccg cttcgacaaa
    17461 ttctggaaac aggaggaagc ctggatcagg cagggcgtcg aggcgcgccg cacgcgcaac
    17521 gaggggcgcg tgcggcggct cgagatgctg cgcctcgagc gcagcaggcg ccgcgaacgc
    17581 acgggcgacg tgcgcttcac gctcgctgcg ggcgatcgtt caggccggct cgtcatcgag
    17641 ctcgaccgcg tgagcgtcag cttcggcgcg cggccgatcg tgcgggacct gtcgatgcgc
    17701 atcatgcgcg gcgatcgcat cggcctgatc gggcccaacg gcgccggcaa gacgacgcta
    17761 ctgcgcctca tgctgggcga actcgagccg gactcgggta ccgtgcgcac cggcagcaac
    17821 ctgcgggtcg cctacttcga ccagctgcgc tcgcagctcg atcccgagcg cacggtcgcc
    17881 gagaccatca gtcccggctc cgactgggtc gaggtgggcg gggtgcgcaa gcacgtcacg
    17941 acctacctcg ccgaattcct gttcccgtcg cgccgggcgc gctccccggt gcgcatgctc
    18001 tccggcggcg agcgccaccg cctgctgctc gcccgcctgt tcgcgcagcc cgccaacctg
    18061 ctggtgctcg acgagccgac caacgacctc gacatggaat ccctcgagct gctggaggaa
    18121 acgctgcagg gctacggcgg cacgctgctg ctcgtgagcc atgaccggga gttcctcgac
    18181 aatgtggtga cccagaccct cgtcgccgaa ggcgacgggc gctggcgcga gtacgtcggc
    18241 gggtacagcg actggcttgc gcagcgcggc gtggcgccgc gcgagggcgc aaccagcgcc
    18301 gcgccggtgc gcgatcgcac gcgcgtgcgg cagcgcttcg gctatcgcga acagaaggag
    18361 ctcgatgcgc tgccggcggc aatcgagtcg ctcgaggcgg agaaatccgc gctcaccgcc
    18421 gcgatgtgcg cgccggacta ccatcgcgcc ggccccgcac gcgcccgcga cgatgcggca
    18481 cggctcgccg cgatcgacca cgacatcgag gcggccatgg cgcgctggga ggagctcgag
    18541 gcgcgccgcg cgcagtaggc aaaaaaaacg ccccggcgga ttgctccgcc ggggcgccgg
    18601 taccaagccg gtcagcgctt ctcgaccgga accacgtcgg cttcgaggcc cacgttggcg
    18661 tcgcggtaga agaacagcag gccttcctgc gacggcgaag ccgtctcacc accctccggt
    18721 gccgtcaccg tcagcatcgt gagactgagc ggcgccacgc ccgcggccgg cggtgccgac
    18781 acatcgaagc gcggcagacc cggtgtgaac accttgccgt cgatgtagtc ggtcaggttg
    18841 cccgtgaagt agttgtcgaa cgccagggcc gagatgacca tctgcgaact cgggctggcg
    18901 cccatgaccg acagcggcgc ggtcaggatc acgttgcccg actcgagatc ggtgtcggcg
    18961 tagaaatacg ccgcggccgt gccggtcctg aggttgtaca cgtacaccgc ggtctgtccg
    19021 gtggcggcaa agcccgtcag ttcggccgtg tagagcacga aatcgtcgac gccgtcggcg
    19081 tcgacatcga gatacacgtc gaactcggcc ggatagctcg ggtgggcgcg ggtgccgtag
    19141 gtgctgaccg cgacctggag gccgaactgg ccaccgccga tgcccacgag gcgcgcaccg
    19201 agcgcctgca tgtcgaccac ggcgaagttc tcgccggggc ccggcagctg cgagcgcgga
    19261 atgcgggcac tcgtgccgag cagtgcgaac ggctcgacca cacctgcctt ggcgggcgcc
    19321 agattgagca gcggcagctg cgccgaagtg tggtcctgcg gaatgacgac cccggcgccg
    19381 atcgcctgca ccagtgccgc cttcttcggc aatacgtgcc acggcacgtg cacgctgtcg
    19441 gcaccgttct tcagggtcag gtagccgtcg tactccgcgg cctgcagcag cgcgccattg
    19501 ccgccctgcg agccgccggc tccggtgtac ggccagtcgc cgagcttcgc cggattgatc
    19561 gacagggtca ccctgaaggt cgcggtgccg ttggccggaa ccgtgacggt cgacggggcc
    19621 gatatcgtga ccgcgccgct gctcgcgtcg tcagcataac ggaacgactt ctgcaccgaa
    19681 tagcggcgcg tcttgttcga gaaattgcgt accttgacgg tgcgccggag cactttcggg
    19741 taactgacgc tgtgatagcc gaacgacagc gcgggcgtct tggtcgccgc gtcccacatc
    19801 gccgtcgtgg ccgcgagcgc cttgttgaca cgcacctcgc caccgccgat gcgggtgatc
    19861 ggcacgagga tcttgtcctc accggacggc gcccgctgat acacctccgg atcggccgtg
    19921 ttggcgagca gcgcgcgcac cagggtcggc gtcagcgagg ggttcttcga aagcagcagc
    19981 gcggccgcgc ccgacaccat cggcgcggcg cccgacgtgc cgccgaaggc ttcctcgccg
    20041 ttgccgctgc cggcgatcgc cgacaccgac gcgcccggcg cgccgatttc gggcttgatc
    20101 gcagcgaagc tcgtcgacgg tccacgcgag gaggtgccga cgatccagcc ctggcgcgac
    20161 tgcgcattgg cctccgaaat cgtggccgac accacattcc cggcgttggt cgccgcgcgg
    20221 atcgaattgg cgaccgcctg cgtgaccacg agcgtgggca cgcactggcc gctgcactcg
    20281 ccgccattcg agaacgacac ggcgtcgccc ggggcgacga ggccgaggat cacggcggtc
    20341 gcgccggcag ccgtcgcatt ggcgaccttg aggctgatcg cgcaggtgcc gcgattgatc
    20401 aacgcgatat tgcccgccac cgtggccggt accgagcccg catcgcaggc caggccggca
    20461 accggcacga cctcgccgct ggtggtgccc gcaatggg
//
LOCUS       DAOQVY010000037        20132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_3126_length_20132_cov_5.425213, whole genome shotgun sequence.
ACCESSION   DAOQVY010000037 DAOQVY010000000
VERSION     DAOQVY010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 20132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20132
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3126_length_20132_cov_5.425213"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /gene="lepB"
                     /locus_tag="PKN91_05245"
     CDS             <1..674
                     /gene="lepB"
                     /locus_tag="PKN91_05245"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNS27515.1"
                     /translation="FEPFRIPSDSMMPTLLDGDFIVVSKFAYGLRLPVTNSRVVDLGS
                     PRRGDVVVFRYPPDPRINYIKRVVGLPGDRVRIEADRVVVNGEPVSFEPLGLYSDGCY
                     VDMQLAEEKLGEHAHRVLYCNTPRDFSVPVLPTCDRRMSRNYVCTPEALALNSDHGDV
                     SDIDVPPGKYLMIGDNRDNSADGRYFGFVPEENLVGRATRIWFNWDPHRAGGPKWDRI
                     GARIE"
     gene            693..1058
                     /locus_tag="PKN91_05250"
     CDS             693..1058
                     /locus_tag="PKN91_05250"
                     /inference="COORDINATES: protein motif:HMM:NF027463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HNS27516.1"
                     /translation="MRARQTGITFIGWLFILVPIALVGYSAIRLTPVMLNYMKVAQAL
                     DRTATQLASDDTLTARSIRVALEKQFDIDSINNPNVREIEITKDGAAWIMRARYDGYA
                     PLFANASILIEFDKVSTID"
     gene            1061..1747
                     /gene="rnc"
                     /locus_tag="PKN91_05255"
     CDS             1061..1747
                     /gene="rnc"
                     /locus_tag="PKN91_05255"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNS27517.1"
                     /translation="MHDEWPLAWVRANLGYVPRDTELFAIALTHRSASGNNNERLEFL
                     GDAVLNLLVADRLYAEFPLASEGDLSRLRARLVSEDPLAGIAGRIGLGEVLRLGSGEL
                     KTGGFRRQSILADAFEAVVGALYVDAGLDAARRIVAPLFDALVRELPDPDELKDAKTR
                     LQEHLQGQGLPLPRYSLAGVEGEPHAQTFHVRCEAPTLGAPSEGSGSSRRRAEQQAAE
                     RMLARLEDGG"
     gene            1749..2654
                     /gene="era"
                     /locus_tag="PKN91_05260"
     CDS             1749..2654
                     /gene="era"
                     /locus_tag="PKN91_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNS27518.1"
                     /translation="MTTTTPEAQFRAGFVALVGRPNVGKSTLLNALVGEKLSIVTPRP
                     QTTRHRILGLVNLPHAQIAFVDTPGLHRGARRALNRAMNRTAATALADADVVLLVVEA
                     LRWTDEDQLALERVKTGGRPCVAVVNKMDRVHPRERALPYVAELAARHAFEEIVPCSA
                     RDADNLERLREVIVARLPQSPPLFPPEQLTDRDSAFRIAETIREKLTLELNQELPYGI
                     AVAIERMTEADGQLVVSAVIWVDREGQKPIVIGEGGERLKRIGRAARIELNRLLGRRL
                     HLELWVKVREDWADNAQALQALGLE"
     gene            2651..3394
                     /gene="recO"
                     /locus_tag="PKN91_05265"
     CDS             2651..3394
                     /gene="recO"
                     /locus_tag="PKN91_05265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNS27519.1"
                     /translation="MTRTLRKAELARAYVLHHRPYRDTSRIVELFSREHGRLALFARG
                     TRGPKSRSASLLQPFVPLLVSWRGHGDAAQLTAVEPAGAAGPGPSALPPGVVMAAYYL
                     NELILKLTTRDDPQVELFDAYAAALAALAGSAAPELPLRSFERRLLEVLGYGINLSTE
                     MDSGRAIDPDGRYVYRPGQGVVKCVADVPGAVAGSSLLRLATDSLSTPGELEDARRVL
                     RAALDYCLEGRELATRAVARAVADKERNR"
     gene            3391..4125
                     /gene="pdxJ"
                     /locus_tag="PKN91_05270"
     CDS             3391..4125
                     /gene="pdxJ"
                     /locus_tag="PKN91_05270"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNS27520.1"
                     /translation="MSATAIALGVNIDHVATLRQARRARYPDPVHAALAAEQAGADNI
                     TLHLREDRRHIQDRDVRALRELLQTRMNLECAVTDAMLAIARDVRPADCCLVPERREE
                     VTTEGGLDVAGQLRRVSRACEDLAGAGVRVALFIDADERQIEAAAQAGAPVIELHTGA
                     YANAEGSALARELDKLRAGARRAAALGLEVHAGHGLHYHNVAPVAAITEIVELNIGHA
                     IIARSIFDGLARAVGDMRALMQAARR"
     gene            4122..4517
                     /gene="acpS"
                     /locus_tag="PKN91_05275"
     CDS             4122..4517
                     /gene="acpS"
                     /locus_tag="PKN91_05275"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNS27521.1"
                     /translation="MIFGIGVDVLEAARVQKVYERHGEHFVERLLMPAERAQLGLTKR
                     KERFLAMRFAAKEAIVKAMGTGFAHGVWIRDVGIVQNAWGRPEVVYSLRGEAVRRALG
                     VGDGHVTLTDEAGLIVAVAVLERAVEGGR"
     gene            4514..5296
                     /locus_tag="PKN91_05280"
     CDS             4514..5296
                     /locus_tag="PKN91_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNS27522.1"
                     /translation="MNVLVFDIETVPDVELGRRAYGLEGLADAEVAKAMFTRQRQATG
                     HDFLPHEQHRVVAISCALRTRDTLRVWSLGDRDSNEADIVGRFYEGIERFTPDLVSWN
                     GGGFDLPVLNYRALAAGIQAPTYWETGDGNTSFRYNNYLSRYHWRHLDLMDVLSGMQA
                     RARASLEDMAHLLGLPGKLGFSGAMVWDAYLDGEIDAIRRYCETDVLNTYLVYLRFEL
                     LRGHFSREQYAAEVEQLRALLEASGEPHHAEFLAAWTTSKRA"
     gene            5272..6573
                     /gene="rlmD"
                     /locus_tag="PKN91_05285"
     CDS             5272..6573
                     /gene="rlmD"
                     /locus_tag="PKN91_05285"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNS27523.1"
                     /translation="MDDIEAGVVTALNHDGDGIVKSGKTAFVPGVLPGETVRFRRTRR
                     HRQHDEAQLVEVLVPAADRVEPQCPHFGTCGGCALQHLAPAAQLAAKAAELADSLARI
                     GRVEPRTWLAPLEGPVWNYRRRARLSARYVHKKGRSLVGFRERSAPYVAEIDSCRVLA
                     PPFDQLVSPLSALLTGLTIRERVPQVEIASGERANAVILRVLSTPSADDLAAMRAFGA
                     AHDVTIYLQSGGLDTVAPLDGVPAQLDYELPDFGLRLAFEPVDFVQVNAAINRALVSR
                     AVEWLDPGPADRVLDLFCGLGNFTLALARRAGEVVGVEGDAGLIGRARANAVGNGIDN
                     ATFHVANLAAEDVDQSRWWAGGFTQVLLDPPRAGAREVLPAVARLAPRRIVYVSCHPG
                     TLARDLGELVHEHGYLLQAAGVLDMFPHTTHVESMAVLARA"
     gene            6614..7666
                     /gene="nagZ"
                     /locus_tag="PKN91_05290"
     CDS             6614..7666
                     /gene="nagZ"
                     /locus_tag="PKN91_05290"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNS27524.1"
                     /translation="MTLGPLMVDLKGTELEPLEREMLAHPLVGSVILFARNFVDPEQL
                     GYLTAAIHAVRQPALVIAVDQEGGRVQRFRQGFSRLPPLRRIGQEYDLDQRRGLELAR
                     SLGWLMAAELRACGVDISFAPCVDLDYGVSEVIGDRAFHARASAVADLAVAYMHGMRD
                     AGMGATAKHFPGHGAVVADSHHALPVDRRDLVDMDDDLAPYRRLIANGLPGVMVAHVA
                     FPAVDDPPASLSARWIRQLLREELGFSGVVFSDDLSMRGAAAGGDIVTRSRAALAAGC
                     DVLAVCNDQTAVATLLDTLHVEPDPAMHLRLARLRGREGLSHSALLASDAWRQSQRAL
                     AHCAEAPALELEAGRA"
     gene            7663..8592
                     /locus_tag="PKN91_05295"
     CDS             7663..8592
                     /locus_tag="PKN91_05295"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNS27525.1"
                     /translation="MKDWSAGFLRSVLDAAPEGIVICEAGEDQPVVYVNAAFEYLTGH
                     DAADLVGRNLRHLQGTDREQEARYRMQQAIERGDSCQVMLRNYRKSGGVYWVDIHMQP
                     VRDASGRVTHFIGYHRDASDRPRTAVRALAGMPSWMREDRLSGLATRPYFEELLKRDW
                     ALAQREMRQLGLAIFDIDSLGAYNDTYGRAGGDACVRRTAHLIAASFRRGSDLLGRWD
                     GGGVIVLSPGHDQEKLVAYARLIAQRVRDQQVHHPRSAAKFVTVSVGVACCMPMRDQP
                     VSLLVGAAEAALARAKTAGRNQVAVAEKGDYVA"
     gene            complement(8608..9147)
                     /locus_tag="PKN91_05300"
     CDS             complement(8608..9147)
                     /locus_tag="PKN91_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27526.1"
                     /translation="MRLPRPLLALLAALVAMPAMPQASDPVYHIEIIVFRTGGSSGNA
                     GGLGLGIDSQSSGDDSGGGGIARLIGPLAASSLQLGDIAARLRASGAYRPIAHAGWAQ
                     TPSRWGSRSGFSLGRVGVNAEGLAGTAYLERGQYLHLGFSINLGGATINEIRRIRLGE
                     KNYFDNPEFGVIAMVTAAR"
     gene            complement(9180..12635)
                     /gene="mfd"
                     /locus_tag="PKN91_05305"
     CDS             complement(9180..12635)
                     /gene="mfd"
                     /locus_tag="PKN91_05305"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007966.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNS27527.1"
                     /translation="MNILEPLIPDGSCRHVRWHQLYGGAPSLALAEAAARARGLLVVV
                     CASARELERRAAEIGFYSDGRLPILALLDWEVLPYDRFSPHPDITSERLATLADLRDA
                     RHGVLLVAADTLLGRLPPAQYVIGRSFRLARGERLALVPLRTRLVESGYAAVSQVTGP
                     GEFALRGSLLDVFPMGSSHPLRIDLFDDEIEAIRRFDPDTQRSLDALESVRLLPARET
                     PLDPDAVRAFRRRYRTRFEGDPTRSEIYRGVSEGIAPPGIEFYLPLFHDTTATLTDYL
                     PPGALLATGAGLEADLERAWQEIEARHEAHRHDIERPLLAPAELYVPPNELLAQLDAL
                     PRITIDPFKAEIGGESDPGVRNFPTAAPREMRLDVRADQPLAPLDAFMAGYQGRVLIA
                     ADSAGRREVLVEMLRGHHTPVTPVDGWGAFVRGEARFALTVAPEIDGLTLIDPPIALL
                     AESQLFGARARQERRRRRAASDPQAILRDLRDLSPGAPVVHEEYGVGRYVGLTAMDVA
                     DQPGEFLVLEYQDGDRVYVPVHSLHLVSRYTGSTPETAPLHKLGTDQWARARKKAAAQ
                     VRDVAAELLDLYARRQARRGLALPLRELEYQSFANAFPFEETPEQADAIAKVIEDLAS
                     DRPMDRIVCGDVGFGKTEVAMRAAFVAVQAGRQVVVLVPTTLLAEQHVANFRDRFADW
                     PVRIEGLSRFRSAKESNAVIDGLAAGTVDIVVATHRLLHANVRLKDLGLIIIDEEHRF
                     GVRDKERLKTLRAEVHVLTLTATPIPRTLNMAIGGLRDLSLITTPPAERLAIKTFVTE
                     WQDTALREAILRELRRGGQVYFVHNEVRDIDKIAASIAQLVPEADVRIGHGQMRERDL
                     EQLMVDFYHRRFNVLVCTTIIESGIDVPTANTIIIDRADRMGLAQLHQLRGRVGRSHH
                     RAYAYLLAPPRKALSQDAARRLEAIESMEELGAGFVLATHDLEIRGAGELLGESQSGE
                     LTEVGLSLFLDMLEHAVKALREGREPQLDRPLGAPTEIELRLPALLPESYVGDVHVRL
                     ALYKRIAAAADEPAIDDLTAELVDRFGPLPGPAIALLRLARLKLAARALGVRKLDLGP
                     QGGYVLFEEENAVDPRAVIRLVQDRSREYRLDGPLKLRVARDLDEEDERFDFAATLLS
                     KLRAA"
     gene            complement(12708..12989)
                     /locus_tag="PKN91_05310"
     CDS             complement(12708..12989)
                     /locus_tag="PKN91_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27528.1"
                     /translation="MSSRAVPWLVAGLLFGGVAAAETIVVDGQVAVRQTSVERPSRGS
                     TMSTVEQRFGAPNAKHAAVGQPPITRWDYAGFSVFFEHDRVIHAVVVGG"
     gene            13101..14345
                     /locus_tag="PKN91_05315"
     CDS             13101..14345
                     /locus_tag="PKN91_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HNS27529.1"
                     /translation="MFHPLSLFVGLRYVRARSHKFFVSFITWVSVVGVCVGVAALIVI
                     LSVMNGFEGELRDRLLALSAHARLLPVGEEPADLEALAAAASEVPGVTGAAPYVEVQA
                     LALRSPEMLPVVLRGIDPAREGAVTDIARAVTVGALADLVPGTNRVILGSVVAQRLTA
                     GPGDTITLLVPSIGAGGAPEPRLREFAVAGLFEVGLQDHDGTLVLAAFDDVRAVLPRA
                     GNPGSVHLRFDDALAAPARAAALRTAFAGQAEVRDWTQDHANYFRAIRIEKTMMSLIL
                     LLIVAVAAFNIVAMLVMVVTDKRTDIAILRTLGTAPRRVMGVFATQGLVIGWFGVIAG
                     IALGVLIARNVTPIVAFLERAFGFQIMDADVYYITRIPSVLEWHNVAWIGGAAFLLTA
                     LATVYPALRAARVAPAEALRYE"
     gene            14349..15596
                     /locus_tag="PKN91_05320"
     CDS             14349..15596
                     /locus_tag="PKN91_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HNS27530.1"
                     /translation="MTRFYEWWIGTRYLSSTHRRGFVSIVALISVIGLMLGVAVLIVV
                     LSVMNGFERELRTRILAVTAHATLMGLEGTLDDWREARAVALGTEGVRAAVPYVEAQS
                     MLAHGSVITGAQVRGIDPDEEGKADGLESRMTSGSLGALFPGSWNIVLGDALAAELGA
                     QAGDTLVLIAPEGSATPTGVMPRMRRFTVSGIFSSGMYEFDRGLALVNLQDAARLYRT
                     GDGVTGLRLELDDAFAAPRIVREVALGLGGGFYVSDWTRNHANFFRSIEITKSLMFVI
                     LLMIVGVAAFNIVATLVMIVKEKQTDIAILRTLGAGPRNVLGAFTVQGVLIGLAGTAL
                     GAGLGMLVARNLESLVRSLERALGTQFLDARVYYMSDLPAYVEWQDVLQVCGVAFALC
                     ALATLYPAWRAARTAPAEALRHE"
     gene            15589..16269
                     /locus_tag="PKN91_05325"
     CDS             15589..16269
                     /locus_tag="PKN91_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS27531.1"
                     /translation="MSEAVLDARGLRREFRQGPVLLEVLRGTDLVVGVGERLAIIGAS
                     GSGKTTLLQVIGGLDRPTAGTVLVDGRDIHAVGERERGELRNRAIGFVYQFHHLLPEF
                     SALENVAIPLLVRRERVAVARERAAALLDRVGLGARLTHRPHQLSGGERQRAAVARAL
                     VTEPRLVLADEPTGNLDGANAEQVFQLMLELNRERGTSLVVVTHDPRLAARMDRVLRL
                     EDGVLVPA"
     gene            complement(16306..16812)
                     /locus_tag="PKN91_05330"
     CDS             complement(16306..16812)
                     /locus_tag="PKN91_05330"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNS27532.1"
                     /translation="MIRRFTPSPEQIRSHWYLRPFGTRIADPALWSLQRRGVTAAFGA
                     GIAIGFVPLPVHLILAAIVAITARINAPVLVATVFLVNPFTFVPVYYFAYRVGALMLG
                     HIPQPFRFELSWTWLQYGLGPLWKPFLVGCLACAVVGGLLGWALLELFWRIHVRQKYR
                     ARPARTRR"
     gene            16857..18938
                     /locus_tag="PKN91_05335"
     CDS             16857..18938
                     /locus_tag="PKN91_05335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNS27533.1"
                     /translation="MTLAAAGAFLAGALLALAVPTPYPPHAALAVLLAALGAALAWRA
                     GWPLATVLAAWLLAGLVCAAVTTSLRLAERWPQSRSGERVLVTARVASIPVDEEYGTA
                     FDALLTLDPRAGGQRLRARIVWVHPQPRPQVGERWQLAVRLRAPRAALNPNGPDMERV
                     WFRDRVGALGTVLPRSPLNIRLDPGHAPLDRLRARIMRRIAATVVDRDAAALLGALAV
                     GVTGDMSREQWRVFNATGTTHLVAISGMHVTLFAVIAMAGARRVWRVAGRWCTRLRRE
                     SFAAALGIVTAAGYSLLAGFSVPTQRTLLMLSAWLIARHLARAHGALHAFAVALVAVL
                     LLDPLAPLASGFWLSFGAVAAILLATGARLGRAHWLHEAVRVQCAVTIALVPVTLAAF
                     GSVSLASLVVNAAAIPLFTLLLVPLVLAATAAMALWPALATLLLQLAAWLHAQLWPWL
                     EAAADWPHALVQLAPPAWAYLLVVPAAGMAVLPWPRGLRATAAAAILPLGFARALVPA
                     ADEITVAVFDVGRGQAVAIRTADGVLVHGTGDSFGTEGRRMARVVVPWLRTQRVARVD
                     DLVLPHLYRDYAVGAAELDITLGIDRILVARPWPGGPQRAVTCDAHAGWTRAAAHFAL
                     TATCDMTVTVGANRLEVTGKEVFLDTGAGRQHLDSEVRGAIILTLGKAAGAVRRRDAR
                     DGYPWPWRAPV"
     gene            18974..19603
                     /locus_tag="PKN91_05340"
     CDS             18974..19603
                     /locus_tag="PKN91_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNS27534.1"
                     /translation="MWEIVRAGGPLMWPIILCSIGAAAIILERFWTLQDKRVIPPDLT
                     RKVSQLIEGNQINDKVIAALEQNSPLGRILAAGLANRHRPREIMVERLQDTGRHVIYE
                     LERFLNSLNMIAGVSPLLGLLGTVTGIIKAFNAIQAGGMGDPRMLSGGIAEALITTAA
                     GLSVAIPSLIGFRLLRGKVDRIVIQMEKDALRLADEVDAAAGRGRAGAA"
     gene            19600..20031
                     /locus_tag="PKN91_05345"
     CDS             19600..20031
                     /locus_tag="PKN91_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNS27535.1"
                     /translation="MNFKPRRTDEPELNMTPLIDVVLLLVVFFMLSSTFVQEGRLRVR
                     LPEARDVPVTRPAQEPIVVSVTEGGGYRVNERDLVNAGADTLRAALLAVAGEERGIPV
                     TLRADARASHQAVVTAMDVLGKLGFAEINIATVNDADAAGP"
BASE COUNT         2913 a         6867 c         7193 g         3159 t
ORIGIN      
        1 tgttcgagcc gtttcgcatt ccgtccgact cgatgatgcc gacactgctg gacggcgatt
       61 tcatcgtcgt cagcaagttc gcctacgggc tgcgtctgcc ggtgaccaat tcgcgcgtcg
      121 tcgacctcgg cagcccccgg cggggcgacg tggtcgtgtt ccgctatccg cccgatccgc
      181 gcatcaacta catcaagcgc gtggtcggcc tgccgggcga ccgggtgcgc atcgaggccg
      241 accgggtggt cgtgaacggc gaaccggtgt cgttcgagcc gctcggcctg tacagcgacg
      301 gctgctacgt cgacatgcag ctcgccgagg agaagctcgg cgagcacgcg caccgggtgc
      361 tctactgcaa cacgccgcgc gacttttcgg tgccggtgct gccgacctgc gaccggcgca
      421 tgagccgcaa ttatgtctgc acgccggagg cactcgccct gaattccgac catggcgacg
      481 tgtcggatat cgacgtgccg ccgggcaagt acctgatgat cggcgataat cgcgacaaca
      541 gcgccgacgg ccgttacttc ggcttcgtac cggaggagaa tctggtcggc agggcgacgc
      601 gaatctggtt caattgggac ccgcatcgcg cgggcgggcc caaatgggat cgtatcggcg
      661 cgcggatcga atgagacaac gggggagtgg cgatgcgtgc acgtcagaca ggaatcacct
      721 tcatcggctg gctgttcatc ctggtgccga ttgcactggt gggttactcg gcgatccggc
      781 tgacgcccgt gatgctgaat tacatgaagg tcgcgcaggc gctcgatcgc actgccaccc
      841 agctcgcgag cgacgacacg ctgaccgcgc gctcgatccg cgtcgctctc gagaagcagt
      901 tcgacatcga cagcatcaac aacccgaacg tgcgcgagat cgagatcacg aaggacggtg
      961 cggcgtggat catgcgcgcc cgctacgacg gctatgcgcc gctgttcgcg aatgcctcga
     1021 ttctgatcga gttcgacaag gtgtcgacga tcgactgacg atgcacgacg aatggcccct
     1081 cgcatgggtg cgcgcgaacc tcggctatgt gccgcgggac acggaactgt ttgcgattgc
     1141 gctgacgcac cgcagcgcat cgggcaacaa caacgaacgc ctggaattcc tcggcgacgc
     1201 ggtgctcaac ctgctggtcg ccgatcgcct gtatgccgag ttcccgctcg cgagcgaggg
     1261 tgacctgtcg cggctgcgcg cgcgcctggt cagcgaggat ccgctcgccg gcatcgccgg
     1321 ccggatcggc ctcggcgagg tgctgcgcct cgggtccggc gaactgaaga cgggcgggtt
     1381 ccggcgtcag tcgatcctcg ccgatgcctt cgaggcggtg gtcggggcgt tgtacgtcga
     1441 tgccgggctc gacgcggcgc gtcgcatcgt cgcgccgctg ttcgatgcgc tggtgcgcga
     1501 gctgccggat ccggatgagc tgaaggacgc gaagacgcgc ctgcaggagc atctgcaggg
     1561 ccagggactg ccgctgccgc gctacagcct cgccggcgtc gagggcgaac cgcacgcgca
     1621 gaccttccat gtgcgttgcg aggcgccgac gctcggcgcg ccgagcgagg gcagcggctc
     1681 gagccggcgc cgcgccgagc aacaggcggc cgagcgcatg ctggcgcgcc tcgaagacgg
     1741 cggctgacat gaccacgacc accccggaag cgcagttccg tgcgggcttc gtcgcgctgg
     1801 tagggcggcc gaatgtcggc aagtcgacct tgctcaatgc gctggtcggc gagaagcttt
     1861 cgatcgtgac cccgcggccg cagaccacgc ggcatcgcat cctcggcctc gtcaacctgc
     1921 cgcacgcgca gatcgcgttc gtcgacacgc ccggactgca tcgcggtgcg cgccgcgcgc
     1981 tcaaccgcgc gatgaaccgc acggcggcta cggcgctcgc cgatgccgac gtcgtgctgc
     2041 tggtcgtcga agccctgcgc tggaccgacg aggaccagct cgcgctcgag cgcgtgaaga
     2101 cgggtggcag gccgtgcgtc gccgtggtca acaagatgga cagggtgcac ccgcgcgaaa
     2161 gggcgctgcc gtacgtggcg gaactcgcgg cgcgccatgc attcgaggag atcgtgccgt
     2221 gctcggcgcg cgatgcggac aatctcgagc gcctgcgcga ggtgatcgtc gcgcgcctgc
     2281 cgcagtcgcc gccgctgttt ccgccggaac agctcaccga tcgcgacagc gcgttccgca
     2341 tcgccgagac gatccgcgag aagctgacgc tcgagctcaa ccaggagctg ccctatggca
     2401 tcgcggtcgc gatcgagcgg atgaccgagg cggacgggca gctcgttgtc tcggcggtga
     2461 tctgggtcga tcgcgaagga cagaagccga tcgtgatcgg cgagggcggc gagcggctga
     2521 agcgtatcgg gcgcgccgcg cgcatcgaac tcaatcgcct gctcggtcgc aggctgcacc
     2581 tcgaactgtg ggtcaaggtg cgcgaggact gggccgacaa cgcgcaggca cttcaggcgc
     2641 tggggctcga atgacgcgta cgctgcgcaa ggccgaactc gcccgcgcct acgtcctgca
     2701 ccaccggccg tatcgcgata cgagccgcat tgtcgaactg ttcagccgcg agcacgggcg
     2761 cctggcgctg ttcgcccgcg gcacccgcgg cccgaagtcg cgcagcgcct cgctgctgca
     2821 gccgttcgtg ccattgctgg tgtcctggcg gggtcatggc gacgcggcgc aactcacggc
     2881 ggtggagccg gccggtgcgg ccggacccgg cccatccgcg ctgccgccag gggtcgtgat
     2941 ggcggcgtac tatctcaatg agctgatcct gaagctcact acccgcgacg acccgcaggt
     3001 ggagcttttc gacgcctacg ccgcggcgct cgccgcgctc gcgggcagcg cagcgcccga
     3061 actgccgctg cgcagcttcg agcggcgact gctcgaggtg ctgggctacg gcatcaatct
     3121 ctcgacggaa atggacagcg ggcgcgcgat cgacccggac gggcgctatg tctaccggcc
     3181 agggcagggg gtcgtgaagt gcgtcgcgga tgtgcccggg gccgtcgccg gcagttcgct
     3241 gctgcgcctg gcgacggatt ccctgtcgac gcccggtgag ctcgaggatg cgcggcgtgt
     3301 gctgcgcgcg gcactcgact actgtctcga aggacgtgaa ctcgcgacgc gcgcggtggc
     3361 gcgcgcggtg gcggacaagg agcggaaccg atgagcgcga cagcgatcgc actcggcgtg
     3421 aacatcgacc acgtggccac actacgccag gcgcggcgtg cgcgctatcc cgacccggtg
     3481 cacgcggcgc tcgcggccga gcaggccggt gccgacaaca tcacgctgca cctgcgcgag
     3541 gatcggcgcc acatccagga ccgggacgtg cgcgcgttgc gcgaactgct gcagacacgc
     3601 atgaatctcg aatgcgcggt gaccgatgcg atgctggcga tcgcccgcga cgtgcggccc
     3661 gccgactgct gcctcgtgcc cgaacgacgc gaggaagtga ccaccgaagg tggactcgac
     3721 gtcgccggcc agctgcggcg ggtgtcccgg gcctgcgagg atcttgccgg agcgggcgtg
     3781 cgcgtggccc tcttcatcga tgcggatgag cggcagatcg aggcggcggc gcaggccggg
     3841 gcgccggtca tcgaattgca caccggcgcc tacgccaatg ccgagggttc agcgctggcg
     3901 cgcgagctcg acaagctgcg cgccggcgca cgccgggcgg ccgccctcgg gctcgaagtg
     3961 catgcggggc acgggctgca ctaccacaac gtggcgccgg ttgcggcgat caccgagatc
     4021 gtcgagctca atatcggcca cgcgatcatc gcgcgctcga tcttcgatgg cctcgccagg
     4081 gccgtgggcg acatgcgtgc gctgatgcag gcggcgcgcc ggtgatcttc ggcatcggtg
     4141 tcgacgtgct cgaggcggcc cgcgttcaga aggtctacga gcgccacggc gagcacttcg
     4201 tcgagcggct gctgatgccg gccgagcgcg cgcagcttgg gctgaccaag cgcaaggaac
     4261 gcttcctcgc gatgcgcttc gccgcgaagg aggcgatcgt caaggccatg ggcaccggct
     4321 ttgcgcacgg tgtctggatc cgtgacgtcg gcatcgtgca gaacgcgtgg ggccgacccg
     4381 aggttgtcta ctcgctgcgc ggcgaggcgg tgcgtcgcgc gctcggggtc ggcgatggcc
     4441 acgtgaccct gaccgacgag gcgggcctca tcgtcgccgt ggcggtgctc gagcgtgcgg
     4501 tggagggcgg ccggtgaacg tgctcgtgtt cgacatcgag acggtgcccg atgtcgaact
     4561 cgggcggcgc gcctacgggc tcgagggcct tgccgatgcc gaagtggcca aggccatgtt
     4621 cacgcgccag cggcaggcca cgggccacga cttcctgccg catgagcagc atcgcgtcgt
     4681 cgcgatctcc tgcgcgctgc gcacgcgcga tacgctgcgc gtgtggagcc tgggtgaccg
     4741 ggactcgaac gaggccgaca tcgtcgggcg attctacgag ggcatcgagc gtttcacgcc
     4801 ggacctcgtt tcgtggaatg gcggcggttt cgacctgccg gtcctcaact atcgcgcgct
     4861 cgccgccggc atccaggcac cgacctactg ggagaccggc gacggcaata cctcattccg
     4921 ttacaacaac tatctgagcc gctatcactg gcgccatctc gacctgatgg acgtcttgtc
     4981 cggcatgcag gcgcgggcgc gcgcctccct cgaggacatg gcgcacctgc tcggtctgcc
     5041 gggcaagctc ggtttctcgg gtgcgatggt ctgggatgcg tatctcgacg gcgagatcga
     5101 cgcgatccgc cgctattgcg agaccgacgt gctgaacacc tacctcgtct atctgcgctt
     5161 cgaactgctg cgcggccatt tctcgcgcga gcagtacgcg gccgaggtcg agcagctgcg
     5221 cgcgctgctc gaggcctcag gcgagccgca ccacgccgaa ttcctggccg catggacgac
     5281 atcgaagcgg gcgtagtcac cgcgctcaac cacgacggcg acggcatcgt gaagagcggc
     5341 aagacggcgt tcgtgccggg tgtgctgccg ggggaaaccg tgcgctttcg ccgcacacgc
     5401 cgtcaccgcc agcacgatga ggcgcagctc gtcgaagtgc tggtgcccgc cgcggaccgg
     5461 gtcgagccgc aatgcccgca cttcggcacc tgcggcggct gcgccctgca gcatctcgcg
     5521 ccggctgcgc agctcgccgc caaggccgcc gaactcgcgg acagtctcgc gcgcatcggg
     5581 cgcgtcgagc cgcgcacatg gctggctccg ctcgaaggcc cggtctggaa ctaccgccgt
     5641 cgcgcacggc tctcggcgcg ctacgtgcac aagaaggggc gctcgctggt cggctttcgc
     5701 gagcgctcgg cgccctatgt cgccgagatc gactcctgcc gcgtgcttgc gccgccgttc
     5761 gatcagttgg tatcgccgct ctctgcgctg ctcaccgggc tcacgatccg cgaacgcgtg
     5821 ccgcaggtcg aaatcgcctc cggcgagcgt gccaacgcag tgatcctgcg tgtgctgtcg
     5881 acgccgtcgg ccgacgacct cgcggcgatg cgggcattcg gcgccgccca cgatgtcacg
     5941 atctacctgc agtcgggcgg cctcgacacc gtggcgccgc tcgacggcgt gccggcgcaa
     6001 ctcgactatg agctgccgga cttcggtctg cgactcgcgt tcgaaccggt cgatttcgtc
     6061 caggtgaacg ccgcgatcaa ccgcgcgctg gtgtcgcggg cggtggagtg gctggatccg
     6121 ggtcccgcgg accgcgtact cgatctcttc tgcgggctcg gcaatttcac gctggcgctc
     6181 gcccgtcgtg caggcgaggt ggtcggcgtc gagggggatg cgggcctgat cgggcgcgcg
     6241 cgcgccaatg ccgtgggcaa cggcatcgac aatgcgacct tccatgtggc caacctcgcc
     6301 gccgaggacg tcgaccagag ccgctggtgg gcgggtggct tcactcaggt actgctcgat
     6361 ccgccacggg ccggcgcccg cgaagtgctg ccggccgtcg cgcgtcttgc gccacggcgc
     6421 atcgtctacg tgtcctgcca tccgggcacg ctcgcgcgcg atctcggcga actggtgcac
     6481 gagcacggct acctgctgca ggccgcgggc gtgctcgaca tgttcccgca cacgacgcac
     6541 gtcgaatcga tggctgtgct cgcgcgcgca tgaccgtggc gcctgcggcg ccaataccgg
     6601 atacttgtgc gccatgacac tcggcccgct gatggtcgac ctgaagggca cggaactcga
     6661 gccgctcgag cgcgagatgc tcgcccatcc gctcgtcggc agcgtgatcc tcttcgcgcg
     6721 caacttcgtc gatccagagc agctcggcta cctgacggcg gcgatccatg cggtacgcca
     6781 accggcgctg gtgatcgccg tcgaccagga gggcggtcgc gtgcagcgct tccgtcaggg
     6841 cttctcgcgt ctgccgccgc tgcggcgcat cggccaggaa tacgacctcg accagcggcg
     6901 cgggctcgaa ctggcgcgct cactcggctg gctgatggca gccgagttgc gcgcctgcgg
     6961 tgtcgacatc tccttcgcgc cctgcgtcga tctcgactac ggcgtgagcg aggtgatcgg
     7021 agatcgcgcg ttccatgcgc gcgcatcggc cgtcgccgac ctcgccgtgg cctacatgca
     7081 cggcatgcgc gatgccggca tgggggcaac cgccaagcat ttcccgggac acggcgcggt
     7141 ggtcgccgat tcccaccacg cgctgccggt cgatcgtcgc gatctcgtcg acatggacga
     7201 cgacctcgcg ccctatcggc ggctgattgc gaacggactg cccggcgtga tggtcgcaca
     7261 cgtggcgttt ccggccgtcg acgatccgcc ggccagcctg tcggctcgct ggatccgcca
     7321 gctgctgcgc gaggagctgg gattcagcgg ggtcgtgttc agcgacgacc tgtcgatgcg
     7381 gggggctgcg gccggcggcg atatcgtcac gcgctcgcgc gcggcgctgg cggcgggttg
     7441 cgacgtgctc gcggtgtgca acgaccagac cgcggtggcg acactgctcg atacgctgca
     7501 tgtggagccc gacccggcaa tgcacctgcg gctcgcgcga ctgcgcgggc gagaaggatt
     7561 gagtcactcg gcgctgctcg cctccgatgc gtggcgacag agccagcggg cactggcgca
     7621 ctgcgcagag gcaccggcac tggaactgga agcggggcgg gcatgaagga ttggtcggcg
     7681 ggattcctgc gcagcgtgct tgatgcggca cccgaaggca tcgtgatctg cgaagcgggc
     7741 gaagaccagc cggtggtcta cgtgaacgcg gcgttcgaat acctgacggg gcatgatgcg
     7801 gccgatctgg tcgggcgcaa cctccgccac cttcagggta ccgaccgcga acaggaggcc
     7861 cgctaccgaa tgcagcaggc gatcgagcgc ggcgattcgt gccaggtgat gctgcgcaac
     7921 taccgcaaga gcggcggcgt gtactgggtc gacatccaca tgcagccggt gcgcgatgcg
     7981 tccgggcggg tcacccactt catcggctat caccgcgatg cgagcgacag accacgcacc
     8041 gccgtgcgcg cgctggccgg catgccgagc tggatgcgcg aggaccggct ctcggggctc
     8101 gcgacgcgcc cctatttcga ggagctgctg aagcgcgact gggcgctcgc gcagcgcgag
     8161 atgcgccagc tcggcctcgc catcttcgat atcgacagcc tcggtgccta caacgacacc
     8221 tacggtcgcg cgggcgggga tgcctgcgtg cgccgtacgg cgcatctgat cgccgcttcg
     8281 ttccgccgtg gcagtgacct gctcgggcgc tgggacggcg gcggcgtgat cgtgctctcg
     8341 ccgggccacg accaggagaa gctcgtcgcc tacgcccgcc tgatcgcgca gcgcgtgcgc
     8401 gaccagcagg tgcatcaccc gcgctctgcg gcgaagttcg tgaccgtgag cgtgggcgtc
     8461 gcgtgctgca tgccgatgcg cgatcagccg gtgtcgctgc tggtgggcgc ggccgaggcg
     8521 gcactcgcac gcgccaagac ggcgggccgc aatcaggtcg cggtggctga aaagggcgat
     8581 tacgtggcgt gagccgcggc cggcgtatca gcgggcggcg gtcaccatgg cgatgacacc
     8641 gaattcgggg ttgtcgaagt agttcttctc gccgaggcgt atccggcgga tctcgttgat
     8701 ggtggcgcca cccaggttga tcgagaatcc gaggtgcagg tactggccgc gctccaggta
     8761 ggcggtaccg gcaaggcctt cagcgttgac gccgacgcgc ccgaggctga agccgctgcg
     8821 cgatccccag cgactcggcg tctgtgccca acccgcgtgc gcgatcggcc gataggcgcc
     8881 gctcgcgcgc agacgcgccg cgatgtcacc gagctgcagg ctggaagccg ccagcgggcc
     8941 aatgaggcgc gcgatgccgc caccgccgct gtcgtcgccg ctcgactgcg agtcgatgcc
     9001 gaggccgagg ccgcctgcat tgcccgagct gccgcccgtg cggaacacga tgatctcgat
     9061 gtggtagacg gggtcgctcg cctggggcat ggcgggcatc gccaccagtg cggcgagcag
     9121 cgcgagcagg ggtcgtggga ggcgcatgga gagagtgtac cgcgaccgcg tacccgcctt
     9181 cacgccgcgc gcagcttcga cagcagggtg gccgcaaagt cgaagcgctc gtcctcttcg
     9241 tccaggtcgc gcgccacgcg caatttgagc gggccgtcga gccggtattc ccgcgacctg
     9301 tcctgcacga gccggatcac ggcgcgcggg tcgacggcgt tctcctcctc gaacagcacg
     9361 tacccgccct gcgggccgag gtcgagcttg cgcacgccga gcgcgcgcgc cgcgagcttg
     9421 aggcgcgcga ggcgcagcag ggcgatcgcg gggcccggca gcgggccgaa acgatcgacc
     9481 agttcggcgg tgaggtcatc gatcgcgggc tcgtccgcgg ccgcggcgat gcgcttgtag
     9541 agagcgaggc gcacatgcac gtcgccgaca tagctctcgg gcagcagtgc gggcagccgc
     9601 agctcgattt cggtcggcgc gccgagcggc cggtcgagct gcggctcgcg gccttcgcgc
     9661 agcgccttga ccgcatgctc gagcatgtcg aggaacagcg acaggccgac ctcggtcaac
     9721 tcgccgctct gcgattcgcc gagcagctcg ccggcgccgc ggatctcgag gtcgtgggtc
     9781 gcgagcacga agccggcccc gagttcctcc atggactcga tcgcctcgag acgcctcgcc
     9841 gcgtcctgcg acagcgcctt gcggggcggg gcgagcaggt aggcgtacgc gcgatggtgc
     9901 gagcgcccga cacggccgcg caactggtgc agctgggcaa ggcccatgcg gtcggcccgg
     9961 tcgatgatga tcgtgttggc cgtcggcacg tcgatgccgc tctcgatgat cgtcgtgcag
    10021 acgagcacgt tgaagcggcg atggtagaag tcgaccatca actgttcgag gtcacgctcg
    10081 cgcatctggc cgtggccgat ccgcacgtcg gcctcgggca cgagctgcgc gatgctcgca
    10141 gcgatcttgt cgatgtcgcg cacctcgttg tgcacgaagt agacctggcc gccgcgccgc
    10201 agctcgcgca ggatcgcctc gcgcagcgcc gtgtcctgcc attcggtcac gaaggtcttg
    10261 atcgcgagcc gttcggcggg cggcgtcgtg atcagggaca ggtcgcgcag tccgccgatc
    10321 gccatgttga gtgtgcgcgg aatgggcgtc gcggtcaggg tcaggacatg gacttcggcg
    10381 cgcagtgtct tcagccgctc cttgtcgcgc acgccgaagc ggtgctcctc gtcgatgatg
    10441 atgaggccga ggtccttcag gcgcacgttg gcatgcagca gccggtgggt ggccaccacg
    10501 atgtccacgg tgccggcggc gagtccgtcg atcaccgcgt tcgattcctt cgcgctgcga
    10561 aaacgcgaca ggccctcgat gcgcaccggc cagtcggcga agcggtcgcg aaagttcgcc
    10621 acgtgctgct cggcgagcag cgtcgtcggc accagcacca cgacctgcct gccggcctgc
    10681 actgccacga aagccgcgcg catcgccacc tcggtcttgc cgaagccgac gtcgccgcag
    10741 acgatgcgat ccatcggccg atcgctcgcg aggtcctcga tgactttggc gatcgcgtcg
    10801 gcctgctcgg gcgtctcctc gaacgggaac gcgttcgcga agctctggta ctcgagctcg
    10861 cgcagcggca gcgcgaggcc gcgacgggcc tggcggcgcg cgtagagatc gagcagttcg
    10921 gcggcgacgt cgcgtacctg cgcagcggcc ttcttcctcg cgcgggccca ctggtcggtg
    10981 ccgagcttgt gcagcggcgc ggtttcgggc gtggatcccg tgtagcggct gacgaggtgc
    11041 agcgaatgca ccggcacgta gacgcgatcg ccgtcctggt attcgaggac caggaactcg
    11101 ccgggctggt cggcgacgtc catggccgtg aggccgacat agcggccgac gccgtactcc
    11161 tcatgcacca ccggcgcgcc gggggacagg tcgcgcaggt cgcgcaggat cgcctgcggg
    11221 tcgctcgccg cccggcgcct gcggcgctcc tggcgcgcac gcgcaccgaa gagctgcgac
    11281 tcggccagca gggcgatcgg cgggtcgatg agcgtcaggc catcgatctc cggcgcgacg
    11341 gtgagtgcga agcgcgcctc gccgcgcacg aacgcgcccc agccatcgac cggcgtgacc
    11401 ggcgtgtgat gcccgcgcag catctcgacg agcacctcgc ggcgcccggc ggagtcggcc
    11461 gcaatgagta cgcggccctg gtaacctgcc atgaaggcat cgagtggcgc gagcggctgg
    11521 tcggcgcgca cgtcgagccg catctcgcgc ggcgcggcgg tcgggaagtt gcgcacgccc
    11581 gggtcggatt cgccgccgat ctctgccttg aacggatcga tcgtgatgcg cggaagggcg
    11641 tcgagctgcg cgagcagctc gttcggtggc acatagagct cggccggcgc gagcagcggc
    11701 cgctcgatgt cgtggcgatg cgcctcgtgg cgcgcctcga tctcctgcca cgcgcgctcg
    11761 aggtcggctt cgagaccggc accggtcgcg agcagggcgc ccggcggcag gtaatcagta
    11821 agcgttgccg tcgtgtcgtg gaacagcggc aggtagaact cgatgcccgg tggggcgatt
    11881 ccctcgctga ccccgcggta gatctccgag cgtgtcgggt cgccctcgaa acgcgtacgg
    11941 tagcggcgcc tgaaggcgcg cacggcgtcg ggatccagcg gcgtttcgcg cgccggcagc
    12001 aggcgcaccg attcgagggc gtcgagcgaa cgctgcgtgt cggggtcgaa gcggcgtatc
    12061 gcctcgatct catcgtcgaa caggtcgatg cgcagcgggt gcgacgagcc catcgggaac
    12121 acgtcgagca gcgacccacg cagcgcgaac tcgcccggac ccgtcacctg gctgaccgcc
    12181 gcgtagccgg attcgacgag gcgcgtgcgc agcggcacga gcgcgaggcg ctcgccacgg
    12241 gccagccgga agctgcgtcc gatgacgtac tgcgcgggcg gcaggcgacc gagcagcgta
    12301 tcggcggcca cgagcagcac gccatggcgc gcatcccgca ggtcggcgag tgtcgccagg
    12361 cgttccgagg tgatgtcggg atgcggcgag aagcggtcgt agggcagcac ctcccagtcg
    12421 aggagggcga gtatcggcag ccgcccgtcg ctgtagaagc cgatctcggc ggcgcgacgt
    12481 tcgagctcgc gcgcactggc gcagaccacc accagcaggc cgcgcgcgcg cgccgcagcc
    12541 tcggccagcg ccagcgatgg cgcgccgccg tagagctggt gccagcggac atgccggcac
    12601 gacccgtcgg gaatcagcgg ttcaaggatg ttcatcgtcg ccgcgaacgg cgacgcgccg
    12661 ccacggagcg cggcagctta cctgcatcgt gccggaatgg ggagcgatca gccgccgacg
    12721 acgacggcgt ggattacgcg atcgtgctcg aagaataccg agaagcccgc gtaatcccag
    12781 cgggtgatcg gcggctggcc gaccgcggcg tgcttggcgt tcggggcgcc aaagcgctgc
    12841 tccaccgtac tcatcgtgga gccgcggctc ggccgctcga cgctggtctg gcgcaccgcc
    12901 acctggccgt cgaccacgat cgtttccgct gcggcaacgc cgccgaagag cagcccggcc
    12961 acgagccagg gcacagcgcg tgaggacatg cgacacctcc caggagcaag cagcgtgact
    13021 ataacaccgc accccggcag ggcagccgcg gcgcgccggg acccgccatt tatgtgaact
    13081 gcgtcataat gccgcgcagc atgttccatc ccctgtccct gttcgtcggc ctgcgctacg
    13141 tgcgcgcgcg cagccacaag ttcttcgtgt ccttcatcac ctgggtctcg gtggtggggg
    13201 tctgcgtcgg tgtcgcggcg ctgatcgtga ttctctcggt gatgaacggt ttcgaaggcg
    13261 agttgcgcga tcgcctgctc gcgctgtccg cccacgcgcg gctcctgccc gtcggggagg
    13321 aacccgccga cctcgaggcg ctcgccgccg ccgcgagcga ggtgccgggc gttaccggcg
    13381 cggcgcccta tgtcgaagtg caggcgctcg cgctgcgctc gccggagatg ctgccggtcg
    13441 ttttgcgagg catcgatccg gcgcgggaag gggccgtgac cgacatcgcg cgggcggtga
    13501 cggtcggcgc gctcgccgat ctcgtgccgg gaacgaatcg ggtgatcctg ggctcggtgg
    13561 tggcgcagcg gctcaccgcc ggcccgggcg atacgatcac gctgctggtg ccctcgatcg
    13621 gcgcgggcgg cgccccggag ccgcgactgc gcgagttcgc cgtggccggt ctgttcgagg
    13681 tcggtctgca ggaccatgac ggtacgctgg tccttgcggc attcgacgac gtgcgtgcgg
    13741 tgctgccgcg cgccggcaat cccggttccg tgcacctgcg cttcgacgat gcgctcgccg
    13801 cgcccgcgcg cgccgccgcg ctgcgcacgg cgttcgccgg gcaggccgag gtgcgcgact
    13861 ggacccagga ccacgccaac tacttccgtg cgatacgcat cgagaagacg atgatgtcgc
    13921 tgatcctgct gctgatcgtc gcggtggcag cgttcaacat cgtggccatg ctggtgatgg
    13981 tggtgaccga caagcgcacc gacatcgcaa tcctgcgtac gctcggcacg gcgccgcggc
    14041 gcgtgatggg cgtattcgcg acccagggac tcgtgatcgg ctggttcggc gtcatcgccg
    14101 gcattgcgct cggcgtgctg atcgcgcgca acgtcacgcc gatcgtcgcg ttcctcgagc
    14161 gcgccttcgg cttccagatc atggacgccg acgtctacta catcacccgc atcccctcgg
    14221 tgctcgagtg gcacaacgtt gcgtggatcg gcggcgcggc gttcctgctg accgcgctgg
    14281 ccaccgttta tccggcattg cgcgcggcgc gcgtcgcgcc cgccgaagcg ctgcgttacg
    14341 agtagccgat gacacgcttc tacgaatggt ggatcggcac gcgctacctg tcctcgacgc
    14401 accggcgcgg tttcgtgtcg atcgtcgcgc tgatttcggt gatcgggctg atgctggggg
    14461 tcgccgtgct gatcgtggtg ctgtcggtga tgaacggctt cgagcgcgag ctgcgcacgc
    14521 gcatcctcgc ggtcaccgcg cacgcgacgc tgatgggtct cgagggcacg ctcgacgact
    14581 ggcgcgaggc gcgcgccgtg gccctgggca ccgagggggt gcgcgctgcc gtgccctatg
    14641 tcgaggcaca gtcgatgctc gcccacggca gcgtgatcac cggcgcgcag gtgcggggca
    14701 tcgacccgga cgaggagggc aaggccgacg gcctcgagtc gcgcatgacg agcggcagcc
    14761 tcggggcgct gtttcccggc agctggaaca tcgtgctggg agatgcgctc gccgcggaac
    14821 ttggcgcgca ggccggcgac acgctggtac tgatcgcacc ggagggctcg gcgacgccga
    14881 ccggcgtgat gccgcgcatg cgccgcttca cggtcagcgg catattcagt tccggcatgt
    14941 acgagttcga ccgcgggctc gcgctcgtga acctgcagga cgccgcgcgg ctgtatcgga
    15001 ccggcgatgg cgtcaccggg ctgcgcctcg agctcgacga tgcgttcgcc gccccgcgca
    15061 tcgtgcgcga ggtggcgctc ggcctcggcg ggggcttcta tgtcagcgat tggacccgca
    15121 atcacgcgaa tttcttccgc tcgatcgaga tcaccaagtc gctgatgttc gtgatcctgc
    15181 tgatgatcgt cggcgtcgcg gcattcaaca tcgtcgcgac gctggtgatg atcgtcaagg
    15241 agaaacagac cgacatcgcg atcctgcgta ccctcggtgc cggaccgcgc aacgtgctcg
    15301 gtgcgttcac ggtccagggc gtgctgatcg ggcttgcggg caccgcgctc ggcgccggcc
    15361 tcggcatgct ggtcgcacgc aatctcgaat cgctggtgcg cagcctggag cgcgcgctcg
    15421 gcacccagtt cctcgatgcc cgggtctatt acatgagcga tttaccggct tacgtcgaat
    15481 ggcaggatgt gctgcaggtg tgtggagtcg cgttcgcgct gtgcgcgctc gcgacgctct
    15541 atccggcgtg gcgcgccgcg cgcaccgcgc cggccgaggc gctgcgccat gagtgaggcc
    15601 gtcctcgatg cgcgcggcct gcggcgcgag ttcaggcagg gcccggtgct gctcgaagtg
    15661 ctgcgcggca cggaccttgt ggtcggtgtc ggcgagcgcc tcgcgatcat cggcgcctcg
    15721 ggctcgggca agaccacgct gctgcaggtc atcggcgggc tcgaccgtcc gacggccggc
    15781 acggtgctgg tcgacggccg cgatatccat gcggtcggtg agcgggaacg cggcgaattg
    15841 cgcaatcgcg cgatcggatt cgtgtaccag ttccatcacc tgctgccgga gttctcggcg
    15901 ctcgagaacg tggcgatccc gctgctcgtg cggcgcgaac gggtcgccgt cgcacgcgag
    15961 cgtgccgccg cgctgctcga tcgcgtcggg ctcggggcac gactcacgca ccgtccgcac
    16021 cagctctccg gcggcgagcg ccagcgcgcc gcggtggcgc gtgcgctggt gaccgagccg
    16081 cgcctcgtgc tggccgacga gccgaccggc aatctcgacg gcgccaacgc cgaacaggtc
    16141 ttccagttga tgctcgagct gaatcgtgag cggggcacga gcctcgtcgt cgtgacccac
    16201 gacccgcgcc tggcggcccg gatggatcgc gtgctgcggc tcgaggacgg ggtgctggtc
    16261 ccggcctgag gccgctcagc gtcgaaacgg gccgaacggc cccgtttacc gtctggtgcg
    16321 tgccggtcgc gcgcgatatt tctggcgcac gtgaatgcgc cagaacagct cgagcagcgc
    16381 ccagccgagc agcccgccga ccacggcgca ggcgaggcag ccgaccagga acggtttcca
    16441 cagcggcccg agcccgtatt gcagccaggt ccacgagagc tcgaagcgaa agggctgcgg
    16501 tatgtggccg agcatcaggg cgccgacccg ataggcgaag tagtagaccg gcacgaacgt
    16561 aaacgggttg accaggaaca cggtcgccac gagtaccggc gcattgatcc gtgcggtgat
    16621 cgccacgatc gcggcgagga tcaggtgcac cggcagcggc acgaaaccga tggcgatgcc
    16681 ggcgccgaat gccgcagtga ccccgcgccg ctgcagcgac cacagtgccg gatccgcgat
    16741 gcgcgtgccg aacgggcgca gataccagtg gctgcggatc tgctccggcg acggcgtgaa
    16801 tcggcggatc atgtggcggg gcatgtggcg cgccactcta gcagggcggg cgagccatga
    16861 cactcgccgc cgccggtgcg ttcctcgccg gcgcattgct ggcgctcgcc gtgcccacgc
    16921 cgtacccgcc ccatgctgcg ctggccgtgc tgctggccgc acttggcgcc gcgctggcat
    16981 ggcgggcagg gtggccgctt gccactgtgc ttgcggcgtg gctgctggcg gggcttgtct
    17041 gcgcggcggt caccacttcg ctgcggctcg ccgagcgctg gccgcagtcg cgtagcggcg
    17101 aacgcgtact cgtcaccgca cgcgttgcct cgatcccggt cgacgaggaa tacggtaccg
    17161 ccttcgacgc gctcctcacc ctcgatccgc gggccggcgg acagcgcctg cgcgcccgca
    17221 tcgtctgggt gcacccgcag ccccgaccgc aggtcggcga gcgatggcag ctcgcggtgc
    17281 gcctgcgagc gccgcgcgcc gccctgaacc cgaacggccc cgacatggag cgtgtctggt
    17341 tccgggatcg cgtcggcgcg ctcggcacgg tgctgccacg cagcccgctc aacatccgac
    17401 tcgaccccgg tcatgcgccg ctcgatcgac ttcgcgcccg catcatgcgg cgcatcgcgg
    17461 ccaccgtggt cgatcgcgac gcggccgcat tgctgggcgc actggcggtg ggcgtcaccg
    17521 gcgacatgtc gcgcgaacag tggcgcgtgt tcaacgccac cggcaccacg catctcgtgg
    17581 ccatctcggg gatgcacgtg acgctgtttg cggtgatcgc gatggccggc gcgcggcgcg
    17641 tgtggcgggt ggcgggacgc tggtgcaccc ggctgcggcg cgagagcttc gcggcggcgc
    17701 tcggcatcgt cacggcggcc ggctactcgc tgctcgccgg tttctcggtc ccgacgcagc
    17761 gcacgctcct catgttgtcg gcgtggctca tcgcgcgcca cctcgcacgc gcgcacgggg
    17821 cgctacatgc cttcgccgtg gcgctggtcg cggtactgct gctcgatccg ctcgcgccgc
    17881 tcgcgagcgg cttctggctc tctttcggcg ccgtggcggc gattctcctc gccaccggcg
    17941 cgcgcctcgg ccgcgcccac tggctgcatg aggccgtgcg cgtgcagtgc gcggtgacga
    18001 ttgccctcgt acccgtcacg ctggcggcat tcggcagcgt gtcgctcgcg agcctcgtgg
    18061 taaatgccgc cgcgattccg ctgttcacgc tgctgctcgt gcccctcgtc ctcgcggcca
    18121 cggctgcgat ggccctttgg cccgcgctcg ccaccctgct gctgcagctt gcggcctggc
    18181 tgcatgcgca gctgtggccg tggctcgaag ccgcggccga ctggccgcat gcgctcgtgc
    18241 agctcgcgcc accggcatgg gcctacctgc tggtcgtgcc cgccgcaggc atggcggtgc
    18301 tgccctggcc acgcggcctg cgcgccaccg cggcggcggc aattctgccg ctcggcttcg
    18361 cgcgcgcgct cgtgcccgct gccgacgaga tcacggttgc ggtgttcgat gtcgggcgcg
    18421 gccaggccgt cgcgatccgc acggccgatg gcgtgctcgt gcatggcacc ggcgacagct
    18481 tcgggacgga gggacggcgc atggcgcgag tggtcgtgcc atggctacgc acgcagcgcg
    18541 tggcccgcgt cgatgacctc gtgctgccgc acctgtaccg cgattacgcg gtcggcgccg
    18601 ccgagctcga tatcacattg ggcatcgacc gcatcctggt ggcgcgaccc tggcccggcg
    18661 gtccgcagcg cgcggtgacc tgcgatgcgc acgcgggctg gacccgcgca gcggcgcact
    18721 tcgcactgac tgcaacttgc gacatgacgg tgacggtcgg tgcgaaccgg ctcgaggtca
    18781 ccggcaagga ggtgttcctg gacacaggcg cgggtcgcca gcacctcgac agcgaggtcc
    18841 gcggcgcgat catcctgacc ctgggcaagg cggccggtgc ggttcggcgc cgcgatgccc
    18901 gcgacggcta tccatggcca tggcgtgctc cggtatgatg cgcacgggtc gcaaaacagc
    18961 gaggatcgag cggatgtggg aaatcgtgcg cgccggcggg ccactcatgt ggccgatcat
    19021 cctgtgttcc ataggcgctg cggcgatcat cctggagcgc ttctggaccc tgcaggacaa
    19081 aagggtcatt ccgcccgacc tcacccgcaa ggtctcgcag ctcatcgagg gcaaccagat
    19141 caacgacaag gtgatcgccg cgctcgaaca gaattcgccg ctcggccgca tcctcgccgc
    19201 ggggctcgcc aaccgccacc ggccgcgcga gatcatggtg gaacgtctgc aggacacggg
    19261 ccgccacgtc atctatgagc tcgagcgctt cctcaattcg ctcaacatga tcgcgggcgt
    19321 cagccccttg ctcggcctgc tgggcaccgt gaccggcatc atcaaggcgt tcaacgccat
    19381 ccaggcgggc ggcatgggtg acccgcgcat gctctccggc ggcatcgccg aggcactgat
    19441 cacgaccgcc gcgggcctgt ccgtcgcgat tccctcgctg atcggttttc ggctgctgcg
    19501 cggcaaggtg gaccgcatcg tgatccagat ggagaaggac gcattgcgcc tcgccgacga
    19561 agtcgatgcg gcggccggtc gtggcagggc gggcgccgcg tgaacttcaa gccgcgccgc
    19621 acggacgagc ccgagctcaa catgacgcca ctcatcgacg tggtgctgct gctcgtcgtc
    19681 ttcttcatgc tgtcgtcgac cttcgtgcag gaagggcgcc tgcgcgtgcg cctgcccgag
    19741 gcgcgcgacg tgccggtgac ccgtcccgcg caggagccca tcgtcgtgag cgtgaccgag
    19801 gggggtggct atcgcgtcaa tgagcgcgat ctcgtcaacg caggcgccga taccctgagg
    19861 gcggcgctgc tcgcggtggc aggggaggag cgtggcattc cggtcacgct gcgtgccgac
    19921 gcgcgtgcga gccaccaggc cgtggtcacc gcgatggacg tgctcggcaa gctcggtttc
    19981 gccgagatca acatcgcgac ggtgaacgac gcggacgccg ccggcccgtg accgcatcgc
    20041 cgcccgtcgt cgcgaatccc gggcaggtct atcgccgcct gctcgggatt cgcgacgacg
    20101 ggcggcgatg cggtcacggg ccggcggcgt cc
//
LOCUS       DAOQVY010000038        20034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_3147_length_20034_cov_5.711797, whole genome shotgun sequence.
ACCESSION   DAOQVY010000038 DAOQVY010000000
VERSION     DAOQVY010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 20034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20034
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3147_length_20034_cov_5.711797"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PKN91_05350"
     CDS             complement(<1..811)
                     /locus_tag="PKN91_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNS27536.1"
                     /translation="MSRAAQPVREFEFDADNARLADLCGPLDENLKLVESRLGVRIRR
                     RGNIFRVTGAQADAAENVLRALLARSAAGGAGTGATPEEVHLALREEERDADRPLHPE
                     VNAGEPSAGGRDAGRGDGAILRVPRGLIRPRGPHQREYVRNILARDLTFGIGPAGTGK
                     TYLAVACAVEALHADRVRRIVLTRPAVEAGERLGFLPGDLSQKVDPYLRPMYDALYEM
                     FGFDATARLIERNVIEVAPLAFMRGRSLNDSFVILDEAQNTTIEQMKMFLTR"
     gene            complement(808..2175)
                     /gene="miaB"
                     /locus_tag="PKN91_05355"
     CDS             complement(808..2175)
                     /gene="miaB"
                     /locus_tag="PKN91_05355"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNS27537.1"
                     /translation="MDPLPPVPGRYFLKTFGCQMNEYDSARMADVLHAAEGLVATDDP
                     ARADLLLVNTCSVREKAEDKVYSLLGEWRLLKQQRPEVLIGVAGCVASQEGEAIIARA
                     PFVDLVFGPQTLHRLPAMIGDVKRTGRPVVDISFPEIEKFDHLPEPRAAGATAFVSIM
                     EGCSKYCSFCVVPYTRGDEVSRPFGDVLAEVRQLAAQGVAEVTLLGQNVNAYAGQMAD
                     GAIVDLATLIHHVATVAGIERIRFTTSHPLEFTDSLIEAFANVPQLANHLHLPVQSGS
                     DRILALMKRGYTTLEFKDKLRRLRAVRPDIAISTDLIVGFPGETERDFEATLRLVREA
                     ALDQSFSFLYSRRPGTPAASLPDDVPYTEKQARLARLQAQLDAQARAISSAMVGSVQR
                     VLVERPSKKSTQELAGRTENNRWVNFAGPPELIGRFADVTITAALRNSLRGRLLDSPL
                     RAVAS"
     gene            complement(2250..2699)
                     /gene="ssb"
                     /locus_tag="PKN91_05360"
                     /pseudo
     CDS             complement(2250..2699)
                     /gene="ssb"
                     /locus_tag="PKN91_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(2811..3194)
                     /locus_tag="PKN91_05365"
     CDS             complement(2811..3194)
                     /locus_tag="PKN91_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005416164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNS27538.1"
                     /translation="MHRSALAGFIIDCRVDDLPSAAAFWGAALGRELRPSSAEEDAGY
                     VPLEIREDGLHIEVQRVSHESRLHLDIITDDVEAEVQRLENLGARRVQQVRDWWILAA
                     PTGHRFCVIPLRKDGDFPNPPTVWP"
     gene            complement(3298..3912)
                     /locus_tag="PKN91_05370"
     CDS             complement(3298..3912)
                     /locus_tag="PKN91_05370"
                     /inference="COORDINATES: protein motif:HMM:NF017819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HNS27539.1"
                     /translation="MTQASAESILDFWFGPAARDPVAAGERAGMWFGSSAATDEAIRS
                     RFMPTLEAATRGDLAAWQAEPRGALALVLLLDQFPRNAWRGSARAFDCDPQALATSRA
                     AVAASHLARLSPVEQAFMILPFEHSEALADQHECVRLFCDLAQSAPEPWQALLGGYLQ
                     YARQHLEIIERFGRFPHRNRVLGRESTAAELEYLHAGGAVFGQG"
     gene            3982..5127
                     /locus_tag="PKN91_05375"
     CDS             3982..5127
                     /locus_tag="PKN91_05375"
                     /inference="COORDINATES: protein motif:HMM:NF022646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HNS27540.1"
                     /translation="MRRALLPLLPFALLLAPGAVIAASAPAAEAVTSVTPATSLTAAA
                     AERFAQLALDCVQREYPNKISHVLASDEDVRAPRALTPVFYGCFDWHSDVHAHWLLAR
                     LARLYPDTPLASAARTALARSLTDTGIAAEVAYLSGPGRASFERPYGLAWLLALGAEL
                     RAWDDDDARGWAATLAPLEQAAVERLVTWLPKLAYPIRTGEHNQTAFSFGLVWDWAAA
                     TGDDAVLSLLRDKAAQFYLADADCPVRYEPSGEDFLSPCLAEADFLRRVLAPAEFAAW
                     LRDFLPGIPRSRQPWLAPGIVTDRSDPKLAHIDGLNLSRAWMLRGIAAGLPQGDARRA
                     ALLAAAEEHAAAALPAVTGEHYEGGHWLGTFAVYLLSAGGGESSGTP"
     gene            complement(5136..5570)
                     /locus_tag="PKN91_05380"
     CDS             complement(5136..5570)
                     /locus_tag="PKN91_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNS27541.1"
                     /translation="MRPSTAAAVVARCSQNAAGAGRAAAGTHDAAALAGLERDLRYWR
                     ARKASARPVTVATVPEAMRFGVTATLRAADGATRTITIVGEDEADPARGLVSWVSPAA
                     ESLIGAGIGEDVAIGGQRYELIELAAPDATRDATGVAPRDDA"
     gene            5557..7605
                     /locus_tag="PKN91_05385"
     CDS             5557..7605
                     /locus_tag="PKN91_05385"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27542.1"
                     /translation="MLGLTVLASGPAPALAQTAAGTVIRNIAAVRFSGAGGQPEIIHT
                     NEANLTVLPPPSRATLRLLRAATGANASADSILASASVTQCLGANGSEMLPPPSLPGG
                     APLDPQQPLTLVATTVVHGGEPLYFLLHDTDQNLDSTTVDTADVTVTSASGDIEQLRL
                     LESGPDSGEFIGYIQTRAAAANAGDCELQVERNSSLESAYADRSDPADTARAGALVDP
                     FGLVFDARTGAPVDGARIRLVTAGGTSAQVFGDDGISTYPAEMVTGQPVTDSGGTVYH
                     YAAGLFRFPLVAAGTYRLEVVPPLGHAFPSRATEADLQQLPGAPFALGPGSAGDVFVV
                     SGPTPTNVDIPLDPAGSALVLQKASMSAVAAAGDFVQYTLSLRNAGTTGAFTGVTIVD
                     RLPPGVRYRPGSTRRGELRAADPAISADGGVLSFALASLAAGETVVLRYVVEVTVAAR
                     GPELTNSAQAHADGGARSNDAAAVIQLREELFRTHAIVMGRVVAGDCEHEARALPGVA
                     GVRVYLEDGRYAITDEDGKYHFEDVTPGTHVVQLDTVTLPAGREALHCATRVRHAGRA
                     HSQFIDVRGGALWRADFVIPAAITGPAPSPAPAAAATGVARAQAAVSQARATRAPTPD
                     FTLEELEPGNRWLWPDPGRNPQRPLRRRAPPCQACVRPRSRSRVSRARAPRGRNCCMR
                     "
     gene            8211..11081
                     /gene="uvrA"
                     /locus_tag="PKN91_05390"
     CDS             8211..11081
                     /gene="uvrA"
                     /locus_tag="PKN91_05390"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNS27543.1"
                     /translation="MQHIRIRGARTHNLKNIDLDLPRERLIVFTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSMMDKPDVDSIEGLSPAIAIEQKAASHNPRSTVGTVT
                     EIHDYLRLLYARAGTPRCPDHGTDLTAQTVSQMVDQVLAQPQGAAAMLLAPVVEDRKG
                     EHLELFGDLQAQGFVRARIDGRVHEFDALPKLDPKKKHTIEAVVDRFRVRADAAQRLA
                     ESFETALRLSGGTARLAFIDDAARELLFSSRHACPVCGYSVPTLEPKLFSFNNPAGAC
                     PACDGLGTQELFDPKRIVLHPQLSLAGGAVRGWDRHNSYYFQMIQSLARHFDFDIEAP
                     WNELGKRVQDILLFGSGDTRVEFRYAESHGKVRRSRHPFEGIVPNLERRYRETESARV
                     REELAKYLGRRACPECEGSRLNRAARHVFVGDRTLPQLTRLAVGAAHDFFRTLSVAGW
                     RGEVVARIVREVGDRLEFLVNVGLDYLTLDRSADSLSGGESQRIRLASQVGSGLTGVM
                     YVLDEPSIGLHQRDNRRLLATLCHLRDIGNSVIVVEHDEEAILEADYVVDLGPGAGVH
                     GGEIVARGTPEDIKASPDSLTGRYLSGRESVPLPARRTAPDRMRQLRIVGASGNNLKN
                     ITVEIPAGLFTAVTGVSGSGKSTLINDTLYAHVAAHLNGTSVDGGPCERIEGLELIDR
                     VIDIDQGPIGRTPRSNPATYTGLFTPLRELYAQVPEARARGYGPGRFSFNVKGGRCEA
                     CQGDGVLKVEMHFLPDIYVPCDVCKGQRYNRETLEIRYRGRTIHEALEMTVEDALGFF
                     GAIPAIAAKLQTLADVGLAYLRLGQSATTLSGGEAQRVKLSRELAKRATGRTLYILDE
                     PTTGLHFHDVAQLLAVLHRLRDEGNTVVVIEHNLDVVKTADWVIDLGPSGGAGGGTVV
                     ATGTPEDIVRTAGSFTGQYLAPRIAAARATDDTDGIPDHKDH"
     gene            11084..11497
                     /locus_tag="PKN91_05395"
     CDS             11084..11497
                     /locus_tag="PKN91_05395"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HNS27544.1"
                     /translation="MASSKKTKRTAAARARARPRKPDSARRAPLQAYIRAEQGDLSPV
                     DRNYIRRKLGTRLGKFVDSIVRISVRTDDVNGPRGGVDRTCRIKVDLRGLPTQVYEAR
                     AASLEAAIDGALAGIERTVRRTLQRQREQPLKRRR"
     gene            complement(11481..12437)
                     /locus_tag="PKN91_05400"
     CDS             complement(11481..12437)
                     /locus_tag="PKN91_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNS27545.1"
                     /translation="MSKYRHRLPQLSNSFFMTDGGLETTLIYHDGVDLPLFAAFDLLK
                     DEAGTDTLRRYYDRYARLARAHGRGLVLEAPTWRASTDWATKLGYDAPALADANRRAI
                     GLMLEVRAAHESPTSRMVVSGNLGPRGDGYLANAQMSVDAARAYHQAQIDTFAGTDAD
                     MVAAFTMNYVEEAIGIVHAAKAAAMPVVISFTLETDGTLPSGTTLAEAIARTDDETGG
                     APVYYMINCAHPTHFAGVLAGNGDGSAWRNRIRGLRTNASKRSHAELDASTDLDAGDP
                     AELAADHRMLRALLPGLAVIGGCCGSDHRHVDAICAALSAAS"
     gene            complement(12434..13186)
                     /locus_tag="PKN91_05405"
     CDS             complement(12434..13186)
                     /locus_tag="PKN91_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNS27546.1"
                     /translation="MPSPIDLLLDPVTLTSLAIYGALILWEALAPARTLPAVRGWRAK
                     GVAAFALFFLVSSYLPWLWADQLAVWQLLDLSGLNRWAGAVIALLAYQAGVYAWHRSM
                     HGSPRLWRSFHQLHHSAERLDTFGAFWFSPLDMIGWTVLSSLSLGLLGLAPEAVVLFV
                     YFTMFLSIFQHANIRTPRWLGYFVQRPESHSHHHARDVHTGNFGDLPLFDIVFGTFEN
                     PRGFAPETGFYHGASRRVIDMLRFRDIAKEAS"
     gene            13284..14297
                     /locus_tag="PKN91_05410"
     CDS             13284..14297
                     /locus_tag="PKN91_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS27547.1"
                     /translation="MARKIAILAIPDTSASVLYGMYDLFLSAGRDWQLIVEGRPGPKL
                     IDTMVAAARPGAMRVANGVEIVPDCTLADVEDAELICVPEVAVPPGTPLAGRYDTEVA
                     WLRRCHERGATIATACSGALLLAEGGLLRGEEATTHWAYADAMRTGYSGIRVHTRRAM
                     VVAGEGQRLVMAGGGTSWLDLTLYLIARLAGTETAMQTARVNLVEWHEVGQQPYACLA
                     RKSQSEDALIARCQAWIAEHYHEAAPVAAMVRLSGLAERSFKRRFQLATGMSPLEYVH
                     TLRLEEAKQLLETGNDTIEAIANAVGYEDAAFFSRLFRRRVALTPAQYRRRFGAMRRA
                     LGA"
     gene            complement(14302..14820)
                     /locus_tag="PKN91_05415"
     CDS             complement(14302..14820)
                     /locus_tag="PKN91_05415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS27548.1"
                     /translation="MSVTVRDARVLASDRRWIEGVYRDYLDDLAPLNTGIFPALGEIG
                     HREPDQLARWFADPGAKILTVCKSAAPVGFAMVVPGRRLPGRPPVDFHMAEFFIAREH
                     RRLGIGQSAIALIFDRFAGRWEVQEYLRNPVAVKFWRRVVGDYTRGDYEERVEGGEVR
                     QTFRSGPARAPS"
     gene            complement(14918..15226)
                     /locus_tag="PKN91_05420"
     CDS             complement(14918..15226)
                     /locus_tag="PKN91_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27549.1"
                     /translation="MKLKLVFGAALAALMLTACSKPATDTAAEAADTAADAASQAVDA
                     AGEAADAAVDATGAAVDAAADAAADAASQAADAVEGAAADAAQATSDAAQAAADEIKK
                     "
     gene            15391..16716
                     /gene="pepQ"
                     /locus_tag="PKN91_05425"
     CDS             15391..16716
                     /gene="pepQ"
                     /locus_tag="PKN91_05425"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: protein motif:HMM:NF010133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HNS27550.1"
                     /translation="MTHLAPAELYADHLAEIADRTAQALAATGYDALVLHSGGLRMVE
                     FDDYPYPYKAHAAFKLWAPLTDTPDCFVVYRPGERPQLLFNRPDDYWHKATALPDAYW
                     AREFELEPVSTLEAARQALRLEGRTAFIGDVHGFADWGFAAVNPAPLVTRLDHARARK
                     TPYEIACLAAANALGARAHRAAEQAFRAGESEYGIALAFMQSISVREQELPYNPIVAL
                     NAGGAVLHYQVLERAPPAERLSLLIDAGAVHAGYASDITRTYSYADADFAALVARMDE
                     AQLALCAGVKAGVDWRDLHLTAHRLIAEVLHDAGIITVDAEVAVETGLSGVFLPHGLG
                     HLLGLQVHDVGGTLASPEGGTIPRPPGHPYLRLTRVLEQGFVVTMEPGLYFIDSLLAA
                     AKAGPHGRHIDWTRVAQFAPFGGIRIEDNLAVTAAGCDNLTRDAFASLG"
     gene            complement(16713..17108)
                     /gene="mscL"
                     /locus_tag="PKN91_05430"
     CDS             complement(16713..17108)
                     /gene="mscL"
                     /locus_tag="PKN91_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNS27551.1"
                     /translation="MSLASEFKEFAMKGSVIDLAVGVVVGAAFGKIVSSLVGDVIMPP
                     IGMAVGGVNFTDLAVTVGTAADGSEVVLRYGAFLQTIFDFLIIAAALFMAIKLINKLK
                     RPDPAAPPPPPPRDEVLLEEIRDLLKSKS"
     gene            complement(17401..17757)
                     /gene="rplQ"
                     /locus_tag="PKN91_05435"
                     /pseudo
     CDS             complement(17401..17757)
                     /gene="rplQ"
                     /locus_tag="PKN91_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017042856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(17777..18784)
                     /gene="rpoA"
                     /locus_tag="PKN91_05440"
     CDS             complement(17777..18784)
                     /gene="rpoA"
                     /locus_tag="PKN91_05440"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNS27552.1"
                     /translation="MQGSVNEFLKPRVVKVQPIAPRQARVVIEPFERGFGHTLGNALR
                     RVLLSSMPGAAITEIEIDGVLHEYTSIEGVQEDVVDILLNLKQVALRMHTRDSAELRL
                     SKKGPGPVTAGDIQTDHDIEVVNPELVIANLTKAGELTMTLRVERGRGYRPAAQRAAF
                     EEQTRPIGRLQLDASFSPIRRVTYSVDAARVEQRTDLDKLVLDIETNGTIDAEEAIRR
                     AGSILKDQLSVFVDLQGEEEAAAKVSEMQFDPILMRPVDELELTVRSANCLKAENIHY
                     IGDLVQRTEVELLRTPNLGKKSLTEIKEVLQSHGLMLGMRIDGWPPNGLKHDDERSLT
                     L"
     gene            complement(18796..19419)
                     /gene="rpsD"
                     /locus_tag="PKN91_05445"
     CDS             complement(18796..19419)
                     /gene="rpsD"
                     /locus_tag="PKN91_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNS27553.1"
                     /translation="MARYTGPKCKLSRREGTDLFLKSGVKPLESKCKFTVPPGGVKSE
                     RRQRLSDYGTQLREKQKLRRMYGVLERQFANYYVEAASRKGATGENLLKLLECRLDNV
                     VYRMGFAVTRSEARQLVAHKGIEVNGDAVTIASYQVKPGDVVQVREKARKQLRIQNAM
                     QIASQVGLPEWVEVNDKEMKGVFKAVPAREDVLPDINENLVVELYSK"
     gene            complement(19440..19847)
                     /gene="rpsK"
                     /locus_tag="PKN91_05450"
     CDS             complement(19440..19847)
                     /gene="rpsK"
                     /locus_tag="PKN91_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNS27554.1"
                     /translation="MAEQKQTGAQGAKKPKKARKNVLDGIAHIHASFNNTIITITDRQ
                     GNTLSWATSGGCGFRGSRKSTPFAAQVAAEKAGNAAQEHGLRNVEVRVGGPGPGRESA
                     VRALNACGLKITSIADVTPIPHNGCRPPKKRRV"
     gene            complement(19851..>20034)
                     /gene="rpsM"
                     /locus_tag="PKN91_05455"
     CDS             complement(19851..>20034)
                     /gene="rpsM"
                     /locus_tag="PKN91_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNS27555.1"
                     /translation="GDLRREVSMNIKRLMDLGAYRGLRHRKGLPVHGQRTRTNARTRK
                     GPRKQAVKLNAPPGKA"
BASE COUNT         3067 a         7016 c         6742 g         3209 t
ORIGIN      
        1 tgcgggtcag gaacatcttc atctgctcga tcgtcgtgtt ctgcgcctcg tcgagtatca
       61 cgaacgaatc gttgagcgag cgcccgcgca tgaaggcgag cggcgcgacc tcgatcacgt
      121 tgcgctcgat caggcgcgcc gtggcgtcga agccgaacat ttcatagagc gcgtcgtaca
      181 tcgggcgcag gtagggatcg accttctgcg acaggtcgcc cggcaggaaa ccgaggcgct
      241 cgccggcctc gacggccggg cgcgtcagca cgatgcgccg cacgcgatcg gcgtgcagcg
      301 cctcgaccgc gcaggcgacc gcgagatagg tcttgccggt gcccgccggg ccgatgccga
      361 aggtgagatc gcgcgcgagg atgttgcgca cgtactcgcg ctggtgcggg ccgcgcgggc
      421 ggatgagtcc gcgcggcaca cgcaggatcg cgccgtcgcc gcgcccggcg tctctcccac
      481 ccgccgacgg ctcgcccgcg ttgacctccg ggtgcagggg ccggtccgca tcgcgctcct
      541 cctcgcgcag cgcgagatgc acttcctcgg gggtcgcgcc ggtacccgcg ccacctgcgg
      601 cgctgcgcgc gagcagtgca cgcagcacgt tctccgccgc gtccgcctgc gcgcccgtga
      661 cacgaaatat attgccgcga cgcctgatcc gcacgccgag gcgcgattcg acgagcttca
      721 gattctcgtc gagcggtcca cacaggtcgg cgagacgcgc attgtcggcg tcgaactcga
      781 actcgcggac cggttgcgcc gcgcggctca cgaagccacc gcgcgcaacg ggctatcgag
      841 caggcgcccg cgcagcgagt tcctgagcgc cgcagtgatc gtgacgtcgg cgaagcggcc
      901 gatcagctcc ggcggtccgg cgaaattgac ccagcgattg ttctcggtgc gccctgcgag
      961 ttcctgcgtg ctcttcttcg acggccgctc gaccagcacg cgctgcacgc tgccgaccat
     1021 ggcgctgctg attgcgcgtg cctgcgcatc gagttgcgcc tgcaggcgcg ccaggcgcgc
     1081 ctgcttctcc gtgtacggca cgtcatcggg aagcgacgcg gccggcgtac cgggccggcg
     1141 gctgtacagg aagctgaagg actgatcgag ggccgcctcg cgcaccagtc tgagggtggc
     1201 ctcgaagtca cgctccgtct caccgggaaa accgacgatc aggtcggtgc tgatcgcgat
     1261 gtccgggcgg accgcgcgca gccggcgcag cttgtccttg aattcgagcg tggtgtagcc
     1321 gcgcttcatc agcgcgagga tccggtccga gccgctctgc accggaaggt gcaggtgatt
     1381 ggcgagctgc ggcacgttcg caaaggcctc gatgaggctg tcggtgaact cgagtggatg
     1441 cgaggtggtg aagcggatgc gttcgatgcc cgccacggtc gcgacatggt ggatcagcgt
     1501 ggcgaggtcg acgatcgcgc cgtcggccat ctgccccgcg taggcattga cgttctggcc
     1561 gagcagcgtg acctcggcga cgccctgcgc ggcgagctgg cgcacttccg cgagcacgtc
     1621 cccgaacggc cggctgactt cgtcgccgcg cgtgtacggt acgacgcaga agctgcagta
     1681 cttgctgcag ccctccatga tcgagacgaa cgccgtcgcg cctgctgcgc gcggctccgg
     1741 caggtgatcg aatttctcga tctcgggaaa gctgatgtcg acgaccggcc ggccggtgcg
     1801 tttgacgtcg ccgatcatgg cgggcagacg atgcagggtc tgcgggccga acacgaggtc
     1861 gacgaagggc gcccgcgcga tgatcgcctc cccttcctgg ctggcgacgc agccggcaac
     1921 gccgatcagc acctcgggac gctgctgctt cagcaggcgc cactcgccga gcagcgagta
     1981 caccttgtcc tcggccttct cgcgcaccga acaggtgttc accagcagca ggtcggcgcg
     2041 cgccggatcg tcggtcgcga ccaggccctc ggccgcatgc agtacgtccg ccatgcgcgc
     2101 ggagtcgtac tcgttcatct ggcaaccgaa ggtcttgagg aagtagcggc caggcacggg
     2161 gggcaggggg tccaaacccg gcagtttata gatatcgcgc cctcatcggg atcttattca
     2221 gtaaaatcaa tgatttagaa cgggatatcg tcgtcgaaat cgtccttttc ggtggaactg
     2281 gtcaccgggg cctgcgagta ctcgtcgccg gcctggcggc tgcggccgcc accctggcca
     2341 ccggccgcaa ccccgccgcc gccacgcgcg ccgagcatct gcatttcatt ggcgatgatt
     2401 tcggtcgtgt aacggtcgtt gccctgcttg tcctgccact tgcgggtgcg cagcgagccc
     2461 tcgatgtaca cctgcgaacc cttgcgcagg tattcgccgg cgatctcggc aaggcggccg
     2521 aacatggcga cgctgtgcca ctcggtgcgc tccttcatct cgcccgactg cttgtccttc
     2581 cagctctcgc tcgtggcgat gcgcaggttg gtgaccgaac tgccggacgg catggcgcgg
     2641 gtttccgggt cctggcccag gttgccgatg agaatgactt tattgatgcc gcgtgccata
     2701 tggatacctt tcgtgcctgc ggccaagtat gcgtggcggt ggccgcagct gtgaatgatt
     2761 aatcgggtgg aaacgcgcgg attctttcac gccccgcccc gcagggccag tcagggccag
     2821 accgtcggcg gattcgggaa gtcgccatcc ttgcgcagcg ggatgacgca gaagcggtgc
     2881 ccggtcgggg ccgcgaggat ccaccagtcg cgcacctgct gcacgcgccg cgcgccgagg
     2941 ttctcgaggc gctgcacctc ggcctccaca tcgtcggtga tgatgtcgag gtgcaggcgg
     3001 ctctcgtggg acacgcgctg cacctcgatg tgcaggccat cctcgcggat ctcgagcggc
     3061 acatagcccg cgtcttcctc ggcggacgaa ggccgcagtt cgcgacccag cgccgcgccc
     3121 cagaaagcgg ccgcgctcgg caggtcgtcc acccggcaat cgatgatgaa cccggccagc
     3181 gcgctgcgat gcatgccggc agtttaccgc acacccgagg ggtcctggca ggcggacatg
     3241 ctgccgatgc cgcgcccgga agtgctcacg gcggacgacc ccgatgccgc cgcgcgctca
     3301 gccctgcccg aacacggcgc cgcccgcgtg caaatactcg agctccgccg ccgtggactc
     3361 ccgcccgagc acgcgattgc gatgcgggaa gcggccgaag cgctcgatga tttcgaggtg
     3421 ctggcgcgca tactgcaggt agcctccgag cagcgcctgc cagggctcgg gagccgattg
     3481 cgcgaggtca caaaagagcc gcacacattc atgctggtcg gcgagtgcct cgctgtgctc
     3541 gaacggcagg atcatgaacg cctgttccac cggcgagagc cgcgccagat ggctcgccgc
     3601 gacggccgcg cgagatgtcg ccagcgcctg cggatcgcag tcaaaggcgc gcgcgctgcc
     3661 gcgccacgca ttcctcggga actggtcgag cagcaatacc agtgccagcg caccgcgggg
     3721 ctcggcctgc caggcggcga gatcgccgcg cgtggccgct tcgagcgtgg gcatgaaccg
     3781 gctgcggatc gcttcgtcgg ttgcggcgga actgccgaac cacatgcccg cgcgctcacc
     3841 ggccgcgacg ggatcccgtg cggccgggcc gaaccagaag tcgaggatcg attcggcgga
     3901 tgcctgcgtc atcgcgccat agtcgcccac gcacggcgaa actgccacac ttcggcgaaa
     3961 ccacccgctc gccgaggccc catgcgtcgc gccctgctcc cgttgctgcc gttcgcgttg
     4021 ctgcttgcac caggtgctgt cattgccgca tcggccccgg ccgccgaagc cgtcacgtca
     4081 gtcacgccag ccacttcgct gaccgccgcc gcggccgagc gcttcgcgca gctcgcgctc
     4141 gattgcgtgc agcgcgagta cccgaacaag atctcgcatg tcctcgcgag tgacgaggac
     4201 gtgcgcgcgc cgcgcgcgct gaccccggtg ttctacggct gcttcgactg gcactccgac
     4261 gtgcacgcgc actggctgct cgcgcgtctc gcacgcctgt atcccgacac gccgctcgcc
     4321 tccgcggcgc gcacagcgct cgcgcgcagc ctcaccgaca ccggcattgc cgccgaagtc
     4381 gcttacctct ccgggccggg gcgcgcttcg ttcgagcggc cctacggact cgcgtggctg
     4441 ctcgcactcg gcgcggaact gcgcgcgtgg gacgacgacg atgctcgcgg ctgggcggcc
     4501 acgctcgcgc cgctcgagca ggctgcggtg gagcgtctcg tcacctggct gcccaagctc
     4561 gcctacccga tccgcaccgg cgagcacaac cagaccgcct tttccttcgg cctcgtgtgg
     4621 gattgggcgg cggcgacggg cgacgatgcc gtgctgtcgc tgctgcgtga caaggccgcg
     4681 cagttctacc tcgcggatgc cgactgcccg gtgcgctacg agccctcggg cgaggatttc
     4741 ctctcgccgt gcctcgccga ggcggacttc ctgcgccgcg tgctcgcgcc ggccgagttc
     4801 gccgcgtggc tgcgggactt cctgcccggc attccgcgca gcaggcagcc gtggctcgcg
     4861 ccgggcatcg tcaccgaccg cagcgatccg aagctcgcgc acatcgacgg tctgaacctg
     4921 agccgcgcat ggatgctgcg cggaatcgca gccggcttgc ctcaaggcga tgcgcgccgc
     4981 gccgcgctgc tcgccgcggc ggaagagcac gcggctgccg ccctgcccgc cgtgaccggc
     5041 gaacattacg aaggcggcca ctggctcggt accttcgcgg tctacctgct cagcgccggc
     5101 ggcggagaat cttccggaac accctgaggg cgttgtcacg catcatcgcg cggtgcgacg
     5161 ccggtcgcat cgcgggtggc gtccggcgcg gcgagctcga tgagttcgta gcgctggcca
     5221 ccgatcgcca cgtcctcgcc gatcccggcg ccgatcaggg attccgccgc cggtgacacc
     5281 cagctcacca gacctcgcgc agggtcggct tcgtcctcgc cgacgatggt gatcgtgcgc
     5341 gtcgcgccgt ccgccgcgcg cagcgtcgcg gtgaccccga agcgcatggc ctccggcacc
     5401 gtagcgacgg tcaccggccg ggcgctcgcc ttgcgcgccc gccagtagcg caggtcgcgc
     5461 tcgaggccgg cgagcgcggc ggcgtcgtgg gtcccggcgg cggcacggcc cgccccggcg
     5521 gcgttttggc tgcatctcgc gacgaccgcg gcagctgtgc tcggtctcac ggttctggcg
     5581 agcgggcccg cgccggcgct tgcgcagacc gccgcaggca cggtgatccg caatatcgcc
     5641 gcggtgcggt tcagcggcgc cgggggccag cctgagatca tccacaccaa cgaggcgaac
     5701 ctcacggtgc tgccgccacc gtcgcgcgcg acgctgcgac tgctgcgtgc agcgacgggg
     5761 gcgaacgcga gcgccgactc gatcctggca tcggcctccg tcacgcaatg cctcggcgcg
     5821 aacggcagcg agatgctgcc gccgccgtcg ctgccgggcg gcgctccact cgacccgcag
     5881 cagccgctga cgctggttgc gaccacggtc gtgcatggcg gcgagccgct gtacttcctg
     5941 ttgcatgaca ccgaccagaa cctcgactcc accaccgtcg acacggcaga tgtcaccgtg
     6001 acctcggcga gtggcgacat cgagcagctg cgacttctcg agagcggacc cgacagcggc
     6061 gagttcatcg gctacatcca gacccgcgcc gccgcggcga acgccggcga ttgcgaactg
     6121 caggtcgagc gcaactcgag cctcgagtcc gcctacgccg atcgcagcga tcccgccgac
     6181 acggcgcgtg ccggtgcgct cgtcgatccg ttcggcctcg tattcgacgc gcgtaccggt
     6241 gcgccggtgg acggcgcgcg catacggctc gtcacggcgg gcggcacgtc ggcgcaggtg
     6301 ttcggcgacg acggcatcag tacctacccg gccgagatgg tcaccggcca gccggtcacg
     6361 gattcgggcg gcaccgtcta ccactacgcg gcgggcctgt tccgctttcc gctggttgcg
     6421 gcgggcacct accgactcga agtcgtgccg ccgctcggcc atgccttccc ctcgcgggcg
     6481 acggaggccg acctgcagca actgcccggc gccccgttcg cgctgggccc gggatcggcg
     6541 ggcgatgtct tcgtcgtgtc cggtccgacg ccgacgaacg tcgacatccc gctcgatccc
     6601 gcaggcagcg cgctcgtact gcagaaggcc agcatgagcg cggtggcggc ggcgggcgac
     6661 ttcgtgcagt acacgctcag cctgcgcaac gcgggcacca cgggcgcctt caccggcgtc
     6721 acgatcgtcg accggctgcc ccccggtgtg cgctatcgcc ccggttcgac gcgccgcggc
     6781 gagctacgcg cagcggatcc cgctatcagc gcggacggcg gcgtgctcag tttcgcgctc
     6841 gccagcctcg cggccggcga gaccgtggtg ctgcgctatg tcgtcgaagt caccgtggcc
     6901 gcgcggggcc ccgaactcac gaacagcgcg caggcgcatg ccgacggtgg ggcgcgctcg
     6961 aatgatgcgg cagccgtgat acagctgcgc gaggaactgt tccgcactca cgcgattgtc
     7021 atgggccgtg tcgtggcggg tgactgcgag catgaagcgc gtgcgctgcc gggcgtcgcg
     7081 ggcgtgcgcg tctacctcga ggacggccgt tacgcgatca ccgacgagga tggcaagtac
     7141 cacttcgagg acgtcacgcc gggcacgcac gtcgtgcagc tcgacaccgt gacgctgccg
     7201 gccggccgcg aggccctgca ctgcgccacg cgcgtgcgtc atgccggacg cgcacactcg
     7261 cagttcatcg acgtgcgcgg cggtgccctg tggcgcgccg acttcgtcat cccggccgcc
     7321 atcaccgggc cggcgccgag ccctgcacca gcggccgctg caacgggcgt ggcgcgggca
     7381 caggccgccg tgagccaggc tcgcgcgacg cgcgccccca cgccggactt caccctcgaa
     7441 gaactcgagc ccggcaaccg gtggctgtgg ccggaccccg gtcgcaaccc gcaacgcccg
     7501 ctccggcgac gagcaccgcc atgtcaagcg tgtgttcgac cgcggtcgcg gtcgcgcgtg
     7561 tcccgcgctc gagcaccgcg tggccgaaat tgttgtatga ggtgaactgc cactggtatt
     7621 cggcgaaggc ggccacggtc gcggcgtcga gcggccgcgt attgagccgc accgatgccg
     7681 ggtcgtacca cgcgcagcga tagacacgca tgcgcacata gtcgttggcg accgtgcggg
     7741 gcacccgtcc gaattcgaag tagcgcgcct cttcggtcgt cgcgaccagg tcggagtacg
     7801 tgggcgccgc gagcgctgcg gcctcggagg ccgcgagcgc cgtattccag tcgtccgagc
     7861 aggccgcgag atggtaggtg gtgacctgcc cgtaaccgga cggcaccggg tcgagcgcga
     7921 agccctccgg agtccgttgg cggtcgctgt agaggagatt ggcgacctgc aggtcggaca
     7981 actccacggg ccgcgccgcc tgcgcgggtg cgcccggcgc gggcacggac gactccccgc
     8041 ccccaccgca accgaccagg accagcacta tgccccacca gaaccgcatg aatgggcctc
     8101 ctgccggggt tatcggcgcg gccggcggcg acttgagtcg ccgggacacc tgagggtcgt
     8161 cgggcggtaa acttaatggt ttgatccggg ccctccggat tggatggggc atgcaacaca
     8221 tccgcatccg cggcgcgcgg acccacaacc tgaagaacat cgacctcgac ctgccacgcg
     8281 agcggctgat cgtgttcacg ggcctgtcgg ggtcgggcaa gtcctcgctc gccttcgaca
     8341 cgctctatgc cgaggggcag cgccgctatg tcgaatcgct gtcggcctac gcccgccagt
     8401 tcctgtcgat gatggacaag cccgatgtcg actcgatcga ggggctctcg cccgcgatcg
     8461 cgatcgagca gaaggccgcc tcgcataatc cgcgctcgac ggtcggcacc gtgaccgaga
     8521 tccacgacta cctgcgcctg ctgtatgccc gggccggcac gccccgctgc cccgaccacg
     8581 gcaccgacct caccgcccag accgtgagcc agatggtcga tcaggtcctc gcgcaaccgc
     8641 agggcgcggc cgcgatgctg ctcgctcccg tggtcgagga ccgcaagggc gagcacctcg
     8701 aattgttcgg cgacctgcag gcacagggct tcgtgcgcgc gcgcatcgac gggcgcgtgc
     8761 acgagttcga cgccctgccg aaactcgacc cgaagaagaa gcacaccatc gaggcggtgg
     8821 tcgatcgttt ccgcgtgcgc gcggacgccg cgcagcgcct ggcggaatcg ttcgagacgg
     8881 cgctgcgcct gtcgggcggt accgcacgcc tcgcattcat cgacgacgct gcgcgagagc
     8941 tgctcttctc gagccgccac gcctgcccgg tctgcggcta cagcgtgccg acgctggagc
     9001 cgaagctgtt ctcgttcaac aatccggccg gcgcatgccc ggcctgcgac gggctcggca
     9061 cccaggaact gttcgatccg aagcgtatcg tcctgcaccc gcagctgtcg ctggcgggcg
     9121 gcgcggtgcg cggctgggat cgccacaaca gctactactt ccagatgatc cagtcgctcg
     9181 cgcgccattt cgatttcgac atcgaggcgc cgtggaacga actcggcaag cgcgtgcagg
     9241 atatcctgct gttcggcagc ggcgacaccc gggtcgaatt ccgctacgcg gaatcgcacg
     9301 gcaaggtccg ccgcagccgc catccgttcg agggtatcgt gccgaacctc gaacgccgct
     9361 atcgcgagac cgagtcggca cgcgtgcgcg aggagctcgc caagtacctt ggaagacggg
     9421 cctgccccga atgcgagggc tcgcgactca atcgtgcggc acgccatgtg ttcgtcggcg
     9481 atcgcaccct gccgcagctc acgcggctcg ccgtcggtgc agcgcacgat ttcttcagga
     9541 cgctgtcggt cgccggctgg cggggcgaag tcgtcgcgcg catcgtcagg gaagtcggcg
     9601 accgcctcga gttcctcgtc aatgtcgggc tcgattacct tacgctcgat cgcagtgccg
     9661 attcgctctc gggcggcgag tcgcagcgca tccgcctcgc gagccaggtg ggctccggcc
     9721 tcaccggcgt catgtatgtg ctcgatgaac cgtcgatcgg cctgcaccag cgcgacaatc
     9781 gccgcctgct cgcgacgctc tgccatctgc gcgacatcgg caactcggtc atcgtcgtcg
     9841 agcacgacga ggaggcgatc ctcgaggcgg actacgtcgt cgacctcggc cccggcgccg
     9901 gcgtgcatgg gggtgagatc gtcgcgcgcg gcacacccga ggacatcaag gcgtcacccg
     9961 attcgctcac cggccgctat ctctccggcc gcgagtcggt gcctctgccg gcgcggcgca
    10021 ccgcgcccga ccggatgcgc cagctgcgca tcgtcggcgc gagcggcaac aacctgaaga
    10081 acatcaccgt cgagattcct gccggtctgt tcaccgccgt gaccggcgtg tcgggctcgg
    10141 gcaagtcgac gctgatcaac gacacgctgt acgcacacgt cgcggcgcat ctcaatggca
    10201 cgagcgtcga tggcggcccc tgcgagcgca tcgaggggct cgagctgatc gatcgcgtca
    10261 tcgacatcga ccagggtccg ataggccgca cgccgcgatc gaatcccgcc acctacacgg
    10321 ggctgttcac gccgctgcgc gaactctatg cgcaggtgcc cgaggcgcgc gcccgcggct
    10381 acggccccgg ccgcttcagc ttcaacgtca agggcgggcg ctgcgaggcc tgccagggcg
    10441 acggcgtgtt gaaagtggag atgcacttcc tgcccgatat ctacgttccg tgcgacgtat
    10501 gcaagggaca gcgatacaac cgcgagacgc tcgagatccg ctaccgcggc aggacgatac
    10561 acgaggcact cgagatgacc gtcgaggacg cacttggatt cttcggcgcg atccccgcga
    10621 tcgccgcgaa gctgcagacc ctggccgacg tgggcctcgc ctacctgcgc ctcggccaga
    10681 gcgcgacgac gctgtcgggc ggcgaggcac agcgcgtgaa gctctcgcgg gaactcgcca
    10741 agcgcgcaac tggccgcacg ctctacatcc tcgacgagcc cacgaccggg ctgcatttcc
    10801 acgatgtcgc gcagctgctc gcggtattgc atcgcctgcg cgacgaaggc aatacggtcg
    10861 tcgtgatcga gcacaacctc gacgtcgtga agacggccga ctgggtgatc gacctgggcc
    10921 cgtcgggcgg tgccggcggc ggtacggtgg tcgccaccgg cacacccgag gacatcgttc
    10981 gcacggccgg atcgttcacc ggccagtacc tggcgccccg cattgccgct gcgcgcgcca
    11041 cggacgacac tgacggcatt cccgaccaca aggaccactg accatggcga gcagcaagaa
    11101 gacgaaacgc accgcggccg cacgcgcccg cgcccgtccg cgcaaacccg acagcgcgcg
    11161 ccgcgcaccg ctgcaggcgt acatccgtgc cgagcagggc gatctcagtc ccgtcgatcg
    11221 caactacatc cgccgcaagc tcggcacgcg gctcggcaaa ttcgtggact cgatcgtgcg
    11281 gatcagcgtg cgtaccgacg acgtgaacgg gccgcgtggc ggggtcgacc ggacctgccg
    11341 catcaaggtc gacctgcgcg gcttgcccac ccaggtgtat gaagccaggg ccgcctcgct
    11401 cgaggccgcg atcgacggtg cgctcgcggg catcgagcgc accgtgcgtc gcacactgca
    11461 gcgccagcgc gagcagccgc tcaagaggcg gcgctgagcg cagcgcagat ggcgtccacg
    11521 tgccggtgat cgctgccaca gcaaccgccg atcaccgcga gacccgggag cagcgcccgc
    11581 agcatgcgat gatcggccgc gagttcggcc ggatcgccgg catcgaggtc ggtggatgcg
    11641 tcaagctccg catggctgcg cttcgaggcg ttggtccgca ggccgcggat ccggttgcgc
    11701 caggctgacc cgtcgccatt gcccgcgagc acgccggcga agtgcgtcgg gtgcgcacag
    11761 ttgatcatgt aatacaccgg tgcgccgccg gtttcgtcgt cggtgcgcgc gatcgcctcg
    11821 gcaagcgtcg tcccggacgg cagggtaccg tcggtctcca gcgtgaacga gatcaccacg
    11881 ggcatcgctg cggctttcgc cgcgtgcacg atcccgatcg cctcctccac atagttcatc
    11941 gtgaatgcgg cgaccatgtc ggcatccgtg ccggcgaagg tgtcgatctg tgcctggtgg
    12001 taggcacgcg ccgcatcgac gctcatctgc gcattggcga gatagccgtc gccgcgcggc
    12061 cccaggttgc cgctcacgac catgcgcgat gtcggcgact cgtgggccgc acgcacctcg
    12121 agcatcagcc cgatcgcgcg gcggttcgcg tcggcgagtg ccggggcgtc gtagccgagc
    12181 ttcgtcgccc agtcggtgct cgcacgccag gtgggcgctt cgagcaccag accgcgtccg
    12241 tgcgcgcgcg cgagccgcgc atagcgatcg tagtagcggc gcagtgtgtc ggtgcccgcc
    12301 tcgtccttga gcaggtcgaa ggcggcgaac aacggcaggt ccacgccgtc gtgatagatc
    12361 agcgtcgtct cgagtccgcc gtcggtcatg aagaacgaat tcgagagttg cggcagtcgg
    12421 tgccgatact tgctcatgat gcctccttgg caatatccct gaatcgcagc atgtcgatca
    12481 cgcgccgcga cgctccgtgg tagaaaccgg tttccggcgc gaacccgcgc ggattctcga
    12541 aagtgccgaa cacgatatcg aacagcggca ggtcgccgaa gttgcccgtg tgcacgtcac
    12601 gcgcatggtg gtgcgagtgg ctctcggggc gctgcacgaa gtagccgagc cagcgcggtg
    12661 tacggatgtt ggcgtgctgg aagatcgaca ggaacatcgt gaagtacacg aacagcacga
    12721 ccgcctcggg cgcgagcccg agcaggccga gggacaggct cgacagcacg gtccagccga
    12781 tcatgtcgag cgggctgaac cagaacgcgc cgaaagtgtc gagacgctcg gcgctgtggt
    12841 gcagctggtg gaagctgcgc cagagacgcg gcgagccgtg catgcttcga tgccacgcat
    12901 agaccccggc ctggtacgcg agcagcgcga tgaccgcccc ggcccagcgg ttgaggcccg
    12961 acaggtcgag cagctgccag acggcgagct ggtcggccca cagccagggc agataagacg
    13021 aaaccaggaa gaaaagcgcg aatgcggcga cgcctttcgc gcgccagcct ctcacagcag
    13081 gtagagtacg ggcaggagcc agcgcctccc acaggatgag cgcgccgtag atggcgaggc
    13141 tcgtcagcgt tacggggtcg agaagcaggt cgatcgggct cggcatgatt tcgtccttga
    13201 agccgttcag gcggggcgga agattagtcg cagggcgccc gcggcactaa agccataccg
    13261 gcaaggaagc ggacatttcg gccatggcca gaaagattgc catcctcgcg atacccgaca
    13321 cctcggcgtc ggtgctctac ggcatgtatg accttttcct gtcggccggg cgcgactggc
    13381 agttgatcgt cgagggccgg cccgggccga agctgatcga cacgatggtc gccgccgccc
    13441 gccccggtgc catgcgcgtt gcgaacggcg tggagattgt cccggactgc acgctcgcag
    13501 acgtcgagga cgccgagctg atctgcgtgc ccgaagtcgc cgtgccgccg ggcacgccgc
    13561 tcgccgggcg ctacgacacc gaagtcgcct ggctgcgccg ctgccatgag cgtggcgcga
    13621 ccatcgccac ggcctgctcc ggtgcgctgc tgttggccga aggcgggctg ctgcgcggcg
    13681 aggaagcgac gacgcactgg gcatatgccg acgccatgcg caccggctac agcggcatcc
    13741 gcgtgcacac ccggcgcgcc atggtggtcg ccggggaagg ccagcgcctc gtcatggcag
    13801 gtggtggcac ctcctggctc gacctcacgc tctacctgat cgcgcgtctc gccggcaccg
    13861 aaaccgccat gcagaccgcg cgcgtcaatc tcgtcgagtg gcacgaggtc ggccagcaac
    13921 cctatgcctg cctcgcccgc aagagccagt cggaggatgc gctcatcgcc cgctgccagg
    13981 cctggatcgc cgagcactac cacgaggcgg cgccggtggc tgcgatggtg cgcctgtcgg
    14041 gcctggccga gcgttcgttc aagcgacgct tccagctggc gaccggcatg tcgccgctcg
    14101 agtacgtgca cacgctgcgc ctcgaggagg cgaaacagct gctggaaacg ggcaatgaca
    14161 cgatcgaggc gatcgcgaat gcggtgggtt acgaggacgc ggcattcttc tcgcgcctct
    14221 tccggcgccg cgtggcactg acaccggcac aataccggcg ccgcttcggc gccatgcgcc
    14281 gcgcgctcgg cgcctgagcc gctagctcgg cgcgcgcgcg ggacccgaac ggaacgtctg
    14341 gcgcacttcg ccgccttcga cgcgctcctc gtagtcgccg cgcgtgtagt cgccgaccac
    14401 gcggcgccag aatttcacgg ccacggggtt gcgcaggtat tcctggactt cccagcgtcc
    14461 ggcaaagcga tcgaagatca gtgcgatggc gctctgcccg atgccgagcc ggcgatgctc
    14521 gcgcgcgatg aagaactcgg ccatgtggaa gtcgacgggg ggtcggccgg gcagcctgcg
    14581 ccccggcacc accatggcaa aaccgacagg tgcggcggac ttgcacaccg tgaggatctt
    14641 cgcacccggg tcggcaaacc agcgcgctag ctggtccggt tcacggtggc cgatttcccc
    14701 gagtgcgggg aagatgccgg tgttgagcgg cgcgagatcg tcgaggtaat cgcggtacac
    14761 gccctcgatc catcgccggt cggaggcgag gacgcgcgcg tcgcgcaccg tcaccgacat
    14821 gccggggtcc gcagacaaca aggcccggac aggcgatgta actcgccggc cgggccctgt
    14881 gtgagccaaa ggctcttcaa gcggtaactc gttcgaatta cttcttgatc tcgtcggcag
    14941 cagcctgcgc ggcgtcgctg gtcgcctggg cggcgtccgc agccgcaccc tcgaccgcat
    15001 cggcggcctg gctggcagcg tcggcagcgg catcggcagc ggcatcgacc gcggcgccag
    15061 tcgcgtcgac ggcggcatcg gcagcctcac cggcggcgtc gacggcctgg ctggcggcat
    15121 cggcggccgt atcggcagcc tcggcggccg tgtcagtcgc cggcttgctg caagcagtga
    15181 gcatgagcgc cgcgagcgcg gcaccaaata cgagtttgag cttcatggat cgttacccca
    15241 gaaaagtaga agcgagagtg gtttatatgc aggaatgcgc gcgattttca tcgcgcgcgc
    15301 aaattctata gagtcccatc atctttttaa accgcgcggc aggactgtcg gcccacagct
    15361 acagtcggaa tccgcctacg agacccccaa atgacccacc tcgcgcccgc cgagctgtat
    15421 gccgaccacc tggccgagat cgccgaccgc acggcacaag ccctcgccgc gaccggctac
    15481 gacgcgctgg tgctgcactc gggcggcctg cggatggtcg aattcgacga ctacccctac
    15541 ccctacaagg cgcatgccgc cttcaagctc tgggcgcctc tcaccgacac gcccgactgc
    15601 ttcgtcgtct accggcccgg cgagcggccg cagctgctgt tcaaccgccc cgacgactac
    15661 tggcacaagg ccaccgccct gccggacgcc tactgggccc gtgagttcga attggagccg
    15721 gtctccaccc ttgaagccgc gcgccaggcg ctgcggctcg aggggcgcac cgccttcatc
    15781 ggcgacgtac atggctttgc cgactggggc ttcgccgcgg tgaaccccgc gccgctcgtg
    15841 acccgcctcg accatgcgcg tgcccgcaag actccgtacg agatcgcctg cctcgctgcg
    15901 gcgaacgcgc tcggcgcccg ggcgcaccgg gccgcggagc aggccttccg cgccggcgag
    15961 agcgaatacg gtatcgcgct cgcgttcatg caatcgattt cggtgcgcga gcaggaactg
    16021 ccgtacaacc ccatcgtcgc gctgaatgcc ggcggcgcag tgctgcacta ccaggtgctc
    16081 gagcgcgcgc caccggccga gcgtctgtcg ctgctgatcg acgccggtgc cgtacacgca
    16141 ggctacgcgt cggacatcac gcgcacgtac tcgtacgcgg acgccgattt cgcggcgctg
    16201 gtggcgcgca tggatgaggc gcagctcgcg ttgtgcgcgg gcgtgaaggc gggcgtggac
    16261 tggcgcgacc tgcacctgac cgcccaccgc ctgattgccg aagtgctgca cgatgccggc
    16321 atcatcaccg tcgatgccga ggtcgcggtc gagaccggtt tgtccggcgt attcctgccc
    16381 catggcctgg gtcacctgct cggcctgcag gtgcacgacg tcggcggcac gctggcctcg
    16441 ccggaaggcg gcacgatccc gcgtccgccg ggccacccct acctgcgcct gacgcgcgta
    16501 ctcgagcagg gtttcgtggt cacgatggag cccgggctct acttcatcga ctcgctgctc
    16561 gcggccgcga aggccgggcc ccacgggcgc catatcgact ggacgcgcgt cgcgcagttc
    16621 gcgcccttcg gcggcatccg gatcgaggac aacctcgccg tcaccgccgc aggctgcgac
    16681 aacctgaccc gcgacgcgtt cgcctcgctt ggctaggact tcgacttcag cagatcgcgg
    16741 atctcctcga gcagcacctc gtcgcgcggc ggcggcggcg gcggtgccgc gggatccggg
    16801 cgcttcaact tgttgatcag cttgatcgcc atgaacagtg cggcggcgat gatcaggaaa
    16861 tcgaaaatcg tctgcaggaa ggcgccatag cggagcacca cctccgaacc gtccgccgcg
    16921 gtacccaccg tgacggcgag atcggtgaaa ttgactcccc cgacggccat accgatcggc
    16981 ggcatgatca cgtcccccac cagcgaggag acgatcttgc cgaacgccgc gccgaccacg
    17041 acaccgaccg cgaggtcgat gacgctgccc ttcatcgcga attccttgaa ctccgaggcc
    17101 aggctcatgg ggcatcccct tgtcgttgca ccgcgcacag aatacatgtg atcgggcgcg
    17161 ggcgtctcgg caccaaagaa aaagcccggc ggtgccgggc tctttcgctg ctcgacgcgc
    17221 aaggcgcgtg aatcaggcgc cggccttctt cttcgcgacc ttggccttct tcttcggtgc
    17281 cgcctcggcc ttcgccttgg ccggcttctt cgccgccgcc gtcttcgccg ccgccttctt
    17341 cttcgcagtc ttcgcctgct tgggcgccgg ctcgtccgcc gccccggtgg cagccgcggc
    17401 cgggccatcg accagctgca tcagcgccat cggcgcggag tcgccgggac gcggcatcat
    17461 gtgcaggata cgcgtgtagc cgcccgggcg cgccttgaag cgcggtccca ggtcggcaaa
    17521 cagcttgtcg acgacggctg cgtcgcgcag gcgcgagaac gccagccggc ggttggcatc
    17581 cgagtcgttc ttcgcgagcg tgatcagcgg ctcgacgacc cgacgcagtt ccttcgcttt
    17641 cggcagcgtg gtgcggatgg tctcgtgccg cagcagcgag acgctcatgt tgcggagcat
    17701 cgcgtggcga tgcgggctgt ttcgcgacag ctgccgcccg gacagttggt gacgcatggg
    17761 cttctgttcc tggttcttac agcgtgaggc tgcgctcgtc gtcgtgcttc aggccgttcg
    17821 gcggccagcc gtcgatccgc atgccgagca tgaggccgtg gctctgcagc acttccttga
    17881 tttcggtgag cgacttcttg ccgaggttgg gcgtacgcag cagctcgact tcggtacgct
    17941 gcacgaggtc gccgatgtag tggatgttct ccgccttgag gcagttcgcg ctgcgcaccg
    18001 tgagctcgag ctcatcgacc ggccgcatca ggatcgggtc gaactgcatc tcggagacct
    18061 tggcagccgc ctcttcctcg ccctgcaggt cgacgaacac cgacagctgg tccttcagga
    18121 tgctgccggc ccggcggatc gcctcttcgg cgtcgatcgt gccgttggtc tcgatgtcga
    18181 gcaccagctt gtcgaggtcg gtgcgctgct cgacgcgcgc cgcgtcaacc gagtaggtca
    18241 cgcgacggat cgggctgaac gacgcatcga gctgcagccg gccgatcggc cgcgtctgct
    18301 cctcgaacgc cgcgcgctgc gccgccggcc ggtagccgcg gccgcgctcg acacggagcg
    18361 tcatcgtcag ctcgccggcc ttggtcaggt tggcgatcac cagctccggg ttcacgactt
    18421 cgatgtcgtg gtcggtctgg atgtcgccgg ccgtgaccgg gcccgggccc ttcttcgaca
    18481 ggcggagctc cgcactgtcg cgcgtgtgca tgcgcagtgc cacctgcttc aggttcagca
    18541 ggatgtcgac gacatcctcc tgcacaccct cgatgctggt gtactcgtgc agcacgccgt
    18601 cgatctcgat ctcggtgatc gcggcacccg gcatggatga cagcaggacg cggcgcaggg
    18661 cattgcccag cgtgtgaccg aacccgcgct cgaacggctc gatgacgacg cgcgcctggc
    18721 gaggcgcgat cggctggacc ttgacgacgc gaggcttcag gaactcgttt accgatcctt
    18781 gcatggtaac cacctttact tcgagtagag ctcgacaacg aggttttcgt tgatgtcagg
    18841 gagtacgtcc tcgcgggccg gcacggcctt gaacacgccc ttcatctcct tgtcgttgac
    18901 ctcgacccat tccggcagac ccacctgcga ggcgatctgc attgcattct ggatgcgcag
    18961 ctgcttgcgc gccttctcgc gcacctgcac gacgtcaccc ggcttgacct ggtacgacgc
    19021 gatagtcacg gcatcaccgt tcacttcgat gcccttgtgc gcgacgagct ggcgcgcctc
    19081 ggaacgcgtg accgcgaatc ccatgcggta cacgacgttg tccagccggc actcgagcag
    19141 cttcagcagg ttctcgccgg tcgcgccctt gcggctcgcg gcttcgacgt agtagttcgc
    19201 gaactggcgc tcgagcacgc cgtacatgcg gcgcagcttc tgcttctcgc gcagctgcgt
    19261 gccgtagtcg gacagccgct ggcggcgttc gctcttcacg ccgcccgggg gcaccgtgaa
    19321 cttgcacttg ctctcgagcg gcttgacgcc gctcttgagg aacagatcgg taccttcgcg
    19381 acgcgaaagc ttgcacttgg ggcctgtgta acgtgccatg tatcagtgac ctctgcgcgt
    19441 cagacgcggc gcttcttcgg cgggcgacag ccgttgtggg ggatcggcgt gacatccgcg
    19501 atgctggtga tcttgagacc gcaggcgttc agcgcgcgca cggccgattc gcggccgggg
    19561 cccgggccac cgacccgcac ctcgacgttg cgcaggccgt gttcctgcgc ggcattgccg
    19621 gccttctcgg ccgcaacctg cgccgcgaac ggcgtgctct tgcgcgagcc gcggaaaccg
    19681 cagccgcccg acgtcgccca cgacagcgta ttgccctgcc ggtcggtgat cgtgatgatc
    19741 gtgttgttga aagaggcgtg gatgtgcgcg atgccgtcca gcacgttctt gcgtgccttc
    19801 ttcggcttct tcgcgccctg cgcgcctgtc tgcttctgtt cagccatgtg ttatgccttg
    19861 cccggaggtg cgttcagctt cactgcctgc ttgcgcggac ccttgcgcgt gcgcgcgttg
    19921 gtgcgcgtgc gctggccatg gaccggcagg cccttgcggt gccgaagccc gcgataggcg
    19981 ccgaggtcca tcaggcgctt gatgttcatc gagacttcgc gacgcaggtc gccc
//
LOCUS       DAOQVY010000039        19768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_3200_length_19768_cov_6.716177, whole genome shotgun sequence.
ACCESSION   DAOQVY010000039 DAOQVY010000000
VERSION     DAOQVY010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19768
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3200_length_19768_cov_6.716177"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="PKN91_05460"
     CDS             complement(<1..1253)
                     /locus_tag="PKN91_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS27556.1"
                     /translation="MKGLDAVRKRPGMYIGDTDDGSGLHHMVFEAVDNSVDEALAGYC
                     TRVVVTIHADESITVADNGRGIPVDIHPEEGRSAAEVIMTVLHAGGKFDDTSYKVSGG
                     LHGVGVSVVNALSDYLQMTIARDGKLHRQEYRMGEPQAPLAVVGETTERGTTIRFKPS
                     AKIFTNITFNYEILAKRLRELSFLNSGVRIELVDEREDKSDVFQHEGGLQAFVTHLNR
                     SRTPIHNTVFWFRTQEGPIQVEVALQWNDSYQESMYCYTNNIPQKDGGTHLSGFRAAL
                     TRTLNDYIEREGVAKREKVPTTGDDAREGLTAILSVKLPDPKFSSQTKDKLVSSEVKG
                     IVETAVASKLSEFLLEHPADAKSIVGKIIEAARAREAARKAREMTRRKGALDIAGLPG
                     KLADCQEKDPALSEIFIVEGDSAGGS"
     gene            complement(1323..2393)
                     /gene="recF"
                     /locus_tag="PKN91_05465"
     CDS             complement(1323..2393)
                     /gene="recF"
                     /locus_tag="PKN91_05465"
                     /inference="COORDINATES: protein motif:HMM:NF000819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNS27557.1"
                     /translation="MPLVDLLVEDLRCIERLELTLHPGLNLIAGANGTGKTSLLEAVF
                     LLGRGRSFRTRSSERLIRHGADRLIVFGRTDRHAIGVEVQRGQGHATAVTRAKVDGNF
                     VQALTELTGVFPVQVIEPGVQQLVDGGATGRRRWLDWSVFHVEPQFGVHWARYARALK
                     QRNAALRAGVGPEGLKPWDRELVAAGVPMAEARAAHYAALEPVWMRVQTALLGFTVGA
                     TWSTGWDRERSLEEALAAGAAHDVSRGTTSAGPHRADLRLRMGTRAAREVLSRGQQKL
                     LALSMVLAQLELLRDSLQIRPTLLIDDPAAELDNFRLGAVIERIRALETQLVVTTLAP
                     DSSLFGNPDRVYQMGQGRAETL"
     gene            complement(2401..3498)
                     /gene="dnaN"
                     /locus_tag="PKN91_05470"
     CDS             complement(2401..3498)
                     /gene="dnaN"
                     /locus_tag="PKN91_05470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNS27558.1"
                     /translation="MKLSATRAQVLNPLQSVIGVVERRQTMPILANVLLSARGNRLSI
                     TGTDLEVELVAATEATVQQAGDITVPGRKLLDIFRALPDGVNVTLATEGERVIVRAGR
                     SRFTLSSLPSAEYPVVEEINAQQTLTVPQAEFRRLIEKTHFSMAQQDVRYYLNGLLIE
                     ADGKNLRAVATDGHRLAICDCPLESGNSTAQQVIVPRKGVLELQRLLGAEGNIELAIG
                     TNHIRAQIGDVRFTSKLIDGRFPEYGRVIPASPSRVVEADREPLRQALQRTAILSNEK
                     YRGVRLTVKPDLLTIQAHNPEQEEAEDQIEVSYQGEEVEIGFNVNYLLDALGAIDGDK
                     VQIGLGDANSSCLIRADATSTARYVVMPMRL"
     gene            complement(3714..5084)
                     /gene="dnaA"
                     /locus_tag="PKN91_05475"
     CDS             complement(3714..5084)
                     /gene="dnaA"
                     /locus_tag="PKN91_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNS27559.1"
                     /translation="MAGSTLTGLSLEAGLWARCLRALEDELPEQQFNTWVRPLQAVQG
                     DGALKLLAPNRFVVDWVNGNLLARIGELLRDESTGDAPIVTVEVGSRSSRSATASAPE
                     PAATPRGRRTEGLVVGARLNPDYTFETFVEGKSNQLARAAALQVGENPGKAYNPLFVY
                     GGVGLGKTHLMHAAGHMIRQRNPDARIAYVHSERFVSDMVRALQHNTMNEFKAAYRSL
                     DALLIDDIQFFAGKDRSQEEFFHTFNALLEGQQQVIVTCDRYPKEVTGLEERLKSRFG
                     WGLTVAIEPPELETCVAILMSKGQAAGVALPEEVAFFIAKRIRSNVRELEGALRRVIA
                     NSRFTGRPITLEFAKEALKDLLSLQAKLVTVENIQKTVADYYKVRVADLLSKRRNRSI
                     ARPRQVAMALAKELTSHSLPEIGDAFGGRDHTTVLHACKRIKEFRDSDQRISEDYSNL
                     LRTLTG"
     gene            5180..5317
                     /gene="rpmH"
                     /locus_tag="PKN91_05480"
     CDS             5180..5317
                     /gene="rpmH"
                     /locus_tag="PKN91_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNS27560.1"
                     /translation="MSKRTYQPSKVRRARTHGFRARMATKNGQKVLARRRAKGRKKLI
                     P"
     gene            5317..5682
                     /gene="rnpA"
                     /locus_tag="PKN91_05485"
     CDS             5317..5682
                     /gene="rnpA"
                     /locus_tag="PKN91_05485"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNS27561.1"
                     /translation="MRLTFPPQRRLRTKPDFQKVHGAGKRIADHFFSMIVRPNELGAP
                     RLGLAVAIRTVGNAVARNRVRRIVKESFRLAQHELPAVDIVVAARAPVKGAPAADLRA
                     SLDVLWKKVARQCASSSRA"
     gene            5658..5882
                     /gene="yidD"
                     /locus_tag="PKN91_05490"
     CDS             5658..5882
                     /gene="yidD"
                     /locus_tag="PKN91_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006750110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNS27562.1"
                     /translation="MRVIIQGLIRLYQWTLSPLLGPKCRFYPSCSHYALEAVGRYGVL
                     RGGWLAAKRIGRCHPWHPGGIDPVPHLHDH"
     gene            5872..7599
                     /gene="yidC"
                     /locus_tag="PKN91_05495"
     CDS             5872..7599
                     /gene="yidC"
                     /locus_tag="PKN91_05495"
                     /inference="COORDINATES: protein motif:HMM:NF002352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNS27563.1"
                     /translation="MTTNPRVFLWLGLVLLCWLNYETWVRDYTPVPKTAEQVAQEIVE
                     RSAALEGNVPEAPPTAATPPVPVGGEVPAAEVPEMPLTGAAGEVAPAAGALAPAAAPT
                     VRVLTDVLDLEIGLAGGELRRADLLVYPLVKGKTQPVRLFNRDGPTGFYVMQTGLTGA
                     AGDVRPTHVETFTAPAGEFRLAEGERELRVPLTWTDGKGVTVTKTFVFERARYDIDLE
                     YSVRNDTDAPWSAAAYAQILRDNIPVDRSMFSPDSYAFKGPAIYDGTKYRKLKLKDAD
                     DLRLSLDVTGGWIAGMQHHFVTAVVPPVEQTSRISLKADGDQYLLSTVGATQTIAPRS
                     TGVLRDQLFVGPKLQAQLETTGPELERVADYGMLTPLAKPLFILLKWLHKLVNNWGWA
                     IVLATCVLKLLFYPLSEKAGKSMAKMKTLAPRIKNLQELYKDDREKLGRAMMKMYQDE
                     KINPVAGCLPILIQMPVFLAFYWVLLESVEMRQAPFMLWINDLSVRDPFFVLPAIMAA
                     AMFGQYKLNPAPPDPVQAKVFMVMPFAMSAMFAFFPAGLVLYWVTNTLLSIAQQWNIN
                     RRIEAQAKR"
     gene            complement(7615..8373)
                     /locus_tag="PKN91_05500"
     CDS             complement(7615..8373)
                     /locus_tag="PKN91_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS27564.1"
                     /translation="MTDQLRFRHVVYRNYRVWRKLLAASLVGNLLDPLFWLVGLGFGL
                     GSLLPSVGGMRYLEFLGSGMICYGVMYSTSFEGLWSAFTRLKQQRTWEGILHAPMTVT
                     DIVVGEWVWAALKGVLSGVAILTVMAALGLVRSPMALWALPVMLLTGLAFAGIALVIT
                     ALARSYDHFVYYFTLFITPMMLISGVFFPTAQLPRAVQLVAQVLPLSHATQLARGLVL
                     GRPIDNAFAHVAALALIAVVGVVLARALTARRLR"
     gene            complement(8366..9271)
                     /locus_tag="PKN91_05505"
     CDS             complement(8366..9271)
                     /locus_tag="PKN91_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS27565.1"
                     /translation="MLTVQSLRKKFGELEVVRGVSFEVSAGECYGLLGPNGAGKTTTL
                     RCCLGLTAPDSGTITLDSLPVPARAREARARIGVVPQFDNLDPDFTVRENLRMYGRYF
                     GLSRSEVARRVPWLLDYAGLADKADAQIEALSGGMKRRLTIARALVNDPRVLFLDEPT
                     TGLDPQARHVVWERLKRLLGEGRTIILTTHFMDEAQRLCDRVAIMDHGVFLAEAPPHE
                     LIEREIEPVVIEVYGEGVSAWFETHARRLATRAELSGDTAFCYCREPEALLATLADAP
                     ELRVLRRAANLEDVFLKLTGRDLRD"
     gene            9349..10602
                     /locus_tag="PKN91_05510"
     CDS             9349..10602
                     /locus_tag="PKN91_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNS27566.1"
                     /translation="MSRAFTGSIIAALVLGVLVGYLCHREVTDPATLANLAGYFGIVT
                     DAFLRLIKMIIGPLVFSTLVVGIANMGAGAAVGRVGVRTIGWFLFASVISLLVGILMV
                     LLLEPGTSLALPIPADGGEGGVQLAQPGLKEFVHSLVPRSIAEAMANNAILQIVVFSV
                     FFGIALGALGEHGRGLVEVIDQVAQVMLKVTAYVMWAAAPAVFAAVAATVTAEGLGIL
                     VTYGKFIGSYYIALVILWVILVALGVMVIGPRVRTLVSMVREPLLLAFSTASSEAAYP
                     KTLEMLQRFGISRRIASFVLPLGYSFNLDGSMMYCTFATLFIAQAYGIELTAGQLVVL
                     LLLLLVTSKGIAGVPRAALVVLAATLPHFGIPEAGLLLVLAVDQFLDMGRSATNVVGN
                     SIAAAVVAKWEGELAAAPAAADGLR"
     gene            10648..11148
                     /locus_tag="PKN91_05515"
     CDS             10648..11148
                     /locus_tag="PKN91_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNS27567.1"
                     /translation="MTYRLHINGQVHAIDVPGEMPLLWVLRDSLGLVGTKYGCGMALC
                     GACTVHVDGVPQRSCRLPVSSVGDSEITTIEHIGSTEIGARLQKAWLDTDVMQCGYCQ
                     AGQIMSAAAMLAVTPKPSHAEIRATMAGNICRCACYNRIENAIRIAAGLEPGGESHDK
                     EHTDVV"
     gene            11138..13369
                     /locus_tag="PKN91_05520"
     CDS             11138..13369
                     /locus_tag="PKN91_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNS27568.1"
                     /translation="MWSERRTFLKASLLAGGGLALEAFTAVPARALAAAGGAGALATA
                     AAHGELTAFVSIAPDGIVTITAKNPDMGQGTKTSLPMMVAEELDCDWAQVRIVQADAD
                     TARYGFQMAGGSMSTPTSWMPMRQTGAAARAMLLQAAAQQWSVPVAGLTTSSGRITHA
                     ASGRSCGYGEVASAAAQLAPPAAESVPLKQPGEFKIIGRAIAGIDGPRVLRGEPLFGI
                     DTRLPGMLYAVYEVAPAHGGRLVKANIEAAKRAPGVRHVVPIIGDGNADSGLADGVAI
                     VATHWWLANEARAKLDVQWDLARAKGHSTEAYRARASEALAKGGGHELRRDGDPDAAL
                     ASAATRVSARYDYPFVAHAALEPQNCTALFKDGKVEIWAPTQSPGPGRELTQKLLGLP
                     ALEDVTVHVMRSGGGFGRRLMNDYMLQAAAIAKALPGTPIQLIWSREDDIRRDYFRPA
                     GWHALEAGLDAEGRLIAFTDHFVTFGQGENTARGANFTLTMPPADLIPNLRFTQTTMP
                     TVLTTGWLRAPGSNALCFMSQGFLDEVAHAAGKDLPALMLELLGEPRVIPPFGGRGVP
                     FDTARARAVIEKVLETSQWRTGATGARGAAGAARAKGFAFYFCHRGYFAEVVDASVAD
                     RAVTVHKVWVAGDVGSHIINPFGAENQVRGSIIDGLAQALVGQAIDVVDGVVQQSNFH
                     DFPLARMPITPAIDIAWVHSDHPPTGLGEPSLPPVIPALTNAIYAATGTRIRSLPVKL
                     PAA"
     gene            13411..14766
                     /gene="mnmE"
                     /locus_tag="PKN91_05525"
     CDS             13411..14766
                     /gene="mnmE"
                     /locus_tag="PKN91_05525"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNS27569.1"
                     /translation="MTRADTIVATATPPGRGGVAVVRVSGPKAPEIAASMTASDLPVA
                     RRVTLARFLDAAGEPIDVGLLLFFAAPHSYTGEHVVEFHGHGGPVVSEALVRRAIELG
                     ARRARPGEFTQRAFLNDKIDLAQAEAVADLIDAGSREAARAAMRSLQGEFSAMVLGLV
                     DAVTELRAWVEAAIDFPEEEIDFLADRELDSRLGGVREHFAAVERSARQGRLLREGMT
                     VVIAGRPNAGKSSLLNRLAGYDAAIVTAIPGTTRDIVRERIDIDGMPLHVLDTAGLRD
                     APDVIEAEGIRRATAEMARADRVLFIVDASEDPDAHGFAEERARLPAEVPVTLVLNKI
                     DLATGIPMANTVSGPPRVAVSALTGAGLDVLRAHLRECMGYQIADAGTVSARARHLDA
                     LARAKAHVEEAGRQLREQRAGELVAEELRQAQAALGEITGEVTSEDLLGRIFSSFCIG
                     K"
     gene            14786..15817
                     /locus_tag="PKN91_05530"
     CDS             14786..15817
                     /locus_tag="PKN91_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNS27570.1"
                     /translation="MKAIRFIRRGRPPEVVDVRTPTPAAGQILVKIAGAGVCHSDLHI
                     LDEDLGLEGPFILGHENSGHVAALGEGVQGWKEGDPVLVYGPWGCGRCHTCQTSAENY
                     CENHASIGTMGGGLGADGGMAEYMLVPSPRLLVPLGKLDPVDAAPLADAALTPYHAIK
                     AALPLLTPEATVLVIGIGGLGHMAVQILKAMTPARLIAGDIDDSKLAHARELGVEHVV
                     NTRDGDAAAEAITQLAGPRGVTVALDIVGAQPTVDLAVRVVGRNSRLSVVGLAGGAVH
                     YVANSPPYGCEVSIPYWGSRTELMEVVALAAAGRIRAEVERFPLEQAVAVYQRLREGR
                     IRGRAVLQP"
     gene            15844..17679
                     /gene="recQ"
                     /locus_tag="PKN91_05535"
     CDS             15844..17679
                     /gene="recQ"
                     /locus_tag="PKN91_05535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNS27571.1"
                     /translation="MLRDVFGYPAFRPDQAEIITHVAAGGDALVLMPTGGGKSLCYQV
                     PALLREGVGVVVSPLIALMQDQVAALKEAGVRAEFLNSTLDWQQAQEIECRARAGELD
                     LLYLAPERLLTERGLALLDRLTIALFAIDEAHCVSQWGHDFRQEYLQLAVLADRYPSV
                     PRIALTATADEATRREMIERLRLQGARSFVQSFDRPNIRYRIAGKDSPRRQLLRFILD
                     EHAGQCGIVYCLSRKSVEETAQMLVDNGVDALAYHAGFSAAEREVRQTRFIEEDGVVI
                     VATIAFGMGIDKPDVRFVAHVDLPKSLEGYYQETGRAGRDGASAEAWMVYGLADVVQQ
                     RRLIDQSEAGEAHRRVAHAKLDAMLGLCETVRCRRQYLLEYFGEASRPCGNCDNCLEP
                     PAQFDGTEAAQKLLSCIYRCEQASGFAFGAQHIIDVLRGERTDKVLAHGHERISTFGI
                     GADRDPTQWRAVLRQLLTMRLVTVDHDHWNVLRLTDTSREVLAGARRLTLRQDAPRQG
                     ARKGEGRRRRKGSAAPPGAPTPPASGPASPLEETVFQALRAWRHDVARAHGVPAYTVL
                     HDASLREIARRRPESAESLAGISGIGAIKLARYGEAIIEVVRSRQ"
     gene            17714..18823
                     /locus_tag="PKN91_05540"
     CDS             17714..18823
                     /locus_tag="PKN91_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNS27572.1"
                     /translation="MKKRYRILGTLAIVLVVALAGGAFMLSRNAPCPAAPPSPTTSET
                     MKAVVSACYGPPAFLRVALVDRPTPADDRILVKVHAAAVNPLDWHYMRGEPYIMRMDT
                     GLGRPAESRLGVDFAGTVEAVGSKITRFKPGDEVFGGRTGAFAEYVVVSERSAVAAKP
                     AGTGFEQVAALPIAAITALQALRDKGELRAGEKVLINGASGGVGTFAVQLAKHFGAEV
                     TGVCSTRNVDLVRSIGADHVIDYTREDFTRGEQRYDVVIDNVGNHSLLAVRRVLAPDG
                     RYVIVGGPSEGKWLGVMMTPLKAYLLAPFVKQKVSFMLARLNAADLALLADLAEAGKL
                     TPVIDRRYSLDEVPEAIEYLETGRARGKVIIDIRK"
     gene            complement(18899..>19768)
                     /locus_tag="PKN91_05545"
     CDS             complement(18899..>19768)
                     /locus_tag="PKN91_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27573.1"
                     /translation="RFGKELQWLENRLPIDPRFRSAQLGALAPIRVVNVVFAAGDGNH
                     GVQTAAFNLPNDERVVAEKGSKRVMLRNFQQAKFDKVLQPIAAIALDAQDAELVAFEP
                     FFTHILMHELMHGLGPQTITVAGRATTVRQELKELNGTLEEAKADISGLWALQQLMDK
                     GVIDKRQERSMYLTFLASSFRTLRFGLNESHAKGMALQVNHLLDHGAIRVAADGRFGL
                     DLQRTKRAVTSLTRDIMTLQANGDYAGAKALLEREVTIRPEVQRVLDRLGAVPIDIAP
                     RFVTASELPRPGR"
BASE COUNT         3173 a         6657 c         6676 g         3262 t
ORIGIN      
        1 gagccgccgg ccgagtcgcc ctcgacgatg aagatctcgg acagcgccgg gtccttctcc
       61 tggcagtcgg ccagcttgcc gggcaggcct gcgatatcga gcgcgccctt cctgcgggtc
      121 atctcacgcg ccttgcgcgc cgcttcgcgc gcgcgcgccg cctcgatgat cttgccgacg
      181 atcgacttgg cgtcggccgg gtgctcgagc aggaactcgg acagtttgct cgccaccgcc
      241 gtctcgacga tgcccttcac ctccgaggac acgagcttgt ccttggtctg cgaggagaac
      301 ttcgggtccg ggagcttgac cgagaggatc gccgtgaggc cttcacgcgc gtcgtcgccg
      361 gtggtcggca ccttctcgcg cttggccacg ccctccctct cgatgtagtc gttcagcgtg
      421 cgggtgagag ccgctcgaaa gcccgacagg tgcgtgccgc cgtccttctg cggaatattg
      481 ttcgtgtagc agtacatcga ttcctggtag gaatcgttcc actggagagc cacttcgacc
      541 tgaatcggac cctcttgtgt ccgaaaccag aacactgtgt tgtggatcgg ggtacggctg
      601 cggttcagat gcgtcacgaa ggcctggagg ccgccctcgt gctggaacac gtcggacttg
      661 tcctcacgct cgtcgacgag ttcgatgcgg acacccgaat tgaggaacga cagctcgcgc
      721 aggcgcttgg cgaggatctc gtagttgaac gtgatgttcg tgaagatctt cgcgctcggc
      781 ttgaagcgga tggtggtgcc acgctcggtc gtctcgccga cgacggcgag cggcgcctgg
      841 ggctcgccca tccggtactc ctggcgatgg agcttgccgt cgcgggcgat ggtcatctgc
      901 aggtaatcgg acagcgcgtt gaccacggac acgccgacgc cgtgcagacc gcccgaaacc
      961 ttgtaggacg tgtcgtcgaa tttaccgccc gcgtggagca cggtcatgat gacttcggct
     1021 gccgaacggc cctcctccgg atggatatcc acaggaatcc cacggccgtt gtcggctacg
     1081 gtgatggact cgtcggcgtg gatcgtgacg acgacgcggg tgcaataacc cgccagcgct
     1141 tcgtcaaccg agttgtcgac cgcctcgaat accatgtggt gcagacccga gccatcatcg
     1201 gtgtcgccga tgtacatccc ggggcgcttg cgcaccgcgt cgagaccctt caagaccttg
     1261 attttgcttg aatcgtaaac gtctttttcg gtcatgtacc actcggtatc aggggtcccg
     1321 cattatagcg tttcggccct gccctgtccc atctggtaga cacgatcagg gttgccgaag
     1381 aggcttgaat cgggcgccag ggtcgtgacc acgagctgtg tctcgagcgc ccgaatgcgc
     1441 tcgatgacgg cgcccagccg aaagttatcc agttctgcgg ccgggtcgtc gatcagcagc
     1501 gtcggccgga tctgcaggct gtctcgcagc agctcgagct gggccagcac catcgacagc
     1561 gcaagcagct tctgctgtcc gcgggagagc acctcccggg ctgcgcgggt acccatccgc
     1621 aacctcagat cggcccggtg gggtccggcg ctcgtggttc cacgtgaaac atcgtgggcg
     1681 gcgccggccg ccagggcttc ttcaagggag cgctcccggt cccagccggt gctccaggtt
     1741 gctccgacgg tgaagcccag caacgcggtc tgcacccgca tccagacggg ctccagcgcc
     1801 gcatagtgcg ccgcccttgc ctccgccatg ggcacgcccg cagcgaccaa ttcgcggtcc
     1861 cagggcttga ggccctccgg gccgacgccg gcgcgcagcg cggcgttgcg ctgcttcagc
     1921 gcgcgggcgt agcgggccca gtgcaccccg aactgaggtt ccacgtggaa cacactccaa
     1981 tcgagccacc gccgtcggcc ggtcgctcca ccgtccacga gctgctggac gccgggttcg
     2041 atgacctgaa cggggaagac tccggtgagc tccgtgagcg cctggacgaa gtttccgtcc
     2101 accttggcac gtgtcacggc cgtggcatgc ccctgccctc gctgcacctc gactccgatc
     2161 gcgtgccgat ccgtgcgtcc gaatacgatc aggcgatcgg cgccatggcg gatgaggcgc
     2221 tccgagctcc gggtacggaa cgagcgccca cggcccagca gaaaaacagc ctcgagcaag
     2281 gaagtcttgc ccgtgccatt cgcaccggca atgagattga gcccagggtg gagcgtcagc
     2341 tccagtcgct cgatgcagcg cagatcctcg acgaggagat cgacgagggg catcgcgccg
     2401 tcagagacgc atcggcatta cgacatagcg agccgttgag gtggcgtccg cgcggatgag
     2461 gcagctgcta ttggcgtccc cgaggccgat ctgcactttg tctccgtcga tggcgcccaa
     2521 cgcatccagc aggtaattga cgttgaagcc gatctcgacc tcctcgccct ggtagctcac
     2581 ctcgatctga tcctccgcct cttcctgctc ggggttatgg gcctggatcg tcaacagatc
     2641 aggcttcacg gtcagccgca cgccgcggta cttctcgttc gagagaatcg cggttcgctg
     2701 caaggcctgc cgcagaggct cgcgatcggc ttcgaccacg cgtgacggcg aggccgggat
     2761 gacacggcca tattccggga atctgccgtc aattagcttc gatgtgaagc gaacgtcgcc
     2821 gatctgggcg cgaatgtgat tggtgccgat cgccagctcg atattgccct cggcaccgag
     2881 cagccgctgc agttcgagga cacccttgcg cggtacgatc acctgctgcg cggtgctgtt
     2941 cccgctctcg agcgggcagt cgcagatcgc gagccggtga ccatcggtcg cgaccgctct
     3001 caggttcttg ccatcggcct cgatcagcag gccgttgagg tagtagcgca catcctgctg
     3061 cgccatcgag aaatgggtct tctcgatcag tcggcggaac tccgcctggg gcacggtgag
     3121 ggtctgctgc gcgttgatct cttcgacgac cgggtattcg gccgacggca ggctcgagag
     3181 cgtgaagcgg ctgcggcccg cgcgcacgat cacccgctca ccctccgtcg ccagcgtgac
     3241 attcacgccg tccggcaagg cgcggaaaat gtcgagcagt ttgcggcccg gtacggtgat
     3301 gtcccctgcc tgctgcacgg tggcttcggt cgccgccacg agctccactt cgagatccgt
     3361 gcccgtgatg gagaggcgat tgccgcgtgc cgagagcagt acgttggcga ggataggcat
     3421 cgtctgccga cgttcgacga caccgatgac gctctggagc gggttcaata cctgggcgcg
     3481 ggtggccgaa agcttcatga gacgaacctt cctgaaatct tgattagtct tgtattagaa
     3541 ccagaagcag tagcagttag gcctgtggat gggcgaacaa agccgaaaat cctccgctgc
     3601 ccaatcactt gcccttcacc ccgtgcagcg caatcctgtt ggcgctgcgc ggtttccctg
     3661 tgcgtgaatt gtgcgcaact ttacgcacag tttcatccac aacaaataca ccgttaacca
     3721 gtcagggttc tcaacaggtt tgagtagtcc tcgctgatgc gctggtcgga atcgcggaat
     3781 tccttgatgc gcttgcaggc gtgcagcacg gtggtgtggt cgcggccgcc gaaggcatca
     3841 ccgatctcgg gcaggctgtg gctcgtgagc tccttggcga gcgccatggc aacctggcgt
     3901 ggtctcgcga tcgagcggtt gcggcgcttg gagagcaggt ccgcgacgcg caccttgtag
     3961 taatcggcca cggtcttctg gatgttctcg accgtgacca gcttggcctg cagcgacaac
     4021 aggtccttga gcgcttcctt cgcgaattcc agcgtgatcg gccggcccgt gaagcgcgaa
     4081 ttggcgatca cccggcgcag tgcgccctcg agttcgcgca cattcgaacg gatgcgtttg
     4141 gcaatgaaga acgccacttc ctcgggcagt gcgacccccg ccgcctggcc tttgctcatc
     4201 aggatcgcca cgcaggtctc gagctcggga ggctcgatcg cgaccgtgag gccccagccg
     4261 aaacgggact tgagccgctc ctcgagtccg gtgacctcct tcggatagcg atcacaggtc
     4321 acgatcacct gctgctggcc ttccagcagc gcgttgaaag tgtggaagaa ctcttcctgc
     4381 gaacggtcct tgccggcgaa gaactggata tcgtcgatca gcagcgcatc gagcgagcgg
     4441 taggctgcct tgaattcgtt catggtgttg tgctggagcg cacgcaccat gtcgctcacg
     4501 aagcgctcgg agtgcacgta ggcgatgcgc gcgtccggat tgcgctggcg gatcatgtgg
     4561 ccggccgcgt gcatcaggtg ggtcttgccg agaccgaccc cgccgtagac gaacagcggg
     4621 ttataggctt tgcccgggtt ttcccccacc tgcagtgctg cggcgcgggc gagctggttg
     4681 ctcttgccct cgacgaatgt ctcgaacgtg tagtccgggt tcaggcgcgc gccgactacc
     4741 aggccctcgg tccgccggcc ccggggcgtg gccgcgggct cgggggcgct cgccgttgcc
     4801 gaccggctgc tgcggctgcc gacctcgacc gtgacgatcg gggcatcccc ggtcgactcg
     4861 tcgcggagta gctcaccgat gcgcgcgagc aggttgccgt tgacccagtc gacgacgaag
     4921 cggttggggg ccagcagctt cagggcaccg tcgccctgga cggcctgcag cgggcgtacc
     4981 caggtattga actgctgttc aggcagttca tcctcgagag cgcgcaagca acgtgcccag
     5041 agcccagctt ccaatgacaa ccccgtgagc gtagagcctg ccaccggacg gggcagggcc
     5101 gcggagttta acagggccgg gcgtattgac actcccgccc ttcgtgctta cccttcccgc
     5161 cctttttggt gcgcccatca tgagcaaacg cacgtatcaa cccagcaaag tccgccgcgc
     5221 ccgtacgcac ggtttccgtg cgcgcatggc gaccaagaat ggccagaagg ttctcgcccg
     5281 gcgacgcgcg aagggccgca agaagctgat tccctgatac gtctcacttt cccgccgcaa
     5341 cgccggttgc gcaccaaacc ggacttccag aaggtccacg gcgcgggcaa acgtatcgca
     5401 gaccactttt tcagcatgat cgtcaggccg aacgaactcg gcgcaccgcg cctcggactc
     5461 gccgtcgcga tccgcaccgt cggcaacgcc gtggcacgca accgcgtgcg gcgcatcgtc
     5521 aaggaatcct ttcgcctcgc gcagcatgag ctgccggccg tcgacatcgt ggtcgcggcc
     5581 cgcgcgcccg tgaagggcgc gcccgccgcc gatctgcgcg cgagcctcga tgtgctctgg
     5641 aagaaggtcg cgagacaatg cgcgtcatca tccagggcct gatccgcctc taccagtgga
     5701 cgctgagccc gctgctcggg ccgaagtgtc gcttctatcc ctcgtgttcc cactacgcgc
     5761 tcgaggccgt gggccggtac ggtgtgttgc gtggcggctg gctcgccgcc aaacgcatcg
     5821 gccgctgcca cccctggcat ccgggcggca ttgatccggt accgcatctt catgaccact
     5881 aatccgcgcg tattcctctg gcttggcctg gttctcctct gctggctcaa ttacgagacc
     5941 tgggtgcgtg actacacgcc cgtgccgaag accgcggaac aggtcgcgca ggaaatcgtc
     6001 gagcgtagtg cggcgctcga gggcaatgtg cccgaggcgc cgccgaccgc cgcgacgcca
     6061 ccggtgcccg tcgggggtga ggtgcccgcc gccgaggtgc ccgagatgcc gctcacgggc
     6121 gcagccggcg aggtggcgcc cgcggccggt gcgctggcgc cggccgccgc accaaccgtg
     6181 cgcgtgctca ccgacgtgct cgacctggag atcggcctcg ccggtggcga gctgcggcgt
     6241 gccgacctgc tcgtctatcc gctcgtgaag ggcaagacgc agccggtgcg cctgttcaat
     6301 cgcgacggcc cgaccggctt ctacgtgatg cagacgggtc tcaccggcgc cgccggcgac
     6361 gtgcgaccga cccacgtcga aaccttcacg gcgccggccg gcgaattccg cctcgccgag
     6421 ggcgagcggg aactgcgcgt gccgctcacc tggaccgacg gcaagggtgt caccgtgacg
     6481 aagaccttcg tcttcgagcg cgctcgttac gacatcgacc tcgagtacag cgtcaggaac
     6541 gacaccgatg cgccgtggag cgcggccgcg tacgcgcaga tcctgcgcga caacatcccg
     6601 gtcgaccgct cgatgttttc gcctgacagc tatgcgttca agggcccggc gatctacgac
     6661 ggcaccaagt accgcaagct gaagctgaag gacgcggacg acctgcgcct gtcgctcgat
     6721 gtgaccggcg ggtggatcgc cggcatgcag caccacttcg tcaccgcggt ggtgccgccg
     6781 gtggagcaga cttcgcgcat ctcgctcaag gccgatggcg accagtacct gctctcgacg
     6841 gtgggtgcga cgcagacgat cgcgccgcgc agcaccgggg tgctgcgcga ccaactgttc
     6901 gtgggaccca agctgcaggc gcagctcgag acgaccggcc cggaactcga gcgcgtcgcc
     6961 gactacggca tgctgacgcc gctcgccaag ccgctgttca tcctgctcaa gtggctgcac
     7021 aagctggtca acaactgggg ctgggccatc gtgctcgcga cctgcgtact gaagctgctc
     7081 ttctacccgc tgtcggagaa ggccggcaag tcgatggcga agatgaagac gctcgccccg
     7141 cgcatcaaga acctgcagga gctctacaag gacgatcgcg agaagctcgg acgcgcgatg
     7201 atgaagatgt accaggacga gaagatcaac ccggtcgcgg gttgtctgcc gatcctgatc
     7261 cagatgccgg tgttcctcgc gttctactgg gtgctgctcg agagcgtcga aatgcgccag
     7321 gcgccgttca tgctgtggat caacgatctg tccgtgcgcg atcccttctt cgtgctgccg
     7381 gcgatcatgg ccgcggcaat gttcgggcag tacaagctga accccgcacc gcccgatccg
     7441 gtgcaggcga aggtcttcat ggtgatgccg ttcgcgatgt cggcgatgtt cgcgttcttc
     7501 ccggccggcc tcgtgctcta ctgggtcacg aacacgctgc tgtccatcgc gcagcagtgg
     7561 aacatcaacc ggcggatcga ggcgcaggcc aagaggtagg cccggaggcc cgcgtcaccg
     7621 caaccttcgc gccgtgagcg cgcgggcgag gacgacgccc acgacggcga tcagcgcgag
     7681 cgcggccacg tgggcgaagg cgttgtcgat cggccgcccg agaacgaggc cccgcgcgag
     7741 ctgcgtggca tgcgagagcg gcagcacctg cgcgacgagc tgcaccgcgc gcggcagctg
     7801 cgcggtcggg aagaacacgc ccgagatcag catcatcggc gtgatgaaca gtgtgaagta
     7861 gtagacgaag tgatcgtagc tgcgtgcgag cgccgtgatc accagcgcga tgccggcaaa
     7921 cgcgagtccg gtcagcagca tcaccggcag cgcccacagc gccatcggcg agcgcaccag
     7981 cccgagcgcg gccatcaccg tgaggatcgc gacgcccgac agcacgccct tcagcgcggc
     8041 ccacacccat tcgccgacga cgatgtcggt cacggtcatc ggcgcgtgca ggataccctc
     8101 ccaggtacgc tgctgcttga ggcgcgtgaa ggccgaccac aggccctcga agctggtgga
     8161 atacatcacg ccgtagcaga tcatcccgga gccgaggaac tcgaggtagc gcatgccgcc
     8221 gacactcggc aggaggctcc cgaggccgaa gccgagcccg accagccaga agagcgggtc
     8281 gagcaggttg ccgacgagcg atgccgccag cagctttcgc cacacgcggt aattgcggta
     8341 cacgacatgc cggaaacgca gctggtcagt cacgcagatc gcgccccgtg agcttcagga
     8401 acacatcctc gagattcgcc gcgcggcgca gcacgcgcag ctccggcgcg tccgcgagcg
     8461 tcgcgagcag cgcctcgggc tcgcggcagt agcagaacgc ggtgtcgccc gaaagctcgg
     8521 cgcgcgtcgc cagccggcgc gcatgcgtct caaaccaggc gctgacgcct tcgccgtaga
     8581 cctcgatcac caccggctcg atctcgcgct cgatcagttc gtgcggtggc gcttcggcga
     8641 ggaacacgcc gtggtccatg atcgcgacgc gatcgcagag ccgctgggcc tcgtccatga
     8701 agtgggtcgt caggatgatc gtgcggccct cgcccagcag gcgcttcagg cgctcccaga
     8761 cgacgtgccg cgcctgcggg tcgaggccgg tcgtcggctc gtcgaggaac agcacgcgcg
     8821 gatcgttgac gagcgcgcgc gcgatcgtca gccggcgctt catgccaccc gagagtgcct
     8881 cgatctgcgc atcggctttg tccgcgaggc ctgcgtagtc gagcaaccag ggcacgcgcc
     8941 gggcgacctc gctgcgcgac agaccgaagt aacgcccata catgcgcagg ttctcgcgca
     9001 ccgtgaaatc ggggtcgaga ttgtcgaact gcggtacgac gccgatacgc gcgcgcgctt
     9061 cccgagcacg cgccggcacc ggcagcgaat cgagcgtgat cgtgccggaa tcgggcgcgg
     9121 tgaggccgag gcagcaccgc aacgtggtcg tcttgccggc gccgttcggg ccgagcaggc
     9181 cgtagcattc gccggcagac acttcgaagc tgaccccgcg cacgacctcg agttcgccga
     9241 acttcttgcg tagcgactgg acggtgagca caggcccgca aggataccgg agcgcccttg
     9301 cggggggagc ggattgtcgc cagagtgacc ggcaaaaggg ggtcacccgt gtccagagcc
     9361 ttcaccgggt ccatcatcgc cgcgctcgtg ctcggcgtcc tcgtcggcta cctgtgccat
     9421 cgcgaagtca ccgatccggc cacactcgcg aacctcgccg gctacttcgg tatcgtcacc
     9481 gacgccttcc tgcgcctgat caagatgatc atcggcccgc tcgtcttctc gacgctcgtc
     9541 gtcggcatcg cgaacatggg cgccggtgcc gcggtcggcc gcgtgggcgt gcgcacgatc
     9601 ggctggttcc tgttcgcctc ggtgatctcg ctcctggtcg gcatcctgat ggtgctgctg
     9661 ctcgagcctg gcacgtcgct tgcgctgccg atcccggcgg acggcggcga aggtggcgtg
     9721 cagctcgcgc aacccgggct caaggagttc gtgcacagcc tcgtgccgcg ctcgatcgcc
     9781 gaggcgatgg ccaacaatgc gattctgcag atcgtcgtgt tctcggtgtt cttcggcatc
     9841 gcactcgggg cgctcggcga gcacgggcgc gggctcgtcg aggtgatcga ccaggttgca
     9901 caggtgatgc tcaaggtcac tgcctatgtg atgtgggcgg cggcgccggc ggtcttcgcc
     9961 gcggtggcgg cgaccgtcac cgccgagggg cttggcatcc tcgtcaccta cggcaagttc
    10021 atcggcagct attacatcgc gctcgtgatc ctgtgggtga tcctcgttgc gctcggcgtg
    10081 atggtgatcg gtccgcgagt gcgcacgctg gtctcgatgg tgcgtgaacc gctgctgctc
    10141 gcgttctcga ccgcgagctc cgaggcggcc tatccgaaga cgctcgagat gctgcagcgc
    10201 ttcggcattt cgcggcgcat cgcgagcttc gtgctgccgc tcggctactc gttcaacctc
    10261 gacggctcga tgatgtactg caccttcgcc acgctgttca tcgcgcaggc ctacggcatc
    10321 gagctcaccg ccggccagct cgtcgtgctg ctgctcctgc tgctggtcac cagcaagggc
    10381 atcgccggcg tgccgcgcgc ggcgctggtc gtgctggcgg cgaccctgcc gcatttcgga
    10441 atcccggaag ccggcctgct gctggtactc gcggtcgacc agttcctcga tatgggccgc
    10501 agcgcgacca acgtggtcgg caactcgatc gcggcggccg tggtcgccaa atgggagggc
    10561 gagctcgcgg ccgcaccggc tgccgcagac ggcttgcgct gaagccgtcc tggggctatc
    10621 taggagatct tccggacgga gcgatccatg acctaccgac tccatatcaa cggccaggtg
    10681 catgccatcg acgtgccggg cgaaatgccg ctgctctggg tgctgcgcga cagcctcggg
    10741 ctcgtcggca cgaagtacgg ctgcggcatg gcgctgtgcg gcgcctgcac cgtgcatgtc
    10801 gacggggtgc cccagcgctc gtgccgcctg ccggtctcga gtgtcggcga ctcggagatc
    10861 acgacgatcg agcacatcgg cagcaccgaa atcggcgcgc gattgcagaa ggcctggctc
    10921 gataccgacg tgatgcagtg cggctactgc caggccgggc agatcatgag cgccgcggcg
    10981 atgctcgcgg tgacgccgaa gccgagtcac gcggagatcc gcgccacgat ggccggcaat
    11041 atctgccgct gcgcctgcta caaccgcatc gagaacgcga tccgcatcgc ggccggcctc
    11101 gaaccgggcg gcgagtcgca cgacaaggag cacaccgatg tggtctgagc ggcgcacttt
    11161 cctcaaggcc tcgctgcttg ccggtggcgg gctcgcgctc gaagcgttca ccgccgtgcc
    11221 ggcgcgggca ctggcggccg ctggcggggc tggcgcgctg gccaccgccg ccgcgcatgg
    11281 cgagctcacg gcattcgtgt cgattgcgcc cgacggtatc gtgacgatca ccgccaagaa
    11341 ccccgacatg ggccagggca ccaagacatc gctgccgatg atggtcgccg aagagctcga
    11401 ctgcgactgg gcgcaggtac gcatcgtgca ggcggatgcg gatacggcac gctatggctt
    11461 ccagatggcc ggcggcagca tgtcgacgcc gacgagctgg atgccgatgc gccagaccgg
    11521 agcggccgcg cgcgcgatgc tgctgcaggc ggcggcgcag caatggtcgg tgcccgtggc
    11581 cgggctcacc acctccagtg gccgcatcac gcacgccgcg agcggccgct cctgcggcta
    11641 cggcgaggtg gcgagcgcgg ctgcgcagct cgccccaccc gcggccgagt cggtgcccct
    11701 caagcagccg ggtgaattca agatcatcgg ccgcgcgatc gcgggcattg acggcccgcg
    11761 cgtgctgcgc ggcgagccgc tgttcggcat cgacacgcgc ctgccgggca tgctctacgc
    11821 ggtgtatgaa gtcgcgcccg cgcacggcgg gcggctcgtg aaggcgaaca tcgaggccgc
    11881 gaaacgtgcg cccggcgtgc ggcatgtcgt gccgatcatc ggcgatggca acgccgacag
    11941 tggtctcgcc gacggcgtcg cgatcgtcgc gactcactgg tggctcgcga acgaggcgcg
    12001 cgcgaagctc gatgtgcaat gggatctcgc gcgcgccaag ggccacagca ccgaggcgta
    12061 tcgcgcgcgg gcctccgagg cgcttgcgaa gggcggtggc cacgaactgc ggcgcgacgg
    12121 cgatcccgat gccgcgcttg ccagcgcggc gacgcgcgtg tccgcgcgct atgactatcc
    12181 cttcgtcgcg catgccgcgc tcgagccgca gaattgcacg gcgctcttca aggacggcaa
    12241 agtcgagatc tgggcaccga cccagtcgcc cggaccgggt cgcgagctca cgcagaaact
    12301 gctcggtttg cccgcgctcg aggatgtcac cgtgcacgtc atgcgctccg gcggcggttt
    12361 cgggcggcgc ctgatgaacg actacatgct gcaggccgcc gcgatcgcca aggccctgcc
    12421 cggcacgccg atccagctga tctggtcgcg cgaggacgat atccgccgcg actacttccg
    12481 cccggcaggc tggcacgccc tcgaggccgg cctcgacgca gaagggcgat tgatcgcctt
    12541 caccgaccat ttcgtgactt tcggccaggg cgaaaacacg gcacgcggcg ccaatttcac
    12601 cctcacgatg ccgccggcgg atctcatccc gaacctgcgt ttcacgcaga cgacgatgcc
    12661 gaccgtgctg accaccggct ggctgcgcgc gcccggatcg aatgcgctgt gcttcatgtc
    12721 gcagggcttc ctcgacgaag tcgcgcacgc cgcgggcaag gacctgccag cgctgatgct
    12781 ggagctgctc ggcgagccgc gcgtgatccc gcctttcggc gggcgcggcg tgccgttcga
    12841 caccgcgcgg gcgcgcgccg tgatcgagaa ggtgctcgag acttcgcaat ggcgcaccgg
    12901 cgcgaccggt gcgcggggcg ccgccggcgc ggcgcgcgca aaaggctttg cgttctactt
    12961 ctgccatcgc ggctatttcg ccgaggtggt cgatgcgagc gtcgcggacc gcgcggtgac
    13021 cgtccacaag gtctgggtcg cgggcgacgt cggcagccac atcatcaatc cgttcggagc
    13081 ggaaaaccag gtgcgcggct cgatcatcga tggtcttgcg caggcgctgg tgggtcaggc
    13141 gatcgacgtc gtcgacggtg tcgtgcagca gagcaatttc cacgactttc cgctggcacg
    13201 catgccgatc accccggcga tcgacatcgc ctgggtgcac tccgaccacc cgccgacggg
    13261 cctcggcgag ccgtcgctgc cgccggtgat ccccgcgctc acgaatgcga tctacgcggc
    13321 caccggcacg cgcatccgct cgttgccggt gaagctgccg gcggcatagc gcggcggctc
    13381 catcgttccg ccacccacgg tcggatcgtc atgacacgcg ccgacacgat cgtcgccacc
    13441 gccacgccgc cgggacgcgg cggcgtggcg gtagtgcgcg tgtcgggccc caaggctccg
    13501 gagatcgccg cgtcgatgac cgcctccgat ctgccggtcg cgcggcgcgt cacgctcgcg
    13561 cgttttctcg acgcggccgg tgagccgatc gatgtcggcc tcctgctgtt cttcgctgcg
    13621 ccacattcct acaccggcga gcacgtggtc gagtttcacg gccacggcgg accggtggtc
    13681 tccgaggcgc tggtgcggcg cgcgatcgag cttggcgcac gccgcgcccg gcccggcgag
    13741 ttcacgcagc gcgcattcct caacgacaag atcgatctcg cgcaggccga agcggtcgcc
    13801 gacctgatcg atgcgggctc gcgcgaggct gcgcgcgccg cgatgcgctc gctgcagggc
    13861 gagttctcgg cgatggtgct cggactcgtc gatgcggtga ccgaactgcg cgcctgggtg
    13921 gaggcggcca tcgatttccc ggaagaggag atcgacttcc tcgccgaccg cgagctcgac
    13981 agccgtctgg ggggcgtgcg cgagcatttc gcggcggtgg agcgcagcgc gcgccagggg
    14041 cgcctgctgc gcgaaggcat gaccgtggtg atcgccggtc ggccgaacgc cggcaagtcg
    14101 agcctgctca atcgcctcgc cggctacgac gccgcgatcg tcaccgcgat ccccggcacc
    14161 acgcgcgata tcgtgcgcga gcgcatcgat atcgacggca tgccgctgca cgtgctcgac
    14221 accgcgggcc tgcgcgatgc ccccgatgtg atcgaggccg agggcatccg tcgcgccacc
    14281 gccgaaatgg cgcgcgccga tcgagtgctg ttcatcgtcg atgcctccga ggacccggac
    14341 gcacacgggt tcgccgaaga gcgcgcccgc ctgccggccg aggtaccggt gacgctcgta
    14401 ctcaacaaga tcgacctcgc gaccggcatc ccgatggcga acacggtcag cggcccgccc
    14461 cgcgtcgcgg tgtcggcgct gaccggcgcg gggctcgatg tgctgcgtgc gcacctgcgc
    14521 gagtgcatgg gataccagat cgcagatgcc gggaccgtgt cggcgcgcgc gcggcatctc
    14581 gacgcgctcg cgcgcgcgaa ggcgcacgtc gaagaggccg ggcgccagct gcgcgagcag
    14641 cgcgcggggg aactggtggc cgaggagctg cgccaggcgc aggcggcact cggcgagatc
    14701 accggagagg tgacgagcga ggacttgctg ggcagaatct tctccagctt ctgcatcgga
    14761 aagtgaacgg gagccaggag tttcaatgaa agccatccgc ttcatccggc gcgggcgccc
    14821 acccgaggtc gtcgacgtca ggacgccgac gccggcggcc ggccagatcc tcgtgaagat
    14881 cgccggcgcc ggcgtgtgtc attccgatct ccacatcctc gacgaggatc tcggcctcga
    14941 ggggccgttc atcctcggcc acgagaactc gggccacgtc gctgcgctgg gcgagggtgt
    15001 ccagggctgg aaggaaggcg atccggtcct cgtctacggt ccgtggggat gcggccgttg
    15061 ccacacctgc cagacctcgg cagagaacta ctgcgagaac cacgcgagca tcggcacgat
    15121 gggcggcggg ctcggtgccg atggcggcat ggcggagtac atgctcgtgc cttcgccgcg
    15181 gctgctcgtg ccgctcggca agctcgatcc ggtcgacgca gctcccctcg cggatgccgc
    15241 gctgacgcct tatcacgcga tcaaggccgc gctaccgttg ttgacgcccg aggccacggt
    15301 actggtgatc ggcatcggag ggctcggtca catggcggtg cagatactga aggcgatgac
    15361 gccggcgcgg ctgatcgccg gcgatatcga cgacagcaaa ctcgcgcatg cccgcgagct
    15421 cggtgtcgag catgtcgtca atactcgcga cggcgatgcc gcggcggagg cgatcacgca
    15481 gctcgccgga ccgcgcggcg tgaccgtcgc gctggacatc gtcggcgcgc agcccaccgt
    15541 cgatcttgca gtccgcgtcg tcggtcgcaa cagccgcctg agcgtggtgg gccttgcggg
    15601 cggcgccgtg cactacgtgg ccaacagtcc gccctacggc tgcgaagtca gcattccgta
    15661 ctggggttcg cgcaccgaat tgatggaggt cgtcgcgctg gccgccgccg gtcgcatccg
    15721 cgccgaagtc gagcgcttcc cgctcgagca ggcggtagcg gtgtaccagc gcctgcgcga
    15781 gggccggatc cgcggacgcg cggtgctgca gccctgagcg aactgctcgc gcgcgcgacg
    15841 cgcatcctcc gggacgtctt cggctatccg gcattccgcc ccgaccaggc ggagatcatc
    15901 acgcacgtcg ccgccggcgg ggacgctctc gtgctgatgc cgaccggcgg cggcaagtcg
    15961 ctgtgctacc aggtgcccgc gctgctgcgc gaaggcgtgg gcgtcgtcgt ctcgccgctg
    16021 atcgcgctga tgcaggacca ggttgcggcc ttgaaggagg cgggcgtgcg cgccgagttc
    16081 ctgaactcga cgctcgactg gcagcaggcg caggagatcg agtgccgcgc gcgcgccggc
    16141 gaactggacc tgctgtacct cgcgccggag cgcctgctga cggagcgcgg cctcgcgctg
    16201 ctcgatcgcc tcacgatcgc gctgttcgcg atcgacgagg cccattgcgt ctcgcaatgg
    16261 gggcatgatt tccgccagga gtacctgcag ctcgcggtgc tcgccgatcg ctatccgtcc
    16321 gtgccgcgca tcgcattgac cgcgacggcc gacgaggcga cgcgccgcga gatgatcgag
    16381 cgcctgcgcc tgcagggtgc gcgctcattc gtgcagagct tcgatcggcc caatatccgc
    16441 taccgcatcg ccggcaagga ttccccgcgg cgccagttgc tgcgcttcat cctcgatgag
    16501 cacgccggac agtgcggcat cgtctattgc ctgtcacgca aatcggtgga ggagacagcg
    16561 cagatgctgg tcgacaacgg cgtcgacgca ctcgcctatc acgccggctt cagcgcagcc
    16621 gagcgggaag tccggcagac gcgcttcatc gaagaggacg gcgtcgtcat cgtcgcgacg
    16681 atcgccttcg gcatgggcat cgacaaaccg gacgtgcgtt tcgtcgcaca cgtcgatctg
    16741 ccgaagagcc tcgagggcta ctaccaggaa accggccgcg cgggccgcga cggcgcgagc
    16801 gctgaggcgt ggatggtcta tggcctcgcg gacgtcgtgc agcaacgccg cctcatcgat
    16861 cagtccgagg cgggtgaggc gcaccggcgg gtcgcccatg caaagctcga cgcgatgctc
    16921 ggcctctgcg aaacggtgcg ctgtcgcagg cagtacctgc tcgagtactt cggcgaagcg
    16981 agccggccgt gcggcaactg cgacaactgc ctcgagccgc cggcgcagtt cgacggcacc
    17041 gaggccgcgc agaagctgct ctcgtgcatc taccgctgcg agcaggcgag cggcttcgcg
    17101 ttcggcgcgc agcacatcat cgatgtcctg cggggcgagc ggaccgacaa ggtgctcgcg
    17161 cacgggcacg agcggatcag cacgttcggc atcggcgcgg atcgggaccc gacgcaatgg
    17221 cgcgcggtgc tgcggcaact gctgacgatg cggctggtga cggtcgatca cgatcactgg
    17281 aacgtgctgc gcctcaccga cacgagccgc gaggtgcttg cgggtgcacg ccggctgacc
    17341 ctgcgtcagg acgcgccgcg ccagggcgcg cgcaagggcg aaggacggcg ccggcgcaaa
    17401 ggcagcgcgg cgccgccggg cgcgccgacg ccgccggcga gcggcccggc ttcgccgctg
    17461 gaggaaaccg tattccaggc gctgcgcgca tggcggcacg acgtggcccg cgcgcacggc
    17521 gtgccggcct ataccgtgct gcacgatgcg agcctgcgcg agattgcccg ccgccgcccg
    17581 gagtccgccg agtcgctggc ggggatctcg ggcatcggcg cgatcaagct cgcgcgctat
    17641 ggggaggcga tcatcgaggt cgtccgaagc cggcagtagt attggtgacc tctgaggcca
    17701 gggacaggct gccatgaaga agcgctacag gatcctcggt acgctcgcga tcgtgctcgt
    17761 cgtggcgctc gcgggcggcg ctttcatgtt gagccgcaac gccccctgtc ctgcggcacc
    17821 accatcacct acgacttcgg aaacgatgaa ggcggtggtg tcggcctgct acgggccgcc
    17881 cgcgttcttg cgcgttgcgc tcgtcgacag gccgactccc gccgacgacc ggattctggt
    17941 gaaggtgcac gcggccgccg tcaatccgct cgactggcat tacatgcggg gcgaaccgta
    18001 catcatgcgc atggacacgg gtctcggcag acccgccgag tcgaggctgg gggtcgattt
    18061 cgccggcacg gtcgaagccg taggcagcaa gatcacgcga ttcaagccgg gcgatgaagt
    18121 attcggcggc cgcaccggcg cgttcgccga gtacgtcgtc gtatccgagc gcagcgccgt
    18181 agcggcgaag ccggccggca ccggtttcga gcaggttgcc gcgctgccga tcgcggcgat
    18241 cacggcactg caggcgctgc gcgacaaagg cgagctccga gcaggcgaaa aagtcctgat
    18301 caacggcgca tcgggcggcg tcggcacttt cgccgtgcag ctcgccaaac acttcggcgc
    18361 cgaagtgact ggcgtgtgca gcacacgcaa tgtcgatctg gtgcgctcga tcggcgccga
    18421 tcacgtcatc gactacacgc gcgaagattt cacgcgtggc gagcaacgct acgatgtcgt
    18481 catcgacaat gtcggcaatc actcgctgct cgccgtgcgg cgcgtccttg cacccgatgg
    18541 ccgctatgtg atcgtcggcg gtccaagcga aggcaaatgg ctcggtgtca tgatgactcc
    18601 cctcaaggca tacctgctgg cgccgttcgt gaagcagaaa gtgagcttca tgctcgcccg
    18661 gctcaacgcc gcggacctcg cactgctcgc cgacctggcc gaggccggca agctcacgcc
    18721 ggtgatcgac cggcgctaca gcctggacga ggtgcccgag gcgatcgagt acctcgagac
    18781 ggggcgcgct cgcggcaagg tgatcatcga catcagaaaa tgaaccacag cgccggcttg
    18841 ggcccggcgt agagcaggtg tatcgcgtgc agcacggcga acccccggaa cggcggcttc
    18901 accggccggg ccgcggcagc tcgctcgcgg tcacgaagcg cggcgcgatg tcgatcggca
    18961 cggcacccag ccgatccagc acgcgctgca cttcgggtcg gatcgtgact tcgcgctcga
    19021 gcagcgcctt ggcgccggcg taatcgccgt tcgcctgcag cgtcatgatg tcgcgagtga
    19081 ggctggtgac cgcccgcttc gtcctctgca ggtcgaggcc gaagcggccg tcggcagcga
    19141 cgcggatcgc gccgtggtcg agcaggtggt tgacctgcag cgccatgccc ttcgcatgcg
    19201 actcgttcag gccgaagcgc agcgtgcgaa acgaggaggc aaggaaggtc aggtacatgc
    19261 tgcgttcctg gcgcttgtcg atcacgcctt tgtccatcag ttgctgcagc gcccagaggc
    19321 ccgagatgtc ggccttggct tcttcgagcg tgccgttcag ctccttcagc tcctgccgca
    19381 ccgtcgtcgc gcgacccgcg accgtgatcg tctgcggccc gagcccgtgc atcagctcat
    19441 gcatcaggat gtgcgtgaag aacggctcga acgcgacgag ttctgcgtct tgcgcatcga
    19501 gcgcgatcgc ggcgatgggc tgcagcacct tgtcgaactt ggcctgctga aaattgcgca
    19561 gcatcacgcg cttcgatccc ttttcggcga ccactcgctc atcgttcggc aggttgaagg
    19621 cggcggtctg cacgccatgg ttgccgtcgc cggcggcgaa gacgacgttc accacccgga
    19681 tcggcgcgag cgcgccaagc tgggcgctgc ggaagcgggg atcgatcggc agccggttct
    19741 ccagccactg cagttccttg ccgaagcg
//
LOCUS       DAOQVY010000040        19602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_3232_length_19602_cov_5.390137, whole genome shotgun sequence.
ACCESSION   DAOQVY010000040 DAOQVY010000000
VERSION     DAOQVY010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19602
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3232_length_19602_cov_5.390137"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="PKN91_05550"
     CDS             <1..1403
                     /locus_tag="PKN91_05550"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNS27574.1"
                     /translation="GDNVGDCAGMAADLFETYAVTLVATMLLGGLMLQDQGAAAVTYP
                     LALGAASIIASIIGTFFVKTSDGGKIMNALYKGVIVSGVIAAIAFWFITDSLMPEDAG
                     AMFGCALIGLVLTAAMIVITEYYTATEYSPVRKVAGASQTGHATNIIAGLGVSMKSTA
                     LPVLAVCFAIYGAYELGGLYGIAIAATSMLSLTGMVVALDAYGPITDNAGGIAEMAEL
                     PKKIRDITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHNLETHLLATGREMIE
                     FSLSDPAVIIGLFIGGLVPYLFGAMAMEAVGRAAGSVVNEVRRQFREIAGIMEGRAKP
                     DYSKAVDLLTRAAIKEMIVPSLLPILVPVIVAFGLNALMGPGAGLRALGGLLIGTIVT
                     GLFVAISMCTGGGAWDNAKKYIEEGNFGGKGSEAHKASVTGDTVGDPYKDTAGPAINP
                     LIKIINIVALLLVPLL"
     gene            1424..1681
                     /locus_tag="PKN91_05555"
     CDS             1424..1681
                     /locus_tag="PKN91_05555"
                     /inference="COORDINATES: protein motif:HMM:NF021559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HNS27575.1"
                     /translation="MTTKRMILAAALGSLVSLGTAVAADEKAETEKCYGVAEAGKNDC
                     HTASHACAGLATTDKDPAEWVRVPKGTCEKMGGKLEAPRPI"
     gene            1747..2568
                     /locus_tag="PKN91_05560"
     CDS             1747..2568
                     /locus_tag="PKN91_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HNS27576.1"
                     /translation="MPPSTPTRCGIGLRAAHHREVLRTAPDVGWFEAHSENYFADGGG
                     ALDTLLRVRERWPVALHGVGLGLGSTAPLDREHLRRLARLAARIEPWAVSEHVCWGQA
                     DGRHTNDLLPMPRTGESLRHLAARVAELQDALGRQVLLENVSSYLEFTGAEFTEAGYL
                     AALVAESGCALLLDVNNVYVNACNHGFDAEAYFDELPVDAVREIHLAGHEPIRIAGRV
                     IRIDTHDRPVCDEVWALCASARRRFGRVPTLIEWDADLPPLGVLVAEAQRADALG"
     gene            2555..3319
                     /locus_tag="PKN91_05565"
     CDS             2555..3319
                     /locus_tag="PKN91_05565"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HNS27577.1"
                     /translation="MPLDELQRRFVASLYGEAADLDGAISGAGGISATECLAIYRGNL
                     HVAFAKALELGFPVLAALCGPAFFTVLAREFQRAHPSASGDLHHIGGPLPHYLRQRYA
                     GSEFAYFADVAALEWAREESARAADAAPLDLRGLSAVADDAAASVRFALHPGVRLVAS
                     RWPVLAIWEAHQDPGGVRGVDLGAGPEQVLLRRAASGLVLERISAAEFDLLTTLGRGE
                     PLGAAFDAAIAADSTFDVGAALRRFVAGNVFASMGD"
     gene            complement(3316..4056)
                     /locus_tag="PKN91_05570"
     CDS             complement(3316..4056)
                     /locus_tag="PKN91_05570"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HNS27578.1"
                     /translation="MSTLVALQTALQQRVLCGDPSIEGSVAGDAREVATRLGIYEYAY
                     RARLTEALGVTYPVLRKLLGVAAFEAAAATYIAGAVPTHASIRWYGADLHRHLRGMHA
                     DLARWEWLLAEVFDAGDDFAMTEADIARVAPADWPALRLRWHRTVRRFEATTNAVECW
                     QAVSEDRPCPEPRATAPVSWVVWRRDPAIRFRSTTPAEGRALARLAGGASFAALCAGL
                     AQETGERDAARCAATMLKGWIGEGLLGR"
     gene            complement(4053..4583)
                     /locus_tag="PKN91_05575"
     CDS             complement(4053..4583)
                     /locus_tag="PKN91_05575"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HNS27579.1"
                     /translation="MDGVNLHDLLPLPHTAEAVAHVAARVRQVQDCLGRQILLENISS
                     YVNFTGAEMTEWEFVTSVCEAADCLLLLDVNNVYVNSVNHGFDAARYLRAIPVERVRQ
                     LHLAGHSRQGRFLVDTHDAPVDEAVWRLYEIAAARFPHAATMIERDDRIPPLEALVAE
                     LDRARAIAASALGAAA"
     gene            complement(4694..4882)
                     /locus_tag="PKN91_05580"
                     /pseudo
     CDS             complement(4694..4882)
                     /locus_tag="PKN91_05580"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
     gene            complement(4922..5188)
                     /locus_tag="PKN91_05585"
     CDS             complement(4922..5188)
                     /locus_tag="PKN91_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27580.1"
                     /translation="MIDSVKSNGAAIATAAALLFGALALPASAADEAKVHCMGVNSCK
                     GQSACKTANNACKGQNSCKGKGFLELTQPQCDAAKAEAGAKDPM"
     gene            5309..5803
                     /locus_tag="PKN91_05590"
     CDS             5309..5803
                     /locus_tag="PKN91_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNS27581.1"
                     /translation="MARSNFRERKFEELCAPLKADLLRYAFWLCRDRSVAEDIVQEAL
                     LRAWKSIDGLESAAAVKPWLLTIVRREFARTFERKRHETVDIAALDACQDIALASPEE
                     DPEINAMRQAMLRLEPIYREPLVLQVLFGYTTAEIAAHLAISVPAVLTRLYRAREKLR
                     GVLA"
     gene            5800..6495
                     /locus_tag="PKN91_05595"
     CDS             5800..6495
                     /locus_tag="PKN91_05595"
                     /inference="COORDINATES: protein motif:HMM:NF023287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3379 family protein"
                     /protein_id="HNS27582.1"
                     /translation="MNCEEFRAATGADPHATRADLAAHAAGCEACAAWRRELLRMDDV
                     IRRALAFDVGAERTGGPALPARSAWRPLSWRGWAMAAGVACATLVAGLLWLAPARTAL
                     ADAVVAHMSEEPGAWARTDSPADPVLLARVLARAGVRLNAGAGVASYANSCWFRGHHV
                     PHLVFQTEDGPVTVMVLPAERVAAPIEFDEQGYRGVLVPAEHGALAILSRGAMPSREL
                     TARLQAAFTYSAK"
     gene            6573..6827
                     /locus_tag="PKN91_05600"
     CDS             6573..6827
                     /locus_tag="PKN91_05600"
                     /inference="COORDINATES: protein motif:HMM:NF021559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HNS27583.1"
                     /translation="MTRNIVLASALGSLLALNLATASAAQAATEKCYGIAMAGKNDCA
                     TAAHACAGQSKTDRDPREWQLVPKGTCEKMGGRLAPTSPQ"
     gene            6824..7300
                     /locus_tag="PKN91_05605"
     CDS             6824..7300
                     /locus_tag="PKN91_05605"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNS27584.1"
                     /translation="MSVSAAYAAANRALASVLDRLRTPLLLACRLYVSWQFLKSGWLK
                     LGDWDTTLWLFREEYHVPLLPPEWAAVAGTVGETLFALSLIAGFASRPAALGLFAVNV
                     LAVISYRHVLLAPGYEAALAQHVLWGFMLLVLIAFGPGRVSVDQAAIGNGVGRAIA"
     gene            complement(7258..9591)
                     /locus_tag="PKN91_05610"
     CDS             complement(7258..9591)
                     /locus_tag="PKN91_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNS27585.1"
                     /translation="MSIVGLVNVLLGLVAILLLVLYLLQRRELKAVSALSQQVQRIAI
                     GGRLTGRVDLDTDQPELAALGTAINHLLGRVAAQGDHERLSPALFADLGDRIHEVVLV
                     HRDVILYANCQFASLVGADRAVLIGRRLADIVAADSAELVEQNLRRKLAGEATADRYE
                     IDIIGLQGVASRLEISTTIIDYDGGPAVLVTGVEVVPTQTVPSLRAPDLAIAAGEGGG
                     PAPRSEREDALESLAEGIVVTDAHGSIEYMNAAAEALAGVSRLEAQGRHFEDVVGLVD
                     EVERRAIADPIRHAIDSGSSVHAPRRALLVSHETGEERAIEPLTSPLRDAAGKVTGAV
                     VLLHDVTELRGMARQMSYQATHDALTGLVNRREFERRLGEAIEAARLGDGTHILCYLD
                     LDRFKLVNDTSGHVAGDSLLREVAKILRDSVRDSDTVARIGGDEFGMLLLGCPLDKAR
                     QIADDVCRAVAEHRFVWRDRLFNIGISIGIVELSHESGTMEEVLIAADSACYVAKRQG
                     SGQVAVYSARDEVLARQSGELQWLHRLQGALKENRFQLYHQPIVPALGTNGSGPALEV
                     LVRLRDTAGNDVAPGAFMHAAERYRLTTLIDRWVVQTTLAALGRGAIQTPVGRSVAIN
                     ISGQTLNDSQFLEFVVDCFDRTGVAPSRVCFEISEAAVSENLEQARRFIEVLHSMGCQ
                     FALDDFGAGAGSFVNLKTLPVDFVKIDGSFMKNFGTDLVNQAMVGAMIKLARTLDFRI
                     IAEAVEDNAALEAARTMGFDYLQGYAIARPTPLPMAA"
     gene            9801..10451
                     /locus_tag="PKN91_05615"
     CDS             9801..10451
                     /locus_tag="PKN91_05615"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HNS27586.1"
                     /translation="MSTEFALDQMVTQQVRAWDVLDERVLDVMRRVPRERYVPHAYRE
                     VAYADTAIPLAHGQRMLAPKVIGRILQAVEASARDAVLEIGTGSGYLTACLAASARVV
                     HSIDIYDDIAAAARERLAANGVANAEVRTADAFAEQIGTRYNVIVLTGSLPCYDERFQ
                     EALEAGGRLFAIVGEDQAMSARLVRRVSASSWTSEDLFETWVEPLVHAPRPPRFVF"
     gene            10573..12003
                     /locus_tag="PKN91_05620"
     CDS             10573..12003
                     /locus_tag="PKN91_05620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HNS27587.1"
                     /translation="MFSQAVLAEDLLTVYQRALAADPLIREADATRLAVRESKPQALA
                     ALLPQISGTAGYDKNKFEGTSDQAQVVSEGAAAGSITILSTSGTREPETETWSLDLRQ
                     SVFRWENWVALRRADKQVAQAEADFAAAEQDLILRVSTAYFDVLAARDTLEAQQANLQ
                     AVSRQLDQAEKRFEVGLIAITDVQESRAARDNAAAAVIAAKRQLATSEELLREVTGEK
                     YSTLDKPGDDLPLKSPQPNLEDDWVRVAMDQNLALVSSRLAADIARDDVRTALGGHLP
                     TIDLVASYGNQKTTTEQTFPPFPPNFPTERSVALDNDTDATLIGLQFNLPIFSGGGTR
                     SRVRQQEYQWQAARERLERTSRQTERQARDAFLGVTSEISRVNALKQALESSRTALQA
                     TEAGYEVGTRTTVDVLEARRNLVQAFTNLSRSKYDYLLNVIRLRQAAGTLDPAALEEI
                     NGWLDETVPTTPAPPTAEPAAASPAP"
     gene            complement(11951..13294)
                     /gene="waaA"
                     /locus_tag="PKN91_05625"
     CDS             complement(11951..13294)
                     /gene="waaA"
                     /locus_tag="PKN91_05625"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF004388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HNS27588.1"
                     /translation="MTPQGGRFAPMRLLYLTAVYLLAPLVGAAMLLRGFRDRSYWMNF
                     SERFGFGTVEQEGRCLWVHAVSVGEVQASAALVRALRERYPDLPLVVTTVTPTGAQRA
                     RALFEGIAHVRYVPYDLPGAVRRFFDRVRPRVAIILETELWPNLYHECGRRGVPLVLA
                     SARISPRSIGRYRHLAALFREALANGIVIAAQSPADAARFRHIGANPLRTHVMGNIKF
                     DFALPEAAAERGAAIRREHAAGRPIWVAGSTHAREEDILLAAHRRVLTSHPDALLLLV
                     PRHPSRFAEVRGWLERQGVRFASRSRAEQCQPDTSVYLGDTLGELVILYAAGDVAFVG
                     GSLVPIGGHNLLEPAALGLPLLTGPHTFNAEDIAQLLISAGAAEIVTDADALGARVAE
                     LFADEAARRRIGATGKSLVEANRGALSRLLALLEPVIAATEPATPLPAPRSAAPV"
     gene            complement(13291..14037)
                     /locus_tag="PKN91_05630"
     CDS             complement(13291..14037)
                     /locus_tag="PKN91_05630"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: protein motif:HMM:NF002475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HNS27589.1"
                     /translation="MGRTWASGSEVKREAIAGGAMLYDSSRAGNLRAAWFDPAYWRKR
                     AAVTGEAPGRGSTLFFAADGREYALRHYRRGGLAARLSKDRYLWRSEVTTRPFHEFHL
                     SYHLANAGLPVAPPVAARYVRHGALYSGDLITERLAGTLSLAAALDRGAVQLLVWVGI
                     GRCLRRFHDLGVCHADLNAHNIMLDMEGTVFLIDFDRATLRGPGLWRDANLARLRRSL
                     DKIADPMPEGRFTEADWHSLLAGYREGGGT"
     gene            14058..15110
                     /locus_tag="PKN91_05635"
     CDS             14058..15110
                     /locus_tag="PKN91_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNS27590.1"
                     /translation="MLPLAAPPRSVCILRLSAIGDTCHVVPVIRTLQRAYPQARITWI
                     IGRLEARLMTLLPDIEFITVDKRAGVKAARTLRAQLAGRRFDVLLHMQLALRASLFAR
                     CVRARVKLGFDRARARELQWLFTNARIAPRTREHVLDSFFGFTEALGIEDRVLDWSLP
                     LPADAQAYAARLVPDSTPTLLISPCSSHPLRNWSAARYAAIADHAAARHGMRIILCGG
                     PSAAERQMGEAIVAHAGTPLVNQIGQDTLPQLLALIGAATVLVSPDSGPAHMATMVGT
                     PVVGLYAATNPARSGPYLSRQWCVDAYAEAARRFRGKTPDELPWTTKIEEPGVMDLVT
                     VEAACAKLDALLASRR"
     gene            15107..15499
                     /locus_tag="PKN91_05640"
     CDS             15107..15499
                     /locus_tag="PKN91_05640"
                     /inference="COORDINATES: protein motif:HMM:NF039364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6165 family protein"
                     /protein_id="HNS27591.1"
                     /translation="MKDILVPVSPGELLDKITILRIKSARMTDPAKLANVRHELAQLE
                     ATWRASGAAVAEVAADEAALQRVNEALWDIEDEIRDKELAQAFDARFVELARAVYVTN
                     DERAAIKKRINTQLGSAIVEEKSYRPYR"
     gene            complement(15516..15713)
                     /locus_tag="PKN91_05645"
     CDS             complement(15516..15713)
                     /locus_tag="PKN91_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HNS27592.1"
                     /translation="MPATKTPLLQPNAQNQYEVTADDLPLSCPMPQMYLWNSHPRVYL
                     PIQQSGWAKCPYCGAEYTLRK"
     gene            complement(15743..18619)
                     /gene="glnE"
                     /locus_tag="PKN91_05650"
     CDS             complement(15743..18619)
                     /gene="glnE"
                     /locus_tag="PKN91_05650"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HNS27593.1"
                     /translation="MAAAAARAAGVLQELQQLPAVAQALAAAPPMVADSLPAVFAASD
                     FVAESCRRDPQLIPALIESGTLLAPRALADYAASAAAEGQWPDEPQFQSWLRRWRCRE
                     LVRIAWRDLAGWAGVEATIGELSAFAEAAVKVAYEFARAPLVARFGTPRGADGAPQPL
                     VVLSMGKLGGGELNFSSDIDLVFLFPEFGETDGARPIANEEFFTRLGQALIRLLDAPT
                     VDGFVFRVDMRLRPFGDSGPLVAGFSSFEDYLLRHGRAWERYAYVKARALTAADAYAE
                     VQANAVRPFVYRRYIDYGVFESLREMKGLIVREVQRRDLEDNIKLGPGGIREVEFIVQ
                     ALQLVRGGQDRRLQTPSLLEALCVIEGGRLLPRGAAAELREAYCFLRRLENRLQMLQD
                     RQTHELPTDAESCARIAFAMGSPDWESLVATLAAHRARVSAHFKAVVFAPEVDAATPV
                     ITVDLGPLWDDGSGADRTLMERTLATAGIAEPAEAARLLIEFRGTIERRLDAPGRKRV
                     RALMPAVVADIARAAGRLDVLRRVLRVFEAIGSRSAYFALLHESPAARRRLVDLCVHG
                     DFLAAQIAAHPLLLDELIDDRLIEALPDRAALSRDLEFRLEQASDEDPERQVELLRHF
                     QRAAIFRIAVADLTGRLPVMRVSDRLTDVAELIVEKAMQLGWRQMVAQFGAPMCGEGE
                     ARREVNICAVGYGKLGGMELGYTSDLDLVFLHDSAGEAQETHGAKPIDNQVFFVRLAQ
                     RIVHLLTMHSPAGRLYEVDMRLRPSGKGGMLVTSIAAFAEYQRTEAWTWEHQALLHAR
                     AVAGPAPLRAQFEAVRLDSLRDAVRRDSLRDDVRAMRERMRRELSRAPAGQVDLKQDP
                     GGIADIEFLAQYWALRWAGEHPPVAFYSDTIRQLESVASADLVPQATVDVLTRAYRLY
                     RERIHHRALADAPPIVPESDFAAERAAVIAIWDEAMGDQPISGL"
     gene            complement(18648..19514)
                     /locus_tag="PKN91_05655"
     CDS             complement(18648..19514)
                     /locus_tag="PKN91_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27594.1"
                     /translation="MQNVNLAHCAPWIIACTLCLGACTRETPEEAATAAAPGEPAAES
                     AAGADEAILAWAEQALARNPALEVVATDRKAGIFTVRTRMGGELRTIRMDELVAAPPG
                     VDDATIESDSAPPMADVRAAPGASSASEEASAPAITVTREAGRLSITGPGVSIASAGP
                     PAAAAAAAAAGEAPPGARVEQRRNQPIVCQGSRLMQIDGRTIDFDGDGILVEDGCDLH
                     LTNSHITAGGTAVTVIGGRVHIVNSTVKGARGSIEASRGSQVFMSGASIDGMQRRFDT
                     AQINDLGDNRYR"
BASE COUNT         3074 a         6877 c         6699 g         2952 t
ORIGIN      
        1 tcggcgacaa cgtcggcgac tgcgccggca tggcggccga cctgttcgag acctatgcgg
       61 tgaccctcgt cgcgacgatg ctgctcggcg gcctgatgct gcaggaccag ggcgcggccg
      121 cggtgaccta cccgctggcg ctcggcgcgg cctcgatcat cgcctcgatc atcggcacct
      181 tcttcgtcaa gacgagcgat ggcggcaaga tcatgaacgc cctctacaag ggcgtgatcg
      241 tctcgggcgt gatcgcggcc atcgcgttct ggttcatcac cgattcgctg atgccagagg
      301 acgccggcgc gatgttcggc tgcgcgttga tcggcctagt gctgaccgcc gcgatgatcg
      361 tcatcaccga gtactacacc gcgaccgagt actcgccggt gcgcaaggtg gcgggcgcct
      421 cacagaccgg ccacgcgacc aacatcatcg cgggcctcgg tgtgtcgatg aagtcgaccg
      481 cgctgccggt gctcgccgtg tgcttcgcga tctacggcgc gtacgaactc ggcggcctct
      541 acggcatcgc catcgcggcc acctcgatgc tgtcgctgac tggcatggtc gtggcactcg
      601 acgcctacgg cccgatcacc gacaacgcgg gcggcatcgc cgaaatggcc gaactgccga
      661 agaagatccg cgacatcacc gatccgctcg acgcggtcgg caatacgacc aaggcggtca
      721 ccaagggcta cgcgatcggc tcggcaggac tcgccgcgct ggtgctgttc gccgactaca
      781 cgcacaacct cgaaacccac ctgctggcga ccggccgcga gatgatcgag ttctcgctgt
      841 ccgatccggc ggtgatcatc ggcctcttca tcggcggtct cgtgccctac ctgttcggcg
      901 ccatggcgat ggaggccgtg ggtcgcgccg ccggctcggt ggtgaatgaa gtgcgccgcc
      961 agttccgcga gatcgcgggc atcatggaag gcagggcgaa gcccgactac tcgaaggccg
     1021 tcgacctgct gacccgtgcg gcgatcaagg aaatgatcgt tccctcgctg ctgccgatcc
     1081 tggtgccggt catcgtcgca ttcggcctga acgcgctgat gggacccggc gccggcctgc
     1141 gcgcgctcgg cggcctgctg atcggcacga tcgtcaccgg tctgttcgtc gcgatctcga
     1201 tgtgcaccgg cggcggcgct tgggacaatg ccaagaagta catcgaggaa gggaacttcg
     1261 gcggcaaggg ctccgaagcc cacaaggcct cggtcaccgg cgataccgtg ggcgatccgt
     1321 acaaggacac cgcgggcccg gcgatcaatc cgctgatcaa gatcatcaac atcgtcgcac
     1381 tgctgctcgt accgctgctc tgacacctgc cagggagaag cccatgacca ccaagcgcat
     1441 gattctcgcc gccgcactcg gcagcctcgt ctcgctcggc accgccgtgg cggccgacga
     1501 gaaggccgaa accgagaagt gctacggcgt cgccgaagcc ggcaagaatg attgccacac
     1561 ggcgagccac gcctgcgccg gcctcgccac gaccgacaag gacccggccg aatgggtcag
     1621 ggtgccgaaa ggcacctgcg agaagatggg cggcaagctg gaagcgccgc ggcccatctg
     1681 accgcgctgc cgtaggtcgc gaaggtcgcg gtcgcaccgc cggactgcac tgcgccgcgc
     1741 caggccatgc cgccgtcgac acccacccgc tgcggcatcg gcctgcgcgc cgcccaccac
     1801 cgcgaggtgc tgcgcaccgc gccggacgtc ggctggttcg aggcgcacag cgagaactac
     1861 ttcgccgatg gcggcggcgc gctcgacacg ctgctacgcg tgcgcgagcg ctggccggtc
     1921 gcgctgcacg gcgtcggcct cgggcttggc tcgaccgccc cgctcgatcg cgagcatctg
     1981 cgccgcctcg cgcgcctcgc cgcgcgcatc gagccctggg ccgtatcgga gcacgtgtgc
     2041 tggggccagg cggacgggcg gcacaccaac gacctgctgc cgatgccgcg caccggggaa
     2101 tcgctgcgcc acctcgccgc gcgggtcgcc gagctgcagg acgcgctcgg ccgccaggtc
     2161 ctgctcgaga acgtctcgag ttacctggag ttcaccggcg cggaattcac cgaagccggt
     2221 tacctcgcgg cgctcgtggc cgaatcgggc tgcgccctgc tgctcgacgt caacaacgtg
     2281 tacgtgaacg cgtgcaacca cggtttcgat gccgaggcct atttcgacga actgcccgtg
     2341 gatgccgtgc gcgagatcca tctcgcgggc cacgagccga tccggatcgc cggccgagta
     2401 atccgcatcg acacccacga tcgaccggtg tgcgatgagg tctgggcgct gtgcgcgagc
     2461 gcccggcgcc gcttcggccg cgtgccgacg ctgatcgaat gggacgcgga cctgccgccg
     2521 ctcggagtac tggtcgcgga ggcgcaacgt gccgatgccc ttggatgagt tgcagcggcg
     2581 cttcgtcgcg agcctgtacg gcgaggctgc ggacctcgac ggggcgatca gcggcgctgg
     2641 cgggatcagt gccacggagt gcctcgcgat ctaccgcggc aacctgcatg tggccttcgc
     2701 caaggcgctc gagctcgggt ttccggtgct cgccgcactc tgcggaccgg cgttcttcac
     2761 cgtgctcgcg cgcgagttcc agcgggcgca cccctcggct tcaggtgacc tgcaccacat
     2821 cggcgggccg cttccgcact acctgcgcca gcgctacgcg gggagcgagt tcgcctactt
     2881 cgcggatgtc gccgccctcg agtgggcgcg cgaagaatcg gcacgcgccg cggacgcggc
     2941 gccgctcgac ctgcgcgggc tgagcgcggt tgccgacgac gcggcggcct cggtgcgctt
     3001 cgcgctgcat ccgggtgtgc ggctcgtcgc gtcgcgctgg cccgtcctcg cgatctggga
     3061 agcacaccag gaccctggcg gagtgcgcgg ggtcgatctc ggagcgggcc cggaacaggt
     3121 gctcctgcgg cgcgcagcga gcgggctcgt gctcgaacgg atctccgccg ccgaattcga
     3181 cctgctgacg accctcgggc ggggcgagcc gctcggcgca gcgttcgacg cggcaatcgc
     3241 ggcggattct acgttcgacg tcggcgcagc gctgcgacgc ttcgtcgcgg gcaacgtgtt
     3301 cgcgagcatg ggcgactagc gcccgagcag tccctcgccg atccagccct tcagcatcgt
     3361 tgccgcgcac cgggctgcat cgcgctcgcc cgtctcttgc gcgagccccg cgcagagcgc
     3421 cgcaaagctt gcgcccccgg caagccgtgc gagcgcccgc ccctcggccg gcgtcgtcga
     3481 acggaacctg atcgcaggat cccgccgcca caccacccac gacaccgggg ccgtcgcgcg
     3541 cggttcgggg caaggcctgt cctcgctcac ggcctgccag cactcgacgg cattggtggt
     3601 ggcctcgaag cgccgcaccg tgcggtgcca ccgcaagcgc aacgcgggcc aatccgccgg
     3661 cgcaacgcgg gcgatatccg cttccgtcat cgcgaagtcg tcgccggcat cgaacacctc
     3721 ggcgagcagc cattcccagc gcgcgaggtc ggcgtgcatg ccgcggagat gacgatgaag
     3781 atctgctccg taccagcgga tcgacgcgtg ggtcggcacc gcgcccgcaa tgtaggtcgc
     3841 ggcagcggcc tcgaaggctg ccacaccgag cagcttcctg agcaccggat aggtcacgcc
     3901 gagcgcctcg gtgagccgtg cccgatatgc gtattcgtag atgccgagcc gtgtggcgac
     3961 ttcccgtgcg tcaccggcga cgctgccttc gatcgacgga tcgccgcaca gcacccgctg
     4021 ctgcaacgcc gtctgcaagg caacgagcgt gctcacgccg cggctccgag tgcactcgcc
     4081 gcgatggcgc gcgcccgatc gagctcggcg acgagcgcct cgagcggcgg gatccggtca
     4141 tcacgctcga tcatcgtcgc ggcatgcggg aagcgcgccg ccgcaatttc gtagagtcgc
     4201 cacacggcct cgtccaccgg cgcatcgtgc gtatcgacga ggaagcggcc ctggcggctg
     4261 tggcccgcga ggtgcaactg tcgcacccgt tcgacgggaa tcgcgcgcag gtacctggcc
     4321 gcgtcgaaac cgtggttcac gctgttgacg tacacgttgt tgacgtcgag cagcagcagg
     4381 caatcggcgg cctcgcagac gctcgtgacg aactcccatt cggtcatctc tgcgcccgtg
     4441 aagttcacgt agctcgagat gttctcgagc aggatctgcc ggccgagaca gtcctggacc
     4501 tggcgaacgc gcgccgcaac gtgcgcaacc gcttcggcgg tatgcggcag cggcagcagg
     4561 tcgtgcaggt tgacgccgtc gatgccggtc cagcagaggt ggtcggaaat gcctgccggc
     4621 tcgatccgcc gcgcaagccg cggaccgagc gcaggtaggc cggatcgagc ggtgccgtgg
     4681 agccgaccga gagcgacacg ccgtgcatca cgagcgggta gcgcgcgcgc acccgttcga
     4741 ggtagcgcag cggcttgccg cccggcacga ggtagttctc ggtcgtcacc tcgaaccagt
     4801 cgatgcggtg cgtgccaccc agcaccgcgt cgtagtggtc ggggcgcagg ccgaggccga
     4861 acccgagctg tcgaatggtc atctccgcgg caccgccgcc attaccggcc cggtgcccgc
     4921 ctcacatcgg gtccttcgcg ccggcctcgg ccttcgccgc atcgcattgg ggctgcgtga
     4981 gttcgaggaa gcccttgccc ttgcacgagt tctggccctt gcacgcattg ttcgcggtct
     5041 tgcacgccga ctggcccttg cacgagttga cgcccatgca gtgcaccttg gcttcgtccg
     5101 cggccgacgc gggcagcgcg agcgcgccga acagcaatgc ggcggccgtc gcgatcgcgg
     5161 cgccgttcga ctttacggaa tcgatcatgt gtgagctcct tctctgttgt catgggatgg
     5221 cgactacgcg aaggagaccg cgcgagacgc cggaaaattt catcgcgggt cctgacggcg
     5281 gatccactgg catgtaatga tccggccgat ggcccggtcc aactttcgag agcggaagtt
     5341 cgaggagctg tgcgcaccgc tgaaggcgga tctgctgcgt tacgccttct ggctgtgccg
     5401 cgaccggtcg gtcgccgagg acatcgtcca ggaggcgttg ttgcgggcgt ggaagtcgat
     5461 cgacgggctc gagagtgccg cagcggtgaa gccatggctg ctcacgatcg tgcgtcgcga
     5521 gttcgcccgc accttcgagc gcaagcgcca cgagaccgtc gacatcgcgg cgctcgacgc
     5581 gtgccaggac atcgcgctcg cttcacccga ggaggatccc gagatcaacg ccatgcgcca
     5641 ggcaatgctc aggctcgagc cgatctaccg cgaacccctc gtgttgcagg tgctgttcgg
     5701 ctacacgacc gccgagatcg ccgcgcacct cgcgatcagc gtgcctgccg tgctcacgcg
     5761 tctctaccgg gcgcgcgaga agctgcgcgg ggtactcgcg tgaactgcga ggaattccgc
     5821 gcggcgaccg gtgcggaccc gcacgcaacg cgcgcggacc ttgcggccca cgcggcgggc
     5881 tgcgaggcgt gcgcggcctg gcgacgtgag ctgctgcgga tggacgacgt cattcgccgc
     5941 gcgctcgcgt tcgatgtcgg ggcggagcgg accggcggcc cggcgttgcc cgcacgatcg
     6001 gcgtggcggc ccctgtcctg gcgcggctgg gcgatggccg cgggtgtcgc ttgcgccacg
     6061 cttgtcgccg gcctcctctg gctcgcgcct gcgcgcaccg cgctcgcgga cgcggtggtc
     6121 gcgcacatga gcgaggagcc gggcgcgtgg gcccgcaccg acagccccgc cgatcccgtg
     6181 ctcctggcgc gtgtgctggc gcgtgcgggc gtgcgcctga acgccggcgc aggcgtcgcc
     6241 tcgtacgcca acagttgctg gtttcgcgga catcacgtgc cgcatctcgt tttccagacc
     6301 gaggacgggc ccgtgaccgt catggtgctt cccgccgagc gtgtcgcggc ccccatcgag
     6361 ttcgacgagc agggatatcg cggcgtgctc gtgccagccg aacacggcgc cctcgccatc
     6421 ctgtcgcgcg gcgcgatgcc gagccgcgaa ctcacggcac ggctgcaggc cgcgttcacg
     6481 tactccgcaa aataagcttg cggttcgtgc aataacggca cgacctgcgc ggtctccttc
     6541 caggtccgac cccagaccag gagtgcaacg gaatgaccag gaacatcgtg ctcgcgagtg
     6601 ccctcggcag cctgctcgcg ctgaacctcg cgacggcgag cgccgcgcaa gcagctacgg
     6661 aaaagtgcta tggcatcgcc atggcgggca agaacgactg cgcaacggcc gcgcacgcct
     6721 gcgcgggtca gtcgaaaacc gaccgggacc cccgcgaatg gcagctggtg ccaaagggca
     6781 cgtgcgagaa aatgggcggg cgcctggcgc cgacatcgcc gcaatgagcg tatcggctgc
     6841 atatgcggcg gcgaaccgcg cgctcgcatc ggtcctcgat cgcctgcgca cgccactgct
     6901 gctcgcctgc cgcctctacg tgtcgtggca gttcctgaaa tccggctggc tcaagctcgg
     6961 cgactgggac acgacgctgt ggctcttccg cgaggagtac cacgtgccgc tgctgccgcc
     7021 cgagtgggct gcagtggcag gcacggtcgg cgaaacctta ttcgcgctgt ccctgatcgc
     7081 gggcttcgca agccgcccgg cggcactcgg gctctttgcc gtcaatgtgc ttgcggtgat
     7141 ctcctaccgg cacgtcctgc ttgcgcccgg ctacgaagcg gcgctcgcgc agcatgtgct
     7201 gtggggtttc atgctgctcg tgctgatcgc cttcggcccg ggccgcgtat cggtcgatca
     7261 ggccgccatc ggcaacggcg tcgggcgcgc gatcgcgtag ccctgcaggt agtcgaaccc
     7321 catggtgcgc gccgcctcga gcgccgcgtt gtcctcgacg gcctccgcga tgatgcggaa
     7381 atcgagggtg cgcgcgagct tgatcatcgc gccgaccatc gcctggttga cgaggtcggt
     7441 accgaagttc ttcatgaacg agccgtcgat cttcacgaaa tctaccggca gcgtcttcag
     7501 gttcacgaac gagcccgccc ccgcgccgaa gtcatcgaga gcgaactggc agcccatgct
     7561 gtgcagcact tcgatgaatc gccgcgcctg ctcgagattc tccgacacgg cggcctcgct
     7621 gatctcgaag cacacgcgcg acggtgcgac cccggtgcga tcgaagcagt cgaccacgaa
     7681 ctcgaggaac tgcgagtcat tgagtgtctg gccggaaata ttgatcgcga ccgagcggcc
     7741 gaccggcgtc tggatcgcac cgcggccgag ggccgccagc gtggtctgca ccacccagcg
     7801 atcgatcagt gtcgtgagcc ggtagcgctc cgcggcgtgc atgaacgcgc cgggagcgac
     7861 atcgttgccg gccgtgtcgc gcagtcgcac cagcacctcg agcgcgggac cgctgccgtt
     7921 cgtgccgagc gccggcacga tcggctggtg gtagagctgg aagcggttct ccttgagggc
     7981 gccctgcagc cgatgcagcc actgcagctc gccgctctgg cgcgcgagga cctcatcgcg
     8041 cgcggagtac acggcgacct ggcccgaacc ctggcgcttg gcgacatagc aggccgaatc
     8101 ggccgcgatg agcacttctt ccatcgtgcc cgactcgtgc gacagctcca cgatgccgat
     8161 cgagatgccg atgttgaaga gcctgtcgcg ccagacgaag cggtgctcgg cgaccgcgcg
     8221 acagacgtcg tcggcgatct gccgcgcctt gtcgagcggg cagccgagca gcagcatgcc
     8281 gaactcgtcg ccgccgatgc gcgccacggt gtcggagtcg cgcaccgaat cgcgcaggat
     8341 cttcgccact tcgcgcagta ggctgtcacc ggccacgtgg ccgctcgtgt cgttgacgag
     8401 cttgaaacgg tcgaggtcga ggtagcagag gatgtgcgtg ccgtcgccga gccgggccgc
     8461 ctcgatcgcc tcaccgagcc gacgttcgaa ctcgcgccgg ttgacgaggc cggtgagcgc
     8521 atcgtgcgtg gcctggtagg acatctggcg cgccataccg cgcagttcgg tgacatcgtg
     8581 cagcagcacg acggcacccg tgaccttgcc cgcggcgtcg cgcagcggcg aggtgagcgg
     8641 ctcgatcgca cgctcctcgc cggtctcgtg cgagaccagc agtgcccggc gcggcgcatg
     8701 cacggacgaa ccgctgtcga tcgcatggcg gatcggatcg gcgatcgccc gccgttcgac
     8761 ttcgtcgacg agcccgacca catcctcgaa gtgccggccc tgcgcctcga ggcgcgacac
     8821 gcccgcgagc gcctcggccg cggcattcat gtattcgatg ctgccgtgcg cgtcggtgac
     8881 gacgatgccc tcggcgagcg actcgagggc gtcctcgcgt tcgctgcgcg gtgccggccc
     8941 gccgccctcc cccgccgcga tggcgaggtc cggcgcccgc aacgagggca cggtctgggt
     9001 cggcaccact tcgaccccgg tcacgagcac cgcggggccg ccgtcgtaat cgatgatcgt
     9061 cgtcgaaatc tccagccggc tggcgacacc ctgcagcccg atgatgtcga tctcgtagcg
     9121 gtcggccgtc gcctcgccgg cgagcttgcg gcgcaggttc tgttcgacga gctcggcgct
     9181 gtcggccgcg acgatgtcgg ccagcctgcg gccgatcagc accgcgcgat cggcgccgac
     9241 gaggctggcg aactggcagt tggcgtacag gatgacgtcg cgatggacca gcacgacttc
     9301 atggatgcga tcgccgagat ccgcgaacag cgcggggctc agccgctcgt ggtcgccctg
     9361 cgcggcgacc cggcccagca ggtgattgat cgccgtgccg agggcggcga gttcgggctg
     9421 gtcggtatcg aggtcgacgc gcccggtgag gcgcccgccg atcgcgatgc gctggacctg
     9481 ctgcgacagg gccgagacgg ccttgagttc acgccgctgc aacaggtaca gcacgagcag
     9541 gaggattgct accagcccga gaagtacgtt aactagcccg acgatcgaca tcggttatag
     9601 gcaatcccgt agattattgt tgtctgcgct cgcagcgctc gaatctgctg gggccagcat
     9661 ataggagccc ggccggggcg cggtgctgat ttcacgataa gcgtgcggcg cagcttggca
     9721 cggccgcgga aaacccttaa cttggcgcgc cctttcgtgc ggcagaataa gtccaatccg
     9781 gagcaagcgt ggtagcgcca atgtcgacag agttcgccct cgaccagatg gtcacgcaac
     9841 aggtacgcgc gtgggacgtg ctcgacgagc gcgtgctcga cgtcatgcgc cgcgtgcctc
     9901 gtgagcgcta cgtgccgcac gcctaccgcg aagtcgccta tgccgacacc gcgatcccgc
     9961 tcgcccacgg gcagcgcatg cttgcgccga aagtcatcgg ccgcatcctg caggcggtcg
    10021 aagcctccgc gcgcgacgcc gtactcgaga tcggcacggg ctcgggctac ctcactgcct
    10081 gcctggcggc cagcgcgcgc gtcgtgcact cgatcgacat ctacgacgat atcgccgccg
    10141 cggcgcgcga gcggctcgcg gcgaacggcg tcgccaatgc cgaggtacgc acggccgacg
    10201 ccttcgccga gcagatcggg acgcgctaca acgtgatcgt gctgaccggc tcgctgccct
    10261 gttacgacga gcgcttccag gaagcgctcg aagctggcgg gcggctgttc gcgatcgtcg
    10321 gcgaggacca ggcgatgtcc gcgcgcctgg tgcggcgcgt ctcggccagc agctggacca
    10381 gcgaggacct gttcgagacc tgggtcgaac cgctggtgca cgcaccgcgg ccaccccgct
    10441 tcgtcttctg atgcaccccc cttgaccaag gggtgtgata tagtgatata gtacactatc
    10501 tgttgatcac tatctccttg aattaaggga atttttatgt cgcgcttgcc gattttggga
    10561 tccgtggtgc tgatgttttc ccaggccgtg ctcgccgagg atctgctcac cgtgtaccag
    10621 cgggcgctcg ccgccgatcc cttgatccgc gaggccgatg ccacccgcct tgccgtgcgg
    10681 gagtcgaagc cgcaggcgct cgccgccctg ctgccgcaga tcagcggcac cgccggctac
    10741 gacaagaaca agttcgaggg cacgagcgac caggcccagg tcgtgagcga aggcgcggcc
    10801 gccggctcca ttacgatcct cagcacctcc ggcacacgcg agccggaaac ggaaacctgg
    10861 tcgctcgacc tgcgccagag cgtgttccgc tgggagaact gggtcgcgct gcgccgcgcc
    10921 gacaagcagg tggcccaggc cgaagccgat ttcgcggccg ccgagcagga cctgatcctg
    10981 cgcgtgtcga ccgcctactt cgacgtgctc gccgcgcgcg acacgctcga agcccagcag
    11041 gccaacctgc aggcggtctc gcgccagctc gaccaggccg agaagcgctt cgaggtgggc
    11101 ctgatcgcga tcacggacgt gcaggaatcg cgcgccgcgc gcgacaacgc agcggcggcg
    11161 gtgatcgcgg cgaagcgcca gctcgcgacg tcggaggaac tgctgcgcga agtcactggc
    11221 gagaagtact cgaccctcga caagcccggc gacgacctgc cgctgaagtc cccgcagccg
    11281 aacctcgagg acgactgggt gcgcgtcgcg atggaccaga acctcgcgct ggtctcgagc
    11341 cgcctggcgg ccgatatcgc ccgcgacgac gtgcgcacgg cgctcggcgg ccacctgccg
    11401 acgatcgatc tcgtggcgag ctatggcaac cagaagacca cgaccgaaca gaccttcccg
    11461 ccgtttccgc cgaacttccc gaccgagcgt tcggttgcgc tcgacaatga caccgatgcg
    11521 acgctgatcg gcctgcagtt caacctgccg atattcagcg gcggcggcac ccgctcgcgc
    11581 gtgcgccagc aggaatacca gtggcaggcc gcacgcgaac gtctcgagcg cacgtcgcgc
    11641 cagaccgagc ggcaggcgcg cgacgctttc ctcggcgtca ccagcgagat ctcgcgcgtc
    11701 aacgcactga agcaggcgct cgagtcgagc cgcacggcgc tgcaggccac cgaggcgggc
    11761 tacgaagtgg gcacgcgtac caccgtggac gtgctggaag cgcgccgcaa cctggtgcag
    11821 gcgttcacca acctctcgcg cagcaagtac gactacctgc tcaacgtgat ccgcctgcga
    11881 caggccgcgg gcacgctcga tccggccgcg ctcgaggaga tcaacggctg gctggacgag
    11941 accgtgccga ctacaccggc gccgccgacc gcggagccgg cagcggcgtc gccggctccg
    12001 tagcggcgat caccggctcg agcagcgcca gcaggcgcga cagcgcgccg cgattcgcct
    12061 cgaccagcga cttgccggtc gcgccgatgc gcctgcgcgc ggcttcgtcg gcaaacagct
    12121 ccgcaacgcg cgcaccgagc gcatcggcgt cggtgacgat ctcggcggcg ccggcgctga
    12181 tcagcagctg ggcgatgtcc tcggcattga acgtgtgcgg cccggtgagc agcggcaatc
    12241 cgagcgccgc cggctcaagc aggttgtggc cgccgatcgg cacgaggctg ccgccgacga
    12301 aggcgacgtc ccccgcggca tagaggatca ccagctcgcc gagcgtgtcg ccgaggtaga
    12361 cgctcgtgtc gggctggcac tgttccgcgc gtgagcgcga ggcgaagcgc acgccctggc
    12421 gctcgagcca gccacgtacc tctgcgaaac gcgacggatg gcgcggcacc agcagcagca
    12481 gcgcgtccgg atggctcgtc agcacgcgcc ggtgcgcggc gagcaggatg tcctcttccc
    12541 gggcgtgcgt gctgccggcc acccagatcg ggcgtcccgc ggcgtgctcg cggcgtatcg
    12601 ccgcgccgcg ttccgcggcg gcctccggca gcgcgaagtc gaacttgatg ttgcccatga
    12661 cgtgggtgcg cagcggattg gcgccgatgt ggcggaatcg cgcggcatcc gccggactct
    12721 gtgcggcgat cacgatgccg ttggcgagcg cttcgcgaaa cagggcggcg aggtgccggt
    12781 agcggccgat ggaccgcggc gaaatgcgcg cgctcgcgag cacgagcggt acgccgcgcc
    12841 ggccgcattc gtggtagaga ttgggccaca gctcggtctc gaggatgatc gcgacgcgcg
    12901 gccgcacgcg atcgaagaag cgccgcaccg cgcccggcag gtcgtagggc acgtagcgca
    12961 cgtgggcgat gccctcgaac agcgcgcggg cgcgctgcgc gccggtgggc gtcaccgtgg
    13021 tcacgacgag cggcaggtcc ggatagcgct cgcgaagcgc gcgcaccagc gccgccgacg
    13081 cctgcacttc gccgaccgac accgcgtgca cccacaggca ccgcccctcc tgttccacgg
    13141 taccgaagcc gaagcgctcg ctgaagttca tccagtagct gcgatcgcgg aagccgcgca
    13201 gcagcatcgc ggcgccgacg agcggcgcca gcaggtacac ggctgtcagg tacagcagcc
    13261 gcatcggcgc gaagcgaccg ccctgcgggg tcaagtgccg ccaccctcgc gatagccggc
    13321 gagcagcgaa tgccagtcgg cttcggtgaa ccggccctcg ggcatcgggt cggcgatctt
    13381 gtcgagcgag cggcgcagcc gcgcaaggtt cgcatcccgc cacaatcccg gtccgcgcag
    13441 tgtcgcgcgg tcgaagtcga tcaggaatac ggtgccttcc atgtcgagca tgatgttgtg
    13501 cgcattgaga tccgcatggc acacgccgag atcgtggaac cggcgcaggc agcggccgat
    13561 accgacccag acgagcagtt gcacggcacc ccggtcgagc gccgcggcga gcgagagcgt
    13621 gccggccagg cgctcggtga tcaggtcgcc gctatagagt gcgccgtggc gcacgtagcg
    13681 cgcggcaacg ggcggcgcga ccggcaggcc cgcattcgcg aggtgatagc tgagatgaaa
    13741 ctcgtggaac gggcgcgtcg tcacctcgct gcgccacagg tagcgatcct tcgacagccg
    13801 tgccgcgagc ccgccgcgcc ggtagtggcg caatgcgtat tcgcggccgt cggcggcgaa
    13861 gaacagcgtc gagcctcgcc ccggcgcctc accggtgacc gccgcacgct tgcgccagta
    13921 cgccgggtcg aaccacgcgg cgcgcaaatt gccggcacgg gacgagtcat atagcatcgc
    13981 tccgcccgcg atggcctccc gcttcacttc cgatccgctc gcccaggtac gccccatact
    14041 cgctcatccc atcgcccatg ctgccactcg ccgcgccgcc ccgtagtgtc tgcatcctgc
    14101 ggctctcggc gatcggcgac acctgtcatg tcgtgccggt gatccgcact ctgcagcgcg
    14161 cgtatccgca ggcccggatc acgtggatca tcggtcgcct cgaggcgcgg ctgatgaccc
    14221 tgctgcccga catcgagttt atcaccgtgg acaagcgcgc cggcgtcaaa gccgcgcgca
    14281 cactgcgcgc gcagctcgca ggaagacgtt tcgacgtgct gctgcacatg cagctcgcac
    14341 tgcgcgcgag cctgttcgcc cgctgcgtgc gcgcgcgcgt gaagctgggc ttcgatcgcg
    14401 cgcgggcgcg cgagctgcag tggttgttca ccaatgcgcg cattgcgcct cggacccgcg
    14461 agcatgtgct cgacagtttc ttcggtttca ccgaggcgct cggtattgag gaccgcgtgc
    14521 tcgactggag cctgcccctg cccgcggacg cgcaggccta tgccgcgcgg ctcgtgccgg
    14581 attcaacccc gactctcctc atcagcccgt gctcgagcca tccgctgcgc aactggtcgg
    14641 ctgcgcgcta tgccgcgatc gccgatcatg ccgccgcgcg ccacggcatg cgcatcatcc
    14701 tgtgcggcgg tccctccgcg gccgaacggc agatgggcga ggcgatcgtt gcgcacgccg
    14761 ggacaccgct cgtgaaccag atcggccagg acacgctgcc gcagcttctt gcgttgatcg
    14821 gcgccgccac cgtgctcgtg tcgcccgact cgggtccggc ccacatggcg accatggtcg
    14881 gcacgccggt cgtcggcctt tatgcggcta ctaacccggc acgcagcggt ccctacctgt
    14941 cgcggcaatg gtgcgtcgat gcctatgccg aggccgcacg gcgctttcgc ggcaagaccc
    15001 ccgatgaact gccctggacc acgaaaatcg aggagcccgg cgtcatggat ctggttactg
    15061 tcgaggcggc atgcgcgaaa ctcgacgcgc tgctagcatc gcggcgatga aggacatact
    15121 cgtaccggtc tcgccgggtg aactgctaga caagatcacg attctcagaa tcaagtcggc
    15181 gcgcatgacc gacccggcca agctcgcgaa cgtgcgccac gagctcgcgc agctcgaggc
    15241 tacctggcgc gcgagcggcg cggccgtggc cgaggtcgcc gccgacgagg cggcactgca
    15301 acgggtgaac gaggccttgt gggacatcga ggacgagatc cgcgacaagg agctcgccca
    15361 ggccttcgac gcgcgcttcg tcgaactggc ccgcgcggtg tatgtcacga acgacgagcg
    15421 cgcggcgatc aagaagcgca tcaacacgca gctcgggtcc gcgatcgtcg aggaaaaatc
    15481 ctaccgaccc taccggtagg acccggcacc aggccctact tgcgcagcgt gtactccgcg
    15541 ccgcagtagg ggcacttcgc ccagccgctc tgctggatcg gcaggtagac ccgagggtgt
    15601 gaattccaca ggtacatctg cggcatcggg cacgacagcg gcaggtcgtc ggccgtgacc
    15661 tcgtactggt tctgggcgtt cggttgcagc aacggggtct tggtggccgg catgaagggt
    15721 gtcccctggg tcgttggcgg cattataagc cgctgatcgg ctgatccccc atcgcctcgt
    15781 cccagatggc aatgaccgcg gcgcgctcgg cggcgaagtc gctctccggc acgatcggcg
    15841 gcgcatcggc cagggcgcgg tggtggatgc gctcgcggta cagccggtag gcgcgcgtca
    15901 ggacatccac tgtagcctgc ggaacaagat cggcagacgc gaccgattcc agctggcgga
    15961 tcgtgtcgga gtagaacgcc accggcggat gctcgccggc ccagcgcagc gcccagtact
    16021 gcgccaggaa ttcgatgtcg gcgatgccgc cggggtcctg cttcaggtcg acctgcccgg
    16081 ccggcgcgcg cgacagttcg cgccgcatcc gctcgcgcat ggcgcgcacg tcatcgcgca
    16141 gcgagtcgcg ccgcacggcg tcgcgcagcg aatcgaggcg caccgcctcg aactgcgccc
    16201 gcagcggcgc aggcccggcc acggcgcgcg catgcagcag cgcctggtgt tcccaggtcc
    16261 aggcctccgt tcgctggtac tcggcaaagg ccgcgatgct ggtcaccagc atgccgccct
    16321 tgccgctcgg gcgcaggcgc atatccacct cgtacaggcg tccggcgggc gagtgcatgg
    16381 tgagcaggtg cacgatgcgc tgcgcgagcc gcacgaagaa tacctggttg tcgatcggct
    16441 tcgcgccgtg cgtctcctgc gcctcgccgg ccgaatcgtg caggaacacg agatcgaggt
    16501 cggaggtgta tccgagctcc atgccaccga gcttgccgta gccgacggcg cagatgttca
    16561 cctcgcggcg cgcttcgccc tcaccgcaca tcggtgcgcc gaactgcgcc accatctgtc
    16621 gccagccgag ctgcatcgcc ttctcgacga tcagctctgc aacgtcggtc agccgatcac
    16681 tgacgcgcat gaccggcaat ctgccggtga ggtcagcgac ggcgatgcga aagatcgccg
    16741 cccgctggaa atgccgcagc aactcgacct ggcgctccgg gtcctcgtcg ctcgcctgtt
    16801 cgagccggaa ctcgagatca cgcgacagcg cggcccgatc cggcagcgcc tcgatcagcc
    16861 gatcgtcgat cagctcatcg agcagcagcg ggtgtgcggc gatctgcgca gcgaggaaat
    16921 cgccgtgcac gcacaggtcg acgagccgcc ggcgcgcagc ggggctctcg tgcagcaggg
    16981 cgaagtatgc cgaacgcgag ccgatggcct cgaacacgcg cagcacgcgg cgcaggacat
    17041 cgagccggcc cgccgcgcgg gcaatgtccg cgaccacggc cggcatcagc gcccgcaccc
    17101 gcttgcggcc cggagcatcg agcctgcgct cgatcgtgcc gcggaactcg atcagcaggc
    17161 gcgcggcctc ggccggttcc gcgattcccg cggtcgcgag cgtgcgctcc atcagggtcc
    17221 ggtcggcgcc gctgccgtcg tcccagagcg gcccgagatc gaccgtgatc accggtgtcg
    17281 cggcgtcgac ttccggtgcg aacaccaccg ccttgaaatg cgccgacacg cgcgcacggt
    17341 gcgcagcaag cgtggcgacg agcgactccc agtccggcga tcccattgcg aatgcgatgc
    17401 gcgcgcagct ttcggcgtcg gtcggcagct cgtgcgtctg gcgatcctgc agcatctgca
    17461 ggcggttctc gagccggcgc aggaagcagt aggcctcgcg cagctcggcg gccgcgcccc
    17521 gcggcagcag gcgcccgccc tcgatgacgc acagggcctc gagcagcgag ggcgtctgca
    17581 ggcgtcggtc ctgcccaccg cgcacgagct gcagcgcctg cacgatgaac tcgacttcgc
    17641 ggatgccgcc gggcccgagc ttgatgttgt cctcgaggtc gcggcgctgc acctcgcgca
    17701 cgatcaggcc cttcatttcg cgcagcgact cgaacacgcc atagtcgata tagcggcggt
    17761 agacgaacgg gcgcacggca ttggcctgca cttcggcgta cgcgtcggcc gccgtcaacg
    17821 cgcgtgcctt gacgtaggca tagcgctccc acgcgcggcc atggcgcagc aggtagtcct
    17881 cgaacgagga aaagccggcc accagtggcc cgctgtcgcc gaacggccgc agacgcatgt
    17941 cgacgcggaa cacgaatccg tcgacggtcg gggcatcgag cagccggatc agagcctggc
    18001 cgaggcgagt gaagaactcc tcgttggcga tagggcgcgc cccgtcggtc tcgccgaact
    18061 cggggaagag gaacacgaga tcgatgtcgg acgagaagtt cagctcgccg ccgccgagct
    18121 tgcccatgct gagcacgacg agcggctgcg gcgcgccgtc cgcgccgcgc ggcgtgccga
    18181 agcgcgcaac cagcggcgcg cgggcaaatt cgtacgcgac tttgacggcc gcttcggcga
    18241 aggcggacag ctcaccgatc gtcgcttcga cgccggccca gcccgcgagg tcgcgccacg
    18301 cgatgcgcac cagctcgcgg cagcgccagc ggcgcagcca cgactggaac tgcggctcat
    18361 cgggccactg gccctccgcc gcggcagacg ccgcgtaatc ggccaaggcg cggggcgcaa
    18421 gcagcgtgcc gctctcgatg agtgccggga tcagctgcgg atcgcgccgg caggattcgg
    18481 cgacgaagtc gctcgcggcg aacaccgcgg gcagggaatc ggccaccatg ggcggcgcgg
    18541 cggcgagcgc ctgcgcgacg gccgggagct gctgcaactc ctgcaggact ccggcggcgc
    18601 gcgccgcagc cgcggccacg ccgatccgag gcagcgaagc gccgggctca gcgatagcga
    18661 ttgtccccaa ggtcgttgat ctgcgccgta tcgaagcgac gctgcatgcc atcgatcgac
    18721 gcgcccgaca tgaagacctg cgatccccgt gaggcctcga tcgaaccgcg cgcgcccttg
    18781 acggtgctgt tcacgatatg gaccctgcca ccgattacgg tgaccgccgt cccgcccgca
    18841 gtgatgtggc tgttggtgag atgcaggtcg cagccatcct cgacgaggat cccgtcgccg
    18901 tcgaagtcga tggtccgccc gtcgatctgc atgagtcgcg agccctggca gacgatcggc
    18961 tggttgcggc gctgctcgac gcgcgcgccg ggcggcgctt cacccgcggc ggccgctgcg
    19021 gccgctgcag ccggtgggcc ggccgacgca atcgacacgc ctggcccggt gatgctgagc
    19081 cggcccgcct cgcgcgtcac ggtaatcgcc ggggcgctcg cctcctcgct ggcggacgac
    19141 gcaccgggcg cggcgcgcac atcggccatg ggcggggcgg aatcggattc gatcgtggcg
    19201 tcatcgacgc cgggcggcgc cgcgaccagt tcgtccatcc tgatcgtgcg cagctcgccg
    19261 cccatgcgcg tgcgcacggt gaagattccg gccttgcggt cagtcgccac gacctcgagc
    19321 gcaggattgc gtgccagcgc ctgttcggcc cacgcgagta tcgcctcgtc ggcgccggcg
    19381 gcggattcgg cggccggctc gccgggcgcc gccgctgtcg cagcctcctc cggagtctcg
    19441 cgtgtgcagg cgccgaggca cagggtgcag gcgatgatcc agggcgcgca atgcgcaagg
    19501 ttcacatttt gcataatcag cctcccgcgc ggcggcgagc aggaggtgaa tataccccgt
    19561 cccctgtatc gtagctgcgc ccgacccgga caccctggag cc
//
LOCUS       DAOQVY010000041        18838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_3395_length_18838_cov_5.319544, whole genome shotgun sequence.
ACCESSION   DAOQVY010000041 DAOQVY010000000
VERSION     DAOQVY010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18838
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3395_length_18838_cov_5.319544"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="PKN91_05660"
     CDS             complement(<1..1029)
                     /locus_tag="PKN91_05660"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 domain-containing protein"
                     /protein_id="HNS27595.1"
                     /translation="MTRAPLPARLRPLSWTLAAFGGGVLLHVDRLPPWITVVAVGASV
                     TRVLLALRGARLPGFWVRAVVALVLTGAVLASFRTLNGLAAGSALLVAMGSLKLLETR
                     ARRDDYVIIAVALFLLLAACLDRQGLTRVPFYFAQLWITCGAIAVVAAAESLAGVKAP
                     LRLAGRGLLYALPLAVALFLFFPRVQGAFWALPNAGSAVTGLSDEMSPGQIDALTDSD
                     TPAFRVEFAGAAPPPQERYWRGPVLQSFDGVTWRHERSRSFLEPEVDFEGPEYRYRVT
                     LEPHGRNWWFALESVLEAPSRRVLLTMDRRLISAEPVNEAVSYDAVSRPRTRTSARLS
                     TNGYRYALA"
     gene            complement(1026..2003)
                     /locus_tag="PKN91_05665"
     CDS             complement(1026..2003)
                     /locus_tag="PKN91_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNS27596.1"
                     /translation="MAIAARIRHRLDERLAAWVLRRQGADSLPVEIGRRRLYVLPTRA
                     GIGFGVLLFGMLLAGLNYANSLALFLTFLLAGLVLAAMHMCHGNLLGCRIVAARVSPT
                     FAGEEGRLELVVEADGATRHDWSIAIRDGDDVVQGDPVQVNPGVRASMHLHITGARRG
                     VHPLDRVRLTTTWPFGLFRCWTWLHLPCELIVYPAARGSLPLRTGGGRRHGQHERASP
                     GDEEWRGLRAYRDGDVPRRIAWKAYARGGPLLVGEYAAIGSDRHEFDFDSLAPLGLEA
                     RLEQLSRWIVDAEQHGEPYSLRLPQTAIATGRGALHRHRCLTALARVPT"
     gene            complement(2023..2943)
                     /locus_tag="PKN91_05670"
     CDS             complement(2023..2943)
                     /locus_tag="PKN91_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNS27597.1"
                     /translation="MSERLQQAIATLETIILGKSVPLRLAVACLLARGHLLIEDVPGV
                     GKTTLAHALAQVFGLAWQRVQFTSDLLPADIIGVSVFDRNTQQFHFRKGPLFTQLLLA
                     DEVNRASPKAQSALLEAMEERQVSTDVTTYRLPDPFFVVATQNPHEQAGTYALPESQL
                     DRFLMRVRLGYPDALHERELLREGERRELLPQLAPVLDAEHVLALQAAVRNVHVADAL
                     LDYVQMLVTHTRSHPDIRIGLSPRAAQGLLRAAQAWTAISGRHAVLPDDVQAVFVAVA
                     GHRLERRDGVDAADGERLAARVLEAVPVPA"
     gene            complement(2940..5717)
                     /locus_tag="PKN91_05675"
     CDS             complement(2940..5717)
                     /locus_tag="PKN91_05675"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNS27598.1"
                     /translation="MDKVFSPRDIERRLYDRWESNGWFEPSGKGEPFCIVIPPPNVTG
                     TLHMGHAFQDTIMDALTRYHRMRGEDTLWQPGTDHAGIATQMVVERQLAAEGKRRGDM
                     TREAFVERVWQWKAQSGGTISNQLRRLGASVDWTRERFTMDPGLSAAVTEVFVRLHDE
                     GLIYRGKRLVNWDPVLLTAVSDLEVLSEEEDGNLWHLRYPLADGTGHVVVATTRPETM
                     LGDTAVAVHPDDERYTQLIGREIALPPTDRRIPIVADSYVDPAFGSGCVKITPAHDFN
                     DYEIGVRHGLAQLNIFTPHAAINDQAPERFRGLDRFEARKRVVAEFEAAGLLERVEPH
                     KLTVPRGDRTHAVIEPYLTDQWYVRIEPLAAPAIAAVERGDIRFVPENWSKTYFEWMR
                     NIKDWCVSRQLWWGHRIPAWYDESGGIYVGRNEAEVRQRHGLDSTVALRQDSDVLDTW
                     FSSALWPFSTLGWPERTAALAKFYPTSVLVTGFDIIFFWVARMVMFGLKFMDGQPPFR
                     DVYIHGLIRDGEGQKMSKSKGNVLDPLDIVDGIDLESLVAKRTTGLMQPHLAPAIEKA
                     TRKQFPQGIDAYGTDALRFTFAALATQSRDIRFDLGRVSGYRNFCNKLWNAARFVTMM
                     TEADAAADAGAPAEYSFVDRWIRSRLGATIAAVDTAFAEYRFDYAATALYEFTWYEFC
                     DWYLELAKPVLQGESGDAARRGARRTLLEVLEALLRALHPIMPFITEEIWQRIAPLAG
                     REGPTIMIAPWPARADWPADAPAEGEVAWLQRVILALRQVRGEMNIAPSRRIPVLAQG
                     GSAADAERLARHDLYLHRLAGLDSLRALGAGERAPASATALAGDLQLHVPMAGLIDAG
                     AEAERLTKLIAKQEAELKKARGKLANENFVRNAPAEVVAQERERIAAFERDLAQLAPQ
                     LERVRGLMASS"
     gene            complement(5726..6169)
                     /locus_tag="PKN91_05680"
     CDS             complement(5726..6169)
                     /locus_tag="PKN91_05680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HNS27599.1"
                     /translation="MPQVDFYVTEDTDGESRLRLACRVTEKAYQAGHRVLVWCREPGQ
                     LAHFDELLWTFADHSFVPHESLAATDPAPAAPVILSAGIEPAWTPEVLVNLDTAVPPL
                     ALRAARIVEFIDGDPARRATGRERFRQYRDQGITPNTHNIGAASL"
     gene            complement(6177..7667)
                     /locus_tag="PKN91_05685"
     CDS             complement(6177..7667)
                     /locus_tag="PKN91_05685"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNS27600.1"
                     /translation="MRFEVWTKGLTRLGVECLVAGVFEDGELPEPTREIDRASRGRIA
                     ALVKRGDISGRAGETLLVTDLAGLRASRVLLVGQGKRTALSRKGWRKAVQSAVTALTR
                     TRVSSAAFALARPAADELDDYWFARATAELTGAGLYRVNHLKSGKQPKPHALARVLAG
                     PVGTAAAAKRGFTHGEAVALGLHTLRDLGNLPSNICTPSYLANVARKLAKKHPKSVRV
                     RVLDKAAIQRERMGCFLAVTQGSDEPPRFIVLEHRGARRGEAPVVLVGKGITFDTGGI
                     SLKDPGAMDEMKFDMSGAATVLAALTVVAALNLPLNVVGLVPTCENMPSGRAIKPGDI
                     VTSASGQTVEILNTDAEGRLILCDALHYARRFKPAALIDVATLTGACVIALGAHHTGV
                     MANDDALARELVDSGLRADDRAWQLPLTEEYGEQLRSNFADMANVAGRDGGAITAGAF
                     LGKFTKGLKWAHMDIAGTAYLSGTQKGSTGRPISLLSEFLIRRAAR"
     gene            7856..8938
                     /gene="lptF"
                     /locus_tag="PKN91_05690"
     CDS             7856..8938
                     /gene="lptF"
                     /locus_tag="PKN91_05690"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HNS27601.1"
                     /translation="MRRILDRYILREVVLAWLAVTAVLMIILLTNQVAAVLERAADNQ
                     YPRSAVLTLIGLSTVQNLPIVVPVGLLLGVVLALGRLYHESEMTAAQACGVGLARVYL
                     PITALALIVTGLLAWLTLDLAPRAAAHAYQLRSEALRSGQFAPVSPGQFRSFGRGGVV
                     IYAQEAAADGTLERVFIKRAGKGGLEVALAARARHEISPDGSLHTITLYDGARYEGVP
                     GSARFRIVRFAEQVVPVRLPELDPGRRRVEAVPTPILAASSALADRAELHWRIALPVM
                     AITLTLLAVPLSRLRPRQGRYARVWIAVLIYFVYSNLASAARAWIERGIVPEELGLWW
                     VHAALGAVGVAVLAAPAVTARLRYRG"
     gene            8947..10008
                     /gene="lptG"
                     /locus_tag="PKN91_05695"
     CDS             8947..10008
                     /gene="lptG"
                     /locus_tag="PKN91_05695"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HNS27602.1"
                     /translation="MILDRYILRSIIGAVLLVLAVLLALGALFTFIQQQDDIGVGTYH
                     ATDAMLYTLLGMPQQFFEMLPIAALIGSLLGLGSLARGSELTVMRASGVSVARVALSA
                     AIAGLALTVLAVGIGEYLAPPMQKLARQVKAFGKFENVSFSGGGAWVRDGDLLLNVEQ
                     QSGAGEFGGMLVFELTRDNRLAALGRASRARATGEDSWLLEDYAESRFAGDYVNGRHA
                     ATRELTSSIGGSFLGITISQPKDIELRSLWRVVRGFAGVGIDVRPYEFAFWSRVARTV
                     AIVFAVLLAVPFVFGPLRGSGTGARTLVGLLIGIVFFLAQRMLESGAIVFDVSPVLLA
                     CLPTLCLALVSLVLIARTR"
     gene            complement(10015..10995)
                     /locus_tag="PKN91_05700"
     CDS             complement(10015..10995)
                     /locus_tag="PKN91_05700"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNS27603.1"
                     /translation="MMTPRRVAVLAIAALLVMMAALWLGSRRDSATTAHVGSPVLPRL
                     DALLNEVARVRIESKSEAATLVRTGAGWTVEERGFRADPAKLRRLLLGLADLKVIEEK
                     TSDPAKYAQLGVEDPGPEATGTHLLATAAGTEFALIIGKVADSASVYVRVPGSGPSLL
                     AAPRVAADADPKHWLDTALVDLPADRIESVAVTPATGPAWRATRGAATEPLALQGLPK
                     GKVQRSPEIVTPVAALLVNLHAEDVHAAARTGAAAPAPRVVVRTFDGVEIELHGRTEG
                     ERHYIHGTARSTGAASEKEAAELAARIAGFEFELPRYKYDALFRPLADFT"
     gene            complement(10992..12872)
                     /locus_tag="PKN91_05705"
     CDS             complement(10992..12872)
                     /locus_tag="PKN91_05705"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNS27604.1"
                     /translation="MTQRRLLGRGALLALAVLFIGITMLSTWALRGWRLDLTENRLYS
                     VAPGTQRILDSLAEPVNLHFFWSERAAGEYPALKNYGMRVSEFLQELAARSNGKLRLS
                     IVDPQPFSAEEDRAAELGVEGVPIGAGGETFFFGLAGTNSTDGREAIAFFDPSKEEFL
                     EYDVAKLIQQLGRPDKPIVGWLSSLPMGGSFDPMSGQTRDPWYVYAQAAELFTVRDLT
                     PDLTDIDADVDVLVIVHPKSLPLAAQQAIDQFALRGGHLLVFVDPLAEQDPGGASPDN
                     PLAAMAADRSSQLGMLLGAWGVDFNAREIIGDLSHGMSVSMRAGEAPTRHIAILGLDA
                     TSLAADDVVTNGLGSINVATAGYLAPRDGADTLFEPLLQSSALAAPLPVERFAMLMDP
                     ASLQDGFEPTGQRYALAARVTGHVKSAFPDGPGPLRDSAQPLNLIVFADSDLLADYLW
                     LRDVPPSFFGPRAKQAWAHNGDLVWNALDNLAGTADLISVRGRASYSRPFERVDALRR
                     DAEGRLRSKEQELEAQLTATEEKLVALQSQRQDQSGLILTPEQQREIERFEAEKLRIR
                     KELREVRSGLNEDIRGLGNRIKFVNIVLMPAIFALIALFIALWRRKRQAAILLVQRTA
                     RP"
     gene            complement(12880..13614)
                     /locus_tag="PKN91_05710"
     CDS             complement(12880..13614)
                     /locus_tag="PKN91_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNS27605.1"
                     /translation="MGQIVTITKRELAGYFATPVAYVFILIFLLLAAAFTFYLGGFYE
                     RGQADLLPFFNFHPWLYLFLIPAISMRLWAEERKSGSIELLLTLPVTLWQAVLGKFFA
                     AWAFTALALALTCPIWVTVNYLGAPDNGVIVASYLGSLLMAGAFLAIGSCMSALTRNQ
                     VVAFILAVVVCFAFLLAGFPLVLDAFRGWAPQAIVDAVASLSFLTHFEALSKGVLDLR
                     DVLYFALTMGFFLVATAFVLDVRKSE"
     gene            complement(13634..14557)
                     /locus_tag="PKN91_05715"
     CDS             complement(13634..14557)
                     /locus_tag="PKN91_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS27606.1"
                     /translation="MIRTERLTRRYDTLTAVDEVSFEAQPGEVLGFLGPNGAGKTTTL
                     RMIAGFIAPPEGRASICGHDIEREPLAAKRALGYLPEGAPSYGEMSVKAFLEFIADLR
                     GLAGATRRSRLDHVIARLALQDVTAQSIDTLSKGFRRRVGIAQAILHDPPVLILDEPT
                     DGLDPNQKHEVRALINEMAAEKTIVVSTHILEEVEAVCTRAIIIARGRIVADDTPQGL
                     TARSRYHNAVSLQLADAAEYARAQAALAALPQVASVEADERELRLTALPQPGARILTA
                     VTDLANRQSLGLKSLALESGRLDEVFRSITV"
     gene            complement(14711..15496)
                     /locus_tag="PKN91_05720"
     CDS             complement(14711..15496)
                     /locus_tag="PKN91_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27607.1"
                     /translation="MSTTRTHGSPRGEPAPRAPSPDGQQLQLLLARWQQSLELHARYA
                     SLDDEHYQHVQPWPKHERPARWIIDLARQRVDALARLVEQRTREGDRAFIEALEVMGF
                     LANLAGLQGNERFIPLASVEAERRDVLNSTTRTRQAKKADDTTREMPRLATGATRAAA
                     SRASAPRASASRAPASRASASRASAPAAPNRSAAPAVRESQDVIDGRIVDDAIRLLGW
                     GRQWHELGELIARLAGRPAAPEVRRILREHRTRIERQQGFATH"
     gene            complement(15779..16189)
                     /locus_tag="PKN91_05725"
     CDS             complement(15779..16189)
                     /locus_tag="PKN91_05725"
                     /inference="COORDINATES: protein motif:HMM:NF039797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HNS27608.1"
                     /translation="MHKTHLPHTAMALAALLLAPICASGADPKDTLPEPEQVSIPFVD
                     RGGIRDWHVDRDRGLWVQDNHRDWYYAKLLSPCIGLGTATAIGFVTRPPGSFDRFSTI
                     IVPREGSCPVVSLVRSDGPPAKAKRAADARDSEK"
     gene            complement(16307..16382)
                     /locus_tag="PKN91_05730"
     tRNA            complement(16307..16382)
                     /locus_tag="PKN91_05730"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16346..16348),aa:Glu,seq:ctc)
     gene            16509..17918
                     /gene="pcnB"
                     /locus_tag="PKN91_05735"
     CDS             16509..17918
                     /gene="pcnB"
                     /locus_tag="PKN91_05735"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HNS27609.1"
                     /translation="MPRDQHTVSRSQVSPNALRVLYRLKDAGYQAFLVGGCVRDLLIG
                     IVPKDFDVATNALPEEVRRLFRNCRLIGRRFRLAHVFFGQEIIEVATFRAASAPPPGD
                     DPLVDPDAEEGDGAEPEESADFDADIADTEGEDSHVLDEHGRILRDNAYGTIDDDVWR
                     RDFTANALYYNIADFSIWDYVGGVDDVLARRLRVIGDPETRFREDPVRMLRAARFEAK
                     LDFTIDPPVEAAIRELGGLLASAPPARLFDETLKLFLAGSGVRSFTTLRRLGLLDVLF
                     PTVAAYFRQHPDSLVEQLLLRGLANTDQRVRDGKPVTPTYLFALLLYGPIAAAIEALP
                     PNRWHEAGAILDACDRAARDMNARVALPRRFSLGVREMFALQPRLEQPRGRRALRLLE
                     HPRFRAAYDLLLLRAELGLAAPEIAAWWTRLQEAAPEDRDGMADAVSKGGGQAGGARQ
                     GAPRGGRRRRRRGGAKPVA"
     gene            17915..18406
                     /gene="folK"
                     /locus_tag="PKN91_05740"
     CDS             17915..18406
                     /gene="folK"
                     /locus_tag="PKN91_05740"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNS27610.1"
                     /translation="MTHWRPAYIGVGSNLGDSRGLVATAMDRVAALPSVSHFVRAPDY
                     RTRPFGPVAQPDFINSAAGFLTTLGPHELLAALRDIERELGREPARERWGPRAIDLDL
                     LVLGGERLSDPVVTLPHPGIAQRAFVLLPLADIAPSLEVPGVGIVSGLLRAVSTEGAE
                     RLP"
     gene            18403..>18838
                     /locus_tag="PKN91_05745"
     CDS             18403..>18838
                     /locus_tag="PKN91_05745"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNS27611.1"
                     /translation="MTAPAARAVPVPQNYIVVEGPIGVGKTSLARALATHLDAGLVLE
                     RADENPFLERFYRNPRAGAFATQLHFLFQRAQQLAAMNQQDLFAQRRVADYLLAKDRL
                     FACLTLDAAELALYEQVYTRLEIDAPRPDLVIYLQAPVDVLQE"
BASE COUNT         2784 a         6298 c         6770 g         2986 t
ORIGIN      
        1 tgcgagcgca tagcgatacc cgttcgtcga cagccgcgcg ctcgtgcgtg tgcggggccg
       61 ggatacggca tcgtagctca ccgcttcgtt gacgggctcc gccgaaatca ggcgccgatc
      121 catcgtcagc aacacccggc ggctcggcgc ctcgagcacc gattcgagcg cgaaccacca
      181 gttgcggccg tgcggttcga gcgtcacgcg atagcgatac tcgggaccct cgaagtcgac
      241 ctcgggttcg agaaaggacc ggctgcgttc gtgccgccag gtcacaccgt cgaacgattg
      301 cagtacgggc ccgcgccagt agcgctcctg cggaggtggc gccgcgcccg cgaattcgac
      361 gcggaacgcg ggggtgtcgg agtccgtgag cgcatcgatc tgcccggggc tcatttcgtc
      421 cgacagcccg gtgacggcac tgccggcatt gggcagcgcc cagaacgcac cctgcacacg
      481 cgggaagaac aggaacagtg ccacggcgag cggcagcgcg tacagcagtc cgcgaccggc
      541 gaggcgcagc ggcgccttca cgcccgccag cgactcggcc gcggcgacca cggcgattgc
      601 accgcaggtg atccacagtt gcgcgaagta gaagggcacg cgggtgagcc cctggcggtc
      661 gaggcaggcc gcaagcagca ggaacagcgc cacggcgatg atcacgtagt cgtcgcggcg
      721 tgcgcgggtc tcgagcagct tgagcgagcc catggccacg aggagcgcgc tgcccgcggc
      781 gagaccgttc agtgtccgga agctcgcgag tacggcaccc gtgagcacga gcgcgaccac
      841 ggcgcgcacc cagaatcccg gcaggcgtgc gccgcgcagc gcgagcagca cgcgcgtcac
      901 gctcgccccg acggccacga ccgtgatcca cggcggcagg cggtcgacgt gcagcagcac
      961 gccgccgccg aaggccgcga gggtccacga cagcgggcgc agacgcgcgg ggagcggtgc
     1021 gcgcgtcatg tcggcacccg cgcgagtgcc gtgaggcagc gatgccggtg cagggccccg
     1081 cggccggtgg cgatcgcggt ctgcggcagg cgcagcgagt agggctcgcc gtgctgttct
     1141 gcatcgacga tccagcgact caactgctcg aggcgcgcct cgagcccgag cggggcgagg
     1201 ctgtcgaagt cgaactcgtg ccggtcgctg ccgatggccg cgtactcgcc gacgagcagc
     1261 ggaccgccac gcgcataggc tttccacgcg atgcgccgcg gcacgtcgcc gtcgcgatag
     1321 gcgcgcaggc cgcgccattc ctcatcgcca ggcgaggcgc gttcatgctg gccgtgccgt
     1381 cgtccgccgc cggttcgcaa gggcaggctg ccgcgcgccg ccgggtagac gatcagctcg
     1441 cagggcaggt gcagccaggt ccagcagcgg aacaggccga atggccaggt ggtcgtgagg
     1501 cgcacgcgat cgagcgggtg cacgccgcgc cgcgcccccg tgatgtgcag gtgcatcgag
     1561 gcgcgcacgc cgggattcac ctgcaccgga tcgccctgca cgacatcgtc accgtcccgg
     1621 atcgcgatcg accaatcgtg gcgcgtcgcg ccgtccgcct cgacgacgag ttcgaggcgg
     1681 ccttcctcgc cggcgaaggt cggactcacg cgtgcggcca cgatgcggca accgagcagg
     1741 ttgccgtggc acatgtgcat cgcggcgaga acgaggccgg cgagcaggaa ggtcaggaac
     1801 agcgcgaggc tgttggcgta gttgaggccc gctagcagca tgccgaacag cagcacgccg
     1861 aagccgatgc cggcgcgggt gggtagcaca tagagacggc gccggccgat ctccaccggc
     1921 aggctgtcgg caccctggcg ccgcaggacc cacgccgcga ggcgttcatc gagccggtgg
     1981 cgaatgcggg cggcgatggc cacgggagga ttgccgggcg gctcaggcgg gcacgggcac
     2041 ggcttccagg acgcgcgcgg ccagtcgctc gccgtcggcg gcgtccacac cgtcgcgacg
     2101 ctcgagccgg tgacccgcca cggcgacgaa taccgcctgc acgtcgtcgg gcagcaccgc
     2161 atgccggccg ctgatcgcgg tccaggcctg cgcggcgcgc agcaggccct gtgcggcgcg
     2221 cggcgagagg ccgatgcgga tgtccggatg cgagcgggtg tgcgtgacca gcatctgcac
     2281 gtagtcgagc agggcgtcgg cgacgtgcac gttgcgcacg gcggcctgca gcgcaagcac
     2341 atgctcggca tcgagcaccg gcgcgagctg cggcagcagc tcgcggcgct cgccctcgcg
     2401 cagcagctcg cgctcgtgca gcgcatccgg ataaccgagc cgcacgcgca tcaggaagcg
     2461 gtcgagctgc gattcgggca gcgcataggt gccggcttgc tcgtgcggat tctgcgtcgc
     2521 gacgacgaag aacggatcgg gcagccggta ggtggtgaca tcggtgctca cctgccgctc
     2581 ctccatcgcc tcgagcagcg cgctctgcgc cttggggctc gcgcgattga cctcgtcggc
     2641 cagcagcaac tgcgtgaaca gcggcccctt gcggaagtgg aactgctgcg tgttgcggtc
     2701 gaacaccgac acgccgatga tgtccgccgg cagcaggtcg ctcgtgaact gcacgcgctg
     2761 ccacgcgaga ccgaagactt gcgcgagcgc gtgcgcgagc gtggtcttgc cgacacccgg
     2821 cacgtcctcg atgagcaggt ggccgcgcgc aagcaggcag gccacggcga ggcgcagtgg
     2881 cacggacttg ccgaggatga tcgtctcgag cgtggcgatc gcctgctgca gccgctcgct
     2941 catgaggaag ccatcagtcc gcgcacgcgc tcgagctgcg gcgcgagctg cgccaggtcg
     3001 cgctcgaagg ccgcgatgcg ttcacgctcc tgcgccacga cctcggcggg cgcgttgcgc
     3061 acgaagttct cgttcgcgag cttgccgcgc gccttcttca actcggcttc ctgcttcgcg
     3121 atcagcttgg tgagccgctc cgcctcggcg ccggcgtcga tcaggccggc catcggtacg
     3181 tgcagttgca ggtcgccggc gagcgccgtg gccgacgccg gcgcgcgctc gccggcacca
     3241 agcgcgcgca ggctatcgag accggcgagg cggtgcaggt agaggtcgtg gcgcgcgagc
     3301 cgttcggcat ccgcggcgct gccaccctgt gccagcacgg gaatgcggcg cgacggggcg
     3361 atgttcatct caccgcggac ctggcgcagc gccaggatca cgcgctgcag ccacgcgact
     3421 tcgccctcgg cgggcgcgtc ggcgggccag tcggcgcgcg ccggccaggg cgcgatcatg
     3481 atcgtgggtc cttcgcggcc cgcaaggggc gcgatgcgct gccagatctc ctcggtgatg
     3541 aacggcatga tcggatgcag ggcccgcagc agcgcctcga gcacttcgag cagcgtgcgt
     3601 cgcgcgccgc gccgggccgc atcgccggat tcgccctgca gcaccggctt ggcgagctcg
     3661 aggtaccagt cgcagaactc gtaccaggtg aactcgtaca gcgccgtggc ggcgtagtcg
     3721 aagcgatact cggcgaaggc cgtgtcgacc gccgcgatag tggcgccgag gcgcgagcgg
     3781 atccaccgat cgacgaacga gtactcggcc ggcgcgccgg catccgcggc ggcatcggcc
     3841 tcggtcatca tcgtgacgaa gcgcgcggcg ttccacagct tgttgcagaa attgcggtag
     3901 cccgagacgc gcccgaggtc gaagcggatg tcgcggctct gcgtggcgag cgcggcgaac
     3961 gtgaagcgca gcgcgtcggt gccgtaggcg tcgatgccct gcgggaactg cttgcgtgtc
     4021 gccttctcga tggcgggggc gagatgcggc tgcatcagcc cggtggtgcg cttggccacc
     4081 agcgactcga ggtcgatgcc gtcgacgatg tcgagcgggt cgagcacatt gcccttggac
     4141 ttcgacatct tctgaccctc gccgtcgcgg atcaggccat ggatgtagac gtcgcggaag
     4201 ggcggctgtc cgtccatgaa cttgagcccg aacatcacca tgcgcgcgac ccagaagaag
     4261 atgatgtcga agccggtgac cagcacgctg gtcgggtaga acttcgccag cgcagcggtg
     4321 cgttcgggcc agccgagcgt cgagaagggc cacagggccg acgagaacca ggtgtcgagc
     4381 acgtcggagt cctggcgcaa cgccacagtg gagtcgagtc catggcgctg gcgcacctcg
     4441 gcctcgttgc ggccgacgta gatgccgccg gactcgtcgt accaggcggg gatgcgatgg
     4501 ccccaccaca gctggcggct gacacaccag tccttgatgt tgcgcatcca ctcgaagtag
     4561 gtcttcgacc agttctcggg cacgaagcgg atgtcgccgc gctcgaccgc ggcgatggcc
     4621 ggggcggcga gcggttcgat gcgcacatac cactggtcgg tgagataggg ctcgatgacc
     4681 gcgtgcgtac gatcgccgcg cggcacggtc agcttgtgcg gctcgacacg ctcgagcagg
     4741 cccgccgcct cgaactcggc cacgacgcgc ttgcgtgcct cgaagcgatc gaggccgcgg
     4801 aagcgctccg gcgcctggtc gttgatggcc gcgtgcggcg tgaagatgtt gagttgcgcc
     4861 agtccgtggc gcacgccgat ttcgtagtcg ttgaaatcgt gcgcgggtgt gatcttcacg
     4921 cagccggacc cgaaggccgg atcgacgtag ctgtcggcga cgatcgggat gcgccggtcg
     4981 gtgggcggca gcgcgatctc gcgaccgatg agctgcgtgt agcgctcgtc gtcgggatgc
     5041 acggcgactg ccgtgtcgcc gagcatggtc tccgggcgcg tggtcgcaac gaccacgtgg
     5101 cccgtgccgt cggcgagcgg atagcgcagg tgccacaggt tgccgtcttc ctcctccgac
     5161 agcacttcga ggtcggaaac cgcggtcagt agcaccgggt cccagttgac cagccgtttg
     5221 ccgcggtaga tgagcccctc gtcgtgcagg cgcacgaaca cttcggtgac ggcggccgac
     5281 aggcccgggt ccatcgtgaa gcgctcgcgc gtccagtcga ccgaggcgcc gaggcgacgc
     5341 agctggttcg agatggtccc gccggattgt gccttccact gccagacgcg ctcgacgaag
     5401 gcttcgcgcg tcatgtcgcc gcggcgcttg ccctcggcgg ccagctggcg ctcgaccacc
     5461 atctgcgtgg cgatgccggc gtggtcggtg ccgggctgcc acagcgtgtc ctcgccccgc
     5521 atgcggtggt agcgcgtgag cgcgtccatg atcgtgtcct ggaacgcatg ccccatgtgc
     5581 agcgtgccgg tgacgttcgg cggcggaatg acgatgcaga acggctcgcc cttgcccgaa
     5641 ggctcgaacc agccattgga ttcccagcga tcgtagaggc gccgttcgat gtcgcgcggc
     5701 gagaagacct tatccatccg ccaaattata gcgaggcggc gccgatgtta tgggtgttcg
     5761 gagtgatgcc ctggtcacga tactggcgaa aacgctcgcg gcccgtggcg cgccgcgccg
     5821 gatcaccgtc gatgaactcc acgatgcgcg ccgcgcgcag tgcgagcggg ggaaccgcgg
     5881 tgtcgagatt gacgagcacc tcgggcgtcc aggccggctc gatgcccgca gagaggatca
     5941 ccggcgccgc gggggccgga tcggtcgcgg cgagcgattc gtgcggcacg aagctgtggt
     6001 cggcaaaggt ccacagcagc tcgtcgaagt gcgcgagctg cccgggctcg cggcaccaga
     6061 cgagcacgcg gtggcccgcc tggtaggcct tctcggtcac ccggcaggcg agccgcaggc
     6121 gcgattcgcc gtcggtgtct tcggtgacgt agaagtcgac ctggggcaag gccgtatcag
     6181 cgcgcggcgc ggcggatcag gaactccgac agcaggctga tggggcggcc ggtgctgcct
     6241 ttctgggtcc ctgacaggta ggccgtgccg gctatgtcca tatgggccca cttgaggccc
     6301 ttggtgaact tgccgaggaa agcgcccgcg gtgatggcgc cgccgtcgcg gccggcgaca
     6361 ttcgccatgt cggcgaaatt gctgcgcaac tgctcgccgt attcctcggt gagcggcagc
     6421 tgccaggcac ggtcgtcggc gcgcaggccg ctatcgacca gttcgcgcgc cagcgcgtcg
     6481 tcgttggcca tgacgcccgt gtgatgggcg ccgagcgcga tcacgcaagc gccggtgagc
     6541 gtggcgacgt cgatgagggc tgcgggcttg aaacggcgcg cgtagtgcag ggcatcgcac
     6601 aggatcaggc gcccttcggc gtcggtgttc aggatttcga cggtctggcc ggaggcgctg
     6661 gtcacgatgt cgccgggctt gatcgcgcgg ccgctgggca tgttctcgca ggtcggcacg
     6721 agcccgacga cattgagcgg caggttgagc gccgcgacga cggtgagggc ggcgagcacg
     6781 gtggcggcgc cgctcatgtc gaacttcatc tcgtccatcg ccccggggtc cttcagggaa
     6841 atgccgcccg tgtcgaaggt gatgcccttg ccgacgagca cgaccggcgc ctcgccgcgc
     6901 ctggcgccgc ggtgttcgag cacgatgaag cgtggcggct cgtcgctgcc ctgcgtgacg
     6961 gcaaggaagc agcccatgcg ctcgcgctgg atcgcggcct tgtccagcac tcgcacgcgc
     7021 accgatttcg ggtgcttctt cgcgagcttg cgcgcgacat tggcgaggta gctcggcgtg
     7081 cagatattcg acggcaggtt gccgagatcg cgcagggtgt gcaggccgag cgcgacggct
     7141 tcgccgtggg tgaacccgcg cttcgccgcg gcggcggtgc cgaccgggcc ggcgagcacg
     7201 cgcgcgagcg cgtggggctt cggctgcttg ccggatttca ggtggttgac gcggtagagg
     7261 cccgcgccgg tgagttccgc ggtggcgcgc gcgaaccagt agtcgtcgag ttcgtcggca
     7321 gctgggcggg cgagtgcgaa cgccgcgctc gagacccggg tgcgggtgag cgccgtgacg
     7381 gcgctctgca ccgccttgcg ccagcctttg cgactgagcg ccgtgcgctt gccctggccg
     7441 accagcagca cgcgcgatgc gcgcaggccg gcgaggtcgg tgacgagcag ggtctcgcct
     7501 gcccggccgg aaatatcgcc ccgttttacg agcgctgcga tccggccgcg gctcgcgcgg
     7561 tcgatctcgc gggttggctc cggcagttcg ccgtcctcga aaacgccggc aaccaggcat
     7621 tcgacaccca gccgcgtcag ccctttagtc cacacctcaa agcgcattat tgggtctccc
     7681 gacaacggct gtgccgcgcc gcgtccatgc cgttgtgaca cgaagcggtc tgatttacct
     7741 taggtccggt tcgccgtccc atcccaattc aagtgattta gcatcgtttc tgatcttgct
     7801 tcggggtgta taagcggttg gcgctagtgt aaccgaactc ttgcggggcc gtgctttgcg
     7861 acggattctg gaccgttaca tcctgcggga ggtcgtgctg gcgtggctcg cggtcaccgc
     7921 cgtgctgatg atcatcctgc tgacaaacca ggtcgccgcg gtgctcgagc gcgcggccga
     7981 caaccagtat ccgcgctcgg cggtgctgac cctcatcggc ctctcgaccg tgcagaacct
     8041 gccgatcgtc gtgccggtcg ggctgctgct cggcgtcgtg ctcgccctcg gccgcctgta
     8101 tcacgaaagt gagatgaccg ccgcccaggc ctgcggtgtg gggcttgccc gcgtctatct
     8161 gccgatcacg gcgctcgccc tcatcgttac gggcctgctc gcctggctga cgctcgatct
     8221 cgcgccgcgc gctgcggccc atgcctacca gctgcgcagc gaggcgctgc gttccggcca
     8281 gttcgcgccg gtgtccccgg gccagttccg cagcttcggc agaggtggcg tcgtcatcta
     8341 cgcgcaggaa gcggcggccg acggcacgct cgagcgcgta ttcatcaagc gcgccggcaa
     8401 gggcgggctc gaggtggcgc tggcggcgcg ggcacgccac gagatctcgc ccgatggctc
     8461 gctgcacacg atcacgctct acgatggcgc gcggtatgaa ggcgtgccgg gcagtgcgcg
     8521 ctttcgcatc gtgcgcttcg ccgagcaggt ggtgccggtg cgcctgcccg agctcgaccc
     8581 tggccggcgc cgggtcgagg ccgtgccgac cccgatcctc gcggcgagct cggcgcttgc
     8641 cgatcgcgcc gagttgcact ggcggatcgc gctgcccgtg atggcgatca cgctgaccct
     8701 gctcgccgtg ccgctcagcc gcctgcggcc acgccagggc cgctatgcgc gcgtgtggat
     8761 cgcggtgctg atctacttcg tctactcgaa cctggcgtcg gccgcccgcg cgtggatcga
     8821 gcgcggcatc gttcccgagg aactcggtct gtggtgggtg cacgccgcgc tgggtgcggt
     8881 gggtgtcgcg gtcctggcgg cgcccgccgt gacggcgcgt ctgcggtacc ggggctgagg
     8941 ccgatcatga tcctcgaccg ttacatcctg cgatcgatca tcggcgccgt gctgctcgtg
     9001 ctggcggtgc tgctcgcgct cggcgcgctg ttcacgttca tacagcagca ggacgacatc
     9061 ggcgtcggta cctaccacgc caccgatgcg atgctctaca ccctgctcgg catgccgcag
     9121 cagttcttcg agatgctgcc gatcgccgcg ctgatcggct cgctgctcgg gctcggctcg
     9181 ctcgcgcgtg gcagcgagct cactgtcatg cgcgcctcgg gggtgtcggt cgcgcgcgtc
     9241 gcgctgtccg cggcgatcgc gggcctcgcg ttgaccgtgc tcgcggtcgg catcggcgaa
     9301 tacctcgcgc cgccgatgca gaagctggcg cggcaggtga aggccttcgg caagttcgag
     9361 aacgtgagct tctccggcgg cggcgcctgg gtgcgcgatg gcgacctgct gctgaacgtc
     9421 gagcaacagt cgggggcggg cgagttcggc ggcatgctcg tgttcgaact gacacgcgac
     9481 aatcggctcg ccgcactggg ccgcgcgtca cgcgcgcggg ccaccgggga ggacagctgg
     9541 ttgctcgagg actacgccga atcgcgcttt gcgggtgatt acgtcaacgg ccgccacgcc
     9601 gccacacgcg agctgacctc atcgattggc ggcagtttcc tcggcatcac gatcagccag
     9661 ccgaaggaca tcgaactgcg ctcgctgtgg cgggtcgtgc gcggattcgc cggcgtcggc
     9721 atcgatgtac ggccctatga gttcgcgttc tggtcgcgcg tggcgcgcac ggtggcgatc
     9781 gtcttcgcgg tcctgctcgc ggtgccgttc gtgttcgggc cgttgcgcgg ctcggggacc
     9841 ggggcgcgca cgctggtcgg gctgctcatc ggcatcgtgt tcttcctggc gcagcgcatg
     9901 ctcgaaagtg gtgcgatcgt gttcgacgtg tcgccggtgc tgctcgcctg cctgccgacg
     9961 ctgtgcctcg cgctcgtgtc gctcgtgctg atcgccagga cgcgctgagg ggcgctaagt
    10021 gaagtccgcg agcgggcgga agagggcgtc gtacttgtag cgcggcagct cgaattcgaa
    10081 acccgcgatg cgggcggcga gttccgcggc ttccttctcc gatgccgcac ccgtgctacg
    10141 ggccgtgccg tggatgtagt gccgctcacc ctcggtgcga ccgtgcagct cgatctcgac
    10201 gccgtcgaag gtacggacca ccacgcgagg agcgggggcg gcggcgccgg tccgggcggc
    10261 cgcgtgcaca tcttcggcgt gcaggttgac gagcagcgca gcgaccggcg tgacgatctc
    10321 gggcgagcgc tgcaccttgc ccttcggcag accctgcagc gcaaggggct ccgtcgccgc
    10381 accgcgcgtc gctcgccacg cggggccggt cgcgggcgtc acggccacgg actcgatgcg
    10441 gtcggccggc agatcgacga gcgccgtgtc gagccagtgc ttcggatcgg catcggccgc
    10501 gacccgaggt gcggcaagca ggctcggccc gctgccgggc acgcgcacgt agacgctggc
    10561 cgaatcggcg accttgccga tgatgagcgc gaactccgta ccggcggccg tggcgagcag
    10621 gtgagtgccc gtcgcctccg gccccggatc ctcgacaccg agctgcgcgt actttgccgg
    10681 gtcgctcgtc ttctcctcga tcaccttcag gtcggcgagg ccaaggagca ggcgccgcag
    10741 cttggccgga tcggcgcgga aaccgcgctc ctcgacggtc cagcctgcgc cagtgcgcac
    10801 cagtgtggcc gcctcgctct tcgattcgat gcgcacccgc gccacctcgt tcagcagcgc
    10861 gtcgagccgc ggaagcaccg gggagccgac gtgcgcggtg gtggccgagt cgcgccggct
    10921 gcccagccac agcgccgcca tcatcacgag aagtgcggca atcgccagca cggcgaccct
    10981 tcgcggcgtc atcatgggcg cgcggtccgt tgcacgagca atatggccgc ctggcgcttg
    11041 cgccgccaca acgcgatgaa cagggcgatg agcgcgaaga tcgccggcat caggacgatg
    11101 ttcacgaact tgatgcggtt gccgagcccg cggatgtcct cgttgagccc gctgcggacc
    11161 tcgcgcagtt ccttcctgat gcgcaacttc tccgcttcga agcgttcgat ctcgcgttgc
    11221 tgttcgggag tcaggatcag cccggactgg tcctggcgct gcgactgcag cgccacgagc
    11281 ttctcttcgg tcgccgtcag ctgcgcctcg agctcctgtt ccttgctgcg caggcgcccc
    11341 tcggcgtcgc ggcgcagtgc gtcgacccgc tcgaacgggc gcgagtaact cgcgcgcccg
    11401 cgcacgctga tcaggtcggc cgtaccggcg agattgtcga gtgcgttcca cacgagatcg
    11461 ccgttatgcg cccaggcctg cttggcgcgc ggtccgaaga acgagggcgg cacgtcgcgc
    11521 agccaaaggt agtcggcgag cagatcactg tcggcaaaca cgatcaggtt gagcggctgc
    11581 gccgagtccc tgagcgggcc agggccgtcc gggaaggcgc tcttcacgtg gccggtcacg
    11641 cgcgccgcga gcgcatagcg ctgcccggtc ggctcgaagc cgtcctgcag cgaggccgga
    11701 tccatgagca tcgcaaagcg ctcgaccggc agcggagccg cgagtgcgct cgattgcagc
    11761 agcggctcga acagcgtgtc cgcgccgtcg cgcggcgcca ggtatccggc ggtggcgacg
    11821 ttgatggacc cgaggccgtt cgtgaccacg tcatcggccg caaggctcgt cgcgtcgagg
    11881 ccgaggatcg cgatatgacg cgtcggcgcc tcgcccgcgc gcatcgagac ggacatcccg
    11941 tggctcaggt cgccgatgat ctcacgcgca ttgaagtcga cgccccaggc gccgagcagc
    12001 atgccgagct gcgagctgcg gtcggctgcc atggcggcga gcggattgtc gggactcgcg
    12061 ccgcccggat cctgctcggc gagcggatcg acgaacacca gcaggtgccc gccgcgcaac
    12121 gcgaactggt cgatggcctg ctgggccgcg agcggcagcg acttcgggtg cacgatcacg
    12181 agcacgtcga cgtcggcgtc gatgtcggtc aggtcagggg tgagatcgcg cacggtgaac
    12241 agttcggccg cctgcgcata cacgtaccac ggatcgcgcg tctggccgct catcggatcg
    12301 aagctgccgc ccatcggcag gctcgacagc caaccgacga tcggcttgtc ggggcgcccg
    12361 agctgctgga tcagcttcgc gacgtcatat tcgaggaact cttccttcga gggatcgaag
    12421 aaggcgattg cctcgcggcc atcggtcgaa ttggtgccgg cgaggccgaa gaagaaggtt
    12481 tcgccgcccg cgccgatcgg cacgccctcg acgccgagtt cggcggcgcg gtcctcctcg
    12541 gcggagaagg gctgggggtc gacgatcgag aggcgcagct tgccattgct gcgtgccgcc
    12601 agttcctgca ggaattcgct gacgcgcatg ccgtagttct tgagggccgg atattcgccc
    12661 gcggcgcgtt cgctccagaa gaaatgaagg ttcacgggct ccgcgagcga gtcgaggatg
    12721 cgctgcgtgc ccggcgccac ggaatagagc ctgttctcgg tcagatcgag acgccagccg
    12781 cgcagcgccc aggtcgagag catcgtgatg ccgatgaaca gcacggcaag cgccagcaat
    12841 gcgccgcggc cgagcagtct gcgttgtgtc atgggcgact cactccgact tgcgaacgtc
    12901 gagcacgaac gccgtggcga ccaggaaaaa gcccatcgtc agcgcgaagt acaggacgtc
    12961 gcgcaggtcg aggacaccct tggagagcgc ctcgaaatgc gtcaggaacg acaacgaggc
    13021 cacggcgtcg acgatggcct gcggtgccca gccgcggaac gcatccagca ccagcgggaa
    13081 gccggccagc aggaacgcaa agcacacgac caccgcgagg atgaacgcga ccacctggtt
    13141 gcgggtgagc gccgacatgc acgagccgat ggcgaggaac gcgcctgcca tcagcaggct
    13201 gcccaggtag ctcgcgacga tcacgccgtt gtcgggcgcg cccaggtagt tgacggtgac
    13261 ccagatcgga caggtgagcg cgagcgcgag ggcggtgaag gcccaggccg cgaagaactt
    13321 gccgagcacg gcctgccaga gtgtgaccgg cagtgtcagc agcagttcga tcgagcccga
    13381 cttgcgttcc tcggcccaca gccgcatcga gatggccggt atcaggaaca ggtacagcca
    13441 ggggtggaaa ttgaagaacg gcagcaggtc ggcctggccg cgctcgtaga agccgccgag
    13501 gtagaaagtg aacgccgccg ccagcaacag gaagatcagg atgaagacat aggcgaccgg
    13561 cgtggcgaaa tagcccgcca gttcgcgctt cgtgatcgtg acgatttgac ccacgcccgc
    13621 gttccctccc tgctcagacc gtgatgctgc ggaatacctc atcgaggcgt cccgactcga
    13681 gtgccagcga tttcaggccg aggctctgcc ggttcgcgag gtcggtcacc gcggtgagga
    13741 tccgggcacc cggctgcggc agcgcagtca ggcgcagttc gcgctcgtcc gcctcgacgc
    13801 tcgcgacctg gggcagtgcg gcgagggcgg cctgcgcgcg ggcgtattct gcggcgtccg
    13861 cgagctgcaa cgacaccgcg ttgtggtagc gcgagcgtgc cgtgaggccc tgcggcgtgt
    13921 cgtcggccac gatccggccg cgcgcgatga tgatcgcgcg cgtgcagacc gcctcgacct
    13981 cttcgaggat atgggtcgag accacgatcg tcttctcggc cgccatctcg ttgatcaggg
    14041 cgcgcacttc gtgcttctgg ttgggatcga ggccgtcggt cggctcgtcg aggatgagaa
    14101 ccggcgggtc gtgcaggatc gcctgcgcga tgccgacgcg ccggcgaaag cctttcgaca
    14161 gggtatcgat cgattgcgcc gtgacatcct gcagcgcgag gcgcgcgatc acgtgatcga
    14221 gccggctgcg gcgcgtggct cccgccaggc cgcgcagatc ggcgatgaac tcgaggaagg
    14281 ccttgacgct catttcgcca tagctcggcg cgccttcggg caggtaaccg agcgcgcgct
    14341 tggcggcgag cggctcgcgc tcgatgtcgt gaccgcagat gctcgccctg ccttcgggag
    14401 gcgcgatgaa gccggcgatc atccgcagcg tcgtggtctt gccggcgccg ttcggtccga
    14461 gaaagccaag cacctcgccg ggctgggcct cgaagctgac ttcgtcaacg gcggtgagcg
    14521 tgtcgtaacg cctcgtgagt cgttcggtcc tgatcatatg gtgtggtctg gatacgccag
    14581 ccacggaaaa gttccgtggc gcctgcggcg caggggcgca attctgttgg ctggcggcgc
    14641 gcctgtcgag accggcccgc ggggcaggcg ccgcaggcat gtcacgcgag ggaggcgcgc
    14701 gggcggcggg tcagtgcgtc gcgaagccct gctggcgttc gatgcgcgtg cgatgctcgc
    14761 gcaggatgcg gcgcacctcg ggcgcggccg gccgcccggc caggcgcgcg atcagctcgc
    14821 cgagctcgtg ccactggcgg ccccagccga gcaggcggat cgcatcgtcg acgatgcgcc
    14881 cgtcgatcac gtcctggctt tcgcgcacgg cgggagccgc gctacggttg ggagctgccg
    14941 gcgccgaagc gcgggacgcg gacgcgcgag acgcgggggc gcgggacgcg gaagcgcggg
    15001 gcgcggacgc gcgggacgcg gcggcccggg ttgcgccggt tgcgaggcgc ggcatctcgc
    15061 gcgtcgtgtc gtcggctttc ttcgcctggc gcgtgcgggt cgtggagttg agcacgtcgc
    15121 ggcgctcggc ctcgaccgag gcgagcggaa tgaagcgctc gttaccctgc aggccggcga
    15181 gattggcgag gaagcccatc acctcgagcg cttcgataaa ggcgcggtcg ccctcgcggg
    15241 tgcgctgctc gacgagtcgc gccagcgcat cgacacgctg gcgggcgaga tcgatgatcc
    15301 agcgcgcggg gcgctcgtgc ttcggccacg gttgcacgtg ctggtagtgc tcgtcgtcga
    15361 gcgacgcgta gcgcgcatgc agttcgaggc tctgctgcca gcgtgcgagc agcagctgca
    15421 gctgttggcc gtcgggcgag ggagcgcgcg gtgcaggctc gccgcgcggc gagccatgtg
    15481 tgcgggtcgt ggacatgtgc gcactgtagc gacgggtcgc gcgaccgcct gcgatacgtc
    15541 gcacattgcg agtgttccaa ttgcgcgtcg cgagccatat gacatatcgg gagcacaggc
    15601 caggtgactc ctgcgcaggg aattgctcgc gaggcggtca tccggcggac atgcgcggac
    15661 gcgacggatg cacacgcgca ccaggcagcg gaacggccgg catctgacaa gtgacgggca
    15721 atcatcccgg cggcggcgat ctgcggccgc acgtcgctcg ggacttcgtc agccgagcct
    15781 atttttcgct gtcgcgggcg tcggcggcgc gcttcgcctt cgccggtggt ccgtcgctgc
    15841 gcacgagact caccaccggg cagctgccct cgcgcggcac gatgatcgtg ctgaagcggt
    15901 cgaagctgcc gggaggcctg gtgacgaagc cgattgcggt cgcggtgccg agcccgatgc
    15961 agggactcag cagcttcgcg tagtaccagt cgcggtggtt gtcctgcacc cagaggccgc
    16021 ggtcgcggtc gacgtgccag tcgcggatgc caccgcggtc gacaaacgga atcgacacct
    16081 gttcaggctc cggcagcgtg tccttggggt cggcgccgct cgcgcagatc ggtgccagca
    16141 gcagcgcggc gagcgccatc gccgtgtgtg gcaaatgtgt cttgtgcata tgggatgtgc
    16201 gtccagccga tgaaaccact ggctcattcg actgttttgc ggcccggtgg ttccgggcct
    16261 cgtctgcgac cgaggctgcc gttataatcg caaccaattg atttattggc gctccctacg
    16321 ggattcgaac ccgtgttaca gccttgagag ggctatgtcc tgggcctcta gacgaaggga
    16381 gcgttgcgcg gcagtcaggc cttgaggcgc ggtattatag aaatgttgac ctttacctca
    16441 agcggaatgt atcacctttg aattcctctc ccgcgcccct cgaggcgccg gcgcctctcg
    16501 cccggatcgt gcctcgcgac cagcacactg tctcccggtc gcaagtctcc cccaacgcgc
    16561 tgcgcgtgct ctatcgcctg aaggacgccg gctatcaggc ctttctggtc ggcggctgcg
    16621 tacgcgacct gctgatcggc atcgtgccca aggacttcga cgtcgcgacc aacgccctgc
    16681 ccgaggaagt caggcgactg ttccgcaact gccggttgat cggccggcgc ttccggctgg
    16741 cgcatgtgtt cttcggacag gagatcatcg aggtcgcgac ctttcgcgcc gcgagcgcgc
    16801 cgccgccggg cgacgatccg ctggtggatc ccgatgcgga agagggcgac ggggctgagc
    16861 cggaggagtc cgcggacttc gatgcggata tcgccgacac cgagggcgag gactcgcatg
    16921 tgctcgacga gcacggccgc atcctgcgcg acaacgccta cggcacgatc gacgatgacg
    16981 tgtggcggcg cgacttcacc gccaacgcgc tctactacaa catcgccgat ttctcgatct
    17041 gggactacgt cggcggcgtc gacgacgtgc tcgcccggcg actgcgggtc atcggcgatc
    17101 cggagacacg ctttcgcgaa gatccggtgc gcatgctgcg cgcggcgcgt ttcgaggcca
    17161 agctcgactt cacgatcgac ccgccggtgg aggcggcgat ccgggaactc ggtggcctgc
    17221 tcgcctcggc gccgcccgcg cggctgttcg atgagacact gaagctgttc ctcgccggca
    17281 gtggcgtgcg cagcttcacg acgctgcgcc gcctcggcct gctcgatgtc ctgttcccga
    17341 cggttgcggc ctatttcagg cagcacccgg acagcctggt cgagcagttg ctgctgcgag
    17401 gactcgcgaa taccgatcag cgggtgcgcg acggcaagcc ggtgacgccg acttatctct
    17461 tcgcgctgct gctctatggc ccgatcgccg cggcgatcga ggcactgccg ccgaatcgct
    17521 ggcacgaagc cggggcgatc ctcgatgcgt gcgatcgcgc ggcgcgcgac atgaacgccc
    17581 gcgtcgcgct gccgcggcgt ttctcgctcg gcgtgcgcga gatgtttgcg ctgcagccac
    17641 ggctcgagca gcctcgcggc cggcgcgcac tgcgcctgct cgagcatccg cgcttccggg
    17701 cagcctacga cctgctgttg ctgcgcgcgg aactcggact cgccgcgccc gagatcgcgg
    17761 cgtggtggac ccgcctgcag gaggccgcgc cggaagatcg cgacggcatg gcggatgcgg
    17821 tgtcgaaggg cggtggacag gccggtggtg cccgccaggg cgcgccgcgc ggcggacgcc
    17881 gtcgccgccg gcgcggtggc gccaagcccg ttgcatgacg cactggcggc ctgcgtacat
    17941 cggggtgggc agcaacctcg gtgactcccg cgggctggtc gccactgcga tggatcgcgt
    18001 cgccgcgctt ccgtcggttt cccatttcgt gcgcgcgccg gactaccgca cgcggccctt
    18061 cgggccggtc gcacagccgg atttcatcaa ttccgccgcc ggctttctca cgacactcgg
    18121 cccccatgag ttgctcgcgg cactgcgcga catcgagcgc gagcttggcc gcgagccggc
    18181 cagggagcgc tggggcccgc gcgccatcga tctcgacctg cttgtgctgg gcggcgagcg
    18241 cctgagcgac cccgtggtga cgctgccgca tcccggtatc gcgcagcgcg cgttcgtgct
    18301 gctgccgctt gccgacattg cgccctcgct cgaggtgccg ggagtcggca tcgtctccgg
    18361 gctgctgcgg gcggtgtcca ccgagggcgc ggagcggctg ccatgacggc acccgcggca
    18421 cgggcggtcc cggtcccgca gaattacatc gtggtcgagg gcccgatcgg cgttggcaag
    18481 acgagcctcg cgcgggcgct ggcgacccac ctcgacgccg gtctggtgct cgagcgtgcc
    18541 gacgagaatc cgttcctcga gcgcttctac cgcaaccccc gcgcgggcgc ctttgccacg
    18601 cagctgcatt ttcttttcca gcgtgcgcag cagctggccg cgatgaacca gcaggacctg
    18661 tttgcgcagc ggcgggtggc cgactacctg ctcgccaagg atcgtctgtt cgcgtgcctg
    18721 acgctcgatg ccgcggagct cgcgctctac gagcaggttt atacgcgcct cgagatcgat
    18781 gcgccgaggc cggacctcgt gatctacctg caggcgccgg tcgacgtgct gcaggagc
//
LOCUS       DAOQVY010000042        18605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_3449_length_18605_cov_5.571968, whole genome shotgun sequence.
ACCESSION   DAOQVY010000042 DAOQVY010000000
VERSION     DAOQVY010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18605
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3449_length_18605_cov_5.571968"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..1432
                     /locus_tag="PKN91_05750"
     CDS             203..1432
                     /locus_tag="PKN91_05750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HNS27612.1"
                     /translation="MQNESFSVLLELAPTRARAGWPQGVDPGFEPVPLARGAMREAGA
                     GAAGPTIVVRADIRRADLARLRADRDVVAVWSDPRVAAVQVEGSAAIDCTPRVPTGAA
                     SDVARALGADRVWQASGYRGDRTVIGIVDGGIDGARFPVVGGWSPDPAAPPGSARVAW
                     EGHGNMCAFDAAIACPQARFHDYSIGKTAPGAGIAGLLSSALQSFHRALVQHAADGTP
                     QVLSNSWGLYQEAWDPFPPGDPSNYTRNPDHVFTRKLIELLDAGILVAFAAGNCGGAC
                     ADARCGADQGPGRGIWGAGGHARSICVGAVNLRGEWIGYSSEGPAALSPEKPDVCGFS
                     HFEGFFASDNGTSAACPVVAGVLGLFAGARAGVSQDRAREALRATARRGAAGDAWNPR
                     FGAGVIDAEAAWAVLAA"
     gene            1429..1677
                     /locus_tag="PKN91_05755"
     CDS             1429..1677
                     /locus_tag="PKN91_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27613.1"
                     /translation="MMRTVLLEFSADSSATAIASRLEAAGIARLPGFDDVPLRGTGEN
                     PGTVVVTVLVPAGIRLERLYDIPGVVRVTQDVPLGPMP"
     gene            1747..2349
                     /locus_tag="PKN91_05760"
     CDS             1747..2349
                     /locus_tag="PKN91_05760"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNS27614.1"
                     /translation="MSVTLPPLPYPLEALEPHVSARTLSVHHGKHHAAYVDRTIALVK
                     GTALESASLEEIVRAEAGKPGPLFNSAAQAWNHDFLWKSMRPGGGGDAVGPIAQRIEK
                     DFGSHDAFRQQFSKAAADQFGSGWAWLVLDGETLRIVATSNAATPLTGPQVPLLTLDV
                     WEHAYYLDYQQRRADYIAAFLAHLANWDFANANLAARASR"
     gene            2656..5532
                     /locus_tag="PKN91_05765"
     CDS             2656..5532
                     /locus_tag="PKN91_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNS27615.1"
                     /translation="MHGKKVARQQDRVQVFVRLDEPAVAELNAESIARTGEYAAPAAQ
                     RAQAQRVSQQQARFRPALESVGAEILSAQRVGANGFRVSVRPSEIPTLRSLPGVRSVG
                     QVEIHTLDNAESVPWTGAPAVWAALGTGEGVSIGVIDSGIDYTHANFGGSGDPADYAA
                     NNRNIVEPGSFPTAKVVGGYDFAGPTYNANVTGSVPAPDGDPLDRGGHGSHVAGSAAG
                     VGVPGSIGPGVAPGASLYALKVFGDAGGSTSLTSLAIEWAMDPNADGDMSDHLDVINM
                     SLGSPLGSPDDPSAISTNNAAAVGIIVATSAGNEGNTPYVTGAPGVASAAISTAANIP
                     GGRLSSRFTVTAPADLAGVYPSLEGAGPVTFGEVGPISGNLVPATPIDGCAALTNAGD
                     IAGNVALIRRGTCGFVVKYEMAQAAGATAIVVFNDGLDATRVDPIVMGGLGATVTIPG
                     LMISSTVGDVLAGTAGVTVTLDAFADPADDDQIGVFSSRGPGHGGSTFKPDLSAPGVA
                     IISTGVATGTGSLNLQGTSMASPHVAGAAALLRQKHPNLDQSAIKALLQNSTVDSNAT
                     GDTDLARQGVGSLRIDRAVALTSYAAPGGVSFGRLNPITSLNVKEDVRLTDLSGKRRT
                     FGVTHVPNQTYPGVTVECPRHVKVNGKGSTKFKISLKFDPRVAWRNDVYDDAIVSQTE
                     VDGWCVLDDGKDSLRVGYMAVVDPASAVLVLPDHGFGGAKLLNIGPSIGWAEGFTLAK
                     LGGEEQDRTYGSIAATGFRLADPTSPMYGGLRVLELGFAMERSFEHLSNLYFLLEIDA
                     DSDGVPDAFLEGTDMSDYFDVDPGQFITLQFNASGSGFADWLANSWDYNDRTLILPFT
                     LSTDPSFPGFVPEKFDYVLSVTARGGVTDVQRGTIDLSQAIVPDVNSFGVEPFDHVDV
                     DFTGPNGTTLWLLQNNVSAAQPALALHYAHKNSSKGKKTHRRGR"
     gene            complement(5587..6117)
                     /locus_tag="PKN91_05770"
     CDS             complement(5587..6117)
                     /locus_tag="PKN91_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27616.1"
                     /translation="MQRAREALARHLDVAVDEVTVTGAEARTWSDSSMGCGQPGSMAA
                     TVITEGFAVSATAGGRPYAVHVSGENVVVCGPATLTRRDRSPAVRAEGIDVLMERARE
                     DLAQRLKVEPAAIRLDGVRGQQWPDSGLGCPQPGETIEPGPVEGLVIALRYRGRLYLY
                     HTDRHTLRPCPAIEAE"
     gene            complement(6219..6764)
                     /locus_tag="PKN91_05775"
     CDS             complement(6219..6764)
                     /locus_tag="PKN91_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNS27617.1"
                     /translation="MGAAAGASAADDISYSYVDAGWVQTDLDDVNEDGDGFLLRGSVA
                     FAEHWFASVGYRQISFDVAGNDIDIDLVDVGLGGHLALSNTVDGVARLSYVDASADGP
                     FGGSFDDNGYAVSVGVRARTGAQVELDAFLDYTDLDDSGDDTAARLGARYYFTPAWAV
                     GAEVSFSDDQTDFGIYGRFSF"
     gene            complement(6977..7051)
                     /locus_tag="PKN91_05780"
     tRNA            complement(6977..7051)
                     /locus_tag="PKN91_05780"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7017..7019),aa:Asn,seq:gtt)
     gene            complement(7099..7479)
                     /locus_tag="PKN91_05785"
     CDS             complement(7099..7479)
                     /locus_tag="PKN91_05785"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HNS27618.1"
                     /translation="MGWRVLADLVVALHFAFILFVSAGGFLVWRWPQLVWLHALALVW
                     GFWVLATGAYCPLTPLENAWRVRAGELGYTSGFIEHYITPVIYPAGLTRGFQATLAAG
                     LIVLNVFAYGGLWRRRRRRRAPIA"
     gene            complement(7484..8686)
                     /locus_tag="PKN91_05790"
     CDS             complement(7484..8686)
                     /locus_tag="PKN91_05790"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNS27619.1"
                     /translation="MALPVSRRVQRVKPSPTLAVTARAAKLKAEGKDIVSLGAGEPDF
                     DTPAHIAAAGVEAIRRGHTRYTNVDGINELKDAIIAKFRRDNQIEYERSQILVSSGAK
                     QTIYNLCMAVLDAGDEAIIPAPYWVSYPDMVMLADGLPVMPFAGASQGYKISPRQLEV
                     AITPATRLVLLNSPCNPTGAAYTRAELQALGEVLLQHPRIVIGTDDMYEKIYWADEPF
                     CSLLTAVPELYDRTVTINGCSKAYAMTGWRIGYCGGPKEIVSAMSTIQGQSTSNASSI
                     SQRAAVVALSGDQASVGEMCAQYRKRHDYVVAGLNSLPGISCLPGAGTFYAFADVSAA
                     MAATGCRDDNAFAELLLNEGGVALVAGSSFGAPGHVRISFACSMETLEKAIDRIGRVL
                     AGAAVKTA"
     gene            8762..10804
                     /gene="uvrB"
                     /locus_tag="PKN91_05795"
     CDS             8762..10804
                     /gene="uvrB"
                     /locus_tag="PKN91_05795"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNS27620.1"
                     /translation="MSATGKAAGPVTAPFRLRADYGPAGDQPQAIDKLVAGLADGLAH
                     QTLLGVTGSGKTYTMACVIERVQRPTLVVAHNKTLAAQLYGEFREYFPDNAVEYFVSY
                     YDYYQPEAYVPSSDTYIEKDASINEHIEQMRLSATKAMLERRDTIIVATVSSIYGLGD
                     PQAYFAMVLHLKRGDRLDQRQLLRRLADMQYTRNELDLQAGTYRVRGDVIDIFPAESD
                     RAAVRVELFDDTVESLAEFDPLTGEVSRKLPRYTIYPGSHYVTPRERLIGAIDLIRED
                     LRLRLRELREADKLVEAQRLEQRTMFDMEMMKEVGYCAGIENYSRYLSGREPGEPPPC
                     LYDYLPEDALLIVDESHQTIPQLGAMYRGDRSRKETLVEYGFRLPSALDNRPLKFEEW
                     EALAPQMIFVSATPGDYEARMSSQVVELLVRPTGLVDPEVEVRPVRTQVDDVLSEIRL
                     RAARNERVLITTLTKRMAEDLTEYLHEHGVKVRYLHADVETVERTEIIRDLRLGRFDV
                     LVGINLLREGLDMPEVSLVAILDADKEGFLRSDNSLIQTIGRAARHINGRAILYADRE
                     TRSMQRAIEETNRRRAKQQAYNAEHGITPRGIEKAVADIMEGARSEAPRPRGRAVRDD
                     RTTALRPEQVVRRIKQLEAEMFRKARSLEFEEAARLRDQIEHLKKAELGLPDAAAG"
     gene            10884..10958
                     /locus_tag="PKN91_05800"
     tRNA            10884..10958
                     /locus_tag="PKN91_05800"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10916..10918,aa:Val,seq:gac)
     gene            10986..11315
                     /locus_tag="PKN91_05805"
     CDS             10986..11315
                     /locus_tag="PKN91_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNS27621.1"
                     /translation="MIGSAGVQEALQLRREELLARANRARDDAAHRRQAVSPDFSEQA
                     AERQNDDVLRGIESAAREEIAQIDSALDRLQRGEYGICVECGREIESARLLAVPYTDR
                     CLAHAGT"
     gene            11322..13250
                     /gene="thrS"
                     /locus_tag="PKN91_05810"
     CDS             11322..13250
                     /gene="thrS"
                     /locus_tag="PKN91_05810"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNS27622.1"
                     /translation="MPVITLPDGSQRSYDAPVSIDAIAASIGAGLRKAALAGRVDGKL
                     VDTSFVLDRDAKVSIVTSKDTEGLEILRHSTAHLLAQAVKQLYPEAQVTIGPVIEDGF
                     YYDFAFSRPFTPEDLAAIEQRMKELAEQDEKVTRRVMSRDDAVRYFQDIGEYYKAEII
                     ASIPANEEISLYGQGDWVDLCRGPHVPSTGKLKVFKLMKVAGAYWRGDSRNEMLQRIY
                     GTAWADKKDLDAYLHRLEEAEKRDHRRIGRELDLFHMQEEAPGAVFWHPKGWSIFQGL
                     IQYMREKQRAAGYEEVNAPEIMDASLWQQSGHLEKFGENMFLTQTPDERLYAIKPMNC
                     PGHVQIFKQGIRSHHDLPLRFAEFGKVHRYEPSGALHGLMRVRAFTQDDAHIFITEDM
                     ITEESVRGIELLLSIYRDFGFDDVKVKYSDRPEKRVGSDEVWDRAERALIDATRAAGI
                     EYTLNPGEGAFYGPKLEFVLQDAIGRDWQCGTWQVDLNLPGRLGAHYIDEHSARRTPV
                     MLHRAIFGSLERFFAILLEHHAGRLPAWLAPVQAVVCTITDRQDEYARKVADSLKNQG
                     FRVESDLRNEKVGFKIRAHTLQRVPYLLVAGDKEVAANLLSVRTRGGKDLGTMSPETF
                     AERLRDELESRGRRTLED"
     gene            13254..13775
                     /gene="infC"
                     /locus_tag="PKN91_05815"
     CDS             13254..13775
                     /gene="infC"
                     /locus_tag="PKN91_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNS27623.1"
                     /translation="MATASKRVRRNDDISAPQVRVIAADGSQAGVMSRYDALQMAMAA
                     GLDLVEVSPTAEPPVVRVMDYGKFLFEQNKKAHSAKKKQKQIQVKEVKFRPGTDENDY
                     QVKLRNLVRFLTEGDKAKVTLRFRGREMAHQEIGRKLLDRVVNDLAGIGTVEQNPLME
                     GRQMVMVFAPKKK"
     gene            13853..14056
                     /gene="rpmI"
                     /locus_tag="PKN91_05820"
     CDS             13853..14056
                     /gene="rpmI"
                     /locus_tag="PKN91_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNS27624.1"
                     /translation="MPKLKTNRAAAKRFRKTAKGFKSYAAGRRHNLGHKSAKVKRHAR
                     SGGSSLVHESDVGRLAQLLPNHK"
     gene            14076..14438
                     /gene="rplT"
                     /locus_tag="PKN91_05825"
     CDS             14076..14438
                     /gene="rplT"
                     /locus_tag="PKN91_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNS27625.1"
                     /translation="MARVKRGVTAGRRHKKVLGKAKGYYNARRKVYRTAKQAVIKAGQ
                     YAYRDRRTKKREFRALWITRINAAARLYDISYSQLVNGLRKAGIEIDRKMLADIAVHD
                     APAFEAIAKQAKAALAAA"
     gene            14445..15467
                     /gene="pheS"
                     /locus_tag="PKN91_05830"
     CDS             14445..15467
                     /gene="pheS"
                     /locus_tag="PKN91_05830"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004979194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNS27626.1"
                     /translation="MPDLVRLTEQALAAIGASDDLSSLDAVRVHWLGKKGVLTEELKA
                     LGKLPAAERPAAGQRINEAKTAVQAAIESRREALERRAVAQALEAGRIDVTLPGRGER
                     RGSLHPITLARQRIEKLFRNAGFAVETGPEIEDDFHNFAALNIPADHPARAMHDTFYI
                     PDGRVLRTHTSPVQVRTMLSQEPPLALIAPGRVYRWDNDLTHSPMFHQVEGLVVGENV
                     SFANMKAALHDFLRGFFERELVLRLRPSYFPFTEPSAEVDISCVFCGGQGCRVCKHTG
                     WLEVAGCGMVHPNVFTACGIDAERWTGYAFGFGIERLAMLRYDVNDLRLFFDNDLRFL
                     RQFASA"
     gene            15469..17850
                     /gene="pheT"
                     /locus_tag="PKN91_05835"
     CDS             15469..17850
                     /gene="pheT"
                     /locus_tag="PKN91_05835"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNS27627.1"
                     /translation="MKLPLSWLKEWVDVGADAHTIGTRLTLAGLELEGVSQAAPPFSG
                     VVVAEIVTAARHPEADKLQVCSVAFGEEAPVQIVCGAANARAGLRVALATIGAVLPGE
                     LRIKRAKLRGVESFGMLCSAKELGLAETSDGILELPGDAPVGCDLREWLALDDEIIEL
                     NVTPNRGDAMSVRGVAREVAAVFGEGRQITGPALQVVPPASDERRAVHLDWPQGAPRF
                     AGRVIRGIDNTRATPLWLRERLRRAGQRSISPVVDVTNYVLLELGQPMHAYDLAKLQG
                     DVRARAAREGESLTLLDGNTIELAADHLVIADDTGAVGLAGIMGGERTAVSAATVDVF
                     LESAWFSPQIIAGRGRRHGLHTDASQRFERGVDPAGQVEAIERATVLLTQIAGGRPGP
                     TQRVERPEFLPARAPVPLRRARLSRLLGQAPADAAVEGHLRALGMIVERNAEGWAATP
                     PGHRFDITIEADLIEEVARLQGLDAIAGAPLHAAQRIAARPSSEPDEARALELLAARG
                     YHEAINFAFVDAALQSRLFPGAQAAALANPIASDLAVMRVSLWPGLLRALRENQRHQQ
                     ERVRLVEHGTVFLVDGAATREVDMIAGVASGTRMPEQWGSAKATVDFHDVKGDIEALL
                     ATTGDSARIEFVAGAGACLHPGRSAGILRGGRRVGSLGELHPELVRALDLTYVPVLFE
                     LEFAALATGMPRFQEVSRFPQVRRDLAIVVDESTPFSAIRERVTLAGSSLLRDLLVFD
                     EYRGPGVESGRKSIALGLIFQDNSRTLTDQDADRIVAAIVADLAGALNAKTRE"
     gene            17853..18152
                     /gene="ihfA"
                     /locus_tag="PKN91_05840"
     CDS             17853..18152
                     /gene="ihfA"
                     /locus_tag="PKN91_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HNS27628.1"
                     /translation="MALTKAEMAEALFNQLGLNKREARELVDLFFEEVRAALSNGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEEIPITARRVVTFRPGQKLKARVEAYAGSKE"
     gene            18133..18489
                     /locus_tag="PKN91_05845"
     CDS             18133..18489
                     /locus_tag="PKN91_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNS27629.1"
                     /translation="MLEAKNNDELPAIPGKRYFTIGEVSELCGVKPHVLRYWEQEFPQ
                     LKPVKRRGNRRYYQRQDVIVIRQIRSLLYEQGFTIGGARNKLTGEEARSDTTQSQQIV
                     RQVRMELEDLLRILRR"
BASE COUNT         3207 a         6093 c         6359 g         2946 t
ORIGIN      
        1 tggccggggt tggcgccacc gaggcgatga aagccaagag agtcagtgcg acgattccgc
       61 gacgaaagtg catgatcatg gattctcctg aagtggacgg ttgcgcagac cgcgcggggc
      121 agtctaggcg caagcgcgca gacccgacaa gccgtgcccg ggacgacacg actgtaggag
      181 aatgaccaca gggagagggc gcatgcagaa cgagagcttc tccgtacttc tggaactcgc
      241 gccgacccgc gcgcgcgccg gctggccgca gggcgtcgat cccggcttcg agccggtgcc
      301 cctcgctcgt ggcgcgatgc gcgaggccgg cgcgggcgcc gccggcccga cgatcgtcgt
      361 gcgcgccgac atccgcaggg ccgacctcgc gcggctacgc gccgatcgcg acgtcgtcgc
      421 cgtctggtcg gatccgcggg tcgcggcggt acaggtcgag ggatccgcgg cgatcgactg
      481 cacgcctcgg gtgccgaccg gcgccgcgag cgacgtggcg cgggcgctcg gcgccgatcg
      541 cgtctggcag gcgagcggtt atcgcggcga tcgcacggtg atcggcatcg ttgacggcgg
      601 catcgacggg gcgcgcttcc cggtggtcgg tggctggtcg cccgatcccg ccgcgccgcc
      661 gggtagcgca cgcgtcgcct gggaggggca cggcaacatg tgcgcgttcg atgcagcgat
      721 cgcctgcccg caggcccgct ttcacgacta ttcgatcggc aagacggcac ccggcgcggg
      781 catcgctggc ctgctctcga gtgcattgca gtcctttcac cgcgcactgg tgcagcatgc
      841 ggcggatggc acgccgcagg tgctgtcgaa cagctggggc ctctatcagg aagcctggga
      901 tccgtttccg ccaggcgatc cgtcgaacta cacacgcaat ccggaccacg tcttcacgcg
      961 caagctcatc gaactgctgg atgccgggat actggtggca tttgccgccg gtaattgcgg
     1021 tggcgcttgc gccgacgcgc gctgcggcgc ggaccagggg ccggggcgcg gcatctgggg
     1081 cgcaggtggc catgcgcgct cgatctgcgt cggcgcagtg aacctgcgcg gcgagtggat
     1141 cggctacagc tcggagggac ccgcggcgct gtcgcccgaa aagcccgacg tatgcggctt
     1201 cagccatttc gagggtttct tcgcgagcga caacggcact tccgcagcat gcccggtggt
     1261 ggcaggtgtg ctcggcctgt tcgcgggcgc gcgcgccggc gtcagccagg atcgcgcccg
     1321 cgaagcgctg cgcgcgacgg cgcggcgggg cgcggcgggt gacgcgtgga atccccgatt
     1381 cggtgcaggc gtcatcgatg ccgaggctgc ctgggctgtg cttgcggcat gatgcgtacc
     1441 gtgctgctgg aattcagtgc tgacagttcg gcgacggcga tcgcctcgcg actcgaggcg
     1501 gccgggatcg cgcgcctgcc gggcttcgac gacgtgccgc tgcgcggcac tggtgagaat
     1561 ccgggcaccg tcgtcgtgac ggtcctcgtg ccggccggca ttcggctcga gcgcctgtac
     1621 gacattccgg gcgtggtgcg cgtgacgcag gacgtgccac tcggaccgat gccatgagac
     1681 atgaagcgcg gcgttgcagc gccattaatg agcgatacta ggaggtcaag tacgaggaga
     1741 ccccccatgt ccgtaactct acccccactg ccatatccgc tcgaagcact cgagccccac
     1801 gtctcggcca ggaccctctc ggtacaccac ggcaaacacc atgccgccta tgtcgacagg
     1861 accatcgcgc tggtgaaggg caccgcgctc gaatccgctt cgctcgaaga aatcgtccgt
     1921 gccgaggcgg gaaagcccgg acccttgttc aactcggcgg cgcaggcctg gaaccatgac
     1981 ttcctctgga agagcatgcg accgggcggg ggcggcgatg cggtcggccc gatcgcccag
     2041 cgcatcgaga aagacttcgg cagccacgac gcctttcgcc agcagttcag caaggcggcc
     2101 gccgaccagt tcggcagcgg ctgggcgtgg ctggtgctcg acggcgaaac gctgcggatc
     2161 gtggccacct cgaatgcagc gacgccgctc accgggcccc aggtgccgct gctgaccctc
     2221 gacgtgtggg aacacgcgta ctacctcgac taccagcagc gccgcgccga ctatatcgcc
     2281 gcgttcctcg ctcacctggc caactgggat ttcgccaacg cgaatctcgc cgcgcgcgcc
     2341 tcacgttagc gtcggcgggc aacggaccag ggacggtccc tgacccgcac agcgtgtgtg
     2401 cctgcgaccg ggcggcatta tgtcgcatgc gcccgtcctc gcgaccggcc ccgccggttg
     2461 aatcctgtca cccgcgggag tagtttcccc gaacttacaa ctacaaccgg ggacagggaa
     2521 atgacacagg ttgtgcgtgc gacgcgatcg aaactcgcgc tcgccttgtt caccgcggct
     2581 ttggttgccg cgcccctctc acaagcgtcc gacaagagca ggcatcgctc cgcggatacc
     2641 agccgtacgt tcaacatgca cggcaagaag gtcgcgcgtc agcaagatcg cgtgcaggta
     2701 ttcgtgcggc tcgacgagcc cgcagtggcg gaactcaacg ccgagagcat cgcccgcacc
     2761 ggcgagtatg ctgcgcccgc cgcgcagcgc gcgcaggcgc agcgcgtttc gcagcagcag
     2821 gcgcgcttcc ggccggcgct ggaaagtgtc ggtgccgaaa tcctctcggc ccagcgcgtc
     2881 ggcgccaacg gctttcgagt cagcgtgagg ccgtcggaga tcccgaccct gcgctcgctg
     2941 ccgggcgtgc gctcggtggg tcaggtcgag atccacacgc tcgacaacgc cgaaagcgtg
     3001 ccgtggaccg gtgcgccggc agtgtgggca gcgctcggga ccggcgaggg cgtatcgatc
     3061 ggtgtcatcg acagcggcat cgattacacc cacgccaatt tcggcggtag cggcgatccg
     3121 gccgattacg cggccaacaa tcgcaacatc gtcgagccgg gcagcttccc cacggcaaag
     3181 gtggtgggcg gctacgattt cgcgggcccg acctacaacg cgaacgtgac cggctccgtg
     3241 ccggcgccgg acggcgatcc gctcgaccgc ggtggccatg gcagccacgt cgccggttct
     3301 gcggcgggcg tcggcgtgcc gggcagcatc ggcccgggcg tcgcgcccgg tgccagcctc
     3361 tatgcactga aggtattcgg cgacgccggt ggcagcacct cgctcacctc gctcgccatc
     3421 gaatgggcga tggatccgaa cgctgacggc gacatgagcg atcacctcga cgtgatcaac
     3481 atgagtctcg gttcgccgct tggttcgccc gacgatccgt cggcgatctc gaccaacaac
     3541 gccgcggcgg tcggtatcat cgtcgcgacc tcggccggca atgaaggcaa tacgccctat
     3601 gtcaccggtg cgccgggtgt cgcgagcgcg gcgatctcca ccgcggcgaa catccccggt
     3661 gggcggctct cttcgcgctt cacggtgacg gcgccggcgg atctcgccgg cgtctatccc
     3721 tcgctcgagg gcgcaggtcc cgtgaccttt ggcgaggttg gacccatctc gggcaacctc
     3781 gtgccggcca cgccgatcga cggctgtgcg gcgctgacca atgcgggcga tatcgccggc
     3841 aacgtggcgc tgatccgtcg cggcacctgc ggcttcgtcg tcaagtacga gatggcgcag
     3901 gctgcggggg ccacggccat cgtcgtcttc aatgacggcc tcgacgcgac gcgcgtcgac
     3961 ccgatcgtga tgggcggcct cggcgccacg gtgacgattc cgggcctgat gatctcgagc
     4021 acggtgggtg acgtgctggc gggcaccgcg ggggtgaccg tgacgctgga tgccttcgcg
     4081 gatccggccg acgacgacca gatcggcgtg ttctcgtcgc gcggtccggg gcatggtggc
     4141 tcgaccttca agccggacct ctcggcgccg ggtgtcgcga tcatctccac gggcgtggcc
     4201 acgggcacgg gcagcctcaa cctgcagggc acgtccatgg cgtcgccgca tgtcgcgggc
     4261 gcggcggccc tgttgcgcca gaagcatccc aacctggacc agagcgccat caaggcgctg
     4321 ctgcagaact cgaccgtcga ctccaatgcg acgggcgaca ccgatcttgc gcggcagggt
     4381 gtcggttcgc tgcgtattga ccgcgcggtt gcgctcacga gctatgccgc gccgggcggc
     4441 gtatcgttcg ggcgcctgaa tccgatcacg agcctcaacg tgaaggagga cgtcaggctc
     4501 accgacctca gcggcaagcg ccgcaccttc ggtgtaaccc atgtgccgaa tcagacctat
     4561 ccgggcgtca cggtcgaatg tccgcgtcac gtcaaggtca acggcaaggg cagcacgaag
     4621 ttcaagatct cgctgaagtt cgatccgcgc gtggcctggc gcaacgacgt ctacgacgac
     4681 gcgatcgtga gccagacgga agtcgacggc tggtgcgtgc tggacgacgg caaggacagc
     4741 ctgcgcgtcg gctacatggc ggtggtcgat ccggcctcgg ccgtgctggt gctgcctgac
     4801 cacggcttcg gtggcgccaa gctgctgaac atcgggccga gcatcggctg ggcggaaggc
     4861 ttcacgctgg cgaagctcgg cggcgaggag caggaccgca cctatgggtc gatcgccgcg
     4921 accggcttcc ggctggccga cccgacctcg ccgatgtacg gcgggctgcg cgtgctggaa
     4981 ctcggtttcg cgatggagcg ttcgttcgag cacctgtcga acctgtactt cctgctcgaa
     5041 atcgacgccg acagtgacgg tgtgccggat gccttcctcg agggcaccga catgtcggat
     5101 tacttcgacg tcgatccggg ccagttcatc acgctgcagt tcaacgcgtc gggcagtggc
     5161 ttcgcggact ggctcgccaa ttcgtgggac tacaacgacc gcacgctgat cctgccgttc
     5221 acgctctcca ccgatccgtc cttccccggc ttcgtgcccg agaagttcga ctacgtgctg
     5281 agcgtgacgg cgcgtggagg cgtgacggac gtgcagcggg gcacgatcga cctgtcgcag
     5341 gcaatcgtgc cggacgtgaa cagcttcggt gtcgagccgt tcgaccatgt cgatgtggac
     5401 ttcaccggtc ccaacggcac gaccctgtgg ctgttgcaga acaacgtgtc ggcggcgcag
     5461 ccggcgctcg cgctgcacta cgcgcacaag aactcgtcga agggcaagaa gacgcatcgc
     5521 cggggccgtt gatccggcgc gacgagtgat tgacgaatgt gagggccgcc ttcgggcggc
     5581 cctctttcat tccgcctcga tggcggggca gggtcgcagc gtgtggcgat ccgtgtgata
     5641 gaggtagagg cgtccgcgat aacgcagcgc gatcacgagc ccctcgaccg gtcccggctc
     5701 gattgtctcc ccgggctgcg ggcagcccaa gccgctgtcg ggccactgct ggccgcgcac
     5761 gccatcgagg cggatcgcgg ccggctccac cttgagtcgc tgcgcgagat cctctcgtgc
     5821 gcgttccatc aatacatcga tgccctccgc gcgcacggcg ggcgagcggt cgcggcgagt
     5881 aagggttgcc ggtccgcaca cgaccacgtt ttcgccggac acgtgcacgg cgtaggggcg
     5941 cccccccgcg gtggccgaca cggcgaagcc ttcggtgatc acggtcgccg ccatcgagcc
     6001 aggctggccg caacccatgc tcgagtcact ccaggtgcgt gcctcggctc cggtgaccgt
     6061 cacctcgtcg actgccacgt cgagatggcg cgcaagcgcc tccctggcgc gttgcatcgc
     6121 ctgggattgc gccgcgccgg cttcatccgc cggcgcggac tgcgtgcagg cgcccaggag
     6181 gagcgccgcc gcagccatgc ctgcaagtcg cagcttcttc agaacgagaa ccttccgtag
     6241 atcccgaagt cggtctggtc gtcggagaag gatacctcgg cgccgaccgc ccaggcgggc
     6301 gtgaagtaat agcgggcgcc gaggcgcgcc gcggtgtcgt cgcccgagtc gtcgagatcg
     6361 gtgtagtcga ggaaggcgtc gagctcgacc tgcgcgccgg tccgggcacg cacgccgacg
     6421 ctcacggcat agccgttgtc gtcgaaggac ccaccgaatg gaccatccgc cgaggcgtcc
     6481 acgtagctca ggcgcgccac gccgtcgaca gtgttggaga gcgccaggtg gccacccagt
     6541 ccgacatcca cgaggtcgat atcgatgtca ttgcccgcga cgtcgaacga gatctgccgg
     6601 tagccgaccg acgcgaacca gtgctcggcg aaggcgaccg agccgcgcag caggaagccg
     6661 tcgccgtcct cgttgacgtc atcgagatcg gtctggaccc agccggcgtc tacgtaggag
     6721 tagctgatgt cgtcagcggc cgaggcgcca gctgccgcgc ccatcgaaat cgcggcaaaa
     6781 acaccacaaa ttacgggggt gtgtctcatc aggtaaccct tatgtagcag gaaacgcaag
     6841 caatccaata ttgcgacagg tgacccgcga atcggttcca tctgtcacaa aaatgcgaca
     6901 ggggtcacgc gcggcgggcg ccgggagccc tttggctgcg ccgctgctca cgtgagcagg
     6961 atggacaggt gtgatgtggc tccccgggtt ggactcgaac caacgaccaa cggattaaca
     7021 gtccgacgct ctaccgctga gctaccgggg aacgggaggc aagaagggcg cgaattctca
     7081 cggacccggg cagatgcgtc aagcgatggg cgcgcggcgc ctgcgccggc gtcgccacag
     7141 gccgccgtag gcgaacacat tgagcacgat caggccggca gcgagtgtcg cctggaagcc
     7201 gcgcgtgagg ccggccgggt agatcaccgg cgtgatgtag tgttcgatga atccgctcgt
     7261 gtagccgagc tcgcccgccc gtacgcgcca ggcattctcg agcggcgtga gcgggcaata
     7321 cgcaccggtt gcgagcaccc agaagcccca tacgagcgcc agcgcgtgca gccaaacgag
     7381 ctgcggccag cgccagacga gaaagccgcc cgcggagacg aacaggatga acgcgaagtg
     7441 cagcgcgacg acgagatccg ctaggactcg ccagcccacg ccatcaggcg gtcttgaccg
     7501 ccgcccccgc gagtacccga ccgatgcgat cgatggcctt ttcgagcgtc tccatgctgc
     7561 acgcgaacga aatgcgcaca tggcccggtg cgccgaagct cgagccggcc accagcgcca
     7621 cgccaccctc gttcaggagc agttccgcaa acgcgttgtc gtcgcggcag ccggtggccg
     7681 ccatggccgc cgatacgtcg gcgaaggcat agaaggtgcc ggcgccgggc aggcaggaaa
     7741 tgccgggcag cgaattgagc cccgcgacga catagtcgtg ccgcttgcgg tactgggcgc
     7801 acatctcgcc gacgctcgcc tggtcgccgg acagggcgac gaccgcggcg cgctgcgaaa
     7861 tgctcgatgc gttcgaggtg ctctggccct ggatcgtcga catggcgctg acgatttcct
     7921 tcggtccccc gcagtaaccg atgcgccagc ccgtcatcgc gtaggccttt gagcagccgt
     7981 tgatcgtgac ggtgcggtcg tacagttcgg gcaccgcggt gaggaggctg cagaacggct
     8041 cgtcggccca gtagatcttc tcgtacatgt cgtcggtgcc gatgacgatg cgcggatgct
     8101 gcagcagtac ctcgccgagt gcctgcagtt cggcgcgcgt gtacgcggcg ccggtcggat
     8161 tgcacgggct gttgagcagc acgaggcggg ttgccggcgt gatcgccact tcgagctggc
     8221 gcggcgagat cttgtagccc tggctcgcgc ccgcgaacgg catgaccggc aggccgtcgg
     8281 ccagcatcac catgtcggga taggacaccc agtagggtgc cgggatgatg gcctcatcgc
     8341 ccgcgtcgag tacggccatg cacaggttgt agatcgtctg cttggcgccg ctcgagacga
     8401 ggatctgcga gcgctcgtac tcgatctggt tgtcgcggcg gaacttcgcg atgatcgcgt
     8461 ccttcagttc gttgatcccg tcgacattgg tgtagcgcgt gtggccgcgc cggatcgcct
     8521 cgacgcccgc cgcggcgata tgcgccggcg tgtcgaaatc gggctcgccg gcgccgagcg
     8581 acacgatgtc cttgccctcc gccttgagct ttgccgcgcg cgcggtgacg gccagcgtgg
     8641 gggagggctt gacgcgctga acgcgtcgcg agacgggtag tgccacggtt acaacctgca
     8701 ggtgagtggg ggcgggaacg gcgctatatt aactgattgt gccccggatt cgcattcacg
     8761 catgagcgca acaggcaagg ctgccggccc cgttaccgca ccgtttcggc tgcgcgccga
     8821 ctatggcccg gcgggcgatc aaccgcaggc gatcgacaag ctcgtggccg ggctcgccga
     8881 cggcctcgcg caccagacgc tgctcggtgt caccggcagc ggcaagacct acacgatggc
     8941 ctgtgtcatc gagcgggtgc agcgcccgac cctcgtggtc gcgcacaaca agacgctcgc
     9001 ggcgcagctg tacggcgaat tccgcgagta cttcccggac aacgcggtcg agtacttcgt
     9061 ctcctactac gactactacc agcccgaggc ctatgtgccc tcgagcgata cctatatcga
     9121 gaaggatgcc tcgatcaacg agcacatcga gcagatgcgc ctctcggcga ccaaggcgat
     9181 gctcgaacgg cgcgatacga tcatcgtcgc caccgtgtcc tcgatctacg gcctcggcga
     9241 cccgcaggcg tatttcgcga tggtgctgca cctgaagcgc ggcgaccggc tcgaccagcg
     9301 ccagctgctg cgccgccttg cggacatgca atacacgcgc aacgaactcg acctgcaggc
     9361 cggcacctat cgcgtgcgtg gtgacgtgat cgacatcttt cccgccgagt ccgaccgcgc
     9421 tgcggtgcgc gtcgagctgt tcgacgacac ggtggagtcg cttgccgagt tcgatccgct
     9481 gaccggcgag gtgagccgca agctgccgcg ctacaccatc tacccgggct cgcattacgt
     9541 gactccccgc gagcggctga tcggggcgat cgacctgatc cgggaagacc tgcgtctgcg
     9601 acttcgggag ctgcgcgagg ccgacaagct cgtcgaggcg cagcggctcg agcagcgcac
     9661 gatgttcgac atggaaatga tgaaggaggt cggctactgc gcgggcatcg agaactactc
     9721 gcgctacctg tccgggcgcg agcccggcga gccgccgccg tgcctgtacg attacctgcc
     9781 cgaggatgcg ctgctgatcg tcgatgagag ccaccagacg atcccgcagc tcggcgcgat
     9841 gtatcgcggc gaccgttcgc gcaaggaaac cctggtcgag tacggcttcc ggctgccctc
     9901 ggcgctcgac aaccggccgc tcaagttcga ggaatgggaa gcgctcgcgc cgcagatgat
     9961 cttcgtgtcg gcgacacccg gcgactacga agcacggatg tcctcccagg tggtcgaact
    10021 gctcgtacga cccaccggtc tcgtcgatcc cgaggtcgag gtgcgccccg tgcgcacgca
    10081 ggtcgacgac gtgctgtccg agatccgact gcgcgcggcg cgcaacgagc gcgtgctcat
    10141 caccacgttg accaagcgca tggccgagga cctgaccgag tacctgcacg agcacggcgt
    10201 gaaggtgcgc tacctgcacg ccgacgtgga aaccgtcgag cgcacggaga tcatccgcga
    10261 cctgcgcctc ggcaggttcg acgtgctggt cggcatcaac ctgctgcgcg agggcctcga
    10321 catgcccgag gtgtcgctgg ttgcgatcct cgacgccgac aaggagggct tcctgcgctc
    10381 cgacaattcg ctgatccaga cgatcgggcg cgcagcgcgc cacatcaacg ggcgcgcgat
    10441 cctctatgcc gaccgcgaga cccgctcgat gcagcgggcg atcgaggaga ccaatcgcag
    10501 gcgtgcgaaa cagcaggcct acaatgccga gcacggcatc accccgcgcg gcatcgagaa
    10561 ggccgtcgcc gacatcatgg aaggtgcgcg cagcgaggcg ccgcggccgc ggggcagggc
    10621 ggtccgggac gacaggacga ccgcgttgcg gcccgagcag gtcgtgcgcc ggatcaagca
    10681 gctggaggcc gagatgttcc ggaaggcccg cagcctcgaa ttcgaggagg ccgcccggct
    10741 acgcgaccag atcgagcacc tgaagaaagc cgagctcggc ctaccggatg ccgcggccgg
    10801 ttgaggcggg gccctaactt cttgaaatcc cgtggattcg attgcttaca gggtgcgcgt
    10861 tgcggtatct ttcgcgcccc cataggcgcg tagctcagtg gtagagcact tccttgacat
    10921 ggaagtggtc ggtggttcga tcccactcgc gcctaccagt tttttcggat gacacgagta
    10981 tccccatgat cggttcggcc ggcgtccagg aagctctgca gttgcgtcgc gaggagctgc
    11041 tcgcgcgcgc caaccgcgcg cgcgacgacg ctgcgcatcg tcggcaggcg gtcagccccg
    11101 atttctccga acaggcggcc gaacggcaga acgacgacgt gctgcgcggc atcgagtccg
    11161 cggcgcgtga agagatcgca cagatcgaca gcgcgctcga tcgcctgcag cgcggcgagt
    11221 acggcatctg cgtcgagtgc gggcgggaaa tcgaatccgc gcggctcctg gcggttcctt
    11281 acaccgatcg ctgcctcgcc cacgccggaa cctgagtcac agtgccggtc atcacactgc
    11341 ccgacggtag ccagcgcagc tacgacgcgc cggtatcgat cgacgccatc gccgccagca
    11401 tcggcgcggg cctgcgcaag gcggcccttg ccggccgcgt ggacggcaag ctcgtcgaca
    11461 cgagcttcgt gctggaccgc gatgcgaagg tctcgatcgt cacgtcgaag gacaccgaag
    11521 gcctcgagat cctgcgccac tcgaccgcgc acctgctggc gcaggccgtg aagcagctct
    11581 accccgaggc ccaggtcacg atcggcccgg tgatcgaaga cggcttctac tacgacttcg
    11641 ccttttcgcg tccgttcaca ccggaggacc tcgcggcgat cgagcagcgc atgaaggagc
    11701 tcgccgagca ggacgagaag gtcacgcgcc gcgtgatgag ccgcgacgat gcggtccgct
    11761 acttccagga tatcggcgag tactacaagg ccgagatcat cgcgagcatc ccggccaacg
    11821 aggagatcag cctctacggg cagggcgatt gggtcgacct gtgccgcggg ccgcacgtgc
    11881 cctcgaccgg caagctgaag gtcttcaagc tgatgaaggt cgccggcgcc tactggcgcg
    11941 gcgactcgcg caacgagatg ctccagcgca tctacggcac ggcctgggcc gacaagaagg
    12001 acctcgacgc ctacctgcac cgtctcgagg aagccgagaa gcgcgatcat cggcgcatcg
    12061 gccgcgagct cgacctcttc cacatgcagg aggaggcgcc cggcgcggtg ttctggcatc
    12121 cgaaaggctg gtcgatcttc cagggcctga tccagtacat gcgcgagaag cagcgcgccg
    12181 cgggatacga ggaagtgaac gcgccggaga tcatggacgc gtcgctgtgg cagcagtcgg
    12241 gccacctcga gaagttcggc gagaacatgt tcctgacgca gactcccgat gagcgcctgt
    12301 acgcgatcaa gccgatgaac tgcccgggac acgtgcagat cttcaagcag ggaatccgca
    12361 gccaccatga cctgccgctg cgcttcgccg agttcggcaa ggtgcaccgg tacgagccgt
    12421 ccggggcgct gcacggcctg atgcgcgtgc gcgcgttcac gcaggacgac gcgcacatct
    12481 tcatcaccga ggacatgatc accgaggagt cggtgcgggg catcgaactg ctgctgtcga
    12541 tctaccggga cttcggcttc gacgacgtca aggtcaagta ctctgatcgc ccggaaaagc
    12601 gcgtcggcag cgacgaggtg tgggatcgcg ccgagcgggc gctgatcgac gccacccggg
    12661 cggcgggcat cgagtacacc ctcaatccgg gtgagggggc cttctatggc ccgaagctcg
    12721 agttcgtgct ccaggacgcc ataggccggg actggcagtg cggaacctgg caggtggatc
    12781 tcaacctgcc gggccggctc ggggcccact acatcgacga gcacagcgcg aggcgcacgc
    12841 cggtcatgtt gcaccgggcg attttcggct ccctggagcg gtttttcgcc attctgctcg
    12901 agcaccacgc cggtcgcctg ccggcctggc tggcgccggt acaggcggtc gtatgcacca
    12961 tcaccgaccg ccaggacgag tacgcccgga aagtcgcaga ttcgcttaag aatcagggat
    13021 ttcgggtcga aagcgacttg cggaacgaaa aggtgggctt taagattcgc gcccacacgc
    13081 tgcagcgtgt gccatatttg ctggtcgctg gagacaaaga agtcgccgcg aacctgcttt
    13141 ccgtgcgtac ccgtggcggc aaggacttgg gcacaatgtc gccggagacg tttgcagaac
    13201 gactccgcga cgagctcgag agccgcgggc gccgaacttt ggaggattga tttatagcca
    13261 ctgcaagcaa gcgcgtccgg cgcaatgacg atatcagcgc accgcaggtg cgggtaattg
    13321 ctgccgacgg ctcccaggca ggagtcatgt cccggtacga cgcgcttcag atggcaatgg
    13381 ccgctgggct cgatctcgtg gaagtgtcgc ccacggcgga accgccggtg gtgcgggtga
    13441 tggactacgg caaattcctg ttcgaacaga acaagaaggc ccattccgcg aagaagaagc
    13501 agaagcagat ccaggtcaag gaagtgaagt ttcgtcccgg gacggacgag aacgattacc
    13561 aggtcaagct gcgcaatctc gtgcgcttcc tgaccgaagg tgacaaggcc aaggtgaccc
    13621 tgaggttccg cggccgtgag atggcgcatc aggagatcgg gcgcaagctc ctcgatcggg
    13681 tggtgaacga cctcgccggc atcggcacgg tcgaacagaa cccgctcatg gaggggcgcc
    13741 agatggtgat ggtcttcgcg cccaaaaaga agtgaccggc gggtaaccgc cggtccgcga
    13801 gcaagcgggc ccgcaagggc tttgcgagct tttgctacaa gaggagatga gcatgcccaa
    13861 gttgaaaacc aaccgggcgg cggccaagcg ttttcgcaag actgcgaaag gcttcaagag
    13921 ctatgcggcc ggccgtcgtc acaacctcgg ccacaagagc gcgaaggtca agcgccacgc
    13981 ccgttcgggt ggcagcagcc tggtgcacga gagcgacgtc ggtcgccttg cgcaactgct
    14041 ccccaaccac aagtaacggc acgaggtaat cagtcatggc acgagtcaag cgtggcgtga
    14101 cggccgggcg ccgtcacaag aaggttctgg gcaaggcgaa gggttactac aacgcccgtc
    14161 gcaaggtcta tcgcaccgcc aagcaggcgg tcatcaaggc ggggcagtat gcgtaccgcg
    14221 accgtcgcac gaagaagcgc gagttccgcg cgctgtggat cacgcgcatc aatgcggcgg
    14281 cgcgcctgta cgacatctcg tacagccagc tcgtgaacgg cctgcgcaag gccggcatcg
    14341 agatcgatcg caagatgctg gcggacatag ccgtgcatga tgcgccggcg ttcgaggcca
    14401 tcgccaagca ggcaaaagcg gcgctcgcgg cggcgtaagt cgccgtgccc gatctcgtcc
    14461 gtctgaccga gcaggcgctc gccgcgatcg gcgcgagcga cgatctgtcg tcgctcgacg
    14521 ccgtacgcgt gcactggctc ggcaagaagg gcgtcctcac cgaggaactg aaggccctcg
    14581 gcaagctgcc ggcggccgag cgcccggcgg ccggccagcg catcaacgag gcgaagacgg
    14641 ccgtgcaggc ggcgatcgaa tcgcgccgcg aggcgctcga gcgcagggcg gtggcgcagg
    14701 cgctcgaggc cgggcgcatc gacgtcacgc tgccagggcg cggcgagcgg cgcggttcgc
    14761 tgcatccgat cacgctcgcg cgccagcgca tcgagaagct gtttcgcaac gcaggcttcg
    14821 ccgtcgaaac cgggccggaa atcgaagacg acttccacaa cttcgcggcg ctcaacattc
    14881 cggccgatca tccggcgcgc gcgatgcacg acacgttcta cattcccgac ggccgcgtgc
    14941 tgcgcaccca cacctcgccg gtgcaggtgc gcaccatgct gtcccaggag ccgccactcg
    15001 cgctgatcgc gccgggacgc gtctatcgct gggacaacga cctgacgcac tcgccgatgt
    15061 tccaccaggt cgagggactg gtggtgggcg agaacgtgag tttcgcgaac atgaaggcgg
    15121 ccctgcacga ttttctgcgg ggcttcttcg agcgcgagct ggtgctgcgg ctgcgtccct
    15181 cgtacttccc gttcaccgag ccttccgccg aggtcgatat ttcctgcgtg ttctgtggcg
    15241 gacagggttg tcgcgtgtgc aagcacaccg gctggctcga ggtggccggc tgcggcatgg
    15301 tgcacccgaa tgtcttcacg gcgtgcggca tcgatgccga gcgctggacc ggttatgcgt
    15361 tcggcttcgg catcgagcgg ctcgccatgc tgcgctatga cgtgaacgat ctgcgtctgt
    15421 tcttcgacaa cgacctgcgc ttcctgcgcc agttcgcgag cgcctgacat gaagctgccg
    15481 ctgtcctggc tcaaggagtg ggtggatgtc ggtgcggacg cgcacacgat cggcacgcgc
    15541 ctcacgctgg cgggcctcga gctcgagggc gtgtcgcagg ccgcgccgcc attctcgggc
    15601 gtcgtggtcg ccgagatcgt gactgccgcc cgtcacccgg aggccgacaa actgcaggtc
    15661 tgcagtgtgg ccttcggcga ggaggctccg gtgcagatcg tctgtggcgc ggccaatgca
    15721 cgcgcgggcc tgcgcgtcgc actcgccaca atcggtgctg tgctgccggg cgagcttcgg
    15781 atcaagcgcg ccaagctgcg gggcgtcgag tccttcggca tgttgtgctc ggcgaaggag
    15841 ctgggcctgg cggagacctc ggacgggatc ctcgagctgc ctggcgatgc gcccgtcggc
    15901 tgcgacctgc gcgaatggct cgcgctcgac gatgagatca tcgagctcaa cgtcacgccc
    15961 aaccgtgggg acgccatgtc ggtgcggggc gtcgcgcgcg aggtggccgc agtgttcggc
    16021 gagggccgcc agatcaccgg cccggcactg caagtcgtgc cgccggccag cgatgagcgc
    16081 cgcgccgtgc acctcgactg gccgcagggc gcaccgcgct ttgccggtcg cgtgattcgc
    16141 gggatcgaca acacgcgggc cacgccactg tggctgcgcg agcgcctgcg ccgcgccggc
    16201 cagcgctcga tcagcccggt ggtcgacgtg acgaactacg tgctgctcga actgggccag
    16261 cccatgcacg cctacgacct tgcgaagctg caaggcgatg tgcgggcacg cgcggcgcgc
    16321 gaaggcgaga gcctgaccct gctcgacggc aacacgatcg agctcgccgc cgatcatctc
    16381 gtgatcgctg acgacaccgg tgccgtgggc ctcgcgggca tcatgggcgg cgagcgcacg
    16441 gccgtgagcg cggcaaccgt cgacgttttc ctcgagagcg cctggttcag cccgcagatc
    16501 atcgccggcc gcggccggcg ccatggcctg cacaccgatg cgagccagcg attcgagcgc
    16561 ggtgtcgatc cggcaggcca ggtcgaggcg atcgagcgtg cgacggtact gctcacgcag
    16621 atcgcaggtg gccggccggg cccgacgcag agggtcgagc gccccgagtt cctgcccgcg
    16681 cgcgcgccgg tgccgctgcg tcgcgcgcgc ctgtcgcggc tgttgggcca ggcaccggcc
    16741 gacgccgcgg tcgaggggca cctgcgtgcg ctcggcatga tcgtcgagcg caacgctgaa
    16801 ggctgggcag caacgccacc gggccatcga ttcgatatca cgatcgaagc ggacctgatc
    16861 gaggaagtgg cgcggctgca gggcctcgat gccattgccg gggcaccact gcacgcagcg
    16921 cagcggatcg ccgcgcgccc ctcgtccgag cccgatgagg cacgcgcgct cgaactgctc
    16981 gccgcgcgtg gctaccacga ggcgatcaac ttcgcctttg tcgatgccgc actgcagtca
    17041 cgcctgttcc ccggcgcgca ggctgcggcg ctggccaacc cgatcgccag tgacctcgcg
    17101 gtcatgcgcg tctcgttgtg gccgggcctg ctgcgtgcac tgcgcgaaaa ccagcggcac
    17161 cagcaggagc gtgtacgtct cgtcgagcac ggtacggtgt tcctcgtcga cggcgcagca
    17221 acgcgcgagg tcgacatgat cgcgggcgtc gcgagcggca cgcgcatgcc ggagcagtgg
    17281 ggcagtgcga aggccacggt tgacttccat gacgtgaagg gcgacatcga ggccctgctg
    17341 gccacgaccg gcgacagcgc acgcatcgaa ttcgtggcgg gcgcgggcgc gtgcctgcac
    17401 ccgggccgca gcgccgggat cctgcgcggc gggcgacgcg taggctcgct cggcgaactg
    17461 catccggaac tcgtgcgggc gctcgacttg acatatgtcc cggtgctttt cgagctcgag
    17521 ttcgcggctc tggcgaccgg catgccgcga ttccaggaag tttcgcgttt cccccaggtg
    17581 cggcgcgacc ttgccatcgt cgtcgacgaa tcgacgcctt ttagcgcgat tcgcgaacgt
    17641 gtaaccttgg cggggtcgag cctgttgcga gaccttctcg ttttcgatga ataccggggg
    17701 cccggagtag aatccggacg aaaaagcatc gctttaggct tgatttttca ggataattct
    17761 cgcacgctga ctgatcaaga tgcggaccgt atcgtggcgg ctatcgttgc cgacctggcc
    17821 ggtgcgctga atgcgaaaac aagagagtag gcatggccct gaccaaggcg gaaatggccg
    17881 aggcgctttt caatcagctg ggattgaaca agcgtgaagc gcgcgagctc gtcgatctat
    17941 tctttgagga agtacgtgcg gcgctgtcga atggcgaaca ggtgaagctt tccggttttg
    18001 gaaacttcga cctgcgcgac aagaaccagc ggccgggtcg caatccgaag acaggcgaag
    18061 aaattcccat cacggctcgc cgtgtggtga cattccgccc ggggcagaag ctcaaggcgc
    18121 gggtcgaggc gtatgctgga agcaaagaat aacgacgaac tgccggcgat cccgggcaag
    18181 cgctacttca cgatcggtga ggtgagcgag ctgtgcggtg tgaagccgca tgtgctgcgc
    18241 tactgggagc aggaattccc gcagctgaag cccgtgaagc gccgcggcaa tcgccgctac
    18301 taccagcgcc aggacgtaat cgtgatccgc cagatcagga gcctgctgta cgaacagggc
    18361 ttcacgatcg gcggtgcgcg caacaagctg accggcgagg aggcgcgcag cgataccacg
    18421 cagagccagc agatcgtgcg ccaggtgcgt atggaactcg aggacttgct ccgaatcctg
    18481 cgccgctagg ccagggtggt atcattcagt tccgcacggc gtccccggcc gtgcgcagtc
    18541 cggatcgaac aaatgtcgga gcgtggcgca gcctggtagc gcacttgcat ggggtgcaag
    18601 gggtc
//
LOCUS       DAOQVY010000043        18334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_3497_length_18334_cov_5.350840, whole genome shotgun sequence.
ACCESSION   DAOQVY010000043 DAOQVY010000000
VERSION     DAOQVY010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18334
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3497_length_18334_cov_5.350840"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PKN91_05850"
     CDS             <1..457
                     /locus_tag="PKN91_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27630.1"
                     /translation="FREGGYEAAPRSEEARSGEGFWVSLAPQRDAAGETRVLARLARA
                     GITDARIMVDTDSRRISVGLFSDREGAERRAAEVQRLGYAPDVTERLHIGTTWWVDLH
                     LSTAQELAEAEAFNRDASGQLELKPCPATGSAEPAALAGPAGEGAVESG"
     gene            462..534
                     /locus_tag="PKN91_05855"
     tRNA            462..534
                     /locus_tag="PKN91_05855"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:495..497,aa:Thr,seq:tgt)
     gene            complement(596..850)
                     /locus_tag="PKN91_05860"
     CDS             complement(596..850)
                     /locus_tag="PKN91_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27631.1"
                     /translation="MMNLLISLVSGAVGGNVGGALAKAKSMGPVWNTILGAVGGVGGG
                     QLAAAIGGLASLGQAGNVGSSAVIGALLPIVVGMLKKKAA"
     gene            complement(932..1729)
                     /locus_tag="PKN91_05865"
     CDS             complement(932..1729)
                     /locus_tag="PKN91_05865"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001389.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNS27632.1"
                     /translation="MEISAFAALVLGIVEGLTEFLPISSTGHLIITSSLLDLHGPKVL
                     AFEIIIQGAAILAVCWEYRRLLWKTTVSIARDAGAQRFALNIFLGFLPLAVLGLAFQA
                     QIEEFLFRPVPVAIALVIGGIAILWVDRRHGAQPTLDTVEAVPVRTAIVLGLWQALAL
                     IPGTSRAGATIIGGVWLGMSRRLATEYSFFLAIPTLIGATVYKLYEGRDLFIAADLVP
                     FTIGAVVAFITALLAVRGFIRFISTHSFAAFGWYRIVFGVIVLLIPW"
     gene            complement(1754..2062)
                     /locus_tag="PKN91_05870"
     CDS             complement(1754..2062)
                     /locus_tag="PKN91_05870"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNS27633.1"
                     /translation="MKHHNEQERLELERRLHTRHRARTSVYITVPGGRKRLCKAINLS
                     ATGVFIETTNLGLRKGQQVQLAFAINLGAVTKLHRRTATVAHVSRGGTGLMMDAYATR
                     "
     gene            complement(2115..3083)
                     /locus_tag="PKN91_05875"
     CDS             complement(2115..3083)
                     /locus_tag="PKN91_05875"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNS27634.1"
                     /translation="MRLVLLLALLPASALAGGELKLATWNLEWLIAPAELQALRTSCT
                     PQGVRPPIRARSVPCDVAHRLDRSRADFDALARYARLLDADVVALQEVDGADAARLVF
                     PGYRFCFTGRRHVQNTGFAIRAGIPFRCGKDHKALALGDSVRRGAELVLYPGESREFR
                     LLSVHLKSGCGNRLFTDRRRECAVAARQVPILEDWIDGEAAAGRPFAVLGDFNRNLQR
                     DQGAARSASGAQLRLWPEIDDGDPPEADLTNAADGNRFVNCAMNQTFSGYIDFVLLSR
                     TLAAALVPGSFDRVVYRTEDAVKRKLSDHCPVSVRIRPPAGAKKLD"
     gene            complement(3157..3585)
                     /locus_tag="PKN91_05880"
     CDS             complement(3157..3585)
                     /locus_tag="PKN91_05880"
                     /inference="COORDINATES: protein motif:HMM:NF028042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HNS27635.1"
                     /translation="MRVQQRGVTLIELMTVIVILAILASLAVPSYRNYLLRAQRTEAT
                     TALLRVQAAQEKFYLQNNAYTDDVDGDPPAGLGLAAVTERGFYDIAVDLAADGQSYEA
                     TATPVAGAGQADDTRCTEFSVNNAGERGAEGTGGLDLCWR"
     gene            complement(3605..7060)
                     /locus_tag="PKN91_05885"
     CDS             complement(3605..7060)
                     /locus_tag="PKN91_05885"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HNS27636.1"
                     /translation="MSFPQKPIRITLGAALCTIFVSLPAVADDTEIYMSQPESTSARP
                     NVLFILDTSGSMDREVVSTVPIYDPGKTYDSLGCSADRIYYSTGNTPPSCGTDNWFPR
                     TSLHCDAAQTALENVGGSGTWPAFGNPRARAAQFRSNKWRSIRSGETGYVECGADNNV
                     HGHNTTNTTTRRYVRNTSSGHPWGSSSSLNWNNLGSSNGYRFYTANYLNYYNGEGNPE
                     EKTRLAVVRDVAISLASSLQDVNLGLMRYNTNAQGGYVLEAIDDIATNRDQFISTLKN
                     FNDTSGDGGTPLSETLFEAANYLRGGALTYGDENRPGRSVDASRTAPGGKTYRSPIQY
                     QCQKNYIVYLTDGAPTVDTDANDSIASMIGAKCAADPIEPNHDGGWVAGSGICMDDLA
                     QWLNTTDLSDSLAGTQSASTYMIGFGKDIVTAQGDTLGYLNTIAEAGGTEHAYTAEDV
                     PTLTEALQRIFSGIQEQSSTFITPSISVNTFNRAQTSSDLYFSLFKVAAGTHWPGNLK
                     KYKLDGTTIRDSNDAAAVDASGFFASGTKSIWSPTADGNDVTQGGAASQLPDPASRKL
                     YTHLPGASSDRLAVAGNAFNTTNLTDALVGAGTDTATCGTGCENAINWARGFDVYDNN
                     GDGSTTDALRFMGDPLHGRPGVVGYGGTTSTPDEFDTVLYFPTNDGFLHAISGKTGEE
                     LWAFIPPELLGRLTDLYLNVPTTTRSYGLDGDIQVLKFDKNQDGIVDPTEGDKVWLFF
                     GMRAGGTNYYALDVTDRSDPELIWTIGSDDLPGVGQTWSTPVITRVTVGASGASNDSD
                     ERFVLIFGGGYQAATQENQPYSIDTVGNRVYMVDAESGKLLWFAGGAGTGTPNLALTK
                     MTNSIPARVTVIDTDGDRFADRMYAADMGGRVWRFDIHNGEPAASLVTGGVIAQLGVG
                     DNGDLDDSENRRFYNAPDVSLVQRRAEDPYYNIAIGSGYRGHPLDTVTQDRFYAFRDK
                     QPYARLTQTAYNAITPIVDGDLVDITADPAGSTVTTSDAGWKLVLPDQTGRDGEKVLA
                     EATTVNNVILFTTFQPLSGGAADPCYPANINRAYAVTVDTGKPALDFNDDGVLDNDDL
                     SVDLSQSGIVGEISVALIRNGVDGENTAPTEPPTVCVAGVEVLKKCVSAGGTIRTFWQ
                     RDDAN"
     gene            complement(7090..7482)
                     /locus_tag="PKN91_05890"
     CDS             complement(7090..7482)
                     /locus_tag="PKN91_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27637.1"
                     /translation="MNTSAGKTMKHHIQRPLLTVALGILAAGAAFAQTASRPGPVRFE
                     TVTMAVEVFAGSVTLPSGPNGTLVMATCHDCPLQSFTTDTATGYFVDGRPASLAEVRA
                     AATAAPRNILTVSYVVKTGVVTQISAVQ"
     gene            complement(7479..7949)
                     /locus_tag="PKN91_05895"
     CDS             complement(7479..7949)
                     /locus_tag="PKN91_05895"
                     /inference="COORDINATES: protein motif:HMM:NF025699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HNS27638.1"
                     /translation="MHASHHIAHRQQGAALVIGLILLLILTLLAVTGMNTASSELIMA
                     GNEQYRQNAFHAAEAGIEQAITVLATVPQTGTPVVVSDVAVPGSSGDEYTTSSTYMGD
                     DLNLPGFSAGKFVGFHYQIESRGISARNANSAQVQGAFVIQSAGGAGSFGSINP"
     gene            complement(7958..9013)
                     /locus_tag="PKN91_05900"
     CDS             complement(7958..9013)
                     /locus_tag="PKN91_05900"
                     /inference="COORDINATES: protein motif:HMM:NF027400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HNS27639.1"
                     /translation="MKRRETGLSLIELLVALAIGSVLIVGAVYVYSQSRTTYVVNDIV
                     ARLQEDARYAMSVIEPDVQLAGYYGFTNSPDDFKYITLGSTASVVTASRMQAADTAVA
                     GLQGGFHDCGNNFAINIVATVEGSDDSYGLGCAAQGGGAVATADTLTIRRASREDFAA
                     ATDGRLQLLVSRLSPTNQYIISDGTLPTSPALEPDTVQVRNLVVRSYYVAQDSTAPDR
                     VGLPALRVKTLADTGFIDEQVMPGVEDLQVQFGIDTGDYDNDGNIDPGLDVDGNGIPD
                     APRGIATRYVDPDALPVGFQVVSVRVWLLMRSSQFEAGFVDGRTYDYAGKSVTPNDGW
                     RRMLISRTIQLRNSRAL"
     gene            complement(9010..9492)
                     /gene="pilV"
                     /locus_tag="PKN91_05905"
     CDS             complement(9010..9492)
                     /gene="pilV"
                     /locus_tag="PKN91_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HNS27640.1"
                     /translation="MTRNFLSRQRGITMVESLVALVVLAVGLLGIASLYVTSLQTGRT
                     ALIRTQAVNLVSDMGDRIRANGRAGDAYDTDTYGGAPEAQDCVVTANCTAATLAEDDL
                     ARWIEAVETTLPRPVATVTFIPAAAAGRPDQYQINVQWQETTEQFNYQASTFLIPVQP
                     "
     gene            complement(9503..10096)
                     /locus_tag="PKN91_05910"
     CDS             complement(9503..10096)
                     /locus_tag="PKN91_05910"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HNS27641.1"
                     /translation="MKTRSTGFTLMELMIVLALAAVILAIGAPNFNEFRRNNRLTGVG
                     NDLLGAVQTARNEAIKRQVPVAVCASANPGDDDAACSDGDYTGWIVFADPDNNCERND
                     ADPDEDVIRVGHTVDAALTVTATGSCVSFGANGFRQTLAGIDTAGNILFCDDRGTAEQ
                     AGTGLSAARGIEVSATGRSRVTRDMVELGAAPWPACP"
     gene            complement(10213..10404)
                     /locus_tag="PKN91_05915"
     CDS             complement(10213..10404)
                     /locus_tag="PKN91_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27642.1"
                     /translation="MNEDNLARRIDSSGERVAVPAISVPARPTLRIVQGTAPVVRLRR
                     RKPFRLEFPDSGYRPFRVR"
     gene            complement(10459..11541)
                     /locus_tag="PKN91_05920"
     CDS             complement(10459..11541)
                     /locus_tag="PKN91_05920"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNS27643.1"
                     /translation="MSSRQYAALPDPFAMHRGGVLRGARIAWESWGTLSPAKDNAILL
                     FTGLSPSAHAAASAADPSPGWWDPILGPGLAIDTQRYFVIAVNSLGSCFGSTGPASTN
                     PATGKTYRLDFPELAVEDIANGGYEVLRSLGIERAHTVIGPSLGGMVVLAFAAQHPGV
                     ARQLVSISGSIASSPFAIALRSLQREAILADPAWQAGQYAPDAPPRNGLRLARKLGTI
                     TYRSAGEWDQRFGRERIPGAPQEANSFAHEFQVESYLEAQAEKFVAAFDANCYLYLSR
                     AMDRFELETLPTACVQRALVIGVTTDILFPIGDQRRIARALERSGAATELVEMPSIEG
                     HDAFLVDIPRFSVAIRDFLRMSSSSP"
     gene            complement(11538..12479)
                     /gene="cysK"
                     /locus_tag="PKN91_05925"
     CDS             complement(11538..12479)
                     /gene="cysK"
                     /locus_tag="PKN91_05925"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNS27644.1"
                     /translation="MIYDSILGTIGRTPIVRINRLAPQHVTLYVKCEFFNPASSVKDR
                     LAIAIIEDAERRGALKPGQTVVEATSGNTGIALAMVCAAKGYPFVATMAETFSVERRK
                     IMRMLGARVILTPAAERGQGMVRKAAELAKKHGWFLANQFENQANPAYHRQTTGAEIV
                     SDFAGRRLDFFVTGWGTGGTLTGAGEVIKLARPGTSVIACEPAAASLLGGAEFTPHKI
                     QGWTPDFIPGVLNRKIADRIVTVTDQEAIEAARALAKNEGIFCGISSGGTFAAAIKVA
                     AEAPRGSVLLAMLPDTSERYLSTPLYEGIAEGSDPEP"
     gene            12508..13257
                     /locus_tag="PKN91_05930"
     CDS             12508..13257
                     /locus_tag="PKN91_05930"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNS27645.1"
                     /translation="MSSPERLQKLLAAAGLGSRRAIEDWLRAGRVAVNGRIATLGDRA
                     TRNDAITVDGRHVNLAPREARREVLMYHKPLGEVTTRRDPQGRPTVFERLPRPAAGRW
                     IVVGRLDVNTTGLLLFTTDGELAHRLMHPSSEVEREYLVRVHGEPDDATLRRLQEGVR
                     LEDGYGRFDRVERQARTSSHDWFRVVLREGRNREVRRLWSAVGFEVSRLMRVRFGPVS
                     LPQDLGPGNARRLERDEVDALLAACTAKGEP"
     gene            13254..13790
                     /locus_tag="PKN91_05935"
     CDS             13254..13790
                     /locus_tag="PKN91_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27646.1"
                     /translation="MMKSACLAVLAAMCIAVTGEARANVIAAAADGFTIVIEVDVAVT
                     PDVAYLKFVNGPALWWDDAHTYSGKAAHMSLDPRAGGCFCEQIDGGGSISHGTVLYAD
                     PGNALRLSAALGPLQEMAVIGTLSVAFAPRTEGTHVTLTYRVFGGFMTDAVELSKLVD
                     TVMTTQLQRYAAHAGRAG"
     gene            13796..14188
                     /locus_tag="PKN91_05940"
     CDS             13796..14188
                     /locus_tag="PKN91_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27647.1"
                     /translation="MPTRRAALLALGSAVVVGAASATVRAQAPADSARATFDLELESC
                     GKALRRVARLFSSGFGRDEADRLAREIDALPSEQRGQWTFTVGFEGGTATLAVQALVD
                     DLSMVDLDFTTSPEIATRIRKAIGNLER"
     gene            14281..15954
                     /gene="hutU"
                     /locus_tag="PKN91_05945"
     CDS             14281..15954
                     /gene="hutU"
                     /locus_tag="PKN91_05945"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNS27648.1"
                     /translation="MDTFRTRDVVIRAARGNKLTARSWLTEAPLRMLMNNLDPEVAER
                     PGDLVVYGGIGRAARNWQCYDQIVAALTRLGDEETLLIQSGKPVGIFRTHADAPRVLL
                     ANSNLVARWSDWDHFRELDRKGLMMYGQMTAGSWIYIGSQGIVQGTYETFAEMGRQHY
                     GGNLAGRWILTAGLGGMGGAQPLAATMAGASMLAIECRPERIAKRLETGYLDEQAPSL
                     EAALARIDAACARREAVSVGLLGNAAQVLPELLARGVRPDAVTDQTSAHDPVNGYLPP
                     GWTLAQWDELRVSDPARVAAEARKGMACHVEAMLGFAKLGIPVFDYGNNIRQVAFDEG
                     VREAFSFPGFVPAYVRPLFCRGIGPFRWVALSGDPQDIHRTDEKVKALLPHDAALHRW
                     LDMARERIQFQGLPARICWVGLGERHRLGLAFNEMVRSGEVSAPIVIGRDHLDSGSVA
                     SPNRETEAMLDGSDAVADWPLLNALLNTASGATWVSIHHGGGVGIGYSQHAGVVIVCD
                     GTDAAARRIERVLWNDPASGVMRHADAGYALAIDCAREQGLDLPFLGGQ"
     gene            15957..17189
                     /gene="hutI"
                     /locus_tag="PKN91_05950"
     CDS             15957..17189
                     /gene="hutI"
                     /locus_tag="PKN91_05950"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNS27649.1"
                     /translation="MPPPAALWRNARVVTCTSVADAAHPQADAIVTVGGRIDWVGAET
                     ALAPERRANCSAVHDLGGRLVTPGLIDCHTHLVFAGDRAAEFAERQRGVSYEEIARRG
                     GGILATVRATRAADEQALYEAALPRFESLLREGVVALEIKSGYGLTLEDEARMLRVAR
                     TLGARHDVVVRTSYLAAHAVPPEFAGRADDYLHALASEWLPELVRRGLVDAVDVYCDR
                     GAFSAAQSRALFEVARGLGLPVRMHVGQFADVGGAQVAAEFAAVSCDHLEEISGPDVQ
                     RLAAAGTVAVLLPVAYYTLRQVRPPPVELLRAAGVPLAVATDCNPGTSPCASLLLAMN
                     MGCRLFGLHAEEALAGVTCHAAQALGLAPAQGSLAAGAGADFVVWDAAGTHELSYWTG
                     LNRCRAVIRRGALARGAL"
     gene            17195..18184
                     /gene="hutG"
                     /locus_tag="PKN91_05955"
     CDS             17195..18184
                     /gene="hutG"
                     /locus_tag="PKN91_05955"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050415 - formimidoylglutamase activity
                     [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HNS27650.1"
                     /translation="MLTEDWRGRIDAGDGDSGRRWHQVVQPLTAESPPGTALLGFACD
                     EGVSRNQGRTGASEGPRALRRALAPLAWHGAAGGALYDAGDIACVDGDLEGAQRALGG
                     RVATLLATGHFPMLLGGGHEIAWGSFQGAAAALEDSGRLSRYGIINFDAHFDLRQGPP
                     SSGTPFFQIYQSLNSKDRAFRYLCLGISRASNTPALFHIAQQCGARWLSDEALTWAGW
                     EPARRAVDAFIDELDALQLSVCLDAFPAAAAPGVSAPAGRGLAPDLGLALLAHVLARA
                     GHGTAKSKVVLAEVAELAPRLDPDGRTARLAARVVHDIVTPTPRASATSRAST"
BASE COUNT         2745 a         5951 c         6566 g         3072 t
ORIGIN      
        1 ctttcgcgag ggtggctacg aggcggcgcc ccgcagcgag gaagcgcgca gcggggaggg
       61 attctgggtg tcgcttgcgc cgcagcggga cgcggccggc gaaacgcgcg tgctcgcgcg
      121 cctcgcgcgc gcgggcatca ccgatgcgcg catcatggtc gatacggatt cgcggcgtat
      181 ctcggtgggc ctcttcagcg atcgcgaagg cgccgagcgt cgggcggccg aagtgcagcg
      241 gctgggctat gcgccggatg tgaccgagcg gctgcacatc ggcacgactt ggtgggtcga
      301 cctgcacctg agcacggcgc aggaactggc cgaggccgag gcgttcaacc gcgacgcctc
      361 gggccagctc gagctgaaac cctgtcccgc gacgggctcc gcagagccgg cggcgctggc
      421 gggaccggcg ggtgagggcg cggtagaatc cggctgacgg cgccggctta gctcagttgg
      481 tagagcacct gatttgtaat cagggggtcg cgggttcgat ccctgcagcc ggcagattcg
      541 catggcgggc gaagcggcca tgccgctcca cccgcacacg cggcggctcc cggcgtcagg
      601 ccgctttctt cttcagcatc ccgacgacga tcggcagcaa agcgccgatc acggccgagc
      661 tgccgacatt gccggcctgg ccgagtgacg cgaggccgcc gatcgcggcg gccagctggc
      721 cgccaccgac cccgccgacg gcgccgagga tcgtgttcca gaccgggccc atgctttttg
      781 ccttcgccag tgcgccaccg acgttgccgc cgactgcgcc gctcacgagg ctgatgagca
      841 gattcatcat gccagcatcc atgtggtccc ccctgtgttt ggttatcgga tgggtgcgac
      901 tccactatac gccgcttccg tgcgggcgcg cctaccaggg aatgagcagc acgatgacgc
      961 cgaacacgat ccggtaccag ccgaatgccg cgaagctgtg ggtgctgatg aagcggatga
     1021 agccgcgcac ggcgagcaga gccgtgatga atgcgacgac cgcgccgatc gtgaacggca
     1081 ccaggtccgc ggcgatgaac aggtcgcggc cctcgtagag tttgtacacg gtcgcaccga
     1141 tcagcgtcgg tatcgcgagg aagaacgagt attcggtggc gaggcgccgc gacatgccga
     1201 gccacacgcc gccgatgatc gtcgcgccgg cgcgcgacgt gcccgggatc agcgcgagcg
     1261 cctgccagag cccgagcacg atggcggtgc gcaccgggac cgcttcgacc gtgtcgaggg
     1321 tcggctgcgc gccgtggcgc ctgtcgaccc acaggatcgc gatgccgccg atgaccagcg
     1381 cgatggcgac cggtaccggg cggaagagga attcttcgat ctgcgcctgg aaggcgagcc
     1441 ccagcacggc gagcggcagg aagccgagga agatgttgag cgcgaaacgc tgtgcgccgg
     1501 cgtcacgggc gatcgacacc gtggtcttcc acaggagcct gcggtactcc cagcacaccg
     1561 cgaggatggc cgcgccctgg atgatgatct cgaaggcgag gaccttcgga ccatgcaggt
     1621 cgagcagact gctggtgatg atcaggtgcc ccgtgctcga gatcggcagg aactcggtga
     1681 gtccctcgac aatgccgagc acgagcgcgg cgaatgcgct gatttccatg gggacggcga
     1741 cggcctgcca cgctcagcgc gtggcgtagg catccatcat caggccggtg ccaccgcgcg
     1801 acacatgggc gaccgtggcg gtgcgccggt gcagcttggt cacggcgccg agattgatcg
     1861 cgaaggcgag ctggacctgc tggcccttgc gcagcccgag attggtggtc tcgatgaaga
     1921 cgccggtggc ggacaggttg atcgccttgc acaggcgttt gcgaccgccg ggaacggtga
     1981 tgtagacgga agtgcgtgcc cggtggcgtg tgtgcaggcg gcgttccaac tcgagacgct
     2041 cttgttcgtt gtggtgtttc atggcccccg cctgattatg ccttccgccg ccagtttgcc
     2101 aaacgcgagt gaacttaatc gagtttcttt gcgcccgccg gcggccggat ccgtaccgag
     2161 accggacaat ggtcggatag tttgcgcttg acggcatcct cggtgcgata gacgacgcgg
     2221 tcgaaggagc ccggcacgag cgcggcggcg agggtccgcg acagcagcac gaagtcgata
     2281 tagccggaaa acgtctggtt catcgcgcag ttcacgaagc ggttgccgtc cgccgcgttg
     2341 gtgaggtccg cctccggggg gtcgccgtcg tcgatctcgg gccacagcct gagctgcgcc
     2401 ccggacgcgg agcgcgccgc gccctggtcg cgctgcagat tgcggttgaa gtccccgagc
     2461 accgcgaacg gcctgcctgc cgcggcctcc ccgtcgatcc agtcttcgag gatcggcacc
     2521 tgcctcgccg cgacggcgca ttccctgcgc ctgtccgtga acaggcggtt gccgcaaccg
     2581 gacttcaggt gcacggacag cagccggaac tcgcgcgact cgccggggta gagcaccagt
     2641 tcggcgcccc ggcggacgct gtcgccgagc gccagcgcct tgtggtcctt gccgcagcgg
     2701 aaaggaatcc cggcgcggat cgcaaagccg gtgttctgca cgtggcgcct tcccgtgaag
     2761 cagaatcgat agccgggaaa cacgagtcgc gccgcgtcag ccccgtcgac ttcctgcagc
     2821 gcgacgacat ccgcatcgag gagccgcgca tagcgcgcca gggcgtcgaa atcggcccgg
     2881 ctgcgatcga gacgatgggc gacgtcgcag ggtaccgagc gggccctgat cggcggccgc
     2941 acgccctgtg gtgtgcagga tgtgcgcagc gcctgcagtt cagcgggcgc gatcagccat
     3001 tcgaggttcc aggtggcgag cttcagctcg ccgcccgcga gggcgctggc gggcagcaga
     3061 gcgaggagca ggacgaggcg catcgcggca ttgtagccgc ctgctgctgc ggcctcgccc
     3121 gatctccggc cggcgctgcg cgccccgcgc agttgctcag cgccagcaca ggtcgaggcc
     3181 gcccgtgccc tcggcgccgc gctcgcccgc attgttcacc gagaactcgg tgcaacgcgt
     3241 gtcatcggcc tggcccgcgc ccgcgaccgg cgtggccgtg gcttcatagc tctggccgtc
     3301 ggcggccagg tccacggcga tatcgtagaa accccgctcg gtgacggccg cgaggcccag
     3361 ccccgcgggc gggtcaccgt cgacgtcgtc ggtgtaggcg ttgttctgca ggtagaactt
     3421 ctcctgcgcc gcctgcacgc gcagcaacgc cgtcgtcgcc tcggtgcgct gtgcgcgcag
     3481 caggtagttg cggtagctcg gcacggcgag cgacgccagg atcgcgagga tgacgatgac
     3541 cgtcatcagc tcgatcaggg tgacgccgcg ttgttgcact cgcatggctc gcctccggga
     3601 cagattagtt ggcatcgtcg cgctgccaga aggtgcggat ggtgccgcca gcgctgacgc
     3661 acttcttcag cacctcgaca cccgcgacgc acacggtcgg cggctcggtc ggcgcggtgt
     3721 tctcgccgtc cacgccgttg cggatcagcg cgacgctgat ctcgccgacg ataccgctct
     3781 ggctgaggtc gaccgacagg tcgtcgttgt cgagtacgcc gtcgtcgttg aagtcgagcg
     3841 ccggcttgcc cgtgtcgacg gtcaccgcat acgcgcggtt gatgttggcc gggtagcagg
     3901 gatcggccgc cccgccggac agcggctgga aggtcgtgaa gaggatcacg ttgttcactg
     3961 tggtcgcttc cgccagtacc ttttcgccgt cacggcctgt ctggtcgggc agcaccagct
     4021 tccagccggc atcgcttgtg gtcacggtcg accccgcggg atcggccgtg atgtccacca
     4081 ggtcgccgtc gacgataggc gtgatggcat tgtaggccgt ctgcgtcagg cgtgcgtacg
     4141 gctgtttgtc gcggaatgca tagaaacgat cctgggtgac ggtatcgagc gggtgcccgc
     4201 ggtagccgga gccgatcgcg atgttgtagt acgggtcctc tgcgcggcgc tgcaccagcg
     4261 acacgtcggg ggcgttatag aagcggcggt tctcgctgtc gtcgaggtcg ccgttgtcgc
     4321 ccacgccgag ctgcgcgatc acgccgccgg tcaccagcga ggccgcgggc tcgccgttgt
     4381 ggatatcgaa gcgccacaca cggccgccca tgtcggccgc gtacatgcgg tccgcgaagc
     4441 ggtcgccgtc ggtatcgatc accgtgacac gggccggaat cgagttcgtc atcttggtca
     4501 gcgcgaggtt cggcgtgccg gtgcccgcgc cgcccgcgaa ccacagcagc ttgccgctct
     4561 cggcatcgac catatagacg cgattgccga cggtatcgat gctgtagggc tggttctcct
     4621 gggttgcggc ctggtagccg ccgccgaaga tcagcacgaa gcgctcatcg ctgtcgttgc
     4681 tggcacccga ggcgccgacc gtgacgcgcg tgatcaccgg cgtcgaccag gtctggccga
     4741 cacctggcag gtcatcgctg ccgatcgtcc agatgagctc cgggtcactg cggtccgtga
     4801 cgtcgagcgc gtagtagttg gtgccaccgg cgcgcatgcc gaagaacagc cacaccttgt
     4861 caccctcggt ggggtcgacg atgccgtcct ggttcttgtc gaacttcagc acctggatgt
     4921 cgccgtcgag gccgtagctg cgggtcgtgg tcggcacgtt caggtacagg tcggtgagcc
     4981 ggccgagcag ttcgggcgga atgaaggccc acagctcctc gccggtcttg ccgctgatcg
     5041 cgtgcaggaa gccgtcgttg gtcggaaaat agagcaccgt gtcgaactcg tcgggtgtcg
     5101 aggtcgtgcc gccatagccg acgacgccgg ggcggccgtg cagcgggtcg cccatgaagc
     5161 gcaaggcatc ggtcgtgctg ccgtcaccgt tgttgtcgta cacgtcgaac ccgcgcgccc
     5221 agttgatggc gttctcgcag ccggtgccgc aggtcgcagt gtcggtgccg gcaccgacca
     5281 gcgcgtcggt caggttggtg gtgttgaacg cattgccggc caccgccagc cggtcggagc
     5341 tcgcgcccgg cagatgcgtg tagagcttgc gcgaagcggg atcgggcagc tggctcgcgg
     5401 cgccgccctg ggtcacgtca ttgccgtcgg ccgtcggcga ccagatgctc ttcgtgccgc
     5461 tggcgaagaa gccgctcgca tcgacggccg ccgcgtcgtt cgagtcgcgg atcgtcgtgc
     5521 cgtcgagctt gtatttcttc aggttgcccg gccagtgcgt gccggcggcc accttaaaca
     5581 gcgagaagta caggtcgctg ctcgtctgcg cgcggttgaa ggtgttgacc gagatcgacg
     5641 gggtgatgaa ggtcgagctc tgctcctgaa tgccgctgaa gatgcgctgc agcgcctcgg
     5701 tcagtgtcgg cacgtcctcg gcggtgtagg cgtgttcggt gccgccggcc tcggcgatcg
     5761 tgttgaggta gccgagcgta tcgccctggg ccgtcacgat gtccttgccg aaaccgatca
     5821 tgtaggtgga ggcgctctgc gtgcctgcca ggctgtccga caggtcggtg gtgttgagcc
     5881 actgcgccag atcgtccatg cagatgccgc tgccggccac ccaaccgccg tcgtggttcg
     5941 gctcgatcgg atccgcggcg cacttcgcgc cgatcatcga ggcgatgctg tcgttcgcgt
     6001 cggtgtcgac ggtcggggca ccgtcggtca ggtagacgat gtagttcttc tggcattggt
     6061 actggatggg cgaccggtag gtcttgccgc cgggcgcggt gcgcgacgca tcgacgctgc
     6121 ggccaggccg gttctcgtcg ccataggtca gcgcgccgcc acgcaggtag ttggccgcct
     6181 cgaacagggt ttcggacagc ggtgtgccgc cgtcgcccga ggtgtcgttg aagttcttca
     6241 gcgtggagat gaactggtcg cggttcgtgg cgatgtcgtc gatcgcctcg agtacatagc
     6301 cgccctgcgc attggtgttg tagcgcatca ggcccagatt cacgtcctgc agcgaggacg
     6361 ccaggctgat cgcgacgtcg cgtaccaccg cgaggcgcgt cttctcttcc ggattgcctt
     6421 caccgttgta gtagttcagg taattcgccg tgtagaagcg atagccgttg ctgctgccga
     6481 ggttgttcca gttcaggctc gaggaactgc cccaggggtg gccggagctg gtgttgcgca
     6541 cgtacctgcg cgtcgtcgta ttggtggtgt tgtggccgtg cacgttgttg tcggcgccgc
     6601 actcgacgta gccggtttca ccgctcctga tgctgcgcca cttgttgctg cgaaactgcg
     6661 ccgcgcgcgc gcgggggttg ccgaaggcag gccaggtgcc gctgccgccg acgttctcca
     6721 gcgccgtctg cgcggcgtcg cagtgcagcg aggtacgcgg gaaccagttg tcggtgccgc
     6781 agctgggcgg tgtgttgccg gtgctgtagt agatccggtc agcgctgcag ccgaggctgt
     6841 cataggtctt gcccggatcg tagatcggca ccgtcgagac gacctcgcgg tccatgctgc
     6901 ccgacgtgtc gaggatgaac agcacgttcg gccgcgcgga ggtggactcg ggctggctca
     6961 tgtagatctc cgtgtcgtct gccacggcgg gcaggctcac gaagatcgtg cacagggcgg
     7021 cgcccagggt gatgcgaatc ggcttttggg ggaacgacat ggctgggtcc tcgggatacg
     7081 gcgcggggtt cactgcaccg cggagatctg tgtcacgacg cccgtcttga cgacgtagga
     7141 gacggtcagg atgttgcggg gcgcagcggt ggccgcggcg cgcacttcgg cgagcgacgc
     7201 gggcctgcca tcgacgaaat acccggtggc ggtgtccgtc gtgaacgact gcagcgggca
     7261 gtcgtggcag gtcgccatca cgagcgtacc gttcggcccg ctcggcaggg tgaccgagcc
     7321 cgcgaagacc tcgaccgcca tcgtgacggt ttcgaagcgg acgggaccgg ggcgcgatgc
     7381 cgtctgcgca aatgcggcgc ctgccgccag gatgccgagc gcgacggtga gcagtgggcg
     7441 ctggatgtgg tgcttcatgg tcttgcctgc agaggtgttc aagggttgat gctgccgaag
     7501 gaaccggcgc cgccggcact ctggatcacg aacgcgccct gcacctgcgc cgaattcgca
     7561 ttgcgtgccg agatgccacg gctctcgatc tggtagtgga agccgacgaa cttgccggcg
     7621 ctgaagcccg gcaggttcag gtcatcgccc atgtaggtgc tcgaggtcgt gtactcgtcg
     7681 ccgctcgatc cgggcaccgc cacgtcgctg acgacgaccg gggtgccggt ctgcggcacg
     7741 gtggcgagca cggtgatggc ctgctcgatg cccgcttcgg cggcatggaa ggcgttctgc
     7801 cggtactgct cgttgccggc catgatcagc tccgagctcg cggtgttcat gccggtgacg
     7861 gcgagcaggg tcaggatcag cagcaggatc aggccgatga cgagtgcggc gccctgttgc
     7921 cggtgtgcga tatggtggct tgcgtgcatg gcgtggctca cagggcgcgc gaattgcgca
     7981 gctggatggt tcgcgagatg agcatgcggc gccagccgtc gttcggcgtg acgctcttgc
     8041 ccgcgtagtc gtaggtgcgg ccgtcgacga acccggcctc gaactgcgag ctgcgcatca
     8101 gcagccagac acgcaccgag accacctgga aaccgaccgg cagcgcatcg gggtcgacgt
     8161 agcgcgtggc gatgccgcgc ggcgcatcgg gaataccgtt gccgtcgacg tcgaggccgg
     8221 gatcgatatt gccgtcgttg tcgtagtcgc ccgtgtcgat gccgaactgc acctgcaggt
     8281 cctcgacgcc gggcatgacc tgctcgtcga tgaagcccgt gtcggccagc gtcttgacgc
     8341 gcagcgcggg caggccgacg cggtccggcg cggtggagtc ctgcgcgacg tagtagctgc
     8401 gcacgaccag gttgcgcacc tgtaccgtgt cgggctcgag ggcgggcgaa gtcggcaggg
     8461 tgccgtccga gatgatgtat tgattggtcg ggctcaggcg ggacacgagc agctgcagac
     8521 gcccgtcggt ggccgcggcg aagtcctcgc gcgaggcccg gcggatcgtc agggtgtcgg
     8581 cggtggcgac ggcaccgccg ccctgcgccg cgcagccgag accgtagctg tcgtcgctgc
     8641 cctcgaccgt tgcgacgatg ttgatcgcga agttgttgcc gcagtcgtgg aagcctccct
     8701 gcagacccgc gacggcagtg tccgccgcct gcatgcgcga ggccgtgacg accgaggcgg
     8761 tcgagccaag ggtgatgtac ttgaaatcgt ccggcgagtt cgtgaagccg tagtagccgg
     8821 cgagctgcac gtcgggttcg atcaccgaca tcgcatagcg cgcatcctcc tgcaggcgcg
     8881 cgacgatgtc gttcacgacg taggtggtgc ggctctgcga gtacacgtac acggcgccga
     8941 cgatcagcac cgaaccgatc gcgagcgcga ccagcagttc gatcaggctg agacccgtct
     9001 cgcggcgttt catggctgca ccgggatcag gaaggtgctg gcctgataat tgaactgctc
     9061 ggtcgtttcc tgccattgca cgttgatctg gtactggtcg gggcggccgg ccgccgcggc
     9121 cggaatgaag gtcaccgtgg cgacgggacg cggcagcgtg gtctccaccg cctcgatcca
     9181 gcgggcaagg tcgtcctcgg cgagcgtcgc agccgtgcag ttggcggtca cgacgcagtc
     9241 ctgggcctcg ggcgcgccgc cgtaggtgtc ggtgtcgtag gcatcgcccg cgcgaccgtt
     9301 ggcgcgaatg cgatcgccca tgtcgctcac caggttcacg gcctgcgtgc ggatgagcgc
     9361 ggtgcggccg gtctgcaggc tggtgacata caggctcgcg atgccgagca gcccgaccgc
     9421 gagcacgacc agcgcgacga gggattcgac catcgtgatg ccgcgctgcc gggaaaggaa
     9481 gttgcgtgtc atcgcggtca cctcacgggc aagccggcca cggggcggct ccaagttcga
     9541 ccatgtcgcg cgtgacgcgc gagcgtccgg tggcgctcac ttcgatgccg cgcgccgctg
     9601 acaggccggt accggcctgc tcggcggtgc cgcggtcgtc gcagaagagg atgttgccgg
     9661 ccgtatcgat gccggccagc gtctggcgaa agccgttcgc accgaacgac acgcagctgc
     9721 ccgtcgcggt gacggtgagc gcggcatcga ccgtatggcc gacgcgaatg acgtcttcgt
     9781 ccgggtcggc gtcattgcgc tcgcagttgt tgtccggatc ggcgaagacg atccagcccg
     9841 tgtaatcgcc gtccgagcat gcggcatcgt cgtcgccggg attggcggat gcgcagaccg
     9901 ccacgggcac ctggcgcttg atcgcctcgt tgcgcgcggt ctgcacggca ccaagcaggt
     9961 cgttgccgac gccggtgagc cggttgttgc gccggaactc gttgaagttc ggcgcgccga
    10021 tcgcgaggat gaccgcggcc agcgccagca cgatcatcag ctccatcagg gtaaaacccg
    10081 tgcttcgcgt tttcatgggc cggcagtgtg ccggcaacgc aagggggccg ccgagcctcc
    10141 cgactagcgg cgcgcgcgcg gggacgaaag gcgcggcgcc gtgaactgca tcacacggcg
    10201 tcgcgacata attcagcgca cgcggaacgg gcggtagccc gagtcgggaa attcgaggcg
    10261 aaacggcttg cgacgacgca ggcgcaccac cggcgcagtg ccctgcacga tccgcagtgt
    10321 cggccgcgcc ggtacgctga tggccggtac ggcgactcgt tcgccgctgc tgtcaatccg
    10381 tcgcgcgaga ttgtcttcgt tcatgtcctg ctcctcgttg tgtcatgcac attgtggtgc
    10441 gcggatatga cacgcgtatt aaggagagct cgaagacatg cgaaggaagt cacggatcgc
    10501 gacggaaaag cgcgggatgt cgacgaggaa cgcatcgtgg ccctcgatgg agggcatttc
    10561 gacgagctcg gtcgcggcac cgctgcgctc gagcgccctc gcgatccggc gctgatcgcc
    10621 gatcgggaag aggatgtcgg tggtgacgcc gatgacgagc gcgcgctgca cgcaggcagt
    10681 cggcaaggtt tcgagctcga agcggtccat cgcgcgtgac aggtacagat agcagttcgc
    10741 atcgaaggca gcgacgaact tctcggcctg ggcctcgagg taactctcca cctggaattc
    10801 gtgcgcgaag ctgttcgcct cttgcggggc accggggatg cgttcgcgcc cgaagcgctg
    10861 gtcccactca ccggcggatc ggtaagtgat ggtgccgagc ttgcgcgcca gcctgagccc
    10921 gttgcgcggc ggggcatcgg gcgcgtactg gcccgcctgc cacgcgggat ccgcgaggat
    10981 cgcctcgcgt tgcaggctgc gcagggcaat ggcgaatggc gaggatgcga tgctgccgct
    11041 gatgctcacg agctggcggg cgacgcccgg gtgctgggcg gcgaaggcga gcacgaccat
    11101 gccgccgagc gacgggccga tgaccgtgtg cgcgcgctcg atcccgaggc tgcgcagcac
    11161 ttcgtagccg ccgttggcga tgtcttcgac cgccagttcc gggaagtcga gccgataggt
    11221 cttgccggtg gccgggttgg tcgatgccgg gccggtggaa ccgaagcaac tgccgagcga
    11281 attcaccgca atcacgaaat agcgctgcgt gtcgatcgcc agtccggggc cgaggatcgg
    11341 gtcccaccag cccggcgagg gatcggcagc cgaggccgcg gcatgtgccg acggcgacag
    11401 cccggtgaac agcaggatgg cattgtcctt cgccgggctc agcgtgcccc agctttccca
    11461 ggcgatgcgc gcgccgcgca acaccccacc ccggtgcatc gcgaacggat cgggcagcgc
    11521 cgcgtactgg cgcgagctca tggttccggg tcgctgccct cggcgatgcc ctcatacagg
    11581 ggcgtcgaca ggtatcgctc ggacgtgtcg ggcagcatcg cgagcagcac gctgccgcgc
    11641 ggcgcctcgg ctgcgacctt gattgccgcg gcgaaggtgc cgccgctcga gatgccgcag
    11701 aagatgcctt cgttcttcgc gagtgcgcgc gcggcctcga tcgcttcctg gtcggtgacg
    11761 gtgacgatgc gatcggcgat cttgcggttc agtacgccgg gaatgaagtc cggggtccag
    11821 ccctggatct tgtgcggcgt gaattcggcg cccccgagca gcgaggcggc cgcaggctcg
    11881 caggcgatga cgctggtgcc gggccgcgcg agcttgatga cctcgcccgc gccggtgagc
    11941 gtgccgccgg taccccagcc cgtgacgaag aagtcgagcc ttcggcccgc gaagtcgctg
    12001 acgatctcgg cacccgtggt ctggcgatga taggcggggt ttgcctggtt ctcgaactgg
    12061 ttggcgagaa accagccatg cttcttcgcg agctcggcgg ccttgcgcac catgccctgg
    12121 ccacgctcgg cggcgggggt gaggatgacc ctcgccccga gcatgcgcat gatcttgcgc
    12181 cgctcgaccg agaaggtctc cgccatggtc gcgacgaacg ggtagccctt cgccgcgcag
    12241 accatcgcga gggcgatgcc ggtattgccc gaagtcgcct cgacgacagt ctgtcccggt
    12301 ttcaatgccc cgcgccgctc ggcgtcctcg atgatcgcga tcgcgagccg atccttgacc
    12361 gaagaggcgg gattgaagaa ctcgcacttc acgtacaagg tcacgtgctg gggtgcgagg
    12421 cggttgatgc gaacgatggg cgtgcgcccg atcgtgccga ggatgctgtc gtaaatcatg
    12481 atggcactct tgattatgat ggtggctgtg tcgtcccctg aacgcctcca gaaactgctc
    12541 gcggccgcag gcctcgggtc gcgccgcgcc atcgaggact ggctccgtgc cggtcgcgtc
    12601 gccgtaaacg gtcggatcgc gacgctcggt gaccgcgcga cccgtaatga tgccatcacc
    12661 gtcgacgggc ggcacgtgaa tctcgccccg cgggaggccc gccgcgaggt gctcatgtat
    12721 cacaagccgc tcggcgaggt gaccacgcgg cgcgacccgc aggggcggcc caccgtgttc
    12781 gaacgcctgc cacggcccgc ggcgggacgc tggatcgtgg tcgggcggct cgacgtcaat
    12841 accaccggcc tgctgctgtt caccacggat ggagaactcg cacaccgcct gatgcaccct
    12901 tcgagcgagg tggagcgcga atacctcgtg cgggtccatg gcgagccgga cgacgcgacg
    12961 ctgcggcgcc tgcaggaggg cgtgcgactt gaggacgggt acggccggtt cgaccgcgtc
    13021 gagcgccagg cccgcaccag cagccacgac tggttccgcg tggtgttgcg cgaaggccgc
    13081 aatcgggaag tgcggcgtct gtggagcgcg gtgggtttcg aggtgagccg gctgatgcgt
    13141 gtgcgcttcg gtcctgtaag cttgccgcag gacctgggcc ccggcaacgc gcgccggctc
    13201 gaacgcgacg aggtcgatgc gctgctggcg gcctgtaccg caaagggaga accatgatga
    13261 agagtgcgtg cctcgccgta ctggccgcca tgtgcatcgc cgtcacgggc gaagcccggg
    13321 cgaatgtcat cgccgccgcc gccgatggct tcacgatcgt catcgaggtg gatgtggcgg
    13381 tgacgccgga tgtcgcctac ctgaagttcg tcaacgggcc cgcgctgtgg tgggacgacg
    13441 cgcacaccta tagtggcaag gcggcgcaca tgagcctcga tccgcgcgcg ggcggctgct
    13501 tctgcgaaca gatcgacggc ggcggctcga tcagccacgg cacggtgctc tatgccgacc
    13561 ccggcaatgc gctgcgcctc tcggcggcac tcggcccgct gcaggaaatg gccgtgatcg
    13621 gcacgctgtc ggtggcgttc gcgccgcgta ccgagggcac gcatgtcaca ctgacttacc
    13681 gtgtattcgg cgggttcatg acggatgccg tcgagctctc gaagctcgtc gacacggtga
    13741 tgaccacgca gctgcagcgc tacgccgcgc acgccggccg cgccggttga cggccatgcc
    13801 gacgcgtcgc gccgcgctgc ttgcgctggg ctccgccgtg gtggtcggcg cggcgtccgc
    13861 caccgtgcgc gcgcaggccc ccgcggattc cgcgcgcgcc accttcgacc tcgaactcga
    13921 aagctgcggc aaggcactgc ggcgcgtggc gcggctgttc agcagcggct tcggccgcga
    13981 cgaggccgac cggctcgcgc gcgagatcga cgccttgccg tccgagcagc gtggccaatg
    14041 gaccttcacg gtcggcttcg aaggcggcac cgccacgctc gccgtccagg cgctggtcga
    14101 cgatctgtcg atggtcgatc tcgacttcac cacctcgccg gagatcgcga cgcgcattcg
    14161 caaggcgatc ggcaacctgg aacgctgacg ggggtcgggt ggcatccggt gtcgtcaatc
    14221 ggccgacggc tcggcttcga attgtgtttt cttcatcggc cccgggtaca gtttttgacc
    14281 atggacacat ttcgcactcg tgatgtagtg attcgcgcag ctcgcggcaa caagctcacc
    14341 gcgcgcagct ggctcaccga ggcgccgctg cgcatgctga tgaacaacct tgatcccgag
    14401 gttgccgagc gccccggaga cctcgtggtc tacggtggca tcgggcgcgc ggcgcgcaac
    14461 tggcagtgct acgaccagat cgtcgcggca ctcacgcgcc tcggtgacga agaaacgctg
    14521 ctgatccagt ccggcaagcc ggtcggcatc tttcgcacgc atgccgatgc gccgcgggtg
    14581 ttgctcgcca actcgaacct cgtcgcccgc tggtccgact gggatcactt ccgcgaactc
    14641 gaccgcaagg gcctgatgat gtacggccag atgacggccg gctcgtggat ctacatcggc
    14701 agccagggca tcgtgcaggg cacctacgag accttcgccg agatggggcg ccagcattac
    14761 ggcggcaacc tagcggggcg ctggatcctg accgcgggtc tcggcggcat gggcggtgcg
    14821 cagccgctgg ccgcgacgat ggcgggtgca tcgatgctcg cgatcgaatg ccggcccgag
    14881 cgcatcgcca agcggctcga gacgggctac ctcgacgagc aggcgccgtc gctcgaggcg
    14941 gcgctcgcgc gcatcgacgc cgcatgtgcg cggcgcgagg ccgtgtcggt cggcttgctg
    15001 ggcaacgcgg cgcaggtcct gcccgaattg ctcgcgcgtg gcgtgcggcc ggatgcggtg
    15061 accgaccaga cctcggcgca cgatccggtc aacggttacc tgccgcccgg ctggacactg
    15121 gcgcagtggg acgagctgcg cgtgagcgac ccggcgcggg tggccgcgga ggcacgcaag
    15181 ggcatggcgt gccatgtcga ggccatgctc ggattcgcga agctcggcat cccggtcttc
    15241 gactacggca acaacatccg ccaggtcgcc ttcgatgaag gtgtgcgcga ggcattttcc
    15301 ttcccgggct tcgtgccggc ctatgttcgg ccgctgttct gccgcggcat cggtccgttc
    15361 cgctgggtcg cgctgtcggg cgatccgcag gacatccatc gcaccgatga aaaggtgaag
    15421 gcgctgctgc cccatgacgc cgcgctgcac cgctggctcg acatggcgcg cgagcgcatc
    15481 cagttccagg gtctgcccgc gcgcatctgc tgggtcgggc tgggcgagcg gcatcgcctg
    15541 ggcctcgcct tcaacgaaat ggtccgcagc ggcgaagtgt cggcgccgat cgtgatcggc
    15601 cgcgatcacc tcgattcggg atcggtcgcg agcccgaacc gcgagaccga agcgatgctc
    15661 gacggttcgg acgcggtcgc cgactggccc ttgctgaatg cgctgctcaa caccgcgagc
    15721 ggcgcgacct gggtgtcgat ccaccatggc ggtggcgtcg gcatcggcta ttcgcagcac
    15781 gccggagtcg tgatcgtgtg cgacggcact gatgcggccg cgcgccgcat cgaacgcgtg
    15841 ctgtggaacg acccggcgag cggcgtgatg cgccatgccg atgccggcta tgcccttgcg
    15901 atcgattgcg cgcgagagca ggggctcgac ctgccgttcc tcggcgggca atgacgatgc
    15961 cgccgcccgc ggcactgtgg cgcaacgcga gggtcgtgac ctgcacgtcc gtggccgacg
    16021 ccgcgcatcc gcaggccgac gcgatcgtca cggtgggtgg gcgcatcgac tgggtcggcg
    16081 cggagacagc acttgccccg gagcgccgcg cgaactgttc cgccgtgcac gacctcggcg
    16141 ggcggctggt cacgcccggc ctgatcgact gccacaccca cctcgtgttc gccggggatc
    16201 gcgcggcgga gtttgccgag cgccagcgcg gcgtgtcgta cgaggagatc gcgcgtcgcg
    16261 gcggcggcat tctcgccacg gtacgcgcaa cacgcgcggc ggacgagcag gcgctgtacg
    16321 aggcggcgct gccgcgcttc gaatcactgc tgcgcgaggg cgtcgtggcg ctcgagatca
    16381 agtccggcta cggcctcacg ctcgaggacg aagcgcgcat gctgcgcgtg gcgcgcacgc
    16441 tcggagcgag gcacgacgtc gtcgttcgca ccagctatct cgccgcgcac gcggtgccgc
    16501 cggagttcgc gggccgcgcc gacgattacc tgcacgcgct tgcgagcgag tggctgccgg
    16561 aactcgtgcg ccgcgggctc gtcgacgcgg tcgacgtcta ctgcgatcgg ggcgcgttca
    16621 gcgccgcgca gtcacgcgcg ctgttcgagg tcgcgcgcgg actgggcctg ccggtgcgca
    16681 tgcacgtggg ccagttcgcc gatgtcggcg gtgcgcaggt ggccgcggag ttcgcggccg
    16741 tgtcctgcga ccatctcgag gaaatctccg ggccggatgt gcagcgcctg gcggcggcgg
    16801 ggacggtggc cgtgttgctc cctgtggcgt attacacgct gcgccaggtc cgcccgccgc
    16861 cggtcgagtt gctgcgtgcg gcgggcgtgc cgctcgcggt cgcgaccgac tgcaatccgg
    16921 gcacctcgcc ctgcgcctcg ctgctgctcg cgatgaacat ggggtgtcgc ctgttcggcc
    16981 tgcatgccga ggaagcgctc gcgggggtga cctgtcatgc ggcccaggcg ctgggacttg
    17041 cgccggcgca aggcagcctc gccgccggcg ccggcgcgga tttcgtcgtc tgggacgcag
    17101 cgggcacgca cgagctgagt tactggaccg ggctcaatcg ctgccgcgcg gtgattcgcc
    17161 gcggcgcact cgcgcggggt gcactgtgaa ggccatgctg accgaagact ggcggggtcg
    17221 gatcgacgcc ggcgatggcg acagcgggcg ccgctggcac caggtcgtgc agccgctcac
    17281 ggcggaatcg ccccctggaa ctgccctgct tggtttcgcc tgcgacgagg gggtcagccg
    17341 caaccagggc cgcaccggtg ccagcgaggg cccgcgcgcg ctgcgccgcg cgctcgcgcc
    17401 gctcgcgtgg catggggccg caggaggtgc gctctacgat gccggggaca ttgcctgcgt
    17461 ggatggcgac ctcgagggtg cgcagcgagc gctcgggggg cgtgttgcga cgctgctggc
    17521 cacggggcat ttcccgatgc tgctcggtgg gggccacgag atcgcctggg gctcgttcca
    17581 gggggccgcc gccgcgctcg aggatagcgg ccgattgtcg cgctacggca tcatcaattt
    17641 cgatgcgcac ttcgacctga ggcaggggcc accgagctca ggtacgccat tttttcagat
    17701 ctatcaatct cttaattcga aagaccgtgc atttcgatac ctctgcctcg gaatctcgag
    17761 agcatcgaat acgccagcat tgtttcatat cgcgcaacaa tgcggtgccc gctggctgtc
    17821 cgatgaagcc ctcacctggg ccggctggga gccggcccgt cgcgcggtcg acgccttcat
    17881 cgacgaactc gacgccctgc agctgagcgt gtgcctggac gcgtttcctg cggctgcggc
    17941 acccggggtc agcgcacctg ccgggcgcgg gctcgcgccg gacctgggcc tggcgctgct
    18001 cgcgcatgtg cttgcgcgtg ccggtcatgg cacggcgaag tcgaaagtgg tactcgccga
    18061 agtggcggag ctcgcgccga ggctcgaccc cgacggacgc acggcgcgtc tcgcggcgcg
    18121 cgtcgtgcat gacatcgtga cccccacgcc ccgcgcatcc gccacgtccc gcgcatccac
    18181 atagctaagg tgtgcattgc agtgggcggt ccaagcaaga cgcgcgccaa ctgcattgac
    18241 aggtcgccgg ctggacacga gaatacgcgg ctcgtttttg cggaggggta cccaagcggc
    18301 caacgggagc agactgtaaa tctgccggct tacg
//
LOCUS       DAOQVY010000044        17196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_3800_length_17196_cov_5.481886, whole genome shotgun sequence.
ACCESSION   DAOQVY010000044 DAOQVY010000000
VERSION     DAOQVY010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17196
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3800_length_17196_cov_5.481886"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="PKN91_05960"
     CDS             <1..880
                     /locus_tag="PKN91_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNS27651.1"
                     /translation="LFADVVGSTRLFEELGDVRARDTIATCIDVMRTATEEHNGTVIK
                     TMGDEVMATFPSADEALSAAAQMQQQISVHAHLKNDGHPVAIRIGCHFGPVVLENRDV
                     FGATVHTANRMTSQAKAGQIVTTATTVELLAPEWRASVRQIDVATLKGQGSEVTLFEV
                     LWQTEDVTSMVPAIALAGRESRRSVRLRLRTFDQEVVLDDHRPQASIGRAEDNDLVVK
                     GNLISRLHARIETSRNKFVLIDQSTNGTFVQGSSGEEAFVRRDSMQLKGEGMIGLGRT
                     PEHGSPQTIRYVCEES"
     gene            complement(891..2666)
                     /locus_tag="PKN91_05965"
     CDS             complement(891..2666)
                     /locus_tag="PKN91_05965"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNS27652.1"
                     /translation="MATAPRALTPLSQLAKPVADWVADVASLTKPDHIHWCDGSAGEI
                     RRLRNELVASGELHALEEKSFPGCDLYRSHPGDVARVEQVTFICTTKRDDAGPNNLWM
                     SPADAHARMRELFSGCMKGRTLYVVPYCMGPIDSPLARCGVEITDSAYVVLNMAIMTR
                     MGRPALERIASGESFVRGLHSIGELDPNRRFIMHYPEELAIESFGSGYGGNALLGKKC
                     HALRIASWQARDEGWLAEHMLIVGLENPAGEQHYIAAAFPSACGKTNLAMLVPPDSLK
                     GWKVTTVGDDIAWLHPGPDGRLWAINPEAGYFGVVPGTNRTTNRNAYDLIRHNTLFTN
                     VAVTADNQPWWEGLENGTPVIDWQGRPYDPGNGPAAHPNSRFTVSATQNPSYSPHAED
                     PQGVPISAIVFGGRRRSVAPLVYEARDFNHGVLVGAGMASEQTAAAEGKVGLVRRDPM
                     AMKPFCGYNFADYWAHWIKVGAGLANPPRIYHVNWFRRDANGKFLWPGYGDNLRVLAW
                     MLDRCAGRGGATETPIGNLPRASDLDLAGLDIAPDALAALLTVDHDDWRKEAAEIRAY
                     LDEFGERLPAALARELDALEQRLGA"
     gene            2814..4742
                     /locus_tag="PKN91_05970"
     CDS             2814..4742
                     /locus_tag="PKN91_05970"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNS27653.1"
                     /translation="MTDVDEVFSAGGPLARALPDYSPRRSQRRMATRVAEAIAACRSL
                     LVEAGTGTGKTFAYLVPALLSGRRVIISTGTRTLQDQLFGKDLPLVAAAIGRPARVAL
                     LKGRANYLCRHRLFAATAQRALAGFEGGSLAHIERWAAVTKTGDLAEVPELADAGSTR
                     AAITSTRENCLGQRCAEWARCHVVAARRAANDADIVIVNHHLLLADLALKEDGFGDLL
                     GTADAVILDEAHQLPDLVTQFFGVEASSRQVEHLLGDARAELTRVGGDAAAGEALAAV
                     DGALGQVRAALPSRAGNGATLRMAWDEASVSLEAALDTLADSLGTLHSALAAHGDEAA
                     LAHCAARAARVAETLERISHAREDEAARTIERDGRGFTLSMLPFDVAPRFRGIVDARP
                     CAWIFTSATLSVGEDFAHFASRLGMADAETLQIDSPFDFERQGLLYLPPAMPDPSAPD
                     YLAAVLGEALPLIEAARGGAFLLFTSHRALARAAQMIAARAEGPLPYPLLVQGTAPRE
                     QLLRDFRAQGNAVLLGTASFWEGVDVKGEALRLVVIEKLPFASPEDPLVKARIAHLNA
                     VGGNPFRDYQLPEAALALKQGVGRLIRSEDDFGVVAICDPRLVSRGYGRVLRASLPAL
                     TVTRERDAASRFLARRAA"
     gene            4739..5449
                     /gene="tsaB"
                     /locus_tag="PKN91_05975"
     CDS             4739..5449
                     /gene="tsaB"
                     /locus_tag="PKN91_05975"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNS27654.1"
                     /translation="MTAHGTPRAMKILALDTATEACSVALWLDGQVVLDAIELAHGHA
                     QRILPMIDALLAAHALRLTDLDGIAVGCGPGGFTGLRLAVSIAQGLALGADLPAVGVS
                     NLAAVAQAMFDRDPAVEKVLVCNDARMNEVYTGLYRRGGDGLAVPAGAERVLSPDAVA
                     APEGAAVGAGRGFRAYPQLAQRLRASLGYIDDAVLPDAGAIVRLAAARLAAGEGVAAD
                     ALQPVYLRDNVAVAKPLV"
     gene            5535..6230
                     /gene="phoB"
                     /locus_tag="PKN91_05980"
     CDS             5535..6230
                     /gene="phoB"
                     /locus_tag="PKN91_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HNS27655.1"
                     /translation="MSAREILIVEDERPIREMIAYGLRRAGFEVREAEDCREAKQQLA
                     DRQPDLLLLDWMLPDMSGLELAKHLKRDKDTRELPIIMLTARAEEADKVSGLEGGADD
                     YMTKPFSPRELVARINAVLRRMEPGGGDDRIEFGGLVLDRAGHRVSAGEDTVPLGPTE
                     HRMLEFFMTHPERVYSRAQLLDRVWGGNVYVEERTIDVHIRRLRKALEPFGLDHLLQT
                     VRGAGYRFSVRAH"
     gene            6239..7537
                     /gene="phoR"
                     /locus_tag="PKN91_05985"
     CDS             6239..7537
                     /gene="phoR"
                     /locus_tag="PKN91_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HNS27656.1"
                     /translation="MRTVLALAGVLALGGVIGLITGRVALWLLAALLAYTALQLWKLH
                     RLAEWLGKRNFSDPPSLGGVWDEIVTQVMRLNRRKRYHKQRFLAMLHEVRDSTEALPD
                     GVVLLNARSEIVGLNRMASEMLGLHGRKDIGIRIDNLLRQPEFLGYLAGSDFTSPKVL
                     QTSTAPETWLSLHVLPYGEGQRVLLIRNVTREVRLDAMRKDFVANASHELRSPLTVIT
                     GYLEALAGDEQVSEQYAAPIAEMRRQADRMAAIIGDLLVLSRLEAKEGEVDGEAVDIV
                     AMLALLKKDALARSRRPANVTLTIESHSRLLGDEIEPHSAFSNLLDNAIKYTPADGSV
                     AMRWWADEAGAHFSVSDTGPGIPAEAIPRLTERFYRVDAGRSRASGGSGLGLAIVKHV
                     LQRHGGRLEIASVEGRGSTFTCHFPQRRHAPPGAGSNTTH"
     gene            7562..8302
                     /gene="phoU"
                     /locus_tag="PKN91_05990"
     CDS             7562..8302
                     /gene="phoU"
                     /locus_tag="PKN91_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNS27657.1"
                     /translation="MEATDLSQHISSRFNEDLEAVRANLLAMGGLVEEQLRKGLAALT
                     DADSTLAGEVVRDDAKVNAMEVAIDRECLRILATRSPAAGDLRLVIAVIKAITDLERI
                     GDEARKIANISLHLATLEQPSDRYREVRHLGRLVEHMLRSALDAFARMDVDAALHIAQ
                     LDRQVDEEYESIQRQCVTFMMEDPRQIRRMLDLSWAARSLERVGDHATNIGEYVVYLV
                     RGQDIRHRNIAEVETHPGGSAKGGGTAR"
     gene            8302..8820
                     /locus_tag="PKN91_05995"
     CDS             8302..8820
                     /locus_tag="PKN91_05995"
                     /inference="COORDINATES: protein motif:HMM:NF014639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HNS27658.1"
                     /translation="MENRRLGNALGAAAAFAMLAFALYAEKVLGLPPCPLCMFQRAAV
                     LALGIVFAVAALHDPGKAGGRVYAVLIVLAAGAAVAVAARHVYVQSLPPGTVPACGAS
                     LDFMLDTFPLLEVVRRVMTGGGECARIDWQFLGLSMPAWVLVASAVLGIFGAAVNAGP
                     RSALRRTGFARL"
     gene            complement(8792..9919)
                     /gene="dapE"
                     /locus_tag="PKN91_06000"
     CDS             complement(8792..9919)
                     /gene="dapE"
                     /locus_tag="PKN91_06000"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HNS27659.1"
                     /translation="MTDTLALTLNLIARNSVTPTDSGCQQVMMQRLAAAGFEVEPLRY
                     GVVDNFWAKRGTGGPVFCFAGHTDVVPTGPLEEWQSDPFQPTIRDGVLYGRGAADMKS
                     GLAAMVTATEAFVRERPRYQGSIAFLITSDEEGPSVDGTRRVVEELKARGEHIDWCVV
                     GEPSSEQELGDTIKIGRRGSLSGRLTVHGVQGHIAYPQFAENPVHTFAPALAELTSRV
                     WDEGNEHFQPTTFQISNFNAGTGAPNVIPGELKARFNLRYGPVQTLAGLRETVEGILR
                     KHGVRFTLEWYVSGEPFYTPAAELSHAVIEAVRAETGKAPRLSTGGGTSDGRFIAPMG
                     AQVVELGVVNATIHKVNECVRVGEIDALHRIYLRVLRTLFA"
     gene            10050..11360
                     /locus_tag="PKN91_06005"
     CDS             10050..11360
                     /locus_tag="PKN91_06005"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HNS27660.1"
                     /translation="MNKTAFIAVSALLAMPALGQQVPSTTIDELRRDLEALTQRLERV
                     EAENAYLKDNARANRKEIAEADVALARLPDIEKAGKAADWAGRIKLKGDFRYRHENID
                     DATAAKDRTRHRVRVRLGVEAKVNDAVKAVVQVATAGDDRDPRSTNQTLGEGFTRKGL
                     GLDLAYADWRPVQGLNVQLGKIPYAWQRVGSYFWDGDLTWEGGAVRFARGAFFGSAMG
                     AWLSESSSGADANVFGGQIGVKTRLGDAIGFTGAVGYFDVSAVQNEITLTSATPACAA
                     NKVFFGGAQGNRTDVVAGCRRLRDDYNMIEALAQLDFKVGDLPLAVFGNYIRNQEAAD
                     NDTGYAFGATLGKAGDPRTWELSYVYQDMEQNAQFAQFVDSDFGAGTTFSKGSVVKAA
                     WAPAKNWTVNGTYFFNERVPPGAAAVSETWRDYDRLQLDLNYRF"
     gene            11428..12429
                     /locus_tag="PKN91_06010"
     CDS             11428..12429
                     /locus_tag="PKN91_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNS27661.1"
                     /translation="MKLTSLFTLIAAGIAALAFAPGDASAQSVVKIDGSSTVFPISEA
                     VAEEFQIARRGKVRVTVGISGTGGGFKKFCRGETDISDASRPISTGEMAACAKAGVQY
                     IELPIAYDALTVAVNPKNTWVDSITVEELKKIWEPAAQGKITTWKQVNPKWPAQPLKL
                     FGPGADSGTFDYFTEAVVGKSKSSRGDFTASEDDNVLVQGIESDVNALAYFGYAYYAP
                     NSSKIKALRIVNSKGQAVGPSPQGVLDGSYEPLSRPIFIYVNKASAQRPEVRDFVEFY
                     LQKGAPLVEEVKYVPLAKPAYDKALANFRAGKAGTAFGGHNEVGLTVDELLAREAKF"
     gene            12445..13368
                     /gene="pstC"
                     /locus_tag="PKN91_06015"
     CDS             12445..13368
                     /gene="pstC"
                     /locus_tag="PKN91_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNS27662.1"
                     /translation="MTAHSSSPASLRRSIRRRRVRDRLIEALLLAAGLVAVFTTLAIV
                     WILVVEALPFFEHVPLRDFLTDTMWTPLFAEPRYGILPLVAGTLVTTFVALLVAIPAG
                     TIIAIYLSEFASHRLRETVKPALEMLGAVPTIVYGYFALVMVTPFLQKFFPGLPGFNM
                     LSAGLVIGSMIVPYVASVSEDAMRAVPNYMREGSYATGATRFQTATRVVVPAALSGIA
                     AAYILGISRAVGETMVVAVAAGLQPNLTWNPAESAATITAFIVQVSLGDLPHDSIGYQ
                     SIFAAGLVLLLITLVFNIAGFALTRRFREAY"
     gene            13368..14303
                     /gene="pstA"
                     /locus_tag="PKN91_06020"
     CDS             13368..14303
                     /gene="pstA"
                     /locus_tag="PKN91_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNS27663.1"
                     /translation="MAVVTEDPSIHALRADVSRRKLFDLAFVIIGLVVLFASLLVLLV
                     LFLDLVMQGAPRLNWDFFTSYASRKPENAGILAAWIGTTLIMVVTACVAMPIGVAAAI
                     YLEEYAPKNWFTSIIEINVTNLAGVPSIIYGLLALGLFVYKFSLGQSIAAAGLTLALL
                     ILPIVIVATREAVRAVPRTIREAAYALGATRWEVTAHHVLPNSMGGVLTGMIIGLSRA
                     IGETAPIITIGALSFIAFLPSPPLTAEFPFLSFAWLRDSFTAMPIQMFNWVSRPDPGF
                     RVNAAAAGAILLGMTLLMNGVAIWLRYRFRKRINW"
     gene            14456..15190
                     /gene="pstB"
                     /locus_tag="PKN91_06025"
     CDS             14456..15190
                     /gene="pstB"
                     /locus_tag="PKN91_06025"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNS27664.1"
                     /translation="MSFYYGSVQALKSLDMEISDKQVTALIGPSGCGKSTFLRCFNRM
                     HDLTPMTRYEGSIRLFPDDIDLIAKDVDPIEVRMRIGMVFQKPNPFPKSIFENVAYGL
                     RVRGMRSKAQIEEQVEKALREAAIWDEVKDRLHTSGLSLSGGQQQRVCIARALATNPE
                     ILLFDEPTSALDPIATARIEELITGLRGRVTVLIVTHNMQQAARVSDYTAFMYLGELV
                     EFDVTSKLFTNPAKKATEDYITGRYG"
     gene            15202..15894
                     /gene="phoU"
                     /locus_tag="PKN91_06030"
     CDS             15202..15894
                     /gene="phoU"
                     /locus_tag="PKN91_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNS27665.1"
                     /translation="MEPAATLDVAMSSLRLKVLEMGGLVVDQVTTAVRALLEYDRDAA
                     ELVLARERQVNAYDTAIEAAGFELLALRAPVATDLRGVLAIAHVVTDLERVGDEAKRI
                     ARFTLAAMARPGFDPSVAVHRHLRHMAHQSARMLRIAIDALDKGDATAAHQVARMDRE
                     IDREFDAALRELLTVAMEGPTQLRGTIDTVFALKSLERIGDHAKNVAERVVFIVSGED
                     VRYMRANHDGRG"
     gene            complement(15918..16751)
                     /gene="dapD"
                     /locus_tag="PKN91_06035"
     CDS             complement(15918..16751)
                     /gene="dapD"
                     /locus_tag="PKN91_06035"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNS27666.1"
                     /translation="MTDHTALRTIVDDAFEHRAELSPGKVPADVAAAIERAIGLLDSG
                     EARVAEHDGRHWVVNEWLKKAVLLYFRTHDNTVVDAGYARFYDKVPLKFATDDEASLR
                     ASGVRVVPPAIVRKGAYVAPNVVLMPSYVNIGAHVGAGTMVDTWATVGSCAQIGRNVH
                     LSGGVGIGGVLEPLQAAPTIIEDDCFIGARSEVVEGVIVERGSVISMGVFLGQSTRIY
                     DRETGIVLHGRVPAGSVVVPGTLPSTDGTHSLACAVIVKKVDAKTRAKTSLNDLLRTD
                     D"
     gene            complement(16789..>17196)
                     /locus_tag="PKN91_06040"
     CDS             complement(16789..>17196)
                     /locus_tag="PKN91_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNS27667.1"
                     /translation="DIAAAQRFIERVGALGAHFALDDFGTGFSSLAYLKSLPVSALKI
                     DGSFVRDLLTDERSEGVIRAVLQIASQLGLDTVAEYVETTAIADRLRSLGVTYGQGYA
                     FGAPVPLDTVLAGLHADVDAGKQAAPGQVAVSG"
BASE COUNT         2701 a         5771 c         5910 g         2814 t
ORIGIN      
        1 actgtttgcc gatgtcgtcg gctcgacgcg cctgttcgag gagctggggg atgtgcgcgc
       61 gcgcgatacg atcgcgacct gcatcgacgt gatgcgcacg gccaccgaag agcacaacgg
      121 caccgtcatc aagacgatgg gcgatgaggt catggcgacc ttcccctcgg ccgacgaggc
      181 gctgagcgcc gcggcgcaga tgcagcagca gatctcggtg cacgcccacc tgaagaacga
      241 cggccacccg gtcgcgatcc gcatcggctg ccattttggt ccggtagtgc tcgagaaccg
      301 cgatgtgttc ggcgcgaccg tgcataccgc caatcgcatg accagccagg ccaaggccgg
      361 ccagatcgtc accaccgcga cgaccgtcga gctgctggcg cccgaatggc gtgcctcggt
      421 gcgccagatc gatgtcgcca cgctcaaggg gcagggcagc gaggtcacac tgttcgaggt
      481 gctgtggcag accgaggacg tgaccagcat ggtgccggcg attgcgcttg ccggccgcga
      541 gtcgcgccgc tcggtgcgac tgcgcctgcg taccttcgat caggaggtcg tgctcgacga
      601 ccatcggccc caggcgtcga tcggtcgagc cgaggacaac gacctcgtcg tcaagggcaa
      661 cctgatctcg cgcctgcacg cgcgcatcga gaccagccgc aacaagttcg tgctgatcga
      721 ccagagcacc aacggcacct tcgtgcaggg ctcgagcggc gaggaagcct tcgtgcgccg
      781 cgacagcatg cagctgaagg gtgagggcat gatcggcctc ggcaggacgc cggagcacgg
      841 atcgccgcag accatccgct acgtctgcga ggaaagctga gggtctggag ctaagctccg
      901 agtctctgct ccagcgcgtc gagctcacgc gccagtgccg ccggcaggcg ctccccgaac
      961 tcgtccaggt aggcgcgaat ttccgccgct tccttgcgcc agtcgtcgtg gtcgaccgtg
     1021 agcagcgccg cgagcgcatc gggcgcgata tcgaggcccg cgaggtcgag gtcgctcgca
     1081 cgcggcaggt tgccgatcgg cgtctcggtg gcgccgccgc gtcccgcaca gcgatcgagc
     1141 atccaggcga gcacgcgcag gttgtcgccg tagcccggcc acagaaactt gccgttggca
     1201 tcgcgccgga accagttgac gtggtagatg cgcggcggat tcgcgagccc ggcaccgacc
     1261 ttgatccagt gcgcccagta gtcggcgaag ttgtagccgc agaagggctt catcgccatc
     1321 gggtcgcggc gcacgaggcc gaccttgcct tcggccgccg ccgtctgctc cgaggccatg
     1381 cccgcgccga cgagtacgcc atggttgaag tcgcgcgcct cgtagacgag tggcgcgacg
     1441 ctgcggcggc gcccgccgaa cacgatcgcg gagatcggca cgccctgggg gtcttccgca
     1501 tgcggtgaat agctcgggtt ctgggtggcc gagaccgtga agcgtgaatt cggatgcgcg
     1561 gccggtccgt tgcccggatc gtaggggcga ccctgccagt cgatgaccgg cgtgccgttc
     1621 tcgaggcctt cccaccacgg ctggttgtcg gcggtgaccg cgacattggt gaacagggtg
     1681 ttatggcgga tcaagtcgta ggcgttgcgg ttggtcgtcc ggttcgtgcc gggcacgacg
     1741 ccgaagtagc ccgcctcggg attgatcgcc cacaggcgcc cgtcaggccc gggatggagc
     1801 cacgcgatgt cgtcgcccac ggtcgtgacc ttccagccct tgagcgagtc gggcggaacc
     1861 agcatcgcga gattggtctt gccgcatgcc gacgggaagg cggcggcgat gtaatgctgt
     1921 tcgccggccg ggttttcgag gccgacgatc agcatgtgct cggcgagcca gccctcgtcg
     1981 cgcgcctgcc agctcgcgat gcgcagcgca tggcacttct tgccgagcag ggcgttgccg
     2041 ccgtagccgg agccgaagct ttcgatcgcg agctcctccg ggtagtgcat gatgaagcgg
     2101 cggttcgggt cgagctcgcc gatcgaatgc aggccgcgca cgaaggattc cccggacgcg
     2161 atgcgctcga gggccgggcg gcccatgcgc gtcatgatcg ccatgttgag cacgacgtac
     2221 gcgctgtcgg tgatctcgac gccgcagcgt gcgagcggcg aatcgatcgg acccatgcaa
     2281 tagggcacga catagagcgt gcggcccttc atgcagccgc tgaacagctc gcgcatgcgg
     2341 gcgtgggcgt cggcggggga catccacaga ttgttcgggc ctgcgtcgtc ccgtttcgtc
     2401 gtgcagatga aggtgacttg ctcgacccgc gccacgtcgc ccgggtgcga gcggtacagg
     2461 tcgcagcccg ggaaactctt ctcttcgagg gcatggagct cgccggaggc gaccagttcg
     2521 ttgcgcagcc ggcggatttc acccgccgag ccgtcacacc agtggatgtg gtccggtttc
     2581 gtgaggctgg cgacatccgc cacccagtcg gcaaccggtt tcgcgagttg cgaaagcggg
     2641 gtgagtgcgc gcggcgctgt ggccatgctt tctcctcgtt cggtgagccg caaagtatag
     2701 cggccgacgc tgcattgcgg gcggccggcc tcgatgatgc accgcaatat gtcgaatcgc
     2761 gcgggggaaa tgcacctcga cagcagcgat gcgcatcgct agcgtgccgg gccatgacgg
     2821 atgtcgatga ggttttcagc gccggcggac cgctggcgcg cgcgttgccc gattattcgc
     2881 cacgccgttc gcagcggcgc atggcgacac gtgtcgccga ggccatcgcg gcctgccgtt
     2941 cgctgctggt ggaggccggt accgggaccg gcaagacctt tgcctatctc gtgccggcgc
     3001 tgctttcggg ccggcgcgtg atcatctcga ccggcacgcg caccctgcag gatcagctgt
     3061 tcggcaagga cctgccattg gtcgccgccg cgatcggccg ccccgcgcgc gtggcgctgc
     3121 tcaaaggacg cgcgaactac ctgtgccgcc atcgcctgtt cgcggccacc gcgcagcgcg
     3181 cgctggccgg tttcgagggc gggagcctcg cgcacatcga gcgctgggcg gcggtgacga
     3241 agacgggcga cctggcggaa gtccccgagc tcgccgatgc gggcagcacg cgggcggcca
     3301 tcacctcgac gcgcgagaac tgcctcggcc agcgttgtgc ggagtgggcc cgttgccacg
     3361 tcgtggcagc gcgccgcgcc gcaaacgacg ccgacatcgt catcgtcaat catcacctgt
     3421 tgctcgcgga cctcgcgttg aaggaggacg gcttcggcga cctgctcggc accgcggatg
     3481 ccgtgatcct cgacgaggcg caccagctgc cggacctggt cacgcagttc ttcggcgtcg
     3541 aggcgtcgag ccgccaggtt gaacacctgc tcggcgacgc ccgcgcggag ctcacgcgcg
     3601 tcggcggcga tgcggcagcg ggcgaggcgc ttgccgcggt ggacggggcg ctcgggcagg
     3661 tgcgtgcagc gctaccttca cgcgccggca acggtgcgac cctgcgcatg gcatgggacg
     3721 aggcctcggt gtcgctcgag gctgcgctcg acacgctggc cgactcgctc gggaccctgc
     3781 acagcgcgct cgccgcccat ggcgacgagg ccgcgctcgc gcactgcgcg gcgcgcgcgg
     3841 cgagagtcgc cgaaaccctc gagcgcatat cgcacgcgcg cgaggacgaa gcggcacgca
     3901 cgatcgagcg cgacggccgc ggcttcaccc tgtccatgct gccgttcgac gtcgcgccac
     3961 gctttcgcgg catcgtcgac gcgcgtccct gcgcgtggat cttcacgtcc gccacgctgt
     4021 ccgtgggcga ggacttcgcc catttcgcct cgcgcctcgg catggcggat gccgagacgc
     4081 tgcagatcga cagtccgttc gatttcgaac gccagggcct gctctacctg ccgccggcga
     4141 tgccggatcc atccgccccg gactatctcg cggcggtgct cggtgaagcg ctgccgctga
     4201 tcgaggcggc gcgcggcggg gccttcctgc tcttcacgag ccatcgcgcg ctggcccgcg
     4261 ccgcgcagat gattgccgcg cgcgccgagg ggccgttgcc atacccgctc ctcgtgcagg
     4321 gcacggcgcc gcgcgagcag ctgctgcgcg acttccgcgc gcagggcaat gcggtgctgc
     4381 tcggcaccgc gagcttctgg gagggcgtgg acgtcaaggg cgaggcgttg cggctcgtgg
     4441 tcatcgagaa gctgccgttc gcctcgccgg aggatccgct cgtcaaggca cgcattgccc
     4501 acctgaacgc cgtcggcggc aatccgttcc gcgactacca gctgcctgag gcggcgctcg
     4561 cgctgaagca gggcgtcggg cggctgatcc gcagcgagga cgacttcggt gtggtcgcga
     4621 tctgcgatcc gcggctcgtc tcgcgcggct acgggcgtgt gctgcgcgcc agcctgccgg
     4681 cgctcacggt cacccgcgag cgcgacgcgg ccagccgctt cctcgcgcgg cgcgcggcat
     4741 gacggctcac gggacaccgc gcgccatgaa gatcctcgcg ctcgatacgg cgaccgaggc
     4801 gtgctccgtg gccttgtggc tcgacgggca ggtggtgctg gacgccatcg aactcgcgca
     4861 cggtcatgcg cagcgcatcc tgccgatgat cgacgcgctg ctcgcggcgc acgccttgcg
     4921 gctcaccgat ctcgatggca ttgccgtcgg ctgcggcccg ggcggcttca ccggattgcg
     4981 actcgcggtc agcattgcgc aagggctggc cctgggcgcg gacttgcccg cggtgggtgt
     5041 gtcgaacctc gctgccgtgg cgcaggcgat gttcgatcgc gatcccgcgg tcgagaaggt
     5101 gctggtctgc aacgatgcgc gcatgaacga ggtgtacacg gggctctacc ggcgcggtgg
     5161 cgacgggctc gccgtgcccg cgggcgctga acgcgtgctg tcgcccgatg ccgtcgccgc
     5221 tccggaggga gcggccgtcg gcgcagggcg gggttttcgg gcctacccgc aactcgcgca
     5281 gcgcctgcgc gcgagtctcg gctacatcga tgatgccgtg ctgccggatg ccggcgcgat
     5341 cgtccggctc gcggcggcgc gcctggcggc gggcgagggc gtagcggcgg acgcgctgca
     5401 gcccgtttac ctgcgtgaca acgtcgccgt cgccaagcca ttggtttgaa aagaacttgc
     5461 cggattcaca gttctgcaat aatcgccggc tctaatactc tcgcgccgcg ccgggcgcgc
     5521 gatgtggcag ggacatgtcc gccagagaaa tactgatcgt cgaggacgag cggccgattc
     5581 gcgagatgat cgcctacgga ttgcggcgcg cgggtttcga ggtgcgcgaa gcagaggatt
     5641 gccgtgaggc gaagcagcaa ctcgccgacc ggcagcccga cctgctgctg ctcgactgga
     5701 tgctgccgga catgagcggc ctcgaactgg cgaagcacct gaagcgcgac aaggacacgc
     5761 gcgaactgcc gatcatcatg ctcacggcgc gcgccgagga ggccgacaag gtgagtggcc
     5821 tcgagggtgg tgccgacgat tacatgacca agcctttttc gccgcgcgag ctcgtggcgc
     5881 gcatcaacgc ggtgctccgc cgcatggagc cgggcggtgg cgacgaccgc atcgagttcg
     5941 gcgggctcgt gctcgatcgt gcgggtcacc gcgtctcggc gggcgaggac accgtgccgc
     6001 tgggtcccac cgagcaccgc atgctcgagt tcttcatgac tcatccggaa cgcgtgtact
     6061 cccgggccca gctgctcgac cgtgtctggg gcggcaacgt ctacgtcgag gagcgcacga
     6121 tcgacgtgca catccgccgc ctgcgcaagg cgctcgagcc tttcggcctc gatcacctgc
     6181 tgcagacggt gcgcggcgcg ggctaccggt tctcggtgcg cgcccattga agccacgggt
     6241 gcgcacggtg ctcgcactcg cgggcgtgct ggcgctcgga ggcgtcatcg gcctcatcac
     6301 cggtcgggtt gcgttgtggc tgctggccgc actgctcgcc tacactgccc tgcagctgtg
     6361 gaagctccac cgcctcgccg agtggctcgg caagcgcaat ttctccgacc cgccgtcact
     6421 cggcggggtg tgggacgaga tcgtcacgca agtgatgcgc ctcaaccggc gcaagcgcta
     6481 ccacaagcag cgcttcctcg cgatgttgca cgaggtgcgc gactcgaccg aagcgctgcc
     6541 ggatggcgtc gtgctgttga atgcgcgcag cgagatcgtc gggctcaatc gcatggcatc
     6601 ggagatgctc ggcctgcatg gccgcaagga catcgggata cgcatcgaca acctgctgcg
     6661 ccagcccgag ttcctcgggt atctcgccgg cagcgacttc acttcgccga aggtcctgca
     6721 gacgagcacg gccccggaga cctggctgtc gctgcatgtg ttgccttacg gcgagggcca
     6781 gcgtgtcctg ctgatccgca atgtcacgcg cgaggtgcgc ctcgacgcga tgcgcaagga
     6841 tttcgtcgcc aatgcctcgc acgagctgcg ttcgccgctc accgtcatca ccggctacct
     6901 cgaggcgctc gcgggcgacg agcaggtgag cgaacagtac gcggcgccga tcgccgaaat
     6961 gcggcgccag gcggatcgca tggccgcgat catcggcgac ctgctggtgc tgtcgcgcct
     7021 cgaggcgaag gagggcgagg tcgacggcga ggcggtcgat atcgtggcga tgctggcgct
     7081 gctgaagaag gatgcgctgg ctcgttcccg gcgtcccgcg aacgtgacgc tcacgatcga
     7141 gtcgcacagc cgcctgctgg gtgacgagat cgaaccgcat tccgccttct cgaacctgct
     7201 cgacaacgcc atcaagtaca ccccggccga tggcagcgtc gcgatgcgct ggtgggccga
     7261 cgaggcgggc gcgcatttct cggtgagcga cacgggtccg ggcattcccg cggaggcgat
     7321 cccgcgcctc accgaacgct tctaccgggt cgatgccggg cgctccaggg cgtccggcgg
     7381 ctccggcctc gggctcgcga tcgtcaagca cgtgctgcag cgtcacggtg gccgactcga
     7441 aatcgcgagc gtcgaggggc ggggcagcac cttcacctgc cacttcccgc agcggcgcca
     7501 tgcgccaccg ggcgcaggca gcaacacgac gcactgaccc tcggatatca ttgggcaagc
     7561 catggaagcc accgatctct cccagcacat ttccagccgc ttcaacgaag atctcgaggc
     7621 ggtgcgggcc aacctgctgg caatgggcgg gctggtcgag gagcagttgc gcaagggcct
     7681 cgcggcgctc actgacgccg acagtaccct cgccggcgag gtcgtacgcg acgacgccaa
     7741 ggtcaacgcg atggaagtcg cgatcgatcg tgagtgcctg cgcatcctcg ccacgcgctc
     7801 gccggccgcg ggagacctgc gcctggtgat cgccgtcatc aaggcgatca ccgacctcga
     7861 acgcatcggc gacgaggcac gcaagatcgc caatatttcg ctgcacctcg caacgctaga
     7921 gcagccctcc gatcgttacc gcgaggtgcg ccatctcggc aggctggtgg agcacatgct
     7981 gcgcagcgcc ctcgatgcat ttgcgcgcat ggatgtcgat gcggcgctgc atatcgcgca
     8041 gctcgaccgt caggtcgacg aggaatacga atcgatccag cgccagtgcg tgaccttcat
     8101 gatggaggac ccgcgccaga tccggcgcat gctcgacctg agctgggcgg cccgctcgct
     8161 cgagcgggtc ggcgatcacg cgaccaacat cggcgaatac gtggtgtacc tggtgcgcgg
     8221 ccaggacatc cggcaccgga acatcgccga ggtggaaacg cacccgggcg gctccgccaa
     8281 gggcggcggg accgcgcggt gatggagaat cgccggctgg gcaatgcgct gggtgccgcc
     8341 gcagcgttcg cgatgctcgc cttcgccctc tatgccgaga aggtgctcgg cctgccgcca
     8401 tgcccgctgt gcatgttcca gcgcgcggcg gtcctcgcgc tcggcatcgt attcgccgtg
     8461 gcggcgctgc acgatccggg caaggctggc gggcgcgtct acgcagtact catcgtgctc
     8521 gccgcgggcg cggccgtggc ggtggccgcg cggcacgtgt acgtgcagag cctgccgccg
     8581 ggcacggtgc cggcctgcgg cgcctcgctc gacttcatgc tcgacacctt cccgctgctc
     8641 gaggtcgtgc gccgcgtgat gaccggaggc ggggagtgcg cccggatcga ctggcagttc
     8701 ctcggcctgt cgatgccggc ctgggtgctg gtggcgtcgg ccgtgctcgg catcttcggc
     8761 gccgccgtga acgccggccc gcgctcggcg ctcaggcgaa cagggttcgc aagactctga
     8821 ggtagatgcg atgcagcgcg tcgatctctc cgacgcgcac gcattcgttg accttgtgga
     8881 tcgtggcgtt cacgacgccg agttccacca cctgggcgcc catcggcgcg atgaagcgac
     8941 cgtccgaggt gccgccgccg gtcgacagcc tgggtgcctt gccggtttcc gcacgcaccg
     9001 cctcgatcac cgcgtgcgac agttcggcgg ccggcgtgta gaacggttcg cccgacacgt
     9061 accactcgag cgtgaagcgc acgccgtgtt tgcgcaggat gccctcgacg gtctcacgca
     9121 ggccggcgag cgtctgcacg gggccatagc gcaggttgaa gcgcgccttc agttcgccgg
     9181 ggatcacgtt cggcgcgccg gtgccggcat tgaagttcga gatctggaac gtggtcggct
     9241 ggaagtgctc gttgccctcg tcccagacgc ggctcgtgag ttccgcgagt gcgggggcga
     9301 acgtgtgtac cggattttcg gcgaactgcg ggtaggcgat gtgcccctgc acgccgtgca
     9361 cggtcagtcg tcccgacagt gagccgcgcc gaccgatctt gatcgtgtcg ccgagttcct
     9421 gctcgctcga gggttcgccc acaacgcacc agtcgatatg ctcgccgcgc gccttgagct
     9481 cttcgacgac gcgccgggta ccgtccacag acgggccttc ctcgtcactc gtgatcagga
     9541 aggcgatcga gccctggtac cgcggccgct cgcgcacgaa ggcctcggtg gcggtcacca
     9601 tggcggccag gccgcttttc atgtcggcgg caccgcgacc gtagagcact ccgtcccgta
     9661 tggtcggctg gaacggatcg gactgccatt cctcgagcgg tccggtgggc accacgtcgg
     9721 tgtgaccggc gaagcagaac accggccccc cggtgccgcg cttcgcccag aagttgtcta
     9781 cgacgccgta gcgcagcggt tcgacctcga agccggcggc ggcgaggcgc tgcatcatga
     9841 cctgctggca gccgctatcc gtcggtgtga cggaattgcg ggcgatcagg ttgagggtca
     9901 gggcgagggt atcggtcatg gctggcagcg ccttatacgg ggcagcccgc ggcgccgcaa
     9961 gtcctccgtc atcggatcgt cgcggcagcg tcatgcgtct gacatgtgcg gttcgtataa
    10021 cgccatccgt cacatcaacg gagacccacg tgaacaaaac cgccttcatt gccgtcagcg
    10081 cgctgctcgc catgccggct ctcggccagc aggtgccgtc gacgaccatc gacgagctgc
    10141 ggcgcgacct cgaggcgctg acgcagcggc tcgagcgcgt cgaggcggaa aacgcctatc
    10201 tgaaggacaa tgcccgggcc aatcgcaagg agatcgccga ggccgatgtc gcgctcgcga
    10261 ggctgccgga catcgagaag gccggcaagg ccgccgactg ggcgggcagg atcaagctga
    10321 agggcgattt ccgctatcgc cacgaaaaca tcgacgacgc gacggccgcg aaagaccgca
    10381 cgcgccaccg cgtgcgcgtg cgcctcggcg tcgaagcgaa ggtcaacgac gccgtcaagg
    10441 ccgtcgtgca ggtcgccacg gccggtgacg accgagatcc ccgttcgacc aaccagacgc
    10501 tcggcgaggg cttcacgcgc aaggggctcg gtctcgatct cgcctacgcc gactggcgcc
    10561 cggtgcaggg tctcaacgtg cagttgggca agatcccgta cgcgtggcag cgcgtcggca
    10621 gctacttctg ggacggcgac ctgacctggg aaggcggcgc ggtcaggttc gcgcgcggtg
    10681 cattcttcgg cagcgcgatg ggcgcctggc tgtccgagtc cagctcgggc gccgatgcca
    10741 acgtgttcgg tggccagatc ggcgtgaaga cgaggctcgg cgacgcgatc ggcttcaccg
    10801 gcgcagtcgg atatttcgac gtcagcgccg tgcagaacga gatcacgctg acgtcggcga
    10861 cgccggcctg cgccgccaac aaggtgttct tcggcggcgc gcaaggcaac cggaccgacg
    10921 tcgtcgccgg ctgccgtcgc ctgcgcgacg actacaacat gatcgaggcg ctggcgcagc
    10981 tcgacttcaa ggtcggcgat ctgccgctcg cggtgttcgg caactacatc aggaaccagg
    11041 aagcggccga caacgacacg ggctacgcct tcggcgcgac gctcggcaag gccggcgacc
    11101 cgcggacctg ggagctgagc tatgtctacc aggacatgga gcagaacgcg cagttcgcgc
    11161 agttcgtcga ttcggacttc ggcgcgggca ccacgttctc gaagggcagc gtcgtcaagg
    11221 cggcctgggc gccggcgaag aactggaccg tgaacggcac ctacttcttc aacgagcgcg
    11281 tgccgccggg cgccgcggcg gtcagcgaga cctggcggga ctatgaccgc ctgcagctcg
    11341 acctgaacta caggttctga tcgtccgctg tcacgcaacc gtcatacacc gctcttaaca
    11401 tcgcatccgt acatcgagga ccctgaaatg aaactgacat cgttgttcac gctgatcgcc
    11461 gcaggcattg cggcgctcgc cttcgcgcct ggcgacgcgt cggcccagtc ggtcgtcaag
    11521 atcgacggct cgagcaccgt attcccgatc tccgaggcgg tcgccgagga attccagatc
    11581 gcaagacgcg gcaaggtgcg cgtcacggtc ggcatttccg gcacgggtgg cggcttcaag
    11641 aagttctgcc gcggcgagac cgacatctcg gacgcctcgc gcccgatcag caccggcgag
    11701 atggcggcct gcgcgaaggc gggcgtccag tacatcgagc tgccgattgc ctatgacgcg
    11761 ctgacggtcg ctgtgaatcc gaagaacacc tgggtcgatt cgatcacggt ggaagagctg
    11821 aagaaaatct gggagcccgc cgcacagggc aagatcacaa cctggaagca ggtcaatccg
    11881 aaatggccgg cgcagccgct caagctgttc ggcccgggtg ccgattccgg cacgttcgac
    11941 tacttcaccg aagcggtggt cggcaagtcg aagtcgagcc gcggcgactt taccgcgagc
    12001 gaggacgaca acgtgctcgt gcagggcatc gagtcggacg tcaatgcgct cgcgtacttc
    12061 ggctatgcgt actacgcgcc gaattcttcg aagatcaagg cgctcaggat cgtcaactcg
    12121 aagggccagg cggtcggccc gtcgccgcag ggcgtgctcg acggcagcta cgagccgctg
    12181 tcacgcccga tcttcatcta cgtgaacaag gcgtcggcgc agcgtcccga agtgagggat
    12241 ttcgtcgagt tctatctgca gaagggcgcg cctctcgtcg aggaagtgaa gtacgtgccg
    12301 ctcgcgaagc ccgcctacga caaggcgctg gccaatttcc gcgccggcaa ggccggcacc
    12361 gcgttcggcg gccacaacga ggtgggcctg acggtggacg agctgcttgc gcgcgaagcg
    12421 aagttctaat ctactggccc ggccgtgact gcccattctt ccagccctgc gtcgctgcgt
    12481 cgttcgatcc ggcggcgcag ggtccgggac cgcctgatcg aggcgctgct gctggccgcc
    12541 gggctggtcg cggtgttcac gacgctcgcg atcgtgtgga tcctggtcgt cgaggcgctg
    12601 ccgttcttcg agcacgtgcc gctgcgcgat ttcctcaccg acacgatgtg gaccccgctg
    12661 ttcgccgagc cgcgctacgg catcctgccg ctcgtcgccg gcacgctggt caccaccttc
    12721 gtggcgctgc tggtggccat ccccgcgggt accatcatcg cgatctacct ctccgagttt
    12781 gccagccacc ggctgcgcga gaccgtcaag cccgcgctcg agatgctcgg tgccgtgccg
    12841 acgatcgttt acggctactt cgcgctggtg atggtcacgc cgttcctgca gaagttcttc
    12901 cccgggttgc cgggcttcaa catgctatcg gcaggtctcg tgatcggcag catgatcgtg
    12961 ccctacgtcg cctcggtcag cgaggacgcg atgcgcgcgg tgccgaacta catgcgcgag
    13021 ggcagctacg ccaccggcgc cacgcgcttc cagacggcga cacgcgtcgt ggtgccggcg
    13081 gcgctctcgg gcatcgctgc ggcctatatc ctcggcattt cccgcgccgt cggcgaaacg
    13141 atggtggtcg cggtagccgc gggcctgcag ccgaatctca cctggaaccc ggccgagtcg
    13201 gcggcgacga tcacggcgtt catcgtgcag gtgagcctcg gtgacctgcc gcacgacagt
    13261 atcggctacc agtcgatctt cgcggcaggc ctggtgctgc tgctgatcac gctcgtgttc
    13321 aatattgccg ggttcgcgct cacgcgccgc ttcagggagg cttactgatg gccgtggtca
    13381 ccgaggatcc gtcgatccac gctctgcgcg ccgatgtttc gcggcgcaag ctgttcgacc
    13441 tggccttcgt gatcatcggg ctcgtcgtgc tgttcgcgtc gctgctggtg ctgctcgtgc
    13501 tgttcctcga cctggtaatg cagggcgccc cccgcctcaa ctgggacttc ttcacctcgt
    13561 acgcctcgcg caagccggag aatgccggca tcctcgccgc gtggatcggc acgacgctga
    13621 tcatggtggt taccgcctgc gtcgcgatgc cgatcggcgt cgccgcggcg atctacctcg
    13681 aggagtacgc gccgaagaac tggttcacct cgatcatcga gatcaacgtc accaacctcg
    13741 cgggcgtgcc gtcgatcatc tacggccttc tggcgctcgg cctcttcgtc tacaagttca
    13801 gcctcggcca gagcatcgcg gccgcgggcc tgaccctcgc gctgctgatc ctgccgatcg
    13861 tgattgtcgc gacgcgcgag gccgtgcgtg ccgttccgcg aacgatccgc gaggcggctt
    13921 acgcgctcgg tgccacccgc tgggaagtca ccgcgcacca cgtgctgccc aactcgatgg
    13981 gcggcgtact gaccggcatg atcatcggcc tgtcgcgcgc aatcggcgag acggcgccga
    14041 tcatcacgat cggtgcactg tcgttcatcg cgttcctgcc gagcccgccg ctcacggccg
    14101 agttcccgtt cctgtccttc gcatggctga gggattcatt cacggccatg ccgatccaga
    14161 tgttcaactg ggtctcgcgc ccggatcccg gctttcgcgt caacgccgcg gcggccggcg
    14221 cgatcctgct cggcatgacc ctgctgatga acggcgtcgc gatctggctg cgctaccgct
    14281 tccgcaagag gatcaactgg tgaccgagaa tcaagtgcag gctgccacgg gggcggccgt
    14341 ggcagtgtcg ccgaagagcg ggggaggccg tgtggagacc gaaaggctca aggtggccgt
    14401 atacggcgaa cgggcggctg cggccggcaa ttcggtcaag gcgcgcgcca gcaagatgag
    14461 cttctattac ggcagcgtgc aggcactcaa gagcctcgac atggagatca gcgacaagca
    14521 ggtgactgcg ctgatcggcc cctcgggctg cggcaagagt acttttctgc gctgcttcaa
    14581 tcgcatgcac gacctgactc cgatgacgcg ctacgagggc tcgattcgcc tctttcccga
    14641 cgacatcgac ctgatcgcga aggacgtcga cccgatcgaa gtgcgcatgc gcatcggcat
    14701 ggtgttccag aagccgaacc cgtttccgaa atcgatcttc gaaaatgtcg cctacggcct
    14761 gcgcgtgcgc ggcatgcgct ccaaggcgca gatcgaggag caggtcgaga aggcgttgcg
    14821 cgaggcggcg atctgggacg aggtaaagga ccgcctgcat acctccgggc tgtcgctgtc
    14881 cggcggccag cagcagcgcg tgtgcatcgc gcgcgcgctg gcgacgaatc cggaaatact
    14941 gctgttcgac gagccgacct cggcactcga cccgatcgcg accgcgcgca tcgaggaact
    15001 gatcaccggc ctgcgcggcc gcgtgaccgt gctgatcgtc acccacaaca tgcagcaggc
    15061 cgcgcgcgtg tccgactaca cggcgttcat gtatctcggt gagctggtcg agttcgacgt
    15121 cacgagcaag ctgttcacga acccggccaa gaaagccacc gaggactaca tcaccggccg
    15181 ctacggctga gcgccgcgcc gatggaaccg gcagcgactc tcgacgtggc gatgtcgtcg
    15241 ctgcggctga aggtcctcga gatgggcgga ctcgtggtcg atcaggtcac caccgcggtg
    15301 cgcgcgctgc tcgagtacga ccgcgacgct gccgaactcg tgctggcccg ggagcgccag
    15361 gtcaacgcct acgacacggc gatcgaggcc gccggcttcg agctgctcgc gctccgcgcg
    15421 ccggtcgcga cggacctgcg aggcgtactg gcgatcgcgc atgtggtgac cgacctcgag
    15481 cgcgtgggcg acgaggccaa gcgcatcgcg cgctttacgc tggcggcgat ggcccggccg
    15541 ggctttgatc cgtcggtggc cgtgcaccgc cacctgcgcc acatggcgca ccagtcggcg
    15601 cgcatgctgc gcatcgcgat cgacgccctc gacaagggcg acgccacggc ggcgcaccag
    15661 gtcgcccgca tggatcgcga gatcgaccgc gaattcgacg ccgcattgcg cgagttgctg
    15721 actgtcgcga tggaaggccc cacgcagctg cgcggcacga tcgataccgt gtttgcgctc
    15781 aagagcctcg agcgcatcgg cgatcacgcc aagaacgtgg ccgagcgggt cgtgttcatc
    15841 gtcagcggcg aggatgtcag gtacatgcgc gcgaaccacg acggccgcgg ctagccccgg
    15901 gccccgacgg ccagccctca gtcgtcggtg cgcagcaagt cgttgaggct ggtcttcgcg
    15961 cgcgtcttcg catccacttt cttgacgatc acggcgcagg cgagactgtg ggtgccgtcc
    16021 gtcgagggca gggtgccggg caccacgacc gagccggccg gcacgcgccc gtgcagcacg
    16081 atgccggttt cacggtcgta gatgcgggtg ctctggccga ggaacacgcc catcgagatc
    16141 accgagccgc gctcgacgat caccccctcg acgacttccg agcgcgcgcc gatgaaacag
    16201 tcgtcctcga tgatggtcgg cgccgcctgc agcggttcga gcacgccgcc gatgccgacc
    16261 ccgcccgaga gatgcacgtt gcggccgatc tgcgcgcagg agccgacggt cgcccatgtg
    16321 tcgaccatgg tgccggcacc gacatgcgcg ccgatgttga cgtaggaggg catcagcacg
    16381 acgttcggcg cgacgtaggc gcccttgcgc acgatggccg gcggcacgac ccgcacaccg
    16441 ctcgcgcgca ggctcgcctc gtcgtcggtc gcgaacttga gcggcacctt gtcgtagaag
    16501 cgggcgtagc cggcgtcgac gaccgtgttg tcatgcgtgc ggaaataaag gagcaccgcc
    16561 ttcttgagcc actcgttgac gacccagtgg cggccgtcgt gttcggcgac gcgggcctcg
    16621 cccgaatcga gcagccctat ggcgcgctcg atcgccgcgg ctacgtcggc gggcaccttt
    16681 ccgggggaca gttcggcgcg atgctcgaat gcgtcgtcga cgattgtcct gagtgcggtg
    16741 tggtcggtca tggcacgctc ctgcggggga gcgcgcatcg tgccgggctc agccggacac
    16801 tgcaacctgg ccgggcgcgg cttgctttcc tgcatccaca tcggcatgca atcccgcgag
    16861 caccgtatcg agcggcaccg gcgcgccgaa ggcatagccc tgtccgtaag tcacgccgag
    16921 cgaacgcagg cgatccgcga tggcggtcgt ttcgacgtat tcggcgaccg tgtcgagtcc
    16981 cagttgcgag gcgatctgca gtaccgcgcg gatcacgccc tccgagcgct cgtcggtgag
    17041 caggtcgcgc acgaacgagc cgtcgatctt cagggccgag accggcaacg acttcaggta
    17101 ggcgagcgag gagaagccgg tgccgaaatc gtcgagcgcg aagtgtgcgc ccagcgcgcc
    17161 gacccgctcg atgaagcgct gcgccgctgc gatgtc
//
LOCUS       DAOQVY010000045        17118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_3810_length_17118_cov_6.152611, whole genome shotgun sequence.
ACCESSION   DAOQVY010000045 DAOQVY010000000
VERSION     DAOQVY010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17118
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3810_length_17118_cov_6.152611"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..1008
                     /locus_tag="PKN91_06045"
     CDS             214..1008
                     /locus_tag="PKN91_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNS27668.1"
                     /translation="MVAKRKAEADPSASSQLTIGQLARRAGVSTSMLRFYEREGLLPA
                     PARSPSGYRLYPRTALRSLLFIHRAQRLGFSLGDIRLLLAGESGEGGRGTSVVGIAEQ
                     RFLEIERRLTELLVLRHELELFLDDLTRRVGHAGGDDVARVYRRLVDHVCGHGPQRAA
                     PSTMYGLIKRLGCALASVEQDKVFAALRGRHLHVWKENDGYSILVRDRGAAVREALEK
                     LVLAEAGCRVHVDVSLAPTPDGLLLRARGDNAFLIAQLFLALEAAT"
     gene            1043..3346
                     /locus_tag="PKN91_06050"
     CDS             1043..3346
                     /locus_tag="PKN91_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNS27669.1"
                     /translation="MESAATRLKIAGMDCGSCALTIEDGIRKLPGVRAVSVDFATESM
                     RIEGDVALEVIENRLTQLGYRLQGESAAGAAPAAASVGLEHRGLAGFLRFLWSQPRLR
                     IASIATLVVLAATALALAAPVVMPARALSLVFLLTVLVTGLPVFGKGLRALLYSHRIT
                     IDLLMAIATLGALAIDAGGEAVTVILLYTLGEALEAYSADRARDSLRSLLALQPQTAT
                     VMRLHRASHEAAAKHEHSAHAHSAHDHEAGHEHYHHETVPVDAVTVGARLLVRPGERV
                     PVDGRVVGGASSLNEAAVTGESMPVAKDVDDTVLAGTVNGEGALEIEALRVAGDSTIA
                     RIARLVEQAQAERSPAERFIDRFARWYTPAVVLLAIAVVLVPVLAFGQPLLDHAGGHG
                     WLYRGLALLIVACPCALVISIPVAVVSSLTRLAQLGVLVKGGERLDALADVSAVAFDK
                     TGTLTSGVLAVSAFAGRDCVHPDTLNGDCAACDDVLALAAAVEQSSEHPVSRAIRDAA
                     QLRGVAHRHPQAVAVRALPGRGIVGEVGGHRVAIGGEALFAEPDLALPVPEQLASVLR
                     DAERTTMLVARDAEFVGLIAVEDQPRAGISAALEALQRAVPGLRTIMLTGDNPRVAAR
                     VARAIPGIDEVRAGLLPADKMRELELLKARHGEVAMVGDGINDAPALARADVGIAMGA
                     AGTAQAMESADVVLMQDDLGILPRAFAIARRSRRLVKQNIVLSLGLKLAFLAITIPGW
                     ATLWLAVAADVGATLLVTMNGMRMLRA"
     gene            3391..4695
                     /locus_tag="PKN91_06055"
     CDS             3391..4695
                     /locus_tag="PKN91_06055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS27670.1"
                     /translation="MTNETPRRLAFIELAPGVLPRHALAYLFAAFVSIGLFAYLSAMT
                     PFILSVNLSLPEAQHGRASGLLQGWQEVIILLVIGWWGALSDRIGRRPVYVGGFLLIS
                     IAYFLYPLADSLPELVAYRVVFGCAVAALGAMLATVLADYPRESSRGKLSGVAFFLNA
                     LGSVVFLLGLTQLPRLYAAAGASQLEAGRYAFFTIGAVALLAACVMFGLKPGLPTQVK
                     SRKPMLALIREGMQAARNPRIALAYGGGFISRADLSLITLFLALWAAQSAQQEGLSAA
                     QAAGRVGITIGVTQLTAMVWAPVYGWLADHMNRTVHFAGAFALALVGYGWVGMQDDIV
                     ATSALPALIMLGVGQSSTILAATILLGQEAPRESRGSVFGLQAFCGGLGILAISWAGG
                     LLYDHVAPSAPFTLVGVANAVVALWAVWVYLRSRRAPREESA"
     gene            4739..5614
                     /locus_tag="PKN91_06060"
     CDS             4739..5614
                     /locus_tag="PKN91_06060"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNS27671.1"
                     /translation="MGPRRPAWWIALLARIPLGALYGLAWLLGWLGFHVFRYRRGIID
                     ASLARAYPDLDAKARADICNRHHHGFSQVFVEIVKSGAMTPAEIARRVRFTNLALLEE
                     RLGRGRPVLVLAAHQCNWEWLMHAMASQSGFPVDAAYKPLKNPWANREMLTLRTRFGA
                     HLVPAKELLSEIIKRRAIVRTIAMIADQEPRSSEKRHWTRFLNRDTAFYMGAEEIARK
                     TGYAVLFAGMRRLSRGHYEVSFTPLVEAGERLEPGEFTERYARLTEAQINASPPDWLW
                     SHKRWKLKKPVYGAG"
     gene            complement(5599..7563)
                     /locus_tag="PKN91_06065"
     CDS             complement(5599..7563)
                     /locus_tag="PKN91_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HNS27672.1"
                     /translation="MTQPQTLTELIESNRNVARSIIYLEGEKDERRLSYGELHERALG
                     ILFHLQALGARPGDKLILFLNNNEQFLDAYWAGILGGIVPVPVSIGISDEHRHKLLRI
                     ARKLGKPFLYTDSKTLDRIGQFAVGAGEDATFEALRPRTFLADRLEDISRAGTVHRAQ
                     PQDTAFIQFSSGSTNEPKGVVLTHANILANSRGAGEHCGFNTDDVSLSWMPLTHDMGL
                     IGMHIFMFMHRMNVHLMPTELFVRRPLLWLQFASRIGATITSSPNFGYRHYLKVLGER
                     SFEDLDLSRLRLIFNGAEPISVDLCEEFLARMEPAGLARKAMFPVYGLAEASLAVSFP
                     PMGRLYRSICVDRHRMGVGEAPPRVEHTHRDAMELMAVGNAIPYCEVRLADDAGARVA
                     DGHVGHILIRGENVTHGYFEDPEATAAAITADGWLRTGDLGMLVDGDLYITGRAKEII
                     FVNGQNYFPHDLESIALRAPGVELGKLVVAGVRPRGAQADQLVAFLLHRGDLADFLPT
                     ATEVARLINEHTGLEVAEVVPVKRIPKTTSGKIQRHLLEESYVDGELAAELAELARLR
                     SEQRPPAAASGSEIEAKIKAICDSELGRPIGLDDNLFEIGASSLKLIAIHESIDRDYP
                     GLIDLTELFDYPTIRELAAFLAGKLAQPAP"
     gene            complement(7560..8876)
                     /locus_tag="PKN91_06070"
     CDS             complement(7560..8876)
                     /locus_tag="PKN91_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNS27673.1"
                     /translation="MNETIPANCLPPEADEPFDIGATDDSLARMSELFATHGDIYRVY
                     APSRRAHTWLTIHPDDVKRVLVSNHRNYVKGIGFDRVRILLGNGIVTSDGELWKRQRY
                     MMQPMFHRRVLTQFGRMIDEANDRFLAGWAAQAGSGRPVNVTNDMSALALEVILHALF
                     GKDLAVLAAMPGGNPFDLFTDESARDLRFAYRFRQLTREIAQLIERRRSEPPEAHFDF
                     LGMLMEARDRETGAGMTDREMIDEVTTLIVAGHETTANTLNWAWYLISQHPQVEARMH
                     AEIDAMPEESAPGLARMEELTYTKCIIDETLRLYPPVWVFSRRAVGPDVLSGHALPAG
                     ADLLLSTYHLHRHPRYWRDPDAFMPERFDAAHEAEWPRFAYMPFSAGPRHCIGETFSI
                     FEMLIHLSKVGRRYRLRFEHEGPIEYEAQVNLRTRHPLLMTLEPRS"
     gene            8892..9437
                     /locus_tag="PKN91_06075"
     CDS             8892..9437
                     /locus_tag="PKN91_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27674.1"
                     /translation="MVRGILYYSTLPPDAGGDLSQAGIALALACIDALSSGAPAARAE
                     SLLFPPRGKPWMPGAPQFSVSHSAPVVACAAVSRGDVGLDVECDAAADRLTLSTICDD
                     DELELARRIGIRELWVAKEAALKAGGGTIEQIAGVRVDAGGASFRGVRYHAHRIALDA
                     QCPACIMTSEPHVQFEVRRHA"
     gene            9434..10138
                     /locus_tag="PKN91_06080"
     CDS             9434..10138
                     /locus_tag="PKN91_06080"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNS27675.1"
                     /translation="MTGKGSRRRMTPLRRLAYRIIVPIGAALIRLVWGVARRPRVVGG
                     EHLDAALAIAPSFIPVLWHQHQLFCAKWLLEQRPRVTLGFLISPSVDGEIGAMLVDRF
                     GGVAVRGSSTHTGARALRDYYEALVKRDVSPVITPDGPRGPRFVFKPGAILLAQMSGR
                     PMLPMAYAASRAILFHWDRFVIPLPFSHIVIAVGAPRCVPRTLGAAGMQQWQEDMTAE
                     LKSLYARAHAALPVRD"
     gene            10495..13065
                     /locus_tag="PKN91_06085"
     CDS             10495..13065
                     /locus_tag="PKN91_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNS27676.1"
                     /translation="MCTKIAVDLQNMQRETLAAVLARSLESLREATACDAAFVAMFND
                     EAVTELHVARGTFAQTRLEELRGVALESLPWLKSRLEHLRLSELRDTAAPRKENAVES
                     RLFASLKVGSTLYVAFRALGRPAGILGLANAMSRGPWDVNVQLLLKLLGTSLATGLDR
                     VRLRTRLDDLEVRVGLAQAAANEGMWDFDTETNVVSFSPRWKEMLGYSDEDLSGPTDW
                     RALVHPDDMSRVQTAMREHVEGHAPICESIHRMRHRSGEWRWVVSRAKAHVDENGRLL
                     RLVGMEFDITERKLYEEALFREKESAQITLQSIGDGVITTDANSIIDYINPIAEELTG
                     WRLEDAMGRSVDDVFKAFHEETCEPLENPLTMSVRRIRPIKSVRPMLLIRRDGNELYV
                     ESTAAPIRDGAGKVCGGVLVFHDVSESRELNRRLSYHASHDILTGLVNRREFENRLER
                     ALKSARAREASYALCYLDLDQFKIINDTCGHSAGDALLGQVGALLKSKVRWRDTLSRL
                     GGDEFGILLESCSLDEAMRTAETLREALRNFRFTWEDRVFRLGASIGVVPITADNEDV
                     ASVLSAADSACAAAKEAGRNRVHSFAENDIELMRRRREMQWAARINSALEEGRFELYR
                     MTIQPLQPSSLIQGAHYELLLRMRDENGRMVSPDNFIAAAERYGITPNIDRWVIENAL
                     RWLVSEADERERLAMCSINLSGQSFSDDKFLPFVIDQFARSGIDASKICFEITETAAV
                     ASFSQANRFIQALKELGCKFALDDFGTGLSSFGYLKHFPVDFLKIDGSFVREILRDPI
                     DREMVRSINEIGHLTGKQTIAEFAENAEIIQMLTGLGVDYAQGYGIAQPQRVLKAVNA
                     "
     gene            13141..15174
                     /locus_tag="PKN91_06090"
     CDS             13141..15174
                     /locus_tag="PKN91_06090"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNS27677.1"
                     /translation="MGFNRTGLLSAAALCMAAIPAPAADRYDIIFAGNLSGQQTTAVA
                     ADGSVGSTLSWRNNGRGPDIEERIVVAADGTLASYRATGTSTFGSKIDETFSRRGDRV
                     VWRSSADRGDRRVDGPALYLPVEGSFEATAVIARALLAAPGGRLAALPGGELSIERLA
                     ATALEQGGERRAVALYAIHGMSTQPRFLWLSDDDERRFFALVYPGYMLVAPSGWSQVA
                     AALEALQKDAEQKLLEALAARLAHRLGDELVIRNARVFDSDTGALTAPQDVYVHDGRI
                     AALYPAGSRPSGAMPAIDADGRALLPGLFDMHVHDDAWNGLLHLAGGVTTVRDMGNDN
                     RLLRELAVKGEAGRLAAPRIIAAGFIEGESPHAARADFVVGDLEAAKAAVDWYAQRGY
                     LQVKLYNSIRPEWMAPIAQYAHERGMRVSGHVPAFARAEDAVRAGYDELQHINQVVLN
                     FLVMPDTDTRTLARFYLIGEQAADIDLDSREVRDFIALLRERGTVLDPTLATFEYMFQ
                     QARGQPNPTFAAVADHVPPALAREWGTNVMDIPPAKVTRYHSGYEKMLGFLRLAHEAG
                     ITILAGTDDIGGFTLHRELELQVKAGIAPADALRIATRNGARVLGLWDDSGSIERGKR
                     ADLILVEGDPTRDISAIRRIGLVMKAGVAYFPAEIHEALGVRRFADPPKVTLP"
     gene            complement(15184..16062)
                     /gene="nadC"
                     /locus_tag="PKN91_06095"
     CDS             complement(15184..16062)
                     /gene="nadC"
                     /locus_tag="PKN91_06095"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNS27678.1"
                     /translation="MHGRRHARSAVLTELPHNLEEQVAAALREDVGGGDVTAALVPER
                     ELAQAQVVAREAAILCGTAWFDAVFRALDPRLRIDWYAADGERVGRDTVLCRIEGPAR
                     PLLTGERTALNFLQTLSGTASAARRYVDAVAGTGCTILDTRKTVPGLRLAQKYAVRCG
                     GAQNHRLGLHDMVLIKENHIAAAGSIPVAIATARRLSPGIPIEVEVENAAELEHALAA
                     RPDVVMLDEFSLDGMHAAVARNRELGRPVKLEASGNVSLATIRAVAETGVDYISVGAI
                     TKHVHALDLSLRITRG"
     gene            complement(16026..16637)
                     /locus_tag="PKN91_06100"
     CDS             complement(16026..16637)
                     /locus_tag="PKN91_06100"
                     /inference="COORDINATES: protein motif:HMM:NF017945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HNS27679.1"
                     /translation="MKILSNHRYALVTGVVLALILAYIINAVVSDPIAYHNLGLARWV
                     HIVAGVFWIGLLYYFNAVQTPALAAATADKGGPGGAGISKYVAPRALFWFRWSALVTW
                     LSGAWYLGARFPGAFSLGLTSNPVDYYLLVIGIGAWLGTIMLFNVWVLIWPNQKKVLG
                     IVAATDEQKAAARVIAGNTSRVNFILSFPLLMCMAGATHGLPF"
     gene            16753..17070
                     /gene="grxD"
                     /locus_tag="PKN91_06105"
     CDS             16753..17070
                     /gene="grxD"
                     /locus_tag="PKN91_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HNS27680.1"
                     /translation="MDVVDRIKQHLAAPVVLFMKGTPDFPQCGFSAQVAAALRAVGAK
                     YEAVNIFEDLELRDELKRYSNWPTYPQLYVNGELIGGCDIVLDLYRSGELANILAAAG
                     KAA"
BASE COUNT         2635 a         5688 c         5967 g         2828 t
ORIGIN      
        1 caggttgtcg ccgcccacca tcgcgtcgat cgcgcgcccg atgaaccagt acagggtcag
       61 gctcgccagc agcccgagca cgccggcgcc gatgccgatg ccgagccatc ggcgcgtgga
      121 cgccaccatc agccgcttgt cgatcccgag cacagtcacc cccgtgattg ctgggcagat
      181 cctgcacctt gaagtagagt tcaaggtcaa cacatggtag cgaaacgcaa ggcagaagcg
      241 gatccgtccg caagctcgca actgaccatc ggacagctcg ccaggcgcgc cggcgtcagc
      301 acctcgatgc tgcgcttcta cgagcgcgag ggcctgctgc cggcgcctgc gcgctcgccg
      361 tccggctacc ggctgtatcc gcgtaccgcg ctgcgctcgc tgctgttcat ccatcgcgcg
      421 cagcggctcg ggttttcgct cggcgacatc aggctgctcc ttgccggcga atcaggggag
      481 ggcgggcgcg gcacgagtgt ggtcggcatc gccgagcagc gctttctcga gatcgagcgc
      541 cggctgaccg aattgctggt gctgcggcac gaactcgagc tgttcctcga cgacctgacc
      601 aggcgggtgg gccacgccgg cggcgacgac gtcgcgcgcg tttaccgccg gctcgtcgac
      661 catgtctgcg gccacgggcc gcagcgggcc gcgccctcga ccatgtacgg gctgatcaag
      721 cggctcggct gtgcgctcgc ctcggtcgaa caggacaagg tgttcgcggc actgcgcggc
      781 cgccacctgc acgtctggaa ggaaaacgac ggctactcga tcctcgtgcg tgaccgcggt
      841 gcagccgtgc gggaggcgct cgagaagctg gtgctcgccg aggcgggctg ccgcgtgcac
      901 gtcgacgtga gcctggcgcc gacgcccgac ggtctgctgc tgcgggcacg cggcgacaac
      961 gccttcctga ttgcacagct gttcctggcg ctcgaggctg cgacttgacc ttgaagcagg
     1021 gttcaaggtg caggatggcc gcatggaaag cgccgccacc cgactgaaga ttgccggcat
     1081 ggactgcggc agctgcgccc tcacgatcga ggacggcatc cgcaaactgc cgggcgtgcg
     1141 cgccgtcagc gtcgatttcg ccaccgagtc gatgcgtatc gaaggcgacg tggcgctcga
     1201 ggtcatcgag aatcgcctca cgcagctcgg ctatcggctg cagggcgaga gcgcggcggg
     1261 cgcggctccg gcggccgcca gcgtcggact ggagcaccgc gggctggccg gcttcctgcg
     1321 cttcctgtgg agccagccgc ggctgcgcat cgcgagcatt gccaccctcg tcgtgctcgc
     1381 cgccacggcg cttgcgctcg cggcgcccgt cgtaatgccg gcacgcgccc tgtcgctggt
     1441 cttcctcctc accgtgctcg tcaccggcct gccggtgttc ggcaaagggc tgcgcgcgct
     1501 gctctacagc catcgcatca cgatcgacct gctgatggca atcgccacgc tcggcgcgct
     1561 ggcgatcgac gccggcggcg aggcggtgac cgtgatcctg ctgtacacgc tcggcgaggc
     1621 gctcgaggcc tacagcgccg atcgcgcccg cgattcgctg cgcagcctcc tcgcgctgca
     1681 gccgcagaca gcgacggtga tgcgcctgca ccgtgcgagc cacgaggccg cggcgaagca
     1741 cgaacactcc gcgcacgccc attcggcgca cgaccacgag gccggacacg agcactatca
     1801 ccatgagacc gtgccggtcg atgcggtcac ggtgggcgcg cggctgctgg tgcggcccgg
     1861 tgagcgcgtg ccggtcgacg gccgggtcgt gggcggcgca tcgagcctga acgaggcggc
     1921 agtcaccggc gagagcatgc cggtggccaa ggatgtcgac gacaccgtgc tcgccggtac
     1981 cgtcaacggc gagggcgcgc tcgagatcga ggcgctgcgc gtcgccggag actccaccat
     2041 cgcgcgcatc gcgcgtctcg tcgagcaggc gcaggccgag cgctcgccgg cggaacgctt
     2101 catcgaccgt tttgcgcgct ggtacacgcc cgcggtcgtg ctgctcgcca ttgcggtggt
     2161 gctcgtgccg gtgctcgcct tcggtcagcc gctgctcgac cacgcgggcg gccacggctg
     2221 gctctaccgc ggtctcgcgc tgctgatcgt cgcctgcccc tgtgcgctcg tgatcagcat
     2281 tccggtcgcc gtcgtcagca gcctcacgcg gcttgcgcag ctcggcgtgc tggtcaaggg
     2341 cggtgagcgg ctcgatgcgc tcgccgacgt gagcgcggtc gccttcgaca agaccggcac
     2401 gctcacctcg ggagtgctgg ccgtgagcgc gttcgcggga cgcgactgcg tgcaccccga
     2461 tacgctgaac ggtgactgcg cggcctgcga tgacgtgctg gcgctcgccg cggcggtcga
     2521 gcagtcctcg gagcatcccg tttccagggc gatccgcgat gccgcgcaac tgcgcggcgt
     2581 ggctcatcgt catccgcagg cggtcgcggt gcgcgccctg cctggcaggg gcatcgtggg
     2641 cgaggtgggc gggcatcgcg tggcgatcgg cggcgaggcg ttgttcgccg agccggacct
     2701 cgcgctgccg gtgccggagc aactggcgtc cgtgttgcgc gacgcggagc gcaccacgat
     2761 gctggtcgcg cgcgatgccg aattcgtcgg cctgatcgcc gtcgaggacc agccgcgtgc
     2821 cgggatctcg gccgcgctcg aggcgctgca gcgggcagtc cccggcctgc gtaccatcat
     2881 gctcaccggt gacaatccac gcgtcgcggc gcgcgtggcg cgtgccatcc ccggtatcga
     2941 cgaagtgcgg gccggcctgc tgccggccga caagatgcgc gagctcgagc tgctgaaggc
     3001 gaggcatggc gaggtcgcga tggtgggcga tggcatcaac gatgcgccgg cgctcgcgcg
     3061 cgccgatgtc ggcattgcga tgggggcggc gggcactgcc caggccatgg aaagcgccga
     3121 tgtcgtgctg atgcaggacg acctcggcat cctgccgcgc gcgtttgcca tcgcgcgccg
     3181 ttcccgtcgg ctggtcaagc agaacatcgt actctcgctc ggcctgaagc tcgcattcct
     3241 cgcgatcacg attccgggct gggccacctt gtggttggcg gtggcggcgg atgtgggcgc
     3301 cacattgctc gtgacgatga atggcatgag aatgctgcgc gcctgagcag cagccgctga
     3361 cccggcgcca acgaaaaata cggggacagc gtgacgaacg aaacaccgcg cagactggct
     3421 tttatcgaac tggcgcccgg cgtgctgccg cgacacgcat tggcctacct ctttgctgcc
     3481 ttcgtcagca tcgggctgtt cgcctacctg tcggcgatga cgccgttcat cctgagcgtg
     3541 aacctgtcgc tgccggaggc gcagcacggg cgtgcctcgg gcctgctgca gggctggcag
     3601 gaagtcatca tcctgctggt gatcggctgg tggggcgcgc tgtcggaccg cattgggcgc
     3661 cggccggtct atgtgggcgg cttcctgctg atctcgatcg cgtatttcct ctatccgctc
     3721 gccgattccc tgcccgaact ggttgcctac cgcgtggtgt tcggctgcgc ggtggcagcg
     3781 ctcggcgcga tgctcgcaac cgtgctcgcc gactacccgc gcgagagctc gcgcggcaag
     3841 ctgagcggag tggcgttttt cctcaacgcc ctgggttcgg tggtattcct gctcgggctc
     3901 acgcagttgc cgcgcctgta tgcggcggcc ggcgcgagcc agctcgaggc gggccgctat
     3961 gcgttcttca cgatcggcgc ggtggcgctg ctcgccgcgt gtgtgatgtt cggcctgaag
     4021 ccgggactcc cgacccaggt gaagtcacgc aagccgatgc tcgcgctcat ccgcgaggga
     4081 atgcaggcag cgcgcaaccc gcgcattgcg ctcgcctacg gcggcggctt catatcgcgt
     4141 gccgacctga gcctgatcac gctgtttctg gccctgtggg ccgcacagtc ggcacagcag
     4201 gaggggctct cggcggccca ggcggccggg cgcgtcggca tcacgatcgg tgtcacgcag
     4261 ctcaccgcga tggtgtgggc gcctgtatac ggctggctcg ccgatcacat gaaccgcacc
     4321 gtgcacttcg ccggggcgtt cgcgcttgcg ctggtgggct acggctgggt cggcatgcag
     4381 gacgacatcg tcgcgacgtc ggcgcttcct gcgctgatca tgctgggcgt agggcagtcg
     4441 agcacgattc tcgcggccac gatcctgctc ggccaggagg cgccgcggga atcgcgcggc
     4501 tcggtgttcg gcctgcaggc gttctgcgga ggcctcggca tactcgcgat atcatgggcg
     4561 ggagggttgc tgtacgacca cgtcgcgccg tcggcgccct tcacgctggt gggtgtcgcc
     4621 aatgccgtag tcgcactgtg ggccgtatgg gtgtacctgc ggagcaggcg ggcaccgaga
     4681 gaggaatctg catgagttcg acgtattgtc cggtgggtct cggttgatgg tggaagccgt
     4741 ggggccacgg cgcccggcct ggtggatcgc attgcttgcg cggatcccgc tcggcgccct
     4801 ctacggattg gcgtggctgc tcggctggct cggcttccac gtctttcgct atcgccgtgg
     4861 catcatcgac gcgagcctcg ccagggccta tccggacctc gacgcgaagg cgcgcgcgga
     4921 catctgcaat cgccatcacc atggcttctc gcaggtattc gtcgagatcg tcaagtccgg
     4981 cgcaatgacg cccgcggaaa tcgcccgtcg cgtgcgcttc accaacctgg cgctgctcga
     5041 ggagcggctc ggtcgcggcc gaccggtgct ggtgctcgcc gcgcaccagt gcaactggga
     5101 gtggctgatg catgccatgg ccagccagtc cgggtttccg gtcgacgcgg cgtacaagcc
     5161 gctcaagaat ccgtgggcca atcgcgaaat gctgacgctg cgcacccgat tcggggcgca
     5221 tctcgtgccg gcgaaggagt tgctctccga aatcatcaag cggcgcgcca tcgtgcgcac
     5281 gatcgcgatg attgccgacc aggagccgcg cagcagcgag aagcggcact ggacccgttt
     5341 tctcaaccgc gataccgctt tctacatggg cgccgaggag atcgcgcgca agaccggtta
     5401 cgcggtgctg ttcgccggta tgcgccgcct gtccaggggc cactacgaag tcagcttcac
     5461 gccgctcgtc gaggcgggcg agcggctcga gccgggcgag ttcaccgagc gttacgcgcg
     5521 cctcaccgag gcgcagatca acgcctcacc gcccgactgg ctctggtcgc acaagcgctg
     5581 gaagctgaag aagccggttt acggcgccgg ctgagcgagc ttgccggcga ggaaggccgc
     5641 gagttccctg atcgtcgggt agtcgaacag ttcggtgaga tcgatcaggc cgggatagtc
     5701 gcggtcgatg ctctcgtgga tcgcgatcag cttgagtgaa cttgcgccga tctcgaacag
     5761 gttgtcgtcg aggccgatgg ggcggccgag ctcgctgtcg cagatcgcct tgatcttcgc
     5821 ctcgatctcc gagccgctgg ctgccgcagg cgggcgctgc tcgctgcgca ggcgcgcgag
     5881 ctcggcgagc tcggcggcca attcgccgtc gacgtaggat tcctcgagca ggtgtctctg
     5941 tatcttgccg ctggtggtct tcggaatgcg cttgacgggc accacctcgg ccacctcgag
     6001 gcccgtgtgc tcgttgatca gccgcgcaac ctcggtcgcg gtcggcagga aatcggccag
     6061 gtcgccacga tgcagcagga aggcgaccag ctggtcggcc tgcgcgccgc gcggacgcac
     6121 tcccgcgacc accagcttgc cgagctcgac gcccggcgcg cgcagcgcga tgctctcgag
     6181 gtcgtgcggg aaatagttct ggccgttgac gaagatgatc tccttcgcgc gaccggtgat
     6241 gtacaggtcg ccgtcgacca acatgccgag gtcgccggta cgcagccagc cgtcggcggt
     6301 gatcgcggcc gcggtcgctt ccgggtcctc gaaatagccg tgcgtgacat tctcgccgcg
     6361 gatcaggatg tggccgacgt ggccatcggc gaccctggcg ccggcatcgt ccgccagccg
     6421 cacctcgcaa taagggatcg cgttgcccac cgccatcagt tccatggcat cgcggtgggt
     6481 atgctcgacg cgcggcggcg cttcgccgac acccatgcga tggcggtcga cgcagatcga
     6541 cctgtacagg cgccccatcg gcgggaagga gacggccagc gacgcctcgg cgaggccgta
     6601 gaccgggaac atcgccttgc gtgcgaggcc ggcgggttcc atgcgtgcga ggaactcctc
     6661 gcacaggtcg accgagatcg gctcggcgcc gttgaagatc agcctgagcc tcgagaggtc
     6721 gaggtcctcg aagctgcgct cgccgaggac cttcaggtag tgccggtagc cgaaattggg
     6781 cgacgaggtg atcgtggcgc caatgcgcga ggcgaactgc agccacagca gcggtcggcg
     6841 cacgaacagc tcggtcggca tcaggtgcac gttcatgcgg tgcatgaaca tgaagatgtg
     6901 catgccgatc aggcccatgt catgcgtcag gggcatccac gacaacgaga cgtcgtcggt
     6961 gttgaagcca cagtgctcgc cggcgccgcg gctgttcgcc aggatattcg cgtgggtgag
     7021 caccacgcct ttcggctcgt tggtcgagcc ggaggaaaac tggatgaacg ccgtgtcctg
     7081 cggctgcgcg cggtggacag tgccggcgcg cgagatgtcc tcgaggcggt cggcgaggaa
     7141 ggtgcgtggg cgaagcgcct cgaacgtggc gtcctcgccc gcgccgacgg cgaactggcc
     7201 gatgcgatcc agcgtcttgc tgtccgtgta caggaagggc ttgccgagct tgcgcgcgat
     7261 gcgcagcagc ttgtgccggt gctcgtcgct gatgccgatc gagaccggca ccggcacgat
     7321 gccgccgagg atgccggccc agtacgcgtc gaggaactgc tcgttgttgt tcaggaacag
     7381 gatcagcttg tcgccggggc gcgcgccgag cgcctgcagg tggaacagga tgccgagcgc
     7441 acgttcgtgg agttcaccgt agctgaggcg acgttcgtcc ttttcgccct cgaggtagat
     7501 gatcgagcgc gccacattgc ggttcgactc gatgagttcg gtgagtgtct gcggctgggt
     7561 catgagcgcg gttccagtgt catgagcagg ggatggcggg tacgcagatt gacctgcgcc
     7621 tcgtattcga tcggaccctc gtgctcgaag cgcagccggt agcggcgccc gaccttggag
     7681 aggtggatca gcatctcgaa gatggagaag gtctcgccga tgcagtggcg cgggccggcc
     7741 gagaacggca tgtaggcgaa cctcggccac tcggcctcgt gcgccgcgtc gaagcgttcc
     7801 ggcatgaacg catccggatc gcgccagtaa cgcgggtggc gatgcaggtg gtaggtcgag
     7861 agcagcaggt cggcaccggc cggcagcgca tggcccgaga gcacgtcggg gccgacggcg
     7921 cgccgcgaga atacccatac cggcggatac aggcgcagcg tctcgtcgat gatgcacttc
     7981 gtgtacgtga gttcctccat gcgcgcgagg cccggtgcgc tctcttcggg catggcgtcg
     8041 atctcggcat gcatccgcgc ctcgacctgc ggatgctgcg aaatcaggta ccacgcccag
     8101 ttgagcgtat tcgcggtcgt ttcgtggccg gcgacgatca gcgtcgtgac ctcgtcgatc
     8161 atctccctgt cggtcatgcc cgcgccggtt tcgcgatcgc gcgcctccat cagcatgccg
     8221 aggaagtcga agtgcgcctc gggcggctcg ctgcggcggc gctcgatcag ttgcgcgatc
     8281 tcacgcgtga gctggcggaa gcggtaggcg aacctgaggt cgcgcgccga ctcgtcggtg
     8341 aacaggtcga acgggttgcc gcctggcatg gccgcgagca cggcgaggtc cttgccgaag
     8401 agggcgtgca ggatgacctc gagcgcaagc gcgctcatgt cgttcgtgac attgacaggc
     8461 ctgccgctgc cggcctgcgc cgcccagccg gcgaggaagc ggtcgttcgc ctcgtcgatc
     8521 atgcggccga actgcgtcag cacgcggcga tggaacatgg gctgcatcat gtagcgctgg
     8581 cgcttccaga gctcgccgtc gctcgtcacg atgccattac cgagtagaat cctgacccga
     8641 tcgaagccga tgcccttgac gtagttgcgg tggttcgaaa cgagcacccg cttgacgtcg
     8701 tccgggtgga tcgtgagcca ggtgtgcgca cggcgcgagg gcgcgtagac gcggtagatg
     8761 tcgccgtgcg tggcgaacag ctccgacatg cgcgcgagcg aatcgtcggt tgcgccgatg
     8821 tcgaagggct catcggcctc gggcggcagg cagttcgcag ggatcgtttc gttcacgaag
     8881 cggggcagtc aatggtgcgc ggaatcctat attactcgac attgccgccg gatgcgggcg
     8941 gcgacctgtc gcaggccggc atcgcgctcg cgctggcgtg catcgatgcg ctgagttccg
     9001 gtgcgcctgc ggcccgtgcc gagtcgttgt tgtttccgcc gcgcggcaag ccgtggatgc
     9061 ccggtgcgcc gcagttcagc gtgtcgcatt ccgcgcccgt ggtcgcctgc gcggcggtgt
     9121 cacgcggcga cgtcgggctc gacgtcgaat gcgacgccgc ggccgaccgg ctcacactct
     9181 cgacgatctg cgacgacgac gaactcgaac tcgcgcggcg gatcggcata cgcgagctgt
     9241 gggttgcgaa ggaggcggcg ttgaaggcgg gcggcggcac gatcgaacag atcgccggcg
     9301 tgcgcgtgga cgcgggcggc gcgagcttcc ggggcgtgcg ctaccatgcc catcgcatcg
     9361 cgctcgatgc gcagtgtccc gcctgcatca tgaccagtga gccgcacgtg cagtttgaag
     9421 tgcggcgcca cgcatgaccg gcaagggttc caggcggcgc atgacgccgt tgcggcgcct
     9481 cgcgtaccgc atcatcgtgc cgatcggcgc cgcgctgatc cgcctggtgt ggggcgtcgc
     9541 gcggcgcccg cgcgtcgtgg gtggagagca tctcgatgcg gcgctggcga tcgcgccgtc
     9601 gttcatcccc gtgctgtggc accagcacca gcttttctgt gccaagtggc tgctcgagca
     9661 gcgcccccgg gtcaccctgg gtttcctgat cagcccctcg gtcgacggcg agattggtgc
     9721 gatgctggtc gatcgtttcg gaggtgtggc ggtgcgcggc agctccaccc acaccggcgc
     9781 gcgcgcgctg cgcgactact atgaggcgct cgtcaagcgc gatgtctcgc cggtcatcac
     9841 gcccgatggt ccgcgcgggc cgcgcttcgt gttcaagccc ggcgcgatcc tgctcgcgca
     9901 gatgtccggg cggccaatgc tgccgatggc ctatgccgca agccgggcca tcctgttcca
     9961 ctgggaccgg ttcgtgattc cgctgccgtt ctcgcacatc gtgatcgccg tcggcgctcc
    10021 gcggtgcgta ccgcgcaccc tcggcgcggc cggcatgcag cagtggcagg aggacatgac
    10081 cgcggagctg aagtcgctgt acgcgcgcgc gcatgcggcc ctgccggtcc gcgattgagc
    10141 tgcgggcgcg gcgccaccgc ggcgcgatgg ggctgtctcc tgcccgaata tgtcgaatcg
    10201 ctcggtggcg aaaataccgt aatcatcagg cggcacatgc gcttagcttg tattcctcat
    10261 tggctgtgac acaatccggc ggtaactgac tcatgtgccg tgcgcgcgaa gtgtgtggca
    10321 cgtcatcggc aggggggcac acggtttgtc agaatgcgag ctcgcaatct tggggatcga
    10381 acctcggtgc ccggcgtaac ttcagacaaa cccagcgaat tcacggcatc cgacatcctc
    10441 gctcccggcg tatggaaggt caggggtgcc cgtcccgcac tcgggatcga cgtcgtgtgc
    10501 accaagatcg cggtcgatct gcagaacatg cagcgcgaga cgctggcggc ggtcctggcg
    10561 cgcagcctcg agtcgctgcg cgaggctacc gcctgtgatg cggcgttcgt cgcgatgttc
    10621 aatgacgagg ccgtcaccga actacacgta gcgcgcggca ccttcgcgca gacccgcctc
    10681 gaggaactgc gtggcgtcgc gctggaatcc ctcccctggc tcaagtcgcg cctggaacac
    10741 ctgcgcctct ccgagttgcg tgacaccgcg gcgccgagga aggagaacgc ggtcgagtcg
    10801 cgcctcttcg cttcgctgaa ggtcggttcg accctctatg tcgccttccg cgctctgggc
    10861 cgccccgcgg gcatcctggg ccttgccaac gcgatgtcgc gcggcccgtg ggacgtgaat
    10921 gtccagctgc tcctgaagct cctcggcacc agcctggcta ccggtctcga tcgcgtgcgc
    10981 ctgcgcaccc gcctcgacga cctcgaagtg cgcgtcggcc tggcgcaggc cgcggcgaac
    11041 gagggcatgt gggacttcga caccgagacc aacgtggtga gcttctcgcc gcgctggaag
    11101 gagatgctcg gttacagcga cgaggacctg tcgggcccga ccgactggcg tgcgctcgtg
    11161 cacccggacg acatgtcgcg cgtgcagacg gcgatgcgcg agcacgtcga gggccatgcg
    11221 ccgatctgcg agagcatcca ccggatgcgc catcgcagcg gcgagtggcg ctgggtggtg
    11281 agccgcgcca aggcgcacgt cgacgagaac ggccgcctgt tgcgcctcgt cggcatggaa
    11341 ttcgacatca ccgagcgcaa gctctacgaa gaggcgctgt ttcgcgaaaa ggaaagcgcg
    11401 cagatcacgc tgcagtcgat cggcgacggc gtgatcacca ccgacgccaa ctcgatcatc
    11461 gactacatca acccgatcgc cgaggaactg accggctggc gcctcgagga tgccatgggc
    11521 cgctcggtcg acgatgtctt caaggccttc cacgaggaga cctgcgagcc gctcgagaat
    11581 ccgctgacga tgtcggtgcg gcgcatccgc ccgatcaagt cggtgcggcc gatgctgctg
    11641 atccgccgtg acggcaacga gctgtacgtg gaaagcaccg cagccccgat ccgcgacggc
    11701 gccggcaagg tctgcggtgg cgtactggtg ttccacgacg tctccgagtc gcgcgaactg
    11761 aaccggcggc tgtcctacca cgcgagccac gacatcctga ccggcctcgt caaccggcgc
    11821 gagttcgaga accggctcga gcgcgcgctg aagagcgcca gggcccgcga ggcctcctac
    11881 gcgctgtgct acctcgacct cgaccagttc aagatcataa acgacacctg cggccacagc
    11941 gccggcgacg cgctgctcgg tcaggtcggc gcgctgctga agtcgaaggt gcgctggcgg
    12001 gatacgctgt cgcgcctcgg cggcgacgag ttcggcatcc tgctcgagag ctgctcgctc
    12061 gatgaggcga tgcgcacggc cgagacgctg cgcgaggcgc tgcgcaattt ccgcttcacg
    12121 tgggaagacc gcgtgttccg cctcggcgcc agcatcggcg tcgtgccgat cacggccgac
    12181 aacgaggacg tcgcctcggt gctgtcggcc gccgacagcg cctgcgccgc cgccaaggaa
    12241 gcgggccgca accgcgtgca cagcttcgcc gagaacgaca tcgagctgat gcgccgccgc
    12301 cgcgaaatgc agtgggccgc gcgcatcaac tcggcgctgg aagagggccg cttcgagctc
    12361 taccggatga cgatccagcc gctgcaaccc tcgagcctga tccagggcgc gcactacgag
    12421 ctgctgctgc gcatgcgcga cgagaacggc cgcatggttt cacccgacaa cttcatcgcc
    12481 gcggccgaac gctacggcat cacgccgaac atcgaccgct gggtgatcga gaacgcgctg
    12541 cgctggctgg tctcggaggc cgacgagcgc gagcgtctcg cgatgtgctc gatcaacctc
    12601 tcgggccaga gcttcagcga cgacaaattc ctgcctttcg tgatcgacca gttcgcgaga
    12661 agcggcatcg acgcctcgaa gatctgcttc gagatcaccg aaacggcggc ggtcgcgagc
    12721 ttctcgcagg cgaaccgctt catccaggcg ctgaaggagc tcggctgcaa gttcgcgctc
    12781 gacgacttcg gcaccggact gtcatcgttc ggttacctga agcacttccc ggtcgatttc
    12841 ctgaagatcg acgggagttt cgtgcgcgag atcttgcgcg acccgatcga ccgcgagatg
    12901 gtgcgttcga tcaatgaaat cggccatctc accggcaagc agaccattgc cgagttcgcc
    12961 gagaatgcgg agatcatcca gatgctgacc ggcctgggag tcgactacgc gcagggctac
    13021 ggcatcgccc agccgcaacg cgtgctgaag gccgtcaacg cctgatcggt gtccgggcgg
    13081 attcgctccc gccggcgcgc tatgctgtgc accggtcggc atggggcggg ggataccacc
    13141 atgggattca atcggaccgg actgctctcg gcagcggcgc tgtgcatggc cgcgatacct
    13201 gcgccagccg cggaccgcta cgacatcatc ttcgccggca acctctccgg ccagcagacg
    13261 acggccgttg ccgccgacgg cagtgtcggc agcacgctgt cctggcgtaa caacgggcgt
    13321 gggccggata tcgaggagcg catcgtcgtt gcggcggacg gcaccctcgc gagctatcgc
    13381 gcgaccggta cctcgacctt cggctcgaag atcgacgaga ccttcagccg tcgcggcgat
    13441 cgggtcgtgt ggcgctcctc cgccgaccgg ggcgaccgcc gggtcgacgg tccggcgctg
    13501 tacctgccgg tggagggcag cttcgaggcc accgccgtga tcgcgcgcgc gctgctcgcc
    13561 gctcccgggg gcaggctcgc cgcgctgccg ggcggcgaac tgtcgatcga gcggctcgcg
    13621 gccacggcgc tcgagcaggg cggcgaacgg cgggccgtgg cgctctatgc gatccacggc
    13681 atgtccacgc agccgcgttt cctctggctg agcgacgatg acgagcggcg tttcttcgca
    13741 ctcgtctacc cgggatacat gctggtggcg cccagcggct ggtcacaggt cgccgcggcg
    13801 ctcgaggcgc tgcagaagga tgcggagcag aagctcctcg aagcgctcgc cgcgcggctt
    13861 gcgcatcgcc tcggtgacga gctcgtgatc cgcaatgcac gcgtgttcga cagcgacacg
    13921 ggtgcgctca ccgcgccgca ggacgtctac gtgcacgacg gacgcatcgc cgcgctctac
    13981 cccgcgggat cgcggccgag tggcgccatg cccgcgatcg acgcggacgg ccgtgcgctg
    14041 ctgccgggcc tgttcgacat gcacgtgcac gatgacgcct ggaacggcct gctgcatctc
    14101 gccggcggcg tgaccacggt tcgcgacatg ggcaacgaca accgcctgct gcgcgaactc
    14161 gccgtgaagg gcgaggcggg acgcctcgcg gcgccgcgca tcatcgccgc cgggttcatc
    14221 gagggtgaaa gtccgcacgc cgcgcgcgcc gatttcgtcg tcggcgacct cgaggcggcg
    14281 aaggccgccg tcgactggta tgcacagcgc ggttacctgc aggtgaagct ctacaactcg
    14341 atcaggccgg agtggatggc gccgatcgcg cagtatgcgc acgagcgcgg catgcgggtc
    14401 agcggccacg tgcccgcgtt cgcgcgcgcg gaggacgccg tgcgcgccgg ttacgacgag
    14461 ctgcagcaca tcaaccaggt ggtgctcaat ttcctcgtca tgcccgacac cgacacgcgc
    14521 acgctggcgc gtttctacct gatcggcgag caggccgccg acatcgacct cgactcgcgc
    14581 gaggtgcgcg acttcatcgc gctgctcagg gagcgcggca ccgtgctcga cccgaccctc
    14641 gcgacattcg agtacatgtt ccagcaggcg cgggggcagc cgaatccgac cttcgcggca
    14701 gtggccgacc acgtgccgcc cgcgctcgcg cgcgagtggg gcaccaacgt gatggacatc
    14761 ccgcccgcca aggtcacgcg ctaccactcc ggctacgaga agatgctcgg cttcctgcgc
    14821 ctcgcccacg aggccgggat cacgatcctc gccgggaccg acgacatcgg cggtttcacg
    14881 ctgcaccgcg agctcgagct ccaggtcaag gcgggcattg cgcccgccga tgcgctcagg
    14941 atcgcgaccc gcaacggcgc gcgggtgctg ggactgtggg acgacagcgg ctcgatcgag
    15001 cgcggcaagc gcgccgacct gatcctggtg gaaggcgacc cgacgcggga catttcggcc
    15061 atccgccgta tcggcctcgt gatgaaggcc ggggtcgcct acttcccggc tgaaatccac
    15121 gaggcgctcg gcgtgcggcg tttcgccgat ccgccgaaag tgaccctgcc ctgaccggcg
    15181 tgcctagccg cgcgtgatgc gcagcgacag gtcgagcgcg tgcacatgct tggtgatcgc
    15241 gccgaccgag atgtagtcca caccggtctc ggccacggcg cggatcgtcg cgagcgacac
    15301 gttgccggag gcttcgagct tcacgggccg gccgagctcg cgattgcgtg ccaccgcagc
    15361 gtgcatgccg tcgaggctga actcgtcgag catcaccacg tccgggcgtg cggccagggc
    15421 atgctcgagt tcggcggcgt tttcgacttc gacttcgatc ggaatgcccg gcgagagccg
    15481 gcgtgccgtg gcgatcgcca ccgggatcga cccggcggcc gcgatgtggt tttccttgat
    15541 cagcaccatg tcgtgcaggc ctaggcggtg gttctgcgcg ccgccgcagc gtaccgcgta
    15601 tttctgcgcg aggcgcaggc ccggcaccgt cttgcgcgta tcgaggatcg tgcagccggt
    15661 gccggccacg gcgtcgacat agcggcgcgc ggcgctcgcg gtgccggaca gcgtctgcag
    15721 gaagttgagc gcggtgcgct cgccggtcag cagcggccgt gcggggccct cgatccggca
    15781 cagcacggtg tcgcgcccga cgcgctcgcc atcggcggcg taccagtcga tgcgcagccg
    15841 cggatcgagc gcgcggaaca cggcatcgaa ccaggccgtg ccgcacagga tggcggcctc
    15901 gcgcgccacc acctgcgcct gcgccagctc gcgctcgggt acgagcgcgg cggtgacgtc
    15961 gccgccgccc acgtcttcgc gcagtgcggc ggcgacctgt tcctccaggt tgtgcggcag
    16021 ctcggtcaga acggcagacc gtgcgtggcg ccggccatgc acatcagcaa cgggaacgag
    16081 aggatgaagt tgacgcgcga ggtgttgccc gcgatcacac gggcggcagc cttctgctcg
    16141 tcggtcgcgg cgacgatgcc gagcaccttc ttctggttcg gccagatcag cacccagacg
    16201 ttgaacagca tgatcgtgcc gagccacgcg ccgatgccga tgacgaggag gtagtagtcc
    16261 accggattgc tggtcaggcc cagcgagaac gcgccgggga atctcgcacc caggtaccac
    16321 gcgcccgaca gccaggtcac gagcgccgac cagcggaacc agaagagtgc gcgcggcgcc
    16381 acgtacttcg agatcccggc gccgcccggg ccgcccttgt cggccgtggc ggcggcgagc
    16441 gcaggggtct gcacggcgtt gaagtaatag agcaggccga tccagaacac gccggcgacg
    16501 atgtgcaccc agcgcgcgag gccgagattg tgataggcga tcgggtctga aaccacggcg
    16561 ttgatgatgt aggccaggat cagcgcaagg accacgccgg tgaccagggc gtatcggtga
    16621 ttggacagga ttttcatctc gcgggctccc caggttattg ttttggcagg gctttggacc
    16681 gtgtctgaat tgaagaatag gcgcgggggc cccataattc aatgttgcac ccccgcgcct
    16741 gagaggactc gaatggacgt cgttgatcgc atcaagcaac acctggccgc ccccgtcgta
    16801 ctcttcatga agggcacgcc cgacttcccg cagtgcggat tttccgctca ggtcgccgct
    16861 gcgttgcgcg cggtgggcgc gaagtacgag gccgtgaaca ttttcgagga tctcgagttg
    16921 cgcgacgagc tgaagcgcta ctccaactgg ccgacctacc cgcagctgta tgtcaacggt
    16981 gagttgatcg gcggctgcga catcgtgctc gacctctaca ggagcggcga gctcgccaat
    17041 atcctggccg ccgccggcaa ggccgcctag gagtctgggc ggcagaacct actgacgagt
    17101 ggagtgaggt gaggtagt
//
LOCUS       DAOQVY010000046        16801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_3892_length_16801_cov_6.085155, whole genome shotgun sequence.
ACCESSION   DAOQVY010000046 DAOQVY010000000
VERSION     DAOQVY010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16801
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3892_length_16801_cov_6.085155"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..587)
                     /locus_tag="PKN91_06110"
     CDS             complement(96..587)
                     /locus_tag="PKN91_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNS27681.1"
                     /translation="MIRFETTLGSFDIELNEKDAPISSANFLAYVDEGFFDGTLFHRV
                     IPGFMIQGGGMTSGLEQKATRAPIENEAKNGLKNLRGTLSMARTNDINSATSQFFVNL
                     VDNDFLNNAPGNYGYAVFGRVIAGMDTIDKIAQVRTGRSRGHSDVPVEEVVVKSARRI
                     DAA"
     gene            complement(584..1459)
                     /gene="prmC"
                     /locus_tag="PKN91_06115"
     CDS             complement(584..1459)
                     /gene="prmC"
                     /locus_tag="PKN91_06115"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNS27682.1"
                     /translation="MGETLADEGVTLALLVDRAAAALGGVDARREAEALVAAALHIGR
                     ATLLAHPERRIAGEALARVQDWIGRRARGEPLAYLAGEREFWSLPIRVTPAVLVPRPE
                     TEELVACALALGGDGERAIVDLGTGSGCIALAIAHERPHWRVTATDASRAALGVARAN
                     AAALGLDHIEFLHGDWYAPLTGRHFDLVVSNPPYVAEDDAALDDLALRHEPRGALTPG
                     GDGLAALRQIIDGAPRVLRPGGWIVLEHGADQARDVAALLVARGFTHVRCAPDLAGHE
                     RVTSGGLPSNTTGLP"
     gene            1458..2288
                     /locus_tag="PKN91_06120"
     CDS             1458..2288
                     /locus_tag="PKN91_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27683.1"
                     /translation="MPTPILPFAAPPSARDVIEASLRLFGRSVLPCLPLATIGTLIGQ
                     LPTAYDVAVHGKPMPINAETLADKDGQWWALYLIGILASLVVWALVLLKQQRIALGTA
                     HTVPLGPTLARLPAILAYLVASFFVVLLATLPFGLIVGLVPLPQALGTAIVMLPLLAT
                     SVAVSLGWPALVVEGRGPFAAIDLGLRLTAAHWKRMLVVLIAIVATVIVIVIIASLTV
                     GVLAGLASVAGESVQNSVAVAIVIATVALLVLYLSAYLLVVFNDLRQRAQVSSSSSEA
                     "
     gene            complement(2258..3337)
                     /gene="prfA"
                     /locus_tag="PKN91_06125"
     CDS             complement(2258..3337)
                     /gene="prfA"
                     /locus_tag="PKN91_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNS27684.1"
                     /translation="MKDSIRQRLERSLDRTEEVGRLLADPDTAGHSREFRELSMEYSR
                     LEPLAARYRLYRGLELELAAAREMTGDPDPELRELGAEEARRLEAALAEEERALRELL
                     APQDPHDERNIFLEIRAGTGGDEAAIFAGDLLRMYTRYAERRGWAVELISQSPGEHGG
                     YREVICRINGKGAYSWFRFEAGTHRVQRVPATEAQGRIHTSAVTVAVLPELDEVDAIA
                     VNPADLRIDTFRSSGAGGQHVNKTDSAIRITHLPSGIVVECQDERSQHKNRSRAMALL
                     SARLLAAETEKRNAAEAAARKSQVGSGDRSERIRTYNFPQGRVTDHRVNVTLYRLAEV
                     MEGDLDGLIEPLRQEYQASLLDEET"
     gene            3389..5047
                     /locus_tag="PKN91_06130"
     CDS             3389..5047
                     /locus_tag="PKN91_06130"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS27685.1"
                     /translation="MRSSILLMAVTAIAACAAEPQITTPADADAAAIVAEVALERGDC
                     RTAAEQYAKAATVRGAPVATLQRAVEVAMDCQQFETAGKLAPLWRARAPDSAAAARQA
                     GLVALRLGRNSGAAAAFRDAIRLAGEEPSKALVDLAQASAGTGNDFGAYAALHAAADP
                     GRLDASALVVLGNLALESFVFRDARALAEAAIARDPQSAPAQLLLSRVLAGTDDAAGA
                     IAAANAARRIDPAGQAFALAEILVALDRIEEARMELERLLADDDASPEAERRLALLAF
                     REGDTQEAAGRFSQRLRTGDGAGEALFYLGALAERHGNDPVALEMYRQLVQAGAGMLA
                     RRRAAFVLMKMGKREEAFSLLDAEVATEPGTAIDVVLAKSSLLIGADDAAAAVDLLDA
                     ALDLYPGHPGIEYQRALALENAGRVPESIAAFETMLKRRPEDPVTMNALGYTLADNDR
                     SLARANALIDDALALTPDNPATLDSQGWVRFRRGQGEQALESLARAYRLSRDTEIAAH
                     WGEVLWSLGRESEARTIWARALARDPGSTLLKDTIGRFIPPAAP"
     gene            5044..5655
                     /gene="lolB"
                     /locus_tag="PKN91_06135"
     CDS             5044..5655
                     /gene="lolB"
                     /locus_tag="PKN91_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HNS27686.1"
                     /translation="MTRRLRRHFARTAFVAALGALLAACVSAPVRDSSAVPWPERRAA
                     LQEQTEFGLTGRVAVNSAGAGFNASLRWQQQGGLSQVSLDGPLGVGGAQIEVADGHFD
                     LRTSRGEHLEGDAARAALEERLGFPLPLAELRYWMRGVPAPGAAADEVIDEARQRLTR
                     LVQQGWEIDYGEYLEGAGGSLPRRVIAQRGGTRVRVVVDRWTP"
     gene            5652..6524
                     /gene="ispE"
                     /locus_tag="PKN91_06140"
     CDS             5652..6524
                     /gene="ispE"
                     /locus_tag="PKN91_06140"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNS27687.1"
                     /translation="MSAPHAQRERWPAPAKLNLFLHITGRRADGYHTLQTAFQLIDLA
                     DSIEIEATSDCEIRRLEGPDGVPAEADLAVRAARALQVATTVRRGARLRIEKRIPVGA
                     GLGGGSSDAATTLVALNELWGTGLDLRQLASIGADLGADVPLFIHGRSAFAEGIGDLL
                     MPLELPEKWFLVVYPGVAVATAAVFQSPELTRNSPLITIRDLFGAETHNDCEPVVRAR
                     FPAVGEALDWLGQFAGARLTGTGSCVFASFDSASAAGQVAAKVPQSWRSYVARGLNHS
                     PLHAQLARLRGESE"
     gene            6541..6615
                     /locus_tag="PKN91_06145"
     tRNA            6541..6615
                     /locus_tag="PKN91_06145"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6573..6575,aa:Gln,seq:ttg)
     gene            6653..7591
                     /locus_tag="PKN91_06150"
     CDS             6653..7591
                     /locus_tag="PKN91_06150"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNS27688.1"
                     /translation="MALFAGNANRALAQDIARNLKQPLGRAYVGKFSDGEVNVELIEN
                     VRGRDVYLLQPTCAPVNDNLMELLVMADACRRASAARVTAVVPYFGYARQDRRPRATR
                     SAITAKLVANMISGAGVDRLLTVDLHADQIQGFFDIPVDNVYGSPVLLGDAWRQGYED
                     MIVVSPDVGGVVRARALAKRLDDADLAIIDKRRPRPNESKVMNIIGEVADKTCVLVDD
                     MVDTAGTLCLAAQALKDQGAKRVVAYITHAVLSGSAVQKISGSALDELVVTDTIPLTE
                     AARDCGRIRQLSVAGLLAETIRRIRDEESVSSLYID"
     gene            7653..8330
                     /locus_tag="PKN91_06155"
     CDS             7653..8330
                     /locus_tag="PKN91_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNS27689.1"
                     /translation="MRIGFEIQADFRDDQGKGASRRLRRHGKIPAILYGGKLAPRQIA
                     LDHQHMLTLVDNEKLYSSIIRLKVGDETQEAIIKDVQMHPAKNAIVHIDMLRVLADEK
                     IRIYVPIHFKGAAAAPGVKTQGGVVSHRISDVEVICLPKDFPEFLEVDMSEMQLNETK
                     FLSDIPLPEGVVIPQLQHGRNLAVVSVHAPRAAEPEPTEAAAAAEGAVAAAPAAEAPA
                     KEGGGKK"
     gene            8415..9002
                     /gene="pth"
                     /locus_tag="PKN91_06160"
     CDS             8415..9002
                     /gene="pth"
                     /locus_tag="PKN91_06160"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNS27690.1"
                     /translation="MAEFPLKLIVGLGNPGDEYARTRHNAGFWFVEELAARYGGTMRS
                     DRHHRGETARVRIGDAEVLLLKPMQYMNRSGAPVGSIVDFYKIDVSSILVAHDELDLP
                     VGTVRLKEGGGHGGHNGLRDLIPRIGEGFWRLRIGIGHPGDKSEVVDYVLTRAPAGED
                     ATIRAAVAAAAEVVPLLLAEGPQKAMHRLHTNVKD"
     gene            9011..10102
                     /gene="ychF"
                     /locus_tag="PKN91_06165"
     CDS             9011..10102
                     /gene="ychF"
                     /locus_tag="PKN91_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNS27691.1"
                     /translation="MAIKCGIVGLPNVGKSTLFNALTRAQIAAENYPFCTIDPNVGVV
                     PVPDPRLDELAAIQKPQKVLPTTVEFVDIAGLVAGASQGEGLGNQFLAHIREVDAIAH
                     VVRCFENDDIVHVAGKVDPLADIETIDTELALADLQSVEKALDRAAKAAKAGDKEALR
                     KRDLYERVRGQLDAAKPVRAMGLGADELRDLQDLHLLTAKPVMYVANVDEGGFTDNPR
                     LDALRARAASEGAQVVAVCAAIEAEIAQLDDDARAEFIADLGLDEPGLNRVIRAAYTL
                     LGLQTYFTVGPKEVRAWTVRRGATAPQAAAVIYTDFERGFIRAEVVGYDDFIACKGEA
                     GAKEAGKLRLEGKEYIVREGDVMHFRFNV"
     gene            10221..11099
                     /locus_tag="PKN91_06170"
     CDS             10221..11099
                     /locus_tag="PKN91_06170"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNS27692.1"
                     /translation="MCDHDSFDDMVEYQLRSKGLSRRQFSALTLGAGALSVFPPLAGA
                     AEVVESEVEIETPDGTADACFAHPAQGAHPAVLVWPDIFGLRPAFRQMGRRLAESGYA
                     VLTVNPFYRTQKAPTAPENPDFNDPATRTSLMTLRATLSAATGATDARAFVTWLDAQP
                     AVDKRRRMGTTGYCMGGPLTMYTAAERSDRIGAGASFHGGGLATDKPDSPHLLVPDMK
                     AQFLIAIAENDDQRDPEGKDTLRKAFAAAGLPAEIEVYAGTKHGWCPPDAAVYDHDQA
                     EKAWARLLALFERALA"
     gene            11183..11923
                     /locus_tag="PKN91_06175"
     CDS             11183..11923
                     /locus_tag="PKN91_06175"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNS27693.1"
                     /translation="MKIRDLPPLRRRDVLKAAGAVAVFGTLPFAGRIAAADTPKPKIG
                     MIGSGRVGSALGRVWAHAGHEVMFSSRNLEADRELAAAVGANARAGTSAEAAAFGEVL
                     VFAVPYGALPELGQALAEQIRGKVVIDACNPFPNRDGEIANRALERGAGVVSAELLAG
                     ARVVRAFNAINARRMGSAHEEPGRIGMPIAGDDEEAIRIASILIREIGYEPVLVGGSG
                     MGKYLMPGTPLAGEHTAAEIRQIAATLR"
     gene            complement(11920..13836)
                     /locus_tag="PKN91_06180"
     CDS             complement(11920..13836)
                     /locus_tag="PKN91_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27694.1"
                     /translation="MPPLKRISPFLAPLLLLALNSCGGGGGGSTPAPTAGGPATGSCG
                     APDCGMAMITITDADGDFLAYSVDVVSLTLTRANGQVVQTLPVTSRIDFSQLVGVSEF
                     VTAATVPQGAYVAGTIRLDYTNADIQVEVAGQPVQATVIDEDGEPVGVIDVEVRLDNR
                     NHLVVSNGRPALLQLDFDLAVSNSVDLEADPVTVTPAPIIVATVDRPDSRELRVRGPL
                     VSVDIDAGTYEVDVRPFSHKSAKLGRVTVHTTADTAWELDGQAYVGADGLAALATLPE
                     GTLTAAFGTLSKRDHVFTADRVLAGSSVEDDDLDFAVGNVVARAGDTLMLSGGRMSRH
                     DKPDWLIMRHPVTVLVGPETSVTRAGETGDVDIGAISVGQRVKVFGVASRGDDSDEAQ
                     VADGSERQIDDDDVVTIDATAGRVRLDVTQLAGTVTGVAGTTLTLDLDSIDRWHVERF
                     DFTGTGTPGQDADPAAYEVDAGALDLARFDVGSPARLFGFVAPFGAAPPDFTARTVVD
                     FEDVMAKLAIGYWPRGTAAPFTSLDANAIVIDPANSAISWPHWIKTGTTIHDVEKLAT
                     PLRIVPTAEATGTFAIGDGHEVDNFETFADFTAALADELDGTSRVVGLGAEGDFDAGT
                     GDFTVTRMLVRMKR"
     gene            13988..14064
                     /locus_tag="PKN91_06185"
     tRNA            13988..14064
                     /locus_tag="PKN91_06185"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14022..14024,aa:Met,seq:cat)
     gene            complement(14077..15177)
                     /locus_tag="PKN91_06190"
     CDS             complement(14077..15177)
                     /locus_tag="PKN91_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27695.1"
                     /translation="MWNRRELMKLGLGAASLAAVHRSVAAQAAPLRLLILGGTGFLGP
                     HLTEQALQRGWAVTHFNRGKRDADGVPGVETLHGDRKGQLDALRGHAWDAVIDNTGYI
                     PKYVKMSAELLAPNAGYHLFVSSISAYASFAKPNDESSPTGHLANPEEEAVTNESYGP
                     MKALCEQYSMQAFEGRASVVRPGYIVGPLDPTDRFTYWPVRAARGGEMLAPGTPQDPI
                     QIIDARDLTAWMLALVERRVTGYFNAVTRPREYTIGDIVRASIAASPQAGTKATWVPE
                     DFLFKQWQPEQVDLPPWSPMGGEYAGASLTASDRAQAAGLRTRPLEDTVRDTLAWHRT
                     RPAERQEKLRAGLDPELERTTLAAWHARGKNG"
     gene            complement(15191..16492)
                     /locus_tag="PKN91_06195"
     CDS             complement(15191..16492)
                     /locus_tag="PKN91_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNS27696.1"
                     /translation="MKRIAMLAVLLGCASGAAAQATFHGDNARTGVYAGSGPAQLQGV
                     KWSFDTGGPVLASPVVAGDLVYIASASGALLAIDRHSGRELWKFVSRMPIASTPAVEG
                     DTLYFVSSAGSLVALDAATGQLRWIFATEHERHFEARGLHGYGPAGQTIADAWDVYTS
                     SPAVADGKVYFGSGDGNVYAVDARSGVLQWKFPMRGVVHASPTVVDGTVYVGSFASDF
                     HALDAATGQSKWIFQAGEDPANHNQVGFQSSAAVVDGVVYVGCRDAHVYALDAKTGRK
                     RWDYPTSKSWVNGTPAVRNGIVYVGTSDTSRLMAIDAKSGRLRFNFDAKGFVFSSAAL
                     AGGRVYFGSHNGRLYALDADDGRLAWTFQTEASKQDPLKLLKPDGSFDRDGFAPVFRD
                     FRDMYIDLFRFRSIGGILSSPTVADDVIYVGSLDGNLYALH"
     gene            complement(16575..>16801)
                     /locus_tag="PKN91_06200"
     CDS             complement(16575..>16801)
                     /locus_tag="PKN91_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27697.1"
                     /translation="RIVPPDGAFYLYADVGHLTGDSMQFCLRAVDEIGVGLAPGIDFD
                     PDAGRHFVRFSFAVTPEEIERALELLAEWL"
BASE COUNT         2607 a         5577 c         5931 g         2686 t
ORIGIN      
        1 gcgcggacag cgcgagacca agcaccaaga gcccgagcat cgcgcgcacg aggcacaggc
       61 cgaagctcct gcggcgagcc aagagcgcgt gccgctcagg cggcgtcgat gcgccgcgcc
      121 gacttcacga cgacttcctc gacgggcacg tcgctgtggc cacgcgagcg cccggtgcgc
      181 acctgcgcga tcttgtcgat cgtgtccatg cccgcaatga cgcggccgaa taccgcatag
      241 ccgtaattgc cgggcgcatt attgaggaag tcgttgtcga ccagattcac gaagaactgg
      301 gaggtcgcgc tgttgatgtc gttggtgcgg gccatcgaca gcgtgccgcg caggttcttc
      361 aggccgttct tcgcctcgtt ctcgatcggc gcgcgggtcg ccttctgctc gaggccggag
      421 gtcatgccgc cgccctggat catgaagccc ggtatcacgc ggtggaacag cgtgccgtcg
      481 aagaagccct cgtcgacgta ggcgaggaaa ttggccgagg agatcggcgc gtccttttca
      541 ttcaattcga tgtcgaagct gccgagggtg gtttcgaagc ggatcatggg agtcctgtgg
      601 tgttggaggg caagcccccg ctggtcacgc gctcgtggcc ggcgagatcg ggggcgcagc
      661 gtacgtgagt gaagccgcgc gcgacaagca gagcggcgac atcccgggcc tggtcggcgc
      721 cgtgttcgag cacgatccag ccgcccggcc gcagcacgcg cggcgcgcca tcgatgatct
      781 gccgcagtgc cgcgagaccg tcgccaccgg gcgtcagggc gccgcgcggt tcatgacgca
      841 gcgccaggtc gtcgagcgcg gcatcgtcct cggcgacata cggcgggttg ctgacgacga
      901 gatcgaagtg ccgcccggtg agcggggcgt accagtcgcc gtgcagaaac tcgatgtgat
      961 cgagcccgag cgctgcggcg ttggcgcgcg cgacgccgag tgcggcgcgg gatgcgtcgg
     1021 tggccgtgac gcgccagtgc gggcgctcgt gcgcgattgc aagcgcgata cagccgctgc
     1081 cggtgccgag gtcgacgatg gcgcgctcgc cgtcgccgcc aagtgcgagc gcgcaggcca
     1141 cgagctcctc ggtttccggg cggggcacga gcaccgcggg cgtgacccgg atcggcagcg
     1201 accagaactc gcgctcgccc gcgagatagg cgagcggctc accgcgcgcc cgccgcccga
     1261 tccagtcctg cacgcgcgcc agcgcttcgc cggcgatgcg ccgctcggga tgcgcgagca
     1321 gcgtggcgcg gccgatgtgc agcgcggcgg caaccagcgc ttccgcttcg cggcgggcat
     1381 ccacgccacc gagcgccgcg gccgccctgt ccaccagtag cgcgagtgtc acgccttcgt
     1441 ctgctagagt ctcacccatg ccaacgccca ttctgccatt tgccgcgcca ccgtccgcgc
     1501 gtgatgtgat cgaggccagc ctgcgcctgt tcggacgctc ggtgctgccg tgcctgccgc
     1561 ttgcgacgat cggcacgctg atcggccagc tgccaactgc ctacgacgtg gccgtgcacg
     1621 gcaagccgat gccgatcaac gccgagacgc tcgcggacaa ggacgggcaa tggtgggcgc
     1681 tgtacctgat cgggatcctc gcgagtctcg tcgtctgggc gctcgtgctg ctgaagcagc
     1741 agcgcattgc gctcgggacg gcgcataccg tgccgctcgg ccccacactc gcccggctgc
     1801 cggccatcct cgcctatctc gtcgcaagtt ttttcgtggt gctgctcgcc accttgccct
     1861 tcgggctgat tgtgggtctt gtgccgctgc cgcaggccct gggcacggca atcgtgatgc
     1921 tgcccttgct ggcgacgagc gttgcggtga gcctcggctg gccggcgctc gtggttgagg
     1981 ggcgagggcc gttcgccgca atcgacctcg ggctcaggct cacagcggcg cactggaagc
     2041 gcatgctggt cgtgctgatc gcgatcgtgg cgacggtcat cgtgatcgtg atcatcgcga
     2101 gcctgacggt gggcgtgctg gcggggcttg cgagtgtggc cggcgagtcc gtgcagaaca
     2161 gcgtcgcggt ggccatcgtg atcgccacgg tagcgctgct ggtgctctat ctcagcgcct
     2221 acctgctggt cgtgttcaac gacctgcgcc agcgtgctca ggtctcctcg tcgagcagcg
     2281 aggcctgata ctcctgccgt agcggttcga tcaggccgtc gaggtcgccc tccatcacct
     2341 cggcaagccg gtagagcgtg acattcaccc gatggtcggt gacccgcccc tgcggaaaat
     2401 tgtaggtgcg gattcgctcc gagcgatcgc cgctgccgac ctggctcttg cgtgcggcgg
     2461 cttccgccgc attgcgcttc tcggtctccg ccgccagcag ccgtgccgac aggagcgcca
     2521 tggcgcgcga gcggttcttg tgctgcgagc gttcgtcctg gcactcgacg acgatcccgg
     2581 agggcaggtg ggtgatgcgg atcgccgagt cggtcttgtt gacatgctga cctcccgcac
     2641 ccgatgaacg gaaggtatcg atgcgcagat ccgccgggtt cacggcgatc gcatcgacct
     2701 cgtcgagctc gggcagcacg gcgaccgtga cggcggaggt atggatgcgg ccctgcgcct
     2761 cggttgccgg cacgcgttgc acgcgatggg tgcccgcctc gaagcggaac cacgaatacg
     2821 cgcccttgcc gttgatgcgg cagatcactt cccggtagcc gccgtgctcg ccggggctct
     2881 gcgagatgag ctccacggcc cagccgcgcc gctccgcata gcgcgtgtac atgcgcagca
     2941 ggtcgccggc gaaaatcgcg gcttcatccc cgccggtgcc ggcccggatt tcgaggaata
     3001 tgttgcgttc gtcgtgcgga tcctgcggcg cgagcaactc gcgcagcgcc cgctcctcct
     3061 cggccagcgc cgcttcgagt cgccgggctt cctccgcgcc gagttcgcgc agctccgggt
     3121 ccgggtcgcc ggtcatctcg cgcgccgcgg cgagctcgag ctcgaggccg cgatacagcc
     3181 gatagcgcgc cgcgagcggt tcgaggcgcg agtattccat cgacagttcc cgaaactcgc
     3241 gcgaatggcc agccgtgtcg ggatcggcca gtagccgccc cacttcttcg gtgcgatcca
     3301 gggaacgctc gagccgctga cgtatcgaat ctttcatgga atcgcggaat tctccgggat
     3361 tggagcgcct tccgctatag tgacgagaat gcgaagcagt atcctgttga tggccgtgac
     3421 cgcgatcgcg gcgtgtgccg ccgagccgca gatcaccacc ccggccgatg ccgatgccgc
     3481 cgcgatcgtc gccgaagtcg cgctcgaacg tggcgattgc cgcaccgctg ccgagcagta
     3541 tgcgaaggcc gccacggtgc gcggcgcgcc ggtcgcaacg ctgcagcggg cggtcgaagt
     3601 ggcgatggat tgccagcagt tcgagaccgc cggaaagctc gcgccgctgt ggcgcgcgcg
     3661 cgcacccgac agcgccgcgg ccgcacgcca ggctgggctc gtcgcgctgc gtctcggccg
     3721 caattcgggt gccgcggcgg ccttccgcga cgcgatccgg ctcgcgggcg aagagccctc
     3781 caaggcactc gtcgacctcg cgcaggcgag cgcgggcacg gggaatgatt tcggtgccta
     3841 tgcagcgctg cacgccgccg ccgatcctgg caggctcgat gcatcggcgc tcgtcgtgct
     3901 cggcaatctt gcgctcgaga gcttcgtgtt ccgcgacgcg cgtgcgctcg cggaggctgc
     3961 gatcgcgcgc gatccgcagt ccgcaccggc acagctgctg ctgtcgcgcg tgctcgcagg
     4021 taccgacgac gcagccggtg cgatcgccgc ggccaatgcc gcgcgccgca tcgatcccgc
     4081 aggtcaggcg ttcgcgctgg ccgaaatcct ggtcgcgctc gatcgcatcg aggaggcccg
     4141 catggaactc gagcgcctgc tcgcggacga cgacgcctca ccggaagccg agcggcgtct
     4201 tgcgctgctc gcctttcgcg agggcgacac gcaggaggcc gccgggcgat tcagccagcg
     4261 cctgcggacc ggcgacggtg cgggcgaggc gctgttctac ctcggcgcgc tcgccgagcg
     4321 ccacggcaac gacccggtgg cgctcgagat gtatcgccag ctcgtgcagg cgggcgccgg
     4381 catgctggcg cggcgccgcg cggcattcgt gctgatgaag atgggcaagc gcgaggaagc
     4441 gttcagcctg ctcgacgccg aagtcgcgac cgagcccggg accgcgatcg atgtcgtgct
     4501 cgccaagagt tcactgctga tcggtgccga cgatgccgcc gccgccgtgg atctcctcga
     4561 tgccgcgctc gatctctatc cgggacatcc cggcatcgag taccagcgcg cgcttgcgct
     4621 cgagaacgcc gggcgcgtgc cggaatcgat tgccgccttc gagacgatgc tgaagcgtcg
     4681 ccccgaagat ccggtgacga tgaacgcgct cggctacacg cttgccgaca acgacaggag
     4741 cctcgcgcgt gccaacgccc tgatcgacga cgcacttgcg ctcacgccgg acaacccggc
     4801 gaccctcgac agccagggct gggtccgctt ccgccgtggc cagggtgagc aggccctcga
     4861 atcgctggcg cgggcgtacc gcctgtcgcg cgacaccgag atcgcggcgc actggggcga
     4921 ggtgttgtgg tcgctcggac gcgagagcga ggcacgcacg atctgggcgc gcgcgctcgc
     4981 gcgcgatccc ggctccacgc tgctgaagga cacgatcggg cgattcatcc cgcccgctgc
     5041 gccgtgaccc gccggcttcg ccgccacttc gcccgtaccg cgttcgtcgc ggcactgggc
     5101 gcgctactcg ccgcgtgcgt cagcgcgccg gtgcgcgact cgtcggcggt gccctggccc
     5161 gagcggcgtg cagcgctgca ggagcagact gaattcgggc tcaccgggcg tgtcgcggtc
     5221 aactccgcgg gcgcgggctt caatgcttcg ctgcgctggc aacagcaggg cgggctgtcg
     5281 caggtgtcgc tcgatggccc gctcggcgtc ggcggggcgc agatcgaggt ggccgatggg
     5341 catttcgacc tgcgcacctc gcggggcgag catctcgagg gcgacgcggc gcgcgcggcc
     5401 ctggaagaac ggctcgggtt tccgctgcct ctcgccgagt tgcgctactg gatgcgcggc
     5461 gtgccggcgc ccggcgccgc ggccgacgag gtgatcgacg aggcgcggca gcgactcacg
     5521 cgcctcgtgc agcagggctg ggagatcgac tacggcgaat acctcgaagg cgcgggcggc
     5581 agcctgccgc gacgggtcat tgcgcagcgt ggcggcacgc gcgtacgggt cgtcgtcgac
     5641 cgctggacgc catgagtgcg ccgcatgcgc agcgcgagcg ctggccggcg cccgcaaagc
     5701 tgaatctttt cctgcacatc accggccggc gtgcggatgg ctaccatacg ttgcagacgg
     5761 ccttccagct gatcgacctc gccgactcga tcgagatcga ggcgaccagc gactgcgaga
     5821 tccgccgcct cgaggggccg gacggtgtgc ccgccgaggc cgacctggcg gtgcgcgcgg
     5881 cccgcgcgct acaggtcgcg accaccgtgc ggcgcggtgc gcgactgcgg atcgagaagc
     5941 ggatcccggt gggggcgggc ctcggaggcg gcagttccga cgcggcgacc acgctggtgg
     6001 ctctcaacga attgtgggga acagggctcg atctgcggca attagcatcg attggcgccg
     6061 atcttggtgc ggacgtgccg ttgttcatcc atggccgctc ggccttcgcg gagggcatcg
     6121 gcgacctgct gatgccgctc gaactgccgg aaaagtggtt tctggtggtt tatccgggcg
     6181 ttgcagtggc cacggcagcg gtgttccaga gccctgaatt gacacgaaat tcccccctca
     6241 tcacaatacg cgaccttttt ggggcggaga cgcacaacga ctgcgagccg gtggttcgcg
     6301 cgcggtttcc ggcggtgggt gaagcgctcg actggctcgg ccagtttgcg ggagcgcgac
     6361 tcacgggaac cggctcctgc gtcttcgcgt cgttcgacag cgcgagtgcc gcggggcagg
     6421 tcgcggcgaa ggtgcctcag tcgtggcgca gctacgtggc gcgcggcttg aaccactcgc
     6481 cgctgcacgc acagctcgcg cgactgcgag gcgagtcgga gtagggaact ggttttccgc
     6541 tggggcgtcg ccaagtggta aggcagcggg ttttgatccc gccatgcgca ggttcgaatc
     6601 ctgccgcccc agccagagga atagcgaggt tgaggtgggc gattcgacgt cgatggcgct
     6661 gtttgcgggt aacgccaatc gtgcgctcgc gcaggacatc gcgcgtaacc tgaagcagcc
     6721 gctcgggcgc gcctatgtcg gcaagttcag cgacggcgag gtcaatgtcg agctgatcga
     6781 gaacgtgcgc gggcgcgacg tgtatctgct gcagccgacc tgcgcgccgg taaacgataa
     6841 cctgatggag ctgctggtga tggccgatgc ctgccggcgt gcttcggcgg cacgggtgac
     6901 ggcggtggtg ccgtatttcg gctacgcgcg ccaggatcgc cgtccgcgtg cgacgcgctc
     6961 ggcgatcacg gcgaagctgg tcgcgaacat gatttcgggc gcgggcgtcg atcgcctgct
     7021 gaccgtcgat ctgcacgcgg accagatcca ggggtttttc gatatccccg tggacaacgt
     7081 gtacggctcg ccggtgttgc tcggtgatgc atggaggcag ggctacgagg acatgatcgt
     7141 cgtatccccg gacgtcggcg gtgtggtacg cgcacgggca ctcgccaagc ggctcgatga
     7201 cgcggacctc gcgatcatcg acaagcggcg tccgcggccg aacgagtcga aggtgatgaa
     7261 catcatcggc gaggtggcgg acaagacctg cgtgctcgtc gacgacatgg tcgataccgc
     7321 gggcacgctg tgcctcgcgg cacaggcgct caaggaccag ggcgcgaagc gcgtggtggc
     7381 gtacatcacg cacgcggtgc tctcgggcag cgcggtgcag aagatttccg ggtcggcgct
     7441 cgacgaactg gtcgtgaccg atacgattcc gctgaccgag gcggcccgcg attgcggccg
     7501 catcaggcaa ctgtcggtgg cggggctgct ggccgagacc atccgtcgca tacgcgacga
     7561 ggagtcggtg agcagcctgt acatagactg attttggtgt ccggccttct ggtcgcggaa
     7621 gtgccggttt cgttaacgtt aaggagaagt tcatgcgtat cggattcgag atccaggcgg
     7681 acttccgtga cgatcagggc aagggtgcga gccgccgcct gcgtcgacac ggcaagattc
     7741 ctgccatcct ctacggcggc aagctcgcgc cccgccagat cgccctcgac catcagcaca
     7801 tgctgaccct ggtcgacaac gagaagctgt attcgtcgat catccgcctc aaggtcggcg
     7861 acgagaccca ggaagcgatc atcaaggacg tgcagatgca ccccgcgaag aacgcgatcg
     7921 tgcacatcga catgctgcgc gtgctggcgg acgagaagat ccgcatctac gtgccgatcc
     7981 acttcaaggg agcggcggcg gcgccgggcg tcaagacaca gggcggcgtc gtgtcgcacc
     8041 gtatctccga cgtcgaagtg atctgtctgc cgaaggactt cccggaattc ctcgaggtcg
     8101 acatgtccga gatgcagctc aacgagacca agttcctgtc ggacatcccg ctgccggaag
     8161 gtgtcgtgat cccgcagctg cagcacggcc gcaatctggc ggtcgtctcg gtgcatgcgc
     8221 cgcgcgcggc ggagcccgag ccgaccgagg cggcggctgc ggccgaaggt gcggtggcgg
     8281 cggcgccggc tgccgaggcc ccggcgaagg aaggcggcgg caagaaatag tcgccgtgtg
     8341 cgcctcagca aaggccgccg gagtccggcg gcctttcgct ttttctggcg cacgatgcac
     8401 tgaggagcgc agcgatggcc gaatttccgc tcaagctgat cgtgggtctc ggcaatccgg
     8461 gcgatgaata cgcgcgcacg cgccacaacg ccgggttctg gttcgtcgag gaactcgcgg
     8521 cgcgttacgg cggcacgatg cggagtgatc gccatcaccg cggcgagacg gcgcgcgtgc
     8581 ggatcggcga cgccgaagtc ctgctgctga agccgatgca gtacatgaac cgcagcggcg
     8641 ccccggtggg cagcatcgtc gacttctaca agatcgacgt cagctcgatt ctcgtcgcgc
     8701 acgacgaact cgacctgccg gtcggcaccg tgcggctgaa ggagggcggg ggccacggcg
     8761 ggcacaacgg tctgcgcgac ctgattccgc gcatcggcga gggtttctgg cggctgcgca
     8821 tcggcatcgg ccaccccggc gacaaatccg aggtcgtcga ctacgtgctg acccgcgcgc
     8881 cggcgggcga ggacgccacg atccgcgcgg ccgtggcggc cgcggccgaa gtggtgccgc
     8941 tattgctggc cgagggcccg cagaaggcga tgcaccgcct gcatacgaac gtgaaggact
     9001 gaatccagcg atggcaatca agtgcggcat cgtcggcctg ccgaacgtcg gcaagtcgac
     9061 tctcttcaat gcgctcacgc gcgcgcagat cgcggccgag aactacccgt tctgcacgat
     9121 cgacccgaat gtcggcgtcg tgccggtgcc ggatccgcgg ctcgatgaac tcgccgcgat
     9181 ccagaagccg cagaaggtgc tgccgaccac ggtggagttc gtcgacatcg ccggcctcgt
     9241 cgccggcgcg tcacagggcg aggggctggg caaccagttc ctcgcgcaca ttcgcgaggt
     9301 cgatgcgatc gcgcatgtcg tgcgctgctt cgaaaacgac gacatcgtgc acgtcgcggg
     9361 caaggtcgac ccgctggcgg acatcgagac gatcgacacc gagctcgcgc tcgccgacct
     9421 gcagagcgtc gagaaggcgc tcgaccgcgc ggccaaggcg gccaaggcgg gtgacaagga
     9481 ggcactgcgc aagcgcgacc tttacgagcg cgtgcgcggg cagctcgatg ccgcgaagcc
     9541 ggtgcgcgcc atgggcctcg gcgcggatga gctgcgcgac ctgcaagatc tgcacctgct
     9601 gaccgccaag ccggtcatgt acgtggcgaa tgtcgatgaa ggcggcttca ccgacaaccc
     9661 ccgcctcgat gcgctgcgcg cgcgcgcggc gagcgagggc gcccaggtgg tcgcggtgtg
     9721 cgcggccatc gaggccgaga tcgcgcagct cgacgacgac gcgcgcgccg aattcatcgc
     9781 cgacctcggc ctcgatgagc cggggcttaa tcgcgtgatc cgtgccgcgt acacgctgct
     9841 cggtctgcag acctatttca cggtcgggcc gaaggaagtg cgtgcatgga ccgtcaggcg
     9901 cggcgcgacc gcgccgcagg ctgcggccgt gatctacacg gatttcgagc gcggcttcat
     9961 ccgtgccgaa gtggtcggct acgacgactt catcgcctgc aagggcgagg cgggcgcgaa
    10021 ggaggccggc aaactgcggc tggaaggcaa ggagtacatc gtccgcgagg gggacgtgat
    10081 gcactttcgc ttcaacgtct gagcaaaaag ggcggccgcc aagcgctgga actgcgcttc
    10141 acggatggtc gcgaatcgct tgcggcgctc gcgcgcactg cggcaccatc cctcctccca
    10201 ggcatggagc ggaggcgatc atgtgcgacc acgactcatt cgacgacatg gtcgagtacc
    10261 agctcagatc gaaaggcctc tcgcggcgcc agttcagcgc gctgacgctc ggggcgggcg
    10321 cgctatcggt gtttccgccg ctcgccggtg ccgccgaggt ggtcgagtct gaagtcgaga
    10381 tcgaaacgcc cgacggcacc gcggacgcct gtttcgcgca tcccgcgcag ggcgcgcatc
    10441 ccgccgtgct ggtctggccc gatatcttcg gcctgcgccc ggcgtttcgg cagatgggcc
    10501 ggcggctcgc cgagtccggt tatgcagtgc tgaccgtcaa tccgttttat cggacgcaga
    10561 aggcgcccac tgcgccggag aaccctgatt tcaacgaccc cgcgacgcgc acgtcgctga
    10621 tgaccttgcg tgccacgctg tccgcggcca ccggtgcgac cgatgcccgt gccttcgtca
    10681 cttggctcga tgcgcagccc gccgtcgaca agcggcgccg catgggcacc acgggttatt
    10741 gcatgggcgg tccgctgacg atgtacacgg cggcggagcg gtccgatcgc atcggcgcgg
    10801 gcgcgtcgtt ccatggcggc gggcttgcga ccgacaagcc ggacagcccg catctgctcg
    10861 tgccggacat gaaggcgcag ttcctgatcg cgatcgcgga gaacgacgat cagcgcgatc
    10921 cggaggggaa ggacacgctg cgcaaggctt tcgcggccgc cggtctgccc gccgagatcg
    10981 aagtgtatgc cggcacgaag cacggctggt gcccgcccga cgctgcggtc tacgaccacg
    11041 atcaggccga gaaggcgtgg gcgcgcctgc tcgcgctgtt cgagcgcgcg ctggcgtagg
    11101 gcacggcgct cgtgagcgcg gcctgatgct gcgtggacga tgcgtcgtgc gcagtctttg
    11161 gcgcacgcca gaggagaccc ccatgaagat acgagacctg cccccactcc gtcgccgcga
    11221 tgtcctcaaa gctgccggag cggtcgctgt attcggcact ctgccgttcg ccgggcgcat
    11281 cgcggccgcc gacacgccga agccgaagat cggcatgatc ggttccggcc gggtcggcag
    11341 cgccctcggg cgcgtctggg cccatgcggg ccacgaagtc atgttttcct cgcgcaacct
    11401 cgaagccgac agggaactgg ccgccgccgt cggtgcgaac gcgcgtgcgg gcacctcggc
    11461 agaggccgcg gcgttcggcg aagtgctcgt gttcgcggtg ccttacggag cgctgcccga
    11521 actcggccag gcgctcgcgg agcagatccg cggcaaggtc gtgatcgatg cctgcaatcc
    11581 ctttccgaat cgtgacggcg agatcgcgaa tcgcgcgctc gagcgtggcg cgggggtcgt
    11641 ctcggccgag ctgctggcgg gcgcgcgcgt cgtgcgcgcg ttcaatgcga tcaatgcgag
    11701 gcgcatgggc agcgcgcacg aggagccggg gcgcatcggc atgccgatcg ccggcgacga
    11761 cgaggaggcg atccggatcg cctcgatcct cattcgcgag atcggctacg aaccggtact
    11821 ggtcggcggc tcgggcatgg gcaaatacct catgcccggc acgccgctcg ccggcgagca
    11881 cacggctgcg gagatccggc agatcgccgc cacgcttcgc taacgcttca tcctgaccag
    11941 catgcgggtc acggtgaagt cgcccgtgcc ggcatcgaaa tcgccttctg cgccgaggcc
    12001 caccacccgc gatgtgccat cgagctcgtc cgcaagcgcg gcggtgaagt cggcgaatgt
    12061 ttcgaagttg tcgacttcgt ggccatcacc gatcgcgaac gtgccggtgg cctcggcagt
    12121 cggcacgatc cgcagcggcg tggccagctt ctcgacgtcg tggatcgtgg tgcctgtctt
    12181 gatccagtgc ggccagctga tcgcgctgtt cgcagggtcg atgacgatgg cattcgcgtc
    12241 gaggctcgtg aatggcgcag cggtgcctcg cggccagtag ccgatcgcga gcttcgccat
    12301 cacgtcctcg aagtcgacga ccgttcgcgc ggtgaagtcc ggcggcgccg cgccaaaggg
    12361 ggcgacgaag ccgaagaggc gcgccggcga gccgacgtcg aagcgcgcga ggtcgagcgc
    12421 gccggcatcg acttcgtagg ccgcgggatc cgcgtcctgc ccgggcgtgc cggtaccggt
    12481 gaagtcgaaa cgctcgacgt gccagcgatc gatcgaatcg aggtcgagtg tcagcgtggt
    12541 gccggcgacg cccgtgacgg tgccggcgag ctgggtcacg tcgaggcgca cgcggccggc
    12601 ggtggcatcg atcgtgacaa cgtcgtcgtc gtcgatctgc cgttcgctgc catccgcgac
    12661 ctgagcttcg tcactgtcat cgcccctcga ggcgacaccg aaaaccttga cgcgctggcc
    12721 gacggagatc gcgccgatat ccacatcgcc ggtttcgccc gcgcgggtca cgctggtttc
    12781 gggaccgacg agcacggtca cgggatggcg catgatgagc cagtcgggct tgtcgtggcg
    12841 ggacatgcgc ccgccgctca gcatcagggt gtcgcccgcg cgcgcaacga cattgcccac
    12901 cgcgaaatcg aggtcgtcgt cctcgacgct cgagccggcg agcacgcgat cggcggtgaa
    12961 cacgtggtcc cgcttcgaca gcgtgccgaa cgcggcggtc agcgtgccct cgggcagggt
    13021 ggcgagcgcc gcgaggccgt cggcgcccac ataggcctgg ccgtcgagct cccaggcggt
    13081 gtcggcggtc gtgtgcacgg tgacgcggcc gagctttgcg ctcttgtgcg agaacgggcg
    13141 cacgtccact tcataggtgc ccgcgtcgat atcgacactg acgagcggcc cgcgcacgcg
    13201 cagttcgcgg ctgtcgggcc tgtcgaccgt agcgacgatt atcggcgcgg gagtcaccgt
    13261 gaccgggtcg gcctcgagat cgaccgagtt cgacaccgca aggtcgaagt cgagctgcag
    13321 cagcgcgggg cgcccgttcg agacgacgag gtgattgcgg ttgtcgaggc gcacctcgac
    13381 gtcgatcacg ccgaccggct caccgtcctc gtcgatcacc gtggcctgca ccggctggcc
    13441 ggcgacctcg acctggatat cggcgttcgt gtagtcgagc cggatcgtgc cggcgacata
    13501 ggcgccctgc ggcacggtcg cggccgtgac gaactcgctc acgccgacga gctggctgaa
    13561 atcgatgcgc gaggtgacgg gcagtgtctg cacgacctga ccgttggccc gggtgagcgt
    13621 gagcgagacc acatcgacgg agtaggcgag gaagtcgccg tcggcatcgg tgatcgtgat
    13681 catcgccatg ccgcagtcgg gtgcgccgca gctgccggtc gccgggccgc ctgccgtggg
    13741 cgcgggcgtg ctgccgccac cgccaccacc gcagctgttc agagccaata agagaagggg
    13801 agcgaggaac ggtgaaatgc gcttcagggg gggcatggca gtaccctttg cagcttcccg
    13861 actccgggga gggcgaggta tacgccgatc cgggcgcggt tgcagcctgt ctccgggcaa
    13921 cgacggtttc ggggtccgtc ggcttgactc accgggagcg ggtccggaca atcgccggcc
    13981 cgcctgtgga aaggtagctc agccggttag agcacggcac tcataatgcc ggggtcgagg
    14041 gttcgagtcc ctccctttcc accactccaa cctcgtctag ccgttcttcc cgcgcgcgtg
    14101 ccaggccgcg agcgtcgtac gttcgagctc cgggtcgagt ccggcgcgca gcttctcctg
    14161 gcgctccgcg gggcgcgtgc gatgccacgc cagggtatcg cgcacggtgt cttcgagcgg
    14221 gcgcgtcctg agccccgcgg cctgcgcgcg atcgctcgcg gtgagcgacg cgcccgcata
    14281 ttcaccgccc atgggtgacc agggaggcag gtcgacctgc tcgggttgcc actgcttgaa
    14341 gaggaagtcc tccggcaccc aggtggcctt cgtgcccgcc tgcggcgagg ccgcgatact
    14401 cgcgcgcacg atgtcgccga tcgtatattc gcgcgggcgc gtcactgcat tgaaatagcc
    14461 ggtcacgcgc cgctcgacga gcgcgagcat ccaggcggtc aggtcgcgcg cgtcgatgat
    14521 ctggatgggg tcctgcggtg tgcccggcgc aagcatctcc ccgccgcggg ccgcacgcac
    14581 cggccagtac gtgaagcggt cggtgggatc gagcgggccc acgatgtagc ccggtcgcac
    14641 cacgctcgcc cggccctcga acgcctgcat cgagtactgc tcgcacagcg ccttcatcgg
    14701 gccgtagctc tcgttggtga ccgcttcctc ctcggggttg gccagatggc cggtgggcga
    14761 ggactcgtcg ttcggtttcg cgaaactcgc ataggccgag atgctcgaga cgaacaggtg
    14821 gtagccggcg ttcggcgcca gcagttcagc cgacatcttc acgtatttcg gtatgtagcc
    14881 cgtgttgtcg atcaccgcgt cccaggcgtg gccgcgcagc gcgtcgagct ggcctttgcg
    14941 atcgccgtgc agtgtctcga cgcccggcac accgtcggcg tcgcgcttgc cgcgattgaa
    15001 atgcgtgacg gcccagccgc gctgcagcgc ctgttcagtg aggtgcgggc cgaggaaacc
    15061 ggtgcctccg aggatcagca ggcgcagcgg cgccgcttgc gctgcgaccg accggtgcac
    15121 ggcggcgagc gaggcggcac cgagtccgag cttcatcagt tcgcggcggt tccacatggt
    15181 ttcactccat tcagtgcagg gcgtagaggt tgccgtccag gctgccgaca tagatgacat
    15241 cgtccgcgac ggtcggcgag gacaggatgc cgccgatcga gcggaagcgg aagaggtcga
    15301 tgtacatgtc gcgaaaatcg cggaacacgg gcgcgaagcc atcgcggtcg aagctgccgt
    15361 cgggtttcag cagcttgagc ggatcctgtt tcgaggcttc ggtctggaaa gtccaggcga
    15421 gccggccatc gtccgcgtcg agcgcgtaga gccggccatt gtggctgccg aagtagaccc
    15481 gcccgccggc cagcgccgcc gacgagaaca cgaagccctt cgcatcgaaa ttgaatcgca
    15541 ggcgtccgct cttcgcgtcg atcgccatga ggcgcgaggt gtcggatgtg ccgacgtaga
    15601 cgatgccgtt gcgcaccgcc ggcgtgccgt tcacccacga cttgctcgtg gggtagtccc
    15661 agcgcttgcg cccggtcttg gcgtcgagcg cgtagacgtg ggcgtcgcgg caaccgacgt
    15721 agaccacgcc gtcgacgacg gcggccgacg actggaagcc gacctggttg tggttggccg
    15781 gatcctcgcc ggcctggaag atccacttcg actggccggt ggcggcatcg agcgcatgga
    15841 agtcgctggc gaagctgccg acgtacaccg tgccgtcgac gaccgttggc gaggcgtgca
    15901 cgacgccccg catcgggaac ttccactgca gcacgccact gcgcgcatcg acggcgtaga
    15961 cgttgccgtc gccactgccg aaatagacct tgccgtccgc cacagcgggc gaggatgtat
    16021 atacatccca tgcgtcggca atggtctggc cggccgggcc ataaccgtgc aggcccctgg
    16081 cctcgaaatg gcgttcgtgc tcggtggcga agatccagcg caactgcccg gtcgcggcat
    16141 cgagtgccac gagcgagccg gccgacgaga cgaaatagag cgtgtcgccc tccaccgcgg
    16201 gggtcgaggc gatcggcatg cgcgacacga acttccacag ctcgcgaccg ctgtggcgat
    16261 cgatcgcgag cagtgcaccg ctcgcgctgg cgatgtacac cagatcgccg gcgacgaccg
    16321 gtgaggcgag caccgggccg ccggtgtcga aggaccactt gacgccctgc agttgcgccg
    16381 ggccactacc cgcgtaaacg ccggtgcgcg cgttgtcgcc gtgaaacgtg gcctgcgccg
    16441 cggcgcccga agcgcagccg agcagcacgg caagcatcgc gatgcgtttc atgcaccgca
    16501 cgatatcgcg cttcgtcgca gccgccgggg cccggacaat cggttcggcg tgatttcggt
    16561 gcgcctcgcc gcgtctacag ccactccgcc agcaattcga gtgcgcgttc gatttcctcc
    16621 ggtgtcacgg cgaacgagaa gcgcacgaaa tggcgccccg cgtccggatc gaagtcgatg
    16681 cccggggcaa ggccgactcc gatctcgtcg acggcacgca ggcagaactg catgctgtca
    16741 ccggtgaggt gccccacgtc ggcatacagg tagaacgcgc cgtcaggcgg cacgatgcgc
    16801 g
//
LOCUS       DAOQVY010000047        16562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_3969_length_16562_cov_6.532077, whole genome shotgun sequence.
ACCESSION   DAOQVY010000047 DAOQVY010000000
VERSION     DAOQVY010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16562
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3969_length_16562_cov_6.532077"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PKN91_06205"
     CDS             complement(<1..529)
                     /locus_tag="PKN91_06205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNS27698.1"
                     /translation="MGSGRERAILHVDMDAFYASIEERDEPALRGKPVIVGGTSGRGV
                     VAAANYVVRRFGVHSAMPMGRALQLCPQAICIAPRMSHYQAVSREVFAVFHEITPLVE
                     GLSLDEAFLDVTASRALHGDEVAIARTIKARILERTQLKASVGVAPNKLVAKIASDLE
                     KPDSLTVITAAELPGR"
     gene            complement(529..1728)
                     /locus_tag="PKN91_06210"
     CDS             complement(529..1728)
                     /locus_tag="PKN91_06210"
                     /inference="COORDINATES: protein motif:HMM:NF023828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HNS27699.1"
                     /translation="MSAEVEGGEDDAITGDVVAVAVPVAVPVATPEPATALVSDHDGD
                     GGGDLAPLVEAPHCLNCDAPLAGRYCAACGQRDDPHAHRLGHFVREAFEGLTHADSRL
                     WRTLWALLARPGFLTREFFAGRRQRYLPPFRLYIVVSLLFFIALTLLPETPPILSFSD
                     VAPTRPQVAAQAGQIADLAEQIAAEKDPERRSALIIGQQALAAKDAREAAERIPEAVP
                     DPAPATDGGEAAPPPGRAEKICDSLNYAGPFRSWVAPRMRLGCIKAVEDEGSLGRAFL
                     HNIPRAMFVFLPLIAAFMLLIYWRPRRYYVEHLLFYIHNHAMIFLLGTLLIPVFLLLR
                     GTVGVAILGWAVFLWVVWYLYRAMRVYYAQGRGRTLLKYLFMGNVYLVASITTLVLTA
                     LVSVVTL"
     gene            complement(1725..4259)
                     /locus_tag="PKN91_06215"
     CDS             complement(1725..4259)
                     /locus_tag="PKN91_06215"
                     /inference="COORDINATES: protein motif:HMM:NF009586.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNS27700.1"
                     /translation="MITLVLVAAAIFAVTKVGGIITLLFVAAVLALAYKRLSLIAFAA
                     TFTVLLAAYTALGHPAGIWKGFLWVMLALLWLLTIRPLRKSLITRPFMKTYLRMLPSM
                     SQTEREALEAGTVWWDGELFTGAPDWKKLSATHTPTLTAEEQAFIDGPCETLCLMLDD
                     WDITHLRGDMPPEVWDFLKTQGFFAMIIPKKYGGLEFSAYAHSCVLTKLASRSATASS
                     TVAVPNSLGPAELLNHYGTEEQKDYYLPRLARGEEVPCFALTGPRVGSDAAALPDTGI
                     VCRGMWQGREIIGLRLNFSKRYITLAPIATVIGRAFRMFDPDKLIGDVEDIGISCALI
                     PRDTPGLTIGRRHLPLNIPFQNGPIEGRDIFVPLDVLIGGTRMAGQGWRMLIEQLSVG
                     RCISLPANATGGAKAAVFATGAYARIRRQFNMPVGKFEGVESVIARMVGHTYIIDAAR
                     SVTTGAIDGGEKPAVPAAILKYHATELARKIGDDAMDVHGGKGIMLGPNNYLGRGYQA
                     IPIAITVEGANILTRNLIIFGQGAIRCHPFVMREMNAAHNPDRKAGVDEFDRALFGHM
                     GFAISNAVRSLVMALTFARFARVPAQGPTARYYQHIVRFSASFAFAVDVAMLTLGGYL
                     KKKESLSARLGDVLSSMYLASMVLKHHENQGKPEADLPIVEWACRSLMYTAQEQLHSF
                     LRNFPSRFLAGCMRLLIFPRGLTYFAPGDRLGRKLAELVTQPSESRDRLCQLVYRTLE
                     PGNALGLLQEALVLAQTAEPIEKRLRVEGVKTGRVTALDLPGQIRQGLELGILSETEA
                     ALLRDYDRKVMHLVNVDDFESHELGTRAQPGSADTRDAGLAGAARA"
     gene            complement(4281..4817)
                     /locus_tag="PKN91_06220"
     CDS             complement(4281..4817)
                     /locus_tag="PKN91_06220"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HNS27701.1"
                     /translation="MIRTLAAVCLLAFAAIPAANADSKEELDAKVREAMTEFAKHTSA
                     GAELAAKAKGVLVFPSVIKAGFGIGGEYGEGALQIRGATVDYYNIASASIGLQLGAQA
                     RTQIVLFMTDEALRTFRDSKGWKAGVDGSVALATLGAGGTIDTKTAQEPIIGFIFSNK
                     GLMYNLTFEGSKMTRIDK"
     gene            complement(4814..5794)
                     /locus_tag="PKN91_06225"
     CDS             complement(4814..5794)
                     /locus_tag="PKN91_06225"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNS27702.1"
                     /translation="MKTALNVAITGAAGQIGYALAFRAASGALFGPDQPVNLHLLEIT
                     PALPQLTGVVMELNDCAFPTLQKVVATDDAKVAFRDCHAALLVGARPRGPGMERKDLL
                     LANAQIFSAQGKALNEAADRNVKVLVVGNPANTNALIARANAKDLNPRNFTAMTRLDH
                     NRALSQLAEKTGTHVSQIQRMIIWGNHSATQYPDISHALIGGRPAKSLVDQDWLENTF
                     IPVVQQRGAAVIKARGASSAASAASAAIDHIRDWFRGSPAGDYLSMAVPSDGSYGIPE
                     GVIYSYPVTTSNGEYQIVQGLSIDEFSRQRMDATLAELREERDGVKELLT"
     gene            5995..6153
                     /locus_tag="PKN91_06230"
     CDS             5995..6153
                     /locus_tag="PKN91_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27703.1"
                     /translation="MSTTTHNFAVAVGAAVLAIVLTVASSITFVNATSVARVTVDAPE
                     AIHLAARN"
     gene            6220..6624
                     /locus_tag="PKN91_06235"
     CDS             6220..6624
                     /locus_tag="PKN91_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNS27704.1"
                     /translation="MDPQWDDSLPIYRQLRDRVVAMILEGVLKEGDPLPSVRNVAAEF
                     RLNPLTVLKGYQQLVDEQLVEKRRGRGMYVNAGARKALMKDERQRFIEDEWPKVYATI
                     QRLGLSTDELFAQTPAVTQTLSTAGSTDDKHE"
     gene            6608..7480
                     /locus_tag="PKN91_06240"
     CDS             6608..7480
                     /locus_tag="PKN91_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS27705.1"
                     /translation="MTSTSDTPVIEARKLAKSYGKLRALDGIDFKVGAGRIVGLIGPN
                     GAGKTTALKAILGLTPFEGELRVLGRDPSAARDALMQDVCFIADVAVLPRWLRVRQAL
                     DYVAGVHPRFDRARAEAFLATTDIKMKSRVRELSKGMVTQLHLALVLAIDARLLVLDE
                     PTLGLDLLYRRQFYDTLVNDYFDKDRTILMTTHQVEEIENLLTDVIFIQHGRVVLDAA
                     VEDLGGTFRQLICAPDQMERARALTPFYSREIFGRTAFFYEGRDAAELAPLGELRTPS
                     IADLFVAKMQGVAP"
     gene            7477..8364
                     /locus_tag="PKN91_06245"
     CDS             7477..8364
                     /locus_tag="PKN91_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27706.1"
                     /translation="MKLYPILVRREFWEHRALWVAPLAMAALWIATSAFMKIHMGNEL
                     PLTVAQGRAILNVVGWGMSVATLLVVGVVVVFYLLDSLYAERRDRSILFWKSLPVSDA
                     ATVLAKLAVAMVIVPLLAFVVVAFTDLIARALIAARGGAGITVEQASIWDGRMWWQAQ
                     VLLFIGIVASVLWYAPIAAYLLVMSAWARRSVMLWAVLPPLVLMMVEGMVLRTTRVWE
                     FISDRLSPAVPIMRGIEAQLASGTIKIDRNEVVLPGALLDHVDLGAIFLNAQLLLGLL
                     AAAVLVFVAIRLRRYRDET"
     gene            complement(8346..9101)
                     /locus_tag="PKN91_06250"
     CDS             complement(8346..9101)
                     /locus_tag="PKN91_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNS27707.1"
                     /translation="MSHPDRNRRRHGASLLIATLLALGAHMPVAGADGPLALDLRRIV
                     PITGDAKAGEAKAELCATCHGKHGIGIVPMFPNLAGQRPDYLYWELMSYKHGARPQSG
                     MTPLVELLSEEDMRNFSMYYATMVPPPRAADADATPADPELLRLGEQIYMHGDAALGI
                     PPCQGCHGADARGHPRALQVDSAGYTPFAVFPALRGQHAEFLQATLEEFRIGERSGST
                     VDRVMNGVGKQLDDRSISALTAWLASLDQVSSR"
     gene            complement(9098..11581)
                     /locus_tag="PKN91_06255"
     CDS             complement(9098..11581)
                     /locus_tag="PKN91_06255"
                     /inference="COORDINATES: protein motif:HMM:NF023282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HNS27708.1"
                     /translation="MLAVCAAGLATAQDSGVGVDLHFGNSLQPNGVAPLACDPDGMSW
                     LTAARKRTPSGHLYLCPPEFSQPRTTGSGNWLFDGTFGLGYLDINDERNTNWLRYDNW
                     DDGVLARLELSLMRPGDGSYFDLRASHINGDNQYLKLNAGRVGHYRIEAYARSQVNVT
                     SGTAKSIWDGVGSDYLALKPGLTPGGSSPAEVSAISAASPERILKVVRDKYGIGINYY
                     IDRQFTAYASASQEERKGARPFGGPFFFAFIFPGSGGVYETPRPIDDSTLSFNGGLRY
                     VGSAWNWDLGYSGSLFRTGESSFSYEVPFAFPPLVPGFGNFPLTLGEFAYEPENDYHN
                     LRGTLTRKLPQNGQLSLTASLSTSRQDDDLRPPMNCNGLFGLTTPPFTFDCANWNTTA
                     ALSRTSADLSIDSTLLNAKLVLQPTSLVTVQGNVKYHRQDYRGDYFAFNPLTGQYGYI
                     AENGAQGSSVPGEMGVWDPALFPSVLTRVRNLPLDKEISEGSVAADWRVSRKDTLSAA
                     LTFTRTERSNREVENQDDLLVKLGWSSRATDDLSLRLNYSYLDRSGDHYNHDPYEFTF
                     STSLPGFVDPGTLPAHTVDAMRKYDVGDREQHKATLIATYVLTGSSTLSANLRGEWNS
                     YDAVIGRLGYDTWSAGLQWDWQPSERTSANAFVGYDHSSLDTANVNDAAANIPDPTLG
                     GPVYPEANRWWLEDEQRNYYAGANLSTHFARGRLELGWDFSDSRGITNYRFNSPGALA
                     RPVLAAAAGNGYAPMLYRINTWTLSWTKAISKRVSVRVFDTYETGRLSDWHYAGLDSS
                     LVTSNRVYLDSGPRDYSVNLIGLMVEAKL"
     gene            complement(11645..12706)
                     /locus_tag="PKN91_06260"
     CDS             complement(11645..12706)
                     /locus_tag="PKN91_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HNS27709.1"
                     /translation="MRAFLGLLSLAAAALLFAGWLAGPVAAQQANPTVSNPYNLPETD
                     LGVGKNDIARWLSAPPGFAHASVPVNPLHPEATVVGQQMCVACHQQENRHFLHTLHAL
                     GMNAALAADPKTPTCEACHGAGSVHSQAPGKPGTIIAFTRDGGTPVAAQSAGCLGCHS
                     GGQRDHWLGSVHQRNELSCSDCHNPMAKFSAEGLLARQSISETCATCHKDIRQQFNRR
                     SHMPLPEGQMSCADCHNPHGTINASLMKTDSVNETCYQCHAEKRGPMLFEHAPVRDSC
                     LNCHTPHGSNQHALLVAPIPFLCQQCHTHVRHPNDLLTTTSLANGPAADERLMARGCI
                     TCHAQVHGSNHPSGPRLHR"
     gene            complement(12797..13990)
                     /gene="purT"
                     /locus_tag="PKN91_06265"
     CDS             complement(12797..13990)
                     /gene="purT"
                     /locus_tag="PKN91_06265"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HNS27710.1"
                     /translation="MTTLGTPGTEAATRLLLLGAGELGKEVTIEAQRLGVEVIAVDRY
                     PGAPAMQVAHRHHVIDMLDPRAPRAVIERERPTFVVPEIEAIATAELVALEAEGCTVI
                     PCARAAHLTMNREGIRQLAAGELGLATSPYRFADSPADYRAAVAAIGLPCVVKPVMSS
                     SGKGQSLVRTAEEIEAAWRHAQTGGRAGAGRVIVEGFVDFEYEITLLTVRHAGGTAFC
                     APIGHRQVDGDYVESWQPHPMSRLALERAQHTARTVTDALGGRGIFGVEMFVRGDDVW
                     FSEVSPRPHDTGMVTMIAQDLSQFALHVRAILGLPIPNIAQHGPSASAAILLEGHGGD
                     IRYAGVEAALAEPDTALRLFGKPEVRGHRRMGVALARGESIEAARAKARAAAAAIVAG
                     AQIAD"
     gene            14040..15164
                     /locus_tag="PKN91_06270"
     CDS             14040..15164
                     /locus_tag="PKN91_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HNS27711.1"
                     /translation="MHLSAEHADGRLTLHLLGEWRALDLTEIRAELAALPLEKVSEIT
                     IDTGAVERLDLSGAWLLREFLDRAEAGGARKVGFSGERPAQLALIDRTRAEDAEAARA
                     RPPMEEAEESPVAAVGRTTVIQWRGIRDGLDFLGRITVQLLRAFGSVKRMRLTSITRH
                     VYDTGITAIPIVSLIAFLISVIIAYMSAQQLRNFGAEIYVVDLVTVGVLRELGVLLTA
                     IIVAGRSGSAFAAEIGAMKLNEEVDALTATGIEPIEVLVLPRVIGLMIALPLLTVIAD
                     GVGIAGGALLCKILLDMPLVQYFARVEDAIASTTFWAGLVKAPVFAILIATAGTYRGL
                     QVRGSSRELGRLTTFAVVQSIFLVILADALFAVLFMEIDF"
     gene            15164..15928
                     /locus_tag="PKN91_06275"
     CDS             15164..15928
                     /locus_tag="PKN91_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS27712.1"
                     /translation="MAAIVEVRDIVNRFGTQVVHDGLDMQVLEGEVFGIVGGSGSGKS
                     VLLRTILGLRKPQRGVVLLEGQDITHQSWNELRAAKAGYGVTFQQGALYSSLTVLQNI
                     QLPMIEHLRLPPDALDALAKLKLRIVGLAEEAGSKYPAQLSGGMVKRAALARALALDP
                     KLLFLDEPTAGLDPISAAAFDELLMALQRQLALTVVMITHDLDTIFRTCNRVGVIVDR
                     HMISGTLEEIVDNPHQWIREYFHGERALRFGERHGT"
     gene            15918..>16562
                     /locus_tag="PKN91_06280"
     CDS             15918..>16562
                     /locus_tag="PKN91_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNS27713.1"
                     /translation="MEREANYTAVGAFVLLISAMAVLFVYWYSDGVERRDYTRYEIYF
                     DGSVSGLSRGSPVRYLGVDVGRVAVMRIDRRSSSRVQVIADIDSEAPVSARTVAQLSL
                     QGVTGLLYIDLIENRGNLKVIDPVPSERYPVIASSRSSFDLFLASLPELVAKANDTLI
                     RVNSILSDQNIDSLQTTLKSIERAAGHFPGMMRDAESLVAELRSSSRDFSATMVV"
BASE COUNT         2605 a         5389 c         5766 g         2802 t
ORIGIN      
        1 gccgccccgg aagctccgcg gcggtgatca cggtcagcga gtcgggcttc tcgaggtcgg
       61 aagcgatctt cgcgaccagc ttgttcggcg ccacgcccac cgaggccttg agctgcgtgc
      121 gctcgaggat ccgcgccttg atggtgcgcg cgatcgccac ctcatcgccg tgcagggcgc
      181 ggctcgcggt cacgtcgagg aaggcttcgt cgagtgagag gccctcgacc agcggcgtga
      241 tttcgtggaa caccgcgaac acttcgcggg atacggcctg atagtgcgac atgcgcggcg
      301 cgatgcagat ggcctgcggg cacagctgca gcgcgcggcc catcggcatc gccgagtgca
      361 cgccgaagcg gcgcaccacg tagttcgccg ccgcgacgac gccgcggccg ctggtgccgc
      421 cgacgatcac cggcttgccg cgcagtgcgg gctcgtcgcg ttcctcgatc gacgcgtaga
      481 acgcgtccat gtcgacgtgc aggatggcgc gctcgcgccc ggagcccatc agagggtcac
      541 gacgctgacg agcgcggtca gcaccagcgt ggtgatgctg gcgaccaggt acacgttgcc
      601 catgaagagg tacttcagaa gcgtgcggcc gcggccctgg gcgtagtaca cgcgcatcgc
      661 gcgatagagg taccagacga cccacaggaa gacagcccag ccgagtatcg ctacgccgac
      721 cgtgccgcga agcagcagga acaccgggat gagtagcgtg ccgagcagga agatcatcgc
      781 gtggttgtgg atatagaaaa gcaggtgctc gacgtagtag cggcgcggcc gccagtagat
      841 gagcagcatg aaggccgcga tcagcggcag gaagacgaac atcgcccgcg ggatgttgtg
      901 caggaatgcg cgcccgagcg agccttcgtc ctccaccgcc tttatacatc cgagcctcat
      961 gcgcggtgcg acccagctgc ggaacgggcc ggcgtaattg agactgtcgc agatcttctc
     1021 ggcgcggccg ggtgggggcg cggcctcgcc gccgtccgtc gcgggtgccg gatccgggac
     1081 cgcctcgggt atgcgctccg ccgcctcccg cgcgtccttc gccgcgagcg cctgctgacc
     1141 gatgatcagc gcgctcctgc gctccgggtc cttctcggcg gcgatctgct cagcgaggtc
     1201 cgcgatctgc ccggcctgcg cggcgacctg cgggcgcgtc ggcgcgacat cgctgaagct
     1261 gaggatcggc ggcgtctcgg gcagcagtgt cagagcgatg aagaagagca ggctgacgac
     1321 gatgtagagg cgaaacggcg gcaggtagcg ctggcggcgg ccagcgaaaa actcgcgggt
     1381 caggaaaccg gggcgcgcga gcagcgccca gagcgttcgc cagagtcgcg agtcggcgtg
     1441 cgtgagcccc tcgaaggcct cgcgcacgaa atggccgagg cggtgggcat ggggatcgtc
     1501 gcgctgcccg caggcggcgc agtacctgcc ggcgagcggc gcgtcgcagt tgaggcagtg
     1561 cggcgcctcg acgagcggcg ccagatcgcc accaccgtcg ccatcgtggt cgctgaccag
     1621 cgccgtggcg ggctccgggg tcgcgacagg aaccgcgaca ggaaccgcga ctgcgaccac
     1681 gtcccccgtg atggcatcgt cctcgccccc ttcgacctcg gcactcacgc gcgtgcggcg
     1741 cccgccaggc cggcatcgcg ggtgtcggcc gagccaggct gtgcccgggt gccgagttcg
     1801 tgcgactcga agtcgtccac gttgacgagg tgcatgacct tgcgatcgta gtcgcgcagc
     1861 aacgccgcct ccgtttccga gaggatgccg agttcgagac cttgccggat ttgtcccggc
     1921 aggtcgagcg cggtgacccg gcccgtcttc acgccctcga cgcgcaagcg cttctcgatc
     1981 ggctcggcgg tctgcgcgag caccagcgct tcctgcagca ggcccagcgc attgccgggt
     2041 tcgagcgtgc ggtagacgag ctggcacagc cggtcacgcg attcgctggg ctgggtgacg
     2101 agctccgcga gcttgcgccc cagccggtcg ccgggcgcga agtaggtgag gccgcgcggg
     2161 aagatgagca ggcgcatgca gccggcgagg aagcggctcg ggaaattgcg caggaagctg
     2221 tgcagctgct cctgcgcggt gtacatcagg ctgcggcagg cccactcgac gatcggcagg
     2281 tccgcctccg gcttgccctg gttttcgtgg tgcttcagca ccatcgaggc gagatacatg
     2341 ctcgagagca catcgccgag ccgcgccgag aggctctcct tcttcttcag atagccgccg
     2401 agggtcagca tcgcgacatc gaccgcgaaa gcgaacgagg cgctgaagcg cacgatgtgc
     2461 tggtagtagc gcgcggtggg gccctgcgcc ggcacgcgcg cgaagcgcgc gaaggtgagc
     2521 gccatgacga gcgagcgcac cgcgttcgag atcgcgaatc ccatatggcc gaacagcgcg
     2581 cggtcgaact cgtcgacgcc ggccttgcgg tcggggttgt gcgccgcgtt catctcgcgc
     2641 atcacgaacg ggtggcagcg gatcgcaccc tggccgaaga tgatcaggtt gcgggtcagg
     2701 atgttcgcgc cctcgaccgt gatcgcgatc gggatcgcct ggtagccgcg cccgaggtag
     2761 ttgttgggcc cgagcatgat gcccttgccg ccgtgcacgt ccatcgcgtc gtcgccgatt
     2821 ttccgggcga gttcggttgc gtggtatttc agtatcgccg cgggcaccgc cggcttctcg
     2881 ccgccgtcga tcgcaccggt ggtcaccgag cgcgccgcgt cgatgatgta ggtgtggccg
     2941 accatgcgcg cgatcaccga ctcgacacct tcgaacttgc cgaccggcat gttgaactgg
     3001 cggcggatgc gggcgtaggc gccggtcgcg aacaccgcgg ccttggcgcc gccggtcgca
     3061 ttcgccggca gcgagatgca gcggcccacc gacagctgct cgatcagcat gcgccagccc
     3121 tggcccgcca ttcgggtgcc gccgatcagc acgtcgagcg ggacgaagat gtcgcggccc
     3181 tcgatcgggc cgttctggaa gggaatgttc agcggcaggt gacgccgccc gatcgtcagg
     3241 cccggcgtat cgcgcggaat gagcgcgcag ctgatgccga tgtcctcgac gtcgccgatc
     3301 agcttgtcgg gatcgaacat gcgaaacgcg cggccgatca cggtcgcgat tggcgcgagc
     3361 gtaatgtagc gcttcgagaa attgagcctg agcccgatga tctccctgcc ctgccacatg
     3421 ccgcggcaca cgatgccggt gtcgggcagc gcggcggcgt cggagccgac gcgcggaccg
     3481 gtgagcgcga agcacggcac ttcctcgccg cgcgcgagcc gcggcaggta gtaatccttc
     3541 tgttcctcgg tgccgtagtg attgagcagc tcggccggtc cgagcgagtt cggcaccgcg
     3601 acggtcgagg acgccgtggc gctgcggctc gccagcttgg tgagcacgca ggagtgcgca
     3661 taggccgaga actccaggcc accgtacttc ttcggaatga tcatcgcgaa gaagccctgg
     3721 gtcttcagga agtcccacac ctcgggcggc atgtcaccgc gcagatgggt gatgtcccag
     3781 tcgtcgagca tcaggcagag cgtctcgcac ggcccgtcga tgaacgcctg ctcctcggcg
     3841 gtgagcgtcg gggtgtgggt cgcggacagc ttcttccagt ccggcgcgcc cgtgaacagc
     3901 tcgccatccc accacacggt gcccgcctcg agcgcctcgc gttcggtctg cgacatgctc
     3961 ggcagcatgc gcagataggt cttcatgaag gggcgcgtga tcagcgactt gcgcagcggg
     4021 cgtatcgtga ggagccacag cagcgcgagc atcacccaca ggaagccctt ccagatgccc
     4081 gccggatgac cgagcgcggt gtacgccgcc agcagtaccg tgaaggtcgc cgcgaacgcg
     4141 atcagcgaca ggcgcttgta ggcgagggcc agcaccgccg ccacgaacag cagcgtgatg
     4201 atgcccccga ccttggtcac cgcgaagatc gccgcggcga ccaggaccag cgtgatcacg
     4261 ggtccgagca tcgcggcgcc ctacttgtcg atccgcgtca ttttcgagcc ttcgaacgtc
     4321 aggttgtaca tcagcccctt gttcgaaaag atgaagccga tgatcggctc ctgcgcggtc
     4381 ttggtgtcga tggtgccgcc tgcaccgagc gtggcgagcg ccacgctgcc gtcgacaccg
     4441 gccttccagc ccttgctgtc ccggaaagtc ctgagcgcct cgtcggtcat gaacagcacg
     4501 atctgggtgc gcgcctgcgc gccgagctgc aggccgatcg aggcggaggc gatgttgtag
     4561 tagtccaccg tcgcgccgcg gatctgcagc gcgccctcgc cgtattcgcc gccgatgccg
     4621 aagccggcct tgatgacgct cgggaacacc agcacgccct tcgccttggc ggcaagttcc
     4681 gcacccgccg aggtgtgctt ggcgaattcg gtcatcgcct cgcgcacctt ggcgtcgagt
     4741 tcttccttcg agtcggcatt cgccgccggt atggcggcga atgcgagcag gcagactgcg
     4801 gccagtgtcc tgatcatgtc agtagctcct tgacgccgtc gcgttcctcg cgcagttcgg
     4861 caagtgtagc gtccatgcgc tgccggctga actcgtcgat cgaaagtccc tgcacgatct
     4921 ggtactcgcc gttgctggtg gtgaccgggt aggaatagat cacgccctcg gggatgccgt
     4981 agctgccgtc ggagggcacg gccatgctga ggtagtcgcc ggccggggaa cccctgaacc
     5041 agtcgcggat gtgatcgatc gcggccgaag cggccgaggc ggccgacgag gcgccgcgcg
     5101 ccttgatcac cgccgcgcca cgctgctgca cgaccgggat gaaagtgttc tcgagccagt
     5161 cctggtcgac gagcgatttc gccggtctgc cgccgatcag tgcgtgcgag atgtccgggt
     5221 actgcgtcgc cgagtgattg ccccagatga tcatccgctg gatctgcgag acatgcgtgc
     5281 cggtcttttc cgcgagctgc gagagcgcgc ggttgtgatc gaggcgcgtc atcgcggtga
     5341 aattgcgcgg attgaggtcc ttcgcgttcg cgcgcgcgat cagcgcattg gtgttcgccg
     5401 gattgccgac gaccagcacc ttcacgttgc gatcggcggc ttcgttcagc gccttgccct
     5461 gggcggagaa gatctgcgca ttcgcgagca gcaggtcctt gcgctccatg ccggggccgc
     5521 gcgggcgcgc gccgacgagc agtgcggcgt ggcagtcgcg gaacgcgacc ttcgcatcgt
     5581 ccgtcgcgac gaccttctgc agcgtcggga aggcgcagtc attgagctcc atcacgacac
     5641 cggtcaactg cggcagggca ggcgtgatct cgagcagatg caggttcacc ggctggtcgg
     5701 ggccgaacaa ggcgcccgag gcggcacgga aagcgagggc atagccgatc tggccggcgg
     5761 cgccggtaat cgcaacgttc aaggcagttt tcatcgagtg ctccggggaa aggggcatgg
     5821 attctacaga aatcaactag ttacggtaat agtgggtggc cggtccctgg gcccccaaaa
     5881 aaatccttca gggggtgttg acactgaact agtgttgtag ttatatataa caccaagacg
     5941 agggccaaat gcatggtgca gatggccaga gacggtaaac caaaggagac gaacatgagc
     6001 accaccaccc acaacttcgc ggtcgctgtc ggcgctgcgg tccttgccat cgtgctgacg
     6061 gttgcttcga gcatcacctt cgtgaatgcc accagcgtgg cgcgcgtgac ggtcgatgcc
     6121 cccgaggcga ttcacctcgc ggcccggaac tgaggcgttc tgctagatta gccctctaga
     6181 gcacggcaac tcgctatgca acaggagaac ccgggaatca tggatccgca gtgggacgac
     6241 agcctgccga tctaccgcca gctgcgcgac cgcgtcgtgg cgatgatcct ggaaggcgtc
     6301 ctgaaggagg gcgatccgct gccctcggtg cggaacgtgg cggccgagtt ccggctgaac
     6361 ccgctgaccg tgctcaaggg ctaccagcag ctcgtcgacg agcagctggt tgagaagcgc
     6421 cggggccgtg gcatgtacgt caacgccggc gcccgcaagg cgctgatgaa ggacgagcgc
     6481 cagcgcttca tcgaagacga atggccgaaa gtttacgcga cgatccagcg cctcgggctg
     6541 tcgacggacg aactgttcgc acagacccct gccgtcaccc agacgctgtc gaccgccggg
     6601 agcaccgatg acaagcacga gtgacacgcc ggtaatcgag gcgcgcaaac tcgccaagtc
     6661 ctacggcaag ctgcgcgccc tcgacggcat cgatttcaag gtcggcgccg gccgcatcgt
     6721 gggcctgatc ggcccgaacg gcgccggcaa gaccacggcg ctcaaggcca tcctcggcct
     6781 gaccccgttc gagggcgaac tgagggtcct cgggcgcgac ccgtccgccg cgcgcgatgc
     6841 gctcatgcag gacgtctgtt tcattgccga cgtggcagtg ctgccgaggt ggctgcgcgt
     6901 ccggcaggcc ctcgattacg tggccggcgt gcatccgcga ttcgatcgcg cgcgcgccga
     6961 ggccttcctc gccaccaccg acatcaagat gaagagccgc gtgcgcgagc tctccaaggg
     7021 catggtcacg cagctgcacc tcgcgctcgt actcgcgatc gatgcgcgcc tgctcgtgct
     7081 cgacgagccg accctcggtc tcgacctgct ctatcgccgc caattctacg acacgctggt
     7141 gaacgactat ttcgacaagg accgcacgat cctcatgacg acccaccagg tcgaggagat
     7201 cgagaacctg ctgaccgacg tgatcttcat ccagcacggc cgtgtcgtgc tcgatgccgc
     7261 ggtcgaggac ctcggcggca ccttccggca gctgatctgc gcgcccgacc agatggagcg
     7321 cgcgcgcgcg ctgacgccgt tctactcgcg tgagatcttc ggccgtaccg cctttttcta
     7381 cgagggccgc gacgcggcgg agctcgcgcc gctcggcgag ctgcgcacgc cgtcgatcgc
     7441 ggacctcttc gtcgccaaga tgcagggagt cgcaccatga agctctaccc gatcctggtg
     7501 cgccgggaat tctgggagca ccgcgccctg tgggtcgcgc cgctcgcgat ggccgcgtta
     7561 tggatcgcga cctcggcgtt catgaagatc cacatgggca acgagctgcc gctgaccgtc
     7621 gcgcagggcc gcgcgatcct gaacgtggtc ggctggggca tgagtgtcgc caccctgctc
     7681 gtcgtgggcg tggtcgtggt tttctacctg ctcgattcgc tgtatgcgga acgccgcgac
     7741 cgctcgatcc tcttctggaa gtcgttgccg gtctccgacg cggccaccgt gctcgccaag
     7801 ctggccgtcg caatggtgat cgtgccgctg ctcgcgttcg tggtcgtggc attcaccgac
     7861 ctcatcgcgc gcgcactcat cgcggcgcgc ggcggcgcgg gcatcaccgt ggagcaggcg
     7921 tcgatctggg atggccgcat gtggtggcag gcgcaggtgc tgctattcat cggcatcgtc
     7981 gccagcgtgc tgtggtatgc gccgatagcg gcctacctgc tggtcatgtc ggcctgggcg
     8041 cgacgcagcg tgatgctgtg ggccgtgctg ccgccgctcg tgctgatgat ggtcgagggt
     8101 atggtgctgc gcacgacccg cgtctgggag ttcatctccg accgcctgtc gccggcggtg
     8161 ccgatcatgc gcggcatcga ggcacagctc gccagcggca caatcaagat cgatcgcaac
     8221 gaggtcgtcc tgccaggcgc gctgctggac cacgtcgatc tcggcgccat cttcctgaat
     8281 gcgcagctgc tgctcgggct gctggccgcg gcggtgctgg tgttcgtcgc gatccggctg
     8341 cgacgctacc gcgacgagac ctgatcgagg cttgccagcc acgcggtcag ggcgctgatg
     8401 ctgcgatcgt cgagctgctt gccgacgccg ttcatcacgc gatccacagt ggagccgctg
     8461 cgttcgccaa tgcggaactc ttcgagtgtc gcttgcagga actcggcatg ctggccgcgc
     8521 aatgccggaa acaccgcaaa cggggtgtag ccggcggagt cgacctgcaa cgcgcgcggg
     8581 tggccgcgcg catcggcgcc gtggcagccc tgacagggcg ggatgccgag cgcggcatcg
     8641 ccgtgcatgt aaatctgctc gccaaggcgg agcagctccg ggtcggcggg ggtcgcatcg
     8701 gcatcggccg cgcgcggcgg cgggaccatg gtggcgtaat acatcgagaa gttgcgcatg
     8761 tcttcttcgg acagcagttc gaccaatggc gtcatgcccg attgcgggcg tgcgccgtgc
     8821 ttgtaggaca tgagctccca gtagaggtaa tcgggccgct gaccggcgag attcgggaac
     8881 atcggcacga tcccgatgcc gtgcttgccg tggcaggtgg cacacagctc ggccttcgcc
     8941 tcgcccgcct tcgcgtcgcc ggtgatcggc acgatgcggc gcaggtcgag ggccagcggg
     9001 ccgtcggcgc ccgcgaccgg catgtgcgct ccgagcgcga gcagggtggc gatgagcagg
     9061 ctcgcgccgt gccgtcggcg gttgcgatca gggtggctca cagtttcgcc tccaccatca
     9121 ggccgatcag gttgaccgag taatcccgcg gaccgctgtc gaggtaaacg cggttgctgg
     9181 tcacgaggct cgaatcgagc ccggcgtaat gccagtcgga cagccggccg gtttcgtagg
     9241 tgtcgaatac gcgcacgctg acacgcttgc tgatcgcttt cgtccacgac agcgtccagg
     9301 tgttgatccg gtacagcatc ggcgcgtagc cgttgcccgc ggcggccgcg agcacgggac
     9361 gggcgagcgc accgggcgag ttgaagcggt agttggtaat gccgcgcgag tcgctgaagt
     9421 cccagccgag ctcgagccgt ccgcgcgcaa agtgagtgct gaggttggcg ccggcgtagt
     9481 agttgcgctg ctcgtcctcg agccaccagc gattcgcttc cggatagacc gggccgccca
     9541 gtgtcgggtc ggggatgttc gccgctgcgt cgttgacatt ggcggtgtcg agactggagt
     9601 ggtcgtaacc gacgaacgca ttcgcactgg tgcgctccga gggctgccag tcccattgca
     9661 gcccggcgct ccaggtgtcg tagccgaggc gaccgatcac ggcgtcgtag ctgttccact
     9721 ccccgcgcag gttggccgac agtgtcgagc tgccggtcag tacgtaggtc gcgatcagcg
     9781 tcgccttgtg ctgttcgcga tcgcccacat cgtatttgcg catcgcgtcg acggtatgcg
     9841 cgggaagggt gcccggatcg acgaagccgg gcagggaggt ggagaacgtg aattcgtaag
     9901 ggtcgtggtt gtagtggtcg ccgctgcgat cgagatacga gtagttgagg cgcaggctca
     9961 gatcgtccgt ggcgcggctg ctccagccga gcttcaccag caggtcgtcc tggttctcga
    10021 cttcgcgatt cgagcgctcg gtacgggtga aggtcagcgc ggcgctcagc gtgtccttgc
    10081 ggctcacccg ccagtcggcg gcgacactgc cctcgctgat ctccttgtcc agcggcaggt
    10141 tgcgcacacg cgtcaggacg ctcgggaaca gcgccggatc ccagacgccc atctctcccg
    10201 gcaccgaact gccctgcgcg ccgttctcgg cgatgtagcc gtactggccg gtcagcgggt
    10261 tgaaggcgaa atagtcgccg cgatagtcct ggcgatggta tttcacgttg ccctggacgg
    10321 tcacgagcga tgtcggctgc agtacgagct tcgcgtttag cagcgtgctg tcgatgctca
    10381 ggtccgcgct ggtgcgtgag agggccgcgg tggtgttcca gttggcgcag tcgaaggtaa
    10441 agggcggcgt ggtgaggccg aacaagccgt tgcagttcat cggcggccgc aggtcgtcgt
    10501 cctgccgcga cgtggagagc gacgccgtca gcgacagttg accgttctgc ggcagtttgc
    10561 gggtcagcgt gccgcgcagg ttgtggtagt cgttctccgg ctcgtaggcg aactcaccga
    10621 gcgtcagggg aaagttgccg aaacccggta ccagcggggg gaatgcaaag ggcacttcgt
    10681 aactgaagct ggactcgcca gtgcggaaga gcgagccgct gtagccgaga tcccagttcc
    10741 aggcgctgcc cacgtagcgc agcccgccgt tgaagctgag ggtgctgtcg tcgatcggcc
    10801 gcggcgtttc gtatacgccg cccgagccgg gaaagatgaa ggcgaagaag aacggtccgc
    10861 cgaacggccg cgcgcctttg cgctcttcct gggacgcact cgcgtaggca gtgaactgcc
    10921 gatcgatgta gtaattgatg ccgatgccgt acttgtcgcg caccactttc aggatgcgct
    10981 ccggcgatgc cgccgagatc gcgctcactt cggcggggct gctgccgccg ggcgtcagtc
    11041 ccggcttcag cgccaggtaa tcgctgccga cgccgtccca gatcgatttc gcggtgccgc
    11101 tcgtgacatt gacctggctg cgggcatacg cctcgatgcg gtagtggcct acccgcccgg
    11161 cgttcagctt caggtactgg ttgtcgccat tgatatggct cgcgcgcagg tcgaagtagc
    11221 tgccgtcgcc gggccgcatg agcgacagtt cgagcctggc gagcacaccg tcgtcccagt
    11281 tgtcgtagcg cagccagttg gtgttgcgct cgtcgttgat gtcgaggtag ccaagcccga
    11341 acgtgccgtc gaacagccag ttgcccgacc cggtggtgcg cggctgcgag aattccggcg
    11401 ggcatagata caggtgcccg ctgggcgtgc gcttgcgtgc cgcggtgagc cagctcatgc
    11461 cgtcgggatc gcaggcgagt ggcgcgacgc cattcggctg gagcgagttg ccgaagtgca
    11521 ggtcgacgcc gacgccgctg tcctgggccg tggccaaccc cgcggcgcag acggcaagca
    11581 ccgcgaatcc cggggagata aaggagacac gccggaatcg ctgctgcatg accatacccc
    11641 cgcgctagcg atgcaacctg ggccccgatg ggtggttcga tccatgcacc tgtgcgtggc
    11701 aggtgatgca gccgcgcgcc atcagccgtt catcggcggc cgggccattc gcgagcgacg
    11761 tggttgtgag caggtcgttc gggtggcgca cgtgcgtgtg gcactgctgg cacaggaacg
    11821 ggatcggcgc cacgagcagc gcatgctggt tcgaaccgtg tggcgtgtgg cagttgaggc
    11881 agctgtcgcg cacgggggcg tgctcgaaca gcatcggccc gcgcttctcc gcatggcact
    11941 ggtagcaggt ctcgttgacc gagtcggtct tcatcagcga cgcgttgatc gtgccgtggg
    12001 ggttgtggca atcggcgcag ctcatctgcc cttcgggcag cggcatgtgc gagcggcgat
    12061 tgaactgctg gcgaatgtcc ttgtggcagg tggcgcaggt ttcgctgatc gactgccgcg
    12121 cgagcaggcc ctccgcggag aacttcgcca tcgggttgtg gcagtcgctg caggacagtt
    12181 cgttgcgctg gtggacggag ccgagccagt gatcgcgctg tccgccggag tggcagccga
    12241 ggcagccggc gctttgcgcg gccaccggtg tgccgccgtc gcgcgtgaac gcgatgatcg
    12301 tgccgggctt gcccggcgcc tggctgtgga cggagcccgc gccgtggcag gcctcgcagg
    12361 tcggtgtctt cgggtcggcg gccaacgctg cattcatgcc gagtgcgtgc agggtgtgca
    12421 ggaaatgccg gttttcctgc tggtggcacg cgacgcacat ctgctggccg accacggtgg
    12481 cctcgggatg cagcggattg actggcaccg aggcgtgggc aaagccgggc ggggcgctca
    12541 gccagcgtgc aatatcgttc ttgccgacgc cgagatccgt ctcgggaagg ttgtatggat
    12601 tcgatacggt agggttcgcc tgttgagcag cgaccggccc ggcaagccag cctgcaaaca
    12661 gcagcgccgc ggctgcaagg gacagcagcc cgagaaatgc tcgcattgcg cctccctggt
    12721 agaagtcggc ccaaatgcaa tcggaccgac tataaccccg ggcggcttgt gcgtacgggc
    12781 cgcatcttgc gatccatcaa tccgcgattt gcgcgcccgc cacgatcgcc gccgcggccg
    12841 cccgcgcctt cgcgcgcgcc gcctcgatcg actcgccgcg cgcgagtgcg acccccatgc
    12901 gccggtgacc ccgcacctcg ggtttgccga acaggcgcag cgccgtgtcg ggctcggcga
    12961 gcgcggcctc gacgccggcg tagcggatgt cgccgccatg gccctcgagc aggatcgccg
    13021 cggaggccga cggcccgtgc tgcgcgatgt tcgggatcgg caggccgagg atcgcacgca
    13081 catgcagcgc gaactgcgag agatcctggg cgatcatcgt caccatgccg gtgtcgtgcg
    13141 ggcgcggtga gacttcgctg aaccacacgt cgtcgccgcg cacgaacatc tcgacgccga
    13201 agatgccgcg cccgccgagc gcgtcggtca ccgtgcgcgc cgtgtgctgc gcgcgttcga
    13261 gcgcgaggcg gctcatagga tgcggctgcc aggactcgac gtagtcgccg tcgacctggc
    13321 ggtggccgat tggtgcgcag aaggcggtgc caccggcgtg gcgcaccgtg agcagggtga
    13381 tctcgtactc gaaatcgacg aagccctcga cgatcacgcg gccggcgccg gcgcgcccgc
    13441 ccgtctgcgc gtggcgccat gcggcttcga tctcctccgc cgtgcgcacg aggctctggc
    13501 ccttgccgga gctcgacatc acgggtttca ccacgcaggg caggccgatc gcggcgaccg
    13561 ccgcgcggta gtccgccggc gaatcggcga aacggtacgg cgaggtcgca aggcccagct
    13621 ccccggcagc aagctgccgg atgccctcgc ggttcatggt caggtgcgcg gcgcgcgcgc
    13681 acgggatcac cgtgcagccc tccgcctcga gcgcgacgag ctcggccgtg gcgatcgcct
    13741 cgatctcggg cacgacgaag gtgggtcgct cgcgttcgat gacggcgcgc ggggcccgcg
    13801 ggtcgagcat gtcgatcacg tggtgccggt gcgcgacctg catcgccggc gcgccggggt
    13861 agcgatcgac cgcgatcacc tcgacgccga gccgctgcgc ctcgatggtc acttccttgc
    13921 cgagttcacc ggcgccgagc agcagcaggc gggtcgccgc ctcggtgccg ggggtgccga
    13981 gggtggtcat gcgcagcatc cgttcgtcat ccggacagtt tgccggttag acttttggga
    14041 tgcacctgtc cgccgagcac gctgacggac gtctgaccct gcacctgctc ggtgaatggc
    14101 gggcgctcga cctgaccgag atccgagccg agctcgccgc gctgccgctc gagaaggtga
    14161 gcgagatcac catcgacacg ggcgcggtcg agcggctcga tctgtcgggc gcctggctgc
    14221 tgcgcgaatt cctcgacagg gccgaggcgg gcggcgcccg caaggtgggg ttcagcggcg
    14281 agcggccggc gcagctcgcg ctgatcgatc gcacccgggc cgaggacgcc gaggccgcac
    14341 gcgcgcggcc tccgatggag gaggccgagg aatcgcccgt cgccgcggtc gggcgcacca
    14401 cggtgatcca gtggcgcggc attcgcgacg gcctcgactt cctgggccgg atcaccgtgc
    14461 agctgctgcg cgcctttggc agcgtgaagc gcatgcgcct cacctcgatc acccgccacg
    14521 tgtacgacac cggcatcacc gcgatcccga tcgtctcgct gatcgccttc ctgatcagcg
    14581 tgatcatcgc ctacatgagt gcgcagcagc tgcgcaactt cggcgccgaa atctatgtcg
    14641 tcgacctggt cacggtcggc gtgctgcgcg aactgggcgt gctgctcacc gcgatcatcg
    14701 tcgcgggccg ttcgggcagc gccttcgccg ccgagatcgg tgcgatgaaa ctcaatgagg
    14761 aagtcgacgc actgaccgcg accggcatcg agccgatcga ggtgctggtg ctgccgcgcg
    14821 tgatcggcct gatgatcgcg ctgccgctgc tcaccgtgat cgccgacggt gtgggcatcg
    14881 ccggcggcgc gctgctctgc aaaatactgc tcgacatgcc gctcgtgcag tacttcgcgc
    14941 gcgtcgagga cgcgatcgcg tcgaccacct tctgggccgg cctcgtcaag gcgccggtgt
    15001 tcgcgatcct gatcgcgacg gccggcacgt atcgcggcct gcaggtgcgc ggctcgtcgc
    15061 gcgaactcgg ccggctcacg accttcgcgg tcgtgcagtc gatcttcctc gtgatcctcg
    15121 ccgacgcgtt gttcgcggtg ctgttcatgg agatcgactt ctgatggccg cgatcgtcga
    15181 ggtgcgcgac atcgtcaatc gcttcggcac ccaggtcgtg cacgacggcc tcgacatgca
    15241 ggtgctcgag ggcgaggtgt tcggcatcgt cggcggctcg ggttcgggca agtcggtgct
    15301 gctgcggacg atcctcggcc tgcgcaagcc ccagcgcggc gtggtgctgc tcgaagggca
    15361 ggacatcacg caccagtcgt ggaacgagct gcgcgcggcg aaggcgggct acggcgtcac
    15421 tttccagcag ggcgcgcttt acagctcgct cacggtgctg cagaacatcc agctgccgat
    15481 gatcgagcac ctgcgactgc cgccggacgc gctcgatgcg ctggcgaagc tgaaactgcg
    15541 catcgtgggg ctcgcggagg aggcgggatc caagtacccc gcgcagctct cgggcggcat
    15601 ggtcaagcgc gcggccctcg cgcgcgccct ggcactcgat ccgaagctgc tgttcctcga
    15661 cgagcccacc gcgggcctcg acccgatcag cgcggccgcg ttcgacgagc tgctgatggc
    15721 gctgcagcgc cagctcgcac tgaccgtggt gatgatcacc cacgatctgg acacgatctt
    15781 ccgaacctgc aatcgtgtcg gcgtgatagt cgaccgccac atgatcagcg ggaccctgga
    15841 ggagatcgtc gacaatccgc accagtggat tcgcgagtat ttccacggcg agcgcgcatt
    15901 gcgattcggg gagcgccatg gaacgtgagg cgaactacac ggccgtcggc gcctttgtgc
    15961 tgctgatcag cgcgatggcg gtgctgttcg tctactggta ctcggacggt gtcgagcggc
    16021 gcgactacac ccgctacgag atctatttcg acggcagcgt gagcggattg tcgcggggct
    16081 cgcccgtgcg ctacctcggc gtcgacgtcg ggcgggtcgc ggtgatgcgc atcgaccggc
    16141 gctcgagctc gcgggtgcag gtgatcgcgg acatcgattc ggaggcgccg gtgtcggccc
    16201 gcaccgtcgc gcagctgtcg ctgcagggcg tgaccggcct gctgtatatc gacctgatcg
    16261 agaatcgcgg caacctcaag gtgatcgatc cggtgccgag cgagcgctat ccggtgatcg
    16321 cctcgtcgcg ctcgagcttc gacctgttcc tcgcctcgct tcccgaactg gtcgccaagg
    16381 cgaacgacac gctgatccgc gtcaactcga tcctgtccga ccagaacatc gactcgctgc
    16441 agaccacgct taagagcatc gagcgggccg ccgggcactt tccgggcatg atgcgcgacg
    16501 ccgaatcgct ggtcgccgaa ctgcgcagct cctcgcgcga cttcagcgcg acgatggtcg
    16561 tg
//
LOCUS       DAOQVY010000048        16384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_4026_length_16384_cov_5.444608, whole genome shotgun sequence.
ACCESSION   DAOQVY010000048 DAOQVY010000000
VERSION     DAOQVY010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16384
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4026_length_16384_cov_5.444608"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PKN91_06285"
     CDS             complement(<1..769)
                     /locus_tag="PKN91_06285"
                     /inference="COORDINATES: protein motif:HMM:NF018716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HNS27714.1"
                     /translation="MREHLWLVAVMAAGIGAAPAWAAVTAEEAARLGADLTPLGGEKQ
                     GNADGTIPAWEGGLDTPAKAGVPNYRIGDRRPDPFASERPLFTITPANMSQYASRLTE
                     GQKKLLQQYSSTYKMPVYPTHRTAAVPQRIYDATKRIATTANLAANGNGVTGAGEGIP
                     FPIPKEGVEVFWNHILRYRANAVLRNIGQAPVTASGAYTLVKFRDDTFAAYSMPGATS
                     DNLNNIILYFIQETVAPARLAGEILLVHETLDQSKENR"
     gene            complement(797..2884)
                     /locus_tag="PKN91_06290"
     CDS             complement(797..2884)
                     /locus_tag="PKN91_06290"
                     /inference="COORDINATES: protein motif:HMM:NF018662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HNS27715.1"
                     /translation="MDSTSGARGRLLAAAVSCAVAAPAALAFEFQNESGTLRGSWDTT
                     LSYGQAWRIEDRDCRLIASANGGCGRSANGDDGDLNYGHGLFSRAAKIVSEIGVDYQD
                     VGLFVRGAALYDFDVMDGNTARTPLSRAAEGLVGSYERLLDALGYWKFGEGAMQGEFR
                     LGRQVASWGESTYIQGGINIINHFDVAALRVPGAELREGFLPQEMALLSLQFNEYLSG
                     QAVYIADWDETIPEPVGSYFSTNDIAVTGGRTVLLGFGAFSDQGVDFSALGGPLIADF
                     QQVPRLATREPDDQGQFGLNLKLYLPNFNNGTEFGLYFINYHSRVPVISGRTGTQAGI
                     GNSIGGITAVGAAAQGLAAGLPVQAAIATAAQVAVQRAAAAGGNLSLATATQYATIGA
                     NTHLVGGNVTAQATNIGTHEYARTAGYFTEYPEDIQLIGLSFNTQLQRTGVALQGEVT
                     YRMDQPLQYDDVELLFAALTPFEQAAFAATQPPGTPFPSACLPGAGATLSRCGQLGAY
                     PLNTEIRGWGTHDVGQFQVTATKSFANVLRASQLVIVGEVGVTHIFDLEDKLTGGPNG
                     RGLRYNGPATSVSGNAELAGRHFNEVEPLDKFADDTAWGYRLAGRLEYPGLVGPWNVL
                     PRLAFQHDVSGISPGPGGNFQEGRYGLTLGVAANLRAMWELDFAWTKFGGAGRWNELN
                     DRDYLSATIKVSF"
     gene            complement(2977..5412)
                     /gene="plsB"
                     /locus_tag="PKN91_06295"
     CDS             complement(2977..5412)
                     /gene="plsB"
                     /locus_tag="PKN91_06295"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF003441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HNS27716.1"
                     /translation="MEWLLRRLLSLWVRATVRPEDIAARLTAQPAPVCYLLERASSTD
                     LAVLQNVCARGGLPRPGARLGSGELRRFRSWFYLSRPLNFWSARVDRRPPAVLREMLE
                     VLQRNPAFDVDLVPTAVYWGRAPQKEDSWLRMLLSEDWALATRLRKALTVVFNGRATM
                     VEFGEPVSLRSLLTEGDRDPNLQARRIARVARAQFRRQRAARIGPDLSHRRTIVTAVL
                     RTRAVRAAAALEMRERQVTRREALETAQKYAEEIAANYSHRFVTVMEALLRRLWTRLY
                     DGVTFGHIETLRQVAEGNEIVYVSCHRSHMDYLLLSYAIYVQGYAIPHIAAGINLNMP
                     VVGRFLRKGGAFFIRRSFRGNALYTVVFMKYLATIMARGHSIEFFIEGGRSRTGRLLR
                     PKTGMLAMTIRSYLREPVRPIVFVPVYFGYERVVEAATYVSELSGKPKEKESVLGMLK
                     ALRVLREKFGRVYVNLGEPIALDAVLARIAPDWRAQGFEHEARAPWISHAVDEVAGQL
                     MRNINSAAAVTPVNLLALILLATPHQAMLRDDLLRQVELYLALLRAVPYDPRVTVTDE
                     SPEAILAYGEAMKMVTLQPHKLGDIVRMSDESAVLSAWYRNNVLHLFAMPSLIACAFV
                     GNSIMRTEDIHRLVWRIYPYIAAELFIRWPEEEVGAVTDALLAALAARGLITATADGG
                     GWRRAPSTTPEAVQLSFLAQATIQTVERYYLAIGVLISAGSGRITQAALAERCQLMAQ
                     RMTMLHGFNSPEFFDRNLFGDFLDRLRERGVIRVDNGGLLAFDEVLERVAQDAEFVLS
                     EQIRHSILQVVHA"
     gene            complement(5433..5951)
                     /locus_tag="PKN91_06300"
     CDS             complement(5433..5951)
                     /locus_tag="PKN91_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HNS27717.1"
                     /translation="MRRTVVFSHGKDSSPHSRKIIALTETARGEGYEVLAVDYRGIDS
                     PKARVTHLVDSCKDVQGDLVLVGSSLGGYVSLVAAPTLHARGVFLMAPAIYFDELPPL
                     RERSIDCPFTVVHGWRDDVVPIEDSLRLAREYRATLHIVDSDHQLHSHLRMIRFYFEH
                     FLIGLDLPRDLQ"
     gene            complement(5973..6317)
                     /locus_tag="PKN91_06305"
     CDS             complement(5973..6317)
                     /locus_tag="PKN91_06305"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNS27718.1"
                     /translation="MDERQQAMASVWRVVRAIPRGATLSYGEVARQAGLPRRARWVAV
                     ALKAAPSQLRLPWHRVVGAGGRILFAPGSRLQREQARLLRAEGRELRGNRVLAVARGD
                     RKSALDAWLWKR"
     gene            6353..9940
                     /locus_tag="PKN91_06310"
     CDS             6353..9940
                     /locus_tag="PKN91_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HNS27719.1"
                     /translation="MDRWEFWIDRGGTFTDVIGRSPEGRLHVRKVLSQPVGDLDPGLA
                     AAGAILAADRRGAARRPAARISAFRIGTTVATNALLERRGEPVVLVTTRGFRDALRIG
                     YQHRPDVFARQIVLPAPLYAEVIEAGERIGAHGELIEPLDAAALAAELAAARARGRRS
                     VAIVFMHGYRYPQHEAQAADIARAAGFETVTASHAASPLVRFVARGDTTVFDAYLAPP
                     LEEYVGRVAAAARRLDPDAQLLLMQSSGGLVDAQRVRAAASVLSGPAGGLTGMARIGE
                     RLALDALIGFDMGGTSTDVSIYAGRLAQRFEHEIAGTRLQAPMLDIHTIAAGGGSVLG
                     FADGRMQVGPASAGSHPGPACYGRGGPLTVTDLQVALGRLQPAFLPAVFGPGGDAPID
                     PAIVLTTLHRQAQAINAAQGRDVPDEDIAAAWLEVAVESMANAVRQVCAAQGEDPSRF
                     ALFCFGGAAPQHACAVAAACDMRRVVIHPLASVLSAFGIGVADWLETRRRSLRMELDA
                     AGLAAARAELETLARSAVAALPTGYPVSVRRLLEVRAGESEVTFDIESDAASPAAAFY
                     AAQQARYGFAERDAELVIESIRVEARASPPFPALHDVVATPLASLPRRVRTRFGGAWH
                     DTPVLSWADFTAAAGPVLVVDANFTVVVEPGWHARRAPGGELLLEREAQVPAPRHAAA
                     APDPARIEIFGNLFMHIATQMGSVLRDTAQSINIKERLDYSCALFDGEGRLLANAPHM
                     PVHLGSMGASVREVIRRHGAGLRPGDAWLLNSPYHGGTHLPDITVVTPAFLAGGARPT
                     AFVASRAHHADIGGITPGSMPPFSTHIDEEGVLLEGLRIVAQGAFDEAALRAALARAP
                     HPARNPGQNVADVRAQLAANARGIHELERAARRHGSATLLAHFDAVRANAEECVRAAI
                     SRLSTGEFRCELDGGQRIAVRIEVDAARRSARIDFTGSSPQGAHNFNAPRAVCVAAVI
                     YVFRTLIDRPIPLNEGCLAPLELVIPPGSMLAPEAPAAVVAGNVETSQVLVDALYGAL
                     GMLAASQGTMNNLTFGDATLQYYETIAGGAGAGPDFPGASATQTHMTNSRLTDPEILE
                     LRYPVRVREFSLRTGSGGAGRQRGGDGVVRAIEFLAPMSGAMLANRRRTRPFGLRGGE
                     PGAAGITRLRRAAGGIIELAAADRFDAQPGDVIEVLTPGGGGFGPPSRT"
     gene            complement(9937..11562)
                     /locus_tag="PKN91_06315"
     CDS             complement(9937..11562)
                     /locus_tag="PKN91_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27720.1"
                     /translation="MADAVTGFDISEQSGPGHAIARVATGITAFVGRALKGPVNNPVV
                     IHNFAEYVRLFGGLWQPSTMSYALEQYFENGGRTAIVVRVANSARPPTITLPTGGAPL
                     TLVGIAPGSREYLRASVDLDGIGENEEDRFNLVVQRVRTPGSERIEDQEIYRRLSVLP
                     GSDRYVATVLLDSQLVRVLGEVPPAQRPLRTPSRTSSLMAGYVASNPDGDDGAALTDY
                     DIIGSSQRGTGLFSLRFAPRFDLLCVPPLTRTDDVGLATLMVASRLCRERHAMLIVDP
                     PASWTSAAGAVESLRPWPFRSEQALMYFPRVVAFDRVRGRHEVFASCGAAAGLIARGD
                     AATPVWSAGENDEPVLRPALRPATQIDDVERGRLAQLGVNTFATVRPLHRTGSPRTLG
                     AGGSGPADWTYLAARRLALFILASVEEGTRWIVFQHNQPAAWARLRNQVAAFLAEIEE
                     EGAFVGRTAAERWFVICDERLNPPESVAAGKVHLLLGFAPMRSGDFHAFLVTHQAGSS
                     SVRPVSVNRLATSGEQVEEEIETAILRGIALEA"
     gene            complement(11671..11835)
                     /locus_tag="PKN91_06320"
     CDS             complement(11671..11835)
                     /locus_tag="PKN91_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27721.1"
                     /translation="MSDTQPQAPGGQRLSEWLKLMLDEIERKRRDLDAANAEQRRRAA
                     GQDSPTESRR"
     gene            complement(11832..13739)
                     /locus_tag="PKN91_06325"
     CDS             complement(11832..13739)
                     /locus_tag="PKN91_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNS27722.1"
                     /translation="MRGLAMRPMIRVVAAASLALLASCAARPVADSAAAPLQSGRDYH
                     TLANVDEFRVRHLALDLTVDFDSKRLAGGAELTVERIAGTGRTLVLDTRGLDVAAVEW
                     LAPGAAPQPLAFGVGAEVEHLGAPLAITLPGDLARFTIRIRYATRPDASGLQWVEPAQ
                     TAGKVAPFLYSQSQAIHARSWVPVQDSPQVRFTFEATLRVPAGLRALMGAEPVGGPGT
                     DGVFRFDMPQPIPSYLMAIAAGRLEFQPIGSRTGVYAEPEIIAAAAREFADTEKMLET
                     GESLFGPYRWGRYDVLILPPSFPFGGMENPRLSFITPTVIAGDRSLVALIAHELAHSW
                     SGNLVTNATWRDFWLNEGFTVYLESRIMAAVYGPRRAAMEDLLGFQSLERELATLPPA
                     EQVLAIDLRGRDPDDGVTDVPYEKGRFFLRWLESRVGAAPLHAFLQDYFAHFAFQSIT
                     VEQFLGYLDSKLLAQHPDSVTAAELDEWIHQPGLPASAIRPASDALVQVDAARAAFLA
                     GTRAATDLPHAGWSTQEWLYFLANLPQQMPRAQLESLDAAFDFTQATNNEIAFNWLQI
                     AIRNDYEPAWPRLEQYLESIGRRKLIVPLYTAMMKTQSGAQRARAVYARARPGYHPIT
                     AASLDAIVGAP"
     gene            complement(13739..14965)
                     /locus_tag="PKN91_06330"
     CDS             complement(13739..14965)
                     /locus_tag="PKN91_06330"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNS27723.1"
                     /translation="MSRQHRTTAFLRASACVLTLAALAGCSLLEGSGFGRKAPAEPEA
                     PPEPQYAPPVATSRFEIGPNDDVVGVLQKTVVGADDTFSDIARRFNIGYEEMVRANPG
                     VDPWLPGAGREVVVPTRFILPNAPRQGIVINLAAMRIYYYEPRKGDAPQVVHTHPIGV
                     GRVGWATPEGTTRIVRKQKDPVWRPTPSILKEHAENGDPLPAVVPAGPDNPLGNRAMY
                     LGWPTYLVHGTNKPYGVGIRSSHGCIRLYPEDIEPLFEKVAVGTPVRVVNQPFVFGWA
                     EDKLFLQAYDVLEEDTRDFAKAPKKLLSKSLATRIQKQLKERGESVDWDAVAAIAHAP
                     RGIPAPIAGGVLSIESEIEVAQVVENRVPDGATWDGVSGLPIDEATFQEIFSDNQDVA
                     PAPATGANAPGRKPKG"
     gene            complement(14996..15493)
                     /locus_tag="PKN91_06335"
     CDS             complement(14996..15493)
                     /locus_tag="PKN91_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNS27724.1"
                     /translation="MSLARWLRHLFASHWMTRRRFDRLACATIERAVGEVESRHAGEI
                     RVAVETSLDLADLWFGVTARQRALAVFGMLGVWDTANNNGVLLYVLMADHDVEIVADR
                     GIASRVPRAEWAAICRHIETAFRAGRHGEGLADGVRAVGEVLSRHFPHEGGDHDELRN
                     APVLL"
     gene            complement(15493..16374)
                     /locus_tag="PKN91_06340"
     CDS             complement(15493..16374)
                     /locus_tag="PKN91_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HNS27725.1"
                     /translation="MLAGVTLALAALAAGAPAPAQDLLPVPPLTARVTDTTGTLDSAQ
                     RAALEAKLEALEREKGAQVAVLLVPTTGPEDIAQYSIRVADAWKLGRAQPDDGVLLLV
                     AKDDRRLRIEVGYGLEGALPDAIASRIIRETITPHFRQNDYYGGIDAGVDQILAVVRG
                     EELPPPDERWQGTDAVSRGLDLLPFLLFGVLFASGILRSIFGRGIGSLLTGGVTGGLV
                     YLVASALGIAIAVGIGAWLLSLLLASLGGGNWSSGPRHGGWGGGWGGSSGGGGFGGGG
                     FRGGGGGFGGGGASGRW"
BASE COUNT         2446 a         5751 c         5751 g         2436 t
ORIGIN      
        1 tgcggttctc cttcgactgg tcgagcgttt cgtgcaccag caggatctcg ccggcgagcc
       61 gcgccggagc gaccgtctcc tggatgaagt acaggatgat gttgttcagg ttgtcggacg
      121 tggcgcccgg catgctgtag gcggcgaagg tgtcgtcacg gaacttcacc agcgtgtagg
      181 cgcccgacgc cgtgaccggc gcctggccga tgttgcgcag gaccgcattc gcgcgatagc
      241 gcaggatgtg gttccagaac acctcgacgc cttccttcgg gatcgggaac gggatgccct
      301 cgcccgcgcc ggtgacgccg ttgccgttcg cggcgaggtt cgcggtggtc gcgatgcgct
      361 tcgtcgcatc gtagatgcgc tgggggaccg ccgccgtgcg atgggtcggg tagaccggca
      421 tcttgtaggt ggacgagtac tgctgcagca gcttcttctg cccctcggtc aggcgggacg
      481 cgtactgcga catgttggcc ggcgtgatcg tgaacagcgg ccgttcgctc gcgaagggat
      541 cgggacggcg atcgccgatc cggtagttgg gcacgccggc cttcgcggga gtgtcgagac
      601 cgccctccca tgccgggatc gtgccgtcgg cgttgccctg cttctcgccg ccgagcggcg
      661 tgagatcagc gccgagccgc gcggcctcct cggcggtcac cgcagcccag gctggtgccg
      721 cgccaatgcc ggcggccatg acagcgacca gccacaagtg ctctctcatg ccaccctcct
      781 agtgcgcggc gcacgttcag aacgagacct tgatcgtggc cgacaggtag tcgcgatcat
      841 tgagctcgtt ccagcggccc gcgcctccga acttcgtcca tgcgaaatcg agttcccaca
      901 tcgcgcgcag gtttgcggcg acgcccagcg tgagcccgta gcgcccctcc tggaagttgc
      961 cgccgggccc cggcgaaatg ccgctcacat cgtgttggaa cgcgagccgg ggcaggacgt
     1021 tccatggacc gacgagaccc gggtactcca gccggccggc gagacgatag ccccatgccg
     1081 tgtcgtccgc gaacttgtcc agtggttcga cttcgttgaa gtggcggccg gcaagttccg
     1141 cgttgccgct gaccgaggtg gccggaccgt tgtagcgcag gccgcggccg ttcggcccgc
     1201 cggtgagttt gtcttcgagg tcgaagatgt gggtgacgcc cacttcgccg acgatcacga
     1261 gctgcgaggc gcgcagcaca ttggcgaagg acttggtcgc ggtcacctgg aactggccga
     1321 cgtcgtgcgt cccccagccc cggatctccg tattcagcgg ataggcgccg agctgtccgc
     1381 agcggctcag agtggctccg gcgccgggca ggcaggcgct cgggaacggc gtgccgggcg
     1441 gctgcgtggc cgcaaaggcc gcctgctcga acggcgtgag tgccgcgaac agcagctcga
     1501 cgtcgtcgta ctgcagcggc tggtccatcc tgtaggtcac ttcgccctgc agcgcgacgc
     1561 cggtgcgctg cagctgcgtg ttgaacgaca ggccgatcag ctggatgtcc tcgggatact
     1621 cggtgaaata gccggcggtg cgcgcgtact cgtgcgtacc gatgttggtg gcctgcgcag
     1681 tgacattgcc gccgacgagg tgggtgttgg cgccgatcgt ggcgtactgg gtcgccgtcg
     1741 cgagcgacag gttgccgccc gccgccgcgg cgcgctgcac ggcgacctgc gcggcggtcg
     1801 cgatcgccgc ctgcaccggc aggcccgccg cgagtccctg cgcggctgcg ccgaccgcgg
     1861 taatgccgcc gatcgagttg ccgatgcccg cctgggtgcc ggtgcggccg gagatcaccg
     1921 gcacgcgact gtggtaattg atgaagtaca ggccgaactc ggtgccgttg ttgaagttcg
     1981 gcaggtagag cttgaggttc aagccgaact ggccctggtc gtcgggctcg cgggtcgcga
     2041 ggcgcggcac ctgctggaaa tcggcgatca gcgggcctcc cagcgccgag aagtcgacac
     2101 cctggtcgga gaacgcgccg aagccgagca gcacggtgcg cccgccggtg accgcgatgt
     2161 cgttggtgct gaagtaggac cccaccggct cgggaatcgt ctcgtcccag tcggcgatgt
     2221 agaccgcctg accgctcagg tattcgttga actgcagcga cagcagcgcc atctcctgcg
     2281 gcaggaagcc ttcgcgcagt tcggcgccgg gaacgcgcag cgccgcgacg tcgaagtgat
     2341 tgatgatgtt gatgccgccc tggatatagg tgctctcgcc ccagctcgcg acctggcggc
     2401 cgaggcggaa ctcgccctgc atggctcctt cgccgaactt ccagtagccg agggcgtcga
     2461 gcaggcgctc gtagctgccg acgagaccct cggccgcgcg cgacagcggc gtgcgcgcgg
     2521 tgttgccgtc catgacgtcg aagtcgtaca gcgccgcacc gcgcacgaac agcccgacgt
     2581 cctggtagtc gacgccgatc tccgagacga tcttcgccgc gcggctgaac aggccatggc
     2641 cgtagttcag gtcgccgtca tcgccgttgg cggagcgccc gcagccgccg ttggcgctcg
     2701 cgatcagccg gcagtcgcga tcctcgatgc gccaggcctg gccgtagctg agcgtggtgt
     2761 cccagctgcc gcgcagcgtg ccggattcgt tctggaactc gaatgccagt gccgcgggcg
     2821 cggcgacggc gcatgaaact gccgccgcca gcagccggcc gcgggctccg ctggtgctgt
     2881 ccatgtcgct ccccacgctg ccgtcagcat gcgaacggtt gatagggtgt cacgcgtgga
     2941 acctcgcgcg taccgtcgat aaacatagca cgcacctcac gcatgcacca cttgcaggat
     3001 gctgtgacgg atctgctcgg acagcacgaa ctccgcgtcc tgcgcgacgc gctccaggac
     3061 ctcgtcgaac gcgagcagac cgccgttgtc gacgcggatc acgccgcgct cgcgcaggcg
     3121 gtcgaggaaa tcgccgaaca ggttgcggtc gaagaactcc ggcgagttga agccatgcag
     3181 catcgtcatg cgctgcgcca tcagctggca gcgctccgcg agcgcggcct gggtgatgcg
     3241 gccactcccc gcgctgatca gcacgccgat cgcgaggtag tagcgctcga ccgtctggat
     3301 cgttgcctgg gcgaggaacg agagctgcac ggcttcgggc gtcgtcgatg gcgcccgccg
     3361 ccagccgccg ccgtctgcgg tcgcggtgat gagcccgcgg gccgcgagcg ccgcgagcag
     3421 cgcatcggtg acggccccca cctcctcttc gggccagcgg atgaacagct ccgcggcgat
     3481 gtacggatag atgcgccaga cgagccggtg gatgtcctcg gtgcgcatga tcgaattgcc
     3541 gacgaaggcg caggcgatca gcgacggcat cgcgaacagg tgcagcacgt tgttgcggta
     3601 ccacgccgac agcaccgcgc tctcgtcgct catgcgcacg atgtcgccga gcttgtgggg
     3661 ttgcagcgtg accatcttca tcgcctcgcc gtaggcgagg atcgcctcag gcgattcatc
     3721 ggtcacggtg acgcgcgggt cgtagggcac ggcccgcagc agcgcgagat acagttcgac
     3781 ctggcgcagc aggtcgtcgc gcagcattgc ctggtgcggc gtggcgagca ggatcagcgc
     3841 gagcaggttg accggagtga ccgcggccgc cgagttgatg ttgcgcatca gctggccggc
     3901 aacctcgtcg acggcgtgcg agatccacgg cgcgcgcgcc tcgtgctcga aaccctgcgc
     3961 gcgccagtcc ggcgcgatcc gcgcgagcac ggcgtcgagc gcgatcggct cgccgagatt
     4021 gacatacacg cgaccgaact tctcgcgcag cacgcgcagc gccttcagca tgccgaggac
     4081 gctctccttt tccttcggct tgcccgacag ctcgctgaca taggtcgcgg cctcgaccac
     4141 ccgctcgtag ccgaagtaca cgggcacgaa cacgatcggc cgcaccggct cgcgcaggta
     4201 gctgcgtatg gtcatcgcga gcatgccggt cttcggccgc agcagccgcc cggtgcgcga
     4261 gcgaccgcct tcgatgaaga attcgatcga gtgcccgcgc gccatgatcg tcgcgagata
     4321 cttcatgaac acgaccgtgt agagcgcgtt gcccctgaag ctgcggcgga tgaagaacgc
     4381 cccgcccttc ctgaggaaac gcccgaccac cggcatgttg aggttgatgc ccgccgcgat
     4441 gtgcggaatc gcgtagccct gcacgtagat cgcgtaggac agcagcaggt agtccatgtg
     4501 gctgcggtgg caggacacgt acacgatctc gttgccctcg gccacctgcc ggagcgtctc
     4561 gatgtggccg aaggtcacgc cgtcgtagag ccgggtccac aggcgacgca gcagcgcctc
     4621 catgaccgtg acgaagcggt gcgaatagtt cgccgcgatc tcctcggcgt atttctgcgc
     4681 ggtctcgagc gcctcgcgcc gggtcacctg ccgctcgcgc atctcgagtg cggcggccgc
     4741 gcgcaccgcc cgcgtgcgca gcaccgccgt gacgatcgtg cgccgatgcg acaggtcggg
     4801 cccgatgcgc gccgcacgct ggcggcgaaa ctgcgcgcgc gcgacgcgcg cgatgcgccg
     4861 cgcctgcagg ttggggtcgc ggtcgccctc ggtgagcagg ctgcgcagcg ataccggctc
     4921 gccgaactcg accatcgtcg cgcggccatt gaagaccacg gtcagcgcct tgcgcaggcg
     4981 ggtggcgagc gcccagtcct cggaaagcag catgcgcagc caggagtctt ccttctgcgg
     5041 cgcacggccc cagtacaccg cggtcggcac gagatcgacg tcgaacgcgg gattgcgctg
     5101 caggacctcg agcatctcgc gcaacacggc gggcgggcgc cggtcgacgc gcgcactcca
     5161 gaaattcagc ggccgcgaaa gatagaacca ggaacgaaaa cgcctgagtt cgccgctgcc
     5221 gaggcgcgca cccggccgcg gcaggccgcc gcgcgcgcac acgttctgca gtaccgcgag
     5281 gtcggtcgag ctggcgcgct cgagcaggta gcacaccggc gcgggctgcg cggtcagccg
     5341 ggcagcgatg tcctccggtc tgacagtcgc cctgacccag agcgacagca ggcgcctcag
     5401 cagccattcc atggggtatc agccgccgcg tgtcattgca ggtcgcgcgg cagatcgaga
     5461 ccgatcagga aatgctcgaa gtagaagcgg atcatccgca ggtgggagtg cagctgatgg
     5521 tcgctgtcca cgatatgcag cgtggcgcga tactcgcgcg cgaggcgcag gctgtcctcg
     5581 atgggcacga cgtcgtcgcg ccacccgtgc acgaccgtga acgggcagtc gatcgagcgc
     5641 tcgcgcagcg gcggcaactc gtcgaaatag atggccggcg ccatcaggaa caccccgcgc
     5701 gcgtgcaggg tcggcgccgc aaccagcgat acatagccgc cgaggctcga gccgaccagc
     5761 acgaggtcgc cctgtacatc cttgcaggaa tcgacgaggt gggtgacgcg cgccttgggc
     5821 gaatcgatgc cccgatagtc gaccgccagc acttcatagc cctcgccgcg ggcggtttcg
     5881 gtgagcgcaa tgatcttgcg actgtgcggg gaagagtcct tcccgtgtga aaacacgact
     5941 gtacggcgca tccaaagcat tgtaacccct tatcagcgct tccagagcca ggcgtcaagt
     6001 gcggatttgc gatccccgcg cgcgacggca agcacgcgat tgccacgcag ctcgcggccc
     6061 tcggcgcgca gcagccgggc ctgctcgcgt tgcaggcgcg aacccggcgc aaacagaatg
     6121 cgaccgcccg cgccgaccac ccggtgccag ggcaggcgca gctgcgaggg cgcggccttg
     6181 agcgcgacgg cgacccagcg cgcgcgccgc ggcagtcccg cctggcgcgc gacttcgcca
     6241 tagctcagtg tcgcgccgcg cggaatcgcg cgcacgacgc gccatacgct cgccatggcc
     6301 tgctgccgct catccataat gcccgcctcg catgccgcaa ttgtaggagc tcgtggaccg
     6361 ctgggaattc tggatcgacc gcggcggcac tttcaccgat gtgatcggcc gctcgcccga
     6421 gggccgcctg cacgtgcgca aggtgctctc gcaacccgtc ggcgatctcg acccgggcct
     6481 cgccgcggcc ggggcgatcc tcgcagcgga ccgccgcggc gcggcgcggc ggccggcggc
     6541 gcgcatctcg gcgttcagga tcggcacgac cgttgcgacc aatgccctgc tcgagcgccg
     6601 cggcgagccc gtggtgctcg tcacgacgcg cggctttcgc gatgcgttgc gcatcggcta
     6661 ccagcaccgc cccgacgtat tcgcgcggca gatcgtgttg cccgcgccgc tatatgccga
     6721 agtcatcgag gccggcgagc gcatcggtgc gcacggcgag ttgatcgagc cgctcgacgc
     6781 cgcggccctc gccgccgaac tcgcggcggc gagggcgcgc gggcgccgtt cggtcgccat
     6841 cgtgttcatg cacggctacc gctatccgca gcacgaggcg caggcggcgg acatcgcgcg
     6901 cgccgcgggc ttcgagacgg tgaccgcgtc gcatgccgcc tcgccgctcg tgcgcttcgt
     6961 cgcccgcggc gacaccaccg tgttcgacgc ctatctcgcc ccgccactcg aggaatatgt
     7021 cgggcgtgtc gccgccgcgg cgcgtcggct cgacccggac gcgcagctgc tgctgatgca
     7081 gagctcgggc ggcctcgtcg acgcgcagcg ggtgcgcgcg gcggcgagcg tgctgtcggg
     7141 accggccggc ggcctcaccg gcatggcccg gatcggcgag cgcctcgcgc tcgatgcgct
     7201 gatcggcttc gacatgggcg gcacctcgac cgatgtgtcg atctacgccg gccggctcgc
     7261 gcagcgcttc gagcacgaga tcgccggcac gcgcctgcag gcgccgatgc tcgacatcca
     7321 cacgatcgcc gcgggcggcg gctcggtgct cggcttcgcc gacggtcgca tgcaggtggg
     7381 ccccgcctcc gcgggctcgc atccgggccc ggcctgctac ggccgcggcg ggccgctcac
     7441 ggtaaccgac ctgcaggtcg cgctcgggcg cctgcagccg gccttcctgc ccgccgtgtt
     7501 cggcccgggc ggcgacgcgc ccatcgatcc ggccatcgtg ctgaccacgt tgcaccgaca
     7561 ggctcaggcg atcaatgccg cgcagggccg ggacgtgccc gatgaggaca tcgccgccgc
     7621 gtggctcgag gtcgccgtcg aatcaatggc gaacgccgtg cggcaggtgt gcgccgccca
     7681 gggcgaggat ccgtcgcgct ttgcgctctt ctgcttcggc ggcgccgcgc cccagcatgc
     7741 ctgcgcggtg gcggcggcct gcgacatgcg gcgcgtggtc attcatccgc tcgcgagcgt
     7801 gctttccgcg ttcggcatcg gggtcgccga ctggctcgag acccggcggc gcagcctgcg
     7861 catggagctc gatgccgcag gtctcgcggc ggcgcgcgcc gaactcgaga ctctggcgcg
     7921 gtctgccgtc gccgcgctgc cgaccggcta tccggtcagc gtgcgacggc tgctggaagt
     7981 gcgcgccggc gagagcgaag tgacgttcga catcgagagc gatgcggcct cgccggccgc
     8041 agcgttctac gccgcgcagc aggcgcgcta cggcttcgcc gagcgcgatg ccgaactcgt
     8101 catcgagtcg atccgcgtcg aagcgcgcgc ctcgccgccc ttccctgcgc tgcacgatgt
     8161 ggtggcaacg ccgctcgcct cgcttccccg gcgcgtgcgc acgcgctttg gcggcgcgtg
     8221 gcacgacacg ccagtgctgt cctgggcgga cttcacggcc gccgccgggc ctgtcctcgt
     8281 cgtcgatgca aacttcacgg tggtcgtcga acccggctgg cacgcgcgcc gcgcgcccgg
     8341 cggcgagctg ctgctcgagc gcgaggcgca ggtcccggcc ccgcggcacg cggccgccgc
     8401 acccgacccc gcgcgcatcg agatcttcgg caatctgttc atgcacatcg cgacgcagat
     8461 gggcagcgtg ctgcgagaca ccgcgcagtc gatcaacatc aaggagcgtc tcgactactc
     8521 ctgtgcgctg ttcgacggcg aaggccggct gcttgccaac gcgccgcaca tgccggtgca
     8581 cctcggctcg atgggcgctt cggtgcgcga ggtcatccgc cgccacggcg cgggactgcg
     8641 gccgggcgat gcgtggctgc tgaacagccc ctaccacggc ggcacgcacc tgcccgacat
     8701 caccgtcgtc acccccgcct tcctcgccgg cggcgcgcgg cccacggcat tcgtcgcctc
     8761 gcgcgcgcac cacgccgaca tcggtggcat cacgcccggc tcgatgccgc ccttcagcac
     8821 ccacatcgac gaggaaggcg tgctgctcga aggactgcgc atcgtcgcgc aaggcgcgtt
     8881 cgacgaggcc gcgctgcgcg cggcgctggc gcgtgcgccc catccggcgc gcaatcccgg
     8941 ccagaatgtc gccgacgtgc gcgcgcagct cgccgccaat gcgcgcggca tccatgagct
     9001 cgagcgcgcc gcgcggcgcc acggcagcgc gacgctgctc gcgcacttcg atgccgtgcg
     9061 cgccaatgcc gaggaatgcg tgcgcgcggc gatctcgcgc ctgtcgacgg gtgagttccg
     9121 ctgcgagctc gacggcgggc agcgcatcgc cgtgcgcatc gaagtcgacg ccgcgcgccg
     9181 cagtgcccgc atcgatttca ccggcagctc gccgcagggc gcccacaatt tcaacgcgcc
     9241 gcgcgcggtg tgcgtcgccg ccgtgatcta cgtgttccgc acattgatcg accggccgat
     9301 accgctcaac gagggctgcc tcgcgccgct cgaactcgtg atcccgcccg gctcgatgct
     9361 cgctcccgag gcgccggcgg cggtggtcgc cggcaatgtc gagacctcgc aggtgctggt
     9421 cgatgcgctg tacggcgcgc tcggcatgct ggccgcatca cagggcacga tgaacaacct
     9481 gacctttggc gacgcgacgc tgcagtacta cgagacgatc gcaggcgggg ccggcgcggg
     9541 cccggacttc ccgggcgcga gcgccacgca gacccacatg acgaactccc gcctcaccga
     9601 tccggaaatc ctcgagctgc gctacccggt gcgcgtgcgc gagttttcgc tgcgcacggg
     9661 atcgggcggt gccggccgcc agcgcggcgg tgatggcgtg gtgcgcgcca tcgagttcct
     9721 cgcgccgatg agcggcgcga tgctcgccaa tcgacgccgc acgcggccct tcgggctgcg
     9781 gggcggcgag ccgggcgccg ccggcatcac gcgcctgcgg cgcgcggcgg gcgggatcat
     9841 cgaactcgcg gccgccgacc gcttcgacgc gcaaccgggc gatgtgatcg aggtgctgac
     9901 gccgggtggc ggcggcttcg gcccgccgtc gcggacctag gcttcgagcg cgatcccccg
     9961 caggatcgcc gtttcgatct cctcctcgac ctgctcgccc gaggtggcga gtcggttcac
    10021 gctgaccggc cgcacgctgc tcgagcccgc ctggtgcgtc acgaggaagg catggaaatc
    10081 gccggaacgc atcggcgcga agccgagcag cagatgcacc ttgcccgccg cgaccgattc
    10141 gggcggattc aggcgctcgt cgcagatcac gaaccagcgc tcggcggccg tgcgcccgac
    10201 gaatgcgcct tcttcctcga tctcggcgag aaacgcggcg acttggttgc gcagtcgcgc
    10261 ccaggcggcc ggctggttgt gctggaagac gatccagcgc gtgccctcct cgaccgaggc
    10321 gaggatgaac agcgcaagtc gccgcgccgc gagataggtc cagtccgccg gccccgagcc
    10381 gccggcgccg agcgtgcgcg ggctgccggt tcgatgcagc gggcgcaccg tggcgaaggt
    10441 gttgacgccg agctgtgcaa gccggccacg ttcgacatcg tcgatctgcg tcgcggggcg
    10501 cagcgccggg cgcagcacgg gctcgtcgtt ttcgcccgcg ctccacaccg gcgtcgccgc
    10561 gtcgccgcgc gcgatgagcc cggccgcggc cccgcaggag gcgaacacct cgtggcgccc
    10621 gcgcacccga tcgaaggcga caacacgcgg gaagtacatc agcgcctgct cgctgcggaa
    10681 cggccagggg cgcagcgact cgaccgcacc ggcggcgctg gtccacgacg ccggcggatc
    10741 gacgatcagc atcgcatggc gctcgcggca caagcgcgag gccaccatca gcgtcgcgag
    10801 gcccacgtcg tcggtgcgcg tgagcggcgg cacgcacagc aggtcgaagc gcggcgcgaa
    10861 gcgcaggctg aagaggcccg tgccgcgctg gctcgagccg atgatgtcgt agtccgtgag
    10921 cgccgcgccg tcgtcgccgt cgggattcga tgcgacataa cctgccatca ggctcgaggt
    10981 gcgcgacggc gtgcgcagcg ggcgctgcgc cggcggcacc tcgccgagca cgcgcacgag
    11041 ctgcgaatcg agcagcacgg tggcgacgta gcggtcgctg cccggcagca ccgacaggcg
    11101 ccggtagatc tcctggtcct cgatgcgctc ggaacccggt gtgcgcaccc gttgcaccac
    11161 gaggttgaag cggtcctctt cgttctcgcc gatgccgtcg agatcgaccg acgcgcgcag
    11221 atactcgcgc gagcccggcg cgatgccgac cagggtgagc ggcgcaccgc ccgtggggag
    11281 cgtgatggtg ggcgggcggg cgctgttcgc gacgcgcacg acgatcgcgg tgcgcccgcc
    11341 gttctcgaaa tactgctcga gcgcgtaact catcgtcgag ggctgccaca gcccgccgaa
    11401 gaggcgcacg tactccgcga aattgtggat gacgacggga ttgttgaccg gacccttgag
    11461 tgcccggccg acgaaagcgg tgatgccagt ggccacgcgg gcgatcgcgt gccctggtcc
    11521 actttgttcc gagatgtcga agccagtgac ggcgtcggcc actgctgccc cccgtgaatc
    11581 cgtcggactg tagcataacg gcgacagtca cagtcggtca cgagctcgcg cttcacccct
    11641 cgcgcgcggg tgacgtgcgc acggatggcg ctacctgcgc gattcggtgg gggagtcctg
    11701 tcccgcggcg cgccgccgct gctcggcatt cgcggcgtcg agatcccgcc gcttgcgctc
    11761 gatctcgtcg agcatcagct tcagccattc gctgagacgt tgcccgcccg gcgcctgcgg
    11821 ctgggtgtcg ctcatggcgc gcccacgatc gcatcgagcg acgcggcggt gatcggatgg
    11881 tagccgggcc gcgcccgcgc atagaccgcg cgcgcgcgct gcgcgccgga ctgcgtcttc
    11941 atcatcgcgg tgtacagcgg cacgatcagc ttgcggcgcc cgatcgactc gaggtactgc
    12001 tcgagccgcg gccaggccgg ctcatagtcg ttgcgtatcg cgatctgcag ccagttgaag
    12061 gcgatctcgt tgttggtcgc ctgcgtgaag tcgaatgccg catcgagcga ctcgagctgc
    12121 gcgcgcggca tctgctgcgg aagattggcg aggaagtaca gccactcctg cgtactccag
    12181 ccggcgtgcg gcagatcggt tgccgcgcgc gtgccggcga ggaacgcggc gcgcgccgcg
    12241 tcgacctgca cgagcgcgtc ggatgcgggc cggatcgcgc tcgccggcag gcccggctga
    12301 tggatccact cgtcgagttc cgccgcggtc acggaatcgg ggtgctgcgc gagcaacttc
    12361 gagtcgaggt agccgaggaa ctgctcgacg gtgatgctct ggaacgcgaa atgcgcgaaa
    12421 tagtcctgca ggaatgcgtg cagcggggcg gcgccgaccc gggactcgag ccagcggagg
    12481 aagaagcgac ccttctcgta cggaacgtcg gtcacgccgt cgtcggggtc gcggccgcgc
    12541 aggtcgatgg cgaggacctg ctccgccggc ggcagggtcg cgagctcgcg ctcgagcgac
    12601 tgaaaaccga gcaggtcttc catggccgcc cggcgcggac catataccgc cgccatgatg
    12661 cggctttcga ggtagacggt gaatccctcg ttcagccaga aatcgcgcca ggtcgcgttc
    12721 gtcaccagat tgcccgacca ggagtgcgcg agttcatgcg cgatcagcgc gacgaggctg
    12781 cggtcgcccg cgatgacggt cggcgtgatg aaggagaggc gcgggttctc catgccaccg
    12841 aacgggaagg acggcggcag gatcagcacg tcgtagcgtc cccagcggta gggcccgaag
    12901 agcgactcgc cggtttcgag catcttttcg gtgtccgcga actcgcgggc ggccgcggcg
    12961 atgatctccg gctcggcgta gacccccgtg cgcgacccga tcggctggaa ttcgagtcgc
    13021 ccggccgcga tcgccatcag gtaggacgga atcggctgcg gcatgtcgaa acggaacacg
    13081 ccgtcggtgc ccgggccccc gaccggctct gcgcccatca gtgcgcgcag gcccgccggc
    13141 acgcgcagcg tggcctcgaa ggtgaagcgc acctgcggcg aatcctgcac cggtacccag
    13201 ctgcgcgcat ggatcgcctg cgactgcgaa tagaggaagg gtgccacctt gcccgcggtc
    13261 tgcgccggct cgacccattg caggcccgag gcatcgggtc gcgtcgcgta gcgaatgcgg
    13321 atcgtgaacc gtgcgaggtc accgggcagc gtgatcgcca gcggcgcgcc gagatgctcc
    13381 acctcggcac ccacgccgaa cgcgagcggc tgcggcgccg caccgggcgc gagccattcc
    13441 accgccgcga cgtcgaggcc gcgggtgtcg aggacgagcg tgcggccggt gcctgcgatg
    13501 cgctcgacgg tcagctccgc gccgccggcc agccgcttcg aatcgaagtc gacggtgaga
    13561 tcgagggcga gatgacggac gcggaactcg tccacgttcg cgagcgtgtg gtaatcgcgg
    13621 ccgctctgca gcggcgcggc cgcgctgtcg gcgacgggcc gggcggcgca ggacgcgagc
    13681 agcgcgaggc tggctgccgc gacgacgcgg atcatcgggc gcatggcgag gccgcgcatc
    13741 agcccttcgg cttccgcccg ggcgcattgg cgccggtggc gggtgccggc gccacgtcct
    13801 ggttgtcgga gaagatttcc tggaaggtcg cctcgtcgat cggcaacccg gacacgccgt
    13861 cccaggtggc accgtccggc acccggttct cgaccacctg tgccacttcg atctcggact
    13921 cgatgctgag cacaccgccg gcgatcggcg ccgggatacc gcgcggcgca tgtgcgatgg
    13981 ccgccaccgc gtcccagtcg acgctctcgc cgcgttcctt cagctgcttc tggatacgcg
    14041 tcgcgagcga cttcgacagc agcttcttcg gcgccttcgc gaagtcgcgc gtgtcctcct
    14101 cgagcacgtc ataggcctgc aggaacagct tgtcctcggc ccaaccgaac acgaatggct
    14161 ggttcacgac gcgcaccggc gtgccgaccg ccactttctc gaacagcggc tcgatgtctt
    14221 ccgggtacag gcggatgcag ccgtggctcg agcggatgcc gacgccgtag ggcttgttgg
    14281 tcccgtgcac gaggtaggtc ggccagccga gatacatcgc gcgattgccc agcggattat
    14341 cgggtccggc cggcacgacc gccggcaacg gatcgccgtt ttccgcgtgt tccttcagga
    14401 tcgagggagt cggccgccac acgggatcct tctgcttgcg cacgatcctg gtcgtgccct
    14461 cgggcgtcgc ccagcccacg cgcccgacgc cgatcggatg cgtgtgcacg acctgcggcg
    14521 catcgccctt gcgcggctcg tagtagtaga tgcgcatcgc ggccaggttg atgacgatgc
    14581 cctggcgcgg cgcgttcggc aggatgaagc gggtaggcac gaccacttcg cgtcccgcgc
    14641 ccggcagcca cgggtccacg cccggattcg cgcgcaccat ttcctcgtag ccgatgttga
    14701 agcgccgcgc gatgtcggag aaggtgtcgt cggcgccgac cacggtcttc tgcagcacgc
    14761 cgaccacgtc gtcgttcggg ccgatctcga aacggctggt cgcgaccggc ggcgcgtact
    14821 gcggctcggg cggcgcctcg ggctcggccg gtgccttgcg accgaacccg ctgccctcga
    14881 gcagcgaaca gccggcaagc gctgcgaggg tgagcacgca cgcgctggcg cgcaggaagg
    14941 ctgtagtccg gtgttgtctc gacatcagga aattatggca ttccctgcca catgctcaaa
    15001 gcaatacggg tgcattgcgc aattcgtcgt gatcgccgcc ctcgtgcgga aagtggcggc
    15061 tcaggacttc ccctacggcg cgcacgccat cggcgagccc ctcgccatgg cggccggcgc
    15121 gaaaggctgt ctcgatgtgg cgacagatgg ccgcccattc ggcacgcggc acgcgcgagg
    15181 cgatgccgcg gtccgcgacg atctcgacgt cgtgatcggc cataagcacg tagagcagca
    15241 cgccgttgtt gttggcggta tcccagacgc cgagcatgcc gaagaccgcg agcgcgcgct
    15301 gacgcgcggt gacgccgaac cagaggtcgg ccaggtcgag cgaggtctcg acggccacgc
    15361 ggatttcgcc ggcgtgccgg gactcgactt cgcccaccgc acgctcgatc gtcgcgcagg
    15421 ccaggcgatc gaagcgccgg cgtgtcatcc agtgcgaagc gaacaggtgc ctcagccagc
    15481 gcgcgagcga catcaccatc tccccgaggc gccgccgcca ccgaagccgc cgccaccccc
    15541 gcggaaaccg ccgccaccga agccgccccc gcccgagctg ccgccccagc cgccgcccca
    15601 accgccgtgg cgcgggccgc tcgaccagtt gccgccaccg agcgaggcga gcagcagcga
    15661 gagcagccat gccccgatgc cgaccgcgat cgcgatgccg agggcgctcg cgacgaggta
    15721 cacgagaccg ccggtgacgc cgccggtcag cagcgagccg attccgcgac cgaagatcga
    15781 ccgcaggatg ccggacgcga acagcacgcc gaacagcagg aacggcagca ggtcgagccc
    15841 gcgcgatacc gcatcagtgc cctgccagcg ctcatcgggt ggcggcagtt cctcgccgcg
    15901 caccacggcg aggatctggt cgacacccgc gtcgatgccg ccgtagtaat cgttctggcg
    15961 gaaatgcggc gtgatggttt cgcgaatgat gcggctcgcg atcgcatcgg gcagcgcgcc
    16021 ctcgaggccg tagcccacct cgatgcgcag ccggcgatcg tccttcgcga cgagcagcag
    16081 cacgccgtcg tccggttgcg cgcgcccgag cttccaggcg tcggcgacgc ggatcgagta
    16141 ctgcgcgatg tcctcgggtc cggtcgtcgg cacgagcagc accgcgacct gcgcgccctt
    16201 ctcccgctcg agcgcctcga gcttcgcttc gagcgccgcg cgctgcgcgg agtcgagcgt
    16261 gccggtggtg tcggtgacgc gcgcggtcag cggcgggacg ggcagcagat cctgcgccgg
    16321 agccggcgcg ccggcggcca gcgccgccag cgccagcgtg acgcccgcga gcagcgggcg
    16381 cgac
//
LOCUS       DAOQVY010000049        16023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_4139_length_16023_cov_5.200276, whole genome shotgun sequence.
ACCESSION   DAOQVY010000049 DAOQVY010000000
VERSION     DAOQVY010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16023
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4139_length_16023_cov_5.200276"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PKN91_06345"
     CDS             complement(<1..597)
                     /locus_tag="PKN91_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNS27726.1"
                     /translation="MTTRRILITGAGSGIGAELAQLLAAAGHEVIATDSDGAAALRVA
                     DAIDALGGKATGIALDVTQPAAIDTVVRAAGRVEVLVNNAGLQHVARLEDFPADRWAL
                     LVAVMLTGAANMARAVLPGMRERKFGRIVNIGSIHSLVASPYKSAYVAAKHGLIGLSK
                     TLALENADLDITINTICPVYVRTPLVERQIEAQAREHGL"
     gene            685..2598
                     /locus_tag="PKN91_06350"
     CDS             685..2598
                     /locus_tag="PKN91_06350"
                     /inference="COORDINATES: protein motif:HMM:NF022073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HNS27727.1"
                     /translation="MTTTHIAATAAAAALVVLAGCTAGSRLEPAPRATGSAAAAAVHR
                     AQRVGEPIRYEFDGVTDDLLTAGLGAAGLLGPAPGFADARNPGALELRRRAIYMNYRG
                     LADLAAAPGGGSAVAGVELLVGLYTPETGASTAMLQIPRDFDTANPCLVAVASSGSRG
                     IYGALATAGGWGLRHGCAVVHTDKGTGSGVFEVAGGRGVRLDGTLGTAGDPLLSFAPD
                     PAAVARLAAQRPQAVLFRHANSGVNVEAHWGEYLLQAIDVAFALLNREYPDRAAAFEP
                     ANTLVIAAGISNGGGTVLHALERDVAGWIDGAVVSEPNVSVPDTGAFGYPVRPLYELV
                     LEHLLLQPCAVLADEDATAPFRAATVAARPALEQWCRDLVARGELPAGEPDAIARAAR
                     DRLIALGIEPDALRLGHVNVHASLWSAVAVAYTSAYARLPPDELPCGVSFAATDAAGQ
                     PRALADEELARFFADSTGIAPTAGVNLVAADASGTLRVANFGSIDLALCLRGLHDRVA
                     PGLAAARATPRADPRPVILMHGRLDGLVPVGHSSRPWFHASIVAGGETAGVRYYEIER
                     GQHFDAFLPAPGFKDHYVAMQPYFDTAMDLMAAHLRTGSALPPSQVVRKGLVAAPGAD
                     AITAERDALLIPR"
     gene            2712..4346
                     /locus_tag="PKN91_06355"
     CDS             2712..4346
                     /locus_tag="PKN91_06355"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNS27728.1"
                     /translation="MTTPADHRELEWQLAATDLDGVRKWLMTKRPGAEGWYCKPRATV
                     EHHDIYLDTDDWRIHRAGFALRVRDASGRHEATLKELRPARDGFADRRELSEPLADAG
                     DEGVAAWVGPVGARVRAVAGARALRTLFHVRTQRERFGIGRAGSDAGAGELALDSTTI
                     SAPDGAVRATLQRVEVEASGNDSEPLVEFVLALRRECALEAAADNKYACGLAALALVP
                     PAAPELGPTGIDRDLRIDEVGYAVLRLGLLAWYANEPGARLGDDPEALHDLRVAGRRM
                     ESALGFFEADLPARLVRLRPRLKQVVRTLGATRDLDIALSQLEAFRRELEPFRQEALN
                     VLRGRLEAERLRARAKMLRALDAVATQRMLARLERLITLPAGQRRRSRHLPAVEVMPD
                     LLRARYRKLRKAIAPLAADSPAGELHAARGKVKNLRYATEAVTAIYGKPAEEFLRVLR
                     QLQNRLGEQHDAHVAQERLHALLRHPPRGVNGAMMFQFGRVAERYAAADRQARRGIAK
                     PRDRLRKRWKKLRRRLNEANAPVAIPARSLDPDSRP"
     gene            complement(4349..6637)
                     /locus_tag="PKN91_06360"
     CDS             complement(4349..6637)
                     /locus_tag="PKN91_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS27729.1"
                     /translation="MTIRETTPFAIAAIAGLALGGGDATALAQDASATATVALEEVIV
                     TTRRREESLQDVPAAVSAFSDAQLRELAINNLEDINSLAPNIKVNPGRATSNTINAYI
                     RGVGQNDPLWGFEPGVGIYIDDVYVARPQGALFDIYDVQRIEILRGPQGTLYGKNTLA
                     GAIKYVTRDIVGEPAFNATLAAGSYGQLDAKLAASTALGEHVYVGASLARLTRDGFGE
                     VVADANPYQFNSVGDDVSDKDILAARAEVNFLWGDSSRLRIMGDYIKDESNVRGGQRL
                     NDVFGPRLDDRYDVRNDMPVDEEDFENKGVSATFTTDINDQWALKVVGAYREGDGTTF
                     LDFETLNVNSFNVPAQYADDQTSFEVQATYTGERFKGVGGLYYFDGTAAGSFDAVLGG
                     LFGGLIQLTQGEVDTESYSAYFDGTFALTDRWNLNFGARYNEDKKDATVYNAQYLGQI
                     PSNTGMFDRNNLPPGIVLFSVLSDYTESRSDSKISPKLGVDFQATDDVMLYAQYSGGF
                     KSGGFDMRGNAAVFPGTRDGYKPETVDNYEIGIKGLLFDRRLMLNATVFYAEYEDVQV
                     TTQQFVEVMGVPSNATAVLNAGKQVNQGVELEATWRPVEAFTLSTAIGYLDADVKEFF
                     ASNPAAPGPPQNFESVTPVINAPEWTFSADMQYRFSVFGGTAIARAGYQWRDDTSIAP
                     PPFTLVSVTDQPSYDLLDLGLSYTTPDEHWRFAVDAKNVTDEWYRTAGYDFGTTGVFA
                     NMSQIGYYGPPRTYTFSVAYRY"
     gene            6750..8057
                     /locus_tag="PKN91_06365"
     CDS             6750..8057
                     /locus_tag="PKN91_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNS27730.1"
                     /translation="MLSLFGLLLGLSLLVWLTMRGTELLVAAPLCALLVAAMSGFAMF
                     PQLAAPDAPSFTASYMKGFVDFVQNWFFMFLLGSLFGKMMECTGAADSVANWIVAKLG
                     AKHAVGAVVAACAVLTYGGVSLFVVAFSVYPMAVSLFRRADLPRRFIPATIAFGSVTF
                     TMTSAGSPEIQNWIPIRYLGTTPYAGWEVSLVVALFMLALGQWWLLRSVRAASAGGER
                     FEARVADPEVHDRDLPAVWRGLVPLAVVLVAAFLLHEPLAESALIVALGAGVLVALTL
                     NYRYAHRVGAAVTAGALGALFAIANTSAVVGFGGVAKLTVGFQAAVEAMTSLPGSPLI
                     GAAIAVSVIAGLTGSASGGQSIALPLIAPHYLDQGVDPEALHRAVAISSGGLDSLPHN
                     GYVVTTIRAICGESYAAAYPSFAALTVVVPVLGTVLAVVLFSL"
     gene            8073..11579
                     /locus_tag="PKN91_06370"
     CDS             8073..11579
                     /locus_tag="PKN91_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HNS27731.1"
                     /translation="MLTPWFIALLGLAYVGVLFVVAHLGDRLSAEGRGQSARPWVYSL
                     SLAVYCTSWAFYGTGGQSAQLGWWLPPTYVGTILLFVFAAPFLDKMIRIGRRQKITSI
                     ADFIAARYGRDPRVAVLVTIVAVCGLTPYIALQLKAIDISFTALAGLDAPGAGGGAVF
                     GNTALYVTLSMAAFAILFGTRHADATEHHPGMMLALAFESIVKLVAFLAVGAFITFGL
                     FDGPVDIARRIAADPEVQAMREAARPAGHYLALTVLGVLAIFCLPRQFHVAVVENSGP
                     RDLRVARWLFPLYLIAISLFVLPVANAGLLAYGAGATNADLFLLHLPLANDQPALAML
                     TFVGGLSAATGMVIVACIALSTMICNEIVMPTLLHAMPSLRRRQDLTGLLLRVRRIAI
                     VLLLAAAYVYYRGIAYAGALAEIGEIAMAAVALFGPLVFAALYWKRASSAGALAGLAC
                     GFAAWLYTLIVPVFADVGYLPAGIVTAGPFGLEWLRPTALLGFEGLNPLAHGLAASLL
                     VTTLALVIGSLTLRRTLVDRIQAAVFVDATDDGGGEHYHTHAGALTLKDLRALVGQFV
                     GQHRIDAHFAQFRSSAGESASAGQLESVQRLLGSVIGAASARRVIDAAASGKDLVFED
                     VVSIVDSASRALEFNRELLQATLENVSQGISVVDADLRLVAWNQRYATLFDYPPDMLQ
                     IGKPIEEVLQYNAARGALSGDDVDKLVARRMAHLRRGTDYVYQRNSPDGTVLEIRGNR
                     MPGGGFVTSYTDVSEYKRAEAALKEINESLERRVTERTRELAAAKVEAERANLSKTRF
                     LAAASHDVLQPINAAGLFAGALLQKLERPAQKALVGDIEQCLRTAEGVLTDLLDISKL
                     DAGVVQAQVGELPVQELLGALEGEFGMTARARGIELRVRGSSAVVLSDARLLRRILQN
                     FVANAIRYTAQGRVLVGCRRRGEQLRIEVWDSGSGIAADQLDRIFEEFYRAPTAGAAD
                     AVSGKGFGLGLAIADRIARRLGHRLGVRSTVGRGSVFTVEVPLGSARGAIVRPLRRTR
                     AVGSLNGARALVIDNDQAVLRAMQVLLESWQCEVRAAASAAELPDFAGWQPDVMIVDY
                     HLDHGVTGVALAAEIRARAGFQLPLIVITADAADAIREEVEASGGLCMRKPVRPLALH
                     NVLRRVVLHGPDQRSRESWSAS"
     gene            complement(11546..12223)
                     /locus_tag="PKN91_06375"
     CDS             complement(11546..12223)
                     /locus_tag="PKN91_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNS27732.1"
                     /translation="MDESASRTVLIADDHPLFRAALRRAVEEAVGDARVIEAADVDAL
                     RADVSAHPDADLVLLDLMMPGSRSLAPLAWLRSQHPGVAVIVVSGTENAAVMRAALEF
                     GAAGFVPKSAPLADLVAAIRAVLDFTQWFPAAAYEDPAGPEGGTASLAARFASLTPQQ
                     FRVLELLARGKLNKQIAAELGITEQTTKAHVSAALAKLGARNRTQATLLYGSLEIQDA
                     DQLSLDR"
     gene            12330..13619
                     /locus_tag="PKN91_06380"
     CDS             12330..13619
                     /locus_tag="PKN91_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNS27733.1"
                     /translation="MVSAELLVIFVLVLLNGFFALSEMALVSSRRARLQSRAEQGSRG
                     ARIAVALLEDSTSFLSAVQIGITLIGIVTGVYSGATLAAHLDDVIAGWGVPMTYAEEI
                     AFVVIVVVITFVTLIFGELVPKRIALNNSESLAIAVAPVMLGFARLMTPFVWLLRGAV
                     ELVLKVLPVSSASEQSVTEDDVRAMIAEGTRSGVFLASEKRLIEGVLALADRHVTTVM
                     VPRQDVVWLDLDDPLPELWQTAKHSGHARFLVAHGSLDQLAGMVTLANLSEALRRGAL
                     DAEHDIEPALHVPDTLTVMQLLDQFQRSSTHLAVITDEYGEIEGVATPIDILKAIAGE
                     LPDVGSRERPEALPREDGGWLVDGHLPIDELQRALGRRDMASGDYHTAAGFVLARLGR
                     IPKVGELLTWRGLRFEVVDMDGRRIDKLAVAIVKAPE"
     gene            complement(13603..14745)
                     /locus_tag="PKN91_06385"
     CDS             complement(13603..14745)
                     /locus_tag="PKN91_06385"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNS27734.1"
                     /translation="MDCEPKPRRSPSHSGTTSLHARSLGALALALLFAGCATVPPPSP
                     PAPEAAPPPPAPPPTVISIAAVGDIMLGTDFPKNILPDDDGIGFLRHVTPILAGADVA
                     FGNLEGVLMDGGEPVKQCGSSTSCFVFRTPTRYAAYLRDAGFDLVSLANNHARDFGEE
                     GRDSSMAALDAVGIRHSGREGTWAGMTVKGVRVAMVAFAPNVGANSLNDHELAVEFVR
                     SLAAANDIVIVSFHAGGEGAGAEALPYAREFYAGEDRGDVVMFARAMIDAGADLLLGH
                     GPHVPRAMELYRDRLIAYSLGNFATYYGIGVEGNRGIAPIVTARLDAEGRFVGGHIDS
                     TIQLRPDGPSIDPQQRALQLIRELTAAALPYGWLEFGADGRIIRVP"
     gene            complement(14745..15320)
                     /locus_tag="PKN91_06390"
     CDS             complement(14745..15320)
                     /locus_tag="PKN91_06390"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNS27735.1"
                     /translation="MRLTARDFKRSRSGSVEFARWRQFGLGLATGLGVALLVYVVMNQ
                     RALQTARDVALERPEPRVAAAPADEQVAEVPGEEAGRQYDFYEMLPKFEVVVPERDRE
                     VKPDAPAAVERPGVYVLQAGSYRGQADAERVRSQLALQGIQAAVQRVAVDADVWHRVR
                     IGPIADLAELNRVRARLRANDIDALVIRVGD"
     gene            complement(15342..>16023)
                     /locus_tag="PKN91_06395"
     CDS             complement(15342..>16023)
                     /locus_tag="PKN91_06395"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HNS27736.1"
                     /translation="DLLVDVLGADHRGYVARVRGGLVAMGQPADCLEVILIQFVSLWR
                     GEVKIPMGKRDAQFVTLRQLREEVGTDACRFFYLMRSHDQPLDFDLELAKSRSNENPV
                     YYIQYAHARVSSVMKQLVAKGYRYDEAVARANLGLLTGEHEQAALTALVRYPEMLCQA
                     AANRTPHTLVHYLRDLANTFHTWYNAAQFIVEDPPLRNARLALALGVQQVIRNGLGVL
                     GVSAPETM"
BASE COUNT         2289 a         5431 c         5632 g         2671 t
ORIGIN      
        1 gagcccgtgt tcgcgagcct gcgcctcgat ctgcctctcg acgaggggcg tgcgcacgta
       61 gaccgggcag atggtgttga tcgtgatgtc gaggtcggcg ttctcgagcg caagcgtctt
      121 cgagaggccg atcaggccat gcttcgccgc cacgtaggcg ctcttgtagg gcgaggcgac
      181 cagcgaatgg atcgagccga tattgacgat gcgcccgaat ttgcgctcgc gcatgccggg
      241 cagtaccgcg cgcgccatgt tggccgcgcc ggtgagcatc accgcgacca gcagggccca
      301 gcgatcggcc gggaagtcct caagccgcgc gacatgctgc aggccggcgt tgtttaccag
      361 cacctcgacg cgccccgcgg cgcgcaccac cgtgtcgatg gcggccggct gcgtcacgtc
      421 gagggcgata ccggtggcct tgccgccgag ggcatcgatt gcgtcggcga cacgcagcgc
      481 ggcggcgccg tcgctgtcgg tggcgatgac ttcgtggccg gccgcggcca gcaactgcgc
      541 gagttcggca ccgatgccgc tgccggcgcc ggtgatgagg atgcgtcgtg tcgtcatggg
      601 ctcaagctac cggagcgggc cgcgtgccgg cgctgctacc ttagtactat tctgcatcga
      661 cgcgcgaggc gttgaacttc ccgcatgaca accacacaca tcgccgccac cgcggctgcc
      721 gctgccctcg tcgtgctggc cggatgcacg gcgggatcac gcctcgagcc tgcaccgcgc
      781 gcgaccggga gcgccgccgc cgccgccgtg caccgcgcgc agcgagtcgg tgagccgatc
      841 cgctacgagt tcgatggtgt caccgacgac ctgctgaccg cgggcctcgg cgccgccggc
      901 ctgctggggc cggcgccggg attcgccgat gcgcgcaatc cgggcgcgct cgagctgcgc
      961 cggcgggcga tctacatgaa ttaccgcggc ctggccgacc tcgccgcagc ccccgggggt
     1021 gggtcggcgg tggcgggcgt cgagctgctc gtcgggctgt acacgccgga gaccggcgcg
     1081 tcgacggcga tgctgcagat cccgcgtgat ttcgacaccg ccaatccctg cctggtcgcg
     1141 gtcgcgagtt ccggctcgcg cggcatctat ggtgcgctgg caaccgccgg cggctggggc
     1201 ctgcgtcatg gctgcgccgt ggtgcatacc gacaagggaa cgggcagcgg cgtgttcgag
     1261 gtggcaggcg gccgcggggt gcgcctcgac ggcacgctcg gtaccgcagg cgatccgctg
     1321 ctgtcgttcg cgcccgatcc ggcggccgtg gcccggcttg cggcgcagcg gccgcaggcc
     1381 gtgctgttcc gccacgccaa ctccggcgtc aatgtcgaag cgcactgggg cgagtacctg
     1441 ctgcaggcga tcgacgtggc attcgcactg ctgaaccgcg agtacccgga tcgcgccgcc
     1501 gctttcgagc cggccaacac cctggtgatt gccgccggca tctcgaacgg cggcggcacc
     1561 gtgctgcacg cgctcgagcg cgacgtggcc ggctggatcg acggcgccgt cgtctcggaa
     1621 ccgaatgtct cggtgcccga caccggggct ttcggctatc cggtgcggcc gttgtatgag
     1681 ctggtgctcg agcatctgtt gctgcagccc tgcgcggtgc tggcagacga ggatgcaacg
     1741 gcgcccttcc gcgccgcgac cgtcgcggcg cggccggcgc tcgagcagtg gtgccgcgat
     1801 ctcgtggcgc gcggcgagct gccggcgggt gagcccgacg ccatcgcccg cgccgcgcgc
     1861 gatcgcctga tcgcgctcgg catcgagccc gatgcgctgc gactcgggca cgtgaacgtg
     1921 cacgcctcgc tgtggagcgc ggtcgcggtg gcctatacct cggcgtacgc gcgcctgcca
     1981 cccgatgaac tgccctgcgg cgtgagcttc gcggcgaccg atgccgccgg ccagccgcga
     2041 gcgctcgccg acgaggaact cgcgcgcttc ttcgccgata gcaccggcat cgcaccgacc
     2101 gccggcgtca atctcgtcgc ggccgacgcg agtggcacgc tgcgcgtcgc gaatttcggc
     2161 agcatcgatc tcgcgctctg cctgcgcggc ctgcacgatc gcgtggcacc gggcctcgct
     2221 gcggcgcgcg ccacgccgcg tgccgacccg cgccccgtga tcctgatgca cgggcgcctg
     2281 gacggactcg tgccggtggg ccacagctcg cgaccctggt tccacgcgag catagtcgcc
     2341 ggtggtgaga cggccggcgt gcgctactat gaaatcgagc gcggccagca cttcgacgcg
     2401 ttcctgccgg cgcccggttt caaggaccac tatgtcgcga tgcagccgta cttcgatact
     2461 gcgatggacc tgatggccgc gcacctgcgc acgggcagcg cgctgccgcc gagccaggtg
     2521 gtgcgcaagg gcctggtcgc cgcaccgggc gcggatgcga tcacggctga acgcgacgct
     2581 ctgctcatcc cccgctgagt gataccgatg agcgatttct cgacttgcga cctgtgcgac
     2641 gcccatgcgg aggagctgca ggtcgcggtg ctcggcctcg gtgtacgcgc gctggtgaag
     2701 gccgtgccga tgtgaccaca cccgccgatc accgcgagct ggaatggcag ctggctgcca
     2761 cggatctcga cggtgtgcgg aaatggctga tgacgaagcg ccccggcgcc gaaggctggt
     2821 actgcaagcc gcgcgccacg gtcgaacatc acgatatcta tctcgacacc gacgactggc
     2881 gcatccatcg cgcgggcttt gcgctgcgcg tgcgcgatgc ctcaggcagg catgaggcga
     2941 cactgaagga gctgcggccg gcgcgggacg gctttgccga ccggcgcgag ctgtccgaac
     3001 cgctggcgga tgccggggac gagggcgtgg ccgcttgggt gggccccgtc ggtgcgcgcg
     3061 tgcgcgcggt ggcgggcgcc cgggccctgc gcacactctt tcacgtgcgc acgcagcgcg
     3121 agcgcttcgg gatcgggcgc gctggcagtg acgcaggcgc cggcgaactg gcgctcgatt
     3181 ccacgacgat ctccgcgccg gacggcgcgg tgcgcgcgac gctgcaacgg gtcgaggtcg
     3241 aagcgtcggg caacgacagc gagccgctgg tcgaattcgt gctcgcgctg cggcgcgagt
     3301 gcgcgctgga agcggcagcc gacaacaagt atgcctgcgg cctggcagcg ctcgcactcg
     3361 tgccacccgc agcgcctgag cttgggccca cggggatcga tcgcgacctg cgcatcgacg
     3421 aagtgggcta cgccgtgctc cgcctgggtt tgctggcgtg gtatgccaac gagcccggcg
     3481 cgcggctcgg cgacgacccg gaggcgctgc acgacctgcg tgtcgcaggc cggcgcatgg
     3541 agagtgcgct cggtttcttc gaggccgatc tgccggcaag gctcgtgcgc ctgcggccgc
     3601 ggctcaagca ggtcgtgcgc acgctcggcg ccacccgtga cctggacatc gcactctcgc
     3661 agctcgaggc attccggcgc gagctggagc ctttcaggca ggaggcgctg aacgtgctgc
     3721 gggggcgcct cgaggccgag cgcctgcgtg cccgggcaaa gatgctccgc gcgctcgatg
     3781 cggtcgccac ccagcgcatg ctggcgcggc tcgaacggct gatcacgctg ccagccgggc
     3841 agcgcaggcg ttccaggcac ctgccggcgg tcgaggtcat gccagacctg ctgcgggcgc
     3901 gctaccgcaa gttgcgcaag gcgattgcgc cgctggcagc ggattccccg gccggcgaac
     3961 tccacgccgc gcgcggcaag gtgaagaacc tgcgctacgc caccgaggcg gtgacggcga
     4021 tctacggcaa acccgccgag gagttcctgc gtgtgctgcg gcaattgcag aaccgcctgg
     4081 gcgagcagca cgacgcccat gtggcgcagg agcgcctgca cgcgctcctg cggcacccac
     4141 cgaggggcgt caacggcgca atgatgttcc agtttggccg ggtggccgaa cgctatgcgg
     4201 cggcagaccg gcaggcgcgg cgcgggatcg ccaagccgcg ggacagactg cgcaaacgct
     4261 ggaagaagct gcgacgcagg ctgaatgagg cgaacgcgcc ggtagcgata ccggcgcgtt
     4321 ctctggatcc cgactccaga ccctgacctc agtaccggta cgccaccgag aaggtgtacg
     4381 tgcgcggcgg tccgtagtag ccgatctggc tcatgttcgc gaacacgccc gtggtgccga
     4441 agtcgtagcc tgcggtgcga taccactcgt cggtcacgtt cttcgcatcg accgcgaagc
     4501 gccagtgttc gtcaggcgtc gtgtacgaca ggccgaggtc gagcaggtcg tacgacggct
     4561 ggtcggtgac gctcacgagc gtgaacggtg gcggggcgat cgaggtgtcg tcgcgccact
     4621 ggtaacccgc ccgcgcgatc gccgtaccgc cgaatacgct gaagcggtac tgcatgtcgg
     4681 ccgagaaggt ccactccggc gcgttgatca ccggtgtgac cgactcgaag ttctgcggcg
     4741 gaccgggcgc ggccgggttc gacgcgaaga actccttgac gtcggcatcg aggtagccga
     4801 tggccgtgga cagagtgaag gcctcgaccg gccgccaggt cgcctcgagc tccacgccct
     4861 ggttgacctg tttgccggcg ttcagcaccg cggtcgcgtt gctcggcacg cccatcacct
     4921 cgacgaactg ctgggtcgta acctgcacgt cctcgtactc ggcatagaat accgtggcgt
     4981 tcagcatcag gcggcgatcg aacagcaggc ctttgatgcc aatctcgtag ttgtcgaccg
     5041 tctccggctt gtagccgtcg cgggtgccgg ggaacaccgc ggcattgccg cgcatgtcga
     5101 agccgccgct cttgaagccc ccgctgtact gcgcgtagag catcacgtcg tcggtcgcct
     5161 ggaagtcgac gcccagcttc ggcgagatct tgctgtcgct gcggctctcg gtgtaatcgc
     5221 tcaggaccga gaacagcacg atgccgggcg gcaggttgtt gcgatcgaac atgccggtgt
     5281 tcgacgggat ctggccgagg tactgcgcgt tgtagaccgt ggcgtccttc ttgtcttcgt
     5341 tatagcgcgc gccgaaattc aggttccagc gatcggtcag cgcgaaggta ccgtcgaaat
     5401 aggccgagta gctttcggtg tccacctcgc cctgggtcag ctggatcagc ccgccgaaca
     5461 ggccgccgag cacggcgtcg aaggagccgg cggcggtgcc gtcgaagtag tacaggccgc
     5521 cgacgccctt gaagcgctcg ccggtgtagg tcgcctgcac ctcgaagctc gtctggtcgt
     5581 ccgcatactg tgctggcacg ttgaacgagt tgacattgag ggtctcgaag tcgaggaagg
     5641 tcgtaccgtc gccctcgcga taggcgccga cgaccttgag tgcccactgg tcattgatgt
     5701 cggtcgtgaa ggtggccgag acgcccttgt tctcgaagtc ctcctcgtcg accggcatat
     5761 cgttgcgcac gtcatagcgg tcgtcgaggc gcgggccgaa cacgtcattc agccgctggc
     5821 cgccacggac gttcgattcg tccttgatgt aatcgcccat gatgcgcagg cggctcgagt
     5881 cgccccacag gaagttcacc tcggcgcgcg cggcgaggat gtccttgtcg ctgacgtcgt
     5941 cgccgacgct gttgaactgg taggggttcg catcggccac cacttcgccg aagccgtcgc
     6001 gggtgagccg cgcgagcgag gcgccgacat acacatgttc gccgagggcg gtcgaggccg
     6061 cgagcttcgc gtcgagctgc ccgtagctgc cggccgcgag cgtcgcgttg aaggcgggtt
     6121 cgccgacgat gtcgcgggtc acgtacttga ttgcgccggc cagcgtgttc ttgccgtaga
     6181 gcgtgccctg cgggccgcgc aggatctcga tgcgctgcac gtcgtagatg tcgaacaggg
     6241 cgccctgcgg gcgcgcgacg tacacgtcgt cgatgtagat gccgacgccg ggctcgaagc
     6301 cccacagcgg atcgttctgg ccgacgccgc ggatgtaggc attgatcgta ttgctggttg
     6361 cgcggccggg gttgaccttg atgttcggcg cgaggctgtt gatgtcctcg aggttgttga
     6421 tcgcgagttc acgcaactgc gcatcggaga atgccgagac cgcggccggc acgtcctgca
     6481 ggctctcctc gcgccggcgg gtcgtgacga tgacctcctc cagtgccacg gtggcggtgg
     6541 cggaggcgtc ctgggcgagc gctgtcgcgt cgccgccacc aagcgcgagg ccggcaattg
     6601 ccgcgatggc gaacggcgtc gtttctctga tcgtcatggg gtcccccctt gcatgaatag
     6661 ccgggaccgt acgccgcccc tcccgtcgct gcattggtac gttagtacta tggaggcccc
     6721 gcccccggac cgggcaccgt ggcggcccta tgctcagtct cttcggactg ctgctcgggc
     6781 tctcgctgct cgtgtggctc accatgcgcg gcaccgagct gctggtcgcg gcgccgctgt
     6841 gcgcgctgct ggtcgcggcc atgtcgggat tcgcgatgtt cccgcagctc gccgcacccg
     6901 atgcgccgag ttttaccgcg agctacatga agggcttcgt cgacttcgtg cagaactggt
     6961 tcttcatgtt cctgctcggt tcgctgttcg gcaagatgat ggagtgcacc ggcgcggccg
     7021 acagcgtcgc caactggatc gtcgcaaagc tcggtgcgaa gcacgcggtc ggcgccgtcg
     7081 tcgccgcttg cgccgtgctg acctacggcg gcgtgagcct gttcgtcgtc gcgttctccg
     7141 tctacccgat ggcggtgagc ctctttcgcc gcgccgacct gccgcgccgc ttcatccccg
     7201 cgacgatcgc cttcggttcg gtcaccttca cgatgacctc ggccggctcg cccgagatcc
     7261 agaactggat cccgatccgc tatctcggca cgacgccgta tgcgggctgg gaagtcagcc
     7321 tcgtcgtcgc gctgttcatg ctcgcgctcg gccagtggtg gctgctgcgc tccgtacgtg
     7381 ccgcgagcgc cggcggcgaa cgcttcgagg cgcgcgtggc agatcccgaa gtacatgacc
     7441 gggatctgcc ggccgtatgg cgcgggctcg tgccgctcgc ggtcgtgctg gtcgctgcct
     7501 tcctgttgca cgagccactc gcggaatcgg cgctgatcgt cgcgctcggc gccggcgtgc
     7561 tggtcgcgct cacgctgaac taccgttacg cgcaccgcgt cggcgcggcc gtgacggccg
     7621 gtgcgctcgg cgcgctgttc gcgatcgcca atacgtcggc cgtggtcggt ttcggcggcg
     7681 tcgcgaagct cacggtcggc ttccaggccg ccgtcgaggc gatgacgagc ctgccgggca
     7741 gtccgctgat cggcgctgcg atcgccgtca gcgtgatcgc gggtctcacg ggctccgcct
     7801 ccggcggcca gtcgatcgcc ctgccgctga ttgcaccaca ctatctcgac cagggcgtgg
     7861 accccgaggc cctgcaccgg gcggtcgcca tttcctcggg cggcctcgat tcgctgccgc
     7921 acaacggtta cgtcgtgacg acgatccgcg cgatctgcgg cgagtcgtac gctgcggcct
     7981 atccgtcctt tgcggccttg acggtcgtcg ttcccgtgct cgggacggtc cttgcggtgg
     8041 tgcttttctc gttgtgagac gataacggtg gcatgttgac accgtggttc atcgcgctcc
     8101 tcgggctcgc ctatgtcggc gtgctgttcg tcgtcgcgca cctcggtgat cgtctgtcgg
     8161 ccgaaggccg cgggcagtcc gcgcgcccgt gggtctacag cctctctctc gccgtgtact
     8221 gcacctcgtg ggctttctac ggcaccggcg ggcaatccgc acagctcggc tggtggctgc
     8281 cgccaaccta tgtcggcacg atcctgcttt tcgtgttcgc cgcgccgttc ctcgacaaga
     8341 tgatccgcat cggccgcagg cagaagatca cctccatcgc cgacttcatc gccgcacgct
     8401 atggccgcga ccctcgtgtc gcggtgctcg tcacgatcgt cgcggtgtgc ggtctgacgc
     8461 cctacatcgc gctgcagctg aaggcgatcg acatcagctt cacggcgctc gccgggctcg
     8521 atgcgccggg agcaggcggc ggtgcggtgt tcggcaatac cgcgctgtac gtgaccttgt
     8581 cgatggcggc gttcgcgatc ctgttcggta cgcgccatgc cgatgccacc gagcatcacc
     8641 cgggcatgat gcttgcgctc gcgttcgagt cgatcgtgaa gctggtggcg tttctcgcgg
     8701 tgggcgcgtt catcaccttc ggcctcttcg acggtcccgt cgatatcgca cggcgcatcg
     8761 ccgccgatcc agaggtgcag gcgatgcgcg aggcggcgag gccggcgggc cattacctcg
     8821 cgctgaccgt gctcggtgtg ctcgcgatct tctgtctgcc acggcagttc catgtcgcgg
     8881 tggtcgagaa ctccgggccg cgcgatctgc gggtcgcacg ctggctgttc ccgctgtacc
     8941 tgatcgcgat cagcctgttc gtgctgccgg tcgcgaatgc gggactgctc gcctacggtg
     9001 ccggggccac caacgcggac ctgttcctgc tgcacctgcc gctcgccaac gaccagcccg
     9061 cgctcgccat gctcaccttc gtcggcggcc tctcggcggc gaccggcatg gtcatcgtgg
     9121 cgtgcatcgc gctctcgacg atgatctgta acgaaatcgt gatgccgacg ctgctgcacg
     9181 cgatgccgtc gctgcgtcgc cggcaggacc tgaccggcct gctgctgcgc gtgcgccgca
     9241 tcgccatcgt gctgctgctc gctgccgcct atgtctacta tcgcggaatc gcctacgcgg
     9301 gtgcgctcgc cgagatcggc gagatcgcga tggcggccgt cgcgctgttc gggccgctgg
     9361 tgttcgccgc gctctactgg aaacgtgcga gcagcgcggg cgcgctcgcc gggctcgcct
     9421 gcggctttgc ggcgtggctc tacacgctga tcgtgccggt gttcgccgac gtcgggtacc
     9481 tgccggccgg catcgtgacg gccggcccat tcggcctcga atggctgcgc ccgaccgcac
     9541 tgctggggtt cgaaggactg aacccgttgg cgcacggcct cgccgcgagc ctgctcgtca
     9601 ccacgctcgc gctcgtgatc ggctcgctga cgctgcggcg cacgctggtc gatcgcatcc
     9661 aggccgcggt gttcgtcgac gccaccgacg acggtggcgg cgagcactac cacacgcatg
     9721 ccggtgcgct cacgctgaaa gacctgcgcg cgctcgtcgg ccagttcgtc ggccagcacc
     9781 gcatcgatgc gcacttcgca cagttccgtt cctccgcggg cgagagtgcg agtgccgggc
     9841 agctcgaatc ggtgcagcgg ctgctcggct cggtgatcgg ggccgcctcg gcgcggcgcg
     9901 tgatcgacgc ggcggcatcg ggcaaggacc tcgtgttcga ggacgtggtc tcgatcgtcg
     9961 acagcgcctc gcgcgcgctc gagttcaacc gcgagctgct gcaggcgacg ctcgagaatg
    10021 tgagccaggg catcagcgtg gtcgacgcag acctgcggct ggtcgcctgg aaccagcgct
    10081 acgcgaccct gttcgactat ccgccggaca tgctgcagat cggcaagccg atcgaggaag
    10141 tactgcagta caacgcggcg cgtggggcgc tgagcggcga cgatgtcgac aagctcgtcg
    10201 cacgacgcat ggcccacctg cgtcggggca ccgactacgt ctatcagcgc aattcgcccg
    10261 acggcacggt gctcgagatc cgcggcaacc gcatgccggg cggcggcttc gtcacgagct
    10321 acaccgatgt gtcggagtac aagcgcgccg aggcggcgct gaaggagatc aacgaatcgc
    10381 tcgagcggcg cgtcaccgag cgtacccggg aactcgccgc ggcgaaggtc gaggcggagc
    10441 gcgcgaacct gagcaagacg cgcttcctcg ccgcagcgag ccacgacgtg ctgcagccga
    10501 tcaacgccgc aggcctgttc gccggcgcac tgctgcagaa gctcgagcgg cccgcgcaga
    10561 aagcgctggt cggcgatatc gagcaatgcc tgcgtaccgc ggagggcgtg ctcaccgacc
    10621 tgctcgacat ttcaaagctc gatgccggcg tagtgcaggc gcaggtcggc gagctgccgg
    10681 tacaggagct gctcggcgcc ctggagggcg agttcggcat gacggcccgc gcgcgcggca
    10741 tcgaactgcg cgtgcgcggc tcgagcgcgg tcgtgctgag cgacgcgcgg ctgttgcgcc
    10801 gcatactgca gaacttcgtt gcgaacgcga tccgctacac ggcgcagggt cgcgtgctgg
    10861 tcggctgccg ccgtcgcggc gagcagctgc gcatagaggt ctgggacagc ggcagcggta
    10921 tcgcggccga ccagctcgat cgcatcttcg aggagttcta tcgcgcgccc acggcgggtg
    10981 cggccgatgc cgtctcgggc aagggcttcg gcctgggact cgccatcgcc gatcgcatcg
    11041 cgcgccgcct cgggcatcgc ctcggcgtgc gctcgaccgt ggggcgcggc tcggtgttca
    11101 cggtcgaagt gccgctcggc agtgcgcgcg gcgcgatcgt gcgcccactg cggcgcacgc
    11161 gcgcggtggg ctcgctcaat ggcgcacgcg cgctggtgat cgacaacgac caggcggtgc
    11221 tgcgcgcaat gcaggtgctg ctcgagtcct ggcagtgcga ggtacgcgcc gcggcatcgg
    11281 cggcggagtt gcccgacttc gccggctggc agcccgacgt gatgatcgtc gactatcacc
    11341 tcgatcatgg cgtgacgggt gtggcgctcg ctgcggaaat ccgcgcacgc gcggggtttc
    11401 agctgccact catcgtgatc accgccgatg cggcagacgc catcagggaa gaagtggagg
    11461 cgtccggtgg cctgtgcatg cgcaagcccg tgcggccgct cgccctgcac aatgtgctgc
    11521 ggcgcgtggt gctgcacggc cccgatcagc gatcgaggga gagctggtcc gcgtcctgaa
    11581 tctcgagcga cccgtataga agagtcgcct gggtgcgatt gcgcgcgccg agcttggcga
    11641 gcgccgccga cacatgcgcc ttggtcgtct gctcggtgat gccgagctcg gccgcgatct
    11701 gcttgttcag cttgccgcgc gcgagcaact cgagcacgcg aaactgctgc ggcgtgaggc
    11761 tcgcgaaccg cgccgcgagc gatgcggtgc cgccctccgg cccggccggg tcctcgtacg
    11821 cggccgccgg gaaccactgc gtgaaatcga gcactgcgcg gatcgcggcg accaggtcgg
    11881 cgagcggcgc cgacttcggc acgaagcccg cggcaccaaa ctcgagcgcc gcgcgcatta
    11941 ccgccgcgtt ctcggtgccg gagacgacga tcaccgcgac gccgggatgc tggctgcgca
    12001 gccaggcgag cggcgccagg ctgcggctgc ccggcatcat gagatcgagc agcacgaggt
    12061 cggcatccgg atgggccgac acatcggcgc gcagggcgtc gacgtccgcc gcttcgatca
    12121 cgcgcgcatc gcccaccgct tcctcgacgg cgcgccgcag ggcggcgcga aacagcgggt
    12181 gatcgtccgc gatcaggact gttcggctgg cggattcatc catgacctgt caccgtgggt
    12241 gatgagccca agtctaaggc acatgatgcg ggaaggagag gcgggccggc ctgtgctagg
    12301 ctcttgcgcc cggcatcgcc gctccgccca tggtctccgc cgaacttctc gtcatcttcg
    12361 tcctcgtcct gctgaacggc ttctttgcgc tgtcggagat ggcactcgtg tcctcgcgcc
    12421 gcgcgcggct gcagtccagg gccgaacagg gcagccgcgg ggcgcgtatt gcggtcgcgc
    12481 tgctcgagga ttccacctca ttcctgtccg cggtgcagat cggcatcacg ctgatcggca
    12541 tcgtcaccgg tgtctacagc ggtgcgaccc tcgcggcgca tctggacgac gtgatcgccg
    12601 gctggggcgt accgatgacc tacgccgagg agatcgcttt cgtggtcatc gtcgtcgtga
    12661 tcaccttcgt gacgctgatc ttcggcgagc tggtgccgaa gcgcatcgcg ctcaacaatt
    12721 ccgagtcgct cgccatcgcg gtcgcgccgg tgatgctcgg cttcgccagg ctgatgacgc
    12781 cgttcgtctg gctgctgcgc ggtgcggtcg agctcgtgct gaaggtgctg ccggtctcgt
    12841 cggcctcgga acaatcggtc actgaagacg acgtgcgggc gatgatcgcc gagggcacgc
    12901 gctcgggcgt gttcctcgcg agcgaaaagc ggctgatcga gggcgtgctc gcgctggccg
    12961 accggcatgt gacgacggtg atggtgccgc gccaggatgt cgtgtggctc gatctcgacg
    13021 acccgctgcc ggaactgtgg cagacggcga agcacagcgg gcacgcgcgt ttcctcgtcg
    13081 cccatggctc gctcgaccag ttggcgggca tggtgaccct tgcgaacctg agcgaggcac
    13141 tgcgtcgcgg tgcgctcgat gcggagcacg acatcgagcc cgcgctgcat gtgccggaca
    13201 cactcacggt gatgcagctg ctcgaccagt tccagcgctc gagcacgcac ctggccgtga
    13261 tcaccgacga gtacggtgaa atcgagggcg tcgcgacgcc gatcgatatc ctgaaggcca
    13321 tcgccggcga attgccggac gtcggcagcc gggaacgccc cgaagcgctg ccgcgcgagg
    13381 acggcggctg gctggtcgac ggtcatctgc cgatcgacga actgcagcgc gctctcggcc
    13441 ggcgcgacat ggcgtccggc gactatcaca ccgcggcggg tttcgtgctg gcgcgactcg
    13501 ggcgcattcc gaaggtgggc gagctgctga cctggcgcgg gttgcgtttc gaggtggtcg
    13561 acatggacgg gcgccgcatc gacaaactcg cggtggcgat cgtcaaggca cccgaatgat
    13621 ccgcccgtcg gcgccgaact cgagccagcc gtagggcaat gcggcggccg tcagctcgcg
    13681 gatgagctgc agcgcacgct gttgcgggtc gatcgatggt ccgtcggggc gcagctggat
    13741 cgtcgaatcg atatgcccgc cgacgaagcg gccttcggca tcgagccgcg ccgtgacgat
    13801 cggcgcgatg ccgcgattgc cctcgacgcc gatgccgtaa taggtcgcga aattgccgag
    13861 gctgtaggcg atcagccggt cgcgatacag ctccatcgcg cgcggcacgt gcggcccgtg
    13921 gccgagcagc aggtcggcgc cggcgtcgat catcgcgcgc gcgaacatca ccacatcgcc
    13981 gcgatcctcg ccggcataga actcgcgcgc gtacggcagc gcctcggcgc ccgcgccttc
    14041 gccgccggcg tggaacgaca cgatcacgat atcgttcgcc gcggcgagcg agcgcacgaa
    14101 ctcgactgcg agttcgtggt cgttgagcga gttcgcaccg acattgggcg cgaacgcgac
    14161 catcgcgacc cgcacgccct tcactgtcat cccggcccag gtgccctcgc ggcccgaatg
    14221 gcggatgccg acggcgtcga gggcggccat gctcgaatcg cgcccctcct cgccgaaatc
    14281 acgtgcgtgg ttgttcgcga gactcacgag atcgaagccc gcatcgcgca gatacgccgc
    14341 atagcgcgtc ggcgtgcgga acacgaagca gctcgtcgag gagccgcact gcttgaccgg
    14401 ctcgccgccg tccatcagca cgccctcgag gttgccgaag gcgacatccg cgccggcgag
    14461 gatcggcgtc acgtggcgca ggaagccgat cccgtcgtcg tccggcagga tgttcttcgg
    14521 gaagtcggta ccgagcatga tgtcgcccac cgcggcgatc gagatgacgg tgggcggggg
    14581 cgctggaggg ggtggcgcag cttccggggc gggcggcgat ggcgggggca ccgtcgcgca
    14641 accggcgaac agcagtgcga gtgcgagcgc gccgaggcta cgggcgtgca ggctggtcgt
    14701 gcccgaatgc gaggggctgc gccgtggctt cggctcgcag tccatcagtc cccgacccgg
    14761 atcaccagcg catcgatgtc gttggcgcgc agccgcgcac gcacgcgatt caactcggcg
    14821 agatcggcga tgggaccgat gcgcacgcgg tgccagacgt cggcgtcgac cgcgacgcgc
    14881 tgcacggccg cctggatgcc ctgcagcgcg agctgcgagc gcacgcgttc ggcatcggcc
    14941 tggccacgat aggagccggc ctgcagcacg tacacgcccg gccgctcgac cgcggcgggt
    15001 gcgtcgggct tcacttcgcg atcgcgctcc ggcaccacga cctcgaactt cggcagcatc
    15061 tcgtagaagt cgtattgcct gccggcctct tcgccgggca cttccgcgac ctgctcatcc
    15121 gcgggcgcgg ccgcgacgcg cggctcgggg cgctccagcg ccacatcccg cgcggtctgc
    15181 aacgcgcgct ggttcatcac cacgtagacg agcagcgcca cgccgaggcc ggtggcgagc
    15241 ccgaggccga actggcgcca gcgtgcgaat tcgacactgc cgctgcggga tctcttgaag
    15301 tcgcgtgccg tcagtctcat ggcgagcgca agcttagcgc gtcacatcgt ctcgggtgcg
    15361 gaaacgccga gcacgccgag gccattgcgt atcacctgct gcacgccgag cgcgagcgcg
    15421 aggcgcgcat tgcgcagtgg cggatcctcg acgatgaact gcgcggcgtt gtaccaggtg
    15481 tggaaagtgt tggcgagatc gcgcaggtag tgcacgagcg tgtgtggcgt gcggttcgcc
    15541 gccgcctgac acagcatttc cgggtagcgc accagcgccg tgagcgcggc ctgctcgtgc
    15601 tcgccggtga gcaggccgag gttcgcccgc gccacggcct cgtcgtagcg atagcccttc
    15661 gcgaccagct gcttcatcac gctcgacacg cgcgcgtgcg catactggat gtagtacacg
    15721 gggttctcgt tcgagcgcga cttggcgagc tcgaggtcga agtcgagcgg ctggtcgtgg
    15781 ctcctcatca ggtagaagaa ccggcaggcg tcggtaccga cttcctcgcg cagttggcgc
    15841 agcgtgacga actgcgcgtc gcgtttgccc atcgggatct tcacctcgcc gcgccacagg
    15901 ctgacgaact ggatcaggat cacctcgagg cagtcggcgg gctgtcccat cgcgacgagg
    15961 ccgccacgca cgcgcgcgac atagccgcgg tggtccgcgc cgagcacatc gacgagcagg
    16021 tcg
//
LOCUS       DAOQVY010000050        15203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_4429_length_15203_cov_5.608595, whole genome shotgun sequence.
ACCESSION   DAOQVY010000050 DAOQVY010000000
VERSION     DAOQVY010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15203
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4429_length_15203_cov_5.608595"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..1170
                     /gene="tgt"
                     /locus_tag="PKN91_06400"
     CDS             40..1170
                     /gene="tgt"
                     /locus_tag="PKN91_06400"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004723155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNS27737.1"
                     /translation="MTLEFELLATAGAARRGRLTLRHGVVETPAFMPVGTYGTVKAMT
                     AEELEALGADIVLGNTFHLMLRPGTEVVAAHGGLHRFMHWRRPILTDSGGFQVFSLAQ
                     LRRISEEGVRFRSPVDGSEVQLTPEISMDVQRALCSDIVMAFDDCTAYPATHEEARIA
                     MERSMRWAARCRDHYARADEAPGVLFGIVQGGMHADLRRASLDALLALELPGLAIGGL
                     AVGEPEEQRLAVLDGLVPQVPRELPRYLMGVGRPEDIVQAVLRGVDMFDCVMPTRHAR
                     NGRLFTADGDINIRNAVHQFDTGPIDPACGCYTCANYSRAYLRHLARCNEILGARLNT
                     IHNLHYYLDLMRRIRASIAAGGFEAFARDFIEARRSVTAAMA"
     gene            1211..1540
                     /gene="yajC"
                     /locus_tag="PKN91_06405"
     CDS             1211..1540
                     /gene="yajC"
                     /locus_tag="PKN91_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNS27738.1"
                     /translation="MNALIPEAYAQAAGASPGGSFAPLLMMVVFIVIFYFLLIRPQQK
                     KAKEHQAMLGRIASGDEVVTAGGILGKVVEVGDTITTIEIADGVRVKVQKFQITSLVP
                     KGTIKGA"
     gene            1550..3421
                     /gene="secD"
                     /locus_tag="PKN91_06410"
     CDS             1550..3421
                     /gene="secD"
                     /locus_tag="PKN91_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNS27739.1"
                     /translation="MLEFARWKYILVASVLLVAILFALPNVFGEDPALQVVRKDRAPM
                     DAAAEQRLAGFLDERSIQYSSTFVDSGRLMIRFDSVPMQLQARDAVNEQLKDEYITAL
                     SFASRAPTWLTRLGLRPMPLGLDLRGGLHLLYQVDVNGAVSQLLESYEQDFRRVLGEK
                     NLAFLDVVQITASAEIPNGVRVLLAPGTDAGEARDVLRELLPDLDVQSVNLASGPAVQ
                     AVLTAVQIRQRQDYAIQQNLTTLRNRVNELGVSEPIVQRQGFDRIIVQLPGVQNSADV
                     KDILGKVATLEFRLVDTQGSIAEAVQRGRAPLGSKLYRDKNGQPVLLKRELIVTGDQL
                     TDAITAATQDGPGVSVKLDARGGAQMLKTTQANLNKPMAVVFIEKSRETVEVGGKTIE
                     RDVTDQRVISVATIRGVFSNQFQITGLTQSEARELALLLRAGSLAAPIFLAEERAIGP
                     SLGAQNIEQGVKALVVGMLGVFIFMVIYYQVFGLVADLVLLANVVLLSALLSMMGAAL
                     SLPGIAGIILTVGMAVDANVLIYERIREEQRNGVSPQAAIRAGFEKAFSAIADSNVTT
                     LFAGLVLWVFGTGAIRGFAVVLSLGIATSMFTSLMGSRALLTLMYGGRTKTGKLAIG"
     gene            3445..4380
                     /gene="secF"
                     /locus_tag="PKN91_06415"
     CDS             3445..4380
                     /gene="secF"
                     /locus_tag="PKN91_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNS27740.1"
                     /translation="MEFFHKQTKIPFMGIRKVSYGLSIVLMVVSLGAILAKGLNLAID
                     FTGGVAVEAALPSAADVDAIRGKLEEAGFHEPQVGIFGSSREVSIRLPPDDSQSVTEI
                     RTRVEQALLAVEPGAEVRNLAVVGPQVGDELRTSAIWALTFTLLLIFIYIAFRFHTWR
                     LSVGAIVAVMHDPILVLGIFALTQTPFDLAVVAAILAVIGYSLNDTVVIFDRIRENFE
                     RNRRLPPMTVLDQAINQTLSRTIMTKVVTLIVVFALLFLGGPVLRGFSEALTIGILAG
                     TYSSIYISSAIALDLGLTAEHVFPTVKKTAADDMP"
     gene            4471..6276
                     /locus_tag="PKN91_06420"
     CDS             4471..6276
                     /locus_tag="PKN91_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNS27741.1"
                     /translation="MKWRTKSVDQVQAEYDRGELKRTLGPWHLVLLGIGCIIGAGIFV
                     RTGNAAALHAGPAVMVSFVFAGVVCAFAGLCYAELASMLPVSGSAYTYTYTTIGEFGA
                     WIMGALLLLEYGLAASVVAVGWSGYAMSLLNDIGLGIPAEFTQAMGKLVVAHTTTFSI
                     ADPLVSLNGVTARLTDGSVVQFLSSGSAQLPGVHQSALAIYDVLGSSAGGGFTLTNAA
                     DIHLSQDIQTALAGAVKVIDPANGHLRELAAGTQLLAPAGAIVQLPAGSVVPIAASAV
                     VTSLFNLPAVAIVLAVMLLLVIGVSESATVNNIIVAIKVAVILAFIAVGAFYVDSANW
                     RPFIPEPTGEPFEFGWSGVMRAATIVFFAYIGFEAVSTAGQEAKNPRRDMPFGILGSL
                     VICTLLYMATSAVLTGVISYTKLNVAAPVAVAADTFGPQWGWLVKAIKIGAIAGLSSV
                     ILVLMFGQTRVFYTMSRDGLLPRALAKVHPRFLTPWINTLIVGVVVALAAAVFDINTL
                     GDLTSIGTLAAFAIVCVAVMWLRRARPDLPRQFKVPFYPLTPILGILSCLFLITQVEP
                     RVLHFFTWFLAGSVVVYFLYGYWASPMRERASGKA"
     gene            complement(6313..7113)
                     /locus_tag="PKN91_06425"
     CDS             complement(6313..7113)
                     /locus_tag="PKN91_06425"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNS27742.1"
                     /translation="MQPPVNIAVRAARRAGEIIVRSMNRLESIQATAKSRNDFATEVD
                     QAAEQEIIGIISRAYPQHAFLAEESGRTGESETVWIIDPLDGTTNYIHGFPTFAVSIA
                     CQHRGRLEHAVIYDPMRQEIFSASRGDGARLENRRIRVSNRPGMEGALIATGFPFRAN
                     LEYADAYLAQLKVVMASAAGVRRPGAAALDLAYVAAGRVDGFWEFGLNAWDTAAGALL
                     IQEAGGRIGTMSGAPYDLSPHVVAGAPRVYDALLELLTPHVPDALRKA"
     gene            7155..7907
                     /locus_tag="PKN91_06430"
     CDS             7155..7907
                     /locus_tag="PKN91_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNS27743.1"
                     /translation="MRAADIRFVLVGTQHPGNIGAVARAMKNMALERLVLVRPREFPH
                     PEALSRASGADDVLAGARVFGSLAEAIGDCGYVVATTAREREHNHRVIDVRDAAARVV
                     AEAARGPVAVLFGAERAGLSNEEIDAAHALLRIPANPAYTSLNIAMAAQLIAYEIYRA
                     RGARVEASRGTVPLATPAEMARLYEHLEQVLAEINFRDRTQSGTNLMQRLRRLFQRAE
                     PDQNEVNILRGILAAVQGKRRVAGSKCPPRAS"
     gene            7914..9071
                     /locus_tag="PKN91_06435"
     CDS             7914..9071
                     /locus_tag="PKN91_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNS27744.1"
                     /translation="MGRSPVYLDNAATTAVDPRVAAAMNECLTAEGVFGNPSAAHAYG
                     REAARRVASARREVAAIVGADPASIVFTSGATEAANLALLGSARANADRRRHLVTART
                     EHRATLDPIRQLEKEGFAVTWLTPDRAGRIDPGAVRAALRPDTLYASIMHVNNETGMV
                     QDIAAIGQVCREAGVLYHCDLAQGIGKLPTTEVARHVDFGSFTAHKIHGPKGVGALYV
                     APGARPWLRPIMYGGGQEQGLRSGTIPTHQVVGFGRACAIALAEGASDSARMRELRGA
                     LWRALATLPDVLPNSALDEGSPAILNVSFAGVEGESLVAGLTDVAVSTGSACNSASGE
                     PSYVLRALGRDTELAQASLRFSLGRWTTLDDIERAAQAVRREVTRLRAVAP"
     gene            9068..9454
                     /locus_tag="PKN91_06440"
     CDS             9068..9454
                     /locus_tag="PKN91_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNS27745.1"
                     /translation="MNAPDPLSPLTRASFASLERAGRFADSAGVVSGEAGSPATGTHV
                     RFHLRLAGSRVAEARFQAYGCPHTLAVANWLAGEMAGRTREAPWPGAPPDWAARFEVP
                     VAKLGRLLVIEDALRACLAAWPETVE"
     gene            9460..9783
                     /locus_tag="PKN91_06445"
     CDS             9460..9783
                     /locus_tag="PKN91_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016537756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNS27746.1"
                     /translation="MSIQLTPAAAERVSSFLAARGKGLGLRLAVRKTGCSGFAYVVNY
                     ADEQLAGDRVFEHRGVKVFVDPESLPLVEGTTVDFVKQGLNEAFRFSNPNIKGECGCG
                     ESFSV"
     gene            9820..10248
                     /gene="ndk"
                     /locus_tag="PKN91_06450"
     CDS             9820..10248
                     /gene="ndk"
                     /locus_tag="PKN91_06450"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNS27747.1"
                     /translation="MALERTLSIVKPDGVSRNLIGEVVRRLEKAGLRVVASRMLRLSR
                     EQAEGFYAVHRERPFFNDLVRYMTSGPVVVQVLEGENAVALNREVMGATDPKKAAAGT
                     IRADLAESIEANTVHGSDSPENAAIEIAYFFKGSDICPRG"
     gene            10262..11401
                     /gene="rlmN"
                     /locus_tag="PKN91_06455"
     CDS             10262..11401
                     /gene="rlmN"
                     /locus_tag="PKN91_06455"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNS27748.1"
                     /translation="MSAGSVADERVNLLGLARPELEAFVGTLGSKPFRARQLMQWMYG
                     RGESRFEAMTDLARDFRALLATRAEIRPPEIVTAQCSADGTRKWLLRADATQAIEMVY
                     IPEENRGTLCISSQVGCALDCAFCSTAQQGFNRNLTTAEIVGQVWLAERELERERVPV
                     PGESPGQRAITNVVLMGMGEPLANFRNVVPALGIFTDDFGFDISRRRVTLSTSGLVPQ
                     IYKLAEEANVALAVSLHAPTDELRSTLVPINRRHPIAELLEACWHYIDEQNGRSVTFE
                     YVMLDGVNDQPEHARALVRLLKGRPAKLNLIPFNPFPGTQYRRSPAATILRFRDELLS
                     RGVLATIRRTRGDDIDAACGQLAGRVKDRTTVRLGNKLIGVAIQT"
     gene            11398..12168
                     /gene="pilW"
                     /locus_tag="PKN91_06460"
     CDS             11398..12168
                     /gene="pilW"
                     /locus_tag="PKN91_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HNS27749.1"
                     /translation="MKALHTIAAVATSLAALLLAGCTTTQSGPATSDSSAAEYNVQLG
                     YAYLRQGNLSVAKEKLERAAEQDPRSSAVHSALGLLYERLGDTRAADRHYSTALRLAP
                     RDPEIENNYAIYLCRNERVAEGVKRFEHAARNPLYRTPEAAYTNAGVCQRGAGLNAEA
                     EANFRRALQLRPNYAEAAYQLGDLRLQKGETDAARTDLDIYLSSFRATPELLLLRVRI
                     ARATGDRVAEEKFSRMLRMDFPDSEQTRALGTLTRNPG"
     gene            12165..12992
                     /locus_tag="PKN91_06465"
     CDS             12165..12992
                     /locus_tag="PKN91_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNS27750.1"
                     /translation="MTASDGDDLEPGIGAQLRAARERAGLSALQAAAKLHLDPRYIEA
                     LEGDDFSALGAPVYVRGHLRRYAELVNAPAAALVERYAASVEVPPPDLHPVQQTESLR
                     PSRQLAGPVIAIIVAAAVIAGIWLALKGLPSASLPREAPAPAASVAAPAEDAPAAQSG
                     ETNGSATPASDAAANADTVAPVAAAGAATRVRIAYTAECWTEIHDARNQRLYYGLGQP
                     DGIVSVQGTAPLRVLLGYAGSATVEIDGRVVPIPAEARRGRRANFVITSGGTVTALP"
     gene            12989..14263
                     /gene="hisS"
                     /locus_tag="PKN91_06470"
     CDS             12989..14263
                     /gene="hisS"
                     /locus_tag="PKN91_06470"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNS27751.1"
                     /translation="MTKAIQPVRGMNDVLPADIGLWQHLEAALRELVAAYGYEEIRVP
                     VVEHTELFRRSIGEFTDIVEKEMYTFTDQGGDSLTLRPEATAGIARAAISNGMLRGQK
                     HKLWCIGPMFRHERPQKGRYRQFHQFDIEAIGFEGPDVDAELIVMMARLWRRLGLARV
                     RLQLNSLGTPQSRAGYRDKLVQYFRGHEAALDADSRRRLGGNPLRILDSKNPDMQALI
                     KDAPLIVDHLDDASRAHFEALQATLRAAGVEFVVNPRLVRGLDYYSRTVFEWTTDALG
                     SQDAVCSGGRYDGLIAQLGGEATPAIGFAMGIERVVELLHLAGTAPAKGVPDAYIVAQ
                     GERAVSAGIALAERLRDELPRRRIEFGAGGGNFKAQFRRADRSGARFALVLGDSELER
                     GVVALKNLRGEANQIECPMSEVGTRLEAKLKE"
     gene            14267..14968
                     /locus_tag="PKN91_06475"
     CDS             14267..14968
                     /locus_tag="PKN91_06475"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS27752.1"
                     /translation="MDEFMSESEQWELVKRWVRENAIWVVGGVALGVAGLAGWRIWQE
                     RIERLAHEASDRYEQALDAFARDDRTRGFTIVDELRRDQKSSPYSDQADLLAARIHVD
                     GREYDKAVERLQRVMNDARDEELRQVARGRLARLQIQRGNADQALALLPTSGGGAFAA
                     RYEEIRGDALLAKGDKSAALTAYRAARASAASGAVDPGLLELKINALTPAEAPPAEPA
                     PAASTAEPAPTGTDG"
     gene            14968..>15203
                     /locus_tag="PKN91_06480"
     CDS             14968..>15203
                     /locus_tag="PKN91_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27753.1"
                     /translation="MRRLAIVVALLALAACSKDKEVNPPAELVDFNQTLAVERVWSAG
                     VGGGDPKLRLGLGVTVVEQQVFAAGHGGEVIAVAL"
BASE COUNT         2308 a         5182 c         5275 g         2438 t
ORIGIN      
        1 cgacgcgatg ttcgtgacgc cggcgcgcgg cgtgcgcgca tgacgctcga attcgaactg
       61 ctggcgacgg caggcgcggc gcgacgcggg cggctcacgc tgcgccacgg cgtcgtcgag
      121 acaccggcct tcatgccggt cggcacgtat ggcacggtca aggcgatgac cgccgaggaa
      181 ctcgaggcgc tcggcgcgga catcgtgctc ggcaacacct tccacctgat gctgcgcccg
      241 ggcaccgagg tcgtcgcggc gcatggcggc ctgcaccgct tcatgcactg gcggcggccg
      301 atcctcaccg actcgggcgg cttccaggtg ttcagcctcg cgcagctgcg caggatcagc
      361 gaggagggcg tgcggttccg ctcgccggtc gacggcagcg aagtgcagct gacgcccgag
      421 atctctatgg acgtgcagcg cgcgctgtgc tcggacatcg tgatggcgtt cgacgactgc
      481 accgcgtacc cggcgacgca tgaggaggcg cgcatcgcga tggagcgctc gatgcgctgg
      541 gcggcgcgct gccgcgatca ctacgcgcgg gcggacgagg cgccgggcgt gctgttcggc
      601 atcgtgcagg gcggcatgca cgccgacctc aggcgcgcat cgctcgatgc gctgctggcg
      661 ctcgagttgc cgggcctcgc catcggcggg ctcgcggtcg gcgagccgga ggagcagcgc
      721 ctcgccgtgc tcgatgggct cgtgccgcag gtgccgcgcg agctgccgcg atacctgatg
      781 ggagtcgggc gcccggagga catcgtgcag gccgtgctgc gcggcgtcga catgttcgat
      841 tgcgtcatgc cgacccggca cgcccgcaat ggccgcctgt tcacggccga cggcgacatc
      901 aacatcagga atgccgtcca ccagttcgat accgggccga tcgacccggc ctgcggctgc
      961 tacacctgcg ccaactactc gcgcgcctac ctgcgccacc tcgcgcgctg caacgagata
     1021 ctgggggcgc ggctcaacac gatccacaac ctccactatt acctggacct gatgcggcgg
     1081 atccgcgcca gcattgccgc gggcggcttc gaggccttcg cccgggactt catcgaagcc
     1141 cgtcggtcgg tgaccgcggc tatggcataa tgacaagctt taccgacccg cacccgcaag
     1201 aggtcctcaa atgaacgctt tgattccgga agcctacgcg caggccgcag gcgcgtcgcc
     1261 gggcggctcg ttcgcgccgc tgctgatgat ggtcgtgttc atcgtcatct tctatttcct
     1321 gctgatccgt ccgcagcaga aaaaggcgaa ggaacaccag gcgatgctcg ggcgcatcgc
     1381 gagcggcgac gaggtcgtga ccgccggcgg catcctcggc aaggtcgtag aggtgggtga
     1441 cacgatcacc accatcgaga tcgccgacgg cgtgcgcgtc aaggtgcaga aattccagat
     1501 cacctcgctc gtgccgaagg gcacgatcaa gggcgcctga ggaccccgga tgctcgagtt
     1561 cgcccgctgg aagtacatcc tcgtcgcgag cgtgctgctc gtggcgattc tgttcgcgtt
     1621 gccgaacgtg ttcggcgagg accccgcgct gcaggtcgtg cgcaaggacc gtgcgccgat
     1681 ggacgccgcc gccgaacagc ggctcgcggg cttcctcgac gagcgcagca tccagtacag
     1741 ctcgaccttc gtcgactcgg gccggctgat gatccgcttc gacagcgtgc cgatgcagct
     1801 gcaggcgcgt gatgccgtga acgagcagct gaaggacgag tacatcacgg cgctgtcgtt
     1861 cgcctcgcgc gcgccgacct ggctcacgcg cctgggtctg cgaccgatgc cgctaggcct
     1921 cgacctgcgc ggcggcctgc acctgctgta ccaggtggac gtgaacggcg cggtgagcca
     1981 gctgctcgag agctacgagc aggacttcag gcgcgtactc ggcgagaaga atctcgcgtt
     2041 cctcgacgtc gtgcagatca cggccagcgc cgaaattccg aatggcgtgc gggtgctgct
     2101 cgcgcccggc accgatgccg gcgaggcgcg cgacgtgttg cgcgaacttc tgccggacct
     2161 cgacgtgcag tcggtcaacc tggcgagcgg gccggccgtg caggcggtgc tcactgcggt
     2221 gcagatccgc cagcggcagg actatgcgat ccagcagaac ctgacgacac tgcgcaatcg
     2281 cgtcaacgag ctcggcgtgt ccgagcccat cgtgcagcgg cagggcttcg accgcatcat
     2341 cgtgcagctg ccgggcgtgc agaactcggc cgacgtgaag gacatcctcg gcaaagtggc
     2401 gaccctcgag ttccgcctgg tcgataccca gggcagcatc gcggaagccg tgcagcgtgg
     2461 tcgcgcaccc ctcggctcga aactctatcg cgacaagaac ggccagcccg tgctgctcaa
     2521 gcgcgaactc atcgtcaccg gcgaccagct caccgacgcg atcaccgcgg cgacacagga
     2581 cggtccgggc gtgtcggtga aactcgatgc gcgcggcggc gcgcagatgc tgaagacgac
     2641 gcaggcaaac ctgaacaagc cgatggcggt ggtgttcatc gagaagtcgc gcgagaccgt
     2701 cgaggtcggt ggcaagacga tcgagcgcga cgtgaccgac cagcgcgtga tcagcgtcgc
     2761 gacgatccgc ggcgtgttca gcaaccagtt ccagatcacc ggactcacgc agagcgaggc
     2821 gcgcgagctc gcgctgctgc tgcgcgccgg ctcgctcgcc gcgccgatct tcctcgccga
     2881 ggagcgcgcg atcggcccga gtctcggtgc gcagaacatc gagcagggcg tcaaggcgct
     2941 ggtcgtcggc atgctcggcg tgttcatctt catggtgatc tactaccagg tcttcggcct
     3001 ggtggccgac ctggtgctgc tcgccaacgt ggtgctgctg tccgcgctgc tgtcgatgat
     3061 gggcgcggcg ctgtcgctgc cgggcattgc cggcatcatc ctgacggtcg gcatggcggt
     3121 cgacgccaac gtgctgatct acgagcgcat ccgcgaggag cagcgcaacg gtgtctcgcc
     3181 gcaggctgcg atccgcgccg gcttcgagaa ggcgttctcg gcgatcgccg actcgaacgt
     3241 caccacgctg ttcgccggcc tcgtgctgtg ggtgttcggc accggcgcga tccgcggctt
     3301 cgcggtcgtg ctgtcgctcg gcatcgcgac ctcgatgttc acttccctta tgggcagccg
     3361 tgcgctgctt accctgatgt acggcggccg cacgaaaacc ggcaagctcg cgatcggctg
     3421 acggcaagag gctaggaccg acacatggag tttttccaca agcagaccaa gattccgttc
     3481 atgggcatcc gcaaggtgtc ctacggattg tccatcgtcc tgatggtggt cagcctcggc
     3541 gcaatactcg cgaagggcct gaacctcgcg atcgacttca ccggcggcgt ggcggtcgag
     3601 gcggcgctgc cgtccgcggc cgacgtcgat gcgatccgcg gcaagctcga ggaggcaggc
     3661 tttcacgagc cgcaggtcgg catcttcggc tcctcgcgcg aagtgtcgat acgcctgccg
     3721 cccgacgatt cgcagtcggt caccgagatc cgcacgcgcg tcgagcaggc cctgctcgcc
     3781 gtcgagcccg gtgcggaggt gcgcaacctc gccgtggtcg ggccgcaggt cggtgacgaa
     3841 ctgcgcacca gcgcgatctg ggcgctcacg ttcacgctgc ttctgatctt catttatatc
     3901 gccttccgct tccacacctg gcggctctcg gtaggcgcga tcgtcgcggt gatgcacgac
     3961 ccgatcctgg tgctcggcat cttcgcattg acgcagacgc cgttcgacct cgcggtggtg
     4021 gcggcgattc tcgccgtgat cggctactcg ctgaacgata ccgtcgtgat cttcgaccgc
     4081 atccgcgaga acttcgagcg caaccggcgc ctgccgccga tgaccgtgct cgaccaggcg
     4141 atcaaccaga cgctctcgcg cacgatcatg accaaggtgg tgaccctgat cgtcgtgttc
     4201 gcgctgctgt tcctcggtgg cccggtgctg cgtggcttct ccgaggcgct gacgatcggc
     4261 atcctggcgg gcacctattc gtcgatctac atttcctctg cgatcgcgct cgatctcggg
     4321 ctgacggccg agcacgtgtt cccgaccgtc aagaagaccg cggccgacga catgccctga
     4381 gtaccgccgc gggcgccgca cccgaggatg acgccggcga cgcatgtgca tatgatgcgt
     4441 ccgccgacag acaataacgg gggttggcta atgaaatggc gcacgaaatc cgtcgaccag
     4501 gtccaggctg aatacgatcg cggcgagctg aagcgcacgc tcggcccgtg gcacctcgtg
     4561 ctgctcggca tcggctgtat catcggcgcc ggcatcttcg tgcgcacggg caacgctgcg
     4621 gcgctgcacg cgggccccgc ggtgatggtc tccttcgtgt tcgccggagt cgtctgcgcc
     4681 ttcgcgggcc tctgctacgc cgagctcgct tcgatgctgc cggtctcggg ctccgcctat
     4741 acctacacct acacgacgat cggtgagttc ggcgcctgga tcatgggtgc gctgctgctg
     4801 ctcgaatacg gtctggcggc ctcggtcgtc gcggtcggct ggtccggcta tgcgatgagc
     4861 ctgctgaacg acatcggcct cggcattccg gccgaattca cccaggccat gggcaagctc
     4921 gtggtcgcgc acacgaccac cttcagcatc gccgatccgc tcgtgagcct gaatggcgtc
     4981 acggcgcgcc tcaccgacgg ctcggtcgta cagttcctct catcgggcag cgcgcagctg
     5041 ccgggcgtgc accagtcggc gctcgcgatc tacgacgtcc tcggcagcag cgcgggcggc
     5101 ggcttcacgc tgaccaatgc cgccgacatc cacctctcgc aggatatcca gacggcgctc
     5161 gcgggcgccg tcaaggtgat cgacccggcg aacggccacc tgcgcgagct ggccgccggg
     5221 acacagctgc tcgcgcccgc cggggcgatc gtgcagttgc cggccgggtc ggtggtgccg
     5281 atcgcggcga gtgccgtcgt gacctcgctg ttcaacctgc cggcggtcgc gatcgtgctc
     5341 gcggtcatgc tgctgctcgt gatcggcgtc tccgagtcgg ccaccgtcaa caacatcatc
     5401 gtcgcgatca aggtggcggt gatcctcgcc ttcatcgcgg tcggcgcgtt ctatgtcgac
     5461 agcgcgaact ggcgcccgtt cattcccgaa ccgaccggcg agcccttcga gttcggctgg
     5521 agcggcgtga tgcgcgcggc cacgatcgtg ttcttcgcct atatcggttt cgaggcggtg
     5581 tcgaccgccg gccaggaggc gaagaatccg cgccgcgaca tgccgttcgg cattctcggg
     5641 tcgctggtca tctgcaccct gctgtacatg gcgacttcgg ccgtgctcac cggcgtcatt
     5701 tcctacacga agctcaatgt cgcagctccg gtcgcggtcg cggccgatac cttcgggccg
     5761 cagtggggct ggctcgtcaa ggcgatcaag atcggcgcga tcgcgggcct gtcctcggtg
     5821 atcctcgtgc tgatgttcgg ccagacgcgc gtcttctata cgatgtcgcg cgacggactg
     5881 ctgccgcgtg cgctcgcgaa agtgcacccg cgcttcctga cgccgtggat caacacgctg
     5941 atcgtcggcg tggtggtggc actcgccgcg gcggtgttcg acatcaacac gctcggcgac
     6001 ctgacttcga tcggcacgct cgccgcgttc gcgatcgtgt gcgtcgcggt catgtggctg
     6061 cgtcgcgcgc gacccgacct gccgcgccag ttcaaggtgc cgttctatcc gttgacgccg
     6121 atcctcggca tcctctcgtg cctgttcctg atcacgcagg tcgagccgcg cgtgctgcac
     6181 ttctttacct ggttcctcgc gggctcggtg gtcgtgtact tcctgtacgg ctactgggcg
     6241 agcccgatgc gcgagcgcgc gagcgggaag gcgtagagcg ggacccgcgc gggccccggc
     6301 cccgagcggg gctcaggcct tgcgcagcgc gtcgggtacg tggggtgtga gcagctcgag
     6361 cagcgcgtcg tagacgcgcg gcgcgcccgc gaccacgtgg ggagacaggt cgtaaggcgc
     6421 acccgacatc gtgccgatgc ggcctcccgc ctcctggatc agcagggcgc cggctgcggt
     6481 gtcccacgcg ttcaggccga actcccagaa cccgtcgaca cgccccgcgg cgacataggc
     6541 aaggtcgagc gccgcagcgc ctggccggcg cacgccggcc gccgatgcca tgacgacttt
     6601 cagctgcgcc agataggcgt cggcatattc caggttggcg cgaaacggaa agccggtggc
     6661 gatcagcgcg ccttccatgc cggggcgatt gctgacccgg atgcggcggt tctcgaggcg
     6721 cgcgccatcg ccgcggctcg cggagaagat ctcctgacgc atcgggtcgt agatcacggc
     6781 atgctcgagc cggccgcgat gctggcaggc gatcgagacg gcaaaggtcg gaaagccgtg
     6841 gatgtagttg gtcgtgccgt cgagcggatc gatgatccac acggtctcgg actcgccggt
     6901 gcggccgctc tcctcggcga ggaaagcatg ttgcgggtag gccctggaga tgatgccgat
     6961 gatctcctgc tcggcggcct ggtcgacttc ggtggcgaag tcgttgcggc tcttggcggt
     7021 cgcctggatg ctttcgagcc gattcatgct gcgaacgatg atttcgccgg cgcggcgcgc
     7081 agcgcggacg gcgatgttga cgggtggctg catgttgctc gaggctcgac ggggaaggca
     7141 tttacaatac cacgatgcgt gcggcggaca tcaggttcgt gctggtcggc acccagcacc
     7201 ccggcaacat cggcgcggtg gcgcgcgcga tgaagaacat ggcactcgag cggctcgtgc
     7261 tggtgcggcc gcgcgagttc ccgcatccgg aggccttgtc gcgcgcgtcc ggtgccgatg
     7321 acgtgctcgc cggcgcccgg gtcttcggca gtctcgccga ggcgatcggc gattgcggtt
     7381 atgtcgtcgc caccaccgcc cgcgagcgcg aacacaacca ccgcgtgatc gacgtgcgcg
     7441 acgccgccgc gcgcgtcgtc gccgaggcgg cgcgcggtcc ggtcgcggtg ctgttcggcg
     7501 ccgagcgcgc gggcctgtcc aacgaggaaa tcgatgcggc gcacgcgctg ctgcgcattc
     7561 cggcgaatcc cgcctacacc tcgctcaaca tcgcgatggc cgcgcagctg atcgcctacg
     7621 agatctaccg cgcgcgcgga gcgcgcgtcg aggcttcgcg tggcaccgtg ccgctcgcca
     7681 cgccggcgga gatggcgcgc ctctacgagc acctcgaaca ggtgctcgcc gaaatcaact
     7741 tccgcgatcg cacccagagc ggcaccaacc tgatgcagcg cctgcggcgc ctgttccagc
     7801 gcgccgagcc ggaccagaac gaagtaaaca tcctgcgcgg catactcgcc gcggtgcagg
     7861 gcaagcggcg tgtcgcgggc agcaagtgcc cgccgcgcgc ctcgtgacgg cgcatgggcc
     7921 gctctcccgt ctacctcgat aacgccgcga ccaccgccgt cgacccgcgc gtcgcggcgg
     7981 ccatgaacga gtgcctcacc gccgagggcg tgttcggcaa cccgtcagcg gcccacgcct
     8041 acggacgcga ggcggcgcgg cgtgtcgcga gcgcacggcg cgaggtcgcg gccatcgtcg
     8101 gcgcggatcc ggcctcgatc gtgttcactt ccggggccac cgaggcggcc aatctcgccc
     8161 tgctgggcag cgcgcgcgcg aatgcggacc ggcgccggca cctggtcacc gcgcgcaccg
     8221 aacatcgcgc gacgctcgat ccgatccgcc agctcgagaa ggaaggcttt gcggtcacct
     8281 ggctgacgcc ggaccgggcg gggcgcatcg atccgggtgc cgtgcgcgcg gcactgcggc
     8341 ccgacacgct ctacgcctcg atcatgcacg tgaacaacga gaccggcatg gtgcaggaca
     8401 tcgccgcaat cgggcaggtc tgtcgcgagg cgggcgtgct ctatcactgc gatctcgcgc
     8461 agggcatcgg caagctgccc accacggagg tcgcccggca cgtcgacttc ggctcgttca
     8521 cggcgcacaa gattcacggg ccgaaggggg tcggcgcgct gtacgtcgcg ccgggcgccc
     8581 gcccgtggct gcgcccgatc atgtatggcg gcggacagga gcagggcctg cgttcgggca
     8641 cgatcccgac gcaccaggtc gtgggcttcg gtcgcgcctg cgcgatcgcg ctcgccgagg
     8701 gcgcaagcga cagtgcgcgc atgcgcgagc tgcgcggcgc gctgtggcgc gcgctcgcca
     8761 cgctgccgga cgtgctgccg aacagcgcgc tcgacgaggg ctcgcccgcg atcctcaatg
     8821 tctccttcgc gggcgtcgag ggtgagagtc tcgtcgcagg cctcaccgac gtcgcggtct
     8881 cgacgggctc ggcctgcaat tcggcgagcg gcgagccttc ctacgtcctg cgggcgctcg
     8941 ggcgcgacac cgagctcgcg caggcttcgc tgcgtttcag cctgggccgc tggacgaccc
     9001 tggacgacat cgagcgtgcc gcgcaggccg tgcggcgcga ggtgactcgc ctgcgagccg
     9061 tggccccgtg aacgcccccg atccgctctc gccactcacg cgcgcgtcgt tcgcgtcgct
     9121 cgagcgggca gggcgttttg ccgattccgc cggggtggtc agcggggagg ccggcagtcc
     9181 cgcgaccggt acccacgtgc gcttccacct gcgcctcgcc gggtcgcggg tcgccgaggc
     9241 gcgattccag gcctacggct gcccgcatac gcttgcagtg gcgaactggc tggccggcga
     9301 aatggccggc cgtacccggg aggccccctg gcccggcgct cccccggact gggccgcccg
     9361 tttcgaggtg ccggtggcga agctgggccg gctgctcgtc atcgaggatg ccctgcgggc
     9421 ctgcctcgcc gcctggccgg agacggtaga atgatgccca tgagcatcca actcacaccg
     9481 gcggcggcgg agcgtgtttc gagctttctc gcggcccgcg gcaagggtct tggcctgcgc
     9541 ctcgcagtgc gcaagacggg ctgttcgggg ttcgcctacg tggtcaacta cgccgacgag
     9601 cagctggcgg gcgaccgggt tttcgagcac cggggggtca aggtcttcgt cgatccggag
     9661 agcctgccgc tggtcgaggg cacgaccgtg gatttcgtca agcagggact caacgaggcg
     9721 ttccggttct cgaaccccaa tatcaagggc gaatgcggct gcggcgagag ctttagcgtg
     9781 taaacttgtt ggtttgcagg cttaaagtga ggatttcgca tggcgctcga gcgcactctg
     9841 tccatcgtca agcccgacgg cgtatcccgt aacctgatcg gcgaagtcgt tcgccgtctc
     9901 gagaaggccg gcctcagggt cgtcgccagc cgcatgctgc gcctgtcgcg cgagcaggcc
     9961 gagggcttct acgcagtgca ccgcgagcgt ccgttcttca acgacctcgt gcgctacatg
    10021 acttcgggtc ccgtggtcgt gcaggtgctc gagggcgaga atgccgttgc cctgaaccgc
    10081 gaagtgatgg gcgccaccga tccgaagaag gccgcggccg gcacgatccg cgccgacctt
    10141 gccgagagca tcgaggcaaa caccgtgcac ggttcggatt ccccggagaa cgcggccatc
    10201 gagatcgcct atttcttcaa gggctcggac atctgtccga ggggctgaag gccgaagtga
    10261 catgtccgcc gggtcggtag ccgatgagcg cgtgaacctg cttgggctgg cacggcccga
    10321 gctggaggcg ttcgtcggta cgctcggcag caagccgttc cgtgcccgcc agctgatgca
    10381 gtggatgtat ggtcgcggcg agtcgcgttt cgaggccatg acggatctcg ccagggactt
    10441 ccgcgcgctg ctcgccacgc gcgccgagat ccgcccgccc gagatcgtca ccgcgcagtg
    10501 ctcggccgac ggcacgcgca aatggctgct gcgcgccgat gcgacccagg cgatcgagat
    10561 ggtgtacatc ccggaggaaa accgcgggac cctgtgcatc tcatcgcagg tcggttgcgc
    10621 gctcgactgc gcgttctgct cgaccgcgca gcagggtttc aatcgcaatc tcaccacggc
    10681 cgagatcgtc ggccaggtgt ggctcgccga gcgtgaactc gagcgcgagc gcgtaccggt
    10741 gccgggggag tcgcccgggc agcgcgcgat caccaatgtc gtgctgatgg gcatgggcga
    10801 gccgctcgcg aatttccgga acgtcgtgcc ggcgctcggg atattcaccg acgatttcgg
    10861 attcgacatc tcgcgccggc gcgtgactct gtcgacctcg gggctcgtgc cgcagatcta
    10921 caagctcgcc gaggaagcca atgtcgcgct cgccgtctcg ctgcacgcgc cgaccgacga
    10981 gctgcgcagc acgctcgtgc cgatcaatcg ccggcacccg atcgcggagc tgctcgaggc
    11041 gtgctggcac tatatcgacg agcagaacgg ccgcagcgtg accttcgagt atgtgatgct
    11101 cgatggcgtg aacgaccagc ccgagcacgc gcgcgcgctc gtgcgcctgc tgaaggggcg
    11161 gccggcaaag ctcaacctga ttccgttcaa cccgttcccg ggcactcagt accgccgctc
    11221 gccggcggcg acgatcctgc gctttcgcga cgagctgctc tcgcgcggag tgctcgcgac
    11281 gatccgtcgc acgcgcggcg acgacatcga tgcggcctgc ggacaactcg ccggccgcgt
    11341 caaggaccgc acgaccgtgc gcctcggcaa caaactgatc ggtgtggcga tacagacatg
    11401 aaggcactgc ataccatcgc ggccgtggcg acctcgcttg cggcactgct gctcgcgggc
    11461 tgcaccacga cccagagcgg gccggccacc tcggattcga gcgccgcgga atacaacgtg
    11521 cagctcggct acgcctacct gcgtcagggc aatctgtcgg tcgccaagga gaaactcgag
    11581 cgcgccgcgg agcaggaccc gcgcagttcc gcggtgcaca gtgcgctcgg cctgctctac
    11641 gaacgactgg gcgatacccg ggccgcggac aggcattact cgacggccct gcgacttgcg
    11701 cccagggatc ccgagatcga gaacaattac gcgatctacc tgtgtcgcaa cgagcgcgtc
    11761 gccgagggcg tgaagcggtt cgagcacgcg gcgcgcaatc cgctctaccg cacgcccgag
    11821 gccgcctaca cgaacgccgg cgtgtgccag cgcggtgccg gcctgaatgc cgaggccgag
    11881 gccaatttcc ggcgggcgct gcagctgcgg cccaactacg ccgaggcggc ctaccagctc
    11941 ggtgacctgc gcctgcagaa gggtgagacc gacgcggcgc ggaccgacct cgatatctac
    12001 ctgtcaagct tccgcgcgac tcccgaactg ctgctgctgc gcgtgcgcat cgcgcgtgcc
    12061 actggcgacc gggtggccga ggagaagttc tcgcgcatgc tgcgcatgga tttcccggac
    12121 tccgagcaga cacgcgcgct cgggaccctg acgcgcaatc ccggatgact gcgagcgacg
    12181 gcgatgacct ggagccgggg atcggcgcgc agctgcgtgc cgcacgcgag cgcgcggggc
    12241 tgtcggcgct gcaggccgcc gccaagctgc atctcgaccc gcgctacatc gaggcgctcg
    12301 aaggcgacga tttcagcgcg ctcggtgcgc cggtctatgt gcgcggccat ctgcgccgct
    12361 acgccgaact cgtgaacgct cccgcggctg cgctcgtcga gcgctacgcg gcgagcgtgg
    12421 aagtgccgcc gcctgacctt caccccgtcc agcaaaccga atcgctgcgg ccgtcgcggc
    12481 agctcgccgg tccggtcatc gcgatcatcg tcgccgcggc ggtgatcgcc ggcatctggc
    12541 tcgcgctgaa aggtctgccg tccgcctccc tgccgcgcga agcgcccgcg ccggccgcca
    12601 gcgtcgccgc acccgccgaa gatgcgcctg cggcgcagtc cggtgagaca aacgggtctg
    12661 ccaccccggc gtccgacgcc gccgcgaacg ccgacacggt ggcgcccgtc gcagccgcgg
    12721 gtgccgcgac gcgggtgcgc atcgcctaca ccgccgagtg ctggaccgag atccacgatg
    12781 cgcgcaacca gcgtctctac tacggcctcg ggcagccgga cggcatcgtg tccgtgcagg
    12841 gcacggcgcc gctgcgcgtg ctgctcggat atgcgggctc cgcgaccgtc gagatcgatg
    12901 gccgcgtggt gccgatcccg gccgaggcgc gccgcgggcg ccgcgcgaat ttcgtgatca
    12961 cctccggcgg cacagtgacg gcattgccat gacgaaagct atccagcccg tgcgcggtat
    13021 gaacgatgtg ttgccggccg acatcggcct gtggcagcac ctcgaggccg cgctgcgcga
    13081 gctcgtggcg gcatacggct acgaggaaat ccgcgtgccc gtggtcgagc acaccgagct
    13141 gttcaggcgc tcgatcggcg agttcaccga catcgtcgag aaggagatgt acaccttcac
    13201 cgaccagggc ggcgacagcc tcacgctgcg gccggaggcc accgccggca tcgcgcgggc
    13261 cgccatctcg aacggcatgc tgcgtggcca gaagcacaag ctgtggtgca tcgggccgat
    13321 gttccggcac gagcgcccgc agaagggccg ctaccgccag ttccaccagt tcgacatcga
    13381 ggcgatcggt ttcgaaggtc ccgacgtgga cgccgagctg atcgtcatga tggcgcgcct
    13441 gtggcgcagg ctcggtctcg cgcgggtgcg gctgcagctc aattcgctcg gcacgccgca
    13501 gtcgcgcgcc gggtaccgcg acaagctcgt gcagtatttc cgcggccatg aggcggcgct
    13561 cgacgccgac agccgccgcc ggctcggtgg caacccgctg cgtatcctcg acagcaagaa
    13621 cccggacatg caggcgctca tcaaggatgc gccgctgatc gtcgatcacc tggatgacgc
    13681 ctcgcgggcg catttcgagg cgctgcaggc cacgctgcgc gcggcgggtg tcgaattcgt
    13741 ggtcaacccg cggctcgtgc gcggcctcga ttactattcg cgcacggtgt tcgagtggac
    13801 caccgacgca ctgggttcgc aggacgcggt gtgctcgggc ggacgctacg atggcctgat
    13861 cgcgcaactc ggcggcgagg cgaccccggc gatcggcttc gcgatgggga tcgagcgcgt
    13921 agtcgagctg ctgcacctcg cgggcacggc acccgcgaag ggtgtgccgg atgcctacat
    13981 cgtcgcgcag ggcgagcggg cggtcagcgc cggcatcgcg ctcgccgagc ggctccgcga
    14041 cgaattgccg cggcgacgca tcgaattcgg tgcgggcggc ggcaatttca aggcccagtt
    14101 ccgccgcgcc gatcgcagcg gcgcgcggtt cgcgctcgtg ctcggcgaca gcgagctcga
    14161 gcgcggcgtg gtggcgctca agaatcttcg cggcgaggcg aaccagatcg aatgcccgat
    14221 gtccgaggtg ggtacccggc tcgaggcgaa attgaaggag taggcggtgg acgaattcat
    14281 gtcggagagc gagcagtggg agctcgtcaa gcgctgggtg cgcgagaacg ccatctgggt
    14341 cgtcgggggc gtcgcgctcg gcgtggcggg ccttgccggc tggcggatct ggcaggagcg
    14401 catcgagcgc ctggcccacg aggcgagtga ccgctacgag caggcgctcg acgccttcgc
    14461 ccgcgacgat cgaacgcgcg gcttcacgat cgtcgatgaa ctgcggcgcg accagaagtc
    14521 ctcgccgtat tcggaccagg ccgacctgct cgcggcgcgc atccacgtcg acgggcgtga
    14581 atacgacaag gcggtcgagc gcctgcagcg ggtgatgaac gacgcgcgcg acgaggaact
    14641 gcggcaggtg gcgcgggggc gcctggcgcg cctgcagatc cagcgcggca atgcggacca
    14701 ggcgctcgcg ttgctgccga ccagtggtgg cggggcattt gcggcccgct acgaggagat
    14761 ccgcggcgat gcgctgctcg ccaagggcga caagtcggcg gccctcacgg cctatcgtgc
    14821 cgcgcgcgcc agcgccgcca gcggtgccgt ggatcccgga ctgctcgagt tgaagatcaa
    14881 tgcgctcacg cccgcggagg cgccgccagc cgagccggca ccggcggcga gcaccgcgga
    14941 gcccgcgccg acagggacgg acggctgatg cgccgcctcg ccattgtggt tgcgctgctc
    15001 gcgcttgcgg cctgctcgaa ggacaaggag gtcaaccctc cggccgagct ggtcgatttc
    15061 aaccagacgc tcgccgtcga gcgggtatgg agcgccggcg tgggcggcgg cgatccgaag
    15121 ctgcggctcg gcctcggcgt taccgtggtc gagcagcagg tgtttgccgc gggtcacggg
    15181 ggtgaggtca ttgcggtcgc gct
//
LOCUS       DAOQVY010000051        15192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_4436_length_15192_cov_5.408205, whole genome shotgun sequence.
ACCESSION   DAOQVY010000051 DAOQVY010000000
VERSION     DAOQVY010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15192
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4436_length_15192_cov_5.408205"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..3008)
                     /locus_tag="PKN91_06485"
     CDS             complement(114..3008)
                     /locus_tag="PKN91_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HNS27754.1"
                     /translation="MSLALIVLLPFVGSLCAAFLPSNARNAEAWLAGAVALVCTGLTI
                     SLYPDIAEGGVVRLQLPWLAQAGLEFYLRMDGFAWMFTLLVSLMGALVVLYARYYMSP
                     EDPVPRFFSFLLAFMGSMLGIVLSGNLIQLVVFWELTSFSSFMLIAYWYHRVDARQGA
                     RMALTVTATGGFCLLAGVIMLGEIAGGYDLDHVLAAGSRVAAHPWYPAMLVLILLGAL
                     TKSAQFPFHFWLPHAMAAPTPVSAYLHSATMVKAGVFLLARLWPVLAGTDLWFALVCG
                     AGLASLLLGAAAALFQHDMKGVLAYSTISHLGLVTLLLGMNSRLALVAAVFHMMNHAT
                     FKASLFMAAGIVDHETGTRDLKRLGGLALAMPITATVATVAAAAMAGVPLLNGFLSKE
                     MFFAETIFAGGGPVLRYGLPAIATVAGMFSVAYSLRLIHQVFFGPPAQDLPRTPHEPT
                     RGMLLPSMLLVLACLLVGILPMRTIGPLLDIAVTSILGIDTPEYSLAVWHGLTLPLLM
                     SVIAMLGGLGIYLVRWFYGDRAVGRAPVRPPLDGRRAFDILIIVIHRSAERMVRFASS
                     RRLQPQLLMIICVALAVGWIPIAATGWTRGQEALTPIDPVYALLWVVGCSCAIGAAVQ
                     AKFHRLAALIMMGGAGLVTCLTFAWFSAPDLALTQVAVEVVTLVLLLLGLRWLPRRLM
                     FDDARRQTLRARARRGRDLLVAIVVGGGLAAISYAVLTRPRVDGLARFFVERALTEGG
                     GRNIVNVILVDFRGFDTFGEITVVSIVALTVYALLRRFRPAPESLTVPRAQREEIAHG
                     GYRSFDPLAPLPSGDMRISAVLVRLLLPMAGLVAIFLLLRGHNAPGGGFVGGLVMATA
                     FITQYMVGGAMWVEWRMRVHPQYWVALGLVSAGGAGMLAWAFGRPFLKSLAYDLHLPL
                     LGDVHLATVLLFDLGVYLVVVGSTMLILVSLAHQSFRSPREPVEPRPAEG"
     gene            complement(3039..3995)
                     /locus_tag="PKN91_06490"
     CDS             complement(3039..3995)
                     /locus_tag="PKN91_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HNS27755.1"
                     /translation="MAIRLPTRGPFHLEATVRVLQRRPANRVDVWSDGCYRRVIDTGE
                     GPLLAEVVDRGTVAAPDLRCTLHGPHSASARHTAARALRRVLGLDLDPVVLRRAAERE
                     PAMRATARALCGLRPPRFGGLFEAFASVVPYQQVSIDAGIAVLGRTVERFGDALVHEG
                     ERYYAFPTPAAIVRARPRALRACGLSAQKTRTLQMIARAIDSGALDERELDRLSSEAA
                     GARLVALRGVGPWTAGLVLLRGLGRLDVFPPGDVGAARSLRALLGTTSTAKIDATIER
                     FGTLRGYLYFCALGGQLLTRGLINPPRRGAKPVVKIRTYPRS"
     gene            complement(4003..4350)
                     /locus_tag="PKN91_06495"
     CDS             complement(4003..4350)
                     /locus_tag="PKN91_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HNS27756.1"
                     /translation="MIRIKRVYDARERGDGERFLVERLWPRGVRKEALDGVPWLKDVA
                     PSAALRSWYGHRVERWDEFRERYRKELAANRAACEPLLAASRRGPVTLLYSARDLEHN
                     SALVLRDFLREQR"
     gene            complement(4347..7205)
                     /locus_tag="PKN91_06500"
     CDS             complement(4347..7205)
                     /locus_tag="PKN91_06500"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNS27757.1"
                     /translation="MAKARRSSLDTRRPLDLPAGPQRWFHSLPELEKLGVGRISRLPA
                     SLRVLLESVLRNADGRRIRDEDVEALARWEPQARRDAEVPFVVGRVLLQDFTGVPLLV
                     DLAAMRAAVQRRGRDITRVQPLVPVDLVIDHSVQVDRYGRDDALQFNMDMEFRRNHER
                     YQFLKWGAQAFDGLRIVPPGFGICHQVNLEYLAQGVLARGDTLYPDSLVGTDSHTPMI
                     NGLGIVGWGVGGIEAEAAMLGQPVYLLLPDVVGVHLHGRLPSGATATDLVLRITQLLR
                     DAHVVGKFVEFHGEGAASLPVPDRATIGNMSPEYGATIGYFPVDEESCRYLLATGRSA
                     EQVETVRRYYRAQGMFGMPLAGECDYTTLIDLDLATVEPAVAGPKRPQDRIVLGGLKT
                     EFLSLLTGANGYGKPQTEVGEHFEVLGEGGEPRPGGGRQTGGISDPRPRNTNMRTELE
                     MMDNRPTPNRVPAPGRDGRGRGEGAVAAQLAHGDVVIAAITSCTNTSNPAVMLAAGLL
                     AKKAVELGLTVPPWVKTSLAPGSRVVSEYLAQTGLQAYLDRLGFNIVGFGCTTCIGNS
                     GPLDREIEETIARHDIIAASVLSGNRNFEARIHQSVKANFLMSPPLVVAFAIAGRVDI
                     DLTREPLAEVDGRGIYLRELWPTLEEIEALRGHAADPAVYRRIYGDFARRNPLWNAIP
                     SGSGQLYDWDPASTYIQEPPYFHASMAAYPAGTIKGARALAIFGDSITTDHISPAGAI
                     KPGSPAGEYLQARGVAIEDLNSYGARRGSHDVMVRGTFANIRIRNQMVPGTEGGETRH
                     QPGGEVLSIYDAAERYRAEGVPLLVIAGSDYGAGSSRDWAAKGARLLGVRAVIARSFE
                     RIHRSNLIGMGVLPCQLPEGTTAQSLGLDGSEVFEIELANRLRPRVAATLHVLRVGGE
                     RVSVPLTVRIDTPIEVAYYEAGGILPYVLGQLIGDEAG"
     gene            complement(7251..8117)
                     /locus_tag="PKN91_06505"
     CDS             complement(7251..8117)
                     /locus_tag="PKN91_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNS27758.1"
                     /translation="MKKLLCATDLSKRSDRAMRRAANLAQQHGAGLLLLHVVDDDQPH
                     HIIEARQAEARAHLEAQAASLPGGHGAIAAIEVVAGNAFESIARYAREWNAELIVMGA
                     HRRRALADVFIGSTLERVLRADGVPVLVVNTDEHGPYRDVLLALDVSPASARAVQAAR
                     ELGLLDGDISVVHAFDAVFTGALEGSGASAEEVRAYRDAWEREARARLRAFLATGDLA
                     DRRVELTVDSGSPFDAIHQAVLQQEPQLLVVGTRGLTGLKRVFLGSVADRVLREIECD
                     ILAVPPPRAAQR"
     gene            complement(8114..9196)
                     /gene="ald"
                     /locus_tag="PKN91_06510"
     CDS             complement(8114..9196)
                     /gene="ald"
                     /locus_tag="PKN91_06510"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNS27759.1"
                     /translation="MRIGVPREIKNHEYRVGLVPDAVRRLTEAGHTVTVETGAGAGIG
                     ISDSDYAAAGAGIASSAAETFGEAELIVKVKEPQLEECRRLTPKHVLFTYLHLAADRA
                     QAEALMASGCTAIAYETVVGADGSLPLLTPMSEVAGRMSVQVGAQYCTKALGGRGVLL
                     GGVPGVAPARVVIIGGGVSGSHAAEVAIGMRADVTILERSVPRLRELDTRFEGRARIL
                     FSTPESILAALARADLLIGAVLIPGAAAPRVVTRAMLRAMGAGAVAVDIAIDQGGCLE
                     TSRPTSHADPTYTEEGVTHYCVTNMPGAVPRTSTFALNNVTLPYVERLATLGWREATA
                     RDPGLHAGLNVVAGRIVHPAVERALQ"
     gene            9302..9760
                     /locus_tag="PKN91_06515"
     CDS             9302..9760
                     /locus_tag="PKN91_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNS27760.1"
                     /translation="MDSTDRRLLAALQRDGRATNAALAAQVGLSESACLRRMRRLEET
                     GVIRGYGARIDAGAVGYTVRVLVRITLKSQTDRDFNAFERAVQAVPNVIDCFLTTGQW
                     DYELIAIARDAADLERLHSTVLTKLPGVARVESSFVLRHVVRQVSLPLDR"
     gene            complement(9672..10148)
                     /gene="rnk"
                     /locus_tag="PKN91_06520"
     CDS             complement(9672..10148)
                     /gene="rnk"
                     /locus_tag="PKN91_06520"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HNS27761.1"
                     /translation="MRQNPILVSEADADRLRALLGAQDDSAHDQEHLHQLKAELERAV
                     VRLADEVPADVITMNARVRVRDVVTGREDAYTLVLPSEADIAARRISVLAPLGTALLG
                     YREGDEVEWRMPGGMRRLLILQVTREPVSDRGAATPGAPRGAEQNSIPHAPPRVVS"
     gene            10242..11177
                     /locus_tag="PKN91_06525"
     CDS             10242..11177
                     /locus_tag="PKN91_06525"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNS27762.1"
                     /translation="MTGKPKRLNYQHLYYFWTVVRCGSLAQASAELNLSMPTISAQLR
                     TLEERLGVELLVKAGRRLVPTDAGRTAFRYAEDIFRLGRELTDALTPGGTAQPLRLAV
                     GIDDVLPKEIAERLLAPALGQGRALRLICQEGTLERLTAALAVNDLDLALSDAPVPPR
                     LGIRAHSHRLGSCGVSWMGARALAGKLRAHFPASLDGAPVLLPTDDTAIRRALDQWFE
                     RHKLRPIVAGEFEDYALLREFARAGTGLIPVPDVMVRLYQREAGLALLGPARSVEAEF
                     YGVTLHRDISHPAVAEICAQAPLLFSRGAGPRVVS"
     gene            complement(11143..12321)
                     /locus_tag="PKN91_06530"
     CDS             complement(11143..12321)
                     /locus_tag="PKN91_06530"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNS27763.1"
                     /translation="MRTDSRFVPFAYGFRPFFLVAGLYAVIAIAGWVWIYATGRVPSA
                     ALPPLLWHGHEMLFGFIGAAIAGFLLTAVPSWTGTRGFAGPPLVALTVLWLAGRIAFA
                     ATGSLPPLWLAVCELSFLPLLAFLIGRTLLRTHNRNFPMLIIIAVLWAIDAWFLYSIT
                     NGEFALASRALRVGIGLVLLLVTVIGGRIVPNFTASALRQRGIEAAIRSRPPVERITI
                     GSMVLMVVVDLVAPSHWAAWAVAGVAALAQAVRMSGWRSLRTLSDPIVWVLHAAYAWL
                     PIGLALKAVHIATGAAWAAHWQHALTIGVAATMILAVTTRASLGHTGRPLVVARSIAV
                     AYMLLLLAALVRVFGPAVMPDYLLTIEAAALLWIAAFGIYVFVYAPILTKPRVDQRPG
                     "
     gene            complement(12377..12991)
                     /locus_tag="PKN91_06535"
     CDS             complement(12377..12991)
                     /locus_tag="PKN91_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HNS27764.1"
                     /translation="MSNHALFITQVHAARLQQPDAPLNARLLRECLQLRHDDAAGRRW
                     SARNYPGGYTSYDTASRMHERSPTFAALARRIDSHVRDFARALDYDIDVRHLAMTDCW
                     VNIMGRGAAHTGHLHPLSVISGTYYVRTPKGSAELKFEDPRLARFMAAPTRRRSARLG
                     NRSWVALPARAGHVVLFESWLRHEVSAQRVAGERVSISFNYALA"
     gene            13033..13284
                     /locus_tag="PKN91_06540"
     CDS             13033..13284
                     /locus_tag="PKN91_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27765.1"
                     /translation="MESNHMRQLDGQRRSAGRPNLKASVALDEFMSDLESLQPRDASA
                     RRSLDEARTAWQAREEIRAGHARTLTVNEVEHELDLGPA"
     gene            13281..13538
                     /locus_tag="PKN91_06545"
     CDS             13281..13538
                     /locus_tag="PKN91_06545"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNS27766.1"
                     /translation="MSAWQMRYHENAVDDLRRLGRHEARDVLAAMTERVTRQTSPHET
                     GELLISDEYGAYWRYPLPDCNVLCDIDRDARCVTVLHVAAR"
     gene            complement(13577..14572)
                     /locus_tag="PKN91_06550"
     CDS             complement(13577..14572)
                     /locus_tag="PKN91_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNS27767.1"
                     /translation="MSTSDRRMSRREAIAAGVATAIAATHSGAVLAAAGAGKTAVAGT
                     AAAINTRAIPATGERLPVVGLGTNNYSPVTAEERAARRAVIEQLPKLGGSVIDTAPAY
                     RQSETVLGELMAEIGNRDQIFLASKVTAPDGDRVRGVMMLEESFRRLRTARIDLMQVH
                     NLSGVDVMMPVLADWKEAGRIRYVGITTSSAPQYPEMLAAMRTHALDFVQVDYGIANR
                     GAADEILPLAKERGQAVLINMPFGGRRDGNIFRHVAGKALPDWAREIEAASWAQFFLK
                     YVVSHPAVTCAITGMTKVAHLEDNLGALRGPLPDASYRKRMEALWDNEIMAGETA"
     gene            complement(14559..14777)
                     /locus_tag="PKN91_06555"
     CDS             complement(14559..14777)
                     /locus_tag="PKN91_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27768.1"
                     /translation="MLLVHHLEYSRSQPVHWLLQELGLPRQARCHARDPATLALERWT
                     NPADVLNRIHGMLAYCRALKRGGHHVYV"
     gene            14954..>15192
                     /locus_tag="PKN91_06560"
     CDS             14954..>15192
                     /locus_tag="PKN91_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNS27769.1"
                     /translation="MSFAELGLTPGLLRAVADKGYDTPTAIQTRAIPAMLSGRDVLAS
                     AQTGTGKTAGFVLPLLQKLDAPHGRAPRALVLTPTR"
BASE COUNT         2558 a         5289 c         5169 g         2176 t
ORIGIN      
        1 tacgacagca gcgcgagccc gatgatcacc tggaaggtgc gtggccgcag caccagccag
       61 actccggaac ccgacaacac gccgattgcg agcgcgaaca ggatctccac ggctcagccc
      121 tccgccggtc gtggctcgac cggttcgcgc gggctgcgga aggactggtg cgcgagcgag
      181 acgaggatca gcatcgtcga gcccacgacc acgagataca cgccgaggtc gaacaacagc
      241 acggtcgcaa ggtggacatc gcccaacaac ggcaggtgca ggtcatacgc gagactcttc
      301 aggaacggtc ggccgaatgc ccacgcgagc atgccggcgc cacccgctga cacgagaccg
      361 agcgccaccc agtactgcgg gtgcacgcgc atgcgccact cgacccacat cgcgccgcca
      421 accatgtact gggtgatgaa cgcggtcgcc atcacgaggc cgccgacgaa gccgccgccg
      481 ggcgcattgt gcccgcgcag cagcaggaag atcgccacga gtcccgccat cggcagcagc
      541 agccgcacca gcacggcgga aatgcgcatg tcgccagagg ggagcggcgc gagcggatcg
      601 aaggagcgat agccgccgtg cgcgatttcc tcgcgctgcg cccggggcac ggtgagactc
      661 tccggcgccg gccggaaccg gcgcagcagc gcgtacacgg tgagcgcgac gatgctgacg
      721 acggtgatct cgccgaaggt atcgaagccg cggaagtcga ccaggatcac gttgacgatg
      781 ttgcgtccgc cgccttcggt gagcgcgcgc tcgacgaaga agcgtgccag gccatcgacg
      841 cgggggcggg tgagcacggc ataggagatc gcggccagcc cgccgccgac cacgatcgca
      901 acgagcaggt cgcggccgcg ccgcgcgcgc gcgcgcagcg tctggcgcct ggcatcgtcg
      961 aacatcagtc ggcgcggcag ccagcgcagg ccgagcagca gcagaaccag cgtcaccacc
     1021 tcgacggcca cctgtgtcag ggccaggtca ggcgccgaga accacgcgaa ggtcaggcag
     1081 gtcacgagac ccgcgccgcc catcatgatc agcgcggcga gccggtggaa cttggcctgc
     1141 acggcggcgc cgatggcgca gctgcatccg accacccaca ggagcgcata cacggggtcg
     1201 atcggcgtga gcgcctcctg tccgcgggtc cagcccgtcg cagcgatcgg aatccagccg
     1261 acggccagcg ccacgcagat gatcatcagc agctgcggct gcaggcggcg tgacgaggcg
     1321 aagcgcacca tgcgttcggc ggaccggtgg atgacgatga tcaggatatc gaacgcgcgc
     1381 cggccatcga gcggcgggcg caccggggcg cggccgacgg cgcggtcgcc gtagaaccag
     1441 cgcacgagat agatgccgag gccgcccagc atcgcgatca cgctcatcag cagcggcagc
     1501 gtcagcccat gccagacggc gaggctgtac tcgggcgtgt cgataccgag tatcgaagtc
     1561 accgcgatat cgaggagcgg accgatcgtg cgcatcggca ggatgccgac cagcagacag
     1621 gcgagcacga gcagcatgct cggcagcagc atgccgcgcg tcggctcgtg cggcgtgcgc
     1681 ggcagatcct gcgccggcgg accgaagaac acctggtgga tcaggcgcag cgagtatgcg
     1741 acgctgaaca tgcccgccac ggtggcgatt gccggcagtc cgtagcgcag taccgggcca
     1801 ccgcccgcga agatcgtctc ggcgaagaac atctccttcg acaggaagcc gttcagcagc
     1861 ggcacgccgg ccatggccgc agcggcgacc gtggcgacag tcgcggtgat cggcatcgcg
     1921 agcgccagcc ctccgaggcg cttcaggtcg cgcgttccgg tttcatggtc gacgatgccg
     1981 gccgccatga acagcgaggc cttgaaggtc gcgtggttca tcatgtggaa tacggcggca
     2041 acgagtgcca ggcggctgtt catgcccagc agcagcgtga cgagcccgag gtggctgatc
     2101 gtcgagtagg cgagcacgcc tttcatgtcg tgctggaaca gcgccgccgc cgcaccgagc
     2161 aggagcgacg cgagtccagc cccgcacacc agcgcgaacc acaggtcggt gccggcgagc
     2221 accggccaga gccgcgcgag caggaaaacc ccggccttga ccatggtcgc cgagtgcaga
     2281 taggcggaaa ccggggtagg cgccgccatg gcatgcggca gccagaaatg gaacgggaac
     2341 tgcgcgctct tggtcagcgc accgagcagg atcagcacga gcatcgccgg ataccacggg
     2401 tgcgccgcca cgcggctgcc ggcggccagc acgtgatcga ggtcataccc gccggcgatt
     2461 tccccgagca tgatcacgcc cgccagcagg cagaagccgc cggtggcggt gaccgtgagt
     2521 gccatgcgcg caccctggcg cgcatcgacg cggtgatacc agtaggcgat cagcatgaac
     2581 gagctgaagc tcgtcagctc ccagaacacg accagctgga tcaggttgcc cgacagcacg
     2641 atgccgagca tcgaacccat gaacgcgagc aggaacgaga agaagcgcgg caccgggtct
     2701 tccggtgaca tgtagtagcg cgcgtagagc acgaccagcg cgcccatgag ggacacgagc
     2761 agcgtgaaca tccacgcgaa gccgtccatg cgcaggtaga actcgaggcc cgcctgcgcg
     2821 agccacggca actgcaggcg aaccacaccg ccctcggcga tgtccggata cagactgatg
     2881 gtgagcccgg tgcacacgag cgcgaccgcc ccggctagcc aggcttcggc attgcgggcg
     2941 ttggaaggca gaaatgctgc gcacaagctg ccgacgaacg gcaacagcac gatcagcgca
     3001 agcgacatgg atctcccgac aacacgcgcg caatgtctct aactgcgagg ataagtcctt
     3061 atttttacca caggttttgc gcctctgcgc ggcgggttta tcagcccgcg cgtgagcagc
     3121 tggcccccga gcgcgcagaa atagagatag cccctcagcg tcccgaagcg ctcgatcgtc
     3181 gcgtcgatct tcgccgtgct cgtcgtgccg agcagtgcgc gcaggctgcg cgcggcgccg
     3241 acatcgcccg gcgggaagac atcgagtcga ccgaggccgc gcagcaggac gaggcccgcg
     3301 gtccacgggc ccaccccgcg cagcgcgacg agccgcgctc ccgccgcctc gctcgacagc
     3361 ctgtcgagtt cgcgctcgtc gagtgcgccc gaatcgatcg cgcgcgcgat catctggagt
     3421 gtgcgcgtct tctgcgcgct cagaccgcag gcgcgcagcg cgcgcggcct ggcgcggacg
     3481 atcgccgcgg gtgtcggaaa cgcgtagtag cgctcgccct cgtggacgag cgcgtcgccg
     3541 aagcgctcga cggtgcgccc gagcaccgcg atgccggcgt cgatactgac ctgctggtag
     3601 ggcacgacgc tcgcgaaggc ctcgaacagc ccgccgaacc ttggtgggcg caggccgcac
     3661 agtgcgcgcg ccgtcgcccg catggccggt tcgcgctccg ccgcgcgccg cagtaccacc
     3721 ggatcgagat cgaggcccag cacccgccgc agcgcccggg ctgccgtgtg ccgcgcgctc
     3781 gccgagtgcg ggccgtgcag cgtgcagcgc aggtccggcg ccgcgacagt gccgcgatcg
     3841 acgacctcgg cgagcaacgg gccctcgccg gtgtcgatga cgcgtcgata gcacccgtcc
     3901 gaccagacat cgacccgatt ggcgggccgc cgctgcagca cgcgcacggt tgcttcgagg
     3961 tgaaacggcc cgcgggtcgg cagccggatc gccaccgcgg gctcagcgct gttcgcgcag
     4021 gaaatcgcgc agcacgagcg cgctgttgtg ctcgaggtcg cgggcgctgt acaggagtgt
     4081 gaccggcccc cggcggctcg ccgcgagcag cggctcgcat gccgcgcgat tcgcagccag
     4141 ctccttgcgg tagcgctcac gaaactcgtc ccagcgctcg acccggtgcc cgtaccagct
     4201 gcgcagcgcc gcactcggtg ccacgtcctt cagccagggc acgccgtcga gcgcttcctt
     4261 gcgcacgccg cggggccaca ggcgctcgac caggaagcgc tcgccatcgc cgcgctcgcg
     4321 cgcgtcgtag acccgcttga tcctgatcac cccgcctcgt ccccgatcaa ctgcccgagc
     4381 acatagggca ggatgccgcc cgcctcgtag taggccactt cgatcggcgt gtcgatgcgc
     4441 acggtgagcg gcacgctcac acgctcgccg cccacgcgca gcacgtgcag tgtcgccgcc
     4501 acgcgcggcc gcagcctatt cgcgagctcg atctcgaata cctcgctgcc gtcgagaccg
     4561 agcgattgcg cagtggtgcc ttccggcaac tggcacggca gcacgcccat gccgatcagg
     4621 ttcgagcgat ggatgcgctc gaagctgcgc gcgatcaccg cgcgcacacc gagcaggcgt
     4681 gcccccttgg cggcccagtc gcgggagctg ccggcgccgt agtcactgcc ggcgatgacg
     4741 agcagcggca cgccctcggc acgataacgc tcggcggcat cgtagatgct gagcacctca
     4801 ccgccgggct ggtggcgcgt ctcgccgccc tcggtcccgg gcaccatctg gttgcggatg
     4861 cggatgttcg cgaaggtacc gcgcaccatg acgtcgtggc tgccgcgccg cgccccgtag
     4921 ctgttgagat cctcgatggc gactccgcgc gcctgcaggt attcgccggc gggcgaaccc
     4981 ggcttgatcg ccccggccgg gctgatgtga tcggtggtga tcgaatcgcc gaagatcgcg
     5041 agcgcccgcg cgcccttgat cgtgccggcc gggtaggccg ccatcgacgc atggaaatac
     5101 ggcggctcct ggatataggt cgaggccgga tcccagtcgt acagctggcc gctgccggac
     5161 gggatcgcgt tccacagcgg attgcggcgc gcgaagtcgc cgtagatgcg gcggtacact
     5221 gccggatcgg cggcgtggcc acgcagcgcc tcgatctcct cgagagtcgg ccacaactcg
     5281 cgcaggtaaa taccgcgccc gtccacttcc gcaagcggtt cgcgcgtcag gtcgatgtcg
     5341 acgcggccgg cgatcgcgaa cgcgacgacc agcggcgggc tcatcaggaa attggccttg
     5401 acgctctggt ggatgcgcgc ctcgaaattt cggttccccg acaacacgct cgcggcaatg
     5461 atgtcgtggc gcgcgatcgt ctcctcgatc tcgcgatcga gcggccccga gttgccgata
     5521 caggtcgtgc agccgaagcc gacgatgttg aagccgaggc gatcgaggta ggcctgaagc
     5581 ccggtctgcg cgaggtattc gctgacgacg cgcgagcccg gcgcgagcga ggtctttacc
     5641 cagggaggca ccgtgaggcc gagctcgacc gccttcttcg cgagcagccc ggccgcgagc
     5701 atcacggccg gattcgaggt gttggtgcac gacgtgatcg ccgcgatcac gacgtcgccg
     5761 tgcgcgagct gcgcggctac ggcgccctcc ccgcggccgc gcccgtcgcg acccggcgcc
     5821 ggtacgcgat tgggtgtcgg gcgattgtcc atcatctcga gttcggtgcg catgttggtg
     5881 ttgcgcgggc gcgggtcgga aatgccgccg gtctgccggc cgccgccggg gcgcggttcg
     5941 ccgccctcgc ccagcacctc gaagtgttca ccgacttccg tctgcggctt gccgtagccg
     6001 ttcgcaccgg tcagtagcga caggaactcg gtcttcagcc cgccgagcac gatccggtcc
     6061 tgcggacgct tcggtcccgc gaccgccggc tcgacggtcg cgaggtcgag atcgatcagc
     6121 gtcgtgtagt cgcactcgcc cgcgagcggc atgccgaaca tgccctgcgc gcggtaatag
     6181 cgccgcacag tctcgacctg ctcggcgctg cgcccggtgg cgagcaggta gcggcaactc
     6241 tcctcatcga ccgggaagta gccgatcgtc gcgccgtact cgggcgacat gttgccgatc
     6301 gtcgcgcgat cgggcaccgg caggctcgcc gcgccttcgc cgtggaactc gacgaacttg
     6361 ccgacgacat gcgcatcacg cagcagctgc gtgatgcgca gcaccaggtc ggtcgcggtc
     6421 gctcccgagg gcaagcggcc gtgcagatgc acgccgacca catcgggcag cagcaggtaa
     6481 acgggctggc cgagcatcgc ggcctcggcc tcgatgccgc cgacgcccca gccgacgatt
     6541 ccgaggccgt tgatcatcgg cgtgtgcgag tcggtaccga cgaggctgtc ggggtagagc
     6601 gtgtcgccgc gcgcgagcac gccctgggcg aggtactcga ggttgacctg gtggcagatg
     6661 ccgaaacccg gcggcacgat gcgcaggccg tcgaatgcct gcgcgcccca cttcaggaac
     6721 tggtagcgct cgtggttgcg ccgaaactcc atgtccatgt tgaactgcag cgcgtcgtcg
     6781 cggccgtagc ggtcgacctg caccgagtgg tcgatgacga gatccaccgg cacgagcggc
     6841 tgcacgcgcg tgatgtcgcg cccgcgccgc tgcacggcgg cacgcatcgc ggcgagatcg
     6901 accagcagag gcacgccagt gaaatcctgc agcagcacgc gcccgacgac gaacggcact
     6961 tcggcatcgc gcctggcctg cggttcccag cgcgcgaggg cttcgacgtc ctcgtcgcgg
     7021 atacgccgcc catcggcatt gcgcagcacc gactcgagca gcacgcgcag gctcgccggc
     7081 aggcgcgaaa tcctcccgac gccgagcttc tcgagctccg gcagggaatg gaaccagcgc
     7141 tggggtccgg cgggcagatc gagtggccgg cgtgtatcga gcgaggaacg gcgcgctttc
     7201 gccatggctc gagtataggc gcctgggccg gcgttttcgg cccctgagcg tcagcgctgc
     7261 gctgccctcg gcggcggcac cgcgaggatg tcgcactcga tttcgcgcag cacacgatcg
     7321 gccacgctgc cgaggaaaac ccgctttagg ccggtcaggc cgcgtgtacc gaccaccagc
     7381 agctgcggct cctgttgcag cactgcctgg tggatcgcgt cgaacggcga gccggagtcg
     7441 acagtcagct cgacgcggcg gtcagcgaga tcgcctgtgg cgaggaaggc gcgcagcctg
     7501 gcgcgcgctt cccgttccca ggcatcgcga taggcgcgca cctcctcggc cgaggcgccg
     7561 ctgccctcga gcgcgccggt gaaaaccgcg tcgaaggcat gcacgaccga aatgtcgcca
     7621 tcgagcaggc cgagttcacg ggccgcctgc accgcgcgcg ctgacgccgg cgagacatcg
     7681 agcgcgagca gcacatcgcg gtaaggccca tgctcgtcgg tattcacgac cagcaccggc
     7741 acgccatcgg cgcgcagcac gcgctcgagc gtcgagccga tgaacacgtc ggcgagcgcg
     7801 cgccgacggt gcgcgcccat gacgatcagt tccgcgttcc actcgcgtgc gtagcgcgcg
     7861 atcgactcga atgcattgcc ggcgaccacc tcgatcgccg cgatcgcgcc gtgcccgccc
     7921 ggcagcgagg ccgcctgcgc ctcgagatgc gcccgcgcct cggcctgcct cgcctcgatg
     7981 atgtggtggg gctggtcatc gtcgaccacg tgcagcagca gcaagcctgc accatgctgc
     8041 tgcgcgaggt tcgcggcacg ccgcatcgcg cggtcggacc gcttcgacag atcagtggcg
     8101 cagagcagtt ttttcattgc agtgcccgtt ccacggccgg gtgcacgatg cgcccggcga
     8161 cgacattgag cccggcgtgc agacccgggt cacgcgcagt ggcttcacgc cagccgagcg
     8221 tggcgagccg ctccacatag ggcagcgtga cattgttcag cgcgaaggtc gaggtgcgcg
     8281 gcactgcacc cggcatattg gtcacgcaat aatgggtcac gccctcctcc gtgtaggtcg
     8341 ggtcggcgtg cgaggtgggc cggctcgtct cgaggcaacc gccctggtcg atcgcgatgt
     8401 cgacggcgac ggcgcccgca cccatcgcgc gcagcatggc gcgagtgacg acgcgcggcg
     8461 cggcggcccc cgggatcagc acggcgccga tgagcaggtc ggcgcgtgca agcgccgcca
     8521 gaatcgactc cggcgtcgaa aacaggatgc gcgcgcgacc ttcgaagcgc gtgtcgagtt
     8581 cgcgcaggcg cggcaccgaa cgctccagga tcgtcacgtc ggcgcgcatg ccgatggcga
     8641 cctccgccgc gtggctgccg gacacgccgc caccgatgat cacgacgcgc gcgggcgcca
     8701 cgcccggcac gccgccgagc agcacaccgc gcccgcccag cgccttggtg cagtactggg
     8761 cgccgacctg cacggacatg cgcccggcca cttcgctcat cggcgtgagc agcggcagcg
     8821 agccatccgc tccgacgacg gtttcgtagg cgatggcggt gcagccggag gccatgagcg
     8881 cttcggcctg ggcacgatcg gccgcgagat gcaggtaggt gaagagaacg tgctttggcg
     8941 tgagccggcg gcactcctcg agctgcggct ccttgacctt gacgatcagt tccgcctcgc
     9001 cgaaggtttc cgcggccgag ctggcgatgc ccgcgccggc ggcggcgtaa tcggagtcgc
     9061 tgatgccgat cccggcgccc gcgccggttt ccaccgtaac cgtgtgcccg gcctccgtga
     9121 gtcgcctcac ggcatcgggc acgagcccga cccggtactc gtggttcttg atttctctgg
     9181 gtacgccgat tctcatggct cgcttccctg tgtatcgccg gcaggcattt tgcagccgcc
     9241 ggcacgacag acgttgcggt ctgtgcgaca ccttggccgg gtttgcgcaa taatcttgcg
     9301 catggacagc acggatcggc gacttctggc ggccttgcag cgcgacgggc gcgccacgaa
     9361 cgcggcactg gcggcccagg tgggcctgtc ggaatcggcg tgcctgcgac gcatgcgtcg
     9421 cctggaagag accggcgtga tccgcggcta cggggcgcgc atcgacgccg gtgccgtcgg
     9481 ttacacggtg cgcgtgctcg tgcgcatcac gctcaagagc cagacggatc gcgacttcaa
     9541 cgccttcgag cgtgccgtgc aggccgtgcc caatgtcatc gactgctttc tcaccaccgg
     9601 ccagtgggac tacgaactca tcgcgatcgc gcgcgatgca gccgatctcg agcggctgca
     9661 cagcacggtg cttacgaaac tacccggggt ggcgcgtgtg gaatcgagtt ttgttctgcg
     9721 ccacgtggtg cgccaggtgt cgctgcccct cgatcgctga ccggctcgcg cgtgacctgc
     9781 aggatgagca ggcgccgcat cccgccgggc attcgccact ccacctcgtc gccttcgcgg
     9841 tacccgagca gcgcggtgcc gagcggcgcc agcaccgaga tccggcgcgc ggcgatatcg
     9901 gcctccgacg gcagcaccag cgtgtatgcg tcttcgcggc ccgtgacgac gtcgcgcacg
     9961 cgcacgcgtg cgttcatggt gatcacgtcg gcggggactt catccgcgag gcgcacgacc
    10021 gcacgctcca gctcggcctt gagctggtgc aggtgctcct ggtcgtgggc cgaatcgtcc
    10081 tgtgcgccaa gtaacgcgcg cagccgatcg gcatccgcct cgctaacaag gatcgggttc
    10141 tgtctcacaa tctgctccaa aggaatgcct gcctgtttac ctgacagtgt cagttagatc
    10201 aattagaatt aacaattaag atatttcgat tttttaacac catgacaggc aagccgaagc
    10261 gcctcaacta tcagcatctg tactatttct ggaccgtggt gcgctgcggc agcctcgcgc
    10321 aggcgagcgc cgagctgaac ctgtcgatgc cgacgatcag cgcgcagctg cgcacgctgg
    10381 aggaacggct cggcgtcgag ctgctggtca aggccgggcg gcgcctcgtg ccgaccgacg
    10441 cgggccgcac cgccttccgt tatgccgagg acatcttccg gctcggccgc gagctcaccg
    10501 atgcgctcac gccgggtggc acggcgcagc cgctgcggct cgcggtgggc atcgacgacg
    10561 tgctgccgaa ggagatcgcc gagcgcctcc tcgcgccggc cctcggccag ggccgcgcat
    10621 tgcggctcat ctgccaggaa ggcaccctcg agcggctgac cgctgcgctc gcggtgaacg
    10681 acctcgacct ggcgctctcc gatgcgccgg tgccgccgcg cctcggcatc cgcgcgcaca
    10741 gccaccgact cggcagctgc ggcgtgtcgt ggatgggcgc gcgggcactc gccggcaagt
    10801 tgcgcgcgca cttcccggcc tcactcgacg gcgcacccgt gctgctgccg accgacgata
    10861 cggcgatccg ccgcgcactc gaccagtggt tcgagcgcca caagctgcgc ccgatcgtcg
    10921 ccggcgaatt cgaggactat gcgctgctgc gcgagttcgc acgcgcgggc acgggtctga
    10981 tcccggtgcc ggacgtgatg gtgcggctgt atcagcgcga ggcgggcctc gcgctgctcg
    11041 ggcccgcccg gtccgtggaa gccgagttct atggcgtgac cctgcaccgc gacatcagcc
    11101 accccgcggt ggcggagatc tgcgcacagg cccccctgct cttcagccgg ggcgctggtc
    11161 cacgcgtggt ttcgtgagaa tgggcgcgta gacgaacaca tagatgccga aggcggcaat
    11221 ccacaagagc gccgccgcct cgatcgtcag caggtaatcg ggcatgactg ccgggccgaa
    11281 cacgcgcacc agcgcggcga gcagcagcag catgtaggcc acggcgatgc tgcgcgcgac
    11341 gacgagcggg cgacccgtgt ggccgagcga ggcgcgggtg gtgaccgcga ggatcatcgt
    11401 cgccgcgaca ccgatggtca gcgcgtgctg ccaatgcgcc gcccaggccg cgccggtcgc
    11461 aatgtggacg gctttcagtg cgagcccgat cggcagccag gcgtaggccg catgcagcac
    11521 ccacacgatc gggtcggaca aggtgcgcag gctgcgccaa cccgacatgc gcaccgcctg
    11581 cgcgagcgcg gcgacgcccg caaccgccca ggccgcccag tgcgacggcg ctacgaggtc
    11641 gaccacgacc atgagcacca tcgagccgat cgtgatgcgc tctacgggcg gacggctgcg
    11701 gatcgccgcc tcgatgccgc gctggcgcag cgcgcttgcg gtgaaattcg gcacgatgcg
    11761 cccgccgatc accgtgacca gcagcagcac gaggccgatg ccgacgcgca gcgcgcgact
    11821 cgcgagcgcg aactcgccgt tcgtgatcga gtagaggaac caggcgtcga tcgcccacag
    11881 caccgcgatg atgatcagca tcgggaaatt gcggttgtgc gtgcgcagca gcgtgcggcc
    11941 gatcaggaat gcgagcagcg gcaggaacga cagctcgcag accgcgagcc acagcggcgg
    12001 cagcgaaccg gtggccgcga acgcaatccg acccgcgagc cagagtacgg tgagcgcgac
    12061 cagcggcgga ccggcgaagc cgcgcgtgcc ggtccagctc ggcaccgcgg tcagcaggaa
    12121 gcccgcgatc gccgcaccga tgaagccgaa cagcatttcg tggccatgcc acagcaaggg
    12181 cggcagcgca gccgacggca ccctgccggt cgcgtagatc cacacccagc cggcgatcgc
    12241 gatgacggca tagaggcccg cgaccaggaa aaaagggcga aaaccatagg cgaagggcac
    12301 gaaacgcgaa tcagtgcgca tcccggcatc atagtcgaat gcaccggcga cccgcattgc
    12361 gccagcccaa tgcgtatcag gccagcgcgt aattgaaact gatgctgacg cgctcgcccg
    12421 cgacgcgctg cgcggacact tcgtggcgca gccagctctc gaaaagcacg acgtgccccg
    12481 cccgcgcggg caacgcgacc catgagcggt tgccgaggcg cgcactgcgc cgccgcgtcg
    12541 gcgcggccat gaagcgcgcg aggcgcggat cctcgaattt cagttccgcg gatcccttcg
    12601 gcgtgcgcac gtagtaggtg ccgctgatga cggacaaggg atgcaggtgg ccggtgtgtg
    12661 cggcgccgcg ccccatgatg ttgacccagc agtcggtcat cgcaaggtga cgcacatcga
    12721 tgtcgtaatc gagcgcacgc gcaaaatcgc gcacgtggct gtcgatgcgc cgcgcgagcg
    12781 cggcgaaagt gggcgaacgc tcgtgcatcc ggctggcggt gtcgtaggag gtgtagccgc
    12841 ccggataatt ccgcgcggac caccgcctgc ccgcggcatc gtcgtggcga agctgcaggc
    12901 attcccgcaa caaacgcgca ttgagcggcg catcgggctg ttgtagccgc gccgcatgaa
    12961 cctgggtgat gaacagcgcg tggttgctca tggcattcgg cgtattttgc actacactgg
    13021 cccgaggcca ccatggagag caatcacatg cgtcaactcg atggtcagag acgatccgcc
    13081 ggccgtccca atctcaaggc cagcgtcgcg ctggatgaat tcatgagcga cctcgaatca
    13141 ctgcaaccgc gcgacgcgtc cgcgcgccgc tcgctcgacg aggcgcgcac tgcctggcag
    13201 gcccgcgagg aaatccgcgc cgggcatgcc cgcaccctca ccgtcaacga agtcgaacac
    13261 gagctcgacc tgggtcccgc atgagcgcgt ggcagatgcg ctaccacgag aacgccgtgg
    13321 atgacttgcg gcggctcggg cggcacgaag cacgcgacgt gctggcagcg atgaccgaac
    13381 gggtcacgcg acaaacgagt ccgcacgaaa ccggcgagct gctgatcagc gacgaatatg
    13441 gcgcatattg gcgctatccg ctcccggact gcaacgtact gtgcgacatc gaccgcgacg
    13501 cccgctgcgt cacggtgctg cacgtcgccg cgcgctagct ggcgcagcgc cggccgctgg
    13561 ccggcgctgc cgccgctcac gcggtttcgc ccgccatgat ctcgttgtcc cacagcgctt
    13621 ccatgcgctt gcggtagctc gcatcgggca atgggccgcg cagcgcgccg aggttgtcct
    13681 cgagatgcgc aaccttggtc atgcccgtga tcgcacaggt caccgccggg tgcgacacga
    13741 cgtacttcag gaagaattgc gcccaacttg cagcttcgat ctcgcgcgcc cagtccggca
    13801 gcgccttgcc ggccacatgc ctgaagatat tgccgtcgcg gcgcccgccg aatggcatgt
    13861 tgatgagcac cgcctggccc cgctctttcg ccaggggcag gatttcgtcg gccgccccgc
    13921 gattggcgat gccgtagtcg acctgaacga aatcgagcgc gtgtgtgcgc atcgcggcca
    13981 gcatctcggg atactgcggc gcgctcgagg tggtgatgcc gacgtagcgg atccggcctg
    14041 cctctttcca gtcggccagt accggcatca tcacatcgac accgctgagg ttgtggacct
    14101 gcatcaggtc gatgcgtgca gtgcgcaggc ggcggaagga ctcctccagc atcatgacgc
    14161 ccctgacgcg atcgccgtcg ggagcggtga ccttgctggc caggaagatc tggtcgcgat
    14221 tgccgatctc cgccatcagc tcaccgagca ccgtttccga ttgccggtac gccggcgccg
    14281 tgtcgatcac gctgccgccg agcttcggca gctgctcgat caccgcccga cgcgcggcgc
    14341 gctcctcggc cgtcaccggg ctgtagttgt tcgtgccgag gcccacgacc ggcagccgct
    14401 cgccggtggc ggggattgcg cgcgtgttga tggccgccgc cgtgccagcc actgccgtct
    14461 tgcccgcgcc cgccgccgcc agtacggcgc cggagtgcgt ggctgcgatg gcggtcgcga
    14521 cacctgctgc gatagcttcg cggcgtgaca tgcgtcgatc agacgtagac atggtgacct
    14581 cctcgtttga gcgcgcggca atacgcgagc atgccatgga tgcggttcag gacatcggcg
    14641 ggattcgtcc agcgctcgag cgccagggtc gcgggatcgc gcgcatgaca tcgcgcttga
    14701 cgcggcaggc ccagctcctg gagcaaccag tgcaccggct gcgagcgaga atattcgagg
    14761 tggtggacga gcagcaccgg gcgattgtgc acaacgggcc gcgaagagtg ccacccatac
    14821 gcaacgcgaa gtgaccccgg cggggctttc gtgtaagctg caccttgttg aaaacgctag
    14881 agccgcgcca tccgcgcgct cgtggggccc gcgccgaggc ccggccctac tccactatct
    14941 ccaaggatca tccatgtctt ttgctgaact cgggttaacc ccgggactcc tgcgtgccgt
    15001 cgccgacaag ggctacgaca cccccaccgc catccagacc cgcgccatcc ccgcgatgct
    15061 ttccggccgc gacgtactcg ccagtgccca gaccggcacg ggcaagaccg cgggattcgt
    15121 cctgccgctg ctgcagaaac tcgacgcgcc ccatggccgc gccccgcgcg cgctcgtgct
    15181 gacccccacc cg
//
LOCUS       DAOQVY010000052        14723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_4611_length_14723_cov_6.020385, whole genome shotgun sequence.
ACCESSION   DAOQVY010000052 DAOQVY010000000
VERSION     DAOQVY010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14723
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4611_length_14723_cov_6.020385"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PKN91_06565"
     CDS             complement(<1..988)
                     /locus_tag="PKN91_06565"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HNS27770.1"
                     /translation="MRFEGVASAGGLALALVLAAPWAEARAPARIVYLEPARAMQAQR
                     DAASGHERITFEAYGKRFELQLEPNARLRGAVPPERGDLVALEGRIVGLPGSWARITR
                     TRQGLNGLIADGRELYAVEPAQQVAAFADAALTPPTSGTVVYRLEDALIDEGAVSCGV
                     AAAGEAANALALYEAVGAELTALATVVVDKQLNVAMIADYELYQDYGSSTVDFLVARM
                     NIVDGIYASQAGLRVRVGSTRIHDTPQDPYTASNAEELLRQVAETRRLAPVSQRLALT
                     HLMTGRELDDTTVGIAYLDAICSLNSASLTEARAGKLSPAVISLIAAHEKLAV"
     gene            complement(1049..2266)
                     /locus_tag="PKN91_06570"
     CDS             complement(1049..2266)
                     /locus_tag="PKN91_06570"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS27771.1"
                     /translation="MPKHIVIVGAGQCAAQAVETLRRRGYTESLTVIGDEPLPPYQRP
                     PLSKKYLTGSLEPERLLIRQPSFYEEHGVVMRLGRRAVRIDRAARQVQLDDGATLAYD
                     TLLLATGSRARRLAVPGADLPGVHTLRSLADVDAIRAAAQPGRQAVIVGGGYIGLEAA
                     ATLRELGLEVTVIELADRVMNRVVCEQVSRFYEAEHARHGVQILLRSGLDAFVGDTRL
                     RAVRDAGGTAHPADLAIVGVGVLPVDELAREAGIACDNGILVDEYCRSNDPHIFAAGD
                     CTSHPSLRYDRGVRLESVDNAFEQGTSAALNMLGIATVHDKTPWFWSDQYDLKMVIVG
                     LSQGHDRIVLRGDPATRSFSACYLREGELIAIDTVNSPRDQMAARKLIAARARPDPAR
                     LADIMLPLKDCAG"
     gene            2473..2811
                     /locus_tag="PKN91_06575"
     CDS             2473..2811
                     /locus_tag="PKN91_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNS27772.1"
                     /translation="MKLVTAIIKPFKLDDVRAALAEIGVSGMTVTEVKGFGRQRGHTE
                     LYRGAEYVVDFVPKTRIEVAVADKLVDQVVDAVLKAAKTGKVGDGKIFITGIDRVVRI
                     RTGETDDAAL"
     gene            2814..3188
                     /locus_tag="PKN91_06580"
     CDS             2814..3188
                     /locus_tag="PKN91_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27773.1"
                     /translation="MSAGATGRQALQRAVAALEIGSARRHIFLCVGGKCADEDEQQAS
                     WKFLKRRLRELGLVDRPQGVLRTKADCLRVCVGGPIAVVYPEGIWYRRCTADNLERII
                     QEHLIGGRIVEDLRIAQTGSPA"
     gene            3198..4172
                     /locus_tag="PKN91_06585"
     CDS             3198..4172
                     /locus_tag="PKN91_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HNS27774.1"
                     /translation="MPRAIVIRETGGPEVLRVEEVAVAAPAAGEVQLRHTAIGLNFID
                     VYDRSGLYALALPAVPGREAAGVVTALGPKVKGLRVGDRVAYALSAPGAYAEVRNVPA
                     ARVVKLPKGVKDEVAAAIMLKGLTAQFLLRRTWRVRKGEWILVHAAAGGVGLLLCQWA
                     RALGAKVIGVVGSEAKAKLAKSHGCHHVLLAGRDDIVERVKALTKGAGVHVVYDSVGK
                     DTFFTSLDCLRPRGLMVSFGNASGPVPAIAPLELAKRGSLYLTRPTLFSYIATSRELA
                     AAARELFAMVTKKKLRVRIGQRYALADAAQAHRDLEARRTTGSTVLVP"
     gene            4169..5413
                     /locus_tag="PKN91_06590"
     CDS             4169..5413
                     /locus_tag="PKN91_06590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS27775.1"
                     /translation="MTRAGAAWAVLALTGIALFGNYYVYDSIGPLAEQLARELGYSDT
                     QIGTLNAIYSVPNIVLVFVGGVLVDRYGPGLVTFWTALVCLAGALLTAVGTPFAVMAA
                     GRLLFGIGAETMIVATIVALGIWFAGRQLALAMALSISFARLGSYTVDLSPLWAPDAY
                     ASGWQAPLWLAAGVMFAGCLAAWVYRRLEANAAARGVQVQGLSRERIELRSLAHFNRG
                     YWYVLALCVLFYAVIFPFRSTFAIKYFQHTGGMTLEAASVLNSHVFLAAVIATPLFGW
                     VNDRIGRHGLLLTLGAASLVAAFCVLGATDLSPGVSTALIGVAFSLVPAVLWPAVTLL
                     VAPQRLGTAYGFMTMLQNIGMAGANFTAGALNDANGASASHPDGYLPMIGFFAALSAL
                     ALVAAVLFWRYLSHHGAREPAS"
     gene            5480..5917
                     /locus_tag="PKN91_06595"
     CDS             5480..5917
                     /locus_tag="PKN91_06595"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNS27776.1"
                     /translation="MSNPPLRTDYPVFRALATRWMDNDHYGHINNVAYYSFFDTAVNG
                     WLMDATGVDIRTLPAIGLVVETGCRYFKPLSFPDALEVGLGIERLGTSSITYRLAIFR
                     AGEDSAAAAGRFVHVYVDAQTRQATAIPDVIRAAAQALREPIR"
     gene            complement(5897..7168)
                     /locus_tag="PKN91_06600"
     CDS             complement(5897..7168)
                     /locus_tag="PKN91_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS27777.1"
                     /translation="MSARLPPQKRVVVIGGGIAGLACAWYAAQDGHVVTLLDRGGPDT
                     DRCSLGNAGMIVPSHFIPLASPGAVLRGLRSLADPRSPLRVTPRADPDYLRWCWQFLC
                     AARASRVALAAPALRDLALLGRRGYEALTLLWPQGVPLAEHGLLMLARSAHGMREERR
                     VAAMAQRLGMPVEILDRDAAAALEPGVDMAIVGAVRYPLDAHTVPQRVVATLVAGLAD
                     AGVDLHWNTEVTGLERKGARVSGAVTDAGVVGGDEFVIAGGAWSAALARDIDLPLPLL
                     SGKGYSFMLPAPRARLHACAVLTEDFVAVTPMGDALRFAGTLELCGLDRSIDQRRVAA
                     MVDAIPRYYPQFGPADFAGITPWAGLRPCTPDGLPFLGRTRRADNVLLATGHAMMGVT
                     LAPASGRIISDLIAGRDPSVPISLYSPDRFA"
     gene            complement(7165..8076)
                     /locus_tag="PKN91_06605"
     CDS             complement(7165..8076)
                     /locus_tag="PKN91_06605"
                     /inference="COORDINATES: protein motif:HMM:NF017365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HNS27778.1"
                     /translation="MRVVDSHTGGEPTRVVIDGGPALTGSFAARVEDLRRNHDHFRSA
                     IVNEPRGSDVLVGALLTPPLAPGCTAGVIFFNNVGYLGMCGHGTIGTIVTLAWLGRIA
                     QGEHRLETPVGVVTATLHDANTVSVRNVPAWRHARGVRVEVEGHGPVTGDIAWGGNWF
                     FLCEDHGRRLALADAAALGEFCGRIRRALARAGITGPGGVEIDHVELADEARNFVLCP
                     GGAYDRSPCGTGTSARLACLAADGRLAPGEVWRQTGITGSVFESRYERDGERIIPTIT
                     GQAWVTAEATLLMDAADPLAHGIRVAP"
     gene            complement(8082..9005)
                     /locus_tag="PKN91_06610"
     CDS             complement(8082..9005)
                     /locus_tag="PKN91_06610"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNS27779.1"
                     /translation="MTVPWRDVFPAMTTQFRRDESLDLEAGSRHIEAMVASGASGLVM
                     LGSLGENPTLAPAEKRAVVAAALEVAAGRVPVIATVFEASTAAAIAFARDMERLGVDG
                     LMLLPAMVYRADPRETLLHYRAVARATQLPIICYNNPLAYHVDITPAMFVDLAGVSNL
                     VAIKESSGDVRRITDLRNALGDRYVLFAGVDDLALESAVLGADGWIAGIGLAFPRENQ
                     RLWDLAVAGAVDAALRLYRWFTPLAHLDTHVKFVQYIKLAVQAAGLGAEWVRAPRRVL
                     VGEERRRILGIIEAGLEHRPKLPPLPRRRRR"
     gene            complement(9036..9536)
                     /gene="ilvN"
                     /locus_tag="PKN91_06615"
     CDS             complement(9036..9536)
                     /gene="ilvN"
                     /locus_tag="PKN91_06615"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNS27780.1"
                     /translation="MRHIIAILLQNEAGALTRVAGLFSTRGYNIESLSVAATDDPTVS
                     RITLVTRGSEAVMQQIANQLLKLIDVVAVEDLTDGEHIERELVLAKLRLRPEQTDVVR
                     GYVVRRGGRVLDPAPDGFVVELTASEAEVSDFIAELAHYTEAVEVVRSGALGVSRAAR
                     NLKLVG"
     gene            9681..11516
                     /gene="ilvB"
                     /locus_tag="PKN91_06620"
     CDS             9681..11516
                     /gene="ilvB"
                     /locus_tag="PKN91_06620"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNS27781.1"
                     /translation="MPRPQAHPLAGQAMTGAQIIVQVIADEGVSAIFGYSGGAILPTY
                     DAVFLYNEARQLDGGQQMPLIVPANEQGAGFMAAGYARSSGRVGVALVTSGPGATNTV
                     TPIRDSMADSVPMVVICGQVPTAAIGSDAFQEAPVSSIMGAVAKHVFLVTEPEKLEAT
                     VRTAFEVARTGRPGPVVIDVPKDVQNWQGQFHGQGTLPIPGYRRRMDSAKRSAMADAD
                     AARFFELLAASERPLIYAGGGVVNGSASEALREFAEAFDVPVVTTLMGIGAIDTTHPL
                     CMRMLGMHGAAFANYAVEDCDLLIAVGARFDDRVAGVPQRFAANARAIAHLDIDRAEI
                     NKVKRVQWSHVGPVREALAALTAWGRRQALAPRHAAWRAELDELRRVHAMNYDRESPL
                     IQPYYVIEEIARRTRGEAIIATGVGQHQMWAAQYSEFRLPRQWLTSGSMGTMGFGLPA
                     AIGAQVANPGRLVIDIDGDASVRMNIGELETVTTYNLPVKVVVLNNFGDGMVKQWQKL
                     FFKGRLSASDKSLHKKDFVKAAQADGFEYAVRLDRKADVPRVVREFLEFAGPAFLEVI
                     IDPDAGVYPMVGPGQTYDKMITGDFIAARSAVPAEAVAPDPSEMF"
     gene            11545..11946
                     /locus_tag="PKN91_06625"
     CDS             11545..11946
                     /locus_tag="PKN91_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNS27782.1"
                     /translation="MVRVSDLDASLRFYCDALGLVDLTRKDNPQGRYTLVFLAAPGDE
                     AAQVELTYNWDPEVYRGGRNFGHLAYAVDDIYAACARLAAHGVTINRPPRDGRMAFVR
                     SPDGISIELLQKGGALAPAEPWSSMPNTGEW"
     gene            11957..14578
                     /locus_tag="PKN91_06630"
     CDS             11957..14578
                     /locus_tag="PKN91_06630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS27783.1"
                     /translation="MPLRTRVAGLLIAIAFGAITFALWAVANRPTPEPPWPARIQGFA
                     FSPFTIEQDPGEQRLPSEADIDRDLELLAGKTNAVRTYSTLGSLGAVPRLADAHEINV
                     ALGAWIDVDADTSERELVAAIAIARSHRNVVRVLIGNEVMLRGDVPAEELYAYLDRAR
                     KAIRQPVGTAEPWHVWLSHAELAEHVDFIAVHLLPYWEGIPVEQAVEYSIRRFEEVRA
                     RFPDKPIIIGEVGWPSNGRTREGAVASMANEALFLRRFLEHAEREGYIYYLMEAFDQP
                     WKATSEGAVGAYWGVYDVERQPKFEFTAPIVRVPQWQVLATVSVLVALFVLALLYFNS
                     ATLRKRGRSFLAVIVFPTATIAVWVFYQFTQQYLTATSVLVGALLCVGMIGVIAVLLA
                     EAHEWAEAQWVVGRRRLAGQPLAEGGEARPRVSIHVPAHNEPPDMLIATLDALARLDY
                     PDFEVLVIDNNTPDEATWRPVEAHCAALGPRFRFFHVAPLAGFKAGALNFALRHTAPA
                     AEVIAVIDSDYIVELGWLRDLAPCFANRYVGIVQAPQDYRDGDENAFKAMCYAEYRGF
                     FHIGMVTRNERNAIIQHGTMTMVRRRLLEVTGGWAEWCITEDAELGLRVFEAGYEAVY
                     TPRSYGRGLIPDTFVDYKKQRFRWAYGAMQILKTHSGLLFSGGGGALTGGQRYHFIAG
                     WLPWVADGFNVLFNVAAIGWSVAMIWDSGRVDPPLAMFALLPLSLFTFKLAKLLHLYH
                     ARVGAGLRQTLAAAIAGLALSHTIGIAVLKGLVTRSEPFFRTPKRAAKQRVRTALNSA
                     REEVLIMLALWLAAWGVAHTPQVASPDRLAWIIVLSIQSVPYAAALVTSLVSAFPLPA
                     RLMGGNRSWREATSSSA"
BASE COUNT         2191 a         5072 c         5167 g         2293 t
ORIGIN      
        1 caacggccag cttctcgtgc gcggcgatca gcgagatcac ggccgggctc agcttgccgg
       61 cacgcgcctc ggtgaggctc gccgagttca gcgagcagat cgcgtcgaga taggcgatgc
      121 cgaccgtcgt gtcgtcgagc tcgcggccgg tcatcaggtg cgtgagcgcg aggcgctgcg
      181 agaccggcgc gaggcgccgg gtttcggcga cctgccggag cagctcttcc gcgttcgagg
      241 cggtgtacgg gtcctggggt gtgtcatgga tgcgtgtcga acccacgcgt acgcgcagcc
      301 ccgcctgcga ggcatagatg ccatcgacga tgttcatgcg cgcgaccagg aaatcgacgg
      361 tcgacgagcc gtagtcctgg tacagctcgt agtcggcgat catcgcgaca ttcagctgct
      421 tgtcgaccac gacggtggcg agggcggtga gttccgcacc gaccgcttca taaagggcca
      481 gcgcattggc agcttcgccg gcagcggcga caccgcagct cactgcgccc tcgtcgatca
      541 gcgcgtcctc gagccggtac acgaccgtgc ccgaggtcgg cggcgtgagc gcggcgtccg
      601 cgaacgcggc gacctgctgc gcaggttcga cggcatagag ctcgcggccg tcggcgatga
      661 gcccgttcag gccctggcgg gtgcgggtga tgcgcgccca ggaccccggc aggccgacga
      721 tgcggccctc gagcgcgacc aggtcgccgc gctccggcgg cacggcgccg cgcagtcgcg
      781 cgttgggttc gagctgcagc tcgaaacgct tgccgtaggc ttcgaaggtg atgcgttcgt
      841 gcccggaggc ggcatcgcgc tgcgcctgca tggcgcgcgc gggttcgagg tagacgatac
      901 gggcgggcgc gcgcgcttcg gcccatggtg ccgccagcac cagtgccagc gcgagtcccc
      961 cggctgacgc cacgccctcg aacctcatcg atcccattat ggaccgttca cccggccgcg
     1021 cgcggtcaaa agtcccggat tcgcgaaatc agcccgcgca gtccttcagc ggcaacatta
     1081 tgtccgcaag gcgtgccggg tcgggccggg cgcgcgcggc gatgagcttg cgcgccgcca
     1141 tctggtcccg ggggctgttc acggtatcga tcgcgatcag ctcgccctcg cgcaggtagc
     1201 aggcggagaa actgcgcgtc gcgggatcgc cgcgcaggac gatgcggtca tgaccctgcg
     1261 acagcccgac gatgaccatt ttcaggtcgt actggtcgga ccagaaccac ggcgtcttgt
     1321 cgtgcacggt ggcgatgccg agcatgttga gtgccgcgct cgtgccctgc tcgaaggcgt
     1381 tgtcgacgga ttcgaggcgg acgccccggt cgtagcgcag gctcggatgg ctcgtgcagt
     1441 cgcccgctgc gaagatgtgg gggtcgttgc tgcggcagta ctcgtcgacg aggatgccgt
     1501 tgtcgcaggc gatgccggct tcccgcgcga gttcgtccac cggcaggacg ccgacgccga
     1561 cgatagcgag atcggccggg tgagcggtgc cgccggcgtc gcgcacggcg cgcaggcggg
     1621 tgtcgccgac gaaggcatcg agcccgctgc gcagcaggat ctgcacgcca tggcgcgcgt
     1681 gctccgcttc atagaagcgc gagacctgct cgcacacgac gcgattcatc acgcggtccg
     1741 cgagctcgat cacggtgacc tcgagtccga gctcgcgcaa tgtcgccgcg gcctcgaggc
     1801 cgatgtagcc gcccccgacg atcaccgcct gccggcccgg ctgcgcggcg gcacgaatgg
     1861 catcgacgtc ggccaacgag cgcagcgtat ggacgcccgg cagatcggca ccggggacgg
     1921 ccagacgtcg cgcccggctg ccggtggcga gcagcagcgt gtcgtaggcc agggtcgcgc
     1981 cgtcgtcgag ctgtacctgg cgtgcggcgc gatcgatgcg cacggcgcgc cgtccgagcc
     2041 gcatcactac gccgtgctcc tcgtagaagg agggttggcg gatcagcagg cgctcgggct
     2101 cgaggctgcc ggtgaggtac tttttcgaca gcggcggtcg ctggtagggc ggcaggggtt
     2161 cgtcaccgat cacggtcagc gattccgtgt atccccgcct gcgcagtgtc tcgacggcct
     2221 gtgccgcaca ctgcccggcg cccacgatca cgatgtgctt cggcatgcac atacgagcat
     2281 actcaccgtg gcaggtgaac ccaagcgcac catggtcggg cacggcgcca gggaacttgc
     2341 gctagaatgg tgcgcggccc ctctcgcgac gcaggggctg gtgcaggaaa tggctgattt
     2401 caggccatgc ggccgctggc atgaaagctg cattttcgtg ccagccagaa cgccagcgac
     2461 gggagtgagt gcatgaaact ggtcaccgcg atcatcaagc cgttcaaact cgacgatgtg
     2521 cgcgcagcgc ttgccgagat cggagtgtcg ggcatgaccg tcaccgaggt gaagggcttc
     2581 ggccgccagc gcggccacac cgagctgtat cgcggcgccg aatacgtcgt cgatttcgtg
     2641 cccaagaccc gcatcgaggt cgcagtcgcc gacaagctgg tcgaccaggt ggtcgatgca
     2701 gtgctcaagg ccgccaagac cggcaaggtc ggcgacggca agatcttcat caccggcatc
     2761 gatcgcgtgg tgcgcatccg caccggcgaa actgacgacg ccgcactctg acgatgtccg
     2821 ccggcgcgac cgggcggcaa gcgctccagc gcgccgtggc cgcgctcgag atcggcagtg
     2881 cgcgccggca catcttcctg tgcgtcggcg gcaagtgtgc cgacgaggac gagcagcagg
     2941 ccagctggaa attcctcaag cggcgcctgc gggaactcgg cctcgtcgac cggccgcagg
     3001 gcgtactgcg caccaaggct gattgcctgc gcgtgtgcgt cggcggaccg atcgcggtgg
     3061 tatacccgga aggaatctgg taccggcgct gcacggccga caacctcgag cgcatcatcc
     3121 aggaacacct gatcggtggg cgcatcgtcg aggatttgcg catcgcgcag accggctcgc
     3181 ccgcgtagac tttcgtcatg ccacgagcca tcgtcatccg cgagaccggc ggtccggaag
     3241 tactgcgcgt cgaggaagtc gccgtggcgg cgcctgcggc cggcgaagtc cagctgcgcc
     3301 acaccgcgat cgggctcaat ttcatcgatg tctacgaccg cagcggcctg tatgcgctgg
     3361 cgctgcctgc cgtgcccggc cgcgaggcgg ccggcgtggt cacggcgctc ggtccgaagg
     3421 tgaagggcct gcgcgtcggc gatcgcgtcg cctatgcact ctccgcgccg ggcgcctacg
     3481 cggaagtgcg caacgtgccg gcggcgcgcg tggtcaagct gccgaagggc gtcaaggacg
     3541 aggtggccgc ggcgatcatg ctgaaggggc tcaccgccca gttcctgctg cgccggacct
     3601 ggcgcgtgcg caagggtgaa tggatcctcg tgcacgcggc cgcaggtggc gtcgggctgc
     3661 tgctgtgcca atgggcgcgg gcgctaggcg cgaaggtgat cggcgtggtc ggcagcgaag
     3721 cgaaggcgaa gctcgcgaag agccacggct gccatcacgt gctgctcgcg gggcgcgacg
     3781 acatcgtcga gcgcgtgaag gccctgacga agggcgcggg cgttcacgtg gtgtacgact
     3841 cggtcggcaa ggacaccttc ttcacctcgc ttgattgcct gcgcccgcgc ggcctgatgg
     3901 tcagcttcgg caatgcgtcg ggacccgtgc ccgccatcgc gccgctcgag ctcgcgaagc
     3961 gcggttcgct gtacctcacc cgaccgaccc tgttcagcta tatcgccact tcccgggaac
     4021 tggccgccgc ggcgcgcgag ctcttcgcca tggtcacgaa gaagaagctg cgcgtgcgca
     4081 tcggtcagcg ctacgcactc gccgacgccg cgcaggccca ccgcgatctc gaggcgcggc
     4141 gcaccaccgg ctcgacggtg ctcgtgccgt gacgcgtgcc ggcgcggcgt gggcggtgct
     4201 cgcgctgacc ggaattgcgc tcttcggcaa ctactacgtc tacgactcga tcggcccgct
     4261 cgccgagcag ctcgcgcgcg agctcggcta ctccgacacg cagatcggca cgctgaacgc
     4321 gatctacagc gtgccgaaca tcgtcctcgt gttcgtcggc ggtgtcctcg tcgaccgcta
     4381 cggaccgggc ctcgtgacct tctggacggc gctggtctgc ctcgcgggcg cattgctcac
     4441 cgcggtgggt acgccgttcg ccgtcatggc ggcgggccgg ctgctgttcg gcatcggcgc
     4501 cgagacgatg atcgtcgcga cgatcgtcgc gctcggcatc tggttcgccg gtcgccagct
     4561 cgccctcgcg atggcgctgt cgatcagctt cgcgcgcctc ggctcataca ccgtcgacct
     4621 ctcgccgctg tgggcccccg acgcctatgc atcaggctgg caggctccgc tgtggctcgc
     4681 ggcgggcgtg atgttcgccg gctgcctcgc cgcctgggtc taccggcgcc tcgaagcgaa
     4741 cgccgccgcg cgcggcgtgc aggtgcaggg gctctcgcgc gagcgcatcg agctgcgcag
     4801 cctcgcgcac ttcaaccgcg gctactggta cgtgctcgcg ctgtgcgtgc tgttctacgc
     4861 cgtgatcttc ccgttccgca gcaccttcgc gatcaagtat ttccagcaca cgggcggcat
     4921 gacactcgag gccgcgagcg tgctgaacag ccacgtgttc ctcgccgccg tgatcgccac
     4981 gccgctgttc ggctgggtga acgaccgcat cggtcgccac ggcctgctgc tcacgctcgg
     5041 cgccgcatcg ctcgtcgcgg cgttctgcgt gctcggcgcg accgacctgt cgcccggcgt
     5101 cagcacggca ttgatcggtg tcgcgttctc gctcgtgccg gcggtgctgt ggccagcggt
     5161 tacgctgctg gtcgcgccgc aacgcctcgg cacggcgtat ggcttcatga ccatgctgca
     5221 gaacatcggc atggccgggg ccaatttcac cgccggtgcg ctcaacgacg ccaatggcgc
     5281 gagcgcctcg catccagatg gctacctgcc gatgatcggc ttcttcgctg cgctgtcggc
     5341 gcttgcgctg gtggccgccg tgctcttctg gcgctacctg tcgcaccatg gggcccgcga
     5401 gccggcgtcg tagcggctga cctgccccgg cggctgcgaa tcggggcggg cggcgcgcct
     5461 tcatggctac aatcggcgta tgagcaatcc gcccctgcgc accgattacc ccgtgttccg
     5521 cgcgctcgcg acgcgctgga tggacaacga ccactacggc cacatcaaca acgtggccta
     5581 ctactcgttc ttcgacaccg cggtcaacgg ctggctcatg gacgcgaccg gcgtcgatat
     5641 ccgcacgctg ccggcgatcg gcctcgtggt cgagacgggt tgccgctatt tcaagccgct
     5701 gtcgtttccc gacgcgctcg aagtcggcct cggcatcgag cggctgggca cgagcagcat
     5761 cacctaccgg ctcgccatct tccgcgccgg cgaagactct gccgcggcgg cgggtcgctt
     5821 cgtgcatgtt tatgtcgatg cgcaaacccg gcaggcgacc gccataccgg acgtgatccg
     5881 cgcggctgcg caggcgctac gcgaaccgat ccggtgaata gaggctgatc ggcaccgacg
     5941 ggtccctccc ggcgatgaga tcgctgatga tccgcccgct cgccggcgcg agggtcacgc
     6001 ccatcatcgc gtgcccggtc gccagcagca cgttgtcggc gcgtcgcgtg cggccgagga
     6061 agggcaggcc gtccggcgtg cagggacgca ggccggccca cggcgtgatg ccggcgaaat
     6121 ccgcgggacc gaactgcggg tagtagcgcg gaatcgcatc gaccatcgcc gcgacccgcc
     6181 gctgatcgat cgagcggtcg agtccgcaca gttccagcgt gccggcgaag cgcagcgcat
     6241 cgcccatggg cgtcacggcg acgaaatctt cggtgagcac cgcgcaggcg tggagtcgtg
     6301 cgcgcggcgc gggcagcatg aagctgtagc ccttgccgga cagcagtggc agcggcaggt
     6361 cgatgtcgcg cgcgagcgcc gcggaccacg cgccgcctgc gatcacgaac tcgtcgccgc
     6421 ccaccacgcc ggcgtcggtg accgcgccgc tcacgcgcgc acccttgcgt tcgagtccgg
     6481 tgacttcggt gttccagtgc aggtcgactc cggcgtcggc aaggccggcg acgagcgtcg
     6541 caaccacgcg ctgtggcacc gtgtgcgcat cgagcggata gcgtactgca ccgacgatag
     6601 ccatgtcgac gcccggttcg agcgcggcgg cggcgtcgcg atcgaggatc tcgaccggca
     6661 tgccgaggcg ctgcgccatc gccgcgacgc ggcgctcctc gcgcatgccg tgcgcgctgc
     6721 gtgcgagcat cagcagcccg tgttcggcga gcggcacgcc ctgtggccac agcagcgtga
     6781 gcgcctcgta gccgcggcgg ccgagcaggg cgaggtcacg cagcgccggc gcggcgagcg
     6841 cgacgcgcga ggcgcgcgcc gcgcacagga actgccagca ccagcgcagg tagtcggggt
     6901 cggcgcgggg cgtcacgcgc agcgggctgc gcggatcggc aagcgagcgc aggcctcgca
     6961 gcaccgcgcc gggcgaggcg agcggaatga aatggctcgg cacgatcatg cctgcattgc
     7021 cgagcgagca gcgatcggta tcggggccgc cgcggtcgag cagcgtgacc acatggccgt
     7081 cctgcgccgc ataccaggcg caggcgagtc cggcgatgcc gccgccgatc acgacgacac
     7141 gcttctgcgg cggcagccgc gcgctcatgg cgcgacccgg atgccgtggg cgagcggatc
     7201 ggcggcatcc atcaggaggg tcgcctccgc ggtcacccag gcctggccgg tgatcgtcgg
     7261 gatgatccgc tcgccgtcgc gctcgtagcg gctctcgaac acgctgccgg tgatgcccgt
     7321 ctgccgccac acctcgccgg gtgcgagccg gccgtcggcc gcgaggcagg ccagtcttgc
     7381 gctggtgccg gtgccgcagg gcgagcggtc gtacgcgccg ccggggcaca gcacgaaatt
     7441 gcgcgcctcg tccgcgagct ccacgtgatc gatctcgacg ccgcccgggc cggtgatgcc
     7501 ggcgcgcgcg agcgcgcggc ggatgcgccc gcagaattcg ccgagcgccg cggcatcggc
     7561 gagcgcgagt cgcctgccgt ggtcctcgca caggaagaac cagttgccgc cccaggcaat
     7621 gtcgccggtg acgggtccat ggccctcgac ctcgacgcgc acgccgcgcg catggcgcca
     7681 ggccggcacg ttgcgcaccg acacggtgtt cgcatcgtgc agcgtggccg tgacgacgcc
     7741 gaccggtgtc tcgaggcgat gctcgccctg cgcgatgcgc ccgagccagg cgagcgtgac
     7801 gatcgtgccg atcgtgccgt ggccgcacat gccgaggtaa ccgacattgt tgaagaagat
     7861 gaccccggcc gtgcagccgg gcgcgagcgg cggggtcagc agcgcgccga ccagcacgtc
     7921 ggaaccgcgc ggctcgttga cgatggcgct gcggaagtgg tcgtggttgc gccgcaggtc
     7981 ttcgacgcgc gcggcgaagg atccggtgag cgccggcccc ccgtcgatca ccacacgcgt
     8041 cggctcaccg ccggtgtgcg aatcgacgac ccgcacgcgc atcagcgtcg gcggcgccgc
     8101 ggcaggggcg gcagcttcgg ccggtgttcg aggccggcct cgatgatgcc gaggatgcgg
     8161 cggcgctcct cgccgaccag tacgcgtcgc ggtgcgcgca cccattcggc gccgaggccg
     8221 gccgcctgga ccgcgagctt gatgtactgc acgaacttga cgtgcgtgtc gaggtgcgcg
     8281 agcggcgtga accagcggta aagccgcagg gcagcatcga ccgcgccggc caccgcgagg
     8341 tcccatagcc gctggttctc gcgcgggaag gcgaggccga tgccggcgat ccagccgtcg
     8401 gcgccgagca ccgcactctc gagcgcgaga tcatcgacgc ccgcgaacag cacgtagcgg
     8461 tcgccgagcg cattgcgcag gtcggtgatg cggcgcacgt cccccgagga ttccttgatc
     8521 gcgaccaggt tcgaaacgcc ggcgaggtcg acgaacatcg ccggagtgat gtcgacgtgg
     8581 taggcgagcg gattgttgta gcagatgatc ggcagctgcg tggcgcgtgc gacggcccgg
     8641 tagtgcagca gcgtctcccg cgggtcggcg cggtagacca tggccggcag cagcatcagg
     8701 ccatcgacgc cgaggcgctc catgtcgcgc gcgaaggcga tggcggcggc agtgctcgcc
     8761 tcgaatacgg tggcgatcac cggcacgcgc cctgccgcca cctcgagcgc ggcggccacg
     8821 accgcgcgtt tctccgccgg ggcgagcgtg gggttctcgc cgagcgagcc gagcatcacg
     8881 aggcccgagg cgccgcttgc gaccatcgcc tcgatgtggc gcgatccggc ttcgaggtcg
     8941 agcgattcat cgcggcggaa ctgcgtggtc atcgccggga acacatcgcg ccacggaacc
     9001 gtcatgagcc gcacccgccg cgcatcgcgc gccgctcagc cgacgagctt gaggttgcgc
     9061 gccgcacggc tgacgccgag cgcgccgctg cgcaccactt cgaccgcctc cgtgtagtgc
     9121 gcgagttcgg cgatgaaatc gctcacctcg gcttcgctgg cggtcagctc gacgacgaag
     9181 ccgtcgggcg ccgggtcgag cacgcgcccg ccgcgacgga cgacgtagcc gcgcacgaca
     9241 tcggtctgct cgggccgcag gcgcagtttc gccagcacga gctcgcgctc gatgtgctca
     9301 ccgtcggtga gatcctcgac cgcgacgaca tcgatcagct tcagcagctg gttggcgatc
     9361 tgctgcatca ccgcctccga gccccgtgtg acgagcgtga tgcgcgagac ggtgggatcg
     9421 tcggtggcgg cgactgacag cgactcgatg ttgtagccgc gggtggaaaa gaggcccgcg
     9481 acgcgcgtga gcgcgcccgc ctcgttctgc aggaggatgg caatgatgtg gcgcatggaa
     9541 ttccggggaa attgagcact tggaagaata ctgcatgcgc gcccgtgcat ggcggttttg
     9601 cgcgccgggg ccaaactgtt agtctttcag gtcaccccca cgcagagcga aaccagcccc
     9661 cgatgtcaga cacggtcata atgccccgcc cgcaggccca cccgctggcc ggccaggcca
     9721 tgaccggcgc gcagatcatc gtgcaggtca tcgccgacga gggcgtctcg gccatcttcg
     9781 gctacagcgg cggcgcgatc ctgccgacct atgacgcggt gttcctgtac aacgaggccc
     9841 gccagctcga cggcggccag cagatgccgc tgatcgtgcc ggcgaacgag cagggggcgg
     9901 gcttcatggc ggcgggctat gcgcgctcga gcggtcgcgt cggcgtcgcg ctcgtgacct
     9961 cgggaccggg cgcgaccaac accgtcacgc cgatccgcga cagcatggcc gattcggtgc
    10021 ccatggtcgt gatctgcggc caggtgccca ccgcggccat cggttccgac gccttccagg
    10081 aggcgccggt cagctcgatc atgggcgccg tggccaagca cgtgttcctc gtcaccgagc
    10141 ccgagaagct cgaggccacg gtacgcacgg ccttcgaggt ggcgcgcacc ggccggccgg
    10201 ggccggtcgt catcgacgtg ccgaaggacg tgcagaactg gcagggccag ttccacggcc
    10261 agggcacgct gccgatcccc ggttatcgcc gtcgcatgga cagcgctaaa cgctcggcca
    10321 tggccgatgc cgacgcggcg cgtttcttcg aactgctcgc cgcctccgag cgtccgctca
    10381 tctacgccgg cggcggtgtg gtcaatggct cggcgtcgga ggcgttgcgg gagttcgccg
    10441 aagccttcga cgtgccggtg gtcaccaccc tgatgggcat cggcgcgatc gacaccaccc
    10501 atccgctgtg catgcgcatg ctcggcatgc acggcgccgc gttcgcgaac tacgcggtcg
    10561 aggactgcga cctgctgatc gcggtcggcg cgcgcttcga cgaccgtgtc gccggcgtgc
    10621 cgcagcgttt cgcggccaat gcacgcgcga tcgcgcacct cgacatcgac cgcgccgaga
    10681 tcaacaaggt caaacgcgtg caatggagcc atgtcggccc ggtgcgcgag gcgctcgccg
    10741 cgctgaccgc ctggggccgc aggcaggcgc tcgccccgcg gcacgcggcg tggcgcgcgg
    10801 agctcgacga attgcgccgc gtgcacgcga tgaactacga ccgcgagagc ccgctgatcc
    10861 agccgtacta cgtcatcgag gagatcgcgc ggcgcacccg cggcgaggcg atcatcgcga
    10921 ccggcgtcgg ccagcaccag atgtgggcgg cccagtacag cgagttccgc ctgccgcgcc
    10981 agtggctcac ctccggcagc atgggcacga tgggcttcgg cctgccggcg gccatcggcg
    11041 cccaggtcgc caatcccggc cggctggtga tcgacatcga cggcgatgcg agcgtgcgca
    11101 tgaacatcgg cgagctcgag accgtcacca cctacaacct gccggtcaag gtcgtggtgc
    11161 tgaacaactt cggcgacggc atggtcaagc agtggcagaa gctgttcttc aagggccggc
    11221 tgtcggcgag cgacaagtcg ctgcacaaga aggatttcgt caaggccgcg caggccgacg
    11281 gcttcgagta cgcggtacgt ctcgatcgca aggccgatgt gccgcgcgtg gtgcgcgagt
    11341 tcctcgaatt cgcaggcccc gccttcctcg aggtcatcat cgatccggac gcgggcgtct
    11401 atccgatggt tgggccgggg cagacgtacg acaagatgat aaccggtgat ttcatcgccg
    11461 cgcgcagtgc agtgccggcc gaagcggtgg cgccggatcc gtccgaaatg ttctgagggt
    11521 gcgcgcctga aatacctgca cacgatggtg cgagtctccg atctcgacgc ttcgctgcgt
    11581 ttctattgcg acgcgctcgg gctcgtcgac ctcacgcgca aggacaatcc ccaggggcgt
    11641 tacaccctgg tgtttctcgc cgcgcctggc gatgaggcgg cgcaggtcga attgacctac
    11701 aactgggatc ccgaggtcta tcgcggcggg cgcaatttcg gccatctcgc ctacgcggtc
    11761 gatgacatct acgccgcctg cgcgcgcctc gccgcgcatg gcgtgacgat caaccggcca
    11821 ccgcgcgacg gtcgcatggc cttcgtgcgc tcgcccgacg gcatttcgat cgaactgctg
    11881 cagaagggcg gcgcgctcgc gcccgccgag ccgtggagct cgatgccgaa caccggcgag
    11941 tggtgagccg catgttatgc cgctgcgtac tcgtgtcgcg ggactgctga tcgcgatcgc
    12001 cttcggggcg atcaccttcg cgctgtgggc ggtggccaat cgcccgaccc cggagccgcc
    12061 gtggcctgcg cgcatccagg gcttcgcgtt ctcgcccttc accatcgagc aggatcccgg
    12121 cgagcaacgc ctgccgtccg aggccgacat cgatcgcgac ctggaactgc tcgccggcaa
    12181 gaccaacgcc gtgcgcacct acagcaccct cggctcgctc ggtgcggtgc cgcggctcgc
    12241 ggatgcgcac gaaatcaacg tggcactcgg cgcctggatc gatgtggacg ccgacaccag
    12301 cgagcgcgag ctcgtggccg cgatcgcgat cgcgcgcagc caccgcaatg tcgtgcgcgt
    12361 gctgatcggc aacgaggtca tgctgcgcgg cgacgtgccg gccgaggagc tgtacgccta
    12421 tctcgaccgc gcgcgcaagg cgatccgcca gcccgtcggc accgccgagc cctggcacgt
    12481 ctggctctcg cacgcggagc tcgccgaaca cgtcgatttc attgcggtgc acctgctgcc
    12541 ttactgggag ggaatcccgg tcgagcaggc ggtcgagtac tcgatccggc gtttcgagga
    12601 ggtgcgggcg cggtttcctg acaaaccaat catcatcggc gaggtgggct ggcccagcaa
    12661 tggccgcacg cgcgagggcg ccgtcgcctc gatggcgaac gaggccctgt tcctgcgccg
    12721 cttcctcgag cacgccgaga gggagggcta catctactat ctgatggagg ccttcgacca
    12781 gccgtggaag gcgacgtccg agggcgcggt gggcgcgtac tggggcgtct acgatgtcga
    12841 gcgccagccg aagttcgagt tcaccgcgcc gatcgtgcgg gtgccgcagt ggcaagtgct
    12901 cgccacggtg tcggtgctgg tcgcattgtt cgtgctggcg ctgctgtatt tcaacagcgc
    12961 gacgctgcgc aagcgcggcc gcagcttcct cgcggtgatc gtgttcccga ccgcgacgat
    13021 cgccgtgtgg gtgttctacc agttcacgca gcagtacctc accgcgacca gcgtcctcgt
    13081 cggcgcgctg ctctgcgtcg gcatgatcgg cgtgatcgcc gtgctgctcg ccgaagcgca
    13141 cgaatgggcg gaggcgcagt gggtggtcgg gcgacggcgt ctcgccgggc agccgctcgc
    13201 cgagggcggc gaggcgcgcc ccagggtgtc gatccacgtg ccggcccaca acgagccgcc
    13261 cgacatgctg atcgcgacgc tcgatgcgct cgcgcggctc gactatcccg acttcgaagt
    13321 gctggtgatc gacaacaaca cgccggacga ggcgacctgg cgcccggtcg aggcgcactg
    13381 cgcggcgctc ggcccgcgct ttcgtttctt ccacgtcgcc ccgcttgcgg gtttcaaggc
    13441 gggtgcgctc aacttcgcgc tgcgccacac cgcgcccgcc gccgaggtca tcgcggtgat
    13501 cgacagcgac tacatcgtcg agctcggctg gctgcgcgat ctggcgcctt gcttcgccaa
    13561 ccgctatgtc ggcatcgtgc aggcgccgca ggactatcgc gacggcgacg agaacgcgtt
    13621 caaggccatg tgctacgccg agtaccgcgg gttcttccac atcggcatgg tcacccgcaa
    13681 cgagcgcaat gcgatcatcc agcacggcac gatgacgatg gtgcgccggc gcctgctcga
    13741 ggtgaccggc ggctgggccg agtggtgcat taccgaggac gccgaactcg gcctgcgcgt
    13801 gttcgaggcc ggttacgaag ccgtctatac gccgcgctcc tacgggcgcg gcctgatccc
    13861 cgacaccttc gtcgactaca agaagcagcg cttccgctgg gcctacggcg cgatgcagat
    13921 cctgaagacc cattcgggcc tgctgttctc gggcggcggg ggcgcactga cgggcggcca
    13981 gcgctatcac ttcatcgccg gctggctgcc gtgggtggcc gacggcttca acgtgctgtt
    14041 caacgttgcg gcgatcggct ggtcggtcgc gatgatctgg gactccggcc gggtcgatcc
    14101 gccgctcgcg atgttcgcgc tgctgccgct gtcgctgttc accttcaagc tcgcgaagct
    14161 gctgcacctc taccatgcgc gcgtcggcgc gggcctgcgc cagaccctcg cggcggcgat
    14221 cgccgggctc gcgctgtcac atacgatcgg catcgcggtg ctcaaggggc tcgtgacgcg
    14281 cagtgagccg tttttccgta cgccgaagcg ggccgcgaag cagcgcgtgc gcacggcgct
    14341 caattcggcg cgcgaagagg tcctgatcat gctcgcgctg tggctggcgg cctggggcgt
    14401 cgcgcacacg ccgcaggtcg ccagccctga ccggctcgcg tggatcatcg tgctgtcgat
    14461 ccagtcggtg ccctacgcgg cggcgctcgt gacctcgctc gtcagcgcct tcccgctgcc
    14521 cgcgcgcctg atgggcggca accgcagctg gcgcgaggct acttcatcgt cggcatgacg
    14581 aattcggcgg tctcggcgcc gctcgtcggc cagcgcgcgg tgatcgcctt cagtcgcgtg
    14641 tagaagcgca caccctccgg gccgtgcacg tgcatgtcgc cgaacagcga gcgcttccag
    14701 ccgccgaagc tgtggaacgc cat
//
LOCUS       DAOQVY010000053        14449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_4730_length_14449_cov_5.290677, whole genome shotgun sequence.
ACCESSION   DAOQVY010000053 DAOQVY010000000
VERSION     DAOQVY010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14449
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4730_length_14449_cov_5.290677"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..996)
                     /gene="ispH"
                     /locus_tag="PKN91_06635"
                     /gene_synonym="lytB"
     CDS             complement(49..996)
                     /gene="ispH"
                     /locus_tag="PKN91_06635"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNS27784.1"
                     /translation="MKIRLANPRGFCAGVDRAIEIVGRAIELFGAPIYVRHEVVHNRH
                     VVERLRELGAVFVDELDEVPDGATVVFSAHGVSRAVEDEARRRALNVFDATCPLVTKV
                     HMEVARFAHEAREVILIGHEGHPEVEGTMGRFDASAGGHIILVESVEDARTVPVRDPS
                     RLAFVTQTTLSVDDTAQIVAVLRERFPQLASPRKEDICYATQNRQDAVKQLLVECDAL
                     VVVGSVTSSNSNRLRELADRAGVPGYLVDGPDDLRPEWFSASSVVGVTAGASAPEVLV
                     QQVVERLRSWGGEVADEMHGREEHVVFALPKALRAKPPS"
     gene            complement(993..1484)
                     /gene="lspA"
                     /locus_tag="PKN91_06640"
     CDS             complement(993..1484)
                     /gene="lspA"
                     /locus_tag="PKN91_06640"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNS27785.1"
                     /translation="MSGWRWLPLSALIVALDQATKLWVERSFQLYESVTLLPVFDLTY
                     LRNTGAAFSFLAGAGGWQRWFFSLLAVGVSVGIIVWLRGVHARAQAWLAAGLALILGG
                     ALGNVIDRLRLGYVVDFVHVHWQEYYFPAFNVADAAITVGAACVIIDTLLESRRARAR
                     GPA"
     gene            complement(1481..4288)
                     /gene="ileS"
                     /locus_tag="PKN91_06645"
     CDS             complement(1481..4288)
                     /gene="ileS"
                     /locus_tag="PKN91_06645"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNS27786.1"
                     /translation="MADYKQTINLPQTGFPMKADLAQREPKMLAEWEAGDLYGKIRAA
                     ARGRPRFVLHDGPPYANGAIHIGHAVNKILKDIIVKSRSLDGFDAPYIPGWDCHGLPI
                     EHAIEKKHGKPGQKLDANAFRAACRAFAQQQIDLQRTDFKRLGVLGDWDRPYLTMDAA
                     YEAEQIRAFGRIVHNGHLYRGAKPVYWCLDCRSALAEAEVEYEQHTSPAIDVEFRVAD
                     LRDLERRIGQPAGGLGSMPVSLVIWTTTPWTLPANEAVALRDSFRYSLVEVESAVGRE
                     RLILASELRDACLARYGLAPISVLAEFDGVALERLELEHPFLEKRVPVILGEHVTLEA
                     GTGAVHTAPAHGQEDFIVGQRYGLPVVNPVGPDGRFHSNTPFVANLRVDAANAVIVEA
                     LAGAHRLLQHVAYAHSYPHCWRHKTPVIFRATPQWFISMEQRNLRAHALRDIKAVKWT
                     PAWGEQRITGMIENRPDWCISRQRTWGVPITLFVHKETGALHPDTLALIDKAADHVAR
                     GGIEAWFALDAADLIGADSATYEKVTDVMDVWADSGLSFECVGAARPEIAAPVDLYLE
                     GSDQHRGWFHSSLLMSEALHERAPYKGVLTHGFTVDEKGRKMSKSLGNVILPQKVVNS
                     LGADVLRLWVASTDYANEIAVSDEILKRAADSYRRMRNTVRFLLGNLHGFDPQADALP
                     VGELVALDAWAMRRTRELQEEIVAAYRDYAFHLIYQKVHNFCIVDLGGLYLDVLKDRL
                     YTTPAKSHARRSAQTAMFHIAEAMVRWLAPILSFTSEEIWKFMPGKRGESVQCETWYV
                     MPEAPAADIDWEALIALRAAVMRELEKLREAGVIGAPLEAQVDVWCQPAQLARHAALG
                     DELRFLLITSAARVHQGEAPADAVVVADGVAIRVAPVEAPKCVRCWHRLPDVGSDAEH
                     PELCGRCVTNLGTEGERRRYA"
     gene            complement(4281..5231)
                     /locus_tag="PKN91_06650"
     CDS             complement(4281..5231)
                     /locus_tag="PKN91_06650"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNS27787.1"
                     /translation="MKLIRNWTGLRTVRRGPVVAVGAFDGLHLGHHELIERARRHAEV
                     RGTGAMMLTFEPLPREFLQPQSPPARLTNFRERWRLLEGSGLQYLCVLRFDERLRSLS
                     GEDFAGALAATCAASVVVVGHDFRFGCGGTATAQTLEALGRRHGFEVEVVAPVGSGER
                     ISSTAVRTGLASGDFAAAARLLGRPYTMRGRVSRGERLGRTLGFPTANLRLKRRRAPL
                     GGIFAVRVHGVAANPWPGVASLGTRPTVDGVEPWLETFLFDFDADLYGREIEVEFVAK
                     IRDEERFASLGAMVEKMHEDARDARRLLAGLKLTARNPSG"
     gene            complement(5234..6796)
                     /gene="murJ"
                     /locus_tag="PKN91_06655"
     CDS             complement(5234..6796)
                     /gene="murJ"
                     /locus_tag="PKN91_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNS27788.1"
                     /translation="MSRAFLRSTGVVGATTLVSRITGLLRDMALSQVFGAGLLMDAFL
                     VAYKIPNFLRRLFAEGAFSQAFVPVISEFRVKRAQGEVRDLVSGTAGTLGTVLLVVTL
                     IGVIGAPVLILLFAPGFTQHGDKYDLAVDMLRWTFPYLFFVSLTALCSGVLNSYGRFA
                     VPAFTQVIMNVVMIFFAVVLAANSANPGIVLAIGVFVSGVLQVLFQLPAVAKLGLLAW
                     PRWQPRGEGVRRIGRLMVPGIVGSSMAQVSLLLDTIIASFLMSGSISWLYFADRLMEF
                     PLGVFSIALATVILPGLSAQHAEASMDRFAGTLDWALRLTVLLAAPAAVGLLFFAGPL
                     TATVFGYGQFSSRDVQMAAYALMAYSWGLLMFSIVKVLAPGYYARQDTRTPVRVAIIG
                     FAVTMSLNVVVVLPAKWLGFPIPHVLLATSTCAGAAVNAFLLWRGLRRAGVYRASAGW
                     GRFLARVLTANAAMAALLWWFTGDLDTWTAMGALQRALRCAIGITAAAGLYFAVLFVS
                     GLRYGDLRNRPI"
     gene            6939..7202
                     /gene="rpsT"
                     /locus_tag="PKN91_06660"
     CDS             6939..7202
                     /gene="rpsT"
                     /locus_tag="PKN91_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNS27789.1"
                     /translation="MANSKSAERAARRAEKHRARNVTLRSSTRTTLRKVNSAIGTGDK
                     AAATAAYRDAVSAVDALAAKGKIHRNKAARHKSRLSARVKSMQ"
     gene            complement(7186..8226)
                     /gene="obgE"
                     /locus_tag="PKN91_06665"
     CDS             complement(7186..8226)
                     /gene="obgE"
                     /locus_tag="PKN91_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNS27790.1"
                     /translation="MKFVDEARIKVQAGNGGRGSTSFRREKFVPFGGPDGGDGGNGGD
                     VLLRAAEGINTLADFRIERTFKGEHGQPGSGNGCTGRSGVDACVVVPVGTIVRDAETQ
                     ETLGDLTRPGQELLVARGGKGGWGNTRFKSSTNRAPRQFGPGLPGERRTLELELKVIA
                     DVGLLGLPNAGKSTLISAVSAARPKIADYPFTTLYPNLGVVYVGQHRSFVMADIPGLI
                     EGAAEGAGLGIRFLKHLQRTRVLLHLVDIAPIDPDADPVRDARAIVAELKKFSPELAA
                     KPRWLVLNKRDLLPAHEAQARAADIARRLRYKGPKFLISAVTGEGTKELTEAVMQYIE
                     AQAARPETTASI"
     gene            complement(8315..8572)
                     /gene="rpmA"
                     /locus_tag="PKN91_06670"
     CDS             complement(8315..8572)
                     /gene="rpmA"
                     /locus_tag="PKN91_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNS27791.1"
                     /translation="MAHKKAGGSTRNGRESHSKRLGVKKFGGESVLAGNILVRQRGTE
                     YHAGPNVGIGTDHTLFALKNGTVRFAVKGAEQRRFVSVDAE"
     gene            complement(8611..8919)
                     /gene="rplU"
                     /locus_tag="PKN91_06675"
     CDS             complement(8611..8919)
                     /gene="rplU"
                     /locus_tag="PKN91_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNS27792.1"
                     /translation="MYAVIATGGKQYRVEQGQVLRIEKLEAEPGATVTFDKVLLVGNG
                     SDIKVGAPLLSGATVTGTVERHGKAAKVAIVKFRRRKHYLRMKNHRQPFTEVRVTAIG
                     "
     gene            9116..10084
                     /locus_tag="PKN91_06680"
     CDS             9116..10084
                     /locus_tag="PKN91_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNS27793.1"
                     /translation="MTLDEIRALAQPDLAAVDDVIRAGLHSEVPLANQIAEHIIAGGG
                     KRLRPLLVVLAGRAAGAAGTRHIEAAAFIEFIHTATLLHDDVVDGSELRRGRHTANQV
                     FGNQASVLVGDFVYTRAFQMMAAIGSQRVIEIMAMATNVIAEGEVLQLMNAHDADTTE
                     QRYLDVIYRKTAKLFEAGAEVAAVLSNAPATVQDALRRYGCHLGTAYQLIDDVLDYRS
                     DPDARGKNLGDDLAEGKPTLPLIRALSVGDEVQRAQIRAAIEHGGIEHLDAIIATIEA
                     TGGLDYAQGLARREADQAIAALQDVPPSPYRDALAALAAFAVAHQT"
     gene            complement(10183..10731)
                     /locus_tag="PKN91_06685"
     CDS             complement(10183..10731)
                     /locus_tag="PKN91_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27794.1"
                     /translation="MTKLKPSARFAALAVAIAGMFALGTDAALAQGQNGRGVGKQSVG
                     VSYYKVTPGKQDEWLALYKKYHKPIVEYGRRYSGGTSKIYAAGNHAPGAPWDFAIISL
                     GPKPGEVVAKPKEKELSRAELIRKLYPDIEDYVRGERARWALTIDHWDERFVELDPND
                     EPFSVYYPLVTKEAAEAAAKGN"
     gene            complement(10752..13676)
                     /locus_tag="PKN91_06690"
     CDS             complement(10752..13676)
                     /locus_tag="PKN91_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS27795.1"
                     /translation="MNRSIPGAASLVAAALVFGSAAALADADAVAVTAPAADSADPAG
                     SVSLEPLQEVAITGSRIVRKDYVGNSPLTTVTMEALEASGAPTLEVALNALPQFAIGA
                     NATVAGFSGTGQATLNLRGLGPTRNLVLLDGRRLQPADVLGVVDINTIPSALVQNVEV
                     ITGGASAVYGSDAIAGVVNLQINPRFEGVQIDTQYNVFGEGDGATLDTSLTVGGNFAD
                     DRGNAVVSFSYLDREDVGYMSRDFFARARGGTDFRIPTGIYRTNVNGANLPTQAAVDQ
                     VFAQYGAAAGRVPNVAVLGYNDDGTLFAASHGPFNYRGPDGVLFDTGTQLNNLNLVSR
                     LQVPLTRYSAFGRGSYDLSEKLTAYAQVYYTTYESLTNAEAGNDAFLVPVTNPFIPAD
                     LATILASRPDPDAPFRFEKRFHSEAGAREFNRQFDVYQLTAGLKGPIEAIDGSWDVYA
                     ASGNTQKIETNAGSVLVNNLKALLNAPDGGASLCAGGYNPFGFSQLSPECRNFLVATP
                     ISETNLEQNVIEATLQGRVLELPAGDLRFAAGAGFRSQSYDYQPDHDLALGNVVGVFR
                     TGESRGRSDVAEVYGELLVPLLRDRLLARQLDLNVAYRYSDYDLAGGVDTYKADFSWE
                     LVDSVRLRGGYQHAVRAPSVGELFVAPNVSIPGIGNVASGGGDPCHYQSQARAADPAA
                     ARALCLAQGIAPGIIDTFVNTQDEILATSSGNTSLRPESGDTRTFGVVWTSPFESEYL
                     ARLQVSADYYDIEIEDVIGTIGAAQILPKCFNQDGSNPALSNSNFYCSQITRDPATGF
                     IANVGQPTLNLGSYRTKGVDFQVDWAWGPFSVNTVASWLDSFEVQLQKGSNVLEYGGS
                     VGTPSATQPGSLPDWKAVTRFQYATPAITAGLRWRYLDAMRHSSLVTNPNSTVPGVSA
                     FNYFDLYGSWQVSDLVRVHGGVNNVDDREPPLVGTSLGSTQSSTYDIYGRQYYLAVRF
                     TF"
     gene            complement(13776..>14449)
                     /locus_tag="PKN91_06695"
     CDS             complement(13776..>14449)
                     /locus_tag="PKN91_06695"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HNS27796.1"
                     /translation="RRQTIAKRLVEAQHTASTMRLVDSLAEQALLESILEASKPPLPP
                     GTAKLHYLLATPFRYRPRSGSRFRAPFDGGVWYGAEALRTALAEKSYWRLRFLLDSPG
                     TPDLKPVTHTAFAASVRGKSIDLTVLPLSKDRAAWTHHSDYTRTQALAAAAREAQVEL
                     IRYESVRDPEHAACVAALVPGVFGRGAPKQQQTWFIAASRARVRCVAGARGGETWEFD
                     ARTLL"
BASE COUNT         2475 a         4921 c         4816 g         2237 t
ORIGIN      
        1 cgagaggagc cggacatgcg gacgccttgg gtcgccgagt gcgggccctc aggaaggtgg
       61 cttcgcgcgc agcgccttcg ggagggcaaa gaccacatgc tcttcgcgcc cgtgcatttc
      121 atcggcgacc tcgccgcccc agctgcgcag acgctccacg acctgctgga cgaggacttc
      181 cggcgctgac gcacccgccg tgacgccgac cacactgctg gctgagaacc actccgggcg
      241 caggtcatcc ggaccatcga ccaggtagcc gggcacgccg gcgcgatcgg cgagttcgcg
      301 cagccggttc gagttcgaac tcgtcaccga gccgacgacg accagcgcgt cgcactcgac
      361 gagaagctgc ttgaccgcat cctggcggtt ctgcgtcgca tagcagatgt cttccttgcg
      421 cggactcgcg agctggggaa accgttcgcg cagcaccgcg acgatctgcg cggtgtcgtc
      481 gaccgagagc gtcgtctgcg tcacgaaagc gagccgggac gggtcacgca cgggcacggt
      541 gcgcgcatcc tcgaccgatt cgaccaggat gatatgcccg ccagcgctcg cgtcgaagcg
      601 acccatcgtg ccttcgacct ccgggtgccc ttcgtggccg atcaggatca cctcacgcgc
      661 ctcgtgcgca aaccgcgcca cttccatgtg caccttggtc acgagcgggc aggtggcatc
      721 gaatacgttg agtgcacgcc gccgggcctc gtcctcgaca gcgcgcgaga caccgtgggc
      781 ggagaagacc actgtggcgc catccggcac ttcgtcgagc tcatcgacga acaccgcgcc
      841 gagttcgcgc aggcgctcca cgacgtggcg gttgtgaacg acctcgtgcc tcacgtagat
      901 cggcgcaccg aacagctcta tcgcgcgccc gacgatctcg atcgcgcggt ccacgccggc
      961 gcagaagccg cgcggattgg cgagccggat cttcatgccg gcccgcgcgc gcgcgcgcgc
     1021 cgagactcga gcagcgtgtc gatgatcacg caggccgccc cgaccgtgat cgccgcgtcg
     1081 gcgacgttga aggccggaaa gtagtattcc tgccagtgca cgtgcacgaa gtcgaccacg
     1141 taaccgagcc gcaggcggtc gatcacgttg ccgagggcgc cgccgaggat cagcgccagg
     1201 cccgcggcga gccaggcctg cgcgcgcgcg tgcacgcccc tcagccagac gatgatgccg
     1261 acactgacac cgacggcgag cagcgagaag aaccaccgct gccagccgcc cgccccggca
     1321 aggaagctga aggccgcgcc ggtgttgcgc agataggtca ggtcgaacac aggcagcagc
     1381 gtgacgcttt cgtacaactg gaagctgcgc tcgacccaca acttggtcgc ctggtcgagt
     1441 gcgacgatga gtgcggacaa tggcaaccag cgccagccgc tcatgcgtag cgcctcctct
     1501 cgccttcggt gccgaggttc gtgacgcaac gaccacacag ttccggatgc tccgcatccg
     1561 atccgacatc cggcaagcgg tgccagcagc gcacgcactt cggcgcctcg acgggcgcca
     1621 cgcggatcgc gaccccgtcg gcgaccacca cggcatccgc cggggcctcg ccctggtgta
     1681 cccgcgcggc cgaggtgatc agcagaaagc gcagttcgtc gccgagcgcc gcgtgccgcg
     1741 cgagctgcgc aggctggcac cacacatcga cctgggcctc gagaggcgcg ccgatcacgc
     1801 cggcctcacg cagcttctcg agttcgcgca tcaccgccgc acgcaatgcg atcaatgcct
     1861 cccagtcgat gtcggccgcc ggcgcctcgg gcatcacgta ccaggtctcg cactgcacgg
     1921 actcgccgcg cttgccgggc atgaacttcc agatctcctc ggacgtgaac gacaggatcg
     1981 gcgcgagcca gcgcaccatg gcctcggcga tgtggaacat cgcggtctgc gccgaacggc
     2041 gcgcgtggct cttcgcgggt gtcgtgtaca ggcggtcctt cagcacgtcg agatagaggc
     2101 cgccgaggtc gacgatgcag aagttgtgca ccttctggta gatgaggtgg aacgcgtagt
     2161 cgcgataggc cgcgacgatc tcctcctgca gttcgcgggt gcgccgcatc gcccaggcat
     2221 cgagcgccac gagttcgccc accggcagcg catccgcctg cggatcgaag ccgtgcaggt
     2281 tgccgagcag gaaacgcacg gtattgcgca tgcggcggta cgaatcggcg gcgcgcttca
     2341 ggatctcgtc ggagacggcg atctcgttgg cgtagtcggt cgaggccacc cacagtctca
     2401 ggacatcggc cccgaggctg ttgacgacct tctgcggcag gatcacgttg ccgagcgact
     2461 tcgacatctt gcgacccttt tcgtccacgg tgaacccgtg ggtcagcacg cccttgtagg
     2521 gcgcgcgctc gtgcaacgcc tcggacatca gcagcgaact gtggaaccag ccgcggtgct
     2581 ggtccgagcc ctcgagatag agatcgaccg gggcggcgat ctcgggacgc gcggcgccga
     2641 cgcactcgaa ggacaggccc gaatcggccc acacgtccat cacgtcggtc accttctcgt
     2701 aggtcgcact gtcggcaccg atcaggtcgg cggcgtcgag ggcaaaccag gcctcgatgc
     2761 caccccgcgc gacatgatcg gccgccttgt cgatcagcgc gagtgtgtcg ggatgcagcg
     2821 cgcccgtctc cttgtgcacg aacagcgtga tcggcacgcc ccaggtccgc tggcgcgaga
     2881 tgcaccagtc ggggcgattc tcgatcatgc cggtgatgcg ctgttcgccc catgcgggag
     2941 tccacttcac cgccttgatg tcacgcagcg cgtgcgcgcg caggttgcgc tgctccatgc
     3001 tgatgaacca ctgcggggtg gcgcggaaga tcaccggcgt cttgtggcgc cagcaatgcg
     3061 gatagctgtg cgcgtacgcc acgtgctgca gcagccggtg cgccccggcg agcgcctcga
     3121 cgatcaccgc attggccgca tcgacgcgca ggttggcgac gaacggtgtg ttcgaatgga
     3181 aacgcccgtc ggggcccacg ggattcacca ccgggaggcc atagcgttgc ccgacgatga
     3241 agtcctcctg gccgtgggcg ggcgcggtgt gcaccgcgcc ggtaccggct tcgagcgtca
     3301 cgtgctcgcc gaggatcacc ggtacgcgct tctcgaggaa cgggtgctcg agttcgaggc
     3361 gctcgagcgc gacaccgtcg aattccgcca gcacggagat cggcgcgagg ccgtagcgtg
     3421 ccaggcacgc atcgcgcaat tcgctcgcaa ggatcagccg ttcgcgcccg acggcggatt
     3481 caacttcgac caggctgtag cggaacgagt cacgcaatgc gaccgcctcg ttggccggca
     3541 gcgtccatgg cgtcgtggtc cagatcacga gcgacaccgg catgctgccg agcccacccg
     3601 cgggctggcc gatgcgtcgc tcgaggtcgc gcaggtcggc gacgcgaaac tccacgtcga
     3661 tcgccggcga ggtgtgctgt tcgtactcga cttccgcctc ggcaagcgcc gagcggcaat
     3721 cgaggcacca gtacacgggt ttcgcgccgc gatacaggtg gccgttgtgc acgatccgtc
     3781 cgaaggcacg gatctgctcg gcctcgtatg ccgcgtccat cgtcaggtac ggtctgtccc
     3841 agtcgcccag cacgccgagg cgcttgaagt ccgtgcgctg caggtcgatc tgctgctgcg
     3901 cgaacgcgcg gcaggcggca cggaacgcgt tggcgtcgag cttctggccc ggcttgccgt
     3961 gcttcttctc gatggcgtgc tcgatcggca ggccatgaca gtcccagccc gggatgtacg
     4021 gtgcgtcgaa accatcgagc gagcgcgact tgacgatgat gtccttcagg atcttgttga
     4081 ccgcgtgtcc gatgtggatc gcgccattcg cgtacggcgg gccgtcgtgc agcacgaagc
     4141 gcggccgccc gcgcgccgcc gcgcggatct tgccgtacag gtcgcccgcc tcccactccg
     4201 cgagcatctt cggctcccgc tgcgcgagat cggccttcat cggaaagccg gtctgcggga
     4261 gattgatcgt ctgcttgtaa tcagccactc gggttccttg ccgtcaactt gaggccagcg
     4321 agcaggcgtc gtgcgtcacg cgcatcctcg tgcatcttct cgaccatggc gccgaggctc
     4381 gcgaagcgct cctcgtcgcg gattttcgcc acgaactcga cctcgatctc gcgaccgtac
     4441 aggtcagcgt cgaaatcgaa caggaaagtc tcgagccacg gctcgacacc gtccaccgtc
     4501 ggccgcgtgc cgaggctcgc gacaccgggc cacggattcg cggccacgcc gtgtacgcgc
     4561 accgcgaaga tcccgccgag cggcgcgcgc cgccgcttca gccgcaggtt cgcggtcgga
     4621 aagcccagcg tacgccccag ccgctcgccc cgcgaaacgc gcccacgcat cgtgtagggc
     4681 cgacccagca ggcgtgccgc ggcagcgaaa tcgccgctcg cgagaccggt gcgcacggcg
     4741 gtgctgctga tgcgctcgcc gctgcccacc ggcgcgacga cctccacctc gaagccgtgg
     4801 cgcctgccga gcgcctcgag cgtctgcgcc gtcgcggtgc cgccgcaccc gaagcggaaa
     4861 tcgtggccca ctaccacgac cgatgcggca caggtcgcgg cgagcgcgcc ggcgaagtcc
     4921 tcgccgctga gactacgcaa acgctcatcg aagcgcagta cgcacaggta ctggaggccg
     4981 ctgccttcca gaagtcgcca gcgctcacgg aaattcgtca gtcgcgccgg cggtgactgc
     5041 ggctgcagga attcgcgcgg cagcggctcg aaggtgagca tcatggcgcc tgtgccgcgc
     5101 acctcggcgt gccgccgcgc gcgttcgatc agctcgtggt ggccaaggtg caatccgtcg
     5161 aaagcgccca cggcgaccac cgggccccgc cggacagtgc gcaaaccggt ccaattgcga
     5221 atcaacttca tgattatatt ggccggttgc gcaagtcgcc atatcgcagg ccactgacga
     5281 aaagtaccgc gaagtacagc ccggcagccg ccgtgatgcc gatcgcacag cgcagcgccc
     5341 gctgcagcgc ccccatggca gtccacgtgt cgaggtcgcc ggtaaaccac cacagcagtg
     5401 ccgccatcgc ggcgttggca gtcagcacgc gggcgaggaa ccgaccccag cccgccgatg
     5461 cccgataaac gcccgcgcgg cgcaggcccc gccagagcag gaacgcgttg accgcggcgc
     5521 cggcgcaggt cgaggtcgcg agcagcacgt gcgggatcgg aaagccgagc cacttggccg
     5581 gcaataccac cacgacgttg agcgacatcg tgaccgcgaa cccgatgatc gccacgcgca
     5641 ccggcgtgcg cgtgtcctgg cgggcgtagt aacccggcgc cagcaccttg acgatgctga
     5701 acatcagcag cccccacgag tacgccatca gggcatacgc cgccatctgc acgtcgcgcg
     5761 agctgaactg gccgtaaccg aagacggtcg ccgtcagcgg gccggcgaag aacagcagtc
     5821 cgacggctgc gggtgcagcg agcagcacgg tgagtcgcag cgcccagtcg agggtgccgg
     5881 cgaagcgatc catcgaggcc tcggcgtgct gcgcggacag gcccggcagg atcacggtcg
     5941 caagggcgat gctgaacacg ccgagcggaa actccatcag ccggtcggcg aagtacagcc
     6001 aggaaatgct gcctgacatc aggaacgagg cgatgatcgt atcgagcagg aggctcacct
     6061 gcgccatcga cgagccgacg ataccgggca ccatcaaccg cccgatgcga cgcacaccct
     6121 cgccgcgcgg ctgccagcgt ggccatgcca gcagtccgag cttcgcgacg gccggcaact
     6181 ggaacagtac ctgcagcacg ccgctcacga agacaccgat cgcgagcacg atgcccggat
     6241 tggcggagtt cgcggcgagc accaccgcga agaaaatcat cacgacgttc atgatgacct
     6301 gggtgaacgc cggcaccgcg aatcggccgt agctgttcag cacgccgctg cagagcgcgg
     6361 tcagcgagac aaagaacagg tacgggaagg tccagcgcag catgtcgacg gcgaggtcgt
     6421 acttgtcgcc gtgctgggtg aagcccggcg cgaacagcag gatcaggacc ggcgcgccga
     6481 tcacgccgat gagcgtcacc acgaggagca ccgtgccgag ggtccccgcg gtgccgctga
     6541 ccaaatcccg gacctcgccc tgcgcgcgct tgacccggaa ctcggaaatg accggcacga
     6601 aggcctggga gaacgcccct tcggcaaaca ggcggcgcag gaaattcggg atcttgtagg
     6661 cgaccaggaa ggcgtccatc agcagcccgg cgccgaagac ctgcgacagc gccatgtccc
     6721 gcagcaggcc cgtgatccgc gatacgagcg tcgttgcgcc aacgacgccg gtgctcctga
     6781 ggaaggcgcg gctcatgcgg taagcgggtg aaaagtcacg ggttccgact ataccggacg
     6841 ccctcggcat cgcgtggccg cagattgaca agccgggggg cggtcagcac actacgcgcc
     6901 cccaaaaggc agtagatccg cagccggaga gaccgttcgt ggccaattcc aagtccgccg
     6961 aaagagccgc ccgcagagcc gaaaagcacc gcgcgcgcaa tgtcaccctg cgttcgagca
     7021 cgcgcaccac cctgcgcaag gtgaactcgg ccatcggcac cggcgacaag gcggcggcga
     7081 ccgcggccta ccgggatgcg gtatcggcgg tcgatgcgct cgccgcgaag ggcaagatcc
     7141 atcgcaacaa ggcggcccgc cacaagagcc gcctgtcggc gcgcgtcaaa tcgatgcagt
     7201 agtctcgggc cgggcggcct gcgcctcgat gtactgcatc acggcctcgg tcagctcttt
     7261 cgtcccctcg ccggtcacgg cggaaatcag gaacttcggc cccttgtagc gcagccgccg
     7321 cgcgatatcc gcggcgcgcg cctgcgcctc gtgggcgggc agcagatcgc gcttgttcag
     7381 cacgagccag cggggcttcg cggccagctc cggactgaac ttcttcagct ccgcaacgat
     7441 cgcgcgcgca tcgcgcaccg ggtctgcgtc gggatcgatc ggcgcaatat cgacgagatg
     7501 cagcagcacc cgcgtgcgct gcaggtgctt caggaaacgg atgccgaggc cggcaccttc
     7561 ggccgccccc tcgatcaggc ccgggatatc ggccatcacg aagctgcggt gctggcccac
     7621 gtagaccacg cccagattcg ggtacagcgt cgtgaacgga tagtcggcga tcttgggcct
     7681 ggccgccgac acggcgctga tgagtgtcga cttgccggca ttcggcaggc cgagcaggcc
     7741 tacgtccgca atcaccttca gttcgagctc gagcgtgcgc cgctccccgg gcaggccggg
     7801 accgaactgg cgcggggcgc ggttggtgct cgacttgaag cgcgtattgc cccagccgcc
     7861 cttgccacca cgggcgacca gcagttcctg gcccgggcgg gtgagatccc cgagcgtctc
     7921 ctgcgtttcc gcgtcgcgca cgatggtgcc gaccggcact acgacgcagg cgtccacgcc
     7981 gctgcggccg gtgcagccgt tgccgctgcc cggctggccg tgctcgccct tgaaagttcg
     8041 ctcgatgcgg aagtcggcga gcgtgttgat gccttcggca gcgcgcaaca ggacatcgcc
     8101 gccgttgccg ccgtcgccgc cgtcaggccc gccgaacggg acgaacttct cgcggcgaaa
     8161 gctcgtgctg ccgcggccgc cgttgccggc ctgcaccttg atgcgcgctt cgtcgacgaa
     8221 tttcatgctg acggaccccg gaggcgtgca tgcagtctac aaacaaaaac cccggcgcag
     8281 ggccggggtt ggcgtgcctt gaaagcggcc gaccttactc ggcgtcgacg ctcacgaagc
     8341 ggcgctgctc ggcgcccttg accgcaaacc tgaccgtgcc gttcttcagc gcgaacagcg
     8401 tgtggtcggt gccgatgccg acattcgggc cggcgtggta ttcggtgccg cgctggcgca
     8461 cgaggatgtt gccggcaagt accgactcac cgccgaactt cttcacgccg aggcgcttgc
     8521 tgtgggattc gcggccgttg cgggtgctgc cgcctgcttt cttgtgtgcc atgtctcaac
     8581 tgctccgtaa tctgtggctc gcgtgggcga tcagccgatc gccgtcaccc tgacctcggt
     8641 aaagggctgg cggtggttct tcatccgcag gtaatgcttg cggcgacgaa acttcacgat
     8701 cgccaccttg gccgccttgc cgtggcgctc gaccgtgccg gtcaccgtgg cgcccgacag
     8761 cagcggcgca ccgaccttga tgtcgctgcc gttaccgacc agcagcacct tgtcgaaggt
     8821 gaccgtggcg ccgggctcgg cctcgagctt ctcgatgcgc agcacctgac cctgttccac
     8881 ccgatactgc ttgccgcccg tggcgatgac cgcgtacatg atccaagact cctgaaaacc
     8941 gcttgcggac caagggcttg gcgcgggagg cgccgccggt ccaccatgca aagggcgcgt
     9001 agtgtactca gcgggctcga tacggtcaaa ctgttgatgt gcctatgaaa aagggcggtg
     9061 ctaaccgccc cctgcctccg ctagcatgcg ccatcccgac cggctgcaca tataaatgac
     9121 actcgacgaa atccgcgccc tcgcccaacc cgacctggct gccgtggacg atgtgatccg
     9181 ggccggattg catagcgaag tccccctcgc caaccagatc gccgagcaca tcattgccgg
     9241 tggcggcaag cgcttgcgac cgctgctggt ggtgctggcc ggccgcgccg caggcgctgc
     9301 cggcactcgc catatcgagg ccgccgcgtt catcgagttc atccacaccg cgacgctgct
     9361 gcacgatgac gtggtcgacg gatccgagct gcggcgcggc cgccacacgg ccaaccaggt
     9421 cttcggcaac caggcgagtg tgctcgtcgg agacttcgtc tacacgcgcg ccttccagat
     9481 gatggcggcg atcggctcgc agcgggtgat cgagatcatg gcgatggcga ccaatgtgat
     9541 cgccgagggc gaagtgctgc aactgatgaa cgcgcacgat gccgacacga ccgagcaacg
     9601 ctacctcgac gtcatctatc gcaagaccgc caaactgttc gaggcgggcg cggaggtcgc
     9661 cgcggtgctg agcaatgccc cggccacggt gcaggacgca ctgcggcgct acgggtgcca
     9721 cctcggcacg gcctaccagt tgatcgacga cgtgctcgac tatcgatcag acccggatgc
     9781 acgcggcaag aacctgggcg acgacctcgc cgagggcaag cccacgctgc cgctcatacg
     9841 tgcgctctcg gtcggcgacg aggtacagcg cgcgcagatc cgcgccgcga tcgagcacgg
     9901 gggtatcgag cacctcgatg cgatcattgc gacgatcgag gcgaccggcg ggctcgatta
     9961 cgcgcaagga ctcgcacgcc gcgaagccga ccaggcgatc gcagcgctgc aggatgttcc
    10021 accctcgccg taccgcgacg cgctggccgc gctcgcggcg ttcgccgtcg cgcaccagac
    10081 ctgagtcccg cgctcgcgcc ccgtggggcg accgcatgcg acgcgcaaac gcatgcgggg
    10141 cgccgaagcg ccccgcatgg tcccgtcaca gtcgggaacc gctcagttgc ccttggccgc
    10201 agcctccgcc gcttccttcg tgacgagcgg gtagtagacc gagaatggct cgtcgttcgg
    10261 gtcgagttcc acgaaccgct cgtcccaatg atcgatcgtc agcgcccaac gcgcccgctc
    10321 gccacggaca tagtcctcga tgtccgggta gagcttgcgg atcagttcgg cgcgtgacag
    10381 ctccttctcc ttgggcttcg ccaccacttc gcccggcttc ggcccgagac tgatgatcgc
    10441 gaagtcccag ggagcgcccg gggcatggtt gccggccgcg tagatcttcg acgtcccgcc
    10501 gctgtaccgg cggccgtatt ccacgatcgg cttgtggtac ttcttgtaga gtgccagcca
    10561 ttcgtcctgc ttgccgggcg tcaccttgta gtaggacacg ccgacggact gcttgccgac
    10621 gccgcggcca ttctggccct gggccagcgc agcgtccgtg ccgagcgcga acatgccggc
    10681 aatggcgacc gccagggctg cgaagcgggc ggagggtttc aacttcgtca ttatggttcc
    10741 tcgttgatgg ttcagaaagt gaagcgcacg gccaggtagt actggcggcc gtagatgtca
    10801 taggtgctcg actgggtgga accgagcgac gtgccgacga gcggcggctc cctgtcgtcc
    10861 acgttgttga caccaccgtg cacgcggacc agatccgaca cttgccagga tccgtacagg
    10921 tcgaagtagt tgaacgcgga cacgcccggc acggtcgagt tcggattcgt caccagcgac
    10981 gagtgccgca tggcgtccag gtagcgccag cgcaggccgg cagtgattgc gggcgtcgcg
    11041 tactggaagc gcgtgactgc cttccagtcc gggagcgaac cgggctgcgt cgcactcggc
    11101 gtgccgacgc tgccgccgta ctcgagcacg ttgctgccct tctgcagctg cacttcgaac
    11161 gagtcgagcc agctcgccac ggtgttcacc gagaacggtc cccaggccca gtcgacctgg
    11221 aagtcgacgc ccttcgtgcg gtagctgccg aggttgagcg tcggctggcc cacgttcgcg
    11281 atgaaacccg tcgccggatc gcgggtgatc tgcgagcaat agaaattact gttcgagagc
    11341 gccgggttcg agccgtcctg gttgaagcac ttcggcagga tctgggccgc gccaatcgtg
    11401 ccgatcacgt cctcgatctc gatgtcgtag tagtcggccg agacctgcag gcgcgcgagg
    11461 tactcgctct cgaacggcga ggtccacacg acaccgaaag tcctggtatc gcccgactcc
    11521 ggtcgcagcg aagtgttgcc gctgctggtg gcgagaatct cgtcctgcgt gttgacgaac
    11581 gtgtcgatga tgccgggagc gatgccctgc gcgaggcaca gtgcgcgcgc agcggcgggg
    11641 tccgcggccc gcgcctggct ctggtaatgg cagggatcgc cgccgcccga ggccacgttg
    11701 ccgatgccgg ggatcgacac gttcggcgcg acgaacagtt ccccgacgct cggcgcgcgc
    11761 acggcatgct ggtagccgcc gcgcaggcgc accgaatcga cgagctccca gctgaaatcc
    11821 gccttgtagg tatcgacgcc gccggcgaga tcgtaatccg agtagcggta ggcgacgttc
    11881 aggtcgagct gccgcgcgag cagccggtcg cgcagcagcg gcacgagcag ttcgccatag
    11941 acttcggcca cgtcggaccg accgcggctt tcgccggtgc ggaacacccc gaccacgttg
    12001 ccgagtgcaa ggtcgtggtc cggctggtag tcatagctct ggctacggaa gcccgcgccc
    12061 gcggcgaagc gcagatcccc cgccggcaac tccagcaccc tgccctgcag cgtggcttcg
    12121 atcacgttct gctcgaggtt cgtctcgctg atcggcgtgg cgaccaggaa gttgcggcac
    12181 tcgggcgaga gctgcgagaa gccgaacggg ttgtagccgc ccgcgcagag gctcgcgccg
    12241 ccgtcaggtg cgttcagcag cgccttgaga ttattgacga gcaccgatcc ggcgttcgtc
    12301 tcgatcttct gcgtgttgcc gcttgcggcg tagacgtccc agctgccgtc gatcgcctcg
    12361 atcggcccct tgaggccggc ggtcagctga tacacgtcga actggcgatt gaactcgcgg
    12421 gcgccggcct cggaatggaa gcgcttctcg aaacgaaacg gcgcatcggg atccggccgc
    12481 gaggcgagga tggtcgcgag gtccgccggg atgaacggat tcgtcaccgg cacgaggaag
    12541 gcatcgttgc ccgcttcggc gttggtcagc gactcatacg tcgtgtagta gacctgtgcg
    12601 taggcggtga gcttctccga caggtcatag ctgccgcggc caaaggccga atagcgcgtc
    12661 aggggcacct gcaggcgcga gaccaggttc aggttgttga gctgcgtgcc ggtgtcgaag
    12721 aggacgccgt cgggaccccg gtagttgaac ggaccgtgac tcgccgcgaa cagcgtgccg
    12781 tcgtcgttgt agccgagcac agcgacattg ggcacgcgac ccgccgcggc gccgtactgc
    12841 gcgaaaacct ggtcgacggc cgcctgggtc ggcaggttcg cgccgttcac attggtgcga
    12901 tagatgccgg tcgggatgcg gaagtcggtg ccgccccgcg cgcgggcgaa gaagtcgcgc
    12961 gacatgtaac cgacatcttc gcgatcgaga taggagaagg acacgaccgc attgccgcga
    13021 tcatcggcga agttgccgcc gaccgtgagc gaggtatcga gcgttgcgcc gtcgccctcg
    13081 ccgaacacgt tgtactgcgt atcgatctgc acgccttcga aacgcggatt gatctgcagg
    13141 ttgaccacac ccgcgatggc gtcggatccg tagacggctg acgcaccgcc ggtgatgacc
    13201 tcgacgttct ggaccagtgc cgatggaatc gtgttgatgt cgacgacacc gagcacgtcc
    13261 gccggctgca ggcggcgccc gtcgagcaga acgagattgc gggtcgggcc gaggccgcgc
    13321 aggttgagcg tcgcctgtcc ggtgccgctg aagccggcca cggtggcatt ggcaccgatg
    13381 gcgaactgcg gcagcgcgtt cagcgccact tcgagcgtcg gcgcaccact tgcctcgagc
    13441 gcctccatgg tgaccgtcgt cagcgggctg ttgcccacgt aatccttgcg gacgatgcgc
    13501 gatccggtga tggcgacttc ctgcaagggt tcgagtgaca cggagcctgc cggatccgcg
    13561 ctgtccgccg ccggcgcggt gacggcaact gcgtcggcgt cggcgagtgc ggcggccgaa
    13621 ccgaagacga gcgcggccgc gaccagcgat gcggcgcccg ggatggacct gttcaaagtt
    13681 gttccctgat cgagttgaag agtgacgaca gggaaaatag cgatgcacct cacagcaccg
    13741 ccaatcatca aatggaagtg gcttacgccg tgaggttata agagcgtgcg cgcatcgaac
    13801 tcccaggtct caccgccccg ggcgcccgcc acgcagcgca cccgcgccct cgaggccgca
    13861 atgaaccagg tctgctgctg cttcggcgcg ccgcgaccga agacaccggg cacgagcgcg
    13921 gccacgcagg cggcatgctc cgggtcgcgc accgactcat agcggatgag ttcgacctgc
    13981 gcctcacgcg ccgccgcggc cagcgcctgg gtgcgcgtgt aatccgaatg gtgggtccag
    14041 gcggcgcggt ccttcgatag cggcaggacc gtcaggtcga tcgacttgcc gcgcaccgaa
    14101 gcggcgaagg ccgtgtgcgt gaccggcttc aggtccggcg tcccggggga gtcgagcagg
    14161 aaacgcaggc gccaatagct cttctccgcg agcgcagtac gcagcgcttc cgccccgtac
    14221 cacacgccac cgtcgaacgg cgcccgaaag cgcgaaccgc tgcgcggccg gtagcgaaac
    14281 ggtgtggcca gcaggtagtg caacttcgcg gtgcccggcg gcagcggcgg cttgctggcc
    14341 tcgaggatgg attcgagcag tgcctgctcg gccagcgaat cgacgaggcg catcgtcgag
    14401 gcggtgtgct gcgcctcgac cagccgtttg gcgatggtct gacggcggc
//
LOCUS       DAOQVY010000054        14437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_4736_length_14437_cov_5.450424, whole genome shotgun sequence.
ACCESSION   DAOQVY010000054 DAOQVY010000000
VERSION     DAOQVY010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14437
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4736_length_14437_cov_5.450424"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..466)
                     /locus_tag="PKN91_06700"
     CDS             complement(101..466)
                     /locus_tag="PKN91_06700"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNS27797.1"
                     /translation="MDRRARGQDAEERAALFREAQGLTILARNFRRRAGEIDIVARDG
                     ATLAIIEVRSRATRSHGGAAASVGARKQQRLIRAAHLLLAAHPEWRRMPARFDVVIEE
                     GDGAAARIEWIRAAFALGG"
     gene            complement(480..2336)
                     /locus_tag="PKN91_06705"
     CDS             complement(480..2336)
                     /locus_tag="PKN91_06705"
                     /inference="COORDINATES: protein motif:HMM:NF016251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HNS27798.1"
                     /translation="MSTPLFSRALPFALLLATALTACTSIGPASGPVPTLERAEQSAQ
                     QGRHAEAARIYEALAAERSGAAAIDMQLRAARAWLDARQPDAAGRALAAARVGASPAQ
                     AFEAQMLGVELRIARGDAQRAWSEVSAMAQPAARADAARYLDVRQQAAFATGRHAEGV
                     RAQIAREPLLPTLEAQRRSRSELLADLRAALEGGAKVNPAAEADPTVRGWLELAPIAV
                     TAATMPTTVGPALQHWRGRYPSHPANDVVRSDIEMIEAPQARDRASHVALLLPVSGRQ
                     AGAAAQIRDGFFTAYYRSPAGTRPQVRVYDTASTSIAEAISRASAAGATVIVGPLLRD
                     EVAAAAGYIGPRPALLALNFLGADDITPANFYQYALSPENEARSIAGRLTSQGLRRGV
                     ALVPVGEWGTRVLAAFTEALQAAGGELVGTATYFPAENDYSPAITDLLRIDASKARHR
                     RIEQVTGTKLAFQPRRRGDAQFIFAPSQAQTARLLRPQLRFHYAGDLPTYSTSDAYEP
                     HPTANQDLDGLIFPDMPWVFGVGAKSAEVRESLVGAWGESGAARGKLFAFGHDAYQLS
                     IAIASGAPVDIDGLTGRLRIDDGRRVYRQLEWAQIKGGQTMLLDAGMPAVTP"
     gene            2367..3170
                     /gene="rsmI"
                     /locus_tag="PKN91_06710"
                     /pseudo
     CDS             2367..3170
                     /gene="rsmI"
                     /locus_tag="PKN91_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
     gene            3263..3621
                     /gene="rnpB"
                     /locus_tag="PKN91_06715"
     ncRNA           3263..3621
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKN91_06715"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            3832..4284
                     /gene="mraZ"
                     /locus_tag="PKN91_06720"
     CDS             3832..4284
                     /gene="mraZ"
                     /locus_tag="PKN91_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNS27799.1"
                     /translation="MFRGATKVTIDDKGRMALPTRYRAAVAERCEGRMVVTVDRDQCL
                     LIYPLPDWEQIERKLMSLPSLHPQARRLQRLMVGHATDLELDGHGRLLLSPELREFAS
                     LSRLAMLVGQGNRFELWDEARWNERRDAWLAHEEAVTDLPAELDSLSL"
     gene            4344..5267
                     /gene="rsmH"
                     /locus_tag="PKN91_06725"
     CDS             4344..5267
                     /gene="rsmH"
                     /locus_tag="PKN91_06725"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNS27800.1"
                     /translation="MDEHVPVLVEEVLEGLAPKADGLYIDATFGRGGHTARILEALGG
                     EGRVLALDRDPQAIAAGRVRFATEMRLTLVQAPFADLRQVVHEHARGRPVAGVLLDLG
                     VSSPQLDEAGRGFSFSKDGPLDMRMDPSCGEPVAAWLARADVDEMREVIATLGEERYA
                     RRVAQAIAAARTRAPIERTGQLAQVIAGAVRTRDGKHPATRTFQALRMHVNDELGQLE
                     RGLAAALDVLAPGGRLAVIAFHSLEDRMVKQFMQRESSVDPVLAKLPVLPPHAAPRLR
                     LVGGKRRASEAEIARNPRARSAVLRVAERAA"
     gene            5267..5548
                     /gene="ftsL"
                     /locus_tag="PKN91_06730"
     CDS             5267..5548
                     /gene="ftsL"
                     /locus_tag="PKN91_06730"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNS27801.1"
                     /translation="MALSRRTLLVAIPLLWVLALGSAAGAIWAKHHSRELFVELERLN
                     AQRDDLEVEWGQLQLEQSAWSTHAFVENVARARLRMAMPPPQEIEVVRP"
     gene            5545..7320
                     /locus_tag="PKN91_06735"
     CDS             5545..7320
                     /locus_tag="PKN91_06735"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNS27802.1"
                     /translation="MRERRGKGGGGRYGGGESAPRSFRTRAWVVLGMLVVAAGGLLWR
                     AVDLQLMDHAFLARQGDARFSRVAAIAAHRGTITDRYGEPLAVSTPVDSVWANPKELA
                     LATDLLPQLARALKVDKNDLARRVTSNLDREFLYLRRHMQPADAQKIKALGIPGVYLV
                     REYRRYYPSGEVVGHLLGFTSIDDAGQEGIELAFDHWLAGEDGAKRVIQDRHGRVVQD
                     VESIRPVRPGRDMVLSIDLRIQYLAYRELKAAIREQRAKSGSVIVLDVTTGEVLAMVN
                     QPAYNPNDRDQINARTYRNRAATDILEPGSSIKPFVIAAALASGKYSEQSIVDTSPGY
                     IKVGARMLEDKHNLGRIDLATVLAKSSNVGMTRVALELEPEQMWNTLTGLGFGQVTTS
                     GYPGESAGLLPHWSHWRPVGISSMSRGYGLSVTPLQLAHAYATVGAGGHSRPVSFLRV
                     DAAPIGERVLDERVARSLVHMLEAVIAPSGTGSHAAIPGYRVAGKTGTAWKANAGGYS
                     TDRYMAVFGGVAPASQPRLAAVVIVDEPGAGKYYGGDVAAPVFSRVVGGALRLLAVAP
                     DAAIGAPGDDDAGTPAPSRRQVALR"
     gene            7332..8810
                     /locus_tag="PKN91_06740"
     CDS             7332..8810
                     /locus_tag="PKN91_06740"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNS27803.1"
                     /translation="MNVPRRLADLVGDLVKVPARLEVTDLSVASGEVRAGGLFFACAG
                     RTTHGLAHVHEAIARGARVVLWEPAPDLRVPDFPSGIFAAPVPQLHAQLGAIADRFFG
                     EPSATLTTVGITGTNGKTTTAWLAAEALAASGRRATYSGTLGYGTPGALRTSSYTTPD
                     AIEVHRRLAALRDEGADCVAMEVSSHALDQGRVAGVRFHTAVFTNLTRDHLDYHGTMA
                     EYGAAKAMLLSWPTLTTRIINIDDPFGSALARAHAGDSGRLVTVSRRDHANVRASAVR
                     AATCGVEIDVATDWGAGTLATRLIGDFNADNALAVLALLLAWDVPFATVLRAIGQCTA
                     PPGRMESFGGTAGAPLVIVDYAHTPDALDKALAAARAHTDGKLTVVFGCGGDRDPGKR
                     PLMGGIAARRADRIWLTDDNPRTESPADIVAQTARGIAPGAALRIEHERAAAIRGALD
                     EAGAGDVVLVAGKGHEDYQIVGHEHRVFSDQAVVRDVLGSRK"
     gene            8807..10174
                     /gene="murF"
                     /locus_tag="PKN91_06745"
     CDS             8807..10174
                     /gene="murF"
                     /locus_tag="PKN91_06745"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNS27804.1"
                     /translation="MSGPQRTLARLAAACGGQLVGADRAYSGVSTDTRTLQVGQVFFA
                     LQGPRFNAAEFAGAAAAGGAAAVVVPARQDVAIAQIIVDDTLAALTRAAAAWRAHFAI
                     PLIGVAGSNGKTTAKGMTASILGQAGTCLATLGNLNNHIGVPLTLLRLESTDEFAVIE
                     IGANRPGEVAALAALAKPGIGLVTNAGAEHLEGFGSLEGVARAEGELFEALPADGVAV
                     INADDVFSEMWRGATRARVVTFGLTNAADFHARDITTEVGSGGFSTAFTLHSPVGDIA
                     IRLQLAGLHNISNALAAAAAAVSAGATLAQVAAGLAAMQPVKGRLNLRVTRAGAWLID
                     DSYNANPSSMRAGIDVLVGLGGRRWLAIGDMGELGTHADEAHADIGRYARARGVERLF
                     ATGPLSALAVEAFGAGAQWFPDAETLTRELESALDADVRLLVKGSRSNRLERVVDALV
                     GDRAG"
     gene            10179..11264
                     /gene="mraY"
                     /locus_tag="PKN91_06750"
     CDS             10179..11264
                     /gene="mraY"
                     /locus_tag="PKN91_06750"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005297383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNS27805.1"
                     /translation="MLYWLARELTAEYSGFNVFSYLTLRAILATMSALAISLLVGPAM
                     IRRLSRYQVGQVVRQEGPQSHLPKAGTPTMGGALIIVAILVATLLWADLSNRFVWIVM
                     AVTLAFGAIGFYDDYLKLVVGDSTGLAPRWKYFWQSVVGLIVAAVLWYTARTPAETTF
                     YLPIVKSVALPMAAAGYIILSYLMIVGMSNAVNLTDGLDGLAIMPAAMVAAALGVFAY
                     ASGNAVFANYLGIPAVPQAGEVLIFCAAMVGAGLGFLWFNSYPAQVFMGDIGALALGA
                     ALGVIAVIVRQEVVALVMGGVFVLETASVILQVASFKLTGKRLFRMSPIHHHYELKGW
                     AEPKVIVRFWILSLLLVLAGLATLKLR"
     gene            11261..12625
                     /gene="murD"
                     /locus_tag="PKN91_06755"
     CDS             11261..12625
                     /gene="murD"
                     /locus_tag="PKN91_06755"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNS27806.1"
                     /translation="MSTATTGARYAVIAGLGRTGLSCARYLHAEGWRIAVTDTRPRPP
                     ELDALRALVGDIVVRAGGLDTGLLEGATLVVASPGLALTDPFFVTARARGLEVVGDIE
                     LFARAARAPAVGITGTNGKSTVTTLVARMARRAGLTVRVGGNLGEPALDLLGDPATAL
                     YVLELSSFQLETTRTLELAAATVLNVTADHMDRYATLADYAAAKARIYARCHAAVINL
                     DDPLVAAMRAPVARDLPRTTLTFSLRAAIGADYALADRGAEEGWWLTRRGEPLMALAE
                     LRIPGLHNAANALAALALGEALALPLAAMLAELREFRGLEHRTQWVAEVGGVRYVNDS
                     KGTNVGATLAAVSGLPGTLVIIAGGDGKGQDFGPLAAAFRDKVRHVVLIGRDAPAIAA
                     VLEGVCPLSRAETLEAAVRTAASVARAGDTVLLSPACASLDMFRDYTQRGRVFEQAVK
                     ELAA"
     gene            12639..13814
                     /gene="ftsW"
                     /locus_tag="PKN91_06760"
     CDS             12639..13814
                     /gene="ftsW"
                     /locus_tag="PKN91_06760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNS27807.1"
                     /translation="MNAATLGWARSGSRGGAFRTDWVTVALVLAITLLGLVMVTSASV
                     SIANNETGQPFYYLERQLALLLAGGVAAVVLTRVRTELLEKLALPLLIVAIALLVAVL
                     IPGLGHVVNGSRRWIRLLGFNFQASELARLLVLIYVASYAARRESELRTSFSGLARPL
                     GLLALVGVLLLAEPDFGAATVLFATGFGVLFMAGARLRHVLAMVLAAVAAFGALVALS
                     GYRMRRLATFLDPWADPYDSGFQLTQSLIAIGRGEWFGVGLGESVQKLFYLPEAHTDF
                     LFAVLAEELGLVGVVLTVGLFIALAWRALHIAQLASDAGLRFQACLAAGFGLWIGMQA
                     FINIGVNMGALPTKGLTLPLMSYGRSSLIVTIAWVGILLRVHHEAASRTRGPATVSA"
     gene            13811..>14437
                     /locus_tag="PKN91_06765"
     CDS             13811..>14437
                     /locus_tag="PKN91_06765"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HNS27808.1"
                     /translation="MSGGTVIIMAGGTGGHVFPALALARILQARGHDIVWLGTREGIE
                     ARLVPAEGLPIEWISIGGLRGKGMGTLLAAPFRLARALYQSLRAMRRHRPIVVVGLGG
                     YVTGPGGLAAWLARRPLVVHEQNAIAGYTNRVLARIARDVLAAFPDAFDGAVPVSVIG
                     NPVRREFFELGEPAARAAGRGAVLRLLVVGGSQGANKLNAVVPFAIARL"
BASE COUNT         1975 a         5032 c         5194 g         2236 t
ORIGIN      
        1 cgggtgtcca aattgcctca tgttgaatgc catataccag aattcgccat gcaccggtgg
       61 cccgcggtgg cgcgcggcac ccgggccgca ccgcaaggca tcaacccccc agcgcgaacg
      121 cggcgcggat ccactcgatg cgcgccgctg caccgtcccc ctcttcaatc accacgtcga
      181 agcgcgccgg catccgtcgc cactcgggat gcgcggcgag cagcaggtgg gcggcgcgga
      241 tcaggcgctg ctgcttgcgc gcgccgacgc tcgccgcggc gccgccgtgg ctgcgggtcg
      301 cgcgcgagcg cacttcgatg atcgcgagtg tggcgccgtc gcgcgcgacg atgtcgatct
      361 cgccggcgcg gcggcggaag ttgcgcgcga gaatcgtcag gccctgcgcc tcgcggaaga
      421 gcgccgcgcg ctcttcggca tcctggccac gcgcgcgacg atccatggcg gagtgcggct
      481 cagggcgtga cggccggcat gccggcgtcg aggagcatcg tctgcccgcc cttgatctgc
      541 gcccattcga gctggcggta cacgcgccgg ccgtcgtcga tgcgcagccg gccggtgagg
      601 ccgtcgatgt cgacgggcgc gccgctcgct atcgcgatgc tcagctggta ggcgtcatgg
      661 ccgaatgcga acagcttgcc gcgcgctgcg ccgctctcgc cccaggcgcc caccagcgat
      721 tcgcgcacct cggcgctctt tgcgccgacg ccaaacaccc agggcatgtc ggggaagatg
      781 agcccgtcga ggtcctggtt ggcggtcggg tgcggctcgt acgcatcgga ggtcgaatag
      841 gtcggcagat cgccggcata gtggaagcgc agctgcgggc gcagcaggcg cgcggtctgc
      901 gcctggctcg gtgcgaagat gaactgcgca tcgccgcggc ggcgcggctg gaacgcgagc
      961 ttcgtgccgg tgacctgttc gatgcggcga tgccgcgcct tgctcgcgtc gatgcgcagc
     1021 aggtcggtga tggcgggcga gtagtcgttc tcggccggga agtaggtcgc ggtgccgacc
     1081 agctcgccgc cggcggcctg cagcgcctcg gtaaaggccg caagcacgcg ggtgccccat
     1141 tcgcccacgg gcacgagcgc gacgccgcgg cgcaggccct gcgaagtgag ccggccggcg
     1201 atcgagcgcg cctcgttttc cggcgacagc gcgtactggt agaaattggc gggggtgatg
     1261 tcgtcggcgc cgaggaaatt gagtgcgagc agtgccggcc gcgggccgat gtagcccgcc
     1321 gccgcggcca cttcgtcgcg cagcagcggg ccgacgatca cggtagcgcc cgccgcgctc
     1381 gcgcgcgaaa tggcttcggc gatgctggtg ctcgccgtat cgtagacgcg cacctgcggg
     1441 cgcgtgcctg ccggtgaacg gtagtacgca gtgaagaagc catcgcggat ctgcgcagcg
     1501 gcgccggcct ggcggcccga tacaggcaac agcagggcga cgtgcgaggc gcgatcgcgc
     1561 gcctgcggcg cctcgatcat ttcgatgtcg ctgcgcacga cgtcgttcgc cggatgcgag
     1621 ggatagcggc cgcgccagtg ctgcagcgcc gggccgaccg tcgtcggcat cgtcgctgcg
     1681 gtgaccgcaa tcggcgcaag ctccagccag ccgcgcacgg tggggtcggc ctcggcggcg
     1741 ggattgacct tcgcgccgcc ctcgagcgcc gcgcgcaggt cggcgagcaa ctcgctgcga
     1801 ctccggcgtt gtgcttccag cgtgggcagc aacggttcgc gcgcgatctg cgcgcgcacg
     1861 ccttcggcat ggcgcccggt ggcgaacgcc gcctgctggc gtacgtcgag atagcgcgca
     1921 gcgtcggcac gcgcggctgg ctgcgccatc gcgctcactt cgctccacgc gcgctgcgcg
     1981 tccccgcggg cgatgcgcag ctcgacgccg agcatctgcg cctcgaaggc ctgcgcgggg
     2041 ctcgcgccca cgcgcgccgc ggcaagcgcg cgcccggcag catccggctg gcgcgcatcg
     2101 agccaggcgc gggccgcgcg cagctgcata tcgatcgccg cggcaccgct gcgctcggcc
     2161 gcgagcgctt catagattcg cgccgcctcc gcatgccggc cctgctgcgc actctgctcg
     2221 gcgcgctcga gcgtcggcac gggtcccgag gccgggccga tgctggtaca tgcggtgagc
     2281 gccgtggcga gcagcaatgc gaacggcagc gcccggctga ataacggggt cgacatgtct
     2341 tgtttcacct tacactcggc ggcacgatgc ccgggtggcg cgatgaggag attatagtgg
     2401 gtgcgaacgc aggccggatc gatgtcatcg ccacgcccat cggcaacctc ggtgatctct
     2461 cgccgcgcgc ccgcgatgcg ctcgcgcagg ccgacctcgt cgccgcggag gacacgcgcc
     2521 gcacgggcca gctgctcgcg gcgatgggcc tgtcgcggcc actcgtctcg ctgcacgagc
     2581 acaacgaggg cgaacgcatc gccgagctgc tcgctcggct cgacgccggc gccgtgattg
     2641 cgctggtcag cgatgccggc acgccgctgc tgtcggatcc cggctacgaa ctcgtgcgtg
     2701 cggcgcgcgc tgcaggccac acgttgcgca cggtgccggg cccgtcggca atcaccgcgg
     2761 cgctcagcat cgcggggatt cccaccgcgc gcttctgctt cgagggtttc ctgccggcgc
     2821 gcagccgcga acgccgcgag cgtctcgccc agctcgcgct cgagccgcgc acgctcgtgt
     2881 ttttcgaggc accgcaccgc atcgaggaga gcctcgccga tatcaccgcg gcattcggcg
     2941 ccgggcgcga ggcggctctc ggtcgcgagc tcaccaaagc cttcgaaacc ctgtatcacg
     3001 gcacgctcgg cgaactgtcg cagcgtgcac gcgaggacgc caatgtcgcg cgcggcgaga
     3061 tcacgttggt cgtggcgggt gcgccggacg tggcggtgag cggcgctgtc acgccggcgg
     3121 aactcgcccg catcgtcgcg ctgctcgcga aggagttgcc gccgagcaag gcggcggcgc
     3181 tggccgcgca gatcaccggc gcgcgccgca gcgaggccta cgatatcgcc atcaagctcg
     3241 gcgggggtgt atcctcccgc gcggagtcgg ccgggtaatc gctgtatctg cctcggcagg
     3301 tatggaggaa agtccgggct cctgcggacg aggtgccagg taacgcctgg ggggcgcgag
     3361 cccacggaaa gtgcaacaga aagcaaaccg ccccttcggg ggtaagggtg aaagggtgcg
     3421 gtaagagcgc accgcgtcgg tggtaacact cgacggcacg gcaaacccca cctggagcaa
     3481 ggccaaatag ggaagcggcg ggtcacaccg cagcgcatgt ccgcgcgtgc ttccgggtag
     3541 gccgctcgag gcccccggtg acgggggtcc cagaggaatg attgccctcg acagaacccg
     3601 gcttacaggc cgactccttc tcaactactg tataagcttc gatctacaag cctctgatag
     3661 ctaagaggtc ctccccgcgg ccactgcgcc cgggcgggtc ggaacgaacg taagttcttg
     3721 tcgtcccgtc attttttccc catctgcgcg ttgacagtgc gccggcgcgt ttctatagtg
     3781 gcgccgagtg ggagaaagtg gggagaaatg ggctccacag gggtccaggc catgttccgc
     3841 ggtgcaacaa aagtcacgat cgacgacaag ggccgcatgg ccctgccgac ccggtatcgg
     3901 gcggccgtgg ccgagcgctg cgagggccgt atggtcgtca cggtcgaccg cgaccagtgc
     3961 ctcctcatct atccgctgcc tgactgggag cagatcgaac gcaagctgat gagcctgccg
     4021 agcctgcatc cgcaggcccg gcgactgcag cgtctgatgg tgggccatgc caccgacctc
     4081 gagctcgacg gacacgggcg gctcctgctg tcgcccgagc tgcgcgagtt cgcgtcgcta
     4141 tcacggctcg cgatgctggt gggccagggt aatcgcttcg agttgtggga cgaggcgcgg
     4201 tggaacgagc ggcgtgatgc atggctcgcg cacgaggagg cggtgacgga cctgcctgcc
     4261 gagctcgatt cactgtcgct ctgagtaacg gtttttctgg cgggggaggg ggcggtcgga
     4321 ccgcttcacg agggcgaggc gcgatggatg agcacgttcc tgtgcttgtc gaggaagtgc
     4381 tcgaaggcct cgctccgaaa gcggacggtc tatacatcga tgcgacgttc gggcgcggcg
     4441 gacacacggc acggatactc gaagccctgg gtggagaagg acgcgtgctc gcgctcgacc
     4501 gcgatccgca ggccatcgcg gccggtcgcg tgcgctttgc gaccgaaatg cggcttacgc
     4561 tcgtccaggc gccgtttgcc gatcttcggc aggtcgtgca cgagcacgcg cgcggccggc
     4621 cggtcgctgg cgtgctgctc gacctcggcg tgtcgtcgcc tcagctcgac gaggccggtc
     4681 gcggtttcag tttctcgaag gacggtccgc tcgacatgcg catggatccg tcatgcggtg
     4741 aacctgtcgc ggcgtggctc gcgcgcgcgg atgtcgacga aatgcgcgaa gtgatcgcga
     4801 cactcggcga ggaacgctac gcgcgccgtg tcgcccaggc gatcgccgcg gcgcgcacgc
     4861 gcgcgcccat cgagcgtacg ggccagctcg cccaggtgat cgccggcgcg gtgcgcacgc
     4921 gcgacggcaa gcacccggcc acccgtactt tccaggcgct gcgtatgcac gtgaacgacg
     4981 aactcggcca gctcgagcgc gggctcgccg ccgcgctcga tgtgctcgcg cccggcgggc
     5041 gcctcgccgt gatcgccttc cactcgctcg aagaccgcat ggtcaagcag ttcatgcagc
     5101 gcgagtcgag cgtcgatccg gtgctcgcga agctgccggt cctgccgccg cacgcggcgc
     5161 cgcgactgcg gctcgtcggc ggcaagcgcc gcgcgagcga ggccgagatc gcccgcaatc
     5221 cgcgcgctcg cagcgcggtt ctgcgcgtcg cggagcgcgc cgcgtgatgg cgctctcgcg
     5281 caggacattg ctcgtcgcga ttccgctgct gtgggtgctt gcgctcggct cggcggccgg
     5341 agcgatctgg gcgaagcacc actcgcgcga gctgttcgtc gaactcgaac ggctgaacgc
     5401 gcagcgcgac gacctcgagg tcgagtgggg ccagctgcag ctcgagcaga gcgcctggtc
     5461 gacgcacgca ttcgtcgaga acgtagcgcg cgcgcggctc aggatggcga tgccgccgcc
     5521 gcaggaaatc gaggtcgtgc ggccatgagg gagcggcgcg gcaagggcgg cggcgggcgc
     5581 tacggcggcg gcgaatcggc accgcggtcg tttcgcacgc gcgcctgggt ggtgctcggc
     5641 atgctggtcg tggcggccgg cgggctgctg tggcgcgcgg tcgacctgca gctgatggac
     5701 catgcattcc tcgcgcgcca gggcgatgca cgcttctcgc gcgtcgccgc gattgccgcg
     5761 catcgcggca cgatcaccga tcgctacggt gaaccgctgg cggtcagcac gccggtcgac
     5821 tcggtatggg ccaacccgaa agagcttgcg ctcgcgaccg acctgctgcc gcagctcgcc
     5881 agggcgctga aggtcgacaa gaacgatctc gcgcgtcgcg tcacgtccaa cctggatcgc
     5941 gaattcctct acctgcgccg gcacatgcag ccggccgacg cgcagaagat aaaagcgctc
     6001 ggcatccccg gcgtctacct ggtgcgcgag taccggcgtt actacccctc gggcgaggtc
     6061 gtcgggcatc tgctcggctt caccagcatc gacgatgccg gccaggaagg catcgagctc
     6121 gcgttcgacc actggctcgc cggcgaggat ggcgccaagc gcgtgatcca ggaccgccat
     6181 ggccgcgtgg tgcaggatgt cgagagtatc cggcccgtgc ggccggggcg cgacatggtg
     6241 ctgtcgatcg accttcgcat ccagtacctc gcctatcgcg agctgaaggc ggcgatccgc
     6301 gagcagcgcg ccaagtcggg ctccgtgatc gtgctcgacg tgacgacggg ggaagtgctg
     6361 gcgatggtga accagccggc ctacaacccg aatgaccgcg accagatcaa cgcgcgcacc
     6421 taccgcaacc gtgcggccac cgacatcctc gagcccggct cgtcgatcaa gccgttcgtg
     6481 atcgccgcgg cgctcgcgtc gggcaagtac agcgagcaga gcatcgtcga cacctcgccc
     6541 ggctacatca aggtgggcgc gcgcatgctc gaggacaagc acaacctcgg tcgcatcgac
     6601 ctcgccacgg tgctcgccaa gagctcgaac gtcggcatga cgcgcgtcgc gctggagctc
     6661 gagcccgagc agatgtggaa cacgctcacc ggcctcggct tcggccaggt gacgacgagc
     6721 ggttacccgg gcgagtcggc cggcctgctg ccgcactggt cgcactggcg gccggtcggc
     6781 atttcctcga tgtcgcgcgg ctacggcctg tcggtcacgc cgctgcagct tgcgcatgcg
     6841 tacgccacgg tcggcgccgg cggccattcg cgcccggtgt ccttcctgcg cgtcgatgcg
     6901 gcgccgatcg gagagcgcgt gctcgatgag cgcgtcgcgc gctcgctggt gcacatgctg
     6961 gaggccgtca tcgcgccgtc cggtaccggc agccacgccg cgatcccggg ctatcgcgtc
     7021 gccggcaaga ccggcacggc gtggaaggcg aacgccggcg gctattccac cgatcgctac
     7081 atggcggtgt tcggcggtgt cgcgcccgcc tcgcagccgc gcctcgcggc cgtcgtgatc
     7141 gtcgatgagc cgggtgcggg caagtactac ggcggcgatg tcgccgcacc ggtgttctcg
     7201 cgcgtggtgg gcggtgcgct gcgcctgctg gccgtcgcgc ccgacgcggc gatcggcgca
     7261 cccggcgacg acgatgccgg cacgcccgcg ccttcgcgcc gccaggtggc actgcgatga
     7321 tgcgcacgcc ggtgaacgtc ccgcgccgac tcgccgatct cgtgggcgac ctggtcaagg
     7381 tgccggcgag gctcgaagtc accgacctgt cggtggccag cggcgaggtg cgcgcgggcg
     7441 ggctcttctt cgcctgcgcc ggacgcacga cccacggcct cgcccatgtg cacgaggcga
     7501 tcgcgcgtgg cgcgcgcgtg gtcctgtggg agcctgcgcc cgacctgcgc gtgccggatt
     7561 tcccgtcggg gatcttcgcg gcgcccgtgc cgcagctgca tgcgcagctc ggcgcgatcg
     7621 ccgaccgctt cttcggcgag ccctcggcga cgctgactac ggtgggcatc accggcacga
     7681 acggcaagac gaccaccgca tggctcgccg cagaggcgct cgccgcgagc ggtcggcgtg
     7741 ccacctacag cggcacgctc ggctatggca cacccggcgc cctgcgcacg agttcgtaca
     7801 cgacaccgga cgccatcgaa gtgcatcggc ggctcgcggc actgcgcgac gagggcgccg
     7861 actgtgtggc gatggaggtt tcctcgcacg cgctcgacca ggggcgtgtc gcgggcgtgc
     7921 gcttccatac cgcagtgttc accaacctga cacgcgacca tctcgactac cacggcacga
     7981 tggccgagta cggcgctgcc aaagcgatgc tgctctcctg gccgacgctc accacgcgca
     8041 tcatcaacat cgacgatccg ttcggcagcg cgctcgccag ggcgcacgcc ggcgacagcg
     8101 gtcggctggt cacggtgtcc cgccgcgacc acgcgaacgt gcgcgcgagc gccgtgcgcg
     8161 ccgcgacgtg cggtgtcgag atcgacgtcg ccacggactg gggcgccggc acgctcgcga
     8221 cccggctgat cggcgatttc aacgccgaca atgcgctcgc cgtgctcgcg ctgctgctcg
     8281 cgtgggatgt gccgttcgcc acggtgctgc gcgccatcgg ccaatgcacg gcgccgccgg
     8341 ggcgcatgga gtccttcggc gggacggcgg gtgcgccact cgtgatcgtc gactacgccc
     8401 acacacctga tgcgctcgac aaggcgctcg cggccgcccg cgcgcacacg gacggcaagc
     8461 tcaccgtggt gttcggctgc ggtggcgatc gcgatccggg caagcggccg ctgatgggcg
     8521 gcatcgccgc gcggcgcgcc gaccgcatct ggctgactga cgacaacccg cgcaccgaat
     8581 cgccggccga catcgtggcg cagaccgcgc gcggcatcgc gccaggcgcg gcgctgcgca
     8641 tcgaacacga gcgcgccgcg gccattcgcg gtgcgctcga cgaggcaggc gccggcgatg
     8701 tggtgctggt cgccggcaag ggacacgagg actaccagat cgtcggccat gaacaccggg
     8761 tgttcagcga ccaggcggtg gtgagggacg tgctcgggag ccgcaagtga gtggtccgca
     8821 gcgcacgctc gcccgcctcg ccgcggcctg cggcggacag ctcgtcggcg ccgatcgcgc
     8881 ctacagcggc gtgtcgaccg atacgcgcac gctgcaggtc ggccaggtgt tcttcgcact
     8941 ccagggcccg cgcttcaatg ccgccgagtt cgcaggcgcc gcggccgccg ggggcgccgc
     9001 ggccgtggtc gtgccggcac gccaggacgt ggcgatcgcg cagatcatcg tcgacgacac
     9061 actcgctgcg ctgacgcgtg ctgcggcggc gtggcgcgcg catttcgcga tcccgctgat
     9121 cggcgtcgcg ggcagcaacg gcaagaccac ggccaagggc atgacggcgt cgatcctggg
     9181 ccaggccggc acctgccttg ccacgctcgg caacctgaac aaccacatcg gcgtgccgct
     9241 gacgctgttg cgcctcgagt cgacggacga gttcgccgtc atcgagatcg gcgcaaaccg
     9301 gcccggtgaa gtcgcggcac tcgcggcgct ggcgaagccg ggcatcggcc tcgtgacgaa
     9361 cgccggcgcc gagcacctcg agggcttcgg ctcgctcgaa ggcgtggcgc gggccgaggg
     9421 cgagctgttc gaggcgctgc cggcggacgg cgtggcggtg atcaacgccg atgatgtctt
     9481 cagcgagatg tggcgcggcg cgacccgcgc ccgcgtcgtg accttcggcc tgacgaatgc
     9541 cgccgatttc cacgcgcgcg atatcacgac cgaggtcggc agcggcggct tcagcaccgc
     9601 tttcacgctg cactcgccgg tcggcgatat cgcgatccgc ctgcagctcg cgggcctgca
     9661 caacatctcc aacgcactcg ccgcggccgc ggcggccgtg tctgccggcg cgacgcttgc
     9721 gcaggtcgcc gcgggactcg ccgcgatgca gccggtcaag gggcggctca atctgcgtgt
     9781 cacgcgcgcc ggcgcctggc tgatcgacga ctcgtacaac gccaacccga gctccatgcg
     9841 cgccggcatc gacgtgctcg tcggtctcgg cggccgtcgc tggctcgcga tcggcgacat
     9901 gggcgaactc ggcacgcacg cggacgaggc acacgccgac atcgggcgct atgcgcgcgc
     9961 gcgcggcgtc gagcggctgt tcgcgaccgg cccgctctcg gcgctcgcgg tcgaggcctt
    10021 cggggccgga gcgcaatggt ttccggatgc cgagacgctc acccgcgagc tcgagtccgc
    10081 gctcgacgcc gatgtgcgtc tgctggtgaa gggctcgcgg tcgaaccggc tcgagcgcgt
    10141 ggtcgatgcg ctcgttggcg acagggcggg gtgacggaat gctgtactgg ctcgccaggg
    10201 aactgaccgc cgaatactcg ggattcaacg tcttctcgta cctgacgctg cgcgcgatcc
    10261 tcgcgacgat gtcggcgctc gcgatctcgc tgctggtcgg cccggcgatg atccggcggc
    10321 tgtcgcgcta ccaggtcggc caggtggtac gccaggaggg cccgcagagc cacctgccga
    10381 aggccggtac gccgacgatg ggcggggcgc tgatcatcgt cgcgatcctc gtcgcgacgc
    10441 tcctgtgggc ggacctctcg aaccgtttcg tgtggatcgt catggcggtc acgctcgcct
    10501 tcggtgcgat cggcttctac gacgactacc tgaagctcgt ggtcggcgac tcgacggggc
    10561 tcgcgccgcg ctggaagtat ttctggcagt cggtcgtcgg gctgatcgtc gccgccgtgc
    10621 tctggtacac cgcgcgcacg ccggccgaga cgacgttcta cctgccgatc gtcaagagcg
    10681 tggcgctgcc gatggcggcg gccggctaca tcatcctcag ctacctgatg atcgtcggca
    10741 tgagcaacgc ggtgaacctc accgacgggc tcgatggcct cgcgatcatg ccggcggcca
    10801 tggtcgccgc ggcgctcggc gtgttcgcct acgcctccgg caacgcggtg ttcgccaact
    10861 atctcggcat cccggcggtg ccgcaggcgg gcgaggtgct gatcttctgc gccgcgatgg
    10921 tcggcgcggg cctcgggttc ctgtggttca actcctatcc ggcgcaggta ttcatgggcg
    10981 acatcggtgc gctcgccctt ggtgccgcgc tcggtgtcat cgcggtgatc gttaggcagg
    11041 aagtcgtggc gctggtgatg ggcggcgtgt tcgtgctcga gaccgcgtcg gtcattctgc
    11101 aggtcgcgtc gttcaagctc accggcaaac gtctcttccg catgtcgccg atccaccacc
    11161 actacgagct gaagggctgg gcggagccca aggtcatcgt gcgtttctgg atcctctcgc
    11221 tgctgctcgt gctcgcgggc ctcgccacgt tgaaactgcg atgagcacgg caaccacagg
    11281 cgcacgctac gccgtcatcg cagggctcgg tcgcacggga ctgtcgtgcg cgcgctatct
    11341 gcacgccgag ggctggcgca tcgcggtcac cgacacccgc ccgcgcccgc ccgagctcga
    11401 cgcattgcgc gcgctcgtcg gcgacatcgt cgtgcgcgcc ggcggactcg atacgggcct
    11461 gctcgagggt gcgactctgg tcgtggcctc gccggggctc gcgctcaccg acccgttctt
    11521 cgtcaccgcg cgtgcgcgag gcctcgaggt cgtcggtgac atcgagctgt tcgcgcgtgc
    11581 cgcgcgcgcc ccggcggtcg gcatcaccgg cacgaacggc aagagcacgg tcacgacgct
    11641 ggtcgcccgc atggcccgcc gagcgggact cacggtgcgc gtcggcggca atctcggtga
    11701 gccggcgctc gacctgctgg gcgatccggc cacggccctc tatgtcctcg agctgtcgag
    11761 cttccagctc gagacgacgc gcacgctcga actggccgcc gccaccgtgc tcaatgtcac
    11821 cgcggatcac atggatcgct acgcgaccct cgcggactac gcggcggcga aggcgcgcat
    11881 ctacgcgcgc tgccatgccg cggtgatcaa cctcgacgat ccgctggtcg ccgcgatgcg
    11941 cgcgccggtc gcgcgggacc tgccgcgcac gacgctcacg ttctcgctgc gggcggcgat
    12001 cggtgccgac tacgcgctcg ccgatcgcgg cgcggaggag ggctggtggt tgacgcgtcg
    12061 cggcgagccg ctgatggcgc tcgcggaact gcgcatcccg ggattgcaca acgccgccaa
    12121 tgcgctcgct gcgctcgcgc tcggcgaggc gctcgcgctg ccgcttgcgg cgatgctggc
    12181 ggagctacgc gagttccgcg gcctcgagca tcgcacgcag tgggtcgccg aggtcggcgg
    12241 cgtgcgctat gtgaacgatt ccaagggcac caacgtcggc gcgactctcg ccgcggtgtc
    12301 gggcctgccg ggcaccctcg tgatcatcgc gggcggtgac ggcaagggcc aggacttcgg
    12361 gccgctcgcc gcggcgtttc gcgacaaggt gcgccacgtc gtgctgatcg ggcgcgatgc
    12421 gccggcgatc gccgcggtgc tcgaaggggt ctgcccgctg tcgcgcgcgg agacgctcga
    12481 ggcggcggtg cgcaccgcgg cgagcgtcgc gcgcgcgggc gacacggtgc tgctctcgcc
    12541 ggcgtgcgcg agcctcgaca tgttccgtga ctacacgcag cgcggccgcg tgttcgagca
    12601 ggccgtgaag gagctcgcgg catgagcggg tgccgtccat gaacgccgcc acactcggct
    12661 gggcccgctc gggcagccgc ggcggcgcct tccgcaccga ctgggtgacg gttgcgctgg
    12721 tgctcgcgat cacgctgctc ggtctcgtca tggtgacctc ggcgtccgtt tcgatcgcga
    12781 acaacgagac gggccagccg ttctactacc tcgagcgcca gctcgcgctg ctgctggccg
    12841 ggggcgtggc ggccgtggtg ctgacgcgcg tgcgcaccga gttgctcgag aagctcgcgc
    12901 tgccgctgct gatcgtcgcg atcgcactgc tggtcgcggt gctgatcccc ggtctcggtc
    12961 acgtagtcaa cggcagccgg cgctggatcc gtctgctcgg cttcaacttc caggcctcgg
    13021 agctcgcgcg cctgctcgtg ctgatctatg tcgcgagcta tgccgcgcgg cgggaatcgg
    13081 agctgcgcac gagcttctcg ggactggcgc ggccgctcgg cctgctggcg ctggtcggcg
    13141 tgctgctgct cgccgagccg gacttcggcg cggccaccgt gctgttcgcc accggcttcg
    13201 gcgtgctgtt catggcgggt gcgcggctgc gccacgtgct cgcgatggtg ctggccgcgg
    13261 tagccgcgtt cggcgcgctc gttgcgctgt cgggctatcg catgcgccgc ctcgcgacct
    13321 tcctcgaccc gtgggccgat ccgtacgaca gcggcttcca gctcacgcag tcgctgatcg
    13381 cgatcggccg cggcgaatgg ttcggtgtcg gcctcggcga gagcgtgcag aagctcttct
    13441 acctgccgga ggcgcacacc gacttcctgt tcgccgtgct ggccgaggag ctgggcctcg
    13501 tcggtgtcgt gctgaccgtt ggtctcttca tcgcgctcgc ctggcgcgcc ctgcacatcg
    13561 cgcagctcgc gtccgacgcg gggctcaggt tccaggcctg cctcgcggcc ggcttcggcc
    13621 tgtggatcgg catgcaggcc ttcatcaaca tcggcgtgaa catgggcgcg ctgcccacga
    13681 aggggctgac gctgcccttg atgagctacg ggcgctcgag cctcatcgtg acgatcgcgt
    13741 gggtcggcat cctgctgcgt gtccatcacg aggccgcctc gcgtacgcgc gggccggcga
    13801 cggtgagcgc atgagcggtg gcaccgtgat catcatggcg ggcggcaccg gcgggcacgt
    13861 attcccggcg ctggcgctgg cgcgcatcct gcaggcgcgc ggccacgaca tcgtgtggct
    13921 cgggacgcgc gagggcatcg aggcgcggct ggtgccggct gagggtctgc cgatcgagtg
    13981 gatttccatc ggcggactgc gcggcaaggg catgggcaca ttgctcgcag ctccgttccg
    14041 tcttgcgcgc gcgctatacc agagcctgcg cgcgatgcgc cgccatcgcc cgatcgtcgt
    14101 cgtcggcctc ggcggctacg tcaccggtcc cggcggcctt gcggcctggc tcgcgcgccg
    14161 gccgctcgtc gtgcacgaac agaacgccat cgccggctac accaaccgcg tgctggcgcg
    14221 catcgcgcgc gatgtgctgg cggcctttcc cgatgccttc gacggcgccg tgccggtgag
    14281 cgtgatcggc aacccggtgc ggcgcgagtt cttcgagctc ggcgagcccg cggcgcgcgc
    14341 ggccggtcgc ggtgccgtgc tgcgcctgct ggtggtcggc ggcagccagg gtgcgaacaa
    14401 actgaacgcg gtcgtgccat tcgcgatcgc gcgcctc
//
LOCUS       DAOQVY010000055        14322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_4791_length_14322_cov_5.947782, whole genome shotgun sequence.
ACCESSION   DAOQVY010000055 DAOQVY010000000
VERSION     DAOQVY010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14322
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4791_length_14322_cov_5.947782"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PKN91_06770"
     CDS             complement(<1..734)
                     /locus_tag="PKN91_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27809.1"
                     /translation="MTLRPYYRTFDLPWDIDPEESERLRKFLIVALVTVTLGAIVIPF
                     LPVPDRPVQEEVVPQRLAKVMLDYQPPPPPPPPPAPKEEPKPETKVAAITPKPVPQPV
                     DRTEEARKKAQRAVNQFHDELADLRQNLPVVPVEQTRNLTGEVGADSRAERSLITSKV
                     GAASGGIASTSSSRGFGAGAGSLTGHDATRVGLPPGVPVGGRADAASRSGSSGKASRS
                     REEIELVFDRNKSAIYALYSRALREQP"
     gene            complement(731..1252)
                     /locus_tag="PKN91_06775"
     CDS             complement(731..1252)
                     /locus_tag="PKN91_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNS27810.1"
                     /translation="MSMSGRARRMAKSHERHKGALELNLIPMIDILTVLVMFLLVYST
                     EVEIIQNTKGIEIPQSIAEAQPKDSVVLMVTTTDLFVQGEHVTSLAEIQASDSPLIEP
                     LRAALKRPMLVGRAMTEKELAAREITILGDKAIPYEVLKKVMATCTDADYGRISLATL
                     QKEKPAPAGFKPA"
     gene            complement(1249..1776)
                     /locus_tag="PKN91_06780"
     CDS             complement(1249..1776)
                     /locus_tag="PKN91_06780"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNS27811.1"
                     /translation="MRRSSYEARKLQRRGRRPSELLLVPMIDIFIVLVTFLLMTAVFS
                     RTVVLELNLPARLADFREPPQGLQLEVIVRKDELIVADRNSGPLAPLPSSAAGYDFDG
                     LTGYLQRVKARFPEMTSATILLESDTPYDTLVQVMDKVRVVEVSQDGMTVQAELFPDI
                     SIGDAPQAAAGGGRT"
     gene            complement(1793..2395)
                     /locus_tag="PKN91_06785"
     CDS             complement(1793..2395)
                     /locus_tag="PKN91_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNS27812.1"
                     /translation="MIPIAVVLVLGLAIALERFIYLWNVGRQNRSLWSEITPLLQRGD
                     FRAAVQLTSKSSSAIGQILNYGLARVATARRRDDIEKAMEESLMEVVPKIERRTHYLA
                     TFANLATLLGLLGTVFGLIHAFAAVATVNPAEKANLLSASISVAMNCTAFGLLTAVPL
                     LFIHAWLQSRTTALIDSLEMASVKFLNSITERRPDAPAAA"
     gene            complement(2519..2992)
                     /locus_tag="PKN91_06790"
     CDS             complement(2519..2992)
                     /locus_tag="PKN91_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27813.1"
                     /translation="MKTLPLLTVSVLLMPCAWAQEPLPTPESLEPGVDTSARPAVPAG
                     GQTPAATAPTPAAPPAAAPADVSRAAAGQRGTRGVMDRIELDTTQITGNRELPKVLYI
                     VPWKQSDLGDLMGRPANSLLDEVLAPVDRDVFRRENRYYDALQPDAAQRADKAEK"
     gene            complement(2989..6399)
                     /locus_tag="PKN91_06795"
     CDS             complement(2989..6399)
                     /locus_tag="PKN91_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS27814.1"
                     /translation="MSVARQFAALCVLALAVASPPAALAAQKPKAAPTIKDLRARPLD
                     LQKSAAVPASASRAMDNYRRFLELQNTDPELRAEALRRLGDLNLEAGELERMQSEVNA
                     IDLASAEAIVLYTTLLKAYPNYSRNDQVLYQLARAYETTGQPEKALATLDDIVSRYPS
                     APQMDEIQFRRGEMLFSARDYGGAEAAYGQAMKFGESSPYFEQSLYKHGWSLFKQSRN
                     EESLESFGGVLDAMLVGQGGRERRFDGLSRADRELVDDTLRVMAITFSYLDGAESLDA
                     HIARRGTPPYSHLLYSRLGDLYVDKERYQDAASTYRAFVNRDPNNEYAPGLSMQAIEA
                     YKKGGFTDLVLDGKREFVERYRFAGPFWLGRDRAAYPTVTAELKSNLKDVAQYHHATA
                     QASKKVEDYQQAARWYRDYLESFPDDPDSAATNYLLAETLFESHQYLDAAGEYQRTAY
                     DYPRSAQSAAAGYAALVALQRHEEALPAADRLAVHALAVDSGVRFATTFPEHPDSAGV
                     LTRAAQDVFAARDLPRAITLAETLLARAPPVDTAKQRIAWTIIGQSNFDLLYFDKAEA
                     AFVNARTLAPPGDPERADLTERLAASVYRQAEARRTSGDASGAVDAFLRVAVVAPDSQ
                     IRSTATYDAAALLVTLKEWGRAVPVLESFRRDFPEDKLQPEVTRSLAVAYAETGRAGE
                     AAAEFERIAASPGEDPAVRREAVVQAADLHEKAGNSARSEAMLEKFVADYPTPVLDAI
                     EVRQRLADAAARRGDVAKQQSWQRAIILADASAGAERSDRTRFLAAHAQLALAAPARD
                     AFRGVRLVAPLKNSLAQKRSSLEIALAAYKSAADYRIADVTTSATFEMAELYRTLARD
                     LMESERPKNMEDEALEQYDLLLEEQAFPFEEQAIAVHQANAARAREGVYNDGVKASYA
                     ALAQLNPGRYGKTELAQGVVDALTLLEPPPPVPVVVDGETPAAPAPVPAPPPARALAD
                     FDRAVGLARAGDNAGAALEFGQLSAAVPQYAAVFFNLGVAQLNQNRLDDAEAAFRAAL
                     ERNPASAEAAAELGLTLRMAGRFEQARAAYEQALAAREGFASAHRNYAVLLDLYLQEP
                     APALAHLERYRELTGEDKPVSGWIAELRQRAGSAPPAADADPVAGAEP"
     gene            complement(6396..8321)
                     /locus_tag="PKN91_06800"
     CDS             complement(6396..8321)
                     /locus_tag="PKN91_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27815.1"
                     /translation="MRSLVLALVAATVVATAGNDDAWAADTGDRLPVTRVQDLHYGDV
                     LFHFFQDENFEALTRLLAYGHWDRLPHHLAEAELLAGGMYLSLGMHNEAGARFERLLT
                     AETPQGVRNRAWFYLGKVWYARGYDDKAERALRSVTGTLPPDLEAEKTHLLANLMMHG
                     GRYDEAVALLDAVQGSPDWVAYARFNLGVAMVRKGQLADADRYLTGVGTMAAVSPEML
                     ALKDKANLALGFAYLQADQPANAKLALERVRLAGPQSNKALLGAGWASAALGDFEGAL
                     APWLELRDRNLLDAAVQESFLAVPYAYGKLGANAQAADYYETAMREFDREGESLDGAI
                     ASIRSTRLLDELLAQESEERQGWFWQLRDLPDAPQSRYLYAVLAGHDFQEGLKNYRDL
                     AFLNRRLGAWLESMVAFDDMIDARQKAHAERLPRADGLLAQDAPARIERERGELESKL
                     DAIERNHDAAALGTPTERDQWARIQRIEQALANGDVGVAGPELEALEDKLRLVKGVLS
                     WRLDEAMRARVYQQRREIKDLDAALREAQRRWVRVERARKDVPTNTGEFEGRIAALNA
                     RLAALQSRLESARDRQNEYLATRAIQELESQQARLAAYRVQARFALATIYDRAANEPA
                     PAPQAPVVPPPAIEGPP"
     gene            complement(8322..8927)
                     /locus_tag="PKN91_06805"
     CDS             complement(8322..8927)
                     /locus_tag="PKN91_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27816.1"
                     /translation="MSASVDTSEIHMPTVARPIAALTRLLLPALLAGLAGAAYAQEAV
                     APAADAPAETRALDSEVQDLKKDVVDLNRELFILEEELLFPANTQVAVFVSLDVGEFF
                     ALDSVQLKIDDREVSNYLYTPREAEALLKGGVQRLYVGNLKTGTHELVAFFAGKGPNG
                     RDYRRGASLNFEKSVGAKYLELRISDRQRKAQPEFEIKDWE"
     gene            complement(8924..9424)
                     /locus_tag="PKN91_06810"
     CDS             complement(8924..9424)
                     /locus_tag="PKN91_06810"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNS27817.1"
                     /translation="MLIDRIVPAVLSLCAAFAASAADSPLVGREAPDFALRAMAGDNV
                     RLSEARGQVVVLSFFGSRCNPCRAQLAELDAVYRTYGPAGLTVYGISIDDDAEKARQF
                     SASVGVGFPMLADPQKAVGREYAIDRLPTVVIVDRAGKVRYVHRDPESRSEPVYVREL
                     RRLLDE"
     gene            complement(9604..11196)
                     /locus_tag="PKN91_06815"
     CDS             complement(9604..11196)
                     /locus_tag="PKN91_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27818.1"
                     /translation="MLIRHKPPRELGLDEPLRHPDHRPPVTRRELISRGFQTGAAVVA
                     APSVLSLLAPRDAHGALASDIEALKEACGITDGAGKIPFIAFDLAGGANLSGSEVLIG
                     GPGGPEDFLSTAGYGKLGLPGDMVPNAASATNTFIDTSLGLPWHSDGGMLRGILSKAS
                     VGAQAFTNGAVIAARSENDTANNPHNPMYGIQKCGADGQLLTLIGSRASDSGGNSMSP
                     MPYDLAYRPTKVDRTSDVTGLVDTGKLAELMAQNDAVAVLESMVRLSGDKLGRVNTNV
                     SRDAAIKTLVECGYTKTAYLADRFGQPANLNPELDQRIKGATGIFTDQEFDSDGEFEK
                     TAAVMKMVIEGYAGAGTITMGGYDYHGQGRATGEVRNFRAGQCIGACLEFAHRAGKPL
                     MIYVFSDGSLSSANQVDNTVNGRGKFMWVSDNQSTASSFFLVYNPGGRPVVTPSGSSF
                     RSGPQIGYYTADGSVANNSHPGANAVNLLVNTVLLNYLALHQQIGQFTTLFPNHGLGS
                     GAQLDSLAVFAPIVDAVITNPL"
     gene            complement(11216..13645)
                     /locus_tag="PKN91_06820"
     CDS             complement(11216..13645)
                     /locus_tag="PKN91_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNS27819.1"
                     /translation="MNRNVLLATVLAALAACSGSGAPTEVNPVTTPPPVADYTGPAPA
                     NADVQAFRINLWENIKASNRCGSCHNAGGQSPTFARNDDVNLAYQAAVALVDLTQPDQ
                     SRLVTKVAGGHNCWLASASACGDTMTVWIRNWAGSLAGGGKTIQLQEPPIREVGDSKT
                     FPDSSSLYATTILPLVRGQGQCLRCHTPASATAQAPYFAAQDADAAYAAARPKINLDA
                     PELSRLVVRLREESHNCWTSSCLNDASTMETAVRAFANAIAVTPVDPTLRLSKALTLY
                     DGTVAAGGNRYESAQIALYEFKTGMGTTVYDTSGVEPAANLTMSGSIEWVGGWGLNVR
                     EGGKAQGTTAGSRKLADMIKTTGEFTIEAWAAPGNVVQEDAYIVSYSGGTMANNATLA
                     QFAYQYQAMTRSSETDANGEPALLTRAADEDAQASLQHVVLTYDPVNGQRIYVNGQYT
                     GDTDGSGGALGDWDDSFALVLGNDPSGARQWTGVLRLVSVHNRALTQPQIQQNFEAGV
                     GERYFMLFNVSELVDLPKSYVMFEASQYDSYSYLFTNPTFISLDPAVEPAGIPLKGMR
                     IGLNGQEARVGQAYIPLDLTVGAAGYDPTTGQLLSSTGTVIGLEKGPENDLFFLTFER
                     LGDQTRAYPEEPIPDPPVLADSENPEVGLRTFDEINQTMSNITGVPITRASVASTYGL
                     VRQALPSVESIDTFGSSQQMGVAQLAISYCDAMVDDTALRTGFFGGGGAIDVNSAAGR
                     NAMISSIISRAVGTNVASQPDDTEVRTELNALVTKLATTCAGSCNATRTATIAKASCA
                     AAIGSAAMLLQ"
     gene            complement(13656..>14322)
                     /locus_tag="PKN91_06825"
     CDS             complement(13656..>14322)
                     /locus_tag="PKN91_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27820.1"
                     /translation="AAATAGLITTRASSEAFFIDGTNRAMFRFTLMNHLCRDLEQVQD
                     PTLPPDRIRQDVSRSPGGDSRVYLNNCISCHTGMDPMTQAFAYYDFDETQQRLVYTPG
                     TPNAKYFANADNFKPGYVTPDDSWDNYWRSGHNRLLGWGWGTTTTTGAGAGAKSLGDE
                     LANSEAFASCQVQKVFKTVCFRAPQNAADRTRAAAIAQSFRNSGYRLKTVFAETAAYC
                     MGN"
BASE COUNT         2124 a         4820 c         4980 g         2398 t
ORIGIN      
        1 ggctgctcgc gcagggcccg gctgtacagc gcgtagatag ccgacttgtt gcggtcgaac
       61 acgagctcga tctcctcgcg gctgcgcgag gccttgccgc tcgagcccga gcggctggcg
      121 gcatcggcgc ggccgccgac cggcacgccg ggcggcagcc ccacgcgcgt cgcgtcgtga
      181 cccgtcaggc tgcccgcgcc cgcgccgaag ccgcggctcg aactggtcga ggcgatgccg
      241 ccgctcgccg cgcccacctt cgaggtgatc agcgagcgct cggcacggct gtcggcgccg
      301 acttcaccgg tgaggttgcg ggtctgctcg accggcacga caggcaggtt ctgccggagg
      361 tccgcgagct cgtcatggaa ctggttgacc gcccgctgcg ccttcttgcg cgcttcctcg
      421 gtccgatcga ccggctgcgg cacgggcttc ggggtgatgg ccgccacctt ggtctcgggc
      481 ttcggctcct ccttgggcgc aggcggcggc ggcggtggcg gcggcggctg gtagtcgagc
      541 atgaccttcg cgagtcgctg cggcaccacc tcctcctgca ccgggcgatc cggcacgggc
      601 aggaacggga tgacgatggc gcccagcgtg accgtgacga gcgccacgat caggaacttg
      661 cgcaggcgct cgctctcctc gggatcgatg tcccagggca gatcgaaagt ccggtagtac
      721 ggcctcagcg tcacgcgggc ttgaagccgg ccggggccgg cttctccttt tgcagcgtgg
      781 cgagcgaaat gcgcccgtaa tcggcatccg tgcaggttgc catgaccttc ttcagcacct
      841 cgtaggggat ggccttgtcg ccgaggatcg tgatctcgcg cgcggcgagt tccttctcgg
      901 tcattgcccg cccgaccagc atcggccgct tgagcgcggc ccgcagcggc tcgatcagcg
      961 gcgagtcgga cgcctggatc tcggcgagcg acgtgacgtg ctcgccctgc acgaacaggt
     1021 cggtggtggt caccatcaat acgacggagt ccttcggctg cgcctcggcg atcgactgcg
     1081 ggatctctat gcccttggtg ttctggatga tctcgacctc ggtcgagtac accagcagga
     1141 acatcaccag cacggtcagg atgtcgatca tcgggatcag gttgagctcg agggcgccct
     1201 tgtggcgctc gtggctcttc gccatgcggc gtgcgcggcc cgacatgctc atgttcggcc
     1261 tccgccggcg gcggcctgtg gcgcgtcacc gatcgaaatg tccgggaaca gttccgcctg
     1321 cacggtcatc ccgtcctgcg agacttcgac tacgcgcacc ttgtccatca cctgcacgag
     1381 cgtgtcgtac ggggtgtcgg actcgaggag gatggtggcg ctcgtcatct cgggaaacct
     1441 cgccttcacg cgctgcaggt agccggtgag gccgtcgaag tcatagcccg ccgcgctgct
     1501 tggcagtggc gcgagcggcc cgctgttgcg gtcggcgacg atcagctcgt ccttgcgcac
     1561 gatcacctcg agctgcaggc cctgcggcgg ctcgcggaaa tcggcgagcc gggccggaag
     1621 gttcagctcg aggaccacgg tccgcgagaa cacggcggtc atcagcagga aggtcacgag
     1681 cacgatgaag atgtcgatca tcggcacgag cagcagctcg gacggccggc gtccgcgccg
     1741 ctgcagcttc cgggcctcgt aggaggacct gcgcatgggc ggctcctcgc gcctaggccg
     1801 cggccggggc gtccggacga cgctcggtga tcgagttcag gaacttgacc gaggccatct
     1861 ccaggctgtc gatcagcgcg gtcgtgcgcg actgcagcca ggcatggatg aacagcagcg
     1921 gcaccgcggt cagcaggccg aaggccgtgc agttcatcgc caccgaaatg ctggcggaca
     1981 gcaggttcgc cttctccgcc ggattcaccg tggcgacggc cgcgaacgcg tggatcaggc
     2041 cgaacacggt tccgagcagg ccgagcagcg tcgcgagatt ggcgaaggtc gcgaggtagt
     2101 gagtgcgccg ctcgatcttc ggcaccactt ccatcaggct ctcttccatc gccttctcga
     2161 tgtcgtcacg gcggcgcgcc gtcgcaacgc gcgcgagacc gtagttcagg atctggccga
     2221 tcgcggaact ggatttcgac gtgagctgca cggccgcgcg gaagtcgccc cgctgcagca
     2281 acggggtgat ttccgaccac agcgagcgat tctgccgccc gacattccac aggtagatga
     2341 agcgctcgag cgcaatcgcg agcccgagca ccaggaccac ggcgatgggg atcatccaat
     2401 gtcccccgcc ctggaagaag cgaactatcg aactatagag atccatcgaa gactctcctc
     2461 gaaaaacggc aagccaagca ttcccaaact gaatgacata agttcaaaaa gctgcgacct
     2521 atttctcagc tttgtcggcg cgctgcgcgg catccggctg cagcgcgtcg taataacggt
     2581 tttcgcggcg aaacacgtca cggtccacgg gcgccagcac ctcgtcgagc aggctgttgg
     2641 ccggccgacc catcaggtcg cccaagtccg actgcttcca cggcacgatg tacagcacct
     2701 tcggcaattc gcgattgccg gtgatctgcg tcgtatcgag ttcgatgcgg tccatcacgc
     2761 cgcgcgtgcc tcgctggccg gccgcggcgc gcgacacatc ggcgggcgca gcggcgggag
     2821 gcgctgccgg ggtgggcgcc gtggcggcag gggtctgccc gccggcgggc accgcaggcc
     2881 ttgctgaggt atcgaccccc ggttcgaggg actccggggt cggcagcggc tcctgcgccc
     2941 aggcgcaagg catgagcaac acgctcacgg tgaggagggg cagtgtcttc atggctcggc
     3001 tcctgcaacg ggatcggcat cggcggcagg tggtgcgctg ccggcacgct ggcgcagttc
     3061 cgcgatccag ccgctgaccg gcttgtcctc gccggtcagc tccctgtagc gctcgaggtg
     3121 tgccagcgcc ggcgccggct cctgcaggta caggtcgagc agcaccgcgt agttgcggtg
     3181 cgccgacgcg aagccctcgc gcgcggccag cgcctgctcg tacgcggcgc gcgcctgctc
     3241 gaaccggccc gccatgcgca gcgtgaggcc gagctctgcc gccgcctccg cgctcgccgg
     3301 attgcgctcg agcgccgcac ggaacgccgc ctcggcgtcg tccagccgat tctggttgag
     3361 ctgtgcgaca cccaggttga agaacacggc cgcgtactgc ggtaccgccg ccgacagctg
     3421 gccgaactcg agcgctgcac cggcgttgtc gccggcgcgc gccagaccca ccgcccgatc
     3481 gaaatcggcc agggcccgcg ccggcggcgg cgccggcacc ggggcgggcg cggccggtgt
     3541 ttcaccgtcc accaccaccg gcacgggcgg cggcggctcg agcagggtca gcgcatcgac
     3601 gacgccctgc gccagctcgg tcttgccata gcggccggga ttcagctgcg cgagtgcggc
     3661 atagctcgcc ttcacgccat cgttgtagac gccctcgcgc gcccgcgcgg cattcgcctg
     3721 atgcacggcg atcgcctgct cctcgaacgg aaacgcctgc tcctcgagca gcagatcgta
     3781 ctgctcgagc gcctcgtcct ccatgttctt cggacgctcg gactccatca ggtcgcgcgc
     3841 gagcgtgcgg tagagctccg ccatctcgaa ggtcgcgctc gtcgtgacat cggcgatgcg
     3901 gtaatcggcc gccgacttgt aggccgcgag ggcgatctcg agcgagctgc gcttctgcgc
     3961 gaggctgttc ttcagcggcg cgacgaggcg cacgccccgg aacgcatcgc gcgccggcgc
     4021 agcgagcgcg agctgggcat gggcggcgag aaagcgggtg cgatcgctgc gttcggcgcc
     4081 ggcgctcgca tccgcaagga tgatggcgcg ctgccaggac tgctgcttcg ccacgtcgcc
     4141 gcgcctggcc gcggcgtcgg ccagccgctg gcgcacttcg atcgcgtcaa gcaccggagt
     4201 cgggtaatcg gcgacgaact tctcgagcat ggcctccgaa cgcgcggaat tgcccgcttt
     4261 ctcgtgcagg tccgcggcct gcaccacggc ctcgcggcgc accgccggat cctcgcccgg
     4321 cgaggccgcg atacgctcga attcggcggc ggcttcgccc gcgcgcccgg tctccgcata
     4381 ggcgaccgcg aggctgcgcg tgacctcggg ctgcagcttg tcctcgggga agtcgcgccg
     4441 gaaggattcg agcaccggga ccgccctgcc ccattccttc agcgtgacca gtagcgcggc
     4501 ggcatcgtag gtcgccgtcg agcggatctg cgagtccggc gccacgaccg cgacgcgcag
     4561 gaacgcgtcg accgcgcccg atgcatcacc ggaggtgcgc ctggcttcgg cctgcctgta
     4621 gacggaagcc gccagccgct cggtgagatc ggcgcgctcc gggtcgccgg gcggggccag
     4681 cgtgcgcgca ttgacgaagg ccgcctcggc cttgtcgaag tacagcaggt cgaagttcga
     4741 ctggccgatg atcgtccagg cgatgcgctg cttcgcggtg tcgaccggtg gcgcgcgcgc
     4801 aagcagcgtt tcggcgagcg tgatggcccg tggcaggtcg cgcgccgcga acacatcctg
     4861 cgcggcgcgc gtcagcacgc ccgcgctgtc gggatgctcg gggaacgtgg tcgcgaacct
     4921 gacccctgaa tccaccgcca gcgcatgcac cgcgagccgg tcggcggcag gcagcgcctc
     4981 ctcgtgccgc tggagggcga cgagtgcggc atagccggcg gcagcggact gcgcactgcg
     5041 cggataatcg taggccgtgc gctggtactc gcccgccgca tcgaggtact ggtggctctc
     5101 gaacagcgtc tcggcgagca gatagttggt ggcggccgag tccggatcgt ccgggaatga
     5161 ctccaggtag tcgcggtacc agcgcgccgc ctgctgatag tcctcgacct tcttcgaggc
     5221 ctgcgcggtc gcgtggtggt actgcgcgac gtccttcagg ttggacttca gctccgccgt
     5281 gaccgtgggg taggcggcgc gatcgcggcc cagccagaac gggccggcga agcgatagcg
     5341 ctcgacgaac tcgcgcttgc cgtcgagcac gaggtcggtg aagccgccct tcttgtaggc
     5401 ctcgatcgcc tgcatcgaca ggcccggcgc gtactcgttg ttcgggtcgc gattgacgaa
     5461 ggcgcgatag gtcgacgcgg cgtcctggta gcgctccttg tccacgtaca agtcaccaag
     5521 gcgcgagtac agcaggtgtg aataaggcgg cgtgccgcgc cgcgcgatgt gcgcatcgag
     5581 cgattcggcg ccgtcgaggt aggagaacgt gatcgccatg acgcgcagtg tgtcgtcgac
     5641 gagttccctg tcggcgcgcg acaggccgtc gaagcgccgc tccctgccgc cctgcccgac
     5701 cagcatggcg tcgagcacac cgccgaacga ctcgaggctc tcctcgttgc gcgactgctt
     5761 gaacagcgac cagccgtgct tgtagaggct ctgctcgaag tatggcgacg actcgccgaa
     5821 cttcatggcc tggccatagg ccgcttccgc gccgccatag tccctggccg aaaacagcat
     5881 ctcgccgcgg cggaactgga tctcgtccat ctgcggcgcg gacggatagc gactgacgat
     5941 atcgtcgagc gtcgcgagcg ccttctctgg ttgcccggtc gtctcgtagg cgcgcgcgag
     6001 ctggtagagc acttgatcgt tgcgcgaata gttcgggtag gccttgagca gggtcgtata
     6061 caacacgatc gcctcggcgc tcgccaggtc gatcgcgttc acctcgctct gcatgcgctc
     6121 gagttcgccg gcctcgagat tcaggtcgcc gagtcgccgc agcgcttccg cgcgcagttc
     6181 gggatcggtg ttctgcagct cgaggaagcg gcggtagttg tccatcgcgc gcgatgcgct
     6241 agcgggcacc gccgcggatt tctgcaggtc gagcggacgc gcacgcaggt ccttgatggt
     6301 gggcgcggcc ttcggcttct gcgcggccag ggcagccggg ggcgacgcga ccgcgagcgc
     6361 gagcacgcac agcgccgcga actggcgggc gacgctcacg gcgggccctc gatggcgggc
     6421 ggcggcacga caggcgcctg cggcgcgggc gccggctcgt tggccgcgcg atcgtagatc
     6481 gtcgcaagcg cgaagcgcgc ctgcacccga tacgcggcga gccgcgcctg ctgcgattcg
     6541 agttcctgga tcgcgcgcgt cgcgaggtac tcgttctgcc tgtcgcgggc gctttcgagc
     6601 cgcgactgca gcgcggcaag ccgcgcattc aatgccgcga tgcgcccctc gaactcgccg
     6661 gtgttggtcg gcacgtcctt gcgcgcgcgc tcgacacgca cccagcgtcg ctgtgcctcg
     6721 cgcagcgcag cgtcgagatc cttgatctcg cgccgctgct ggtagacgcg cgcgcgcatt
     6781 gcctcatcga gccgccatga cagcacgccc ttgacgagcc gcagcttgtc ctcgagtgcc
     6841 tcgagttccg ggccggcgac ccccacgtcg ccattggcga gcgcctgctc gatacgctgg
     6901 atgcgcgccc actggtcacg ctcggtgggc gtaccgagtg ccgcggcgtc gtgattgcgc
     6961 tcgatcgcat cgagcttcga ctcgagctcg ccgcgctcgc gctcgatgcg ggcgggtgcg
     7021 tcctgcgcga gcagcccatc ggcgcggggc agtcgctcgg cgtgcgcctt ctggcgggca
     7081 tcgatcatgt cgtcgaaggc gaccatgctt tccaaccatg cgcccaggcg ccggttgagg
     7141 aacgcgaggt cgcggtagtt cttcaggcct tcctggaaat cgtgtccggc cagcacggcg
     7201 tagaggtagc gcgactgcgg ggcgtccggc aggtcgcgca gctgccagaa ccagccctga
     7261 cgctcctcgc tctcctgggc gagcagctca tcgagcaacc gggtcgaacg gatgctcgcg
     7321 atcgcgccgt cgagcgactc gccctcgcgg tcgaactcgc gcatcgcggt ttcgtagtag
     7381 tcggcggcct gagcattcgc gccgagcttg ccgtaggcgt acggcaccgc aaggaaggac
     7441 tcctgcaccg cggcgtcgag caggttgcga tcgcgcagtt cgagccatgg cgcgagcgcg
     7501 ccctcgaaat caccgagcgc cgcgctcgcc caaccggcgc cgagcagcgc cttgttcgat
     7561 tgcgggccgg caaggcgcac gcgctcgagc gcgagcttcg cgttcgccgg ctgatcggcc
     7621 tgcagataag cgaagcccag cgcgagattc gccttgtcct tgagcgcgag catctcgggc
     7681 gaaacggccg ccatggtgcc gacaccggtg aggtagcggt cggcgtcggc cagctggccc
     7741 ttgcggacca tggcgacccc cagattgaag cgcgcgtagg ccacccagtc ggggctgccc
     7801 tggacggcgt cgagcagcgc gactgcctcg tcgtagcgcc cgccgtgcat catcagattg
     7861 gcgagcaggt gcgtcttctc ggcctcgagg tccgggggca gcgtgcccgt gacgctgcgc
     7921 agcgcccgtt cggccttgtc gtcgtaaccg cgtgcgtacc agaccttgcc gaggtagaac
     7981 cacgcgcggt tgcgcacgcc ctgtggcgtc tcggcggtca gcagccgctc gaaccgggcg
     8041 ccggcctcgt tgtgcatgcc gagggagagg tacatgccgc cggccagcag ctcggcttcg
     8101 gcgagatgat gcggcagccg gtcccagtgc ccgtaggcca gcagacgcgt cagcgcctcg
     8161 aagttttcgt cctggaagaa atggaacagc acgtcgccgt agtgcaggtc ctgcacgcgg
     8221 gtgaccggca gcctgtcccc ggtgtccgcc gcccacgcat catcgttgcc ggcggtcgcg
     8281 acaaccgtgg ccgcaacaag tgcgaggacg aggctccgca tctactccca gtccttgatc
     8341 tcgaattccg gttgcgcctt gcgctggcgg tcgctgatgc gcagctcgag atacttggca
     8401 ccgacgctct tctcgaaatt cagcgaggcg ccgcgccggt agtcgcgccc gttcggtccc
     8461 ttgcctgcga agaaggccac cagctcatgg gtcccggtct tcaggttgcc gacgtacagg
     8521 cgctgcacgc cgcccttcag caacgcctcg gcttcgcgcg gcgtgtagag ataattcgac
     8581 acttccctgt cgtcgatctt cagctgcacc gagtcgagcg cgaagaactc gccgacgtcg
     8641 agcgacacga acaccgcgac ctgcgtgttc gccgggaaca gcagttcttc ctcgagaatg
     8701 aacagttcgc ggttgagatc gaccacgtcc ttcttgaggt cctggacctc gctgtcgagc
     8761 gcgcgggtct ccgccggcgc gtcggccgcc ggagcgacgg cctcctgggc gtaggcggcg
     8821 ccggccagcc cggcgagcag tgccgggagc agcagtcgtg tcagcgcggc gatcggtctg
     8881 gcaacggtag gcatgtggat ctcgctggtg tctacactgg cgctcattcg tcgagcaggc
     8941 ggcgcagctc gcggacatac accggttcgc tgcgggattc cggatcacga tgcacgtagc
     9001 gcacctttcc cgcgcggtcg acgatcacga ccgtgggtag ccggtcgatc gcgtactcgc
     9061 gcccgaccgc cttctgcggg tcggcgagca tcgggaagcc gacgccgacg ctcgccgaga
     9121 actgcctcgc cttttcggcg tcgtcatcga tgctgatgcc gtacacggtc aggccggccg
     9181 ggccgtaggt gcggtacacc gcatcgagct ccgccagctg ggcacggcac gggttgcagc
     9241 ggctgccaaa gaacgacagc acgacgacct ggccccgtgc ttccgacagg cgcacgttgt
     9301 cgccggccat ggcgcgcagc gcgaaatcgg gcgcctcgcg gccgaccagc ggcgagtccg
     9361 ccgcgcttgc ggcgaacgcc gcgcacagcg acagcaccgc gggaacgata cgatcgatca
     9421 acaatgcagc cctcatgaca gcgcgctcac agcaagcgga acaggcgcgc gcggcgcata
     9481 tgaccgtcgc atgcgcactt tacaatgtca acgtagcgcg ccggcaattc gcgcggcaga
     9541 cgcatgacct gccgcacaaa tcagccggcc cgcgccggcc gtcggcaaag cgcgacaggc
     9601 gcgtcagagc ggattggtga tcaccgcatc gacgatcggt gcgaacaccg cgaggctgtc
     9661 gagctgggcg cccgaaccga gcccgtggtt cgggaacaag gtcgtgaact ggccgatctg
     9721 ctggtgcagg gccaggtaat tgagcagcac ggtgttgacc agcaggttga ctgcgttggc
     9781 gcccggatgc gagttattgg ccaccgagcc gtccgccgtg taatagccga tctgcgggcc
     9841 gctgcggaac gaactgccgc tcggcgtgac gaccggccgc ccgcccgggt tgtacaccag
     9901 gaagaacgac gacgcggtcg actggttgtc gctcacccac atgaacttgc cgcggccatt
     9961 gaccgtgttg tcgacctggt tggcggacga cagcgaaccg tcgctgaata cgtagatcat
    10021 gagcggcttg ccggcgcgat gcgcgaactc gaggcaggcg ccgatgcact ggccggcgcg
    10081 gaagttgcgc acctcaccgg tcgcgcggcc ctggccgtgg tagtcatagc cgcccatcgt
    10141 gatcgtgccg gcgccggcat aaccctcgat caccatcttc attaccgcgg cggtcttctc
    10201 gaactcgccg tccgagtcga actcctggtc ggtgaagatg ccggtcgcac ccttgatgcg
    10261 ctggtcgagt tcgggattca ggtttgcggg ctggccgaag cggtcggcga ggtaggcggt
    10321 cttcgtgtaa ccgcattcca cgagcgtctt gatggcggca tcccggctca cgttcgtgtt
    10381 gacgcgaccg agcttgtcgc cgctcaggcg caccatcgac tcgagcaccg cgacggcatc
    10441 gttctgcgcc atcagctcgg cgagcttgcc ggtatcgacg aggccggtga cgtcgctcgt
    10501 gcgatcgacc ttggtcggcc ggtatgcgag gtcatagggc atcggcgaca tcgaattgcc
    10561 gcccgagtcg gacgcacgcg aaccgatcaa cgtcagcagc tggccgtcgg cgccgcactt
    10621 ctggatgccg tacatcgggt tgtgcggatt gttcgcagtg tcgttctcgg agcgagcggc
    10681 gatcaccgcg ccgttggtga acgcctgcgc gcccacgctg gccttcgaca ggatgccgcg
    10741 cagcatgccg ccgtcggaat gccacggcag gccgagcgag gtatcgatga acgtattggt
    10801 cgcgctcgcc gcgttcggca ccatgtcgcc gggcaggccg agtttgccgt agcccgcggt
    10861 cgacaggaaa tcctccgggc cgcccgggcc gccgatcagc acttccgaac cactcaggtt
    10921 ggcgccgccc gccaggtcga aggcgatgaa cgggatcttg ccggcaccat cggtgatgcc
    10981 gcaggcctcc ttgagcgcct cgatgtcgga tgcgagcgcg ccgtgcgcgt cgcgcggcgc
    11041 gaggagactc agcaccgacg gcgcggccac gaccgcggcg ccggtctgga agccgcgcga
    11101 aatcagctcg cgccgggtca ccggcgggcg gtgatcgggg tgccttaacg gctcatcgag
    11161 gccgagttcg cgggggggct tgtggcggat cagcatggtg gattctccct cgaacctatt
    11221 gcagcagcat cgcggcgctg ccgatggcgg cggcgcagga cgccttggcg atcgtcgcgg
    11281 tacgggtcgc gttgcagctg ccggcgcagg tcgtcgcgag cttggtgacc agggcgttca
    11341 gctcggtgcg cacttcggtg tcgtccggct gggacgcgac gttggtgccg accgcgcgcg
    11401 agatgatgct gctgatcatc gcattgcggc ccgccgcgct gttcacgtcg atcgcgccgc
    11461 cgccaccgaa gaagccggtg cgcagcgccg tgtcgtcgac catcgcgtcg cagtaggaga
    11521 tcgcgagctg cgcgacgccc atctgctgcg acgaaccgaa ggtatcgatc gactcgaccg
    11581 acggcagtgc ctgcctgacg aggccgtagg tgctggcgac cgacgcccgc gtgatcggca
    11641 cgccggtgat gttcgacatc gtctggttga tttcgtcgaa ggtgcgcagg ccgacttccg
    11701 gattctcgct gtcggcgagg accggcgggt cgggaatcgg ctcttccgga tacgcgcgcg
    11761 tctggtcgcc gaggcgctcg aacgtcagaa agaacaggtc gttctcgggc cccttctcga
    11821 gcccgatcac cgtgcccgtc gaggacagca gctggccggt cgtcgggtcg tagcccgcgg
    11881 cgccgaccgt caggtcgagc gggatgtagg cctggccgac gcgcgcctcc tggccgttca
    11941 ggccgatgcg catgcccttc agcggtatgc ccgccggctc gacggccggg tcgaggctga
    12001 tgaaggtcgg gttcgtgaac aggtagctgt agctgtcgta ctggctcgcc tcgaacatga
    12061 cgtagctctt cggcaggtcc accagttcgc tgacattgaa cagcatgaag tagcgctcgc
    12121 cgactccggc ctcgaagttc tgctggatct gcggctgggt cagcgcgcgg ttgtgtaccg
    12181 acacgaggcg cagcacgccc gtccactgcc gggcacccga cggatcgttg ccgagcacga
    12241 gcgcaaagct gtcgtcccag tcgccgagcg cgccgccgct gccgtccgtg tcgccggtgt
    12301 actggccgtt gacgtagatg cgctggccgt tcacggggtc gtaggtcagc acgacgtgct
    12361 gcagggacgc ctgggcatct tcgtccgccg cacgggtgag cagggccggc tcgccattcg
    12421 cgtcggtctc gctgctgcgg gtcatcgcct ggtactggta ggcgaactgc gcgagtgtcg
    12481 cgttgttggc catcgtgccg cccgagtaac tgacgatgta ggcgtcttcc tgcacgacgt
    12541 tgccgggcgc cgcccaggcc tcgatggtga actcgccggt ggtcttgatc atgtcggcga
    12601 gcttcctgct gcccgcggtc gtgccttgcg ccttgccacc ctcgcggaca ttgaggcccc
    12661 agccgccgac ccattcgatc gagccggaca tggtcaggtt ggccgcgggc tcgacgccgc
    12721 tcgtgtcgta gacggtcgtg cccatgccgg tcttgaattc gtacagcgcg atctgggcac
    12781 tctcgtagcg attgccgcct gcggccacgg tgccgtcata gagcgtcagc gccttggaga
    12841 gcctgagcgt gggatcgacc ggcgtgaccg cgatggcgtt cgcgaacgct cgcaccgccg
    12901 tttccatggt ggacgcgtcg ttcaggcagc tcgacgtcca gcagttgtgc gactcttcgc
    12961 gcaggcgcac gacgaggcgc gacagctcgg gcgcatcgag attgatcttc gggcgcgccg
    13021 ccgcgtatgc cgcgtcggca tcctgggcgg caaagtacgg cgcctgcgcg gtggccgagg
    13081 ccggcgtatg gcagcgcagg cactggccct gaccgcgcac cagcggcagg atcgtcgtcg
    13141 cgtagaggct cgaactgtcc gggaaggtct tgctgtcgcc gacttccctg atcggcggtt
    13201 cctgcagctg gatggtcttg ccgccgccgg cgagcgagcc cgcccagttg cggatccaca
    13261 ccgtcatcgt gtcgccgcag gccgatgccg aggctagcca gcagttgtgg ccgccggcga
    13321 ccttggtcac gaggcgggac tggtcgggct gggtcaggtc gacgagcgcc accgccgcct
    13381 ggtaggcgag attcacgtcg tcgttgcgcg cgaaggtcgg cgactggccg cccgcgttgt
    13441 ggcagctgcc gcagcggttg ctcgccttga tgttctccca caggttgatg cggaacgcct
    13501 gcacgtcggc attggcaggc gccgggccgg tgtagtccgc gaccggaggc ggcgtcgtga
    13561 ccggattgac ttcggtcggc gcaccactgc cactgcaggc ggcaagagcg gcgaggaccg
    13621 ttgccaggag cacgttgcgg ttcatgacgc tcacctcaat ttcccatgca ataggcggcg
    13681 gtttcggcga acacggtctt cagccggtag ccactgttgc gaaagctctg ggcgatcgcg
    13741 gccgcgcggg tacggtcggc cgcgttctgc ggcgcgcgga agcacacggt cttgaacacc
    13801 ttctgcacct ggcagctggc gaaggcctcg ctgttggcga gctcatcgcc cagcgatttc
    13861 gcgccggcgc cggcgcccgt ggtcgtcgtt gtgccccagc cccagccgag cagccggttg
    13921 tggccactgc gccagtagtt gtcccagctg tcatcgggcg tcacgtagcc gggcttgaag
    13981 ttgtccgcgt tggcgaagta cttcgcgttc ggcgtgcccg gcgtgtacac gagccgctgc
    14041 tgggtctcgt cgaagtcgta gtaggcgaac gcctgcgtca tcgggtccat gccggtgtgg
    14101 cagctgatgc agttgttcag gtacacgcga ctgtcgccgc ccgggctgcg gctcacgtcc
    14161 tggcggatgc gatccggcgg cagcgtcgga tcctgcacct gctcgagatc gcggcacagg
    14221 tggttcatca gcgtgaagcg gaacatcgcg cggttggtgc cgtcgatgaa gaacgcctcc
    14281 gaggaagcgc gcgtcgtgat caggccggcg gtcgccgcgg cc
//
LOCUS       DAOQVY010000056        13924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_4951_length_13924_cov_4.827890, whole genome shotgun sequence.
ACCESSION   DAOQVY010000056 DAOQVY010000000
VERSION     DAOQVY010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13924
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4951_length_13924_cov_4.827890"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..707
                     /gene="phaR"
                     /locus_tag="PKN91_06830"
     CDS             198..707
                     /gene="phaR"
                     /locus_tag="PKN91_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HNS27821.1"
                     /translation="MSEPRVIKKYPNRRLYDTVESRYITLADIRRLVTERVDFVVIDK
                     KSQTDITRAILLQVIAEQEHGSEPVLSRDFLSQVIRSYGGQLQGVVASYLEQTLRLFT
                     AQQREMRERIRQLAGPEATEAVSQLAQKNYQRWRTLQDEIFRTLFGNQNRARERDDEE
                     RVHDPGPRP"
     gene            complement(732..2816)
                     /gene="pnp"
                     /locus_tag="PKN91_06835"
     CDS             complement(732..2816)
                     /gene="pnp"
                     /locus_tag="PKN91_06835"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017391359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNS27822.1"
                     /translation="MSAFKKSFVFGAHTVTLETGELARQANGAVVVSMGDTKVLVTAV
                     AAKEAAKGRDFFPLTVNYVEKTYAAGRIPGGFFRREGRPSEKETLTSRLIDRPIRPLF
                     PDGFYNDVQVVSMVLSLDPEIDADIPSLLGASAALAIAGVPFKGPIGAARVGYRDGKY
                     LLNPTAKELASSQLHLVVAGTQHAVLMVESQADCLSEEVMLGAVVFGHTEMQVAINAI
                     NELVREAGKPRWDWQPATDSAELAALVKDAAEAKLREAYTITEKQSRYARIGEVKAEV
                     TAALTGGETPKWTADQVDDLLFKLESDIVRKRIIAGEPRIDGRDAVTVRPITAKVGVL
                     PRTHGSGQFTRGETQAMVVTTLGTTRDAQIIDALEGERREPFMFHYNFPPFSVGETGM
                     MGSPKRREIGHGNLARRGVAAVMPDMTTFPYTIRVVSEILESNGSSSMASVCGASLSL
                     MDAGVPIKAPVAGVAMGLVLEGDKYKVITDILGDEDHLGDMDFKVAGTKDGVTALQMD
                     IKVEGVTPEIMKVALAQAHAGRLHILGEMAKVISAPRATMSEYAPSIITIRIDPEKIR
                     DVIGKGGAVIRQITEETGTTIDIENDGSVKIASVNGEAGREAQRRIELITADVEVGRV
                     YEGKVARLMDFGAFVTILPGRDGLVHISQISEERVERVSDKLREGDNVRVKVLEVDRQ
                     GRVRLSMRNIDA"
     gene            complement(2898..3167)
                     /gene="rpsO"
                     /locus_tag="PKN91_06840"
     CDS             complement(2898..3167)
                     /gene="rpsO"
                     /locus_tag="PKN91_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNS27823.1"
                     /translation="MSLSTEKKSGVLAQFRRDPKDTGSPEVQVALLSERINGLGEHFS
                     AHKRDHSSRRGLVKLVNQRRKLLDYLKATKPEKYQEVVERLGLRR"
     gene            complement(3242..4135)
                     /gene="truB"
                     /locus_tag="PKN91_06845"
     CDS             complement(3242..4135)
                     /gene="truB"
                     /locus_tag="PKN91_06845"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNS27824.1"
                     /translation="MPSGILLLDKPRGLSSNAALQRVKRAYGAAKAGHVGSLDPLATG
                     MLPICLDEATKVAGEIVAGAKRYEFTVALGTRTATGDVEGEVVESQPVPTLDSMRVES
                     VLASLHGEQQQVPPMFSALKRDGRPLYELARRGIEVERAARGITIHRLALDDLREGAL
                     DLAVECSKGTYVRTLAEDVARALGTCGHVTRLHRAWVAPFEGERMHALEAVEAACAAG
                     APVPLLPIDRGLPDWPAIELSAAAVARARQGQAIGRADLPRGAAAPGAMVRMLDPTGR
                     LLGLGEVDGFGGLRPRRIFNL"
     gene            complement(4142..4552)
                     /gene="rbfA"
                     /locus_tag="PKN91_06850"
     CDS             complement(4142..4552)
                     /gene="rbfA"
                     /locus_tag="PKN91_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNS27825.1"
                     /translation="MAGNARRERIEGEVQRVLSTLVAREVKDPRVGSVTITAVQLAPD
                     LSVARVFFLPFAGRHEAEEVRAGLASAAGFLRGEVGRRLSLRHAPRLEFVIDRHLEAA
                     SHLTELIDRAIDEDRTRAGSDAPAETESPADPSR"
     gene            complement(4567..7113)
                     /gene="infB"
                     /locus_tag="PKN91_06855"
     CDS             complement(4567..7113)
                     /gene="infB"
                     /locus_tag="PKN91_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNS27826.1"
                     /translation="MAEVTVTQFAEVLKVPVDRLLVQLDQAGIQVSGPDDRISDEAKL
                     ELLTHLRRSHGQGEEVATSPRKITLKRKSQSEIKLPSAQGRARTVNVEVRRKATYIKR
                     DVLQEQARQQQEELDSQKREADESLRIEQERAEAARLEHERIEAENRRRIEEEQNRKR
                     AQDDARRLAEENARAEAERERERKAQAEHKAPEAPPPPKAERVRAATPAQGAQQPTRY
                     GRDQLHVAGDVSSRMKKRKRALPSRSRQSVAAGQEARHGFEMPTAPVRREVAVGETIT
                     VAELAQKMAVKATEVIKVMMNMGVMATINQPIDQDTAILVVEEMGHTAKAQKSDELDA
                     DLQGAEAGSGELLPRPPVVTVMGHVDHGKTSLLDYIRRTKVVAGEAGGITQHIGAYHV
                     ETDKGVITFLDTPGHAAFTAMRARGAKATDVVVLVVAADDGVMPQTIEAIQHARAAEV
                     PIVVAVNKIDKSEADPDRVRTELSKYEVIPEDWGGQNMFVHVSARAGTGIEQLLDAIL
                     LQAEVLELKAPQTGLASGVVIESAIEKGRGAVATVLVKKGTLRTGDPIIAGQEFGRVR
                     AMLDETGKQVQEAGPSMPVVVLGLSGAPNAGDDVLAVESERKAREVALYRQGKFRDVK
                     LARQATRAEDVFSQMGGEKAGTVAVVLKTDVQGSAEALRESLTKLSTDEVKVNVVASG
                     VGGITASDVQLAAASRALIIGFNVRADSAAREALKDTGIDVRYYSVIYEAIDDVKQMM
                     SGMLAPEIKEQIVGVAEVREVFRSSKFGVVAGCLVTEGFVKRNNPIRVLRDNVVIFEG
                     ALESLKRFKDDASEVRAGTECGIGVKNYQDVRVGDQIECFSRVEVARTIA"
     gene            complement(7132..8628)
                     /gene="nusA"
                     /locus_tag="PKN91_06860"
     CDS             complement(7132..8628)
                     /gene="nusA"
                     /locus_tag="PKN91_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNS27827.1"
                     /translation="MNKEILMVVDAVSNEKGVDKEVIFEALEAALAAATRKKHGEEWD
                     VRVAIDRKSGDYDTFRRWKVFADDSTELEVPDRELRLEDARDMDPKAEVGGYVEEPME
                     SVAFGRIAAQQAKQVIVQKVREAERAQVIDQYKDRVNSLLSGVVKRVDRNGIYIDLGG
                     NAEGFVPRSEMIPREQVKPQDRIKAYLKDVRPEPRGPQLFLSRTAPEFLIELFKLEVP
                     EVGQGLIEILGAARDGGVRAKIAVRSNDPRIDPVGACVGMRGSRVQAVSNEIAGERVD
                     IIPYNENPAQFVINAMSPAEVLSIVMDEDAHSMDIAVAEDKLSQAIGRGGQNIRLASQ
                     LTGWDLNVMTQSDAEAKSETESRALVENFMKQLDVDEDVALILVQEGFSSVEEIAYVP
                     QAEIASIEEFDEDMIRELRNRARDVLLTQAIASEESLDQQMPAEDLLLLEGMQPDLAL
                     ALARRGVRTREDLADQAVDDILDIEGLSPEEAGKLIMKAREPWFEASH"
     gene            complement(8658..9086)
                     /gene="rimP"
                     /locus_tag="PKN91_06865"
     CDS             complement(8658..9086)
                     /gene="rimP"
                     /locus_tag="PKN91_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNS27828.1"
                     /translation="MGLLEPLVEQLGFELVDVEANAGRGSGLLRLYIDSPAGVTVDDC
                     ERVSREVSALLDVEDPMPGAFTLEVSSPGFDRVLRKPAHYARFRGERVRVELAEARDG
                     RRRYTGLLVAVGEDGIELEVDGEIVAMNFAAIDRARLAPL"
     gene            complement(9393..10805)
                     /locus_tag="PKN91_06870"
     CDS             complement(9393..10805)
                     /locus_tag="PKN91_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNS27829.1"
                     /translation="MAFWNRRTSIDVLDHIDARHRLKRTLRWPHLVALGVGAIVGTGI
                     YTLIGVGAGRAGPAVILSFLIAGIVCACAALAYAELATMMPAAGSAYTYTYAVGGELM
                     AWVVGWSLILEYSVVCSAVAVGWSGYAAGFLQSIGVDLPYWLLAGPHAGGVFNLPAAV
                     ILFAVAALLMVGTRESATVNAVLVVLKLTALLVFIVLAAKAFNGEHFTPFMPYGFQAH
                     LDGTEVRGVMAAAAIIFFAFYGFDAVSTAAEETIDPKRDLVRGIIGSMVVCTLLYMVV
                     AAAALGASYYTDFAASPEPLAYILRSLDHPVAANLIAAAAVIALPTVILAFMYGQSRI
                     FFVMARDGLLPQRLGTVSARSGTPVAMILVTAIVTALIGGIFPLAEIAELANAGTLCA
                     FVAVSLCVLALRVREPDRPRVFSTPWPWLVAPLAFVGCVYLFISLPAATRNRFFIWNA
                     IGIVVYLLYGRLKSRLATAR"
     assembly_gap    10861..10870
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10979..12661
                     /locus_tag="PKN91_06875"
     CDS             10979..12661
                     /locus_tag="PKN91_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27830.1"
                     /translation="MKRSVLIVIAVLLAAAGGFWLIRSQQGATAPPLPDSGPVGATLL
                     EGLGNYSMPVTSDHPEVQRWFDQGLMLTYGFNHDAAERSFLKAAELDPECAMCWWGAA
                     LVLGPHVNAGMDPANVPLAMERIRRAQQATGASERERAYIEALSTRYAAEPGDDRRPL
                     DEAYAAAMAQLVAQYPDDLDAATMHVEALMDLQPWDYWDAEGNPRGNSAEIVSRLEAV
                     IARNPDHAGALHLYVHAVEASNDPARGVAAADHLRTLIPGSGHLVHMPAHIYARVGRY
                     HDAVLANQVAIAADDAYLATCRPRPGVYPLGYVPHNHHFMWFAATMEGASAIAVDAAK
                     ATGERTSDPQLMRTPGFEAMQVFSLTPLFAAVRFGHWDDVAAMPRPADDLPYMVAMWH
                     YAQGTAAANQGRLDDARTHHEPLRAAASDAGIEQMKVWDRYSVINGVRIGERLLAAEI
                     ARAEKDYAAAASALREAVVYEDEIPYDEPPGWHAPVRQKLGAVLLQAGDAAAAEATFR
                     EELRRNPENGWSLFGLEQSLRAQGRAKEADEVAGRLELAWANADVELSVAAL"
     gene            12873..13292
                     /locus_tag="PKN91_06880"
     CDS             12873..13292
                     /locus_tag="PKN91_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27831.1"
                     /translation="MPEREPPPTGDQSDKRRHGAPFSLRLTPEERKALDALADGQPLG
                     AYIKDALLNREVRPRTRGAKPVKDRAALAKLLGLLGQSRLSQNVNQLAKAANTGSLPL
                     NEEVHKALLESCGAIHSMRDLLLTALGLTPGGTKEDG"
     gene            13289..>13924
                     /locus_tag="PKN91_06885"
     CDS             13289..>13924
                     /locus_tag="PKN91_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27832.1"
                     /translation="MILKGNQRGGGRQMAAHLLNGMQNEHVTIHQVRGFLATNVRGAL
                     EEAYALARGTQCQKYLYSLSLNPPKDASVPIEAFEKALKTIEEKLGLTDQPRVVVFHE
                     KEGRRHAHCVWSRIKVDEMKAVNIAYDRTKLQAIARQLFIEHGWTLPRGLMDKAHRNP
                     LTYTREEWQQAARTGRSAAAIKSCLQECWAAADGAKAFAAVLQERGFLLAHG"
BASE COUNT         2253 a         4871 c         4512 g         2278 t
ORIGIN      
        1 tcctcgggct cgaaccgccg gcagacctgg acggtgcccc ggtgggcgat ctgtgggcct
       61 gggcgctgcc gcgctgggca cccgagcgcc tgcgcggcgt gcggacactg cccgcaagcg
      121 aaattcttgg gggttgcagc ccggttgggc tatcgtagtg ccgtacgact gggggcggac
      181 gaggcgagga catgcccatg agcgaaccca gagtcatcaa gaagtacccc aatcgccggc
      241 tctacgacac cgtcgagagt cgctacatca cactggcgga catcagacgg ctcgtgaccg
      301 agcgcgtgga tttcgtcgtg atcgacaaga agtcgcagac cgacatcacg cgggcgatcc
      361 tgctgcaggt gatcgccgag caggagcacg gcagcgagcc ggtgctcagc cgcgacttcc
      421 tctcgcaggt gatccgcagc tatggcgggc aattgcaggg cgtcgtcgcc agttacctcg
      481 agcagaccct gcgcctgttc acggcgcaac agcgcgagat gcgcgagcgc atacgccagc
      541 tcgccggccc cgaggccacc gaggctgtct cacagctcgc gcagaagaac taccagcgct
      601 ggcgcacgct gcaggacgag atattccgca cgctgttcgg caaccagaac cgcgcgcggg
      661 aacgtgacga cgaagaacga gtgcacgacc ctggcccgcg gccctgagcc gcaggccccc
      721 gggccagctg ttcaggcatc gatgttgcgc atcgacaggc gcacgcgacc ctggcggtcc
      781 acctcgagca ccttcacgcg cacgttgtcg ccttccctga gcttgtccga gacccgctcg
      841 acccgctcct cggaaatctg cgagatgtgc acgaggccgt cgcgacccgg caggatcgtc
      901 acgaacgcgc cgaaatccat caggcgcgcg accttgcctt cgtagacgcg gccgacttcg
      961 acgtcggcgg tgatcagctc gatgcggcgc tgggcttcgc ggcccgcctc gccgttgacc
     1021 gaggcgatct tcacggaacc gtcgttctcg atgtcgatcg tcgtgccggt ctcctcggtg
     1081 atctggcgga tcaccgcacc gcccttgccg atgacgtcgc ggatcttctc ggggtcgatc
     1141 ctgatcgtga tgatcgacgg cgcgtactcc gacatcgtcg cgcgcggcgc ggagatcacc
     1201 ttcgccatct cgccgagaat gtggaggcgg ccggcgtgcg cctgcgccag cgcgaccttc
     1261 atgatctcgg gcgtcacgcc ctcgaccttg atatccatct gcagcgcggt gacaccgtcc
     1321 ttcgtgcccg cgaccttgaa atccatgtca ccgaggtggt cctcgtcgcc gaggatatcg
     1381 gtgatgacct tgtacttgtc gccctcgagc acgagaccca tcgcgacgcc ggccaccggc
     1441 gccttgatcg gcacgccggc gtccatcagc gagaggctcg cgccgcacac gctcgccatc
     1501 gacgaggagc cgttcgactc gaggatctcg gagaccacgc ggatcgtgta cgggaaggtc
     1561 gtcatgtcgg gcatcaccgc cgcgacgccg cggcgcgcga ggttgccgtg gccgatctcg
     1621 cggcgcttcg gcgagcccat catgccggtc tcgccgaccg agaacggcgg gaagttgtag
     1681 tggaacatga acggctcgcg gcgttcgccc tcgagcgcgt cgatgatctg cgcgtcgcgc
     1741 gtcgtaccga gcgtcgtgac gaccatcgcc tgggtctcgc cgcgcgtgaa ctggcccgat
     1801 ccgtgggtac gcggcagtac gccgaccttg gcggtgatcg ggcgcacggt cacggcgtcg
     1861 cggccgtcga tgcgcggctc gccggcgatg atgcgcttcc tgacgatgtc gctttcgagc
     1921 ttgaacagca ggtcatcgac ctggtcggcg gtccacttcg gcgtctcgcc gccggtcagc
     1981 gccgcggtga cttcggcctt cacctcaccg atgcgcgcat aacgcgactg cttctcggtg
     2041 atcgtgtacg cctcgcgcag cttcgcctcg gcggcatcct tcacgagcgc ggcgagttcg
     2101 gcactgtcgg tggctggctg ccagtcccaa cgcggcttgc cggcctccct gacgagttcg
     2161 ttgatcgcgt tgatcgcgac ctgcatctcc gtgtggccga ataccaccgc gccgagcatg
     2221 acctcttccg acaggcagtc cgcctgcgac tcgaccatca gcaccgcgtg ctgcgtgccg
     2281 gcgacgacga ggtgcagttg cgagctggcg agttccttcg cggtcgggtt cagcaggtac
     2341 ttgccgtccc tgtagccgac gcgcgcggcg ccgatcgggc ccttgaacgg cacgcccgcg
     2401 atcgccagcg cggcggaggc accgagcagc gacggaatgt ccgcatcgat ctcgggatcg
     2461 agcgacagca ccatcgagac gacctgcacg tcgttgtaga agccgtccgg gaacagcgga
     2521 cgaatcggac gatcgatcag gcgcgaggtg agcgtttcct tctcggaagg tcgaccctcg
     2581 cgacggaaga agccgccggg gatgcgtccc gccgcgtagg tcttctccac gtagttgacg
     2641 gtcagcggga agaagtcgcg acccttggcc gcttccttcg ccgcaacggc ggtgaccagc
     2701 accttcgtgt cgcccatcga gacgacgacg gcgccgttcg cctggcgagc cagctcgccg
     2761 gtttccagcg tcacggtatg tgcgccgaat acaaaagatt ttttgaaagc gctcacgctt
     2821 caaccctcgc gaaatgagat gtgtgggaaa gcagaacggc cgcgcagctg gactgacgcg
     2881 gccattcggc ggggtcctta gcgacggagc cccaggcgct cgacgacctc ctgatatttc
     2941 tccggcttgg tcgccttgag ataatcgagg agcttgcggc gctggttcac gagcttgacg
     3001 agcccgcgac gcgaggaatg atcacgcttg tgtgcgctga agtgctcgcc gaggccattg
     3061 atgcgttcgg aaagcagcgc aacctgcact tcgggcgatc cggtgtcttt cggatcgcgg
     3121 cggaactgcg cgaggactcc gctctttttt tccgtggata gcgacattac agagaaatcc
     3181 ttgaaaatct tggctttgta ccgttttaga ggcgcggcat tctaaccgca cgcctgtccg
     3241 ctcacaagtt gaagatccgc cgcggccgca gcccgccgaa cccgtccact tcaccgaggc
     3301 cgaggagccg gcccgtgggg tcgagcatcc gcaccatcgc cccgggggcg gctgcgccgc
     3361 gcggcaggtc agcccgcccg atcgcctgcc cctgtcgtgc gcgcgccacg gcggccgccg
     3421 acagttcgat cgcgggccag tccggcaaac cccgatcgat gggcagtaac ggcacgggcg
     3481 cgccggccgc acaagcggcc tccaccgcct cgagcgcgtg catcctctcg ccctcgaaag
     3541 gtgcgaccca ggcgcgatgc aggcgggtga catgcccgca ggtcccgagg gcgcgcgcaa
     3601 cgtcctcggc aagggtgcgc acataggtgc ccttcgagca ttcgaccgcg agatcgaggg
     3661 cgccttcccg caggtcgtcg agtgcaaggc gatgaatcgt gatgcctcgc gctgcgcgtt
     3721 cgacctcgat gccgcgcctc gcgagctcat agagcggacg gccgtcgcgt ttgagtgccg
     3781 agaacatggg cggtacctgc tgctgctcgc catgcaggct ggcgagcacg ctctcgacgc
     3841 gcatgctgtc gagtgtcggc accggctgcg attcgaccac ttcgccttcc acgtcaccgg
     3901 tcgccgtgcg cgtgccgagc gcgaccgtga actcgtagcg cttggcgccg gccacgatct
     3961 cgccggccac tttggtggct tcatccaggc agatgggcag catgccggtt gcgagcggat
     4021 cgaggctgcc gacgtgtcca gccttggcgg cgccataggc ccgtttcacg cgctgcaatg
     4081 cggcgttcga cgacaggccc cgcggcttgt cgaggagcag gattccggag ggcatcggcg
     4141 ctcagcggct cggatcggcg ggtgattcgg tctcggcggg cgcgtcggac ccggcccggg
     4201 tgcgatcctc gtcgatcgcg cggtcgatca gttcggtgag gtgcgaagcc gcctccaggt
     4261 ggcgatcgat cacgaactcg agccgcggcg catgacgcag cgacagccgc cggcccacct
     4321 cgccgcgcag gaaacccgcc gcgctcgcca gtcccgcacg cacttcttcg gcctcgtggc
     4381 gcccggcgaa aggcaggaag aacacgcgcg cgaccgacag gtcgggcgca agctgcacgg
     4441 cggtgatcgt cacgctgccg acacgcgggt ccttgacctc gcgcgccacc agcgtcgaga
     4501 gcacacgctg cacctcgccc tcgatgcgct cgcggcgtgc gttaccggcc atggttcacg
     4561 cgcccatcac gcgatggtgc gcgcaacctc caccctcgag aagcactcga tctggtcgcc
     4621 gacgcgcacg tcctggtagt tcttcacgcc gatgccgcat tccgtgccgg cgcgcacttc
     4681 gctcgcatcg tccttgaagc gcttcagcga ctcgagcgca ccctcgaaga tcaccacgtt
     4741 gtcgcgcaat acccggatcg ggttgttgcg cttcacgaag ccttccgtca ccaggcagcc
     4801 ggcgaccacg ccgaacttgc tcgagcggaa tacctcgcgc acctcggcca cgccgacgat
     4861 ctgttccttg atctcgggcg cgagcatgcc cgacatcatc tgcttcacgt cgtcgatggc
     4921 ctcgtagatg acgctgtagt agcgcacatc gataccggtg tccttgagcg cctcgcgcgc
     4981 ggccgagtcg gcacgcacgt tgaagccgat gatcagcgcg cgcgacgcgg ccgccagctg
     5041 cacgtcggac gcggtaatgc cgccgacgcc gctcgcgacc acattgacct tcacctcgtc
     5101 ggtcgacagc ttggtcagcg attcccgcag cgcctcggcg ctgccctgca cgtcggtctt
     5161 cagcaccacg gccaccgtgc cggccttctc cccgcccatc tgcgagaata cgtcctcggc
     5221 gcgcgtcgcc tggcgggcga gcttgacgtc gcggaacttg ccctggcgat agagcgcgac
     5281 ctcgcgtgcc ttgcgctcgc tctccaccgc gagcacgtcg tcgcccgcgt tcggcgcgcc
     5341 tgacaggccc agcacgacca ccggcatcga tggaccggcc tcctgcacct gcttgccggt
     5401 ctcatcgagc atggcgcgca cgcggccgaa ctcctggccc gcgatgatcg gatcaccggt
     5461 gcgcagcgtg cccttcttga cgagcacggt cgcgaccgcg ccgcggccct tctcgatcgc
     5521 cgattcgatg accacgcccg aggcaaggcc ggtctgcggc gccttgagct ccagcacttc
     5581 ggcctgcagc aggatcgcgt cgagcagctg ctcgatgccg gtgccggcgc gcgccgacac
     5641 atgcacgaac atgttctggc cgccccagtc ctccggaatc acctcgtact tggacagctc
     5701 ggtacgcacg cggtccggat ccgcttcgct cttgtcgatc ttgttcaccg ccacgacgat
     5761 cggcacctcg gcggcacgcg cgtgctggat cgcctcgatc gtctgcggca tgacgccgtc
     5821 gtcggcggcg acgaccagca ccacgacgtc ggtcgccttc gcgccgcggg cgcgcatcgc
     5881 ggtgaacgcc gcgtggcccg gggtatcgag gaaggtgatc acgcccttgt cggtctcgac
     5941 gtggtacgcg ccgatgtgct gggtgatgcc gccggcctcg cccgccacga ccttggtgcg
     6001 gcggatgtaa tcgagcagcg aggtcttgcc gtggtcgacg tggcccatga cggtgaccac
     6061 cggcgggcgc ggcagcagtt ccccggaacc cgcctcggcg ccctgcaggt cggcatcgag
     6121 ctcgtcgctc ttctgcgcct tcgcggtgtg gcccatctcc tcgaccacca ggatcgccgt
     6181 gtcctggtcg atcggctggt tgatcgtcgc catgaccccc atgttcatca tcaccttgat
     6241 gacttcggtg gccttgaccg ccatcttctg cgcgagctcg gcgaccgtga tcgtctcgcc
     6301 gaccgccacc tcgcgacgca ccggcgcggt cggcatctcg aagccgtggc gcgcctcctg
     6361 tccggcggcg accgactggc gcgaacgcga cggcagcgcg cgcttgcgct tcttcatgcg
     6421 cgaactgacg tcgccggcga cgtgcaactg gtcgcggccg tagcgggtcg gctgctgggc
     6481 gccctgcgcc ggcgtcgcgg cgcgaacgcg ctcggccttc ggcggcggtg gcgcctcggg
     6541 cgccttgtgc tcggcctggg ccttgcgttc gcgctcgcgc tcggcttcgg cgcgcgcatt
     6601 ctcctcggca aggcggcgtg cgtcgtcctg cgcgcgcttt ctgttctgct cttcttcgat
     6661 gcgccggcgg ttctccgcct cgatgcgctc gtgctcgagt cgcgccgcct cggcgcgctc
     6721 ctgctcgatg cgcagcgatt cgtcggcctc gcgcttctgg ctgtcgagct cctcctgctg
     6781 ctggcgcgcc tgctcctgca gcacatcgcg cttgatgtag gtcgccttgc ggcgcacttc
     6841 gacattgacg gtgcgcgcgc gcccctgtgc gctcggcagc ttgatctcgc tctgcgactt
     6901 gcgcttcagc gtgatcttgc gcggcgaggt cgcgacttct tcgccctgcc cgtggctgcg
     6961 gcgcaggtgc gtcagcaact cgagcttggc ctcgtcgctg atccggtcgt ccggaccgga
     7021 cacctggatg cctgcctggt cgagctgcac gagcagccgg tcgacgggca ccttgagcac
     7081 ttcggcaaac tgggtgacgg tcacttcagc catgggtggt taccttcatc cttagtggct
     7141 cgcctcgaac cagggttcgc gcgccttcat gatgagcttg ccggcttctt ccggcgacag
     7201 cccttcgata tcgaggatgt cgtcgaccgc ctggtcggcg agatcctcgc gcgtgcgaac
     7261 gccacggcgg gcgagcgcga gtgcgaggtc cggctgcatg ccctcgagca gcagcaggtc
     7321 ttcggcaggc atctgctggt ccaacgattc ctccgaggcg atcgcctggg tcagcagcac
     7381 gtcgcgcgcg cgattgcgca gctcgcggat catgtcctcg tcgaactcct cgatcgaggc
     7441 gatctcggcc tgcggaacgt aggcgatctc ctcgaccgac gagaaacctt cctgcaccag
     7501 gatcagcgcc acgtcctcgt cgacgtcgag ctgcttcatg aagttctcga ccagcgcgcg
     7561 cgattcggtc tcgctcttcg cctcggcatc cgactgcgtc atgacgttga ggtcccagcc
     7621 ggtcagttgg ctcgcgagac ggatgttctg cccgccgcgg ccgatagcct gcgacagctt
     7681 gtcctccgcg actgcgatat ccatgctgtg cgcgtcctcg tccatcacga tcgacagcac
     7741 ttcggccggc gacatcgcgt tgatgacgaa ctgggcggga ttctcgttgt acgggatgat
     7801 gtcgacacgc tcgccggcga tttcgttcga cacagcctgc acgcgcgagc cgcgcatgcc
     7861 gacgcaggcg ccgaccggat cgatgcgcgg atcgttgctg cgcacggcga tcttggcgcg
     7921 cacgccaccg tcgcgcgcgg cgccgaggat ctcgatcagg ccctggccta cttccggcac
     7981 ctcgagcttg aacagctcga tcaggaattc cggcgcggtg cgcgacagga acagctgcgg
     8041 accgcgcggc tcggggcgca cgtccttcag ataggccttg atgcgatcct ggggcttgac
     8101 ctgctcgcgc gggatcattt cgctgcgggg cacgaagccc tcggcattgc cgccaaggtc
     8161 gatgtagatg ccgttacggt cgacgcgctt gacgactccc gagagcagcg agttcacgcg
     8221 gtccttgtac tggtcgatga cctgggcacg ctcggcctcg cgcaccttct gcacgatgac
     8281 ctgcttggcc tgctgggccg cgatgcggcc gaacgccacc gactccatcg gctcctcgac
     8341 gtaaccgccg acttcggcct tcggatccat gtcgcgcgcg tcttcgagcc gcagttcgcg
     8401 gtccggcact tccagctcgg tggagtcgtc ggcgaacact ttccagcggc ggaaggtgtc
     8461 gtaatcgccg ctcttgcggt cgatcgcgac gcgcacgtcc cactcctcgc cgtgcttctt
     8521 gcgcgtagcg gccgccagcg ccgcctccag cgcctcgaag atcacttcct tgtcgacgcc
     8581 cttctcgttc gagacggcat caacgaccat caatatttcc ttgttcatcc cgactcctcc
     8641 aacacgccgc gccgctgtta cagcggcgcg aggcgcgcgc ggtcaattgc cgcaaaattc
     8701 atcgccacga tctcaccatc gacctcgagc tctatgccgt cctcgccgac cgccaccagc
     8761 aggccggtgt agcgccggcg gccgtcgcgc gcctcggcga gttccacccg cacacgctcg
     8821 ccgcgaaagc gcgcgtaatg cgccggcttg cgcagcacgc ggtcgaagcc gggcgaggag
     8881 acctcgagcg tgaacgcacc cggcatcggg tcttccacgt cgagcagcgc cgaaacttcg
     8941 cgactcacgc gctcgcaatc gtcgacggtc acgcccgccg ggctgtcgat gtagaggcgc
     9001 agcagccccg aaccccgacc cgcattcgcc tcgacgtcga cgagttcgaa cccgagctgt
     9061 tcgaccagcg gctcgagcag cccgataagc cgatcgcgta gcgctgtcgc cgtcaccgaa
     9121 cctccgttgc cgcccgcggc gaaaaaaaat gggcccgaag gcccacccaa acctcataca
     9181 ggccacggcc gtacgccgcg cccgggaccc cggccaggga tccatgaaca aaaaaagccc
     9241 ctcggagggg ctttccaaat caataacttg gcacccaaag ctccagtgga gccttcgagg
     9301 agcaggccgc tatctgcgcc gaccggcccc accgagggcg cgaattgtat ccgccccggg
     9361 gcccccggta aacccgtccg cgccagtgcc ggtcagcgcg cggtggcgag ccggctcttc
     9421 agccggccgt acagcaggta gacgacgatg ccgattgcgt tccagataaa gaagcggttg
     9481 cgcgtggcgg ccggcaggct gatgaacagg tacacgcaac ccacgaacgc gaggggcgca
     9541 accagccacg gccacggcgt tgagaacacg cgcgggcggt cgggttcgcg cacgcgcagc
     9601 gccagcacgc acagcgagac cgcgacgaac gcacacagcg ttccggcatt ggcgagttcc
     9661 gcgatctcgg ccagcgggaa gatgccgccg atcagcgcgg tgacgatcgc cgtcacgagg
     9721 atcatggcga ccggcgtgcc gctgcgcgca ctgaccgtgc caagccgctg cggcaacagg
     9781 ccatcgcgcg ccatcacgaa gaagatgcgg ctctggccat acatgaaggc gaggatcacg
     9841 gtcggcagcg cgatcaccgc cgctgcggcg atcaggttcg ccgcgaccgg atggtccagc
     9901 gagcgcagga tgtaggccag cggttccggg ctcgccgcga agtcggtgta gtacgaggcg
     9961 ccgagcgcgg ccgccgcgac gaccatgtag agcagcgtgc agacgaccat cgagccgatg
    10021 atgccgcgca cgaggtcgcg cttcgggtcg atcgtttcct ccgctgcggt cgacaccgca
    10081 tcgaacccgt agaaggcgaa aaaaatgatc gccgccgccg ccatcacgcc gcgcacctcg
    10141 gtgccatcga gatgggcctg gaatccgtag ggcatgaagg gtgtgaaatg ctcgccattg
    10201 aacgccttcg ctgcgagcac gatgaatacc agcagtgccg tgagcttgag caccacgagc
    10261 acggcgttca cggtggcgct ctcgcgcgta cccaccatca gcagcgccgc cactgcgaac
    10321 aggatcacgg ccgcgggcag attgaagacg ccaccggcat ggggaccggc cagcagccag
    10381 tacggcaggt cgacgccgat cgactgcagg aaacccgcgg cgtatcccga ccagccgacc
    10441 gccacggcgc tgcagaccac cgaatattcg aggatcaggc tccagccgac gacccacgcc
    10501 atcagctcgc cgcccacggc gtaggtatag gtgtaggcgc tgcccgccgc gggcatcatc
    10561 gtcgcaagct ccgcgtaggc cagtgccgcg cacgcgcaca cgatgccggc gatcaggaac
    10621 gagaggatca ccgccggtcc ggcgcgcccc gcccccaccc cgatcagcgt gtagatgccg
    10681 gtaccgacga tggctccgac gccgagcgcc acgagatgcg gccagcgcag cgtgcgcttg
    10741 agccgatgcc ttgcgtcgat gtggtcgagt acatcgatcg acgtgcgccg attccagaat
    10801 gccatgcgtc gccccgccca ctctgatgcg gcatcgtagc attccgctgc agtgcgtgat
    10861 nnnnnnnnnn ccgtcagggt ttacattcgg cgtcgaatga attgaaatcc gcccccgttc
    10921 ggccagggtg tggccagcga ccgctgcgtg cggtcaatgc aaaaaggaat tttgcgccat
    10981 gaaacgttcg gtattgatcg tgatcgcggt gctgctcgcc gctgcgggtg gcttctggct
    11041 catccgctcc cagcagggcg caaccgcacc tccactgcct gacagcggcc ccgtcggcgc
    11101 aaccctgctc gaaggtctcg gcaactacag catgccggtc accagtgacc accccgaagt
    11161 gcagcgctgg ttcgaccagg gcctgatgct cacctatggt ttcaatcacg acgcggcgga
    11221 gcgctcgttc ctgaaggcgg ccgaactcga ccccgaatgc gcgatgtgct ggtggggcgc
    11281 cgcgctcgta ctcgggccgc acgtcaacgc gggcatggac ccggcgaacg tgccgctcgc
    11341 catggagcgg atccggcgcg cgcagcaggc caccggcgcg agcgagcgcg agcgagcgta
    11401 tatcgaggcg ctgtcgacgc gctatgccgc cgagcccggc gacgatcgcc ggccgctcga
    11461 cgaagcgtat gccgcagcga tggcgcaact cgtcgcgcaa tacccggacg atctcgacgc
    11521 cgcgacgatg cacgtcgagg ccctgatgga cctgcagccc tgggactact gggacgccga
    11581 gggcaatccg cgcggcaaca gcgcggaaat cgtctcgcgg ctcgaggcgg tgatcgcgcg
    11641 caatccggac cacgcgggcg cgctgcacct ctacgtccac gccgtcgaag cctcgaacga
    11701 tcccgcgcgc ggcgtggccg cggcggatca cctgcgtacg ctgatcccgg gctcgggcca
    11761 tctcgtgcac atgcccgcac acatctatgc tcgggtgggc cgctaccacg acgcggtgct
    11821 cgccaaccag gtcgccatcg ccgccgacga cgcctatctc gccacctgca ggccgcgccc
    11881 cggtgtctat ccgctcggct acgtgcctca caatcatcat ttcatgtggt tcgccgccac
    11941 gatggagggc gcgagcgcca tcgcggtcga tgccgcgaag gcgacgggcg agcgcaccag
    12001 cgatccgcag ctgatgcgca caccgggctt cgaggcgatg caggtgttca gcctcacgcc
    12061 gctgttcgcc gccgtgcgtt tcggccactg ggatgacgtc gccgccatgc cccgccccgc
    12121 agacgacctg ccgtacatgg tggccatgtg gcactacgcg cagggcaccg ccgcggcgaa
    12181 ccagggccgg ctcgacgatg cccggaccca ccacgaaccc ctgcgcgccg ccgcgagcga
    12241 tgccggcatc gaacagatga aggtctggga tcgctattcg gtcatcaatg gcgtgcgcat
    12301 cggcgagcgc ctgctggcgg cggaaatcgc ccgcgcggag aaggactacg cggcggcggc
    12361 gagtgcgctg cgcgaagccg tcgtgtacga ggatgaaatc ccgtacgacg aacccccggg
    12421 ctggcacgcc ccggtgcgcc agaagctcgg cgccgtgctg ctgcaggccg gcgacgcggc
    12481 cgccgcggag gccacgttcc gcgaggaact gcgccgcaac cccgagaacg gctggtcgct
    12541 gttcgggctc gagcagtcac tgcgggcgca gggccgcgcc aaggaagccg acgaagtagc
    12601 cgggcgcctc gagctcgcgt gggcgaatgc ggatgtggag ctgagtgtcg cggcgctctg
    12661 acgggaggcc gcagcggcgc cgggaagatt ggtagcgggg gctccctacc aacgctatct
    12721 actgctcttc caagcgttcg atatcgctgc cggccctgcc gccctttgta cttcaagcgc
    12781 ggtactgtcc aactcggagg gagttcgcgc ttccagagcc gcgcattcta cgggcgtaac
    12841 cccgcgcacc gcaacccaaa acgggggcgg ccatgcctga acgcgagccc ccacccaccg
    12901 gagaccagtc agacaagcgc cgacacggcg cgccgttctc gctgcgcctc accccggaag
    12961 aacgcaaagc gctcgacgca ctcgccgacg gtcagcccct cggggcctat ataaaggatg
    13021 cgctcctcaa ccgggaggtc cggccccgga cccgtggagc caagcccgtc aaagaccggg
    13081 cagcgcttgc caaacttctc ggactgctcg gccagtcgcg gctttcccag aacgtcaacc
    13141 agctcgccaa ggcggccaac accggctccc tgcccctgaa cgaggaggtt cacaaggcgc
    13201 tgctggaatc ctgcggagcc attcactcca tgcgtgacct gctcctgacg gcgctcggcc
    13261 tcacaccggg cggtacgaag gaggatggat gatcctgaaa gggaaccagc gcggcggcgg
    13321 gcggcagatg gccgcgcatc tcttgaacgg gatgcagaac gagcatgtga ccatccatca
    13381 ggtccggggg tttctcgcga cgaacgtgcg cggcgcgctc gaagaagcct acgcgctggc
    13441 gcggggaacc caatgccaga aatatctcta ctctctttcg ctgaacccgc cgaaggacgc
    13501 gagcgtcccc atcgaagcgt tcgagaaggc gctgaagacg atagaagaga agctcggcct
    13561 gaccgatcag ccacgcgtag tcgtcttcca tgagaaggaa ggccgccgcc atgcgcattg
    13621 cgtctggagc cggatcaagg tcgatgagat gaaggccgtc aacatcgcct acgaccgcac
    13681 caagctccag gcgattgccc ggcaactgtt cattgagcat ggctggacct tgccgcgcgg
    13741 gttgatggac aaggcgcacc gcaaccccct cacctacacg cgggaggaat ggcagcaggc
    13801 ggcgcggacc gggcgcagcg ctgccgcgat caaatcctgc ttgcaggaat gttgggcggc
    13861 ggcggatggc gccaaagcct tcgccgccgt gctccaggaa cgcggctttc tgctggcgca
    13921 cggg
//
LOCUS       DAOQVY010000057        13905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_4958_length_13905_cov_5.152202, whole genome shotgun sequence.
ACCESSION   DAOQVY010000057 DAOQVY010000000
VERSION     DAOQVY010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13905
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4958_length_13905_cov_5.152202"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1123)
                     /locus_tag="PKN91_06890"
     CDS             complement(104..1123)
                     /locus_tag="PKN91_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNS27833.1"
                     /translation="MSHPQYEAKELERDEISLCVVQSRVRAVDVRNLESTRTANLEHM
                     LGLIDAANGWLGPKDLVMFHEFPITGFDARWRREELLKVAIEIPGVETEAIAARAKRY
                     GCYVVFGSYARDPAWPNHILSITTVIDRAGQIVGRHWKARNIKGLFGGAFEIFTTTIF
                     DVLDQYVEMYGADEVVPVTRTPIGNICTTSTQLEPEIIRAFALKGGEIMLRTATGGFD
                     EYDIRANARYNQMYTAIANNAISPGNPGSFDDISSGGSAIYGPDGAQIAKANSPAEGP
                     VLARIPIRAFRARRKLPNIHPSLYRDLYANYVEAFPPNLYAHYQPTDGRDAARYLGDR
                     SLWKK"
     gene            complement(1143..2105)
                     /locus_tag="PKN91_06895"
     CDS             complement(1143..2105)
                     /locus_tag="PKN91_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27834.1"
                     /translation="MSVAQSTTAPTSKLKSWLYRPPFVGLLAFIVVFVVQALGHTVMI
                     VMEDIFGENHVILAAFLVGLAGAALLLVGMRNRNEVASTWYGFWAGTLLWTGWIEFSF
                     VWSAQTLGVPDLMDPRVSGEIQTKGEYLVMMSSVGLLGATLAYFLMNKETKCNMFLWI
                     QRYAHLKTGKPNPAQERNFAAITSLETIYVLWFCYLMLLFIYDESILGDHHPVTYVIF
                     FLMIIWAGYLFLRLMKFWKVTTAVRYGIPTAIIAWNAVEIAGRWHLFTEFWEKPWEYA
                     LEMTLFTLGLAIAAWLAIRTPANMKAQLSREQLAAAAAKGGPQL"
     gene            complement(2284..3672)
                     /locus_tag="PKN91_06900"
     CDS             complement(2284..3672)
                     /locus_tag="PKN91_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNS27835.1"
                     /translation="MIRAPGSPLYWRLFLTVAAAIVLFIALAIVTLALLVSRELEGYV
                     HARQSPLGQQAADVFTRGGHDALRRWLTGGAAGIPGDVTVYVVAPDGTELLGRELPGW
                     SARMMRAIAAHGPDPHPDNYRPTVLAPQLVGANGEVLSLFVMPRNVGPWGSSRTTLAL
                     LALALLVIAAAAWLVVRALTRPISELQLAVRGLASGRIEARVPTRIASRRDELGALAT
                     DFNAMADRLARLLEHREHLLRDVSHELRSPLTRLQAATVLAAHHGGLAPADRERIERE
                     IRRMDALIGDILRYSWLGDATSMARRLVRIDELLRDIARDGELIAAGRGVALRLSAAP
                     GQVVVGDPALLRSAFDNLVRNAIRHAPPGSEVEIAAVTGDAIGIEVRDRGEGVPAEYI
                     ERIFEPWFRVPRAAGADRDSSADDSGSTDGGLGLAIARRVFRLHGGDVAALPRDGGGL
                     TLTVHLPPAQVS"
     gene            complement(3669..4388)
                     /locus_tag="PKN91_06905"
     CDS             complement(3669..4388)
                     /locus_tag="PKN91_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNS27836.1"
                     /translation="MPPSASPRILLVDDDAGLAAMLAEILRLHGFEVDGVARGETALT
                     AIRARPPDLVVLDVMLPGISGFDVLTRLRENSDVPVIMLTARGAESERIAGLTRGADD
                     YLGKPFNPLELVARIRAILKRAPGRGSTTRAAQDELSAGTLRLDLKRFELWAGERLLA
                     VTPAELRVLEVLMRRQGEVLSRAELTEFAMQRPLEPYDRSIDTIMSRLRRKLADAQVD
                     NPRLHGVRGHGYVLDVMAGTP"
     gene            4576..5673
                     /locus_tag="PKN91_06910"
     CDS             4576..5673
                     /locus_tag="PKN91_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HNS27837.1"
                     /translation="MIETPYYLVDKAKLLRNLRMIQQVRERSGARVLLALKCFATWSV
                     FDLMREYMDGTTSSSLYELRLGREKFGGETHAYCVAWADAEIDEVVTYADKIIFNSAG
                     QLERFAARTAGLPRGLRVNPQVSSSSFDLADPARPFSRLGESDPARIEAALPMISGFM
                     IHNNCENADFGLFDRLLAQIESRFAALLERVEWVSLGGGIHFTGEGYPLEAFCERLRA
                     FAARYGVQVYLEPGEAAVTGSTTLEVSVLDTLYNGKHLAIVDSSIEAHMLDLLIYRES
                     ARMEPDAGPHSYMICGKSCLAGDIFGEFRFPQPLAVGDRLSILDAAGYTMVKKNWFNG
                     VRMPAIAVRELDGRVNVRREFTYEDFAASLS"
     gene            5718..6962
                     /locus_tag="PKN91_06915"
     CDS             5718..6962
                     /locus_tag="PKN91_06915"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HNS27838.1"
                     /translation="MKSNVLIIGAGGVAQVVAHKCAQNNRLLGDIHIASRTVSKCQAI
                     IDSVRAKRSLQVDGVLEAHAVDAMDIEAVSALIRSTGSRIVINVGASFVNMSVLSACI
                     ATGAAYIDTAIHEDPLKICETPPWYGNYEWRRREACEQAGITAILGAGFDPGVVNAYA
                     ALARDEYFDRIDSIDIIDINAGSHGRWFATNFDPEINFREFTGQVWSWQNGEWTSNRM
                     FEVKKVWDLPVVGESTAYLTGHDEIHSLSRNLDVPHIRFWMGFGDRYITVFTVLKNLG
                     LLSEQPVTTAEGQQVVPLKVVKAVLPDPSSLAPDYTGKTCIGDLVRGVKEGKEREVFI
                     YNISDHEASFAEVGSQAISYTAGVPAAAAAILIAQGTWDVKKMVNVEELPPRPFLGLL
                     GRMGLPTRIRDEQGDRALDFAA"
     gene            complement(6999..7421)
                     /locus_tag="PKN91_06920"
     CDS             complement(6999..7421)
                     /locus_tag="PKN91_06920"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNS27839.1"
                     /translation="MDDIDRKLIALLRVDARASIASLAKALRVARGTVRNRLTRLEES
                     GVIVGYTLRLKPQVEEQHIRALMTVLVEGNRTESVIRALRGDPAVSALYSTNGRWDIV
                     AELRAESLEAFDRVLARIRLIEGISSTETSLLLSTHKL"
     gene            7574..8578
                     /gene="rocF"
                     /locus_tag="PKN91_06925"
     CDS             7574..8578
                     /gene="rocF"
                     /locus_tag="PKN91_06925"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HNS27840.1"
                     /translation="MVDDKGIGRIQAAGTTAALIGVPTDVGASARGASMGPEALRVAG
                     LREAIVRRGMRVVDRGNLGGPANPCLPPEQGYRHLREVVEWNRRVHEAVYAELAARRL
                     PIVLGGDHCLGIGSISAVARHCREAGRKLRVLWLDAHTDFNTAALSPSGNLHGMPLAC
                     LCGFGPPELIGIGGAVPAIRPEWLRLVGIRSVDAGEARFVDQQALEVFDMRCIDEIGM
                     REAMELALAGIDAETHLHVSFDVDFLDPQIAPGVVTTVQGGPSWREAQLCMEMIADTG
                     RLGSLDVVELNPALDVRNQTAAVAVELIESLFGKSTLMRRSPVVGDHSRRNVTRTCVA
                     "
     gene            complement(8556..8687)
                     /locus_tag="PKN91_06930"
     CDS             complement(8556..8687)
                     /locus_tag="PKN91_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27841.1"
                     /translation="MFAARILPVTIALPAAAVAVAYRDDRLNAQLGDVGTITQRTCG"
     gene            8747..9211
                     /locus_tag="PKN91_06935"
     CDS             8747..9211
                     /locus_tag="PKN91_06935"
                     /inference="COORDINATES: protein motif:HMM:NF021390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HNS27842.1"
                     /translation="MSHSLRAVPVARAGVFAVLAISACGERPAEVEAPAAEAPAAEAS
                     VTSMEPETVREVLYRCNSGREVAARYMAGGGKPSRVELLFDGAAYELHAVPAAEGALY
                     ATDDGRTSGRGLNWHNKGAEAALTETMAGEPAGTGVPVDSCHEQPESHAEVE"
     gene            9195..9947
                     /locus_tag="PKN91_06940"
     CDS             9195..9947
                     /locus_tag="PKN91_06940"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HNS27843.1"
                     /translation="MPKSSDAAPPSNAAQRQSPSYRLAALDQDFILGDSMRGARFMLE
                     YAKAEETLRRWRIQSTIVVFGSARAQENGAGQLGQWYAEARRFGRIASERGGALTSNS
                     AERQNVIATGGGPGIMAAANRGASEVGAPSIGFNITLPQEQEPNPYSTPELTFLFHYF
                     AMRKMHLAMRASALVVFPGGFGTLDELFEILTLQQTHKAPKIPTVLVSRDYWTSVVDF
                     DTLVGHGMIDAADLALFSYAADAEEAWTRLAR"
     gene            9957..10622
                     /locus_tag="PKN91_06945"
     CDS             9957..10622
                     /locus_tag="PKN91_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNS27844.1"
                     /translation="MAHFLIAFPALFSIINPVGGSFVFLSLTRHYPPQVRAQLARWVA
                     IYAFAIIIGSVYIGAYVLGFFGISIPVLRVAGGIVIATAGWRVLNAPDDAPPKGESAA
                     GDSASALAERETVRRMAFFPLAMPLTTGPGTISVAVSLGATRPREFAQLVNFVWQVAL
                     AALCMSAAIYLLFRYSDRVLRALGPTGSVIIQRLSAFLLFCIGVQVFWSGVSELLAQQ
                     AAL"
     gene            complement(10623..11846)
                     /gene="tagH"
                     /locus_tag="PKN91_06950"
     CDS             complement(10623..11846)
                     /gene="tagH"
                     /locus_tag="PKN91_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HNS27845.1"
                     /translation="MALRLRVVSSQRRSLGGRGTIVFGVGGGTIGRAADNDWVLPDPM
                     RYVSSCHARVHFRNGKWLLEDLSTNGAFVNDQDRPIGKHGTHTLANGDIVRIGEYELL
                     VAIDAATDFPPDESAVVALDVLGAGRPDPVATHGDIGASINMASLLSMDVSPSDSFRA
                     VNAFGQAVKTPFDPARDSGIERQADVVARRMERLARAARAQEQAQPAALYDVQTGLAA
                     FCRGAGIDPDRLPADAQTRMLHLAGQMFREALLGLKDLSRHHHEMRNNYRIDLPASAA
                     DEPFPLDRGAVDELVTSMLASHDSRRIDAVQWLRDRFEDTRNHEKAAGAALRAAFIEF
                     IDRLDPAELEGRFERALKKGKAGSVQRGQYWDLYADFYRNITEMPANQLPHVFVEAFA
                     RAYGEARKPRKSDAA"
     gene            11935..12252
                     /locus_tag="PKN91_06955"
     CDS             11935..12252
                     /locus_tag="PKN91_06955"
                     /inference="COORDINATES: protein motif:HMM:NF020426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HNS27846.1"
                     /translation="MFVNQGKVYEIYARKVHHGNLFGFIEIEELVFGERSSVVVDPGE
                     ERVKGEFEGVKRTWLPLHSVLRIDEVKKQGVSKITAFEGGNVAQFPGPIYTPPPGSEG
                     PRK"
     gene            12265..>13905
                     /gene="purL"
                     /locus_tag="PKN91_06960"
                     /gene_synonym="purI"
     CDS             12265..>13905
                     /gene="purL"
                     /locus_tag="PKN91_06960"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNS27847.1"
                     /translation="MLVLKGAPALSPFRLAKLLGRLRSLAPSASAIDAHFIHIVDVAR
                     PLEAAERGLLDSLLTYGPRIVAAAAGGPGVRRLIVVPRPGTVSPWASKATDIARVCGL
                     DAVRRIERGIEYRIAGVGGLDAEALRRLAAVLFDRMTEAVFVDETELAALFVHPEPGV
                     VRTVPRAGGRAALDDANRELGLALSDDEIDYLVEAFERLGRDATDVELMMFAQANSEH
                     CRHKIFNADWIIDGVTQAKSLFAMIRNTHARAPRGVLSAYRDNAAVIEGSIGARWFAD
                     PRGGVYRSSVESIDILMKVETHNHPTAISPFPGAATGAGGEIRDEGATGLGAKPKAGL
                     AGFSVSHLRLPGLPQPWEDAFGKPDRIASALDIMLEGPIGAAAFNNEFGRPNICGYFR
                     NFELRGAGDPASRVRGYHKPIMIAGGVGNVRRDHVEKGEVPVGAKIVVLGGPAMLIGL
                     GGGAASSVGSGASSAALDFASVQRGNPEMQRRAQEVIDRCWAMGAANPILLIHDVGAG
                     GLSNAVPEVVAHSRRGARIELRDVPNDEPGMTPMEIWCN"
BASE COUNT         2196 a         4605 c         4821 g         2283 t
ORIGIN      
        1 tgctcgggcg tcgcgcagaa gtcactggcc gacgcgggca gtcccatgcc ggcgacgacc
       61 gcgagcgccg gtaccatcct tgtgtaccgt tgcatcgcgg ggcctacttc ttccagaggc
      121 tgcggtcgcc gaggtaacgc gccgcgtcgc gcccgtcggt gggttggtaa tgggcgtaca
      181 gatttggcgg gaacgcctcg acgtagttcg cgtagaggtc ccggtacagc gacgggtgga
      241 tgttcgggag cttgcgccgt gcgcggaatg ccctgatcgg aatccgcgcg agcaccgggc
      301 cctcggccgg cgagttcgcc ttggcgatct gcgcgccatc gggcccgtag atcgcgctgc
      361 cacccgagct gatatcgtcg aacgagccgg ggttaccggg cgagatcgca ttgttcgcga
      421 tcgcggtgta catctggttg tagcgggcgt tggcgcggat gtcgtactcg tcgaagccgc
      481 cggtcgccgt gcgcagcatg atctcgccgc ccttcagcgc aaaggcccgg atgatctccg
      541 gctcgagctg cgtggaggtc gtgcagatat tgccgatcgg cgtgcgggtg accggcacca
      601 cttcgtcggc accgtacatc tcgacgtact ggtcgagcac gtcgaagatc gtcgtcgtga
      661 agatctcgaa cgcgccgccg aacaggccct tgatgttgcg tgccttccag tggcggccga
      721 cgatctggcc cgcgcggtcg atgacggtcg tgatcgacag gatatggttg ggccaggccg
      781 ggtcgcgggc ataggagccg aacacgacgt agcagccgta gcgcttcgcg cgcgccgcga
      841 tcgcctcggt ctcgacgccc ggaatctcga tcgcgacctt cagcagctct tcgcgccgcc
      901 agcgggcgtc gaagcccgtg atcgggaact cgtggaacat cacgaggtcc ttcggtccga
      961 gccagccgtt ggccgcgtcg atcagcccga gcatgtgctc gagattggcg gtccgggtcg
     1021 actcgagatt cctcacgtcg accgcccgca cgcgcgactg cacgacgcac agcgagattt
     1081 cgtcgcgctc gagttccttc gcctcgtact ggggatggct cattgcgggc gcccggccgg
     1141 tgctacagct gcggaccgcc cttcgccgcc gccgccgcaa gctgctcgcg cgacagctgt
     1201 gccttcatgt tcgccggcgt gcggatcgcg agccacgcgg cgatcgcgag gccgagggtg
     1261 aacagtgtca tctcgagcgc gtactcccat ggcttctccc agaactcggt gaagaggtgc
     1321 cagcggcccg cgatctccac cgcgttccac gcgatgatcg cggtcgggat gccatagcgg
     1381 accgcggtgg tcactttcca gaatttcatc aggcgcagga acaggtaacc cgcccagatg
     1441 atcatcagga agaagatcac gtaagtcaca gggtggtggt cgccgaggat gctctcgtcg
     1501 tagatgaaca gcagcatcag gtagcagaac cacagcacgt agatcgtctc gagcgaggtg
     1561 atcgcggcga aattgcgctc ctgcgcgggg ttcggcttgc cggtcttcag gtgcgcgtaa
     1621 cgctggatcc acaggaacat gttgcacttc gtctctttgt tcatgaggaa gtaggcgagc
     1681 gtcgcgccca acaggccgac cgaggacatc atcacgaggt attcgccctt ggtctggatc
     1741 tcccctgaga ccctcgggtc catcagatcc ggcacaccca gcgtctgggc cgaccagacg
     1801 aacgagaact cgatccagcc ggtccacagc agcgtgccgg cccagaagcc gtaccaggtc
     1861 gaggcgacct cattgcggtt gcgcatgccg accagcagca gtgccgcgcc ggcgaggccg
     1921 acgaggaacg ccgcgaggat cacgtggttc tcgccgaaga tgtcctccat gacgatcatc
     1981 accgtgtgtc cgagcgcctg caccacgaat acgacgatga aggccagcag gccgacgaac
     2041 ggtggccggt agagccacga cttgagcttg ctcgtcggag cggtggtgga ttgcgcgacg
     2101 ctcatgttct cgtatctcct gatgtgtggc gacctgacct tcggacacgc ggggcgtgcc
     2161 gtcaatagac acggtctgca ggccaccagt gtatggacaa gcgcgggcgc gtggtgtcaa
     2221 cggaacgtca acggcgggtc gccggggcgc aagccccaac gggccgtcgg tcgggcgtgc
     2281 ggctcagctg acctgcgccg gcggcaggtg aactgtgagc gtgagcccgc cgccgtcgcg
     2341 gggcagggca gccacgtcgc cgccatgcag cctgaacacg cggcgcgcga tcgccaggcc
     2401 gagtccgccg tcggtcgatc cgctgtcgtc ggccgatgag tcgcggtcgg cacctgctgc
     2461 gcgtggcacc ctgaaccagg gctcgaagat gcgctcaatg tattccgcgg gcactccctc
     2521 gccgcgatcg cgcacctcga tgccgatcgc atcgcccgtc acggcggcga tctcgacctc
     2581 gctgcccggc ggggcgtgac ggatcgcgtt gcgtacgaga ttgtcaaacg cgctgcgcag
     2641 cagcgcaggg tcgccgacga cgacctggcc gggcgcggcg ctcagccgca gcgccacacc
     2701 gcgcccggcg gcgatcagct cgccgtcgcg cgcgatgtcg cgcagcagtt cgtctatccg
     2761 caccaggcgg cgggccatcg aggtggcgtc gccgagccac gaataccgca ggatgtcgcc
     2821 gatcagtgcg tccatgcgcc ggatctcgcg ctcgatgcgt tcgcgatcgg ccggcgcgag
     2881 cccgccgtgg tgcgcggcga gcacggtagc ggcctgcagt cgcgtgagcg gcgagcgcaa
     2941 ttcatgtgag acgtcgcgca gcaggtgttc gcgatgctcg agcagccggg cgagccggtc
     3001 cgccatcgca ttgaagtcgg tcgcgagcgc gccgagctcg tcgcggcgcg acgcgatgcg
     3061 cgtcggtacg cgcgcctcga tgcggccgga agcgagcccg cgaacggcaa gctgcagctc
     3121 gctgatcggt cgcgtcaggg cacgcacgac cagccaggcg gcggccgcga tcaccagcag
     3181 tgccagggcg agcagcgcga gcgtcgtcct gctgctgccc cacgggccca cgttgcgcgg
     3241 catcacgaac aaggacagca cttcgccgtt tgcaccgacg agctgcgggg ccagcaccgt
     3301 ggggcgatag ttgtcaggat gcggatccgg gccgtgtgcg gcgatcgcgc gcatcatgcg
     3361 cgccgaccag ccgggcagtt cgcgtccgag cagctcggtg ccgtccggcg ccacgacata
     3421 gacagtcacg tcgccgggga tgcctgctgc gccgcctgtg agccagcggc gcagtgcgtc
     3481 gtgcccgcct cgcgtgaaca catcggccgc ctgctgcccg agcggacttt gccgcgcgtg
     3541 cacgtagcct tcgagttcgc gcgagacgag cagggcgagc gtcacgatcg cgagcgcgat
     3601 gaacagcacg atcgctgcgg cgaccgtgag gaacagccgc cagtagagcg gcgagcccgg
     3661 cgcgcggatc acggcgtgcc cgccatgacg tcgagcacgt agccgtggcc gcgcacgccg
     3721 tgcaggcgcg gattgtcgac ctgcgcgtcg gcgagcttgc gacgcagccg actcatgatc
     3781 gtatcgatgc tgcgatcgta tggttcgagc gggcgctgca tggcgaattc ggtgagctcg
     3841 gcgcgcgaga gcacttcgcc ctggcggcgc atcagcactt cgagcacgcg cagctccgcc
     3901 ggggtcaccg ccagcaaccg ctcgccggcc cacagctcga aacgcttcag gtcgagccgc
     3961 agcgtgccgg ccgagagctc atcctgcgcc gcccgcgtcg tactgccgcg acccggtgcg
     4021 cgcttcagga tcgcgcggat gcgcgcaacc agctcgagcg gattgaacgg tttgccgagg
     4081 tagtcgtcgg cgccgcgggt caggcccgcg atacgctcgg attccgcgcc gcgcgcggtc
     4141 agcatgatga ccgggacgtc ggaattctcg cgcagacgag tcagcacatc gaagccgctg
     4201 atgcccggca gcatgacgtc gagcaccacg agatcgggtg gccgggcgcg gatcgccgtc
     4261 agcgccgtct cgccacgcgc gacgccatcg acctcgaagc cgtgcagccg caggatctcg
     4321 gcgagcattg cggcgaggcc ggcgtcgtcg tcgacgagca ggatccgggg ggaggcggat
     4381 ggcggcatga tgggagcaga gaatacggca cgcggccggg tccggacagc caccggccgg
     4441 cttcgcgatt gcgcctgatt ttcccgccgc gtgacagtaa actacgcgcc gagcccgcgg
     4501 agcggctgca acggatccgc gggatgcgca actcccgcgg agtgcgcgat ttcaccccag
     4561 acccccggcg ctgcgatgat cgaaaccccc tattacctcg tcgacaaggc gaagctgctg
     4621 cgcaatctgc ggatgatcca gcaggtccgc gagcgttccg gcgcgcgcgt gctgctcgcg
     4681 ctcaagtgct tcgccacctg gtcggtgttc gacctgatgc gcgagtacat ggacggcacg
     4741 acctcgtcct cgctgtacga gctgcggctc ggccgcgaga agttcggcgg cgagacgcac
     4801 gcttactgcg tcgcctgggc cgacgccgag atcgacgagg tggtgacgta tgccgacaag
     4861 atcattttca attcggcggg ccagctcgag cgcttcgcgg cgcgcaccgc tggcctgccg
     4921 cgcggcctgc gggtcaatcc gcaggtgagc agttcgagct tcgacctcgc ggatccggcg
     4981 cggccgttca gccgcctcgg cgagagcgat ccggcgcgga tcgaggccgc gctgccgatg
     5041 atctcgggct tcatgatcca caacaactgc gagaatgccg acttcggcct cttcgaccgg
     5101 ttgctcgcgc agatcgagtc gcgtttcgca gcgctgctcg aacgcgtcga gtgggtcagc
     5161 ctcggtggcg ggatccactt caccggcgag ggttatccgc tcgaggcgtt ctgcgagcgc
     5221 ctgcgcgctt tcgccgcccg ctacggcgtg caggtctacc tcgagcccgg cgaggcggcc
     5281 gtcaccggca gtacgaccct cgaggtgagc gtcctcgata cgctctacaa cggcaagcac
     5341 ctggcgatcg tcgacagttc gatcgaggcg cacatgctcg atctgctgat ctaccgcgag
     5401 agcgcgcgga tggagccgga cgcgggcccg cacagctaca tgatctgcgg caagtcctgc
     5461 ctcgccggcg acatcttcgg cgagttccgc ttcccgcagc cactcgcggt cggcgatcgc
     5521 ctgtcgatcc tcgacgccgc cggctatacg atggtgaaga agaactggtt caacggcgtg
     5581 cgcatgccgg ccatcgcggt gcgcgagctc gacggccgcg tgaacgtgcg gcgcgaattc
     5641 acctacgagg atttcgcggc gagcctctcc tgaggcttgc ctccaaccgg tggtaccaca
     5701 ggaggttcac acacgaaatg aagagcaacg tgctgatcat cggcgccggc ggcgtcgccc
     5761 aggtggtcgc gcacaagtgc gcgcagaaca atcggctgct gggcgacatc cacatcgcct
     5821 cgcggaccgt ttcgaagtgc caggccatca tcgattcggt gcgcgcgaag cgcagcctgc
     5881 aggtggacgg cgtcctcgag gcgcatgcgg ttgacgcgat ggacatcgag gcggtgagcg
     5941 cgctgatccg cagcacgggc agccggatcg tgatcaatgt cggcgcgtcg ttcgtcaaca
     6001 tgtcggtgct gtcggcctgc atcgcgaccg gcgcggccta catcgacact gcgatccatg
     6061 aggatccgct gaagatctgc gagacgccgc cctggtacgg caactacgaa tggcgccgcc
     6121 gcgaggcctg cgagcaggcg ggtatcacgg cgatcctcgg cgccggcttc gatcccggcg
     6181 tggtcaatgc gtatgcggcg ctcgcgcgcg acgagtattt cgaccggatc gattcgatcg
     6241 acatcatcga catcaacgcc ggcagccacg ggcgctggtt cgcgaccaac ttcgacccgg
     6301 agatcaattt ccgcgagttc accggccagg tctggtcgtg gcagaacggc gagtggacct
     6361 cgaaccgcat gttcgaggtg aagaaggtct gggacctgcc cgtggtcggc gaaagcaccg
     6421 cctacctgac cggccacgac gagatccact cgctgtcgcg caatctcgat gtgccgcaca
     6481 tccgcttctg gatgggcttc ggtgatcgct acatcaccgt gttcaccgtg ctcaagaacc
     6541 tcgggctgct gtccgagcag ccggtgacga ccgctgaagg ccagcaggtc gtgccgctga
     6601 aggtggtcaa ggccgtactg cccgatccgt cgtcgctcgc gccagactac acgggcaaga
     6661 cctgcatcgg tgacctggtg cggggcgtga aggagggcaa ggagcgcgaa gtgttcatct
     6721 acaacatctc ggaccacgag gcgagcttcg ccgaggtggg cagccaggcc atctcctata
     6781 ccgcgggcgt gccggcagcc gcagcggcga tcctgatcgc ccagggcacc tgggacgtga
     6841 agaagatggt caacgtcgag gagctgccgc cacggccgtt cctcggactg ctcgggcgga
     6901 tgggcctgcc gacgcggatc cgggacgagc agggcgatcg cgccctggac ttcgccgcat
     6961 aggtcggggc gccgggcgcg tcctcgcgcg cccggcgctc agagcttgtg cgtcgagagc
     7021 agcaggctcg tctcggtgct cgagatgcct tcgatgaggc ggatccgcgc gagcacgcgg
     7081 tcgaaggcct cgaggctctc ggcgcgcagt tcggccacga tgtcccagcg gccattggtc
     7141 gaatacaggg cgctcacggc cggatcgccg cgcagcgccc tgatcaccga ctccgtgcgg
     7201 ttgccttcga cgagcactgt catcagcgcg cggatgtgct gctcctcgac ctgcggcttc
     7261 aggcgcagcg tgtagcccac gatgacgccg ctttcctcga gccgcgtgag gcgatttcgt
     7321 acggtgccgc gcgccacgcg cagcgctttc gcgagcgagg cgatcgaggc gcgcgcgtcg
     7381 acgcgcagca gtgcgatcag cttgcggtcg atgtcgtcca tggggcctcc caggtgcctc
     7441 ctgccagtat agtcaaaatg gcaagcagtc ctgacaaaat gcgcaggaat gctgtctaaa
     7501 tgggcggttt tctggctttt caatggcggc cccatcatca acactgctcc tggcgcatcc
     7561 gcggaggggt tcaatggtcg atgacaaggg tatcgggcgc atccaggcag cagggacgac
     7621 cgcggcgctg atcggcgtgc cgaccgacgt cggcgcgagc gcgcggggtg cttcgatggg
     7681 accggaggcg ctgcgcgtcg cgggcctgcg cgaagcgata gttcgccgtg gcatgcgggt
     7741 ggtcgatcgc ggcaacctgg gcgggccggc caatccctgt ctgccaccgg aacagggcta
     7801 ccggcacctg cgcgaggtcg tggagtggaa tcgacgcgtg cacgaggcgg tgtacgcgga
     7861 acttgccgcg cggcgcctgc cgatcgtgct cggcggcgat cattgcctcg gtatcggttc
     7921 gatcagcgca gtcgcacgtc attgccgcga ggcgggccgc aagctgcgcg tgctctggct
     7981 cgatgcgcat accgacttca ataccgccgc actctcgccg agcggcaatc tgcacggcat
     8041 gccgctcgcc tgcctgtgcg gcttcggccc gccggagctg atcgggatcg gcggcgcggt
     8101 gcccgcgatc cgcccggagt ggctgcgtct cgtcggcatc cgcagcgtcg acgccggcga
     8161 ggcccgcttc gtggaccagc aggcgctcga ggtcttcgac atgcgctgca tcgacgagat
     8221 cggcatgcgc gaggccatgg agcttgcgct cgccggcatc gacgcggaga cgcacctgca
     8281 cgtgagtttc gacgtcgact tcctcgaccc gcagatcgct cccggtgtcg tcaccacggt
     8341 gcagggtggg ccgtcatggc gcgaagcgca gctctgcatg gagatgatcg ccgacaccgg
     8401 ccggctcggc tcgctcgacg tcgtggagct caatccggcg ctcgacgtgc gcaaccagac
     8461 ggcggcggtc gccgtcgagt tgatcgagtc gctgttcggc aagtcgacac tgatgcggcg
     8521 ttcgccggtg gtcggcgatc attccaggcg gaatgtcacc cgcacgtgcg ttgcgtgatg
     8581 gtgccgacgt cgccgagctg cgcgttcagc cgatcgtcgc gataggccac ggccacagcc
     8641 gcggccggca aagcgatcgt gaccggcagg attcgcgccg cgaacatgtc gccaagccgg
     8701 ctccttcacg ctaaggtgcg agcctgcaat ccacacggag atcgatatga gtcattcgtt
     8761 gcgggcagtc ccggtcgccc gcgcaggtgt gttcgcggtg ctggcgatca gcgcgtgcgg
     8821 ggagcgccca gcggaggtcg aggcgcccgc cgccgaggcg cccgccgccg aggcgagcgt
     8881 cacgtccatg gaacccgaaa cggtgcgcga ggtgctctac cgctgcaaca gcggcaggga
     8941 ggtcgctgcc cgatacatgg ctggcggagg caagccgtcg cgcgtcgaac tgctgttcga
     9001 cggtgccgcc tacgagttgc acgcggtgcc cgccgcggag ggcgcgctct atgccaccga
     9061 cgatggccgc acgtccggcc gcggcctcaa ctggcacaac aagggcgccg aggccgcact
     9121 gaccgagacg atggcgggcg agccagccgg caccggtgtg cccgtcgact cctgccatga
     9181 acagccggag agccatgccg aagtcgagtg atgccgcgcc gccgtcgaat gccgcgcaga
     9241 ggcaatctcc atcctatcgt ctggccgcgc tcgaccagga tttcatcctg ggggactcga
     9301 tgcgcggcgc gcgcttcatg ctcgagtatg cgaaggccga ggagacgctg cgccgctggc
     9361 ggatccagtc caccatcgtc gtgttcggca gtgcccgtgc acaggagaac ggcgcggggc
     9421 agttggggca atggtacgcc gaggcgcgcc gcttcggccg catcgcctcc gagcgcggcg
     9481 gcgccctgac gagcaacagc gcggagcgcc agaacgtgat cgccacgggc ggcggacccg
     9541 ggatcatggc cgcggccaac cgcggtgcga gcgaggtcgg tgcgccgtcg atcggcttca
     9601 acatcacgct gccgcaggag caggagccga atccctactc gacgcccgaa ctgaccttcc
     9661 tcttccatta tttcgccatg cgcaagatgc acctggcgat gcgcgccagc gcgctggtcg
     9721 tgtttccggg cggcttcggc acgctcgacg agctgttcga aatcctgact ctgcagcaga
     9781 cgcacaaggc gccgaagatc ccgaccgtgc tggtcagccg cgactactgg acctccgtgg
     9841 tcgacttcga cacgctggtg ggacacggca tgatcgatgc cgccgacctg gcgctgtttt
     9901 cgtatgcggc cgatgccgag gaggcgtgga cgcggttggc ccggtaacat ggcgcggtgg
     9961 cccacttcct gatcgccttc ccggcgctgt tctcgatcat caaccccgtc ggtggcagct
    10021 tcgtcttcct gtcgctgacc cggcactatc caccgcaggt gcgcgcgcaa ctcgcgcgct
    10081 gggtcgcgat ctacgccttc gcgatcatca tcggctcggt gtatatcggc gcctatgtgc
    10141 tcggtttctt cggcatctcg atacccgtcc tgcgggtcgc cggcggcatc gtgatcgcaa
    10201 cggcgggctg gcgcgtgctg aacgcgccgg atgacgcccc gccgaaaggg gagagcgccg
    10261 ccggggacag cgcgtcggcg ctcgccgagc gcgagaccgt gcgccgcatg gcgttctttc
    10321 cgctcgcgat gccgctcacg acggggccgg gcacgatttc cgtggccgtg tcgctgggcg
    10381 cgacccggcc gcgggagttc gcgcagctgg tcaacttcgt ctggcaggtc gcgctcgccg
    10441 cgctgtgcat gtcggcggca atctacctgc tgttccgcta ttccgatcgc gtgttgcgcg
    10501 ccctcgggcc gaccggctcg gtgatcatcc agcgtctgtc ggcgttcctg ctgttctgca
    10561 tcggcgtgca ggtgttctgg agcggagtga gcgagctgct cgcgcagcag gcggcgttgt
    10621 agtcaggcgg cgtccgactt gcgcggcttg cgggcctcgc cgtaggcccg cgcgaaggcc
    10681 tcgacgaaca cgtgcggcag ctggtttgcc ggcatctcgg tgatgtttcg gtagaaatcg
    10741 gcgtacagat cccagtactg gccgcgctgc acgctgcccg ccttgccttt cttcagtgcc
    10801 cgctcgaaac ggccctcgag ctcggcgggg tcgaggcgat cgatgaattc gatgaacgcc
    10861 gcgcgcagtg cggcgccggc ggccttttcg tggttgcgag tgtcctcgaa gcggtcgcgc
    10921 aaccattgca cggcatcgat gcgccggctg tcatggctcg cgagcatgct cgtcaccagc
    10981 tcgtcgactg cgccccggtc gagcgggaag ggctcgtcgg ccgcggaggc gggcaggtcg
    11041 atgcggtagt tattgcgcat ctcgtgatgg tgtcggctca ggtccttgag gccgagcagg
    11101 gcctcgcgga acatctggcc ggcgaggtgg agcatgcgtg tctgcgcgtc ggccggcagg
    11161 cgatcgggat cgatgccggc gccgcggcag aaggcggcga gcccggtctg cacgtcgtac
    11221 aacgctgcgg gctgcgcctg ctcctgggcc cgcgcggccc gcgcgagccg ctccatgcgc
    11281 cgcgccacga catcggcctg gcgttcgatg ccggagtcgc gcgccgggtc gaacggcgtc
    11341 ttcacggcct ggccaaatgc gttcaccgcg cggaagctgt cgctcggcga gacatccatc
    11401 gacagcaggg atgccatgtt gatcgatgcg ccgatgtcgc cgtgtgtcgc gaccgggtcc
    11461 ggccggccgg cgcctagcac gtccagcgcg acgacggcgc tctcgtccgg ggggaagtcg
    11521 gtcgccgcat cgatcgccac cagcaactcg tattcgccga tgcgcacaat gtcgccattg
    11581 gcaagcgtgt gcgtgccgtg cttgccgatc ggcctgtcct ggtcgttgac gaaggcaccg
    11641 ttcgtcgaca ggtcctcgag caaccacttg ccattgcgaa agtgcacgcg tgcgtggcaa
    11701 ctcgagacgt agcgcatcgg atcgggcagt acccagtcgt tgtcggccgc gcggccgatg
    11761 gtgccgcccc cgacgccgaa cacgatcgtc cctcgccccc cgagggaacg tcgctgtgaa
    11821 ctgacgactc gcagccttag cgccatgcgg gatacgacac ggaatataat gggcaactgt
    11881 aagttaacaa gcggcccccc cgtcccgatg gcagccagtc acattttcaa gatcatgttc
    11941 gtgaaccagg gcaaggtcta tgaaatctac gcacgcaagg tccaccacgg gaatctcttt
    12001 ggattcatag agatagaaga acttgttttc ggcgaacgga gctccgtggt ggtcgatccg
    12061 ggcgaggagc gggtcaaggg cgagttcgag ggggtcaagc gcacctggct gccgctgcat
    12121 tcggtgctgc gcatcgacga ggtgaagaag cagggcgtca gcaagatcac cgctttcgag
    12181 ggcggtaacg tggcgcagtt tcccgggccg atctacacac cgcctccggg cagcgaggga
    12241 cctcgcaagt aggtcgctcg cacgatgctt gtactcaagg gcgctcccgc actttcaccg
    12301 tttcgactcg cgaagctgct cgggcgcctg cgttcgctcg ccccgtcggc gagcgcaatc
    12361 gatgcgcatt tcatccacat cgtcgatgtc gcccgcccgc tggaagcggc cgagcgcggt
    12421 ctgctcgact cgctgctgac ctacgggccg cgcatcgtgg cggccgcggc cggcggcccg
    12481 ggcgtgcgcc ggcttatcgt cgtaccgcgc ccgggcaccg tgtcgccgtg ggcgagcaag
    12541 gcgaccgaca tcgcgcgggt ctgcggcctc gatgccgtaa ggcgcatcga gcgcggcatc
    12601 gagtaccgca tcgccggcgt cggcgggctc gatgccgaag cgctgcggcg gctcgcggcg
    12661 gtgctcttcg atcgcatgac cgaggcggta ttcgtcgacg agacggaact cgcggcgctg
    12721 ttcgtccacc cggagccggg cgtcgtgcgt accgtgccgc gcgccggtgg ccgcgcggcg
    12781 ctcgacgatg cgaaccgcga actcgggctt gcgctgtcgg atgacgagat cgactacctg
    12841 gtcgaggcct tcgagcgcct cggccgcgat gcgaccgacg tcgaactgat gatgttcgcg
    12901 caggcgaatt ccgagcactg ccgccacaag atcttcaatg ccgactggat catcgacggc
    12961 gtgacgcagg cgaaatcgct gttcgcgatg atccgcaaca cgcacgcgcg cgcgccgcgc
    13021 ggcgtgctct ccgcgtaccg ggacaatgcc gcggtcatcg agggcagcat cggcgcgcgc
    13081 tggttcgccg accctcgcgg tggtgtctac cgcagcagcg tcgagtccat cgatatcctg
    13141 atgaaggtcg agactcacaa tcatccgact gcgatctcgc cgtttccggg cgccgcgacc
    13201 ggcgctggcg gggagattcg cgacgaaggc gcgaccggcc tcggcgcgaa gccgaaggcg
    13261 ggactcgccg gcttctcggt gtcgcacctg cgactgcccg gtctgccaca gccctgggaa
    13321 gatgcgtttg gcaagccgga ccgcatcgca tcggccctcg acatcatgct cgagggcccg
    13381 atcggcgcgg cggccttcaa caacgaattc ggccgcccga acatctgcgg ctatttccgc
    13441 aacttcgagt tgcgtggtgc gggcgatccc gcgagccgtg tgcgtggcta ccacaagccg
    13501 atcatgatcg cgggcggggt cggcaacgtg cgccgcgacc acgtcgagaa gggcgaagtg
    13561 ccggtcggcg cgaaaatcgt ggtgctcggt ggtccggcga tgctgatcgg gctcggcggc
    13621 ggcgcggcat cgtcggtcgg cagcggtgcg tcgagcgccg cgctcgattt cgcctcggtg
    13681 cagcggggca acccggaaat gcagcgccgt gcgcaggaag tgatcgaccg ctgctgggcg
    13741 atgggcgcgg cgaatccgat cctgctgatc cacgatgtcg gcgccggcgg cctctcgaac
    13801 gccgtgcccg aggtcgtcgc acacagccgg cgcggtgcgc gtatcgaact gcgggacgtg
    13861 ccgaacgacg agcccggcat gaccccgatg gaaatctggt gcaac
//
LOCUS       DAOQVY010000058        13702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_5048_length_13702_cov_5.907306, whole genome shotgun sequence.
ACCESSION   DAOQVY010000058 DAOQVY010000000
VERSION     DAOQVY010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13702
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5048_length_13702_cov_5.907306"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PKN91_06965"
     CDS             complement(<1..414)
                     /locus_tag="PKN91_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNS27848.1"
                     /translation="MIAPGDDVLARVPGAADGPFEVAALRGGLTNRSFLVGTALGRFV
                     VRLGTGADALLGIDRHTEAAAQRLAATVAVAPRVIDADESRGLLITEYVAGRAWGQSD
                     FATPGQIDRLGSQLARLHALDAHASANLPARDPLAA"
     gene            complement(411..1022)
                     /gene="pnuC"
                     /locus_tag="PKN91_06970"
     CDS             complement(411..1022)
                     /gene="pnuC"
                     /locus_tag="PKN91_06970"
                     /inference="COORDINATES: protein motif:HMM:NF016837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNS27849.1"
                     /translation="MTAFLAQLLADLRATSALEGAAVAAGLVYVVLAARRSRWCWLFG
                     GLSSAGLAWLASRSALPMQAALHSFYVGMSVYGFHRWSAERDAGGHVKVGWWPWPLHV
                     IAWLAVCAATVVSAHWLARYTHAAQPMLDAACTYGSLLATWLVARARIENWLYWIVID
                     AALGVLFATQGLALVALLYFAYLVIAAVGFASWWRKRPAAAAA"
     gene            complement(1034..1279)
                     /locus_tag="PKN91_06975"
     CDS             complement(1034..1279)
                     /locus_tag="PKN91_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27850.1"
                     /translation="MDIGVEVSLYPLDREFIPPIQDFIDRINAHAGLRITTNSLSTQI
                     FGAYDEVMAALASELRPTFEGNAKAVFVMKIIGPYPG"
     gene            complement(1292..3400)
                     /locus_tag="PKN91_06980"
     CDS             complement(1292..3400)
                     /locus_tag="PKN91_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS27851.1"
                     /translation="MIWRLIAFAHFLGVPFAFVANAAAAELDEVLVVSSRLREAALDA
                     LPASVTVLSGETVAAAGLQHFGDVLPLVPNLNWAAGSARPRYFQLRGIGELEQYQGAP
                     NPSVGFLIDDIDFSGVGMPATTFDVEQIEVLRGPQGTVYGANALAGLISVRTRDAQPQ
                     FELRGEATVGENGTWGGGGVIGGPVDGAGTAAFRVVAHHYQSDGFRRNAFLGRDDTNG
                     YDEDTLRARVKWQPTADFDLGFTAMLVDIDDGYDAFAVDNSRVTLADHPGVDQQRSAA
                     GALRLEYGGFAAATLRSVSTYADSKIAYSFDGDWGNDADWGEYAPYDYTSRFDRRRRA
                     LSEDLRLVSTGTAASPGDRAWVLGAYALDVEERNVQVDAASGEAYRALSSDYRATNLA
                     LYGEVDWRPGARTVLSIGARGERRESDYVDVDAVAGTSSAFGPSDTMWGGHVGLSFAQ
                     TERRLWYATLSRGYKAGGFNIGSAVPSDRRQFGPESLWSAEAGFGWRSADGRWSSRTA
                     LFYMRRDEQQVMSSQQLVPGDPLSYVFFTDNAARGENYGFEGSMQWAVSPRFDVAATL
                     GLLETAYLGYLTGDPARDAALDGREQAHAPQYQYSLTLAWHLPAGFFARADLQGVDDF
                     YFDTSHEQRSGAYTLANLRVGYTGERWSVSVFARNLFDERYAMRGFYFGNEPPDFANK
                     RYVQLADGRLVGLTLGFSFD"
     regulatory      complement(3388..3496)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3547..4446
                     /locus_tag="PKN91_06985"
     CDS             3547..4446
                     /locus_tag="PKN91_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HNS27852.1"
                     /translation="MSRTLDVFQVDAFTTRRFAGNPAGVVLGADALTDEDMLAIAREL
                     NNADTAFVLSPDAADHDVRIRFFTPRTEAAFVAHATVAAHYVMSRRPGSRRLRQKQKS
                     GIVEVGIRGEGDARRVAIHQAPPQIGRELLPRERLAVLDALALSSGDLDPRCPLLVCG
                     TASTRLMVGVRGAEQLRQLKPDAARLTTLSAQLGAAGYFVFTLTPQLPGVLTESRMFC
                     PAIGIAEDPVSGNAHGMLGVYLAARDLLPRSGDQAAFSGAQGHYMNRPGRVDIELDYA
                     NGIVDAVRVIGDAALVFETSITL"
     gene            4453..4797
                     /locus_tag="PKN91_06990"
     CDS             4453..4797
                     /locus_tag="PKN91_06990"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNS27853.1"
                     /translation="MDLRFAPVAALEDLRPGHMHACMIQGREVLVCHTREGVYAVDNI
                     CTHAFARLHEGRLRGTRLVCPLHGASFDVRDGRVLKGPASEALPAHAVRVTEGVIEVA
                     VDPSAPPQPSED"
     gene            complement(4794..5360)
                     /locus_tag="PKN91_06995"
     CDS             complement(4794..5360)
                     /locus_tag="PKN91_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNS27854.1"
                     /translation="MSAVEVAVIYTIGERRMESAGDAWYVAPGAQVIGSIRLGCDASV
                     WFGAVLRGDNDWIEIGDETNVQDGTIIHTDEGAPTHIGRGVTIGHRALLHSCTVGDES
                     MIANGAMVLDGARIGRHCIIAAGALVPPGREIPDGSMVMGSPGRITRQLGERDLAMIT
                     HAAAHYCARARLYREALRVDPRSGAGQP"
     gene            complement(5375..6496)
                     /locus_tag="PKN91_07000"
     CDS             complement(5375..6496)
                     /locus_tag="PKN91_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27855.1"
                     /translation="MTESTLKPFAAGDVFVAVTELNDPLDDHAGRGRIIQYDADLRPR
                     GTLWLGATTHLVGGLKFDARGVLWAFDSHAFVVLNIHPDGRLQIRDELPTRAFSHVNF
                     AKDGSFYLGEHLVGDTIRPEIAGRMKTKLPVFPGGDRFGDGHVWHFSADGKLLYEYAT
                     QTHGGMGGFLGVTNSALAPDGRTLVYCSETGPRLMRYDLVHDRQLPDLQSFQPPFQGP
                     PEMFFGMAYGPDGTLYVLRGARIDFVNATGETLRSIPLEGFGWATITVTADGRFAYVG
                     NFFTGQVAKVALGAGTIARTVNTGQARALAGIAVRAGRVRKSAKKKVAKKKVVARKKK
                     SVAGKSRKAAKRPAGPAVKKRPTRRAVAKRPVRRAARKK"
     gene            complement(6523..7728)
                     /locus_tag="PKN91_07005"
     CDS             complement(6523..7728)
                     /locus_tag="PKN91_07005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS27856.1"
                     /translation="MKPLLTLFIVCVIDVLGFGVIVPLIPYMGTRFDASPALITAILG
                     THALCQLVSAPLWGRLSDRYGRRPILMYTFAGACVSYAILGFAPNIEWILVSRILGGL
                     MAGNISAAMAYASDISSERDRAKALGTVGAAIGIGFMLGPAIGGVLAGEDVATASFLR
                     PALASMALSVLAIALVALRLPESHTAEHRALAARDAAPRRAPLQLLARIPALRYVTLA
                     ALLVAIAQAIFESIFALWALARFGYGPRTVGLLLLALSFIPVIMQGGLVRILVPRFGE
                     YRVAYAGIGAFVVGMLIAALSPGIGVAVVGLAICGAGSGAFLPSGSALASKQASGNNR
                     GSVLGTYQASSSLGRVIGPFVSGAVFARFGTGAPLVLGALIAAPAVLGIALSRRHAQA
                     QQAGRDNLP"
     gene            complement(7741..8592)
                     /locus_tag="PKN91_07010"
     CDS             complement(7741..8592)
                     /locus_tag="PKN91_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27857.1"
                     /translation="MKTQAMRRLAVALLVLVAGACARHEAPPVGVAVAGGNMPALPPL
                     APQDAKLEPAALEAAANIARYAGSDALLIARNGHLLLERYWHDTTFATPVAAGAWQGV
                     IDDLLVGALVNDRRLIDTEALPSRGAIEQAAGSDLAAYLSKRLWRPIGAADAMLAPDL
                     LAAQGDWIRIGEILANDGVYQGEEIVPPGWVQRLLARRAVPLDATGAMPDRGAYRLPG
                     AGGSCLWIVPTLRLVVLRTGGQADADRASPDATILDAVIRGAIDRPRAAHADGDEPDP
                     SVFVPTH"
     gene            complement(8589..10070)
                     /locus_tag="PKN91_07015"
     CDS             complement(8589..10070)
                     /locus_tag="PKN91_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS27858.1"
                     /translation="MPSDGPAGRSRYITVELAHIDLDRAGRAVLRDIHWRIRPGERWL
                     LFGANGAGKTQLLKLVSGAVWPKPTGRGRRHYRYAGELHRTPQDVIGEIAYLGPERQD
                     RYQRYGWDSTVERVVATGHFRSDVLLDRVDARARTAVRAALATLGIGRLAGRSFLSLS
                     YGERRLVLIARALAARPRLLLLDELFTGLDADFRARVSAWLERTARSRRPWVLAAHRR
                     ADIPRAATHALVLDRGRIVYRGGIARAPLAQWFGTGGARRRQPKRRAGRVLVRFTHAD
                     VYLDGVQVLHDLDFELRAGECWVVHGGNGSGKTTLLRTLYGDFGVASGGTVYRDGIEP
                     GVALEVFRLRVGLVAPHLQADHPQHLTVAEIVQSGARASIGLAARATARERAAADAAM
                     REFGVLRFAARPLAELSYGQARRVLFARAAVGRADLLLLDEPFSGVDPRTRADLLRRL
                     ERRAAAGAAIVMATHHRDEWPDIATHELELHRGRIVYQGLRRI"
     gene            complement(10060..10884)
                     /locus_tag="PKN91_07020"
     CDS             complement(10060..10884)
                     /locus_tag="PKN91_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS27859.1"
                     /translation="MSISPARAESIAPGVHRVLAPNASMMTVPGTNTWLLGDPPVAVL
                     DPGPALPAHVDAILAAAPRLETIFVTHTHRDHSPAVRALKAITGARVIGRGAPRDGLQ
                     DDTFVADVEPARDELFAIGGLRLRAIDTPGYASNHVCWLLEGARLMFSGDHILEGVTP
                     VILVPDGDMGAYLEALARLKGYAPRAIAPGHGRVMGEPVKVIDALVAHRLARERRVRA
                     ALVARERSTLDELLVPVYADVPADRHMLARMTLEAHLIKLVRDGHAVRDGEQWRAI"
     gene            complement(11017..13002)
                     /locus_tag="PKN91_07025"
     CDS             complement(11017..13002)
                     /locus_tag="PKN91_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNS27860.1"
                     /translation="MRYRDSRVVAKVTAAAMAVACAFVCVARAAEPPHPAEVLIERGV
                     EAIRTSPDSSLQDAQAALAELGKRPDVDLEIRARLILCDYWSERDQNMLDIEVAAIEA
                     LLPRATRRGLRAGMLVCQGEMHETLADISQALAYYEQAVRVAEEARDDEMLASALVSR
                     GYVLGLQGEYARGLADLRRAQGLYEALGMRHHAITAMNGIAILYNRMGDYAQARDIYL
                     SALEAQRAANMLREQAVTLHNLGRAHEYLQEWPEARRAFTESLAVNREIRYARGQAYA
                     LRGLAAVENGAGNWRAALETLKQAATLQAETPDARLGAQIDLARGVALHGLGRLEESA
                     AALRAAIDIFKSGDARGELATGHSELAAVEAARGDWRSGYTQLALAKQVSERLLRNQI
                     DQRFATLRVEFDTAAKEAENAILLRDNRASEQALEQSRAVRRLQAIVIALGVLLVVLL
                     ATLAIHQRRSTLRMRTLALTDELTGAPNRRAVLGRLASALTREGAGPCTILITDIDHF
                     KGINDRYGHPTGDEVLKIMAGAVRNALREPGFLGRLGGEEFLIVLPNTTLAEGRVTAE
                     RMRECVAAVDLAHICPVGRNITVSIGVTVSVNGDTPSTMLQRADEALYEAKRAGRNAV
                     RVCPPPGASVPLANGAQRDAVDQSRGDGLRDAPRPTA"
     gene            13241..>13702
                     /locus_tag="PKN91_07030"
     CDS             13241..>13702
                     /locus_tag="PKN91_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNS27861.1"
                     /translation="MEFKDYYATMGVARDATAEQIKQAYRRLARRYHPDVSKDPDAEA
                     RFKEVGEAYEVLRDPEKRAAYDQLGSGPRPGEQFRPPPDWGAGFGFRQGARPAGDDGG
                     FTFEGDPSEFFETLFGRARTAGGRAHRAREQGADHHARVIVDLAASLEGGTR"
BASE COUNT         2241 a         4886 c         4612 g         1963 t
ORIGIN      
        1 ggcagcgagc ggatcacggg cgggcagatt tgccgacgcg tgcgcgtcga gcgcatgcag
       61 gcgcgcgagt tgcgagccga gcctgtcgat ctgcccaggc gtcgcaaaat cgctctgccc
      121 ccatgcgcgg ccggccacgt attcggtgat cagcagtccg cgcgactcgt cggcatcgat
      181 cacgcgcggc gcgacggcca ccgtcgcggc aaggcgttgc gccgcagctt cggtgtgccg
      241 gtcgatgccg agcagcgcgt cggcgccggt gccgagccgt acgacaaagc ggcccagtgc
      301 agtgccgacg agaaagctgc ggttcgtcag cccgccgcgc agcgcggcga cctcgaacgg
      361 cccgtcagcc gcgccgggca cgcgcgcgag cacgtcgtcg ccgggcgcga tcatgcggcc
      421 gcagccgccg gccgcttgcg ccaccagctc gcaaagccca ccgccgcgat cacgagatag
      481 gcgaaataga gcagcgccac gagtgcaagc ccctgcgtgg cgaacagcac cccgagcgcc
      541 gcatcgatca cgatccagta cagccagttc tcgatgcgcg cgcgcgccac gagccaggtg
      601 gcgagcaggc tcccgtaggt gcaagcggca tcgagcatgg gctgtgcggc atgcgtgtag
      661 cgcgcgagcc agtgggccga cacgacggtc gccgcacaga cggcgagcca ggcgatgaca
      721 tggagcggcc agggccacca gccgaccttc acatggccac cggcatcgcg ctcggcgctc
      781 cagcggtgaa agccgtacac cgacatgccg acatagaagc tgtgcaacgc cgcctgcatc
      841 ggcaaggcgg agcgcgaggc gagccacgca agacctgcag acgagaggcc gccgaacagc
      901 cagcaccagc ggctgcgtcg cgcagcgagc accacataga cgagcccggc cgcaaccgcg
      961 gcgccctcca gcgccgacgt cgcgcgcagg tcggccagca attgcgcgag gaaggctgtc
     1021 acatgccccg ctgtcagccg gggtacggac cgatgatttt catcacgaac accgctttcg
     1081 cattgccctc gaaggtcggg cgaagctcgc tcgcgagtgc ggccatgacc tcatcgtagg
     1141 caccgaagat ctgcgtgctc aggctgttgg tggtgatcct cagtcccgcg tgcgcattga
     1201 tgcggtcgat gaagtcctgg atcggcggaa tgaactcccg gtcgagcggg tacaggctga
     1261 cttcgactcc gatgtccatc tggtgctccg atcagtcgaa agaaaagccg agggtgagcc
     1321 cgacgaggcg cccgtcggcg agctgcacgt agcgcttgtt ggcgaaatcg ggcggctcgt
     1381 tgccgaagta gaaaccgcgc atcgcgtaac gctcgtcgaa cagattgcgc gcaaacacgc
     1441 tgaccgacca gcgctcgccg gtgtagccga cgcgcaggtt cgcgagcgtg taggcgccgg
     1501 agcgctgctc gtgactcgtg tcgaagtaga agtcgtcgac gccctgcagg tccgcacggg
     1561 cgaagaagcc cgccggcagg tgccaggcga gcgtgagcga atactggtac tgcggcgcat
     1621 gcgcctgctc tcgcccatcg agcgcggcat cgcgcgccgg gtcgccggtc aggtagccca
     1681 agtaggccgt ctcgagcagt cccagcgtcg ccgcgacgtc gaagcgtgga gagaccgccc
     1741 actgcatgct gccctcgaag ccatagttct cgccgcgcgc cgcgttgtcg gtgaagaaca
     1801 catacgacag cggatcgccg ggcacgagct gctgcgagct catgacctgc tgctcgtcgc
     1861 gccgcatgta gaacagcgcc gtgcgcgacg accagcggcc gtcggccgag cgccaaccga
     1921 atcccgcctc ggcgctccac agtgattcgg ggccgaactg ccggcgatcg gacggcaccg
     1981 cactgccgat gttgaaacca cccgccttgt agccacgaga cagcgtggca taccagagtc
     2041 tccgctccgt ctgcgcaaag ctcaggccca cgtgcccgcc ccacatcgtg tcggaggggc
     2101 cgaatgcgct cgacgtcccg gcgaccgcgt cgacgtcgac gtagtcggat tcgcgccgct
     2161 cgccgcgcgc accgatcgac agcacggtgc gcgcacctgg ccgccagtcg acctccccgt
     2221 acagtgcaag gttcgtcgcg cggtagtcgc tcgacagcgc gcggtaggcc tcgccgctcg
     2281 cggcatcgac ctgcacgttg cgctcttcga catcgagcgc atacgccccc agcacccagg
     2341 cccggtcgcc cggcgaggcc gcggtcccgg tcgagaccag gcgcaggtcc tcgctcagcg
     2401 ctcggcgccg ccgatcgaag cggctggtgt aatcgtatgg cgcgtactcg ccccagtcgg
     2461 cgtcgttgcc ccagtcgccg tcgaacgaat aggcaatctt cgagtcggcg taagtcgaga
     2521 cgctgcgcag cgtggcagca gcgaatcctc cgtattcgag gcgcagcgca ccggccgccg
     2581 aacgctgctg gtcgactccc ggatgatcgg cgagcgtgac gcgcgagttg tcgaccgcga
     2641 acgcatcgta gccatcgtcg atgtcgacca gcatcgccgt gaacccgaga tcgaagtcgg
     2701 cggtcggctg ccacttgacc cgcgcgcgca gcgtgtcttc gtcgtaaccg ttggtatcgt
     2761 cgcgaccgag gaaggcgttg cggcgaaaac cgtcgctctg gtagtgatgc gccaccacgc
     2821 gaaaggcggc cgtgccggcg ccatcgaccg ggccaccgat gacgccaccg ccgccccagg
     2881 tcccgttctc gcccaccgtg gcctcgccgc gcagttcgaa ctgcggctgc gcgtcgcgcg
     2941 tgcgcacgct gatcagcccg gcgagcgcgt tggccccgta gaccgtgccc tgcgggccac
     3001 gcaatacctc gatctgctcg acgtcgaagg tcgtcgcggg catgccgacg ccggagaaat
     3061 cgatgtcgtc gatcaggaaa cccaccgaag ggttcggcgc gccctggtac tgctcgagct
     3121 cgccgatgcc gcgcagctgg aaatagcgcg gccgcgcgct gcccgccgcc cagttcagat
     3181 tcggtacgag cggcagcacg tcgccgaagt gctgcagacc cgccgccgcc acggtttcgc
     3241 ccgagagcac cgtgacgctc gccggcagcg cgtcgagcgc cgcttctcgc agccgcgagg
     3301 aaacgacgag cacttcgtcg agctccgcgg ccgcggcatt tgccacgaat gcgaatggca
     3361 cacccaggaa gtgcgcgaat gcaatcaaac gccagatcat gcctgctccc tacgccggta
     3421 ttagccggat caggttcaac gggttcgcct tcgacggcgt ctcaggcccc gcgcggggcc
     3481 accccaagga tgtggcggct atgttagaga tcgtgcgccg cattgcaagt gattagaatc
     3541 tcctccatga gccgcaccct tgatgtcttc caggttgacg ccttcacgac acggcgtttt
     3601 gccggcaatc ccgccggcgt cgtgctgggc gccgacgccc tcaccgacga ggacatgctc
     3661 gcgatcgcgc gcgaactgaa caacgccgat accgcattcg tgctttcgcc cgacgccgcg
     3721 gaccacgacg tgcgcatccg cttcttcacg ccgcgcaccg aggcggcctt cgtcgcgcac
     3781 gccaccgtgg ctgcgcacta cgtcatgtcg cgacgcccag ggtcgcgtcg cttacgccag
     3841 aagcagaagt ccggcatcgt cgaggtcggg atccgcggcg aaggcgacgc gcggcgcgtc
     3901 gcgatccacc aggcgccacc gcagatcggg cgcgaactgc tgccgcgcga gcgcctcgcg
     3961 gtgctcgacg cgctggcgct gtcgagcggc gatctcgatc cgcgctgccc gcttctcgtc
     4021 tgcggcacgg ccagcacgcg cctgatggtc ggtgtgcgcg gcgcggagca gctgcggcaa
     4081 ctgaagcccg atgcggcgcg gctcaccact ctttcggccc agcttggcgc cgcgggctat
     4141 ttcgtcttca ctctcacacc acagttgccc ggcgttctca ccgagtcacg catgttctgc
     4201 cccgccatcg gcattgccga agacccggtg agcggcaacg cccacggcat gctgggcgtt
     4261 taccttgccg cccgcgatct gctgccgcgc agcggcgacc aggccgcgtt cagcggcgca
     4321 cagggtcact acatgaaccg cccgggacga gtcgatatcg aactcgacta tgccaacggc
     4381 atcgtcgacg cggtgcgtgt catcggtgac gcggcgctcg tcttcgagac gagcattacc
     4441 ctgtaggcgc ccatggacct tcgctttgcg cccgttgcgg cgctcgagga tctgcggccc
     4501 gggcacatgc atgcctgcat gatccagggt cgagaggtgc tggtgtgcca caccagggaa
     4561 ggcgtgtacg cggtcgacaa tatctgcacg cacgcgttcg cgcgcctgca cgaaggtcgc
     4621 ctgcggggca cccggctcgt gtgtcccttg catggcgcat cattcgacgt gcgcgacggg
     4681 cgcgtgctga agggcccggc gagcgaggcg ctgccggcac atgcggtgcg cgtgaccgag
     4741 ggcgtgatcg aggtcgcggt cgacccgtcg gcgccgcccc agccctccga ggattagggc
     4801 tgcccggccc cgctgcgcgg atcgacgcgc agcgcttcgc gatagagtcg tgcgcgggcg
     4861 cagtaatgcg cggcggcatg cgtaatcatc gcgagatcgc gctcgccgag ttgccgcgtg
     4921 atgcgcccag gggaacccat caccatcgac ccgtcgggaa tctcgcgccc cggcggcacg
     4981 agagcacccg cggcgatgat gcaatggcgg ccgatgcgcg cgccgtcgag caccatcgcg
     5041 ccgttggcga tcatggactc gtcgccgacc gtgcagctgt gaagcagcgc cctgtgcccg
     5101 atcgtcacgc cgcggccgat atgcgtcggc gcaccttcgt ccgtgtggat gatggtgccg
     5161 tcctgcacat tggtctcgtc accgatctcg atccagtcgt tgtcgccgcg cagcaccgca
     5221 ccgaaccaga cgctggcgtc gcaccccaga cgtatgctgc cgatcacctg cgcgccaggc
     5281 gcgacatacc aggcgtcgcc cgcgctctcc atccgacgct cgccaatcgt gtagatcact
     5341 gcaacctcca cggcgctcat gcgccgcgcc gggactactt cttgcgcgcc gcacggcgca
     5401 cggggcgttt cgccacggca cgacgggtgg ggcgcttctt caccgcgggc cccgccggtc
     5461 gcttcgccgc cttgcgcgac ttccccgcca cagacttctt cttcctggcc acgaccttct
     5521 ttttggcgac cttcttcttc gcgctcttgc gtacacgccc cgcgcgcacc gcgatgccgg
     5581 ccagcgcccg cgcctgcccg gtgttcacgg tgcgagcaat cgtgccggcg cccagcgcaa
     5641 ccttcgcgac ctggccggtg aagaaattgc ccacataggc aaagcgcccg tccgcggtca
     5701 cggtgatcgt cgcccagccg aagccctcga gcggaatcga acggagggtc tcgcccgtcg
     5761 cgttgacgaa gtcgatgcgc gcgccgcgaa gcacgtagag cgtgccatcc ggcccatagg
     5821 ccatgccgaa gaacatctcg ggcggcccct ggaacggcgg ctggaacgat tgcagatccg
     5881 gcagctgacg atcgtgcacg aggtcgtagc gcatcaggcg cgggccggtc tccgagcaat
     5941 acacgagcgt gcgtccgtcc ggcgcgagcg cggagttggt cacgccgaga aagcctccca
     6001 tgccgccgtg cgtctgcgtc gcgtactcgt acagcagctt gccgtcggca gagaagtgcc
     6061 acacatggcc atcgccgaag cggtcgccgc ccggaaagac cggcagcttt gtcttcatgc
     6121 gcccggcgat ctcggggcgg atcgtgtcgc cgaccagatg ctcgccgaga tagaacgagc
     6181 catccttcgc gaaattgacg tgcgagaacg cgcgcgtcgg caactcgtcg cggatctgca
     6241 atcgcccgtc ggggtggatg ttcagcacca cgaaggcgtg cgaatcgaac gcccacagca
     6301 cgccacgcgc atcgaacttc aaccccccga cgagatgcgt cgtggcgccg agccacagcg
     6361 tgcccctggg tctcaggtcg gcgtcgtact ggatgatgcg gccgcggccg gcatggtcat
     6421 cgagggggtc attgagttcg gtcacggcga cgaaaacgtc gccggccgcg aagggcttca
     6481 gggtggattc ggtcatggcg gttccttgct cgctgtattt cctcaaggaa gattatcccg
     6541 gccggcttgc tgcgcttgcg catggcgccg cgacagcgca atgccaagca ccgccggcgc
     6601 cgcgatcaac gcgccgagca ccagcggcgc gccggtgccg aagcgcgcga acaccgcgcc
     6661 ggagacgaac ggtccgatca cgcgccccag gctgctgctc gcctggtagg tgccgagcac
     6721 actgccgcga ttgttgccgc tcgcctgctt cgaggcgagc gccgaacccg acggcaggaa
     6781 cgcgccggaa cccgcgccac agatggcgag cccgacgacc gcgacaccga ttcccggtga
     6841 cagcgccgcg atgagcatgc cgacgacgaa tgcgccgata cccgcgtagg ccacgcggta
     6901 ctcgccgaag cgcggcacga gtatgcgcac cagcccaccc tgcatgatca ccggaatgaa
     6961 cgacagagcg agcagcagca gcccgaccgt gcgcggcccg tagccgaaac gtgccagcgc
     7021 ccagagcgcg aaaatcgact cgaagatcgc ctgtgcaatc gcgaccagca gcgctgcaag
     7081 cgtcacatag cgcagcgcgg ggatccgcgc aagcaactgg agcggcgcac ggcgcggtgc
     7141 ggcgtcgcgc gcggcgagtg cgcggtgctc cgcggtatgg ctttcgggca ggcgcagcgc
     7201 gaccagcgcg atcgccagca cgctgagcgc catcgaagcg agcgccggac gcaggaaact
     7261 cgccgtggcg acatcctcgc cagccagcac gccgccgatc gcgggcccca gcatgaagcc
     7321 gatgccgatc gccgcgccca ccgtgccgag ggccttggcg cgatcccgct cgctgctgat
     7381 gtccgacgcg tacgccatgg ccgcggaaat gttgccggcc atgagtccgc cgagtatgcg
     7441 cgagacgagg atccattcga tgttcggtgc gaagccgagg atcgcgtaag aaacacaggc
     7501 tccggcgaac gtgtacatca ggatcggtcg ccggccgtag cgatcgctga gccggcccca
     7561 cagcggagcg gagaccagct ggcacagcgc atgcgtaccg aggatcgcgg tgatcagcgc
     7621 gggcgaggca tcgaagcgcg tccccatgta cgggatcagc ggcacgatca cgccgaaacc
     7681 gagcacgtcg atcacgcaga cgatgaatag cgtcagcagc ggcttcaagc tggactccgg
     7741 tcagtgtgtg ggcacgaaaa cggacggatc gggctcgtcc ccgtccgcgt gggccgcgcg
     7801 gggccgatcg atcgcgccgc gtatcacggc gtcgaggatt gtcgcatccg gcgaggcacg
     7861 atcggcgtcc gcctgtccgc ctgtccgcag cacgacgagt cgcagcgtcg gaacgatcca
     7921 caggcaggaa cccccggcgc ccggcagacg ataggcgcca cgatcgggca tggcgccggt
     7981 ggcgtccagc ggcacggcgc ggcgcgcgag caggcgctgc acccagccgg gcgggacgat
     8041 ctcctcgccc tggtacacgc cgtcgttcgc gagtatttca ccgatgcgga tccagtcgcc
     8101 ctgcgccgcc agcaggtcgg gcgcgagcat ggcgtcggcc gcgccgatcg gccgccacag
     8161 gcgcttcgac agataggcgg cgagatccga gcccgcggcc tgctcgatcg ccccgcggga
     8221 cggcagcgcc tcggtatcga tcagcctgcg atcgttcacc agggcaccga ccagcaggtc
     8281 gtcgatgacc ccctgccagg cgccggcggc gaccggagtc gcgaacgtcg tgtcgtgcca
     8341 gtagcgctcg agcagcaggt gaccgttgcg cgcgatcagc agcgcatcac tgccggcgta
     8401 gcgcgcaatg ttggctgcgg cctccagggc cgccggttcg agtttcgcat cctgcggcgc
     8461 gaggggcggc agtgccggca tgttgccgcc cgcgacggcc accccgaccg gcggagcctc
     8521 gtggcgcgcg cacgcgcccg ccaccagcac gagcaacgcc actgcgagtc gtcgcatcgc
     8581 ctgagtcttc atatgcgcct caacccctgg tacacgatcc tgccccgatg cagttccagt
     8641 tcgtgcgtgg cgatgtcggg ccattcgtcc cgatggtggg tcgccatcac gatcgctgcg
     8701 cccgccgcgg cgcgacgctc gagccggcgc agcaggtcgg cccgggtgcg cgggtcgacg
     8761 cccgagaagg gctcatcgag caacagcaga tccgcgcgac cgaccgccgc gcgcgcgaac
     8821 agcacccgcc gcgcctggcc gtaggacagc tcggcgagcg gtcgcgccgc gaacctcagc
     8881 accccgaact cgcgcatcgc ggcgtcggcc gccgcacgct cgcgcgccgt ggcgcgtgcc
     8941 gcgagtccga tgctggcacg cgcacccgac tgcacgatct cggcgacggt caggtgttgc
     9001 ggatgatcgg cctgcaggtg cggcgcaacg aggccgacgc gcagcctgaa aacctcgagc
     9061 gcaacgccgg gctcgatgcc gtcgcgatac acggtgccgc cgctcgccac gccgaaatcg
     9121 ccatacaacg tacgcagcag cgtggtcttg cccgagccgt tgccgccatg cacgacccag
     9181 cactcgccgg cgcgcagctc gaagtccagg tcgtgcagca cctgcacgcc atccaggtag
     9241 acatcggcat gcgtgaaccg cacgagcacg cggccggcgc gccgcttcgg ctggcggcgg
     9301 cgtgcgccgc ccgtcccgaa ccactgcgcg agcggcgcac gcgcaatgcc gccgcgatag
     9361 acgatccggc cgcggtccag taccagcgca tgcgtggccg cgcgcgggat gtcggcgcgc
     9421 cgatgcgcgg cgagcaccca cggtcggcgc gagcgcgccg tgcgctcgag ccaggcactc
     9481 acgcgcgcac ggaaatccgc atcgagcccg gtaaagagct catcgagcag cagcagccgc
     9541 gggcgggccg caagggcgcg cgcaatcagc acgagacgcc gctcgccata tgacagcgac
     9601 aggaacgagc ggcccgccag gcgcccgatg ccgagggtcg caagcgccgc gcgcaccgcc
     9661 gtgcgcgccc tggcatccac gcgatcgagc agcacgtcgc tgcgaaagtg tccggtcgcc
     9721 accacgcgct cgaccgtgga gtcccagccg tagcgctggt acctgtcctg ccgctcgggt
     9781 ccgaggtaag cgatctcgcc gatcacatcc tgcggcgtgc gatgcagctc gccggcgtaa
     9841 cgatagtggc gccgcccccg accggtgggc ttcggccaca cggcgcccga caccagcttc
     9901 agcaattgcg tcttgccggc gccattggcg ccgaacagca gccagcgctc cccgggccgg
     9961 atccgccagt ggatgtcgcg cagtaccgca cgaccggcgc gatcgaggtc gatgtgcgcc
    10021 agctcgacgg tgatgtagcg cgaccgccct gccgggccgt cagatggcac gccactgctc
    10081 cccgtcgcgc accgcgtgac cgtcgcgcac aagcttgatc aggtgggcct cgagcgtcat
    10141 gcgcgcgagc atgtggcgat ccgccggcac atcggcgtac accggcacga gcagctcgtc
    10201 gagcgtcgag cgctcgcgcg ccaccagtgc cgcgcgcacc ctgcgctcgc gggcgaggcg
    10261 gtgtgccacc agcgcgtcga tcaccttcac tggctcgccc atcacacggc cgtggccggg
    10321 cgcgatcgcg cgcggcgcgt agcccttgag acgcgcgagc gcttccaggt aggcgcccat
    10381 atcgccatcc ggcacgagga tcaccggcgt cacgccctcg agaatgtgat cgcctgagaa
    10441 catcaggcgc gcgccctcga gcagccagca cacatgattc gaggcgtacc ccggggtgtc
    10501 gatcgcacgc agacgcagcc caccgatggc gaacagctca tcgcgtgcag gctcgacatc
    10561 ggcaacgaat gtatcgtcct gcagcccgtc gcgcggcgca ccgcgaccga tcacgcgcgc
    10621 cccggttatc gccttgagcg cgcgcaccgc gggcgagtgg tcgcgatgcg tatgcgtgac
    10681 gaagatcgtc tcgagacgcg gggcggccgc cagaatcgcg tccacgtgcg ccggcagggc
    10741 cggtccggga tcgagcacag ccacgggcgg gtcgccgagc agccaggtat tggtgcccgg
    10801 taccgtcatc atcgaagcat tgggcgccag cacacggtgc acgcccggcg cgatcgattc
    10861 cgcacgagcg ggactgatgg acatccgggg aaggatagcg ggcccgcgtg cgggttgcga
    10921 taccgcgcac atgtcattcg ccggcaagtc gatcgccgtc gcgcgcggcg catgcaccgg
    10981 gccatggcgc gacgcgagcg cctgttgacc tcctcgctac gcagtcgggc ggggcgcgtc
    11041 ccgaaggccg tcgccccgcg actgatcgac cgcatcgcgc tgcgcgccat tcgcgagcgg
    11101 cactgacgcg ccgggcggcg ggcacacgcg cacggcattg cgcccggcgc gcttggcctc
    11161 atacaaggcc tcatcggccc gttgcagcat cgtgctcggc gtgtcgccgt tcaccgatac
    11221 ggtgacgccg atgctgacgg tgatgttacg ccccaccgga cagatgtgcg caagatcgac
    11281 cgcggcgaca cactcgcgca tgcgctcggc ggtcacgcgt ccttcggcca gagtcgtgtt
    11341 tggcaacacg atcaggaact cctcgccgcc gagccggccg aggaaaccgg gctcgcgcag
    11401 cgcattgcgc acggctcccg ccatgatctt cagcacttcg tcgccggtcg gatgcccgta
    11461 acggtcgttt atgcccttga agtggtcgat gtccgtgatc aggatcgtgc aggggccggc
    11521 gccctcgcgc gtcagcgccg atgcgaggcg tccgagcacc gcccggcggt tgggcgcgcc
    11581 ggtcagctcg tccgtcagcg cgagcgtgcg catgcgcagc gtcgagcgcc gctggtggat
    11641 cgccagtgtc gcgagcagca ccaccaacag cacgcccagc gcgatcacga tggcctgcag
    11701 ccggcgcacg gcgcggctct gttccagcgc ctgctcgctg gcgcgattgt cgcgcaacag
    11761 gatcgcgttc tcggcctcct ttgccgctgt gtcgaactcc acgcgcagcg tggcgaagcg
    11821 ctggtcgatc tgattgcgca gcaggcgttc gctgacctgc ttggcgagcg ccagctgcgt
    11881 gtagccgctg cgccagtcgc cgcgcgcggc ttcgaccgcg gcgagttccg aatgaccggt
    11941 cgccagctcg ccgcgcgcat cgccgctctt gaaaatgtcg atggccgcgc gcagcgccgc
    12001 ggcgctctcc tcgagccggc cgaggccgtg cagcgcgacg ccacgcgcca ggtcgatctg
    12061 tgcaccgagg cgcgcatcgg gtgtctcagc ttgcagggtc gccgcctgct tcagcgtctc
    12121 gagcgcagcg cgccagttgc cggcaccatt ctccacggcc gcgaggcccc gtagcgcgta
    12181 cgcctggcca cgcgcgtagc ggatctcccg gttgaccgcg agggactcgg tgaacgcgcg
    12241 gcgcgcctcg ggccactcct gcagatactc gtgcgcccgc ccgaggttgt gcagcgtgac
    12301 ggcctgctcc cgcagcatat tcgcggcgcg ctgcgcctcg agcgccgaca ggtagatgtc
    12361 acgcgcctgc gcatagtcgc ccatgcggtt gtagaggatc gcgataccgt tcatggcggt
    12421 gatcgcgtga tggcgcatgc cgagcgcctc gtacaggcct tgcgcgcgcc gcaggtccgc
    12481 gagcccccgt gcgtactccc cctgcaatcc gagcacataa ccacgcgata ccagtgcact
    12541 cgccagcatc tcgtcgtcgc gcgcctcctc ggcaacccgc actgcctgct cgtaataggc
    12601 gagcgcctgc gaaatatcgg cgagcgtctc gtgcatctcg ccctggcaga cgagcatgcc
    12661 tgcacgcagc ccgcggcgcg tggcccgcgg cagcaaagct tcgatcgccg ccacctcgat
    12721 atcgagcatg ttctggtcgc gctcggacca gtagtcgcac aggatcaggc gggcacggat
    12781 ctcgaggtcc acatccgggc gcttgcccag ttcggcgagc gcggcctgtg cgtcctgcag
    12841 gctggagtcg ggtgaggtgc gaatggcctc gaccccgcgc tcgatcagca cctcggcggg
    12901 atgcggcggc tcggccgcac gcgcgacgca aacgaacgcg cacgcgaccg ccatcgcagc
    12961 tgccgtgacc ttggcgacca ccctggaatc ccggtacctc aagggcgcga atgtagcgca
    13021 acttgccagc gaacgtcggc caactgcgac acacttgctt atggcaaatg cgcgccgagg
    13081 cctcggccag atttgcgccc atgcatgtcg tcatggggga ttctgtcgtg gccgcaaagc
    13141 gccaatcggc accctggccg gtgagcgccg gatcggcaag cgccgattgc gccggcgttg
    13201 cgcgatacac tggtcacatc gcaccgccgg acacggatcg atggagttca aggactacta
    13261 cgcgaccatg ggagtcgcgc gtgatgcgac ggccgagcag atcaaacagg cctatcgccg
    13321 gctcgcgcgc cgctaccacc cggacgtgag caaggatcct gatgccgagg cgcgcttcaa
    13381 ggaagtgggc gaagcctacg aagtgctgcg cgatccggag aaacgtgccg cctacgacca
    13441 gctcggcagt gggccccgcc ccggggagca gttccggccg ccgcccgact ggggagcagg
    13501 attcggattt cgccagggcg cgcggccggc gggcgacgac ggcggcttca ccttcgaggg
    13561 cgaccccagc gaattcttcg agacgctgtt cggtcgcgcc agaaccgcgg gcgggcgcgc
    13621 acaccgggcg cgcgaacagg gcgccgacca tcatgcgcgc gtgatcgtcg acctggcggc
    13681 atcgctggaa ggcggcacgc gc
//
LOCUS       DAOQVY010000059        13476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_5167_length_13476_cov_5.514716, whole genome shotgun sequence.
ACCESSION   DAOQVY010000059 DAOQVY010000000
VERSION     DAOQVY010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13476
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5167_length_13476_cov_5.514716"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /gene="tmk"
                     /locus_tag="PKN91_07035"
     CDS             <1..562
                     /gene="tmk"
                     /locus_tag="PKN91_07035"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNS27862.1"
                     /translation="HLRAQLERRGVAVRLTREPGGTPLAERIRALVLERGEEPVSAEA
                     ETLLMFAARRLHVDNLIRPALERGEWVLCDRYTDATRAYQGGGRGVAASLIESLASAV
                     HVDVNPDLTLLFDLPVALGLERAAARRGATDRIGEESVAFFERVRARYLEIARAEPDR
                     VRCIDAAQPLEAVIAAACAAVIELAP"
     gene            613..1518
                     /locus_tag="PKN91_07040"
     CDS             613..1518
                     /locus_tag="PKN91_07040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNS27863.1"
                     /translation="MNNLRGARASGRLGHALLIHADPGCGGELLADWAAQLLLCQREP
                     APCLACTDCRHCAAHQHPDVFFLQPTEDSKQIRVEDVRTFIADLVLTSHGGGYRVGIV
                     APADVLNSNAANALLKTLEEPPPRTLLALISASPSRLPATILSRCTRLRVTAPPRVKT
                     LDWLAARGRSGDLAGAAEVLGDAPLTLLDEDADAVARMSDEMLRAVAEVERGAGDPAQ
                     LAERWSRDQFALRLACLEAWLTRRIHLSANAPNGNIVWHFGLLDVLRELRREIDTPLN
                     KSVALETWLWRLAAGGQPGRVTRSQ"
     gene            1536..1901
                     /locus_tag="PKN91_07045"
     CDS             1536..1901
                     /locus_tag="PKN91_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNS27864.1"
                     /translation="MKDPASRTGTSKPGLLTLTIKDKSALYLAYMPFVNNGGLFIPTN
                     SNYRLGDEVFMLLNLMGEDEKLPVAGRVIWVTPKGAQGKRTAGIGVQFSDQDRGQTQK
                     KIETYLAGALSGDKATHTM"
     gene            complement(1912..2994)
                     /locus_tag="PKN91_07050"
     CDS             complement(1912..2994)
                     /locus_tag="PKN91_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNS27865.1"
                     /translation="MSQDQGRPATVPLLKSFAPLEGMKAENVHALAKKIQVRTLDAGR
                     VLFKEGESDKRTFWLVKGMVELRAGDRTAAMIKAGTPEARNPLTPGQPRKYTARAVDQ
                     IEYLSIDSELLDVMITWDQTGSYEVSELQADLDGGGNADDWMTTLLSTKSFHRIPPAN
                     IQAIFMRMQRQTYRAGEIVIKQGTEGDFFYAIVKGRCQVTRETPLNKEGIKLAELGVG
                     DTFGEEALIAEAKRNATVSMLTDGVLMRLNKQDFRELMNEPLLQWKTDEEAREIVAKG
                     GKWLDVRLPSEYQNLRIEDSINIPLYFIRMKLGTLDRGTKYVVVCDTGRRSSAAAFIL
                     VERGFDAYVLKGGLTSTDLGLRPPAD"
     gene            complement(3077..5122)
                     /locus_tag="PKN91_07055"
     CDS             complement(3077..5122)
                     /locus_tag="PKN91_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNS27866.1"
                     /translation="MPDAPSWTLQRDSEGIATLTLDRPGASTNVLSRPVLEALGALLD
                     QLAASPPRALVVRSGKASGFIAGADINEFVKLEGLDEAYALIRGGQQIIDRLAALPCP
                     TVAAIHGFALGGGLELALACRYRVAADDDKLSLGLPEVQLGIHPGFGGTVRTVRLLGA
                     RPALELMLTGKPVRGAKALRMGLVDRLVPRAELDAAAKALALHPPAPRRAPLGARALS
                     WPLVRPFIKPALVRQVAARARRDHYPAPYAIIDLWAKHGARGAAAFEAEARSIARLFL
                     TDTSRNLVRVFFLQDRLKALGGRTPQPLRHVHVIGAGVMGGDIAAWCALRGFDVTLQD
                     RESRFIEPALARARDYYQKRLKDEAKVAATLARLRADVAGDGVPQADVVVEAIYEKLD
                     AKRELFASLEPRMKPGALLATNTSSIKLEQLAEPLADPGRLVGLHFFNPVAQMPLVEI
                     VSSDVTHPEVANLALAFARRIDKLPLPCRSAPGFVVNRVLTPYLQEAMLALEEGVPAA
                     LIDKAAVDFGMPMGPIELADIVGLDVSEHVGMIIAQELGRTPPPLPTLERHLAAKEFG
                     RKSGRGFYEWRDGKAVKPDPGAATAPADLTDRMLLALVNECVAVLREGTVDDADLLDA
                     GVIFGTGFAPFRGGPLAYARKRGHGEIVNRLQAFAAQYGARFTPDAHWRGLDRAGKA"
     gene            complement(5088..5864)
                     /locus_tag="PKN91_07060"
     CDS             complement(5088..5864)
                     /locus_tag="PKN91_07060"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNS27867.1"
                     /translation="MRLLVYNIRYATGTGPTFHLPLPGAGYLRSNPRVLRGITQFVGA
                     EKPDVVGLIEVDTGSIRSGMVNQAAFIANELGHYTTYQSKYSASSLNQIMPVVRKQAN
                     AFLAAPHVAGERFHYFDTGIKRLIIELELDEVCVFLVHLSLKFRHRQYQMRSLHDLII
                     RSHKPVIVAGDFNTFWGTHEIYLFMRAAGLASANTLGLPSFPSRNPRAELDFILVSRE
                     IEVSDFRVPDVRFSDHRPIVCDFTVRTTRDARRPELDPAA"
     gene            5960..6412
                     /locus_tag="PKN91_07065"
     CDS             5960..6412
                     /locus_tag="PKN91_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27868.1"
                     /translation="MSQADPIRVFVSHVWEPSDDYLRVFEFLESARNFFYRNTATPEQ
                     MPAGDKEALREDLRRQIKEAEVVILLPSLYAKHQDLTTFQALFAQASSKPVVLLKFFG
                     ADTPLPKALTDLANVVVDWDERALVDAIRQEARHENTARWDVIEFKLD"
     gene            complement(6399..6755)
                     /locus_tag="PKN91_07070"
     CDS             complement(6399..6755)
                     /locus_tag="PKN91_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27869.1"
                     /translation="MSNVMDRIQRAALTLTCTGPLKERLASAYRDHLASIESADLPRD
                     LAEDLIAVRRAMLRERPLCRNEDAVQATVRKMSNEEATELAGRVVMLFGAMCGQSQQS
                     HRGATSNGAVVHINRA"
     gene            complement(6939..7742)
                     /locus_tag="PKN91_07075"
     CDS             complement(6939..7742)
                     /locus_tag="PKN91_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNS27870.1"
                     /translation="METARWLLSLIAAAWLAGCGTLPGGPAWGEEATPAPGWQRVREA
                     AWSAARNPRVWLPLAGAALLQVSDWDRDIADWARRETPLFGSNERAGEWGDHLRTASV
                     VGLHMTALATPGGDTPREWFGNKLRGYAVDSAAIVTTTELTQELKRATGRVRPNGADE
                     RSFPSGHTAVAAVHGRLAQLNLRSVDIPPGARRTLGIGLDAVTLGTSWSRIEAGWHFP
                     SDTLVSASLGAFFAQFYDDAFLHAGQTKVPTAGLQWQEGGAILTVLWIF"
     gene            7859..8338
                     /locus_tag="PKN91_07080"
     CDS             7859..8338
                     /locus_tag="PKN91_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HNS27871.1"
                     /translation="MNQGTNSAIAAAAIVAALLAGGCSRAPDASPDPDARVVVYKTES
                     CGCCGAWVTHLENAGFAVEVHDVDNLGPTKERVGVPFAMGSCHTAEVGGYFVEGHVPA
                     DDIRRLLRERPSARGLTVPAMPVGSPGMEVPSGRVDPYDVLLVAPDGSASVFAHYGD"
     gene            complement(8342..9205)
                     /locus_tag="PKN91_07085"
     CDS             complement(8342..9205)
                     /locus_tag="PKN91_07085"
                     /inference="COORDINATES: protein motif:HMM:NF017119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HNS27872.1"
                     /translation="MTAQGAAAALALLTFALVAGPQTRAQDHRHHDHGAMQSAPATNA
                     ASAARSGPVAPVPELTDADREAAFPELSGGMTHASAVNSFILLDRLEIRDADAGTGFA
                     WDAEAWFGSDINRLWLRSFGERIDGVTEGSGLEVLYGRSIAAWWDIVAGVKHDFKPGA
                     SRNWATLGVKGLMPGHFELAALAYVAEAGHFGAGIEVEYDLLITNRLILQPLVELGYF
                     GKSDPERGVVVGLQAFEAGLRLRYEFTRQFAPYIGVVHSRALGNTADQLAAEGDDMTK
                     TAVVAGFRAWF"
     gene            complement(9202..10893)
                     /locus_tag="PKN91_07090"
     CDS             complement(9202..10893)
                     /locus_tag="PKN91_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HNS27873.1"
                     /translation="MSIDSCTGVTRRRFVQGLGAGGAIVAAGGLRAASDAATAAGSPP
                     VLSGTDFRLTIVEAAVNFTGRDRLAVTVNGSLPAPILRWREGDEVVLRVTNRLPHAST
                     SIHWHGLLLPANMDGVPGLSFDGIAPGSSYEYRFRVRQSGTYWYHSHSLFQEQAGLYG
                     AIIVDPREPPRWHFDREHVVLLSDWTDMDPGALFRRLKKDSSYDNYYRRTLGDFIRDA
                     RRDGLAATLAERRGWARMRMTPTDISDVNAHTYTFLMNGTTPAANWTGLFRPGEKLLL
                     RFINGAAMTYFDVRIPGLRMTVIAADGQPIDPVTVDEFRIAVAETFDVLVEPSGQEAY
                     TIFAQDMGRTGYARGTLATRAGLADAIPPLDPRPLLTMADMGHDGMDHSGHDMPGAPK
                     HPASESGNPLVDMQTMVPAPRLDDPGIGLRDNGRSVLTYAAMRSRFDDPDGCEPAREI
                     ELHLTGHMEKFAWSFNGEKFMDAAPLRLRYGERLRIILVNDTMMTHPIHLHGLWSDLE
                     DARGGFQLRKHTIDMPPGTRRSYRVRADALGRWAYHCHLLYHMEAGMMREVRVEE"
     gene            complement(11362..11925)
                     /locus_tag="PKN91_07095"
     CDS             complement(11362..11925)
                     /locus_tag="PKN91_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27874.1"
                     /translation="MRTTRCTGVPWRHAGIAALAFGFAGSIDAQAQQLGTLRIESVPA
                     AAEVELIGGKAGVTPLSISERAIYPNDYDDARADLYGMVVLRHPGCEPLRHRVTATDI
                     RQGLQLRLDCNATVTPAAVPASTPASTAAPATTPAPPAASPEALSQRRLRQLQVLQEL
                     RDEGLISAAEEERVRRRILQPEAGPGR"
     gene            complement(11927..13414)
                     /locus_tag="PKN91_07100"
     CDS             complement(11927..13414)
                     /locus_tag="PKN91_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27875.1"
                     /translation="MLARWRPERARPTVHNSKARVPKPLAHSTALSSDRESVWIGSRL
                     RYDDEAPNQASQLMSQRNHTPGFAIAIKFAAFLLSSASLLALSTQSASAMPEFARRYD
                     ITCAACHSAFPRLNKFGENFADNNMRLPNWKETADQLGDDRLVLPAYPPLAVRAQGYV
                     QARDGKSVDPLTGATQSASTDFQSPYLVKILSSAPLSENITYYFYVILAEKGGNGEAL
                     LEDAWFRHADVFGSGIGMQLGQFQLSDLMFPREVRLPFQDFMAYRMAGITYDRGVLFD
                     RALGPIGIDLGFTNGNGIEDNLSINSPGFSRPDRMFDNDNSKTVFGRIGTEIGPVSVG
                     LFGASGKQRGATGPAGLATSARNTDKRVVGLDASGQIGGNVHWYAQYLWNSWDGFLDI
                     DPARDYRWDGGFAGVDWIANENWAFSALYNYDDAGDLAGTNTVYEGIDMNSLSLTASY
                     FFMRNVKAIFEVNVDFLSKDAPTGPYFTGHLTKENYILFGFDTAF"
BASE COUNT         2252 a         4381 c         4543 g         2300 t
ORIGIN      
        1 gcacctgcga gcgcagctcg aacgccgggg cgtggccgtg cgcctgacgc gcgagcccgg
       61 cggcacaccg ctcgccgagc gcatacgcgc cctggtgctg gagcgcggcg aggagccggt
      121 atccgccgag gccgagacac tgctgatgtt cgccgcccga cggctgcacg tcgacaacct
      181 gatccgcccg gcgctcgagc gtggcgaatg ggtactgtgc gaccgctaca ccgatgccac
      241 gcgtgcctac cagggtggcg gccgcggcgt cgcggcttcg ctgatcgaat cgcttgccag
      301 cgcggtgcat gtcgacgtga atcccgacct gaccctgctg ttcgacctgc ccgtggcgtt
      361 ggggctcgag cgcgccgcgg cgcgtcgcgg cgcgacggat cgcatcgggg aggaaagcgt
      421 cgcctttttc gagcgcgtgc gtgcgcgcta tctcgagatc gcgcgggccg agcccgaccg
      481 cgtccgttgc atcgacgccg cgcagccgct cgaggcggtc attgcggcgg cctgtgccgc
      541 agtcatcgag ctcgccccgt gagcgaaaga cccgcaatcg ccgctaccga ctgcttctgg
      601 ctcggacccg caatgaacaa cctgcgcggc gcccgcgcga gcgggcgcct ggggcacgcg
      661 ctgctgatcc acgccgaccc gggctgcggc ggcgaactgc tggccgattg ggcggcgcag
      721 ctgctgctgt gccagcgcga accggcgccg tgcctggcct gcaccgactg ccgccactgt
      781 gccgcgcacc agcatccgga cgtattcttc ctgcagccga ccgaggactc gaagcagatc
      841 cgtgtcgagg acgtgcgcac cttcattgcc gatctcgtgc tcaccagcca cggcggcggc
      901 tatcgcgtgg ggattgtcgc ccccgccgac gtgctcaatt cgaatgccgc caatgcattg
      961 ctcaagacgc tcgaggagcc cccgccgcgt acgctgcttg cgctcatcag cgcgtcgccc
     1021 tcgcgcctgc cggcgacgat cctcagccgc tgcacgcgcc tcagggtcac ggcgccgccg
     1081 cgggtgaaga cgctggattg gcttgccgct aggggccggt cgggcgatct ggcaggggcc
     1141 gcggaggtgc tgggcgatgc accgctgacg ttgctcgatg aagatgcgga cgcggtggcc
     1201 cgcatgtcgg acgaaatgct ccgcgccgtg gcggaggtcg agagaggggc gggcgacccg
     1261 gcgcagctcg cggagcgctg gtcccgtgac caattcgccc tgcggttggc ttgcctggaa
     1321 gcctggctca cccggcgaat tcacttgtca gccaacgcac caaacgggaa tatagtgtgg
     1381 cacttcgggt tgctcgacgt gttgcgcgag ctgcgacgcg aaatcgatac accgctcaac
     1441 aaatcggtgg cgctcgaaac ctggctgtgg cgcctggctg ccggagggca accgggtcgt
     1501 gtaacccgat cgcaatgaga ggcggctgac ttcgcatgaa ggacccggca tcgcgcacag
     1561 gtacgagcaa acccggtctg ctgacgctca cgatcaagga caagagcgcg ctgtacctcg
     1621 cctacatgcc gttcgtgaac aacggcggcc tgttcattcc gaccaacagc aattaccgcc
     1681 tcggcgatga ggtcttcatg ttgctgaacc tcatgggcga ggatgagaag ctgccggtcg
     1741 cgggccgcgt gatctgggtg acgccgaagg gcgcgcaggg caagcgcacc gccggtatcg
     1801 gcgtgcaatt cagcgaccag gatcgcgggc agacacagaa gaagatcgag acctacctcg
     1861 ccggcgcact gtccggcgac aaagccacgc acacgatgta gcagggccgc ctcagtcggc
     1921 gggcgggcgc agcccgaggt cggtgctcgt gaggccgccc ttcagcacat aggcatcgaa
     1981 tccgcgctcc accagtatga acgccgcagc ggagctgcgt cgcccggtgt cgcagacgac
     2041 cacgtatttc gtgccgcgat cgagcgttcc aagcttcatg cggatgaaat agagcgggat
     2101 gttgatcgaa tcctcgatgc gcaggttctg gtactcggat ggcagccgca catcgagcca
     2161 cttgccgcct ttggcgacga tctcgcgcgc ctcctcgtcg gtcttccact gcagcagcgg
     2221 ctcgttcatc agctcgcgga agtcctgctt gttgagccgc atcagcacgc cgtcggtcaa
     2281 catcgacacc gtggcattgc gtttcgcttc cgcgatcagg gcctcttcgc cgaacgtgtc
     2341 gccgacaccg agctcggcca gcttgatgcc ctccttgttg agaggggtct cgcgggtcac
     2401 ctggcagcgg cccttgacga tcgcgtagaa gaagtcgccc tcggtaccct gcttgatcac
     2461 gatctcgccg gcgcgataag tctggcgctg catgcgcatg aagatcgcct ggatattggc
     2521 cggcgggatg cggtggaagg acttggtcga cagcagcgtc gtcatccagt cgtccgcgtt
     2581 gccgccgccg tcgagatcgg cctgcagctc ggagacctcg tagctgccgg tctggtccca
     2641 ggtgatcatg acgtcgagca gttcgctgtc gatcgacagg tactcgatct ggtcgactgc
     2701 gcgtgcggtg tacttgcgcg gctgtccggg cgtcagcgga ttgcgcgctt ccggcgtgcc
     2761 ggcctttatc atggcggcgg tgcggtcgcc cgcgcgcagc tccaccatgc ccttgaccag
     2821 ccagaaagtg cgcttgtcgg actcgccttc cttgaacagc acgcgccccg cgtccaaggt
     2881 gcgcacctgg atcttcttgg cgagcgcgtg cacgttctcg gccttcatgc cctcgagtgg
     2941 cgcaaaggac ttgagcaacg gaacggtcgc cggacggcct tgatcctgtg acattacctt
     3001 tcgatcagcg ctgcattatg gtgcatcgcg gttgtgcaaa tcgtaacaca gtggtttttt
     3061 cgctccggta cgtagttcac gccttgccgg cccggtcgag cccgcgccag tgcgcatcag
     3121 gcgtaaacct cgcgccgtac tgcgcggcga aagcctggag gcggttcacg atctcgccat
     3181 ggccgcgctt gcgcgcatag gccagcgggc cgccgcgaaa cggcgcgaag cccgtgccga
     3241 agatcacacc cgcatcgagc aggtcggcat cgtcgaccgt gccctcgcgc agcacggcta
     3301 cgcactcgtt caccagcgcg agcaacatgc ggtcggtgag gtcggcaggc gcggtggcag
     3361 cgcccggatc gggcttcacg gccttgccgt cccgccattc gtagaaaccg cgcccgctct
     3421 tgcgtccgaa ttccttcgcg gccaggtgcc gctccagtgt cggcagcggc ggcggcgttc
     3481 ggccgagttc ctgggcgatg atcatgccga catgttcact gacgtcgagg ccgacgatgt
     3541 cggcgagttc aatcggtccc atgggcatgc cgaagtcgac cgccgccttg tcgatcagcg
     3601 ccgccggcac gccttcctcg agcgcgagca tcgcctcctg caggtagggg gtaagcacgc
     3661 gattcacgac gaatccgggc gcgctgcggc agggcagggg gagcttgtcg atcctgcgcg
     3721 cgaacgccag cgcgaggttc gccacttcgg gatgcgtcac gtcgctgctc acgatctcga
     3781 cgagcggcat ctgcgccacc gggttgaaga agtgcaggcc caccagccgg cccggatcgg
     3841 cgagcggctc tgcgagctgc tcgagcttga tgctcgaggt gttggtggcg agcagtgcgc
     3901 cgggcttcat gcgaggctcg agactcgcga acagttcgcg cttcgcgtcc agtttctcgt
     3961 aaatggcctc gaccacgaca tcggcctgcg gcacgccatc gccggcgaca tcggcgcgca
     4021 agcgcgcgag agtcgccgcg accttcgcct cgtccttcaa ccgcttctgg taatagtccc
     4081 gtgcgcgcgc caatgccggc tcgatgaacc gggattcgcg gtcctgcagg gtgacatcga
     4141 aaccgcgcag cgcgcaccag gcggcgatgt cgccgcccat gacgcccgcg ccgatcacgt
     4201 gcacgtggcg caagggctgt ggcgtgcggc cacccagcgc cttcagcctg tcctgcagga
     4261 aaaacacccg cacgaggttg cgcgaagtgt cggtgaggaa gagcctcgcg atcgagcgcg
     4321 cctcggcctc gaaggccgcg gcgccccgtg ccccgtgctt tgcccacagg tcgatgatcg
     4381 cgtacggcgc ggggtagtgg tcgcggcgcg cacgcgcggc cacctgcctg acgagcgcgg
     4441 gcttgatgaa gggtcgcacg agcggccacg acagcgcgcg cgcgccgagc ggtgcgcgtc
     4501 gtggcgccgg agggtgcagc gcgagcgctt tcgccgcagc gtcgagctcg gcacgcggca
     4561 ccagccggtc gacgaggccc atgcgcaagg cctttgcgcc acgcaccggc ttgccggtga
     4621 gcatcagctc gagcgccggc cgcgcgccaa ggagccgcac cgtgcgcacg gtgccgccga
     4681 agcccgggtg gatgccgagc tgcacctcgg gcaggccgag cgagagcttg tcgtcgtcgg
     4741 cggccacccg gtagcgacac gcgagcgcga gttcgagtcc accgccgagt gcaaagccgt
     4801 ggattgccgc caccgtgggg cagggcaggg ccgcgagcct gtcgatgatc tgctgtccgc
     4861 cacggatcag cgcgtaggcc tcgtccaggc cctcgagctt gacgaactcg ttgatgtcgg
     4921 cgccggcgat gaagccgctc gccttgcccg agcgcaccac gagcgctctg ggcggcgatg
     4981 ccgcaagctg gtcgagcagc gcgccgagcg cctcgagcac cgggcgcgac agcacgttgg
     5041 tcgatgcgcc cggcctgtcg agggtcaggg tggcgatgcc ctcggagtca cgctgcaggg
     5101 tccagctcgg ggcgtcgggc atctctggtg gtccttacag tgaaatcgca gacgatcggg
     5161 cgatgatcgg agaagcgcac gtccggcacg cggaaatcgc tcacctcgat ttcccggctg
     5221 acgaggatga aatcgagttc ggcgcgcggg ttgcgcgacg ggaacgacgg caggccgagc
     5281 gtgttcgcgc tggcgagtcc cgcggcgcgc atgaacaggt agatctcgtg cgtgccccag
     5341 aacgtgttga agtcgcccgc cacgatcacc ggcttgtgtg agcggatgat caggtcgtgc
     5401 agcgagcgca tctggtactg gcggtgacgg aacttcagtg acaggtgcac caggaacacg
     5461 cacacctcgt ccagttcgag ttcgatgatc aggcgtttga tgcccgtgtc gaaataatgg
     5521 aatcgctcac cggcgacatg cggggcggcc aggaaggcat tcgcctgctt gcgtacgacc
     5581 ggcatgatct gattcagcga gctcgcgctg tacttgctct ggtaggtcgt gtagtgcccg
     5641 agctcgttgg cgatgaaggc tgcctggttg accatgcccg agcgtatcga gccggtatcg
     5701 acctcgatga gccccacgac atcgggcttc tcggccccca cgaactgggt gatgccacgc
     5761 aggacccggg gattgctgcg caggtagccg gcgcccggca gcggcaggtg gaatgtgggc
     5821 ccggtcccgg tggcgtagcg gatgttgtag acgagcagtc gcaaatctgg ggtcccggca
     5881 gaggcagcaa gctacagtct atcggcgcga tgacgcacgg gcgagcgtat cgccggcggg
     5941 gtcgggtaca ctcggcccta tgtcacaggc cgaccccata cgcgtgttcg tctcccatgt
     6001 ctgggagcct tccgacgact acctgcgcgt attcgagttc ctcgagagcg cacgcaattt
     6061 cttctacagg aacaccgcca cgcccgagca aatgcccgcg ggcgacaagg aggccttgcg
     6121 cgaagacctg cggcgccaga tcaaggaagc ggaggtagtg atactgctgc cgtcgctgta
     6181 tgccaagcac caggacctga ccaccttcca ggcgctgttt gcacaggcga gcagcaagcc
     6241 ggtggtgctg ctcaagttct tcggggccga tacgccgctg cccaaggcgc tgacggacct
     6301 cgcaaacgtc gtggtcgatt gggacgagcg cgcgctggtc gatgcgatcc gccaggaggc
     6361 gcgccacgaa aacacggcac gctgggacgt gatcgagttc aagctcgatt gatatgcacg
     6421 accgcgccgt tcgatgtggc gccccggtgc gactgctgtg actgaccgca cattgcgcca
     6481 aagagcatca cgacccgacc ggcgagttcc gtcgcttctt cattcgacat tttgcgcacg
     6541 gtagcctgca ccgcatcctc gttgcggcag agcgggcgct cgcgcagcat cgcgcggcgc
     6601 acggcgatca ggtcttcggc aaggtcgcgc ggcagatccg cgctttctat cgatgcgaga
     6661 tggtcacggt aagctgacgc cagacgttct ttcagtggcc cggtgcaggt gagtgtcagg
     6721 gcggcacgct gtatccggtc catcacgttg ctcatcggta cccccgaaga cgttgcatta
     6781 cttatcaacg agctagacct gaaatatagc gcgctggatt tgaaaggcaa cgtaatccgg
     6841 cccgggcccg tggtcatacg ggggaaaagg ccacctccgg ggtctataaa tcactctaaa
     6901 tatcaaatgt aactgattta tttgccaaat atttatactc agaatatcca cagcaccgtc
     6961 aagattgcgc cgccctcctg ccattgcagt ccggcagtcg gcaccttcgt ctggccggcg
     7021 tgcagaaacg cgtcgtcgta gaactgtgca aagaatgcgc cgagggaggc gctgacgagc
     7081 gtatcggacg ggaagtgcca ccccgcttcg atgcgcgacc aggacgtacc gagggtcacg
     7141 gcatcgaggc cgatgccgag ggtacgccgc gcacccggcg ggatatcgac cgaccgcaga
     7201 ttcagctgtg cgagtcgacc gtggaccgcc gccaccgccg tatggccgga tggaaagctc
     7261 cgctcatcgg cgccgttcgg gcgtacgcga ccggtcgccc tcttcaattc ctgcgtgagc
     7321 tcggtcgtcg tgacgatcgc cgcggagtcg accgcatatc cacgcagctt gtttccgaac
     7381 cactcgcggg gcgtatcgcc accaggggtc gcgagtgccg tcatgtgtaa gccaacgacc
     7441 gacgccgtgc gcagatgatc gccccattcg ccagcccgtt cattgctgcc gaagagcggc
     7501 gtttcccggc gagcccagtc ggcgatgtcg cgatcccagt cgctcacctg cagcagcgcg
     7561 gcgcctgcaa gtggcaacca cacgcgtgga ttgcgcgcgg ccgaccacgc ggcctcccgc
     7621 acgcgttgcc agccgggggc gggcgtcgcc tcttcgcccc aggccggtcc gcccggcagc
     7681 gtgccgcacc cggcaagcca tgcagccgcg atgagcgaca gcagccatcg tgcagtctcc
     7741 atgagaccgc acgatacgcc agcctcgcag agccgcggag tcctcttgcc gtgattgatg
     7801 ccctgcatca ggccgccaca cgctgcaatc gcgcttccga tgtgggagca tgacggtcat
     7861 gaaccaaggc acgaactccg cgattgcggc ggccgcgatc gtcgcggcgc tgctcgcggg
     7921 cggctgcagc cgcgcacctg atgcctctcc cgacccggac gcacgcgtcg tggtgtacaa
     7981 gaccgagagt tgcggctgct gcggcgcctg ggtcacgcat ctcgaaaacg cgggtttcgc
     8041 ggtcgaagtg cacgacgtcg acaatctcgg gccgaccaag gagcgcgtcg gcgtgccctt
     8101 tgcgatgggt tcatgccaca ccgccgaagt gggtggctac ttcgtcgaag ggcatgtccc
     8161 cgccgacgac atcaggcggc tgttgcgcga gcgaccttcg gccagggggc ttacggtgcc
     8221 cgccatgccg gtcggctcgc cgggcatgga ggtcccttcg ggacgggtcg atccgtacga
     8281 cgtcttgctc gtggcgcccg acggcagcgc ctcggtgttc gcgcactacg gcgactagga
     8341 ctcagaacca ggctcggaat ccggcgacca ccgcggtctt ggtcatgtcg tccccctcgg
     8401 ccgcgagctg atcggcggtg ttgcccagag cgcgcgagtg caccacaccg atatacggcg
     8461 cgaactgccg cgtgaactcg tagcgcagac gcaggcccgc ctcgaaggcc tggagcccga
     8521 cgacgacgcc gcgctccgga tcgctcttgc cgaagtagcc gagctcgacg agcggctgca
     8581 ggatcagccg gttcgtgatc agcaggtcgt actcgacttc gatgcccgcg ccaaaatgcc
     8641 cggcctccgc cacgtacgcc agtgcggcca gctcgaagtg ccccggcatc aggcctttga
     8701 ctccgagtgt cgcccaattg cgtgatgcac cgggtttgaa gtcgtgcttc acgcccgcga
     8761 cgatgtccca ccaggcggca atgctgcgtc catagagcac ttcgagtccg gagccttccg
     8821 tcacgccgtc gatccgctcg ccgaacgagc gcaaccacag gcgattgatg tccgacccga
     8881 accaggcctc ggcatcccag gcaaagcccg tgcccgcatc cgcatcccgg atctcgagcc
     8941 gatcgagcag gatgaagctg tttactgcgc ttgcatgcgt catgccgccg gagagctctg
     9001 gaaaggccgc ctcgcgatcg gcgtcggtca gctcgggtac gggtgcaacg ggcccgctac
     9061 gagcggcgct cgcggcattt gtcgccggcg cggactgcat ggcgccgtgg tcgtgatggc
     9121 gatgatcctg cgcgcgggtc tgcggacctg cgaccagcgc gaacgtcagc agcgccagcg
     9181 ccgcggcggc gccttgcgcg gtcattcctc caccctcact tcccgcatca ttccggcctc
     9241 catgtgatag agcaggtggc aatggtaggc ccagcgcccg agcgcatcgg cgcgcacgcg
     9301 atagctgcgc cgcgtgcccg gcggcatgtc gatcgtgtgc ttgcgcagct ggaatccgcc
     9361 gcgcgcatcc tcgagatcgc tccacaggcc gtgcaggtgg atcgggtggg tcatcatcgt
     9421 gtcgttgaca aggatgatgc gcaggcgctc gccgtagcgc aggcgcagcg gcgcggcatc
     9481 catgaacttc tcgccgttga aggaccaggc gaacttctcc atgtgaccgg tgaggtgcaa
     9541 ctcgatctcg cgtgccggct cgcatccatc ggggtcgtcg aagcggctgc gcattgcggc
     9601 ataggtcagg acgctgcggc cgttgtcgcg caggccgatg ccggggtcgt cgagtctcgg
     9661 tgcaggcacc atcgtctgca tgtcgacgag cggattgccg ctttcgctcg caggatgctt
     9721 cggtgccccg ggcatgtcgt ggccgctgtg gtccatgcca tcgtggccca tgtcggccat
     9781 ggtgagcagc ggccgtggat cgagcggcgg gatcgcgtcc gcgaggccgg cgcgcgtcgc
     9841 cagcgtgccg cgcgcataac ccgtgcgacc catgtcctgc gcgaagatcg tgtacgcctc
     9901 ctggcccgag ggttcgacca gcacgtcgaa ggtctcggcg actgcgatcc ggaactcgtc
     9961 gaccgtgacc gggtcgatcg gctggccgtc ggccgcgatg accgtcatcc tgaggcccgg
    10021 gatgcgcacg tcgaaatagg tcatcgccgc accattgatg aagcgcagca acagcttctc
    10081 gccgggtcgg aagaggccgg tccagttcgc ggccggtgtc gtgccgttca tcaggaaggt
    10141 ataggtgtgg gcgttgacgt cggagatgtc ggtcggcgtc atgcgcatcc gcgcccagcc
    10201 gcggcgctcg gcgagcgttg ctgcgaggcc gtcgcggcgc gcgtcgcgga tgaaatcgcc
    10261 aagcgtgcgc cggtagtagt tgtcgtacga ggagtccttc ttcagccggc ggaagagggc
    10321 gccagggtcc atgtcggtcc agtccgacag cagcaccaca tgctcgcggt cgaagtgcca
    10381 gcgcggcggt tcgcgcggat cgacgatgat cgcgccatag aggcccgcct gttcctggaa
    10441 cagcgaatgg ctgtggtacc agtaggtacc tgactggcgg acgcgaaagc gatattcgta
    10501 actgctgccc ggcgcgatgc catcgaagct gagccccggc acaccgtcca tgttcgcggg
    10561 cagcaacagt ccgtgccagt ggatcgatgt cgacgcatgt ggcaggcggt tggtcacgcg
    10621 cagcacgacc tcgtcgccct cgcgccagcg caggatcggc gcgggcaggg aaccgttcac
    10681 ggtcacggcc agccggtccc ggccggtgaa attcaccgcg gcctcaacga tcgtcagccg
    10741 gaaatccgtc ccagagagga caggaggcga ccctgcggca gttgctgcgt cggacgcggc
    10801 gcgcaggccg cccgccgcca cgatggcgcc gcccgcaccc aggccttgta cgaatcgccg
    10861 gcgcgtgacg ccggtgcagg aatcgatcga catcgctcta ctccaggtcg attgaggcag
    10921 gccccggtat atgtgcgggg ctctccgcag ggcggcggct cagacgatcg gaggtcggat
    10981 cggatccggc agtgtcggcg cacacagcgc gatctgccgg ggcggcgcac tcgtgagcag
    11041 cgtgcgcaca gcacggttcg cagcccagga cagaatgagc gcaggcagat gggcggttgc
    11101 gcaatggcag ggcgcggtgc tgcagcagtc atgtccgccg tcgaggggcg tcacgggcac
    11161 tggcgcctgg ctgtcggcca ctgtatggca tggcggttca tcggcctgca tgggcgcggc
    11221 tgcgagcggc gagggggccg ccgcgaagcc attgagcacg aatgcgacac atccaaggac
    11281 cggcagcagg cgggaacgtc gaatcatcgc acgaaagcct atcgtgaaac gcggtgccat
    11341 ggcggcgaga ttgtgcctgg ctcagcgacc ggggccggcc tccggctgca ggatccgccg
    11401 ccgcacgcgc tcttcctcgg ccgcactgat caggccttca tcccgcaatt cctgcagcac
    11461 ctgcagctgc cgcagtcgac gctgcgaaag ggcttccggc gatgctgccg gcggcgcagg
    11521 agtggtcgcc ggcgctgcag tgctcgccgg cgtggacgct ggcacggcag ccggcgtcac
    11581 agtcgcattg cagtcgaggc gcagctgcag tccttgcctg atgtcggtcg cagtcacccg
    11641 atgccgcagc ggctcgcagc cggggtggcg cagcacgacc ataccgtaca ggtccgcccg
    11701 cgcgtcgtca tagtcattcg gatagatggc gcgttcactg atcgaaagcg gcgtcacgcc
    11761 ggccttgccg ccgatcagtt cgacttcggc agccgctggc accgattcga tgcgcagcgt
    11821 gccgagctgc tgtgcctgcg catcgatgct gccggcgaat ccaaaggcca gtgcggcaat
    11881 gccggcgtgc ctccacggaa cgccagtgca tcgggtggtg cgcatgtcag aacgccgtat
    11941 cgaaaccgaa caggatgtag ttctctttgg tcaggtgacc ggtgaaatac gggccggtcg
    12001 gtgcgtcctt gctcaggaag tcgacgttga cctcgaagat ggctttgaca ttgcgcatga
    12061 agaagtacga cgccgtcagg ctgaggctgt tcatgtcgat gccttcgtaa acggtgttgg
    12121 tacccgccag gtcaccggca tcgtcgtagt tgtacagcgc ggagaacgcc cagttctcgt
    12181 tggcgatcca gtcgacaccc gcgaagccgc cgtcccagcg gtaatcgcgt gccggatcga
    12241 tgtcgaggaa tccgtcccag ctgttccaca ggtactgcgc ataccagtgc acattgccgc
    12301 caatctggcc cgaggcgtcg aggccgacca cgcgcttgtc ggtgttgcgt gcactcgtcg
    12361 ccaggccggc cggcccggtc gcaccgcgct gcttgcccga tgccccgaac agcccgacgc
    12421 tgaccggccc gatttcggtg ccgatccggc cgaacaccgt cttggaattg tcgttgtcga
    12481 acatcctgtc aggtcgggaa aaacccgggc tgttgatact caggttgtcc tcgatgccgt
    12541 tgccgttggt gaacccgaga tcgatgccga tcggcccgag cgcgcggtcg aaaagcacgc
    12601 cgcggtcgta ggtgatcccg gccatgcgat aggccatgaa atcctggaag ggcaggcgca
    12661 cctcgcgcgg aaacatcagg tcggagagct ggaactggcc gagctgcatg cctatcccgc
    12721 tgccgaagac gtcggcgtgg cgaaaccacg catcctcgag cagggcctcg ccgttaccgc
    12781 ctttctcggc gaggatgacg tagaagtagt aggtgatgtt ttcgctcagg ggggcgctgg
    12841 acagtatctt gaccagatag ggtgactgga agtccgtcga tgccgattgg gtcgcgccgg
    12901 tgagcggatc gaccgacttg ccgtcgcggg cctgcacata cccctgcgcg cgtaccgcga
    12961 gcggcgggta cgcgggcagt acgagtctat cgtcgccgag ctggtcagcc gtttccttcc
    13021 agttcggcag gcgcatgttg ttgtcggcga aattctcgcc gaatttgttc aggcgcggga
    13081 aggccgaatg acaggcggcg caggtgatgt cgtagcgacg tgcgaattcg ggcattgccg
    13141 acgcactttg tgtcgaaagc gccagcaacg atgcgctgga gagcaggaat gccgcgaact
    13201 tgatcgcgat cgcgaacccg ggggtgtgat tgcgctggga catgagttga ctcgcctgat
    13261 taggtgcttc gtcatcgtaa cgtaacctgg aaccaatcca aaccgactcg cggtcgctgg
    13321 acaatgcagt tgagtgagca agcggtttgg ggacgcgggc tttggaattg tgaacggtgg
    13381 gtcgcgctcg ctcagggcgc cagcgcgcca gcaagtatta gttcgcataa tgtatattat
    13441 gttaaataat atctgactca gctgaaggct tcgagc
//
LOCUS       DAOQVY010000060        13328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_5234_length_13328_cov_6.870414, whole genome shotgun sequence.
ACCESSION   DAOQVY010000060 DAOQVY010000000
VERSION     DAOQVY010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13328
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5234_length_13328_cov_6.870414"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PKN91_07105"
     CDS             complement(<1..267)
                     /locus_tag="PKN91_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27876.1"
                     /translation="MKTITLIAAALVAGSAFAGDETMASKSFESLDKNADGRITASES
                     VVDKKLAADFATVDANSDGAVSKEEFTKWQAAAKPQAEPVEQPKQ"
     gene            527..1891
                     /gene="glmU"
                     /locus_tag="PKN91_07110"
     CDS             527..1891
                     /gene="glmU"
                     /locus_tag="PKN91_07110"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNS27877.1"
                     /translation="MPLTVVILAAGQGKRMQSDLPKVLQPLAGRPLLSHVIATATALG
                     PEAVHVVYGHGGELVRKALADSPVNWVLQAEQRGTGHAVAQAMPAIDDGSLVMVLYGD
                     VPLVRVGTLERLLAAAGPKSMSLLTAEIDDPTGYGRIVRNARGEVQRIVEEKDASARV
                     RRIREGNTGVMVAPAKLLRRWLGALRADNAQGELYLTDVVAMAVKEKIKVVPQVVGEA
                     SEVLGVNDKLQLAEVEAVLRARRARELLVAGVTLVDPARIDVRGDLETGRDVQIDVNA
                     VFEGRVRLGDRVRIGPNCVIRDSDIGADTEVFANCVIDRATIGPACHIGPFARLRPAS
                     QLARGVHIGNFVEVKNSTLGEGSKSNHLTYLGDAVIGARVNVGAGTVTCNYDGANKSK
                     THIEDGAFIGSGAMLVAPVRIGAGATIGAGSTITRDTPPDKLTVGRAKQVTIDGWKKP
                     VKKT"
     gene            2026..2718
                     /gene="modB"
                     /locus_tag="PKN91_07115"
     CDS             2026..2718
                     /gene="modB"
                     /locus_tag="PKN91_07115"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF006939.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNS27878.1"
                     /translation="MTLTPEELDALRLSFAVATRSVAMSLPLATLIAWLLVRGRFRGR
                     FALDALVHLPLVLPPVVVGYLLLVLFGRRGPIGGWLYDTFGIQLAFTTAGAALATAVM
                     SFPLMVRAIRLSLEAVDRGYEEAARTLGAGPVDRFLTVSLPLMLPGILAGCVTAFAAS
                     LGEFGAVITFVSNIPGETRTLPLALYTAIQTPDGDAAAARIATLSLMLGFAGLALAEW
                     LARRLRRTLGQA"
     gene            2715..3815
                     /gene="modC"
                     /locus_tag="PKN91_07120"
     CDS             2715..3815
                     /gene="modC"
                     /locus_tag="PKN91_07120"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HNS27879.1"
                     /translation="MIRLEAGLRRGAFRLDVAFEAPEAGVVALFGRSGCGKTTLVNII
                     GGLLTPDTGRIDIGGEVLLDTATGRNVAAENRGVGYVFQDARLFPHLTVADNLDYGLR
                     RTRGRTRGIERDEVIALLALGDLLTRRPHTLSGGERQRVALGRALLAQPRLLLLDEPL
                     ASLDAARREEVLPYLEALRARLSLPMIYVSHAFDEVLRLATHLVVLDAGRVVAQGPLA
                     DVSLDPALTAIVGPAAAGAVLDGRVVDVDAARGLARVAIGDHGELQVGAAGTAPGMAL
                     RVQVLARDVILSDRPPEGLSVRNALRGRVTTISAQDDDTRRVLVDLGCASIVAYVTRD
                     AVDALGLATGSIVWALVKSVSLRSHPFPGRDA"
     gene            3846..4628
                     /gene="modA"
                     /locus_tag="PKN91_07125"
     CDS             3846..4628
                     /gene="modA"
                     /locus_tag="PKN91_07125"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNS27880.1"
                     /translation="MFAIARRGLLTLAALTLAASHAADTPRELTVYAAASMTNALQDI
                     ADRYRQERGVAITLSLGASSALARQIEAGARADVFVSADQEWMDYLAARQLIDRGSRR
                     DIAGNRLVLIAPADSQVALDIAPGFALAAALGNARLAVADPASVPAGRYAQAALTSLG
                     VWDSVADRLANADNVRAALAFVARGETPLGIVYTTDALIEKKVRLVGSFPESTHPPIT
                     YPAALPIGARPGARAFLDYLGEAESRATLAKYGFTAVAQTAR"
     gene            complement(4600..5967)
                     /locus_tag="PKN91_07130"
     CDS             complement(4600..5967)
                     /locus_tag="PKN91_07130"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNS27881.1"
                     /translation="MKTIIEPFRMKVVEPIRLTTRADRKELLAGVHYNLFGLHSDDVL
                     VDLLTDSGTGAMSAAQWSALMRGDESYAGSPSFYRFQSAVQDLMPFRHIIPTHQGRAA
                     EAILFSILGGAGRVIPSNTHFDTTRGNIEASGAEALDLPCPEAADFASTSPFKGNMDT
                     VALAQLLGERAADVPCVMLTLTNNAGGGQPVSLANVRAVHELARRHGKPLFIDGCRFA
                     ENAWFVKQREVGQQDRGVREIVRETFSLADGMTMSAKKDGLANIGGWLALNDDELALA
                     ARNRLILTEGFPTYGGLAGRDLEAIAAGLAEVTDEEYLRYRVRCNEYIGERLLSLGVP
                     IVQPVGGHAVFVDARGFLPHIAPLEYPGQALAVALYEVGGVRACEIGSVMFGRRPDGS
                     ERPARYELVRLAIPRRVYTQSHADYLVEVFEEIARDSRSLRGLRIVQEPPMMRHFTAR
                     FAPLP"
     gene            6058..7893
                     /gene="glmS"
                     /locus_tag="PKN91_07135"
     CDS             6058..7893
                     /gene="glmS"
                     /locus_tag="PKN91_07135"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNS27882.1"
                     /translation="MCGIVGAVAERNIVPILLEGLRRLEYRGYDSAGLAVLDAGQHLA
                     RVRTVGKVKALEQAIDQQPVAGRVGIAHTRWATHGVPSERNAHPHLSRDGLAIVHNGI
                     IENHEELRADLQRLGYEFTSETDTEVIAHRIHHHLASLGDLFKAVRATVAELEGAYAL
                     AVVSAAAPDRVILAREGCPVVIGIGVGENFVASDVAALLPVTRRFVFMEEGDVAEVGL
                     TSVRIIDREGNLVERAVHESELSADAAEKGPYRHFMLKEIHEQPRALANTLQERVANG
                     RLLDAAFGPAASEIFRRTEHVHIVACGTSYHAGAVARYFIEQICKLPCSVEIASEYRY
                     RNPVVLRNSLFVTISQSGETADTLAALRLAKQAGYLASLAICNQPESSLVRESDLVML
                     TRAGPEIGVASTKAFTTQLMALSMLVVALSKYHAGDAERERGLIARLIEIPGLIEKTL
                     TLDPVIHQLAERFADKNHALFLGRGALHPIAMEGALKLKEISYIHAEAYPAGELKHGP
                     LALVDADMPVITVAPNNDLLEKLKSNLMEVRARGGELYVFADPEAGFEPSDGVTVIQM
                     PKHVSYFQAPAVYTIPLQLLAYHVAVLKGTDVDQPRNLAKSVTVE"
     gene            7940..8161
                     /locus_tag="PKN91_07140"
     CDS             7940..8161
                     /locus_tag="PKN91_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNS27883.1"
                     /translation="MASEEKPEVADPTKAAAENPYTQHYVVQKGDTLSKIAREYYGDA
                     MLYPKIFEANRDILSDPNRIKPGQRLRIP"
     gene            8181..8786
                     /locus_tag="PKN91_07145"
     CDS             8181..8786
                     /locus_tag="PKN91_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27884.1"
                     /translation="MLKRTTWITAMAVLVLAAACANQKGPATKAVADAETALAAFRDD
                     AARLLPDDLSAVDSTLASLKDSLAKGDFKAVVAAAPDLSSRITALRDATEAKKAEWEA
                     ANAAAREQWSVYAADLPKMVEAIQSRVDILGKARRLPRNVSQEAFDSAKSGLDWMTTT
                     WAEANDAFNAGNAVDAAAKAQAVKDKGNEVLGLLGLAPAAS"
     gene            8783..8956
                     /locus_tag="PKN91_07150"
                     /pseudo
     CDS             8783..8956
                     /locus_tag="PKN91_07150"
                     /inference="COORDINATES: protein motif:HMM:NF017742.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
     gene            8996..9103
                     /locus_tag="PKN91_07155"
                     /pseudo
     CDS             8996..9103
                     /locus_tag="PKN91_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9107..9502
                     /locus_tag="PKN91_07160"
     CDS             9107..9502
                     /locus_tag="PKN91_07160"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNS27885.1"
                     /translation="MASPTQRVLEEALSLGAQGLRIAQTRLEILSVDLQREKQAIARQ
                     LALAVTCGTSAALAAFACILWVALAFEPQTRFIVLGVLTGVLALVAVACGLLFWRGLQ
                     RRDHLFGQLIEALKRDSDALEPRRTRETA"
     gene            9471..9716
                     /locus_tag="PKN91_07165"
     CDS             9471..9716
                     /locus_tag="PKN91_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27886.1"
                     /translation="MNLEERAKLLEVEAELQRRTLRASLDTLQAAKGITWALAGASLA
                     RRLAIVHKAKVAGYALIGTRLLFRARRALRDRKLLPR"
     gene            complement(9670..10995)
                     /gene="rimO"
                     /locus_tag="PKN91_07170"
     CDS             complement(9670..10995)
                     /gene="rimO"
                     /locus_tag="PKN91_07170"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNS27887.1"
                     /translation="MPPATRTPRVGFVSLGCPKALVDSERILTHLRAEGYSIAPSYDD
                     ADVVVVNTCGFIDAAIDESLDAIGEAIEQNGRVIVTGCLGTRPERILERHPRVLKVTG
                     AHAYEDVIGAVHEHLPPQHDPFLDLVPPQGIKLTPRHYAYMKIAEGCNHKCSFCIIPS
                     MRGRLASRPIDEVLREGEQLVRAGVKELLVISQDTSAYGADLRYAPREWRGQAYETRF
                     LDLARGLGELGAWVRLHYVYPYRHVDAVIPLMAEGKVLPYLDVPFQHGSPTVLKRMKR
                     PAHAEDTLARIHAWRRICPELVIRSTFIAGFPGETEAEFAELLDWLDEAQLDRVGCFT
                     YSPVDGAAANALPGQVPEEIKLERQARFMERAQAISASRLARRIGARMRVLVDRIDRE
                     IAIARGPGDAPEIDGVVRIRRGGKLKVGEFADVEITGAAAYDLEARVAP"
     gene            11155..12330
                     /locus_tag="PKN91_07175"
     CDS             11155..12330
                     /locus_tag="PKN91_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNS27888.1"
                     /translation="MRDLHIAVVGAGIGGLAAALGLQRAGFRVSVHEQAPELGEVGAG
                     LSLGPTAAHALRWLGLGPALDLHAYLPENQAVRHYQDGHALQWINRGRSLLQKYGERY
                     YLIHRADVHDALAAAVRANDPDAIRLGRRCTGLEQTANSATVRFEDGTHVTADVVIGA
                     DGSRSIVRRLLFGDSGPTYTGYIAWRGLVPMERVPQSVLDPPSGIYIGPGHLVNRYPV
                     RRWQLLNFIAFAERAEWSEEGWSIPSTVDELMAEFEDWHPDVRTFLAAVPPELLFKWG
                     LFDREPLTTWHSGRTTLLGDAAHPVLPFLGHGAVLAIEDAVLLARAFAGSAEIGEALQ
                     RYEAARIPRAAFVVRESRQAVKVYHSHEPEKYAQKTGGQAADERLGLFDYNPSTISV"
     gene            complement(12303..12440)
                     /locus_tag="PKN91_07180"
     CDS             complement(12303..12440)
                     /locus_tag="PKN91_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27889.1"
                     /translation="MVSTERRAVPWLWFLVPIFSLLAFFALRSCKEAPSPPQTEIVEG
                     L"
     gene            complement(12445..13011)
                     /locus_tag="PKN91_07185"
     CDS             complement(12445..13011)
                     /locus_tag="PKN91_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNS27890.1"
                     /translation="MIRVSTLCVLLGASLGFTTATVAGPAAFTLTSGNFAPGGTLADE
                     QVFNSFGCSGGNVSPALAWSGVPEGTKSFALMVHDPDAPTGSGWWHWVVYDIPADVRS
                     LSAGAGASDGRQLPPGAVHGRTDFGIPGYGGACPPEGDPPHRYNFRLFALKVEKLGVP
                     ADATAALIGFMVNANSLGVAEIHATYGR"
     gene            13055..>13328
                     /locus_tag="PKN91_07190"
     CDS             13055..>13328
                     /locus_tag="PKN91_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27891.1"
                     /translation="MTGRFDRAYYERYYNDPRSRVTSRAEMSARGRLIGAFALYLDLP
                     VRRILDAGCGVGLLQAPLRRMLPKATYTGIEVSDYLCRRHGWVQASL"
BASE COUNT         2051 a         4522 c         4656 g         2099 t
ORIGIN      
        1 ctgcttcggc tgctcgaccg gctcggcctg cggcttggcg gccgcctgcc acttcgtgaa
       61 ctcctccttg gagaccgcgc cgtcgctgtt ggcatcgacg gtggcgaagt ctgcggcgag
      121 tttcttgtcg acgaccgatt cgctcgcggt gatgcggccg tccgcattct tgtcgagcga
      181 ttcgaacgac ttcgaggcca tggtctcgtc gcctgcgaag gcgctgccgg caaccagtgc
      241 ggcggcaatc agggtgatgg tcttcatctt gcactccttg ctgggggtgg tggcgaggaa
      301 acctaccgcg cacggcgatg cgacgtaatg tcccgaggcg gcgacacaca cgctgcgccg
      361 cggcgccggc atggctgtct gacacgcaag cgcgcggaat tcacggtgtt tcggcctgtc
      421 tcgccccgcc gacgtcggcg atgctccgac cgcgggctcc ggcctcgtcc gacggttcgc
      481 gcggcgcacg tcctacacgg acactgcgct cggatacaat cgtccgatgc cactcacagt
      541 cgtgatcctc gccgccggcc agggaaagcg gatgcagtcc gacctgccga aagtcctgca
      601 gccgctcgcg ggtcgcccgc tgctctcgca tgtcatcgcc accgcgacgg cgctcgggcc
      661 cgaggcggtc cacgtcgtgt acggccacgg gggcgagctg gtccgcaagg cgctggcaga
      721 ttcgccggtc aactgggtac tgcaggccga gcagcgcggt accggacacg cggtcgcgca
      781 ggcgatgccg gccatcgacg acggcagtct cgtgatggtg ctgtacggcg acgtgccgct
      841 ggtccgtgtg ggcacgctcg agcgactgct cgcggccgcc ggcccgaagt cgatgtcgct
      901 gctcaccgcc gagatcgacg atccgaccgg ctacggacgc atcgtgcgca acgcgcgcgg
      961 cgaggtgcag cgcatcgtcg aggagaagga cgcctcggcg cgcgtgcgcc gcattcgcga
     1021 gggcaatacc ggggtgatgg tcgcgcccgc aaagctgctg cgccgctggc tcggcgcgct
     1081 ccgcgccgac aatgcccagg gcgagttgta tctcaccgac gtcgtcgcga tggcggtgaa
     1141 ggagaagatc aaggtcgtgc cgcaggtcgt cggcgaggcg agtgaagtgc tcggcgtcaa
     1201 cgacaagctg cagctcgcgg aagtggaggc cgtgctgcgc gcccgccgcg cgcgtgagct
     1261 gctggtcgcg ggcgtcacat tggtcgatcc cgcccgcatc gacgtgcgcg gcgatctcga
     1321 gacgggccgc gacgtgcaga tcgacgtcaa tgcggtgttc gaggggcgcg tgcggctagg
     1381 cgaccgcgta cgcatcggtc cgaactgcgt gatccgcgac agcgacatcg gcgcggatac
     1441 cgaagtgttc gcgaactgcg tgatcgatcg cgcgacgatc ggcccggcct gccacatcgg
     1501 tcccttcgcg cgcctgcgtc ccgcatcgca gctggcgcgc ggcgtgcaca tcggcaattt
     1561 cgtcgaagtg aagaacagca cgctgggcga aggcagcaag tcgaaccacc tcacctacct
     1621 tggcgacgcg gtgatcggcg cgcgggtcaa tgtcggtgcc ggtaccgtca cctgcaacta
     1681 cgatggcgcc aacaagtcga agacccacat cgaggacggc gccttcatcg gctcgggcgc
     1741 gatgctggtg gcgccggtgc gcatcggcgc cggcgcgacg atcggcgccg gctcgacgat
     1801 cacccgggat acgccgcccg acaagctgac cgtggggcgc gcaaagcagg tgacgattga
     1861 tggctggaag aagcccgtca agaaaacctg aagaggggcc ggggatgttc ctgcggctcg
     1921 acctggcgcc cggcaaccgc aggctgctgc ggaacggatc gcgatgatgt tgccgcgacg
     1981 gtgcgactgc cgcgccttcg tccgtcgcct gcaccgcggt actccatgac cctgacaccc
     2041 gaagaactcg atgcgctgcg cctgagtttc gccgtggcga cccgcagcgt ggcgatgagc
     2101 ctgccgctcg ccacgctgat cgcatggctg ctggtgcgtg gccgcttccg cgggcgcttt
     2161 gcgctcgatg cgctggtaca tctgccgctc gtgctgccgc cggtcgtcgt cggctatctg
     2221 ttgctggtgc tgttcggccg tcgcggaccg atcggcggct ggctttacga taccttcggc
     2281 atccagctcg ccttcaccac ggcgggtgcg gcgctcgcca ccgcggtgat gtcgtttccg
     2341 ctgatggtgc gcgcgattcg cctgtcgctc gaggcagtcg atcgcggcta cgaagaagcg
     2401 gcgcgcaccc tcggcgccgg tcccgtcgac cggttcctga ccgtctcgct gccgctgatg
     2461 ctgcccggca tcctcgccgg ctgcgtcacc gcgttcgcgg cgagtctcgg cgagttcggc
     2521 gcggtcatca ccttcgtgtc gaatattccc ggcgagacgc gcacgctgcc gctcgcgctg
     2581 tatacggcga tccagacgcc ggacggcgac gccgccgcgg cgcgcatcgc gaccctgtcg
     2641 ctgatgctcg gcttcgccgg gctcgcgctc gccgagtggc tcgcgcggcg cctgcgccgc
     2701 acgctggggc aggcgtgatc cgcctcgagg ccggtctgcg gcgcggtgcc ttcaggctcg
     2761 atgtggcctt cgaggcaccc gaggccggag tcgtcgcgct gttcggtcgt tccggctgcg
     2821 gcaagaccac cctggtgaac atcatcggtg ggctgctgac cccggacacg ggacgcatcg
     2881 acatcggcgg cgaggtactg ctcgacacgg cgacgggtcg caacgtggct gccgagaatc
     2941 gcggcgtcgg ctacgtgttc caggacgcgc ggctgttccc gcatctgacc gtggccgaca
     3001 acctcgacta tggcctgcgc cgtacgcgcg ggcgcacgcg gggcatcgaa cgcgacgagg
     3061 tgatcgcgct gctcgcgctc ggcgatctgc tcacccggcg cccgcatacg ctctcgggtg
     3121 gtgagcgcca gcgcgtcgcg ctcgggcgcg cgctgctcgc gcagccgcgc ctgctgctgc
     3181 tcgatgaacc gctcgcctcc ctcgacgctg cgcgccgcga ggaggtattg ccctatctcg
     3241 aggcgctgcg cgcgcggctc tcgctgccga tgatctacgt gagccatgcg ttcgacgaag
     3301 tgctgcgcct cgcgacccac ctcgtggtgc tcgacgccgg gcgcgtggtg gcgcaaggtc
     3361 cgctcgccga cgtcagcctc gatccggcgc tcaccgcgat cgtcggtccc gccgccgccg
     3421 gcgccgtgct cgatggccgc gtcgtcgacg tcgatgccgc gcgcggcctc gcgcgcgtcg
     3481 cgatcggcga ccacggcgag ttgcaggtgg gcgcggcggg caccgcgccg ggcatggcgc
     3541 tgcgcgtgca ggtgctcgcg cgcgacgtga tcctctccga tcgcccaccc gagggcctga
     3601 gcgtgcgcaa cgcattgcgc gggcgcgtca ccacgatcag cgcacaggac gacgatacgc
     3661 gcagggtgct ggtcgacctg ggttgtgcca gcatcgtggc ctacgtgacc cgcgacgccg
     3721 tcgatgcatt gggcctcgcg accggcagca tcgtatgggc gctggtcaag tcggtttcgc
     3781 tgcgcagtca tccgttcccc ggccgggatg cgtagtatcc ggtcggaaac agccccggga
     3841 ccatcatgtt cgccatcgcc cgccgcggcc tgctgacgct cgccgcgctc acgctcgcgg
     3901 cctcgcacgc cgcggatacg ccccgggaac tcacggtcta tgccgcggcg tcgatgacca
     3961 acgcgctgca ggacatcgcg gaccggtacc ggcaggaaag aggtgtcgcg atcacgctgt
     4021 cgctcggcgc gagctcggcc cttgcgcgtc agatcgaggc cggcgcacgc gccgacgtgt
     4081 tcgtgtcggc cgaccaggaa tggatggact acctcgccgc ccgccagctg atcgatcggg
     4141 ggagccgtcg cgacatcgcg ggcaaccggc tcgtgctgat cgcgcctgca gacagccagg
     4201 tggccctcga tatcgcgccg ggctttgcgc tcgcggcggc gctgggcaac gcccggctcg
     4261 cggttgccga tccggcatcg gtgcccgccg ggcgctatgc gcaggcggcg ctgacgagtc
     4321 tcggcgtatg ggacagcgtc gcggaccgcc tcgccaacgc cgataacgtg cgcgcggcgc
     4381 tcgcattcgt cgcgcgcggc gaaacgccgc tcggtatcgt ctacacgacc gatgcgctga
     4441 tcgaaaaaaa ggtgcgcctg gtcggcagct tccccgagtc gacgcatccg ccgatcactt
     4501 accccgcggc cctgccgatc ggtgcgcggc ccggcgcgcg cgccttcctc gattacctcg
     4561 gcgaggcgga gtcgcgcgcc acgctggcga aatacggttt cacggcagtg gcgcaaaccg
     4621 cgcggtgaag tgccgcatca tcggcggctc ctgcacgatg cgcaggccgc gcagcgagcg
     4681 gctgtcccgg gcgatctcct cgaagacctc gaccaggtag tcggcatggc tctgggtgta
     4741 gacgcgccgc ggaatcgcga gccgcacgag ttcatagcgc gcgggccgct cgctgccgtc
     4801 gggccggcgc ccgaacatca cgctgccgat ctcgcacgca cgcacgccgc cgacttcata
     4861 gagtgcgacc gcgagcgcct ggcccggata ttcgagcggc gcgatgtgcg gcaggaagcc
     4921 gcgtgcatcg acgaacaccg catgcccgcc gaccggttgc acgatcggca cgccgagcga
     4981 cagcagtcgc tcgccgatgt actcattgca gcgcacgcgg taacgcaggt attcctcgtc
     5041 ggtgacttcg gcgaggccgg cggcgatcgc ctcgagatcg cgccccgcga gcccgccgta
     5101 ggtcgggaaa ccctcggtga ggatcaggcg gttgcgcgcg gcgagcgcga gctcatcgtc
     5161 gttcagcgcg agccagccgc cgatgttcgc aaggccgtcc ttcttggcgc tcatcgtcat
     5221 gccgtcggcg agcgagaacg tctcgcgcac gatctcgcgc acgccgcgat cctgctggcc
     5281 gacttcgcgc tgcttgacga accaggcatt ctcggcgaag cgacaaccgt cgatgaacag
     5341 tggcttgcca tgcctgcgtg cgagttcgtg caccgcccgc acgttcgcga gcgacacggg
     5401 ctggccgccg ccggcattat tggtgagggt gagcatcacg cagggtacgt ccgcggcgcg
     5461 ctccccgagc agctgcgcga gcgccacggt gtccatattg cccttgaagg gcgaggtgct
     5521 cgcgaagtcg gccgcctcgg ggcacggcag gtcgagcgcc tcggcgccgc tcgcctcgat
     5581 atttccgcgg gtcgtgtcga agtgcgtgtt ggagggaatc acgcgcccgg cgccgcccaa
     5641 gatcgagaac aggatcgcct cggcggcgcg gccctggtgg gtcgggatga tgtgccggaa
     5701 aggcatcagg tcctggacgg cgctctggaa tctgtaaaac gacggcgagc ccgcatagga
     5761 ctcgtcgccg cgcatcagcg cgctccactg ggcggcgctc atcgcgccgg tgccggaatc
     5821 ggtcagcagg tcgaccagca cgtcgtcgga atgcaggccg aacaggttgt aatgcacccc
     5881 ggcgagcagc tccttgcgat cggcccgcgt cgtcaggcgg atcggttcca ccaccttcat
     5941 ccggaagggc tcgattatcg tcttcatggc agtaccccca aggttgtgta gcgtgccaca
     6001 gcaggggggt tggcaatcag gcaaaatagc ggatcctgac cggagtgcca gagcgccatg
     6061 tgtggaatcg tcggagccgt agccgaacgc aatatcgtgc cgatcctgct ggagggcctg
     6121 cgtcgcctcg aataccgggg ctatgactcc gccggcctcg cggtgctcga cgccgggcag
     6181 cacctcgccc gggtgcgcac ggtgggcaag gtcaaggcgc tcgagcaggc gatcgatcag
     6241 cagccggtcg cgggccgggt cggcatcgcc cacacgcgct gggcgacgca cggcgtgccg
     6301 agcgagcgca acgcgcatcc gcacctgtcg cgcgacgggc tcgcgatcgt gcacaacggc
     6361 atcatcgaga accacgagga actgcgcgcc gacctgcagc ggctcggcta cgaattcacc
     6421 tcggagaccg acaccgaggt gatcgcgcac cgcatccatc accacctcgc gagcctcggc
     6481 gatctgttca aggccgtgcg tgccacggtc gccgaactcg agggcgccta cgcgctggcg
     6541 gtggtgagtg ccgcggcgcc cgatcgcgtg atcctcgcgc gcgagggctg cccggtcgtc
     6601 atcggcatcg gcgtcggcga gaacttcgtc gcctccgatg tcgcggcgct gctgccggtg
     6661 acccgccggt tcgtgttcat ggaggaaggt gacgtcgccg aggtgggact cacgagcgtg
     6721 cgcatcatcg accgcgaggg caatctggtc gaacgcgcgg tgcacgaaag cgagctgtca
     6781 gccgacgccg cggagaaggg cccgtaccgt cacttcatgc tcaaggaaat ccacgagcag
     6841 ccgcgcgcgc tcgccaacac gctgcaggag cgcgtcgcca atggccgctt gctcgatgcg
     6901 gcgttcggcc cggccgcaag cgagatcttc cggcgcaccg agcatgtgca cattgtcgcc
     6961 tgcggcacga gctaccacgc cggcgcggtc gcccgttatt tcatcgagca gatctgcaag
     7021 ctgccctgtt cggtcgagat cgcgagcgag taccgctatc gcaatcccgt cgtgctcagg
     7081 aattcgctgt tcgtgacgat ttcgcagtcg ggcgagacgg ccgatacgct ggcggcgttg
     7141 cgcctcgcga agcaggcggg ctatctcgca tcgctcgcga tctgcaacca gcccgagagt
     7201 tcgctggtgc gcgaatcgga cctcgtgatg ctgacgcgcg ccggccccga gatcggagtg
     7261 gcgtcgacca aggcattcac gacccagctg atggcgttgt cgatgctggt cgtcgcgctc
     7321 agcaagtacc atgccggtga tgccgagcgc gagcgcggcc tcatcgcgcg gctgatcgag
     7381 atcccaggcc tgatcgagaa gacgctgacg ctcgacccgg tgatccacca actcgccgag
     7441 cgcttcgccg acaagaacca cgcgctgttc ctcggccgtg gtgcactgca tccaatcgca
     7501 atggaaggcg cgctcaagct gaaggaaatc tcctacatcc acgccgaggc gtatccggcc
     7561 ggcgagctga agcacgggcc gctcgcgctc gtcgacgccg acatgccggt gatcaccgtg
     7621 gcgccgaaca acgacctgct cgagaagctc aagtcgaacc tgatggaggt gcgggcccgg
     7681 ggcggggagc tctacgtgtt cgccgacccc gaagccggct tcgagccctc ggacggcgtc
     7741 accgtgatcc agatgcccaa gcatgtcagc tacttccagg cgccggccgt ctacacgatt
     7801 ccgctgcagc tgctcgccta ccatgtggcg gtcctgaagg gcaccgacgt cgaccagccc
     7861 cgcaatctcg cgaaaagcgt cacggtcgag taacctcggc gttcccccgg gcctgcgggc
     7921 ccctggctgg aggaatgcca tggcgagcga ggagaagccg gaggtcgccg acccgaccaa
     7981 agccgctgcg gagaacccat acacgcagca ctacgttgtg cagaagggcg acacactgtc
     8041 gaagatcgcg cgggagtact acggcgatgc gatgctgtac ccgaagatct tcgaggccaa
     8101 tcgcgacatc ctgtccgatc cgaaccggat caagcctggg cagcgcctgc gcattccctg
     8161 acacattcca ggagtaaatc atgttgaagc gaaccacttg gatcaccgcg atggcggtgc
     8221 tcgtgctggc tgccgcctgc gcgaaccaga agggccccgc gaccaaggcg gtcgcggatg
     8281 ccgagacggc gctggccgcg ttccgcgacg acgcggcgcg cctgctgcct gatgacctgt
     8341 cggccgtcga ttcgaccctc gcgagcctga aggacagcct ggccaagggc gacttcaagg
     8401 ccgtggtcgc cgccgcgccc gacctgtcga gccgcatcac cgcgctgcgc gacgccaccg
     8461 aggcgaagaa ggcggaatgg gaggcggcca atgcggccgc gcgggaacag tggagtgtct
     8521 acgccgccga cctgccgaag atggtcgaag ccatccagag ccgggtcgac atcctcggca
     8581 aggcgcgccg gctgccgcgc aacgtctcgc aggaggcgtt cgacagcgcg aagtccggcc
     8641 tcgactggat gacgacgacg tgggcggaag ccaacgatgc gttcaatgcc ggcaacgcag
     8701 tcgatgcggc tgccaaggcg caggccgtga aggacaaggg caacgaggtg ctcgggttgc
     8761 tgggcctcgc ccccgccgcg tcatgaacac gcgcgagggc gatgccgcca acgacagcgc
     8821 ggccggcgat gatctgcgcg atctgatcgc gcaggccgaa gacatgctgc aatcgctgcg
     8881 cgggcagacg ggggaagccg cggagcgtct gcgtgagcgt gtcgaggcca cggtgacgag
     8941 tgcgcgcaag cgcctctcgt cgctcgagag cgatgcgcgc cagctcaccg aggaggcggc
     9001 cgcgaccgcc gacgagtacg tgcgggacaa tccgtgggcg tcggtggcga ttgccgccgc
     9061 cgccggcgtg atcatcggcg cactgctgtc gcgccgccgc tgagtcgtgg catcgccgac
     9121 gcaacgggtg ctggaggagg cgctcagcct cggcgcgcag ggcctgcgca tcgcgcagac
     9181 ccggctcgag atactgagcg tcgacctgca gcgcgagaag caggcgatcg cgcgccagct
     9241 ggcgctcgcg gtgacctgcg ggacgagcgc ggcgctggcc gcgttcgcct gcatcctgtg
     9301 ggttgcactc gcgttcgagc cgcagacccg cttcatcgtg ctcggcgtgc tgaccggtgt
     9361 actggcgctg gtcgccgtcg cgtgcggcct gctcttctgg cgcggcctgc agcgacgtga
     9421 ccatctgttc gggcaattga tcgaggccct gaaacgtgac agcgacgccc ttgaacctcg
     9481 aagaacgcgc gaaactgctt gaggtcgagg cggagctgca gcgcaggacg ctgcgcgcct
     9541 cgctcgatac gctgcaggcc gccaagggca tcacctgggc gctcgccggg gcgtcgctcg
     9601 cgcgtcggct tgcgatcgtg cacaaggcga aggttgccgg ttatgcgctg atcgggaccc
     9661 ggctgctgtt cagggcgcga cgcgcgcttc gagatcgtaa gctgctgccc cggtgatctc
     9721 gacatcggca aactcgccga ccttgagttt gccgccccgc ctgatccgca ccactccgtc
     9781 gatctcgggc gcgtctcccg gcccgcgcgc gatggcgatc tccctgtcga tgcgatccac
     9841 gagcacgcgc atgcgcgcgc cgatgcgccg cgcgagccgc gacgcgctga tcgcctgcgc
     9901 gcgctccatg aagcgtgcct ggcgttcgag cttgatctcc tcgggcacct gaccgggcag
     9961 tgcgttggcg gcggcgccgt cgaccggcga gtaggtgaag cagccgacgc gatcgagctg
    10021 cgcctcgtcg agccagtcca gcagttcggc gaactcggcc tcggtctcgc cgggaaatcc
    10081 cgcaatgaag gtgctgcgga tcacgagctc cgggcagatc ctgcgccagg cgtggatgcg
    10141 cgccagcgtg tcctcggcgt gcgccggccg tttcatgcgc ttcagcaccg tggggctgcc
    10201 gtgctggaac ggcacgtcga ggtagggcag caccttgcct tcggccatca gcgggatcac
    10261 cgcgtcgacg tggcggtagg gatagacgta gtgcaggcgc acccaggcac cgagctcgcc
    10321 gaggccgcgc gcgagatcca ggaagcgcgt ctcgtaggcc tggccgcgcc attcgcgcgg
    10381 cgcgtagcgg aggtcggcac cgtatgcgct cgtgtcctgc gagatgacca gcagttcctt
    10441 cacccccgcg cgcaccagct gttcgccctc gcgcagcact tcgtcgatgg gtcggctcgc
    10501 gagccggccg cgcatcgacg ggatgatgca gaagctgcac ttgtggttgc agccctcggc
    10561 gatcttcatg taggcgtagt gccgcggtgt cagcttgatg ccctgcggcg gcaccaggtc
    10621 gaggaacggg tcgtgctgcg gcggcaggtg ctcgtgcacg gcaccgatca cgtcctcgta
    10681 ggcgtgcgcc ccggtgacct tcagcacccg cggatggcgt tcgaggatgc gctcggggcg
    10741 ggtacccagg cagccggtga cgattacgcg cccgttctgt tcgatcgctt cgccgatggc
    10801 gtcgagcgat tcatcgatcg cggcatcgat gaagccgcag gtattgacga ccaccacgtc
    10861 ggcatcgtca tagctgggcg cgatcgagta accctcggcc cgcaggtggg tcaggatgcg
    10921 ctcggagtcg accagggcct tcgggcagcc gaggctgacg aaacccacgc ggggtgttcg
    10981 ggtagcaggg ggcatggtgc gaatgatacg cgtgccggca ggaggcccgc cggcctatcc
    11041 tgctgccacc tgctggacgt tgcgtcattc cgctatccgg aattgaccgc ccgttgaacc
    11101 gccccgcgca aatactatcg ccgccgcggg gggacctgcg ggacactggt ggccatgagg
    11161 gatcttcata tcgcggtcgt cggtgccggc atcggcggac tggcggcggc gctcgggctg
    11221 cagcgcgccg ggtttcgggt ctccgtgcac gagcaggcgc ccgagctcgg cgaagtgggg
    11281 gcggggctgt cgctcggccc cacggcggcc catgcgctgc gctggctcgg cctcgggccc
    11341 gcgctcgacc tgcacgccta cctgcccgag aaccaggccg tacgccacta ccaggatggc
    11401 cacgcgctgc agtggatcaa tcgcggccgg tcactgctac agaagtatgg cgaacgttac
    11461 tacctgatcc accgcgccga cgtacatgac gcgctggccg cggcggtgcg cgccaatgat
    11521 ccggacgcaa tccggctggg ccgccggtgc acgggcctcg aacagacggc gaactcggct
    11581 acggtgcgct tcgaagacgg cacgcatgtc acggccgatg tcgtgatcgg tgccgacggc
    11641 tcgcgttcga tcgtgcggcg cctgctgttc ggcgacagcg gacccaccta tacgggctac
    11701 atcgcctggc gcggcctcgt gccgatggag cgcgtgccgc agagcgtgct cgacccgccg
    11761 tcgggcatct atatcgggcc gggccacctc gtgaaccgct acccggtgcg ccgctggcaa
    11821 ctgctgaact tcatcgcctt tgccgagcgt gccgaatggt ccgaggaggg ctggtcgata
    11881 ccttcgacgg tggatgaact gatggccgaa ttcgaggact ggcaccccga cgtgcgcacc
    11941 ttcctcgccg cggtgccgcc cgaactgctg ttcaagtggg gcctgttcga tcgcgagccg
    12001 ctcaccacct ggcacagcgg gcgcacgacg ctgctcggcg atgccgcgca tccggtgttg
    12061 ccgttcctgg gccacggcgc ggtactggcg atcgaggacg cggtgctgct cgcgcgggca
    12121 ttcgccgggt cggccgaaat cggcgaggca ctgcaaaggt acgaggcggc gcgtattcct
    12181 cgggcggcat tcgtcgtgcg cgaatcacgc caggccgtga aggtgtacca ctcgcacgag
    12241 ccggagaagt atgcgcagaa gaccggcgga caggcggccg acgagcggct cggcctgttc
    12301 gactacaacc cctcgacgat ttcagtctga ggcgggctcg gcgcttcctt gcaggagcgc
    12361 agcgcgaaga acgcgagcag cgagaagatg ggcacgagga accacagcca cggcacagcg
    12421 cgtcgttcgg tggagaccat ggtttcagcg cccgtaggta gcgtggattt cggcgacgcc
    12481 gagtgaattc gcgttcacca tgaagccgat cagcgcggca gtcgcgtcgg ccgggacccc
    12541 gagcttctcg accttgagcg cgaacaggcg gaaattgtag cggtgcggcg gatcgccctc
    12601 gggcgggcag gcgccaccgt agccagggat gccgaagtcg gtgcgcccgt gtaccgcgcc
    12661 cgggggcagt tgcctgccat cgcttgcgcc ggcgccggcg gacaggctgc gcacgtcggc
    12721 cggtatgtcg tagacgaccc agtgccacca gccgctgccg gtgggagcat cgggatcgtg
    12781 caccatcagc gcgaagctct tggtgccctc cgggaccccg gaccaggcca gggcgggcga
    12841 gacattgccg ccggagcagc cgaagctgtt gaagacctgt tcatcggcca gggtgccgcc
    12901 cggtgcaaaa ttgcccgagg tcagggtgaa cgcggcgggc ccggccaccg tggcggtggt
    12961 gaatccgagg ctcgcgccga gcagcacgca caaggtggag acacgaatca tggtggcgag
    13021 tttgccagtg gcgcccggta tagtcacgac ctccatgacc ggccgcttcg atcgcgccta
    13081 ttacgagcgc tactacaacg atccgcgcag cagggtgacc tcgcgcgccg agatgagcgc
    13141 gcgcgggcgg ctgatcggcg ccttcgcact ttacctggac ctgccggtgc gccgtatcct
    13201 cgatgccggc tgcggcgtgg gcctgctgca ggcgccgctc aggcggatgc tgccgaaggc
    13261 gacctatacc ggcatcgagg tcagcgacta cctgtgccgc aggcacggct gggtgcaggc
    13321 ctcgctcg
//
LOCUS       DAOQVY010000061        13277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_5266_length_13277_cov_6.037135, whole genome shotgun sequence.
ACCESSION   DAOQVY010000061 DAOQVY010000000
VERSION     DAOQVY010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13277
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5266_length_13277_cov_6.037135"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1568
                     /locus_tag="PKN91_07195"
     CDS             <1..1568
                     /locus_tag="PKN91_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS27892.1"
                     /translation="TWLPWVGALLAAKFLLSWLYRTAQYRASSRTKLTVRDRIYEHAL
                     RLGPAVLDRKRTGELVNIAVDGMDWIEMYYGIYFVQFVIGMATPLLLCLFIGFTDWVV
                     GVALLVSVPLTPLFLGMMARNFRKASERFAQVNAEQGARFLDSIQGMTTLKMFNLGKV
                     RGAEMHAASEQQRVETMRLLLVNQMMILFVDFDFALGTTLVLTVTALLRLDAGALTPG
                     AVVALILASAEFARPLTLVGQFFFAGAIGRAYAKKIAAFLGEQPGVADPTGVAPPPLE
                     ARPVLSLRNIVFRFAGASRPAVDGLSLDIVPGETVALVGASGSGKTTVTNLILRTIAP
                     DAGELALGGRNVASVPADWVRAHLALVPQDPYLFYGTIAENLRVAKPDASDDELVAAC
                     RAASIHEHIAALPQGYATRVGERGLSLSGGQVQRLAIARALLKDAPIVILDEPTSQID
                     LETEAVIHDALGHLTRDRTVLLIAHRLSTVARADRIVVMGDGKVLESGSHPELIARGG
                     VYADMVAIGGSAA"
     gene            1565..3379
                     /gene="cydC"
                     /locus_tag="PKN91_07200"
     CDS             1565..3379
                     /gene="cydC"
                     /locus_tag="PKN91_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HNS27893.1"
                     /translation="MSKLQLLRRLLAFMRPLNGVMLVSVTARTVKLLMQTALIAFAAA
                     SVARHVADPAAGAIGHVVLGLMGYALLLGLSNYIETYTGHFVAFRLLSMLRNEFYDRM
                     EPLAPAGTGQLRSGDAVSRVVSDCERIEPFYAHTIAPVITAFVVPAVLLWILGHYVHP
                     TYAWTLLPFMLAMALVLPVVTALSGGRGSEPWRRAQGEVNAALTDSLQGIRDTVAFGY
                     GDRRRRLMWEIGERLKKGQDAMTRADALQRGVTELVIATAAIATLWVSWDLYQAGAIE
                     PLRDIPIAVAIAITGFNAPQAMNNVVGDFQVAMISARRLFELMNQKPAVENLVTSSPP
                     PADASLALRNVSFTYDPAAQSAAPVLRDISFEVEAGRHVALVGSSGAGKSTIANLLLR
                     FWDATEGTVLVGGRPVRDYTLEDLRGHIAVVSQRNYTFNTTIGENIRMGRPDATLAEV
                     DEAARRAQLADWIASLPLGYETQVGEMGSKISGGQRQRIAIARALLKDAPILVLDEAT
                     SNLDVESEREVNAAIRELAQGRTTLTIAHRLSTVLSADEILVLEHGRIVERGTHATLL
                     AQRGVYARLFELQQDEVDMRIAGPAAQAARGTSGPAIP"
     gene            3435..3818
                     /locus_tag="PKN91_07205"
     CDS             3435..3818
                     /locus_tag="PKN91_07205"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HNS27894.1"
                     /translation="MRKLVLVVVGLVFTGLALLGAVLPGLPTTPFLIVALWAFARSSR
                     RLTLWLERVPILQSALRDAQRFEERRELRLTVKLAAIALAWGSVLFVAWWKRSLTEPL
                     VGVMVLGAIAGTVTMILIPTDREPR"
     gene            complement(3820..5238)
                     /locus_tag="PKN91_07210"
     CDS             complement(3820..5238)
                     /locus_tag="PKN91_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNS27895.1"
                     /translation="MIDPASLEFTPRDMRRMGDAVMARVVDHLATIGEQPVRGDMDAA
                     DLCRAMREPAPEQGSALEPLLDSLFDEWIPRSFNTASPGYLAYVPGGGVFPGALADLI
                     ASGVNRYTGVWPTAPALVQLEANVLDWLRDWMQLPATTRGLFTTGGSMANFAAIVCAR
                     ERNLGPELRRGTMYLSTETHHSVGKSAKLAGILPDRVRTIEVDGAFRMRTDLLEQAIA
                     RDRAAGLVPFLVVSSAGSTNTGAVDPLRDIAALCRREGLWHHIDGAYGASFHLCPELR
                     PLLAGLPEADSLTLDPHKGLFLPYGTGAVLVRDGETLRAAHAQTASYMPESHEDEFYD
                     PHQYGPDLSRGFPGLRVWLAFKLFGAARYRAALAEKRALALDAARRIAALPQVVMDAP
                     PQLSLFAFHLEWPGATRAEQDAATRELLERVTRRGRVFITGCHAGGRFLARVCVLSFR
                     TRTAQIDTCVEHIAEEARALCP"
     gene            complement(5235..6179)
                     /locus_tag="PKN91_07215"
     CDS             complement(5235..6179)
                     /locus_tag="PKN91_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNS27896.1"
                     /translation="MAQWGLLLTLLIVVLSAFVRQRGVGLGCEDWPACYGQDLARTQS
                     GEVVAAGEDVLLARLAHRVVASVTLVLVIAMVLAALGARPALRRAGTLALAMLAIALG
                     LAVLGVVTPGSRLPAVALGNLLGGFAMFALSARLVTVTGTSRPADAMLARAAILVAIL
                     LVLQVTLGAMVSATHSGLSCRALAECLDLTASGGWNFALLDPWRLPPPGSIASGAAGA
                     PLQLAHRVGSIVAAAAVLALSLLAWRRGRRRRALALAGLLLGVVTLGLFFVGAGKLPL
                     PAVLLHNLGAVSLLALVGVTGLGPAASAIGVGRQALRQ"
     gene            complement(6238..8127)
                     /locus_tag="PKN91_07220"
     CDS             complement(6238..8127)
                     /locus_tag="PKN91_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNS27897.1"
                     /translation="MIGRLQALLRWLFMHVEALFNRAFGDQLNPFYHLGSIAFFLFWI
                     IAGSGLYLYAFFDTSVVGAYSSVEALTHRQWFAGGVLRSIHRYASDAMVVVMMIHLMR
                     HFAFDRMRGFRSFSWLTGVALIWLVYTAGVNGFMLPWDRLAQFVIDGSFEWLARLPGF
                     DALIRNVLYPASINDRFFSLLVFIHIGVPLLSLLLMLVHVQRVPKARMQPPRVIALSV
                     TLMLLALSLVLPVLSQGGAADLATAVTALEFDWFYLGGLALLYRWSPDRVWLLTGALT
                     ALLVVIPWLPLRRRGAQRRFQLTIHPGPGHASARAGETILEAGLRAGVALRYECRNGG
                     CGECLCTVLNGSVDHGAYQPSVLTQAMRDAGKALMCCATPRSDLEIEVEALAADATSS
                     VRSYVGQVASLEKLSPDVMRVRISLPGRHRVDFTAGQYINIVLEDGQRRAFSFANTPQ
                     DNEYIELHVRLIPGGRFTTHVFTAMQVGDSVRFEGPFGRFTLREGPKPILYVAGATGF
                     APIKSIVEDAFRRGVDRPMHLYWGVRERKDLYMLELVERWAREHPNFRVTPVLSEESS
                     SDWSGRRGLVHEAMLADYPDLSGHEVYVCGSVRMVETAVPAFISRGLSEDACVSDAFL
                     PSGGV"
     gene            complement(8124..9575)
                     /locus_tag="PKN91_07225"
     CDS             complement(8124..9575)
                     /locus_tag="PKN91_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNS27898.1"
                     /translation="MNGAAEAPLDTPLRAVRPTASSLDDYLLVVPEGAQRSLARGWLW
                     LALLALVGAGLFSVLLVLSRTPVINEWFPVADFFRLALVVHVDLSVLVWFVAIAGLLW
                     TINGSERGLGWAWAALALCVAGAALMSLSPFFGRGEAVMANYIPVLDDRWFIAGLVVL
                     ALGVAVLVLRSLIAAPRLGVTFGGAGALRFGLNASVVSTAVALLAFGWSFAVMPAGLG
                     GKAYYEILFWGGGHALQFTWTLLMLVGWLWLASAGGARVPLSPRVSLLMFTLALASVF
                     VTPYAYLAHDIASVEHRDLHTWAMRFGGGLSILPVSLAVVIALGADVVARRRQSAVQA
                     PLRAALICSVLLFAAGGVIGVFINGNNVRIPAHYHGSTVGVTLALMGLVYYLLPQLGY
                     RAVSGRLAVLQPFLYGAGQLMHIIGLVWSGGYGVQRKVAGTEQVLGSTAEVFGMGLMG
                     LGGLVAIIGGVLFIVVVAGSMRHRGGAPAGLTP"
     gene            complement(9572..10075)
                     /locus_tag="PKN91_07230"
     CDS             complement(9572..10075)
                     /locus_tag="PKN91_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27899.1"
                     /translation="MSKVFSWAGQAMLYVAFAVVIGVFTRWPSYQHLPEDHALVKLSL
                     IHHGQRLEACRRLDPEELAKLPPNMRAPVSCPRERSPILVEVDVDGVTVLRQTAQPAG
                     MSRDGAASVYQRFEVPAGTHRFDVRLRDSARTEGFDFQLSDTVDLRPAQVLVIDFSAE
                     KGGITLQ"
     gene            complement(10072..11616)
                     /locus_tag="PKN91_07235"
     CDS             complement(10072..11616)
                     /locus_tag="PKN91_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNS27900.1"
                     /translation="MMREPALAAWNYCERFLDALFGGALNPLRHLGALASLFFLLLAV
                     SGVYLYAVLDTSLEGAWRSIDDLSRNQWWLGGVFRSLHRYAADAFVIVTALHLLREWL
                     LGHYRGFRRYSWLTGVPLLMFIWISGVGGFWLNWDRLGQFSAIATAELLDWLPVFGSA
                     FARNFLDDAAMSDRLFSLFVFVHIGVALLLLFTAWFHLHRISHAEVLPSRALAAGASA
                     TLLLLALVAPVMSQAPADLSVAQGTLAIDWLLLFMLPVMYATSPGWVWALSAGALLLL
                     FALPFATRAARPEVARVDADNCNGCRQCFDDCPYAAITMAPHPDGKPGNEIAIVDASL
                     CASCGICTGACPPASPFRSVEPLTPGIDMPQAPIGALRERLEARLAALHGDSRIVVFG
                     CDHGANVGALASADVIDFSLLCTGMLPPSFIEYAARHGATGVLITGCREGGCEFRLGN
                     RWTVERLSGERKPWLRRRAPRERLRVVWADRGEEKSLQAAVRDLRNLPPAAVAGAIRR
                     PARGVR"
     gene            complement(11613..12506)
                     /locus_tag="PKN91_07240"
     CDS             complement(11613..12506)
                     /locus_tag="PKN91_07240"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNS27901.1"
                     /translation="MPVRWRPPLPVLLLAALGSGGWTPLWAVHEASGSNPLVADLPTG
                     AITTPGLDGEAALRASQAAVGRMPDDYTLRDRQGRPVQLSSFRGRPLLVSFIYTGCYH
                     ICPASTQVLHQAVRAMHRRFGDRQFNVVTIGFNQPEDTPAAMQEFARQRRISDPNWEF
                     LSPDAADVAALARDFGFTFAATRGGFDHILQVTMLDAQGRVYRQVYGDSFTAERLGEP
                     LKQLLTGARIVDGGVGITDLIDRVRILCSVYDPKSGTYRADYTLYFMIAGGVTFFLAM
                     LWFALSEWRSHLLARRSVNRG"
     gene            complement(12586..>13277)
                     /locus_tag="PKN91_07245"
     CDS             complement(12586..>13277)
                     /locus_tag="PKN91_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNS27902.1"
                     /translation="KAPWGNPTFSGVFISIIGFGFLGGISGVMMGTEQLNMIIHNTIY
                     VPGHFHATVVVGTTLSFMALTYYLIPVLFKREMIAPRLAKWQPYLFGLSMYFFCLVMM
                     GAGTLGVSRRHWDMAFDGSALAYEWPGAAYMMMGLVGISGIAAIVGGAIYIYVTVGSL
                     LWGKRLEGGAKSDKVTPIPPTAPAAAAQTYGSMGFVAPGTFVLAVVFLIAFVLYYFIN
                     WKYLSTLWGLS"
BASE COUNT         2364 a         4615 c         4441 g         1857 t
ORIGIN      
        1 cgacgtggct gccctgggtg ggcgcgctgc tcgcggccaa gttcctcctt agctggctgt
       61 accgcaccgc gcagtaccgc gcctcctcgc gcaccaagct gacggtccgc gaccgcatct
      121 acgaacatgc gctgcggctc gggccggcgg tgctcgatcg caagcgcacc ggcgaactgg
      181 tcaacatcgc ggtcgacggc atggactgga tagagatgta ctacggcatc tacttcgtcc
      241 agttcgtgat cggcatggcg accccgctgc tactgtgcct gttcatcggc ttcaccgact
      301 gggtcgtcgg cgtcgcgctg ctcgtcagcg taccgctcac gccgctgttc ctcggcatga
      361 tggcgcgcaa cttcaggaag gcgagcgagc gcttcgcgca ggtcaatgcc gaacagggcg
      421 cccgtttcct cgattcgatc cagggcatga cgaccctgaa gatgttcaac ctcggcaagg
      481 tccgcggcgc cgagatgcac gctgcgagcg agcagcagcg cgtcgagacg atgcgcctgc
      541 tgctcgtgaa ccagatgatg atcctgttcg tcgacttcga ctttgcgctc ggcaccaccc
      601 tggtgctgac ggtcaccgca ctgctgcgtc tcgacgccgg cgcactgacg ccgggggcgg
      661 tggtcgcgct gatcctcgcg agcgccgagt tcgcgaggcc gctcacactc gtcggtcagt
      721 tcttcttcgc cggtgcgatt ggtcgcgcct atgcgaagaa gatcgccgcc ttcctcggcg
      781 agcagccggg agtcgccgac ccgaccggcg tcgccccgcc gccgctcgaa gcgcggccgg
      841 tcctgtcgct caggaacatc gtgttccggt tcgcaggcgc ctcgcgcccg gccgtcgacg
      901 gcctctccct cgacatcgtg ccgggcgaga ccgtcgcgct ggtcggcgcc agcggctcgg
      961 gcaagaccac cgtaaccaac ctgatcctgc gcacgatcgc acccgacgcc ggcgaactgg
     1021 ccctcggcgg gcgcaacgtc gcttcggtgc ccgccgactg ggtgcgcgcg cacctcgcgc
     1081 tcgttccgca ggatccctac ctcttctacg gcacgatcgc cgagaacctg cgtgtcgcga
     1141 agcccgacgc aagcgatgac gaactggtcg cggcctgtcg cgccgccagc atccacgagc
     1201 acatcgcggc gctgccacag ggctatgcga cccgcgtcgg cgagcgcggc ctgtcgcttt
     1261 ccggcggcca ggtgcagcgc ctcgcgatcg cacgtgcgct gctgaaggac gcgccgatcg
     1321 tgattctcga tgaacccacc tcgcagatcg atctcgagac cgaggcggtg atccacgatg
     1381 ccctcgggca cctcacgcgc gacaggaccg tgctgctgat cgcgcatcgc ctcagcaccg
     1441 tcgcgcgcgc cgaccgtatc gtcgtgatgg gcgacggcaa ggtgctcgag tccggctcgc
     1501 acccggaact gatcgcgcgt ggcggcgtct acgccgacat ggtggcgatc ggcgggagcg
     1561 cggcatgagc aagctgcaac tgctgcgccg cctgctcgcc ttcatgcggc cgctgaacgg
     1621 cgtcatgctg gtttcggtca ctgcgcgcac cgtgaagctg ctgatgcaga cggcgctgat
     1681 cgcgtttgca gcggccagcg tggcgcgcca cgtcgcggac ccggctgccg gcgcgatcgg
     1741 ccatgtggtc ctcggcctca tgggctatgc cctgctgctc ggcctgtcga actacatcga
     1801 gacctacacg ggccacttcg tcgcttttcg cctgctgtcg atgctgcgca acgagttcta
     1861 cgaccgcatg gaaccgctgg cgccggcggg cacgggccag ctgcgctcgg gagacgccgt
     1921 ctcgcgcgtc gtcagcgatt gcgagcgcat cgagccgttc tacgcgcaca cgatcgcgcc
     1981 ggtgatcacg gccttcgtcg tgccggcggt cctgctgtgg atcctgggcc actacgtcca
     2041 cccgacatac gcctggaccc tgctgccgtt catgctcgcg atggcgctcg tgctgcccgt
     2101 ggtcaccgcg ctgtcgggcg gccgcggcag cgaaccgtgg cgccgggcgc agggcgaagt
     2161 gaacgccgcg ctgaccgaca gcctgcaggg catccgcgat accgtcgcct tcggctacgg
     2221 cgaccggcgc cgcaggctga tgtgggagat cggcgagcgc ctcaagaaag gccaggacgc
     2281 gatgacgcgc gcagacgccc tgcaacgcgg tgtgacggag ctggtgatcg cgacggcggc
     2341 catcgccacg ctgtgggtca gctgggatct ctaccaggcc ggcgcgatcg agccgctgcg
     2401 cgacatcccg atcgccgtcg ccatcgccat taccggcttc aatgccccgc aggcgatgaa
     2461 caacgtggtc ggcgatttcc aggtcgcgat gatttcggcg cgacgcctgt tcgagctgat
     2521 gaaccagaag ccggccgtcg agaacctggt cacgagctcc ccgccgccgg ccgacgcctc
     2581 gctggcgctg cgtaacgtga gcttcacgta cgacccggcg gcacagtccg ccgcgccggt
     2641 gctgcgcgac atcagcttcg aggtcgaagc cggccgccac gtcgcgctgg tcgggtcgag
     2701 cggcgcgggc aagagcacga tcgccaacct gctgctgcga ttctgggatg cgaccgaagg
     2761 cacggtcttg gtcggcgggc ggcccgtgcg cgactacacc ctcgaggacc tgcgcggcca
     2821 cattgccgtc gtctcgcagc gcaactacac attcaacacg acgatcggcg agaacatccg
     2881 catgggccga cccgacgcca cgctggccga agtcgacgag gccgcacgac gtgcgcagct
     2941 tgcggactgg atagccagcc tgccgctcgg ctacgagacg caggtcggcg aaatgggcag
     3001 caagatctcg ggcggacagc gccagcgcat cgcgatcgcg cgcgcactgc tgaaggatgc
     3061 gccgatcctg gtcctcgacg aggcaacctc gaatctcgac gtcgaatcgg aacgcgaggt
     3121 gaatgccgcg atccgcgaac tcgcgcaggg acgcaccacg ctcacgatcg cgcaccgcct
     3181 gtcgaccgta ctcagcgccg acgagatcct ggtactcgag cacggccgca tcgtcgagcg
     3241 cggcacgcat gcgacgctgc tcgcgcagcg cggcgtctat gcgcgcctgt tcgagctgca
     3301 gcaggacgag gtggacatgc gcatcgccgg tcccgccgcg caggccgcgc gcggcaccag
     3361 cggccctgca ataccgtaga cttgatggcc cgcaaaccag ggattggaaa ggcagcaact
     3421 acgggagcct ctcgatgcgc aagctggtgc tggtcgtcgt cggcctggtg tttaccgggc
     3481 tggcactgct cggcgcggtg cttcccggcc tgcccaccac gcccttcctg atcgttgcgt
     3541 tgtgggcgtt cgcgcgatcg tcccggagat tgactctctg gctcgagcgc gtaccgatcc
     3601 tgcaatcggc attgcgcgat gcacagcgat tcgaggaacg ccgcgagctg cgcctcacag
     3661 tcaagcttgc ggcgatcgcc ctcgcctggg gttcggtcct gttcgtggcc tggtggaagc
     3721 gctcgctgac cgaaccgctg gtcggcgtca tggtgctggg tgcgatcgcg gggaccgtga
     3781 cgatgatatt gattccgacg gaccgggaac ctcgctagct cagggacaaa gcgcccgcgc
     3841 ctcctcggcg atgtgctcga cgcatgtatc gatctgcgcc gtgcgcgtgc gaaagctgag
     3901 cacgcatacg cgcgcgagga aacggccgcc ggcgtggcag ccggtgatga acaccctgcc
     3961 gcggcgcgtg acccgctcga gcagctcgcg cgtggccgcg tcctgctccg cgcgcgtcgc
     4021 accgggccac tcgagatgga acgcgaacag cgacagctgc ggcggggcgt ccatcaccac
     4081 ctgaggcagc gccgcgatgc gccgcgccgc atcgagcgcg agcgcgcgct tctcggcgag
     4141 cgcggcccgg tagcgcgccg cgccgaacag cttgaacgcg agccacacgc gcaggcccgg
     4201 gaagccgcgc gagaggtcgg ggccgtactg gtgcggatcg tagaattcgt cttcgtggct
     4261 ctcgggcatg tagctcgcgg tctgcgcatg ggcggcccgc agcgtctcgc catcgcggac
     4321 gagcacggcg ccggtgccat agggcaggaa cagtcccttg tgcgggtcga gcgtgagcga
     4381 gtcggcctcg ggcagcccgg cgagcagcgg acgcagctcc gggcacaggt ggaaactcgc
     4441 gccgtacgcc ccgtcgatgt gatgccacag cccctcgcgc cggcagagcg cggcgatatc
     4501 gcgcagcgga tcgactgcac cggtgttggt gctaccggcg gaggacacga caaggaacgg
     4561 cacgaggccc gctgcgcgat cccgcgcgat cgcctgctcg agcaggtcgg tgcgcatgcg
     4621 aaacgcgcca tccacctcga tggtgcgcac acggtcgggc aggatgcccg cgagcttcgc
     4681 ggacttgccg accgagtggt gcgtctcggt ggacaggtac atggtgccgc ggcgcagctc
     4741 gggaccgaga ttccgttccc gtgcgcagac gatcgcggcg aaattcgcca tcgagcctcc
     4801 ggtcgtgaac agtccgcgcg tggtggcggg cagctgcatc cagtcgcgca gccagtcgag
     4861 gacgttcgct tcgagctgca ccagcgccgg cgcggtaggc cacacgccgg tgtagcgatt
     4921 gacgccggag gcgatgaggt cggcgagtgc gccgggaaac accccgccgc ccggtacgta
     4981 ggcgaggtag ccgggactcg cggtattgaa actgcgcggg atccactcgt cgaacagcga
     5041 atcgagcagc ggctccaggg ccgacccctg ttcgggcgcc ggctcgcgca tcgcgcggca
     5101 caggtccgct gcgtccatat cgccgcgcac gggctgctcg ccgatcgtcg cgaggtggtc
     5161 cacgacacgc gccatgaccg cgtcgcccat gcggcgcatg tctcgcggcg tgaactcgag
     5221 gctcgcgggg tcgatcattg tcgcagggct tgccgcccaa ccccgattgc cgatgccgcg
     5281 ggtccaaggc cggtcactcc aaccaatgcc agcaggctga ccgcgcccag gttgtgcagc
     5341 agcaccgcgg gcaggggcag cttgcccgca ccgacgaaga agaggccgag cgtcaccacg
     5401 cccagcagca gaccggccag cgccagcgca cggcggcgtc ggccgcgccg ccacgcgagc
     5461 aggctgaggg cgagcactgc cgcggccgcc acgatggatc ccacccggtg ggccagctgg
     5521 aggggtgctc cggcagcgcc gctcgcgatg ctgcccggcg gcggcagccg ccacggatcg
     5581 agcagcgcga agttccagcc gccgctcgcc gtcagatcca ggcactcggc cagtgcgcga
     5641 cagctcagcc ccgagtgcgt cgcactcacc atggcaccaa gggtgacctg cagcaccagc
     5701 aggatcgcaa ccaggatcgc cgcgcgggcc agcatggcat cggcgggtcg ggatgtaccg
     5761 gtcaccgtga ccagccgcgc agacagcgcg aacatcgcga aaccacccag cagattgccc
     5821 agcgccacgg cgggcaaccg ggaacccggg gtcaccacgc cgagcaccgc gagcccgagg
     5881 gcgatcgcga gcatggcgag cgccaaggtg cccgcgcgcc gcagcgccgg acgggccccg
     5941 agggcggcga gcaccatcgc gatcaccagc accagcgtca cagaggccac cacccggtga
     6001 gccagccgcg cgagcaggac atcctccccg gcggcaacca cctcacccga ctgcgtgcgg
     6061 gccaggtcct ggccatagca ggcaggccag tcctcgcagc cgaggcccac tccgcgctgc
     6121 ctcacgaacg cgctcagcac cacgatcagc agggtcaaca gcaggcccca ctgcgccatg
     6181 cgtcgcagcg tcccgcggcg tagttcgggg tcgactggcg cgctggccat ggtctgccta
     6241 gaccccgccc gacggcagga aggcatccga cacgcaggcg tcctcgctga gtccacgcga
     6301 gatgaacgcc ggcaccgccg tctcgaccat gcgaaccgag ccgcacacgt atacctcgtg
     6361 gccgctcagg tccggatagt ccgccagcat cgcttcgtgc acgagcccgc gccgcccgga
     6421 ccagtcgctc gagctttcct cggagagcac cggggtcact ctgaagttcg ggtgctcgcg
     6481 cgcccagcgc tcgaccagct cgagcatgta gaggtccttg cgttcgcgca cgccccagta
     6541 caggtgcatc ggccgatcga caccgcgccg gaacgcgtcc tcgacgatgc tcttgatcgg
     6601 ggcgaagccg gttgcgcccg cgacgtacag gatcggcttc ggcccctcgc gcagcgtgaa
     6661 gcggccgaat ggtccctcga agcgcacact gtcgccgacc tgcatggccg tgaacacatg
     6721 cgtcgtgaaa cgcccaccgg ggatcaggcg cacgtgcagt tcgatgtact cgttgtcctg
     6781 cggcgtattg gcaaacgaga atgcccgccg ctgcccgtcc tcgagcacga tattgatgta
     6841 ctggccggcg gtgaaatcga cgcggtgccg accagggagc gaaatcctga ctcgcatcac
     6901 gtcaggggac agtttctcga ggctcgccac ctgcccgaca taggagcgca cactgctcgt
     6961 ggcatcggcg gcgagtgcct cgacttcgat ttcgaggtcg ctgcgtggcg tcgcgcagca
     7021 catcagtgcc ttgcccgcat cacgcattgc ctgcgtcagt acgctcggct ggtaggcgcc
     7081 atggtccacg ctgccgttga gcacggtgca caggcactcg ccgcagccgc cgttgcgaca
     7141 ctcgtagcga agcgccactc cagcccgcag gccggcctcg agaatggtct cgcccgcgcg
     7201 cgcgctcgca tggcccgggc ccggatggat cgtgagctgg aacctgcgct gggcgccgcg
     7261 gcgcctcagc ggcagccatg gaatgacgac gagcagggcc gtgagggctc ccgtgagcag
     7321 ccacaccctg tcgggcgacc agcgatacag cagcgcgagg cccccgaggt agaaccagtc
     7381 gaactcgagc gcagtgaccg cggtggcgag gtcggccgcg ccgccctggc tgagaaccgg
     7441 cagcaccagc gagagggcga gcagcatgag cgtgacgctg agcgcgatca cccgcggcgg
     7501 ctgcatgcgc gccttgggaa cgcgctgcac atgcaccaac atcagcagca gcgacagcag
     7561 cggcacaccg atgtggatga acaccagcag tgaaaagaaa cggtcgttga tgctcgctgg
     7621 atagaggacg ttgcggatca gcgcatcgaa ccccggcagg cgggcgagcc attcgaaact
     7681 gccgtcgatg acgaactggg ccagcctgtc ccacggcagc atgaagccgt tgacgcctgc
     7741 ggtgtagacg agccagatca gcgccacgcc ggtcagccac gagaacgacc tgaaaccccg
     7801 catgcgatcg aatgcgaagt ggcgcatcag gtggatcatc atcacgacca ccatcgcgtc
     7861 ggatgcgtag cgatggatgc tgcgcagcac gccaccggcg aaccactgcc tgtgggtgag
     7921 cgcctcgacc gaactgtagg caccgaccac actggtgtcg aagaacgcgt agaggtagag
     7981 gccgctgccg gcgatgatcc agaaaaggaa gaatgcgatc gaaccgaggt ggtagaaggg
     8041 gttcagctga tcgccgaagg cgcggttgaa cagcgcctcg acatgcatga acaaccagcg
     8101 cagcagcgcc tgcagcctgc cgatcatggc gtcagccccg ccggcgcacc gccgcggtgg
     8161 cgcatcgagc cggcgacgac cacgatgaac agcacgccgc cgatgatcgc aacgaggccg
     8221 ccgagtccca tcagtcccat gccgaagact tcggcggtgc tgccgagcac ctgctcggtc
     8281 ccggcgacct tgcgctgcac gccgtaaccg cccgaccaga cgaggccgat gatgtgcatg
     8341 agctgcccgg caccgtaaag aaacggttgc agcacggcga ggcgcccgct caccgcgcga
     8401 tagcccagtt gcggcagcaa gtagtagacg aggcccatca gcgccagcgt cacgcccacg
     8461 gtgctgccgt ggtaatgcgc ggggatcctc acgttgttgc cgttgatgaa cacgccgatc
     8521 acgccgcccg cggcgaacag cagcaccgag cagatcagcg cggcgcgcag cggtgcttgc
     8581 accgcgctct gccgccggcg cgccacgacg tcggcgccga gcgcgatcac gaccgccaga
     8641 ctgaccggca ggatggacag gccgccgccg aatcgcatcg cccaggtatg caggtcgcgg
     8701 tgctccacgg aggcgatatc gtgcgcgaga taggcgtaag gggtaacgaa cacgctcgcg
     8761 agcgccagcg tgaacatcag cagactcacc cgcgggctca atggcacgcg tgcaccaccg
     8821 gcgctggcga gccacaacca gccgaccagc atcagcagtg tccaggtgaa ctgcagcgcg
     8881 tggccgccac cccagaagag gatctcgtaa taggctttgc cgccgaggcc cgccggcatc
     8941 accgcgaacg accagccgaa cgccagcagc gccacggccg tggacaccac gctcgcgttg
     9001 aggccgaaac gcaacgcccc ggcaccgccg aatgtcacgc cgagcctggg tgcggcgatg
     9061 aggctgcgca gcaccagtac cgcgacgccc agcgccaaca cgaccagtcc cgcgatgaac
     9121 cagcggtcgt cgagcacagg gatgtagttg gccatgacgg cctcaccccg accgaaaaac
     9181 ggtgacagcg acatcagtgc ggcgccggcg acgcacagcg cgagcgcagc ccacgcccag
     9241 ccgagccccc gttccgagcc gttgatggtc cagagcaggc cggcgatcgc gacgaaccag
     9301 accagcaccg acaggtccac gtgcaccacc agcgccaggc gaaagaagtc cgccaccgga
     9361 aaccattcgt tgatcaccgg cgtgcgcgac agcaccagca gtaccgaaaa gagcccggcg
     9421 ccgaccaacg ccagcagcgc gagccacaac cagccgcgtg ccagcgatcg ctgcgcaccc
     9481 tcggggacca cgagcaggta gtcgtcgagc gaggatgcgg tcgggcgcac tgcgcgcagc
     9541 ggcgtgtcca gcggggcttc tgcggcgccg ttcattgcag cgtgatcccg cccttttcgg
     9601 cgctgaagtc gatcacgaga acctgggccg gcctcaggtc gaccgtgtca ctgagctgga
     9661 agtcgaaacc ctcggtacgc gcactgtccc tcaatctgac gtcgaaacgg tgggtgccgg
     9721 cggggacttc gaagcgctgg tacaccgagg ccgcgccgtc gcgggacatc ccggcaggtt
     9781 gcgccgtctg gcgcagtacg gtgacgccgt cgacgtcgac ctcgaccagg atgggcgaac
     9841 gttcccgcgg gcagctcacc ggcgcacgca tgttcggcgg cagtttcgcg agctcctccg
     9901 ggtccagccg ccggcatgct tccagtcgct ggccgtggtg gatcaagctc agtttgacga
     9961 gcgcgtgatc ctccggcagg tgctggtagg acggccagcg cgtgaacacg ccgatcacca
    10021 ccgcgaatgc gacatacagc atcgcctgcc cggcccatga gaacactttg ctcatcgcac
    10081 tcctcgcgcg ggtcggcgga tcgcaccggc cacggcggcg ggtggcaggt tgcgcaggtc
    10141 gcgcacggcg gcctgcagcg acttctcctc cccgcgatcg gcccatacga cccgcagccg
    10201 ctcgcggggc gcgcgcctgc gcagccaggg tttgcgctcg ccactcagac gctccacggt
    10261 ccaccgattg ccgaggcgga actcgcagcc gccctcgcgg cagcccgtga tcagcacgcc
    10321 ggtggcgccg tgccgcgctg catattcgat gaacgacggc ggcagcatac ccgtacagag
    10381 caggctgaag tcgatcacat cggcactcgc cagcgcaccc acgttcgcgc cgtggtcgca
    10441 accgaagacc acgatccggc tgtcgccatg caatgccgcg aggcgcgcct cgagccgttc
    10501 acgcaaggcg ccgatcggcg cctgcggcat gtcgatgccc ggcgtcaggg gctcgacact
    10561 gcgaaacggc gacgcgggcg ggcaggcgcc cgtgcagatg ccgcaactcg cgcacagcga
    10621 agcatccact atcgcgatct cgttgcccgg cttgccgtcc ggatgcggcg ccatcgtgat
    10681 ggccgcgtac gggcaatcgt cgaaacattg ccggcagccg ttgcagttgt cggcatccac
    10741 ccgggcgacc tcgggccgtg cggcgcgcgt tgcgaacggc agcgcgaaca acagcagcag
    10801 tgcgcccgcc gacagggccc agacccagcc gggcgaggtg gcatacatca ccgggagcat
    10861 gaacagcagc agccagtcga tggcgagcgt gccctgcgcc accgagagat cggccggtgc
    10921 ctgactcatg acgggcgcaa ccagtgccag cagcagcaat gtggccgaag ccccggccgc
    10981 aagcgctcgc gacggcagca cttcggcgtg cgagatgcgg tgcaggtgaa accacgccgt
    11041 gaacagcagc aacagcgcta cgccgatatg gacgaacacg aacagcgaga acaggcgatc
    11101 gctcatggcc gcgtcgtcga ggaaattgcg cgcgaatgcc gaaccgaaga ccggcagcca
    11161 gtcaagcagc tccgccgtgg cgatcgcgga aaactgcccg agccggtccc agttcagcca
    11221 gaagccgccg acaccgctga tccagatgaa catcaacagc ggcactccgg tcagccacga
    11281 atagcgccga aagccgcggt aatgcccgag gagccactcg cgcagcaggt gcagtgcagt
    11341 gacgatcacg aaggcgtcgg ccgcatagcg atgcaggctg cggaacaccc cgccgagcca
    11401 ccactggttg cgggacagat cgtcgatgga gcgccaggca ccctcgagcg aagtatcgag
    11461 caccgcgtag agatagaccc cgctcaccgc gagcagcaga aagaacagcg aggccagcgc
    11521 accaaggtgc cgcagcggat tcagggcgcc gccgaacagg gcgtccagga accgttcgca
    11581 gtaattccag gccgcgaggg ccggctcacg catcatccgc ggttcactga ccgacgggcg
    11641 agcaagtggc tgcgccattc cgaaagcgcg aaccagagca tcgccaggaa gaaggtcacg
    11701 ccgccggcaa tcatgaagta cagcgtatag tccgcgcgat aggtcccgct cttcggatcg
    11761 tacaccgagc acaggatgcg cacacgatcg atcagatccg tgatgccgac gccaccatcg
    11821 acgatccgcg cgccggtgag cagttgtttc aacggctcgc cgagtcgctc ggcggtgaag
    11881 ctgtcgccgt agacctgtcg gtagacccgt ccctgcgcat ccagcatcgt cacctgcagg
    11941 atgtgatcga aaccaccacg tgtcgccgcg aaggtgaagc cgaagtcccg ggccagcgca
    12001 gcgacatcgg ccgcgtccgg gctcaggaat tcccagttcg gatccgaaat gcgccgctgc
    12061 cgcgcgaatt cctgcatcgc ggccggggtg tcctccggct ggttgaagcc gatggtgacc
    12121 acgttgaact gtcggtcgcc gaagcgcctg tgcatggcgc gtacggcctg gtgcagcacc
    12181 tgcgtgctgg ccggacagat gtggtagcag ccggtgtaga tgaagctcac cagcaacggg
    12241 cggccccgga agctcgacaa ctggacaggc cgcccttgcc gatcgcgcag cgtgtagtcg
    12301 tccggcatgc ggcccacggc cgcctggctg gcacgcagcg ccgcctcacc gtcgagtccc
    12361 ggcgtcgtga tggcgccggt cggcaggtcg gcgacgagcg ggttcgaccc gctcgcctca
    12421 tggacagccc acagcggcgt ccagccgccg ctgccgagcg cagccagcag cagcacgggc
    12481 agcggcgggc gccaccggac gggcatgtgc aggtgcttca tcgatccttg ccgcagcgca
    12541 atcacccgcg tctggctgcg agcggccggc acgaacggcg tgccgtcagc tcagccccca
    12601 gagtgtcgag agatacttcc agttgatgaa gtagtagagc acgaaggcga tcaggaagac
    12661 gaccgcgagc acgaaagtgc ctggcgcgac gaagcccatc gaaccgtagg tctgtgcggc
    12721 ggccgcgggc gccgtgggcg gaatcggggt caccttatcg ctcttggcac cgccctcgag
    12781 ccgcttgccc cacaggagcg agccgaccgt gacgtagatg tagatcgcac caccgacgat
    12841 tgccgcgata ccgctgatgc cgaccaggcc catcatcatg taggcagcgc caggccactc
    12901 gtacgccaac gcggagccgt cgaaggccat gtcccagtgt ctgcgggaga cgcccagcgt
    12961 gccggcaccc atcatgacga ggcagaagaa gtacatcgag aggccgaaca ggtagggctg
    13021 ccacttcgca agtcgcggcg cgatcatctc gcgcttgaac agcaccggaa tcaggtagta
    13081 ggtcagcgcc atgaacgaca gggtcgtacc cacgacgacc gtggcatgga agtgcccggg
    13141 cacgtagatc gtgttgtgga tgatcatgtt gagctgctcc gtgcccatca tgacgcccga
    13201 gatgccgccc aggaagccga agccgatgat cgagatgaac acgccggaga acgtcgggtt
    13261 gccccagggc gccttgc
//
LOCUS       DAOQVY010000062        12919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_5445_length_12919_cov_4.685945, whole genome shotgun sequence.
ACCESSION   DAOQVY010000062 DAOQVY010000000
VERSION     DAOQVY010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12919
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5445_length_12919_cov_4.685945"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..494
                     /locus_tag="PKN91_07250"
     CDS             228..494
                     /locus_tag="PKN91_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27903.1"
                     /translation="MSEKPESDEFDDEEEEEESAAVEDLDLDRVIKDLDLAKRRGQKL
                     GDPAWRRLERLLEDKRTADLVSDFEDYDIGLEDGAKPAPRKPAR"
     gene            complement(495..2525)
                     /gene="paaZ"
                     /locus_tag="PKN91_07255"
     CDS             complement(495..2525)
                     /gene="paaZ"
                     /locus_tag="PKN91_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HNS27904.1"
                     /translation="MRLQSHVAGRWQSGEGAGRALRDATTGQVVAEVASGGIDAAAAL
                     AYARETGNPALRRLTFHERAALLKRLAKYLTERKEELYALSFATGATRADSWIDIEGG
                     IGTLFAYASRGLRELPNSQAFVDGDVERLSKGGSFVGQHVCTPLAGAAVHINAFNFPV
                     WGMLEKLGPALLAGMPAIVKPATATAYLAERAYRLMIESGLLPDGAIQLICGGVGDLF
                     DHLGGQDVVSFTGSASTAGQLRGHPVVLANAVRFIGETDSLNCCVLGQDAAPGTPEFD
                     AYVREVVREMTTKAGQKCTAIRKALVPASATEAVIEALRAALHKVVIGDPRREDVRMG
                     PLAGAGQREEVLARIAELRREAEIVSEAMPDIVGADREQGAFVAPTLLLCRRPAEARA
                     AHTVEAFGPVCTVMPYASITEAVAVARRGEGSLVSSVFTADDAIARELVLGLAPFHGR
                     LLVVNRDCARESTGHGSPMPGLVHGGPGRAGGGEELGGLRGVLHYMQRTALQGSPQTL
                     TAITGRWLRGAHEHVGSVHPFRRTWHELAIGETLRSAERKVTVEDIERFAALTGDTFY
                     AHMDEEAAARNPLFGGRVAHGYFLISAAAGLFVDPPFGPVLANYGLDRLRFAKPVRPG
                     DRIKVRLTCKAKSLRADKGYGEVAWDTEITNQDGEVVASYDVLTMVSADPPG"
     gene            complement(2528..2944)
                     /gene="paaI"
                     /locus_tag="PKN91_07260"
     CDS             complement(2528..2944)
                     /gene="paaI"
                     /locus_tag="PKN91_07260"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HNS27905.1"
                     /translation="MTCTTDSLAEDMRALFGRDRASQAFGMRLLEVAPGRATLAMNVR
                     ADMVNGHDICHGGCIFALADSAFAFACNAGRPVTVAAGAAIDYLRPARLGEELVAVAL
                     ERSRSRRTGLYDVTVQNAAGELVAMFRGRSHQLDGR"
     gene            complement(2950..3738)
                     /gene="paaG"
                     /locus_tag="PKN91_07265"
     CDS             complement(2950..3738)
                     /gene="paaG"
                     /locus_tag="PKN91_07265"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HNS27906.1"
                     /translation="MGYETIEFSVAQGIARLTLNRPDKLNSFNTTMHGEVRAALSRVM
                     PEGARVLVLTGAGRAFCAGQDLGDRAVAPGGQAADLGESIEKNYKPLVLALRRLPLPV
                     IAAVNGVAAGAGANIALACDIVVATRSASFIQSFSKLGLVPDSGGTWTLPRLVGNARA
                     LGLALLGDRLSASDAERFGMIWRCVDDAAFTDSVEALAAQLAAAPTRGLARTKEAIYA
                     SWGRSLDEQLDAERDLQRELGRTSDYAEGVAAFAAKRAPRFTGT"
     gene            complement(3748..4866)
                     /locus_tag="PKN91_07270"
     CDS             complement(3748..4866)
                     /locus_tag="PKN91_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNS27907.1"
                     /translation="MLKFHPLTLMERADCAEDAVSLSFAVPPQLRDVYRFEAGQHIAV
                     RMGDGERRTYSIVSPQGDATLRIGVRLQPDGRVSRFLTHRLAVGESLDVLTPNGSFHT
                     RITPERAGRFIAFAAGSGITSVLSIASTVLAEEPLSRFMLFYGNRTAASTMFLEEVMA
                     LKNRWPDRFAAHFLMSREPQDIALLNGRIDGARVREFARVFFDPAAVDEFFICGPGSM
                     NDDVAAALRELGATGRIHTEYFRVAEVPAAPAAPAAAPASVSAAAPAPTPELAAAGVA
                     AGVGTRVTVLMDGRRRSFAMAAHDTRTVLDAAAEAGIELPFSCRAGVCSTCRARVLKG
                     EVVMEQNHALEDWELAEGFVLCCQAHPTTTELELTYDE"
     gene            complement(4871..5338)
                     /gene="paaJ"
                     /locus_tag="PKN91_07275"
     CDS             complement(4871..5338)
                     /gene="paaJ"
                     /locus_tag="PKN91_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HNS27908.1"
                     /translation="MSRAQPALEADVRRRLAAVADPEIPVLSVVDLGIVRHVRVDADG
                     AVEVGLSPTYSGCPATSVIKANVETALRSGGYREVRIVDVLAPPWTTDWISNEGRRKL
                     HDYGIAPPTAGPIACPQCGSLEITKLSEFGSTPCKSLYRCESCREPFDHFKCI"
     gene            complement(5335..6102)
                     /gene="paaC"
                     /locus_tag="PKN91_07280"
     CDS             complement(5335..6102)
                     /gene="paaC"
                     /locus_tag="PKN91_07280"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HNS27909.1"
                     /translation="MSAGSHGDAHFRYVLRLADTNLVLAQRLGALIGNAPALEEDLGL
                     GNVALDLLGQARMLLTHAGALEGRGRDEDALAFLREERDFLNATLVEQPNGDFAQTIV
                     RQFLFDAWQLATWSRLESSTDPQLAAIAAKGVKEARYHLRFSSGWVVRLGDGTAESHA
                     RAQAALDALWPHTAELFDADEIDRAMQAAGVGVDLAAVCVDWDRQVDGTLAEATLARP
                     ADHPWRWYGKRGQHGEHLGRLLAEMQYMQRTYPGANW"
     gene            complement(6099..6389)
                     /gene="paaB"
                     /locus_tag="PKN91_07285"
     CDS             complement(6099..6389)
                     /gene="paaB"
                     /locus_tag="PKN91_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HNS27910.1"
                     /translation="MSNSREWPLFEVFIRARGGLDHKHVGSVHAADARMAMEHARDLY
                     TRRQEGVSIWVVRSSDIIASDPADADAFFEPARDKVYRHPTFYAIPDEVKTL"
     gene            complement(6386..7297)
                     /gene="paaA"
                     /locus_tag="PKN91_07290"
     CDS             complement(6386..7297)
                     /gene="paaA"
                     /locus_tag="PKN91_07290"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HNS27911.1"
                     /translation="MSMQIEALEERFQARIDADERIEPKDWMPDSYRRTLIRQMSQHA
                     HSEIVGMLPEGNWITRAPTLKRKAILMAKVQDEGGHGAYLYGATETLGVTREQLIEEL
                     LSGAAKYSSIFNYPTPTWADIGAIGWLVDGAAIMNQIPLCRCSYGPYARAMVRICKEE
                     SFHQRQGYDIMLQLARGLDAQRRMAQEALDRWWWPALMMFGPPDADSKHSAQSMRWKI
                     KRFSNDELRARFIEFTVPQVRYLGLTVPQGEIDWSEFRDVLAGHGPCNRERIEARRRA
                     HEQGRWVREAATAYAAKQRDRELKEAS"
     gene            complement(7294..8544)
                     /locus_tag="PKN91_07295"
     CDS             complement(7294..8544)
                     /locus_tag="PKN91_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNS27912.1"
                     /translation="MGAAIEVRVPAEQSEGTRSQVQRWLKAPGDAVREQDPLLELETD
                     KVTVEIASPASGTLLQIVTPEGTEVAPGELLATIATGPATASVTVADAGPGAAAGRDS
                     ATGAAAAAGRADAMSHDATASAAASSRSTALSPAVRRLLAERGLLAADVTGSGAGGRI
                     TVDDVLAMSPRAPAAEVPPDAAQIPARLQPHSAMRRRIAAHMVESLRIAPHVTTVFEA
                     DMSAVLAHRARHRAEFEARGVPLTLTAYFLAACVEAIRAVPEANSRWHEQGLELHDAV
                     HIGVATALEDRGLVVPVVRDVQALDLFGIARELGAVVARARDDRLSPADVRGGTFTIS
                     NHGVSGSLVATPIVINQPQSAILGVGKLEKRPVVVAEDGADRVVVRPRCYVTLTIDHR
                     VMDGHRANRFLEVLVRQLEQVSNR"
     gene            complement(8544..10640)
                     /locus_tag="PKN91_07300"
     CDS             complement(8544..10640)
                     /locus_tag="PKN91_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNS27913.1"
                     /translation="MSAVAQHSSQTGAPQVDWARAAQTVLISRALDDLEESQLHPARR
                     VLYQFSARGHDVTQALLGQMLTGVHDGVALYYRSRPLMLALGLPPETALASTMMRAGS
                     VSEGRDIGVVFNLPRRHGPCVLPACGGVGAQYTPAVGWAQAIRYHSQVLRDRAWTGSV
                     AVVHGGEASTASNGFWSALNIATTQRLPLLFYIEDNGYGISVPSTLQTPGANIAANLA
                     SFRNLKVFDGDGCDPCNAALLMAQAIAGVRAGEGPALLRLTMPRLSGHSGQDTQTYKS
                     AEELAAERLRDPLRQLRARIVPSLLTESGWAAIEARAAATIEEALAQVEARGTPDPAT
                     ASRYVFSEQRPDGTPDAQQQGGLRGDGIEPPAGTELARAEGPRINMVTAIRRTLEHEL
                     ATNPRVVVFGEDVGRKGGVHAATLGLQEKFGAARVFDTSLSEEGIIGRAVGLALAGLM
                     PVPEIQFRKYAEPATEQINDCGTMRWRTANRFAAPMVVRMPGGHFKCGDPWHSQSNEV
                     QFAHAIGWRVAMPSNAEDAVGLLRAALRGNDPTIFFEHRAMLDAAWARRPWPGDDFVV
                     PFGRARIVRPGRELSVVTWGAMVERCEAAADESAVDVEILDLRTIVPWDREAVLESVR
                     RTRRCLIVHEDTLTAGFGAEIAATLAREAFFDLDAPIERIAMPDVPSPHSPVLLDSVL
                     PGVPCIVRAMRELASL"
     gene            10757..11242
                     /locus_tag="PKN91_07305"
     CDS             10757..11242
                     /locus_tag="PKN91_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNS27914.1"
                     /translation="MAFFTMSLDRLDARIVAELQADGRLSVVELGERIGLTATPCARR
                     VRQLEQRGVIVGYTAIVDPARLGLNVQAFIQVKLDRHTDENVELFERELAAIEQIVSC
                     HATTGEYDFMLHVTAPDLDGLSTLVLKQLLRIPGVRDVYSSIVLDTVKRPAHLPVGHL
                     G"
     gene            complement(11239..12342)
                     /locus_tag="PKN91_07310"
     CDS             complement(11239..12342)
                     /locus_tag="PKN91_07310"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HNS27915.1"
                     /translation="MRAALPGLFAVAMAAAMSATVAGSPVPAASALVQPAPVVPGLVM
                     AFPRDHGSHPAFRTEWWYATGWLRTDSGEDLGFQITFFRSLQPAAATNPSAFAPRQIL
                     IAHAAISDAGRGRLWKDERVARAGFGLAEAREGDAAVWIDDWRLERVAETFAVHAPAR
                     DFGLELALRERQPPLLNGEAGFSRKGAAPLAASYYYSLPQLEVRGHVVREGRRVAVTG
                     TAWLDHEWSSEVLEPGAVGWDWFSVNLADGGALMAFRIRDRDGRQRWAGGTLRAADGS
                     SRHYAPEEIEFIPGREWRSPRTAISYPVQWSVRVGERRLTLEPLMDDQESDTRLTTGA
                     IYWEGAVRVREDARLLGLGYLELTGYGGALELR"
     gene            complement(12339..>12919)
                     /locus_tag="PKN91_07315"
     CDS             complement(12339..>12919)
                     /locus_tag="PKN91_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS27916.1"
                     /translation="MAATGDRTVNEGSLWLASGADAPSVARNLQAALALGDSLQLRTT
                     TEVRERSLANFDRAFLVTYALEAIAVLIGLLGVGVTAAATALARRAEFGMLRHVGLLR
                     RELAAMLAGEGLLMSAIGVAFALVLGGALSLVLVHVVNRQSFLWSIDFAIPWLQLALL
                     ALALVFAAAVTTVVSGRAALGGDALRAVREDW"
BASE COUNT         2058 a         4630 c         4308 g         1923 t
ORIGIN      
        1 cgcgaactcg acggcctgct ggccgcactg gtcggcctgc tggggccgct gctcatcatc
       61 gtgatgggcc tgttcgtgat gggcatcgta tttgcaatgc tgctgccgat cttcgagatg
      121 aacacgctga ttcgctgacc tgccaatcgg acattgcgcc cgggcaattt tcgcgctaca
      181 aattttgccc gacagaccca accaccacca cggttccctt tcgccacatg agcgagaagc
      241 ccgaatccga cgagttcgac gacgaggaag aggaagagga aagtgccgca gtcgaggacc
      301 tcgacctcga tcgggtcatc aaggacctcg acctcgccaa gcgccgcggc cagaagctcg
      361 gcgacccggc ctggcgtcgc ctcgagcggc tgctcgagga caaacgcacc gctgatctgg
      421 tcagcgactt cgaggactac gacatcggcc tcgaggatgg agcgaagccg gcgccgagga
      481 aaccggcgcg ctgactatcc gggcgggtcc gcgctgacca tcgtcagcac gtcgtagctc
      541 gcgaccacct cgccgtcctg gttcgtgatc tcggtgtccc aggccacctc gccgtagcct
      601 ttgtcggcgc gcagcgactt cgccttgcag gtgaggcgca ccttgatgcg gtctcccggt
      661 cgcaccggct ttgcgaagcg cagccggtcg aggccgtagt tcgcgagcac cggaccgaag
      721 ggcgggtcga cgaacaggcc ggccgccgcc gagatgagga agtagccgtg ggccacccgt
      781 ccaccgaaca gcggattacg ggccgcggcc tcctcgtcca tgtgcgcata gaacgtgtcg
      841 ccggtcagcg ccgcgaagcg ctcgatgtcc tcgaccgtca ccttgcgctc ggccgagcgc
      901 agcgtttcgc cgatggcgag ctcatgccat gtccggcgga agggatgcac gctgccgacg
      961 tgctcgtgcg ccccacgcag ccagcgtcct gtgatcgcgg tcagcgtctg cgggctgccc
     1021 tgcagcgcgg tgcgctgcat gtaatgcagc acgccgcgca gtccgcccag ttcctcgccg
     1081 ccgccggcgc gccccggccc gccatggacg aggccaggca tgggcgaacc gtgtccggtc
     1141 gactcccgcg cgcaatcgcg attcaccacc agcaggcggc cgtggaaagg cgcgaggccg
     1201 agcaccagtt cgcgtgcgat tgcgtcgtcc gcggtgaaca ctgacgacac caggctcccc
     1261 tcgccccgtc gcgcaacggc cacggcctcg gtgatcgacg cgtacggcat aaccgtgcac
     1321 accgggccga atgcctccac cgtgtgggcg gcacgcgcct cggcgggcct gcggcacagg
     1381 agcagcgtcg gggcgacgaa ggcgccctgc tcccgatccg ccccgacgat gtcgggcatc
     1441 gcctcgctga cgatctctgc ttcgcgccgc agctccgcga tgcgcgcgag cacctcctcg
     1501 cgctggccgg cgccggccag cgggcccatc cggacatctt cgcgccgcgg atcgccgatg
     1561 acgaccttgt gcagcgcggc gcgcaacgcc tcgatgaccg cctcggtcgc cgacgcgggc
     1621 accagggcct tgcgtatcgc cgtgcacttc tggccggcct tcgtggtcat ctcgcgcacc
     1681 acttcgcgca cgtaggcgtc gaactcgggc gtgcccggcg cggcgtcctg gccgagcacg
     1741 cagcagttca gcgaatcggt ctcgccgatg aagcgcaccg cgttcgcgag cacgaccggg
     1801 tggccgcgca gctggccggc ggtcgacgcc gatcccgtga acgacacgac atcctggccg
     1861 cccaggtgat cgaacaggtc gccgacaccg ccacagatga gctgtatcgc accgtcgggc
     1921 aacaggcccg attcgatcat cagccggtag gcacgttccg cgagatacgc ggtcgcggtc
     1981 gccggcttga cgatcgccgg catgccggcg agcagcgcag gcccaagctt ctcgagcatg
     2041 ccccacaccg ggaaattgaa ggcattgatg tgcacggcgg cgcccgcgag cggcgtgcag
     2101 acgtgctggc cgacgaagct gccgcccttc gagagccgtt cgacgtcgcc atcgacgaac
     2161 gcctggctat ttggcagttc gcgcagccca cggctcgcgt atgcgaacag cgtgccgata
     2221 ccgccttcga tgtcgatcca ggagtcggcg cgcgtcgcgc cggtcgcgaa cgacagcgcg
     2281 tacagctcct ccttgcgctc ggtgagatac ttcgcgagcc gcttcagcaa cgcggcgcgc
     2341 tcatgaaagg tcaggcgtcg cagcgccggg ttgccggtct cgcgcgcgta cgcgagcgcc
     2401 gcggcggcat cgatgccgcc cgaagccacc tcggcgacca cctgcccggt ggtcgcgtca
     2461 cgcagcgctc ggcctgcgcc ctcacccgac tgccagcgtc ccgcgacatg gctctgcaac
     2521 cgcatgttca tctcccgtcc agctggtgcg accggccgcg aaacatcgcg accagctcac
     2581 cggccgcgtt ctggacagtc acgtcataaa ggcccgtacg gcgcgagcgc gagcgctcga
     2641 gcgccacggc gacgagttcc tcgcccaggc gcgccggacg caggtagtcg atcgccgcgc
     2701 ccgcggcgac cgtcaccggc cggcccgcgt tgcaggcaaa ggcgaaggcg ctgtcggcga
     2761 gggcgaagat gcacccgcca tggcagatgt cgtgaccgtt caccatgtcc gcgcgtacgt
     2821 tcatcgcgag tgtcgcgcga ccgggcgcca cctcgagcag gcgcatgccg aaggcctgag
     2881 atgcccggtc acgcccgaag agggcgcgca tgtcctccgc cagcgaatcc gtggtgcagg
     2941 tcacggcgtt caggtcccgg tgaaccgggg agcacgcttg gcggcgaatg ccgcgacccc
     3001 ttccgcatag tccgacgtgc ggccgagctc gcgctgcagg tcgcgttcgg cgtcgagctg
     3061 ctcgtcgagc gagcgccccc acgacgcgta gatggcctcc ttggtgcgcg caaggccgcg
     3121 cgtgggagct gcggcgagct gcgccgcgag cgcctcaacg ctgtccgtga acgccgcatc
     3181 gtcgacgcag cgccagatca tcccgaagcg ctcggcatcg cttgcggaca gtctgtcgcc
     3241 gagcagcgcc aggccgaggg cacgggcatt gccgacgagg cgtggcagag tccaggtgcc
     3301 acccgaatcc ggcaccagcc cgagcttgct gaacgactgg atgaagctcg ccgagcgtgt
     3361 cgccaccacg atgtcgcagg cgagcgcgat attggccccc gccccggccg cgacgccgtt
     3421 caccgccgcg atgaccggca acggcagccg gcgcagcgcg agcaccagcg gcttgtagtt
     3481 cttctcgatc gactcaccga gatcggccgc ctgaccgccc ggcgcgaccg cgcggtcgcc
     3541 gaggtcctgc ccggcgcaga acgcgcgccc cgcgccggtc agcaccagca cgcgcgcgcc
     3601 ctctggcatc acgcgcgaca gggccgcccg cacctctccg tgcatggtgg tgttgaagct
     3661 gttgagcttg tcgggccgat tcaacgtgag gcgcgcgatg ccctgcgcga cgctgaattc
     3721 gatcgtctcg taccccatgt gcgtgtccta ttcgtcgtag gtgagctcga gttcggttgt
     3781 ggtcgggtgc gcctgacagc agagcacgaa gccttcggcc agttcccagt cctcgagcgc
     3841 gtggttctgc tccatgacga cctcgccttt cagcacgcgg gcacggcagg tcgaacaaac
     3901 cccggcgcgg caggagaacg gcagctcgat gccggcctcg gccgcagcat cgagtacggt
     3961 gcgggtgtcg tgcgccgcca tcgcgaagct gcggcgccgc ccgtccatga gcacggtgac
     4021 gcgtgtgccg actccggccg ccacgcccgc ggccgccagt tcgggcgtgg gtgcgggcgc
     4081 ggctgccgac acggaggccg gggcggcggc cggcgcggcc ggcgccgccg gcacctcggc
     4141 caccctgaag tactcggtgt ggatccggcc tgtggcgccg agctcgcgca gcgcggcagc
     4201 cacatcgtcg ttcatcgaac cgggcccgca gatgaagaac tcatcgaccg ccgcgggatc
     4261 gaagaagacc cgggcgaact cgcgcacacg cgcaccatcg atgcgtccgt tcagcagtgc
     4321 gatgtcctgc ggctcgcggc tcatcaggaa atgcgccgcg aagcggtccg gccagcgatt
     4381 cttcagcgcc atcacctctt cgaggaacat ggtgctcgcc gccgtgcggt tgccgtagaa
     4441 cagcatgaag cggctgagcg gctcctccgc gagcacggtc gatgcgatcg acagcaccga
     4501 cgtgatgccg ctgcccgccg cgaaggcaat gaatcggccg gcccgctccg gcgtgatgcg
     4561 ggtgtggaag ctgccgttcg gcgtgagcac atcgaggctc tcgcccaccg caagccggtg
     4621 cgtgaggaac cgggaaaccc gcccgtcagg ctgcaggcgc acgccgatgc gcagcgtggc
     4681 gtcgccctgc gggctcacga tcgagtaggt gcgccgctcc ccatcgccca tgcgcaccgc
     4741 gatgtgctga ccggcctcga agcggtacac gtcgcgcaac tgcggcggca ccgcgaagct
     4801 gaggctcacg gcatcttcgg cgcagtcggc gcgctccatg agcgtcagcg gatggaactt
     4861 gagcacgatt tcagatgcac ttgaagtggt cgaacggctc gcggcagctc tcgcagcgat
     4921 acagcgactt gcagggcgtc gagccgaact cactgagttt cgtgatctcg agcgagccgc
     4981 attgcggaca ggcaatgggc cccgctgtcg gcggcgcgat gccgtagtcg tgcagcttgc
     5041 gtcgaccctc gttcgagatc cagtcggtcg tccacggcgg cgccagcacg tcgacgatgc
     5101 gcacctcgcg atagccgccg ctgcgcagcg cagtctcgac attcgccttg atgacgctcg
     5161 tggccgggca accggaatac gtgggggaca ggcccacctc gacggcaccg tccgcatcga
     5221 ctcgcacatg ccgcacgatg ccgaggtcga ccacgctcaa caccggaatc tccggatcgg
     5281 cgactgccgc gagccgccgc cgcacatccg cctcgagcgc cggttgcgcg cggctcacca
     5341 gttcgctccg ggataggtgc gctgcatgta ctgcatctcg gcgagcaacc gtccgaggtg
     5401 ctcgccatgc tgcccgcgct tgccgtacca gcgccacgga tgatcggccg gccgcgccag
     5461 ggtcgcctcg gccagggtcc cgtcgacctg gcggtcccag tccacgcaca cggcggcgag
     5521 atcgacgcct acccccgccg cctgcattgc gcggtcgatt tcgtcggcat cgaagagctc
     5581 cgcggtatgc ggccacaggg catcgagggc ggcctgcgca cgggcgtggc tctccgcggt
     5641 gccgtcgccg aggcgcacga cccagcccga gctgaaacgc aggtgatagc gcgcctcctt
     5701 gacccccttg gccgcgatcg ccgcgagctg cgggtcggtc gaggactcga ggcgcgacca
     5761 ggttgcgagc tgccacgcat cgaacaggaa ctggcgcacg atcgtctggg cgaaatcgcc
     5821 attcggctgc tcgacgagcg tcgcgttcag gaaatcgcgc tcctcgcgca ggaaggcgag
     5881 cgcgtcctcg tcccgacccc ggccttcgag cgcgccggca tgggtgagca gcatgcgcgc
     5941 ctgacccagc aggtcgagcg caacgttgcc gagcccgagg tcttcctcca gcgccggcgc
     6001 gttcccgatc agcgcgccga ggcgttgcgc gagcaccagg ttggtatcgg cgaggcgcag
     6061 cacataacgg aagtgcgcat ccccgtgaga cccggcgctc acagcgtctt cacctcgtcc
     6121 gggatcgcgt agaaggtcgg atggcgatac accttgtcgc gcgccggttc gaagaaggca
     6181 tcggcatcgg ccggatcgga cgcgatgatg tcgctcgagc gcaccaccca gatgctgacg
     6241 ccctcctggc ggcgcgtgta caggtcgcgt gcatgctcca tcgccatgcg cgcatcggcc
     6301 gcgtgcacac tgcccacgtg cttgtgatcg agtccgccgc gggcgcgaat gaagacctcg
     6361 aacaacggcc attcccgcga attgctcatg acgcctcctt cagttcgcgg tcgcgctgct
     6421 tggctgcgta tgccgtggcc gcctcgcgca cccagcgtcc ctgctcgtgg gcacgccggc
     6481 gtgcctcgat gcgctcacgg ttgcagggac cgtggccggc gagcacatcg cggaactcgc
     6541 tccagtcgat ctcgccctgc gggaccgtga gaccgaggta acgcacctgc ggcaccgtaa
     6601 actcgatgaa gcgcgcgcgc agctcgtcgt tgctgaaacg cttgatcttc cagcgcatcg
     6661 actgtgccga atgcttcgag tcggcatcgg gcggcccgaa catcatcagc gcgggccacc
     6721 accagcgatc gagtgcctcc tgcgccatgc gtcgctgcgc atcaaggccg cgcgcgagct
     6781 gcagcatgat gtcgtagccc tggcgctggt ggaaactctc ctccttgcag atgcgcacca
     6841 tcgcacgcgc atacgggccg taggaacagc ggcacagcgg gatctggttc atgatcgccg
     6901 cgccatcgac cagccagccg atcgcgccga tgtcggccca ggtcggggtg gggtaattga
     6961 agatgctcga atacttcgcg gcgcccgaca gcagctcctc gatcagctgc tcgcgagtga
     7021 cgccgagtgt ttcggtggcc ccgtatagat aagcgccgtg gccgccctcg tcctgcacct
     7081 tggccatcag gatcgccttg cgcttcaggg tcggcgcacg cgtgatccag ttgccctcgg
     7141 gcagcatgcc gacgatctcg gagtgcgcgt gctgcgacat ctgccggatc agtgtgcggc
     7201 ggtaggaatc aggcatccag tcctttggct cgatgcgctc atcggcatcg atccgcgcct
     7261 ggaaccgctc ctcgagggct tcgatctgca tgctcatcga tttgacacct gttcgagctg
     7321 acgcaccagc acttcgagga agcggttggc gcgatggccg tccatcaccc gatgatcgat
     7381 agtgagcgtg acgtagcagc gcggtcgcac gacgacacgg tccgcgccgt cttccgcgac
     7441 gaccaccggc cgcttctcga gcttgccgac acccagtatc gccgactgcg gctggttgat
     7501 gacgatcggc gtcgcgacga ggctgccgct gacgccgtgg ttggagatcg tgaacgttcc
     7561 gccacgcacg tccgccggcg agagccggtc atcgcgcgca cgcgccacca ctgcgccaag
     7621 ctcgcgcgcg ataccgaaga gatcgagcgc ctgcacgtca cgcaccaccg gcaccacgag
     7681 gccgcgatcc tcgagcgccg tcgcaacacc gatatgtacg gcatcgtgca gctcgaggcc
     7741 ctgttcgtgc cagcgactgt tggcctctgg caccgcccgg atcgcctcca cgcaggccgc
     7801 aaggaagtac gcggtcagtg tcagcggcac gccacgcgct tcgaattcgg cccggtgccg
     7861 cgcgcgatgc gcaagtaccg cggacatgtc ggcctcgaac accgtcgtga catgcggcgc
     7921 gatgcgcagg ctctcgacca tgtgtgccgc gatgcgccga cgcatggcac tgtggggctg
     7981 caggcgcgcc ggaatctgcg ccgcgtccgg cggcacctcg gccgccggcg cacgcggaga
     8041 catggccagc acatcgtcga ccgtgattcg tccgcccgcc ccgctacccg tcacatcggc
     8101 cgcgagcaga ccgcgctcgg ccagcagccg gcgcacggcg gggctcaatg cggttgaacg
     8161 actcgacgcc gcagctgacg cagtggcgtc gtgcgacatg gcgtccgccc ggccggcggc
     8221 agccgcagcg cccgttgcac tgtctcggcc ggccgccgcg cccggcccgg cgtcagccac
     8281 cgtgacgctc gcggtcgcgg gcccggtggc gatcgttgcg agcagctcgc ccggcgccac
     8341 ctccgtgccc tcaggtgtca cgatctgcag cagcgtgccg ctcgccggcg aggcgatctc
     8401 gaccgtgacc ttgtcggttt cgagttcgag caagggatcc tgctcgcgca ccgcatcgcc
     8461 cggcgccttc agccagcgct ggacctgcga gcgcgtaccc tccgattgct ccgccgggac
     8521 gcgaacctcg atcgccgcgc ccatcacagg cttgccagct cgcgcatcgc gcgaacgatg
     8581 cacggcacgc ccggcaggac gctatcgagc aacaccgggc tgtgcgggct cggcacgtcc
     8641 ggcatggcga tgcgctcgat cggcgcatcg aggtcgaaga aagcctcgcg cgccagcgtc
     8701 gcggcgatct ccgcgccgaa tccggcggtc agcgtgtcct cgtgcacgat caggcagcgg
     8761 cgggtacgcc gcaccgattc cagcacagcc tcgcggtccc agggcacgat cgtgcgcagg
     8821 tcgaggatct cgacatcgac cgcggattcg tccgccgccg cttcgcagcg ctcgaccatc
     8881 gcaccccagg tgacgaccga gagctcacgg ccagggcgca cgatgcgcgc gcgtccaaag
     8941 ggcacgacga aatcgtcccc cggccacggg cggcgcgccc aggcggcatc gagcatcgcg
     9001 cgatgctcga agaagatcgt cgggtcgttg ccgcgcaatg ccgcgcgcag caggccgacg
     9061 gcatcctcgg cattcgatgg catcgccacg cgccagccga tcgcgtgcgc gaactgcact
     9121 tcgttgctct gactgtgcca cgggtcgccg cacttgaagt ggccacccgg catgcgcacg
     9181 accatcggcg ccgcgaaccg gttcgcggtg cgccagcgca tcgtgccgca atcattgatt
     9241 tgttcggtcg ccggctcggc gtacttgcgg aactggatct ccggcaccgg catgaggccc
     9301 gcgagcgcga ggcccacggc gcgaccgatg atgccctcct ccgaaagcga ggtatcgaac
     9361 acccgcgcag cgccgaactt ctcctgcaac ccgagcgtgg cggcatgcac gccgcctttg
     9421 cgacccacgt cctcgccgaa caccacgacc cgcgggttgg tggcgagttc gtgctcgagc
     9481 gtgcggcgaa tcgccgtcac catattgatc cgcggccctt cggcgcgggc cagctccgtg
     9541 ccggcaggcg gctcgatgcc atcgccgcgc agtccaccct gctgctgcgc atccggcgtg
     9601 ccgtcgggac gctgctcgct gaagacatac cgcgacgccg tggcggggtc cggcgtgccg
     9661 cgagcctcga cctgcgcgag cgcctcctcg atcgtggcag cggcccgagc ctcgatcgcg
     9721 gcccacccgg actcggtgag cagcgacggg acgatgcgcg cacggagctg ccgcagcgga
     9781 tcgcgcagcc gctcggccgc gagctcttcg gcactcttgt acgtctgcgt gtcctgcccc
     9841 gagtgtcccg acaggcgcgg catggtgagg cgcagcagcg ccggcccttc gcccgcacgc
     9901 acgccggcga ttgcctgcgc catcagcagc gcggcgttgc agggatcgca gccatcgccg
     9961 tcgaagactt tcagattgcg aaacgacgcc aggttcgccg cgatgttggc accgggggtc
    10021 tgcagggtcg agggcaccga gatgccgtag ccattgtcct cgatatagaa aagcagcggc
    10081 agtcgctgcg tcgtcgcgat attgagcgcc gaccagaagc cattgctcgc ggtcgatgcc
    10141 tcaccgccat gcaccacggc aacgctgccc gtccaggcgc gatcgcgcag cacctgggag
    10201 tgatagcgga tcgcctgcgc ccagccgacg gccggcgtgt actgcgcgcc gacgccgccg
    10261 caggccggca gcacgcaggg accgtgccga cgcggcaggt tgaaaacgac gccgatgtcg
    10321 cgcccttcgc tgacgctgcc cgcgcgcatc atcgtcgagg cgagcgcggt ctcgggcggg
    10381 aggccgagtg cgagcatcag cggtcgcgag cgataataga gcgcgacacc atcgtgcacc
    10441 ccggtgagca tctgcccgag cagggcctgc gtgacatcgt ggccgcgggc ggaaaactgg
    10501 tagagcaccc gtcgcgcggg atgcagctgc gactcctcga ggtcgtcgag cgcacgcgag
    10561 atgagtaccg tctgtgcggc gcgcgcccaa tcgacctggg gcgctcccgt ctgcgaggaa
    10621 tgctgtgcga ccgccgacat gcccgtgacc ctccgttggc caacggcagc gtaaaccagc
    10681 accggaagcg aaaaacgcca aattacgctc ataaacggca gcgttttagc ggtatacttt
    10741 tgtttagtac ttgtgaatgg cgtttttcac catgtcgctc gaccgcctgg atgcgaggat
    10801 cgtggcagag ctgcaggccg acggtcggct gagcgtcgtc gaactcggcg agcgtatcgg
    10861 tctgaccgcc acaccctgtg cccggcgcgt gcgccagctg gagcagcgcg gggtcatcgt
    10921 cggctacacc gcgatcgtcg atccggcaag gctcggactc aacgtgcagg ctttcatcca
    10981 ggtgaagctc gatcggcaca cggatgagaa tgtagaactg ttcgagcgcg aactcgccgc
    11041 gatcgagcag atcgtcagtt gccacgcgac gaccggcgag tacgatttca tgctgcacgt
    11101 caccgcgccc gatctcgacg gactgagcac tctcgtgctg aagcagttgc tgcgcattcc
    11161 gggagtgcgg gatgtgtact cgagcatcgt gctcgacacc gtgaagcgac cggcccacct
    11221 gcccgtgggg cacctcggct agcgtagctc gagcgccccg ccgtaaccgg tgagttccag
    11281 gtaacccagg ccgaggaggc gcgcgtcctc gcgcacgcgc accgcgcctt cccagtagat
    11341 cgcccccgtc gtgaggcgcg tgtcgctttc ctggtcgtcc atcagcggct cgagcgtcag
    11401 gcgccgctcg cccacccgca ccgaccactg caccggatag ctgattgcgg tgcgcggcga
    11461 acgccactcg cgccccggga tgaactcgat ctcctcagga gcgtagtgcc ggctcgatcc
    11521 atctgccgcg cgcagcgtgc cgcccgccca gcgctggcgg ccgtcacggt cgcggatgcg
    11581 aaacgccatc agcgcacctc catcggccag gttcacactg aaccagtccc agcccacggc
    11641 gcccggctcg agcacttcgc tcgaccactc gtggtcgagc cacgccgtgc cggtcacggc
    11701 gacccgcctg ccctcgcgca ccacgtggcc gcgcacttcg agctgcggca ggctgtagta
    11761 gtagcttgcg gcgagcggcg cggcgccctt gcggctgaag ccggcctcac cgttcagcag
    11821 gggtggctga cgctcgcgca gcgcgagctc gagaccgaaa tcgcgcgccg gcgcgtgcac
    11881 cgcgaaagtt tctgccacgc gctcgaggcg ccagtcgtcg atccacacgg cggcatcgcc
    11941 ctcgcgcgct tcggcgagtc caaaccccgc gcgcgcgacc cgctcgtcct tccagagccg
    12001 cccgcgcccg gcgtcgctga tcgcagcatg agcgatcagg atctggcgcg gcgcgaatgc
    12061 gctcggattg gtcgcggcgg cgggctgcag cgagcgaaag aacgtgatct ggaagccgag
    12121 gtcctcgccc gagtccgtgc gcagccagcc ggtcgcatac caccactcgg tgcgaaacgc
    12181 ggggtggctg ccgtgatccc gcggaaaggc catcacgagg cctggcacga ctggcgcagg
    12241 ctgcaccagc gctgacgcgg cggggaccgg actgcccgcc actgtggcgg acatcgccgc
    12301 agccatcgcg acggcgaaca gacccggcag cgccgccctc accagtcctc ccgtacggcg
    12361 cgcagcgcat cgccgccgag cgcggcgcgg ccgctgacga ccgtcgtgac cgccgccgcg
    12421 aaaaccagcg cgagcgcgag cagcgcgagc tgcagccacg gaatcgcgaa atctatgctc
    12481 cacaggaacg actggcgatt gacgacgtgc accagcacga gactcagcgc cccgcccagt
    12541 acgagcgcga acgccacgcc gatggcactc atcaggagtc cctcgcccgc cagcatcgcc
    12601 gcgagctcgc ggcgcaggag tccgacatgg cgcagcatgc cgaactccgc gcggcgcgcg
    12661 agtgcggtgg ccgcggcggt gacgccgacg ccgagcaggc cgatcagcac ggcgatcgcc
    12721 tcgagcgcgt aggtcacgag gaaggcgcgg tcgaagttcg cgagcgaacg ctcgcgcacc
    12781 tcggtcgtag tgcgcagctg cagtgaatcg ccgagcgcga gcgccgcttg caggttccgc
    12841 gccaccgacg gcgcgtcggc cccgctcgcg agccacagcg aaccttcgtt cacggtccga
    12901 tcgcccgttg cggccatgt
//
LOCUS       DAOQVY010000063        12839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_5487_length_12839_cov_5.005084, whole genome shotgun sequence.
ACCESSION   DAOQVY010000063 DAOQVY010000000
VERSION     DAOQVY010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12839
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5487_length_12839_cov_5.005084"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PKN91_07320"
     CDS             <1..213
                     /locus_tag="PKN91_07320"
                     /inference="COORDINATES: protein motif:HMM:NF015272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HNS27917.1"
                     /translation="AEKDWDLYEFAECLIDVDYKFQTWRFQHMKTVERIIGYKPGTGG
                     TGGVSYLAKALELKFFPELWQIRTSM"
     gene            complement(170..826)
                     /locus_tag="PKN91_07325"
     CDS             complement(170..826)
                     /locus_tag="PKN91_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HNS27918.1"
                     /translation="MRVEQRYRYVWKPLVFIAALLPALSMLAGVFGIAGQRLGADPVE
                     ELLLTCGKTALNLLLLTLAVTPLARIAHRPQLIRLRRMLGLFAFAYAALHFAVYLALD
                     RAFDASMVVEDITRRPYITIGFTALLLLLPLAATSTQRMMRRLGRRWQHLHRLVYLIA
                     ILGVWHYYWQVKRDVTEPLVYAGILAVLLGWRVWQRRGARVRAAATSTYGSATAPGRT
                     "
     gene            complement(838..1782)
                     /gene="msrP"
                     /locus_tag="PKN91_07330"
     CDS             complement(838..1782)
                     /gene="msrP"
                     /locus_tag="PKN91_07330"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HNS27919.1"
                     /translation="MIFKYHQPPPSEITPPGVYLDRRRWIAAATASLAFAGAGDAAEA
                     PPLAKLAAKRNARYSVAETTTAYEDITSYNNFYEFGTGKGDPQRNAATLRTRPWSVTI
                     AGEAETRGSFTLEDILKPHALEERVYRLRCVERWSMVIPWVGFPLGDLLRRFKPTADA
                     KFVAFRTLHDPRQMPGQSAGWLEWPYVEGLRIDEAMHPLALLAVGLYGRVLPNQNGAP
                     LRLVVPWKYGFKSIKSIVGITFTRTMPPTSWNIAAPREYGFYANVNPAVAHPRWSQAT
                     ERRIGAGLFDATRPTEPFNGYAAEVAGLYRGMDLRRLF"
     gene            complement(1873..2544)
                     /locus_tag="PKN91_07335"
     CDS             complement(1873..2544)
                     /locus_tag="PKN91_07335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HNS27920.1"
                     /translation="MSTVGRVVAVFDLDGTITRRDTLFPYVWRYLARRPWRLARLLAV
                     VPALLAYLFAGRDRGRLKAALLRHTLRGETRAAIEAWNAAYLPRVIARDLLPGAVAAL
                     ARERQRGATLVLMSASVDLYVPALGKLLGFDHVVCTGVRWEDGVLDGALATPNRRGEE
                     KARCLERLRADLAPVASITAYGNGSSDLPHMRLAERGVLVNGPRRARAEAAVLGIECV
                     DWRGT"
     gene            complement(2544..5240)
                     /gene="acnA"
                     /locus_tag="PKN91_07340"
     CDS             complement(2544..5240)
                     /gene="acnA"
                     /locus_tag="PKN91_07340"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HNS27921.1"
                     /translation="MTDSFKARTQLAVGSRTYEIMSLKALPQQKVARLPYSLKILLEN
                     LLRFEDGVNVTRKDIEAVLDWDPQSAPSHEIAFTPARVIMQDFTGVPCVVDLAAMREA
                     ITRLGGDPEMVNPLAPAELVIDHSVQVDAYGTPRSLEENNRIEFERNGERYAFLRWGQ
                     AAFRNFKVVPPNTGIVHQVNLEFLGRVVFDAERDGVRQAYPDTVVGTDSHTTMINGLG
                     VLGWGVGGIEAEAAMLGQPIPMLIPQVIGFRLVGSLRPGATATDLVLTVTEMLRKRGV
                     VDRFVEFFGDGLANLPLADRATLGNMSPEFGSTCAIFPIDTETVRYLRLSGRPEEQIA
                     LVEAYARAQGLWRENGQPDAQYTDVLELDLASVEPSLAGPKRPQDRVPLRSAQAAYRT
                     ALKPMAEERARKSGATGGAQVTLDGRSFELSDGAVLIAAITSCTNTSNPYVMIGAGLL
                     ARNAARRGLTAKPWVKTSLAPGSRVVTDYLRKAELLDDLASAGFYLVGYGCTTCIGNS
                     GPLRPEISAAVAAGDIVATSVLSGNRNFEGRVHPEVKMNFLASPPLVVAYALAGTLDI
                     DLTTDALGNDADGKPVYLKDIWPADHEVADTVQRAIDSGMFRKSYEGVFAGDEHWSSI
                     KVPAGKIYKWDDRSTYVKNPPYFDGMTMQPEAVSDIKGARVLALLGDSVTTDHISPAG
                     NIAKSSPAARYLTEQGVEQKDFNSYGSRRGNHEVMMRGTFANIRLRNLLAPGTEGGVT
                     VHLPSGEQSSIYDAAMRYRTERTPLVVLAGKEYGTGSSRDWAAKGTMLLGVKAVIAES
                     FERIHRSNLIGMGVLPLQFPAGESAASLGLTGREVIDIVGVADETSKTVKVVATPLDG
                     GKPITFEARVRIDTPKERDYYRHGGILQYVLRQLAGASRAAA"
     gene            complement(5273..6403)
                     /gene="mnmA"
                     /locus_tag="PKN91_07345"
     CDS             complement(5273..6403)
                     /gene="mnmA"
                     /locus_tag="PKN91_07345"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNS27922.1"
                     /translation="MSARDTIIVGLSGGVDSAVAALRLLEDGHDVQGLFMTNWDDDDG
                     YCTAAQDFQDARAVAEVLGIALHRVDFRREYRERVFAHFLAEYSAGRTPNPDVACNRE
                     IKFGICLAHARRLGASRYATGHYARLVAGPEGIELHKGTDPARDQSYFLHSIEPEALG
                     SIAFPIGDLYKDEVRERARRAGLPVYDKPDSTGICFIGERPFREFLSQYIVSEPGPIE
                     TADGERIGTHRGLAFYTLGQRGGLEIGGQSGHDEGAWYVARKDVARNALIVVQGHDHP
                     LLQSTALVTEAFHWLARVPGGAFRAMAKVRYRQADQPVDVMPLAGGRVRLNFDQPQRA
                     VAPGQYAVLYQGTRCIGGGVIDSTRPVGAESHAGPAAAIIVG"
     gene            complement(6441..6926)
                     /locus_tag="PKN91_07350"
     CDS             complement(6441..6926)
                     /locus_tag="PKN91_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNS27923.1"
                     /translation="MGSRPAVTVAAVVERDGRFLLVEESIRGQRVLNQPAGHVEDRET
                     LLEAVIRETREESAWRFVPADVLGIYLWRNARTGKSTLRIAFSGEVSDHDPRAPLDRG
                     IVGTHWLTREELEAEHTRLRSPLVLRCIDDYLAGQRLPLASLAHLDLQAAVHMRAVKV
                     G"
     gene            complement(6928..7488)
                     /locus_tag="PKN91_07355"
     CDS             complement(6928..7488)
                     /locus_tag="PKN91_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27924.1"
                     /translation="MSSTGEFELSLLSQTPAKGLELPPLERPPQVNVVTTQEEVLAAT
                     ILVAGSAQRLMSIYTPNLEPDLYDQTPFLDIVKRFVLARSFAKVRVLLGDGSRMIRDV
                     HRFVAMSRRLTSYIDIRVLGEDAPKPHPGYIIADDRAILYRTNAAQWDGIADLDNPAV
                     ARMHLADFDSQWVTHAPDFGYRVARR"
     gene            complement(7623..8999)
                     /gene="purB"
                     /locus_tag="PKN91_07360"
     CDS             complement(7623..8999)
                     /gene="purB"
                     /locus_tag="PKN91_07360"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNS27925.1"
                     /translation="MDQNWIDALSPVDGRYRKAADPLRPILSEQGLIRERIRVEAEWF
                     RFILAEVPEFRALGVGSAVLARAGALAADPDAGAAAEVKQIESRINHDVKAVEYYVRD
                     ALAVSGATPQALELVHFGCTSEDINNVCHARLLQAARVVLLATLDARLATLRTLAREY
                     ASLPMMARTHGQTASPTTLGKEFANVVARLERARRRFAAVEALGKWNGAVGNYNAHVA
                     AFPGLDWPAVSARFVASLGLGHNAYTTQIEPHDWIGEYCDALAALNVIVLDFCRDSWG
                     YISLGYLRQRAVAGEVGSSTMPHKVNPIDFENAEGNLGVANALLRHFAEKLPVSRWQR
                     DLTDSTVLRNLGVALAHTLIAWHALGRGLGKIEANPARIAADLDAAWEVLAEAIQTVL
                     RAHGVPNGYELLKEFTRGRPIDATMLHDLVGQLPLPAAARAQLLALTPAGYTGLAASL
                     AAQMDAPN"
     gene            9032..10501
                     /locus_tag="PKN91_07365"
     CDS             9032..10501
                     /locus_tag="PKN91_07365"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNS27926.1"
                     /translation="MITHAAARPMRADVGALAEYPMSSSSFRIERDSMGELQVPLEAL
                     WGAQTQRAVQNFPISGLTMPREFLRALGLVKSAAATANAELDLLPAPIARAIEAAAAE
                     VAAGRHDAHFPIDVFQTGSGTSTNMNANEVIAHLAARASGLTVHPNDHVNMSQSSNDV
                     IPTAIHVSAALAVAENLLPALAHLAQVIAGKEREVGAIVKTGRTHLMDAMPVTLGQEL
                     SGWRTQIENGRARIATGAPRLHALAQGGTAVGTGINAHPEFGARFCKVLAKLTGLPFV
                     PSPNYFESLSSQDAAVELSGQMKVVAVSLMKIANDLRWMNSGPLAGLAEIALPALQPG
                     SSIMPGKVNPVVPEATAMVAAQVIGNDTTITVAGQSGNFQLNVMLPVIAHKLLESARL
                     LANVARLLADSAIAGFTVNQPQLAAALDRNPILVTALNPVIGYEKGAAIAKKAYAEGK
                     PILAVAAAMTDLPREKLQQLLDPAELAKGGIKGGTSGGG"
     gene            10512..11171
                     /locus_tag="PKN91_07370"
     CDS             10512..11171
                     /locus_tag="PKN91_07370"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNS27927.1"
                     /translation="MSPDRSGAAGPALRERVLASLRGSLPSSSVTDWRIAGAPPEQAA
                     RLRAHFPARPVPAAVLMPIVDHPHGLTVLLTQRAEQLKNHAGQVSFPGGRIEPGDRGP
                     LDAALRETHEEIGLDAGRVHVIGYLPDHLIISGFRVTPVVGFVDPGFALRLDPCEVDA
                     TFEVPLDFLFDVQNHRSRLRRIGDEDVPVYDIPYEGHHIWGATAGMLMTLYQLVTGGS
                     R"
     gene            11189..11965
                     /gene="mazG"
                     /locus_tag="PKN91_07375"
     CDS             11189..11965
                     /gene="mazG"
                     /locus_tag="PKN91_07375"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNS27928.1"
                     /translation="MTRLLAIMAQLRAPQGGCPWDREQSFETIAPYTIEEAYEVADAI
                     AGGDPARLRSELGDLLFQVVFHARLAEERGWFSFEDVAVAIADKLTSRHPHVFADARF
                     ADASEQSAAWEAAKAAERHAAGERGTLADVPLALPALSRAAKLSRRAASVGFDWPDAT
                     GVRNKVTEELGELDAAVGTEVPARQAEELGDLLFAIVNWARHLKIDPESALRSANAKF
                     ETRFARMEALAEAAGEPLAGLDAAAWDRLWVAAKRDVKAP"
     gene            12052..12363
                     /locus_tag="PKN91_07380"
     CDS             12052..12363
                     /locus_tag="PKN91_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNS27929.1"
                     /translation="MRHFLLSLVFALGAALVSTGALADPDRHQGKQRGDRARQQQPAA
                     PGMSLDAAVRQAERKYQARVIRAETRSSQDGRVVHVLRLLSEDGRVWTVKIDAASGAE
                     S"
     gene            12371..>12839
                     /locus_tag="PKN91_07385"
     CDS             12371..>12839
                     /locus_tag="PKN91_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNS27930.1"
                     /translation="MRILVVEDEEILRESLKRELVAAGFTVDVASDGEEGLYAGREYP
                     VDLAIIDLGLPKLPGLELIRTLRAERRGFPILVLTARDRWQDKVAGLDAGADDYVTKP
                     FQFEEVVARVQALLRRAGGWASPVLSCGPIVLDLRSQTVAVNRQAVDLTTFEYR"
BASE COUNT         2053 a         4293 c         4467 g         2026 t
ORIGIN      
        1 gccgagaagg actgggatct gtatgagttc gccgagtgcc tgatcgacgt ggactacaag
       61 ttccagacct ggcgcttcca gcacatgaag accgtcgagc gcatcatcgg ctacaagccc
      121 ggcacgggcg gcacgggcgg cgtgtcctat ctcgccaagg cgctggagct caagttcttc
      181 ccggagctgt ggcagatccg tacgtcgatg tagccgccgc gcgcacccgc gcgccgcgcc
      241 gttgccacac ccgccagccg agcagcacgg ccaggatacc cgcgtacacg agcggctcgg
      301 tcacgtcccg cttcacctgc cagtagtagt gccacacccc caggatcgcg atcaggtaga
      361 cgagccggtg caggtgctgc cagcgccggc cgagcctgcg catcatgcgc tgcgtggaag
      421 ttgcggcgag cggcagcagc agcagcaggg cggtgaaccc gatcgtgatg tacgggcgcc
      481 tggtgatgtc ctcgacgacc atcgaggcgt cgaaggcgcg atcgagcgca aggtagacgg
      541 cgaaatgcag cgccgcgtac gcgaacgcga acagccccag catccgccgc aggcgtatca
      601 gctgcggccg gtgcgcgatt ctcgcgagcg gcgtcaccgc gagcgtcagc agcagcaggt
      661 tgagtgcggt cttgccgcag gtgagcagca gttcctcgac cggatcggcg ccgaggcgct
      721 ggcctgcgat gccgaacacg ccggcaagca tggacagtgc cggcagcagc gcggcgatga
      781 acacgagcgg tttccagaca tagcggtaac gctgctcgac gcgcatcgtg gcccggctca
      841 gaacaggcgc cgcaggtcca tgccacggta gagtccggca acttccgcgg cgtagccatt
      901 gaacggctct gtggggcgtg tcgcatcgaa gaggcccgcg ccgatgcggc gctcggtcgc
      961 ctggctccag cgcggatggg cgaccgccgg attcacgttc gcgtagaagc cgtactcgcg
     1021 gggagccgcg atgttccagc tcgtcggcgg catggttcgg gtgaaggtga tgccgacgat
     1081 cgacttgatg ctcttgaagc catatttcca cggcaccacg agccgcagcg gcgcaccgtt
     1141 ctggttcggc agcacccggc catagaggcc gacggcgagc agcgcgagcg ggtgcatcgc
     1201 ctcatcgata cgcaggccct cgacataggg ccactcgagc cagccggcac tctggccggg
     1261 catctgccgc ggatcgtgca gcgtcctgaa ggcgacgaac ttcgcgtcgg cggtgggctt
     1321 gaaccggcgc agcagatcgc cgagcggaaa gccgacccac ggaatcacca tcgaccatcg
     1381 ctcgacacag cgcaggcggt agacgcgctc ctcgagtgcg tgcggcttca ggatgtcctc
     1441 gagcgtgaag cttccgcgcg tttcggcttc gcccgcgatc gtcacgctcc acggccgcgt
     1501 gcgcagagtc gcggcattgc gctgcggatc gcccttgccg gtgccgaact cgtagaaatt
     1561 gttgtacgaa gtgatgtcct cgtaggcggt ggtcgtttca gccaccgagt agcgggcgtt
     1621 gcgtttcgcg gcgagcttcg cgagcggtgg cgcttcggcc gcatcgcccg cgcctgcgaa
     1681 ggcgaggctg gcggtggccg cggcgatcca gcgacgccgg tcgaggtaga ccccgggcgg
     1741 cgtgatctcc gagggtggcg gttgatgata tttaaatatc aatttgttac gtgatattgc
     1801 taatgcgttt tagcattact tgcgggccga tccgaaggag ggcgcctgtg catttggagt
     1861 gcgtggcgcg aatcatgttc cccgccagtc cacacattcg atgccaagca cggccgcctc
     1921 ggcccgcgcg cggcgcggac cgttcaccag cacgccccgt tcagcgaggc gcatatgggg
     1981 aaggtccgag ctgccattgc cataggccgt gatgctcgcc accggggcga gatcggcacg
     2041 caggcgctcg aggcagcggg ccttctcctc gcctcgccgg ttgggcgtcg cgagcgcacc
     2101 gtcgagcacg ccatcctccc agcgcactcc ggtgcagacc acgtgatcga agccgaggag
     2161 tttgcccagc gccggcacgt acaggtcgac gctcgccgac atcagcacca gcgtagcgcc
     2221 gcgctggcgc tcgcgggcga gcgcggcgac ggcacccggc agcaggtcac gcgcgatcac
     2281 tcgcggcagg taggctgcat tccaggcctc gatcgccgcg cgcgtctccc cgcgcagcgt
     2341 gtgccgcagg agcgccgcct tcagccggcc gcgatcgcgt ccggcgaaca ggtaggcgag
     2401 gagagcgggt acgacggcga gcagcctcgc gagacgccat gggcgtcgcg cgaggtagcg
     2461 ccagacatac ggaaagagcg tgtcgcggcg tgtgatcgtg ccgtcgagat cgaagaccgc
     2521 gaccacacgc ccgacggtgc tcatcaggcg gcggcgcggc tcgctccggc gagctgtcgc
     2581 agtacgtact gcaggatgcc gccgtgccga tagtagtcgc gttccttcgg cgtatcgatg
     2641 cgcacgcgcg cctcgaaggt gatcggcttg ccgccgtcga gcggcgtggc caccactttc
     2701 accgtcttcg aggtctcgtc cgcgaccccc acgatgtcga tgacttcacg gccagtgagc
     2761 cccaggctcg cggcgctctc gcccgcgggg aactgcagcg gcaacacgcc catgccgatc
     2821 aggttcgaac ggtggatgcg ctcgaagctc tcggcgatca cggccttcac gccaagcagc
     2881 atcgtgcctt tcgcggccca gtcgcgcgag gagccggtgc cgtattcctt gcccgcgagc
     2941 accacgagcg gcgtgcgctc cgtcctgtaa cgcatggcgg cgtcgtagat gctcgattgc
     3001 tcgcccgacg gcagatgcac ggtgacgccg ccctcggtgc ccggggccag caggttgcgc
     3061 aggcggatgt tggcgaaggt gccgcgcatc atcacttcgt ggttgccgcg gcgggaaccg
     3121 taggagttga agtccttctg ctccacgccc tgctcggtga ggtagcgggc agccgggctc
     3181 gacttggcga tattgccggc cggcgatatg tggtcggtcg tgaccgagtc gccgagcagc
     3241 gccagaacgc gcgcgccctt gatgtcgctg accgcctcgg gctgcatggt catgccgtcg
     3301 aagtagggcg gattcttgac gtaagtggac ctgtcatccc acttgtagat cttgcccgcc
     3361 gggaccttga tgctcgacca atgctcgtcg cccgcgaaga cgccctcata gctcttgcgg
     3421 aacatgcccg aatcgatcgc gcgctgaacg gtgtcggcca cttcatgatc ggccggccag
     3481 atatccttca ggtagaccgg cttgccgtcg gcatcgttgc cgagcgcatc ggtggtgagg
     3541 tcgatgtcga gcgtgccggc aagtgcgtag gcgaccacca gcggcgggga ggccaggaag
     3601 ttcatcttca cttccggatg cacgcgcccc tcgaagttgc ggttgcccga cagcactgac
     3661 gtcgcgacga tgtcgccggc cgccacggcc gccgaaatct ccggtcgcag cggcccggag
     3721 ttgccgatac aggtggtgca gccgtagccg acgagataga agcccgcgct ggcaagatcg
     3781 tcgagcagct cggccttgcg caggtagtcg gtgacgacac gtgaaccggg cgcgaggctc
     3841 gtcttgaccc agggcttggc ggtgagtccg cgccgcgccg cattgcgcgc gagcaggcct
     3901 gcgccgatca tcacgtaggg gttcgaggtg ttggtgcagc tcgtgatcgc cgcgatcagc
     3961 acggcaccgt cgctcaactc gaagctcctg ccatcgagcg tgacctgcgc gccgcccgtc
     4021 gcgccggact tcctggcccg ctcctcggcc atcggcttca gcgccgtgcg gtaggcggcc
     4081 tgcgcgctgc gcagcggcac gcgatcctgc gggcgcttgg ggccggcgag gctcggctcg
     4141 acgctcgcga ggtccagttc gagcacgtcg gtgtactgcg cgtccggctg gccgttctcg
     4201 cgccacaggc cctgtgccct ggcgtaggcc tcgaccagcg cgatctgctc ttcgggacgt
     4261 cccgacagcc gcaggtagcg cacggtctcg gtgtcgatcg ggaagatcgc gcaggtgctg
     4321 ccgaactcgg gagacatgtt gccgagcgtc gcgcgatcgg cgagcggcag gttggcaagc
     4381 ccgtcgccaa agaactcgac gaacctgtcg accacgccac gcttgcgcag catctcggtg
     4441 accgtgagca cgaggtcggt ggcggtcgca cccggccgca ggctgccgac caggcggaaa
     4501 ccgatcacct gcgggatcag catcgggatt ggctggccga gcatggccgc ctccgcctcg
     4561 atgccgccca caccccagcc gagtacgccg aggccgttga tcatcgtggt gtgcgaatcg
     4621 gtgccgacga cggtgtcggg gtaggcctgg cgcacgccgt cacgctcggc gtcgaagacc
     4681 acacgcccta ggaactcgag gttgacctgg tgcacgatgc cggtgttcgg cggcacgacc
     4741 ttgaagttcc tgaacgcagc ctggccccag cgaaggaacg cgtaacgctc gccgttgcgc
     4801 tcgaactcga tgcggttgtt ctcctcgagc gaacgcggcg tgccataggc atccacctgc
     4861 accgagtggt cgatgacgag ttcggcgggt gccagcggat tgaccatctc ggggtcaccg
     4921 ccgagcctcg tgatagcctc gcgcatcgcg gcgaggtcga ccacgcaagg cacgccggtg
     4981 aagtcctgca tgatcacgcg cgccggcgtg aacgcgattt cgtgcgaggg cgcggactgc
     5041 gggtcccaat cgagcacggc ctcgatgtcc ttgcgggtca cgttgacgcc atcttcgaag
     5101 cgcagcaggt tctcgagcag gatcttgagc gagtagggca ggcgcgcgac cttctgctgc
     5161 ggcagcgcct tgaggctcat gatttcgtag gtgcgtgatc ccacggcgag ctgcgtgcgc
     5221 gccttgaagc tgtcggtcat cgtgttctcc gcgaggggcg agtggagtca aatcagccga
     5281 caattatagc ggctgcaggc cccgcgtgcg actcggcacc cacgggccgg gtcgagtcga
     5341 tcacccctcc gccgatgcag cgcgtgccct ggtagagcac cgcatactgg cccggcgcca
     5401 ccgcacgctg cggctggtcg aagttgagcc ggacccggcc gcccgcgagc ggcatgacgt
     5461 ccacgggttg gtcggcctga cggtaacgca ctttcgccat ggcccggaat gccccgcccg
     5521 gaacacgcgc gagccagtga aaggcttcgg tgaccagcgc tgtcgactgc agcagcggat
     5581 gatcgtggcc ctgcacgacg atcagggcat tgcgtgcgac atccttgcgc gcgacatacc
     5641 acgcgccctc gtcgtggccc gactggccgc cgatttcgag gccgccgcgc tgcccgagcg
     5701 tgtagaacgc gagcccccga tgcgtgccga tgcgctcacc atccgccgtc tcgatgggac
     5761 cgggttccga cacgatgtac tgcgagagga attcgcgaaa cggccgctcg ccgatgaagc
     5821 agatgccggt gctgtccggc ttgtcgtaga ccggcaggcc cgctcggcgc gcccgctcgc
     5881 gcacctcgtc cttgtacagg tcgccgatcg ggaaggcgat cgagccgagc gcttcgggtt
     5941 cgatcgaatg caggaagtag gactggtcgc gtgccggatc ggtgcctttg tgcagctcga
     6001 tcccctccgg acccgcgacg aggcgcgcgt aatgccccgt ggcgtagcgg ctggcgccga
     6061 ggcgacgggc gtgcgcgagg cagatgccga acttgatctc gcggttgcag gcgacgtccg
     6121 ggttcggcgt gcgtcccgcg gagtactcgg cgaggaagtg cgcgaacacg cgttcgcggt
     6181 actcgcgcct gaagtcgacc cgatgcaggg cgatgccgag cacttcggcc accgcgcgcg
     6241 catcctggaa atcctgcgcg gccgtgcagt agccgtcgtc gtcgtcccag ttggtcatga
     6301 acaggccctg cacgtcgtgg ccgtcctcga gcaggcgcag cgcagcgacg gcggaatcga
     6361 ctccgccgga caggcccacg atgatcgtgt cgcgcgcact catgcgcgca ttgtacgtcg
     6421 ccgggaaaag tggcttgggc tcagccgacc ttgaccgcgc gcatgtgcac ggcggcctgc
     6481 aggtcgaggt gcgcgagcga tgcgagcggc aggcgctggc cggcaagata gtcgtcgatg
     6541 cagcgaagca ccagcgggct gcgcaggcgg gtgtgctcgg cttcgagctc ctcgcgtgtc
     6601 agccagtgcg tgccgactat gccgcgatcg aggggcgcgc gcgggtcatg gtctgagact
     6661 tcgccactga aggcgatgcg caaggtcgat ttgccggtgc gggcattgcg ccacaggtag
     6721 atgccgagga catccgccgg gacgaagcgc caggccgatt cctcgcgggt ctcgcggatc
     6781 accgcttcga gcagcgtctc gcgatcctcg acatggccgg caggctggtt cagcacccgc
     6841 tgcccgcgga tgctttcctc gacgagcagg aagcggccgt cgcgctcgac gacggcggca
     6901 acggtgacgg cgggacgcga acccatctca gcgacgcgcg acgcggtagc cgaagtcggg
     6961 tgcgtgggtg acccactgcg agtcgaagtc ggcgaggtgc atgcgcgcta ccgcaggatt
     7021 gtcgagatcg gcaatgccgt cccactgggc ggcgttggtg cgatagagga tggcgcgatc
     7081 atcagcgatg atgtaaccgg ggtgcggctt cggcgcatcc tcgccgagca cacggatgtc
     7141 gatgtagctc gtgagacgcc ggctcatggc cacgaagcgg tgcacgtcgc ggatcatgcg
     7201 gctgccgtcg ccgagcagga cgcgcacctt ggcaaagctg cgcgcgagca cgaagcgctt
     7261 gacgatgtcg aggaagggtg tctggtcgta gaggtcgggc tcgaggttcg gggtgtagat
     7321 cgacatcagg cgctgggcac tgccggcgac gaggatcgtt gccgcgagca cttcctcctg
     7381 ggtcgtcacc acattgacct gcggcggcct ttcgagtggc ggcagctcga gacccttcgc
     7441 gggcgtctgc gacagcaggc tcaactcgaa ctccccagtg ctgctcacga tggtcttccg
     7501 cagtcccggc gcaaagtgcg caccggcggg attctccggc agtccgtgcc ggccgcgtgt
     7561 cgatgcggtc acgttttggc cccgctttgc acaggccgtg ccccgaaagc gtgccgcagt
     7621 tctcagttcg gtgcgtccat ctgcgccgcg agtgacgcgg cgagtcccgt atagccggcc
     7681 ggggtgaggg ccagcaactg cgcgcgcgcc gcggccggca gcggcaattg tcccacgaga
     7741 tcgtgcagca tcgtcgcgtc gatcgggcgt ccgcgcgtga actccttcag cagttcataa
     7801 ccgttcggga cgccgtgggc gcgcagcacg gtctggatcg cctcggcgag cacttcccac
     7861 gccgcgtcga ggtcggcggc aatcctcgcc ggattcgcct cgatcttgcc gagaccccgc
     7921 ccgagcgcat gccaggcgat cagcgtgtgc gcgagcgcca cgccgaggtt gcgcagtacc
     7981 gtcgagtcgg taaggtcgcg ctgccagcgc gacaccggca gtttctccgc gaaatggcgc
     8041 agcaatgcgt tcgcgacgcc gaggttgcct tcggcgttct cgaagtcgat cgggttgacc
     8101 ttgtgcggca tggtcgacga gccgacctca ccggccaccg cgcgctggcg caggtagccg
     8161 agcgagatgt aaccccagct gtcgcggcaa aagtcgagca cgatcacgtt gagcgccgcc
     8221 agcgcgtcgc agtactcgcc gatccagtca tgcggctcga tctgcgtggt gtaggcgttg
     8281 tggccgagac ccaggctcgc cacgaaacgc gcgctgaccg ccggccagtc gagcccggga
     8341 aacgccgcga cgtgcgcgtt gtagttgccc accgcgccgt tccacttgcc gagcgcctcg
     8401 acggcggcga agcgccggcg cgcgcgctcg agccgcgcga cgacattggc gaattccttg
     8461 ccgagggtcg tgggactcgc ggtctggccg tgcgtgcggg ccatcatcgg cagcgacgcg
     8521 tactcgcgtg ccagcgtgcg caaggttgcg agccgcgcgt cgagcgtggc gagcagcacc
     8581 acgcgcgcgg cctgcagcag gcgcgcatgg cagacattgt tgatgtcctc ggacgtgcag
     8641 ccgaaatgca cgagttcgag cgcctgtggc gtggcgccgg agacggcgag ggcatcgcgc
     8701 acgtagtact cgaccgcctt gacgtcgtgg ttgatgcgcg actcgatctg cttgacctcc
     8761 gctgccgcgc cggcatcggg atcggcagcc agcgcgccgg cgcgtgcgag caccgcgctg
     8821 ccgacaccga gcgcccggaa ctccgggacc tcggcaagga tgaagcgaaa ccattccgcc
     8881 tcgacgcgaa tgcgctcgcg gatcaatccc tgctcgctca ggatcgggcg gagcggatcg
     8941 gcggccttgc gatagcggcc gtctaccggc gacagggcgt cgatccagtt ctgatccatg
     9001 cgagcgggtc accgggaatg ataagatgca catgataacg catgcggccg cccggccgat
     9061 gcgcgccgat gtcggcgcac ttgcggagta cccgatgagc tcttcgtctt ttcgtatcga
     9121 gcgtgacagc atgggcgagt tgcaggtccc cctcgaggcc ctgtggggcg cgcagacaca
     9181 gcgcgcggtg cagaacttcc cgatcagcgg cctcacgatg ccacgcgagt tcctgcgtgc
     9241 gctcggcctg gtgaaatccg cggccgccac cgccaatgcc gaactcgacc tgctgcccgc
     9301 gccgatcgca cgcgcaatcg aggctgccgc ggcggaagtg gccgccggca ggcacgatgc
     9361 gcatttcccg atcgatgtct tccagaccgg atcgggcacg agcaccaaca tgaatgccaa
     9421 cgaggtgatc gcccacctcg ccgcgcgtgc ctcgggcctc accgtgcatc caaacgatca
     9481 cgtcaacatg agccagagca gcaacgacgt gattcccacc gcgatccacg tcagcgcggc
     9541 gctggccgtc gcggagaacc tcctgccggc gctcgcgcac ctggcgcagg tgatcgcggg
     9601 caaggagcgc gaggtcggcg cgatcgtcaa gaccggccgg acccatttga tggatgcgat
     9661 gccggtcacg ctcgggcagg aactgtcggg ctggcgcacg cagatcgaga acggtcgcgc
     9721 gcgcatcgcg accggcgccc cgcggctgca cgcgctcgcg cagggcggca cggcggtcgg
     9781 caccgggatc aatgcgcatc cggaattcgg cgcgcgattc tgcaaagtgc tcgcaaaact
     9841 caccgggctg cctttcgtgc cgagccccaa ctatttcgaa tcgttgtcgt cgcaggacgc
     9901 ggccgtcgag ctctccgggc agatgaaggt cgtggcggtg agcctgatga agatcgcgaa
     9961 cgacctgcgc tggatgaaca gcgggccgct cgcgggactc gccgagatcg cgctgccggc
    10021 gctgcagccc ggcagcagca tcatgcccgg caaggtgaac ccggtggtgc ccgaggcgac
    10081 cgcgatggtt gcggcgcagg tcatcggcaa cgacaccacc atcacggtcg ccggccagtc
    10141 gggcaatttc cagttgaacg tcatgttgcc ggtaatcgcg cacaagctgc tcgagagtgc
    10201 gcggctgctg gcgaatgtgg cgcggctgct ggcggattcg gcaatcgccg ggttcaccgt
    10261 gaaccagccg caactcgccg cggccctcga tcgcaatccg atcctggtca cggcgctcaa
    10321 tcccgtgatc ggctacgaga aaggtgcggc gatcgccaag aaggcgtacg ccgagggcaa
    10381 gccgatactc gccgtggccg ccgcgatgac cgacctgccg cgcgagaagc tgcagcaact
    10441 gctggacccc gccgaactcg ccaagggcgg catcaagggc ggcacgagcg gcgggggctg
    10501 agcggcaggg catgagcccg gatcgatccg gcgcggcggg cccggcactg cgcgagcgtg
    10561 tcctcgccag cctgcgcggc agcttgccgt ccagcagcgt cacggactgg cgtatcgccg
    10621 gcgcgcctcc ggagcaggcc gcgcgcctgc gcgcgcactt tccggcacga cccgtgccgg
    10681 ccgccgtcct gatgccgatc gtcgatcacc cgcacggact caccgtgctg ctgacgcagc
    10741 gcgccgagca gctgaagaac cacgccggcc aggtcagctt tcccggcgga cgcatcgaac
    10801 cgggcgaccg cggcccgctc gacgccgcgc tgcgcgagac ccacgaggag atcgggctcg
    10861 atgcggggcg cgtgcacgtc atcggctacc tgcccgatca cctgatcatc agcggcttcc
    10921 gcgtcacgcc ggtggtcggc ttcgtcgatc cgggtttcgc gctgcggctc gacccgtgcg
    10981 aggtggacgc gaccttcgaa gtgccgctcg atttcctctt cgacgtgcaa aaccaccgat
    11041 cacgcctgcg tcgtatcggc gacgaggacg tgccggtcta cgacatcccc tacgagggtc
    11101 accatatctg gggcgccacg gccggcatgc tgatgacgct ctaccagctc gtcaccggcg
    11161 ggtcgcggtg agcggcggga atgcagcggt gacccggctg ctcgccatca tggcgcagtt
    11221 gcgtgcgcca cagggcggct gcccctggga tcgcgagcag agcttcgaga cgattgcccc
    11281 ctacacgatc gaggaagcct acgaggttgc cgacgcaatc gcgggtggcg atccggcgcg
    11341 cctgcgcagc gagctcggcg acctgctgtt ccaggtcgtc tttcacgccc gcctcgccga
    11401 ggagcggggc tggttttcgt tcgaggacgt cgcggtggcg atcgccgaca agttgaccag
    11461 ccgacatcct cacgtgttcg cggacgcgcg gttcgcggac gcgagcgagc agtccgcggc
    11521 ctgggaggcc gcgaaggcgg cagagcggca tgcggccggc gaacgcggca cactcgccga
    11581 cgtgccgctc gccttgccgg cgctctcgcg cgctgccaag ctcagcaggc gcgcggcaag
    11641 tgtcggcttc gactggcccg acgcgacggg agtacgcaac aaggtgaccg aggaactcgg
    11701 cgagctcgat gccgcggtcg gcacggaggt ccccgcccgc caggcggagg agcttggcga
    11761 cctgctgttt gccatcgtga actgggccag gcacctcaag atagatccgg agagcgccct
    11821 gcggtcggcc aatgcgaagt tcgagacccg ttttgcgcgg atggaggcgc tcgcggaggc
    11881 agccggggag ccgctggccg ggctcgatgc cgccgcctgg gaccgcctgt gggtcgccgc
    11941 gaagcgggac gtgaaggcgc cgtgaagcgc gcttcaggac cggttcagtt gcgcacccct
    12001 aagcttgcgg gcgaccgtgg cgcttcgcag ggacgaacag actggccggt gatgagacat
    12061 tttctgcttt cgctcgtatt cgcgctcggc gccgcactgg tctccaccgg agcgctggcc
    12121 gaccctgacc gccaccaggg caagcagcgc ggtgaccggg cccgccagca gcagccggcc
    12181 gcccccggca tgtcgctcga tgccgcagtc aggcaggccg agcgcaagta ccaggcgcgc
    12241 gtgatccgcg ccgaaacccg tagctcccag gacgggcgcg tcgtgcatgt cctgcgcctg
    12301 ctgtcggagg acggtcgcgt ctggaccgtg aagatcgacg cagcgagcgg cgcggagagt
    12361 taggcacccg atgcgcatcc tggtcgtcga agacgaagaa atcctgcgcg aatcgctcaa
    12421 gcgcgaactc gttgcggccg gcttcaccgt cgacgtcgca tccgacggcg aggagggcct
    12481 gtacgcgggg cgcgagtacc cggtcgatct cgccatcatc gatctcggcc tgcccaagct
    12541 gccgggcctc gagctgatcc gcacgctgcg cgccgagcgc cgcggctttc cgatcctcgt
    12601 gctgaccgcg cgcgatcgct ggcaggacaa ggtggcgggc ctcgatgccg gcgccgacga
    12661 ctacgtcacc aagcccttcc agttcgagga agtcgtcgcg cgcgtgcagg cgctgctgcg
    12721 ccgggcgggc ggctgggcca gccctgtcct cagctgcggt ccgatcgtgc tcgatctgcg
    12781 ctcgcagacc gtggcggtca accgtcaggc ggtggatctc accaccttcg agtatcgcc
//
LOCUS       DAOQVY010000064        12783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_5531_length_12783_cov_5.899827, whole genome shotgun sequence.
ACCESSION   DAOQVY010000064 DAOQVY010000000
VERSION     DAOQVY010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12783
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5531_length_12783_cov_5.899827"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PKN91_07390"
     CDS             <1..587
                     /locus_tag="PKN91_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNS27931.1"
                     /translation="LINDIAEQTNILALNASIQASMAGEAGRGFAVVADEVQRLAERS
                     ALATKQIEVLVRTIQTDTNEAVVSMERSTTDVVGGALLAENAGAALEEIEQVSNQIAS
                     LVQNISSSSREQASSAQSIARNLQVLREISAQTAESTSANSVAIAKLAELSAGMRKAA
                     AGFRLPGAPDPGLTMRGLKRPEVPAELARRVKAS"
     gene            592..6066
                     /locus_tag="PKN91_07395"
     CDS             592..6066
                     /locus_tag="PKN91_07395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNS27932.1"
                     /translation="MSEIATQTLELVGREINATLGEARASVEGFVEQPDNVALLERVA
                     GDLHQVQGVLRVLEIYGAALLAEEMEQVTRYLIATSAERKNQAESLDALLRAMVQLPG
                     YLERVIAGGRDLALVLLPLLNDLRAVRGSPLLSEGTLLLLNLKSDRQATPVAPSPGEQ
                     PLTIEQWARRLRARFQVGLIGWIRGERIDQNLEILAAVAHKIEQVATRQPVFQLWWVT
                     GALIEALRERGVEGGVSIKRLLGLADREIKRLYEEGEARYCQSPPVELLNNILYYIAR
                     AESSGPKVSAVRASFRLGDLLPVDEAVEQERESLSAPSVKLMRTVGAAIREDLAKVKD
                     VLDIFVRRGGGQAAELAPQVELLRKIGDTLGVLGLGELRTRVQGEIGRLEAIVSGQAQ
                     VDDGTLLEIAATLIGIEDHLDDQLVGMIVPKARDASQGADDQDFHQVQAAVLRECMLN
                     LARVKEAVAQSVGGTLGTAGLDSWSELMRGIKAGLVMLGKMRAVEIVDGIGNHLQRVM
                     RPGVPALPSHLLDRLADAIVSVEYYMETLQARRSEPFYMLDNAQACLDALAAEPEPAV
                     PTVPPLGETTFARTLRIAPPTTASGAYRVVHGTDGGDFDATDAPAASPPTLAPVPVTP
                     PTSALADPELVGLFIEEAGEEVARIQREFPRWEENPLEDGALSIVRRSFHTLKGSGRM
                     VGAREMAEFAWAIENLLNRLIDRTLGRSPEILATLRDGVRALPQLLVALESGSACALD
                     TAPIIARAHALAAHQDTDAARGEGGETTSAVQVPETALKAAAPVPDADPGLQEIFTRE
                     TAAHIATVRSFLGEESHRPEPHVLTEDVYRACHTLSGSSKAAVARHGIRLAGPLDHWM
                     RKSFDSGVGLTSADLALVGDCMQEMERVAGHLDEGTGFFQTHDQLRARIARAETDLDK
                     RIAEAAEAAEAEQAASLPPQAPPVSAQPPGPPEPAVSADYDPEIAGIFAEEAGELIES
                     AERALQSWGADRASGAHLDALKRPLHTLKGGARMAGVIAMGDLAHEMETLVNQMESGT
                     VRADESAQAVLVASLDEIAHMRDAIIAGRGVARADALIARIHALDPSRAAAAPAAPAA
                     APAAAAAAVPAVAAAPEPEPRAEPEEIEIELPSSLEGDAGIETPVDLSPPAMPIEPPA
                     PIAPVALAAPAAVAAPAAPTAPVVMTPAIAPPPTFGPPTAAGNGEPLAPPAPVPPGRE
                     PAARGERQEMARVDADLLDRLLNYSGEVSIGRARLEQQIASIDFNLAELSRTVTRLKE
                     QLRKLEIETESQILHTHEDEAGHRAEFDPLELDRYSTIQQYSRGLAETASDVASIQQL
                     LESLTQDTQNLLQHQARTITELQNGLMRTRMVPFERHVQRLARIVRQAAFDTRKRAEL
                     LVEGAAGELDRQVLERMLPAFEHILRNCVAHGIESPDEREARSKPATGRITLALRREG
                     AEVIVQVSDDGAGMNLKAIREKGQALGLIRPGQVLSDEDVMQLVLEPGFSTAGKVTQS
                     AGRGVGMDVVADTVKKLGGALHMETKEGEGTSFTIRLPFTLAISHALVVRAGDEFYAL
                     PLTTVEGVVRLTKDEVTAHLGPEAPQFEHNGQRFRFQHLAQYVGLEPSVLPEQDVTIP
                     VILVRAGEHSTGLVTDELVGSREIVVKSVGPQISAIRGISGATILGDGRIVVILDINA
                     LVRAEWRGRSEPVVPRDRSDKRTLALVVDDSITVRRVTQRLLERNGMRVLTARDGVDA
                     IEVLQENQPDIILLDIEMPRMDGYEVAAHVRADERLKEIPIIMITSRVGEKHRARAIE
                     IGVDDYLGKPYQEAQLLEAIEPLVSRRREPERASGSGRGH"
     gene            6070..6543
                     /locus_tag="PKN91_07400"
     CDS             6070..6543
                     /locus_tag="PKN91_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNS27933.1"
                     /translation="MAERVSEIYSLLIPLVDGRLIVPRACVAEVVGFQPPAEMTGAPP
                     WYLGTVPWNGRTVPLICFEGTCGQGIPPATGRSRIVVFHCLGSRVEGGYFGLVSQGFP
                     QLVRVSSDVVRPDNTRSFPERHPVICQVRMVNEAPLVPDIEMLEEMIADETSVAA"
     gene            complement(6619..6948)
                     /locus_tag="PKN91_07405"
     CDS             complement(6619..6948)
                     /locus_tag="PKN91_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS27934.1"
                     /translation="MVTLRKRKSVAGPPRAARMVEDIVGCKWSLVVLDLVGQGVARPG
                     AMQRQVPGLTAKVLNERLRKLLRFGLIERQVFAEVPPHVEYRLTTLGRRFGDILERIK
                     ALDRELG"
     gene            7066..7557
                     /locus_tag="PKN91_07410"
     CDS             7066..7557
                     /locus_tag="PKN91_07410"
                     /inference="COORDINATES: protein motif:HMM:NF028004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HNS27935.1"
                     /translation="MNVRHLAAAAALAGVIAAGAVVGAEDDAVPYPDGYRAWQHVKSM
                     VIGEGHPLYGSFGGIHHLYANERAIAGYTGGHFPDGAVIVFDLLEAVAADGAITEGQR
                     KVVGVMHRDAKKYAATGGWGFEGFAGDSRSERVVGAGNGAGCFGCHAPQKSQEFVFSS
                     YRP"
     gene            complement(7545..8300)
                     /locus_tag="PKN91_07415"
     CDS             complement(7545..8300)
                     /locus_tag="PKN91_07415"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNS27936.1"
                     /translation="MRLVRVYSAEPLAANREHTLSRSASDHVLRVLRLHAGDPLVLFD
                     GRGGEYPARLVGAGKGAAVVATGDHRGIERESPLAITLLQGLARGEKMDLVVQKATEL
                     GIARIVPVALGRSVMRVADGERADRKLAHWQAVAVSACEQCGRNRVPEILAPMAFAAA
                     LALAADIGQRLLLAPSAEQDLGAMELTAAPLALLIGPEGGLEDDEIVTAARQGWRAAR
                     FGPRVLRTETAAIAALAVLQARAGDLGGRDQGR"
     gene            8326..8880
                     /gene="ampD"
                     /locus_tag="PKN91_07420"
     CDS             8326..8880
                     /gene="ampD"
                     /locus_tag="PKN91_07420"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HNS27937.1"
                     /translation="MLLDIDAATGLLRGVRQVLSPFFDPRPAGMTPDLIVVHNISLPP
                     GEFGGPWIDALFTGGLPPGRHPYFETLKGVRVSAHALIRRDGSVTQYVPFAQRAWHAG
                     KSVYHGRTDCNDFSIGIELEGTDDVPFEGAQYTTLADLIRALLAIYPTLTRESITGHS
                     DIAPGRKTDPGPAFDWPRLRALIA"
     gene            complement(8891..9445)
                     /locus_tag="PKN91_07425"
     CDS             complement(8891..9445)
                     /locus_tag="PKN91_07425"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004749690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNS27938.1"
                     /translation="MGNRLSKIYTRTGDDGTTGLGDGTRVSKDDPRVAAYGTVDETNS
                     AIGAVLAHAGVPETIRRCLTEVQHDLFDLGGELCIPGARVITGEHTVRLEDDLDRFNE
                     DLPRLKEFILPGGGLAAAACHVARAVCRRAEREVWTLASASDVAPEVPRYLNRLSDLL
                     FVIARVLARAEHGTEVLWRHDRGR"
     gene            complement(9456..11330)
                     /locus_tag="PKN91_07430"
     CDS             complement(9456..11330)
                     /locus_tag="PKN91_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS27939.1"
                     /translation="MSLVFRAAQVAVIVSVAVATAPSTLALAADPALDETVVTATRTA
                     VALDELIAPLFVIDREEIERSLAPDAGELLRAHAGIELARTGGPGQQTSMFTRGTDSN
                     HTVVLIDGVRVNPGTIGSAALQNIAPESVQRIEVVKGPRSTLYGSDAIGGVVNVRTRA
                     AAQRGLTGYLSGGRYETVNGGLAGGWRIGQSAGLGFGVDYRESAGFPTLVGDTTDRGF
                     ENLTVNLAAEYSPAATLDLRARAWRAAGNSAYTTTVFDPVTFASSLAAVDQDFENASY
                     AIESLWRARSNVDVRATLTRIEDEIEQQQNNAGVAAPPYDFLRTARDGLELQGDIRLG
                     DTNELTLGALLTREDTEALSYGTAFDIGTDVNQFFVQDRAVFGRQSLVAAVGLVDHET
                     FGEELVWNAEYGFLLPTATRVTLAAGRAFRAPDSTDRYGFGGNPDLEPEVSEQIEIGL
                     RQAIGTRHSLYANAFRNDIDALVVFVFDPVTFDGRNENVERARIEGVELGYAFRGEKW
                     QARAEATLQDPRNRDTGERLLRRARENYVLALERVGARFSFGVDVSHAGDRHDTAFPA
                     TVTLDSYVLVNASARFALSDSWSVQARLANAFDEGYTLVNGYNTAGRSLTLATRFTFK
                     "
     regulatory      complement(11374..11610)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            11616..12383
                     /locus_tag="PKN91_07435"
     CDS             11616..12383
                     /locus_tag="PKN91_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNS27940.1"
                     /translation="MSLAHELAAAQEAARAAADVINALYRRNLAITVKADRSPVTEAD
                     VRAEEAIREVLTRRFPAYGFYGEETGQSAMGAESIWLVDPIDGTKSFIRDCPFFSTQI
                     ALLRAGQLVLGVSSAPAYGELAWAALGEGAFLDGQPVRVSATGALADAIVSTGNLKSL
                     ARSPRGWAGYGQLVTQVSRIRGYGDFVHYHLLARGALDLVIESDVNILDIAALTVIVR
                     EAGGVFTDLEGGEVGLATTSVLAGNAALHAAVLPLFA"
     gene            complement(12389..>12783)
                     /locus_tag="PKN91_07440"
     CDS             complement(12389..>12783)
                     /locus_tag="PKN91_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS27941.1"
                     /translation="SCVGLDLAGPRKMATLSGGEQRRVAIAALLAQQPGLFLLDEPTN
                     HLDPHHQLAVLDLFREQTHGGCAVVATLHDPTLAARYADRVVLLYGDGRWLAGEAPDV
                     LTTENLSALYLAPMLTAEVEGRRVFVMA"
BASE COUNT         1989 a         4446 c         4436 g         1912 t
ORIGIN      
        1 agctgatcaa cgacatcgcc gagcagacca acatcctcgc gttgaacgcc tcgatccagg
       61 cgtcgatggc gggtgaggcc ggccgcggat tcgcggtggt cgccgacgaa gtgcagcggc
      121 tcgccgagcg ctccgcgctc gccaccaagc agatcgaagt cctggtgcgc acgatccaga
      181 ccgacaccaa cgaggcggtc gtctcgatgg agcgcagtac caccgacgtg gtgggaggcg
      241 cgctgctcgc cgagaacgcg ggcgccgcgc tcgaggagat cgagcaggtg tcgaaccaga
      301 tcgcgagcct ggtacagaat atttcgagca gctcgcgcga acaggcgagt tcggcgcaga
      361 gcatcgcgcg caacctgcag gtgctgcgcg agatcagtgc gcagaccgcc gaatcgacca
      421 gcgccaactc ggtcgccatc gcgaagctgg ccgagctctc ggccggcatg cgcaaggccg
      481 ccgcgggctt ccggttgccg ggggcgcccg acccgggtct cacgatgcgc ggcctgaagc
      541 gtccggaagt tcccgcggaa cttgcgcgcc gggtcaaggc gagctgagcc catgtcggag
      601 attgccaccc agacccttga actggtcggc cgcgagatca atgccacgct cggcgaggcg
      661 cgcgcgtccg tcgaaggctt tgtcgagcag ccggacaatg tcgcgctgct cgagcgtgtc
      721 gccggcgatc tgcaccaggt gcagggcgtg ctgcgcgtac tcgaaatcta cggcgcggcg
      781 ctgctcgccg aggagatgga gcaggtcacg cgctatctca tcgcgacttc cgccgagcgc
      841 aagaaccagg ctgaatccct cgacgcgctg ctgcgcgcca tggtgcagct gcccggttac
      901 ctcgagcgcg tgatcgccgg cgggcgcgac ctcgcgctcg tgctgctgcc gctgctgaac
      961 gatctgcgcg ccgtgcgcgg cagcccgctg ctgtcggaag gcacgctgct gctgctcaac
     1021 ctgaagtccg atcggcaggc gacgccggtt gcgccgagcc cgggcgagca gccgcttacc
     1081 atcgagcaat gggcgcggcg cctgcgcgcg cgcttccagg tcggcctgat cggctggatt
     1141 cgcggcgagc gcatcgacca gaatctcgag atcctcgccg ccgtcgccca caagatcgaa
     1201 caggtggcca cgcgccagcc ggtgttccag ctctggtggg tgaccggtgc gctgatcgag
     1261 gcgctgcgcg agcgcggtgt cgagggcggc gtatcgatca agcgcctgct gggccttgcc
     1321 gaccgtgaga tcaagcgcct ctatgaagaa ggcgaggcgc gctactgcca gagcccgccg
     1381 gtcgagctgc tgaacaacat cctctattac atcgcgcgtg ccgaatcgtc gggcccgaag
     1441 gtctcggccg tgcgcgcgtc tttccgtctg ggcgacctgc tgccggtcga cgaggccgtc
     1501 gagcaggaac gcgagagcct gtccgcgccc agcgtgaagc tgatgcgcac ggtgggcgcg
     1561 gcgatccgcg aagaccttgc caaggtcaag gacgtgctcg acatcttcgt gcgccgcggc
     1621 ggcggccagg ccgcggaact cgcgccgcag gtggaactgc tgcgcaagat cggcgatacg
     1681 ctcggcgtgc tgggcctcgg cgagctgcgc acgcgtgtgc agggcgagat cggccggctc
     1741 gaggcgatcg tgtccggcca ggcgcaggtc gatgacggca cgctgctcga gatcgccgct
     1801 acgctgatcg gtatcgagga tcacctcgac gaccaactcg tcggcatgat cgtgcccaag
     1861 gcccgcgatg cgtcccaggg cgccgacgac caggatttcc accaggtgca ggccgccgtg
     1921 ctgcgcgagt gcatgctgaa cctcgcgcgc gtcaaggaag cggtcgcgca gagcgtgggc
     1981 ggcaccctcg gtacggccgg cctcgacagc tggagcgagc tgatgcgcgg catcaaggcc
     2041 ggcctcgtca tgctcggcaa gatgcgcgcg gtcgagatcg tcgacggcat cgggaaccac
     2101 ctgcagcgcg tgatgcgccc cggcgtgccg gcactgcctt cgcacctgct cgaccggctc
     2161 gccgatgcga tcgtgagcgt cgagtactac atggaaacgc tgcaggcccg ccgcagcgag
     2221 ccgttctaca tgctcgataa cgcgcaggcc tgtctcgacg cgctggcggc ggagccggaa
     2281 ccggcggtcc ccaccgtgcc gccgctcggc gaaaccactt ttgcgcgcac gctgcgtatc
     2341 gcacctccca ccacggcgag cggcgcctat cgcgtcgtgc atggcaccga cggcggagat
     2401 ttcgatgcga ccgatgcacc ggccgccagc ccgccgacgc tcgcgccggt gcccgtcacg
     2461 ccgccgacct cggcgctcgc cgatccggag ctcgtcggcc tcttcatcga ggaagccggg
     2521 gaagaggtcg cacgcatcca gcgtgagttc ccgcgctggg aggaaaatcc gctcgaggat
     2581 ggtgcactgt cgatcgtgcg ccgctcgttc cacacgctca agggcagcgg ccgcatggtc
     2641 ggcgcgcgcg agatggccga gttcgcctgg gcgatcgaga atctgctcaa ccgcctcatc
     2701 gacaggacgc tgggccgctc gccggagatc ctcgccaccc tgcgcgacgg cgtgcgggcg
     2761 ctgccgcagt tgctggtcgc gcttgagagc ggctcggcct gcgcactcga cacggcgccg
     2821 atcattgcgc gcgcccacgc cctggcggca caccaggaca cggacgccgc gcgcggcgag
     2881 ggcggcgaga cgacttccgc cgtgcaggtg ccggagaccg cactcaaggc cgccgcgccg
     2941 gtacccgacg ccgatcccgg actgcaggag atcttcaccc gcgagaccgc agcccacatc
     3001 gccacggtgc ggtcttttct cggcgaggag tcacatcgac ccgaaccgca cgtcctgacc
     3061 gaagacgtat accgtgcctg ccatacgctg agcggcagct cgaaggcggc cgtcgcgcgc
     3121 cacggtatcc gtctcgccgg accgctcgat cactggatgc gcaagtcctt cgacagcggt
     3181 gtgggcctca cgtccgcgga cctcgcgctg gtcggcgact gcatgcagga aatggagcgg
     3241 gtggcgggcc atctcgacga ggggacgggc ttcttccaga cgcacgatca gctgcgcgcg
     3301 cgcatcgcgc gcgccgagac ggatctcgac aaacgcattg ccgaggcggc cgaggccgcc
     3361 gaggcggaac aggcggcgag cctgccaccg caggcgccgc cggtgtccgc gcagccgccg
     3421 ggacctcccg agccggccgt gtcggccgac tacgatcccg agatcgcggg catctttgcc
     3481 gaagaggcgg gtgagctgat cgagagcgcc gagcgtgcgc tgcagtcctg gggtgcggat
     3541 cgcgcgagcg gcgcgcatct cgatgccctg aagcggccgc tgcacactct gaagggtggc
     3601 gcgcgcatgg cgggcgtcat cgcgatgggc gatctcgccc acgagatgga aaccctcgtc
     3661 aaccagatgg agtcgggcac ggtgcgggcc gacgaatcgg cccaggccgt gctggtcgcg
     3721 agcctcgacg agatcgcgca tatgcgcgac gcgatcatcg ccggtcgcgg cgtcgcacgg
     3781 gccgatgcgc tgatcgcacg cattcacgca ctcgatccgt cgcgagcggc cgccgcgcct
     3841 gcagcacccg ctgctgcgcc ggctgccgca gcggcggctg tgccggccgt tgccgcagca
     3901 cccgagccgg agccgcgggc cgagcccgaa gaaatcgaaa tcgaactgcc ctcgtcgctc
     3961 gagggcgatg cgggcatcga aaccccggtg gacctgtcgc cgccggccat gccgatcgag
     4021 ccgccggcac ccatcgcacc cgtggcgctc gctgccccgg cagcggtggc cgcgcctgcc
     4081 gcgccgaccg caccggtcgt gatgactcca gccatcgcgc cgccgccgac attcgggccg
     4141 cccactgcgg cgggaaacgg tgagccgctc gcgccgcccg cgccggtgcc gcccggacgc
     4201 gaacctgctg cgcgcggcga gcgccaggaa atggcgcgcg tcgatgccga cctgctcgac
     4261 aggctgctca attactcggg cgaagtcagc atcggccgcg cgcgtctcga acagcagatc
     4321 gcctcgatcg atttcaacct cgccgagctg tcgcgcaccg tcacgcgcct gaaggagcag
     4381 ctgcgcaagc tcgagatcga gaccgaatcg cagatcctgc acacgcacga ggacgaggcc
     4441 ggtcatcgcg ccgagttcga tccgctcgaa ctcgatcgct attcgacgat ccagcaatac
     4501 tcgcgcggcc tcgccgagac cgcgagcgac gtggcgagca tccagcagct gctcgaatcg
     4561 ctcacccagg acacgcagaa tctgctgcag caccaggcgc gcacgatcac cgagctgcag
     4621 aatgggctga tgcgcacccg catggtgccg ttcgagcgac acgtgcagcg cctcgcgcgc
     4681 atcgtgcgcc aggcggcctt cgacacgcgc aagcgcgccg agctgttggt cgagggtgcc
     4741 gccggcgaac tcgaccggca ggtgctcgag cgcatgctgc cggccttcga gcacatcctg
     4801 cgcaactgcg tggcgcacgg catcgaatcg cccgacgagc gcgaggcgcg ctccaagcct
     4861 gcgaccggtc gcattacgct ggcgctgcgc cgcgaaggtg ccgaagtcat cgtgcaggtg
     4921 agcgacgacg gcgccggcat gaatctcaag gcgatccgcg agaagggcca ggcgctcggc
     4981 ctgatccgcc ccgggcaggt gctgtccgat gaggacgtca tgcagctcgt gctcgagccg
     5041 ggcttctcca cggcgggcaa ggtcacgcag agcgccggcc gcggcgtcgg catggatgtc
     5101 gtcgccgaca ccgtcaagaa gctcggcggc gcgctgcaca tggagacgaa ggagggcgag
     5161 ggcacttcgt tcacgatccg cctgccgttc acgctcgcga tcagccatgc gctcgtggtt
     5221 cgcgccggcg acgagttcta cgcgctgccg ctcacgaccg tcgaaggcgt ggtgcgcctg
     5281 acgaaggatg aagtgaccgc gcacctcggg cccgaggcgc cgcagttcga gcacaacggc
     5341 cagcgcttcc gcttccagca cctcgcgcag tacgtgggcc tcgagccctc ggtgctgccg
     5401 gagcaggacg tgacgattcc ggtgatcctc gtgcgcgccg gcgaacactc gaccggtctc
     5461 gtgaccgacg aactggtcgg cagccgggag atcgtcgtca agtccgtggg cccgcagatt
     5521 tcggcgatcc gcggcatttc gggcgccacg atcctcggcg acgggcgcat cgtcgtgatt
     5581 ctcgacatca atgcgctggt gcgcgccgaa tggcgcggac gcagcgagcc ggtcgtgccg
     5641 cgcgaccgca gcgacaagcg cacgctggcg ctggtggtcg acgactcgat cactgtgcgg
     5701 cgtgtcaccc agcgcctgct cgagcgcaac ggcatgcgcg tgctgaccgc gcgcgacggc
     5761 gtcgatgcga tcgaggtgtt gcaggagaac cagcccgaca tcatcctgct cgacatcgag
     5821 atgccccgca tggacggcta cgaggtcgcc gcgcacgtgc gcgccgacga gcgtctgaag
     5881 gagatcccga tcatcatgat cacctcgcgt gtcggcgaga agcaccgtgc gcgcgcgatc
     5941 gagatcggcg tcgacgacta ccttggcaag ccctatcagg aagcgcagct gctcgaggcg
     6001 atcgagccgc tcgtctcgcg ccgccgggag ccggagcggg cgtcgggctc cggcaggggg
     6061 cactgaacca tggccgagcg cgtcagcgag atctacagcc tgctcattcc gctcgtcgac
     6121 gggcgcctga tcgtgccgcg cgcctgcgtg gccgaagtgg tgggctttca gccgccggcg
     6181 gaaatgaccg gcgcgccgcc gtggtatctc ggcaccgtgc cctggaacgg ccgcaccgtg
     6241 ccgctcatct gtttcgaggg cacctgtggg cagggtattc cgcccgccac gggccgcagc
     6301 cgcatcgtgg tgttccactg cctcggctcc cgcgtcgagg gcggctactt cggactggtt
     6361 tcgcagggct tcccgcaact cgtgcgggtg agctccgatg tcgtgcggcc cgacaacacc
     6421 cgctcgttcc cggaacgcca cccggtgatc tgccaggtgc gcatggtcaa cgaagcgccg
     6481 ctcgtccccg acatcgagat gctcgaggaa atgatcgccg acgagacctc cgtcgcggcc
     6541 tgaacggtgc cgcgtctgcg cttgtccggt tattgacggc gcggtgcgcc tgaacgaccg
     6601 cgcaacggca aacccaattc acccgagctc gcgatcgagc gccttgatgc gctcgaggat
     6661 gtcgccgaag cggcgcccga gcgtggtcag ccggtattcg acgtgcggcg gcacctcggc
     6721 gaagacctgg cgctcgatca ggccgaagcg cagcagcttg cgcagccgct cattgagtac
     6781 cttcgcggtg aggcccggga cctggcgctg catcgcgccg gggcgcgcca ctccctggcc
     6841 cacgagatcg agcaccacca gcgaccattt gcagccgacg atgtcctcga ccatgcgggc
     6901 ggcgcgcggc gggcccgcga cggattttct tttcctgagc gtaaccatca atttgcaccg
     6961 gaaagtaagc aaggcaccgt tgcgtgcgga cttacacgcg ggcgggcgcg tgcctaaatt
     7021 gtcgcgtacc gggccctcga ggccaaatcg caggagacca tacagatgaa tgtacgccac
     7081 ctcgcggccg cggccgccct tgccggagtc atcgccgccg gcgccgtcgt cggtgccgag
     7141 gacgatgcgg tgccttatcc ggacggctat cgtgcctggc agcacgtgaa gagcatggtg
     7201 atcggcgagg gtcatccgct gtacggctcg ttcggcggta tccatcatct ctatgccaac
     7261 gagcgggcga tcgccggcta caccggcggg cactttcccg acggcgctgt gatcgtgttc
     7321 gatctgctcg aagcggttgc cgccgatggg gcgatcaccg aaggtcagcg caaggtcgtc
     7381 ggcgtgatgc accgggacgc gaagaagtac gcggccacgg gcggctgggg cttcgaagga
     7441 ttcgcgggcg acagccgttc cgaacgcgtg gtcggcgcgg gcaatggcgc gggctgcttc
     7501 ggctgccacg ctccgcagaa gtcgcaggag ttcgtgttca gcagctatcg gccctgatca
     7561 cgacctccga ggtcgccggc cctcgcctgc agcaccgcca gggcggcgat cgccgcggtc
     7621 tccgtgcgca gcacacgcgg accgaagcgc gccgcgcgcc agccctggcg tgctgccgtg
     7681 acgatctcgt cgtcctcgag cccgccttcg ggcccgatca gcagcgcgag cggcgcggcg
     7741 gtcagttcca tggcaccgag gtcctgttcc gccgacggcg cgagcagcag gcgctggccg
     7801 atgtcggcgg cgagcgcgag tgcggcagcg aacgccatcg gcgcgaggat ctccggcacg
     7861 cggttgcgcc cgcactgctc gcatgcgctg accgccacgg cctgccagtg tgcgagcttg
     7921 cggtcggcgc gctcgccatc cgccacgcgc atgacgctgc gcccgagcgc caccggcacg
     7981 atgcgcgcga tgccgagttc ggtcgccttc tgcacgacca ggtccatttt ctcgccgcgc
     8041 gcgagcccct gcagcaacgt gatcgcgagc ggagattcgc gctcgattcc gcggtgatcg
     8101 ccggtcgcga cgaccgcggc acccttgccg gcgcccacga ggcgcgcggg atactcgccg
     8161 ccgcgcccgt cgaacagcac cagcgggtcg ccggcgtgca gccgcaggac tcgcagcacg
     8221 tggtcgctgg cactgcgcga cagcgtgtgc tcgcgattcg ccgcgagcgg ctcggcggag
     8281 taaacgcgaa caaggcgcat gccgcgattg tggcatgatc gatccatgct gctcgacatc
     8341 gacgccgcca ccggcctcct caggggtgtg cgacaggtgc tgtcaccgtt cttcgaccca
     8401 cgacctgcag ggatgactcc cgacctcatc gtcgtgcaca acatcagcct gccgccgggc
     8461 gagttcggcg ggccgtggat cgacgcgctg ttcacgggcg gccttccgcc gggccgccac
     8521 ccctacttcg agacgctgaa gggtgtgcgc gtttccgccc atgcgctgat ccggcgcgat
     8581 ggcagcgtga cccagtatgt gcccttcgcg cagcgcgcct ggcatgcggg caagtcggtg
     8641 taccacgggc gcaccgactg caacgatttc tcgatcggta tcgagctcga aggcacggac
     8701 gacgtgccgt tcgagggcgc gcagtacacg acgctggccg acctgatccg cgcgctgctc
     8761 gcgatctatc ccacgctcac gcgtgaaagc atcacgggcc acagcgatat tgcgccggga
     8821 cgcaagacgg atcccggccc ggccttcgac tggcccagac tgcgcgcgct gatcgcgtga
     8881 gcggatcgct tcagcggccg cgatcgtggc gccacagcac ctcggtgccg tgctccgcgc
     8941 gggccagcac gcgcgcgatc acgaacagca ggtccgagag gcgattgagg tagcgcggca
     9001 cctcgggcgc cacatcgctc gcgctggcga gggtccacac ctcccgctcg gcacggcgac
     9061 acaccgcgcg cgccacatgg cacgcggccg ccgccaggcc accgcccggc aggatgaatt
     9121 ccttgagccg cggcaggtcc tcgttgaacc ggtcgagatc gtcttcgagg cgcaccgtgt
     9181 gctcgccggt gatcacccgc gcgccgggga tgcacagctc gccgcccagg tcgaacaggt
     9241 cgtgctgcac ctcggtgagg cagcgccgga tggtctcggg cacgcccgcg tgcgccagca
     9301 cggcaccgat ggcgctgttg gtctcgtcca ccgtgccgta ggcggcgacg cgcggatcgt
     9361 ccttcgagac acgcgtgccg tcgccaagcc cggtggtgcc gtcatcgccg gtgcgggtgt
     9421 agatcttcga gagccggttg cccatgacgc caacgctact tgaacgtgaa gcgcgtcgcc
     9481 agcgtgaggc tccggccagc ggtgttgtag ccgttcacca gcgtgtagcc ctcgtcgaac
     9541 gcgttggcga gccgggcctg caccgaccag ctgtcgctca gcgcgaaccg tgcgctcgcg
     9601 ttcacgagca catagctgtc gagcgtgacc gtggcgggaa aggccgtgtc gtggcggtcc
     9661 ccggcatggc tcacgtcgac cccgaagctg aaacgcgcgc ccacgcgttc gagcgcgagc
     9721 acgtagttct cccgcgcgcg gcgcagcagg cgctcgcccg tgtcccggtt gcgcgggtcc
     9781 tgcagcgtgg cctcggcgcg cgcctgccat ttctcgccgc ggaacgcgta gccgagctcg
     9841 acaccttcga tccgggcccg ctcgacgttc tcgttgcggc catcgaaggt caccggatcg
     9901 aacacgaaca cgaccagggc gtcgatgtcg ttgcggaagg cgttggcgta gaggctgtgc
     9961 cgggtgccga tcgcctgccg caggccgatt tcgatctgct cggaaacctc gggctcgagg
    10021 tccgggttgc cgccgaagcc gtagcggtcg gtactgtccg gtgcacggaa cgcgcggccc
    10081 gcggccagcg tgacgcgcgt cgccgtgggc agcagaaagc cgtactcggc gttccagacc
    10141 agctcctcgc cgaaggtctc gtgatcgacg aggccgactg cggcgacgag gctctgccgg
    10201 ccgaacaccg cgcggtcctg cacgaagaac tggttgacgt cggtgccgat gtcgaatgcc
    10261 gtgccgtacg acagcgcttc ggtatcctcg cgcgtcagca gcgcgccgag cgtcagctca
    10321 ttggtgtcgc cgaggcggat gtcgccctgc agttcgaggc catcgcgcgc ggtacgcagg
    10381 aagtcgtagg gcggcgcagc gacgccggcg ttgttctgtt gctgctcgat ctcgtcttcg
    10441 atgcgggtca gcgttgcgcg cacgtcgaca ttgctgcggg ctcgccacag cgactcgatc
    10501 gcatagctcg cgttctcgaa gtcctggtcg accgccgcga ggctcgacgc gaatgtgacc
    10561 ggatcgaaca cggtggtcgt gtaggcggaa ttgcccgcgg cgcgccacgc acgcgcgcgc
    10621 aggtcgagcg tggccgcggg ggagtactcg gccgcgaggt tcacggtcag gttctcgaag
    10681 ccgcggtcgg tcgtgtcacc gaccagcgtg ggaaagcctg cgctctcccg gtaatcgacg
    10741 ccgaagccga gtccggcgga ttgcccgatc cgccagccgc cggcgaggcc accgttcacg
    10801 gtctcgtaac ggccgccgga caggtacccg gtgaggccac gctgcgcggc cgcgcgtgtg
    10861 cgcacgttca cgacgccgcc gatggcgtcg gacccgtaca gcgtcgagcg tggtcccttg
    10921 accacctcga tgcgctgcac gctctcgggc gcgatgttct gcagcgccgc gctgccgatc
    10981 gtaccgggat tcacgcgtac gccgtcgatc agcacgacgg tgtgattgct gtccgtgccg
    11041 cgggtgaaca tcgatgtctg ctggccgggt ccgccggtgc gcgcgagctc gatgcccgcg
    11101 tgcgcgcgca gcaactcgcc cgcatcaggt gcgagggaac gctcgatctc ctcgcgatcg
    11161 atgacgaaca gcggcgcgat cagctcatcg agcgcgacgg cggtacgcgt cgcggtgacc
    11221 acggtctcat cgagggccgg atccgcggcc agggcaaggg tggagggggc cgtcgcgacg
    11281 gcgacggaca cgatgacggc gacctgcgcc gcacggaata cgagagacac tgcaaaatcc
    11341 tttccccatg cacgcccgca tggttggcat gaaacgctgg aacccgtgcc aggccggtct
    11401 ccggacttgc aggcaaggtg cccgggcgta aaccctggcg cctcctcgca tcaccttccc
    11461 gcgcgcctgc gcagtggcgt ctccccgggg ggagaccgat gcgattgatg cctgcctacc
    11521 gttgcggggg cagtgccgga atggcgcgtc gcgcttcacc ggcttcccgt acaccttggc
    11581 agcgggagaa ctttatcaca tacactgcgc ctcccatgtc actcgcccat gaactcgccg
    11641 cagcgcaaga ggcggcccgc gccgctgccg atgtcatcaa tgcgctgtat cggcgcaacc
    11701 tcgcgatcac ggtgaaggcg gaccgctcgc cggtcaccga ggcggacgtg cgtgccgagg
    11761 aggcgatccg tgaagtgctc acgcggcgct ttccggcata cggcttctac ggcgaggaga
    11821 ccggccagag cgcgatgggc gccgagagca tctggctcgt cgatccgatc gacggcacca
    11881 agtccttcat ccgcgactgc ccgttcttct cgacgcagat cgcgctgctg cgcgccggcc
    11941 agctggtgct cggcgtctcg agcgccccgg cctacggcga actggcctgg gcggcgctgg
    12001 gcgagggcgc cttcctcgac gggcagccgg tccgcgtcag cgcgaccggc gcactcgcgg
    12061 acgccatcgt ctcgaccggc aacctgaaga gcctcgcgcg ctcaccgcgg ggctgggcag
    12121 gctacggcca gctcgtcacg caggtgagcc gcatccgcgg ttacggcgac ttcgtgcact
    12181 accatctgct cgcgcgcggc gcgctcgatc tcgtgatcga gtcggacgtg aatatcctcg
    12241 acatcgccgc actcacggtg atcgtgcgcg aggcgggcgg ggtgttcacc gatctcgagg
    12301 gcggcgaagt gggtcttgcg acgacctcgg tgctggccgg caatgccgcg ctgcacgccg
    12361 ctgtcctgcc gctgttcgcc tgagccgctc aggccatcac gaacacgcgc cggccttcga
    12421 cctccgcggt gagcatcggc gcgagataga gcgccgagag attctcggtc gtgagcacgt
    12481 cgggcgcttc gccggcgagc cagcgaccat cgccatagag cagcacgaca cgatcggcgt
    12541 agcgtgccgc gagggtcggg tcgtgcagcg tggcgaccac ggcgcacccg ccatgcgtct
    12601 gctcccgaaa gagatcgagc accgcgagct ggtggtgcgg gtcgaggtgg ttggtcggct
    12661 catcgagcag gaacagcccg ggctgctgcg cgagcagtgc ggcgatcgcg acgcggcgct
    12721 gctcgccgcc cgagagggtg gccatcttgc gcggtccggc gaggtcgagg cccacgcaag
    12781 atg
//
LOCUS       DAOQVY010000065        12779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_5534_length_12779_cov_5.498821, whole genome shotgun sequence.
ACCESSION   DAOQVY010000065 DAOQVY010000000
VERSION     DAOQVY010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12779
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5534_length_12779_cov_5.498821"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /gene="cysS"
                     /locus_tag="PKN91_07445"
     CDS             complement(<1..1022)
                     /gene="cysS"
                     /locus_tag="PKN91_07445"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNS27942.1"
                     /translation="MLRIHNSLTGRKEPLQPLEPGKIGMYVCGITVYDYCHVGHARSQ
                     VAFDVVRRWLRESGFDVTYVRNITDIDDKIIQRAAERGETINELTARFIAAMDEDFGA
                     LGIERPDHEPRATGYLPAILRMIEALLDRGYAYIAADGDVLYAVEKFEGYGRLSGKRL
                     ADLEAGARVDVDAGKRNPLDFVLWKAAKPGEPAWDSPWGAGRPGWHIECSAMSTEILG
                     TRFDIHGGGMDLKFPHHENEIAQSCAATGDEFARVWLHNGFVNVDDEKMSKSLGNFFT
                     LREVLPTVRHPEVMRYFLISSHYRGPINYSTAQLEQADAALTRLYTALRGLSPTPASR
                     PHSAAR"
     gene            complement(1025..2434)
                     /gene="gltX"
                     /locus_tag="PKN91_07450"
     CDS             complement(1025..2434)
                     /gene="gltX"
                     /locus_tag="PKN91_07450"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNS27943.1"
                     /translation="MPDPSLVTRFAPSPTGALHLGNARTALFNFLLARHGGGEFILRI
                     EDTDAERSSDAYLAALIGDLRWLGLEWQAGPDIGGHAAPYRQSERTGLYADFQERLLV
                     TGAAYPCYCTPAELELSRRAMLAAGKAPRYAGTCRDLTAAERRDREAHGLASTLRYRV
                     RPGAEVSFDDIVHGTQRFATDDIGDFIVRRADGSAAFFFCNAVDDALMGVTHVLRGAD
                     HLANTPRQVLVLTALGLPVPTYGHVSLIVGDDGAPLSKRHGATSLAELRERGYLAAAV
                     RNHLFRLGHAPAVQGLLDIDAMAAAFDLRHLGRSPSRFDMVQLDVWQKDAVHALSAAA
                     FADWAAAALPAGLAPAELESFIAVVRPNVVFPADVRAWAEVVYGEPPALTGNGEAMVR
                     EAGAAFFEAAAAAAAASDNDLAAITAAAREASGRKGPALYKPLRLALTGLDHGPELAQ
                     LLRAMPRAKARERLIRYAH"
     gene            2475..3068
                     /locus_tag="PKN91_07455"
     CDS             2475..3068
                     /locus_tag="PKN91_07455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004927153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNS27944.1"
                     /translation="MSSPLRKFALIAAALAMSASSQAQSADAPRVRVTTSLGAFVIEL
                     NPDRAPLTVANFLRYVSEGYYTNTLFHRVVANFVIQGGGITPEYEAKSPHDPIPNEAG
                     NGLQNLRGAVGLARAAGPHTGDAQFFVNVGNNADLDPLPTRWGYAVFGKVVEGMDVVD
                     RISVQPTGAVGGLKADAPLKPVIIEGVTLIGGAPPAQ"
     gene            3065..3787
                     /locus_tag="PKN91_07460"
     CDS             3065..3787
                     /locus_tag="PKN91_07460"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNS27945.1"
                     /translation="MTHLFVSDLHLDAAAPEASDQFIGWLRGEARLATALYILGDLFE
                     SWVGDDDDDAERARVCEALRDYTDGGVPCFVMHGNRDFLLGERFAAHTGCRLIADPVI
                     AVVHGVRTLLAHGDLFCTDDVKYQQLRTIVRSPVWQRRLLAMSLADRKLLADEARAGS
                     RAHTSRVVPEIMDANPAAIEKLVRHLNVDVVIHGHTHRPGVHDFNVDGRPRTRIVLGA
                     WYEQGSVLEWSVGGQALRTLAR"
     gene            complement(3784..4386)
                     /locus_tag="PKN91_07465"
     CDS             complement(3784..4386)
                     /locus_tag="PKN91_07465"
                     /inference="COORDINATES: protein motif:HMM:NF017939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HNS27946.1"
                     /translation="MSAWFLAAPTPQAWVDAAAVNWRMLLNDHANCEKKAASTALALM
                     FAYPEDVALSVALARLAREELRHFEQVVRLMQKLEVPFERLTPSRYAAGLRRHASSAE
                     PRRKLDLMLIGALIEARSCERFRLLAPRLPPQVAEFYADLERAEARHHELYIEHAKAS
                     AGEADWQLRLRQLAQVEAGLVTSPDKLLRFHSGPVPDLRA"
     gene            complement(4383..5627)
                     /locus_tag="PKN91_07470"
     CDS             complement(4383..5627)
                     /locus_tag="PKN91_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNS27947.1"
                     /translation="MRLLMIDHDPRYRALVRHHVTCRWPEAEVVTYNPARRGPLAPEF
                     LAQGFDAVVLDHSSPAGAGYGWLEELAARPGFAPIIFFAPDDELATRERAIEAGAFDT
                     FARNQIDNARLLDALERAATAHLDALSVWRGSPEGLDAQYFGRAQLRGYRRVRELARG
                     SVSDLFLAEGEEAGELVVLKVTHALRKANGVDQSFDRFLQEYEIVRRLRHPNVVRIHD
                     LGVMDEFAFIVMEYFAGGDLRRRMRAPLPLREAFAYALEIARALAAVHGAGVRHRDLK
                     PGNVMVRDDGSLALIDFGLAKHEALAFEITDTGMIFGTPHYMSPEQGHGQATDERSDL
                     YSLGVMLYEMLTRTKPFDADNPMAIIYLHAKAPVPRLPPPLARAQPIIDRLMAKDRDA
                     RFASADEVAAMLEMAIEELGPE"
     gene            complement(5636..6811)
                     /locus_tag="PKN91_07475"
     CDS             complement(5636..6811)
                     /locus_tag="PKN91_07475"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HNS27948.1"
                     /translation="MNAAAQPRERALLLDCFSAALRAVDGRARVHAALTNGTAGLPVD
                     AGRVVVLAVGKAAPRMTLGAIDALGSRVARALVITKSGHVDEGLRHLADVTVIESAHP
                     VPDSRSLAAGAQLLGWVESLAPGEFPLFLVSGGASSLAEVLHANVTLDELAALNRRLL
                     AEGVAIGEINAQRRALSRIKGGALIERLQGRAGCALFMSDVPGDDPAVIGSGLAGPAA
                     RDRPHRIVIANVEDAMAAAAGRAREAGLRVQIGEARFAADAARLGARFAATIATGAAD
                     VFVWGGESTVELPAAPGRGGRNQQLALAAARDLHGRHGLTLLAAGTDGIDGPTTDAGA
                     IVDGGTWQRVRDAGLDGDDCLARADAGAALEAAGDLLHTGPTGANVGDLVIGLACMA"
     gene            complement(6808..8319)
                     /gene="purF"
                     /locus_tag="PKN91_07480"
     CDS             complement(6808..8319)
                     /gene="purF"
                     /locus_tag="PKN91_07480"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNS27949.1"
                     /translation="MCGIVGIVGTSPVNQRLYDALTMLQHRGQDAAGITTSNEGELCV
                     RKGSGLVRDVFQQHHMLELRGNVGIAHVRYPTAGCEGASEAQPFYVNAPYGICLGHNG
                     NLTNAAELAEVLIREDRRHLNTSSDSEVLLNVFASELQRIGGARVAPADIFAALATVY
                     RRCRGGYAAIAMIIGHGVVGFRDPNGIRPLVLGTRDTARGPEWMLASESVALTGLGFR
                     FVRDVGPGEAVFIDERGRLHAQQLVEGARHTPCIFEYVYFARPDSIIDNISVYRSRMR
                     MGDKLAERIRSERPDHDIDVVIPIPDTSRTAAMQVAQLLGIKYREGFNKNRYIGRTFI
                     MPGQEQREKSVRRKLNAIDFEFRGKVVLLVDDSIVRGTTSAQIIELAREAGARKVYFA
                     SAAPPVRFPNVYGIDMPAANELVANNRTVDEVSAAIGADWLVYQSLEDLVQACLHDNA
                     TITEFDTSCFSGEYVTGDVTPEYLERLRTERCDAAKARRRDAERAELRVVRGG"
     gene            complement(8325..8822)
                     /locus_tag="PKN91_07485"
     CDS             complement(8325..8822)
                     /locus_tag="PKN91_07485"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNS27950.1"
                     /translation="MLIGIVVISAIIGLFRGLMREALSLVTWVAALWCAWHFGPGLEP
                     HLGGLLDSSAVRPWAARVIIFFGVLLLGMFAGWLVTQFVRVSLFQSVDRLFGVVFGLL
                     RAFVVIGALAILGQALELDRETWWKKAKLTPYAENAGSGLRSIVGDDPVARAQELLLS
                     TGGRE"
     gene            complement(8920..9528)
                     /locus_tag="PKN91_07490"
     CDS             complement(8920..9528)
                     /locus_tag="PKN91_07490"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNS27951.1"
                     /translation="MNKQFRERLTGAVILVIFVVVVVPALLSGPPQPAAPATTDTTAE
                     GPPLRSYTIDLTDPAAVPARAPASTPASPPAPVREPAADPAPEPAPAATRPAQELVAE
                     PAPAAPAVAPAPEPAPVAAATAEVESGFAVQIGSFANRSNADSLVARLRKGGFPAFIA
                     PTSSGRKLYRVRVGPVADRAEAQILATRLATAGQPGSVVSHP"
     gene            complement(9536..10783)
                     /gene="folC"
                     /locus_tag="PKN91_07495"
     CDS             complement(9536..10783)
                     /gene="folC"
                     /locus_tag="PKN91_07495"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF008101.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HNS27952.1"
                     /translation="MRSLAEWIELQQRVHPRAIDLGLERVARIARALGVERPDAPTWI
                     VGGTNGKGSTIAYLHSILAAAQFTTGVFTSPHLVRYNERVRVGGAEVPDAELIEAFEA
                     IEAARGATTLTFFEFSALAALLVFRWRGVDAQLLEVGLGGRLDATNLVDADVAVLCSV
                     GSDHHQWLGPTLEDVGREKAGIFRHGRPAIIGAADLPASVFTEVERIGAVPHVARRDY
                     DWEHSGERWTYRSRARTLADLPIPALVGEVQFANAAAAIAALDAGPLPVSAHAVARGL
                     EQARLPGRFQVVPGEVEWILDVAHNAPAAAELARNLAARAVRGRTLALAGFLDDKDVA
                     AIGRVLAPHIDAWVIASLPPPRGLTAAQVAGELGIGAPLAQCESMADACAQARAATRS
                     GDRIVAFGSFHLVGPVLEWLQLY"
     gene            complement(10800..11669)
                     /gene="accD"
                     /locus_tag="PKN91_07500"
     CDS             complement(10800..11669)
                     /gene="accD"
                     /locus_tag="PKN91_07500"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNS27953.1"
                     /translation="MSWFEKIMPSRIKTERRTRSVPEGLWIKCPACDSVLYRAELERN
                     LNVCPKCSHHMRIGARERLALFLDEGEVVEIGADITPQDPLKFKDSKRYKDRLASAQK
                     NTGENDALVALAGTLLGIDVVACAFEFRFLGGSMGSVVGERFKRSVDYCIEHRAALVC
                     FSASGGARMQEALYSLLQMAKTSAALARLAREHLPFISVMTDPTTGGVSASLAMLGDL
                     NVAEPRALIGFAGPRVIQQTVRETLPEGFQRSEFLLEHGALDMIIDRRDMRERLGSVL
                     RLLLKKPAAATVA"
     gene            complement(11671..12453)
                     /gene="truA"
                     /locus_tag="PKN91_07505"
     CDS             complement(11671..12453)
                     /gene="truA"
                     /locus_tag="PKN91_07505"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNS27954.1"
                     /translation="MPRVAVGIEYLGTAYCGWQAQAGQRTVQQQVEAALSAVADEPVA
                     VVCAGRTDTGVHALGQVVHFDTAAARAPRAWVLGANTRLPQDISLLWAQEVPSHFHAR
                     YSALSRSYRYVIVNRGQRPALAAGRVAWVHRPLDVASMRAAAAHLLGEHDFSAFRSAD
                     CQSKTPMRRITAIAVERSGERITIDVTANAFLHHMVRNIAGLLIAIGRGDAGPEFAAE
                     VLAGRDRRRNAATAPAGGLYFTAVRYDASFGLPDGVSAMMPP"
     gene            complement(12453..>12779)
                     /locus_tag="PKN91_07510"
     CDS             complement(12453..>12779)
                     /locus_tag="PKN91_07510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HNS27955.1"
                     /translation="PDIDVDFGATDAFGGATTRNGLDLDVGTSSGDADGAFAATLKIG
                     PEDLALPDLEPVTMSEVGTKLDLARAYMDMGDPDGARSILKEVLQEGSVSQKQEAQRL
                     IDTLPG"
BASE COUNT         2068 a         4430 c         4413 g         1868 t
ORIGIN      
        1 tcccgcgcgg cagaatgcgg ccgcgacgcc ggtgtcggcg acaggcctcg cagcgccgta
       61 tagagtcgcg tcagggcggc atccgcctgt tcgagctgcg cagtcgaata attgatcggg
      121 ccgcggtagt ggctcgagat caggaagtag cgcatcacct ccggatgacg cacggtcggc
      181 agcacttcgc gcagcgtgaa gaaattgccg agcgatttcg acatcttctc gtcatcgacg
      241 ttgacgaagc cgttgtgcag ccacacgcgc gcgaactcgt cgccggtcgc ggcgcaggac
      301 tgcgcgatct cgttctcgtg gtgcgggaac ttgagatcca tgccgccacc gtgaatgtcg
      361 aagcgtgtgc cgagaatctc ggtcgacatc gccgagcact cgatgtgcca gcccggccgg
      421 cccgcccccc agggcgagtc ccacgccggc tcgcccggct tcgccgcctt ccacagcacg
      481 aagtcgagcg ggttgcgctt gcctgcatcg acgtcgacgc gtgccccggc ttcgaggtcg
      541 gcgagccgct tgccagacag gcgaccgtat ccctcgaact tctccacggc gtacagcacg
      601 tcgccgtcgg ccgcgatata ggcgtagccc ctgtcgagca gggcttcgat catgcgcagg
      661 atcgccggca ggtaaccggt ggcccgcggc tcgtgatcgg ggcgctcgat gccgagtgcc
      721 ccgaaatcct cgtccatggc ggcgatgaag cgcgcggtca gctcgttgat cgtctccccg
      781 cgttccgcgg cgcgctggat gatcttgtcg tcgatgtcgg tgatgttgcg cacataggtc
      841 acatcgaagc cactttcgcg cagccagcgc cgcacgacgt cgaaggccac ctgcgagcgc
      901 gcatggccca cgtggcagta gtcatacacg gtgatgccgc agacgtacat gccgatcttg
      961 cccggctcga gcggttgcag gggttccttg cggcccgtca gcgaattatg gatgcgcagc
     1021 atggtcagtg agcgtagcgg atgaggcgct cgcgcgcctt cgcgcgcggc atggcgcgca
     1081 gcaattgcgc gagttcgggg ccgtggtcga gcccggtgag tgcgaggcgc agcggcttgt
     1141 agagcgccgg gcccttgcgc ccggaagctt cgcgcgccgc agccgtgatg gccgcgaggt
     1201 cgttgtcgct cgccgctgcg gcggcggccg cggcctcgaa gaaggcagcg cccgcttcgc
     1261 gcaccattgc ctcgccgttg ccggtaagcg ccggcggttc gccgtagacc acctcggccc
     1321 aggcacgcac gtcggccggg aacaccacat tcgggcgcac gaccgcgatg aaggactcga
     1381 gctccgccgg cgcgaggcca gccggcagcg ccgctgcggc ccaatcggcg aacgcggcgg
     1441 ccgacagggc gtgcacggcg tccttctgcc acacgtcgag ttgcaccatg tcgaagcggc
     1501 tcggcgagcg gccgagatgg cgcaggtcga acgcggccgc catcgcatcg atgtcgagca
     1561 ggccctgcac ggcaggcgca tggccgagcc ggaagagatg gttgcgcacg gcggcggcaa
     1621 gatagccccg ttcacgcagt tcggcgaggc tcgtggcgcc gtgccgcttc gagagcggcg
     1681 caccatcgtc gcccacgatc agcgacacgt ggccgtaggt gggtaccggc agccccagcg
     1741 cagtgagcac cagcacctgg cgcggtgtat tggcaagatg gtccgcgccg cgcagcacgt
     1801 gggtcacgcc catcagcgca tcgtcgacgg cattgcagaa gaagaacgcc gccgagccat
     1861 ccgcgcgccg cacgatgaag tcgccgatgt cgtcggtcgc gaagcgctgc gtgccgtgca
     1921 cgatatcgtc gaatgagact tcggcgccgg gcctgacgcg atatcgcagc gtggaggcga
     1981 gaccatgcgc ttcgcgatcg cgccgctcgg cggccgtcag atcgcggcag gtgcccgcat
     2041 agcgcggcgc cttgcccgcc gcgagcatcg cgcggcggga cagctcgagt tccgccggcg
     2101 tgcaatagca cggataggcc gcaccggtga cgagcaggcg ctcctggaaa tcggcataca
     2161 gcccggtccg ctctgactgg cgatacggcg ccgcgtggcc gccgatgtcg ggccccgcct
     2221 gccactcgag cccgagccag cgcaggtcgc cgatcagggc cgctaggtag gcgtcgctcg
     2281 agcgttcggc gtcggtgtcc tctatccgca gaatgaactc gccgccgcca tggcgcgcca
     2341 gcaggaaatt gaacagtgcc gtgcgcgcgt tgccgagatg cagcgccccg gtggggctcg
     2401 gcgcgaaacg ggtgacgagg ctcggatcgg gcataaacgt catcttaagg gattggtaga
     2461 atggaagaat gatcatgtca tcgccactgc gaaaattcgc cctgatcgcc gcggcgctcg
     2521 ccatgagcgc gagcagccag gcccaatccg ccgatgcccc gcgagtacgc gtcaccacct
     2581 cgctcggcgc cttcgtgatc gaactgaacc ccgatcgcgc gccgctcacg gtcgcgaact
     2641 tcctgcgcta cgtgagcgag ggctactaca cgaacaccct cttccaccgc gtggttgcca
     2701 acttcgtgat ccagggcggc ggtatcacgc cggagtacga ggccaagtcc ccgcacgatc
     2761 cgatcccgaa cgaagccggc aatggcctgc agaacctgcg cggtgcggtc gggctggcgc
     2821 gcgccgccgg gccccatacc ggtgacgcgc agttcttcgt caacgtgggc aacaatgccg
     2881 acctcgatcc gctgccgaca cgctggggct acgcagtgtt cggcaaggta gtcgagggca
     2941 tggacgtcgt cgatcgcatc tccgtgcagc cgaccggcgc cgtcgggggt ctcaaggccg
     3001 atgcgccgct gaagccggtg atcatcgagg gcgtcacgct gatcggcggc gcgccgccag
     3061 cccagtgacc cacctcttcg tctcggacct gcatctcgat gcggcggcgc ccgaggcgag
     3121 cgaccagttc atcggctggc tgcgcggcga ggcccgcctt gcgacggcgc tctacatcct
     3181 cggcgacctg ttcgagtcct gggtcggcga cgacgacgac gatgccgagc gcgcgcgcgt
     3241 ctgcgaggcg ctgcgcgact acacggacgg tggcgtgccg tgcttcgtca tgcacggcaa
     3301 ccgcgacttc ctgctcggtg agcgcttcgc cgcgcatacg ggctgccgcc tgatcgccga
     3361 tccggtcatc gccgtggtcc acggcgtacg caccctgctc gcgcacggcg acctgttctg
     3421 caccgatgac gtgaagtacc agcagctgcg cacgatcgtg cgcagccccg tgtggcagcg
     3481 gcgcctgctt gcgatgtcgc tcgccgatcg caagctgctg gccgacgagg cccgcgccgg
     3541 cagtcgcgcc cacacgagcc gcgtcgtgcc cgagatcatg gatgcgaacc cggccgccat
     3601 cgaaaagctc gtgcgccatc tcaatgtcga cgtcgtgatc cacggccaca cgcaccgccc
     3661 cggggtgcat gacttcaatg tcgacggacg gccgcgaacc cggatcgtgc tcggcgcgtg
     3721 gtatgagcag gggagcgtgc tcgaatggag cgtgggcggg caggcgctgc gcaccctcgc
     3781 gcgctaggcg cggaggtccg gcaccgggcc ggaatggaag cgcagcaact tgtcgggcga
     3841 cgtgaccagc cccgcctcca cttgcgcgag ctggcgcagt cgcagttgcc agtcggcctc
     3901 accggcactg gctttcgcgt gctcgatata cagctcgtgg tggcgcgcct cggcccgctc
     3961 gaggtcggca tagaactcgg cgacctgcgg cggcaggcgc ggcgcgagca gccggaagcg
     4021 ctcgcaggag cgcgcctcga tcagcgcacc gatcagcatc aggtcgagct tgcggcgcgg
     4081 ctctgcgctc gacgcgtggc ggcgaagccc cgccgcgtag cgactgggcg tcagccgctc
     4141 gaacggcacc tcgagcttct gcatgagccg taccacctgc tcgaaatgac gcaattcctc
     4201 gcgcgcaagc ctcgcgagcg cgaccgagag cgcgacgtcc tccggatacg cgaacatcag
     4261 cgcgagcgcc gtggacgcag ccttcttctc gcaattggcg tggtcgttca gcagcattcg
     4321 ccagttcacc gcggccgcat cgacccaggc ctgcggcgtc ggcgcagcga ggaaccacgc
     4381 gctcattcag gccccagttc ctcgatcgcc atctcgagca tcgccgcgac ctcgtcggcg
     4441 ctggcgaaac gcgcgtcgcg atccttcgcc atcagcctat cgatgatcgg ctgcgcacga
     4501 gcgagcggag gcggcaggcg cggcaccggc gccttggcgt gcaggtagat gatcgccatc
     4561 ggattgtcgg catcgaaggg cttggtccgc gtcagcatct cgtagagcat gacgccgagg
     4621 ctgtagaggt cgctgcgctc gtcggtcgcc tggccatggc cctgctcggg actcatgtag
     4681 tgcggcgtgc cgaagatcat gccggtgtcg gtgatctcga aggcgagcgc ctcgtgcttg
     4741 gcgaggccga agtcgatcag cgccaggctg ccgtcgtcgc gcaccatcac gttgccgggc
     4801 ttcaggtcgc gatggcgcac gccggcgccg tgcacggcgg ccagcgcgcg cgcgatctcg
     4861 agcgcataag cgaacgcctc gcgcagcggc agcggcgcgc gcattcgccg ccgcaggtcg
     4921 ccgcccgcaa agtactccat gacgatgaac gcgaactcgt ccatgacccc gaggtcatgg
     4981 atgcgcacta cattggggtg gcgcagccgg cggacgatct cgtactcctg cagaaagcgg
     5041 tcaaaggact ggtcgacgcc attcgccttg cgcagcgcat gcgtcacctt gagcacgacc
     5101 agctcaccgg cttcttcgcc ctcggcgagg aacaggtccg acaccgagcc gcgcgcgagt
     5161 tcgcgcacgc gccgatagcc gcgcagctgc gccctgccga agtactgcgc gtcgagcccc
     5221 tccggcgatc cgcgccagac agacaacgca tcgagatgcg cggtggccgc gcgctccagg
     5281 gcatcgagca gccgcgcgtt gtcgatctgg ttgcgggcga aggtgtcgaa ggcgcccgcc
     5341 tcgatcgcgc gctcgcgcgt cgcgagctcg tcatcgggcg cgaagaaaat gatcggtgcg
     5401 aaacccggcc gtgccgccag ttcctcgagc cagccgtagc cggcaccggc cggcgagctg
     5461 tggtcgagca ccacggcatc gaagccctga gccaggaact cgggcgcgag cggaccgcgg
     5521 cgtgccggat tgtaggtgac cacttcggcc tcgggccagc ggcaggtcac gtggtgccgc
     5581 acgagcgctc gataacgcgg atcgtggtcg atcatcaaca ggcgcatcgt gcggattagg
     5641 ccatgcacgc gagaccgatg acgaggtctc cgacattggc gccggtgggg ccggtgtgca
     5701 gcagatcgcc cgcggcctcc agcgccgcgc ccgcatccgc gcgcgcgagg caatcatcac
     5761 cgtcgaggcc cgcatcccgg acgcgctgcc aggtcccgcc gtcgacgatc gcgccggcat
     5821 cagtggtggg tccgtcgatg ccgtcggtgc cggctgcgag cagcgtgagt ccatgccgcc
     5881 cgtgcagatc acgcgccgcg gcgagtgcga gctgctggtt gcgacctccg cgcccgggcg
     5941 ccgctggcaa ttccacggtc gactctccgc cccacacgaa gacatcggcc gcgccggtgg
     6001 caatcgtcgc ggcgaatcgc gcaccgagcc gggccgcatc ggcagcgaag cgcgcctcgc
     6061 cgatctgcac gcgcaatccg gcttcgcgcg cgcggcccgc cgcagcggcc atcgcatcct
     6121 cgacgttcgc gatgacgatg cgatgcgggc gatcgcgcgc ggcagggccc gcaagccccg
     6181 agccgatcac cgccgggtca tcgccgggca cgtcggacat gaacagcgcg cagcctgccc
     6241 tgccctgcag gcgctcgatc agcgcgccac ccttgatgcg cgacagcgcc cggcgctgcg
     6301 cattgatctc accgatggcg acgccctcgg cgagcaggcg gcggttcagt gcggcgagct
     6361 catccagggt cacgttcgca tgcagtacct cggcgaggct cgacgcgcct cccgagacca
     6421 gaaacagggg gaattcccca ggggccagcg actcgaccca gccgaggagc tgcgcgcccg
     6481 cggccaggct gcgcgagtcg ggaactggat gggccgactc gatgaccgtc acgtccgcca
     6541 ggtgtcgcag accctcgtcg acgtggccgc tcttcgtgat cacgagcgcg cgcgccacgc
     6601 gcgatcccag cgcatcgatt gcgccaagcg tcatgcgcgg ggcggccttg ccgacggcca
     6661 gcaccaccac acgcccggcg tcgaccggca ggcccgcggt gccgttcgtc agtgcggcat
     6721 gcacgcgggc gcggccatcc acggcgcgca gcgcggcact gaaacagtcg aggagcagcg
     6781 cgcgctcgcg tggctgcgca gctgcgttca cccgccccgc acgacccgca gttcggcgcg
     6841 ctcggcatcg cgacggcgcg ccttggcggc atcgcagcgc tcggtgcgca gccgttccag
     6901 gtactcgggc gtgacgtcac ccgtgacgta ctcgcccgag aagcacgacg tatcgaactc
     6961 ggtgatcgtg gcgttgtcgt gcaggcaggc ctgcaccagg tcctcgaggg actggtacac
     7021 gagccagtcg gcaccgatcg ccgccgagac ctcatcgacc gtgcgattat tcgcgacgag
     7081 ttcgttcgcg gccggcatgt cgatgccgta gacgttcggg aagcgcaccg gcggcgccgc
     7141 cgaggcgaaa tacaccttgc gcgcgccggc ctcgcgcgca agctcgatga tctgcgccga
     7201 agtcgtgcca cgcacgatcg aatcgtcgac cagcagcacg accttgccgc ggaactcgaa
     7261 gtcgatcgca ttcagcttgc ggcgaaccga tttctcgcgc tgctcctggc ccggcatgat
     7321 gaacgtgcgg ccaatgtacc ggttcttgtt gaagccctcg cgatacttga tgccgagcag
     7381 ctgggcgacc tgcatcgctg ccgtgcgcga ggtgtcggga atcgggatca cgacgtcgat
     7441 gtcgtggtcg ggtcgctcgc tgcggatgcg ctcggcgagc ttgtcgccca tgcgcatgcg
     7501 tgagcgatag accgagatgt tgtcgatgat cgaatcgggg cgcgcgaaat acacgtactc
     7561 gaaaatgcag ggcgtatggc gcgcgccctc gaccagttgc tgggcgtgca gacgtccgcg
     7621 ctcgtctatg aacacggcct caccgggacc gacgtcgcgc acgaagcgga acccgaggcc
     7681 ggtcagcgcg acgctctccg aggcgagcat ccactccggg cccctggccg tatcgcgcgt
     7741 gccgagaacc agcgggcgaa tgccgttcgg atcgcgaaaa ccgacgacgc catggccgat
     7801 gatcatcgcg atcgccgcat agccgccgcg acagcgccgg tagaccgtgg caagcgctgc
     7861 gaagatatcc gccggcgcga cccgcgcccc gccgatgcgc tgcaactcgg aagcgaatac
     7921 gttcagcagc acttccgagt ccgacgaggt gttgaggtgg cgccggtcct cgcggatcag
     7981 cacttcggca agttcggcgg cattggtcag attgccgttg tggccgaggc agatgccgta
     8041 cggggcgttt acgtagaacg gttgcgcctc ggacgcgccc tcgcagcccg cggtcgggta
     8101 gcgcacgtgc gcaatgccga cattgccacg cagttcgagc atgtggtgct gctggaacac
     8161 gtcgcgcacg agaccgctgc ctttgcgcac gcacagctcg ccttcgttcg aggtggtgat
     8221 gcccgcggcg tcctggccgc gatgctgcag catggtcagc gcgtcgtaga gccgctggtt
     8281 gacggggctc gtgccgacga ttccgacgat gccacacatg cgcgctactc cctgccgccc
     8341 gtggacagca gcaactcctg cgcacgcgcc accggatcgt cgccgacgat gctgcgcagc
     8401 ccgctgccgg cattctcggc atagggcgtg agtttcgcct tcttccacca ggtttcccgg
     8461 tcgagctcga gcgcctggcc gaggatggcg agcgcaccga tcacgacgaa ggcgcgcagc
     8521 aggccgaaca cgaccccgaa cagccggtcg acgctctgga acagcgatac gcgcacgaac
     8581 tgggtcacca gccagccggc gaacatgccg agcagcagga cgccaaaaaa gatgattacg
     8641 cgcgcggccc atggacgcac cgcggaagag tcgagcaggc cgccgaggtg tggctcgagt
     8701 cctggcccga agtgccaggc gcaccacaat gccgccaccc aggtcaccag cgagagcgct
     8761 tcgcgcatca ggccgcgaaa cagcccgatg atcgccgaga tcacgacgat accgatcaac
     8821 acgtagtcaa ttgtcgtcat gagccctggt agccgctcgc gccgcgtctg cgcgcgcgga
     8881 ttgtacaaga tggaggtggg gcgcggaaaa gcaacccggt cacggatggc tgacgaccga
     8941 tccgggctgg cccgccgtgg caagccgcgt tgcgaggatc tgcgcttcgg cacgatcggc
     9001 aaccgggccg acacgcacgc gatacagctt ccgcccgctg ctcgtgggtg caatgaacgc
     9061 gggaaagccg cccttgcgca gccgtgccac cagactgtcg gcattgctgc ggttggcgaa
     9121 actgccgatc tgcactgcaa accccgactc gacttcggcg gtagcggcgg cgaccggtgc
     9181 aggttcgggc gcaggagcca cggcgggcgc cgcgggcgca ggctcggcca ccagctcctg
     9241 cgccggcctg gtcgctgccg gagcgggctc gggcgccgga tccgccgccg gttcgcgcac
     9301 cggagccggc ggcgaggccg gcgtcgacgc gggcgcgcgg gcaggcaccg ccgccgggtc
     9361 ggtcaggtcg atcgtgtagg agcgcaacgg cggcccctct gcagtcgtat ccgtcgtcgc
     9421 gggcgcggcc ggttgcggcg gcccgctgag cagcgcgggc acgacgacga ccacgaaaat
     9481 gacgaggatc acggcccccg tgagccgttc cctgaactgc ttgttcacgc cgaaatcagt
     9541 atagctgaag ccactcgagc accgggccga cgagatggaa ggaaccgaag gcgacgattc
     9601 gatcgcccga gcgcgtcgcc gcgcgcgcct gcgcacaggc atcggccatc gactcgcact
     9661 gcgcgagcgg cgcaccgatg ccgagttcgc cggcgacctg cgcggcggtg agcccgcgcg
     9721 gcggcggcag cgaggcgatc acccacgcgt cgatatgcgg cgcgagcacg cgccctatcg
     9781 ccgcgacatc cttgtcgtcg aggaagccgg caagcgcaag cgtacgtccg cgcaccgcgc
     9841 gcgccgcgag gttgcgcgcg agttccgcgg ccgcaggcgc gttgtgtgcg acatcgagta
     9901 tccactcgac ctcgcccggc acgacctgga agcgaccggg caggcgcgcc tgctcgagcc
     9961 cgcgcgcgac cgcatgggcc gatacgggca gcgggccggc atccagcgcg gcgatcgcgg
    10021 cggccgcatt cgcgaactgc acctcgccga cgagcgcggg tatcggcagg tcggcgagcg
    10081 tgcgggcccg tgaccgatag gtccagcgtt cacccgagtg ttcccagtcg tagtcgcgcc
    10141 gcgcgacatg cgggacggcg ccgatgcgct cgacctcggt gaagacgctc gcgggaaggt
    10201 cggcagcgcc gatgatcgcg gggcgaccgt gccggaagat gccggccttc tcgcgcccga
    10261 catcctcgag cgtgggcccg agccactggt ggtgatcgga tcccacggag cacagcaccg
    10321 ccacatcggc atccaccagg ttggtggcat cgaggcgtcc gccgaggccc acctcgagca
    10381 gctgcgcatc gacgccacgc catcggaaca ccagcagcgc cgcgagcgca ctgaactcga
    10441 aaaaggtcag ggtcgtcgcg ccgcgcgcgg cctcgatcgc ctcgaaggcc tcgatgagtt
    10501 ccgcatcagg cacctcggcg ccgccgacac ggacccgctc gttgtagcgc accaggtgcg
    10561 gggacgtgaa cacgccggtg gtgaactgcg cggcagccag gatcgaatgg agatacgcga
    10621 tcgtcgagcc cttgccgttc gtgccgccga cgatccaggt cggcgcatcg ggccgctcga
    10681 caccgagcgc gcgcgcgatg cgcgcgaccc gctcgagccc gagatcgatg gcgcgcggat
    10741 gcacgcgctg ctgcagctcg atccactcgg cgagcgagcg catggccggt gcgcgccggt
    10801 caggcgacgg tcgccgccgc cggcttcttc agcagcaggc gcagcacgga gccgaggcgc
    10861 tcacgcatgt cccgacggtc gatgatcatg tcgagcgcgc cgtgttcgag caggaactcg
    10921 ctcctctgga agccctcggg cagggtctcc cgcacggtct gctggatgac acgcggaccg
    10981 gcgaagccga tcagtgcccg cggctcggcg acgttcaggt cccccagcat cgccaggctc
    11041 gccgacacgc cacccgtcgt cgggtcggtc atgaccgaga tgaatggcag gtgctcgcgc
    11101 gcgaggcgcg cgagcgcggc actggtcttg gccatctgca gcagtgaata gagtgcctcc
    11161 tgcatgcgcg ccccgccgct tgcggaaaag cacacgagcg ctgcgcgatg ctcgatgcag
    11221 tagtcgaccg aacgcttgaa ccgctcgccg accaccgaac ccatggagcc gccaaggaag
    11281 cggaactcga aggcgcaggc caccacgtcg atgccgagca gcgtgccggc aagcgcgacc
    11341 agcgcgtcgt tctcgccggt gttcttctgc gccgaggcga gccggtcctt gtagcgcttg
    11401 ctgtccttga acttgagcgg gtcctgcggc gtgatgtcgg cgccgatctc gacgacctcg
    11461 ccctcgtcga ggaacagggc cagccgctcg cgcgcaccga tgcgcatgtg gtggctgcac
    11521 ttcgggcaga cattgaggtt gcgctcgagt tcggcgcggt acagcaccga atcgcaggcc
    11581 ggacacttga tccacaagcc ctcggggacc gagcgcgtgc ggcgttcggt cttgatgcgc
    11641 gagggcatga ttttttcgaa ccaggacata tcagggcggc atcatagcgg aaaccccgtc
    11701 cggcaagccg aagctcgcgt catagcgcac ggcggtgaag tacaggccgc cggcgggcgc
    11761 cgtggcggca ttgcgccggc gatcgcggcc cgcgagcact tcggcggcga actccggtcc
    11821 ggcgtcccca cgcccgatgg cgatcagcaa tcccgcgatg ttgcgcacca tgtggtgcag
    11881 gaaggcgttg gcggtgacgt cgatcgtgat gcgctcaccg ctgcgctcga ccgcgatcgc
    11941 ggtaatgcgg cgcatgggtg tcttcgactg gcaatcggcg gagcggaacg cgctgaaatc
    12001 gtgctcaccg agcaggtgcg cggcggcggc gcgcatcgag gcgacatcga gcggccggtg
    12061 cacccaggcg acgcggcccg cggcgagtgc gggccgctgg ccgcgattga cgatgacgta
    12121 gcgataactg cgcgacaggg cgctgtagcg cgcgtggaag tgcgacggca cctcctgcgc
    12181 ccagagcaac gaaatgtcct gcggcagcct ggtattggcg ccgagcaccc aggcgcgcgg
    12241 cgcgcgcgcc gcggcagtgt cgaagtgaac cacctggccg agcgcgtgca cgcccgtgtc
    12301 ggtacggccc gcgcagacga ccgccaccgg ctcatcggcc acggccgaca acgccgcctc
    12361 gacctgctgc tgcacggtac gctggcccgc ctgcgcctgc cagccacagt aagcggtgcc
    12421 gaggtactcg atccctaccg cgacacgcgg catcaacccg gcagggtgtc gatcaggcgc
    12481 tgcgcctcct gcttctgcga gaccgagccc tcctgcagga cttccttcag gatgctgcgg
    12541 gcgccgtcgg gatcacccat gtccatgtag gcacgcgcca gatcgagctt ggtgccgacc
    12601 tcgctcatcg tgacgggctc gaggtccggc agcgcgaggt cttccgggcc gatcttcagg
    12661 gtcgccgcga aggcgccgtc ggcgtcgccc gacgatgtgc cgacgtcgag atcgaggccg
    12721 ttgcgcgtcg tcgcgccgcc gaacgcatcg gtggcgccaa agtcgacgtc gatgtcggg
//
LOCUS       DAOQVY010000066        12719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_5569_length_12719_cov_5.680433, whole genome shotgun sequence.
ACCESSION   DAOQVY010000066 DAOQVY010000000
VERSION     DAOQVY010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12719
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5569_length_12719_cov_5.680433"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="PKN91_07515"
     CDS             <1..1048
                     /locus_tag="PKN91_07515"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNS27956.1"
                     /translation="ARRHTPSTPGAGALAAWASDAMAEMLRAGITCFADMYFFPEEVA
                     RLARQHHLRVSLGLPIADARTPWAADADEHLDKAARLWDEYRDDPLILPCFAPHSPYA
                     VSRATFARLRRLVDQLDAPLAMHLHESQSEVHDSVNAHGERPLSWLADEGLLRPGFAA
                     VHMSWLTDEDIDLAARSGISVVHCPQSNQRLGSGTCPVNRLREAGVNVALGTDGAASV
                     GALDLLAEARAACLLANGSAHSPQALGAHDALAMATLGGARALGLADEIGSITPGKAA
                     DLICIDLSATACHPVGDAADAIVFAATRDSVADVWVAGRAMVREHRLLAFDAAELAAI
                     AREWPARIATGVAA"
     gene            1045..1770
                     /gene="ubiG"
                     /locus_tag="PKN91_07520"
     CDS             1045..1770
                     /gene="ubiG"
                     /locus_tag="PKN91_07520"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HNS27957.1"
                     /translation="MSQAGMHYGADAAELAKFDALAHRFWDPRGEFRPLHSLNPVRAQ
                     FIAGRVRLPGARVLDVGCGGGLLAEALADAGASVTAIDLAPSMIEVARLHALERGVTI
                     DYRVMGAEALLAAGEPPYDIVTCMEMLEHVPDPAALIGCFARLLRPGGELFISTINRN
                     AKSFALGIVAAEYVLRLLPRGTHEYARFIRPSELAAMARPAGCTLIDLAGIAYDPFSG
                     RSSLTTDTAVNYLAHLRREGGTS"
     gene            1770..2444
                     /gene="gph"
                     /locus_tag="PKN91_07525"
     CDS             1770..2444
                     /gene="gph"
                     /locus_tag="PKN91_07525"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01449.2"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HNS27958.1"
                     /translation="MRETSRAPVGAVLFDLDGTLLDTAPDMVGALNTLRREQDLAPVP
                     FAIARPYVSHGSSGLLRVGFPDADADEIERLRERFLALYNDRLADETTLFPGGDILLA
                     HLEAAALPWGVVTNKPGWLAAPLLAELGLEPRMAVLVSGDTLPERKPHPRPLLHAAEA
                     IGIDPLACVYVGDALRDIQAARAAGMRPVVASFGYVAAADAHHLWPAEAWIDALTDLI
                     GWLGSA"
     gene            2441..3724
                     /locus_tag="PKN91_07530"
     CDS             2441..3724
                     /locus_tag="PKN91_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNS27959.1"
                     /translation="MSTAVIAAIGIGGFLLGALIGWLVAQLRAARHAAGLGAALEAAR
                     ARLQADEERAQAQVALLTQSEARLRAAFDELASESLRANSEVFLALARESLGREHATV
                     QGALKERESAFNQLIEPIRAALARTEAEVQALERERREAFTTLRTQIESLTSGQAQLA
                     RETRNLVTALRRPEVRGRWGELTLRRVVELAGLTEHADFTEQAQVATGSGALRPDLVV
                     HMPEERDLVVDAKTPLDAYLEAVEADNDEARRLALGRHAQQVEARVRELAGKAYWSQF
                     ARSPEFAVLFLPGDQFLSSALTERPDLIDNALKQSVVIATPSTLMALLKAVAYGWRQA
                     QVAQNAAEIRDLGQELYRRLGNFSGHLAKVGQRLGNAVESYNSAVGSLERQVMPQARR
                     FTELGVTGDAALANLEPVERLVREPASAPGDNNEP"
     gene            3721..4428
                     /locus_tag="PKN91_07535"
     CDS             3721..4428
                     /locus_tag="PKN91_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27960.1"
                     /translation="MSVPAAPPPPRSAGARYFAWLYSESAARDGVAALLAIEHEIMTG
                     ARGELDHAVAHARLAWWQDECARLHAAQPVHPAALAARDAFLAAGLPPPDMRMLPEFA
                     AARLACASLGRETTAGAAAQDAARWTEALFLPLVALSAAVARVDTGTAAAELRALGVA
                     LCTHEQLRDEATRTALRTTLAALPLPIRAALRALIVWSTIALRPVAVSRGALLAENWI
                     AWRAARAAAPPAQRSAG"
     gene            4425..5198
                     /locus_tag="PKN91_07540"
     CDS             4425..5198
                     /locus_tag="PKN91_07540"
                     /inference="COORDINATES: protein motif:HMM:NF006509.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HNS27961.1"
                     /translation="MNTTFEPRLHRAAPDELVGRVIAITGAGDGIGRAVALACAAHGA
                     EVVLTGRTVGKLEKVYDEIVAAGHAEPIIAPLDLEKATARDYDALADAIGTRYGRLDG
                     LLHNAGLLGDLAPIEHYDVPLWTRVLHVNVTAAFALTQVLLPQLRAAADASVLFTTSS
                     VGRRGRAYWGAYAVSKFAVEGLTQVLADELHGTSSVRVNAINPGATRTAMRRTAYPAE
                     DPATVTPPAAITAAYVCLLGPASKGMTGLSVDAQAPRER"
     gene            complement(5110..6042)
                     /locus_tag="PKN91_07545"
     CDS             complement(5110..6042)
                     /locus_tag="PKN91_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HNS27962.1"
                     /translation="MRPRTASPASSLRLTIRIAEAAAAVPRRTCMTGTHASGDRLQKH
                     LASLGLASRREAEAWIRAGRVTVNGVVATLGARVGPRDEVRLDGRPVRRRAAPAKTTA
                     YLAHRSPGEPLQSPDESGHEPLLARMPRKAGRRFIAVSPMPRVDGGLEVFTPDGELAA
                     RLQRAARQMEIEFRVRIHGELPAERLAALAEGVLDSGERLTVLGCEAGGGEGSNRWYA
                     IVTRGASGKAVRQLFERQGATVSRVLRVRFGTLELDRDLPRGRSRAMTDAELAALVGT
                     PPPAEPSARAAPARPRSGRSSPSKRVPAGRRTRR"
     gene            complement(5984..6724)
                     /gene="scpB"
                     /locus_tag="PKN91_07550"
     CDS             complement(5984..6724)
                     /gene="scpB"
                     /locus_tag="PKN91_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNS27963.1"
                     /translation="MSDTEPRDTGLRNILEAALLAAGRPLAIADLAGLFDEAERPATV
                     ELRAALEALAQDYEDRALQLLETASGWRLQVRAQFAPQVSRLWPERPQKYSRALLETL
                     ALIAYRQPITRAEIEAVRGVAVNPNIVRTLTERGWIRVVGQRDVPGHPELLGTTREFL
                     DYFGLKSLDQMPTLAELTAMTDVSPQLDLPVADAQGLPEPAADSEAALDDLALEDDGG
                     DGEGDDDELDAAADSKPGLVAAPHDPDR"
     gene            complement(6721..7548)
                     /locus_tag="PKN91_07555"
     CDS             complement(6721..7548)
                     /locus_tag="PKN91_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HNS27964.1"
                     /translation="MPFAMVHGEAVTEVPKDLYIPPQALEVFLDAFEGPLDLLLYLIR
                     RQNLDILDIPLADITRQYMQYIELMQELQLELAGEYMLMAATLAEIKSRMLLPRSQDA
                     LAGEEVDPRADLVRRLQEYERFKRAAEGLDALPRLERDVFVASAEFHDRKVVRLLPQV
                     QLQEMLHAFREVVQRAEMFAHHHVQRERLSVRARMSDVLSALEGGGFVEFGQLFRPEE
                     GRMGVTVTFVAILELLREGLIDFVQAEPYAPLHVRAAPPGHSGLQLVADNAPGEAMT"
     gene            complement(7626..8843)
                     /locus_tag="PKN91_07560"
     CDS             complement(7626..8843)
                     /locus_tag="PKN91_07560"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNS27965.1"
                     /translation="MSAVAQARRVLSGMRPTGALHLGNFHGALANWIELQYQYECYFF
                     VADWHALTTDYADTGALPAHIHEMLIDWLAAGLNPGVATLFVQSHVPEHAELHLLLSM
                     ITPLGWLERVPTYKDQQAELKEKDLATYGFLGYPLLQSADILIYRPAFVPVGEDQVAH
                     VEITREVARRFNHLYGSEPDFEAKAEKAAKGLGGRLSAQYRQLRRRYQEAGDAAALEQ
                     ARAIVHANNRITVADRERLLGWLEGAGVSILPEPQVLLTKSPKVPGLDGRKMSKSYGN
                     TIGLREDPDAVAKKLRTMQTDPARVRRHDPGDPLKCPVWDMHKIYSDEATREWVWNGC
                     RSGSIGCLECKQPVIEKVVEFVTGMRKRAQEYQDNPELLRTILVEGAEKAREAAHDTM
                     DEVRRVMHLRGGL"
     gene            complement(8956..9591)
                     /locus_tag="PKN91_07565"
     CDS             complement(8956..9591)
                     /locus_tag="PKN91_07565"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNS27966.1"
                     /translation="MSSYFELHPVNPQLRILREAVQVIRRGGVVAYPTDSCYALGCHI
                     GDKDALERIRRIRHADRHHHFTLMCRDLSEIGRYARVDTWQFRLLKAVTPGPYTFLLP
                     ATRETPRRLQHDKRRTIGIRVPAHPVPRLLLTELDEPIMTSTLLLPGDDTPLTNGAEI
                     HERLGHELDMVLDGGYCGTEPTTVVDLAVRPAVIVRVGKGPVEPLGLADAD"
     gene            9630..10172
                     /gene="ispZ"
                     /locus_tag="PKN91_07570"
     CDS             9630..10172
                     /gene="ispZ"
                     /locus_tag="PKN91_07570"
                     /inference="COORDINATES: protein motif:HMM:NF016189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HNS27967.1"
                     /translation="MQQILDFAPLVVFLVAYYLKGMYFATAALMIAMLLLLVADKLLL
                     GRVPTMHWVSAVLVLLFGAATLLLQDQRFIQWKPTIFFWLVSVALLGSRWIGERPLVE
                     RMLAPALGADARLPRETWQRINLLWVGFYAALGVLNIAVAFNAPERTWVNFKVFGLTA
                     LTFVFIVAQLPWILKRAHPA"
     gene            10169..10480
                     /locus_tag="PKN91_07575"
     CDS             10169..10480
                     /locus_tag="PKN91_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HNS27968.1"
                     /translation="MSANPDEQAERLARLRSALEAAFAPQSLTIRDDSAAHIGHAGAR
                     EGGHYQVTIVATAFAGRSVLERHRLVYAALSALMGHGVHALSIDARAPSEQHHLQNVT
                     N"
     gene            10489..11307
                     /locus_tag="PKN91_07580"
     CDS             10489..11307
                     /locus_tag="PKN91_07580"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNS27969.1"
                     /translation="MKSARLLAVVMLAALAAACKPGTEGPAATADNGAVATVNGTAIT
                     RQLFDTYAQAVTGKPSSELTDEQRTQVLDNLIRAELIGTQAEKDGVTKTPETAAMLQL
                     ARLNVLQQEMSEKFLADKAPTDQEMRAEYETQVAALPRNEYKARHILVATQEFAANLI
                     SQLDKGAKFETLAKRESLDSSKENGGDIGWFTPDRMVPQFSAAVVALEKGQYTKAPVQ
                     TQYGWHVIRLDDVRDVQPPPFDAVKDRLEQIVQTKKFKAYADELMAAAKIEKNP"
     gene            complement(11356..>12719)
                     /gene="ligA"
                     /locus_tag="PKN91_07585"
     CDS             complement(11356..>12719)
                     /gene="ligA"
                     /locus_tag="PKN91_07585"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNS27970.1"
                     /translation="RPSGLGTVEGGVMPMRQSELLAQLRSYGLRTCPEAKVVRGIEGC
                     LGYFARLGAARARLPYQIDGVVYKVERRDWQEQLGFVSRAPRWALAHKFPADEAETTV
                     RAVEFQVGRTGAVTPVARLEPVFVGGVTVSNATLHNMDEVGRKDVRVGDTVVIRRAGD
                     VIPEVVRVVPGRRHADAQPVQLPAQCPVCGSSVVKEEGEAIARCTGGFRCRAQRREAL
                     KHLAGRRALDIDGLGDRIVDQLVERELVRTPADLYALDAATLAELDRMGEKSAQNLIA
                     AIDASRRTTLPRLLFALGIRDVGESTALALAQHFGYIEALMAADEVQIQQVPDVGPVV
                     AAHVARYFADPANRELIAELRARGVEWPPMAAPRAAEMPLGGLTFVLTGRLTQLTREA
                     ATEALLALGAKVAGSVSKKTSYVVAGDEAGSKLDKARALGVPVIDEAALAEVLARRRR
                     PGA"
BASE COUNT         1868 a         4611 c         4345 g         1895 t
ORIGIN      
        1 agcgcggcgt catactccgt ccactccggg cgcgggtgcg cttgcggcgt gggcctcgga
       61 cgcgatggcc gaaatgctgc gtgccggcat cacctgcttc gccgacatgt atttctttcc
      121 cgaggaggtc gcgcggctcg cgcgccagca ccacctgcgc gtgtcgctcg gcctgccgat
      181 cgcggacgcc cgcacaccct gggccgccga tgccgacgag cacctcgaca aggccgcaag
      241 gctctgggac gagtaccgcg acgatccgct catcctgccc tgcttcgcgc cgcattcgcc
      301 gtacgcggtc agccgcgcca ccttcgcgcg cctgcgacgc ctggtcgatc aactcgatgc
      361 accgctcgcg atgcacctgc acgagtccca gtccgaagtg catgacagcg tcaatgccca
      421 cggcgagcgc ccgctgtcct ggctcgcaga cgagggcctg ctgcgcccgg gcttcgccgc
      481 ggtgcacatg tcctggctca ccgacgagga catcgatctt gcggcgcgct cgggcatctc
      541 cgtggtgcat tgcccgcaat cgaatcagcg cctcggcagc ggtacctgcc cggtcaaccg
      601 gttgcgcgag gcgggcgtga acgtcgcgct cggcaccgac ggggccgcca gcgtcggcgc
      661 gctcgacctg ctcgccgagg cccgcgcggc ctgcctgctc gcgaacggca gtgcgcacag
      721 tccgcaggcg cttggtgcgc acgacgcgct cgccatggcg acgctcggcg gtgcccgggc
      781 gctgggcctc gcggacgaga tcggctcgat cactcccggc aaggcggccg acctgatctg
      841 catcgacctg tcggccacgg cctgccaccc ggtcggcgac gcggccgatg ccatcgtctt
      901 tgccgcgacg cgcgactcgg tcgcggacgt gtgggtcgcg ggccgggcca tggtacgcga
      961 gcatcgcctg ctcgccttcg atgccgcgga gctcgcggcc atcgcgcgcg agtggcccgc
     1021 gcgcatcgcc accggagtcg ccgcatgagc caggccggca tgcattacgg cgccgacgcc
     1081 gccgaactcg ccaagttcga cgccctcgcg catcgcttct gggacccgcg cggcgagttc
     1141 cgtccgttgc acagcctgaa cccggtgcgc gcacagttca tcgcgggacg cgtgcggctc
     1201 cccggcgcac gcgtgctcga cgtcggctgc ggcggcggcc tgctcgccga ggcgctcgcc
     1261 gacgccggcg ccagcgtgac ggcgatcgac ctcgcgccgt cgatgatcga agtggcgcgc
     1321 ctgcacgcgc tcgagcgggg cgtgacgatc gactatcgcg tcatgggcgc cgaggcactg
     1381 cttgccgcgg gcgagccacc ctacgacatc gtcacctgca tggaaatgct cgagcacgta
     1441 ccggaccctg ctgcgctcat cggctgcttc gcgcggctcc tgcgtcccgg cggcgagctg
     1501 ttcatctcga cgatcaaccg caacgcgaag tccttcgcgc tcggcatcgt cgcagccgaa
     1561 tacgtacttc ggcttctgcc gcgcggcacg cacgagtacg cgcgcttcat ccgcccgtcg
     1621 gaactcgcgg ccatggcgcg gccggcgggc tgcaccctca tcgaccttgc cggcatcgcc
     1681 tacgatccct tcagcgggcg aagttccctg accaccgaca cggcagtgaa ctacctggcg
     1741 cacttgcggc gcgagggcgg cacatcgtga tgcgcgaaac ctcgcgcgcg ccggtcggcg
     1801 ccgtattgtt cgatctcgac ggcacgctgc tcgacaccgc gcccgacatg gtgggcgcgc
     1861 tcaacacgct gcgccgcgaa caagacctcg cgccggtacc gttcgcaatc gcacgtccgt
     1921 acgtatcgca cggttcgagc ggcttgctcc gcgtcggctt ccccgatgcg gacgcggacg
     1981 agatcgagcg gctgcgcgag cgcttcctcg ccctctacaa cgatcgcctc gccgatgaga
     2041 ccaccctctt tccggggggc gatatcctgc tcgcgcatct cgaggccgcg gcgctgcctt
     2101 ggggcgtagt gaccaacaag cccggctggc tcgccgcacc gctccttgcc gaactcggcc
     2161 tcgagccgcg catggccgtg ctcgtgagcg gcgacaccct gccggaacgc aagccgcacc
     2221 cgcgcccatt gctgcatgcg gccgaagcga tcggcatcga cccgctcgcc tgcgtgtacg
     2281 tcggcgatgc gctgcgcgac atacaggccg cacgcgcggc cggcatgcgg ccggtggtcg
     2341 cctcgttcgg ctatgtggcg gccgcggacg cccatcacct gtggccggcg gaggcgtgga
     2401 tcgatgcgct aaccgacctg atcggctggc tgggaagcgc atgagtacgg cggtcatcgc
     2461 ggcgatcggc atcggcggct tcctcctcgg ggcgctgatc ggctggctcg tggcccagtt
     2521 gcgcgcggcg cgacacgctg ccggactcgg cgctgcgctc gaagccgcca gggcgcgact
     2581 gcaggccgac gaggagcgcg cgcaggcgca ggtcgcgctg ctgacgcaga gcgaggcgcg
     2641 gctgcgcgcc gcgttcgacg agctcgcctc ggaatcgctg cgcgcgaaca gcgaagtctt
     2701 cctggcgctg gcgcgcgaat cgctggggcg cgagcacgcc accgtgcagg gcgcgctgaa
     2761 ggagcgcgag agcgcattca accagctgat cgagccgatc cgcgcggcgc tcgcccgcac
     2821 cgaggccgaa gtgcaggcac tcgagcgcga gcgccgcgag gcattcacga cgctgcgcac
     2881 gcagatcgag tcgctgacga gcggccaggc ccagctcgcg cgcgagacgc gcaacctggt
     2941 caccgcgctg cgccggcccg aagtgcgcgg ccgctggggc gagctcacgc tgcgtcgggt
     3001 cgtcgaactc gcgggactca ccgagcacgc cgacttcacc gaacaggcgc aggtcgcgac
     3061 cgggtccggc gcgctgcgac ccgacctcgt cgtgcacatg cccgaggagc gtgacctcgt
     3121 ggtcgacgcc aagacgccac tcgatgcgta tctcgaggcg gtcgaggccg acaacgacga
     3181 agcgaggagg ctcgcgctcg ggcgccatgc acagcaggtc gaagcgcgcg tgcgcgaact
     3241 cgcgggcaag gcctactggt cgcagttcgc gcgcagcccc gagttcgcgg tgctgttcct
     3301 gcccggcgac cagttcctgt cgtcggcgct cacggagcgc cccgacctga tcgacaacgc
     3361 gctgaagcag agcgtggtga tcgcgacccc ctcgacgctg atggcgctgc tgaaggcagt
     3421 cgcctacggc tggcgccagg cgcaggtcgc gcagaatgcc gccgagatcc gcgacctcgg
     3481 ccaggagctc tatcgacgcc tcggcaactt ctccggccac ctcgccaagg tcggccagcg
     3541 cctcggcaac gcggtggagt cgtacaacag cgcggtaggc tcgctcgagc gccaggtgat
     3601 gccgcaggcg cgacgcttca ccgagctcgg cgtgaccggc gatgccgcac tggcgaacct
     3661 cgagccggtc gagcggctgg tgcgcgaacc ggcgtctgcc cccggcgaca acaacgagcc
     3721 gtgagcgttc ccgcggctcc accgccgccc cgcagtgccg gcgcgcgcta tttcgcctgg
     3781 ctatacagcg aatcggcggc gcgcgacggc gtcgccgcgc tgctcgcgat cgaacacgaa
     3841 atcatgacgg gcgcgcgcgg cgagctggat catgcggtgg cgcacgcgcg cctcgcgtgg
     3901 tggcaggacg agtgcgcgcg cctgcacgcc gcgcagcccg tgcacccggc cgccctcgcc
     3961 gcccgggatg cgttcctggc cgccggcctg ccgcctccgg acatgcggat gctgccggag
     4021 ttcgcggccg cgagactcgc ctgcgcgagc ctcgggcgag agaccacggc cggggctgcg
     4081 gcgcaggatg ccgctcgctg gaccgaggcg ctcttcctgc cgctcgtcgc actgtctgcc
     4141 gcagtggccc gcgtggacac cggcaccgcg gccgcggagc tgcgcgcgct cggcgtcgcg
     4201 ctctgcacgc acgagcagct gcgagacgag gccacgcgca cggcgctgcg caccacgctc
     4261 gcggcactcc cgctccccat acgtgcagcg ctgcgtgcgc tcatcgtgtg gtccacaatc
     4321 gccctgcggc ccgtcgcggt ctcgcgtggc gcgctgctcg ccgagaactg gatcgcctgg
     4381 cgcgccgcac gcgcggccgc accccctgcc cagaggtctg ccggatgaat acaactttcg
     4441 aaccgcggct gcatcgcgcc gcccccgatg agcttgtcgg tcgcgtgatc gcgatcaccg
     4501 gcgcgggcga tggcatcggc cgcgcggtcg cgctcgcctg cgccgcgcac ggcgccgaag
     4561 tggtgctgac cgggcgcacg gtcggcaagc tcgaaaaagt ctacgacgag atcgtcgccg
     4621 cgggtcatgc cgaacccatc atcgcaccgc tcgatctcga gaaggccacg gcccgcgact
     4681 acgacgcact cgcggacgcg atcggcacgc gctatggccg cctcgatggc ctgcttcaca
     4741 acgcgggcct gctcggcgat ctcgcgccga tcgagcatta cgatgtgccg ctctggacgc
     4801 gcgtgctgca cgtcaacgtc accgcggcat tcgcgctgac ccaggtcctg ctgccgcagc
     4861 tgcgggcggc ggccgatgcg agcgtactgt tcacgacgag cagcgtcggg cggcgcggtc
     4921 gcgcgtactg gggcgcctac gccgtctcga aattcgcggt cgagggcctc acgcaggtgc
     4981 tcgccgacga actgcacggc acgagctcgg tgcgcgtcaa cgcgatcaat ccgggcgcga
     5041 cgcgcacggc aatgcgccgc acggcgtatc cggccgagga tcccgcgacg gtgacgccgc
     5101 cggcggcgat caccgccgcg tacgtctgcc tgctgggacc cgcttcgaag gggatgaccg
     5161 gcctgagcgt ggacgcgcag gcgccgcgcg agcgctaggc tcggcgggcg gcggcgtacc
     5221 gaccagcgca gcgagctccg catcggtcat tgcgcgtgaa cgaccccgcg gcagatcgcg
     5281 gtcgagttcg agggtgccga aacgtacgcg cagcacgcgg ctgaccgtcg cgccctggcg
     5341 ctcgaacagc tggcgcaccg ccttgccgct cgcgccacgc gtgacgatcg cgtaccagcg
     5401 gttgctgcct tcgccaccgc ccgcctcgca gccgagtacc gtgagccgct cgccgctgtc
     5461 gagcacgcct tcggccagcg ccgccaggcg ctcggcgggc agctcaccgt ggatgcgcac
     5521 gcggaattcg atctccatct gccgcgccgc gcgctgcagt cgcgcggcga gctcaccgtc
     5581 cggcgtgaag acctcgagcc cgccatcgac acgcggcatc gggctcacgg cgatgaaacg
     5641 ccgtccggcc ttccgcggca tgcgcgcgag cagcggctcg tggccggact cgtcggggct
     5701 ctgcagaggc tcgcccggcg agcggtgcgc aagataggcc gtcgtctttg cgggggcagc
     5761 gcggcggcgc acggggcggc cgtcgagacg cacttcatcg cgcggaccga cccgcgcgcc
     5821 cagcgtggcg acgacgccat tgacggtgac gcgcccggca cggatccacg cctcggcctc
     5881 gcggcgggat gcgagcccca ggctcgcgag gtgcttctgc aggcgatcgc cgctggcgtg
     5941 cgtgccggtc atgcaggtgc gacgcgggac cgcggccgcg gcctcagcga tccggatcgt
     6001 gaggcgcagc gacgaggccg ggcttgctgt ccgcggccgc atcgagctcg tcatcgtcgc
     6061 cctcgccgtc gccgccgtca tcctcgagcg ccaggtcatc gagcgccgcc tcgctgtccg
     6121 ccgccggctc cggcaggcct tgcgcatcgg cgaccggcag atcgagctgc gggctgacat
     6181 cggtcatcgc cgtcaactcg gcgagggtcg gcatctggtc gaggctcttc aggccgaagt
     6241 agtcgaggaa ctcgcgggtc gtgccgagca gttccggatg ccccggcaca tcgcgctggc
     6301 cgacgacgcg gatccagccg cgctcggtca gggtgcgcac gatgttggga ttcactgcga
     6361 cgccacgcac ggcctcgatc tcggcacgcg tgatcggctg ccggtaggcg atcagcgcca
     6421 gggtctcgag cagcgcgcgc gagtacttct gcggacgctc cggccacagg cgcgacacct
     6481 gcggcgcgaa ctgcgcgcgc acctgcaggc gccagccgct cgcggtctcg agcagctgca
     6541 gcgcacggtc ctcgtagtcc tgcgccagcg cctccagcgc ggcacgcagt tccaccgtcg
     6601 ccggacgctc ggcctcgtcg aacaggcccg caagatctgc aatcgccagc ggacggcccg
     6661 ctgccagcag ggccgcctcg aggatgttgc gcagtccggt atcgcgcggt tcggtatcgc
     6721 tcatgtcatt gcctcgccgg gcgcgttgtc cgcgaccagt tgcagtccgc tgtggcccgg
     6781 cggcgccgcg cgcacgtgca acggcgcata cggctccgcc tggacgaaat cgatgagtcc
     6841 ctcgcgcagc aactcgagga tcgcgacaaa ggtgacggtc acgcccatgc ggccttcctc
     6901 ggggcggaac agctggccga attcgacgaa gccgccgccc tcgagtgccg agagcacgtc
     6961 gctcatgcgg gcgcgcaccg acaggcgctc gcgctggaca tggtggtgcg cgaacatctc
     7021 ggcgcgctgc acgacctcgc ggaaggcatg cagcatctcc tgcagctgca cctgcggcag
     7081 gagtcgcacg accttgcggt catggaactc ggcgctcgcc acgaacacgt cgcgctcgag
     7141 gcgcggcagc gcatccagcc cctcggcggc gcgcttgaag cgctcgtact cctgcagccg
     7201 gcgcacgagg tcggcgcgcg gatcgacttc ctcgcccgcc agcgcatcct gcgagcgcgg
     7261 cagcagcatc cgcgacttga tctcggcgag cgtggcggcc atcagcatgt actcgcccgc
     7321 gagttcgagc tgcaactcct gcatcagctc gatgtactgc atgtactgcc gggtgatgtc
     7381 ggcgagcggg atgtcgagga tgtcgaggtt ctggcggcga atcaggtaca gcagcagatc
     7441 gagcggcccc tcgaaggcgt cgaggaagac ctcgagcgcc tgcggcggaa tgtacaggtc
     7501 cttcgggacc tcggtgaccg cctcgccatg caccatggcg aacggcatct ccgcctgctg
     7561 cggcgcggcg gcggccgcgg cgggatccgc tcccgggttc gagacgacga gtttcaggtc
     7621 gggtttcaca ggccaccccg aagatgcatg acccgacgca cctcgtccat cgtgtcgtgc
     7681 gcggcctccc gcgccttctc ggccccctcg acgaggatcg tgcgcaggag ttccgggttg
     7741 tcctgatatt cctgcgcgcg cttgcgcatg ccggtgacga actcgaccac cttctcgatc
     7801 accggctgct tgcactcgag gcagccgatg ctgcccgagc ggcagccatt ccagacccat
     7861 tcgcgcgtcg cctcgtcgga gtagatcttg tgcatgtccc acaccgggca cttcagcggg
     7921 tcgcccggat cgtggcggcg cacgcgcgcc ggatcggtct gcatcgtcct cagcttcttc
     7981 gcgacggcat ccggatcctc gcgcagtccg atcgtattgc cgtaggactt cgacatcttg
     8041 cggccgtcga gcccgggcac cttcggcgat ttcgtgagca acacctgcgg ctcgggcagg
     8101 atcgacacgc ccgcgccctc gagccagccg agcaggcgtt cgcggtcggc cacggtgatg
     8161 cggttgttcg catgcacgat cgcacgcgcc tgctcgagcg ccgccgcgtc gccggcttcc
     8221 tggtagcggc ggcgcagctg ccggtattgc gcgctcagcc tgccgccgag ccccttcgcg
     8281 gccttctcgg ccttggcctc gaagtccggc tcgctgccgt acaggtgatt gaagcggcgc
     8341 gcgacctcgc gcgtgatctc gacgtgcgcg acctggtcct cgcccaccgg cacgaaggcc
     8401 ggccggtaga tcaggatatc ggcggactgc agcagcgggt agccgaggaa gccgtaggtc
     8461 gcgagatcct tctccttcag ctcggcctgc tggtccttgt aggtcggcac ccgctcgagc
     8521 cagccgagcg gcgtgatcat cgacagcagc agatgcagct ccgcgtgctc gggcacgtgc
     8581 gactgcacga acagcgtggc gacgccgggg ttcagcccgg cggccagcca gtcgatcagc
     8641 atctcgtgga tgtgtgccgg cagcgcgccg gtgtccgcat agtcggtggt cagcgcatgc
     8701 cagtcggcca cgaagaagta gcactcgtac tggtactgca gctcgatcca gttggcgagc
     8761 gcaccatgga aattgccgag gtgcagcgcc ccggtcggtc gcatgcccga gagcacgcgc
     8821 cgcgcctgcg caacggcgct caagcgggcc tcgccaagcg ggtgttcggt cgggtcgaca
     8881 ggggactcgg cacgtcgggt cggggcttca ctggtggagc gcggtgcgcg cgccggatga
     8941 ttatagccca gtgcctcaat cggcgtcggc gaggcccagg ggctcgaccg gacccttgcc
     9001 gacacgcacg atgaccgcag ggcgcaccgc gaggtcgacg acggtggtcg gctcggtgcc
     9061 gcagtagccg ccgtcgagca ccatgtcgag ctcgtgcccg aggcgctcgt ggatctcggc
     9121 accattggtg agaggcgtgt cgtcgcccgg cagcagcagc gtggaggtca tgatcggctc
     9181 atcgagctcg gtcagcagca ggcggggcac ggggtgggcc ggcacgcgga tgccgatcgt
     9241 gcgccgcttg tcgtgctgca ggcgccgcgg cgtctcgcgc gtggccggca acaggaaggt
     9301 atacggtccg ggcgtcacgg ccttcagcag ccggaactgc caggtgtcga cgcgggcgta
     9361 gcggccgatc tcgctgagat cgcggcacat cagcgtgaag tgatggtggc ggtcggcatg
     9421 gcggatgcgc cggatgcgct cgagcgcgtc cttgtcgccg atgtggcagc cgagggcgta
     9481 gcacgagtcg gtcggatagg cgaccacccc gccgcgcctg atcacctgca ccgcctcgcg
     9541 caggatgcgc agctgggggt ttaccggatg caactcgaaa tatgaggaca tgcaggtatc
     9601 atatcggccc cgccgccgac cgttcgccca tgcagcagat cctggacttc gcgcccctcg
     9661 tcgtcttcct cgtcgcctac tacctgaagg gcatgtattt cgcgaccgcg gcactgatga
     9721 tcgcgatgct gctgctgctc gtggccgaca agctgctgct cgggcgcgtg ccgacgatgc
     9781 actgggtctc ggcagtgctc gtgctgctgt tcggcgcggc gacactgctg ctgcaggacc
     9841 agcgcttcat ccagtggaaa ccgaccatct tcttctggct cgtgagtgtc gcgctgctcg
     9901 gcagccgctg gatcggcgag cgcccgctcg tcgagcgcat gcttgccccc gcgctcggcg
     9961 ctgacgcccg gctgccgcgc gagacctggc aacgcatcaa cctgctgtgg gtcggcttct
    10021 acgccgcgct cggcgtgctc aatatcgccg tcgcgttcaa cgcgccggag cggacctggg
    10081 tgaacttcaa ggtgttcggc ctcacggccc tcactttcgt gttcatcgtc gcgcagctgc
    10141 cgtggatact gaaacgggcg cacccggcat gagcgcgaac ccggatgagc aggccgagcg
    10201 gctcgcccgc ctgcgcagtg cgctcgaggc ggcctttgcg ccgcagtcgc tcaccatccg
    10261 tgacgacagt gccgcgcaca tcggccacgc cggcgcacgc gaaggcggcc actaccaggt
    10321 gacgatcgtg gccacggcct tcgccggacg ctccgttctg gaacgccatc gtttggtcta
    10381 tgctgcgctc tccgcgctga tgggccacgg cgtccacgca ctgtcgatcg atgccagagc
    10441 accttcggaa caacatcacc ttcaaaacgt aaccaactga gatatttcat gaaatcagct
    10501 cgactcctcg ccgtggtcat gctcgccgcg ctggccgccg cctgcaagcc cggtaccgag
    10561 ggccccgccg ccaccgccga caacggcgcc gttgccaccg tcaacggcac ggcgatcacg
    10621 cgccagctgt tcgacaccta tgcgcaggcc gtcacgggca agccgtcgtc cgaactgacc
    10681 gacgagcagc gcacgcaggt gctcgacaac ctgatccgcg ccgaactgat cggcacgcag
    10741 gcagagaaag acggcgtcac gaaaactccg gagacggcgg ccatgctcca gctggcccgc
    10801 ctcaatgtcc tgcagcagga gatgtcggag aagttcctcg ccgacaaggc gccgaccgat
    10861 caggaaatgc gcgcggagta cgagacgcag gtcgccgcgc tgccccggaa cgaatacaag
    10921 gcccgccaca tcctggtggc cacgcaggag ttcgcggcca atctgatctc gcagctcgac
    10981 aagggcgcga agttcgagac gctcgcgaag cgcgaatcgc tcgactcctc gaaggaaaac
    11041 ggcggcgata tcggctggtt cacccccgat cgcatggttc cgcagttctc cgctgccgtc
    11101 gtggcgctcg agaagggcca gtacaccaag gcgccggtgc agacgcagta cggctggcac
    11161 gtgatccgcc tcgatgacgt gcgcgacgtg cagccgccgc cgttcgacgc ggtcaaggac
    11221 cgcctcgagc agatcgtgca gacgaagaag ttcaaggcct atgccgatga attgatggcc
    11281 gcggcgaaga tcgaaaagaa tccctgatcc gcgcaccgcg ggcgggcagg acccgcgatc
    11341 cgacggccgg tcgcctcagg cgcccggccg tcgtcgtctg gcgagcacct cggcgagcgc
    11401 cgcctcgtcg atgaccggca cgccgagcgc acgcgccttg tcgagcttgc tgccggcctc
    11461 gtcgcccgcg accacatagc tcgtcttctt cgacaccgag ccggcaacct tcgcgccgag
    11521 cgcaagcagc gcctcggtcg ccgcctcgcg cgtcagctgc gtcagtctgc ccgtcagcac
    11581 gaaggtcagg ccgccgagcg gcatttcggc cgcgcggggt gcagccatgg gcggccattc
    11641 gacgccgcgc gcccgcagct cggcgatcag ttcgcgattg gcgggatccg cgaaatagcg
    11701 cgccacatgg gccgccacca caggtccgac gtcgggaacc tgctggatct gcacttcgtc
    11761 cgccgccatc aacgcctcga tgtagccgaa gtgttgcgcg agcgcgagcg cagtcgactc
    11821 acccacgtcg cggatgccga gcgcgaacag gagccgcggc agcgtggtcc tgcggctcgc
    11881 gtcgatggcc gcgatgaggt tctgcgccga cttctcgccc atgcgatcga gctccgcgag
    11941 cgttgccgcg tcgagcgcgt acaggtcggc cggcgtgcgc acgagttcgc gctcgaccag
    12001 ctggtcgacg atgcggtcgc cgagcccgtc gatgtcgagg gcccggcgcc ccgcgagatg
    12061 cttcagtgcc tcgcgccgct gggcgcggca gcgaaagccc ccggtgcagc gcgcgatcgc
    12121 ctcgccctcc tccttgacga ccgacgaccc gcagaccgga cactgcgcgg gcaattgcac
    12181 cggctgcgca tcggcatggc gccgacccgg cacgacgcgc acgacctccg ggatcacgtc
    12241 tcccgcccgg cggatcacca ccgtgtcgcc gacacgcacg tccttgcgcc cgacctcgtc
    12301 catgttgtgc agcgtcgcgt tgctgaccgt tacgccgccg acgaataccg gttcgaggcg
    12361 cgcgacggga gtcaccgctc ccgtgcgccc cacctggaat tccaccgccc tgacggtcgt
    12421 ctcggcctcg tcggccggaa acttgtgcgc cagagcccag cgcggcgcac gcgagacgaa
    12481 accgagctgc tcctgccagt cgcgccgctc gaccttgtag acgacgccgt cgatctggta
    12541 cggcaggcgt gcccgggccg cgccgaggcg cgcgaagtag ccgaggcaac cctcgatgcc
    12601 gcgcacgacc ttggcttccg ggcaggtgcg cagtccgtag ctgcgcagct gcgccagcag
    12661 ctcgctctgc cgcatcggca tcaccccgcc ctcgacggtg ccgaggccac tcgggcggt
//
LOCUS       DAOQVY010000067        12661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_5609_length_12661_cov_5.265746, whole genome shotgun sequence.
ACCESSION   DAOQVY010000067 DAOQVY010000000
VERSION     DAOQVY010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12661
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5609_length_12661_cov_5.265746"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="PKN91_07590"
     CDS             <1..1145
                     /locus_tag="PKN91_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNS27971.1"
                     /translation="GRLVHDHDGGEQLLPYGQHDLEVIHSVSREALGRTLVEAAAEQR
                     GIALHFGQTCVGVDVARDALLFGTADKVQRRVPLVPTIATDGAGSAVRVALAAAGAIT
                     VREDMLAHDYRELTVPPERARGLHPNALHIWPRGGFMLIALPNTDGSFTATLFLAREG
                     SPSFAELTDAAAIRDFFAREFPGAVPLLPRLVEEFAENPQGRLGTVYSEPWHVGGKVL
                     LLGDAAHAIVPFHGQGMNCAFEDVAVLDALLGEHQDFDALFGAFMRARRPDTDAIAQM
                     ALENYLEMRDTVLDPAFQRRKTLGSCLERHYPERFIPRYSMVMFHPEIGYAEALRRGA
                     VQDALLTRIDAAHPGADPEEIADLAAVRELVEARLPRLNRASAART"
     gene            complement(1117..1587)
                     /locus_tag="PKN91_07595"
     CDS             complement(1117..1587)
                     /locus_tag="PKN91_07595"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNS27972.1"
                     /translation="MARNGVIGQDGALPWHLPDDLRRFKALTLGKPILMGRRTWESIG
                     RPLPGRTNLVLTRDPAFRAEGANAVASLEAAVAAAAGHEELMVIGGAQVYALAAPLAF
                     RVHLTAIDAEVEGDTRLPPFDRGEWREVAREHHAADARHAHAMDFVTFERRSRD"
     gene            complement(1620..2189)
                     /locus_tag="PKN91_07600"
     CDS             complement(1620..2189)
                     /locus_tag="PKN91_07600"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNS27973.1"
                     /translation="MSATNKSPMYKVRRSKIHGFGVFATRTIRKGTRIIEYLGDRISH
                     KVADQRYEDKDEDDGHTFLFIVDRGVVIDAGVDGNDARFINHGCDPNCESVIENRRVF
                     IEAIRTIRPGEELNYDYQIGRADSDPPNVDEIFACRCGAATCRGTMLWPAKRPKKRAK
                     RKTGAKRKTGAKRKTGTKSKVGAKKRARR"
     gene            complement(2202..2996)
                     /gene="thyA"
                     /locus_tag="PKN91_07605"
     CDS             complement(2202..2996)
                     /gene="thyA"
                     /locus_tag="PKN91_07605"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNS27974.1"
                     /translation="MQTYLDLLRRVRDHGAHKPDRTGTGTRSLFGCQMRFDLTRGFPL
                     LTTKRVHVKSVVYELLWFLRGDTNVAYLREHGVTIWDEWADAAGELGPVYGSQWRSWP
                     TPDGGHIDQIARVLGELRTNPHSRRIVVSAWNVGELDRMALLPCHALFQFYVADGRLS
                     CQLYQRSADLFLGVPFNIASYSLLTHMIAQQCDLGVGEFIWTGGDTHLYLNHLEQADT
                     QLAREPLPLPELVIARRPETLFDYRFEDFEFRNYQPQPAIKAPVAI"
     gene            3080..3973
                     /locus_tag="PKN91_07610"
     CDS             3080..3973
                     /locus_tag="PKN91_07610"
                     /inference="COORDINATES: protein motif:HMM:NF017269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HNS27975.1"
                     /translation="MFTRNAVPALLSGCTLALLLAACGSPAPSNDAATAAEDAAAALA
                     EKEAALAEREAALAEKEAEQQEQEAARLAAEEAARLESEAAAAAAAAAAAKKPAPAKT
                     STAASKPATSAPASSTAAKPAAPPPPLVVPAGTTLTVALAQALSTKTAKVGDPISAQV
                     VSDVMVGNRVAIPAGSRVSGTVTNVVSGSSKIGGVPALGLRFDTLQLIGGQSMPISGE
                     IVQQGRSDTGMDTAKILGGAAAGAVIGHQVDDDKGKVIGGLLGGAAGAIAAKKTGAEV
                     QLAQDSTLAIALGAPIEVAGR"
     gene            complement(3970..4764)
                     /gene="lgt"
                     /locus_tag="PKN91_07615"
     CDS             complement(3970..4764)
                     /gene="lgt"
                     /locus_tag="PKN91_07615"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNS27976.1"
                     /translation="MIKYPGIDPIALQLGPVRIHWYGIMYLVGFVAGWWLGRRRAARP
                     GSTWKPQDVDDLIFFAMLGVIIGGRVGYVLFYGLGYWALDPLYPLKIWEGGMSFHGGL
                     AGVAVALWLFAWRRRRRTADVYDFIAPLPGIGLFAGRIGNFINGELWGSETTVPWGFE
                     VDGVVRHPTQLYEAALEGLLLFAVIWWFTSKPRPRLAPTGLFLVIYAVSRMLVEFWRV
                     PDAHIGYFAGNWLTMGQLLSLPLLVAGVAMLAWAYARREPSGNYAR"
     gene            complement(4761..5957)
                     /locus_tag="PKN91_07620"
     CDS             complement(4761..5957)
                     /locus_tag="PKN91_07620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNS27977.1"
                     /translation="MLLRLKKGEDRRLRAGHSWVFSNEVDTGATPLSAFTPDLTARIV
                     SDRDAFLGYGYVNPHALISARLLSRDERREPDGALLASRIARALRLRERLHPTPHYRL
                     VFGESDELPGLIVDRYGDIAVGQIATAGMEARRPAIEAAIREVVAPRTLVWKNDTGAR
                     DLEQLPKEITVAFGELPEELAVIENGIEFAVPFAQAQKTGWFYDQAANRARLRELLPR
                     DARVLDVCSYAGAWAVTALKSGARAAACVDASAGALQAAGRNAARHDHALETLQGDAF
                     DVLESLAAAGRRFDAIVLDPPAFAKRRKDLPKAQAAYRKLNQLALRLLDEEGLLVSCS
                     CSWHLAESDLLAAIQAAARHVSRFVQVLAVGGQAPDHPLHPAIPETRYLKAFFCRVLS
                     GQAPTT"
     gene            5976..8042
                     /locus_tag="PKN91_07625"
     CDS             5976..8042
                     /locus_tag="PKN91_07625"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNS27978.1"
                     /translation="MSNALSGETSPYLLQHADNPVEWYPWGEQALALARESGKPILLS
                     VGYSACHWCHVMAHESFEDPDTARLMNELYVNVKVDREERPDIDRIYQIAQQMLTQRS
                     GGWPLTMFLAHDDQRPFFGGTYFPPEARHGMPAFRDLLVRVAEYYREHRDELGRQNDA
                     LMEAFATLESPPADAAAPLTDAPLLACRSQLERSFDRVNGGFGGAPKFPHPTTIDRLM
                     RDWHATAGSPEPDLHALYMATLTLTRMAEGGLFDHLAGGFYRYCVDAQWSIPHFEKML
                     YDNGALLASYASAALATGEVLFARTAGQTAAWLLADMRSPEGGFYSSRDADSEGHEGR
                     YYVWTREAVESALTAAEFAVFAAHYGLDREANFEGAWHLRVTRSLEDIAAGLGSTRDA
                     VAAQIDGARAKLLALRERRIPPARDEKLLTSWNAIAIRGLAMAARALDQPALAEAATG
                     AVDFLRARLWRDGRLLATYKDGRAHLNAYLDDYALLADALIELLQVRWRGADLEFAVA
                     LLEAMLEHFEDREAGGFFFTSHDHERLIHRSKSFADDATPCGNGVAATVLQRAGYLLG
                     EPRYLAAAERTLRAGWSGVSRQPQAHTSMLVALEEYLAQPQIVILRGTAAGIERWRHE
                     LARLHAPRRITLAIPDDAPGLPAALAAKAPRGDAVAYLCRGSTCSAPIDSLSVLLRDL
                     RLALAS"
     gene            complement(7999..8481)
                     /locus_tag="PKN91_07630"
     CDS             complement(7999..8481)
                     /locus_tag="PKN91_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HNS27979.1"
                     /translation="MHKTILASIMGLALCVTAVAGDGQREYIGAAAGSVAATLPFSNG
                     VRVGDTLYIAGHLGLDAETQMPPAGVEDEARLVMDGIKATVEAAGFTMNDLVSVTVFC
                     PDLTLYEAFNRVYRTYFSGRFPARAFVGSGPLVRGARFEVLGTAVRTPAPAAGHAAAH
                     "
     gene            complement(8495..8908)
                     /locus_tag="PKN91_07635"
     CDS             complement(8495..8908)
                     /locus_tag="PKN91_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27980.1"
                     /translation="MDAARRGDEPRVLARLGSGWAVFGSQQFVRGYLLLLPDPVVPQL
                     NALAGAARAGFLADMTRLGDALLQVTDAVRINYAIFGNVEPALHAHVIPRYAHEPAAM
                     RTAQPWAYDWSRAEPFDAAALAPLAEALRQALRKL"
     gene            complement(8941..9222)
                     /locus_tag="PKN91_07640"
     CDS             complement(8941..9222)
                     /locus_tag="PKN91_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNS27981.1"
                     /translation="MPAILPATGLLILSNAFMTFAWYAHLKNFSTRPWFIAALVSWGI
                     AFFEYMLQEVIALAVFVPFAVLYMREPLKLDYLWASLCILGAVYFMFRS"
     gene            complement(9294..9860)
                     /locus_tag="PKN91_07645"
     CDS             complement(9294..9860)
                     /locus_tag="PKN91_07645"
                     /inference="COORDINATES: protein motif:HMM:NF017736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HNS27982.1"
                     /translation="MSYEFQWSRRRMLAATLATAVAAPAILHTRPAPAAEMIGPQRWL
                     ELFNTHTGETVSVAYHDGRRVVADAVAKLERVLRDHRANEVHPIDPDLYDQLSAVALE
                     AGRDARFEVISGYRSPHSNAKLAAASSGVARRSLHMQGRAIDVRLKGASCASVRDIAL
                     AMARGGVGFYRKSNFVHLDTGRVRSWQG"
     gene            complement(9971..11152)
                     /locus_tag="PKN91_07650"
     CDS             complement(9971..11152)
                     /locus_tag="PKN91_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HNS27983.1"
                     /translation="MTTPRRARARPRWIDPFAPEHIRRLVAEFGSPLLIVDCARVRAQ
                     YHNLRRALPGVDLHYALKPLPHPAVVQTIIEEGGFLDLATTGELQLVANLGVDPARCI
                     HTHPIKRDIDIRNAVHAGVRVFVADNPDELEKFVPWRDTTELLLRVSFRAPGAVCDLS
                     RKFGCDPEALLPLARRARELGLTVRGLSFHVGSQAPNALKHLEAIETCSKLLRAARRE
                     KLGPLDTLDIGGGFPIGYGQRAPDIGRFCSSLRAAIVKLPRRVRVIAEPGRYIAGPAA
                     IGVASVMGRAQREGHWWYYLDDGLYGSYSGQLYDHARYAVEALREGTERLPSVLAGPT
                     CDSIDVIAENLMLPRLREGDLIVGRSMGAYTWASASEFNFFPRATVVAVNRTAGDMGG
                     V"
     gene            11224..12654
                     /locus_tag="PKN91_07655"
     CDS             11224..12654
                     /locus_tag="PKN91_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS27984.1"
                     /translation="MQTHAATDPHAELRTLLSSRVALLAVESRDETRALALLRDAGLH
                     ARRAPGSGIFIWSATEGLKRIDRDMGAPQRHLIEPAQMLRHLKSTPMAGIYILLDFHP
                     YLADPVNIRMLKDVVQDQAKVARTVVLVSHEITLPAELVPLAARFRPALPSAAERQKI
                     VTHVFDEWRRQHKGRAPRIDRDALARVIDNLAGLSAPDAERLVRRVLYDDGAITAEDL
                     RAVQAGKYELLNRHGTLGFEPDTARFAEVGGLANLRRWVETRRAAFDGTAPGLDAPRG
                     VLLLGVQGCGKSLAARAIAGIFGVPLLRFDFAALYSKWHGESEQNLRETLATATALAP
                     CVLWIDEIEKALASGEGDSGTGRRVLGAFLTWMAERRTRVFLVATANDVSALPPELIR
                     KGRFDEIFFVDLPDAETRAAILGIHAARRNVPLTGAELARLAAMSGEFSGAELEQAIV
                     AALYTAHAQGAPVDAASTGAPCACAV"
BASE COUNT         2047 a         4431 c         4301 g         1882 t
ORIGIN      
        1 gcgggcggct ggtacacgat cacgacggcg gcgagcagtt gctgccctat gggcagcacg
       61 acctcgaggt catccattcg gtgtcgcgcg aggctctcgg gcgcacgctg gtcgaggcag
      121 cggcggagca gcgcggcatc gccctgcatt tcgggcagac ctgtgtcggc gtcgacgtgg
      181 cgcgcgatgc cctgctgttc ggcacggcgg acaaggtgca gcggcgcgtg ccgctcgtgc
      241 cgaccatcgc gaccgacggt gcgggttcgg ccgtgcgcgt cgcgctcgcg gcagccggcg
      301 ccatcaccgt gcgcgaggac atgctggcgc acgactaccg ggagctcacc gtgccgccgg
      361 agcgggcccg tggactgcac ccgaacgcat tgcacatctg gccgcgtggc ggcttcatgc
      421 tgatcgcatt gccgaacacc gatggcagct tcaccgccac gctgttcctc gcgcgcgagg
      481 ggtcgccgtc cttcgcggag ctgacggacg ccgcggccat ccgcgatttc ttcgcgcgtg
      541 aattcccggg cgccgtgccg ctgctgccgc gcctcgtcga ggagttcgcg gaaaaccccc
      601 agggccggct cggcaccgtc tacagcgaac cctggcatgt cggcggcaag gtgctgctgc
      661 tgggtgacgc cgcgcatgcg atcgtgccgt tccacggcca ggggatgaac tgcgcattcg
      721 aggacgtcgc ggtactcgat gcgctgctcg gcgaacacca ggacttcgac gcactgttcg
      781 gcgccttcat gcgtgcgcgc cgccccgaca ccgacgcaat cgcacagatg gcgctcgaga
      841 attacctcga gatgcgcgac acggtgctcg accctgcgtt ccagcgccgc aagacactgg
      901 gttcctgcct ggagcgccac tatcccgagc ggttcattcc gcgctattcg atggtgatgt
      961 tccatccgga gatcggttac gcggaggcgc tgcggcgcgg cgccgtccag gatgcactgc
     1021 tcacccgcat cgacgccgcg catcctggcg ccgaccctga ggagatcgcc gacctggccg
     1081 ccgtacggga actggtcgag gcgcgcctgc cgcgcctcaa tcgcgcgagc gccgctcgaa
     1141 cgtgacgaag tccatcgcat gggcgtgccg cgcgtcggcg gcatggtgct cgcgcgccac
     1201 ttcgcgccac tcgccccgat cgaagggcgg caggcgggtg tcgccctcca cttcggcgtc
     1261 gatggccgtc aggtgcaccc ggaacgccag cggcgcagcg agcgcgtaaa cctgcgcgcc
     1321 gccgatcacc atcaactcct cgtggcccgc cgccgccgcg actgcagcct cgagtgaggc
     1381 cacggcgttc gcgccctcgg cacgaaacgc cgggtcgcgg gtcaacacca ggttggtgcg
     1441 gcccggcagc ggcctgccga tcgattccca ggttcgccga cccatcagga tcggcttgcc
     1501 cagcgtcagc gccttgaagc ggcgcaagtc gtcgggcaga tgccagggca gcgcgccgtc
     1561 ctggccgatg acgccattgc gcgccatggc cacgaccagc acgatgcgcg gcgcgccgtt
     1621 caccgccgcg cgcgcttctt cgcccccacc ttggacttcg taccggtctt gcgcttcgcg
     1681 ccggtcttgc gcttcgcgcc ggtcttgcgc ttcgcgcgct tcttcggccg cttggccggc
     1741 cacagcatcg tgccgcggca ggtcgcggcg ccgcagcggc acgcaaagat ctcatcgacg
     1801 ttcggcgggt cgctgtcggc gcggccgatc tggtaatcgt agttcagctc ctcgcccggc
     1861 cggatcgtgc ggatcgcttc gatgaacacg cgccggttct cgatcaccga ctcgcagttc
     1921 gggtcgcagc catggttgat gaagcgcgca tcgttgccgt cgacgcccgc atcgatcacg
     1981 acgccgcgat cgacgatgaa gaggaaagtg tggccgtcgt cctcgtcctt gtcctcgtag
     2041 cgctggtcgg cgaccttgtg cgaaatgcgg tcaccgagat actcgatgat ccgcgtgccc
     2101 ttgcggatgg ttcgggtcgc gaacacgccg aagccgtgga tcttcgagcg ccgtaccttg
     2161 tacatcgggg acttgttcgt ggctgacatg cacttccttc ttcagatggc caccggcgcc
     2221 ttgatcgccg gctgcggttg ataattgcgg aactcgaaat cctcgaaccg gtagtcgaac
     2281 aatgtctccg gacgtcgcgc gatcacgagt tcgggcagcg gcagcggctc gcgtgcgagc
     2341 tgcgtgtccg cctgctcgag atgattcagg taaaggtgcg tatcgccgcc cgtccagatg
     2401 aactcgccga cgccgagatc gcactgctgc gcgatcatgt gggtcagcag cgagtaggag
     2461 gcgatgttga acggcacgcc gaggaacagg tcggcgcttc tctggtagag ctggcacgag
     2521 aggcgcccgt ccgccacgta gaactggaac agcgcatggc acggcagcag ggccatgcgg
     2581 tccagctcgc cgacattcca ggcgctgacc acgatgcggc gcgaatgcgg attcgtgcgc
     2641 aattccccga gcacccgcgc gatctggtcg atgtgccctc cgtcgggcgt cggccatgaa
     2701 cgccactggc tgccgtatac agggccgagt tcgcccgcgg catcggccca ttcgtcccag
     2761 atcgtgaccc cgtgctcgcg caggtaggcg acgttggtgt cgccgcgcag gaaccacagc
     2821 aactcgtaca cgaccgattt cacgtgcacg cgcttggtcg tcaacagcgg gaaaccccgt
     2881 gtcaggtcga agcgcatctg gcagccgaac agcgagcgcg tgcccgtgcc ggtacggtcg
     2941 ggcttgtgcg caccgtggtc gcgcacgcgg cgcaggaggt cgaggtaagt ctgcattctg
     3001 aacggcgatt ataggcatgc gcgcgcagtg attcagactc ataatagggg tcgaccaccc
     3061 caccacgcag gacaccgtca tgttcactcg caatgccgtc cccgcgctcc tttccggttg
     3121 cacgctggcg ctgctcctcg ccgcttgcgg cagccccgcg ccatcgaacg acgccgccac
     3181 cgcggccgaa gatgccgccg cggcgctcgc agagaaggaa gcggcgctcg ccgagcgcga
     3241 agccgcgttg gcggaaaagg aagccgaaca gcaggaacag gaagccgcgc gactggctgc
     3301 cgaagaggcc gcccggctcg agagcgaagc tgcggccgca gccgcggccg cggcggctgc
     3361 caagaagccg gcgccggcca agacttcgac ggcggcgagc aagcctgcca cgagtgcgcc
     3421 ggcctcgagc acggcggcaa agccggccgc gccgccgccg ccgctggtcg taccggcagg
     3481 taccaccctc accgtcgcgc tcgcacaggc gttgagcacc aagaccgcca aggtcggcga
     3541 tcccatcagt gcacaagtcg tctccgacgt catggttggc aaccgtgtcg cgattcccgc
     3601 aggttcgcgc gtctccggga ccgtgaccaa cgtggtctcg ggcagcagca agatcggcgg
     3661 cgtgcccgcg ctcggcctgc gcttcgatac gctgcagctg atcggcggac agagcatgcc
     3721 gatcagcggc gagatcgtgc agcaggggcg cagcgatacc ggcatggaca ccgcgaagat
     3781 cctgggtggc gccgcggccg gtgccgtcat cgggcaccag gtcgacgacg acaagggcaa
     3841 ggtcatcggt ggcctgctcg gcggcgcggc cggcgcgatt gcggccaaga agaccggtgc
     3901 cgaggtgcag ctcgcacagg actccacgct ggcgatcgcg ctcggcgcgc cgatcgaggt
     3961 ggcggggcgc tagcgcgcgt agttgcccga aggctcgcgc cgggcgtagg cccacgcgag
     4021 catggcgacc cccgcgacca gcagaggcag cgacagcagc tggcccatcg tgagccagtt
     4081 gccggcgaag tagccgatgt gcgcgtcggg cacgcgccag aactcgacca gcatgcgcga
     4141 gacggcatag atcacgagga acaggcccgt cggcgcaagc cgcgggcgcg gcttcgacgt
     4201 gaaccaccag atcacggcga acagcagcag cccctccagc gccgcctcat aaagctgcgt
     4261 cgggtggcgc acgacgccgt cgacctcgaa gccccacggc accgtggttt cgctgcccca
     4321 cagttcgccg ttgatgaaat tgccgatgcg gcctgcgaac agtccaatgc ccggcagcgg
     4381 cgcgatgaaa tcatagacgt cggcggtgcg gcgccgccgc cgccatgcaa aaagccacag
     4441 cgccaccgcg acgccggcca ggccgccatg gaacgacatg cccccctccc agatcttgag
     4501 gggatacagc ggatcgaggg cccagtagcc gagcccgtag aacagcacat agccgacgcg
     4561 accgccgatg atcacgccga gcatcgcgaa gaagatcagg tcatcgacgt cctgaggctt
     4621 ccaggtcgag ccgggccgag ccgcacggcg ccggcccagc caccagccgg cgacgaatcc
     4681 gacgaggtac atgatgccgt accagtggat cctgaccggg ccgagctgca acgcgatcgg
     4741 atcgatgccg ggatacttga tcatgtggtg ggtgcctggc cgctcagcac gcggcagaag
     4801 aaagccttga ggtaacgggt ttcgggaatc gccggatgca gcggatgatc gggcgcctgc
     4861 ccgccgaccg cgagcacctg cacgaagcgc gacacatggc gcgccgccgc ctggatcgcc
     4921 gccagcagat cgctctcggc gagatgccac gaacacgagc acgacaccag caggccttcc
     4981 tcgtcgagca ggcgcagcgc cagctggttc agcttgcgat aggccgcctg cgccttcggc
     5041 aggtccttgc gacgcttggc gaaggctggc ggatcgagca cgatcgcatc gaaacgccgg
     5101 cccgcggcgg cgagcgattc gagcacgtcg aaggcgtcgc cctgcagcgt ctccagggca
     5161 tgatcgtgcc gcgcggcatt gcggccggcc gcctgcagcg cccccgcgga cgcatcgacg
     5221 caggccgccg cacgcgcccc ggacttcagt gcggtgaccg cccaggcgcc cgcatacgag
     5281 cacacatcga gcacgcgcgc atcgcgcggc agcagctcgc gcaggcgggc acgattggcc
     5341 gcctggtcgt agaaccagcc ggtcttctgc gcctgggcga aaggcacggc gaactcaatc
     5401 ccgttctcga tgacggcgag ctcctccggc agctcgccga acgccacggt gatctccttc
     5461 ggcagctgct cgaggtcgcg cgcacccgtg tcgttcttcc agacgagcgt gcgcggcgcg
     5521 accacttcgc ggatcgccgc ctcgattgcg ggcctgcgcg cctccatgcc ggcggtcgca
     5581 atctgcccca ccgcgatgtc gccgtagcgg tcgacgatga gccccggcag ctcgtcggac
     5641 tcgccgaaca cgagccggta gtgcggcgtg ggatggagcc gctcgcgcag ccgcagcgcg
     5701 cgtgcgatgc gcgaggcaag cagcgcgccg tcgggctcgc gccgttcgtc ccgcgacagc
     5761 aaccgcgcgc tgatcagtgc gtgcggattc acgtagccat agccgagaaa cgcgtcgcga
     5821 tccgacacga tgcgcgcggt gagatcgggg gtgaatgcgg acagcggcgt cgcgccggta
     5881 tcgacctcgt tgctgaaaac ccagctgtgg ccggcgcgaa gcctgcgatc ctcgcccttt
     5941 ttcaagcgca acagcattcg cgtattctag ccgacatgtc gaacgccctg tccggtgaaa
     6001 cgagcccata ccttctgcag cacgccgaca atcccgtcga atggtacccg tggggcgagc
     6061 aggcgctggc gctggcccgc gagagcggca agccgatcct gctctcggtg ggctattcgg
     6121 cctgccactg gtgccacgtg atggcgcacg aatctttcga ggatccggac accgcgcggc
     6181 tgatgaacga gctgtatgtc aacgtgaagg tggaccggga ggaacgcccg gacatcgacc
     6241 gcatctacca gatcgcccag cagatgctga cccagcgcag cggcggctgg ccgctgacga
     6301 tgttcctcgc gcacgatgac cagcgcccgt tcttcggcgg cacctatttc ccgccggaag
     6361 cgcgccacgg gatgccggcc ttccgcgatc tgctggtgcg cgtcgcggaa tattaccgcg
     6421 agcatcgtga cgagcttggc aggcagaacg atgcgctgat ggaagccttc gccacgctcg
     6481 aatcgccgcc ggccgatgcg gcggcaccgc tcaccgatgc gcccctgctc gcctgccgca
     6541 gccagctcga gcgctcgttc gaccgggtga acggcggctt cggtggcgcg ccgaaattcc
     6601 cccacccgac cacgatcgat cgcctgatgc gcgactggca cgcgactgcg ggctcgcccg
     6661 agcccgacct gcatgcgctc tacatggcga cgctcacgct gacccgcatg gcggaaggcg
     6721 gcctcttcga tcatctcgcg ggcggcttct accgttactg cgtcgatgcg cagtggtcga
     6781 ttccgcactt cgagaagatg ctgtacgaca acggcgcgct gctcgcgagc tatgccagcg
     6841 cggcactcgc caccggcgag gtcctgtttg cgcgtaccgc cggacagacg gccgcgtggc
     6901 tgctcgccga catgcgctca ccggagggcg gtttctactc gagccgcgat gcggactccg
     6961 aggggcacga ggggcgctac tacgtgtgga cgcgggaggc ggtcgaatcc gcgctgaccg
     7021 ccgccgagtt cgcggtgttt gccgcgcact atgggctcga ccgcgaggcg aacttcgaag
     7081 gcgcgtggca cctgcgcgtc acgcgcagcc tcgaggacat cgccgccggg ctcggcagca
     7141 cccgcgacgc ggttgccgcg cagatcgacg gcgcccgcgc gaagctgctg gcgctgcgcg
     7201 aacggcgcat accgccggcg cgtgacgaga agctcctcac cagctggaat gcgatcgcga
     7261 tccggggcct ggccatggct gcccgggcac tggaccagcc ggcgctcgcc gaggccgcca
     7321 cgggcgcggt cgatttcctg cgtgcacgcc tgtggcgcga tgggcgcctg ctcgcgacgt
     7381 acaaggacgg ccgcgcgcac ctcaacgcct atctcgacga ctatgccctg ctcgccgacg
     7441 ccctcatcga gctgctgcag gtacggtggc gcggcgcgga cctcgagttc gccgtcgcac
     7501 tgctcgaggc catgctcgag cacttcgagg atcgcgaggc cggcggtttc ttcttcacct
     7561 cgcacgacca cgagcgtctg atccaccgct cgaagagctt cgccgacgat gcgacacctt
     7621 gcggcaacgg cgtcgctgcc acggtattgc agcgcgcagg ctacctgctc ggcgaaccgc
     7681 gctatctcgc ggcggcggaa cgcacgctgc gcgcgggctg gagcggcgtg tcccgccagc
     7741 cgcaggcaca tacatcgatg ctggtggcgc tcgaggagta cctcgcgcag ccgcagatcg
     7801 tgatcctgcg cggcacggcc gccggaatcg agcgctggcg gcatgaactc gcgcggctcc
     7861 atgcgccgcg ccgtatcacg ctcgcgatcc cggatgacgc cccgggtctg ccggccgcac
     7921 tggccgcgaa agcaccgcgc ggcgatgccg tggcctacct gtgccgcggc agcacctgca
     7981 gtgcgccgat cgactcgctc agtgtgctgc tgcgtgacct gcggctggcg ctggcgtcct
     8041 gaccgccgta ccgagcactt cgaaacgcgc gccgcgaacc agcgggccgc tgccgacgaa
     8101 tgcgcgcgcc gggaacctgc cgctgaagta ggtgcggtac acgcgattga acgcctcata
     8161 cagcgtgagg tcggggcaga acacggtcac cgaaacaagg tcgttcatgg tgaacccggc
     8221 ggcctcgacc gtggccttga tgccgtccat gacgaggcgg gcctcgtcct cgactccggc
     8281 cggcggcatc tgcgtctccg catcgaggcc gaggtggccg gcgatgtaca gcgtatcgcc
     8341 gacacgcacg ccgttgctga acggcagcgt cgcggcaacc gagccggctg ccgcgccgat
     8401 gtactcgcgc tggccgtcgc ccgccaccgc ggtaacgcag agggccaggc ccatgatgct
     8461 tgccaggatc gtcttgtgca tgtccacttc tccgctagag tttccgcagt gcctgccgca
     8521 gcgcctcggc gagcggcgcc agcgccgccg cgtcgaaggg ctccgcccgc gaccagtcgt
     8581 aggcccaggg ctgggcggta cgcatcgccg cgggctcgtg cgcgtagcgc gggatcacgt
     8641 gcgcatgcag ggccggctcg acgttgccga atatcgcata gttgatgcgc acggcgtcgg
     8701 tcacctgcag caatgcatcc ccgaggcggg tcatgtccgc gaggaacccc gcccgcgccg
     8761 ctccggcgag cgcgttcagc tgcggcacca cgggatcggg cagcagcagc aggtagccgc
     8821 gcacgaactg ctgcgagccg aacacggccc agccggaacc gagccgcgcg agcacgcgcg
     8881 gttcatcacc ccggcgtgcg gcgtccacat cgcgatggat tgcggtgtcc gcccgcggca
     8941 tcacgagcgg aacatgaagt agacggcgcc gaggatgcac agcgacgccc acaggtagtc
     9001 gagcttcagc ggctcacgca tgtagagcac ggcaaacggc acgaagacgg cgagcgcgat
     9061 cacttcctgc agcatgtact cgaagaacgc gatgccccag ctcacgagtg ccgcgatgaa
     9121 ccatgggcgg gtcgagaagt tcttgaggtg cgcgtaccac gcaaaggtca tgaacgcgtt
     9181 ggagagtatc agcaggccgg tggccggcag gatcgcaggc atgtatcgcc cccgtagatc
     9241 gcccattctc gcgggcggga ggcggtcatg caatcagggc ggggtgctgc cgatcagccc
     9301 tgccagctgc gtacgcgacc ggtatcgagg tgaacgaagt tcgacttgcg gtagaagccg
     9361 accccgccac gcgccatcgc gagcgcgatg tcccgtacgc tggcgcacga ggcgcccttc
     9421 aggcgcacgt cgatcgcgcg gccctgcatg tgcaggctgc gcctcgccac gcccgaactc
     9481 gccgcggcaa gcttcgcgtt gctgtgcggg gagcggtacc cggagatcac ctcgaagcgc
     9541 gcatcccggc ccgcctcgag cgccacggcg gacagctggt cgtagagatc ggggtcgatc
     9601 ggatgcactt cattggcccg atgatcacgc agcacacgct cgagcttcgc caccgcgtcc
     9661 gccaccacgc ggcgcccatc gtggtaggcg acgctcacgg tctcgccggt atgggtattg
     9721 aagagttcga gccagcgctg cgggccgatc atctcggccg ccggcgcagg gcgcgtgtgc
     9781 aggatggccg gcgcggccac cgcggtcgcc agcgtggcgg ccagcatgcg cctgcgcgac
     9841 cattgaaatt cgtacgacat aatcagcttc ccgcccggcc aaggtcttcc ggaatcagtc
     9901 caacccggat ggttcaggac tcgggcgcgt aatgcaagca ggcgtcgggg aaaccccgag
     9961 ctgcctcgca tcagacgccc cccatgtccc ccgccgtgcg gttcacggca accactgtgg
    10021 cgcgcggaaa gaagttgaac tccgaggcgc tcgcccaggt gtacgcgccc atcgagcgcc
    10081 cgacgatgag gtcgccctcc cgcagtcgcg gcagcatcag gttctcggcg atcacgtcga
    10141 tgctgtcgca ggtcggaccc gcgagcaccg agggcagacg ctcagtgccc tcgcgcaggg
    10201 cctcgaccgc atagcgggca tggtcgtaga gctggccgct gtaggagccg tagaggccat
    10261 cgtccaggta gtaccaccaa tgcccctcgc gctgggcgcg gcccatgaca ctcgcgacgc
    10321 cgatcgccgc tggtccggca atgtagcgac ccggctcggc gatcacgcgc acgcggcgcg
    10381 gcagcttcac gatcgcggcg cgcagtgatg agcagaagcg gccgatgtcg ggcgcgcgct
    10441 gtccgtagcc gatcgggaat ccgccgccga tgtcgagcgt gtccagcgga ccgagtttct
    10501 cgcggcgcgc ggcccgcagc agcttgctgc aggtctcgat cgcctcgaga tgcttcagcg
    10561 cattcggcgc ctgcgagccg acatgaaacg acaggccccg cacggtgagc ccgagttccc
    10621 gcgcccgccg cgcaagtggg agcagtgcct ccggatcgca gccgaatttg cgcgacaggt
    10681 cgcaaacggc acccggcgcg cgaaacgaca cccgtagcag cagctcggtg gtatcgcgcc
    10741 aggggacgaa cttctccagt tcatccggat tgtcggcgac gaacacgcgc acgccggcgt
    10801 gcacggcatt gcggatgtcg atgtcacgct tgatcgggtg cgtgtggatg caccgcgccg
    10861 ggtcgactcc gaggttcgcg acgagctgca gctcgccggt cgttgcgagg tcgaggaatc
    10921 cgccctcttc gatgatggtc tgcacgaccg ccgggtgcgg caggggcttc agcgcgtagt
    10981 gcaggtcgac accgggcagc gcccggcgca ggttgtggta ctgcgcgcgc acgcgcgcgc
    11041 agtcgacgat caggagcggc gagccgaatt cggccaccag acgtcggatg tgctcggggg
    11101 caaacgggtc gatccagcgg ggcctggcgc gggcgcggcg cggggttgtc atttgtgacg
    11161 cattgtcggg aatgaccgca gggcccgcaa gatcggcctg ctcatttgct acattgttcg
    11221 tccatgcaga cgcacgccgc cacggacccg cacgccgagc tgcggacgct gctctcctcc
    11281 cgcgtcgccc tgctcgcggt ggagagccgc gacgagacgc gtgcgctggc gctgctgcgc
    11341 gacgccgggc tgcacgcgcg ccgcgcgccc ggatcgggaa tcttcatctg gagcgcgacc
    11401 gagggcctca agcgcatcga ccgcgacatg ggcgcgcccc agcgccacct gatcgagccg
    11461 gcgcagatgc tgcgccacct caagtcgacc cccatggcgg gcatctacat cctgctcgac
    11521 ttccatccct atctcgccga tccggtgaac atccgcatgc tgaaggatgt cgtgcaggac
    11581 caggcgaagg tggcccgcac cgtagtgctc gtcagccacg agatcacgct tccggccgag
    11641 ctcgtgccgc tcgccgcgcg ctttcgtccc gcgctgccct ccgccgccga gcggcagaag
    11701 atcgtcacgc acgtgttcga tgaatggcgg cgccagcaca agggtcgcgc gccacgcatc
    11761 gaccgcgacg cgcttgcgcg ggtcatcgac aatctcgcgg gactcagcgc cccggacgcc
    11821 gagcggctcg tacggcgcgt tctgtacgac gacggcgcga ttaccgccga ggacctgcgc
    11881 gccgtgcagg ccggcaagta cgaactgctc aatcgccatg gcacgctcgg cttcgaaccc
    11941 gacaccgcgc gtttcgccga agtcggcgga ctggccaacc tgcgccgctg ggtcgagacg
    12001 cgtcgcgcgg ccttcgacgg caccgcgccc ggcctcgatg ccccgcgcgg cgtgctgctg
    12061 ctcggcgtgc aggggtgcgg caagagtctc gcggcccgcg cgattgcggg cattttcggc
    12121 gtaccgctgc tgcgcttcga cttcgcggcg ctgtacagca agtggcacgg cgagtccgag
    12181 cagaacctgc gcgaaaccct cgccaccgcg accgcactcg caccctgcgt gctctggatc
    12241 gacgagatcg agaaggcgct cgcctccggc gagggtgaca gcggcacggg caggcgcgtg
    12301 ctcggcgcgt tcctcacctg gatggcggag cggcgcacgc gcgtgttcct cgtcgcgacc
    12361 gccaacgatg tcagcgcgct gccccccgag ctgatccgca aggggcgctt cgacgaaatc
    12421 ttcttcgtcg acctgccgga cgccgaaaca cgcgccgcga tccttggcat ccacgccgca
    12481 cggcgcaacg taccactgac cggcgccgag ctcgcgcgac tcgccgcgat gagcggggag
    12541 ttctcgggcg cagagctcga gcaggccatc gtcgcggcgc tctatacggc gcacgcgcag
    12601 ggcgcgccgg tcgatgccgc atcgaccggc gcgccctgcg cgtgcgccgt atagagcgcc
    12661 g
//
LOCUS       DAOQVY010000068        12353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_5814_length_12353_cov_5.187591, whole genome shotgun sequence.
ACCESSION   DAOQVY010000068 DAOQVY010000000
VERSION     DAOQVY010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12353
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5814_length_12353_cov_5.187591"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKN91_07660"
     CDS             <1..311
                     /locus_tag="PKN91_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNS27985.1"
                     /translation="DIYQREGLLTRAKTLSKTWEDAVHGLRGRPHVIDTRNYGLIAGI
                     EFEAIPGQAGARAFEIFRKCFERGLLVRTTGDILALSPPLIIEPAHIDEIFGILADVL
                     "
     gene            complement(357..1067)
                     /locus_tag="PKN91_07665"
     CDS             complement(357..1067)
                     /locus_tag="PKN91_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HNS27986.1"
                     /translation="MNRFKRTSLLLGLLAVAGTAHAQFSATITATSDYDFRGVSLSAK
                     DPALQASLDYAFGETGFYVGAWGSNIDYGDDVDGDIEVDLYAGYDGAINDDTGWTVGL
                     TWYTYPGSSNSATRSKIEAYPEAWLGINYKGLDLKQWYAFEYDGTNDALYTEANYTFE
                     LPQNFSLSLHAGYNYGDAFEDFEYFDYSVAVGYELGNFGLELKYTGTDLSGDDKVTDD
                     VFNTEGRLVFSVSTTLPW"
     gene            complement(1165..1521)
                     /locus_tag="PKN91_07670"
     CDS             complement(1165..1521)
                     /locus_tag="PKN91_07670"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNS27987.1"
                     /translation="MAMEIIPVTLAGEGEASRPAADRLVSGDPVQHAWNAYSDPAECF
                     HVGHWSSTRGVWRVRYTESELCVLTAGRVELVSDSGTRMCFAKGDAFVVPSGFSGTWA
                     VLEDCTKIYAIYIPRA"
     gene            complement(1521..2765)
                     /locus_tag="PKN91_07675"
     CDS             complement(1521..2765)
                     /locus_tag="PKN91_07675"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNS27988.1"
                     /translation="MPWLFRDPQGAAAEWRARLALLLADLRADARACQAGIADAPGAL
                     TRQRLELLAGALDDHAQRLASLLEPLGVDADPSAREALQALRTRLPGSQGLTNYYANI
                     HRDWCWGEAENEATLALLAAVADGDLAGARIAVLGSGAGRLAYDLYSSFAPALVVAMD
                     LNPLLLAVAREMYAGRRLELYELPIAPRSIADHAVLRRLHAPAAADRRLVLVAGDALQ
                     APFAPGAFDVVVTPWFIDIVEAPFPALAARVNALLKTGGRWLNLGSVAFASAQRAQQF
                     SLEETLQLAEEAGFDPPRTREEPLPYMRSPASRHARVESTVAWSARKRAEVPAPAAHE
                     PPVWLADVTQPVPLPAELRSHALATRIHAFLMSLVDGHRSVADIARVLVEQKLMRPED
                     ATPAVRAFLARMYEDLGRRTNF"
     gene            2912..3700
                     /locus_tag="PKN91_07680"
     CDS             2912..3700
                     /locus_tag="PKN91_07680"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNS27989.1"
                     /translation="MTRQRGRPKANGLALHHRLYMMLKQQLADGTIAPGAALPSEPDL
                     ALSTKLSRTTVRRALARLAAEGLIERRHGSGTYATMQGAARAKAVASADYGRLLGDLR
                     TMARVTKARLLQYAVIATPPRILAVNPELGTRVLYMQRTRLYRGKPFVVISHYIPERL
                     VKLLPKAQLKSVPPIFLFEKKGMVPTIGDQEVTAVAADHVFSRELAVNIGAPLLAIRR
                     SVRDSRGTLLEYHEAFYDPELYSVQMQVRRERVGKSGMRWRPGR"
     gene            complement(3703..6285)
                     /locus_tag="PKN91_07685"
     CDS             complement(3703..6285)
                     /locus_tag="PKN91_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS27990.1"
                     /translation="MSVDDAEQELFDACVEADEATRRRLLADCPDPALRERVRRLLSA
                     HDEAPDSFLGGAPPEIPRLAAPLQIGPYRVIERIGEGAMGEVFLAEQRAPMRRNVALK
                     ILKFGLATREVLARFELERQALAMLTHPNIARIYDSGTTGDGRPYFAMEYVAGTAVTR
                     YCEDHGLAVESRLELFGEICAGVQHAHLRGIIHRDLKPSNILVTEIDGRPTPRIIDFG
                     IAKAAMTDGGDADAHTRVGHLLGTPEYMSPEQAQLAPLDVDARTDVYSLGVILFELLA
                     GVRPYDVRGSEYNPVALAGEIANREAVRPSAVAVTPARAARLRGDLDWIVMKALAPDR
                     HLRYSSPAELAADLERHANNDTVLAGPPTARYRIGKFVRRHRLAVTAAASLFVAALGF
                     GSGMAWLARQAALERDRANAEAATARRIVAFTVGLFEIANPAQSGAREITARELLDAG
                     VQRLEAQPDDGRADVRAALLEAAGNAYSGLGAYDAAGKLLARAVVLRESAVEREPQAY
                     AQALASQAALKREQGDWVASESLAREALAVVTAVPDGDLAVRETVRLELADTLRRRSQ
                     LDEAAQLALQSARAFEAADQGGTLGYVRARFLLGRIRAAQGDLAEAERLLSDALAQGR
                     AIGGDLDALTLDMRSGLAEVLVNRGDIERAEPLLRDFVRDIERIYGRGHPHAGIARNN
                     LGNALSDMPSRVGEAESVYQEALAILLAAFGERHPEVATVHNNLGSLYLLTGRWADAE
                     AALARAIEIRESTLGADHPDTGAARTGRALALNKLGRYTEAEVLLRASMRDMRAALGD
                     GHWRAANAQRYLATVLTNRGRYEEAEAELRAALEVFRRELGAEHARTVSAAKELDALV
                     ARRR"
     gene            complement(6282..6842)
                     /locus_tag="PKN91_07690"
     CDS             complement(6282..6842)
                     /locus_tag="PKN91_07690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNS27991.1"
                     /translation="MAHTDVTLLLEAAGSGDRQAFERLYRVVYDELHRMARANMRREG
                     RADTLQPTALINEAFLRLAPNGAVWQNRRHFFGAAAEAMRRVLVEQARRRHAQKRGSD
                     AVRVTLTGLEIPIPEDDADVLLVDAALTELAAGRPRLAELVTLRFFAGLSIEEAAEAL
                     GISVATAKRDWVYARAWLQERLSAGA"
     gene            6906..7859
                     /locus_tag="PKN91_07695"
     CDS             6906..7859
                     /locus_tag="PKN91_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS27992.1"
                     /translation="MNAVVAERVPDAGRAARYARCVNMSKKVEWEIERDLLRGRDFDF
                     SRKFLPDGLSLVERLSLAPGEARLMSQVQGRTYAYIFGLVERFISAGMVDAAGKHAFG
                     NQVAMEGLLRFSGEELKHQELFRRMEQMMAQAMPAGYSQVADPNDVARAVLERRRWAV
                     LALTCHIELFVQAHYEQSIAAEADICPLFRDVFLYHWKDECQHVMLDELEWTAEHARL
                     TPAEHDAAVDDLIALVGAVDGLLQGQSRSDAAYFMSAAGREFPAAEAAQIEAAMLAAY
                     RWQYIVSGVQHAHFGRLLRSMTTPAQMARIQQALKPIMGAA"
     gene            7914..9440
                     /locus_tag="PKN91_07700"
     CDS             7914..9440
                     /locus_tag="PKN91_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNS27993.1"
                     /translation="MARFDSILGTIGRTPAVRLARLAPPHVELHVKLEAANPMGSVKD
                     RLALGVIEAAERSGALRPGQTVIEATSGNTGIGLAMVCARKGYPLVVVMAENFSVERR
                     RLMRFLGARVVLTPAAAKGSGMLAKAAELAAAHGWFLCRQFENEANADAHSLTTAAEI
                     LEDFATEPLDWFVTGYGTGGTLKGVARVLREHSPHTRIAVCEPDNSQVLGSGITQPRA
                     AQDGTGASHPSFRPHLMQGWAPDFIPKLTEDAPLFIAHGLIDTIVPVSGAEALRLARA
                     LATQEGILAGITGGATLAAALQLAASAPEGTRILAMLPDTGERYLSTPLFADVPADMD
                     AEELAISRSTPGLRFDVTPAAAAAPAPTAAVPAVAAAPAREVPPADAAALGALIDDRH
                     QPVVLFALEWCEFCWSLRRFFDSAGIAFRSVDLDSVEYQQDDRGGRLRAALTARTSMP
                     TIPQLFVGGEFVGGCTDAFDAWKSGRLQQLLARCGRPVTSTAPIDPYGFLPTWLHPRA
                     "
     gene            9461..10879
                     /locus_tag="PKN91_07705"
     CDS             9461..10879
                     /locus_tag="PKN91_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HNS27994.1"
                     /translation="MDSLRHAGRAGGAGDTTASLLQDAARRGVRYLDEIGSRTVAPPP
                     EAIARLAELDTPLPAGPTDPAAVLARLDEIVGPATMGMAGPRFFGFVIGGALPVTVAS
                     NWLATAWDQNAALATATPGVAHVEAVALRWLVDLLGLPPSTEGAFVTGATVANFTALA
                     AARQQQLAKVGWSVEADGLFGAPPISVIVGEEAHPTLFKALGLLGLGRERVVRVPVDK
                     QGRMRAVRLPRIEGPAIVCLQAGNINSGAFDPFTDICPAAHAAGAWVHVDGAFGLWAR
                     ASRQLAPLAVGLELADSWATDAHKWLNVPYDSGLAFVRERGVLPAAMSVSADYLPTGD
                     GARNPADFTPELSRRARGVDIWAALATLGRSGLEEMFERNCRGARRFAAGLAAAGHEV
                     LNDVALNQVVVSFGAPQVNERVVTAVQADGTCWCGRTVWKGRAALRISVCSWATTDAD
                     VEASLAAILRVAAEVIGHRGHG"
     gene            10891..11412
                     /locus_tag="PKN91_07710"
     CDS             10891..11412
                     /locus_tag="PKN91_07710"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS27995.1"
                     /translation="MNDPITIRRATLADAFRLGDIGGATFSETFAHLYEPEDLAAFLQ
                     STHSPESWARRLHDDHLAVWLVEDAGGTAIGYASAGPCKLPVPDLESNAGELFQLYLR
                     ASHHGMKLGSRLLETALAWLEMRGYDPLYVGVWSRNIGAQRLYARFGFRHCGEYEFPV
                     GRQRDHEFILRRA"
     gene            11409..>12353
                     /locus_tag="PKN91_07715"
     CDS             11409..>12353
                     /locus_tag="PKN91_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNS27996.1"
                     /translation="MRGPFVTPFHAGEIARRGTARLRAGLPFIPMHFGQPSAGAPAAA
                     IAAAHRVLDRDPLGYQEIVELRERIAAYYAETHGVQVDLDRILLTAGASAALVAAYAA
                     LFRPGDRVAIMRPGYPAYRNTMCAMRLEPVEVACGPEMDFRPTPALIESLQPVPQGIV
                     IASPANPTGAMLDGAQLAAMLATCRERGIRVISDEIYHGISFGKRAVSALELDPDAIV
                     INSYSKLYRMPGWRLGWMVAPPQLAEQLHVYLINMFLTPSTLAQHAALAAMDEREDLA
                     HWVRVYERNRARLIEGLAAIGITRIVPPDGAFYLYADVG"
BASE COUNT         1908 a         4344 c         4185 g         1916 t
ORIGIN      
        1 tcgacatcta tcagcgcgag ggactgctga cgcgcgcgaa gacgctctcg aagacctggg
       61 aggacgccgt gcacggcctg cgtggccggc cgcacgtgat cgacacgcgc aactacggcc
      121 tgatcgcggg tatcgaattc gaagcgattc ccggccaggc gggcgcgcgg gcgttcgaaa
      181 tcttccgcaa gtgcttcgag cgcggactgc tggtgcgtac caccggcgat atcctcgcgc
      241 tctccccgcc gctcatcatc gagccggcgc acatcgacga aatcttcggc atcctcgccg
      301 acgtgctctg aactggtgcc gggttctcgg tcaaccgaaa acccggcacc atgtcttcac
      361 cagggcagag tggtgctgac cgagaacacc agacggcctt cggtgttgaa gacgtcgtcg
      421 gtgaccttgt cgtcgccgct gaggtcggta cccgtgtact tcagctcgag gccgaagttg
      481 ccgagttcgt agccgaccgc cacggaatag tcgaagtact cgaaatcttc gaacgcatcg
      541 ccgtagttgt agccggcgtg cagcgacagc gagaagttct gcggcagctc gaaggtgtag
      601 ttggcctccg tgtacagcgc atcgttcgtg ccgtcgtact cgaacgcgta ccactgcttc
      661 aggtcgaggc ccttgtagtt gatgccgagc caggcttccg gatacgcctc gatcttcgat
      721 cgcgtcgccg aattgctgga ccccggatag gtgtaccagg tgaggccgac ggtccagccc
      781 gtatcgtcgt tgatcgcgcc gtcgtaaccg gcgtacaggt cgacttcgat gtccccgtcg
      841 acgtcgtcac cgtagtcgat gttgcttccc caggcaccga cgtagaaacc cgtctcgcca
      901 aaggcgtagt cgaggctcgc ctgcagcgcc ggatccttgg cactcagcga cacgccgcgg
      961 aaatcgtagt cgctggtggc ggtgatcgtg gcactgaact gcgcgtgtgc cgtgcccgca
     1021 acagcgagca ggcccagcag cagcgatgtt cgtttgaacc ggttcatgaa tcctcccttg
     1081 ttgaggtgcg accggcatgt actgcaagct gcatgccgga gttttgtgcg ttctgcgtgc
     1141 ctctggcgcg attatacgca ccaatcatgc gcgcggaatg tagattgcgt agatcttggt
     1201 gcagtcttcc agtaccgccc aggtcccgga aaagcccgac ggcacgacga acgcatcgcc
     1261 cttcgcaaag cacatccgcg ttccggaatc gctgaccagt tcgacccgcc cggcggtcag
     1321 cacgcacaat tcgctctccg tgtagcgcac ccgccacacc ccgcgcgtgc tggaccagtg
     1381 gccgacgtga aagcactcgg cgggatcgct ataggcgttc cacgcgtgct gcacgggatc
     1441 gccgctgacc aggcggtcgg ccgcgggccg ggaggcctcg ccctcgccgg cgagtgtcac
     1501 cgggatgatt tccatggcca tcagaagttc gtcctccgcc cgaggtcctc gtacatgcgc
     1561 gcaaggaagg cgcgcacggc cggcgtcgcg tcctccggcc tcatcagctt ctgttcgacc
     1621 agcacgcggg cgatatcggc gaccgagcga tggccgtcga ccagcgacat caggaaggcg
     1681 tggatgcgtg tcgcgagcgc gtgcgagcga agctcggccg gcaacggcac cggttgcgtc
     1741 acatcggcaa gccagacggg cggctcgtgc gcagcgggcg cgggcacctc ggcacgcttg
     1801 cgcgccgacc aggccaccgt cgactcgacg cgggcatgac ggctcgccgg cgagcgcatg
     1861 taggggagcg gctcctcgcg cgtgcgcggc gggtcgaagc ccgcctcctc ggcgagctgc
     1921 agggtctcct cgaggctgaa ctgctgcgcg cgctgcgcgc tggcgaaggc gaccgagccg
     1981 agattcagcc accggccgcc cgtcttcagc agcgcattga cgcgcgccgc gagcgcggga
     2041 aacggcgcct cgacgatatc gatgaaccag ggtgtcacga cgacatcgaa cgcgccgggg
     2101 gcgaagggcg cctgcagcgc atcgcccgcc acgagcacga gccgccgatc ggcggcggcg
     2161 ggcgcgtgca ggcgccgcag caccgcgtga tcggcgatgc tgcgcggggc aatcgggagt
     2221 tcgtagagct cgagccgtcg gccggcatac atctcgcgtg ccacggcgag cagcaatgga
     2281 ttcaggtcca tggcgaccac gagcgcgggc gcaaagctcg agtacaggtc gtaggccagc
     2341 cggcccgccc cgctgccgag caccgcgatg cgcgcgcccg cgagatcgcc gtccgccacc
     2401 gccgcgagca gcgcaagcgt ggcctcgttc tccgcctcgc cccagcacca gtcacgatgg
     2461 atgttggcgt agtagttggt caggccctgc gagccgggca ggcgcgtgcg caaagcctgc
     2521 agcgcctctc gcgcggaggg gtctgcgtcg acccccagcg gctcgagcaa gctcgccagc
     2581 cgctgcgcgt ggtcgtcgag cgcgccagcc agcagctcga ggcgttgccg ggtcagcgcg
     2641 ccgggtgcat ccgcgatccc ggcctggcag gcgcgcgcat cggcgcgcag gtcggcaagc
     2701 agcagggcga gccgcgcccg ccactccgcc gcggcaccct gcggatcacg gaacagccac
     2761 ggcacgccgc ccaggatggg gtaccccgcg ctgcagcggt cgcagatgta ccctgccggg
     2821 ccctcatgga ccgcaccccg ctcgcacctc ggacaccggc taatcccgct cagcacacaa
     2881 cttgcgccat ttctagtacc ctttactcac catgacacgc caacgtggac gcccgaaagc
     2941 caacgggctc gcattacatc atcgcctgta catgatgttg aagcagcaac tcgccgacgg
     3001 caccatcgcc cccggcgcgg cgctgccgag cgaacccgac ctggccctgt cgaccaagct
     3061 ctcgcgtacc acggtgcgcc gcgcgctggc gcgtctcgcc gcagagggcc tgatcgagcg
     3121 ccgccacggc agcggcactt atgccacgat gcagggtgcc gcgcgggcga aggcggtggc
     3181 cagcgccgac tacggccggc tgctcggcga cctgcgcacc atggcacgcg tcaccaaggc
     3241 gcgcctgctg cagtacgcag tgatcgccac gcccccgcgc atcctggccg tcaaccccga
     3301 actcggcacc cgggtgctgt acatgcagcg cacccgcctg taccggggca agcccttcgt
     3361 ggtcatcagc cactatattc ccgagcgcct cgtgaaactg ctgcccaagg cgcagttgaa
     3421 atccgtgccg ccgatcttcc tgttcgagaa gaaaggcatg gtgccgacga tcggcgacca
     3481 ggaggtcacc gcggtggccg ccgaccacgt gttttcgcgt gagctcgccg tcaacatcgg
     3541 ggcgccgctg ctcgcgatcc gccgctcggt gcgcgacagt cgcgggaccc tgctcgagta
     3601 ccacgaagcg ttctacgacc ccgagttgta ttcggtgcag atgcaggtgc gccgcgagcg
     3661 ggtcggcaag agcggcatgc gctggcggcc cggccgctga tctcaccgcc ggcgcgccac
     3721 gagcgcgtcg agttccttcg ccgccgagac cgtgcgcgcg tgttccgcgc cgagctcgcg
     3781 cctgaacacc tcgagcgccg cgcgcagttc ggcttcggcc tcttcgtacc gaccgcgatt
     3841 cgtcagcacc gtggccaggt agcgctgcgc attcgctgcg cgccagtgcc cgtcgcccag
     3901 cgctgcccgc atgtcccgca tgctcgcgcg cagcagcact tcggcctcgg tgtagcgacc
     3961 cagcttgttg agcgcgagcg cgcggccggt acgcgccgcg cccgtatcag gatgatccgc
     4021 gcccagcgtg ctctcgcgga tctcgatcgc gcgcgcgagc gccgcttcgg catcggccca
     4081 tcgcccggtg agcaggtaga ggctaccgag attgttgtgg acagtggcga cctccggatg
     4141 ccgctcgccg aaggccgcca gcaggatcgc gagcgcttcc tggtacacgc tttccgcctc
     4201 gccgacgcgt gatggcatat cggacagcgc attgccgagg ttgttgcggg caatgcccgc
     4261 gtgcggatgt ccccggccat agatgcgctc gatgtcgcga acgaaatcgc gcagcagcgg
     4321 ctccgcccgc tcgatatccc ccctgttgac gagtacctcg gcgaggccgc tgcgcatgtc
     4381 cagcgtcagc gcatcgaggt cgccgccgat ggcgcggccc tgcgcgagcg cgtcggacaa
     4441 caggcgttcc gcctcggcga ggtcgccttg tgcggcacgg atgcgcccga gcaggaagcg
     4501 ggcgcgcacg tagccaagcg tcccgccctg gtctgcggcc tcgaatgcgc gcgcggactg
     4561 cagcgcaagc tgcgcggcct cgtcgagctg gctgcgccgg cgcagcgtat cggccagctc
     4621 gagacgcacc gtttcgcgga cggcgaggtc gccatcgggc accgcagtca cgacggcgag
     4681 cgcctcgcgc gcaagcgact cggacgcaac ccagtcaccc tgctcgcgct tgagcgcggc
     4741 ctggctggcg agcgcctggg cgtaggcttg cggctcccgc tccacagccg actcgcgcag
     4801 caccaccgcc cgcgccagca gcttgcctgc cgcgtcgtag gcgcccaggc cgctgtaggc
     4861 attgcctgcc gcctcgagca gtgcggcgcg cacatccgcg cggccatcgt cgggctgggc
     4921 ctcgaggcgc tgcactccgg catcgagcag ctcgcgggca gtgatctcgc gcgcacctga
     4981 ttgcgcggga ttggcgatct cgaacaggcc gacggtgaac gcgacgatgc ggcgcgcggt
     5041 cgcggcctcg gcattcgccc ggtcacgttc gagcgccgcc tgccgggcga gccaggccat
     5101 gcccgagccg aagccgagcg ccgcgacgaa cagcgaggcg gccgcggtga ccgcgaggcg
     5161 atggcggcgg acgaacttgc cgatgcgata gcgcgcggtg ggcggccccg cgagcaccgt
     5221 atcgttgttg gcatggcgct cgagatcggc ggccagctcc gcgggcgagc tgtagcgcag
     5281 gtgacgatcg ggcgcgagcg ccttcatgac gatccagtcg agatcgccgc gcagacgtgc
     5341 agcccgcgcc ggcgtgaccg ccaccgcact cggtctcacc gcttcgcgat tcgcgatctc
     5401 gccggcgagc gcgaccggat tgtattcgct gccgcggaca tcgtacgggc gcacgccggc
     5461 cagcaactcg aacaggatca cgccgagcga atacacgtcg gtgcgcgcat cgacatccag
     5521 cggtgcgagc tgcgcctgct ccgggctcat gtactcgggc gtgccgagca ggtgcccgac
     5581 gcgggtgtgc gcatccgcgt caccaccgtc ggtcatcgcc gccttggcga tgccgaaatc
     5641 gatgatcctg ggcgtcggcc gcccgtcgat ctcggtaacg aggatgttcg agggcttgag
     5701 gtcgcgatgg atgatgccgc gcaggtgcgc gtgctgcacg cccgcgcaga tctcgccgaa
     5761 caactcgagc cgcgattcca cggcgaggcc gtggtcctcg cagtagcggg tgacggccgt
     5821 accggccaca tactccatcg caaagtaagg gcgcccgtcg ccggtcgtac cggaatcgta
     5881 gatgcgtgcg atgttcgggt gcgtgagcat cgcgagcgcc tggcgctcga gttcgaagcg
     5941 cgccaacact tcgcgcgtgg cgagaccgaa cttcaggatc ttgagcgcga cgttgcgccg
     6001 catcggcgcc cgctgctcgg cgaggaacac ctcgcccatc gccccctcgc cgatgcgctc
     6061 gatgacacga tagggtccga tctggagcgg tgctgcaagc cggggtatct ccggcggcgc
     6121 gccgccgagg aacgaatcgg gcgcctcatc gtgcgcgctc agcagacggc ggacacgctc
     6181 acgcagcgcc gggtcgggac agtccgccag caaccgccgg cgcgtcgcct cgtcggcctc
     6241 gacgcaggcg tcgaacagtt cctgttcagc atcatcgacg ctcatgcgcc cgccgacagc
     6301 cgctcctgca gccaggcgcg ggcgtagacc cagtcgcgct tggccgtggc gaccgaaatc
     6361 ccgagcgcct cggctgcctc ctcgatgctg aggcccgcga agaagcgcag cgtgacgagt
     6421 tcggcaaggc gcggacgccc ggcggcgagc tccgtcagcg ccgcatcgac cagcaggaca
     6481 tcggcgtcgt cctcgggaat cgggatttcg agccccgtca acgtgacccg cacggcatcg
     6541 ctgccacgct tctgcgcatg gcgtcgccgc gcctgctcga cgagcacgcg gcgcatcgcc
     6601 tcggctgcgg cgccgaagaa gtgacggcgg ttctgccaga cggcgccgtt cggcgcgagg
     6661 cgcagaaaag cctcgttgat gagcgcggtc ggctgcaggg tgtccgcgcg cccttcccgg
     6721 cgcatgttgg cgcgcgccat gcggtgcagt tcgtcgtaga ccaccctgta caggcgttcg
     6781 aacgcctgcc ggtcccccga gccggccgcc tccagcagca aagttacgtc cgtgtgagcc
     6841 acctgacgcc aattctacgc atgagggggt gaacgggtca cttcgacccg gaccagggag
     6901 ggtcgatgaa cgctgtcgtt gccgaaagag tcccggatgc aggtcgggcc gcgcgctatg
     6961 cgcgctgcgt caatatgtcc aagaaggtgg agtgggagat cgagcgcgat ctgctgcgcg
     7021 ggcgcgattt cgacttctcg cgcaagttcc tgccggatgg actgtcgctg gtggaacgcc
     7081 tgtcgctggc gccaggcgag gcacgcctga tgagccaggt gcagggccgc acctacgcct
     7141 acattttcgg actcgtggag cgcttcatca gcgcagggat ggtcgacgcc gcgggcaagc
     7201 atgccttcgg caaccaggtg gccatggaag gcctgctgcg cttttccggc gaggagctga
     7261 agcaccagga actgttccgg cgcatggaac agatgatggc gcaggccatg ccggccggct
     7321 attcacaggt cgcggacccg aacgatgtcg cccgcgccgt gctcgagcgg cgacggtggg
     7381 cggtgctcgc cctcacctgc cacatcgaac tgttcgtgca ggcgcactac gagcagagca
     7441 tcgccgccga ggcggacatc tgcccgctgt ttcgcgatgt gttcctgtac cactggaagg
     7501 acgaatgcca gcacgtcatg ctggacgaac tcgagtggac ggccgagcac gcgcgactca
     7561 cgccggccga acacgatgcg gcggtcgacg acctgatcgc gctggtcggc gcggtcgacg
     7621 ggctcctgca gggccagtcg cgcagcgatg cagcctattt catgtctgct gcgggacgtg
     7681 aattcccggc tgccgaggcc gcgcagatcg aggccgccat gctcgccgcc tatcgctggc
     7741 agtacatcgt ctcgggtgtg cagcacgcgc atttcggccg gctgctgcgc agcatgacga
     7801 cgccggcgca gatggcccgc atccagcagg cactgaagcc gatcatgggg gcggcatgag
     7861 agcgcggctg cgcgccgtcg agccggcgcc atggtgcgag gccaggcgct gacatggcac
     7921 ggttcgacag cattctcggg accatcgggc gtacgccagc cgtgcggctc gcacggctgg
     7981 cgccgccgca cgtggaactc cacgttaagc tcgaggccgc aaatccgatg ggctcggtca
     8041 aggaccgtct cgccctcggc gtgatcgaag ctgcggagcg cagcggcgcc ttgcggccag
     8101 gccagacagt catcgaggcg accagcggca ataccggcat cggtctcgcg atggtctgcg
     8161 cgcgcaaggg ctacccgctc gttgtcgtga tggccgagaa cttcagcgtc gagcggcgcc
     8221 ggctgatgcg cttcctcggt gcgcgggtcg tgctgacacc ggccgccgcc aagggcagcg
     8281 gcatgctggc caaggcggcg gagctcgccg ccgcccatgg ctggttcctg tgccgccagt
     8341 tcgagaacga ggccaacgcg gacgcgcatt cgctgaccac ggccgccgaa atcctcgagg
     8401 atttcgcgac cgagccgctc gactggtttg tcaccggtta cggcaccggg ggcacgctga
     8461 agggcgtcgc acgtgtgctg cgcgagcact cgccgcacac gcgcatcgcc gtgtgcgagc
     8521 cggacaactc acaggtgctc ggcagcggca ttacccagcc gcgcgccgcg caggacggta
     8581 ccggggcgag tcatccgagt ttccgtccgc acctgatgca gggttgggcc cctgatttca
     8641 tccccaagct caccgaagat gcgccgctct tcatcgcgca cgggctgatc gacacgatcg
     8701 tgccggtgag cggtgccgaa gcgctgcggc tcgcccgcgc gctcgcgacc caggaaggca
     8761 tcctcgccgg catcaccggg ggcgcgacgc tcgccgcggc gctgcaactc gcggcgagtg
     8821 cgcctgaggg gacgcgtatc ctcgcgatgc tgcccgacac cggcgagcgc tatctcagca
     8881 cgccgctgtt cgccgacgtg ccggccgaca tggatgccga ggaactcgcg atctcgcgtt
     8941 cgacgcccgg cctgcgcttc gatgtcaccc ccgccgccgc ggcggcccca gcccccaccg
     9001 ccgcggtccc cgccgtggcc gccgcacccg cgcgcgaggt gccaccggcc gatgccgcgg
     9061 ccctcggcgc actgatcgat gatcgccacc aacccgtcgt gctgttcgca ctcgaatggt
     9121 gcgagttctg ctggtcgctg cggcgattct tcgacagcgc cggcatcgcg tttcgctcgg
     9181 tcgacctcga ttcggtcgag taccagcagg acgatcgcgg cgggcgactg cgcgccgcgc
     9241 tcactgcgcg cacctcaatg ccgacgattc cgcaactatt cgtcggcggc gaattcgtcg
     9301 gcggctgcac cgacgccttc gacgcgtgga aatcggggcg actgcagcag ctgctggcac
     9361 gctgcgggcg gccggtcacc agcaccgcgc cgatcgatcc ctacgggttc ctgccgacct
     9421 ggctgcatcc gcgggcatga tgcggtacaa gggcagactc atggacagct tgcgtcatgc
     9481 gggacgagcc ggcggcgccg gcgacacgac cgcgtcgttg ttgcaggatg ccgcgcggcg
     9541 cggggtgcgc tatctcgacg agatcggttc gcggaccgtc gctccgccac ccgaggcgat
     9601 agcccgcctc gccgagctcg acaccccgct gcctgccggg ccgaccgacc cggcagccgt
     9661 gctggcgcgg ctcgacgaga tcgtaggacc cgccaccatg ggcatggccg gtccgcgttt
     9721 cttcggtttc gtgatcggcg gcgcactacc ggtcaccgtg gcgtcgaact ggcttgcgac
     9781 tgcgtgggac cagaacgccg cgctcgcgac cgcaacgccc ggcgtggcgc acgttgaagc
     9841 cgtcgcactg cgctggctgg tcgacctgct cggcttgcca ccctccaccg agggcgcgtt
     9901 cgtgacaggc gcgacagtcg ccaacttcac cgcgctcgcg gccgcgcgcc agcagcagct
     9961 ggcgaaggtc ggctggtcgg tcgaggccga cggactgttc ggcgccccgc caatcagcgt
    10021 gatcgttggc gaggaggcgc atcccaccct gttcaaggca ctcggcctgc tcggtctcgg
    10081 gcgcgagcgt gtcgtgcgag taccggtcga caaacagggc cgcatgcggg cggtccgtct
    10141 gccgcgcatc gagggtccgg cgatcgtttg cctgcaggcc ggcaacatca acagcggcgc
    10201 gttcgatccg ttcaccgata tctgtcctgc cgcgcacgcc gccggcgcgt gggtccatgt
    10261 cgatggcgca ttcggcctgt gggcgcgggc atcgaggcaa ctcgcaccgc tcgctgtcgg
    10321 cctcgaactc gccgattcct gggccaccga tgcgcacaag tggctcaatg tgccctacga
    10381 cagcggcctc gccttcgtgc gcgagcgcgg cgtgctgccc gccgccatgt cggtcagcgc
    10441 cgactacctg cccactggcg atggcgcgcg caaccccgcc gatttcacgc ccgaactgtc
    10501 gcgccgtgcg cgcggcgtcg acatctgggc cgcgctcgcg acgctcggcc ggagcgggct
    10561 tgaggaaatg ttcgagcgca actgccgcgg cgcgcgccgc tttgccgcgg gcctcgcggc
    10621 ggcaggccac gaggtgctga acgatgtcgc gctgaaccag gtggtcgtgt cattcggcgc
    10681 gccgcaggtc aacgaacggg tcgtgacggc cgtgcaggcc gacggcacct gctggtgcgg
    10741 ccgcacggtg tggaaaggtc gcgcggcgct gcgcatcagc gtgtgttcct gggcaacgac
    10801 cgatgccgac gtggaagcga gcctcgcggc catactgagg gtggcggccg aggtcatcgg
    10861 ccatcgtgga cacgggtaga atcgccatgc atgaacgacc cgatcacgat tcggcgtgcg
    10921 accctcgccg atgcgttccg gctgggtgac atcggcggcg cgacgttctc cgagactttc
    10981 gctcatctct acgagcccga agatctcgcc gcgttcctgc agtcgacgca ctcgcccgaa
    11041 agctgggcgc ggcgcctgca cgatgatcac ctcgccgtgt ggctggtcga ggacgccggc
    11101 ggcacggcca tcggctatgc gagcgccggg ccctgcaagc tgcccgtgcc cgatctcgaa
    11161 tcgaatgccg gcgagctgtt ccagctctac ctgcgcgcca gtcatcacgg catgaagctc
    11221 ggctcgcgcc tgctcgagac ggcgctcgcc tggctcgaaa tgcgcggtta cgacccgctt
    11281 tacgtcggag tctggagccg caatatcggc gcgcagcgac tgtatgcccg tttcggcttc
    11341 cggcactgcg gcgaatacga gtttcccgtc gggcgtcagc gcgaccacga gttcatcctg
    11401 aggcgcgctt gagagggccg ttcgtcacgc cgttccacgc cggcgaaatc gcacgtcgcg
    11461 gcactgcacg cctgcgggcg gggctgccgt tcatcccgat gcatttcggc cagccgtccg
    11521 ccggtgcgcc ggccgcggcg atcgcggccg cccatcgcgt gctcgatcgc gatccgctcg
    11581 gctatcagga aatcgtcgaa ctgcgcgaaa ggatcgccgc gtactacgcc gagacgcacg
    11641 gcgtacaggt cgacctcgat cgcatcctgc tgaccgcggg cgcgagcgca gcgctggtag
    11701 ccgcctacgc ggcattgttc cgccccggcg atcgggtcgc gatcatgcgc cccggttacc
    11761 cggcctaccg caacacgatg tgcgcgatgc gcctcgaacc ggtcgaggtc gcctgcggcc
    11821 cggagatgga cttcaggccc acgcccgcgc tgatcgagtc gctgcagccg gtgccgcagg
    11881 gcatcgtcat cgccagtccc gccaatccga ccggcgccat gctcgacggc gcgcagcttg
    11941 ccgcaatgct cgcgacctgc cgcgaacgcg gcatccgcgt gatctcggat gaaatctacc
    12001 acggcatcag cttcggcaag cgcgcggtca gtgcgctcga actcgatccg gacgcaatcg
    12061 tcatcaacag ctattccaag ctctaccgga tgccgggctg gcgactcggg tggatggtcg
    12121 ccccgccgca gctggccgaa cagctgcacg tctacctgat caacatgttc ctgacgccct
    12181 cgacgctggc gcagcacgcg gcgctcgcgg ccatggacga gcgcgaagac ctcgcgcact
    12241 gggtgcgcgt ttacgagcgc aaccgcgcac gcctgatcga gggcctcgca gcgatcggca
    12301 tcacgcgcat cgtgccgccc gacggcgcgt tctacctgta tgccgacgtg ggg
//
LOCUS       DAOQVY010000069        12117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_5933_length_12117_cov_5.297049, whole genome shotgun sequence.
ACCESSION   DAOQVY010000069 DAOQVY010000000
VERSION     DAOQVY010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12117
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5933_length_12117_cov_5.297049"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PKN91_07720"
     CDS             <1..486
                     /locus_tag="PKN91_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNS27997.1"
                     /translation="LAQPRVFVHRDYHSRNLMIVDEDNPGIIDFQDALEGPIAYDLVS
                     LLKDCYIAWPRARVLGWLREYRRRIAREGLPAGTSEREFVRWFDLIGVQRHLKVLGIF
                     ARLWYRDGKRGYLSDLVLTLDYVRDACARYAELQNFARWLEARVVPGLAAANARELSL
                     R"
     gene            483..1157
                     /locus_tag="PKN91_07725"
     CDS             483..1157
                     /locus_tag="PKN91_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNS27998.1"
                     /translation="MRAMILAAGRGERMRPLTDTVPKPLLTVRGKPLIAYHLEALARA
                     GVREVVVNLAWRGGQIRAVLGDGREFGLHIVYSDEGEVAYETGGGIFNALPLLGADPF
                     LVVNGDVWTRFDFAGLRIGAGADAHLVLVPNPPQHPRGDFTLQDGVVGEDASPDARRY
                     TYSGIGIYRPEFFAGCTPGRFPLLPLLRRSIAAGRLHGELFEGEWQDIGTPARLAALE
                     GANPLK"
     gene            1162..2199
                     /gene="lipA"
                     /locus_tag="PKN91_07730"
     CDS             1162..2199
                     /gene="lipA"
                     /locus_tag="PKN91_07730"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNS27999.1"
                     /translation="MSELKDIPVVREVRPAARSGEKYQTPQGFTAIKDGIKPRGEADR
                     RGLLGKPRWLKAPLAAGAGYDSVRRAVRDHRLATVCEEAKCPNIGECWNAGTATIMLM
                     GAVCTRACRFCSVDTGNPRGWLDHDEPQHAARTVQLMKLRYVVLTSVDRDDLPDGGAA
                     HYAACIREIKALNPETAVEALTPDFQGVLRDVETVVDSGLEVFAQNVETVRRLTHPVR
                     DPRAGYDRTLAVLAHAKRHRPAVVTKTSLMLGLGETDEEIAATMDDLRAIDVDILTLG
                     QYLRPTMNHLPVERYVSPQEFDRYREWGLAKGFRECVSGPLVRSSYRAEQALNGNNVG
                     LDNHAAAARNA"
     gene            2174..2839
                     /gene="lipB"
                     /locus_tag="PKN91_07735"
     CDS             2174..2839
                     /gene="lipB"
                     /locus_tag="PKN91_07735"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNS28000.1"
                     /translation="MLPLPATPEFTGAADGAQPAVKWLGRVDYEPTWRAMQRFTDERG
                     ADTPDEIWLLEHPPVFTLGMNADPAHLLAPGDIPVVQIDRGGQVTYHGPGQLVVYPLI
                     DLRRAGLGIRELVTALEKSVIDYVAMLGIYAETKPRAPGVYVHGRKLASIGIRVRRHA
                     SYHGLALNVSLDLAPFKRINPCGYQGLEMCRLVDLGGPGDVREVGDAYVPWLLARLAS
                     VSR"
     gene            complement(2849..3085)
                     /locus_tag="PKN91_07740"
     CDS             complement(2849..3085)
                     /locus_tag="PKN91_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28001.1"
                     /translation="MRGTSSRTFLPIAGALAVALLLSACGLAETAGTAAATGASAAEQ
                     ARQAREIEAEVEQQVLDAQAEAKRLRDEAEAAAN"
     gene            complement(3101..3364)
                     /locus_tag="PKN91_07745"
     CDS             complement(3101..3364)
                     /locus_tag="PKN91_07745"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HNS28002.1"
                     /translation="MSDLTLLEFPTDYPIKVVGRAGELLRTEVDAIMRRHVADLDSQR
                     TSERLSSGGNYLSISYTIVARSEAQVVALAAELAAQPHVIMVI"
     gene            complement(3361..4233)
                     /locus_tag="PKN91_07750"
     CDS             complement(3361..4233)
                     /locus_tag="PKN91_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HNS28003.1"
                     /translation="MAEPLPTAYLNGSFLPLREARISPLDRGFLFADGVYEVIPVFGG
                     RPFRFGPHFARLARSLAEIRMREPHDLEGWRTICRELIAANGGGDQYLYVQVTRGAEY
                     GRNHAPLPDLEPTVFAFAAPLPRADARQLATGAACITAADTRWARCDIKSIALLPNIL
                     LRQLAVDAGAGETILLRDGWLMEGSASSVHVVVDGEVLSPPNSTWILPGTTRSVVEEL
                     LLELGIPHRSTPVAEATLRAADEIWLGAATRDITAVTRLDGQPVGTGAPGPVWQRVHR
                     GFRQLVTRVAGAPW"
     gene            complement(4246..5385)
                     /locus_tag="PKN91_07755"
     CDS             complement(4246..5385)
                     /locus_tag="PKN91_07755"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNS28004.1"
                     /translation="MRHSRRLAVLCLVACAFASAHAALPIPKPPAIDARALILIDHDS
                     GRVLAESNADERMEPASLTKVMTGYIAFEALKDGRLTLTEEIPISERAWKSEGSRSFV
                     QVGTRIPVEVLLKGMIVQSGNDATIALAERLGGSEEGFAQIMNAYAKKLGMRNTNYEN
                     STGLPGANHYTTARDMATLARAVIRDYPEYYQWYSLREFVWNNIRQQNRNGLLARDAS
                     VDGIKTGHTESAGYCLATSAKRDGMRLVSAVFGTKSMRAREDASAALLNYGFTFYETV
                     KLKGRGETVLAPRLYKGEEENIAIGPRADVYVTVGRGDAAQVTTAATANEPLLAPADP
                     ATAAGELVVSVGNDVVSRVPLYPLKAVPEGGWWTRMIDGLLLMWH"
     gene            complement(5434..6279)
                     /locus_tag="PKN91_07760"
     CDS             complement(5434..6279)
                     /locus_tag="PKN91_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNS28005.1"
                     /translation="MRRIVLPAAALALLPLVLAGCGSRDTKSAPARAPVPAATPAPPA
                     SVAAIPDAVPRAEPRSLRGNPPFYEVFGQRYVVLPSAEGYIERGVASWYGPGFHAALT
                     SMGEPYDMYAMTAAHKTLPIPCYARVTNLANGRSVVVRINDRGPFVAGRIIDLSYTAA
                     AKLDMLQKGTAFVEVQVITPGTAVAVAPAPLATPAATSLAPTAGEGLYIQAGAFADGG
                     NARRLVERLREGGIGNAFVRSGEVGGRTLHRVRIGPVGSVAEYDALAARLAKFGITDA
                     RLALD"
     gene            complement(6276..7289)
                     /gene="mltB"
                     /locus_tag="PKN91_07765"
     CDS             complement(6276..7289)
                     /gene="mltB"
                     /locus_tag="PKN91_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HNS28006.1"
                     /translation="MRTLATALALACLALPAAHADGVFAGTDRFDLARADIRQYVESA
                     AASEGIAPEDFYGLLAKAEPKPKIIEAMERPAEKVSPWWEYRKRFLTPQRVREGVAFW
                     QQHRELLERISAERGVAPEYIVSILGVETYYGRITGNYRVLDALATLAFDYPPRSPFF
                     SKELTQFLLLSREESVDPTTALGSYAGAMGAPQFMPSSYRRYAVDGDGDGRRDLFASW
                     DDVVASIANYFREHDWQVGGPVLIEARLAEGRRLELDPRNLSLTDTLDSARAKGLVFE
                     STLPGDSKVILIPAETEHGTGVRIGFANFVTITRYNRSVRYAMAVHDLAQAVAAEVFE
                     RGE"
     gene            complement(7286..8422)
                     /gene="rodA"
                     /locus_tag="PKN91_07770"
     CDS             complement(7286..8422)
                     /gene="rodA"
                     /locus_tag="PKN91_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNS28007.1"
                     /translation="MTFGSIDSSYTRRTLTRSARALASLKLDGPLLIGLALIALYGLI
                     VMYSASGQNVGAVIKSATRLGIGIVVMLLVAQVKPGFLRRATPIIYVAGVVLLIVVDV
                     VGYVGKGAQRWLDLGLIRFQPSEFMKIAVPMMCAWYLHERPLPPSTPHLLVTVMIIFV
                     PAALTIAQPDLGTGLLITIAGVLVILMAGLQLRVILALGGLAAIGAWFGWSFLHEYQQ
                     KRVLMFLDPQSDPLGAGYHIIQSQIAIGSGGIFGKGWMNGSQGQLEFLPERSTDFIFA
                     VIGEEFGLVGLALLLFLYLFVVGRAMFLAMQTQETFARLLSGSIAITFFVYVFINTGM
                     VSGLLPVVGVPLPLVSYGGTSMVTLMAGFGILMSLYSHRKLIRS"
     gene            complement(8419..10302)
                     /gene="mrdA"
                     /locus_tag="PKN91_07775"
     CDS             complement(8419..10302)
                     /gene="mrdA"
                     /locus_tag="PKN91_07775"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNS28008.1"
                     /translation="MPANVRIKDHWKEQRLFERRAMAAGAIIGVLALVLFARLVTLQV
                     VRHEYYTDLSQGNRVRIEPLPAPRGLILDRNGTVLAENRPAYQLELVREEVPDLDATL
                     RRLSEIGLIAADDIDDTRKLVRSHRSFDSVPIRLRLDDEQIATFAVHRFEFQGVDIRT
                     RLARSYPYGELAVHALGYVGAISEQDLGRIDRAAYSGTTLIGKLGAESAYESELHGQN
                     GSREILVNAQGRSVQRQGALVPNLITVQPVAGKDLVLAMDIEVQRVAEAELGARRGAV
                     IAIDPANGDVIALVSRPGYDPNAFGRGISRVEYQALLDDIDRPLFNRALRGTYPSGST
                     IKPAIALAGLVHGEVIPTETRFCNGVFRLPGSSRMFREGRSGRHGAVALVDSIARSCD
                     VYFYDLASRLGVDRISGFLAPFGFGSLTGIDIGGEKPGLLPSREWKRKAFSDPADQVW
                     FPGETVNFGIGQGYFLVTPLQLAHYTSILANHGTSYKPRLVTALRDPVSGAVRQLAPQ
                     KVAAISATPEQWDIVTQGMIGAMRGRGTAAATAGRNMTYTIAGKTGTAQVFSVGQTEK
                     YDEKTVAERLRDHSWFIAFAPAEAPRIAVAVLVENGGFGSTGAAPIARKVMDAWLLKG
                     GGA"
     gene            complement(10309..10788)
                     /gene="mreD"
                     /locus_tag="PKN91_07780"
     CDS             complement(10309..10788)
                     /gene="mreD"
                     /locus_tag="PKN91_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNS28009.1"
                     /translation="MNVRVREPRGPLLLSALIALIFQVVPLPEWLAIARPAFLVLVVL
                     YWSIVAPRAGGLLFPFVCGLALDVFRGAVLGEHALALTIVAFLMMRFHLLIRAKPLFE
                     QALIVFAALMLYEFVVWCIDGWTGHTLNSPLRWLHPVTGGLVWPVVFGVLSRTHRPR"
     gene            complement(10785..11684)
                     /gene="mreC"
                     /locus_tag="PKN91_07785"
     CDS             complement(10785..11684)
                     /gene="mreC"
                     /locus_tag="PKN91_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNS28010.1"
                     /translation="MTIFGSGRAVHSRVAATGPRFTFYVALAIVLMFLDQRGNWLEYA
                     RYALSAAAYPVQLAVNSPSAAWHWVRDNFVSRERLAAENAALREANKALELRALRIES
                     LEAENAQLRELEAALPDLAERWLVAEVLAVELSTLRQRVVVNKGAADGVFKGQSVLAR
                     GGLMGQVMRVGPWSAEVILISDPEHAVPVQVARSGLRTIAVGQGNTGVMTLPYLPVNS
                     DIKVDDLLVTSGLGGVFPAGYPVARVLDVGRDPGQPLAQVHAQPLAAIDRDREVMLVW
                     FRAGHPAAPAVESTVLPSPEAAP"
     gene            complement(11713..>12117)
                     /locus_tag="PKN91_07790"
     CDS             complement(11713..>12117)
                     /locus_tag="PKN91_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019408857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNS28011.1"
                     /translation="TAERIKIEIGSAYPGQQVRELSVKGRNLSEGVPRAFTLNSNEIL
                     EALQEPLQGIVSAVKQALEQTPPELGADVAERGIVLTGGGALLRDIDKLLMEETGLPV
                     LIADDPLTCVARGGGRILELMDEMGPGHFGLE"
BASE COUNT         1939 a         4054 c         4265 g         1859 t
ORIGIN      
        1 ctcgcgcagc cgcgcgtgtt cgtgcatcgc gactaccact cgcgcaacct gatgatcgtg
       61 gacgaggaca atcccggcat catcgacttc caggatgcgc tcgaggggcc gatcgcgtac
      121 gacctcgtgt cgctgctgaa ggactgctac atcgcctggc cgcgcgcgcg cgtgctcggc
      181 tggctgcgcg agtatcgccg ccgcattgcg cgcgagggcc tgccagcggg gacgagcgag
      241 cgcgagttcg tgcgctggtt cgatctcatc ggcgtgcagc ggcacctgaa ggtgctcggc
      301 attttcgcgc gcctctggta tcgcgacggc aagcgcggct acctgtccga cctcgtgctg
      361 actctcgatt acgtgcgcga tgcctgcgcg cgctacgcgg agctgcagaa tttcgcgcgc
      421 tggctcgagg cccgcgtggt tcccggactc gccgccgcga acgcgcggga actctcgctg
      481 cgatgagggc catgatcctg gcggccgggc gcggcgagcg catgcggccg ttgaccgaca
      541 ccgtaccgaa gccgctgttg acggtccgtg gcaagccgct gatcgcctac cacctcgagg
      601 cgctcgcgcg cgcaggagtg cgtgaggtcg tggtcaacct cgcgtggcgt ggcgggcaga
      661 ttcgcgcagt gctcggcgac ggacgcgagt tcggcctcca catcgtctac agcgacgagg
      721 gcgaggtcgc gtacgaaacg ggcggcggca tcttcaatgc gctgccgctg ctcggtgcgg
      781 acccgttcct ggtcgtcaat ggcgacgtgt ggacccgctt cgatttcgcg ggcctgcgca
      841 tcggggcggg cgccgatgcg cacctcgtgc tggtgccgaa tccgccgcag cacccgcgcg
      901 gcgacttcac gctgcaggat ggggtggtgg gcgaggacgc ctcaccggac gcacggcgtt
      961 acacctactc cggcatcggc atctaccggc cggagttctt tgcgggctgc acgcccggcc
     1021 ggttcccgct gctgccgctg ctgcgacgct cgatcgctgc cgggcgcctg cacggcgagc
     1081 tcttcgaggg cgagtggcag gatatcggca cgcccgcacg cctcgccgcg ctcgaggggg
     1141 cgaatccgct aaaatagcta aatgtccgag ctcaaagaca tccccgtcgt gcgcgaggtc
     1201 cggcccgccg cccgcagcgg ggaaaaatac cagaccccgc aaggctttac ggccatcaag
     1261 gacggcatca agccgcgcgg cgaggccgac cgccgcgggc tgctcggcaa gccgcgctgg
     1321 ctcaaggcgc cgctcgccgc gggcgcgggc tacgacagcg tcaggcgcgc cgtgcgcgac
     1381 cacaggctcg caaccgtctg cgaagaggcc aagtgcccga acatcggcga gtgctggaac
     1441 gcgggtacgg ccacgatcat gctgatgggc gcggtgtgca cgcgggcctg ccgcttctgc
     1501 tcggtcgata ccggcaatcc gcgaggctgg ctcgaccatg atgagccaca gcatgccgcg
     1561 cgcaccgtgc agctgatgaa gctgcgctac gtcgtgctca cgtcggtcga tcgtgatgac
     1621 ctgcccgatg gcggcgccgc gcactatgcg gcgtgcatcc gcgagatcaa ggcgctgaat
     1681 cccgagaccg cggtcgaggc actgacgcct gatttccagg gcgtgctgcg cgacgtcgag
     1741 acggtggtgg attccggcct cgaagtattc gcgcagaacg tcgagacggt gcggcgcctc
     1801 acccacccgg tgcgcgatcc gcgtgcgggc tacgaccgca cgctcgcggt gctcgcgcac
     1861 gccaaacgcc atcggcccgc ggtcgtcacc aagaccagcc tgatgctggg cctcggcgag
     1921 accgacgagg aaatcgccgc gaccatggat gacctgcgcg cgatcgacgt cgacattctg
     1981 acactcggcc agtacctgcg tccgacgatg aatcacctgc cggtcgagcg ctacgtctcg
     2041 ccgcaggagt tcgaccgcta tcgcgaatgg ggcctggcga agggcttccg cgaatgcgtg
     2101 tcggggccgc tcgtgcgttc gagttatcgc gccgaacagg ccctcaacgg caacaacgtc
     2161 ggcctcgaca accatgctgc cgctgcccgc aacgcctgag ttcacgggcg ccgccgacgg
     2221 cgcgcagcct gccgtcaagt ggctcggccg ggtcgactac gagcccacgt ggcgtgcgat
     2281 gcaacgcttc accgacgagc gcggcgccga cactcccgac gagatctggc tgctcgaaca
     2341 tccgccggtg ttcacgctcg gcatgaatgc cgatcccgcg cacctgctcg cgccgggcga
     2401 cattccggtc gtgcagatcg accgcggcgg gcaggtcacc taccacggcc ccgggcaact
     2461 ggtcgtgtat cccctgatcg atctgcggcg cgcgggtctc ggtattcgcg agctcgtcac
     2521 ggcactcgag aagtcggtga tcgactatgt cgccatgctc ggcatctacg cggaaacgaa
     2581 gccgcgcgcg ccgggtgtct acgtgcacgg ccgcaaactg gcgagcatcg gcatccgcgt
     2641 gcgtcgccac gcgagctacc atggcctcgc gctcaacgtg agcctcgatc tcgcgccatt
     2701 caagcgcatc aatccgtgcg gctaccaggg actcgaaatg tgccgcctcg tcgatctcgg
     2761 cgggcccggg gacgtgcgcg aagttggcga tgcctacgtg ccgtggctgc tggcgcgcct
     2821 tgcgtcggtg tcgcgctagg ccagcagctc agttcgcggc ggcttccgcc tcatcccgca
     2881 gccgcttcgc ctcggcttgc gcgtccagca cctgttgctc gacctcggcc tcgatttccc
     2941 tggcctggcg ggcctgctcc gctgccgagg ctccggtggc cgcagcggta ccggcggttt
     3001 ccgcgagccc gcatgccgac agcagcagcg cgacggcgag cgcgccggcg atcggcagga
     3061 atgtgcggct tgatgtgcct ctcatgtgcg actccctggc tcagatgacc atgatcacgt
     3121 gcggctgcgc cgcgagttcg gcggccagcg ccacgacctg cgcctcgctg cgcgcgacga
     3181 tcgtgtagga aatggacaga tagttgccgc ccgacgacag ccgttcgctg gtccgttgcg
     3241 agtcgagatc ggcgacatgg cggcgcatga tcgcatcgac ctcggtgcgc agcagttcgc
     3301 cggcgcgacc gaccaccttg atagggtagt cggtcgggaa ctcgagcagc gtgaggtcgc
     3361 tcaccagggc gctccggcaa cgcgcgtcac gagctgcctg aagccgcgat ggacgcgctg
     3421 ccacacgggc ccgggcgcac cggtgccgac cggttggccg tcgaggcgcg tgacggcggt
     3481 gatgtcgcgt gtggccgcgc cgagccagat ctcgtcggcg gcgcgcagcg tggcttccgc
     3541 aaccggtgtg ctgcggtgtg ggatgccgag ctcgagcaac agttcctcga cgacgctgcg
     3601 ggtcgtgccc ggcaggatcc acgtgctgtt gggtggagag agtacttcac cgtcgacgac
     3661 gacgtgcacg ctcgacgcac tgccttccat cagccagccg tcgcgcaaca ggatggtctc
     3721 gccggcgccc gcgtcgaccg cgagctggcg cagcaggatg ttgggcaaca gcgcgatcga
     3781 cttgatgtcg cagcgcgccc agcgcgtgtc ggcggcggtg atgcacgcgg cgcccgtggc
     3841 gagctgtcta gcgtccgcgc gtggcagcgg cgcggcgaac gcgaacacgg tcggctcgag
     3901 gtcgggcagc ggcgcatggt tgcggccgta ttccgcgccg cgtgtgacct ggacatacag
     3961 gtactggtcg ccgccgccgt tcgcagcgat cagctcgcgg cagatcgtgc gccaaccctc
     4021 gaggtcgtgc ggctcgcgca tgcggatctc ggcgaggctg cgcgcgagcc gcgcgaaatg
     4081 cggcccgaag cggaacggcc gcccgccgaa caccgggatg acttcataga ccccgtcggc
     4141 gaacaggaag ccccgatcga gtggcgagat ccgtgcctcg cgcagcggca ggaagctgcc
     4201 attcaggtac gcggtcggca gcggttcggc catcggcagc gctcctcagt gccacatcaa
     4261 caggaggccg tcgatcatgc gggtccacca gccaccctcg ggcacggcct tgagcggata
     4321 gagcggtacg cgcgacacga cgtcgttgcc gaccgagacg acgagttcgc cggcggcggt
     4381 ggcgggatcg gccggcgcga gcagcggctc gttggcggtg gccgccgttg tcacctgcgc
     4441 ggcatcgccg cgaccgacgg tcacgtacac atcggcacgc ggcccgatcg cgatgttctc
     4501 ctcctcgccc ttgtacaggc gcggcgcgag cacggtctcg ccgcggccct tcagcttgac
     4561 ggtctcgtag aacgtgaagc cgtagttgag cagtgcggcg ctcgcatcct cgcgcgcacg
     4621 catcgatttc gtgccgaaca ccgccgagac gagccgcatg ccgtcgcgct tggccgaggt
     4681 ggcgaggcag taaccggccg attcggtgtg cccggtcttg atgccgtcga cgctcgcatc
     4741 ccgcgcgagc agcccgttgc ggttctgctg cctgatgttg ttccagacga actcgcgcag
     4801 cgagtaccac tggtagtact cgggataatc gcgaatgaca gcgcgggcga gcgtcgccat
     4861 gtcgcgcgcg gtcgtgtagt ggttggcgcc cggcagaccg gtgctgttct cgtaattcgt
     4921 gttcctcatg ccgagcttct tcgcataggc attcatgatc tgcgcgaagc cttcctcgct
     4981 gccgccgagg cgctcggcga gcgcgatggt cgcatcgttc cccgattgca cgatcatgcc
     5041 cttcagcagc acctcgaccg ggatgcgcgt gccgacctgc acgaacgagc gcgacccttc
     5101 gctcttccac gcacgctcgc tgatcgggat ttcctcggtg agggtcaggc ggccatcctt
     5161 cagggcctcg aaggcgatgt agccggtcat caccttggtg aggctcgcgg gctccatgcg
     5221 ctcgtcggca ttggattcgg cgagcacgcg gccgctgtcg tggtcgatga ggatcagcgc
     5281 gcgggcatcg atcgccggcg gcttcgggat cggcagggcg gcatgcgccg aggcgaatgc
     5341 acaggcaacg agacagagta cggcaagtcg tcggctatgg cgcatccgac atctccagcg
     5401 gctgaaaggg aatgacagcg caggattgta gcgtcagtcg agcgcgaggc gtgcgtccgt
     5461 gatgccgaac ttcgcgagac gcgcagccag cgcgtcgtat tcggcgacgc tgccgaccgg
     5521 gcctatccgc acgcggtgca gcgtgcgacc gccgacttca cccgaccgca cgaaggcatt
     5581 gccgatgccg ccctcgcgca gccgctcgac gaggcgccgt gcattgccgc cgtcggcaaa
     5641 ggcgcccgcc tggatgtaca gaccctcgcc cgccgtcggc gcgagactgg tcgcggcagg
     5701 cgtcgcgagc ggcgccggcg ccacggcgac cgcggtgccg ggcgtgatga cctgcacttc
     5761 gacgaaggcg gtgcctttct gcagcatgtc gagcttcgcg gcggccgtgt aggacaggtc
     5821 gatgatgcgg ccggccacga acgggccgcg atcgttgatc cgcaccacga cgctgcggcc
     5881 gttggcgagg ttggtgacgc gcgcgtagca cgggatcggc agcgtcttgt gcgccgcggt
     5941 catcgcgtac atgtcgtagg gctcgcccat cgaggtgagt gcggcatgga agcccggccc
     6001 gtaccaggag gcgacgcccc gctcgatata gccctcggcg ctcggcagca ccacgtagcg
     6061 ctgcccgaag acttcgtaga agggggggtt gccgcgcagg ctgcgcggct cggcacgcgg
     6121 cacggcatcc ggaatcgcgg ccaccgaagc cggcggtgcg ggcgtggctg caggtaccgg
     6181 cgcgcgggcg ggcgcgctct tcgtgtcgcg gctgccgcaa ccggcgagga ccagcgggag
     6241 cagggcgaga gccgctgccg gaaggactat gcgcctcact cgcctctctc gaacacttcc
     6301 gcggcgacgg cctgcgcgag gtcgtgcacg gccatcgcat agcgcacgct gcggttgtag
     6361 cgcgtgatgg tgacgaagtt cgcgaagccg atccgcacgc cggtgccgtg ttcggtttcg
     6421 gcggggatca ggatcacctt gctgtcgcca ggcagcgtcg actcgaacac gaggcccttg
     6481 gcgcgcgccg aatcgagcgt gtcggtcagc gacaggttgc gcgggtcgag ctcgagcctg
     6541 cgaccctcgg ccagccgcgc ctcgatcagc accgggccgc ccacctgcca gtcgtgttcg
     6601 cggaagtagt tggcgatgct cgccaccacg tcgtcccagc tggcgaacag gtcgcggcgg
     6661 ccgtcaccgt cgccgtcgac ggcgtagcgc cggtagctcg agggcatgaa ctgcggcgcg
     6721 cccatggcgc ccgcatacga gccgagcgcg gtcgttggat cgacgctctc ttcgcgcgag
     6781 agcagcagga actgggtcag ctccttgctg aagaacggcg atcgtggcgg atagtcgaac
     6841 gccagcgtgg cgagcgcatc gagtacgcga tagttgccgg tgatgcggcc gtaataggtc
     6901 tcgacgccga ggatcgagac gatgtactcg ggcgccacgc cgcgttcggc gctgatgcgc
     6961 tcgagcagtt cgcggtgttg ctgccagaag gccactccct cgcgcacgcg ctgtggggtc
     7021 aggaagcgct tgcggtattc ccaccacggc gagactttct cggccggccg ctccatcgcc
     7081 tcgatgatct tcggcttcgg ttcggccttg gcgagcaggc cgtagaaatc ctcgggcgcg
     7141 atcccttcac tggccgcggc ggactcgacg tactggcgga tgtcggcacg ggcgagatcg
     7201 aaacggtcgg tgccggcgaa cacgccatcc gcgtgggcgg cgggaagcgc gaggcaggca
     7261 agggcgaggg cggtggcgag tgtcctcaag agcggatcag tttccgatgt gaatagagcg
     7321 acatcagaat accgaagccc gccatcaggg tcaccatcga agtgccgccg taactgacga
     7381 gcggcagcgg tacgccgacg accggcagca ggccgctgac catgcccgtg ttgatgaaga
     7441 cgtagacgaa gaacgtgatc gcgatgctgc cggacagaag gcgcgcaaag gtctcctgcg
     7501 tctgcatcgc aaggaacatc gcccggccga cgacgaacag gtacaggaat aacagcagcg
     7561 cgaggccgac gaggccgaat tcctcgccga tcacggcgaa aatgaagtcg gtggagcgtt
     7621 cgggcaggaa ttcgagctgg ccctggctgc cgttcatcca gcccttgccg aagatgccgc
     7681 ccgagccgat cgcgatctgc gactggatga tgtggtagcc ggcgccgagc gggtcggatt
     7741 gcgggtcgag gaacatcagc acgcgcttct gctggtactc gtgcaggaag ctccagccga
     7801 accaggcgcc gatcgcggcg agccccccga gcgcgaggat gacgcgcagc tgcagccccg
     7861 ccatcaggat cacgaggaca ccggcgatcg tgatcagcag gccggtgccg aggtccggct
     7921 gcgcgatcgt cagcgccgcc ggcacgaata tgatcatcac ggtgaccagc aggtggggcg
     7981 tcgatggcgg cagtggtcgc tcgtgcaggt accacgcgca catcatcggc accgcgatct
     8041 tcatgaactc cgagggctgg aagcggatca ggccgagatc gagccagcgc tgcgcgccct
     8101 tgccgacata gcccaccacg tcgacgacga tgagcagcac gacgcccgcc acatagatga
     8161 tcggcgtggc ccggcgcagg aacccgggct tgacctgcgc gacgagcagc atcacgacaa
     8221 tcccgatgcc gaggcgcgtg gcggacttga tcaccgcgcc gacgttctgg ccggaggcgc
     8281 tgtacatgac gatcaggcca tagagcgcga tcagcgcgag cccgatgagc agcggtccgt
     8341 cgagcttcag gctcgcgagc gcgcgcgccg agcgggtcag tgtgcggcgc gtgtaggagc
     8401 tgtcgatgct gccgaaggtc aagctccgcc ccccttcagc agccaggcat ccatgacctt
     8461 gcgcgcaatc ggtgcggcgc cggtggagcc gaagccgcca ttctcgacga gcacggcgac
     8521 ggcgatgcgc ggcgcttccg ccggcgcgaa cgcgatgaac caggaatgat cgcgcaggcg
     8581 ttcggccacg gtcttctcgt cgtacttctc ggtctggccg acggagaaca cctgcgccgt
     8641 gccggtcttg cccgcgatcg tgtaggtcat gttgcggccg gcggtcgccg ccgccgtgcc
     8701 gcggccccgc attgcaccga tcatgccctg ggtcacgata tcccactgct cgggcgtcgc
     8761 gctgatcgcg gcgaccttct gcggcgcgag ttgccgcacg gcgccgctga ccggatcccg
     8821 cagcgcggtg acgagccgcg gcttgtacga ggtgccgtgg ttggccagga tcgaggtgta
     8881 gtgcgcgagc tgcagcggcg tgacgaggaa gtagccctgg ccgatgccga aattgaccgt
     8941 ctcgcctgga aaccacacct ggtccgccgg atcggagaac gccttgcgct tccactcgcg
     9001 cgagggcagc aggccaggct tttcgccgcc gatatcgatg cccgtcaggc tgccgaagcc
     9061 gaagggcgcg aggaagcccg agatgcgatc gacgccgagg cggctcgcga gatcgtagaa
     9121 gtacacgtca caggagcgcg cgatcgaatc gacgagggcg accgcgccgt gccggccgct
     9181 gcgcccttcg cggaacatgc ggctgctgcc gggcaggcgg aagacaccgt tgcagaagcg
     9241 cgtttcggtc gggatcacct cgccatgaac gaggccggcg agggcgatcg ccggcttgat
     9301 ggtcgagccg gacggatagg tgccgcgcag tgcgcggttg aacagcggcc ggtcgatgtc
     9361 gtcgagcagc gcctggtact cgacgcgcga gatgccgcga ccgaaggcat tggggtcgta
     9421 gccgggccgg ctcaccagtg cgatcacgtc gccgttggcg ggatcgatcg cgatgaccgc
     9481 gcctcgccgc gcaccgagtt cggcctcggc gacccgctgc acctcgatgt ccatcgcgag
     9541 gaccaggtcc ttgcccgcga ccggctggac ggtgatgagg ttggggacga gtgcgccctg
     9601 gcgctgcacc gagcgaccct gcgcattcac gaggatttcg cgcgagccgt tctgcccgtg
     9661 caactcgctc tcgtaggccg actcggcgcc cagcttgccg atcagtgtcg taccggaata
     9721 ggccgcccgg tcgatgcgcc cgaggtcctg ctcgctgatc gcgcccacgt agccgagcgc
     9781 gtgcacagcc agttcgccgt aggggtacga gcgggcgagg cgcgtgcgga tgtcgacgcc
     9841 ctggaactcg aagcgatgca cggcgaaggt ggcgatctgt tcatcgtcga ggcgcaggcg
     9901 gatcggcacg ctgtcgaagc tgcggtgcga gcgcaccagc ttgcgcgtgt cgtcgatgtc
     9961 gtcggccgcg atcaggccga tctcgctcag gcgccgcagg gtcgcgtcga gatcgggcac
    10021 ttcctcgcgc acgagctcga gctggtaggc cgggcggttc tcggcaagca cggtgccgtt
    10081 gcgatcgagg atcagcccgc gtggtgccgg cagcggctcg atgcgcacgc ggttgccctg
    10141 cgacagatcg gtgtagtact cgtgccgcac gacctgcagc gtgacgaggc gggcgaacag
    10201 cacgagggcc agcacgccga tgatcgcgcc ggcggccatc gcgcgccgct cgaacagccg
    10261 ctgttccttc cagtggtcct tgatgcgtac gttggccggc atggccgctc agcgcgggcg
    10321 atgcgtgcgc gacagcacgc cgaagaccac cggccagacc aggccgccgg tgaccgggtg
    10381 cagccagcgc agcggcgagt tgagcgtgtg cccggtccag ccgtcgatgc accagacgac
    10441 gaattcatag agcatgagcg cggcgaacac gatcagcgcc tgctcgaaca gcggcttggc
    10501 gcgaatcagc aggtggaagc gcatcatcag gaaggcgacg atggtcagtg cgagcgcgtg
    10561 ctcgccgagc acggcgccgc ggaacacgtc gagagcaagg ccgcagacga aagggaacag
    10621 caggccgccg gcgcgcggcg cgacgatcga ccagtagagc accacgagca cgaggaacgc
    10681 cgggcgcgcg atcgcgagcc attccggcaa tggcacgacc tggaagatga gcgcgatcag
    10741 tgccgacagc agcaacgggc cgcgcggttc tctgacccgc acgttcatgg ggcggcctcg
    10801 ggggacggaa ggaccgtgga ttccacggcc ggcgcggcgg gatggcccgc gcggaaccac
    10861 acgagcatca cctcgcgatc gcgatcaatc gcggcgagcg gttgcgcgtg cacctgtgcg
    10921 agcggctggc cggggtcgcg gccgacatcg aggacgcgtg ccaccggata acctgccggg
    10981 aagacgccac caaggcccga ggtcacgagc aggtcgtcga ccttgatgtc ggagttgacc
    11041 ggcagatagg gcagggtcat gacgccggtg ttgccctggc cgacggcgat cgtgcgcagg
    11101 ccgctgcgcg cgacctgcac cggcacggca tgttcggggt cggaaatcag gatgacctcg
    11161 gcggaccagg gtccgacgcg catcacctgg cccatcaggc ctccgcgcgc gagcaccgac
    11221 tgccccttga acacgccatc ggcggcgccc ttgttgacga ccacgcgctg gcgcagcgtg
    11281 ctgagctcga cggcgagcac ttcggcgacc agccagcgct cggccaggtc gggcagcgcg
    11341 gcctcgagtt cgcgcaactg cgcgttctcg gcctcgagcg attcgatgcg cagcgcgcgc
    11401 agttcgagcg ccttgttcgc ttcgcgcagc gcggcgttct cggcggcgag ccgctcgcgc
    11461 gagacgaaat tgtcgcgcac ccagtgccag gctgccgacg gggagttgac tgcgagctgc
    11521 accggatagg cggccgccga cagcgcgtaa cgggcgtatt cgagccagtt gccgcgctgg
    11581 tcgaggaaca tcagcacgat cgccagggcg acgtagaaag tgaagcgcgg gcccgtcgca
    11641 gcgacacggc tgtggacggc acgacctgac ccgaatatcg tcacgcgcga ttgcccctgc
    11701 gcgcggccag aatcactcca gcccgaagtg gcccgggccc atctcgtcca tcagttcgag
    11761 aatgcgccct ccgccacgcg ccacgcaggt cagcggatca tcggcgatca gcaccggcag
    11821 gccggtctcc tccatcagca gcttgtcgat gtcgcgcagc aatgcgccgc caccggtcag
    11881 cacgatgccg cgctcggcga cgtcggcgcc gagttcgggc ggagtctgtt cgagcgcctg
    11941 cttgaccgcg ctgacgatgc cctgcagcgg ctcctgcagc gcctcgagaa tttcgttgct
    12001 gttcagcgtg aacgcgcgcg gcacgccttc cgagaggttg cggcccttga ccgacagctc
    12061 gcgcacctgc tggcccgggt aggccgagcc gatctcgatc ttgatgcgct cggcggt
//
LOCUS       DAOQVY010000070        12070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_5964_length_12070_cov_5.340241, whole genome shotgun sequence.
ACCESSION   DAOQVY010000070 DAOQVY010000000
VERSION     DAOQVY010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12070
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5964_length_12070_cov_5.340241"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PKN91_07795"
     CDS             <1..202
                     /locus_tag="PKN91_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28012.1"
                     /translation="FDLDDEFLGETPAEDGTEYDRMLDKVDKRSRTQPGKRGKAAWSR
                     LEEVLADRRLEKDLRDIFEDDA"
     gene            275..2866
                     /locus_tag="PKN91_07800"
     CDS             275..2866
                     /locus_tag="PKN91_07800"
                     /EC_number="2.7.2.4"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF006515.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/diaminopimelate
                     decarboxylase"
                     /protein_id="HNS28013.1"
                     /translation="MMTTQVDAPWIVLKFGGTSVSTAPNWQRIAQIVRRRAADGSRVL
                     LVHSALSGITDRLAALLEAALQGTHAEIIADIEARHHALAADLGVAVPEGLQRNLAEL
                     RQLASGVALVGEVSDRTRARVMATGELMATELGAAYLRAQGLDVVWADARRMLRAAGR
                     EGASARARVLSATCDFAPDAALARDLGSGAPVCITQGFIASDSDGHTVLLGRGGSDTS
                     AAYFAAKLGAQRLEIWTDVPGMFSANPRTTPAARLLKSLHYDEAQEIATSGAKVLHPR
                     CLLPVRQYGIPLSVHDTRHPDLVGTLISAQARDAAQVKAVCVKKGITLVSLDSPGMWH
                     QVGFLADAFQVFKAQGLSVDLVSTSETNVTVSLDPAAITLDGDVVSALVEALSELCRV
                     RVIGPCASVSLVGRNIRGILHRLGDAFNLFEDQQIYLVSQAANDLNFTFVVDESQADR
                     LVNQLHELLVQPVHADPVLGPTWEQLFAQSDGGEARAVLPWWHEERDALLAALGSRDA
                     AYVYHVTSVERAANSLKALGSVARVWYAMKANCHPELLRRIHAAGLGIECVSRGEIEH
                     VRTHVPGILPEQILYTPNFASRDEYAWALDAGVHLTIDSPYALRHWSALFKGRDLYVR
                     IDSGTGRGHHHHVRTAGAHAKFGVPVEGLAEVAGLAGAAGARIVGLHAHPGSGVFDVE
                     SWRHTALLLAELAARHPDVRTINVGGGLGVPDHSDRPPLDLARLDAVLGEVRSAHPGL
                     EIWLEPGRYLVAAAGVLLARVTQTKQKDEVRYVGIATGMNSLLRPALYGAYHEIVNLT
                     RLDTPATDIVNVVGPICESADVLGHDRLLPPTQEGDVLLLATAGAYGHVMSSSYNLRA
                     PAEELLF"
     gene            complement(2855..3490)
                     /gene="hisIE"
                     /locus_tag="PKN91_07805"
     CDS             complement(2855..3490)
                     /gene="hisIE"
                     /locus_tag="PKN91_07805"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HNS28014.1"
                     /translation="MSFSKADIVTIAFDQRDGLVPAVVQDADGGGVLMLGYMNSAALQ
                     ATLDRQRVVFWSRSRKRLWEKGETSGHTLELVDVLADCDQDTLLVLARPRGPVCHTGC
                     RTCFGERPPGAAERIAFLATLERLVDQRIADRPEGSYTARLHAAGPRRVAQKVGEEGV
                     EVALAGASDEPQALVSEAADLLYHLLVLLRSREVSLDQVITELAARHASKQ"
     gene            complement(3487..4221)
                     /locus_tag="PKN91_07810"
     CDS             complement(3487..4221)
                     /locus_tag="PKN91_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNS28015.1"
                     /translation="MRDGRVVKGVRFRGHEIVGEILELAARYRDEGADEIVFYDITAS
                     PEGRSVDRSWVERVARVLDIPFCVAGGIRSIADAESVLGAGAEKISVNSPALADPALI
                     DRLAARFGSQCVVVGIDSETTANGYRVYALTGDPDRSRDSGRSTLDWVREVQDRGAGE
                     IVLNCMSNDGVRRGYDIAQLAAVRALCDVPLVASGGAGAIGHFTAVFRDAGVDAALAA
                     SVFHSGAIGIAELKAALAAENIEVRQ"
     gene            complement(4236..4976)
                     /locus_tag="PKN91_07815"
     CDS             complement(4236..4976)
                     /locus_tag="PKN91_07815"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNS28016.1"
                     /translation="MLLIPSIDLRGGRCVRLHQGNFDAEKAYDADPAQLLAQYAGYGA
                     RWLHIVDLDGARDGLLANRALVLALAAERRLHLQVGGGVRTCAVVDDLFENGVQRVVI
                     GSAAIERPVDVRDWFRRYGPERLCLAFDVRDADTDAPIVQTRGWVRDSGVSLWRAVES
                     FEPCGLEHVLCTDVARDGALAGPNLGLYAEAQRRFPQIRWQASGGVRDAADLAALAQL
                     GVSAAISGKALLEKRIDPTELQPYFPAA"
     gene            complement(4967..5575)
                     /gene="hisH"
                     /locus_tag="PKN91_07820"
     CDS             complement(4967..5575)
                     /gene="hisH"
                     /locus_tag="PKN91_07820"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNS28017.1"
                     /translation="MSADVAIVDSGGANLASLQFALERLGARGTITASPAEIRSAPRV
                     ILPGVGAAGDAMQRLRSAGLDTVLPGLAQPVLGICLGMQLLYERTEEGDTTCLGIVPA
                     AVHRLHPAPGLPAPHMGWNRVRARRTTGLLDGIADGEHFYFVHGYAASVTPDTIATTD
                     YGSELTAIVQRRNFLGTQFHPERSGTAGARVLQNFLRGASCC"
     gene            complement(5572..6645)
                     /gene="hisB"
                     /locus_tag="PKN91_07825"
     CDS             complement(5572..6645)
                     /gene="hisB"
                     /locus_tag="PKN91_07825"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HNS28018.1"
                     /translation="MLSPQAMLFIDRDGTLIEEPPDEQVDSLEKVKFLPGVFAALAAL
                     REHGYHLAMVTNQDGLGTPSFPQASFELPQRFVIDTFASQGVHFDAVFVCPHLPRDSC
                     ECRKPRTALVDAYLRERPIDLAASAVIGDRESDLEFAANLGVRGLRVRVDGSADETWP
                     AIVRELTARRARVERSTRETTISVAVDLDATGPVRALTGIGFFDHMLEQIAKHGGFSL
                     ELECQGDLHVDEHHTIEDCALALGAVLRQALGDKRGIGRYGFLLPMDEARAQVAIDLS
                     GRAYAVFEGRFGRDQVGGLPSELVPHFFRSLADSLGASIHVSVTGENTHHMIEACFKG
                     VGRALRQALRREGADLPSTKGVL"
     gene            complement(6639..7700)
                     /gene="hisC"
                     /locus_tag="PKN91_07830"
     CDS             complement(6639..7700)
                     /gene="hisC"
                     /locus_tag="PKN91_07830"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNS28019.1"
                     /translation="MSDPLALARPEIRALEAYEHAAWQPGLARLHANEVPWRGPADRS
                     IAGLNRYPEPHPDALAAQLAALYGVPAQCALACRGSDEAIDLLVRTFCTAGRDAVLVC
                     PPTFGMYAVAARIQGAAVIAVPLARDRSYALDVPAIEARLADGNVRLVFLCSPNNPTG
                     NLMAADDVRRVIAAARGRALVVIDEAYAEFAAGNSWAHIIGAEPHAVILRTLSKAWGL
                     AGARIGTLLADERIVGLLRKVIPPYAIAQPCIEAAEAALAPTQQRVAREWVAAIVAER
                     ERLQAGLAALPCVTRIWPSAANFLLVEFDDAARARARVQHAGLLVRDFSDRPGLGQAL
                     RITVGSPEQNDQLIGSLAC"
     gene            complement(7697..9022)
                     /gene="hisD"
                     /locus_tag="PKN91_07835"
     CDS             complement(7697..9022)
                     /gene="hisD"
                     /locus_tag="PKN91_07835"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNS28020.1"
                     /translation="MTDIPIHDWSGLDAAGRARVLARPHPEVGDTIHAEARAIVDAVR
                     GGGDEAVRRFTSRFDGVPPERLATLAATPAEFAAARGELGAAQIRALERAIANVESFH
                     AAQRPAALSVETMPGVRCERVVRPIGSVGLYVPAGSAPLPSAVIMLAVPARLAGCPQR
                     VLCSAPDRAGRVNAAVLVAAQLCGIDTVFRIGGAQAIAAMAYGTASVPKVDKIFGPGN
                     AWVTAAKQLVAGDPAGAALDLPAGPSEVLVIADGTARADFVAADLLAQAEHDPLAQAV
                     LVTDSRHLAGEVVAAIGRQLPQRSRQRILAASLQSTRIILVEDIRTAIAVSNAYAPEH
                     LLLQVDAPRRWLDAIDCAGSVFVGPWSPEPIGDYCSGANHVLPTYGYARACSGLSLQD
                     FTKQITVQELTAAGLAALGPTASTLAQLEGLDAHAAAVDLRLAALGPGA"
     gene            complement(9019..9918)
                     /gene="hisG"
                     /locus_tag="PKN91_07840"
     CDS             complement(9019..9918)
                     /gene="hisG"
                     /locus_tag="PKN91_07840"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNS28021.1"
                     /translation="MDKDTRLKLAVQKSGRLTDPSLDILARCGLKLSRGKDQLVGFGE
                     NMPLDVLFVRDDDIPDLVQEDVCDLGLVGLNVIEEKRLALAARGVEANFESLRTLDYG
                     RCRLSIAVPEGTPYAGARSLEGKRIATTYPHLLNRFLTSQGVRANVVTLSGAVEIAPR
                     LGRADAICDLVSTGSTLAANHLVEAETVLESHAVLIRTPVALAPVKNDWVERLLMRID
                     GVQQVKESKYIMLHAPRAALPEIRRLLPGSESPTIIPLEGHPDRVAIHAVCRENVFWE
                     TLEGLKAAGASALLVLPVEKMLA"
     gene            complement(9911..10216)
                     /locus_tag="PKN91_07845"
     CDS             complement(9911..10216)
                     /locus_tag="PKN91_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HNS28022.1"
                     /translation="MKLHQNLSARQEAQYERNLYAAVLTLRTPEEMRAFFRDLCTPAE
                     IEAMADRWAVVECLHRGLPYREIHRITGVSITTIGRVARYLTEGNGGYATAAQRLHG"
     regulatory      10321..10433
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            10541..11578
                     /locus_tag="PKN91_07850"
     CDS             10541..11578
                     /locus_tag="PKN91_07850"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF006449.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HNS28023.1"
                     /translation="MEHPERIDPGQAAADTAASDARPRMVMREGVLVIPGEVALYHGG
                     MLQGTSVAWRLEGAEGAPIVAALGGISAHRRVYGDNGWWNGVVGPGCALDAAQLRLLS
                     FDYLGGSGESSGPRPGEAFPRISTYDQADALLRLLNHLGVTALHGIVGASYGGMVALA
                     FAERYPERVGRLIVVSAADRAHPMATAWRSVQRRVMRFAARCGQAGEGVEIARALAMA
                     TYRSPEEFAARFSGAPRTDTEVAVFPVEEYLFARGAEYAARHSTESFLCLSESIDLHR
                     VDTSRIFVPTTAVAVREDQLVPVSDMRRLVARLEHARLHEISSVYGHDAFLKEAALLR
                     PAFADLTGSPA"
     gene            11608..>12070
                     /locus_tag="PKN91_07855"
     CDS             11608..>12070
                     /locus_tag="PKN91_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNS28024.1"
                     /translation="MRAGLETDDATGAVVPPIHLTSTFAFRGFDDKRAYDYTRSGNPT
                     RDLLADALADLEGGAGAVVTATGMAAITVVGHLLPAGARIVAPHDCYGGSFRLFTAWQ
                     RRGELRVDFVDFGDAAAVDAALATPFAMLWIETPSNPLLRISDIAELAARAR"
BASE COUNT         1844 a         4122 c         4260 g         1844 t
ORIGIN      
        1 cttcgatctg gacgacgagt ttctcggtga gactccggcc gaagacggca cggaatatga
       61 ccgcatgctc gacaaggtcg acaagcgttc acgtacgcag ccgggcaagc gtggcaaggc
      121 cgcgtggagc cggctcgagg aagtgctggc cgatcgccgt ctcgagaagg acctgcgcga
      181 catcttcgaa gacgacgcct gacgcccgcc cgggcgccgc gtccacccgg cggtttccgg
      241 tccgcgccat gttacgcggc ccgcagcgag gcacatgatg acgacgcagg tcgatgctcc
      301 ctggatcgtg ctgaagttcg gcggcacgag cgtatccacg gcccccaact ggcagcgcat
      361 tgcgcagatc gtgcgccggc gtgcggccga cggctcgcgc gtgctgctcg tgcactccgc
      421 cctgagcggc atcaccgatc gcctcgcggc actgctcgag gcggcgctgc agggcacgca
      481 cgcggagatc atcgccgaca tcgaggcgcg tcatcacgcg ctcgccgcgg acctgggggt
      541 cgctgtgccg gaaggcctgc agagaaacct ggccgaactg cgccagctcg cctcgggcgt
      601 cgcactcgtg ggcgaagtca gcgaccgtac ccgcgcgcgc gtgatggcta cgggtgaact
      661 gatggcgacg gaactcggcg cggcttacct gcgtgcacag gggctcgatg tcgtatgggc
      721 cgacgcgcgt cgcatgctgc gcgccgccgg gcgcgagggc gccagcgcgc gtgcgcgagt
      781 gttgtccgcg acctgcgatt tcgcgcccga cgctgcgctc gcgcgcgacc tgggcagtgg
      841 tgcgccggtg tgcatcacgc agggcttcat cgcgagcgac tccgacggcc ataccgtgct
      901 cctcggtcgc ggcggttcgg atacttccgc ggcgtatttc gccgccaagc tgggcgcgca
      961 gcggctcgag atctggaccg atgtgccggg catgttcagc gccaacccgc gcaccacgcc
     1021 ggccgcccgg ctgctgaagt cgctgcacta cgacgaggca caggaaatcg ccaccagcgg
     1081 cgcgaaggtc ctgcacccgc gctgcctgct gccggtgcgc cagtacggga tcccgctttc
     1141 cgtgcatgac acgcgccacc cggatctcgt cggcacgctg atcagcgcgc aggcccgcga
     1201 tgccgcgcag gtgaaggccg tctgcgtcaa gaaaggcatc acgctcgtat cgctcgacag
     1261 cccgggcatg tggcaccagg tcggcttcct cgccgacgca tttcaggtct tcaaggcgca
     1321 gggtctctcg gtcgacctcg tgtccacttc ggagacgaac gtgaccgtgt cgctcgaccc
     1381 ggcggcgatt accctggacg gggatgtcgt cagcgcactt gtcgaggcgc tctcggagct
     1441 gtgtcgcgtg agggtcatcg gcccatgcgc atcggtgagt ctcgtcgggc gcaacatccg
     1501 cggcatcctg catcggctcg gcgatgcctt caaccttttc gaggaccagc agatctacct
     1561 cgtcagccag gccgcgaacg acctgaactt caccttcgtc gtcgacgaga gccaggccga
     1621 caggctcgtg aaccagttgc acgaactgct ggtgcagccg gtgcatgccg acccggtgct
     1681 cggcccgacc tgggagcagc tgttcgcgca atccgatggc ggcgaggcgc gggcagttct
     1741 gccatggtgg catgaggagc gcgatgcgct gctcgcggcg ctcggctcac gcgacgcggc
     1801 gtacgtctac cacgtcacca gtgtcgaacg tgccgcgaat tcgctgaagg cgctcgggtc
     1861 ggtcgcgcgc gtctggtatg cgatgaaggc gaactgccat ccggagctgc tgcgacgaat
     1921 ccatgccgcg ggactcggca tcgagtgcgt gtcacgcggc gagatcgagc atgtgcgcac
     1981 gcacgtgccg ggcatcctgc cggaacagat cctgtacacg ccgaatttcg cctcgcgcga
     2041 cgagtacgcc tgggcgctcg atgccggcgt gcatctgacg atcgacagcc cctacgccct
     2101 gcgccactgg tccgcgctct tcaagggtcg cgacctgtac gtgcgtatcg acagcggcac
     2161 gggtcgcggc caccatcacc atgtgcgcac ggcgggcgcg catgcgaagt tcggcgtgcc
     2221 ggtcgaggga ctggccgagg ttgcaggcct ggcgggtgcg gcgggtgcgc gcatcgtcgg
     2281 cctgcatgcg catcccggca gcggcgtgtt cgacgtcgag agctggcggc acacggcgct
     2341 gctgctggcg gaacttgccg cgcgccaccc ggatgtgcgc acgatcaacg tcggcggcgg
     2401 cctcggcgtg ccggaccaca gcgaccggcc gccgctcgat ctcgcgcgcc tcgatgcggt
     2461 actcggcgaa gtgcgcagcg cccacccagg gctcgagatc tggctggaac ccggccgcta
     2521 cctcgtcgcg gcggcgggcg tgctgctcgc gcgcgtgacg cagaccaagc agaaggacga
     2581 agtgcgctat gtcggcatcg cgaccggcat gaattcactg ctgcggcctg ccctgtacgg
     2641 cgcctaccac gagatcgtca acctgacacg gctcgatacg ccggcgaccg atatcgtgaa
     2701 tgtcgtgggt cccatctgcg agagcgcgga cgtgctgggc cacgaccggc tgcttccgcc
     2761 tacgcaggaa ggggatgtgc tgctgctggc taccgccggc gcctatgggc acgtaatgag
     2821 ttcgtcgtac aacctgcgcg cgccggccga ggaactactg ttttgaggcg tgccgggccg
     2881 ccagctcggt gatgacctga tcgagcgata cctcgcgact gcgcagcagc acgagcagat
     2941 ggtagagcag gtcggcggcc tcgctgacca gcgcctgcgg ctcgtcggac gcgccggcga
     3001 gcgccacctc gacgccttcc tcgccgacct tctgcgccac acgcctcggt cccgcggcat
     3061 gcaatcgtgc cgtatagctg ccctcggggc ggtccgcgat gcgctggtcg accagccgct
     3121 cgagcgtcgc gaggaacgcg atccgctcgg cggcacccgg cgggcgctcg ccgaagcacg
     3181 tgcggcaacc cgtgtggcac accggtccgc gcggtcgtgc gagcacgagc agcgtgtcct
     3241 ggtcgcagtc ggcgagcacg tcgaccagct cgagcgtgtg acccgaggtt tcacccttct
     3301 cccacagccg tttgcggctg cggctccaga acacgacgcg ctggcgatcg agcgttgcct
     3361 gcagcgcggc cgaattcatg tagccgagca tcagcacgcc accgccgtcg gcatcctgca
     3421 cgacggccgg cacgaggccg tcgcgctgat cgaaggcgat cgtcacgata tccgctttcg
     3481 agaaactcat tgccgaacct cgatgttctc ggccgccagt gcggccttca gctctgcgat
     3541 gccgatcgcg ccggaatgaa agacactcgc ggcgagagcc gcgtcgacgc ccgcgtcgcg
     3601 gaacacagcg gtgaagtgcc cgatggcgcc ggcgccgccc gaggcgacca gcgggacgtc
     3661 gcacagcgcg cgcacggccg caagctgcgc gatgtcgtag ccgcgacgca cgccatcgtt
     3721 cgacatgcaa ttgagtacga tctcgccggc tccccggtcc tgcacttcgc gtacccaatc
     3781 aagtgtactg cggcccgaat cgcgcgagcg gtccggatcg ccggtgagcg cgtagacgcg
     3841 gtagccattc gcggtggtct cgctgtcgat gccgaccacg acgcattggg agccaaagcg
     3901 tgcggccaga cggtcgatca gtgcgggatc cgcgagtgcg ggggagttca ccgaaatctt
     3961 ctcggcgccg gcgccgagca cggactcggc gtcggcgata ctgcgaatgc cgcccgcgac
     4021 gcagaacggg atgtcgagta cgcgtgcgac gcgctctacc cagctacggt cgaccgagcg
     4081 gccttcgggg ctcgcggtga tgtcatagaa gacgatctca tcggcgccct cgtcgcggta
     4141 gcgcgcggcg agttcgagga tctcgccgac gatctcatgg ccgcggaagc gtacgccttt
     4201 gacgacgcgg ccgtcacgca cgtcgaggca ggggattatg cggcgggaaa gtatggctgc
     4261 agctccgtcg ggtcgatgcg cttctcgagc agggccttgc cgctgatcgc cgcgctgacg
     4321 ccgagttgcg cgagcgcggc gagatcggcg gcgtcgcgta ccccgcctga tgcctgccag
     4381 cggatctgcg ggaagcggcg ctgtgcctcg gcgtacaacc cgaggttcgg gccggcaagg
     4441 gcgccgtcgc gcgcgacgtc cgtgcacagc acgtgctcga gcccgcaggg ctcgaagctc
     4501 tcgaccgcac gccacaggct gacgcccgag tctcgcaccc agccgcgcgt ctgcacgatc
     4561 ggtgcgtcag tgtcggcgtc gcgcacgtcg aatgcgagac acaggcgctc gggtccgtag
     4621 cggcggaacc aatcgcggac gtcgacgggg cgctcgatgg ccgcgctgcc gatcacgacg
     4681 cgttggacac cgttctcgaa caggtcatcg acgaccgcgc acgtgcgcac gccgccgccg
     4741 acctgcaggt gcaggcggcg ttctgcggcg agcgcgagca ccagcgcgcg attggcgagc
     4801 aggccgtcgc gcgcaccgtc gagatcgacg atgtgcagcc agcgcgcgcc gtagccggcg
     4861 tactgcgcca gcagctgcgc gggatcggcg tcataggcct tctcggcgtc gaagttgccc
     4921 tggtgcaggc gcacgcagcg gccgccgcgc aggtcgatcg aggggatcag cagcatgatg
     4981 ctccacggag gaaattctgc aggacacggg cgccggccgt gccggagcgc tcgggatgaa
     5041 actgcgtgcc gaggaaattg cgtcgctgca cgatggcagt gagctcgctg ccgtagtcgg
     5101 tcgtcgcgat cgtatcgggg gtcaccgacg cggcgtagcc gtgcacgaag tagaaatgct
     5161 cgccatcggc gataccgtcg aggaggccag tcgtgcggcg cgcgcggacc cggttccatc
     5221 ccatgtgcgg cgcgggcagt ccgggcgcgg gatgcaggcg atgcaccgcg gccggcacga
     5281 tgccgaggca ggttgtgtcg ccctcctcgg tgcgctcgta gagcagctgc atgccgaggc
     5341 agatgccgag taccggctgg gcgagtccgg gcaggaccgt gtcgagtccc gcgctgcgca
     5401 ggcgttgcat cgcatccccg gcggcgccca ctcccggcag gatcacccgc ggggcactgc
     5461 gtatctcggc ggggctcgcc gtgatcgtgc cgcgtgcccc gaggcgctcg agcgcgaact
     5521 gcagcgacgc aagattggcc ccgccgctgt cgacgatcgc gacatcggcg ctcacagcac
     5581 tcccttcgtg ctcggcaggt cggcaccctc gcggcgcagc gcctggcgca gcgcgcggcc
     5641 gacacccttg aagcaggcct cgatcatgtg gtgcgtgttc tcgccggtca cgctgacgtg
     5701 gatcgaggcg cccaggctgt cggccagcga acggaagaaa tgcggcacca gttcgctcgg
     5761 caggccaccg acctggtcgc ggccgaaacg gccttcgaag accgcgtacg cgcggcccga
     5821 caggtcgatc gcgacctgcg cgcgcgcttc gtccatcggc agcaggaacc cgtagcggcc
     5881 gatgccacgc ttgtcgccga gcgcctggcg cagcaccgcg ccgagtgcga gtgcgcaatc
     5941 ctcgatcgtg tggtgttcgt ccacgtgcag gtctccctgg cactcgagct cgagcgagaa
     6001 gccgccgtgc ttggcgatct gttcgagcat gtggtcgaag aagccgatcc cggtcaaggc
     6061 gcgtaccggc ccggtggcgt cgagatcgac ggcgacgctg atcgtggtct cgcgcgtgct
     6121 gcgctcgacg cgtgcccgcc gcgccgtgag ttcgcgcacg atcgcgggcc acgtttcgtc
     6181 tgcgctgccg tcgacgcgca cgcgcaggcc gcgcacgccg aggttggccg cgaattcgag
     6241 atcgctctcg cgatcaccga tcaccgcact tgccgcgagg tcgatcggcc gctcgcgcag
     6301 gtaggcgtcg accagcgcgg tgcgcggctt gcgacactca caggagtcgc gcggcaggtg
     6361 cggacagacg aacaccgcgt cgaagtggac accctggctg gcgaaggtgt cgatcacgaa
     6421 gcgctgcggc aactcgaaac tcgcctgcgg aaagctcggc gtgccgaggc cgtcctggtt
     6481 cgtcaccatc gcaaggtggt agccgtgttc gcgcagcgcg gcgagcgccg cgaacacgcc
     6541 gggcaggaac ttcacttttt cgagcgagtc gacctgctcg tcgggcggct cctcgatcag
     6601 cgtgccgtcg cggtcgatga agagcatggc ctgagggctc agcatgcgag actcccgatc
     6661 aattggtcat tctgctccgg gctgccaacc gtgatgcgca gcgcctggcc gagtccgggg
     6721 cgatcgctga aatcgcgcac aaggagtccc gcgtgttgca cacgtgcccg ggcgcgggcc
     6781 gcatcgtcga attcgaccag caggaagttg gcggcgctcg gccagatcct cgtgacacag
     6841 ggcagcgcgg cgaggcccgc ctggagccgc tcgcgttccg cgacgatcgc cgccacccac
     6901 tcgcgcgcga cccgctgctg cgtcggtgca agcgccgcct cggccgcttc gatgcagggt
     6961 tgcgcgatgg catagggggg aatcaccttg cgcagcaggc cgactatgcg ctcgtcggcg
     7021 agcagcgtgc cgatgcgcgc accagcgagc ccccaggctt tcgacagcgt gcgcaggatg
     7081 acggcatgcg gctcggcccc gatgatgtgt gcccagctgt tcccggccgc gaactcggca
     7141 tacgcctcgt cgattacgac cagcgcccgc ccgcgcgccg ccgcgatgac acgtcgcacg
     7201 tcgtccgcgg ccatcaggtt gccggtcggg ttgttggggg agcagaggaa cacgagtctg
     7261 acgttgccgt ccgcgagtcg cgcctcgatc gcggggacat cgagcgcgta gctcctgtcc
     7321 ctggcgagcg gcacggcgat gaccgccgca ccctggatgc gtgcggcgac cgcgtacatg
     7381 ccgaaggtcg gcgggcagac cagcacggcg tcgcgaccgg cggtgcagaa ggtgcgcacc
     7441 agcaggtcga tcgcctcgtc actgcctcgg caggcgaggg cgcactgcgc cggcacgccg
     7501 tacagggccg cgagctgcgc cgccagcgca tcggggtgcg gctccggata gcgattgagt
     7561 cccgcaatcg agcgatcggc cggcccgcgc cacggcactt cattggcgtg cagccgcgcg
     7621 aggcccggct gccacgcggc gtgctcatac gcctcgagcg cgcggatttc gggacgcgcg
     7681 agtgcgagcg ggtcgctcat gcgccgggac ccagcgcggc gaggcgcaga tcgacggcgg
     7741 cggcatgagc atcgaggcct tcgagctgcg ccagcgtcga tgcggtgggc ccgagcgccg
     7801 cgagaccggc ggccgtcagc tcctgcacgg tgatctgctt ggtgaaatcc tgcagcgaca
     7861 gtccgctgca tgcgcgcgca tagccgtagg tgggcagcac gtgattggca ccagagcagt
     7921 agtcgccgat cggctcgggc gaccagggcc cgacgaacac cgagccggcg cagtcgattg
     7981 cgtcgagcca gcgccgcggc gcatcgacct gcagcaacag gtgctccggc gcgtaggcat
     8041 tcgatacggc gattgcggtg cggatgtcct cgaccaggat gatgcgcgtg ctctgcaggg
     8101 aagccgcgag gatacgctgg cgcgatcgtt gcggcagttg cctgccgatt gcggcgacga
     8161 cttccccggc gagatgccgt gaatcggtga ccagcacggc ctgtgcgagc gggtcatgct
     8221 cggcctgcgc gagcaggtcg gcggcgacga agtccgcgcg ggcagttccg tcggcaatca
     8281 ccagcacctc cgaggggccg gccggcaggt cgagcgcggc gccggcggga tcgcccgcga
     8341 cgagttgctt ggccgccgtc acccaggcgt tgccaggccc gaagatcttg tcgaccttgg
     8401 gcacgctcgc cgtgccgtag gccatggccg cgatcgcctg agcgccaccg atgcggaata
     8461 ccgtgtcgat gccgcacaac tgcgcggcga ccagcacggc cgcattcacg cggccagcgc
     8521 gatcgggcgc actgcacagc acgcgttgcg ggcagcccgc aaggcgcgca ggcacggcca
     8581 gcatgatgac cgccgacggc aggggtgcgg accccgccgg cacatacagg ccgaccgagc
     8641 cgatcgggcg tacgacccgt tcgcagcgca cgcccggcat cgtctcgacc gacagcgccg
     8701 cggggcgttg cgccgcatgg aagctctcga cgttggcgat ggcgcgctcg agtgccctga
     8761 tctgcgccgc gccgagttcg ccacgggcgg ccgcgaattc cgccggcgtc gcggcgagtg
     8821 tcgcgaggcg ctcgggcggc acgccatcga agcgcgaggt gaaacggcgc acggcctcgt
     8881 cgccgccgcc gcgcacggca tcgacgatcg cacgcgcctc cgcgtgtatc gtgtcgccca
     8941 cctccgggtg cggtcgcgcg agcacccgcg cgcgacccgc ggcatccagc ccggaccagt
     9001 catggatcgg gatgtccgtc acgcgagcat cttctccacc ggcagcacga gcagggcgct
     9061 cgcgccggcc gccttcagtc cctcgagcgt ttcccagaac acgttctcgc ggcacactgc
     9121 gtggatcgcc acccggtcgg ggtgaccctc gagcgggatg atggtcggcg actcgctccc
     9181 cggcagcagc cggcggatct ccggcagtgc cgcgcgcggc gcgtgcagca tgatgtactt
     9241 gctttccttc acctgctgca cgccgtcgat acgcatcagc aggcgctcga cccagtcgtt
     9301 cttcacgggt gcgagggcga ccggagtgcg gatcaggacc gcgtgactct cgagcaccgt
     9361 ctcggcttcg acgaggtgat tcgcggccag cgtggagccg gtcgacacga ggtcgcagat
     9421 cgcgtcggca cggccgagac gcggagcgat ctcgacggcg cccgatagtg tgacgacgtt
     9481 cgctcgcacg ccctggctgg tcaggaagcg gttgagcagg tgcggatagg tggtcgcgat
     9541 gcgcttgccc tcgagcgagc gagcgcccgc gtagggcgtg ccctccggca ccgcgatgga
     9601 caggcggcag cgaccgtagt cgagcgtgcg cagcgattcg aagtttgcct cgacgccgcg
     9661 cgcggcgagc gcgaggcgct tctcctcgat gacgttgagg cccacgagcc cgaggtcgca
     9721 gacgtcctcc tgcacgagat cggggatgtc gtcgtcgcgc acgaacagca cgtcgagcgg
     9781 catgttctcg ccgaagccga cgagctggtc cttgccacgc gagagcttca ggccgcagcg
     9841 cgccaggatg tcgagcgacg ggtcggtgag gcgcccggac ttctgcaccg cgagtttgag
     9901 gcgcgtatcc ttatccatgc aggcgctgcg cggcggtggc gtagccgccg ttgccctcgg
     9961 tcaggtagcg cgcgacgcgc ccgatcgtcg tgatgctgac cccggtgatg cgatggatct
    10021 cccgataggg caggccgcgg tgcaggcact cgaccaccgc ccagcggtcg gccattgctt
    10081 ctatctcggc cggggtgcac aggtcacgaa agaaggcgcg catctcctcg ggcgtacgca
    10141 aggtgagcac ggcggcataa agattgcgct cgtactgggc ttcctgcctg gcggaaaggt
    10201 tctggtgcag tttcatggcc tgtgctaatg tattaacgtg ttagcacgat agcacatgcc
    10261 gggcggcgat ggaagcgggc gtccttgacc gcttgcagag gcaaccccta gactgcgcgg
    10321 cactcatcga gaccggctga gggacaggcc cttggacgcc ggggcaacca gcgaacccaa
    10381 cccgttcgcc cggtgccaac tcctgcggtc gcgccagcgc cgtgagatga gcggtcggca
    10441 gagccggcag gtcgcaggac ccgccggatg gatgccggac accccgctag ctgcgggtgc
    10501 tcgccgcagg agtgccgatg cgtggaaaga ggcactcgag atggagcatc cggaaagaat
    10561 cgatccaggc caggccgccg cagacactgc cgcctccgac gcgcgcccgc gcatggtgat
    10621 gcgcgagggg gtgctggtga tcccgggcga ggtggcgctg taccacggcg gtatgctgca
    10681 gggcaccagc gtcgcctggc ggctcgaggg tgcggaaggc gcgccgatcg tcgccgcttt
    10741 gggcggcatt tcggcccatc gacgggtcta cggcgacaat ggctggtgga acggcgtggt
    10801 gggcccgggt tgcgcactcg atgcggcgca gctgcggtta ctgtcgttcg actatctcgg
    10861 cggcagcggt gaatcgagcg gtccgcgccc cggggaagcc tttcctcgca tctccaccta
    10921 tgaccaggcc gacgcgttgc tgcggctgct gaatcacctc ggcgtgacgg cgttgcacgg
    10981 gatcgtcggc gcctcctacg gcggaatggt ggcgctcgcc ttcgcggagc gctatcccga
    11041 gcgcgtcggg cggctgatcg tcgtcagtgc cgccgaccgg gcacacccga tggcgaccgc
    11101 ctggcgcagc gtgcagcggc gggtgatgcg ctttgcggcg cgctgcggcc aggcgggcga
    11161 gggcgtcgag attgcgcgcg cactggcgat ggcgacgtac cgcagccccg aagagttcgc
    11221 cgcgcgcttt agcggtgcac cgcgtaccga taccgaggtc gccgtgtttc cggtcgagga
    11281 gtacctgttc gcgcgcggcg cggagtacgc cgcgcgccat tcgaccgaat cgttcctgtg
    11341 cctgtcggag tcgatcgacc tgcatcgcgt cgacaccagc cggatcttcg tgccgaccac
    11401 cgcggtcgcc gtgcgcgagg accagctggt gccggtgagc gacatgcgtc gtctcgttgc
    11461 gcgcctcgag cacgcgcggc tgcacgagat ctcctccgtg tacggccatg atgcctttct
    11521 caaggaggcc gcactgctgc ggcccgcgtt cgcagatctc accgggagtc cagcgtgacc
    11581 gatcgctttg cagaaaatac ccgcgcagtg cgcgcaggcc tcgaaaccga cgacgccacg
    11641 ggcgcagtcg tgccgcccat ccatctgacg tcgacgttcg catttcgcgg ctttgacgac
    11701 aagcgcgcgt atgactacac gcgatccggc aatccgaccc gtgacctgct ggccgacgca
    11761 ctcgcggacc tggaaggcgg cgcgggcgcc gtcgtcacgg cgaccggcat ggcggcgatc
    11821 accgtcgtcg gccacctgct acctgcgggt gcgcgcatcg tcgcaccgca cgactgctac
    11881 ggtggcagct tccgcctgtt cacggcctgg caacggcgcg gcgagctgcg cgtcgacttc
    11941 gtcgatttcg gcgatgccgc cgcggtcgat gccgcgctcg ccacgccctt tgcgatgctg
    12001 tggatcgaga cgccgagcaa tccgctgctg cgcatctccg acatcgccga actcgcggcg
    12061 cgagctcgcc
//
LOCUS       DAOQVY010000071        11496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_6334_length_11496_cov_6.257058, whole genome shotgun sequence.
ACCESSION   DAOQVY010000071 DAOQVY010000000
VERSION     DAOQVY010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11496
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6334_length_11496_cov_6.257058"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /locus_tag="PKN91_07860"
     CDS             complement(<1..1652)
                     /locus_tag="PKN91_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNS28025.1"
                     /translation="MEKYWLNSYPKDVPTEIDPSQYASLKELAEKCCAKYADRDAYVM
                     MDRAMSFGELERESRAFAAWLQQEAGAQRGTRIALMMPNLLQYPVAMFGALRAGLTIV
                     NVNPLYTWHELEHQVNDSGAEIIVVLENFAHVVQKALPSTTLKTVVVTSVGEMLGGLK
                     STIVNFVVRRMQKKVPAWDIPGAHRLGQVLARGRGLTMQPVEVGPDDIAFLQYTGGTT
                     GVSKGAVLTHRNMVANVLQGTAWFGSSMPERATYVIALPLYHIFSLTANALMFLLLGG
                     RGILITDPRDLKTFVKTLQKYPPNLFMGVNTLFNGLLHTPGFAELDHSSLAGTVGGGM
                     AVQAAVAEKWKATTGCVLSQGWGLTETSPIATMNPMGKDYNGSIGLPVPSTGISIRND
                     AGTALGIGEIGEICVRGPQVMRGYWNRPAETAEVMLPDGWLRTGDIGRMDEAGFVYIE
                     DRKKDMILVSGFNVYPNEVEGIAAMHPGVLEAAAVAKPDERSGEAVALFVVRKDPALT
                     EEALISFMRESLTAYKVPKFVYFRNELPKTNVGKILRRTLRD"
     gene            complement(1665..2351)
                     /locus_tag="PKN91_07865"
     CDS             complement(1665..2351)
                     /locus_tag="PKN91_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HNS28026.1"
                     /translation="MVNGPSGLVTCLVMFSALRTMSRVVIGRALIVQCLLLMPFVAQA
                     AERTILVFGDSISAGYGLKAEESWVSLLQKRLVSQGYGYRVVNASVSGETTSGGIARL
                     PRALDLHSPQIVVIELGANDGLRGLPLDLSRRSLAGMIEAAQARGSAVLLLGMRVPPN
                     YGARYAESFHQMYAELARQYDVALVPFFMEKVALEPGLMQADGLHPTAAAQTLLLDTA
                     WPALRRLITG"
     gene            2353..2949
                     /locus_tag="PKN91_07870"
     CDS             2353..2949
                     /locus_tag="PKN91_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS28027.1"
                     /translation="MRDVSLEIVAGESVAIVGPSGAGKSTLLALLAGLDTPTSGRVWL
                     AGQDLTALDEDGRARLRAERVGFVFQAFHLVPALTALENVSLPLELAGRADARRLATA
                     TLDRVGLSARTGHYPRQLSGGEQQRVALARAFVTRPAVLFADEPTGNLDATNGTRVCD
                     LLFELNRDLHTTIVLVTHDHALAGRCTRALHMDDGRIV"
     gene            2961..5456
                     /locus_tag="PKN91_07875"
     CDS             2961..5456
                     /locus_tag="PKN91_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28028.1"
                     /translation="MRAVLIAVRTLAREWRSGELGVLLLALTIAVAALSAVGFVVSRV
                     SAAVELQASEVLAADLRLVSANPLPEAYIAEARRRGLATGSSVGMLSIVFHGETSQLS
                     ALHAVGPAYPLRGTVVIATESFGTPQDAAVGVPPRGEVWPESRLLAAIGAEVGDEVSI
                     GAASFRVGRALVTRPDQGSSFAELAPSLLLNVEDLPATQLIQPGSRVRHAQLFAGDRD
                     DVDAFKAWIGENKQPGERVLDITESSPQVRDATDRAGRFLSLASLVAVLLCSIAVAMA
                     ARRYVQRHLDSVALMKTLGATRAFTLTVSLVQIGLLALIAAAIGAAAGFVAQAWLVRA
                     LRGLLAVASLPPPSLVPIVMAVVTAFAVLAGFALPPLLQLSRVPAIRVLRRDVGPPPL
                     VIWLAFWPAVIAIVGLIYWVVRDVPLLLGFTGGLAVFVAVLALFGVLLVRLTGSLRSR
                     VGVAWRYGIANLARRRAESVVQVVAFGLGIMVLLLLALVRRDLLEDWRASLPADVPNF
                     FFVNIPSADREAFLSFIDGAGAEASRALPMIRGRLTTINDRPLEDMSFNNEGGEGLAN
                     REQNLTWASELGDDNQIVAGRWWGPQDTGQPLVSIATEFRDSVGLKLGDRLTFDIAGE
                     TFEVTVASIRQVKWDSFQPNFFLVFPPGLLDATAGTWMTSAYFAPGKPRPIAQLVRRF
                     PSVSVFDMDNILGQVRGVIDKAAVAVQSVFVFTLLAGLTVLLAAVQASRDERRFESAM
                     LRTLGASRRTVRAGVLAEFITLGLLAGLLAALGASVGGYLIAKHVLEVKYTFDAWVWA
                     WGLGAGTALVAGGGWLATRSVVDQSPLLTLRGG"
     gene            5456..7075
                     /locus_tag="PKN91_07880"
     CDS             5456..7075
                     /locus_tag="PKN91_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28029.1"
                     /translation="MEPARKAAKMVGWFDPRVLMRTAIHMTTANIFGRHSDPRLIEAL
                     ASQPQGHFDYAATDGAGVWLDYVADTGDGWNPVHAIADALARPTLDVAPAGGGAAERT
                     RSGQVLMFGGDEVYPWPSREAYAWRTEWPYQDAFEAHGTSPDIFAIPGNHDWFDSLVA
                     FSRTFCRPERGFAGCRTQQTRSYFALRLPADWWLVAIDLQLGSELDEPQVQYLKRVAA
                     AMEPQAQVILCVPEPQWIFERAYPGHAAYDGNMLATLAGDILQRPVRVFLSGDLHHYR
                     RHENPAGVQKIISGGGGAFLHPTHAPRPRDLPGGFVERAVYPDRTTSRRLAWRNLAFP
                     WLNPHGLVLPAVLYTLSAWFASASLSPADIETLAGTVAASLTAALRDPVDGLWLLTVI
                     GGFVFFTDTHVRWYRLIGGISHAIAHLAAALALAWLAAMFTTGVLGLGFGTIAQLLIA
                     GALTFVSGAVVGGVLIGLYLLVSVQLFGRHANEAFSSLRIEDYKQWLRMHFALDGTLT
                     LYAIGIDRVSRGRGDRRATPPHLIEKLVLRR"
     gene            complement(7088..7939)
                     /locus_tag="PKN91_07885"
     CDS             complement(7088..7939)
                     /locus_tag="PKN91_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28030.1"
                     /translation="MDAATQMLPDVPLTDAPPPRRAAGPSQSPAAAPNAAQTTGQGAQ
                     GAAWAAPAMDRNFIERNQIVERYLSGRLPPRGALEFERFCTEHPDLLDALGLPARVNA
                     GLRLLEVGGKPEPWAEKTLPLWQRPPVVIGALALALVLLIVSAVLASQLGQRSDEIAA
                     LETRVAEQPLAPATSTRTIKLVPARGGPTSSPAVVVGAGTVQFADFKIDIAWSKFDAF
                     RITIDRAGQGRVAVLHNMKKDSNGHLRLAFNTSALGPGNYDLTIQGINWRGEPVAQAW
                     ATIGVQR"
     gene            8068..9597
                     /locus_tag="PKN91_07890"
     CDS             8068..9597
                     /locus_tag="PKN91_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28031.1"
                     /translation="MHKKTFSRTLLAVGLTVAAGAHAADTGGTAITIYSSAQPGAVPV
                     EFYRPVPGGGTPGGMAVPGYAMVRDTRPMTLAAGRSTVRFTDVAALIDPTTVTFQSLT
                     DPGTRVLEQNYQFDLIGMDKLLQRHIDKPVTVDRAGSAAAGPATSGTLLSAGDGLVLR
                     SSDGSIHALREYGAVHFAELPGGLITRPTLVWEVNAARGGEHRTRVTYQTAGITWWAD
                     YNLIFTEGRDANSGTVDLGAWVSIVNQSGASYQDARLKLIAGDVHRVAPPQRVYLQKR
                     AMEMAAAADSAAGFEEKAFFEFHLYTLGRRTALADNSTKQIELFEQTKSIPAKKLLVY
                     YGAPAYAAYGGPYTERDIGTAANTKVDVYLEFRNDKARGLGVPLPAGRVRVSKLDSAD
                     DSLEFIGEDVIDHTPKDEDVRIKLGSAFDVVGERRQVDFSIDTKARVMEEEIEVKLRN
                     HKDQPVEVLVRETLYRWSDWSVVSQTQPFTKEDARTIHFPVRIAKDGETRVRYRVRYR
                     W"
     gene            9687..>11496
                     /locus_tag="PKN91_07895"
     CDS             9687..>11496
                     /locus_tag="PKN91_07895"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNS28032.1"
                     /translation="MTFTRMALHNPAGVAVGALLACLLGVLALFRLPVQLFPDIDEPV
                     ISIATPWRAAAPSEVESEIIEPQERALEGLAGLKELNSFAAPGGAFLNLRFAIGTDMQ
                     ATLIEVISRMNQLPPMPRDAGAPRIALGDDGGAGTNQTLSFFFVQLLPGTPGPVEDYR
                     RAVEELVRARIETIPGVATVQVNAGAPEELQIIFDPQRAAELGIELPRVAAVAGNATD
                     MSGGFVDIGRRQYTMRFAGRYTPEELAGLVLEWRDGRPVRLGDIAAVTVRRGDRTDLA
                     MQNGNPAIGLRVLKENGANVLDTLNAVKAEFDLLRDGPLMAMGLTVAQSFDPSLFINQ
                     ALAMVSGNLVAGVILAVLVLWFFLRDLRTTLLVGIVIPVCLLVALAVLMLTGRTLNVI
                     SIAGLAFGVGMTTDAAVVVIESIAQRREQGVPMLQAALEGTTRVWPALFASTAATIVV
                     FMPVVFMQDAAGQLFADLSLTVVIAVATSLLVAVTMLPLLASRWLKPLAANATSDARW
                     RHMAETLVRWTDTPRRRYAIIGALLIVPASLTWLLLPPLDYLPPVKRAAIDGYFQFPP
                     GASIDTIDNEIVQVMAQRMAPYMSGERKPALRNYYVL"
BASE COUNT         1700 a         3828 c         3955 g         2013 t
ORIGIN      
        1 tcatccctca gcgtccgcct gagaatcttc ccgacatttg tcttcggtag ctcgttgcgg
       61 aagtacacga acttcggcac cttgtaggcg gtcagcgatt cgcgcatgaa cgagatcagc
      121 gcctcctcgg tgagtgcggg gtccttgcgc accacgaaga gtgcgaccgc ttcgccggaa
      181 cgctcatccg gcttcgctac ggccgcggcc tcgagcacgc ccgggtgcat cgcggcgata
      241 ccctcgactt cgttcgggta aacgttgaat ccggacacga ggatcatgtc tttcttgcga
      301 tcctcgatgt agacaaagcc cgcttcgtcc atgcgcccga tgtcgcccgt gcgcagccac
      361 ccgtccggca gcatgacctc cgcggtttct gccgggcggt tccagtagcc gcgcatgacc
      421 tgcgggccgc gcacgcagat ctcaccgatc tcgccgatcc cgagcgcagt gccggcgtcg
      481 ttgcggatgg atattcccgt ggagggcacc ggcaggccga tcgagccgtt gtagtccttg
      541 cccatgggat tcatcgttgc gatcggcgag gtctccgtga ggccccagcc ctggctgagt
      601 acgcagcccg tcgtggcttt ccacttctcg gccaccgcgg cctgcactgc catgcccccg
      661 cccacagtgc cggcgaggct gctgtgatcc agttccgcga agcccggcgt gtgcagcagt
      721 ccgttgaaca gtgtattgac gcccatgaag agatttggcg ggtacttctg cagtgtcttc
      781 acgaaggtct tcaggtcacg cgggtcggtg atcaggatgc cgcgcccgcc gagcagcagg
      841 aacatgagcg cgttcgccgt cagcgaaaaa atgtggtaca ggggcagcgc gatgacatag
      901 gtcgcgcgct cgggcatcga gctgccgaac caagccgtgc cttgcagcac gttggcgacc
      961 atgttccggt gggtgagcac ggcgccctta gagacgccgg tcgtgcctcc cgtgtactgc
     1021 aggaaggcga tgtcatcggg acccacctct accggctgca tcgtcaggcc gcgaccccgg
     1081 gccagcacct ggccgaggcg gtgggcgccc gggatgtccc atgcgggcac tttcttctgc
     1141 atgcggcgca cgacgaagtt gacgatcgtg ctcttcaggc cgccgagcat ttcgccgacc
     1201 gaggtgacca cgacggtctt gagggtcgtg cttggcagcg ccttctgcac cacgtgcgcg
     1261 aagttctcga gcacgacgat gatctcggcg cccgagtcat tgacctggtg ctcgagctcg
     1321 tgccaggtgt agaggggatt tacgttgacg atcgtgagcc ccgcgcgcag cgcgccgaac
     1381 atggcgaccg ggtactgcag caggttcggc atcatcagtg caatgcgggt gccgcgctgc
     1441 gcaccggctt cctgctgcag ccacgcggcg aacgcgcgcg attcgcgctc cagttcaccg
     1501 aagctcattg cacggtccat cattacatag gcgtcgcgat cggcgtactt cgcgcagcac
     1561 ttctcggcga gttccttgag tgacgcgtac tggctcgggt cgatctcggt cggaacgtcc
     1621 ttcggatatg aattcagcca gtacttttcc aaggcaatct ccgttcagcc ggttatcagg
     1681 cggcgcagcg cgggccaggc cgtgtcgagc agcagcgtct gtgccgcggc ggtgggatgc
     1741 aggccgtcgg cctgcatcaa tccgggttcg agcgccacct tctccatgaa gaatggcacc
     1801 agtgccacgt catactgcct ggccagctcg gcatacattt ggtgaaagct ttcggcgtag
     1861 cgggccccat agttcggcgg cacacgcatg ccgagcagca gcaccgcgga gcctcgcgcc
     1921 tgtgcggcct cgatcatgcc tgcgaggctg cgacgtgaca gatcgagcgg caggccgcgc
     1981 aggccgtcat tggcgccgag ttcgatcacg acgatctgag gtgagtgcag gtcgagcgcg
     2041 cgcggcaggc gggcaatgcc accgctcgtc gtttcgccgc tcacgctggc gttcacgacg
     2101 cggtacccgt acccttggct gacaagccgt ttctggagga gggataccca actctcctcg
     2161 gctttgagac cgtagcccgc gctgatgctg tcgccgaaca cgaggatcgt gcgctcggcc
     2221 gcctgcgcga cgaatggcat caggagcaga cactggacga tgagcgcccg cccgatgact
     2281 actcgagaca ttgttctcaa agctgaaaac atcaccaagc aggttaccag ccccgaaggg
     2341 ccgttgacca ttgtgcgcga cgtttcgctc gagatcgtgg ccggcgaatc ggtggcgatc
     2401 gtggggccgt ccggtgccgg aaaatcgacg ctgctcgcgc tgctggcggg tctcgatact
     2461 ccgacttccg gacgtgtttg gctcgccggt caggacctca cggcgctcga cgaggacggt
     2521 cgcgccaggt tgcgtgcgga gcgcgtcgga ttcgtattcc aggccttcca cctggtgccc
     2581 gcgctcaccg cgctcgagaa tgtctcgctg ccgctcgagc tcgcgggccg agccgatgcg
     2641 cgccggctcg cgaccgccac gctcgaccgg gtcggcctga gtgcgcgcac cggccactat
     2701 ccgcgccagc tctcgggagg tgagcagcag cgcgttgcac tcgcgcgtgc cttcgtgacg
     2761 cgtcccgcgg tgctgtttgc ggacgaaccg accggcaacc tcgatgcaac gaacggcacc
     2821 cgtgtctgcg acctgctctt tgaactcaat cgcgacctgc acacgacgat agtgctcgtc
     2881 acgcacgacc acgcgctcgc cgggcgctgc acgcgcgcgc tgcacatgga cgacgggcgg
     2941 atcgtctgag ctcccgggtc atgcgcgccg tcctcatcgc tgttcgcacc ctggcccgtg
     3001 agtggcgctc gggcgaactg ggcgtgctgc tcctcgccct cacgatcgcc gtggcggcgc
     3061 tttcggcagt cggcttcgtc gtgagccgag tcagtgcggc ggtggaattg caggcgagcg
     3121 aggtgctcgc cgccgacctg cggctcgtgt cggccaatcc gctgccggag gcctatatcg
     3181 cggaggcgcg gcggcgcggc ctcgccacgg ggagcagcgt aggcatgttg tcgatcgtgt
     3241 tccacggcga gacttcgcag ctctcggcgc tgcacgccgt gggtccggcc tacccgttgc
     3301 gcggcacggt ggtcatcgcc accgaatcgt tcggtacccc gcaggacgcc gcggtcggtg
     3361 tgccgccgcg cggcgaggtg tggccggaga gccgcctgct tgccgcgatc ggcgcggagg
     3421 tgggcgatga agtgtcgatc ggtgcggcga gctttcgggt cggacgcgcg ctcgtcacgc
     3481 ggcccgacca gggttcctcg ttcgcggagc tcgcaccgtc actgctgctg aacgtcgagg
     3541 atctgccggc gacccagctg atccagccgg gcagccgggt tcgccatgcg cagctgttcg
     3601 ccggcgatcg tgacgacgtg gacgcgttca aggcctggat cggtgagaac aagcagcccg
     3661 gcgagcgcgt gctcgacatc accgagtcga gtccgcaggt ccgcgatgcg accgaccggg
     3721 ccggccgctt cctgagcctg gcgagcctcg tcgcggtgct gctgtgctcg atcgcggtcg
     3781 ccatggcggc gcgccgctat gtgcagcgcc atctcgattc cgtggcgctg atgaagacac
     3841 tcggtgcgac ccgcgccttc acgctcacgg tgagcctcgt gcagatcggc ctgctggcgc
     3901 tgatcgccgc ggccatcgga gcggcggcgg gtttcgttgc gcaggcgtgg ctggtgcgcg
     3961 cgctgcgcgg tctgctggca gtcgcgagcc ttccgccgcc cagcctggtg ccgatcgtga
     4021 tggcagtggt cacggcgttc gcagtactcg ccgggttcgc cttgccgccg ctgctgcagc
     4081 tctcgcgcgt gcccgcgatc cgcgtgctgc gccgggatgt cggcccgccg ccgctcgtca
     4141 tctggctcgc gttctggccg gccgtgatcg ccatcgtcgg gctcatttac tgggtggtgc
     4201 gcgatgtgcc gctgttgctc ggcttcacgg gaggcctggc ggtcttcgtg gcggtgctgg
     4261 cgttgtttgg cgtgctgctc gtgcgtctca cggggtcgct ccgcagccgg gtcggcgtcg
     4321 cctggcgcta cggtatcgcc aacctcgcac gtcgccgcgc cgaaagcgtc gtgcaggtcg
     4381 tggccttcgg tctcggcatc atggtgttgc tgctgctcgc gctggtgcgg cgcgacctgc
     4441 tcgaggactg gcgcgcgagc ctgccggcgg atgtgcccaa cttcttcttc gtcaatatcc
     4501 cgtccgccga tcgcgaggcg ttcctctcgt tcatcgacgg cgcgggcgcc gaggcttcac
     4561 gcgcgttgcc gatgatccgc ggtcgcctga cgacgatcaa cgaccggccg ctcgaggaca
     4621 tgtcgttcaa caacgagggc ggcgaggggc ttgcgaatcg cgagcagaac ctgacctggg
     4681 cgagcgaact cggcgatgac aaccagatcg tcgccggtcg ctggtgggga ccgcaggaca
     4741 ccggccagcc gctcgtgtcg atcgcgaccg agtttcgcga ttccgtgggc ctgaagctcg
     4801 gcgaccggct cacattcgac attgcgggcg agaccttcga agtcacggtc gccagcatcc
     4861 gccaggtgaa gtgggacagc ttccagccga acttcttcct ggtgtttccg ccgggcctgc
     4921 tcgatgcgac cgcaggtacc tggatgacga gcgcgtactt cgcgcccggc aagccacggc
     4981 cgatcgcgca gctcgtgcga cgcttcccga gcgtgtcggt attcgatatg gacaacattc
     5041 tcggacaggt ccgcggggtg atcgacaagg cggcggtcgc ggtgcagagc gtgttcgtct
     5101 tcaccctgct cgcgggactg accgtgttgc tcgcggcggt gcaggcaagt cgcgacgagc
     5161 ggcgtttcga gagcgccatg ttgcgcacgc tcggcgcaag ccggcgcacc gtgcgtgcgg
     5221 gtgtgctggc cgagttcatc acgctcgggt tgctcgccgg tctgctcgct gcgctcggcg
     5281 cgagcgtcgg cggttacctg atcgcgaaac acgtgctgga agtgaagtac acgttcgacg
     5341 cgtgggtgtg ggcctggggg ctcggtgccg ggaccgccct ggtggcgggt ggcggctggc
     5401 tcgccacgcg ctcggtggtg gaccagtcgc ccctcctgac tctgcggggt ggctgatgga
     5461 gcctgcgcgc aaggccgcca agatggtggg ctggttcgat ccgcgcgtgc tgatgcggac
     5521 cgcgatccac atgacgacgg ccaacatctt cggccgtcat tccgacccgc gcctgatcga
     5581 ggcgctggcc agccagccgc agggccactt cgactatgcg gccaccgatg gcgcgggcgt
     5641 gtggctcgac tacgtcgccg ataccggtga cggctggaac ccggtgcatg cgattgccga
     5701 cgcactcgcg cgccccacgc tcgatgtcgc gccggccggc gggggagccg ccgaacggac
     5761 ccgatccggc caggtgctca tgttcggtgg cgacgaggtg tatccctggc cctcgcgaga
     5821 ggcctacgcc tggcgcactg agtggccgta ccaggacgcc ttcgaggcgc atggtacgag
     5881 ccccgacatt ttcgcgatcc cgggcaacca tgactggttc gacagtctgg ttgccttctc
     5941 gcgcacgttc tgccggccgg agcgtggctt cgccggctgc cgcacgcaac agacacgcag
     6001 ctattttgcg ctgcgcctgc cggccgactg gtggctggtc gcaatcgacc tgcagctcgg
     6061 ctccgaactc gatgaaccgc aggtgcagta cctgaagcgt gtcgctgcgg cgatggagcc
     6121 gcaggcgcag gtgatcctct gtgtgccgga gccgcagtgg attttcgaga gggcctaccc
     6181 cggacacgcg gcgtatgacg gcaacatgct cgcaacgctc gccggcgaca tcctgcagcg
     6241 tccggtgcgc gtgttcctgt ccggcgatct gcatcattac cggcggcacg agaaccccgc
     6301 gggcgtgcag aagatcatct ccggtggcgg cggcgccttc ctgcatccca cccacgcgcc
     6361 gcggccgcgg gacctgccgg gcgggttcgt ggaacgcgcc gtgtatcccg atcgcacgac
     6421 ctcgcgacga ctcgcgtggc gcaatctcgc tttcccgtgg ctcaatccgc acggcctggt
     6481 gttgcccgcg gtgctctaca cgctgtccgc atggttcgcg tcggcgagcc tgtcgcccgc
     6541 ggacatcgag accttggcgg ggaccgtcgc ggccagtctg acggctgcgc tgcgcgatcc
     6601 ggtggatgga ctctggctgc tcaccgtcat cggggggttc gtgttcttta ctgacacgca
     6661 tgtgcgctgg taccgcctga tcggcggcat ttcgcacgcg atcgcgcacc tcgcggccgc
     6721 gcttgcgctc gcgtggctcg cggcgatgtt cacgaccggg gtgctcgggc ttggcttcgg
     6781 caccatcgcg cagctgctga tcgccggggc gctgaccttc gtttccggcg cagttgtcgg
     6841 tggtgtgctg atcggcctgt acctgctggt gtcggtgcag ctgttcgggc gccacgcgaa
     6901 cgaagcgttc tcgtcgctgc gcatcgaaga ctacaagcag tggctcagga tgcactttgc
     6961 gctggacggc acattgacgc tctatgcgat cggcatcgat cgcgtgtcgc gcgggcgcgg
     7021 ggacaggcgc gcgacgccgc cccacctgat cgagaaactc gtcctcaggc gctgaccggc
     7081 acgccgctca gcgctgcacg ccgattgtcg cccaggcctg cgccacgggc tcgccacgcc
     7141 agttgatgcc ctggatcgtc aggtcgtagt tgccgggccc gagcgccgag gtgttgaagg
     7201 cgaggcgcag gtggccgttc gaatccttct tcatgttatg caggaccgcg acacggccct
     7261 gtccggcacg gtcgatcgtg atccggaaag cgtcgaactt cgaccaggcg atgtcgatct
     7321 tgaagtcggc gaactgaacg gtgccggcac cgaccaccac cgcaggcgaa ctcgtcggcc
     7381 cgccacgcgc cggcacgagc ttgatcgtgc gcgtggaggt cgcgggcgcg aggggctgct
     7441 ccgcgacccg cgtttcgagt gcggcgattt cgtcgctgcg ctggccaagc tgcgatgcga
     7501 gcaccgccga gacgatgagc agcacgagcg cgagtgccag ggcaccgatc acgaccggcg
     7561 gccgctgcca gagggggagc gtcttctcgg cccagggctc gggcttgccg cccacctcga
     7621 gcagccgcaa tccggcattg acgcgcgcgg gcaggccgag cgcgtcgagc aggtcggggt
     7681 gttcggtgca gaagcgttcg aactcgaggg caccacgggg cggcaggcgc cccgagagat
     7741 agcgctcgac gatctggttg cgctcgatga agttgcggtc catcgccgga gcggcccatg
     7801 cggctccctg ggcgccctgg ccggtggtct gggcggcatt cggggcggcg gcgggggact
     7861 gggaggggcc ggcggcacgg cgcggtggcg gcgcgtccgt cagcggcacg tccggcagca
     7921 tctgcgtggc ggcgtccata gccccatcgt gccttcccgc ccgttcacac gcatggcact
     7981 gctcacagtt ctgttaaccc gcccgaaaag cgcgacatgg gtcgcatttt cgggaaccat
     8041 taccgcccgc gccggaggat ctgccccatg cacaagaaga cgttttcccg gaccctgctt
     8101 gccgtcggac tcaccgtcgc tgccggcgcg cacgccgccg atacgggcgg caccgcgatc
     8161 accatctaca gctcggccca gcccggcgcc gtgccggtcg agttctatcg gccggttccc
     8221 ggtggtggca ccccgggcgg gatggcggtg ccgggctacg ccatggtgcg cgacacgcgt
     8281 ccgatgacgc ttgcggcggg gcgatcgacc gtgcgtttca cggacgtggc ggcactgatc
     8341 gatccgacca ccgtgacctt ccagtcgctt actgaccccg gcacccgggt gctcgagcag
     8401 aactaccagt tcgacctgat cggtatggac aagctgctgc agcgtcatat cgacaagccg
     8461 gtcaccgtcg atcgggccgg tagcgccgcg gcgggtccgg ccacgagcgg cacgctactg
     8521 tcggcaggcg acggcctggt gctgcgcagc agcgacggct cgatccacgc gctgcgcgag
     8581 tacggcgcag tgcatttcgc ggaattgccg ggcggactga tcacgcgccc gacgctcgtg
     8641 tgggaagtaa atgccgcgcg cggcggcgag caccgcacgc gggtcaccta ccagaccgcg
     8701 ggcattacct ggtgggccga ctacaacctg atcttcactg aagggcgcga cgccaacagc
     8761 ggcaccgtcg atctcggtgc gtgggtcagc atcgtcaacc agtcgggcgc gagctaccag
     8821 gacgcgcgcc tgaagctgat cgcgggcgac gtgcaccgcg tcgcgccccc gcagcgcgtg
     8881 tacctgcaga agcgcgcgat ggaaatggcc gcggccgccg attccgccgc cggattcgag
     8941 gagaaggcgt tcttcgaatt ccatctctat acgctcgggc gtcgcacggc tctagcggac
     9001 aactcgacca agcagatcga gctgttcgag cagacgaagt cgattccggc gaagaagctc
     9061 ctcgtctact acggcgcgcc ggcctatgcg gcgtacggcg gaccgtacac cgagcgcgac
     9121 atcggcacgg cggccaacac gaaggtcgac gtttacctcg agttccgtaa cgacaaggcc
     9181 aggggtctcg gcgtgccgct gcccgccggg cgggtgcgtg tgtcgaagct cgacagcgcc
     9241 gacgacagcc tggagttcat cggcgaggac gtgatcgatc acacgccgaa ggacgaggac
     9301 gtgcgcatca agctgggttc ggccttcgat gtcgtgggcg agcgccgcca ggtcgatttc
     9361 tctatcgaca ccaaggcccg cgtcatggaa gaggagatcg aggtgaagtt gcgcaaccac
     9421 aaggaccagc cagtcgaagt cctggtgcgc gagactctct atcgctggag cgactggtcg
     9481 gtggtttcgc agacccagcc gttcacgaaa gaggacgccc ggacgatcca cttcccggtg
     9541 cggatcgcga aggacggtga aacgagggtg cgctaccggg tacgttaccg ctggtagcga
     9601 cgagccgtgc cagtccagcg atgagccgct ggcactgcgc ggccggatga ccatccaacg
     9661 ggggacaatt gcgtcaggga ggacgcatga ccttcaccag gatggcgcta cataacccgg
     9721 cgggtgttgc ggtcggggca ctgctcgcct gcctgctcgg cgtgctggcg ctgttcaggc
     9781 tgccggttca gctgtttccg gacatcgacg agccggtcat cagtatcgcc acgccctggc
     9841 gcgccgcggc gccgagcgaa gtggaatccg agatcatcga gccgcaggag cgtgctctcg
     9901 agggtcttgc cgggctgaag gaactcaaca gcttcgcggc gccgggcggc gcgttcctca
     9961 atctgcgctt tgcgatcggc acggacatgc aggcgacgct gatcgaagtg atcagccgca
    10021 tgaaccagct cccgccgatg ccgcgcgacg ccggcgcgcc gcgcatcgcg ctcggcgacg
    10081 atggcggtgc cggcaccaac cagacgctgt cgttcttctt cgtgcagctg ctgccgggca
    10141 cgccgggtcc ggtcgaggac taccgccgcg cggtcgagga gctggtgcgc gcgcgcatcg
    10201 agacgatccc cggcgtcgcc accgtgcagg tgaatgccgg cgcgccggag gaactgcaga
    10261 tcatcttcga cccgcagcgc gccgccgaac tcggcatcga gcttccgcgg gtcgccgccg
    10321 tggccggcaa tgccaccgac atgtccggcg gtttcgtcga catcggccgt cgccagtaca
    10381 cgatgcgctt tgccgggcgc tatacgcccg aggaactcgc cggcctcgtg ctcgaatggc
    10441 gtgatggacg cccggtgcgg ctcggtgaca tcgccgcggt gaccgtgcgg cgcggcgatc
    10501 gcaccgacct cgcgatgcag aacggcaacc cggcaatcgg gctgcgcgtc ctgaaggaga
    10561 acggcgcgaa cgtcctcgac acgctgaatg cggtcaaggc cgagttcgac ctgctgcgcg
    10621 acggcccgct gatggccatg ggcctcacgg tggcgcagtc gttcgatccc tcgctgttca
    10681 tcaaccaggc gctggccatg gtgagcggca atctggtggc gggcgtgatc ctcgcggtgc
    10741 tcgtgctgtg gttcttcctg cgcgacctgc gcaccacact gctcgtcggc atcgtgatcc
    10801 cggtgtgcct gctggtcgcg ctcgcggtgc tgatgctgac cgggcgcacg ctcaacgtaa
    10861 tctcgatcgc cggcctcgca ttcggtgtcg gcatgacgac cgacgctgcg gtggtggtga
    10921 tcgagagcat cgcgcagcgg cgcgagcagg gtgtgccgat gctgcaggcg gcgctcgagg
    10981 gcacgacgcg cgtctggccg gcgctgttcg cctccacggc cgcgaccatc gtcgtgttca
    11041 tgccggtggt gttcatgcag gatgcggcgg gacagctgtt cgcggatctc tcactcaccg
    11101 tggtaatcgc ggtggccacc tcgctgctcg tcgccgtcac gatgctgccg ctcctcgcct
    11161 cgcgctggct caagccgctc gccgcgaacg cgacgtcgga tgcgcgctgg cggcacatgg
    11221 ccgagacgct ggtgcgctgg accgatacgc cgcgacgccg ctacgcgatc atcggggcgc
    11281 tgctcatcgt gccggccagc ctcacctggc tgctgctgcc gccgctcgat tacctcccgc
    11341 cggtgaagcg cgcggcgatc gacggctact tccagtttcc gcccggcgcg agcatcgaca
    11401 cgatagacaa cgagatcgtg caggtgatgg cgcagcgcat ggcgccctac atgagcggcg
    11461 agcggaagcc ggcgctgcgc aactactatg tgctgg
//
LOCUS       DAOQVY010000072        11303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_6473_length_11303_cov_5.324413, whole genome shotgun sequence.
ACCESSION   DAOQVY010000072 DAOQVY010000000
VERSION     DAOQVY010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11303
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6473_length_11303_cov_5.324413"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PKN91_07900"
     CDS             complement(<1..628)
                     /locus_tag="PKN91_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS28033.1"
                     /translation="MIPVARLAGVTLRYKDVTALDVPRLELPAGAVVGLIGPDGVGKS
                     SLLSLVAGARALQDGELEVLGGNMRDRQFRRDLHPRIAYLPQGLGRNLYPTLSVHENV
                     DFFGGLFGLPTTERRRRIGALLEATELAEFANRPAAKLSGGMKQKLGLCCALIHDPDL
                     LILDEPTTGVDPLSRRRFWELVEGIRASRPGMSVLVATAYMEEAARFDW"
     gene            complement(625..1704)
                     /locus_tag="PKN91_07905"
     CDS             complement(625..1704)
                     /locus_tag="PKN91_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNS28034.1"
                     /translation="MSIAPLVQRLLRIAIFLMVLAAVGYVLWQRFGASDADAGIASGN
                     GRIEAVEIDVATKSAGRLLELLAKEGEFVTTGQVLARMDTESLRAQLQQAEAQARQAE
                     ATLETARSQVAQRDSERAAAQALVKQRQAELDAEQTRAQRITALAQRGDVSRQSADDA
                     RTAVETRSGALATARAQLAAAEAAIVSARSQVAAAASAIEAARATVARVQTEIDDSEL
                     KAPRDGRVQVVVARPGEVLGAGGRVLNLVDLTDVYMTFYLPTGVAGRIALGAEARVVL
                     DAAPEYVVPARISYVADVAQFTPKTVETAAEREKLMFRVRAQIPQDLLRRYIHEVKTG
                     LPGVAYVRTDPSLSWPEHLQVKLPQ"
     gene            complement(1789..2508)
                     /locus_tag="PKN91_07910"
     CDS             complement(1789..2508)
                     /locus_tag="PKN91_07910"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNS28035.1"
                     /translation="MFREHRDPGQFAQHRAVWRRAAVLVGLCVGLAVFASAENLHEAL
                     LEVLAVAQTVVDRHPIAGTLLFVAFAAVSAMFTFVSIAVLVPAAVYVWGEPGSMALLW
                     LGWILGGAATYGIGTWFGRPVVRWVTSNPALLRFEHRVQQNAPFWLILLLQLALPSEV
                     PGYVLGLARYPFLRYLLALAIAELPYTVATVYLGTSFLERRRGVILGAGLAIALLSVV
                     AFYLLRRVLKSAGENAGNPSV"
     gene            2643..3605
                     /locus_tag="PKN91_07915"
     CDS             2643..3605
                     /locus_tag="PKN91_07915"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HNS28036.1"
                     /translation="MTAGDPADAGANTSGLRGVERLIAHVRDMEPVRMAVVHPCDALS
                     LGGAMDARAAGMIIPVLVAPEAKLRRVATDAGIDLAGCEIVDVPHSHAAAARAAQMAC
                     RGEVEALMKGSLHTDELLGAIVAPEAGLRTGRRITHCFVMETAAYPRPFIITDAAVNI
                     APDLEAKADIVRNAIDLAHVIGVALPKVAILAAVETVNPRMPSTLDAAALCKMADRGQ
                     ITGGLLDGPLAFDNAVSPDAARVKGIRSAVAGVADILVVPDLESGNMLVKQLEYMGHA
                     DSAGIVMGARVPIVLTSRADSRRSRLASCAIALMLAHRFRVAPP"
     gene            3618..4808
                     /locus_tag="PKN91_07920"
     CDS             3618..4808
                     /locus_tag="PKN91_07920"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015879521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HNS28037.1"
                     /translation="MTPLILVLNCGSSSIKFALFDAGRRPLARQPLWSGKIEGITGTH
                     ATCRETGLAPVSVALDAARPYDAALAHIRERVRAHIASEGRIAAVAHRVVHGGSRYFE
                     PVRLDAAVLADLKSYVPLAPLHQPFALEAIESLFSLAPGLPQVACFDTAFHRTQEPVE
                     QMFALPWSAWERGVRRYGFHGLSYEYMTHALAERYGDAARGRTLVAHLGSGASLCAMS
                     NLASVATSMGFSALDGLMMGTRCGTLDPGAVIYLMQIEKLTLDEVASMLYYDSGLLGV
                     SGVSADPRELLPLEADNERVRIALALYVRRLVREIGALTALLGGLDLLVFTAGIGEHN
                     AVLRARVCEQLGYLGVRLDAAANARHAAVISDRDSAVTVGIEPTNEEWIAASHAARLP
                     GLVP"
     gene            4987..6111
                     /locus_tag="PKN91_07925"
     CDS             4987..6111
                     /locus_tag="PKN91_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28038.1"
                     /translation="MSARPVLQRLLLSLGLGFLLLASAEGVAQQAAPGALEVFVRDGC
                     PHCDAAKSFLVDFAAERPDLRIVLRPVEDPRTRDELVEHFRRAGAWPPAVPTFVYEGR
                     MLVGFDNPEITGAAVRALVGPLELPPRDEVPTILGGLSAAKLGLPLFTLSLGLLDGFN
                     PCAMWVLLFLLSLLVRLRDRRRMAIIAGTFVLVSGAVYYAFMAAWLNLFLAIGMSNAV
                     RWTLAGIALLIGVVNVKDFVAPGRGVSLSIPEAAKPGLYARARSVLQVETLTASLIGV
                     AMLAVLVNFVELLCTAGLPAIFTAVLAERDLSTGGYYAYLGLYIAGYIADDALMVTLA
                     VLALGNRRLTQRTGRWLKLLSGVLMLALGAILFLRPQWLS"
     gene            6212..6778
                     /locus_tag="PKN91_07930"
     CDS             6212..6778
                     /locus_tag="PKN91_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28039.1"
                     /translation="MNRLHGATRRTALLALLAITCGALSVACTNHEDASPPVVDDATA
                     DHAAHMDHAAQATSGAAIGQLALDAGRKWTTDAPLRAGMAAIHEAFDAEHAAIDAGSM
                     EDARYEALAARIDTHVQDIVRNCKLPPAADANLHYIIADLARGAQEMRGRDPARTRRD
                     GAVLVHGALGAYGEFFDDPEFGAAGELP"
     gene            6903..8987
                     /locus_tag="PKN91_07935"
     CDS             6903..8987
                     /locus_tag="PKN91_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent copper receptor"
                     /protein_id="HNS28040.1"
                     /translation="MRDFPASIAGLLLVGGFMGATQAWAGDTEQEGSTKESALVTVVV
                     TAVAPQSPLTFVTDPKLPRQPVPASDGADYLKTIAGFSAIRNGGTNGDPVLRGMFGSR
                     LNILTDDGSMPGACPSRMDNPLSYVSPETYDRLVVIKGPQTVLWGPGASAGTIRFERD
                     ADADRFEDAGLRVNANALGGTFGRNDQVLDVIGGAGVGYARLSANRSEADDYEDGAGR
                     TVPSSWEKWNADAALGWTPSAGTLLEFSAGTGDGEARYAGRSMDGSQFERTSYGLRFE
                     KTVLGGALKDVKANVFYNHADHVMDNYTLREPNPMSSMPGPFASNVDRRTTGGRVAAS
                     WRLSAIDITAGADTQRSRHRKRNAMGRDAYADQPRQTDAEIAGSGIFAELTWRFGEDS
                     RAVFGGRGDRAEVTDRRQTSGGMMPMPNPTADVTRRETLASGFARYEHGARDRPVSWF
                     AGIGHTARMPDYWELFSPGSGPMGTVNAFAALAPEETTQLDLGLQYRTARVDAWMSAY
                     AGRIDDFILFTYGSGGMMGLMTRVNQVDAAVRGAEAGIELRPLPQWKAAATLAYAWAE
                     NRGTGAALPQIPPLDVRISVAYDNARFSVGALLRAVDGQGRVAPGQGNVAGRDLGRSA
                     GFAVLSLNGAYRLSPRLSLAAGIDNLLDRDYAEHLNLAGSADFGYPAEPVRIHEPGRS
                     AWLKVTWNTGGG"
     gene            9019..10260
                     /locus_tag="PKN91_07940"
     CDS             9019..10260
                     /locus_tag="PKN91_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS28041.1"
                     /translation="MEHPASLSFARLLVNLRWLSVAGQALTILVVTRPLGVALPETPL
                     WAGVGALALFNLFAARRVRTAGDVSVPEVFAHILVDIVALTWMVAWSGGIENPFSSLF
                     LLPIALSILALPGRWVWATALAAMAGYVASALLARELPHVHGVLGDTFSIHKAGMLAN
                     FALSAVVILVFFTRMAAAWRRSEQEVARLREQFARNEGIIALATHAASVAHELNTPLG
                     TLTLMVEDLAAEAGSDAHREDYATMQSLLAVCRERVRDLAAPADAGQAGNRRESVDLE
                     RVIERWQLVRPTIDLRRSGVTSGLGSVDPAVGHLLLALLNNAADAGEQVGAARVDLHI
                     DSGGQGLRVSIRDYGVGFEHAQPVLPAKLFRTNKPGGMGIGLALSHATVERLGGSLSM
                     LAATDGPGVQVSFELPALSTA"
     gene            10257..10817
                     /locus_tag="PKN91_07945"
     CDS             10257..10817
                     /locus_tag="PKN91_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNS28042.1"
                     /translation="MTGKASVAQGPGLLVDDDELYLRTLQRSLQRRGYATVTATSGDD
                     ALAAAKAHRPAYALVDLKLGSMSGLALIAPLRAVRDDMRILLVTGYASIATAVEAIKR
                     GADNYLMKPVTADAIVRALNEETESVPVPEGTMVPLARVEWEHIQQALGETRGNISAA
                     ARLLGMHRRSLQRKLAKRPGPERRGE"
     gene            10938..>11303
                     /locus_tag="PKN91_07950"
     CDS             10938..>11303
                     /locus_tag="PKN91_07950"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS28043.1"
                     /translation="MTRIVVLGAGIGGMSIAYELRGTLGKGPEITVIGQGEHFSFTPS
                     NPWVAVGWREPADVEIAVAGYLAKQHIGFESAGAERVEPADNRVLLRDGRHIEYDYLV
                     IATGPRLAFDEVTGLGPDGG"
BASE COUNT         1809 a         3837 c         3875 g         1782 t
ORIGIN      
        1 gccagtcgaa gcgcgccgcc tcctccatgt aggcggtggc cacgagcacg ctcatgcccg
       61 gccggctggc gcggatgcct tcgacgagtt cccagaagcg acgacgcgag agcgggtcca
      121 cgcccgtcgt gggctcgtcg aggatcaaca ggtccgggtc gtggatcagc gcgcagcaca
      181 ggccgagctt ctgcttcatg ccgccggaca gcttggcggc gggacggttc gcgaattcgg
      241 cgagttccgt ggcctcgagc agcgcgccga tgcggcggcg gcgctcggtt gttggcaggc
      301 cgaagagccc gccgaagaaa tccacgttct cgtggaccga gagcgtcgga tacaggttgc
      361 ggcccaggcc ctgtggcaga taggcgatgc gggggtgaag atcgcggcgg aattgccgat
      421 cgcgcatgtt cccgccgagc acctcgagct caccgtcctg cagcgcgcgg gcgccggcca
      481 cgagcgatag caggctcgac ttgccgacac cgtccggccc gatcagtccg acgacggcgc
      541 cggcgggcaa ctccaggcgc gggacgtcga gcgccgtgac atccttgtaa cggagcgtca
      601 cgccagccag gcgtgccacg ggaatcattg cggcagtttc acctgcaggt gttcgggcca
      661 ggacagggaa ggatcggtgc ggacatacgc cacgcccggc aagcccgtct tcacctcgtg
      721 aatgtatcgg cgcagcagat cctgcgggat ctgtgcgcgc acgcggaaca tcagcttctc
      781 gcgctcagcc gccgtttcga ccgtcttcgg cgtgaactgg gcaacatcgg ccacatagga
      841 gatccgcgcc ggcaccacgt attcgggagc ggcgtccagc acgacgcgtg cctcggcgcc
      901 cagtgcgatc cggcccgcca cgccggtcgg caggtagaaa gtcatgtaga catcggtgag
      961 atcgacgagg ttcagcacgc ggccgccggc cccgagcact tccccgggac gtgcgacgac
     1021 cacctgcaca cgcccgtcgc gcggcgcctt gagttcgctg tcgtcgatct cggtctgcac
     1081 tcgcgcgacg gtcgcgcggg ccgcctcgat ggccgaagcg gccgccgcga cctggctgcg
     1141 ggcgctgacg atcgcggcct cggccgccgc gagctgggcg cgcgcggtgg cgagcgcgcc
     1201 gctgcgcgtt tcgaccgccg tgcgcgcatc gtccgcgctc tgccgcgaga cgtcgccgcg
     1261 ctgtgcgagc gcggtgattc gctgcgcgcg ggtctgctcg gcgtcgagct ccgcctgccg
     1321 ttgtttcacg agcgcctgcg ccgccgcccg ctcgctatcg cgttgggcca cctggctgcg
     1381 cgccgtctcg agggtcgcct cggcctgccg ggcctgggcc tcggcctgct gcagctgtgc
     1441 ccgcagcgac tcggtgtcca tccgcgccag cacctggccg gtggtgacga actccccctc
     1501 cttcgccagc agttccagca gccgtcccgc actcttcgtc gcgacgtcga tttccacggc
     1561 ctcgatgcgc ccgttgccgc tcgcgatccc ggcgtcggca tcgctggcgc cgaagcgttg
     1621 ccacagcaca taacccaccg ccgcaagcac catcaggaag atcgcgatgc gcagcagacg
     1681 ctggaccaga ggggcaatgg acatgcccgg atttgagcag gacgcgcccg ggaagtcgag
     1741 gccaaccctg tcgcaggccc tgtgagacca gcaggcccat caatcagatc agacggaggg
     1801 attgccggcg ttctcgccgg ccgatttcag cacccgccgc agaaggtaga acgcgacgac
     1861 gctgagaagc gcgattgcca gtcccgcgcc gaggatgacg ccccggcggc gctcgaggaa
     1921 actcgtgccg aggtacacgg tggccaccgt gtacggcaat tcagcgatag cgagcgccag
     1981 cagatagcgc aggaagggat agcgcgcgag acccagcaca tagcccggga cttccgaagg
     2041 cagcgcgagt tgcagcagga ggatgagcca gaagggtgca ttctgctgca cgcggtgctc
     2101 gaatcgcagg agcgcaggat tcgacgtaac ccagcgcacc accggccgac cgaaccaggt
     2161 gccgatgccg taggtggcgg caccgcccag gatccatccg agccacagca gcgccatgct
     2221 gccgggctca ccccacacat acaccgcagc aggcaccaga accgcaatcg acacgaaggt
     2281 gaacatggcc gagactgccg cgaatgcgac gaagagcaat gtgcccgcga tcggatgccg
     2341 atcgacgacc gtttgggcga ccgccaggac ttcgagcagc gcctcatgca gattttcggc
     2401 tgacgcgaac accgcgaggc ccacgcagag gcccacgagc acggcggcac ggcgccagac
     2461 ggcgcgatgc tgggcgaact ggccggggtc gcgatgctcg cggaacatcg cgccatgatc
     2521 cgcgcgcggc gcgatcaaat ccagccgctc cggggcgtac ggcgccttcg gcgatcggct
     2581 tgtcgccgac cacgacaatc acgtagcgtg agccgcccgg ggcgatcgag ggaccaggag
     2641 ccatgactgc aggagatccc gccgatgccg gcgcgaatac gtcaggtctg cgcggcgtcg
     2701 aacggctgat cgcgcacgtg cgcgacatgg agcccgtgcg catggcggtg gtgcatccgt
     2761 gcgatgcgct cagcctcggc ggcgccatgg acgcgcgtgc ggccggcatg atcatcccgg
     2821 tgctggttgc gcccgaggcg aagctgcgcc gggtggcgac cgacgcgggc atcgacctcg
     2881 ccggttgcga gatcgtcgac gtgccgcaca gccacgcggc ggccgcgcgg gcggcgcaga
     2941 tggcctgccg gggcgaggtc gaggcgctga tgaagggcag cctgcacacc gacgaactgc
     3001 tcggcgccat cgtcgcgccc gaggcagggc tgcgcaccgg gcggcgcatc acacactgct
     3061 tcgtgatgga gacggcagcc tacccgcgcc ccttcatcat caccgatgcg gccgtcaaca
     3121 tcgcgcctga cctggaggcg aaagccgaca tcgtgcgcaa cgcgatcgac ctcgcgcacg
     3181 tgatcggcgt cgcgctgccg aaggtggcga ttctggccgc ggtagagacc gtcaatcccc
     3241 gcatgccatc gacactggac gctgcggcgc tctgcaagat ggccgaccgc ggccagatca
     3301 cgggcggact gctcgacggg ccgctcgcct tcgacaacgc cgtctcaccc gacgcggcgc
     3361 gcgtgaaggg catacgctcg gccgtggccg gcgtggccga catactcgtg gtgcccgatc
     3421 tcgagagcgg caatatgctc gtgaagcagc tcgagtacat gggccatgcc gacagcgccg
     3481 gtatcgtgat gggcgcgcgc gtgccgatcg tgctcacgag ccgcgccgat tcgcgccgta
     3541 gccgcctcgc ctcctgcgcc atcgcgctga tgctggcgca ccgctttcgc gtcgcaccgc
     3601 cctgagcacc gcgcaccatg accccactga tactcgtgct gaactgcggc tcatcgagca
     3661 tcaagttcgc gctcttcgac gcgggccgcc gtccgctcgc gcgccagcca ctatggagcg
     3721 gcaagatcga gggcatcact ggaacacatg cgacctgcag ggaaaccggc cttgcgcccg
     3781 tatcggtcgc actggatgcg gcgcgaccgt acgacgccgc actggcacat atccgcgagc
     3841 gcgtccgcgc gcacatcgcc agtgaggggc gcattgcggc cgtggcgcac cgggtcgtgc
     3901 atggcggcag caggtacttc gagccggtac ggctcgacgc cgcggtattg gccgatctga
     3961 agagctatgt gccgctcgct ccactgcacc agccgtttgc gctggaggcg atcgagtcat
     4021 tgttcagtct cgcgcccggg ctgccgcagg tggcctgctt cgacaccgcc ttccatcgta
     4081 cgcaggagcc ggtcgagcag atgttcgcgt tgccgtggtc ggcctgggag cgcggcgtgc
     4141 gccgctacgg cttccatggc ctgtcgtacg agtacatgac tcacgcgctc gccgagcgct
     4201 acggcgatgc cgcgcggggc cgcacgctgg tcgcacacct cggcagcggc gccagcctct
     4261 gcgcgatgag caatctcgcg agcgtcgcca ccagcatggg cttctcggcg ctcgatggcc
     4321 tgatgatggg cacgcgctgc ggcacgctcg atccgggcgc ggtgatttac ctgatgcaga
     4381 tcgagaaact cacgctcgac gaagtggcga gcatgctcta ctacgactcc ggcctgctcg
     4441 gcgtctccgg cgtgtcggcc gatccgcgtg aactgctgcc gctcgaggcg gacaacgagc
     4501 gcgtgcgcat cgcgctggcg ctctacgtgc gccgtctcgt gcgtgaaatc ggcgcgctga
     4561 ccgcgctgct cggcggcctc gacctgctgg tgttcaccgc cggcatcggc gaacacaacg
     4621 ccgtactgcg cgcacgggtc tgcgagcaac tgggctatct cggcgtgcgc ctggacgccg
     4681 cagccaacgc gcgccacgcc gccgtcatct cggaccgcga cagcgcggtg acggtgggta
     4741 tcgaacccac caacgaggaa tggatcgccg cctcgcacgc ggcgcgactc ccggggctcg
     4801 taccgtgaac gatcgctcgg caggctgtca gttcccgcca ggcttccagt acggcgcgat
     4861 ccggtccgcc aggaccaggc tggcgcgctg cgcgggcacc acgcggcgcg ccgtcgatgc
     4921 gcgtgatcag gtcatcgacc tgcaacccgc gcacacaaca ccggggtgtt gcatgatgca
     4981 cctgcaatga gtgcgcgccc cgtattgcag cgcctgctgc tgtcgctcgg cctgggtttc
     5041 ctgctcctcg cttcggcgga aggggtggcg caacaggcag cacccggtgc acttgaagtg
     5101 ttcgtgcgcg atggctgtcc gcactgcgat gccgcgaagt ctttcctcgt ggatttcgcg
     5161 gcagagcgcc ccgacctgcg catcgtgttg cgacccgtcg aggatccgcg cacgagggac
     5221 gaactggtcg agcacttccg gcgggccggt gcgtggccgc ccgccgtgcc caccttcgtg
     5281 tacgaagggc gcatgctcgt gggcttcgac aatccggaaa tcacgggtgc tgcagtccgt
     5341 gcgctggtgg ggccgctgga attgccgccg cgcgacgaag tgcccacgat cctcggcgga
     5401 ctcagcgctg cgaagctcgg cctgccgctt ttcacgctat cactcggact gctcgacggc
     5461 ttcaatccgt gcgcgatgtg ggtgctgctg ttcctgcttt cgctgctggt ccgcctgcgg
     5521 gatcgcaggc gcatggccat catcgccgga accttcgtgc tcgtgagcgg cgcggtctac
     5581 tacgccttca tggccgcgtg gctcaacctg ttcctggcaa tcggcatgtc gaacgccgtg
     5641 cgatggacac tcgccggcat tgcgctgctg atcggcgtcg tcaacgtcaa ggacttcgtc
     5701 gcgccgggca ggggcgtgtc gctctccata cccgaagcgg cgaagccggg cctgtacgcg
     5761 cgcgcccggt cggtgctgca ggtcgagacg ctcactgcat ccctcatcgg cgtcgcgatg
     5821 ctcgccgtgt tggtcaattt cgtcgaactg ctgtgcaccg ccgggctgcc ggcgatcttc
     5881 accgcggttc ttgccgagcg cgatctctcg acgggcggct actacgccta tctcgggctg
     5941 tacatcgccg ggtacatcgc ggatgacgcg ctcatggtga ccctggcggt gctcgccctc
     6001 ggcaatcgca gactgacgca gcgcaccggc cgctggctga agctcctgag cggcgtcttg
     6061 atgctggcgc tcggcgcgat cctgtttttg cgaccgcaat ggctgtcata ggcgagctgc
     6121 caatgccttg aaccactcca ttcaccggcg cggcgcaggc ccgctggcgg acggcgggcg
     6181 gccaaggcaa tcgtggacta caatcactgg catgaaccgc ttacatggcg caaccaggcg
     6241 cacagccctc ctcgccctcc tcgcgatcac ctgcggcgca ctgtccgtcg cctgcacgaa
     6301 tcacgaagac gcgagtcccc cggtcgtcga cgatgccacc gcggaccacg ccgcgcacat
     6361 ggaccacgcc gcacaggcca cgagtggggc ggcgatcggc cagcttgcgc tcgacgcggg
     6421 ccgcaagtgg accaccgacg cgccgctgcg cgcagggatg gcggcgatcc acgaggcatt
     6481 cgacgcggaa catgcggcga tcgatgccgg cagcatggag gacgcccggt acgaggcgct
     6541 cgctgcgcgc atcgacactc atgtgcagga catcgtgcgc aactgcaagt tgccgccggc
     6601 ggccgacgcc aacctgcact acatcatcgc ggacctggcg cgtggcgcgc aggagatgcg
     6661 cggccgcgat ccggcccgta cgcgccgcga tggcgccgtg ctcgtgcacg gcgcgctggg
     6721 cgcctacggc gagttcttcg acgatccgga gttcggcgcc gccggcgagc tgccgtagcc
     6781 gctgcccgac acacgcgcgg gtgcatccgc actcgtggcg ccctgcgtca atccgtcgca
     6841 cgtctccatg gcctcgtacg ggcgagaatc cgcattccgt ctgttcatcg aggtagagaa
     6901 ggatgcgtga ctttcccgcg tcaatcgccg gcctgttgct ggtgggtggg ttcatgggcg
     6961 caacgcaggc atgggccggg gacaccgagc aggaaggatc cacgaaggag agcgccctgg
     7021 tcaccgtcgt cgtgaccgcg gtggcgccgc agtcaccgct cacgttcgtc accgacccga
     7081 agttgccgcg gcagcccgtg ccggcaagtg acggcgccga ctatctgaag acgattgcgg
     7141 gtttcagcgc catacgcaac ggcggcacca atggcgatcc cgttctgcgg ggcatgttcg
     7201 gctcccgcct gaatattctc accgacgacg gttcgatgcc gggcgcgtgc ccctcacgca
     7261 tggacaatcc cctatcgtat gtgtcgccgg agacctatga ccggttggtc gtcatcaagg
     7321 gaccgcagac ggtcctgtgg ggcccgggcg catccgcggg cacgatccgt ttcgagcggg
     7381 atgccgacgc ggaccgattc gaagacgccg gactgcgcgt caacgccaac gccctgggcg
     7441 gcaccttcgg gcgcaacgac caggtactcg acgtcatcgg cggtgcgggc gtcggctatg
     7501 cgcggctgag tgccaatcgc tccgaagcgg acgactacga ggacggcgcc gggcgcaccg
     7561 ttccgtcgag ctgggaaaag tggaacgccg acgcagcgct cggctggacg ccgtcggccg
     7621 gcaccctgct cgagttctcg gccggaacgg gtgacggcga ggcccgctat gccggtcgca
     7681 gcatggacgg gtcgcagttc gaacgcacca gttacggcct gcggttcgag aagacggtac
     7741 tcggcggtgc gctgaaagac gtgaaggcga acgtgttcta caaccacgcc gatcacgtca
     7801 tggacaacta cacgttgcgg gaaccgaatc cgatgagttc gatgccgggc ccgtttgcct
     7861 cgaacgtgga tcgacgcacg acgggaggcc gcgtggcggc cagttggcga ctgtcggcca
     7921 tcgacatcac cgccggtgcg gatacgcagc gcagccgcca tcgcaagcgc aatgccatgg
     7981 ggcgcgacgc ctatgcggac cagccccggc agaccgatgc cgagatcgcc ggcagcggca
     8041 tattcgccga actcacatgg cgattcggcg aggacagccg cgccgtgttc ggcggccgcg
     8101 gcgatcgtgc cgaagtcacc gaccggcgtc agacgagcgg cggcatgatg ccgatgccga
     8161 atcccacggc cgacgtcacc cgccgcgaaa cactcgccag cggtttcgcc cgctatgagc
     8221 acggtgcacg cgaccggccc gtctcatggt tcgccggcat tggccatacc gcccgcatgc
     8281 cggactactg ggaactgttc tcgccgggca gcggtccgat gggaacggta aacgcgttcg
     8341 ctgcccttgc cccggaggag acgacgcagc tcgacctcgg cctgcagtat cgaaccgcga
     8401 gagtggacgc gtggatgtcc gcatacgccg gccggatcga tgatttcatc ctgttcacct
     8461 acggcagcgg cggcatgatg ggactcatga cccgtgtgaa ccaggtcgac gccgctgtgc
     8521 gtggcgcgga agcaggcatc gagctgcggc cgctgccgca atggaaggcg gctgcgacgc
     8581 tcgcctatgc ctgggcggag aatcgtggca cgggggcagc gctaccgcag attccgccgc
     8641 tcgatgtccg gatctccgtc gcttacgaca acgcccgctt ttcggtgggg gcgctgctgc
     8701 gcgcggtcga cggccagggt cgtgtcgcgc cgggacaggg caatgtcgcc gggcgtgacc
     8761 tgggccggag cgccggattc gccgtgctgt cgttgaacgg cgcgtaccgg ctttcacctc
     8821 gcctgagcct cgctgccggc atcgacaacc tgctcgaccg cgactatgcc gagcacctga
     8881 atctcgccgg cagcgcggat ttcggctatc cggccgaacc ggtgcgcatc cacgagccgg
     8941 gtcgctcggc gtggctgaaa gtgacctgga acaccggcgg agggtgaact tcgggcgggg
     9001 cgccgctaaa gtcagtgcgt ggaacacccc gcctcactct ccttcgcccg gctgctggtc
     9061 aacctgcgct ggctgtcggt ggcaggccag gcgctgacca ttctggtcgt cacccgaccg
     9121 ttgggcgtgg cgctgcccga aaccccgctg tgggcgggag ttggcgcact cgccctgttc
     9181 aacctgttcg ccgcccggcg agtgcgcacg gcgggcgacg tgagcgtccc ggaagtgttc
     9241 gcgcacatcc tggtcgatat tgtcgcgctc acgtggatgg tggcgtggag cggcggcatc
     9301 gaaaatccgt tctcctccct tttcctgcta cccatcgccc tgtcgatcct ggcgctgccg
     9361 gggcgctggg tctgggcgac ggctctcgct gccatggccg ggtatgtcgc gtcggcattg
     9421 ctcgcacgcg agttgccgca tgtgcatggc gtgctcggcg ataccttcag catccacaag
     9481 gcaggcatgc tcgcgaactt cgcgctgtcg gccgtggtga tcctggtgtt cttcacccgc
     9541 atggcggcgg catggcgccg cagcgaacag gaagtggccc gcctgcgcga gcagttcgcg
     9601 cgcaacgaag ggatcattgc gctcgccacc cacgccgcgt cggtggcaca tgagctgaac
     9661 acgccgctag gcaccttgac cctgatggtg gaggacctgg cggccgaagc cggcagcgat
     9721 gcgcatcgcg aagactacgc cacgatgcag tcgctgctgg cggtatgccg cgaacgggtg
     9781 cgcgacctgg cggcgccggc cgatgccggt caagcgggca atcgccggga gagcgtcgat
     9841 ctcgaacgcg tgatcgagcg ctggcaactg gtgcggccga cgatcgatct gcgcaggtcc
     9901 ggcgtcacga gcgggctcgg ctcggtcgat cccgccgttg gccatctgct gctggcgctc
     9961 ctcaacaatg ccgcagacgc cggcgaacag gtcggcgcgg cgcgcgtcga cctgcacatc
    10021 gacagcggcg ggcaagggct gcgggtgtcg attcgtgact acggggtggg tttcgagcac
    10081 gcccagccgg tactgccggc gaaactgttc cggacgaaca agcccggcgg catgggcatc
    10141 ggtctcgcgc tgtcgcacgc gactgtcgag cgactgggag gctcgctgtc catgctagcg
    10201 gccaccgacg gaccgggcgt gcaggtgtcg ttcgagttgc cggcgctgag cacggcatga
    10261 cgggcaaggc gagcgtagcg cagggcccgg gcctgctggt cgacgacgat gagctgtatc
    10321 tgcgtaccct gcagcgatcg ctgcagaggc gcggctacgc gaccgtgacg gcgaccagcg
    10381 gcgacgacgc gctcgccgcc gcgaaggcgc atcgccccgc gtatgcactc gtcgatctca
    10441 aactgggcag catgtccggc ctcgcactca tcgctccctt gcgggcggtg cgtgacgaca
    10501 tgcggatcct gctggtgacg ggctatgcga gcattgcgac ggccgttgaa gcgatcaagc
    10561 gtggcgccga caattacctg atgaagcccg tcaccgcgga tgcgatcgtg cgcgcgttga
    10621 acgaggagac ggagagcgtg cctgtgccgg agggcacgat ggtgccgctg gcacgcgtcg
    10681 agtgggagca tatacagcag gcgctgggcg aaacccgggg aaacatctcc gccgctgcgc
    10741 gcctgctcgg catgcatcgc cgctcgttgc agcgcaagct cgccaagcgg ccgggaccgg
    10801 aacgcagggg cgaatgatga gtccgggcgg agcaccagtc atatcagttg cgtctaatat
    10861 aagaacttgc taatttagcc ttaggttggc ggcgagttcg cgtccaccgg cccgttcaga
    10921 accttggagg gcacaccatg acacgcatcg tcgtattggg cgccggcatc ggcggcatga
    10981 gcatcgccta tgaactgcgc gggactctgg gcaagggccc ggaaatcacc gtgatcggcc
    11041 agggcgaaca cttttcgttc acgccttcga atccatgggt cgccgtgggg tggcgcgagc
    11101 cggccgacgt cgagatcgcg gtggcaggtt acctcgcgaa gcagcacatc ggcttcgaat
    11161 ctgcaggagc cgagcgggtc gagcctgcag acaatcgcgt gctgctgcgc gatggccgcc
    11221 acatcgagta cgactacctg gtgatcgcca ccggtccgcg cctcgccttc gacgaagtca
    11281 cgggactcgg tcccgacggc ggc
//
LOCUS       DAOQVY010000073        11225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_6524_length_11225_cov_5.181289, whole genome shotgun sequence.
ACCESSION   DAOQVY010000073 DAOQVY010000000
VERSION     DAOQVY010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11225
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6524_length_11225_cov_5.181289"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="PKN91_07955"
     CDS             complement(<1..1253)
                     /locus_tag="PKN91_07955"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNS28044.1"
                     /translation="MTGSDRTAWRVHKFGGSSVADAACFERVAKIIEASPDERLGIVL
                     SACRGVTDALYQLVQIAERQQDGVRRELDQLRARHAGIATATLGETGARLWLAAFDRD
                     CHDLEGILNTVRLTRSASGAVRDLIVGYGELWSTRLFREYLAHRGQRKGAIDWVDART
                     VLIVEWGPLGPAVQWGESRARLAELAGGPGPRTLVITGFIASDRNGVQTTLGRNGSDF
                     SASIFGALLDAGEIVIWTDVDGVLSADPRRVPDARVIDSLSYSEAMELAYFGAKVIHP
                     QTMAPAVGQHIPIRIRNTFAPDRPGTLICAEPRSSMPVKGITSIEQVALVNVEGAGMI
                     GVPGTAHRLFGALREADISVILISQGSSEHSICCAVPEAQADAAMRAVREAFARELAD
                     GQIQSVDIDRDLAILAAVGDGMAGMP"
     gene            1333..2304
                     /locus_tag="PKN91_07960"
     CDS             1333..2304
                     /locus_tag="PKN91_07960"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNS28045.1"
                     /translation="MEHELHRLLPFYASSRAAAEPLALALVLRTAGSTYRKPGAWMLI
                     ARDGRCAGLLSGGCLEGDLREHAVRVIESGVAVIVSYDMRGPDDELWGLGTGCEGAMD
                     ILLMRVSEVEHWQPLDALARAQQRGTACEFGVVISATPGGLPLGAIELSPVGSTDVFI
                     VRIDPPPHLLLLGGGPDALPVAELASFLGWRVTVVDHRPAYTQPERFPAGTRVITARA
                     GEFAAQCDVAGFDAAVVMSHHLDSDRAWLLELAATSIGYVGLLGPPPRRARLLRELGA
                     SAAPLQGRLRAPVGLDLGARSPTTIALAIVAEIQAHLAGTSAGPLSH"
     gene            2304..3116
                     /locus_tag="PKN91_07965"
     CDS             2304..3116
                     /locus_tag="PKN91_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNS28046.1"
                     /translation="MEALPAFALALAGTGLVAGLIAGLLGVGGGIVIVPVLDAVLIGA
                     GVAPEWSMNVAVATSLATIVPTSIASARAHHARGAVNWPLARRWGGPIVAGAFVGGLL
                     AARASSAGLAALFGIVAAVVATRMFLPFDHLRLAPSPPRGLPGAATGTGIGALSAMLG
                     IGGGTLAVPALTLAGEPIHRAVATAAFFGLLVSGPGTLAYLLALPAAELPPGTVGLVN
                     LPGVALIALATIVTVPLGARIAHALGPRALSMAFGAFLLMVAARMLWRVIVV"
     gene            3236..4420
                     /locus_tag="PKN91_07970"
     CDS             3236..4420
                     /locus_tag="PKN91_07970"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNS28047.1"
                     /translation="MYINFWYPVIRTVDLGTTPQRIRILAHNFVVFRDAAGKPAVLSD
                     TCVHRGGSLSGGKCKEDGTVQCPYHGWRFDRDGKCTRIPSLGINAKIPPRARVDAYPV
                     EERYGIVFAFLGDLPAAERPPIMPIFEADDPAWRSTVVMFEVPFHYERSIENGLDPAH
                     NEYVHSTHGFQGERESEYRVNELVPEQNNPWGFGFMHTYDAPPLRKSVMRLARTKGGK
                     MQAGSGTYGPNQMWTYIHMTETKAMHQYLFEAPIDETHTRVFLVNNRNIVFYRSGLRA
                     KLNSWFDKKVTERNMFVAGEDIVVVREVQPRLTPPSTARELMMPADKVIAQYRDKLEE
                     FEKKGWRLDMQRIKRDYERGDVIYAIPSPGRRESKGWVLEAVPLIPASPAPGQMAQRA
                     TG"
     gene            4470..5000
                     /locus_tag="PKN91_07975"
     CDS             4470..5000
                     /locus_tag="PKN91_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28048.1"
                     /translation="MDELAILRGQIATERRHLIAVKGACRAALPAARQTTGADEFARI
                     AGDYLQHIGRRMLAQDHAHVVLLRPRLAAGDVASHRLLDELEATLGQLRTALQALETG
                     TDRIAGLFDYLDFIDSVMAKRRHALEPLLAAHYSIEDWRAAAFVDADSILEERERYAA
                     CRAAAPAGAFAPEGET"
     gene            5003..5632
                     /locus_tag="PKN91_07980"
     CDS             5003..5632
                     /locus_tag="PKN91_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNS28049.1"
                     /translation="MDMPGGELPSLHAIVLAAGASTRFGSPKQLVRIDGRPLLHAVVT
                     RAVEVAGHSVTVVLGAHAGELTALLRHTPASIVVNRDWEEGIASSIRAGMAQLPGAAD
                     GVLLMLADQASVTADDLRRLAGAWRRQPDSILAAQYSGVTGVPAIFPRWAFRDLAQLR
                     GDRGAQLLLQRYADRVARLRMVSAAIDIDRPEDLLAVETGIDRRPPPPQ"
     gene            5663..6985
                     /locus_tag="PKN91_07985"
     CDS             5663..6985
                     /locus_tag="PKN91_07985"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNS28050.1"
                     /translation="MQPLTGIPYAAVAVGLLVLALVAWLANWVTRRVLLAVVGRAARA
                     STSKWDDAIMGRRVLARLANVVPALIVLGGIGMVPGVPDWLDLVMRNVALAYVALSVA
                     MAIGNLLNALNDLYEQTSRHATRRPIKGYLQLVKIVVYIIAGVVIIASLIGRSPLVLL
                     TGLGAMTAVLMLVFKDTLLSLVASMQLSSNDMLRVGDWIEMPSQNADGDVIDISLHTV
                     KVQNWDKTISTIPTWKLISESYRNWRGMSESGGRRIKRALLIDQSSVRFLEPEERRHL
                     RRIALIDEYLDKQQAELDAYNAGLAAAGKDPVNNRRATNLGTFRAYITAYLRSHPRVA
                     QNMTLIVRQLNPTPTGLPLEVYCFSADVRWAEYENLAGDIFDHLLAVLPEFGLRLYQA
                     PGGADVAAALARLGGGADSSPQATLTPQVHPPATAAARHPGHAAPPAR"
     gene            complement(6912..8813)
                     /locus_tag="PKN91_07990"
     CDS             complement(6912..8813)
                     /locus_tag="PKN91_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNS28051.1"
                     /translation="MTSRKTRPLRTLLIANRGEIACRIVRTARRLGMRTVAIYSDADV
                     DALHVRTADVAVRVGPAPARASYLDIAAVIAAARQAGADAIHPGYGFLSQNAEFVEAC
                     ADAGIVFVGPGAAAMRAMGRKDEAKALMQAAGVPVVPGWQGKDQTDATLAREAARVGF
                     PLLVKAIAGGGGKGMRVVRTAAELPAALSAARGEAARSFGDDRVLLERYVESPRHVEV
                     QVIADTHGGCIHLFERDCSLQRRYQKVIEECPAPGLTPDLRARLHAAAVKAAQAVGYV
                     NAGTVEFIVAGEDGWFLEMNTRLQVEHPVTEAVLGIDLVEWQLRIAAGEALPELTREP
                     RGHAFEARLYAEDPRHGYLPAGGRIEVLRWPDGDVRIDAAVEEGDRVVTDYDALLAKL
                     IVHGESREAAFGRLEAALLATRVEGVTTNLAALVALARNPEFRAGLMTTGLIDGRGES
                     LLPDATAQRERAARMAAAALLAPTSETTQDPWAARDGWRVQGERAVSIRLADISVPGA
                     NSTLIATAGSEPAGSDSIDLSGEEIGVWLDGEHFRFARVARGAPAESGPTGGEVLAPM
                     PGTVLDVLVTAGKVVARGDPLVVLEAMKMEHTLRAGGAGRIVEVNVQAGQRVRDGERL
                     LSLADAPAA"
     gene            complement(8806..9963)
                     /locus_tag="PKN91_07995"
     CDS             complement(8806..9963)
                     /locus_tag="PKN91_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HNS28052.1"
                     /translation="MADFDFDLGESAELLRETVRHFARERIAPRAQQIDRDNRFPRDL
                     WPEFGELGLHGVTVSIEDGGSGLGYLDHVIAMEEVSRASASVGLSYGAHSNLCVNQLR
                     RWGSTEQKRRYLPKLLSGEHVGALAMSEPESGSDVVSMRLTATRAGDHYVLDGRKMWI
                     TNGPEADVVVVYARLPGTRGTSGITAFIVERGMPGFHAAQKLDKLGMRGSDTSELVFE
                     GCRVPLANLLHEEGRGVEVLMSGLDYERVVLAGGPLGIMQACLDTVLPYVRERKQFGQ
                     PIGQFQMMQGKIADLYTTLNACRAYVYSVARACDQGRTTRYDAAGCILYASERATQCA
                     LETIQALGGNGYINDYPAGRLLRDAKLYEIGAGTSEIRRMLIGRELFAADD"
     gene            complement(10083..10727)
                     /locus_tag="PKN91_08000"
     CDS             complement(10083..10727)
                     /locus_tag="PKN91_08000"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNS28053.1"
                     /translation="MMLRPGPDRASRGSATAEFLLIAIVVLLPLVFGTLDLALLAVTR
                     HTLGVASLMAARAGAVEHGDRGVMQRTLARGLSPLYAVHGDSEALARSYADVLRPDRT
                     RIEIWSPVAASFADFGEMVDGRVEIPNVWPQEQVRVGAASRQTIVEANQLGLRVSICR
                     TLLFPLTASMITPVLGARDPDTFGQGCRAQRGVALHARVLVHMHSPARRDAMGI"
     gene            complement(10705..11058)
                     /locus_tag="PKN91_08005"
     CDS             complement(10705..11058)
                     /locus_tag="PKN91_08005"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNS28054.1"
                     /translation="MRERRLLHNGRLLAARVFACETFLERARGVTLRRPMQAHAIVKI
                     APCAGVHTVGLHDPIDVVFTNREGRVLRCVHELQPWRAAVCGRAFVAWEMPRGMCRRL
                     SIRPGDRLDDAPPRP"
BASE COUNT         1750 a         3916 c         3776 g         1783 t
ORIGIN      
        1 ggcatgccgg ccatgccatc gccgaccgcc gcgaggatcg cgaggtcgcg atcgatgtcg
       61 acgctctgga tctgtccgtc ggcgagttcg cgcgcgaagg cctcgcgcac ggcccgcatg
      121 gccgcgtcgg cctgcgcctc ggggaccgcg cagcagatcg aatgctccga actgccctgc
      181 gagatcagga tcacggagat gtcggcctcg cgcaatgcgc cgaacagccg atgcgccgtg
      241 ccggggacgc cgatcatgcc cgcaccctcg acattcacca gcgccacctg ctcgatgctc
      301 gtgatgccct tgaccggcat gctggaacgc ggctccgcgc agatcagcgt cccgggccgg
      361 tccggcgcga aggtattgcg gatccggatc ggtatgtgct ggccgacggc cggcgccatg
      421 gtctgcgggt ggatgacctt ggcgccgaag tacgcaagtt ccatcgcctc gctgtaggac
      481 agcgagtcga tcacgcgcgc atcgggcacg cggcgcgggt cggccgacag tacgccgtcg
      541 acatcggtcc agatgacgat ctcgcccgca tcgagcagcg cgccgaagat cgaggcggag
      601 aagtcgctcc cgttgcggcc gagggtcgtc tgcacgccgt tgcggtccga ggcgatgaag
      661 ccagtgatca cgagcgtgcg cggacccggg cccccggcca gttcggcgag gcgcgcacgc
      721 gactcgcccc actgtacggc cggccccagg gggccccatt cgacgatcag taccgtgcgg
      781 gcatcgaccc aatcgatcgc gcccttgcgc tgaccgcggt gcgccaggta ttcgcgaaac
      841 agccgcgtcg accacagctc accgtagccg acgatcaggt cgcgcaccgc gccggatgcc
      901 gaccgggtca ggcgcacggt attgagtatg ccctcgagat cgtggcaatc gcggtcgaag
      961 gcggcgagcc acaggcgggc gcccgtctcg ccgagtgtcg cggtcgcgat gccggcatgc
     1021 cgggcgcgca gctggtcgag ttcgcgccgc acgccgtcct gctgacgctc ggcgatctgc
     1081 accagctggt agagcgcatc ggtcacaccg cgacaggcgg agagcacgat gccgagtcgc
     1141 tcatcgggcg atgcctcgat gatcttcgcg acgcgttcga agcaggccgc atcggcgaca
     1201 ctcgaaccgc caaacttgtg gacacgccag gcggtccgat cggaaccggt cattgattct
     1261 ctcgagaggg gggcaaaaac ctcggaatgc taatgcctgc atgccattgg acgcaatata
     1321 atgatcggat acgtggaaca tgaactgcat cgcctcctgc cgttctatgc cagttcgcgt
     1381 gccgccgccg agccccttgc gctcgcgctg gtgctgcgca cggcgggttc gacctatcgc
     1441 aagcccgggg cgtggatgct cattgcccgg gacggccgct gcgccggcct gctctccgga
     1501 ggctgtctcg agggcgacct gcgcgaacac gccgtgcgcg tcattgaaag cggagtagcc
     1561 gtgatcgtca gctacgacat gcgcggtccc gatgatgaac tgtgggggct cggcaccggc
     1621 tgcgaaggcg caatggacat cctgctgatg cgagtcagtg aggtggaaca ctggcagccg
     1681 ctcgatgcgc tcgctcgtgc acagcagcgg ggcacagcct gcgagttcgg cgtcgtgatc
     1741 tccgctacgc ccggcgggtt gccgctcggt gcgatcgagc tttcgcccgt cggttcgacc
     1801 gatgtcttca tcgtgcggat cgatccgcct ccgcatctgc tgctgctggg cggcgggccg
     1861 gatgcgctgc ccgtcgccga actcgcgagc ttcctcggct ggcgggtgac cgtcgtcgat
     1921 caccgccccg cctacacgca accggaacgc tttcccgcag gcacgcgcgt gatcaccgcg
     1981 cgcgcagggg aattcgcggc acagtgcgac gtcgcgggct tcgatgctgc cgtggtcatg
     2041 agccaccacc tcgactccga tcgtgcatgg ctgctggaac ttgcggcgac ctcgatcggc
     2101 tatgtcgggc tgctcggtcc gccgccgcgg cgcgcacggc tgttgcgcga actgggcgcc
     2161 agtgcggcgc ccttgcaggg acgcctgcgc gcaccggtcg ggctcgacct cggtgcgcgc
     2221 tcgcccacga cgatcgcgct cgccatcgtc gccgagatcc aggcgcacct cgccggcacc
     2281 agcgccggcc cactgtcgca ctgatggagg cgctgccggc tttcgcactt gcactggctg
     2341 gcacgggact ggtcgccggc ctgatcgccg gcctgcttgg tgtcggcggc ggcatcgtca
     2401 tcgtgccggt actcgacgcc gtgttgatcg gcgcaggtgt cgcgcccgaa tggagcatga
     2461 acgtcgccgt cgccacgtcg ctcgcgacga tcgtccccac ctccatcgcg tcggctcgcg
     2521 cgcaccacgc acgtggcgcg gtcaactggc cgctcgcgcg ccgctggggc ggccctatcg
     2581 tcgccggtgc gttcgtcggc ggcctgctcg ctgcgcgcgc atcgagcgca gggctcgcag
     2641 cgcttttcgg catcgtcgcc gctgtggtgg cgaccagaat gttcctgccg ttcgaccacc
     2701 tgcggctcgc cccctcgccg ccacgcggcc tgcccggcgc agccaccggt accggcatcg
     2761 gtgcgctatc ggccatgctc ggcatcggcg gcggcacgct ggccgtgccg gcattgaccc
     2821 tggccggcga accgatccat cgcgccgtgg cgaccgccgc attcttcggg ttgctggtca
     2881 gcgggcccgg caccctcgcc tacctgctcg cattgcccgc cgccgaactc ccgcccggca
     2941 ccgtcggcct cgtgaatctc cctggtgtcg cgctgattgc gctggccacg atcgtcacgg
     3001 tgccgctcgg cgcacgcatc gcgcacgcac tgggcccgcg agcgctctcg atggctttcg
     3061 gggcctttct gctgatggtc gccgcgcgca tgctgtggcg ggttattgtc gtctgactgc
     3121 ccgccgcccg acaggcggac gcgcaccagg cgctgacaat tctttacagc ggcgcgcata
     3181 tcgtcccgca ttgtcttttg ctaacgtttc cgtaacggga tttcgcgaga cgaccatgta
     3241 catcaacttc tggtatccgg tcatccgtac cgtcgatctc ggtacgactc cgcagcggat
     3301 tcgtatcctc gcccacaatt tcgtcgtgtt tcgcgacgcg gccggcaagc ccgcggtgct
     3361 ttccgacacc tgcgtacacc gcggcggctc gctgtcgggc ggcaagtgca aggaggacgg
     3421 caccgtgcag tgcccgtacc acggctggcg cttcgaccgt gacggcaagt gcacccgcat
     3481 tccctcgctc ggcatcaatg cgaagattcc accgcgcgcg cgcgtcgacg cctacccggt
     3541 cgaggagcgc tacggcatcg tattcgcctt cctcggcgac ctgcccgctg ccgagcggcc
     3601 gccgatcatg ccgatcttcg aggccgacga ccctgcctgg cgttcgaccg tggtcatgtt
     3661 cgaagtcccg tttcactacg aacggtcgat cgagaacggg ctcgatcccg cgcacaacga
     3721 atacgtgcac tcgacacacg gcttccaggg tgagcgcgaa agcgagtacc gcgtcaacga
     3781 actcgtgccc gagcagaaca acccctgggg tttcggcttc atgcacacct acgatgcgcc
     3841 gccactgcgc aagtcggtga tgcgtctggc acgcaccaag ggcgggaaga tgcaggccgg
     3901 ctcgggcacc tacggcccga accagatgtg gacctacatc cacatgaccg aaacgaaggc
     3961 gatgcaccag tacctgttcg aggcgccgat cgacgagacg cacacacgcg tcttcctggt
     4021 caataaccgc aacatcgtgt tttaccgcag tggcctgcgc gcgaagctca acagctggtt
     4081 cgacaagaag gtcaccgaac gcaatatgtt cgtcgccggc gaggacatcg tggtcgtgcg
     4141 cgaagtgcag ccgaggctga caccaccgtc gacggcacgg gagctgatga tgcccgccga
     4201 caaggtgatc gcgcagtatc gcgacaagct tgaggaattc gagaaaaagg gctggcgcct
     4261 cgacatgcag cgaatcaagc gggactacga gcgcggcgac gtgatctacg cgattccgtc
     4321 accggggcgg cgcgaatcga agggctgggt gctcgaagcg gtgccgctga taccggcatc
     4381 gcccgcaccg ggacagatgg cccaacgcgc gaccggctga ccggcaaccg acccgcccgg
     4441 cgggccgcgt gggctgctac agtccacgta tggatgaact cgcgatactg cgcggccaga
     4501 ttgcgaccga gcgccgccac ctcattgccg tgaagggcgc atgccgcgcc gccctgccag
     4561 ccgcgcgcca gacgactggt gccgacgagt tcgcccggat cgccggcgac tacctgcagc
     4621 atatcgggcg ccgcatgctc gcgcaggacc atgcacatgt cgtgctgctg cggccacggc
     4681 tcgctgccgg cgatgtggcg agccaccggt tgctcgacga actcgaggcg acactcggac
     4741 agctgcgcac cgcactgcag gcgctcgaaa ccgggaccga tcgcatcgcg ggcctgttcg
     4801 attacctgga tttcatcgat agcgtcatgg cgaagcggcg ccacgccctc gagccgctgc
     4861 tggccgcgca ctactcgatc gaggactggc gcgcggccgc gttcgtcgac gccgacagca
     4921 tcctcgagga gcgcgagcgc tatgcggcct gccgggcggc cgcgccggcc ggtgcgttcg
     4981 cgcccgaggg ggagacctga acatggacat gcccggcggc gagttgccca gcctgcacgc
     5041 catcgtgctc gccgcggggg cttcgacgcg gttcggttca ccgaagcagc tcgtgcgcat
     5101 cgacggtcgg ccactgttgc acgcggtggt tacacgtgcg gtcgaggtgg cggggcattc
     5161 ggtcactgtc gtgctgggcg ctcacgccgg cgagctcacg gccctgctgc gtcatacgcc
     5221 tgcatcgatc gtggtcaacc gcgattggga ggaaggcatc gccagttcga tccgcgccgg
     5281 gatggcgcag ttgccgggcg cggccgacgg cgtgctgctg atgctcgcgg accaggcaag
     5341 cgtcaccgcc gacgaccttc gccgtctcgc cggcgcgtgg cggcgccagc cggattcgat
     5401 cctcgcggcg caatacagcg gtgtcaccgg cgtgccggcc atctttccgc gctgggcttt
     5461 ccgcgacctc gcgcagctgc gcggcgatcg cggcgcgcag ttgctgctgc agcgctacgc
     5521 cgatcgcgtg gcgcgcctgc gcatggtctc ggccgccatc gacatcgatc gaccggagga
     5581 tctgctggcc gttgaaacgg gcatcgaccg acgcccgccg ccgccgcagt agcacatgca
     5641 ggagttcatc gactccaacc tggtgcagcc gctgaccggc atcccttacg ctgccgtggc
     5701 cgtcggtctg ctggtgcttg cgctcgtcgc ctggctggcc aactgggtga cgcgacgcgt
     5761 gctgctcgcc gtggtcgggc gcgcggccag ggcgtcgacg agcaagtggg acgacgcgat
     5821 catggggcgt cgtgtgctgg cgcgcctcgc caacgtggtg ccggcattga ttgtcctcgg
     5881 cggcatcggg atggtgccgg gcgtgccgga ctggctggat ctggtgatgc gcaacgtcgc
     5941 ccttgcctac gtcgcgctga gcgtcgcgat ggccatcggc aatctgctca atgcgctcaa
     6001 cgacctctac gagcagacca gccgccatgc gacacggcgc ccgatcaagg gctacctgca
     6061 actggtgaag atcgtagtct acatcattgc cggcgtggtg atcatcgcct cgttgatcgg
     6121 caggtcgccg ctggtgctgc tgaccggcct cggcgcgatg acggcggtgc tgatgctcgt
     6181 cttcaaggac accctgctgt cgctggtggc cagcatgcag ctgtcgagca acgacatgct
     6241 gcgcgtgggc gactggatcg agatgccgag ccagaatgcg gacggcgacg tgatcgacat
     6301 cagcctgcac acggtgaagg tgcagaactg ggacaagacg atcagcacca tccctacctg
     6361 gaagctgatc agcgagagct atcgcaactg gcgcggcatg agtgaatccg gcgggcggcg
     6421 catcaagcgc gcgctgctga tcgaccagag ctcggtgcgc ttcctcgaac ccgaggagcg
     6481 ccggcacctg cgccggatcg cgctgataga cgagtacctg gacaagcaac aggcggaact
     6541 cgacgcctac aatgccgggc tggccgcggc cggcaaggat ccggtgaaca accggcgcgc
     6601 gaccaacctc ggtacgttcc gcgcctacat caccgcttat ctgcgatcgc atccgcgcgt
     6661 ggcacagaac atgacgctga tcgtgcgcca gctcaatccc acgccgaccg gcctgccgct
     6721 ggaggtgtac tgcttctcgg ctgatgtgcg ctgggcggag tacgaaaacc tcgccggcga
     6781 catcttcgac catctgcttg ccgtgttgcc tgaattcggc ttgcgcctct accaggctcc
     6841 cggcggtgcc gacgtggccg cggcgctagc ccggctgggc ggcggcgcgg actcgtcgcc
     6901 ccaggcgacg ctcacgccgc aggtgcatcc gccagcgaca gcagccgctc gccatcccgg
     6961 acacgctgcc ccgcctgcac gttgacttcg acgatacgcc cggcgccacc ggcgcgcagc
     7021 gtgtgctcca ttttcatcgc ctcgagcacg accagcgggt cgccgcgtgc aaccaccttc
     7081 cctgcggtca ccagcacatc gagcacggtg cccggcatcg gcgcgagcac ctcgccgccg
     7141 gtcggcccgg actccgctgg cgcaccgcgc gcgaccctcg caaagcggaa atgctcgccg
     7201 tcgagccaga cgccgatctc ttcgcccgac agatcgatcg aatcggaccc agccggctcg
     7261 ctgccggctg tcgcaatgag cgtcgaattc gcacccggca cggagatgtc ggccaggcgt
     7321 atgctgacag cacgctcgcc ctgcacgcgc cagccgtcgc gcgcggccca gggatcctgc
     7381 gtcgtctcgc tcgtcggcgc gagcagcgcc gcggcggcca tgcgcgcggc gcgttcgcgc
     7441 tgcgccgtgg catcgggcag gaggctctcg ccgcgcccgt cgatcagacc agtcgtcatg
     7501 agtccggcgc gaaactcggg attgcgcgcc agtgcaacga gcgccgcgag attggtcgtg
     7561 acgccttcga cgcgcgtggc gagcagcgcg gcctcgagcc ggccgaacgc ggcctcgcgc
     7621 gactcgccat gcacgatcag cttggcgagc agcgcgtcgt aatcggtgac cacgcggtcg
     7681 ccctcctcga ccgctgcgtc gatgcgcaca tcgccgtcgg gccagcgcag cacctcgata
     7741 cgcccgcccg caggcaggta gccgtggcgc gggtcctcgg cgtagagccg tgcctcgaag
     7801 gcatgtccgc gcggttcgcg cgtcaattcg ggcagggctt cgccggccgc gatgcgcaac
     7861 tgccactcca cgagatcgat gccgagcacg gcttcggtga ccggatgctc gacctgcagg
     7921 cgcgtgttca tctcgaggaa ccagccgtcc tcgcccgcca cgatgaactc gaccgtgccc
     7981 gcgttcacgt atcccaccgc ctgcgcagcc ttgaccgcgg cggcgtgcag acgcgcccgc
     8041 aggtccggcg tcagcccggg tgcagggcac tcttcgatga ccttctggta gcggcgctgc
     8101 agcgagcagt cgcgctcgaa caagtggatg catccaccgt gcgtgtcggc gatcacctgc
     8161 acttccacgt gccgtggcga ctcgacatag cgttcgagca gcacccggtc gtcgccgaac
     8221 gagcgtgccg cctcgccgcg cgcggcggac agcgccgcgg gcagctccgc ggcagtgcgc
     8281 accacacgca tgcccttgcc gccaccgccg gcgatcgcct tgacgagcag cgggaagccg
     8341 acgcgtgcag cctcgcgcgc gagcgtcgca tcggtctggt ccttgccctg ccagcccggc
     8401 accaccggca cgcccgcggc ctgcatcagc gccttggctt cgtccttgcg tcccatggcg
     8461 cgcatggccg ccgcgccggg accgacgaac acgatgccgg cgtcggcaca ggcctcgacg
     8521 aactccgcgt tctgcgacag gaagccgtaa cccggatgga tggcatcggc gcctgcctgt
     8581 cgggccgcgg cgatcacggc ggcgatatcg aggtagctcg cgcgcgcggg tgcgggcccg
     8641 acgcgcacgg cgacatcggc cgtgcgcaca tgaagcgcgt cgacgtcggc gtccgagtag
     8701 atcgccacgg tacgcatgcc gagccggcgc gccgtgcgca cgatgcgaca ggcgatttcg
     8761 ccgcgattgg cgatcaggag cgtgcgcagc ggccgcgtct tgcggctagt catcggcggc
     8821 aaacagttcg cggccaatca gcatgcggcg gatctcgctc gtcccggcgc cgatttcgta
     8881 gagcttggca tcgcgcagca ggcggccagc cggatagtcg ttgatgtagc cgttgccgcc
     8941 gagggcctgg atcgtctcga gcgcgcattg cgtcgcgcgc tcgctcgcat agaggatgca
     9001 gccggcggcg tcgtagcgcg tggtgcggcc ctggtcgcag gcgcgcgcga cgctgtacac
     9061 ataggcgcga caggcgttga gcgtggtgta gaggtcggcg atcttgccct gcatcatctg
     9121 gaactggccg atcggctggc cgaactgctt gcgttcgcgc acatagggca gcacggtgtc
     9181 gaggcaggcc tgcatgatgc cgagcggccc gcccgcgagt accacccgct cgtagtcgag
     9241 gccgctcatc agcacctcga cgccgcggcc ctcctcgtgc agcaggtttg cgagcggcac
     9301 ccggcagccc tcgaacacga gttcggaagt gtcggagccg cgcataccga gcttgtcgag
     9361 cttctgcgcg gcgtggaatc ccggcatgcc gcgctcgacg atgaaggccg tgataccgct
     9421 ggtgccgcgc gttccgggca gccgcgcgta gaccacgacg acatcggcct cgggaccgtt
     9481 cgtgatccac atcttgcggc catcgagcac atagtgatcg ccggcgcgtg tggcggtaag
     9541 tcgcatgctg acgacgtccg aacccgattc gggctcgctc atcgcgagcg cgccaacgtg
     9601 ctcgcccgaa agcagcttcg gcaggtaacg gcgcttctgt tcggtgctgc cccagcggcg
     9661 cagctggttc acgcagagat tggaatgcgc gccataggac aggcctacgg atgccgaagc
     9721 gcgactcacc tcttccatgg cgatgacgtg gtcgaggtaa ccgaggccgc tgccgccatc
     9781 ctcgatcgac accgtgacac catgcaggcc gagttcgccg aactccggcc acaggtcgcg
     9841 cgggaacctg ttgtcccggt cgatctgctg cgcccgcggg gcgatgcgct ccctggcaaa
     9901 gtgccgcacc gtctcgcgca gcagttcggc gctctcgccg agatcgaagt cgaagtccgc
     9961 caagatccct ctctcatccg tcccatggat ccgaaattcg gtggcgaagt gtacaccgct
    10021 gcatgcaccg tggcatgcat cgggcggcgt gccggattat tgttgtgtcg gggcgagtgc
    10081 actcagatgc ccatggcgtc acgccgcgcc ggcgagtgca tgtggacaag cacacgcgca
    10141 tgcagcgcca cgccgcgctg cgcacggcag ccctgcccga aggtgtcggg atcgcgcgcg
    10201 cccagaaccg gggtgatcat cgaggcggtc agcggaaaca gcagcgtgcg acagatgctg
    10261 acgcgcaggc caagctggtt cgcctcgacg atggtctggc gcgatgcggc gccgacgcgc
    10321 acctgctcct gcggccacac gttcgggatc tcgacccgcc cgtccaccat ttcgccgaaa
    10381 tccgcgaatg acgccgcgac cgggctccag atctcgatgc gcgtgcgatc agggcgcagc
    10441 acgtcggcgt agcttcgcgc cagtgcttcg ctgtcgccat gcacggcgta caggggcgag
    10501 agtccgcgcg ccaatgtccg ctgcatcacg ccgcggtcgc catgctcgac cgcaccggcg
    10561 cgcgccgcca tgagcgaggc cacacccagc gtgtgccggg tgacggcgag cagcgcgagg
    10621 tcgagcgtgc cgaacaccag cggcaggagc acgacgatgg cgatcagcag gaattcggcg
    10681 gttgccgaac cgcgcgaggc gcgatcaggg ccggggcgga gcatcatcga ggcggtcccc
    10741 cggccggatg gacagccgcc ggcacatgcc gcgtggcatc tcccaggcga cgaacgcccg
    10801 gccgcagacg gcggcccgcc agggttgcag ttcgtgcacg cagcgcagga cgcgcccctc
    10861 gcgattggtg aacacgacgt cgatcgggtc atgcagcccg acggtatgca cgcccgcgca
    10921 gggcgcgatc ttgacgatgg catgtgcctg catcggccgg cgcagcgtga cgccgcgcgc
    10981 ccgctcgaga aaggtctcgc aggcgaacac acgtgctgcg agcagccggc cgttgtgcag
    11041 gagtcggcgt tcgcgcatgt ctcctcctag ctccactgca tcaggcggtg cgcgatcgga
    11101 aagccgatca ccaggaaggc gcagggaaag atgcagagga tcagtggcgc gagcatcttc
    11161 accgggcttt ccatgaccat gcgctcggca cgcgcgaagc gctcctcggt acgctggtcc
    11221 acctg
//
LOCUS       DAOQVY010000074        11087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_6609_length_11087_cov_5.978970, whole genome shotgun sequence.
ACCESSION   DAOQVY010000074 DAOQVY010000000
VERSION     DAOQVY010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11087
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6609_length_11087_cov_5.978970"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PKN91_08010"
     CDS             <1..477
                     /locus_tag="PKN91_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HNS28055.1"
                     /translation="TIVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRIGA
                     LKSGEEVVGNMTRVKVVKNKVAPPFREAEFEIMYGAGISREGEILDLGSANGIIDKAG
                     AWYSYKGERIGQGKDNARTFLAQHPEIAQDIEAQIRAKLLPAKAARNGGEQAADSA"
     gene            452..964
                     /locus_tag="PKN91_08015"
     CDS             452..964
                     /locus_tag="PKN91_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNS28056.1"
                     /translation="MSRLPTPPDPLREPEARPEAAARAAAVALLARRDYASGELRARL
                     IDKGHEVPIVETLVADFVTKRLIDDARYAERYVAYKAARGQGPVRIRQDLKALGLPGE
                     LIAAALDAGPDFRRLAAAARARKFGARPPASYQDKARQARFLQYRGFSADDIRSALGN
                     DPGDFIDPDP"
     gene            961..3603
                     /gene="alaS"
                     /locus_tag="PKN91_08020"
     CDS             961..3603
                     /gene="alaS"
                     /locus_tag="PKN91_08020"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNS28057.1"
                     /translation="MTKKKPPYMSAADVRRAFLEFFRERGHTIVPSSSLVPGNDPTLL
                     FTNAGMVQFKDVFLGKDKRDYVRAASSQRCVRAGGKHNDLENVGYTARHHTFFEMLGN
                     FSFGDYFKRDAIRYAWEFVTGVLALDPARLWVTVYQDDDEAADIWLKEIGVPASRFSR
                     MGEKSNFWAMGDTGPCGPCSEIFYDHGPQIAGGPPGSADEDGDRYTEIWNLVFMQYDR
                     APDGTMTPLPKPSVDTGAGLERLSAVMQGVHNNYEIDLFRNLVAAAGELAGTQDLANS
                     SLRVIADHIRACSFLVVDGVTPSNEGRGYVLRRIVRRAIRHGYKLGIKDVFFWKLVAP
                     LEKEMGAAYPELAAARAHVERVLKLEEEQFARTLETGMGLLEAAIAKLGASRTIDGET
                     AFRLYDTYGFPVDLTADIARERGLAIDEAGFETAMEAQRERARAASKFGVDLRGGAQI
                     DARSDFLGYEGTRDTGRVVALLRSGEQVERLAAGEQGEVVLDRTPFYAESGGQVGDTG
                     TLTSQGGAQPARFAVTDTQKRGGAISHLGTLESGTLAVGDTLAADVDATRRDAIRRNH
                     TATHLLHAALRRVLGTHVQQKGSLVAPDRLRFDFAHFQPVTATELREIERLVNAEIRA
                     NAAADTKLMAYDDAVASGAMALFGEKYDSHVRVLRLGDFSTELCGGTHVARAGDIGLF
                     KIVSEGGVAAGVRRIEALTGAGAVEYVEASDDLLREVAGLVRGTREDLGEKLRGTLER
                     VRELEKEVRALKDKLASGQGTDLAAGAIDVGGVKVVATQIEGADAGALRSAVDQLKER
                     LKSAVIVLASVESPAKIVLVAGVTPDQSKRVLAGELVGAVAAKVGGKGGGRPDFAQAG
                     GTNPAALPDALGGVADFVRSKLGA"
     gene            3617..4846
                     /locus_tag="PKN91_08025"
     CDS             3617..4846
                     /locus_tag="PKN91_08025"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNS28058.1"
                     /translation="MALIVQKYGGSSVGSLELIHKVAARVAAVRGRGDRVVVAVSAMG
                     DATDRLVDLAHALCPNPDPRELDALLATGEQVSSALLAMALHARGVPARSLTGSQVPI
                     RTTSAHGRARIEEVGTAALNAVLEAGEVPVVAGFQGVDATGAVTTIGRGGSDTTAVAL
                     AVALAADECQILTDVDGVYTCDPRMVPDARRLERLSFDEMLELAGQGSRVLHLRAVEF
                     AAKYGIALRVLASHGEGPGTLIDREDPRVEAPVVSGVAFNRDEAQIVVSGVPNAPDTA
                     HRLLQGVSGAGIEVDMIVLASNQDGSIDFAFTVHRGDYARAVELTRLGAAFCPGARID
                     STDRVAKMSIVGVGMRSHAGVAARLFAALARAGITVRLVSTSEIRISVLIDEAELERA
                     VRIIHYEFSLSGHSGNY"
     gene            4915..5136
                     /gene="csrA"
                     /locus_tag="PKN91_08030"
     CDS             4915..5136
                     /gene="csrA"
                     /locus_tag="PKN91_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009847619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HNS28059.1"
                     /translation="MLILTRRVGEAVMIGDEVTVTVLGVKGNQVRIGVDAPKSVSVHR
                     EEIYERIQREEKGEGDLPAAADLPGYAVG"
     gene            5259..5349
                     /locus_tag="PKN91_08035"
     tRNA            5259..5349
                     /locus_tag="PKN91_08035"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5293..5295,aa:Ser,seq:gct)
     gene            complement(5391..6785)
                     /locus_tag="PKN91_08040"
     CDS             complement(5391..6785)
                     /locus_tag="PKN91_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28060.1"
                     /translation="MINMSAHPFPLQESIAETLNRECDCSVADLPTLRRQIEHAMEPG
                     QAIVESHPHLFSATPVFLQAAHVDQMRRVIESIEAIAHLPAYQQAVLAQAPPVAHVSS
                     RTSGAFMGFDFHITPSGPKLIEINTNAGGAFLNIAAQGSHRACCEAADGCWPLQPGLE
                     QSEAEVISMLKHEWRLARGDAELRTIAIVDENPRRQFLYPEFQLARRLFESHGLRALI
                     VDPSKLEITGNTLRSAGERVDLVYNRLTDFYFEAPGSQVLRVAYEQDLSVITPHPRAH
                     ALYANKRNLVLLSDAGELERLGAERSAIEHLGHTIPPTREVNCGDEHWWQERKGWFFK
                     PRNGFGSRGVYRGDKLTRRVFAEVANGGYLAQEFTPASERRRSVAGNPEIFKVDVRCY
                     VYAGKIQLMAARLYQGQTTNFRTAGGGFAPVYVIDEAALRPETIVGPGTSGGRCRTPC
                     RHSPPRRRSHSRFR"
     gene            complement(6782..7222)
                     /gene="arsN2"
                     /locus_tag="PKN91_08045"
     CDS             complement(6782..7222)
                     /gene="arsN2"
                     /locus_tag="PKN91_08045"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HNS28061.1"
                     /translation="MNSTPAILPNPPLTTVVRLLAAAQLPTEDLTAAHCQHFFYAESS
                     GTATGLVGLELFGDVALLRSLVVAPQRRGMGEGKALLQCAEDHARASGVHSLYLLTTT
                     AERFFAKHGYRRIPRDSAPACIRNTREFAGICPASSAFMIRDLS"
     gene            complement(7219..7527)
                     /locus_tag="PKN91_08050"
     CDS             complement(7219..7527)
                     /locus_tag="PKN91_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS28062.1"
                     /translation="MKTPQVIDALGALAHEYRLAIYRLLVEHGPDGLPAGAIGERVGL
                     VPSSLTFHLQALHRAGLITQTRASRQLIYCADYSVMNELVGYLTDKCCAASVHSRDVA
                     "
     gene            7763..8128
                     /locus_tag="PKN91_08055"
     CDS             7763..8128
                     /locus_tag="PKN91_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNS28063.1"
                     /translation="MVQYTQARFDTSFAALSDATRRGVLEQLGRADASITDLAEKFHM
                     TLTGMKKHVGVLERAGLVTTEKVGRVRTCRLGARRLEEEAAWIERYRQLWDARFCELD
                     QVIEELKRKEKAHGRRSRK"
     gene            8139..8609
                     /locus_tag="PKN91_08060"
     CDS             8139..8609
                     /locus_tag="PKN91_08060"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNS28064.1"
                     /translation="MKNHTSVARTSGRELVVTRTFDAPARLVFEAWTRPELLRKWWVP
                     KSIDIALLSCEVDARVGGRYRFEFAGGEAGPMVFFGRYLEVTPHARLVWTNEESEAGS
                     VTTLTFEESNGRTLVVVHELYPSKEALDAAGTGSADALVETFRQLDELLVTLGG"
     gene            complement(8613..9893)
                     /locus_tag="PKN91_08065"
     CDS             complement(8613..9893)
                     /locus_tag="PKN91_08065"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNS28065.1"
                     /translation="MSAVSALLRRLACPLALLCVVTGAYAQDLLIRGAHVHTASTAGD
                     IPGGDVLVRNGKIAAVGTGIAIPSGVQVVEAGGKPLTPGLFAGITAIGVEEVSLESGT
                     VDEYLTLGAPAWQMQWRPEFDVTLAFNPRSVLLPVARIEGLTWAIVAPDSRNGGAILS
                     GQGAAMTLDGRYDAVLDGSRSLFMQFGADAVNLSAGSRAAQYMLFDQAAREARSPPAA
                     GAQAMLLPAGREVLARYLDGGRFVFDVHRAADIRQVLALAKRHSIRPVIVGASEAWLV
                     AGELAAARVPVLLDPLENLPATFDRIGARFDNAALLAKAGVAVAFALSSDSTQNARKI
                     RQLAGNAVAHGLPWDVALAGLTSTPADIFGVARSRGRIAVGQVADLVLWSGDPLEVTS
                     VAEQVWTAGTPVVMRSRQTELRDRYLERLRGGED"
     gene            complement(9890..>11087)
                     /locus_tag="PKN91_08070"
     CDS             complement(9890..>11087)
                     /locus_tag="PKN91_08070"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNS28066.1"
                     /translation="EAVGRNLAVPAGATEIDARDRWVTPGLIDVHSHLGVYASPDMDA
                     HADGNEMIAPVTANVWAEHGVWPQDPGFEAALEGGVTTLQVLPGSANLIGGRGVTLKN
                     VAATGVQGMKFPDAPYGLKMACGENPKRVYGSKSQAPKTRMGNIAGYRQAFADAQEYQ
                     RQWEKYARELAAHETKKDKDDDAQPPSEPKRDLGKETLVEVLEGRILVHIHCYRADEM
                     QLMLDLADEFGFRIAAFHHGVEAYKIADTLAARQVCGALWADWWGFKMESFDGIQENL
                     ALVDRPQGSCAIVHSDSSEGIQRLNQEAAKAMTRGVRLGFDIPPERAIRWLTAHAARA
                     LGVADRTGTLESGKMADVVIWDRDPFSVYARAQQVFVDGVLAFDRAHPRPPRSDFMLG
                     QQGDAP"
BASE COUNT         1879 a         3683 c         3760 g         1765 t
ORIGIN      
        1 accatcgtca tcttcatcaa ccagatccgc atgaagatcg gcgtcatgtt cggcagcccg
       61 gaaaccacca cgggcggcaa tgccctcaag ttctatgcct ccgtgcgcct cgacatccgc
      121 cgcatcggcg cgctgaagag cggcgaggag gtcgtcggca acatgacccg cgtcaaggtc
      181 gtgaagaaca aggtcgcgcc gcctttccgc gaagccgaat tcgagatcat gtacggcgcc
      241 ggcatttcgc gcgagggcga gatcctcgac ctcggcagcg ccaacggcat catcgacaag
      301 gccggcgcct ggtacagcta caagggcgag cgcatcgggc agggcaagga caacgcccgc
      361 accttcctcg cgcagcatcc ggagatcgcg caggacatcg aggcgcagat ccgcgccaag
      421 ctgctgccgg ccaaggccgc gcgcaatggc ggtgagcagg ctgccgactc cgcctgaccc
      481 tctccgcgag ccagaagcca ggccggaggc ggcggcccgc gcggcggcgg ttgcgctgct
      541 ggcgcgccgc gactacgcct cgggcgaatt gcgggccagg ctgatcgaca agggccacga
      601 agtgcccatc gtcgagacgc tggtggccga cttcgtgacc aagcgcctga tcgacgacgc
      661 ccgctacgcg gagcgctacg tggcctacaa ggccgcgcgc ggacagggcc cggtgcgcat
      721 ccgtcaggac ctcaaggccc tcggcctgcc gggcgagctg atcgccgccg cactcgatgc
      781 cggccccgat ttccggcgcc tggccgccgc cgcccgggcg cgaaagttcg gcgcccgccc
      841 gcccgccagc taccaggaca aggcccgcca agcccgtttt ttgcaatatc ggggcttttc
      901 cgccgatgat atccggtcag ccctcgggaa cgaccccggg gacttcatcg acccggatcc
      961 atgaccaaga aaaaaccgcc ttacatgagc gcggcggatg tccgacgcgc cttcctcgag
     1021 tttttccgcg aacgcggcca cacgatcgtg ccgtcctcct cgctggtgcc cggcaacgac
     1081 ccgacgctgc tgttcaccaa tgccggcatg gtgcagttca aggatgtgtt cctcggcaag
     1141 gacaagcgcg actacgtgcg cgccgcctcc agccagcgct gcgtgcgcgc cggcggcaag
     1201 cacaacgacc tcgagaacgt cggctacacc gcgcgccacc acaccttctt cgagatgctc
     1261 ggcaacttct cgttcggcga ctatttcaag cgcgatgcga tccgctacgc gtgggaattc
     1321 gtcaccggcg tgctcgcgct cgaccctgcg cgcctgtggg tgaccgtcta ccaggacgat
     1381 gacgaggcgg ccgacatctg gctgaaggag atcggcgtgc cggcgagccg gttctcgcgc
     1441 atgggcgaga agtcgaactt ctgggcgatg ggcgacaccg ggccctgcgg cccgtgcagc
     1501 gagatcttct acgatcacgg cccgcagatc gccggcggtc ccccgggctc ggccgacgag
     1561 gacggcgatc gctacaccga gatctggaac ctcgtgttca tgcagtacga ccgagccccg
     1621 gacggcacga tgacgccgct gccgaagcca tcggtcgaca ccggcgccgg actcgagcgc
     1681 ctgtcggccg tgatgcaggg cgtgcacaac aactacgaga tcgacctgtt caggaacctg
     1741 gtcgcggcgg ccggcgagct cgccggcacg caggacctcg cgaacagttc gctgcgcgtg
     1801 atcgccgatc acatccgtgc ctgctcgttc ctggtcgtcg acggggtcac gccgtcgaac
     1861 gaggggcgcg ggtacgtgct tcgtcgcatc gtgcgccggg cgatccgcca cggctacaag
     1921 ctcggcatca aggatgtgtt cttctggaag ctcgtcgcgc cgctcgagaa ggaaatgggt
     1981 gcggcgtatc ccgaactcgc cgccgcgcgc gcccacgtcg agcgcgtgct gaagctcgag
     2041 gaagagcagt tcgcgcgcac gctcgagacc ggcatggggc tgctcgaggc ggcgatcgcg
     2101 aagctcggtg cgtcgcgcac gatcgacggc gagaccgcct tcaggctcta cgacacctac
     2161 ggcttcccgg tggacctcac ggccgacatc gcgcgcgaac gcggccttgc gatcgacgag
     2221 gcgggcttcg agaccgccat ggaagcgcag cgcgagcgcg cccgcgcggc cagcaagttc
     2281 ggcgtcgacc tgcgcggcgg tgcgcagatc gatgcgcgtt cggacttcct cggctacgag
     2341 ggcacgcgcg acaccggcag ggtcgtggcg ctgctgcgct cgggcgagca ggtcgaacgc
     2401 ctcgccgccg gcgagcaggg cgaggtcgtg ctcgaccgca cgcccttcta cgcggagtcg
     2461 ggcggccagg tgggcgatac cggcacgctc acctcgcagg gcggtgcgca gcccgcgcgc
     2521 ttcgccgtca ccgataccca gaagcgcggt ggcgcgatct cgcacctcgg cacgctcgag
     2581 tcgggcacgc tcgcggtggg cgatacgctg gccgccgacg tcgatgccac gcggcgggat
     2641 gcgatccggc gcaaccacac ggccacgcac ctgctgcacg ccgcgctgcg cagggtgctc
     2701 ggcacacacg tccagcagaa gggctccctg gtcgcgcccg accgcttgcg cttcgacttc
     2761 gcgcacttcc agccggtaac cgcgaccgag ctgcgcgaga tcgaacgact cgtgaatgcc
     2821 gagatccgcg ccaacgcggc cgcggacacg aagctcatgg cctatgacga cgcggtcgcc
     2881 tccggggcga tggcgctgtt cggcgagaaa tacgacagcc acgtgcgcgt attgcggctc
     2941 ggggattttt cgaccgagct gtgcggcggc acccacgtgg cgcgggcggg cgacatcggc
     3001 ctcttcaaga ttgtcagcga gggcggggtc gccgcgggcg tgcgccgcat cgaggcgctg
     3061 accggcgccg gggcggtcga atacgtcgag gcgagcgacg acctgctgcg cgaggtcgcg
     3121 ggcctcgtgc gtggcacgcg cgaggatctc ggcgagaagc tgcgcggcac gctcgagcgc
     3181 gtgcgcgaac tcgaaaagga agtgcgtgcg ctcaaggaca agctggcctc ggggcagggc
     3241 accgacctcg cggctggcgc catcgacgtc ggtggcgtga aggtcgtcgc cacgcagatc
     3301 gagggcgccg acgccggcgc gctgcgcagc gcggtcgacc agctgaagga gcggctcaag
     3361 tcggcggtca tcgtgctcgc ctcggtggag agtcccgcga agatcgtgct ggtcgccggc
     3421 gtcacgcccg accagtcgaa gcgcgtgttg gcaggcgaac tcgtcggcgc cgtcgccgcg
     3481 aaagtcggcg gcaagggcgg cggacggccc gatttcgcgc aggccggcgg caccaacccc
     3541 gccgcactgc ccgacgcgct cggcggcgtg gccgacttcg tgcgcagcaa gctcggcgcg
     3601 taacccgccg ccgtccgtgg cgctgatcgt ccagaagtac ggtggcagct cggtcggctc
     3661 gctcgagctg atccacaagg tcgccgcacg cgtggcggcg gtgcgcgggc gcggcgaccg
     3721 ggtggtcgtg gccgtttcgg cgatgggcga tgcgaccgat cgtctcgtcg acctcgcgca
     3781 tgcgctgtgc cccaaccccg atccgcgcga actcgatgcg ctgctcgcga ccggcgagca
     3841 ggtctccagc gcgttgctcg cgatggcgct gcatgcgcgc ggcgtgcccg cccgctcgct
     3901 gaccggttca caggtcccca ttcgcacgac cagcgcgcat ggccgcgcgc gcatcgagga
     3961 agtcgggaca gccgcgctca acgcggtgct cgaagccggc gaggtgccgg tggtggccgg
     4021 cttccagggc gtcgatgcga cgggcgccgt caccacgatc gggcgcggcg gctcggacac
     4081 gacggcggtc gcgcttgccg tcgcgcttgc cgccgacgag tgtcagatcc tgacggacgt
     4141 cgacggcgtc tacacctgcg acccgcgcat ggtgccggac gcgcgccggc tcgagcggct
     4201 gtcgttcgac gaaatgctcg aactcgcggg gcagggctcg cgcgtactgc atctgcgggc
     4261 ggtggaattc gccgcgaaat acggcatcgc gctgcgtgtg ctcgcgagcc acggggaagg
     4321 ccccggaacc ttgatcgatc gggaggatcc acgcgtggaa gcaccggtag tttcgggcgt
     4381 cgcattcaat cgcgacgagg cgcagatcgt ggtgagcggc gtgccgaatg cgcccgacac
     4441 cgcgcatcgg ctgctgcagg gcgtgtcggg ggcgggcatc gaagtcgaca tgatcgtgct
     4501 cgcctcgaat caggatggtt cgatcgactt cgcgttcacc gtgcaccgcg gcgactacgc
     4561 gcgcgcggtc gaactcacgc gtctcggtgc cgcgttctgc ccgggcgcac gcatcgacag
     4621 caccgaccgg gtcgcaaaaa tgtcgatcgt cggcgtcggc atgcgctcgc acgccggggt
     4681 cgcggcgcgc ctgttcgcgg cgctcgcccg tgcaggcatc acagttcgcc tcgtttcgac
     4741 gtcggaaatc cgcatatccg tgttgatcga cgaggcggag ctcgagcgtg ccgtacgtat
     4801 aatccactac gaattttcgc tgtcgggaca ttcagggaat tactgatgtg cgcggacagg
     4861 gtcagcggct acattgatct ccagattgcc tttttccatg gaggtccaaa gagaatgctg
     4921 attctgacaa gacgcgttgg cgaagccgtg atgatcggtg acgaagtgac cgtcacagtg
     4981 ctcggagtga aaggcaacca ggttcgcatc ggcgtggacg cgccgaagtc cgtgtccgtg
     5041 caccgcgaag agatctacga gcgcatccag cgcgaagaaa agggcgaggg cgacctgccc
     5101 gccgccgccg atctgccggg ctacgcggtc ggctgatccc gcccgggccg gctgtctggc
     5161 ccgccccccg agtttccagc aaggccccgc cgccccgagc gaccgggcct tttgcctgtc
     5221 cggaccccgg ccagtatcat gcgccccttc cacgcgccgg agagatggcc gagtggtcga
     5281 aggcgcaccc ctgctaaggg tgtagggacc aaaagttcct cgagggttcg aatccctctc
     5341 tctccgccaa ttaccttgga cgccgcgccc ggctggcgcc atgtgccgta ttaccgaaaa
     5401 cggctgtgcg agcgacgacg tggtggcgaa tgacggcatg gtgtacgaca ccggccacct
     5461 gatgttcccg gcccgactat cgtttcgggc cgcagcgctg cttcatcaat gacgtaaacc
     5521 ggcgcaaaac caccgcccgc agtccggaag ttggtggtct ggccttgata cagtctcgcg
     5581 gccatcagct gaatcttgcc ggcatacacg tagcagcgta cgtccacctt gaagatctcc
     5641 gggttgccag cgacggagcg gcgccgttcg cttgccggcg tgaactcctg agcaaggtac
     5701 ccaccattcg cgacttcggc gaacacgcgg cgcgtgagct tgtcgccgcg gtagacaccg
     5761 cgactgccaa agccgttacg cggtttgaag aaccagccct tgcgctcctg ccaccagtgt
     5821 tcatcgccgc agttcacttc ccgcgtcggt gggatcgtgt ggcccagatg ctcgatggcc
     5881 gaccgttccg ctcccaggcg ctcgagttcg ccggcatcgc tgaggagaac aaggtttcgc
     5941 ttgttcgcgt agagcgcgtg ggcgcgagga tgcggagtaa ttacggaaag gtcctgctca
     6001 tacgcgacac gcaggacctg gctgccgggc gcttcgaagt agaagtcggt gagacggttg
     6061 tagacgagat ccacgcgttc gccggcggat ctcaacgtgt tgccggtgat ctcgagtttt
     6121 gacgggtcga cgatgagggc acgcaatccg tgcgattcga acagccgcct ggccagctgg
     6181 aattcgggat acaggaattg ccgacgcggg ttctcgtcaa cgatggcaat tgtccgcagt
     6241 tctgcgtctc cccttgccag tctccattcg tgcttgagca tcgatatgac ttccgcctcg
     6301 gactgctcca acccgggctg aagcggccag cagccgtccg cggcttcgca acaagcgcga
     6361 tgtgagccct gcgcggcgat attcagaaac gcgccgccgg cgttggtatt gatttcgatg
     6421 agcttcgggc cgctcggcgt gatgtggaaa tcgaatccca tgaaagcgcc agatgtacgc
     6481 gagctcacgt gcgcgacggg cggcgcttgc gccagaaccg cctgttgata ggccggcaga
     6541 tgtgcgatcg cttcgatcga ttcgatgaca cgccgcattt ggtcgacgtg agcggcttgc
     6601 agaaagaccg gtgtcgcgct gaaaagatgc ggatgactct cgacgatcgc ctgccccggt
     6661 tccatcgcgt gctcgatctg ccttcgcagc gtcggcagat ccgcaacgct gcaatcgcat
     6721 tcccggttga gcgtttcggc aatgctctcc tgcaggggga aagggtgggc ggacatgttg
     6781 atcaactgag atcgcggatc atgaatgcgg aactcgcggg gcagataccc gcgaattccc
     6841 gcgtgttgcg aatgcaggct ggtgcggagt cgcgtgggat tcgacgatag ccatgcttcg
     6901 cgaaaaaccg ctcggctgtc gtcgtgagga gatagagcga gtgcacgcca ctggcgcggg
     6961 catggtcctc cgcacattgg aggagcgcct tgccttcacc catcccgcgc cgctgaggcg
     7021 ccacgacaag agagcgcagc agggccacgt cgccaaagag ttcgagcccg acgagaccgg
     7081 tcgccgtgcc agaggattcg gcatagaaga agtgctggca atgcgcggca gtcaggtctt
     7141 cggttggcaa ctgcgcggcg gcgagaagcc tcaccacggt agtcagcggc ggattcggca
     7201 ggatggcggg cgtggaattc atgcgacatc gcggctgtga acgctggccg cgcagcattt
     7261 gtcagtgaga tagccgacga gctcgttcat gacggaatag tcggcacagt agatgagctg
     7321 ccgactggcg cgggtctgtg tgatcagccc ggcccgatgc agggcttgaa gatgaaaggt
     7381 gagcgaggag gggacgagcc cgacgcgttc gccaattgca cctgccggca agccgtcagg
     7441 cccgtgctcc accagcagac ggtagatcgc caggcggtac tcatgcgcaa gggcgccaag
     7501 ggcgtcgatg acttgagggg ttttcacatc gcgtacgata ttacgggaag cgtcgaaatg
     7561 tcaacccaga tggcaagttg ccggggagcg gtatgaatct ctgtccgcca gctttcaggc
     7621 atgtgatcgg cgctgcggcc gccgccgcgc acccgccttc cgccccggtg aagaaagcgc
     7681 cggcgtcctg agtcggcgtg caccacgatc cggccagtca ccccttgcgg gccgccaggc
     7741 gctgtgttaa ttactgaact atatggttca gtatacacag gcccgcttcg atacctcgtt
     7801 cgccgcactc tcggacgcca cccgccgcgg cgtcctcgag cagctcgggc gtgccgacgc
     7861 ctcgatcacg gacctggccg agaagttcca catgaccctc acgggcatga agaagcatgt
     7921 cggcgtactg gagcgggcgg ggctcgtcac cacggagaag gtcgggcgcg tgcggacctg
     7981 caggctcggc gcgcgccgac tggaagaaga ggcggcatgg atcgagagat accgccaact
     8041 ctgggacgcc cgcttctgcg agctggacca ggttatcgag gaattgaaac gcaaggagaa
     8101 ggcgcatgga cgcagaagca ggaagtaagc ccgcatcgat gaagaatcac acgagcgtcg
     8161 cgcgcacgtc cgggcgcgag ctcgtcgtca cgcgaacctt cgacgccccg gcgcgcctgg
     8221 tgttcgaggc ctggaccaga cccgagctgc tcaggaagtg gtgggtgccg aagtcgatcg
     8281 acatcgctct gctgtcgtgc gaggtggatg cccgggtcgg gggccgatat cgcttcgagt
     8341 tcgccggcgg tgaggcaggg cccatggtgt tcttcggccg ctacctcgaa gtgacgccgc
     8401 atgcgcgcct cgtgtggacc aacgaggaaa gcgaggccgg cagtgtcacc acgttgacct
     8461 tcgaggagag caatggcagg actttggtgg tcgtgcacga gctctatccc tcgaaggaag
     8521 ccctcgatgc ggccggcacc ggctcggcgg atgcgctggt cgagactttc aggcagctcg
     8581 acgaactgct cgtcaccctg ggtggttgac cgtcagtcct cgccgccgcg caaccgctcg
     8641 aggtagcgat cgcgcagctc ggtctggcgc gagcgcatga cgacgggagt gcccgctgtc
     8701 cagacttgct cggcgacgct cgtgacctcc agcggatcgc cgctccacag cacgaggtcg
     8761 gcgacctggc ccaccgcgat gcgaccgcgg ctcctcgcga cgccgaagat gtccgcgggc
     8821 gtcgaggtca aaccggccag cgcgacgtcc cacggcaggc cgtgcgccac ggcattgccg
     8881 gcgagctggc ggatcttgcg cgcgttctgg gtcgaatcag acgacagcgc gaacgccacc
     8941 gcgacgccgg ccttcgcgag cagcgcggca ttgtcgaagc gcgcgccaat gcgatcgaat
     9001 gtcgccggca gattctcgag cggatcgagc agcaccggca ccctggctgc ggcaagttca
     9061 cccgcgacca gccaggcttc gctcgcgccg acgatcaccg gcctgatgct gtgacgcttg
     9121 gccagcgcga gcacctggcg aatgtcggcg gcacgatgca catcgaatac gaaacggcca
     9181 ccgtcgagat agcgcgctag cacttcgcgt cccgcgggca gcagcatggc ttgtgcgccg
     9241 gcggcgggcg gtgaccgggc ctcgcgggcg gcctggtcga acaacatgta ctgcgcggcg
     9301 cgactgcccg cggacaggtt cacggcatcg gcgccgaact gcatgaacag cgagcgactg
     9361 ccgtcgagca cggcgtcgta gcggccatcg agcgtcatcg ccgcgccctg ccccgagaga
     9421 atcgccccgc cgttgcggct atccggcgcg acgatcgccc aggtcagtcc ctcgatacgc
     9481 gccaccggca gcagcacgga gcgcgggttg aacgcgaggg tcacgtcgaa ctcggggcgc
     9541 cattgcatct gccacgcggg tgcgccgagg gtcaggtact catcgacagt gccgctctcg
     9601 agcgagacct cctcgacgcc gatggccgtg atgccggcga agagtcccgg cgtcagcggc
     9661 ttgccgcctg cttcgacgac ctgcacgcca gacgggatgg cgatgccggt gcccaccgcc
     9721 gcgattttgc cgttgcgcac gagaacgtcg cctccgggga tgtcgccggc cgtcgaggcc
     9781 gtgtggacgt gggcgccgcg gatcagcaga tcctgggcgt acgccccggt gaccacgcac
     9841 agcaacgcga gcgggcaggc gaggcggcgc aggagggcac tgaccgcgct cacggcgcat
     9901 ctccctgctg tcccagcatg aagtcggaac gtggcggtcg cggatgcgcc cggtcgaacg
     9961 cgagcacacc gtcgacgaag acctgctgcg cgcgcgcata gacgctgaac gggtcacggt
    10021 cccagatcac cacatccgcc atcttgccgg actcgagcgt gccggtgcga tcggccacgc
    10081 cgagcgcccg ggcagcatgc gccgtcagcc agcggatggc gcgctcggga ggaatgtcga
    10141 agccgagacg gacgcccctc gtcatcgcct tggcggcctc ctggttcagg cgctggatgc
    10201 cttccgacga atcggaatgc acgatggcgc aactgccttg cggccggtcg accagcgcca
    10261 gattctcctg gatgccgtcg aaggactcca tcttgaatcc ccaccagtcg gcccacaacg
    10321 cgccgcagac ctgccttgcg gcaagcgtat cggcgatctt gtacgcctcg actccgtggt
    10381 ggaacgcggc gatccggaag ccgaactcgt cggcaaggtc cagcatcaac tgcatctcgt
    10441 cggcgcgata gcagtggatg tgcacgagga tccggccctc cagcacctcg accagcgtct
    10501 ccttgccgag atcgcgcttg ggctccgagg gcggctgcgc atcgtcgtcc ttgtccttct
    10561 tcgtttcatg cgccgcaagt tcgcgcgcgt acttctccca ttggcgctga tactcctgtg
    10621 cgtcggcgaa ggcctggcga taacctgcga tattgcccat gcgggtcttc ggcgcctgcg
    10681 acttgctgcc atagacgcgc ttgggattct cgccgcacgc catcttcaat ccatacgggg
    10741 catccggaaa cttcatgccc tgcacaccgg tggcggcaac attcttcagg gtgacaccgc
    10801 gaccgccgat gaggttggcc gagccgggca gcacctgcag cgtggtgaca ccgccctcga
    10861 gcgcggcttc gaagcccggg tcctgcggcc acacgccatg ctcggcccag acattggcgg
    10921 tcacgggcgc gatcatctcg ttgccgtcgg catgggcgtc catgtccggg ctcgcgtaca
    10981 caccgagatg cgagtgaacg tcgatgagcc ccggcgtgac ccagcgatcc cgcgcatcga
    11041 tctcggtggc gccggcaggc accgcgagat tgcgccccac ggcttcg
//
LOCUS       DAOQVY010000075        10986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_6697_length_10986_cov_5.223767, whole genome shotgun sequence.
ACCESSION   DAOQVY010000075 DAOQVY010000000
VERSION     DAOQVY010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10986
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6697_length_10986_cov_5.223767"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PKN91_08075"
     CDS             complement(<1..256)
                     /locus_tag="PKN91_08075"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNS28067.1"
                     /translation="MRGPVNIVGAGLAGTLLAIVLARRGFRVEVFERRADPRLRAEER
                     GRSINLALAARGLRGLEVAGVAQRVAPLLIPMRGRLVHDHD"
     gene            complement(264..1484)
                     /gene="kynU"
                     /locus_tag="PKN91_08080"
     CDS             complement(264..1484)
                     /gene="kynU"
                     /locus_tag="PKN91_08080"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HNS28068.1"
                     /translation="MRERFELPRDASGEPLVYFCGHSLGLMPIAARRIVNEELDSWAA
                     RGIDGQFVGQRPWVDYASLMQAGLAALAGAETGEVVAMNALTVNLHVLMASFYRPQGA
                     RRRILIEAGAFPSDRHAVAGQIAWHGLDPRECLVELAPRAGEELLREDDIDDAIGRAG
                     DTLALVLWPGVQYLTGQAFDCARIARAARRAGAAVGFDMAHAIGNLPLALHDWDADFA
                     AWCSYKYLNAGPGAVGGAFVHARYAQRSDLPRLAGWWGHDPATRFLMAPRFDAAAGAA
                     GWQVSNPPIFSSAPLLASLEIFMTAGIARLREQSIGLTRALEAEIDALPAGEVHLITP
                     RDARARGAQLSLRVRGGDRRGRRVFDALAARGIVCDWREPDVLRIAPVPLYNTPDEVR
                     RAGAALRSALATVG"
     gene            complement(1484..2290)
                     /locus_tag="PKN91_08085"
     CDS             complement(1484..2290)
                     /locus_tag="PKN91_08085"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNS28069.1"
                     /translation="MKASFAVGSSELRVDLAQAVELARPLGFDGASARHFAAPAAEAR
                     PFSIGDFSGDVATGASCNCRTITLTPHCNGTHTECVAHLTVEALDAWRVVPRAPVAAI
                     VVSVVPDRIGEDRVITRAALAAAWQCADVPPGPVVPQALILRTLPDPNDAGATAPGSM
                     APYVAADAASWIVGLGIAHLVLDLPSLDRTHDGGLLAAHRIFFGLPPKSRRLADAARA
                     YATVTELAHIPDSLADGPCLLAWQVPAMAGDAVPSRPLAYRYETIHGPVT"
     gene            complement(2287..2814)
                     /locus_tag="PKN91_08090"
     CDS             complement(2287..2814)
                     /locus_tag="PKN91_08090"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF009763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HNS28070.1"
                     /translation="MTQLKAFSLKSWIDAHRDDLKPPVGNRRLFREGDFIIMVVGGPN
                     QRNDFHLDPGEEFFFQLEGDMLLKVVQDGRIVDVPIREGEVLLLPAHVPHSPQRFPDT
                     VGLVIERVRRPGELDGLAWFCDNCHAELYREEFELTDIETQFPPVFERYYASLAHRSC
                     RRCGTVQQPPAARRS"
     gene            2851..3375
                     /locus_tag="PKN91_08095"
     CDS             2851..3375
                     /locus_tag="PKN91_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28071.1"
                     /translation="MVRNRAWIGVGLAAAMVAGCGGMPQLSWPWARAGAPAPQPVDEL
                     TFAGDATAPIAQHWQGNTLVLDFTTVPAEGQASATPTHARGWPLRMAVRTWPGRFGAL
                     EVRGAQRVLLPLTTDGSDMVDVAIPPEVFAAGTPQIELRWGAAIAPPVVFPQAPPPAP
                     AAASAATDQSAPMR"
     gene            complement(3354..4193)
                     /locus_tag="PKN91_08100"
     CDS             complement(3354..4193)
                     /locus_tag="PKN91_08100"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HNS28072.1"
                     /translation="MARYAIGDIQGCYDELRALLRALRFSSDRDELWFVGDLVNRGPK
                     SLEVLRFVRDLGGNAVTVLGNHDLHLLAVAFGHGSRRLRRGDTLDAVLEASDREQLLE
                     WLLAQPLAHHDADRGDLLVHAGLVPQWRAVDAVRLAREVGAALAADAPAVFAGMYGNQ
                     PDTWDENLEGMARLRFIINALTRLRFCTPGGRMDLAEKAAPGDVAPPLAPWFSYGSAR
                     TRGARVICGHWSALGYSDSNGVIAIDTGCVWGGALTALSLDDPQRLPLQVACGAYQRI
                     GAD"
     gene            complement(4199..5020)
                     /gene="apaG"
                     /locus_tag="PKN91_08105"
     CDS             complement(4199..5020)
                     /gene="apaG"
                     /locus_tag="PKN91_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNS28073.1"
                     /translation="MDAYRRILLPVDLTEDSTPVAERAKFLAETFHADLELLHVVEFV
                     PVEPMGETLMPAVAIEEELVQRARQRLTALAGSLGLAEAPVHVEAGNVKAEIIRVARE
                     RNVDLIVLGSRERHGLSILVNLTEDAVLHGAPCDVLAVRVGPRGPMSSAPHRIRVDVE
                     TVYLDERSDPGEHRYVFAYTITVRNDGTTPARLLARHWLITDANGKVQEVRGDGVLGE
                     QPYLKPGQGFRYSSGTMIETPVGAMQGSYQMVDDEGLAFDAPIAPFRLAIPGVLH"
     gene            complement(5030..5809)
                     /gene="rsmA"
                     /locus_tag="PKN91_08110"
     CDS             complement(5030..5809)
                     /gene="rsmA"
                     /locus_tag="PKN91_08110"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNS28074.1"
                     /translation="MPAPARKRFGQHFLHDPAIVDRIVQAIDPQPGEPIVEIGPGRGV
                     LTRRLLAAARHIDAIEIDRDLAQGLAREFPPEAGLTLHVGDALAFDFAALAARRGARL
                     RIVGNLPYNISTPLLFHLLATPAAIADLHVMLQREVIDRMAAAPGHKEYGRLTVMLAP
                     WVRIERLFDVGPGAFQPPPRVWSAVARLTVRETPAFAIAPAFPRVVALAFSQRRKTLR
                     NALRELLDEAGIEAAGVDPGARAETVAPEGFGALARIAQGR"
     gene            complement(5811..6797)
                     /gene="pdxA"
                     /locus_tag="PKN91_08115"
     CDS             complement(5811..6797)
                     /gene="pdxA"
                     /locus_tag="PKN91_08115"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNS28075.1"
                     /translation="MSRLPRIALTSGEPAGIGPELCATLARSAWPCELVCFGDRALLA
                     ERAASTGNSLVLHDYARDQQRAHVPGELRVAHCPLGAPSQPGHLERRNASYVLSLLDA
                     AMAGAVTREFDAIVTAPVHKGIINDAGIAFTGHTEYFAAHGGITRPVMLLVAGGLRVA
                     LATTHLPLRAVSHAITPALLREVLDVLATDLTRLFGIAHPRILVCGLNPHAGEGGHLG
                     SEEIDVIAPALADARARGLDVKGPVPADTAFVPAQLAGVDAVLAMYHDQGLPVLKHAG
                     FGRAVNVTLGLPVIRTSVDHGTALDLAGTGRADAGSLEAALALAIEMANRRH"
     gene            complement(6802..8103)
                     /locus_tag="PKN91_08120"
     CDS             complement(6802..8103)
                     /locus_tag="PKN91_08120"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNS28076.1"
                     /translation="MLRPFLAGLLAILAAGASHAQTRELSAAGELVDRVAAIVNDGVV
                     LRSELDEQTAQIGARLRQQGLELPPENVLRQQILERLVIQEVQMQRAKRAGLNVTDEM
                     VNNALKGVAERNGIPLAQLPQVLASQGVDYAAYRDSLRKEITLSMLRQRDVIARIVVT
                     PRELDQYLEHQKNAPSESTAYNLSHILIAVPQAATPEQMAAAEQRAEDVHRRAVAGED
                     FAQLAVTYSNSQTALEGGALGWRQGPELPTVLAEAVIKLQAGEVSDVIRTPSGFHLVR
                     VNEVQSTDGPSIVQQVHARHILMKPNELQDDATVEQRMNSIRDRIMRGEDFGGIATIV
                     SEDPGSASEGGDLGWTSPGTFVPEFEAQLAALADGEISQPFRTQYGWHIVQRLGQREI
                     DNSEEVKRQRAYVALRDSKAEEETELWLRRLRDEAYVELKM"
     gene            complement(8121..10361)
                     /gene="lptD"
                     /locus_tag="PKN91_08125"
     CDS             complement(8121..10361)
                     /gene="lptD"
                     /locus_tag="PKN91_08125"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HNS28077.1"
                     /translation="MPSPRVHWSGAAVIGTLLLQAAAHAADPEPALCFGALADSGGLP
                     QGASPVPRSAEELRNEPIEVSSDDASLGVDGDAVLAGNVLVTQGDRRITADSVAYDAD
                     AGKLSVDGNVEYRDPLLTVRGSSGQYSATEGATFSGAEFLLPSRPARGTAGAMTLDTG
                     GHIGLERVTFTTCPLTDPAWQLRADRIDLDTAARNGVGRGTRVEFKGVPIFYTPWISF
                     PLGSDRKSGFLFPGAGYSSRSGAQLSVPYYWNIAPNRDFTFEPVYFARRGVDLSGDFR
                     YLTRRSRGSLDVSYLPDDQLFDGDRSLVKLVHRTELPRGWRFTIDAANASDTQYFEDF
                     GQGPEGTSITFVERGAELTYRDANWRLRGAFQDFQTIDTDVAADDRPYKMLPQLYASA
                     DFRIGQSLQLRYGFDAEVIKFDRNTGVTGWRYDAAPHIGLDWQGAGYFLRPAVSWRST
                     GYSLEDTAPGIDDSPRRNLPTVSLDAGLIFEGAAGSRNQRRVTLEPRALYVYTPYRNQ
                     DDLPVFDTAVPDLNLVQLFRTNRYVGGDRVSDADQASFGITSRILDSASGRQLLAATV
                     GQTVYFDAPRVALPGEVIVDRDTSDVIAQLAITAWQNWNADVGVQWNPETSERERMHV
                     QLQYRPASTSVVNLGYRYQRDRVDQAEASLAWPVSDRWSLFGRYVYSMQESDALDQFA
                     GFEYRSCCWRFRAVGRRFVSSRTGERDTGIYLQLELSGLASVGSSAVAFLEEAIRGYS
                     PANPSP"
     gene            10386..>10986
                     /locus_tag="PKN91_08130"
     CDS             10386..>10986
                     /locus_tag="PKN91_08130"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNS28078.1"
                     /translation="MTENDARAAQLQEWLTAGLGRPIERLTLASADASFRRYFRVHAD
                     GVSWIAMDAPPGREDVRPYLRVARLLEETGVHVPHVHAFDIEQGFVLLEDLGDTPYLT
                     QLARGEDVERLYADALAALAAIQVRGREAARELPPYDDQTLQRELDLMPEWFCARHLR
                     LALDDGELGLIVRTFDFLIEEALAQPRVFVHRDYHSRNLM"
BASE COUNT         1665 a         3715 c         3947 g         1659 t
ORIGIN      
        1 cgtcgtgatc gtgtaccagc cgcccgcgca tcggaatgag cagcggggcc acgcgctgcg
       61 cgacgccggc gacttcgagg ccgcgcaggc cgcgcgcggc gagtgcgagg ttgatcgagc
      121 ggccgcgctc ctccgcgcgc agccgcggat cggcgcggcg ctcgaacacc tcgacgcgaa
      181 agccgcgccg cgcgagcacg atcgcgagca gcgtgccggc aagtcccgcc ccgacgatgt
      241 tcaccggacc gcgcatcgcc gcctcagccg accgtcgcga gggccgagcg tagcgcggcg
      301 ccggccctgc gcacctcgtc cggcgtgttg tagagcggca ccggcgcgat gcgcagtacg
      361 tcgggctcgc gccagtcgca cacgatgccg cgtgccgcga gcgcatcgaa aacgcgccgg
      421 ccgcgccggt cgccgccgcg cacgcgcagc gagagctgtg caccgcgtgc ccgcgcatcg
      481 cgtggcgtga tgaggtggac ttcgccggcg ggcagggcgt cgatctcggc ctcgagcgcg
      541 cgcgtcaggc cgatggactg ctcgcgcagg cgagcgatgc ccgcggtcat gaagatctcg
      601 agcgaggcga gcagcggtgc gctcgagaag atcggcgggt tgctgacctg ccagccggcg
      661 gcgcctgccg cggcatcgaa gcgcggcgcc atgaggaatc gcgtcgccgg atcgtggccc
      721 caccagcccg cgaggcgtgg caggtcgctg cgctgcgcgt agcgcgcatg cacgaacgcg
      781 ccgccgaccg cgccggggcc cgcattgagg tacttgtagc tgcaccacgc ggcgaagtcg
      841 gcatcccagt cgtgcagcgc gagcggcagg ttgccgatcg catgggccat gtcgaagccc
      901 accgctgcgc cggcacgccg cgccgcgcgt gcgatgcgcg cgcagtcgaa ggcctggccg
      961 gtgaggtact gcacgccggg ccacaggacg agcgcaagcg tatccccggc acgcccgatg
     1021 gcgtcatcga tgtcatcctc gcgcagcagc tcctcgcccg cgcgcggggc gagttcgacc
     1081 aggcattccc gcgggtcgag gccatgccag gcgatctgcc cggcgacggc gtggcgatcc
     1141 gaagggaagg caccagcctc gatcaggatg cggcggcggg caccctgcgg acggtagaac
     1201 gacgccatca atacatgcag gttgacggtc agcgcgttca tcgcgacgac ctcgcccgtc
     1261 tcggcgccgg cgagtgccgc gagtcccgcc tgcatcagtg aggcgtagtc gacccagggg
     1321 cgctggccga cgaactgtcc gtcgatgccg cgcgcggccc agctgtcgag ttcctcgttc
     1381 acgatccggc gcgctgcgat cggcatcagg ccgagcgaat ggccgcagaa atagacgagc
     1441 ggttcaccgc tggcatcgcg tggcagctcg aagcgctcgc gcatcacgta acgggtccgt
     1501 ggatcgtctc gtaccggtag gcgagcgggc ggctcggcac cgcatcgccg gccatggccg
     1561 gcacctgcca cgcgagcagg cacgggccgt cggccaggct gtcagggatg tgcgcgagct
     1621 cggtgacggt cgcgtacgcc cgcgccgcat cggcgagccg gcggctcttc ggcggcaggc
     1681 cgaagaatat gcggtgcgcg gcaaggaggc cgccatcgtg cgtgcgatcg agcgatggca
     1741 gatcgagtac caggtgcgca atgccgagcc cgacgatcca gctggcggcg tcggccgcga
     1801 catagggcgc catcgagccg ggcgcggtgg cgccggcgtc gttcgggtct ggcagcgttc
     1861 gcagtatcag tgcctgcggg acgacaggcc ccggaggcac gtcggcgcac tgccatgctg
     1921 cggccagcgc cgcgcgggtg atgacccgat cctcgccgat gcgatccggc acgacactca
     1981 cgacgatcgc ggcaaccggc gcgcgcggga ccacccgcca ggcgtcgagc gcctcgacgg
     2041 tcaggtgcgc cacgcactcg gtgtgagtgc cattgcagtg gggcgtgagc gtgatggttc
     2101 ggcaattgca gctcgcgccg gtcgcgacat cgccgctgaa gtcaccgatc gagaacggtc
     2161 gcgcctcggc cgccggtgcc gcgaagtgtc gcgcgctcgc cccgtcgaag ccgagcggcc
     2221 gcgcgagctc gacggcctgc gcgaggtcga cgcgcagttc gctgctgccg actgcaaagc
     2281 tcgctttcac gaacggcgtg ctgccggcgg ctgctgcacg gtgccgcatc gccggcagct
     2341 gcgatgcgcg aggctcgcgt agtagcgctc gaatacaggc gggaactgcg tctcgatatc
     2401 ggtcagttcg aattcctccc gatagagctc cgcatggcag ttgtcgcaga accaggcgag
     2461 gccgtcgagt tcaccggggc ggcgcacgcg ctcgattacg agtcccaccg tgtccgggaa
     2521 gcgctgcggg ctgtggggca cgtgggcggg cagcagcaac acctcgccct cgcggatcgg
     2581 cacgtcgacg atgcggccgt cctgcaccac cttgagcagc atgtcgcctt ccagctggaa
     2641 gaaaaactcc tcgcccggat cgaggtggaa gtcgttgcgc tggttcggtc cgcccacgac
     2701 catgatgatg aaatcgcctt cgcggaacag tcgccggttg ccgacagggg gcttgaggtc
     2761 gtcgcgatgc gcatcgatcc acgacttcag agagaaagcc ttgagctgtg tcatccgcta
     2821 atggtacaca cgccggcaag aggtgcgagc atggtgcgga acagggcctg gatcggcgtg
     2881 ggactggcgg ctgccatggt agccggctgc ggcggcatgc cacagttgtc ctggccatgg
     2941 gcgagggcgg gcgcgcctgc gccgcaaccg gtcgacgaac tgacattcgc gggcgacgct
     3001 accgcgccga tcgcccagca ctggcagggc aacacgctgg tcctcgattt caccacggtg
     3061 cccgccgaag ggcaggcgag cgccacgccg acccatgcgc gcggctggcc gctgcgcatg
     3121 gcggtgcgca cctggccggg gcgttttggg gccctcgaag tgcgcggtgc gcagcgcgtg
     3181 ctgctgccgc tgacgaccga cggcagcgac atggtggatg tggccatacc gccggaagtg
     3241 ttcgcggctg gcacgccgca gatcgagctg cgctggggcg cagcgatcgc gccgccggtg
     3301 gtgtttccgc aggcgccgcc gccggcaccc gccgcggcga gtgccgcgac cgatcagtcc
     3361 gcgccgatgc gctgataggc gccgcaggcc acctgcagcg gcaggcgctg cggatcgtcg
     3421 agcgacagtg ccgtgagcgc gccaccccat acgcaaccgg tgtcgatggc gatcacgccg
     3481 ttgctgtccg agtacccgag cgccgaccag tggccgcaga tcacccgcgc gccccgcgtg
     3541 cgcgcgctgc cgtaactgaa ccagggtgcg agcggcggcg cgacgtcgcc cggggccgcc
     3601 ttctccgcca ggtccatgcg ccccccgggc gtgcagaacc gcagtcgcgt cagtgcgttg
     3661 atgatgaagc gcaatcgcgc catgccctcg aggttctcgt cccaggtgtc cggctggttg
     3721 ccatacatcc cggcgaatac cgccggtgcg tccgcggcca gcgccgctcc gacctcgcgt
     3781 gcgagccgca cggcgtcgac cgcgcgccac tgcggaacga gtcccgcgtg taccagcagg
     3841 tcgccgcggt ccgcgtcgtg gtgcgcgagc ggttgcgcca gcagccactc gagcagttgc
     3901 tcgcggtcgc tcgcctcgag caccgcgtcg agcgtgtcgc cgcgccggag tctgcggctg
     3961 ccgtgtccga acgccaccgc aagcaggtgc aggtcgtggt tgccgagcac cgtgacggcg
     4021 ttgccgccca ggtcgcgcac gaagcgcagc acctcgagcg atttcggtcc gcgattgacc
     4081 aggtcgccga cgaaccacag ctcgtcgcga tcggaggaaa accgcagcgc gcgcagcagc
     4141 gcgcgcagct cgtcgtagca accctggatg tcgccgatcg cgtagcgcgc catcgcgcct
     4201 agtgcaggac gccggggatc gcgaggcgaa atggcgcgat cggcgcgtcg aaggccagac
     4261 cttcgtcgtc gaccatctgg tagctgccct gcatcgcgcc gaccggtgtt tcgatcatgg
     4321 tgccgctcga atagcggaag ccctggcccg gtttcaggta aggctgctcg ccgagcacgc
     4381 cgtcgccacg cacttcctgc accttgccgt tggcgtcggt gatcagccag tgccgcgcga
     4441 gcagccgcgc cggcgtcgtg ccgtcgttgc gtacggtgat cgtgtaggcg aacacgtacc
     4501 ggtgctcgcc cgggtcggag cgctcgtcga gataaacggt ttcgacgtcg acgcggatgc
     4561 gatgcggcgc gctgctcatc gggccgcgcg gaccgacacg taccgcgagc acgtcgcacg
     4621 gcgcgccgtg cagcacggcg tcttcggtga gattgaccag gatcgacagg ccgtggcgct
     4681 cgcggctgcc gaggacgatc aggtcgacat tgcgctcgcg ggccacgcga atgatctcgg
     4741 ccttcacgtt gcccgcctcg acatgcacgg gggcttcggc gaggcccagc gatccggcga
     4801 gcgcggtgag acgctggcgc gcgcgctgca cgagttcctc ctcgatggcg acggccggca
     4861 tcagggtctc gcccatgggc tcgacgggca cgaattcgac gacgtgcagc agttcgaggt
     4921 cggcgtgaaa ggtttcggcg aggaacttcg cgcgctcggc gaccggcgtg ctgtcctcgg
     4981 tgaggtcaac gggaagcagg atgcgtcggt aggcgtccat accaccaaac tatcgcccct
     5041 gcgcgatgcg tgcaagcgcc ccgaagccct ccggcgcgac ggtctccgcg cgcgcccccg
     5101 gatcgacccc ggcggcctcg atcccggcct cgtcgagcag ttcgcgcagc gcattgcgca
     5161 aggtcttgcg ccgctgcgag aacgcgagtg cgacgacccg cgggaaggcc ggcgcgatgg
     5221 cgaacgcggg cgtttcgcgc accgtgaggc gggcgacggc cgaccagacc ctgggtgggg
     5281 gctggaacgc accaggcccc acgtcgaaca gtctctcgat gcgcacccac ggcgcgagca
     5341 tcacggtgag tcgtccgtac tccttgtggc ccggcgccgc cgccatgcga tcgatcacct
     5401 cccgctgcag catcacgtgc aggtcggcga tcgcagcggg ggtcgcgaga aggtggaaca
     5461 gcagcggcgt cgagatgttg tagggcaggt tgccgacgat gcgcaggcgc gcgccgcgcc
     5521 gggccgcgag tgccgcgaaa tcgaaggcga gtgcatcgcc cacgtgcagc gtgaggcccg
     5581 cctccggcgg aaactcccgc gcgagcccct gggcgagatc gcgatcgatc tcgatcgcat
     5641 cgatgtggcg ggcggcggcg agcagccgcc gcgtcagcac gccgcggccc gggccgatct
     5701 cgacgatggg ctcgccgggt tgcgggtcga tcgcctgcac gatgcgatcg acgatcgccg
     5761 gatcgtgcag gaagtgctgg ccgaagcgct tgcgtgccgg agccggcacg tcaatgccgc
     5821 cggttggcca tctcgatcgc gagcgcgagc gccgcttcga ggctgccggc atcggcgcgg
     5881 cccgtgcccg cgagatcgag cgcggtgccg tggtctaccg aagtgcgaat gaccggcagg
     5941 ccgagtgtga cgttgaccgc acggccgaag ccggcgtgct tgagcaccgg caggccctgg
     6001 tcgtggtaca tcgcaagcac ggcatcgacg ccggcgagtt gcgccggcac gaaggcggtg
     6061 tcggccggca cgggaccctt gacgtcgagg ccgcgcgcac gcgcatccgc gagcgcaggc
     6121 gcaatgacgt cgatttcctc gctgccgaga tgaccgcctt cgcctgcgtg cgggttcagg
     6181 ccgcagacca ggatgcgcgg gtgggcgatg ccgaacaggc gcgtgaggtc ggtggccagc
     6241 acgtcgagca cctcgcgcag cagcgcgggg gtgatcgcgt ggctgaccgc gcgcagtggc
     6301 aggtgagtgg tcgcgagcgc gacacgcagg ccgccggcga ccagcagcat gaccgggcgc
     6361 gtgatgccgc catgcgcggc gaaatactcg gtgtggccgg tgaaggcgat gcccgcgtcg
     6421 ttgatgatgc ccttgtgcac cggcgcagtc acgatcgcat cgaactcgcg ggtcacggca
     6481 ccggccatcg cggcatcgag cagcgacagc acataggagg cattgcgccg ctcgaggtgg
     6541 ccgggctgcg agggtgcgcc gagggggcag tgcgcgacgc gcagctcgcc cggcacgtgt
     6601 gcgcgctgct ggtcgcgcgc atagtcatgc agcacgagcg aattgccggt cgatgcggcg
     6661 cgctcggcga gcagggcgcg atcgccgaag cacaccagtt cgcagggcca ggccgagcgc
     6721 gcgagcgtcg cgcacaactc ggggccgatg cccgccggct cacccgacgt cagggcgatg
     6781 cggggcaggc ggctcacgcg atcacatctt gagttcgacg taggcttcgt cgcgcagccg
     6841 gcgcagccac agttcggttt cttcctcggc cttgctgtcg cgcaacgcca cgtacgcgcg
     6901 ctggcgcttc acttcctcgc tgttgtcgat ctcgcgctgg ccgagccgtt gcacgatgtg
     6961 ccagccgtac tgggtacgga atggctggct gatttcgcca tccgcgagcg ccgcgagctg
     7021 cgcctcgaat tccggcacga acgtgcccgg actggtccag ccgaggtcgc caccctcgga
     7081 tgcggagccc gggtcctcgg acacgatggt ggcgatgccg ccgaaatcct cgccccgcat
     7141 gatgcgatcg cggatcgagt tcatgcgctg ctcgacggtc gcatcgtcct gcagctcgtt
     7201 cggcttcatc aggatgtggc gcgcgtgcac ctgttgcacg atggacgggc cgtccgtgct
     7261 ctgcacttcg ttcacgcgca ccagatggaa gccgctcggc gtgcggatca cgtcgctcac
     7321 ctcgcccgcc tgcagcttga tcaccgcctc ggcgagcacg gttggcagtt cgggaccctg
     7381 gcgccagccg agcgcaccgc cttcgagtgc ggtctggctg ttcgaatagg tgacggcgag
     7441 ctgcgcgaaa tcctcgccgg cgacggcacg ccggtgcacg tcctcggcgc gctgttcggc
     7501 cgccgccatc tgctccggcg tcgcggcctg cggtaccgcg atcaggatgt gcgacaggtt
     7561 gtaggcggtc gattcggacg gcgcgttctt ctggtgctcg aggtactggt cgagttcgcg
     7621 cggcgtgacc acgatccgcg cgatgacgtc gcgctggcgc agcatgctga gcgtgatctc
     7681 cttgcgcagg ctgtcacggt aggcggcgta gtcgacgccc tggctggcaa gcacctgcgg
     7741 cagctgggcg agaggaatgc cgttgcgttc cgccaccccc ttcagcgcgt tgttgaccat
     7801 ttcgtcggtg acattgagcc cggcgcgctt ggcgcgctgc atctgcactt cctggatcac
     7861 gaggcgctcg aggatctgct ggcgcagcac gttctccggc ggcagctcga gtccctgctg
     7921 gcgcaggcgc gcgccgatct gcgcggtctg ctcgtcgagt tcgctcctca gcacgacgcc
     7981 gtcgttgacg atcgccgcga cgcggtcgac gagctcgccg gcggcggaaa gttcgcgcgt
     8041 ctgggcgtgg gaggcgcccg cggccaggat cgcgagcagg ccggcgagaa agggtcgtag
     8101 cattgatttt ccgtgatgtg tcaagggctt gggttggccg gagagtatcc ccgaattgcc
     8161 tcttcgagga aagccaccgc cgatgatccg acactggcca ggccgctgag ttccagttgc
     8221 aggtagatcc cggtgtcacg ctcgccggtg cggctgctga cgaagcggcg gcccactgcg
     8281 cggaagcgcc agcagcacga gcggtattcg aagccggcga actggtcgag ggcgtcgctc
     8341 tcctgcatcg agtagacgta gcgcccgaac aggctccagc ggtccgagac cggccaggcg
     8401 agcgacgcct ccgcctggtc cacgcggtcg cgctggtagc ggtagcccag gttgacgacg
     8461 ctcgtcgagg ctggccggta ctgcagctgc acgtgcatgc gctcgcgctc ggaagtctcc
     8521 ggattccact gtacgccgac atcggcgttc cagttctgcc atgcggtgat cgccagctgc
     8581 gcgatcacgt ccgacgtatc gcggtcgacg atgacttcgc cgggcagggc aacgcggggc
     8641 gcatcgaagt acacggtctg gccaaccgtc gccgcgagca gctggcgacc gctggccgag
     8701 tcgaggatgc gactcgtgat gccgaaactc gcctggtcgg cgtcgctcac gcggtcgcca
     8761 ccgacgtagc ggttggtgcg aaacagctga accaggttca ggtcgggcac cgccgtgtcg
     8821 aacactggca ggtcgtcctg gttgcggtag ggcgtataca cgtacagcgc gcgcggctcg
     8881 agcgtgacgc gtcgctggtt gcgcgagccc gcggcgccct cgaagatcag cccggcatcg
     8941 aggctgacgg tcggcagatt gcgccgcggc gagtcgtcga tgcccggcgc cgtatcctcg
     9001 agcgaatagc cggtcgagcg ccagctcacg gcgggtcgca ggaaataacc ggcgccctgc
     9061 cagtcgaggc cgatgtgtgg tgcggcgtcg tagcgccatc cggtgacgcc ggtgttgcgg
     9121 tcgaacttga tgacctcggc gtcgaaacca tagcgcagtt gcaacgactg cccgattcgg
     9181 aagtcggcgc tcgcgtacag ttgcggcagc atcttgtacg ggcgatcgtc ggccgcaacg
     9241 tcggtgtcga tcgtctggaa gtcctggaat gcgccgcgca gccgccagtt cgcgtcgcga
     9301 taggtgagct ccgcgccgcg ttcgacgaag gtgatgctgg tgccctccgg gccctggccg
     9361 aagtcctcga agtactgcgt gtcgctcgca ttcgccgcat cgatggtgaa gcgccagccg
     9421 cgcggcagtt cggtgcggtg aacgagcttc accaggctgc ggtcgccgtc gaacagttgg
     9481 tcgtccggca ggtaggacac gtcgaggctg ccgcgactgc ggcgtgtcag atagcggaag
     9541 tcgcccgaga ggtcgacgcc gcggcgtgcg aaatagaccg gctcgaaggt gaaatcgcgg
     9601 ttcggtgcga tgttccagta ataggggacg ctcaactgcg cgccgctgcg tgaggaatag
     9661 cccgcgcccg ggaacaggaa gccgctcttg cggtccgagc cgagcgggaa cgagatccag
     9721 ggcgtataga agatcggcac gcccttgaat tcgacgcgtg tgccgcggcc cacgccgttc
     9781 ctcgcggccg tgtcgagatc gatgcggtcg gcgcgcagct gccaggccgg atcggtgagc
     9841 gggcaggtgg tgaaggtgac gcgctcgaga ccgatatgcc cgcccgtatc gagcgtcatc
     9901 gcgccagccg tcccgcgcgc gggccgcgac ggcaacagga actccgcacc gctgaaggtc
     9961 gcgccttcgg tggccgagta ctggcccgag ctgccgcgca ctgtcagcag cggatcgcgg
    10021 tattcgacgt tgccatcgac cgacagtttg ccagcgtccg cgtcatacgc gacgctgtcc
    10081 gccgtgatcc gcctgtcgcc ctgtgtcacg aggacattgc ccgccagcac ggcgtcgccg
    10141 tcgacgccga ggcttgcgtc gtcgctcgac acctcgatcg gttcgttcct gagctcttcg
    10201 gcgctgcgcg gaaccgggct cgcgccctgg ggcagaccac cgctgtcggc cagcgcgccg
    10261 aagcacagtg ccggctcggg atcggccgca tgcgctgcgg cctgcagcaa cagggtgccg
    10321 atcacggcag cgccagacca gtgaacgcgg ggggaaggca tgtggcgtgc cattataatg
    10381 agacaatgac cgaaaacgac gcgcgcgcgg cccaattgca ggaatggctc accgcgggac
    10441 tcggccgccc gatcgagagg ctgacgctcg cgtcggccga cgcgagcttt cgccgctact
    10501 tccgcgtgca cgccgacggc gtcagctgga tcgcgatgga tgccccgccc gggcgcgagg
    10561 atgtgcgccc ctacctgcgg gtggcgcggt tgctcgaaga aaccggcgtg cacgttccac
    10621 acgtgcatgc attcgacatc gagcagggct tcgtattgct cgaggacctc ggtgatacgc
    10681 cctatctcac gcagctcgcg cgtggcgagg atgtcgagcg gctgtacgcg gacgcgctcg
    10741 cggcgctcgc ggccatccag gtgcgtggcc gcgaggccgc gcgtgaactg ccgccgtatg
    10801 acgaccagac actgcagcgc gaactcgacc tgatgccgga atggttctgc gcgcgccacc
    10861 tgcgccttgc gctcgacgac ggcgaactcg ggctcatcgt gcgcaccttc gacttcctga
    10921 tcgaggaggc actcgcgcag ccgcgcgtgt tcgtgcatcg cgactaccac tcgcgcaacc
    10981 tgatga
//
LOCUS       DAOQVY010000076        10767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_6879_length_10767_cov_6.882001, whole genome shotgun sequence.
ACCESSION   DAOQVY010000076 DAOQVY010000000
VERSION     DAOQVY010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10767
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6879_length_10767_cov_6.882001"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PKN91_08135"
     CDS             complement(<1..601)
                     /locus_tag="PKN91_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010117196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNS28079.1"
                     /translation="MATGKITQIIGAVIDVEFPRESIPRVYEALKLKDADLTLEVQQQ
                     LGDGVVRTIAMGASEGLKRGLPVETTGEPISVPVGKATLGRIMDVLGAPQDNRGPVSA
                     KEHWSIHRPAPSYDEQAGGSDLLVTGIKVIDLLVPFAKGGKVGLFGGAGVGKTVNMLE
                     LINNIAKQYSGLSVFAGVGERTREGNDFYHEMAESGVIDL"
     gene            complement(619..1479)
                     /gene="atpG"
                     /locus_tag="PKN91_08140"
     CDS             complement(619..1479)
                     /gene="atpG"
                     /locus_tag="PKN91_08140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNS28080.1"
                     /translation="MAGTKEIRTKIVSVKSTQKITKAMQMVATSKMRRAQERMRLARP
                     YAEKMVTVIGHLSAANPDYRHPFLVTREPKSIGIIVISTDRGLCGGLNANIFKNTIYL
                     MREWQQKGAEVNLCLIGSKGLGFFRRLGLPILANITHLGDRPHVKDLIGTVKVMLDAY
                     REGRIDRLFMVNAQFVNTMTQKPVVEQLLPVAPVESSDLAEHWDYIYEPSAAGILDGV
                     LMRYIESQVYRGAVENVASEMAARMVAMKAASDNAGKLINELQLIYNKARQAAITKEL
                     AEIVGGAAAV"
     gene            complement(1489..3027)
                     /gene="atpA"
                     /locus_tag="PKN91_08145"
     CDS             complement(1489..3027)
                     /gene="atpA"
                     /locus_tag="PKN91_08145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNS28081.1"
                     /translation="MSVKASEISDLIKQRIEKFSSATEARNVGTVVSITDGITRIHGL
                     ADVRYGEMIEYPGGTFGLALNLEQDSVGAVVLGDYTDIREGDTVKTTGRILEVPVGPE
                     LLGRVVDTLGNPIDGKGPLKATRTAPIERVAPGVIYRKSVSQPVQTGYKAVDSMVPIG
                     RGQRELIIGDRQTGKTALAVDTIINQKSSGIKCIYVAIGQKQSSIANVVRKLEEHGAM
                     AHTVVVAASASVPAALQYLAAYSGVTMGEYFMDNGQDALIIYDDLSKQAVAYRQISLL
                     LKRPPGREAYPGDVFYLHSRLLERSARVNEEYVEQVTNGAVKGRTGSLTGLPIIETQA
                     GDVTAFVPTNVISITDGQIFLETDLFNAGIRPAMNAGISVSRVGGAAQTDIIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRRQLERGERVTELMKQKQYAPMSVAEMALSIYA
                     VNNGYMDKVDRRKVVDFEAALQSFAKGSYQDSMDAINAKPELKAHEATLKKICEDFLA
                     TGAY"
     gene            complement(3047..3583)
                     /locus_tag="PKN91_08150"
     CDS             complement(3047..3583)
                     /locus_tag="PKN91_08150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNS28082.1"
                     /translation="MAEIATIARPYARAAFEYAQDAKALARWGDLLDIAAAVVAEPGF
                     SALIGNPKVADDALADLVLEIAGDKADPHGRNYVQLLAQNRRLTLLPEIARQYALLRA
                     DAENTVDVEITSAMPLTPDQTETFARALTRRLNRVVRLHTHVDPSLIGGAIVRTGDLV
                     LDGSLQGRLQRLSQTMTN"
     gene            complement(3576..4055)
                     /locus_tag="PKN91_08155"
     CDS             complement(3576..4055)
                     /locus_tag="PKN91_08155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004411.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNS28083.1"
                     /translation="MTVNATFIGQMVVFAILLWFFWKFVVPPLARANTARQKKIAEGL
                     AAAERGQKDLEEAKSRADAVVREARERANQVLDQANRRSNEMIDKAKQSAGAEGDRLL
                     ANARAEAASEAARARDALRAEVGALAVAGASRLLGREIDAKAHQKLLDELAEEIVRG"
     gene            complement(4078..4347)
                     /gene="atpE"
                     /locus_tag="PKN91_08160"
     CDS             complement(4078..4347)
                     /gene="atpE"
                     /locus_tag="PKN91_08160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HNS28084.1"
                     /translation="MEAIATVQAYTALGIGIIIGLGAIGACIGIGVMGSKFLESAARQ
                     PELTPMLQGRMFLLVGLIDAAFLIGVGLAMLFAFANPLLAQLSGG"
     gene            complement(4393..5220)
                     /gene="atpB"
                     /locus_tag="PKN91_08165"
     CDS             complement(4393..5220)
                     /gene="atpB"
                     /locus_tag="PKN91_08165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNS28085.1"
                     /translation="MASASGAEHGTSPTDYIVHHLTHLHVGEGFWTWHIDTLFMSALL
                     AIVVGWIFWAAARRATSGVPGKFLGFIELIYEFVDGQVADIYHGDRSFMVALALSLFT
                     WIIAMNTMDLLPLDFPGGVAALFGLEYWRVLPTADLNGTIAMAIVVLLLIIIAAIRAK
                     GLGGYLHEWVSAPFGSNPLLWIPNVCLNLVELLSKPVSLGMRLFGNMFAGELLFMLIA
                     LLGFTVVGVTFGSALGFFGQVVMGTAWAIFHILIVVLQAYIFMVLPIVYIAMAEEHH"
     gene            complement(5243..5641)
                     /locus_tag="PKN91_08170"
     CDS             complement(5243..5641)
                     /locus_tag="PKN91_08170"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HNS28086.1"
                     /translation="MIQSREPDIRRVAAWILVVQVAVTLAAAALCLAAWGSRHGGSAL
                     AGGSIGVIANLYMTLATLRRGGGAVLVLSRFLIGQFVKVGLTVAMFIIVARTGRAVWP
                     PLIATYVATLVVFWAVPAIAAPRLPPRSRG"
     gene            complement(5788..6699)
                     /locus_tag="PKN91_08175"
     CDS             complement(5788..6699)
                     /locus_tag="PKN91_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNS28087.1"
                     /translation="MTQKKTTLGRGLADLLGQSAARAASAPSAARAAEELARLPLDLL
                     QRGRYQPRVDMRPESLQELADSIKAQGVVQPIVVRPLVGAGEGATQRYEIIAGERRWR
                     AAQIAGLADIPAVVRHVPDESAIAMALIENIQRENLNPLEEARALERLIAEFGVTHQQ
                     AADAVGRSRAAVSNLLRLLELAPEVSELVEKRSLEMGHARALLGLGQRRQQAEVAALV
                     ARKGLSVRETEALVRRLTAPATEAGGAEEAGTGRRQDPNTAQLEGQLAERLGAKVSIQ
                     HGAGGKGKLIVSYNSLDELDGILAHIS"
     gene            complement(6696..7499)
                     /locus_tag="PKN91_08180"
     CDS             complement(6696..7499)
                     /locus_tag="PKN91_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS28088.1"
                     /translation="MKRVIAVANQKGGVGKTTTAVNLAASLAAMKRRVLLIDLDPQGN
                     ATMGCGVEKSQVARGTCEVMLGECDIESAIVPIEQAGMFLLPANQDLTSVEVRLLTMM
                     TGREMKLRNALRPVRERFDQIIIDCPPALNMLTVNALVAADSVLVPMQCEYYALEGLS
                     ALVSTIEQIRAAVNPDLEIEGILRTMFDPRNNLANEVSAQLIEHFGDKVFRTVVPRNV
                     RLAEAPSFGRPAMFHDKESRGALAYLALAGEMLRREEESRPPERLSSVS"
     gene            complement(7510..8478)
                     /locus_tag="PKN91_08185"
     CDS             complement(7510..8478)
                     /locus_tag="PKN91_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28089.1"
                     /translation="MRTRQPALAVLVMLGFACVCSVAQARDRIEARIDDFVLVTDANA
                     QSWLTAHYMLIGNPQRSRQTGAYLQALTDGRPIAEAVQAAYGLSIDELDREIRGYRNR
                     GRIAGYRLRFNEPLPDANDVVIRPLPEAVALSRLAAAGIAVGRTSPDRIAERVERALQ
                     QKEPAADDDKMKTEAEVESEGELMDEAEDEGVVEPSISTGDERTQLSPEQSAMLQAAR
                     TAVLPFAGDPDSGISATLTVAGIDALLSDRKLEDTLALVQQSVAIYPSHAELALAEAG
                     LCQKLGRNVAALASASRAARYTRSPELRRRIESWVQELEAEADSSR"
     gene            complement(8486..9133)
                     /gene="rsmG"
                     /locus_tag="PKN91_08190"
     CDS             complement(8486..9133)
                     /gene="rsmG"
                     /locus_tag="PKN91_08190"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNS28090.1"
                     /translation="MSAAERSAEAARLAAGASALGAPLDPAQASTLLRLLDELERWNR
                     SFNLTAIRDRGGMLTHHLLDSLSIRPFLAGGTVADVGTGAGFPGLPLAIVEPARRFTL
                     IDSNNKKIRFIAHAARTLGLGNVEPLHARVEDLAPAQPFDTVVARAFAPLPRMLDSIG
                     PICGPLTRVLAMKSAQLDEELREVAAPWRVAAVHDLTVPGIDAVRALAILYRDGV"
     gene            complement(9138..10583)
                     /locus_tag="PKN91_08195"
     CDS             complement(9138..10583)
                     /locus_tag="PKN91_08195"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF006943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HNS28091.1"
                     /translation="MGAAISNVRPKPDKVLVDIVDYVSKDKITGKLAYETARNCLIDT
                     LGCGLEALEYPACTKLLGPIVPGTVVPNGAKVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILAVADYLSRLRVMEGGKPLTVRDVLTGMIKAHEIQGVLAL
                     ENSFNRVGLDHVLLVRVASTAVVTAMLGGTREQVVNAVSNAWIDGGALRTYRHAPNTG
                     SRKSWAAGDATSRAVRHAFIALTGEMGYPSALSAKTWGFQDVLFKGKPVTLAQPLGSY
                     VMENVLFKISFPAEFHAQTAVECAMQLHPQVKDRIDAIEKIVIETQEPGVRIIDKTGP
                     LANPADRDHCIQYMVAIPLLFGRLTAADYEDAVASDPRVDALRAKMIVQENETFTKEY
                     YERDKRYIGNAVQVFFRDGSSTPRIAIDFPIGHRKRREEGMPVLVRKFESSVAAHFSA
                     KQSAAITRLFADAARLDAMPVHDFVAAMVKN"
BASE COUNT         1692 a         3598 c         3584 g         1893 t
ORIGIN      
        1 cgaggtcgat gacgcccgac tccgccatct cgtggtagaa gtcgttgccc tcgcgagtgc
       61 gctcgccgac gccggcgaac accgacaggc cgctgtactg cttggcgatg ttgttgatca
      121 gctccagcat gttgacggtc ttgccgacac cggcgccgcc gaacaggccg accttgccgc
      181 ccttcgcgaa cggcaccagc aggtcgatga ccttgatgcc ggtgaccagc agatcgctgc
      241 cgcccgcctg ctcgtcgtac gacggcgcgg ggcggtggat cgaccagtgc tccttggcgc
      301 tcaccgggcc gcggttgtcc tgcggcgcgc cgagcacgtc catgatgcga ccgagcgtgg
      361 ccttgccgac cggcaccgag atcggctcgc cggtggtctc gaccggcagt ccgcgcttca
      421 ggccctcgga ggcgcccatc gcgatcgtgc gcacgacgcc gtcaccgagc tgctgctgca
      481 cctcgagagt gaggtccgca tccttcagct tcagcgcctc gtaaacgcgc gggatcgact
      541 cgcgtgggaa ctccacgtcg atgaccgctc cgatgatctg cgtaatcttt ccggtagcca
      601 tttcgcttcc tcaattcctc aaactgctgc ggcgccgccc acgatctccg cgagttcctt
      661 ggtgatcgcg gcctggcgtg ccttgttgta gatcagctgc agctcgttga tcagcttgcc
      721 agcgttgtcc gaggccgcct tcatcgcgac catgcgcgct gccatttccg aggcgacgtt
      781 ttcgacggcg ccgcgataga cctgcgactc gatgtagcgc atcagcacgc catcgaggat
      841 gcccgcggcg ctcggctcgt agatgtaatc ccagtgctcg gcgaggtcgg acgattcgac
      901 cggcgcgacc ggcagcagct gctcgacgac cggcttctgg gtcatcgtgt tgacgaactg
      961 cgcgttcacc atgaacaggc gatcgatcct gccctcgcga taggcgtcga gcatgacctt
     1021 cacggtgccg atcaggtcct tcacatgcgg gcgatcgccg agatgcgtga tgttcgcgag
     1081 gatcggcagg ccgaggcggc gaaagaagcc gaggcccttc gagccgatca ggcacaggtt
     1141 cacttccgcg cccttctgct gccactcgcg catcaggtag atcgtgttct tgaagatgtt
     1201 ggcgttcagg ccaccgcaca ggccgcggtc ggtcgagatc acgatgatgc cgatcgactt
     1261 cggctcgcgc gtcacgagga acgggtgccg gtagtcgggg ttcgccgcgc tcagatggcc
     1321 gatcacggtg accatcttct ccgcgtacgg gcgcgcgagg cgcatgcgtt cctgcgcgcg
     1381 gcgcatcttg ctcgtcgcga ccatctgcat cgccttggtg atcttctgcg tgctcttgac
     1441 gctgacgatc ttggtgcgga tttccttggt gccggccatt gcgcttgctc agtaagctcc
     1501 ggttgccagg aagtcctcgc agatcttctt cagggtcgcc tcgtgcgcct tcagctcggg
     1561 cttcgcgttg atcgcgtcca tcgagtcctg gtagctgccc ttggcgaacg actgcagcgc
     1621 cgcctcgaaa tcgacgacct tgcggcggtc caccttgtcc atgtagccgt tgttgaccgc
     1681 atagatcgac agcgccattt cggcgaccga catcggcgcg tactgcttct gcttcatcag
     1741 ctcggtcacg cgctcgccgc gttcgagctg gcggcgcgtg gcttcgtcga ggtcggaggc
     1801 gaactgcgag aacgccgcga gctcgcgata ctgcgcgagc gcgaggcgga tgccgccgcc
     1861 gagcttcttg atgatgtcgg tctgggccgc gccaccgacg cgcgacaccg agatgccggc
     1921 gttcatcgcc gggcggatgc ccgcgttgaa gaggtcggtc tcgaggaaga tctggccatc
     1981 cgtgatcgag atcacgttgg tcggcacgaa cgcagtcacg tcgcccgcct gcgtctcgat
     2041 gatcggcagg ccggtcagcg agccggtcct gcccttgacc gcgccattgg tcacctgctc
     2101 gacatactcc tcgttgacgc gcgcgcttct ctcgagcagg cgcgagtgca ggtagaacac
     2161 gtcgccggga taggcttcgc ggcccggcgg gcgcttcagc agcagcgaga tctggcgata
     2221 ggcaaccgcc tgcttggaca ggtcgtcata gatgatcagc gcgtcctggc cgttgtccat
     2281 gaagtactcg cccatcgtga cgcccgaata ggcggcgagg tactgcagcg cggccggcac
     2341 cgaggcggag gccgcgacga ccaccgtgtg cgccatcgcg ccgtgctcct cgagcttgcg
     2401 tacgacgttc gcaatcgacg actgcttctg gccgatcgcg acgtagatgc atttgatgcc
     2461 cgaggacttc tggttgatga tcgtgtcgac ggcgagcgcg gtcttgccgg tctggcggtc
     2521 gccgatgatc agctcacgct ggccgcggcc gatcggcacc atcgagtcga cggccttgta
     2581 gccggtctgc accggctgcg acacgctctt cctgtagatg acgcccggcg cgacacgctc
     2641 gatcggcgcc gtgcgggtcg ccttcagcgg gcccttgccg tcgatcgggt tgccgagcgt
     2701 gtcgacgacg cgaccgagca gttcgggacc gaccggcacc tcgaggatgc gtccggtggt
     2761 cttcaccgtg tcgccctcgc ggatgtcggt gtagtcgccg agcaccactg caccgacgga
     2821 gtcctgctcg aggttgagcg caagcccgaa ggttccgccc gggtactcga tcatttcgcc
     2881 gtagcgcaca tcggccaagc cgtggatgcg cgtgatgccg tcggtgatgg acaccacggt
     2941 gccgacattg cgcgcctcgg tcgcggacga gaacttctcg atccgctgct tgatcaggtc
     3001 gctgatttcc gatgctttga ctgacatggg atggggtcct tgggaatcaa ttcgtcatgg
     3061 tctggctgag tcgctggagc ctgccctgga gcgaaccgtc gagcaccagg tcgccggtgc
     3121 gcacgatggc gccgccgatg agcgacgggt cgacgtgggt gtgcaggcgc acgacgcgat
     3181 tcaggcggcg cgtgagcgcg cgcgcgaacg tttcggtctg atccggcgtg agcggcatcg
     3241 ccgaggtgat ctcgacatcg accgtgttct cggcatccgc acgcagcagc gcgtactgcc
     3301 gcgcgatctc cggcagcagc gtcaggcgcc ggttctgcgc gagcagctgc acgtagttgc
     3361 ggccgtgcgg gtccgccttg tcaccggcga tctcgagcac gaggtcggcg agcgcgtcat
     3421 cggccacctt cggattgccg atcagcgcgc tgaagcccgg ctccgcgacg actgcggcgg
     3481 cgatgtcgag caggtcgccc cagcgcgcga gcgccttcgc gtcctgcgcg tactcgaacg
     3541 ccgcgcgcgc atagggtcgc gcgatggtgg cgatttcagc cacgcacgat ctcctcggcg
     3601 agttcgtcga gcagcttctg gtgcgccttc gcgtcgatct cgcggcctag caggcgcgag
     3661 gcgcccgcga cggccagcgc accgacttcg gcgcgcaacg cgtcgcgcgc ccgcgcggcc
     3721 tcgctggcgg cctcggcgcg cgcattcgcg agcaggcggt cgccttcggc gccggccgac
     3781 tgcttggcct tgtcgatcat ctcgttcgag cggcggttcg cctggtcgag cacctggttt
     3841 gcgcgctcac gcgcctcgcg caccacggcg tcggcacgtg acttcgcttc ctcgagatcc
     3901 ttctggccac gctcggcggc cgcgaggccc tcggcgatct tcttctggcg cgcggtgttc
     3961 gcccgggcaa ggggcggaac gacgaacttc cagaagaacc agagcaggat cgcgaatacg
     4021 accatctgcc cgatgaatgt cgcgttgacg gtcatcgccc gtctccttca gcgtggatca
     4081 accgcctgag agctgggcga gcagcgggtt cgcgaacgcg aacagcatcg cgagaccgac
     4141 accgatcagg aacgccgcgt cgatgaggcc cacgagcagg aacatgcggc cctgcagcat
     4201 cggggtcagc tcaggctggc gcgcggcgga ttcgaggaac ttcgaaccca tcacgccgat
     4261 gccgatgcac gcgccgatcg cgccgaggcc gatgatgatg ccgattccaa gcgcggtgta
     4321 cgcctgaacc gttgcgattg cttccatctg agttctcctg gtcgattcga ataaaaagaa
     4381 gctgaacgaa acctagtgat gctcttcggc cattgcgatg tacacgatcg gcaacaccat
     4441 gaagatgtag gcctgcagga ccactatcaa gatgtggaag atcgcccagg cggtgcccat
     4501 gacgacctgg ccgaagaagc cgagtgcgct gccgaaggtg acgccgacca cggtgaagcc
     4561 gagcagcgcg atcagcatga agagcagctc gccggcgaac atgttgccga acagtcgcat
     4621 gccgagcgac accggcttcg acagcagttc gacgaggttc aggcagacgt tcgggatcca
     4681 cagcagcgga ttgctgccga acggcgccga cacccactcg tgcaggtagc cgccgaggcc
     4741 cttggcacgg attgccgcga tgatgatcag cagcagcacg acgatcgcca tggcgatcgt
     4801 gccgttcaga tcggcggtcg gcagcacgcg ccagtattcg aggccgaaca gcgccgcgac
     4861 cccgcccggg aagtcgagcg gcagcaggtc catcgtgttc atcgcgatga tccacgtgaa
     4921 cagcgacagc gcgagcgcga ccatgaagct gcggtcaccg tgatagatgt cggccacctg
     4981 gccgtcgacg aattcgtaga tcagttcgat gaaaccgagg aacttgcccg gtacgcccga
     5041 ggtggcgcgc cgcgcggcgg cccagaaaat ccagccgacc acgatggcga gcagcgccga
     5101 catgaacagc gtgtcgatgt gccaggtcca gaagccctcg ccgacatgca gatgggtgag
     5161 gtgatggacg atgtagtcgg tcggcgatgt gccgtgttct gcgccgcttg cggatgccat
     5221 ctgttcgagc ctttaccctg cctcaccctc tcgaccgcgg cggcagccgg ggtgccgcaa
     5281 tcgcgggcac cgcccagaac accaccagcg ttgcgacata ggtcgcgatc aacggcggcc
     5341 acaccgccct gccggtccgc gcgacgatga tgaacatcgc cacggtcagc ccgaccttca
     5401 cgaactggcc gatcaggaac cggctgagta cgagaaccgc gccgccgcct cgtcgcagcg
     5461 ttgccagggt catgtacaag ttcgcaatga caccaatcga gccgccggcg agcgccgatc
     5521 cgccgtgccg cgacccccac gccgccaaac acagcgcggc cgccgccagc gtcacggcca
     5581 cctgcacgac caggatccat gcagcgactc gcctgatgtc aggttcgcgc gactggatca
     5641 cgttcggacg ggaggcggcc ttcagcccaa ttttccttgc acggcagcca cttagggccc
     5701 cgtttgcgac gcgcgcggat tatagggggg aagcccctcg ccagcaagaa atacggggga
     5761 aatacttagc agactcggtg ccgagtctca gctgatgtgc gccaggatgc cgtcgagttc
     5821 gtcgagactg ttgtagctga cgatgagctt gcccttgccg ccggcgccgt gctggatcga
     5881 gaccttcgcg ccaagcctct ccgccaactg gccctcgagc tgcgcggtgt tcggatcctg
     5941 ccggcggccc gtcccagctt cctcggcgcc gccggcctct gttgccggcg cggtgaggcg
     6001 gcgcaccagc gcttccgtct cgcgcacgga caggcccttg cgtgcgacca gcgccgcgac
     6061 ttcggcctgc tggcggcgct ggccgaggcc gagcagcgct cgcgcatggc ccatctcgag
     6121 cgagcgcttc tccaccagtt cgctgacctc gggcgcgagc tcgagcaggc gcagcaggtt
     6181 gctgactgcc gcgcgcgatc ggccgacggc gtcggcggcc tgctggtgcg tgaccccgaa
     6241 ctcggcgatc aggcgctcca gggcgcgggc ttcctcgagc ggattcaggt tctcgcgctg
     6301 gatgttctcg atgagcgcca tcgcgatggc cgactcatcg ggcacatggc gcacgaccgc
     6361 gggaatgtcg gcgagaccgg cgatctgcgc cgcgcgccag cgacgctcgc ctgcgatgat
     6421 ctcgtagcgc tgcgtcgcgc cttcgccggc gccgaccagc gggcgcacga cgatcggctg
     6481 cacgacgccc tgtgccttga tcgaatcggc cagttcctgc agcgactcgg ggcgcatgtc
     6541 gacgcgcggc tgatagcggc cccgctgcag caggtcgagt ggaagccggg caagttcctc
     6601 agcggcgcgc gccgccgacg gcgcgctggc cgcgcgcgcg gccgactggc cgagcaggtc
     6661 ggcgaggccg cgaccgagcg tggtcttctt ctgcgtcatg atacggatga tagccgctcc
     6721 ggcgggcgcg actcttcttc acgacgcagc atctcgccgg cgagcgccag gtaggcgagc
     6781 gcgccccgcg actccttgtc gtggaacatc gccgggcgcc cgaacgaggg cgcttcggcg
     6841 agccgcacgt tgcgcggcac gacggtgcgg aacaccttgt cgccgaaatg ctcgatcaac
     6901 tgcgcgctca cttcgttggc gagattgttg cgcgggtcga acatcgtgcg caggatgccc
     6961 tcgatttcga ggtcgggatt caccgccgcg cggatctgct cgatcgtcga gacgagcgcc
     7021 gaaagaccct cgagcgcgta gtactcgcac tgcatcggca ccagcacgct gtcggcggcg
     7081 acgagcgcat tcacggtgag catgttgagc gccggcgggc agtcgatgat gatctggtcg
     7141 aagcgttcgc gcaccgggcg cagcgcattg cgcagcttca tttcccgccc ggtcatcatc
     7201 gtcagcaggc gcacctcgac cgaggtgagg tcctggttcg cgggcagcag gaacattccg
     7261 gcctgctcaa tcggcacgat cgcgctctcg atgtcacact cgccgagcat cacctcgcag
     7321 gtgccgcgcg cgacctgcga cttctcgacg ccacagccca tggtggcgtt gccctgagga
     7381 tcgaggtcga tcagcagcac gcggcgcttc atcgccgcga gcgacgccgc caggttgacc
     7441 gccgtcgtgg tcttgccgac accgcccttc tggtttgcga ctgcgatgac tcgcttcatc
     7501 cgagcgagtt tagcggctgg aatcagcctc tgcttcaagt tcctgcaccc agctctcgat
     7561 ccggcggcgc agctccggcg aacgggtgta tcgcgcggcg cggctcgccg aggcgagcgc
     7621 cgcgacgttg cggccgagct tctggcaaag gccggcctcc gccagggcaa gctcggcatg
     7681 gctggggtag atggccacgg actgctggac gagcgccagt gtgtcctcga gcttgcgatc
     7741 cgagagcagc gcgtcgatac ccgcgacggt cagtgtcgcg ctgatgcccg agtccgggtc
     7801 gccggcgaac ggcagcaccg ccgtgcgcgc agcctgcagc atcgcggact gctccggcga
     7861 gagctgcgtg cgctcatcgc ccgtggaaat cgagggttcg accacgccct cgtcttccgc
     7921 ctcgtccatc agctcgcctt ccgactcgac ttcggcctcg gtcttcatct tgtcgtcatc
     7981 ggccgcgggc tccttctgct gcaatgcgcg ctcgacgcgc tctgcaatcc tgtccggcga
     8041 ggtccttccg acggcgatgc ccgcggccgc gagtcgcgac agcgcgacgg cctcgggcag
     8101 cggcctgatc accacatcgt tcgcgtcggg gagcggctcg ttgaagcgca ggcggtagcc
     8161 ggcgatccgg cctcgattgc ggtagccccg aatctcgcgg tcgagctcgt cgatgctgag
     8221 gccgtaggcc gcctgtaccg cctccgcaat gggcctgccg tcggtcagcg cctgcagata
     8281 cgcgcccgtc tgccggctgc gctgcggatt tccgatcaac atgtagtgtg cggtgagcca
     8341 ggactgcgcg ttggcgtcgg tgaccagcac gaagtcgtcg atgcgcgcct cgatccggtc
     8401 gcgcgcctgc gcgaccgagc agacacaggc gaaccccagc atgaccagta cggcaagagc
     8461 cggttgtctg gttctcatgc tcgattcata ctccatcgcg atacagaatg gcaagcgcac
     8521 gcacggcgtc gatcccgggt acggtcagat cgtgcaccgc cgccacgcgc cacggcgctg
     8581 cgacttcgcg cagctcctcg tcgagttgtg cgcttttcat ggcgagtacc cgggtcagtg
     8641 gcccgcagat cggcccgatg gaatccagca tgcgcggcag cggcgcgaac gcgcgcgcca
     8701 ccacggtatc gaacggctgc gcgggtgcga ggtcttccac ccgcgcatgc agcggctcga
     8761 cgttgccgag accgagggtg cgcgcggcgt gcgcgatgaa gcggatcttt ttgttgttcg
     8821 agtcgatcag cgtgaatcgt cgcgccggct cgacgatcgc gagcggcagg cccggaaagc
     8881 cggcgccggt cccgacatcg gcgaccgtgc cgcccgcgag gaaaggcctg atcgagaggc
     8941 tgtcgagcag gtgatgcgtg agcattccgc cgcgatccct gatcgcggtg agattgaaac
     9001 tgcggttcca gcgctcgagt tcgtcgagca ggcgcagcag cgtagaggcc tgggccgggt
     9061 cgagcggcgc cccgagcgca ctcgcgccgg ccgcgagccg cgccgcctcc gcgctgcgct
     9121 ccgcggcgct cacatcgtca gttcttgacc atggccgcga cgaagtcgtg taccggcatc
     9181 gcatcgagcc tcgccgcatc ggcgaacagc cgggtgatcg cggcggactg cttggcggag
     9241 aaatgggccg ccacggacga ctcgaacttc ctgacgagca ccggcatgcc ctcctcgcgg
     9301 cgcttgcgat ggccgatcgg gaagtcgatc gcgatgcgcg gtgtgctgct gccatcgcgg
     9361 aagaacacct gcacggcatt gccgatgtag cgcttgtcgc gctcgtagta ttccttcgtg
     9421 aaggtctcgt tctcctgcac gatcatcttc gcacgcagcg catcgacacg cgggtcgctg
     9481 gcgaccgcgt cctcgtaatc cgccgcggtg aggcgcccga acagcagcgg gatcgccacc
     9541 atgtactgga tgcagtggtc gcgatcggcc ggattggcga gcggaccggt cttgtcgatg
     9601 atgcgcacgc cgggctcctg cgtctcgatg acgatcttct cgatcgcatc gatccggtcc
     9661 ttcacctgcg gatgcagctg catcgcgcac tcgaccgcgg tctgcgcgtg gaattccgcc
     9721 gggaagctga tcttgaacag cacgttctcc atcacgtagc tgccgagcgg ctgcgcgagc
     9781 gtgaccggct tgcccttgaa cagcacgtcc tggaaacccc aggtcttcgc cgacagcgcc
     9841 gacgggtagc ccatctcgcc ggtgagcgcg atgaaagcgt ggcgtaccgc gcggctggtc
     9901 gcgtctcccg ccgcccagct cttgcgcgag ccggtgttcg gtgcgtgccg ataagtgcgc
     9961 agcgcgccgc cgtcgatcca cgcgttcgac accgcgttca ccacctgctc gcgcgtgccg
    10021 ccgagcatcg cggtgacgac ggcggtggag gcgactcgca ccagcagcac gtgatcgagg
    10081 cccacacgat tgaagctgtt ctcgagtgcg aggacgccct ggatttcgtg cgccttgatc
    10141 atcccggtga gcacgtcgcg cacggtgagt ggcttgccac cctccatgac gcgcagccgc
    10201 gagaggtaat ccgccaccgc caggatgccg cccaggttgt cggacggatg gccccattcg
    10261 gccgcgagcc aggtgtcgtt gaagtcgagc cagcggatca tggcgccgat gttgaaggcc
    10321 gcctgcaccg ggtcgagctg gaactgggta ccgggcacct tggcgccatt ggggacgacc
    10381 gtgccgggaa cgatcggccc cagcagcttg gtgcaggccg ggtactcgag agcctcgagg
    10441 ccgcagccga gcgtgtcgat caggcagttg cgggcggtct cgtaggccag cttgccggtg
    10501 atcttgtcct tcgagacgta gtcgacgata tcgaccagca ccttgtcggg cttgggtcgg
    10561 acgttggaga ttgctgcacc catgatggat tcgatcctgt cggtttcggg gattggcttg
    10621 cctgaccggt cctacttgcg cttggcaagc gggacgaact tgcggttctc cgggccgaca
    10681 tagttggcgc tcggacggat gatcttgttg tcgatgcgct gctcgatgat gtgcgccgcc
    10741 cagcccgagg tccgggcgat cacgaac
//
LOCUS       DAOQVY010000077        10763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_6881_length_10763_cov_5.261300, whole genome shotgun sequence.
ACCESSION   DAOQVY010000077 DAOQVY010000000
VERSION     DAOQVY010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10763
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6881_length_10763_cov_5.261300"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /gene="prs"
                     /locus_tag="PKN91_08200"
     CDS             complement(<1..819)
                     /gene="prs"
                     /locus_tag="PKN91_08200"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNS28092.1"
                     /translation="MLLFAPHHSGALAGRIADALGTPLSASEEREFDGGEHKMCPLQD
                     VRGRDVVVIQSLNGDAQASANDKLCRLLFFIGALKDAGAGRVTACVPYLAYARKDRRT
                     QPRDPVTTRYVAALFEAVAVDRVIVLDVHNEAAFDNAFRCETIRVEAAGVFAAQLAQR
                     DAATRLVVASPDPGGVKRAQRFREALEQACGRSVDFSFMEKRRALGVVSGETFTGDVG
                     DSNVVIYDDLIASGTTLLRAVHAARSAGARRIDVIATHAAFLPAATQLFTDEGPD"
     gene            1106..2875
                     /gene="acsA"
                     /locus_tag="PKN91_08205"
     CDS             1106..2875
                     /gene="acsA"
                     /locus_tag="PKN91_08205"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003313.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNS28093.1"
                     /translation="MPDVPRWPPITGPRKGAGPMANLRDYDSARRAFSWAAVRAELGL
                     APDGPLNMAQIAIDRPAQATPDKLAVRFIDARLNTVDFTYRALQREANRFAQLLASHA
                     VGRGAVVATLLGRSPELFIALLGTLKGGATCTSLFSAFGPEPIRSRLDLAAASVLVTT
                     ARLYERKVAPIRPSLPSLRHVFVVRDDAATPLPAGTIELAAALAQQSDELATASTHQD
                     DAALLHFTSGTTGKPKGVVHAHRAVIAHYATARLALDLRPDDVFWCTADPGWVTGISY
                     GVIAALSCGVSLLVDREEFDVERWYTLLDQQQVSVWYTAPTAIRMLMKAGFAPLRPHR
                     FPALRFIASVGEPLNPEAVMWGVDAFGLPVHDTWWQTETGAIMISNFLSEPVWPGSMG
                     RAMPGADIAIGRRTPDGAIELLGADQVGEIVLRPDWPSMFQTYLNDEARYRGSFCDGW
                     YLTGDEARCDAQGRFWFVGRSDDVIKSAGHLISPFEVECVLNLHPAVVQSAVIGLPDP
                     IAGQIVKGIIELKPDIPPDAGLRREIVAHGRRLLGAAIAPRDIEFTASLPRTRSGKIM
                     RRLLRARALGLPEGDLSMLESGA"
     gene            2872..3876
                     /gene="pdhA"
                     /locus_tag="PKN91_08210"
     CDS             2872..3876
                     /gene="pdhA"
                     /locus_tag="PKN91_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNS28094.1"
                     /translation="MSAQPAGQHAVTREHGLHLLREMLRIRCFEDRCAELYGAGKIRG
                     FLHLYNGQEAVAVGVMQSLRSTDAIAGTYREHGHAIARGIAPRRLMAELFGKETGCCR
                     GRGGSMHIFDATTRFYGGSAIVGGCLPLAVGLALAEKLQGRDGIAVCFFGEGAAAEGE
                     FHESMNLAALWSLPVLFVCENNLYAMGTALARSQAATDLAARAANYRMRAQAVDGMDV
                     LAVEAAARELVAFVRTERAPAFLEARTYRLRAHSMYDPQLYRSKAEVREWEERGPLVT
                     FARRMEAQGLMSEDDLTALREEANAEVDDAVRFAEQSSFEPVADLQRFTYAEVAHGGD
                     "
     gene            3863..4885
                     /locus_tag="PKN91_08215"
     CDS             3863..4885
                     /locus_tag="PKN91_08215"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNS28095.1"
                     /translation="MAAIEANTRRITYRDAVREGLRDALRRDPAVFLMGEDIGKYGGC
                     FAVTKGFLEEFGEARIRDTPLSESAFVGAGIGAALGGMRPIVEIMTVNFSLLALDQIM
                     NTAATLSHMSGGQFNVPLVIRMASGAGRQVAAQHSHSLEGWYSHIPGLRIVTPATLDD
                     AAGMLATALACPDPVIIFEHMALYNMEGELTAAFGPADIDHAVIRRAGRDVTLITYGA
                     SLYKCLTAAERLAAHGIEAEVIDLRSLRPLDERTFIDSVSRTHRAVIVDEGWRSVGLA
                     AEIGMRIMEGAFYELDAPVQRVCGVEVPMPYASHLEQAALPQVEQIVAAVTGMMAGAA
                     GGAGAG"
     gene            5107..5955
                     /locus_tag="PKN91_08220"
     CDS             5107..5955
                     /locus_tag="PKN91_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNS28096.1"
                     /translation="MGPTASPRRRHARKSRPPRARARQLGIDLAGITGTGAGGAITLE
                     DVAGETQPAAVRTPPPRAQPDMRAVIARAMSRSKREIPHYYLSLTCEFQKARTWLERH
                     NESVAIEERLLPSVLLLKAVALGARAMPAFNGYCEAGEFRAAEHVHVGMAIAMRGGRL
                     VAPAILDADAKSVQVVMRELRDLVSRVRSGHMRGSELASPTITVTALAEEGLDLVMPV
                     IYPPQVAIVGFGSILERPWNVDGQVRVAPLVTISLAADHRVTDGHAGARFLAFIRDRL
                     QAPDQL"
     gene            5952..6236
                     /locus_tag="PKN91_08225"
     CDS             5952..6236
                     /locus_tag="PKN91_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNS28097.1"
                     /translation="MNEAELESRVLAILLEVAPDVDPGSLARDVDFRDQFDFDSMDTL
                     NFAIGLHRAFGIEVPETDYRYLAGLGKAVTYVVQALASESPPPTSSRRAV"
     gene            6414..8030
                     /gene="groL"
                     /locus_tag="PKN91_08230"
     CDS             6414..8030
                     /gene="groL"
                     /locus_tag="PKN91_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNS28098.1"
                     /translation="MAHKQVMFASAAREKILRGATQLADAIRITLGPKSKSVLIQKSY
                     GAPIVCNDGVTIAREIDLPDPGENLGAQMLRQAAERTGDLVGDGTSTATILAHAIFAD
                     GVRNVTAGASAIDIKRGLDRGAGAAMAQLKSLSRPVASRKEKAQIATISAHNDESIGE
                     LVADAMERVGGEGVITVEESKTTETALEVVEGMRFDRGYMSPYFVTDSEKMEAVLEDA
                     FLLLTDRKINIMKDLITLLEQVAKSGRPVLVVAEDIEGEALATLIVNQIRGVLKSAGV
                     KAPGFGDRRKDMLQDMAVLTAGQVISEEAGFKLEDTTVAQLGRAKRIVVGKDDTTIIG
                     GAGDRAAIEGRMAQIRREIAKCTSDYDRERLEERLAKLAGGVAVIRVGAPSESEAKAK
                     KDALDDAISSTKAAVEEGIVPGGGLALLKCIRAVEEAELQCEGDERTGVQILRRALEA
                     PARQIALNSAVDGGVVVSKMIEAASNVGFDAARNRYVDLHEAGIIDPTKVVRVAVENA
                     VSVASVLLLTEATMTEIKEDKPAAAGALEP"
     gene            8305..8613
                     /locus_tag="PKN91_08235"
     CDS             8305..8613
                     /locus_tag="PKN91_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNS28099.1"
                     /translation="MSETDKEYLIKAELPAVKKEDIKITLDNGMITLSGERRQEKEHK
                     DENQIRVESFYGSFSRSFSLPENIDPKAIRAESKDGVLRIRIPKTEAPATKAISIDVK
                     "
     gene            8774..8944
                     /locus_tag="PKN91_08240"
     CDS             8774..8944
                     /locus_tag="PKN91_08240"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNS28100.1"
                     /translation="MTSLDREARAGARRAMIAEAAYFLAEKRRFEPGHEIEDWLTAEA
                     EIARITDAGGVS"
     gene            complement(8937..9605)
                     /locus_tag="PKN91_08245"
     CDS             complement(8937..9605)
                     /locus_tag="PKN91_08245"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNS28101.1"
                     /translation="MRLIGGIFLTAVLSLGLQACGSDSGEPTTSNAQNPTPAPSNRAP
                     TISGAPPTEVVVGQAYDFTPQASDPEGATLTFSIQNRPAWATFSSSTGRLSGTPSVAG
                     VTQGVRIAVTDGTNSVSLPAFNLTVSAGTAPPPVVGSATLNWQPPTERTDGSALANLA
                     GYRIYYGNAPGNYSQSVNLTNPGLTSYVIDNLTGGTWYFTMTAVDSAGLESARTNPVS
                     KTIS"
     gene            9848..>10763
                     /locus_tag="PKN91_08250"
     CDS             9848..>10763
                     /locus_tag="PKN91_08250"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNS28102.1"
                     /translation="MTLRFFRHYINLPTALTAVVELLVLLGAPMVVDRLRGYSTHWGT
                     ALLFAGCALLALSAMGLYSARQRARLKGVVLRAGLAMLLALAMVVIITFFVQPLQPEP
                     RQLVEWGAVSLALLVLSRLIGDWLVDENAFKRVVLVYGAGQRAQSLSQLRRRSDQRGF
                     RVAGYVSAPGEPVLVERGVQSIGPDKQLRDLARQLGVDEIVVAMDDRRREFPVRELLD
                     CRLAGIEVVDLVSFLERETGRVRLDVLNPSWIIFGEGFRRDPLRQTSKRVFDVLAALA
                     LLVVTLPFIVLTAIAIMLEDGPRQPILYR"
BASE COUNT         1790 a         3405 c         3816 g         1752 t
ORIGIN      
        1 gtcgggcccc tcgtcggtga acagctgggt cgcggccggc aggaacgcgg catgcgtcgc
       61 gatgacgtcg atgcgccggg ccccggcact gcgcgcggcg tgcacggcgc gcagtagcgt
      121 cgttccggag gcgatgaggt catcgtagat gacgacgttg ctgtcaccga cgtcgccggt
      181 gaaggtttca ccggatacga caccgagggc gcgccgcttc tccatgaacg aaaaatcgac
      241 gctccggccg caagcctgct cgagtgcctc gcgaaaccgc tgtgcccgct tgacgccgcc
      301 cgggtcgggc gatgccacga cgagcctggt tgcggcgtcg cgctgcgcca gctgcgcggc
      361 aaagacgccg gccgcctcga cccggatcgt ctcgcagcga aacgcattgt cgaacgcggc
      421 ctcattgtgg acgtcgagca cgatcacgcg atccacggcc acagcctcga agagagccgc
      481 gacatagcgg gtggtcaccg gatcgcgggg ctgcgtgcgg cgatccttgc gggcgtacgc
      541 gagataaggc acgcaagccg tcacccggcc ggcgcccgca tctttcagtg cgccgatgaa
      601 gaacagcagg cggcagagct tgtcgttggc ggaagcctgc gcatcgccat tcaggctctg
      661 gatcacgacc acgtcgcggc cgcgcacgtc ctgcaggggg cacatcttgt gctcgccacc
      721 gtcgaattcg cgttcctcac tggcggaaag cggcgtgccc agcgcatcgg cgatccggcc
      781 ggcgagcgct ccggagtggt gtggcgcgaa gagcagcatt gtattcatct tattccagat
      841 tcgctccgcc ggccggccgc catagtcatt ctccgggccc ggcttcgggc atttacccaa
      901 ccgctgtggc gatagcggtt atccattgcc gcctcgtgaa caaccctgat tggtgccggg
      961 cgcgatcccg tcgagactcg gcaatgcgtc gggagagatg agaattgatc aatacgtgca
     1021 aagaggggtg gctgccgtgg gcggcgcatg cggtcaacct cgagaagcca catcgggtac
     1081 gggcgaggta tatgtgacgg cggccatgcc cgacgtgccg cgctggccgc cgatcaccgg
     1141 accccgcaag ggcgcgggcc cgatggcgaa cctgcgcgat tatgacagcg cccgtcgcgc
     1201 gttctcgtgg gcagcggtgc gcgccgagct cgggttggcg ccggatggtc cattgaacat
     1261 ggcgcagatc gccatcgacc ggcccgcgca ggccacgccg gacaagctcg cggtccgctt
     1321 catcgacgcg cgtctgaaca ccgtcgactt cacataccgg gcgctgcagc gagaggcgaa
     1381 tcgcttcgca cagctgctgg caagccatgc cgtcggacgc ggcgcggtcg tggcgacgct
     1441 gctcgggcgc agtccggaac tcttcattgc cctgctgggc acgctcaagg gcggtgcgac
     1501 gtgcacctcc ctgttctcgg cattcggtcc cgagccgatc cgctcgcgcc tcgacctggc
     1561 ggccgccagc gtgctcgtga ccacggcgcg cctttacgag cgcaaggtgg cgccgatccg
     1621 gccgtccctg ccgtccctgc ggcacgtttt cgtggtgcgg gacgacgcgg cgaccccgtt
     1681 gcccgctggc accatcgaac tggctgcggc cctcgcacaa caatcggatg agctggccac
     1741 tgcgtcgaca catcaggacg acgcggcgtt gctgcacttc acgagcggga ccaccggcaa
     1801 gcccaagggt gtcgtgcatg cgcatcgggc ggtgatcgcg cactatgcca ccgcgcgcct
     1861 cgcgctggac ctccgccccg acgatgtgtt ctggtgcacg gcggacccgg gctgggtcac
     1921 cggcatctcg tatggcgtga tcgcagcgct cagctgcgga gtgagcctgc tcgtggaccg
     1981 cgaagagttc gatgtcgagc gctggtacac gctgctcgac cagcagcagg tttccgtctg
     2041 gtacacggct ccgaccgcga tcaggatgct gatgaaggcc ggtttcgcgc cgctgcggcc
     2101 gcatcgcttc ccggccctgc ggttcatcgc gagcgtcggc gagccgctca accccgaggc
     2161 ggtcatgtgg ggggtcgatg cctttggcct gccggtgcac gacacgtggt ggcagaccga
     2221 gaccggggcc atcatgatct cgaacttcct gtcggagccg gtctggcccg gctccatggg
     2281 gcgggccatg cccggcgcgg acattgcgat cgggcggcgc accccggacg gcgcgatcga
     2341 gctgctcggc gcggatcagg ttggcgagat cgtgctgcga cccgactggc cgtcgatgtt
     2401 ccagacctat ctgaacgatg aggcgcgcta tcggggcagt ttctgcgacg gttggtatct
     2461 cacgggcgac gaggccaggt gcgacgcgca gggcaggttc tggttcgtcg ggcgcagcga
     2521 cgacgtcatc aagtccgccg ggcatctgat cagtccgttc gaggtggaat gcgtgctgaa
     2581 cctgcatccg gcagtcgtgc agtccgccgt catcggcctg ccagatccga tcgcgggaca
     2641 gatcgtcaag ggcatcatcg agctgaaacc ggacatcccg cccgatgcgg ggttgcgccg
     2701 cgaaatcgtg gcgcatgggc ggcgcctgct cggagcggcc atcgcgccgc gcgacatcga
     2761 gttcacggcc tcgctgccac gcacgcgcag cggcaagatc atgcgccgcc tgttgcgggc
     2821 acgcgcgctc ggtctgcccg aaggggacct gtccatgctg gaatcgggcg catgagcgcg
     2881 cagccggccg ggcagcatgc agtgacgcgc gagcacggct tgcacctgtt gcgcgaaatg
     2941 ttgcgcatcc gctgcttcga ggatcgctgc gccgagctgt acggcgccgg caagatccgc
     3001 ggcttcctgc acctgtacaa cggtcaggag gcggtcgccg tcggcgtgat gcagtcgctg
     3061 cggtccaccg acgcgatcgc ggggacctat cgcgagcacg gtcacgccat cgcacgtggt
     3121 atcgccccgc gcaggctcat ggcggagctg ttcggcaagg agacggggtg ttgtcgggga
     3181 cggggcggat cgatgcatat cttcgatgcg acgacgcggt tctacggggg cagtgccatc
     3241 gtcggcggat gcctgccgct ggccgtgggc ctcgcgctgg cggaaaagct tcagggccgc
     3301 gacggcatcg cggtctgctt cttcggcgag ggcgcggccg ccgaggggga gttccacgag
     3361 tcgatgaacc tcgctgcgct gtggagcctg ccggtcctgt tcgtctgcga gaacaaccta
     3421 tacgcgatgg ggaccgcgct cgcgcgttcc caggccgcaa ccgacctggc ggccagggcc
     3481 gcgaactacc gcatgcgcgc gcaggcggtg gacggcatgg acgtcctggc cgtggaggcg
     3541 gcggcgcggg aactggtggc attcgtgcgg acggagcgcg cgcccgcgtt cctcgaggcg
     3601 cgcacgtatc gattgcgcgc gcactcgatg tacgacccgc agctctatcg cagcaaggcc
     3661 gaggtcaggg aatgggagga gcgcgggccg ctggtcacgt tcgcgcgccg tatggaggcg
     3721 cagggactga tgtccgagga tgacctgacg gccttgcgag aggaagccaa cgcggaggtg
     3781 gacgatgcgg tccggtttgc ggagcagtca tcgttcgagc cggtggccga cctgcagcgc
     3841 ttcacctacg cggaggtcgc ccatggcggc gattgaggcg aacacgcgtc gcatcaccta
     3901 ccgcgacgcg gtcagggagg ggctccgcga cgcattgcgg cgcgatccgg cggtcttcct
     3961 gatgggagaa gacatcggca agtatggcgg ctgctttgcc gtcacgaagg gattcctcga
     4021 ggagttcggc gaggcccgca tccgcgacac gccactgtcc gaatccgcgt tcgtcggcgc
     4081 gggtatcggc gctgcgctcg gcggcatgcg gcccatcgtc gagatcatga ccgtcaactt
     4141 cagcctgctc gcgctcgacc agatcatgaa tacggcggcc acgctgagcc acatgtcggg
     4201 gggacagttc aacgtgccgc tggtcatccg gatggcgagc ggagcgggtc ggcaggtggc
     4261 cgcgcagcac tcgcacagcc tggaagggtg gtacagccac atccccggcc tgcgcatcgt
     4321 cacgccggcc acgctcgacg acgcggccgg catgctggcc actgcgctcg catgccccga
     4381 tccggtgatc atcttcgagc acatggcgct atacaacatg gaaggcgaac tgactgcggc
     4441 gttcgggccc gccgacatcg accacgccgt gatccggcgg gcgggcaggg acgtgacgct
     4501 catcacctac ggcgcgtcgc tctacaagtg cctgacggcg gcggaacgac tggcggcgca
     4561 cgggattgaa gccgaggtga tcgacctgcg ttcgctgcga ccgctcgatg agcggacctt
     4621 catcgattcc gtgagccgca cgcatcgcgc ggtgatcgtc gacgaggggt ggcgcagtgt
     4681 cgggctcgcc gcggagatcg gcatgcggat catggagggc gccttctacg agctcgacgc
     4741 gccggtgcag cgcgtgtgcg gcgtggaggt gccgatgccg tacgcgagcc atctcgaaca
     4801 ggcggccctg ccgcaggtcg aacagatcgt cgcggcggtc acggggatga tggccggtgc
     4861 ggcgggtggt gcgggtgccg gctgagtttc gcatgccctc gctggggccg gacatggagt
     4921 ccggcacgct ggtcgagtgg cgggtcaagc cgggtgacgt cgtgaagcgc ggcgacatcg
     4981 tcgcgctcat cgaaacggac aaaggcatca tcgacgtcga gatcttctcg gagggcagga
     5041 tagcgaaact gctggtggag gccggtaccc gcgtcccggt cggtaccgtg ctcgcgcagt
     5101 tggacggtgg gaccgacggc gagtccgcgc cgccggcacg ccagaaagtc tcgcccgccg
     5161 cgcgcgcgcg cgcggcagct cggcatcgac ctcgccggga tcacgggcac cggcgcgggc
     5221 ggcgcaatca ctctcgagga tgtcgcgggc gagacgcagc cggccgcggt ccgcaccccg
     5281 ccgccccgcg ctcaacccga catgcgggcg gtcatcgcca gggccatgtc gcgctccaag
     5341 cgcgagattc cccactacta cctgtcgctc acctgcgagt tccagaaggc gaggacgtgg
     5401 ctcgagcgcc ataacgagag cgtggccatc gaagaaaggc tgctgccgtc ggtgctgctg
     5461 ctcaaggcgg tcgcgctcgg cgcgcgggcg atgcccgcgt tcaatggcta ctgcgaggcg
     5521 ggcgaattcc gggctgcgga gcatgtccat gtcggtatgg ccattgcgat gcggggcggg
     5581 cggctcgtcg cgccggccat cctcgacgcg gacgcaaaga gcgtgcaggt cgtcatgcgt
     5641 gagctccgtg acctggtgag ccgggtgcga tcgggccata tgcgcggcag cgaactcgcg
     5701 tcgccgacga tcacggtgac cgcccttgcc gaggagggcc tggacctggt catgccggtc
     5761 atctatccgc cacaggtggc gatcgtggga ttcggctcca ttctcgagcg accctggaac
     5821 gtggacggcc aggttcgggt ggcaccgctc gtgacgatca gcctggccgc ggatcatcgc
     5881 gtcaccgacg gccacgccgg tgcgcgcttc cttgcattca tccgtgaccg attgcaggca
     5941 ccggatcagc tatgaacgaa gccgagctcg aaagtcgggt actcgcgatc ctgctcgagg
     6001 tggcccccga cgtcgatccc ggcagcctcg cgcgtgatgt cgactttcgt gaccagttcg
     6061 acttcgactc catggatacg ctcaacttcg cgatcggctt gcacagggcg ttcggcatcg
     6121 aagtgccgga aaccgactac cgctatctcg cgggcctcgg caaggcggtc acctacgtcg
     6181 tacaggcgct cgcgtcggag tcgccgccgc ctacctcgtc gcgacgcgca gtctgaccgc
     6241 gacgaggctc ccctgactcg ccgacagcga gtcgatgccg gctcgcctga cgacttcgcg
     6301 ggttcttccg ggccgcgatc acgggaaacc gcaagcggca tgcagggttc ccggagcccc
     6361 attctcccgg cgggcatgca gactccgtgt cgttgccggg caggagcagg tgcatggcgc
     6421 acaaacaggt gatgttcgcg tcggccgccc gcgagaagat tctgcgcggc gcgacacaac
     6481 tcgcggatgc catacgcatc acgctcggcc cgaaatccaa gtcggtcctg atccagaaat
     6541 cgtacggggc accgatcgtc tgcaacgacg gcgtgacgat cgcgagggag atcgacctgc
     6601 cggacccggg ggagaatctc ggcgcgcaga tgctgcgaca ggccgccgag cgaaccggcg
     6661 acctcgtcgg cgacggcacg agcacggcca cgatcctcgc gcacgcgatc ttcgccgacg
     6721 gtgttcgcaa tgtgacggcc ggcgccagcg cgatcgacat caagcgcggg ctcgaccggg
     6781 gcgccggggc agcgatggcg caactcaaga gcctgtcgag gcccgtcgcg agccgcaagg
     6841 agaaggccca gatcgcgacg atctcggctc acaacgacga gagcatcggt gaactcgtgg
     6901 cggacgccat ggagcgggtg ggcggggagg gcgtgattac ggtcgaggaa tcgaagacga
     6961 ccgagaccgc actcgaagtc gtcgagggca tgcgcttcga ccggggctac atgtcgccgt
     7021 acttcgtgac ggattcagag aagatggaag cggtgctgga ggatgctttc ctgctgctga
     7081 ccgatcgcaa gatcaacatc atgaaggatc tgatcacgct gctcgagcag gtcgccaaga
     7141 gtgggcgacc tgtgctggtc gtcgcggagg acatcgaggg cgaggcgctc gcgaccctga
     7201 tcgtcaacca gatccgtggc gtcctgaaga gcgcaggcgt caaggcgccc ggcttcggcg
     7261 accggcgcaa ggacatgctg caggacatgg ccgtgctgac cgcgggccag gtcatctccg
     7321 aagaggccgg gttcaagctg gaggacacga ccgtcgcgca actgggccgc gccaagcgga
     7381 tcgtcgtcgg caaggacgat acgacgatca tcggcggcgc gggggaccgt gccgcgatcg
     7441 aggggcgcat ggcgcagatt cgccgtgaaa ttgcgaagtg cacgagcgat tacgaccggg
     7501 agcggctgga ggagcggctg gccaagctcg ccggcggggt cgcggtcatt cgcgtgggag
     7561 cgccctccga gtcggaggca aaggcgaaga aggacgcgct cgacgacgcg atcagttcca
     7621 cgaaggccgc cgtcgaggag gggatcgtcc cgggcggggg actcgcgctc ctgaaatgca
     7681 tccgtgcggt cgaagaggcc gagctgcaat gtgaaggaga cgaacgcacg ggggtgcaga
     7741 tcctgaggcg ggcgctggag gcgccagcgc ggcagatcgc gttgaactcg gcagtggacg
     7801 gcggggtcgt ggtgagcaag atgatcgagg cggcctcgaa tgtcggtttc gacgcggcgc
     7861 gcaaccggta cgtggatctc catgaggcgg gcatcatcga tccgacgaag gtcgttcgcg
     7921 tcgcggtgga gaacgccgta tcggtagcga gcgtgctgct cctgaccgag gcgaccatga
     7981 ccgagatcaa ggaggacaag cctgctgcag ccggagcgct cgagccctga gccctgcctg
     8041 ggaagtcgcg gtcgcccacg agtcccgccc gaacttcatg aatccccgta acgactacga
     8101 cctttcccga atgggcggcg cctgcgcgac ctgtgagcat ttgcagtacg aggggagata
     8161 gaccgcagcc aggagggtat cgtgaacatc acgcgttggg agcctttccg tgaaatggag
     8221 gatttcttcc gccagtactc gccgttcttc gctcgcgtgc atcgcaacag cgacgagccg
     8281 gtggagtaga cgccggcagc tgacatcagc gaaaccgaca aggaatatct gatcaaggcc
     8341 gagctgccgg cggtgaagaa ggaggacatc aagatcacgc tcgacaacgg catgatcacg
     8401 ctcagcgggg aacgccggca ggagaaggag cacaaggacg agaaccagat ccgcgtggag
     8461 agcttctacg ggtccttctc gcgcagcttc tcgctccccg agaacatcga tccgaaggcg
     8521 atccgcgcgg agagcaagga tggcgtactg cgcattcgga tccccaagac ggaggcgccg
     8581 gcgaccaagg cgatctcgat cgatgtcaag tgagcgcgac gagggtagtc gaggcgggac
     8641 ggcgaacgtg aatgcacggg tgcagcaccg gcgaaggagc gggaactccg gtgcaggcaa
     8701 gcctgtcgtc gcggtcgccc ggccccacca cgaggtgaca gaggatcacc ggcgggaggc
     8761 gggcgccttt cacatgacgt cgctcgatcg cgaggcacga gccggggcgc gtcgcgcgat
     8821 gattgccgag gcggcgtact ttctcgcgga gaagcgccga ttcgagccgg ggcacgagat
     8881 cgaggactgg ctcaccgccg aagcggagat cgcccgcatt acggatgctg gcggcgtcag
     8941 ctgatcgtct tcgataccgg gttggtgcgc gcgctttcca ggcccgcgga gtcgaccgcg
     9001 gtcatcgtga agtaccacgt gccgcccgtg aggttatcga tcacgtagct ggtgaggccc
     9061 gggttcgtca ggttgacgct ctgggaatag ttgcccggcg cattgccgta gtaaatgcgg
     9121 tagccggcga ggttggcgag cgccgagcca tcggtgcgtt cggtgggcgg ctgccagttc
     9181 aaggtggcgc tgccgaccac cggcgggggt gccgtgcccg cgctcaccgt gagattgaag
     9241 gccgggaggc tgacggagtt cgtgccatcg gtcacggcga tgcgcacgcc ctgggtgacg
     9301 ccggccacgc tgggcgtgcc gctgaggcga ccggtggacg agctgaacgt ggcccacgca
     9361 ggcctgttct ggatcgagaa ggtgagggtc gcgccttccg ggtcggaggc ttgcggcgtg
     9421 aaatcgtagg cctgcccgac gaccacctcg gtcggcggcg cacccgaaat cgtcggcgcg
     9481 cgattgctcg gtgcgggagt cgggttctgg gcattcgacg tcgtaggttc accggagtcg
     9541 ctgccgcaag cctgcaggcc gaggctgagt actgccgtca agaagatgcc gccgatcaat
     9601 ctcatcagat ttcccccact ccacgcgttg gtcaggaagt gctttgccgt cgtggtgcaa
     9661 caagatatgt ggcggacgga gggcctacaa tatggctaag cgtctgtatt cggagacatt
     9721 ttcggtacag tgaaacgcgt ctcatggcag tattcgctcg ccgcgacagg cagttacgcg
     9781 aacttgctca cgtcgggtgt tttggaacgt caatagactg cgctgcgccg taagctggca
     9841 ggtgtcgatg accctacgtt tcttccgcca ttacataaac ctgcccactg cgctgacagc
     9901 cgtggtcgaa ttgctggtac tgctgggcgc accgatggtg gtggaccgcc tgcgcggcta
     9961 ttcgactcac tggggcacgg cgctcctgtt cgccgggtgc gcactgcttg cgctgtcggc
    10021 catgggactc tacagcgccc gccagcgcgc gcggctcaag ggtgtggtgc tgcgcgccgg
    10081 actcgcgatg ttgctcgcgc tggcgatggt cgtgatcatc acgttcttcg tccagcccct
    10141 gcagcccgag ccgaggcaac tcgtcgaatg gggagccgta tcgcttgcgc tgctcgtcct
    10201 ttcacggctg atcggcgact ggctggtcga cgagaatgcc ttcaagcgcg tcgtgctggt
    10261 ctatggcgcc gggcaacggg cgcagagcct ctcgcagctg cggcgccgtt ccgaccagcg
    10321 cggctttcgg gtcgccggct acgtgtcggc gcccggcgaa cccgtgctcg tcgagcgcgg
    10381 cgtgcaatcg atcggcccgg acaaacagct gcgcgacctg gcccggcaat tgggcgtcga
    10441 cgagatcgtg gttgcgatgg atgatcggcg gcgtgagttc ccggtgcgcg agttgctgga
    10501 ttgccggctc gcaggcatcg aagtggtcga tctggtcagc ttcctcgagc gggaaaccgg
    10561 tcgtgtgcgg ctcgacgtgc tgaacccgag ctggatcatc ttcggcgaag gcttcaggcg
    10621 cgacccgctc cgccagacct cgaagcgcgt gttcgacgtg ctcgctgcac tcgcgctgct
    10681 cgtcgtgacc ctgccgttca tcgtgcttac cgcgatcgcg atcatgctcg aggatggtcc
    10741 gcgccagccg atcctgtatc gac
//
LOCUS       DAOQVY010000078        10549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_7056_length_10549_cov_5.426434, whole genome shotgun sequence.
ACCESSION   DAOQVY010000078 DAOQVY010000000
VERSION     DAOQVY010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10549
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7056_length_10549_cov_5.426434"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..397
                     /locus_tag="PKN91_08255"
     CDS             17..397
                     /locus_tag="PKN91_08255"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HNS28103.1"
                     /translation="MIMHFRRGIVALTLLAFVASVAPTPASAAMIGTQAAAAAGARAA
                     NLARVDGVLARADVQQRLTDLGVDPDAARVRAAALSDAELASFAEQLEQAPAGGDILA
                     VIGIVFVVLLILEFTGVIDIFKKA"
     gene            414..908
                     /locus_tag="PKN91_08260"
     CDS             414..908
                     /locus_tag="PKN91_08260"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HNS28104.1"
                     /translation="MQRVRFGAVAVALLASGCSLLASHGSGPGADAPWPADAPPAVEL
                     AAVPFHPQDEYQCGPAALATLLGASGIGVAPDDLVAEVYVPARKGSLQTELLAATRQR
                     GRIALVLEPSMAALVHELAAQRPVLVLLNLGVRSWPIWHYAVVIGYERDTHALLLRSG
                     TTEC"
     gene            912..1385
                     /locus_tag="PKN91_08265"
     CDS             912..1385
                     /locus_tag="PKN91_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28105.1"
                     /translation="MSLRRFSGAWSRADRWAYVALRAGELPASGGGERYAAAVADFEA
                     VDAGAALRAYEAGIARWPDTPLLRLGAANVLLMKGDPAAAEAMLTDLLRIAPADVAAR
                     NNLAELLSRRGCRDAARAQIALARAAAKGGPLAPIIEQTAAEIESRDADPAAQCP"
     gene            complement(1407..2291)
                     /gene="galU"
                     /locus_tag="PKN91_08270"
     CDS             complement(1407..2291)
                     /gene="galU"
                     /locus_tag="PKN91_08270"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HNS28106.1"
                     /translation="MPQNALIKTAVFPVAGRGTRFLPATKASPKEMLPVVDKPLIQYA
                     VEEALQAGARRLVFITGASKRAIEDHFDSDQELEKLLEAQGKSELVRQLRSVLPSYAS
                     CIYIRQPAPLGLGHAVLCAAPAVGFEPFFVHLADDLIMGEPGCLSQMAEVYSGVRASV
                     LGVEVVPKRETDKYGIVAVERGSGPVRRIRRIIEKPKPTAAPSNLAVVGRYVLSASIF
                     AHLERIGRGAGGEIQLTDGIARLMQTEPVYAYRFTGKRYDCGSKLGYLQATVEHALAH
                     EELGDPFAAYLRRLAKRL"
     gene            complement(2333..2692)
                     /locus_tag="PKN91_08275"
     CDS             complement(2333..2692)
                     /locus_tag="PKN91_08275"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HNS28107.1"
                     /translation="MRSIIACLLLCLALPISAYADPVNINTADAATLDRELKGIGPSR
                     AAAIVAYRMQHGPFKSVDELALVAGIGQKVIDDNREVLRVDRASGKPAPAVKAAPAAG
                     KPAAKSTVKPMRSKPAQ"
     gene            complement(2823..3125)
                     /gene="ihfB"
                     /locus_tag="PKN91_08280"
     CDS             complement(2823..3125)
                     /gene="ihfB"
                     /locus_tag="PKN91_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HNS28108.1"
                     /translation="MTKSELIEIITAKQKHLPAKDVELALKQILEIMSDALATGDRIE
                     IRGFGSFSLHFRPPRQGRNPKTGEAVALAGKYVPHFKPGKDLRERVNDGADKPIRD"
     gene            complement(3205..4890)
                     /gene="rpsA"
                     /locus_tag="PKN91_08285"
     CDS             complement(3205..4890)
                     /gene="rpsA"
                     /locus_tag="PKN91_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNS28109.1"
                     /translation="MTESFAQLFEQSLANQRIRPGMILTGLVVGLTDDIVIVNVGLKS
                     EAVIGIDQFKNERGEIEVAVGDEVEVALDAVEDGNGETRLSREKAKRARTWTRLEKAF
                     EASEIVTGVITGRVKGGFTVEIETVRAFLPGSLVDVRPVRDTSYLEGKPLEFKVIKLD
                     QKRNNVVVSRRAVVEQEFSAERSALMENLQEGSVVRGTVKNLTDYGAFVDLGGIDGLL
                     HITDMAWKRVKHPSEVVKVGDEIDVRILKFDRERSRVSLGLKQLGADPWENIARRYPP
                     NTRVFGKVTNIADYGAFVEIEDGVEGLVHVSEMDWTNKNVNPAKVVQTGQEVEVMVLD
                     VDEERRRISLGLKQCQANPWKEFAENSNRGEKVSGQIKSITDFGIFIGLPGGIDGLVH
                     LSDISWDVPGEEAVRSYQKGQLIEAMVLSIDPERERISLGIKQLAKDPFSAYIAENPR
                     GTIVRGVVREVDARGAVIDLGNGIEGQLRASELGRDRVEDARTVLKEGEEIEAKFTGV
                     DRKTRTISLSIKAKEAHEEAEAVSSYRSDQGAASSGTSLGDLLKEQIGSSDRS"
     gene            complement(5014..5697)
                     /gene="cmk"
                     /locus_tag="PKN91_08290"
     CDS             complement(5014..5697)
                     /gene="cmk"
                     /locus_tag="PKN91_08290"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNS28110.1"
                     /translation="MDAVPVVAIDGPSGSGKGTVSREVARVVGWHLLDSGALYRIVGL
                     LGARRGLSAEDVEGHAALARSMDVVFGSDGQGGERVRVDGVDVTREIRSEEAGQGASR
                     VAAWPAVRDALLDRQRAFARPPGLVADGRDMGTVVFPAARLKIYLVATAEERALRRYK
                     QLKEKGSGANLAALSREIAERDQRDSTRAVAPLVPAPDARIIDSTGMSVAQVVAAVLS
                     QGRSLGLWS"
     gene            complement(5707..7008)
                     /gene="aroA"
                     /locus_tag="PKN91_08295"
     CDS             complement(5707..7008)
                     /gene="aroA"
                     /locus_tag="PKN91_08295"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNS28111.1"
                     /translation="MNRQVRYLVQPGERVGGELTVPGDKSISHRALMLGGIADGRTEI
                     HGFLPSEDCLATLAALQAMGVTVDRTEPTRVCVHGAGVRGLSAPGQVLDVGNSGTAMR
                     LFMGLLAGQAFDTTLTGDASLMTRPMERVARPLREMGAAIETHDGRPPVRITGGRRLH
                     AIDYDLPVPSAQVKSAILLAGLQAAGTTRVTEPAPTRDHTERMLQGFGVRVQRDGATV
                     AIEGGQRLTATRVDVPADISSAAFFIVAGCLAAHEGLLLRSVGMNPTRTGVIDILRLM
                     GADISVEGARVSGGEPSADLRIKAAPLRGVAIPEALVPLAIDEFPVLFIAAAAAEGVT
                     ELRGAGELRVKETDRLAVMAEGLGRLGVVHELLPDGIRIEGGPMSGGTVDSHGDHRIA
                     MAFAVASLRASGPLEILDVANVATSFPGFVPLARAAGLLIT"
     gene            complement(7005..8147)
                     /gene="hisC"
                     /locus_tag="PKN91_08300"
     CDS             complement(7005..8147)
                     /gene="hisC"
                     /locus_tag="PKN91_08300"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNS28112.1"
                     /translation="MSTEHPARLASECVRQIAPYTPGKPVSEVARELGLTDIIKLASN
                     ENPLGAGPAAMAAMQAALAESAAYPDGSGHALKQALAARLGVAPAQLTLGNGSNDVLV
                     LLAEAFLTPGTDAVFSQYCFAVYPLAVQATGARAIVVPALPADHPRMPLGHDLDAMRA
                     AVTAQTRLVFVANPNNPTGTWVGAAALEAFVAGLPSSTLVVLDEAYFEYARAEDCGDG
                     IAWLARHPRLVVVRTFSKAYGLAGLRVGYAASHPEVADVLNRIRQPFNVNSIALEGAR
                     AALADDEHLVRSVALVQAERPRLARELASRGFTVLPSAGNFLLAHIGSAPRAAALNDH
                     LLRGGVIVRPVGTYGLAEYLRITIGRPQENARLLELIDAWPGDGAR"
     gene            complement(8144..9316)
                     /gene="pheA"
                     /locus_tag="PKN91_08305"
     CDS             complement(8144..9316)
                     /gene="pheA"
                     /locus_tag="PKN91_08305"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNS28113.1"
                     /translation="MAGRKATAKPKPKPKPMARAKGKAKRSAPGRVALDDVRRRIDDV
                     DARIHDLLNERAALAQQVGISKHKDGHTVDFYRPEREAQVLRLALERNKGPLRDEEVL
                     RLFREIMSACLAQQEPLKVAFLGPEGTFTQAAVLKHFGHSVRALALASIEEVFHEVEA
                     ASADFGVVPIENSSEGTVNYTLDRFLSSPLHICGEVELRIHHFLMGRMDSLRKVKRIC
                     SHPQSLAQCRDWLRENLPDIELVPVASNAEGARRARDEQGTAAIAGDTAAEVYGLNIL
                     AAQIEDRPDNTTRFLVIGRKLFAPSGVDRTTFIVSGGHTDAPGALYRLLEPLARHRIN
                     VTRIESRPSGRRKWDYVFFLDIEGHADDPAIAKALASLKKRASLFRVLGAYPRAIL"
     gene            complement(9333..10502)
                     /locus_tag="PKN91_08310"
     CDS             complement(9333..10502)
                     /locus_tag="PKN91_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNS28114.1"
                     /translation="MTFRIQTLNNISVKGLERLPRERYEIASDIARPDAILVRSADMH
                     ALQLPDSLLAVGRAGAGVNNIPVAALSQRGVPVFNAPGANANAVKELVIAGMLIAARN
                     VCAAWDFARSVTGDDKEIDVAVEKGKKNFVGFELPGRTLGVVGLGAIGVEVANSSLAL
                     GMKVLGFDPQITVQRAWQLSSNVEQALSLDDLFSRADIVTVHVPLNDQTRGLVNEARL
                     ALMRKGGVVLNFARAPIVDDKAIIASLDAGHLRAYVCDFPKNALKDHPRIVTLPHLGA
                     STGEAEENCAVMVAETVRDFLENGNLRNCVNFPEAVLSRVPGTSRIAIANSNVPTMIG
                     QISNCLAEAKLNIADLLNKSRGEYAYTLIDADGEVPAGVVERLRAIHGVLSARVV"
BASE COUNT         1599 a         3648 c         3576 g         1726 t
ORIGIN      
        1 cacttcagga gaatccatga tcatgcactt tcgtcgcgga atcgtcgcac tgaccctcct
       61 ggctttcgtt gcctcggtgg cgccgacccc ggccagtgcc gcgatgatcg ggacccaggc
      121 tgcggctgcg gccggtgctc gcgcggccaa cctcgcgcgc gtggacggcg tgctggcgcg
      181 cgccgatgtg cagcagcggc tcaccgacct cggcgttgat ccggatgctg cgcgtgtccg
      241 cgcggcggcg ctgagcgacg cggaactcgc cagcttcgcc gagcaactcg agcaggcgcc
      301 ggcaggtggc gacatcctcg ccgtgatcgg catcgtgttc gtcgtgctgc tgatcctgga
      361 gttcaccggc gtcatcgaca tcttcaagaa agcctgacgc cggcaggcgc gccttgcagc
      421 gcgtccgctt tggggctgtc gccgtcgcgc tgctggcgag cggatgcagc ctgctggcca
      481 gccacggcag cggacccggc gcggacgcgc cgtggccggc cgacgcgccg cccgcagtcg
      541 aacttgcggc ggtgccgttc catccgcagg acgaatacca gtgcggcccg gccgcgctcg
      601 cgaccctgct cggcgcaagc ggcatcgggg tggcgcccga cgacctggtc gccgaggtct
      661 atgttcccgc ccgcaagggc agcctgcaga ccgagctgct cgccgcgacg cgccagcgcg
      721 ggcgcatagc cctggtgctc gagccgtcga tggcggcgct ggtccacgaa ctcgccgcgc
      781 agcgccccgt gctcgtgctc ctcaacctcg gcgtgcggtc gtggccgatc tggcactacg
      841 cggtcgtcat cggctacgaa cgggacacac acgcgctgtt gctgcgctcc ggcacgaccg
      901 agtgttagcg catgtcgctg cggcgctttt ccggcgcctg gtcacgggcg gatcgctggg
      961 cctacgtggc gctgcgcgcc ggggaattgc cggcgagcgg tggtggcgag cgctatgccg
     1021 ccgcggtcgc cgatttcgag gcagtcgacg ccggcgcggc gctgcgggcg tatgaggccg
     1081 gcattgcgcg ctggcccgac acacccctgc tgcgtctcgg cgccgccaat gtgctgctca
     1141 tgaagggtga cccggccgcg gccgaagcca tgctcacgga cctcttgcga atcgcgcccg
     1201 ccgatgtggc cgcgcgcaac aatctcgccg agttgctgtc gcggcgcggc tgccgggacg
     1261 cggcgcgcgc gcagatcgcg ttggcgcgcg ccgctgcgaa aggcggcccg ctcgcaccga
     1321 tcatcgaaca gaccgccgcg gagatcgaat cacgcgacgc ggaccctgca gcgcagtgcc
     1381 cgtgagtccc gcggcaccgc gctgcgctag aggcgcttcg cgagcctgcg cagataggcc
     1441 gcaaaggggt cgccgagttc ctcgtgggcg agtgcgtgct cgacggtcgc ctgcaggtaa
     1501 ccgagcttgc tgccgcagtc gtagcgcttg cccgtgaagc gataggcata gaccggctcg
     1561 gtctgcatca gccgcgcaat gccgtcggtc agctggatct cgccgcccgc gccgcgtccg
     1621 atgcgctcga ggtgcgcgaa gatgctcgcc gagagcacat agcggccgac gaccgcgagg
     1681 ttcgagggtg ccgcggtcgg cttgggcttc tcgatgatcc tgcgtatgcg gcgcaccggc
     1741 cctgatccac gctcgacggc gacgatgccg tacttgtcgg tttccctctt cggcaccact
     1801 tcgacgccca gcacgctcgc acgcactccg gaatacacct ccgccatctg cgacaggcag
     1861 cccggctcgc ccatgatcag gtcgtcggcc aggtgcacga agaacggctc gaagcccacg
     1921 gccggtgcgg cgcacagcac ggcatggccg aggccgagcg gcgcaggctg gcggatgtag
     1981 atgcacgacg catacgaggg cagcacgctg cgcagctggc gcacgagctc gctcttgccc
     2041 tgcgcctcga gcagcttctc gagttcctgg tcggaatcga agtgatcctc gatcgcccgc
     2101 ttcgacgcgc cggtgatgaa cacgaggcgc cgcgcacccg cctgcagcgc ttcctcgacc
     2161 gcatactgga tgagcggctt gtcgaccacc ggcagcattt ccttgggact ggccttggtc
     2221 gcaggcagga agcgggtacc gcggcccgcc acggggaaga ccgcggtctt gatcaacgcg
     2281 ttctggggca tgcgccgatt ctaacgcagc gtgcgcatgc gccacggcgt gctcactgcg
     2341 ccggcttgct gcgcatcggc ttgactgtcg acttggcggc cggcttgccc gcggccggcg
     2401 cggccttgac cgccggcgcc ggcttgccgc tggcccgatc gacccgcagc acttcccgat
     2461 tgtcgtcgat gaccttctgg ccgatgccag cgaccagcgc cagttcatcg acgctcttga
     2521 acgggccgtg ctgcatgcga tacgcgacga tcgcggcggc gcgcgagggc ccgatgccct
     2581 tcagctcgcg atcgagcgtg gccgcatcgg cggtgttgat gttgaccgga tcggcgtacg
     2641 cgctgatcgg cagcgcaaga cacagcagca gacaggcaat gatggaacgc atggctctcc
     2701 cctccggatg tgtggcggcc gacggccgca tggggagagg ctagtgcggt ggacacgcgc
     2761 agccgagcgt cgaaagtgcg gcaaacgcgc cgcttcaggg cttgaccgca gcgggcggga
     2821 gctcagtccc ggatcggctt gtccgcgccg tcattgacgc gctcgcgcag gtccttgccc
     2881 ggcttgaaat gcggcacgta cttgccggcg agcgccactg cctcgccggt cttcggattg
     2941 cgcccctggc ggggcggacg gaaatgcagc gagaagctgc cgaaaccgcg gatctcgatg
     3001 cgatcgccgg tcgcaagcgc atcgctcatg atctcgagga tctgcttcag cgccagctcg
     3061 acatccttcg ccggaagatg cttctgcttc gcagtgatga tttcgatgag ttccgatttc
     3121 gtcatgcgct gctgttaccc ccggcgcgtg ggcgcccgga cttgcggtcc gggcgcctcg
     3181 cccttgtcgt ttcttgtcag cgtatcagga tcggtccgac gaaccgatct gctccttcag
     3241 caggtcgccg agcgacgttc ccgacgaagc agcgccctgg tcggaccggt agctcgacac
     3301 ggcttcggcc tcttcatggg cctccttggc cttgatcgac agcgagatcg tgcgcgtctt
     3361 gcgatcgacg cccgtgaact tcgcctcgat ctcctcgcct tccttcagca cggtgcgcgc
     3421 gtcctcgacg cgatcgcggc cgagttccga ggcgcgcagc tggccctcga tgccgttgcc
     3481 gaggtcgatc accgcgccgc gcgcatcgac ttcacggacc acgccccgca cgatcgtgcc
     3541 gcgcggattc tcggcgatgt aggccgagaa cggatccttg gcgagttgct tgataccgag
     3601 cgaaatgcgt tcgcgctccg ggtcgatcga cagcaccatc gcctcgatca gctggccctt
     3661 ctggtagctg cgcacggctt cctcgccggg cacatcccag gagatgtccg agaggtgcac
     3721 gaggccgtcg atcccgccgg gcaggccgat gaagatgccg aagtcggtga tcgacttgat
     3781 ctgccccgag accttttcgc cgcggttgct gttctcggcg aattccttcc acgggttcgc
     3841 ctggcattgc ttgaggccga gcgagatgcg gcgacgctcc tcgtcgacgt cgagcaccat
     3901 gacctcgact tcctggccgg tctgcacgac ctttgccgga ttgacgttct tgttggtcca
     3961 gtccatttcg gacacgtgca cgaggccctc gacgccatcc tcgatctcga cgaacgcgcc
     4021 gtagtcggcg atgttcgtga ccttgccgaa cacgcgcgtg ttgggcggat agcggcgcgc
     4081 gatgttctcc caaggatctg cgcccagctg cttgaggccg agcgagacac gcgagcgctc
     4141 gcggtcgaac ttcaggatgc gcacgtcgat ctcgtcgccc accttgacga cttccgacgg
     4201 atgcttgacg cgcttccacg ccatgtcggt gatgtgcagc aggccgtcga tgccgccgag
     4261 gtcgacgaac gcgccgtagt cggtgaggtt cttgaccgtg ccgcgcacga ccgagccttc
     4321 ctgcaggttc tccatgagcg cgctgcgctc tgcggagaac tcctgctcca cgaccgcgcg
     4381 gcgcgacacg accacgttgt tgcgcttctg gtcgagcttg atgaccttga actcgagcgg
     4441 cttgccctcg aggtaggaag tgtcacgcac cgggcgcacg tcgaccagcg aacccggcag
     4501 gaacgcccgc acggtctcga tctcgacggt gaagccaccc ttgacgcgac cggtgatcac
     4561 gccggtgacg atttcgctcg cctcgaacgc cttctcgagg cgggtccagg tgcgggcgcg
     4621 cttggccttc tcgcgcgaca ggcgggtctc gccgttgccg tcctcgacgg catcgagcgc
     4681 gacttccact tcgtcgccga ctgcgacctc gatctcgccg cgctcgttct tgaactgatc
     4741 gatgccgatc acggcctcgg acttgaggcc cacgttcacg atgacgatgt cgtcggtgag
     4801 cccgacgacg agtccggtga ggatcattcc cggacggatg cgctggttgg cgagactctg
     4861 ctcgaatagc tgagcgaaac tttcagtcat gttggttgat gtcctgcatg cgcggccttg
     4921 ccgcgctgta acaccccggc ccgttccttg agccctgggg aaacccgaaa aaagcgctgc
     4981 atcccgcagc gccacgtcca attcctcgcg cgttcagctc cacaggccga gactgcgtcc
     5041 ctgcgacagc accgccgcga cgacctgagc cacgctcatg ccggtcgaat cgatgatgcg
     5101 ggcgtcgggc gcaggcacca gcggtgcgac cgcgcgggtc gaatctcgct ggtcccgttc
     5161 ggctatctca cgcgaaaggg cggcgaggtt agcaccagac cccttttctt tcaactgctt
     5221 atagcgcctc agggcgcgct cttccgccgt ggcgacgagg tagatcttga ggcgggcggc
     5281 cgggaagacc acggtgccca tgtcccgccc gtcggcaacc aggcccgggg ggcgggcgaa
     5341 ggcgcgctgg cgatcgagca gtgcatcccg cacggcgggc caggcagcca ccctggaggc
     5401 cccctgcccg gcctcttccg agcggatctc gcgcgtgaca tcgacgccgt cgacccgcac
     5461 ccgctcgcca ccctgcccgt cgctgccgaa tacgacgtcc atcgaacggg caagcgccgc
     5521 atggccttcg acgtcctcgg cggagagccc cctgcgggcg cccagcaggc cgacgatgcg
     5581 gtacagggcg ccgctgtcga gcaggtgcca acccaccact cgggcgacct cacggctgac
     5641 cgtacccttg cctgagccgc tcgggccgtc gatcgcgact acgggaacgg cgtccatgcc
     5701 tgcatcctac gtaatgagga gtcccgcggc gcgcgccagt ggcacgaagc cgggaaagga
     5761 ggtggcgaca ttggccacgt ccaggatctc gagcggcccc gatgcccgca ggctcgcgac
     5821 cgcaaaggcc atggcgatgc ggtggtcacc gtggctgtcg acagtgccac cggacatcgg
     5881 cccgccttcg atgcggatgc cgtcgggcag cagttcgtgc accacgccga gccgcccgag
     5941 gccctcggcc attaccgcga gccggtcggt ctccttcacg cgcagctccc cggcgccacg
     6001 cagctcggtg acaccctcgg ccgcagcggc cgcgatgaac agcaccggaa actcatcgat
     6061 ggcgagcggc accagcgcct cgggaatagc gacaccccgc agcggtgcgg ccttgatgcg
     6121 caggtcggcg ctcggttcac cgccgctcac gcgggcgccc tccacgctga tatccgcgcc
     6181 catcagacgc aggatgtcga tcacgcccgt gcgggtggga ttcatgccga cactccgcag
     6241 caggaggcct tcatgcgcgg cgagacagcc ggcaacgatg aaaaaggccg ccgaggagat
     6301 gtcggccggc acgtcgaccc gcgtggcggt gagccgctgt ccgccctcga tggcaacggt
     6361 ggcgccgtca cgttgcacgc gcacgccgaa gccctgcagc atccgctcgg tgtggtcgcg
     6421 ggtcggcgcc ggctcggtga cgcgggtggt gcccgcagcc tgcaggcccg cgagcaggat
     6481 cgcggacttc acctgcgcgc tcggcaccgg caggtcgtaa tcgatagcgt gcagtctgcg
     6541 cccgccggtg atgcgcaccg gtggtctgcc atcgtgtgtc tcgatggcgg cacccatctc
     6601 gcgtagcggt ctcgcgacgc gctccatcgg ccgcgtcatc agcgaggcat cgccggtcag
     6661 cgtggtatcg aacgcctgcc ccgcgagcag ccccatgaaa agtcgcatcg ccgtgccgga
     6721 gttgccgaca tcgagtacct gacctggtgc ggacaggccg cgcacgccgg cgccgtgcac
     6781 gcagacccgg gtcggctccg tccggtcgac cgtgacgccc atggcctgca gcgcggcgag
     6841 cgtggcgagg cagtcctcgc tcggcaggaa accgtgtatc tcggtgcgac catcggcaat
     6901 gccgccaagc atcagcgcac gatgcgaaat ggacttgtca cccgggactg tgagttcgcc
     6961 gccgacgcgt tcgccgggct gcacgaggta gcggacctgc cggttcaacg cgcgccgtcc
     7021 cccggccagg catcgatgag ttcgagcagg cgcgcattct cctgcgggcg tccgatcgtg
     7081 atgcgcaggt attcggccag gccgtaggtg ccgaccgggc gcacgatgac gccaccgcgc
     7141 agcaggtgat cgttgagcgc cgcggcgcgc ggcgcgctgc cgatgtgcgc gagcaggaag
     7201 ttgccggccg atggcagcac cgtgaagcct cgcgaggcga gttcgcgcgc aaggcgcgga
     7261 cgctccgcct gcaccagcgc caccgagcgc acaaggtgct cgtcgtcggc gagcgccgca
     7321 cgcgcgccct cgagcgcaat cgaattcacg ttgaagggct ggcggatgcg gttcagcacg
     7381 tcggcgacct cgggatggct cgccgcatag cccacgcgca gccccgcgag accatatgcc
     7441 ttggagaagg tacgcacaac gacgagccgc gggtggcgcg cgagccaggc gatgccgtcg
     7501 ccgcaatcct cggcgcgcgc atattcgaaa tacgcctcgt cgagcacgac gagggtgctc
     7561 gacggcaggc ccgcgacgaa agcctcgagc gccgccgccc cgacccaggt gccggtgggg
     7621 ttgttggggt tcgcgacgaa caccaggcgt gtttgcgccg tgaccgccgc gcgcatcgca
     7681 tcgaggtcgt ggccgagggg cattcgcggg tgatccgccg gcagcgccgg caccacgatc
     7741 gcacgcgcgc cggtcgcctg cacggcgagc ggatagaccg cgaaacagta ttgcgagaat
     7801 accgcatcgg tgccgggggt gaggaacgcc tcggcgagca gcacgagcac gtcattagaa
     7861 ccattgccga gcgtgagctg cgccggcgcg acgccgagcc gcgcggcaag cgcctgcttc
     7921 agcgcgtggc cgctgccatc gggataggcg gccgactccg cgagcgccgc ctgcattgcg
     7981 gccatcgccg cggggcccgc accgagcgga ttctcgttcg atgcgagctt gatgatgtcg
     8041 gtgagcccga gctcgcgcgc aacttcgctg accggcttgc cgggcgtgta cggtgcgatc
     8101 tgccgcacgc actcgctcgc cagccgggcg ggatgctcgg tgctcacagg atcgcccgcg
     8161 ggtaggcgcc gagcacgcgg aacagcgagg cgcgcttctt cagactggcg agcgccttgg
     8221 cgatggccgg gtcgtccgcg tgcccctcga tgtcgagaaa gaacacgtag tcccacttgc
     8281 ggcgccccga cgggcgcgac tcgatgcgcg tgacgttgat gcgatgcctc gcgagcggct
     8341 cgagcagccg gtagagcgcg cccggtgcat cggtatgccc gcccgagacg atgaaggtgg
     8401 tgcgatcgac gccgctcggg gcgaacagct tgcggccgat caccaggaag cgggtcgtat
     8461 tgtcgggccg gtcctcgatc tgcgccgcga ggatgttgag tccgtacact tcggccgccg
     8521 tgtcgccggc gatcgccgcg gtaccctgct cgtcgcgcgc ccggcgcgcg ccctcggcat
     8581 tgctcgccac cggcacgagt tcgatgtccg ggagattctc gcgcagccag tcgcggcact
     8641 gcgccagcga ctgcggatgc gagcagatgc gcttgacctt gcgcaggctg tccatgcgcc
     8701 ccatcaggaa atggtggatg cgcagctcca cctcaccgca gatgtgcagc ggcgatgaga
     8761 ggaatcggtc gagcgtgtag ttgacggtgc cctcgctcga gttctcgatc ggcacgacgc
     8821 cgaagtcggc actggccgcc tccacctcgt ggaacacctc ctcgatcgaa gcgagcgcga
     8881 gcgcgcgcac cgaatgcccg aagtgcttga ggacggcagc ctgcgtgaac gtgccctcgg
     8941 gaccgaggaa ggcgaccttg agcggctcct gctgcgcgag gcacgccgac atgatctccc
     9001 ggaacagccg cagcacttcc tcgtcgcgca gcggcccctt gttgcgctcg agcgcgaggc
     9061 gcaggacctg cgcttcgcgc tccggccggt agaaatcgac ggtgtggccg tctttgtgct
     9121 tcgagatgcc aacctgctgc gcgagcgcgg cgcgctcgtt cagcaggtca tggatgcgcg
     9181 catcgacgtc gtctatgcgc cggcgcacgt catcgagcgc caccctgccg ggcgcactcc
     9241 gcttcgcctt ccccttcgcc ctcgccatcg gcttcggctt cggcttcggc ttcgctgttg
     9301 ccttgcgtcc cgccatggcg gcctgccgcg cgtcacacga cgcgagcgga cagcaccccg
     9361 tggatggcgc ggagccgctc gacgacacct gccggcacct cgccgtcggc atcgatcagc
     9421 gtgtacgcat actcgccgcg cgacttgttg agcaggtcgg caatgttgag cttggcctcg
     9481 gcgaggcagt tcgagatctg gccgatcatg gtcggcacgt tgctgttggc gatcgcaata
     9541 cgcgaggtgc ccggcacacg cgacagcacc gcttcgggga aattgacgca gttgcgcagg
     9601 ttgccgttct cgaggaaatc gcgcacggtc tcggcgacca tcaccgcgca gttctcctcg
     9661 gcttcgccgg tcgaggcgcc gaggtgcggc agcgtgacga tgcgcggatg gtccttgagg
     9721 gcgttcttcg ggaaatcgca cacgtaggcg cgcaggtgcc cggcatccag cgaggcgatg
     9781 atcgccttgt cgtcgacgat cggcgcgcgc gcgaaattga ggaccacgcc acccttgcgc
     9841 atcagcgcga gacgggcctc gttgaccagg ccccgggtct ggtcattgag cggcacatgc
     9901 accgtcacga tatcggcgcg tgagaacagg tcgtcgagcg acagcgcctg ctcgacattc
     9961 gaggagagct gccaggcgcg ttgcacggtg atctgcgggt caaagcccag caccttcatg
    10021 ccgagagcga gcgaggagtt cgccacctcg acgccgatgg caccaaggcc cacgacgccg
    10081 agggtgcgcc ccggcagttc gaaaccgacg aagttcttct tgcccttttc gaccgcgacg
    10141 tcgatttcct tgtcgtcgcc ggtcacgctg cgtgcgaagt cccaggcggc gcacacattg
    10201 cgcgccgcga tcagcatgcc ggcaatcacc agttccttga ccgcgttggc attggcgccc
    10261 ggcgcgttga acaccggcac accgcgctgc gacagcgccg ccaccgggat gttgttgacg
    10321 cccgcgccgg cgcgccccac ggcgagcagg ctgtcgggga gctgcagcgc gtgcatgtcg
    10381 gccgagcgca cgaggatggc atcgggcctg gcgatatccg aggcgatttc gtagcgctcg
    10441 cgcggcagcc gctcgaggcc tttgacactg atgttgttga gagtctggat cctgaaagtc
    10501 atggtcgatg gcagcgccgg cgttcagccg tggcgccgct ggaactcct
//
LOCUS       DAOQVY010000079        10486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_7114_length_10486_cov_5.631387, whole genome shotgun sequence.
ACCESSION   DAOQVY010000079 DAOQVY010000000
VERSION     DAOQVY010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10486
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7114_length_10486_cov_5.631387"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1691
                     /gene="msbA"
                     /locus_tag="PKN91_08315"
     CDS             <1..1691
                     /gene="msbA"
                     /locus_tag="PKN91_08315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HNS28115.1"
                     /translation="ARPYVGTFLIGVCGMVLFAATDALLAYLVKIFLGGAFIERDPRI
                     IWIVPLGAVTLFLLRGLGDYVSTYFPAWVGRQIIKALRRELFAKYLRLPVSWFDATST
                     GEALSRLTFNIELVAEATTNAVTVMIRDTLTIMALLGWLFWLNWQLAAFALVLAPLIS
                     WLIRAINRSFRRYSTRIQNSMADVNRVAKESLDGQRVIRVFNAQRQLEQSFEVANELN
                     RHSNMRLVNARAMSNPVVQFVAALGLAGVLYFAISQVQAHAMRVDDFMSFLTALLLIT
                     APLRRLVNIFVPLQQGIAAGASIFAVLDSPDEDQGGTLRVERARGDIAYRDVSFAYRT
                     DKGQVLEHVDLEVRAGRTLAIVGRSGSGKSTLVALLPRFYDVDSGSVQLDGRDVREYA
                     LDSLRDQVAVVSQDVVLFNDTIRANIAFGQPGATAAQVEAAAEAAFVMDFVADLPQGL
                     DTLVGDRGALLSGGQRQRISIARAILKDAPVLVLDEATSALDTESERHIQAALGRLRS
                     GRTTLVIAHRLSTVEQADEIVVMDEGRIVERGTHAALLAQDGIYAQLRRLQFNA"
     gene            1707..2717
                     /gene="lpxK"
                     /locus_tag="PKN91_08320"
     CDS             1707..2717
                     /gene="lpxK"
                     /locus_tag="PKN91_08320"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNS28116.1"
                     /translation="MEQWVNSIWYGRSFVAVLLRPLSWLYALVVGARRRAYAWGLFST
                     TKLPVPVVVVGNLTVGGTGKTPFVVWLVAQLAAAGHKPGVISRGYGRRGSAPRLVDVD
                     SSAGEAGDEPLLIRRRTERPVAVGSDRVVAGRLLGTQSVDIIVADDGLQHLALARDCE
                     FVLIDGARGFGNGLMLPAGPLREPAGRLRHVDAVVMVDGSNERSLAGRPVFQMRLVAE
                     HIVPLAGPQDAKLALASLAGQRVHAVAGIGHPERFFRTLRAAGLDVVPHAFPDHHDYT
                     SADLAFDDDLPILMTEKDAVKCTAFASSRTGYVPVSAAFSEADASALLALISTRAFAS
                     SR"
     gene            2727..2915
                     /locus_tag="PKN91_08325"
     CDS             2727..2915
                     /locus_tag="PKN91_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008375064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNS28117.1"
                     /translation="MDTRLLDIIACPLCKGPVVHRRAEAVLVCRGDRLAYPIRDGVPI
                     MLVEEAKPLAVDDPLLER"
     gene            2923..3648
                     /gene="kdsB"
                     /locus_tag="PKN91_08330"
     CDS             2923..3648
                     /gene="kdsB"
                     /locus_tag="PKN91_08330"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNS28118.1"
                     /translation="MFRVVIPARYQSSRFPGKALALIDGQPMIVRVHERARLTGAAEV
                     IVATDDERIAAAVRAAGADVCMTAASHASGTDRIAEVARLRGFAGEDIIVNLQGDEPR
                     MPAAIVDQVAAALVEHAGADIATAAYPVQGRADYLDPNVVKVVTDGAGRALYFSRAPI
                     PWNRDAAGAADAHRGARRHIGLYAYRTAALLRLAALPPTPLERLEQLEQLRALEHGME
                     IRVVDAAEPPGADVNTPEDLLRT"
     gene            3669..4625
                     /gene="rarD"
                     /locus_tag="PKN91_08335"
     CDS             3669..4625
                     /gene="rarD"
                     /locus_tag="PKN91_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HNS28119.1"
                     /translation="MPPQEAPAASRSSIDRRGLAAAIGAYAMWGLFPLYWYQLKTVPA
                     VQTVAHRVVWCAVFVLGWVALRHERGWLRRALAAPRVLPLLAASSALISLNWGIYIWA
                     VTHGRVLDTSLGYFINPLANVLVGVLLLRERLRATQWAAVGLGALGVLWLTFRHGELP
                     WVALALAATFCLYGLIRKVVAVGAIPGLAIENLILLLPALVWLGWSDLRGEGVFGHGG
                     GPPELLLIVGGALTAVPLIGFAYGARRLSYSLVGLLLYVSPTLQLACGLLLGEAFTQD
                     KMIGFACIWLGLAVYAVDGWRGSRNRAPLATAARRDPGDNRA"
     gene            complement(4635..5186)
                     /locus_tag="PKN91_08340"
     CDS             complement(4635..5186)
                     /locus_tag="PKN91_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28120.1"
                     /translation="MHTVMRLAACGVLLTLAACDRGPTGSVPEAAAPAADDAALPAVV
                     AAALAEIAAECSDVGGTPHTRDAVRRIDLNGDGRDDYVLHTGWVMCENAVSVYGDRAK
                     RLAIFAGDGRGGAAESFSDWTYDAQFEGDPATLWLVVAGDNCGQPPAPDFASESFCQR
                     AITWNSGSGRFDYAPVDTVRMIE"
     gene            complement(5293..5778)
                     /locus_tag="PKN91_08345"
     CDS             complement(5293..5778)
                     /locus_tag="PKN91_08345"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HNS28121.1"
                     /translation="MDPALTRDSLERLVGEQLATLTRLEELLEREHALLVDRDVEQLA
                     AAGRDRQACVGALLRIDSERAALCRMFGHAADPAGMQKLLASCDASGRLATRWQSCTE
                     MTRRCRKLNDRNGALATAQLRSVSGRLDALTGGAPRSAATYAPGGTARPSTGRLLAAN
                     A"
     gene            complement(5786..6097)
                     /gene="flgM"
                     /locus_tag="PKN91_08350"
     CDS             complement(5786..6097)
                     /gene="flgM"
                     /locus_tag="PKN91_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNS28122.1"
                     /translation="MDPLVPKPSEVGAARGARAAGHGDAGARAQAGTAGIQITEGARQ
                     LAALEATIRRLPQVDEARVARIRKAIEDGSYTVDAEATATKLMVFEHDLESLARSVAR
                     D"
     gene            complement(6206..6928)
                     /gene="flgA"
                     /locus_tag="PKN91_08355"
     CDS             complement(6206..6928)
                     /gene="flgA"
                     /locus_tag="PKN91_08355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNS28123.1"
                     /translation="MRTRITRGLLRHARYAVLLAAVPLVAQAQAIEDPADIGAAASAF
                     LRAQVPASPGVTTVIHAAHLDPRLRLPKCGVPLQATWAPGSQPAARTNVFVACPQGKP
                     WRVNVPLTLRSRLDVLVLTAAAARGRTLAATDVATRNVEVDGLSHSYIRGPAEIAGKH
                     LARPAPAGAPLQAAWFTADKLVKRGQQVTLVALADGIRIRAPGRALSDGAARERVRVQ
                     NLSSAKIVEGVVENATEVRVTP"
     gene            7146..7547
                     /gene="flgB"
                     /locus_tag="PKN91_08360"
     CDS             7146..7547
                     /gene="flgB"
                     /locus_tag="PKN91_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNS28124.1"
                     /translation="MRFNLDAYLGVHQQALRLGAKRTEVIANNLANANTPGFKARDVD
                     FRAALAAAGDGGSLPMAQTRPAHLPTGAADGDVAGALRYRTPLAPSLDGNTVDPQLEQ
                     AAFAENAVRYQATLAFLNARFRGLMTAITGQ"
     gene            7577..8005
                     /gene="flgC"
                     /locus_tag="PKN91_08365"
     CDS             7577..8005
                     /gene="flgC"
                     /locus_tag="PKN91_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNS28125.1"
                     /translation="MSSFKTFDIAGSGMAAQSVRLNTVASNLANADSVSGDAAAVYKA
                     RHPLFEAVRQQLAGASPAAQGGSAVRVTGIVESEAAPVARYEPGNPLANADGYVFAPN
                     VNVVEEMVDMISASRSYQNNVEVMDTAKGMMLATLRLGQS"
     gene            8002..8685
                     /locus_tag="PKN91_08370"
     CDS             8002..8685
                     /locus_tag="PKN91_08370"
                     /inference="COORDINATES: protein motif:HMM:NF025230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HNS28126.1"
                     /translation="MSIVSNDAANPYAGLGFSTTASGIAAAGGDALDQQDFLMLLTTQ
                     LKNQDPLKPLDSTQFVSQLAQFSQVSGVQEMNASLHSLTSSMKSSAVLDGAALVGRYV
                     LLEGDTATLAAGSSIVGGIATPAGATAITVNVRSAGGELVQSMQVAPTEGTTLFSWDG
                     VQRDGTIAAPGIYRFDAMASNGSRTEAAPVMVADLVASVSIDAATYALQLNTATSGPV
                     TLAEVRQVF"
     gene            8720..9931
                     /gene="flgE"
                     /locus_tag="PKN91_08375"
     CDS             8720..9931
                     /gene="flgE"
                     /locus_tag="PKN91_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HNS28127.1"
                     /translation="MPFTIALSGLNAASADLAVTANNIANGSTTGFKSSRTEFADLFA
                     VTPFGVASTATGNGVKVSSVTQQFTQGNIDFTDSNLDLALSGDGFFTLSDGGALAYTR
                     SGAFKVDNQGYVVSPTGSRLQVYPPLANGGFNTGGLSDLQLVTNESAPQATSEIRMAL
                     NLPADATAPVSAFDPADATSYNRQTSMTVYDSLGAAHTNSFYFVKGAAANEWTVQAYT
                     DGAPVGTPQPLTFDSNGALVTPAGGSIAFPPHVPATGAQPLAITLDVRGATQYGSNFG
                     VQYTQDGYTTGALSGIDVDATGVVSARYTNGRSIPLGQVAVTNFANVQGLQKLGDTQW
                     GETFGSGAAQRGTAGSSGFGVIQSGALEASNVELTEQLVNMIIAQRNFQANAQMISTA
                     DQVTQTVINIR"
     gene            9950..>10486
                     /gene="flgF"
                     /locus_tag="PKN91_08380"
     CDS             9950..>10486
                     /gene="flgF"
                     /locus_tag="PKN91_08380"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HNS28128.1"
                     /translation="MDKYLYVAMSGAKETLRAQAVNSHNLANAGTTGFRADLAAFQTR
                     IVEGSGHASRAYATHAAVGWNGASGALLGTGRDLDVAVQGEGWIAVQAADGSEAYTRA
                     GDLRVDTTGLLRNGAGDVVLGDGGPLTVPQHSSISVAADGSVSIVPIGQGPETTAIVG
                     RIKLVNPAPDTLVRGGDGL"
BASE COUNT         1570 a         3555 c         3632 g         1729 t
ORIGIN      
        1 acgcgcggcc ctacgtcggc actttcctga tcggcgtctg cggcatggtg ctgttcgccg
       61 ccaccgacgc gctgctcgcc tatctggtca agatcttcct cggcggtgcg ttcatcgaac
      121 gcgatccgcg catcatctgg atcgtgccgc tcggcgccgt gaccctcttc ctgctgcgcg
      181 gcctcggcga ctatgtctcg acctatttcc cggcctgggt cgggaggcag atcatcaagg
      241 cgttgcgtcg cgagctgttc gccaagtatc tgcgcctgcc ggtgtcgtgg ttcgatgcga
      301 cctcgaccgg cgaggcgctc tcgcggctca ccttcaacat cgagctggtg gccgaggcga
      361 ccaccaacgc cgtcacggtg atgatccgcg acacgttgac gatcatggcg ctgctcggct
      421 ggctgttctg gctcaactgg caactggccg cgttcgcact ggtgctcgcg ccgctcatct
      481 cgtggctgat ccgggccatc aaccgctcct tccgccgcta cagtacgcgc atccagaatt
      541 cgatggcgga cgtgaaccgc gttgcgaagg agtcgctcga cggccagcgc gtcatcaggg
      601 tgttcaatgc gcagcgccag ctcgaacaga gtttcgaggt ggcgaacgag ctgaaccgcc
      661 acagcaacat gcgcctggtc aatgcgcgcg cgatgagcaa tccggtagta cagttcgtgg
      721 cggcgctcgg gctcgccggc gtgctgtact tcgccatcag ccaggtgcag gcgcatgcca
      781 tgcgggtcga cgacttcatg tcgttcctga ccgcgctgct gctgatcacg gcgccgctgc
      841 gccgcctcgt gaatatcttc gtaccgctgc aacagggcat cgcggccggt gcgagcatct
      901 tcgcggtgct cgacagcccg gacgaggacc agggtgggac gctgcgggtc gagcgcgcgc
      961 gcggcgacat cgcctaccgc gacgtgagct tcgcgtaccg gacggacaag ggacaggtgc
     1021 tcgagcacgt cgatctcgag gtgcgcgccg gacgcacgct cgccatcgtc gggcgctcgg
     1081 gcagcggcaa gtcgacgctg gtcgcgctgc tgccgcgctt ctacgacgtc gactcgggca
     1141 gtgtgcagct cgacggccgc gacgtgcgcg agtacgcgct cgattcgttg cgtgaccagg
     1201 tcgcggtagt gagccaggac gtcgtgctgt tcaacgacac gatccgggcg aacatcgcct
     1261 tcggccagcc tggcgccacc gcggcgcagg tcgaggcggc ggcggaggcc gcgttcgtga
     1321 tggacttcgt ggccgacctg ccgcagggcc tcgatacgct ggtgggcgat cgcggcgcgc
     1381 tgctctccgg cggccagcgc cagcgcatct cgattgcacg cgcgatcctg aaggacgcac
     1441 cggtgctcgt gctcgatgag gccacctcgg cgctcgacac cgaatccgag cgtcacatcc
     1501 aggcggcgct cggccgcctg cgctcggggc gcaccacgct ggtcatcgcg caccggctgt
     1561 cgaccgtcga gcaggccgac gaaatcgtcg tgatggacga gggtcgcatc gtcgagcgcg
     1621 gcacccatgc cgcgctgctc gcgcaggatg gcatctacgc gcagctgcgt cgcctgcagt
     1681 tcaacgcctg aggccggacg ggcgccatgg agcagtgggt caactcgatc tggtatgggc
     1741 gctcattcgt ggccgtgctg ctgcgcccgc tgtcgtggct ctatgccctg gtcgtcggag
     1801 cgcgacgcag ggcctacgca tgggggctgt tcagcaccac gaagttgccg gtgccggtcg
     1861 tggtcgtcgg caacctgact gtcggcggca ccggaaaaac gccgtttgtc gtatggctgg
     1921 tcgcgcaact cgcggccgcg gggcacaagc cgggggtgat ctcgcgcggc tatggtcgcc
     1981 gcggctccgc cccgcggctc gtcgacgtgg actcgagcgc cggcgaggcc ggggatgagc
     2041 cgctgctgat ccgccggcgc accgagcggc cggtcgccgt cggcagcgat cgtgtggtgg
     2101 cgggccggct gctcggcacg cagtcggtgg acatcatcgt ggcagatgac ggcctgcagc
     2161 acctggccct ggcgcgcgac tgcgagttcg tgctgatcga tggtgcgcgc ggcttcggca
     2221 acggactgat gctgccggcc ggtcccttgc gcgagcccgc cgggaggctg cgccacgtcg
     2281 acgccgtagt catggtcgat ggctcgaacg agcgcagtct ggccgggcgg cccgtgttcc
     2341 agatgcggct cgtcgccgag cacatcgtgc cgctcgcggg cccgcaggat gcgaagctcg
     2401 cgctcgcgag cctcgccggc cagcgcgtgc atgcggttgc cggcatcggc catcccgagc
     2461 ggttcttccg cacgctgcgc gccgccgggc tcgatgtggt cccgcatgct ttccccgatc
     2521 accacgacta cacgtcggcg gacctcgcct tcgacgacga cctgccgatc ctcatgaccg
     2581 agaaggatgc cgtaaaatgc acggcgttcg cgtcatcgag gaccgggtat gtgccggtca
     2641 gtgccgcgtt cagcgaggca gacgccagcg cgctgctggc gctgatctcg accagggctt
     2701 tcgcctcttc acgctgagga tccgcattgg acacccgatt gctcgatatc atcgcctgcc
     2761 cgctctgcaa aggacccgtc gtgcatcgcc gcgcggaagc agtactcgtg tgccgcggcg
     2821 accggctcgc ctatccgatc cgtgacggcg tgccgatcat gctggtcgag gaggcgaagc
     2881 cgctcgcggt cgacgacccg ctgctcgaac gctgaagccg gcatgttccg cgtcgtcatc
     2941 ccggcccgct accagtcgtc gcgctttccg ggcaaggcgc tcgcgctgat cgacggacag
     3001 ccgatgatcg tgcgcgtgca cgagcgcgcc cggctgaccg gcgccgccga ggtgatcgtc
     3061 gcgacggatg acgagcgcat cgcggccgcg gtgcgcgcgg cgggtgccga cgtctgcatg
     3121 accgcggcgt cccatgcgtc gggtaccgat cgcattgccg aggtcgcacg cctgcggggc
     3181 tttgccggtg aggacatcat cgtgaacctg cagggtgacg aaccgcgcat gcccgcggcg
     3241 atcgtcgacc aggttgccgc cgcgctggtg gagcatgcgg gcgcggatat tgccaccgcg
     3301 gcgtacccgg tgcagggtcg cgcggactat ctcgatccga atgtcgtgaa ggtcgtcacg
     3361 gacggcgcgg gcagggcgct ctatttcagt cgcgcgccga ttccgtggaa tcgcgacgcg
     3421 gcgggagccg ccgatgccca tcgcggtgca cggcgccata tcggcctcta tgcctaccgc
     3481 acggccgcgc tgctgcgcct cgcggcgctg ccgccgacac cgctcgagcg gctcgagcag
     3541 ctcgagcaac tgcgtgcact ggagcacggc atggagatcc gcgtggtcga cgcggcggaa
     3601 ccgccgggtg ccgacgtcaa tacaccggag gatctcctgc ggacataaag ctccctggcg
     3661 ggcagggtgt gccgccgcaa gaggcaccgg ccgcgtccag gtcgtccatc gaccgtcgcg
     3721 gactcgccgc ggcaatcggt gcgtacgcca tgtggggcct gttcccgctc tactggtatc
     3781 agctgaagac ggtaccggcg gtgcagaccg tcgcccaccg ggtggtgtgg tgcgccgtct
     3841 tcgtcctcgg ctgggtcgcg ctgcgccacg agcgtggctg gttgcgacgt gcgctcgccg
     3901 cgccgcgcgt gctgccgctg ctcgccgcga gcagcgcgct gatcagcctc aactggggca
     3961 tctatatctg ggcggtgacg catggccgcg tgctcgatac gagcctcggc tacttcatca
     4021 acccactggc gaacgtgctg gtcggcgtac tgctgctgcg cgagcgcctg cgcgcgacgc
     4081 aatgggccgc ggtgggcctc ggcgcactcg gcgtgctgtg gctcaccttc cgtcatggcg
     4141 agttgccctg ggtggcactc gcgctggcgg cgactttctg cctctatggg ctcattcgca
     4201 aggtcgtcgc agtcggcgcg attccggggc tcgccatcga gaatctgatc ctgctgttgc
     4261 ccgcgctcgt ctggctcggc tggagcgatc tgcgaggcga gggggtcttc ggtcatggcg
     4321 gcgggccgcc cgagctgctg ttgatcgtcg ggggcgctct gaccgcggtg ccactgatcg
     4381 gcttcgccta tggcgcgcgc cggctgtcct attcgctggt cggtctgctg ctgtatgttt
     4441 ccccgacact gcaactcgcg tgcgggctgc tgctgggaga ggcgttcacg caggacaaga
     4501 tgatcggctt cgcgtgcatc tggctcggcc tcgcggtcta tgcggtggac ggatggcgcg
     4561 gcagccgcaa tcgtgcgccg ctcgcgactg cggcccgccg cgatccgggt gacaaccggg
     4621 cgtgatgccg gcgcctactc gatcatgcgc accgtatcga cgggcgcgta gtcgaaccgg
     4681 ccggagccgc tgttccaggt gatcgcgcgc tggcagaagg actcgctcgc gaagtccggt
     4741 gcgggcggtt gcccgcagtt gtcgccggcc acgacgagcc acagcgtggc cggatcacct
     4801 tcgaactgcg cgtcgtaggt ccagtccgaa aaggattcgg ccgcgccgcc ccggccatcg
     4861 cccgcgaaaa tggcgagacg cttggcgcga tcgccataga cgctcacggc gttctcgcac
     4921 atcacccagc ccgtatgcag cacgtagtcg tcgcggccgt cgccattcag gtcgatgcgc
     4981 cgaaccgcat cccgggtgtg cggcgtgcca ccgacgtcac tgcattccgc ggcgatctcc
     5041 gccagtgcgg cggcgacgac cgcaggcaat gcggcgtcat ccgccgcggg ggccgcagct
     5101 tccggcacgg atccggtcgg tcccctgtcg caagccgcca gcgtgagcag aacaccgcag
     5161 gccgcaagtc gcatgactgt gtgcatgaac gccccctttg cagtcgctca ggcgccgagc
     5221 tttgcgttca tgttcatgcg ggccttcgac tgtcacgata ccgggcagtt ccggggagca
     5281 tacacctgcg cgtcaggcgt tggcggcgag cagcctgccg gtggacgggc gggcagtacc
     5341 gcccggggcg taggtcgcgg cgctgcgcgg agcgccgccc gtgagcgcgt cgagccggcc
     5401 ggagacgctg cgcagctgcg cggtagcaag tgcaccgttg cgatcgttga gcttgcggca
     5461 acgccgcgtc atttcggtac atgactgcca gcgggtcgcg aggcgaccgc tcgcgtcgca
     5521 ggaggccagg agcttctgca tgccggcggg atcggcggca tggccgaaca tgcggcatag
     5581 tgcggcacgc tcactgtcga tccgcagcag cgcaccgacg caggcctgcc ggtcgcgtcc
     5641 tgcagccgcg agctgttcga catcacgatc gaccagcagc gcgtgctcgc gttccagcag
     5701 ttcctcgagt cgcgtgagcg tcgccagctg ttcgccgacg agtcgttcca ggctgtcgcg
     5761 ggtcaatgcc ggatccatgc gctcgtcagt cccttgcgac ggagcgcgcg aggctctcga
     5821 gatcatgctc gaagaccatc agcttggtcg cggtagcctc ggcgtcgacg gtgtagctgc
     5881 cgtcctcgat cgccttgcgg atcctggcga cccgggcctc gtccacctgg gggagcctgc
     5941 ggatcgtggc ttcgagtgcc gcgagctggc gcgcaccctc ggtgatctgg atgccggcgg
     6001 tgcctgcctg cgcgcgcgca ccggcatcgc cgtggcctgc agcgcgcgca cccctcgcgg
     6061 caccaacctc agatggcttg ggaacaaggg ggtccactcc attgattttg ctagccatct
     6121 tgggctctcc catgcgtgtc ttgccttata acggcccgcc ggacggaaac tttaggcccg
     6181 cgaggccccg gggcgacagg gtcaatcatg gcgtcacgcg tacttcggtg gcgttctcga
     6241 ccacgccctc gacgattttc gccgacgaca ggttctgtac ccgcacccgc tcacgggcgg
     6301 caccgtcgct cagggcacgc cccggtgcgc gaatccggat gccgtcggcg agcgccacca
     6361 gcgtgacctg ttggccgcgc ttcaccagtt tgtcggcggt gaaccacgcg gcctgcaggg
     6421 gcgcgccggc gggggcgggt cgggcgaggt gctttccggc gatctccgcc gggcctctta
     6481 tataggagtg cgacaggccg tcgacttcga cgttgcgggt ggcgacatcc gtggccgcga
     6541 gcgtgcgtcc gcgcgccgcc gccgcggtga gcacgagcac gtcgagccgg ctgcgcaatg
     6601 tcagcggcac gttcacgcgc caaggcttgc cctgcggaca ggcgacgaaa acgttggtgc
     6661 gcgcggccgg ttgcgagccc ggcgcccacg tggcctgcag cggtacgccg catttgggga
     6721 gcctgaggcg cgggtcgagg tgcgcggcgt ggatcacggt cgtgacgccc ggactcgcag
     6781 ggacttgtgc gcgcaggaag gcggaggccg ccgcgccgat gtccgcgggg tcctcgatgg
     6841 cctgcgcctg ggcgacgagc ggtacggcgg cgagcagtac cgcgtagcgc gcatggcgca
     6901 gcaagccgcg agtgatccgg gtcctcatgc tgcaggggac tgcagattgc atgccagcgc
     6961 gggcctgcgc gcagcggagc cagccgttgc cgccggcgcg gcaaggcttt gccgccgcag
     7021 cgtgacgcgt gtcacatcgg gcgggcgatg gcgatcgcac atcgcccgtg aagtgccaat
     7081 ggctgccacg tggcacaggg tttgctgtgc tcctgagcag tcaggtttct gacggtggga
     7141 acgacatgcg attcaatctt gacgcttacc tcggtgtgca tcagcaggcg ctgcggctgg
     7201 gtgcgaagcg caccgaagtg attgcgaaca atctcgccaa cgccaacacg ccgggcttca
     7261 aggcccgtga tgtcgatttt cgcgcggcgc tggccgcggc cggcgacggc ggatcgctcc
     7321 cgatggcgca gacgcgaccc gcacatctcc cgaccggcgc cgcggacggc gatgtggccg
     7381 gggcacttcg ttatcgcacg cccctcgccc cgtccctcga cggcaacacg gtcgacccgc
     7441 agcttgagca ggcggcgttc gccgagaacg cggtccgcta ccaggccacg ctcgcttttc
     7501 tcaatgcgcg ctttcgcggc ctgatgaccg cgatcacggg ccagtgatcg cggcgcactg
     7561 aaacaggaga cgcacgatgt cctcgttcaa gactttcgat atcgccggat cgggcatggc
     7621 cgcccagtcc gtgcgcctga acaccgtggc gagcaacctc gccaacgccg acagcgtcag
     7681 cggcgatgcg gcggccgtgt acaaggcccg ccatccgctg ttcgaagccg tccgccagca
     7741 actcgccggc gcgagcccgg ccgcgcaggg cgggtccgcg gtgcgagtca ccggcatcgt
     7801 cgaaagcgaa gccgcgccgg tcgcgcgtta cgagcccggc aatccgcttg cgaatgccga
     7861 cggctacgtc ttcgccccga acgtcaacgt cgtcgaggaa atggtcgaca tgatctcggc
     7921 gtcccgctcc taccagaaca acgtcgaagt gatggatacg gccaagggca tgatgctcgc
     7981 gacgctgagg ctcgggcagt catgagcatc gtaagcaacg atgccgccaa tccatatgcg
     8041 ggcctcggct tcagcaccac cgcctcgggc atcgccgccg ccggcggcga tgcgctcgac
     8101 cagcaggact ttctgatgct gctgacgacc cagctgaaga accaggatcc gctgaaaccg
     8161 ctcgactcga cgcagttcgt gagccagctc gcgcagttca gccaggtgtc cggcgtgcag
     8221 gaaatgaatg cgtcgctgca ctcgctcacg agcagcatga agagttccgc ggtgctcgac
     8281 ggcgcggcgc tggtcggccg ttacgtgctg ctcgagggcg ataccgcgac gctcgcggcg
     8341 ggcagctcga tcgtcggcgg catcgcgacg ccggccggcg cgactgccat caccgtgaat
     8401 gtgcggagtg ctggcggcga actcgtgcaa tcgatgcagg tcgcgccgac cgaaggcacg
     8461 acactgttca gctgggatgg cgtgcagcgt gacggcacga tcgccgcgcc cggcatctat
     8521 cgcttcgatg cgatggcctc gaacggcagc cggacggagg ctgcgccggt catggtcgcg
     8581 gacctggtcg cgagcgtcag catcgacgcg gcgacctacg cattgcagct gaacacggcg
     8641 accagcggcc cggtgacact ggccgaagtc cgccaggtgt tctgaacccg aatccgaatc
     8701 gcgaaggagt acacgcaaca tgccatttac catcgccctc agcggcctga atgccgcctc
     8761 ggccgatctc gccgtgaccg cgaacaacat cgcgaacggc tcgaccaccg gcttcaagag
     8821 ctcgcgcacg gagttcgcgg acctgttcgc cgtcacgcct ttcggcgtgg cgagcacggc
     8881 caccggcaat ggtgtgaagg tctcgtcggt gacccagcag ttcacgcagg gcaatatcga
     8941 cttcaccgac agcaacctcg atctggcgct ctcgggcgac ggtttcttca cgctgagcga
     9001 cggtggtgcg ctcgcctaca cgcgctccgg tgcgttcaag gtggacaacc agggttacgt
     9061 cgtgagtccg acgggctcgc ggctgcaggt ctatccgccg ctggcaaacg gcggcttcaa
     9121 caccggcggc ctgtcggacc tccagctcgt gaccaacgag agcgcgccgc aggcgacctc
     9181 ggagatccgc atggcgctca acctgccggc cgatgccacc gcgccggtga gtgcgttcga
     9241 tccggcggat gcgaccagct acaaccgcca gacgtcgatg acggtctacg actcgctggg
     9301 cgcggcgcac accaacagct tctatttcgt caagggtgcc gcggccaacg aatggaccgt
     9361 gcaggcctac accgatggcg cgccggtcgg cacgccgcag ccgctcacct tcgacagcaa
     9421 cggtgcgctc gtgactccgg caggtggctc gatcgccttc cccccgcacg tgccggcgac
     9481 cggcgcgcag ccgctcgcga tcacgctcga cgtgcgtggt gcgacccagt acggcagtaa
     9541 tttcggcgtg cagtacaccc aggacggata caccaccggt gcgctcagcg gtatcgacgt
     9601 cgatgcgacc ggagtggtca gcgcacgcta caccaatgga cgctcgatcc cgctcggcca
     9661 ggtggccgtg acgaacttcg cgaacgtcca gggtctgcag aagctcggcg acacgcagtg
     9721 gggcgagacc ttcgggtccg gtgccgcgca gcgtggcact gcgggcagct cgggtttcgg
     9781 cgtgattcag tctggcgccc tcgaggcgtc gaacgtcgag ctcaccgaac agctcgtgaa
     9841 catgatcatc gcgcagcgca atttccaggc aaacgcacag atgatctcca ccgcggacca
     9901 ggtcacgcag accgtgatca atatccgctg agcgcaggtg tagcggcaga tggacaagta
     9961 cctgtacgtg gcgatgtccg gcgcgaagga aaccctgcgc gcgcaggccg tcaacagcca
    10021 caacctggcc aatgcgggca cgactggctt tcgcgcggac ctcgccgcgt tccagacgcg
    10081 catcgtggag ggcagcgggc acgcctcgcg tgcctatgcc acgcatgcgg cggtcggctg
    10141 gaacggcgcg agcggcgcgc tgctcggcac gggccgcgac ctcgacgtgg ccgtgcaggg
    10201 cgagggctgg atcgcggtgc aggcggccga tggcagcgag gcctatacgc gcgccggcga
    10261 cctgcgtgtc gacaccacgg gcctgctgcg caacggcgct ggcgacgtgg tgctcggcga
    10321 tggcgggccc ctcacggtgc cgcagcacag ttccatctcg gtggcggcgg atggttcggt
    10381 ctcgatcgtg ccgatcgggc agggaccgga aacgactgcc atcgtcgggc gcatcaagct
    10441 cgtcaatcct gcgccggaca cgctggtacg cggcggcgac ggtctc
//
LOCUS       DAOQVY010000080        10182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_7392_length_10182_cov_5.404957, whole genome shotgun sequence.
ACCESSION   DAOQVY010000080 DAOQVY010000000
VERSION     DAOQVY010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10182
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7392_length_10182_cov_5.404957"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PKN91_08385"
     CDS             complement(<1..285)
                     /locus_tag="PKN91_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28129.1"
                     /translation="MADDGQAFVAAVAIAASGYVMWRRYMRAASLMEGTATSRVATAA
                     KGYVELAGTARAAGGAPVRDPIQMQECLWFHVVTEKRGSFKNNSRWRVEKS"
     gene            complement(278..>758)
                     /locus_tag="PKN91_08390"
     CDS             complement(278..>758)
                     /locus_tag="PKN91_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNS28130.1"
                     /translation="VAVYNQLVLVKHNVTKAWANIDVLLKQRNDEIPKLVDVCRQYSR
                     FEAALLARLTGARAAAVAAQQGANAQSVGAAEQALRGSLAQVFALAEAYPDLKANTQF
                     LALQERISALDTAIAHRREFYNETVRINNVTIERFPNKLMAGWFKFRPAALLKFADG"
     assembly_gap    759..768
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(808..1380)
                     /locus_tag="PKN91_08395"
     CDS             complement(808..1380)
                     /locus_tag="PKN91_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNS28131.1"
                     /translation="MLGTLVLLVVVVVVAIGGIGIYNRLVALKHRVATSWSNIDVLLK
                     QRHDELPKLVDTCRQYMQYEQETLEKVMQARNAVYAARESGDVAGVSVAEGGLRAQLG
                     KLFAVAEAYPDLKANASFQQLQGRISELETGISDRRELYNEAVNVNNASIEQFPASII
                     AGMFAFKPAKLLEFADADKADVSVRALFSG"
     gene            complement(1396..2379)
                     /locus_tag="PKN91_08400"
     CDS             complement(1396..2379)
                     /locus_tag="PKN91_08400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNS28132.1"
                     /translation="MLPPLSVLDLAPVPQGTPASEAIRRTVDLARLADELGFARYWFA
                     EHHGMPSVASSSPEILIGHAAAATQRIRVGAGGIMLPNHAPLRIAEAFRTLAALHPDR
                     IDLGLGRAPGADRNASRALRAFDGEDFPHLVAEMLAFAAAERFEDGHPFRHVRAMPDD
                     VPLPPIWILGSSGASAAYAGKAGMGYSYASHFSPAPAAPAFRAYRDNFAPSVQFPRPH
                     TILGVAVVCAETTARARYLASTMQLAWLRIRRGQFLPLPDPDEASAYPYSTTELEAVR
                     EYEALTVVGNVQEVRARLAAMAADCGADELMVVTNVYDHGERLRSYELLAR"
     gene            complement(2390..2896)
                     /locus_tag="PKN91_08405"
     CDS             complement(2390..2896)
                     /locus_tag="PKN91_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HNS28133.1"
                     /translation="MEVRACTSQDLPAVQAIYAHHVLHGTGTFEEVPPSLEEMTARHA
                     GIVANDRFPFLVAVQDGAVLGFAYASAFGDRAAYRYTCESSVYIAPDAQRRGIGHALM
                     DQVIAQCTARGLREMLAVIGDSQNYASIGLHAALGFAHVGTFHNVGWKFERWIDVVLM
                     QRSLQPDR"
     gene            complement(2896..4284)
                     /locus_tag="PKN91_08410"
     CDS             complement(2896..4284)
                     /locus_tag="PKN91_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNS28134.1"
                     /translation="MSRYLSADILIQGSYLLTAFLFIMGLKRMSSPVTARSGILWAGA
                     GMALATLVTFLYPDMTNYPLIIAAIVVGALIAWWSGKRVAMTDMPQMIALYNGMGGGA
                     AAAIAAVELYRGGDHGLVTMVLAVAGGLIGAVAFSGSLIAFGKLQGLIKRSYRFGGQQ
                     FFNLLLLLVALVLGGMVALHLNASPAVISAFFAVALLLGLTMTLPIGGADMPVVISLY
                     NALTGLAVAFEGFVLGNAAMIIAGTVVGSAGTLLTQLMAKAMNRSLGNVLFSGFGESG
                     GGAAGAVTGSMKAIEAADVGVMMAFASKVIIVPGYGMAVAQAQHKVWELTQLLVDRGV
                     TVKFAIHPVAGRMPGHMNVLLAEAGVPYDLIADLDEINAEFETADVALVIGANDVVNP
                     VARNDRSSPIYGMPILNADKARNCIVVKRGQGAGFSGIENALFYLDNTRMLYGDGQKA
                     VAELIGAVKAIE"
     gene            complement(4286..4597)
                     /locus_tag="PKN91_08415"
     CDS             complement(4286..4597)
                     /locus_tag="PKN91_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNS28135.1"
                     /translation="MTGIVALYIFMLAAFTGYEVIAKVPVILHTPLMSGSNFVHGIVL
                     VGAMVALGSADTTLEKVIGFTGVLLAAGNAVGGYVVTERMLEMFKSSGAKRGDKSQGA
                     H"
     gene            complement(4629..5759)
                     /locus_tag="PKN91_08420"
     CDS             complement(4629..5759)
                     /locus_tag="PKN91_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNS28136.1"
                     /translation="MPVTIGVLREATPRESRVSLVPESATRFAALGARVLLERGAGIA
                     AQFPDALYKQVEWADTAAAVLNAADIVLTVQPLTTAQIGALRAGAVVVGHAQAHSKLA
                     EVAALRDGKITSFAMELVPRISRAQSMDALSSQAAIAGYKAVLIAADNLQKFMPMLTT
                     AAGTIRPSQVLVIGAGVAGLQAIATARRLGAVVEAYDVRSATREQVKSLGAKFVDVGV
                     SAEGAGGYARELTAEEQARQQEVLDARIAAADAVITTASVPGRPAPKIVSRGAVERMR
                     AGSVVVDIAAEQGGNCELTRAGETVVHQGVKVIGPVNLPAQLAYNASEMYARNLFNFL
                     SPALKGGELAIDWSDEVFAQACLTHDGVVKHEATAKALGTRG"
     gene            5880..6554
                     /locus_tag="PKN91_08425"
     CDS             5880..6554
                     /locus_tag="PKN91_08425"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNS28137.1"
                     /translation="MHGIDLDHADRGSVHDLHIIAGPQPGPLTQLVLRTDVSGMPLEW
                     IDYKEAARLYHQEQVAYTCGRSIFRLCGGTNARTGERTVLDVNSIVATVGHTGNPGNT
                     RHDYVPPLNNQTLFRRDAYLCMYCAQRFAARDLSRDHIRPFSQGGKDTWNNVVTACRR
                     CNNLKASRTPEQARMQLIAVPFTPTYAEYIFLKGRRVLTDQMEYLLAHFPRSSPLHAR
                     IQRWLA"
     gene            6568..7464
                     /locus_tag="PKN91_08430"
     CDS             6568..7464
                     /locus_tag="PKN91_08430"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNS28138.1"
                     /translation="MIYLVDASVYVFRAYYSMPPDMADGDGNPTHALFGFARFLGDLI
                     ERARPEFLAVAFDESLTSSFRNRLYPPYKANRDPAPTDLLWQFARCREFCEIAGVRHL
                     SSNEYEADDIIGTLAARARAGGLPFTVVTRDKDMAQLVREGDVYWDYAANERYRYHEI
                     EGRFGVAPERFADWLALTGDSVDNIPGVPGVGPKTATALMREFASLDDLYAGLERVAD
                     LPIRGASKLAEKLRRHRDAAYLARRLTGIACDMPLDLDHAALRPRAPDIAALNAFCDR
                     LGFGPMLRRQAERLAALAPGSG"
     gene            complement(7461..8219)
                     /locus_tag="PKN91_08435"
     CDS             complement(7461..8219)
                     /locus_tag="PKN91_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNS28139.1"
                     /translation="MLMVLVLAAGTSGAAQADCKFTATREGSADLAGVTKVVVAAGPG
                     ELSIVGSADARRIVARGNACASSQELLDRLNLEVRREGDTVFIDAGLARAINMITIGT
                     VYAHINVGIALPTNLPVEARDSSGPVSVSGVAALKLDDSSGDIEVRDVGSVEIKDSSG
                     DILVDGVRGDATVTFDGSGDIEIRDVGGNAEVGSDGSGNIRFSGVLGNVLVGNDGSGD
                     IVVENAGGDFTVGNDGSGTINHRGVAGKVTLPER"
     gene            8343..>10182
                     /gene="acs"
                     /locus_tag="PKN91_08440"
     CDS             8343..>10182
                     /gene="acs"
                     /locus_tag="PKN91_08440"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNS28140.1"
                     /translation="MTNKNIQSVLLEERLFDPPADFAARARIKPGDLAVLRSRAAADP
                     QGFWADLARTELVWDRPFTQVLDDSNAPHYRWFADGRLNVSANCLDRHLKARGHKTAL
                     IFEGEPGDTRRLSYRELHAEVCRLANALAKLGVTHGDRVVIYMPLIPESIIAMHACAR
                     IGAIHSVVFGGFSSVALRERIRDAGAKLVITADGGHRGGHVIPLKESTDRALAQDCES
                     VEHVIVCRRVGQPVTMRPGRDLWWHETVEGESPVCAPVPVDAEHPLFLLYTSGSTGKP
                     KGIQHSSAGFLLGAKVTAQWVFDLRDDDVFWCTADVGWVTGHSYNAYGPLAAGATIVM
                     YEGAPTIPDGGRFWKICETHGVTIFYTAPTAIRALMKLGEEIPARYDLSKLRLLGSVG
                     EPINPEAWMWYHRVIGRGRCPIVDTWWQTETGAIMISPIPGVTPTKPGSCTEPLPGIN
                     ADIVDEAGDPVTRPDAGGYLVIRDPWPFMLRTIWGDDARYRSTYWAKFQDRFYVAGDS
                     AHRDADGYYWIMGRIDDVLNVSGHRLGTMEIESALVAHTRVVEAAVVGRPHEIKGESV
                     FAFVVCSGARPGDDTAVLVQELREWVTEQLGALARPDDIRFADNLPK"
BASE COUNT         1615 a         3512 c         3398 g         1647 t
ORIGIN      
        1 gctcttctcg acgcgccagc ggctgttgtt cttgaagctg ccgcgcttct cggtgacgac
       61 atggaaccac aggcattcct gcatctggat cggatcgcgt accggtgcgc cgcccgccgc
      121 gcgggcggta ccggccagct cgacatagcc cttggcggcg gtcgcgacgc gcgaagtcgc
      181 cgtgccttcc atcaggctgg cggcgcgcat gtagcgtcgc cacatcacgt agccgctcgc
      241 ggcgatggcc acggcggcca cgaatgcctg cccgtcgtca gccatccgcg aacttcagca
      301 gcgcggccgg tcggaacttg aaccagccgg ccatcagctt gttcgggaaa cgctcgatcg
      361 tcacgttgtt gatgcgcacg gtctcattgt agaactcgcg ccgatgggcg atggctgtgt
      421 cgagcgccga aatgcgctcc tgcagcgcga ggaactgcgt gttcgccttg aggtcggggt
      481 aggcctcggc gagcgcgaac acctgggcga ggctgccgcg cagcgcctgc tccgcggcgc
      541 cgaccgattg cgcgttcgcg ccctgctggg cggcgaccgc cgcggcgcgc gcgcccgtca
      601 gccgcgcgag cagtgcggcc tcgaaccgcg agtactgccg gcagacatcg accagcttcg
      661 ggatctcgtc gttgcgctgc ttcagcagca cgtcaatgtt ggcccaggcc ttggtgacat
      721 tgtgcttcac cagcacgagc tggttgtaga cggcgacgnn nnnnnnnncg aacagcagca
      781 acaggacgac atagccggcc acggggctca gccgctgaac agcgccctga cgctgacgtc
      841 ggccttgtcg gcgtcggcga actccagcag cttcgccggt ttgaaggcga acatgccggc
      901 gatgatcgag gccgggaact gctcgatcga ggcgttgttg acgttgaccg cctcgttgta
      961 gagctcgcga cggtcggaaa taccggtctc gagctcgctg atgcgcccct gcagttgctg
     1021 gaacgaggcg ttcgccttca ggtccggata ggcctcggcg accgcgaaca gcttgccgag
     1081 ctgcgcgcgc aggccgccct cggcgacgct cacgccggcg acgtcaccgc tctcgcgtgc
     1141 cgcgtacacg gcattgcgtg cctgcatcac cttctcgagc gtttcctgct cgtactgcat
     1201 gtactgcctg caggtatcga cgagcttcgg cagctcgtcg tggcgctgct tcagcagtac
     1261 atcgatgttg gaccaggagg tcgcgacgcg atgcttgagc gcgaccaggc ggttgtagat
     1321 gccgatgccg ccgatggcga cgactacgac gacgaccagc agaacgagtg tgccgagcat
     1381 gtctgaccaa gcctcttatc gtgccagcaa ttcgtaggaa cgcagccgct cgccatgatc
     1441 gtacacattg gtgacgacca tcagttcgtc ggcaccgcag tctgccgcca tggcggcgag
     1501 ccgtgcgcgg acttcctgca catttccgac caccgtcagg gcttcatatt cgcgtaccgc
     1561 ctcgagttcg gtggtgctgt aggggtaggc gctcgcctcg tcgggatccg gcagcggcag
     1621 gaattggccg cggcggatcc tgagccacgc gagctgcatg gtcgaggcga ggtagcgggc
     1681 acgcgcggtg gtttccgcgc acaccacggc gacgccgagg atcgtgtgcg gccgcgggaa
     1741 ctgcaccgag ggcgcgaagt tgtcgcgata ggcgcgaaac gccggcgcgg cgggcgccgg
     1801 gctgaagtgg ctcgcgtagc tgtagcccat gcccgccttg ccggcgtagg cggcgctcgc
     1861 gccgctcgag ccgaggatcc agatcggcgg cagcggcaca tcgtcgggca tcgcccgcac
     1921 gtggcgaaac gggtgaccgt cctcgaagcg ctccgccgcg gcgaaggcga gcatctccgc
     1981 gaccagatgc gggaagtcct cgccgtcgaa ggcgcgcagc gcgcgcgagg cgttgcggtc
     2041 ggcgcccggt gcgcggccga gcccgaggtc gatccggtcg ggatggagcg cggcaagggt
     2101 gcgaaaggct tcggcaatgc gcagcggcgc gtggttcggc agcatgatgc cgcccgcacc
     2161 gacgcggatc cgctgcgtgg cggcggcggc atgcccgatc aggatctcgg gcgaggaact
     2221 cgcgacgctc ggcatgccgt gatgctcggc gaaccagtag cgcgcgaagc cgagttcgtc
     2281 ggcgaggcgc gcaaggtcga cagtgcggcg tatcgcctcc gatgccggcg tgccctgcgg
     2341 caccggtgcg aggtccagga ccgacagggg cggcagcatg gcttgcggtc tagcggtcgg
     2401 gctgcagcga gcgctgcatc aacacgacgt cgatccagcg ctcgaacttc cagcccacgt
     2461 tgtggaacgt gccgacgtgt gcgaagccga gcgcggcgtg caggccgatc gaggcgtagt
     2521 tctgcgagtc cccgatcacc gcgagcatct cgcgcaggcc ccttgcggtg cactgcgcga
     2581 tcacctggtc catcagcgcg tggccgatgc cgcggcgctg ggcgtcgggc gcgatgtaca
     2641 ccgaactctc gcaggtatag cggtaggcgg cgcggtcgcc aaaggcgctt gcgtaggcga
     2701 aacccagcac ggcgccgtcc tgtaccgcca cgaggaacgg gaaccggtcg ttcgccacga
     2761 tgccagcgtg gcgcgcggtc atttcttcca gggacggcgg cacctcctcg aaagtgccgg
     2821 tgccgtgcag cacatggtgc gcgtagatcg cctgcaccgc gggcaagtcc tgcgaggtac
     2881 aggcgcgcac ctccatcact cgatcgcctt caccgcgccg atcagttcgg cgacggcctt
     2941 ctggccgtca ccgtagagca tgcgcgtgtt gtcgagatag aagagggcgt tctcgatgcc
     3001 cgagaaaccc gcgccctggc cgcgcttcac cacgatgcag ttcctggcct tgtcggcatt
     3061 caggatcggc atgccgtaga tcgggctcga ccggtcgttg cgcgcgaccg ggttcaccac
     3121 gtcgttggcg ccgatcacga gcgcaacgtc cgcggtctcg aattcggcgt tgatctcatc
     3181 gaggtcggcg atcaggtcgt agggcacgcc cgcctcggcg agcagcacgt tcatgtggcc
     3241 cggcatgcgg cccgcgaccg gatggatcgc gaacttcacc gtgacaccac ggtcgaccag
     3301 cagctgcgtg agctcccaga ccttgtgctg ggcctgcgcc accgccatgc cgtaaccggg
     3361 cacgatgatg accttgctgg cgaaggccat catgacgccg acatccgccg cctcgatcgc
     3421 cttcatgctg ccggtgactg cgcccgccgc gccgccgcca ctctcgccga agccggagaa
     3481 cagcacgttg ccgagcgagc ggttcatcgc cttcgccatc agctgtgtca gcagcgtgcc
     3541 ggccgagccg accacggtgc cggcgatgat catggccgcg ttgccgagca cgaagccctc
     3601 gaaggcgacc gcgaggccgg tcagcgcgtt gtacagcgag atcacgaccg gcatgtcggc
     3661 gccgccgatc ggcagcgtca tcgtcaggcc gagcagcagc gcgaccgcga agaacgccga
     3721 gatgaccgcc ggcgaggcat tgaggtgcag cgccaccatg ccgccgagga ccagagcgac
     3781 gagcagcagc agcagattga agaactgctg gccgccgaag cggtatgagc gcttgatcag
     3841 gccctgcagc ttgccgaacg cgatcaggct gccgctgaat gccacggcgc cgatcagtcc
     3901 gccggccacc gccagcacca tcgtcacgag gccgtgatcg ccgccgcggt agagctccac
     3961 cgcggcgatc gccgccgccg caccgccgcc catgccgttg tagagggcga tcatctgcgg
     4021 catgtccgtc atcgcgacac gcttgccgct ccaccacgcg atcagtgcgc cgaccacgat
     4081 cgcggcgatg atcagcggat agttcgtcat gtccgggtag aggaacgtga cgagcgtggc
     4141 gagcgccatg cccgcgccgg cccacaagat gccgctgcgc gcggtcaccg gcgagctcat
     4201 gcgcttcagg cccatgatga acaggaacgc cgtgagcagg taactgccct ggatcaggat
     4261 gtcggcggag aggtagcggg acatctcagt gcgctccctg cgacttgtcg ccccgcttcg
     4321 cgccgctcga cttgaacatc tcgagcatgc gctcggtcac gacatagccg ccgaccgcgt
     4381 tgcccgctgc gagcagcacg ccagtgaagc cgatcacctt ctcgagcgtg gtgtcggccg
     4441 agcccagcgc gaccatcgcg ccgaccagca cgatgccgtg cacgaagttg gagcccgaca
     4501 tcagcggcgt gtgcaggatc accggcacct tggcgatcac ttcgtagccg gtgaaggcgg
     4561 caagcatgaa aatgtatagc gcgacgatac cggtcatgag tcactccaat ggacgttgtg
     4621 ccaggctctc agccccgggt ccccaaggcc ttcgcggtcg cctcgtgttt caccacgccg
     4681 tcgtgggtga ggcaggcctg ggcaaagact tcgtcggacc agtcgatcgc cagctcgccg
     4741 cccttgagcg cgggcgagag gaagttgaac aggttgcggg cgtacatctc gctcgcgttg
     4801 tacgcgagct gcgccggcag gttcaccggg ccgatcacct tcacgccctg gtgcaccacg
     4861 gtttcgccgg cgcgggtcag ttcgcagttg ccaccctgct cggcggcgat gtcgacgacc
     4921 accgatccgg cgcgcatgcg ctcgaccgcg ccgcgcgaga cgatcttcgg cgccgggcgc
     4981 cccggcacgg acgccgtggt gatcacggcg tcggccgccg cgatgcgtgc atcgagcact
     5041 tcctgctgcc ttgcctgctc ttcggcggtg agctcgcgcg cgtagccgcc ggcgccctcg
     5101 gcggacacgc cgacgtcgac gaacttcgcg ccgagcgact tcacctgttc gcgggtcgcg
     5161 ctgcgcacgt cgtaggcctc gaccacggcg ccgaggcgcc gtgcggtcgc gatcgcctgc
     5221 aggcccgcga cgccggcgcc gatcaccagc acctgcgagg ggcggatcgt gcccgccgcg
     5281 gtggtcagca tgggcatgaa cttctgcagg ttgtcggcgg cgatcagcac ggccttgtag
     5341 ccggcgatcg cggcctgcga ggacagcgca tccatcgact gggcgcgcga tatgcgcggc
     5401 acgagttcca tcgcgaagct cgtgatcttc ccgtcgcgca gcgccgcgac ttcggcgagc
     5461 ttcgagtggg cctgcgcatg gccgacgacg accgcgccgg cgcgcaacgc gccgatctgc
     5521 gcggtggtga gcggctgcac cgtcagcacg atgtccgcgg cgttgagcac ggcggcggcc
     5581 gtatccgccc attcgacctg cttgtagagc gcgtcgggga actgcgcggc gattcccgcg
     5641 ccgcgctcga gcagtacgcg cgcaccaagc gcggcgaacc tggtggcgga ctcgggaacg
     5701 aggctcacgc gcgattcccg gggcgtggct tctcgaagca cccctatcgt taccggcatc
     5761 tggcgggctc cctcatgcaa aaaagcgtgg cacaccgacc acgctccagg aaatgtctcg
     5821 tagaattagt acgttgcgac attgaattgt ggcacaaacc tgcataatct aacagcgcga
     5881 tgcacggtat tgatcttgat catgcggatc gcggttccgt ccacgatctg cacatcatcg
     5941 cggggccgca gccgggcccg ctcacccagc tcgtgcttcg caccgatgtc tcgggcatgc
     6001 ccctcgagtg gatcgactac aaggaagcgg ctcgcctcta tcaccaggag caggtcgcct
     6061 acacctgcgg ccgctcgatt ttccgcctct gtggcggcac caacgcgcgc accggcgagc
     6121 gcaccgtgct cgacgtcaat tcaattgtgg cgacggtcgg ccataccggc aacccgggca
     6181 acacccgcca cgactacgta ccaccgctca acaaccagac gctgttccgc cgcgacgcct
     6241 acctgtgcat gtactgcgcg cagcgcttcg ccgcccgcga tctgtcgcgc gaccacatcc
     6301 ggccgttcag ccagggcggc aaggacacct ggaacaacgt ggtcacggcc tgccgccgct
     6361 gcaacaacct gaaggcctcg cgcacgcccg agcaggcgcg catgcagctg attgcggtgc
     6421 cgttcacgcc gacctatgcc gagtacatct tcctcaaggg tcgccgcgtg ctgacggacc
     6481 agatggaata cctgctcgcg cacttcccgc gcagctcgcc gctgcatgcg cgcatccaga
     6541 ggtggctggc gtagtatccg ctgcgccatg atctacctcg tcgacgcttc cgtttacgtc
     6601 ttccgcgcct attactcgat gccgcccgac atggcggacg gcgacggcaa ccccacccac
     6661 gcgctgttcg gctttgcgcg cttcctcggc gacctgatcg agcgtgcccg gccggagttc
     6721 ctcgcggtgg cgttcgacga gagcctcacc tcgtcgtttc gcaaccggct ctacccgccc
     6781 tacaaggcga accgcgaccc ggcgcccacc gacctgctgt ggcagttcgc gcgctgccgg
     6841 gaattctgcg aaatcgccgg cgtgcgccac ctgtcgagca acgagtacga ggccgacgac
     6901 atcatcggta cgctcgccgc gcgcgcccgc gcgggcggcc tgccgttcac ggtggtcacg
     6961 cgcgacaagg acatggcgca actcgtgcgc gagggcgacg tgtattggga ctacgcggcg
     7021 aacgaacgct accgttacca cgagatcgag ggccgcttcg gcgtcgcgcc cgagcgcttc
     7081 gcagactggc tcgcgctcac cggcgacagc gtcgacaaca tccccggcgt accgggggtc
     7141 gggccgaaga ccgccactgc gctgatgcgc gagttcgcgt cgctcgacga tctgtatgcc
     7201 ggcctcgagc gggtggccga cctgccgatc cgcggggcgt cgaagctggc ggagaagctg
     7261 cgccggcatc gcgacgcggc gtatcttgcg cgccgactga ccggcatcgc ctgcgacatg
     7321 ccgctcgatc tcgaccacgc cgcgctgcgt ccgcgcgcac ccgatatcgc cgcactcaat
     7381 gccttctgcg accggctcgg cttcgggccg atgctgcgac gacaggccga gcggctcgca
     7441 gcgcttgcgc ccggatccgg ctagcgttcg gggagcgtga ctttgcccgc gacgcctcga
     7501 tgattgatgg tcccgctgcc gtcgttgccg accgtgaagt cgccgcccgc gttctcgacg
     7561 acgatgtcgc ccgagccgtc gttgcccacg agcacattgc ccaacacgcc ggagaagcgg
     7621 atattgcccg agccatcgct gccgacttcg gcattgccgc cgacgtcgcg gatctcgatg
     7681 tcgcccgacc cgtcgaaggt caccgtggcg tcaccgcgca cgccgtccac gagtatgtcg
     7741 cccgagctgt ccttgatctc gacgctgccg acgtcgcgta cctcgatgtc gcccgaggaa
     7801 tcgtcgagct tcagcgccgc gacaccgctc acgctcacgg ggccgctcga gtcgcgcgcc
     7861 tcgaccggca ggttcgtggg cagcgcgatg ccgacgttga tgtgggcgta aacggtgccg
     7921 atggtgatca tattgatggc ccgtgcgagg cccgcgtcga tgaagacggt gtcgccctcg
     7981 cgacgcactt cgagattgag cctgtccagc aattcctgac tggacgcgca ggcgttgccg
     8041 cgggcgacga tgcgtcgcgc gtccgcgctg ccgacgatac tgagttcccc gggtccggcc
     8101 gcgacgacga ccttcgtgac cccggccagg tcggctgatc cctcgcgcgt cgcggtgaac
     8161 ttgcagtccg cctgggccgc gccggaagtt cctgccgcca gaaccaggac catgagcatg
     8221 acgcctgtcc gcattgatgt ctccctgctg gcgccggaag gcgccggtgt tccgttggat
     8281 gcgcacaacc gccccggggt tgctacgcta ttaccctatg ggcgattgat cgagggctag
     8341 tcgtgaccaa caagaatatc cagtccgtcc tgctagagga acgacttttc gacccacccg
     8401 ccgactttgc cgcgcgcgcg cgcatcaagc ccggcgacct cgcagtactg cggtcgcggg
     8461 ccgcggccga tccgcagggt ttctgggccg atctcgcccg gaccgaactc gtctgggacc
     8521 ggccgttcac gcaggtgctg gacgattcca atgcgccgca ttaccgctgg ttcgccgacg
     8581 gacggctcaa cgtgtcggcc aactgcctcg accggcatct caaggcgcgc gggcacaaga
     8641 cggcgctgat cttcgagggc gagcccggcg atacccgccg gctgtcctat cgcgagctgc
     8701 acgccgaggt gtgccgtctc gccaacgcgc tcgcgaaact cggcgtcacc catggcgatc
     8761 gcgtggtgat ctacatgccg ctcatccccg agtcgatcat cgcgatgcac gcctgcgcgc
     8821 gcatcggcgc gatccactct gtcgtgttcg gcggcttctc ctcggtcgcg ttgcgcgagc
     8881 ggatcaggga cgccggcgcg aagctggtca tcacggccga tggcggccat cgtggcggcc
     8941 acgtcatccc gctgaaggag tcgaccgacc gggcgctcgc ccaggactgc gagtcggtcg
     9001 aacacgtgat cgtctgccga cgggtgggcc agcccgtcac gatgcgtccc gggcgggacc
     9061 tgtggtggca cgagaccgtc gagggcgagt ctccggtctg cgcgccggtc ccggtcgatg
     9121 ccgagcaccc gctgttcctg ctgtacacct cgggctcgac cggcaagccg aaaggcatcc
     9181 agcactcgag cgccggcttc ctgctcggcg ccaaggtcac ggcgcagtgg gtgttcgatc
     9241 ttcgcgatga cgacgtgttc tggtgcacgg ccgacgtggg ctgggtcacc gggcacagct
     9301 acaacgccta tggcccgctc gctgccggcg ccaccatcgt gatgtacgag ggcgcgccga
     9361 cgattcccga cggcgggcgc ttctggaaga tctgcgaaac gcacggcgtg acgatcttct
     9421 acaccgcgcc caccgcgatc cgcgcgctga tgaagctcgg cgaggagatt ccggcgcgct
     9481 acgacctctc gaagctgcgc ctgctcggtt cggtcggcga gccgatcaac ccggaagcat
     9541 ggatgtggta ccaccgcgtg atcgggcgcg ggcgctgccc catcgtcgac acctggtggc
     9601 agaccgagac cggcgcgatc atgatcagcc cgatcccggg cgtcacgccg accaaaccgg
     9661 gttcctgcac ggaaccgctg ccgggcatca acgccgacat cgtcgacgag gcgggcgacc
     9721 cggtcacgcg gcccgacgcg ggcggctatc tcgtcatccg cgatccatgg ccgttcatgc
     9781 tgcgaacgat ctggggcgac gacgcgcgtt atcgctcgac ctactgggcg aagttccagg
     9841 accgcttcta tgtcgcgggc gacagcgcac atcgcgacgc cgacggctac tactggatca
     9901 tgggccggat cgacgatgtg ctgaacgtct ccgggcatcg gctcggcacg atggagatcg
     9961 agtcggcgct ggtcgcgcat acgcgcgtcg tcgaggctgc cgtcgtcggc cgtccgcacg
    10021 agatcaaggg cgagtcggtg ttcgcattcg tggtgtgcag cggcgcgcgg ccgggcgacg
    10081 acacggcggt gctcgtccag gaactgcgcg agtgggtcac ggagcagctc ggcgcgctcg
    10141 cgcgcccgga cgacatccgc ttcgccgaca acctgccgaa ga
//
LOCUS       DAOQVY010000081        10160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_7408_length_10160_cov_6.417615, whole genome shotgun sequence.
ACCESSION   DAOQVY010000081 DAOQVY010000000
VERSION     DAOQVY010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10160
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7408_length_10160_cov_6.417615"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PKN91_08445"
     CDS             complement(<1..301)
                     /locus_tag="PKN91_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28141.1"
                     /translation="MTVFVTCIAVLAACSRERQDWKSAQAADTIESYGSFLAKHGDSA
                     LATQAEARIKQLGEERDWQDAATIDSLEAYQAYLRQHPESKWSQEARIRIENFALG"
     gene            377..772
                     /locus_tag="PKN91_08450"
     CDS             377..772
                     /locus_tag="PKN91_08450"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNS28142.1"
                     /translation="MSEQPRRRLLDQFGVSPTYIAEGSRIRGDIDTRGPFVLSGTLEG
                     NARVGAGLSISVGARLHGDVEARSAVIAGEVIGNVTIEEKLEIGATAVIHGSIAAKSL
                     AIANGAVIEGDIVVTGTQPVVKFDEKRRA"
     gene            complement(801..3632)
                     /locus_tag="PKN91_08455"
     CDS             complement(801..3632)
                     /locus_tag="PKN91_08455"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNS28143.1"
                     /translation="MGRTKLQGLTLAMLLAALGTLAACGGGGSDNPSLPPPPPPTDTT
                     PPSVPTNLAATASGPTQVQLTWTASTDASGVAGYRVYRNGSATPLASPTTNSYTDNSV
                     VASTTYSYTVRAFDAATPPNESALSAAAAVTTPATPPPPDTTPPSVPANVVATAPAPT
                     QVQLTWTASTDTGGSGLAGYRVYRNGSATPLASPTTNSYTDNSVVASTTYSYTVRAFD
                     AATPPNESALSAAANVTTPDAPGATPGLDTRPSNATCVAGDQPTSSLAVARVFPNLTF
                     SLPVQMLQAPGSAARWFVVEKGGVIRVFQNTPNVATSSVFLDITDRVLDDSTERGLLG
                     MAFHPQFPTDNRVFVFYIGTSPVETRISVFRTTDGGATLNPASEITLLTAARTQSNHN
                     GGNIMFGPDGLLYFGTGDGGGSNDQHGSIGNGQNLQTVLGKMLRIDVGGESATTYTIP
                     AGNPFAATGGSCTTGTTTAAQCREIYAYGFRNPWRWSFDRDTGELWVGDVGQNALEEI
                     DRVVSGGNYGWRCREGTQDTGMSCGTTGPFLPPVAEYGRTAGFSVTGGYVYRGTQIPS
                     LVGRYVFADYGTGRIWDIPSTTVPTLTVTSSQGFDTSLDISSFGEDVNGELYVVDLND
                     GELYQLVAGAAGGGTVATLLSASGCGASGDPAQPAPGLIPYAPNAGFWSDGIDKERWL
                     ALPDGATIDPDGGDWDLPNGSVLRKDFRHQGRLVETRLFMRHTNDNWAGYTYEWNAGG
                     TDATRVVGGKTVDLGSQDWIFPSEAQCLACHTQVAGRSLGLETAQLNGSILYPTTGRT
                     ANQIVTLDHIGMLNPGIPDDPATLPAMPDPYGSAGSVTERARAWLHTNCSFCHRPNGP
                     TPSSMDLRYTTALPSTNTCNVTPQSGDLGLPGTPRLIAPGAPESSVLLARIDTRAPDI
                     MMPPLASLLVDDEAVQLIQQWITGLANCN"
     gene            3766..4530
                     /locus_tag="PKN91_08460"
     CDS             3766..4530
                     /locus_tag="PKN91_08460"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNS28144.1"
                     /translation="MKPARIVHLVAHRGNAREYPENTLPALQSALDLGARFLEIDVHM
                     SADGVPVVIHDADLVRTTGVEGFVYDKPVAELVGIEAAERQRFGARYAGTCIPTLADA
                     AQLLDDRPEITLFVEIKRASLARFGHDQVVPQVLEAVRPWRTQVVIISFDLPAVFRAR
                     QIGGYRIGWVLPQYDSHTRLKCEALQPEFLFCDVDKLAAGEQLWRGTWRWVIYEVTEI
                     EQALSLAARGADFVESMAVRELGIAMRSRGRANLPA"
     gene            4533..5720
                     /locus_tag="PKN91_08465"
     CDS             4533..5720
                     /locus_tag="PKN91_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS28145.1"
                     /translation="MTRECDVAIVGGGIHGVGVAQAAAAAGHCVLLLEKSAIAAGTSS
                     RSSKLIHGGLRYLETGQMRLVRESLHERTALLRLAPGLVRLQPFYIPVYAETRRRPWV
                     LGTGLSLYAALAGFARGAGFGLLARREWDTLDGLDTRGLQHVFRYHDGQTDDAALARA
                     VLESARRLGAQALVPAAFLGADLHTGGVVVRYAAGGGVQECRARVLVNAAGPWAGSVA
                     RSVSPAVDIPDIELVQGTHLLLPGPPARGIYYVESPRDGRAIFVMPFRGQLMIGTTEV
                     RYRGDPDAVAATAGERHYLLGVLRRYFPAAAERALAGGGVPRAFAGLRVLPGGSGHAF
                     HRSRETQFALDREQAPRLISIYGGKLTTFRATAAHVMRLAAPSLPTRRATADVDRLPL
                     LPA"
     gene            5726..7168
                     /locus_tag="PKN91_08470"
     CDS             5726..7168
                     /locus_tag="PKN91_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNS28146.1"
                     /translation="MGTRETTWLALDQGGHASRALLFDAEGRELAEARVPIATRRIGE
                     DRVEHDPEELVTSLRTAASQALTAHSGRLPPVATGLATQRSSIVCWDRRSGAALSPVL
                     SWQDRRAAAALEPLRAEAHTVRRLSGLPLSAHYGASKMRWCVEHLPAVRDAARRGALR
                     IGPLATFLAGRLAGTSAKVDPANGSRTQLWDAARGDWSPELLDLFGIAREWLPDPGTT
                     VQDGLAQPGALALAVVTGDQSAVPFAWGEPREDTLYINLGTGAFLQRPTGVRRAEPGG
                     LLASVLFGDAQQTLYSLEGTVNGAASAIDWLAAREDLDVGAVWRRWESLDRAARAQPS
                     LLFLNGISGLGSPWWRSDFASRFVGEGDADARFAAVVASIAWLVRANVDELRRAGPIS
                     RAIVTGGLAQSDGLLALLADLCGIDLLRPAQGEATALGVFGLLRRDQDRAAADPADAA
                     GTVRVSADAGVARQAAAAFEHWHDAMRRAL"
     gene            complement(7169..8182)
                     /locus_tag="PKN91_08475"
     CDS             complement(7169..8182)
                     /locus_tag="PKN91_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNS28147.1"
                     /translation="MSGAADRYHPRAVIDTSIAPPRWIRGGHVQSILPSLPWRRPAIE
                     RRAQPMLAASVERLIDCGEGTVLQGFHARTGDGRGALAVLLHGWEGSARSLYVLSLAQ
                     TLFERGHELLRLNLRDHGETHHLNRELFHSCRLAELVGALRTIQAEAGGRPLQLVGFS
                     LGGNFMLRAAAAARDAGLAVAHTVAVSPVLDPKATLDALERGSPIYRRYFVLKWSRSL
                     ARKQAAWPGEYDFEALLRQGGLRHMTAELVRAHTHFPTLEEYLEGYAITGARLAGLDV
                     PTTIIAALDDPIIPPNDLCRLAAPDSLRIVLTPHGGHCGFFEQPNAPSWADRRVAQLL
                     ASV"
     gene            complement(8199..9635)
                     /locus_tag="PKN91_08480"
     CDS             complement(8199..9635)
                     /locus_tag="PKN91_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS28148.1"
                     /translation="MVTKRALIVDDSKSARAFLSRILTRYEIEVDTAESAEQAIEYLG
                     GQRPDVIFMDHLMPGMDGFQAVQVIKNNPRTATIPIMMYTSQEGELYVGQARALGAIG
                     VLPKQIQPTDVSKVLYQLKLIPDRRSDEQGGFEPVLPGQQAAGSASRPAAPPVAVNGA
                     GAAPATAAVGTSANVGAAHAAPVGDALPVGAAQLRSMVEEIVNEHAGAIRRSVNAHID
                     EQAVRLTQEVRAVVQEALPPAPAADEPRRRSLQWGWVAAAAALTAAAVLGAFWWREAE
                     SARALAGQLTVSREHVASLETQVAVFEAAEAARADEIASGVLASGTLADAGLLARALV
                     PYGEIPLSNDRLAVVRNALERLTAGNFHGVVEVTTYPGRFCLIGNATEGYSLAPDETP
                     VARCDLIGNPALEGTRPVQRESLAFANLVAESRRESGGAIDVRVATGEATDTALHYPE
                     VTASLTAGEWNRAAYANNRVELRARSAP"
     gene            complement(9686..>10160)
                     /locus_tag="PKN91_08485"
     CDS             complement(9686..>10160)
                     /locus_tag="PKN91_08485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS28149.1"
                     /translation="AQLLRVRPDALLVIAGEGPAREALTQLAQRLGIAGNARFIGYLD
                     RDAELLDCYAAGDLFVFASRTETQGLVLLEAMAQGTPVVSTAELGTRSILTAGSGGVV
                     LPESADAFVGAITALLADPAERQRLAALAREHASSWSSWTMARRLVALYRQLVLR"
BASE COUNT         1506 a         3505 c         3584 g         1565 t
ORIGIN      
        1 ccccgagtgc gaagttctcg atgcggatgc gcgcctcctg cgaccacttg ctctcgggat
       61 gctggcgcag ataagcctga tacgcctcga gcgagtcgat ggtcgcggcg tcctgccagt
      121 cgcgctcctc gccgagctgc ttgatgcgcg cctcggcctg ggtcgcgagc gcgctgtcgc
      181 cgtgcttcgc gaggaagctg ccgtacgact cgatcgtgtc ggcggcctgc gccgatttcc
      241 agtcctgacg ctcgcggctg caggccgcca ggaccgcgat gcaggtcacg aaaaccgtca
      301 tccgcaggat tgggtgcatg gctggtcgcg ctccctcgaa caagcttcgc tattctagcg
      361 cgaggaggag tgcacgatgt cagagcagcc caggcgccgc cttctcgatc agttcggggt
      421 gtcgcccacc tatatcgccg agggtagtcg gatccgcggc gacatcgata cgcgcggccc
      481 cttcgtgctg tcgggtacgc tggaaggcaa tgcgcgagtg ggcgccgggc tctcgatctc
      541 ggtcggcgcg cgcctgcacg gcgatgtgga ggcccggtcc gccgtcatcg cgggcgaagt
      601 catcggcaac gtgacgatcg aggagaagct agagatcggc gccaccgcag tcatccatgg
      661 cagcatcgcc gcgaaatccc tcgcgattgc caacggcgcc gtgatcgagg gcgacatcgt
      721 ggtcaccggc acgcagccgg tggtcaagtt cgacgaaaaa cgccgcgcct gatccgctcc
      781 gcccgcgcgc ctggcgcgcc tcaattgcag ttcgcgagtc ccgtgatcca ctgctggatc
      841 agctgcaccg cctcatcatc gacgagcagc gacgcgagcg gcggcatcat gatgtcgggc
      901 gcgcgcgtat cgatgcgcgc gagcaggacc gagctttccg gggcgccggg cgcaatgagg
      961 cgcggcgttc ccggcaggcc gagatctccg ctctgcggcg tcacattgca ggtattggtg
     1021 gacggcagcg ccgtcgtgta gcgcagatcc atgctgctcg gcgtcgggcc gttcgggcga
     1081 tggcagaacg agcagttcgt atgcagccac gcgcgcgcgc gctcggtgac cgagcccgcg
     1141 ctgccgtagg gatcgggcat cgcgggcaga gtcgcgggat cgtccgggat accgggattc
     1201 agcatgccga tgtgatcgag cgtcacgatc tggttcgccg tgcgcccggt ggtcgggtag
     1261 agaatgctgc cgttcagctg cgccgtctcg aggccgaggc tgcgcccggc gacctgcgta
     1321 tggcaggcga ggcactgcgc ttcgctcggg aatatccagt cctggctgcc gagatcgacg
     1381 gtcttgccgc ccaccacgcg cgtggcatcc gtgcctcccg cgttccactc gtaggtgtag
     1441 cctgcccagt tgtcgttcgt gtggcgcatg aacagacgcg tctcgacgag cctgccctgg
     1501 tgccggaaat ccttgcgcag cacgctgccg ttgggcagat cccagtcgcc gccgtccgga
     1561 tcgatcgtcg cgccgtccgg caatgcgagc cagcgctcct tgtcgatgcc atccgaccag
     1621 aaaccggcgt tcggcgcata cggaatgagt cccggggccg gctgcgccgg atcgccgctc
     1681 gcgccgcagc cacttgccga cagcagtgtc gccacggtac caccaccggc cgcgcccgcc
     1741 acgagctggt agagctcgcc gtcgttgagg tcgacgacgt agagctcgcc gttcacgtcc
     1801 tcgccgaacg aggagatgtc gaggctggta tcgaagccct ggctggaagt gactgtcagc
     1861 gtcggtacgg tcgtcgaggg gatgtcccag atgcgcccgg tgccgtagtc cgcgaacaca
     1921 taccggccga cgagcgaggg aatctgcgtg ccgcggtaca cgtagccccc ggtcacggaa
     1981 aagcccgccg tgcggccgta ctccgccacc ggcggcagga acggacccgt cgtgccgcaa
     2041 gacatgccgg tgtcctgcgt gccctcgcgg cagcgccagc catagttgcc gccggacacg
     2101 acccggtcga tttcctcgag ggcgttctgg ccgacgtcgc ccacccacag ctcgccggtg
     2161 tcgcgatcga agctccagcg ccagggattg cgaaagccgt aggcatagat ttcgcgacac
     2221 tgtgccgcgg tagtcgtgcc tgtggtgcac gaaccgccgg tggcggcaaa cgggttaccc
     2281 gccggaatcg tgtaggtcgt cgctgactcg ccgccgacat cgatgcgcag catcttgccg
     2341 agcaccgtct gcaggttctg gccattgccg atgctgccat gctggtcgtt cgagccgccg
     2401 ccgtcgcccg tgccgaaata cagcaggccg tcggggccga acatgatgtt gccgccgttg
     2461 tggttgctct gcgtgcgcgc ggcggtcagc agcgtgatct cgcttgccgg gttgagcgtc
     2521 gcaccgccgt cggtggtgcg gaacaccgag atgcgggttt ctaccggcga agtgccgata
     2581 tagaaaacga agacgcgatt gtcggtcggg aactgcgggt ggaaggccat gccgagcagg
     2641 ccgcgttcgg tcgaatcgtc gagcacgcga tcggtgatgt cgaggaagac gctgctcgtg
     2701 gcgacgttcg gcgtgttctg gaaaacacgg atcacgccgc ccttctcgac cacgaaccag
     2761 cgtgcggctg aacccggcgc ctgcagcatc tgcacgggca gcgagaacgt caggttcggg
     2821 aacacgcgcg ccacggcgag gctcgatgtc ggctgatcgc cggcaacgca ggtcgcgttc
     2881 gacgggcgcg tgtcgaggcc tggcgtggcg cccggcgcat ccggggtggt gacattcgcc
     2941 gccgccgaca gcgccgactc attcggcggc gtcgccgcgt cgaacgcgcg cacggtgtag
     3001 gaataggtgg tgctcgcgac caccgagttg tcggtgtagc tgttggtggt gggcgaagcg
     3061 agcggcgtcg cgctgccgtt gcggtacacg cgatagcccg caaggccgct gcccccggtg
     3121 tcggtcgagg cggtccaggt cagctgcacc tgagtcgggg caggcgccgt cgccacgaca
     3181 ttcgccggca cgctcggcgg ggtcgtatcg ggcggtggcg gcgtggcggg ggtggtgacg
     3241 gccgccgcgg ccgacagcgc cgactcattc ggcggcgtcg ccgcgtcgaa cgcgcgcacg
     3301 gtgtaggaat aggtggtgct cgcgaccacc gagttgtcgg tgtagctgtt ggtggtgggc
     3361 gaagcgagcg gcgtcgcgct gccgttgcgg tacacgcggt agccggcgac gccactggca
     3421 tcggtcgagg cggtccaggt cagctgcacc tgcgtcgggc ccgacgcagt ggccgcgagg
     3481 ttggtcggca cgctcggtgg cgtggtgtca gtaggcggcg gtggcggtgg cagtgacggg
     3541 ttgtccgagc ccccaccgcc gcacgcggcg agcgtgccca aagccgccag gagcatcgcg
     3601 agtgtcaaac cctgcagctt cgtgcgtccc atcggtcacc ccgcggatat actgatcgca
     3661 gtcaatccgg accatagctc ggaaacgaca catgtttgca caccggccac gcgcgcggaa
     3721 caggtgtggc tgtgcggaac tgccgacagc cgcccaccct gccccatgaa gccggcgcgc
     3781 atcgtccacc tggtcgcaca tcgcggcaat gcacgggaat accccgagaa caccctgccg
     3841 gcattgcagt cggcgctcga cctcggggcg cgattcctcg agatcgacgt gcacatgtct
     3901 gccgacggcg tgccggtggt gatccacgac gccgatctcg tccgcacgac cggcgtcgag
     3961 ggctttgttt atgacaaacc ggtcgccgag ctcgtgggca tcgaggccgc cgaacgccag
     4021 cgcttcggcg cgcgctacgc cggcacgtgc atccccacgc tggccgatgc cgcgcagctg
     4081 ctcgatgacc ggcctgaaat cacgctgttc gtggagatca agcgggcgag cctggcgcgc
     4141 ttcggccatg accaggtcgt gccgcaggtg ctcgaagcgg tgcgcccatg gcgcacccag
     4201 gtggtcatca tttccttcga cctgccggcc gtgttccgcg cccggcagat cggcggctat
     4261 cgcatcggct gggtgctgcc gcaatacgac agccacacgc gcctcaagtg cgaagcgctg
     4321 cagccggagt ttctcttctg cgacgtcgac aagctcgccg ccggcgaaca gctgtggcgc
     4381 ggcacctggc gctgggtgat ctacgaagtc accgagatcg agcaggcact gtcgctcgcg
     4441 gcgcgcggcg cggacttcgt cgaatccatg gcggtgcgcg aacttggcat cgcgatgcgc
     4501 tcgcgcgggc gcgcaaacct gcccgcgtga ccatgacccg cgagtgcgat gtcgcgatcg
     4561 tcggcggcgg catccacggg gtcggcgtcg cgcaggccgc cgcggccgcg ggccattgcg
     4621 ttctgctgct cgagaagtcg gcgatcgccg cgggcacctc gagccgctcg agcaaactga
     4681 tccacggcgg gctgcgctac ctcgagaccg gtcagatgcg tctcgtgcgc gaaagcctgc
     4741 atgagcgcac ggcgctgctg cgcctcgcgc ccggactcgt gcgcctgcag cccttctaca
     4801 ttcccgtata cgcggaaacg cgccggcgcc cctgggtgct cggtacgggc ctgtcactgt
     4861 acgcggcact cgctggtttc gcgcgcgggg cgggcttcgg gctgctcgcg cgccgcgaat
     4921 gggacacgct cgatggcctc gacacgcgcg ggctgcagca cgtcttccgc tatcacgacg
     4981 ggcagaccga cgatgcggcc ctcgcgcgtg cggtgctcga gtccgcacga cggctcggtg
     5041 cgcaggcgct cgtcccggcg gcattcctcg gcgcggacct ccatacgggt ggagtcgtcg
     5101 tgcgctacgc cgcgggcggc ggcgtgcagg agtgccgcgc gcgcgtgctc gtcaatgcgg
     5161 ccgggccatg ggcaggctcc gtggcgcgca gcgtctcgcc tgcagtcgac atcccggata
     5221 tcgagctcgt gcagggcacg cacttgctgc ttcccggccc gcccgcgcgc ggcatctact
     5281 acgtcgagag tccgcgcgac gggcgcgcga tcttcgtcat gccgtttcgt ggccagctga
     5341 tgatcggcac caccgaggtg cgctaccgcg gcgacccgga cgcggtggcc gccacggccg
     5401 gtgagcgcca ttacctgctc ggcgtgctgc gccgttattt tcccgcagcg gcggagcggg
     5461 cgctcgccgg cggcggcgta ccacgcgcct tcgccggcct gcgcgtactg ccgggcggat
     5521 cgggccacgc cttccaccgc tctcgcgaaa cgcagttcgc gctcgatcgc gagcaggcgc
     5581 cacgcctgat ttcgatctac ggcggcaagc tcacgacgtt tcgcgccacc gccgcgcacg
     5641 tcatgcgcct cgcggcgccg tccctgccga cgcggcgcgc gacagcggac gtcgaccggc
     5701 tgccgctgct acccgcctga gccgcatggg tacgcgcgaa acaacctggc tggcactgga
     5761 ccagggcgga catgcgagcc gggcgctgct gttcgatgcc gagggccgcg agctcgccga
     5821 agcgcgtgtg ccgatcgcaa cacggcgcat tggcgaagac cgcgtcgagc acgaccccga
     5881 agagctcgtc acctcgctgc gtacggcggc gtcgcaggcg ctcaccgcgc acagcggccg
     5941 cctgccgcct gtcgcaacgg gcctggccac gcagcgatcg agcatcgtgt gctgggaccg
     6001 gcgcagcggc gcggcactgt cgccggtgct ttcgtggcag gaccggcgtg ccgccgcggc
     6061 gctcgagcca ctgcgcgccg aggcgcatac ggtgcggcgg ctctcggggc tgccgctgtc
     6121 ggcgcactac ggcgcgagca agatgcgctg gtgcgtcgag cacctgcccg cagtacgcga
     6181 tgcggcccgg cgtggcgcgc tgcgcatcgg gccgctcgcc acgttcctcg ccgggcgcct
     6241 ggccggtacg agcgcgaagg tcgatcccgc aaacggctcg cgcacgcagc tgtgggatgc
     6301 agcgcgcggc gactggtcgc ccgaactgct cgacctgttc ggtatcgcac gcgaatggct
     6361 gccggatccc ggcacgaccg tgcaggacgg cttggcgcag cccggcgcac tcgcactggc
     6421 cgtcgtgacc ggcgaccagt cggccgtgcc attcgcctgg ggcgagccgc gcgaggacac
     6481 gctctatatc aatctcggca ccggagcctt cctgcaacga ccgaccggcg tccgccgtgc
     6541 cgagccgggc ggtctcctcg cgagcgtgct gttcggcgac gcgcagcaga cactgtattc
     6601 gctcgaaggc acggtcaacg gcgccgcgag cgcgatcgac tggctcgcgg cgcgcgaaga
     6661 cctggatgtc ggggccgtgt ggcggcgctg ggaaagtctc gaccgcgcgg cgcgcgcgca
     6721 gccctcgctg ctgttcctga acggcatctc cggcctcggt tcgccctggt ggcgcagcga
     6781 cttcgcctcc cgcttcgtcg gtgaaggcga tgccgatgcg cgctttgccg ccgtcgtcgc
     6841 gagcatcgcc tggctcgtgc gcgctaacgt cgatgaactg cgacgcgccg gccccatctc
     6901 gcgcgcgatc gtcacgggcg gcctcgcgca gtcggacggc ctgctcgcac tgctggccga
     6961 tctgtgtggc atcgacctgc tgcgtcccgc gcagggtgaa gccacggcgc tcggcgtgtt
     7021 cggcctgctg aggcgcgacc aggatcgcgc cgcggccgat ccggccgatg ccgcaggcac
     7081 ggtacgagtg agcgccgatg ccggagtggc acgtcaggcc gcagccgcct tcgagcactg
     7141 gcacgacgcc atgcgccggg cgctctgatc agacagaggc gaggagttgc gcgacgcgtc
     7201 ggtcggccca gctcggggcg ttcggctgct cgaagaagcc gcagtgccca ccgtgcggcg
     7261 tgagcacgat gcgcagcgag tcgggcgcgg ccagccggca caggtcattg ggcggaatga
     7321 tcggatcgtc gagtgcggcg atgatcgtcg tcggcacgtc gaggccggcg agccgcgcgc
     7381 cggtgatcgc atagccctcg aggtattcct cgagcgtcgg gaaatgggta tgtgcacgca
     7441 cgagctcggc ggtcatgtgc cgcagcccgc cctggcgcag cagcgcctcg aagtcgtact
     7501 cgccgggcca cgcggcctgc ttcctcgcga gtgagcggct ccatttgagg acgaagtaac
     7561 gccggtagat cggcgatcca cgctcgagcg cgtccagcgt ggccttcggg tcgagcaccg
     7621 gcgacacggc gacagtgtgg gcgacggcaa ggcccgcatc gcgcgcggcg gccgccgcac
     7681 gcagcatgaa attgccgccg agcgagaacc cgacgagttg aagcgggcgg cctcccgcct
     7741 cggcctggat cgtgcgcagc gcgccgacca gctccgcgag gcggcaggag tggaacagtt
     7801 cgcgattgag gtgatgcgtc tcgccgtggt cgcgcaggtt gaggcgcagc agctcatggc
     7861 cgcgctcgaa cagcgtctgc gcgagcgaca gcacgtacag cgaacgggcg ctgccctccc
     7921 agccgtgcag cagcacggcg agcgcaccgc gaccatcgcc cgtgcgggca tgaaagccct
     7981 gcagcaccgt gccttcgccg cagtcgatca ggcgctcgac gctcgccgcg agcatcggct
     8041 gcgcgcgccg ctcgatcgcc ggccggcgcc agggcaggct cggcaggatc gattgcacgt
     8101 ggccgccgcg gatccagcgc ggcggcgcga tcgaggtgtc gatgaccgcc cggggatggt
     8161 agcgatcggc ggctccgctc atggcggcct gcgcggcctc agggagcgga gcgcgcacgc
     8221 agctccacgc gattgttcgc gtaagccgcg cgattccact cgcctgccgt gaggctggcg
     8281 gtgacctcgg gataatgcag cgccgtgtcg gtggcctcac cggtcgcgac gcgcacgtcg
     8341 atcgccccgc cgctctcgcg ccggctctcg gcgacgaggt tcgcgaacgc aagcgattcg
     8401 cgctgcaccg gccgcgtacc ttcgagggcc ggattgccga tcaggtcgca gcgcgcgacc
     8461 ggcgtttcgt cgggagcgag cgagtaaccc tcggtggcat tgccaatgag gcagaagcgg
     8521 ccgggatagg tcgtcacttc gacgaccccg tggaaattcc cggccgtgag ccgctcgagc
     8581 gcattgcgca caaccgccag gcgatcgttc gacagcggta tttcaccgta cggcacgagc
     8641 gcgcgagcga gcagcccggc atcggcgagc gtgcccgagg cgagcacgcc ggaggcgatc
     8701 tcgtcggcgc gcgcggcctc ggcggcctcg aacacggcaa cctgggtttc gagtgaggcg
     8761 acgtgttcgc ggctcaccgt gagctgaccc gcaagcgcgc gcgcggattc ggcttcgcgc
     8821 caccagaagg caccgagcac cgccgccgca gtgagcgccg ccgccgcagc gacccagccc
     8881 cactgcaagc tgcgacgacg cggctcgtcc gcggccggcg cgggcggcag tgcctcctgc
     8941 acgaccgccc gcacctcctg ggtgaggcgg acagcctgct cgtcgatgtg cgcattgacc
     9001 gagcggcgta tcgcacccgc gtgctcgttg acgatctcct cgaccatgga acgcaactgc
     9061 gcggcgccta cgggcaatgc atcgcccacc ggcgccgcat gcgccgcccc gacgttcgcg
     9121 ctggttccga cggccgccgt cgcgggcgcc gcgcccgcgc cattcacggc aacgggcggt
     9181 gccgcaggac gtgacgcact gcctgcggcc tgctggccgg gcaacacagg ctcgaaaccg
     9241 ccctgctcgt cgctgcgccg atccgggatc agcttcaact ggtaaagcac tttcgacacg
     9301 tcggtcggct ggatctgttt cggcagcacg ccgatcgccc caagcgcgcg cgcctgcccg
     9361 acatacagct caccttcctg cgaggtgtac atcatgatcg ggatggttgc ggtacgcggg
     9421 ttgttcttga tgacctgcac ggcctggaag ccgtccatgc ccggcatcaa gtgatccatg
     9481 aagatcacgt cgggccgctg gccgccgagg tactcgatcg cctgctcggc ggattcggcc
     9541 gtatcgactt cgatttcgta gcgggtgagg atccgggaaa ggaacgcgcg cgccgacttg
     9601 gaatcatcga cgatcaacgc acgtttcgtc accatgcaga ctcccgcgca acgcgcgttg
     9661 gcagattggt acccggcgcc ggagtttagc gcagaacgag ctggcgatag agcgcgacaa
     9721 gtcgcctggc cattgtccac gacgaccagc tcgaagcatg ctcccgggcg agcgcggcga
     9781 ggcgctggcg ctccgcggga tccgcgagca gcgccgtgat cgccccgacg aatgcatccg
     9841 cggactcggg cagcacgacg ccgccgctgc ctgcggtcag gatggaacga gtgccgagtt
     9901 cggccgtcga caccaccggc gtgccctgcg ccatggcctc gagcagcacg aggccctgcg
     9961 tctcggtccg cgaggcaaac acgaacagat cgccggccgc atagcagtcg agcagttcgg
    10021 catcgcggtc gagatagccg atgaaccgcg cgttgcccgc gatgccgaga cgctgcgcga
    10081 gctgcgtgag tgcctcgcgc gccgggccct cgcctgcgat caccagcaac gcgtccggcc
    10141 ggacacgcag caactgcgca
//
LOCUS       DAOQVY010000082        10139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_7428_length_10139_cov_6.069119, whole genome shotgun sequence.
ACCESSION   DAOQVY010000082 DAOQVY010000000
VERSION     DAOQVY010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10139
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7428_length_10139_cov_6.069119"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="PKN91_08490"
     CDS             <1..891
                     /locus_tag="PKN91_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNS28150.1"
                     /translation="LFNALFSGPVRSLLDQSLGMIREREKGLRIKLHIDPEDASLARL
                     AGKHGIYLCGNAYETDSNFPGIYFQSCFVFDPDGSRILNYRRLTSMFSPTPHDYWDRY
                     LDLYGKESIFPVARTPIGNLAVIASEEIQYPELVRCFALRGAEVLCHPSSEPLMTGLT
                     PKDIAKRARAIENLAYVVSANTAGIEDVPYLEATCDGLSKIVDFEGRVLVEAGPGESA
                     AAVADLDIAALRRYRNRPGMNNFLSRMRMELYADTYSRKDFYPANTLNGIAPSREHFK
                     AAQHEVIDRLRSLGIIEPAD"
     gene            895..1635
                     /locus_tag="PKN91_08495"
     CDS             895..1635
                     /locus_tag="PKN91_08495"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNS28151.1"
                     /translation="MPTALITGAARGLGLGLLGRYLDAGWDVIAAVRDTGADTLAALH
                     ARHTSRLVVEHLDLADFESIGACARRVGGRPIDALIGNAAATHVSDRGFGQTDYDDWA
                     AVMRVNTFAQLRLAEAFVEHVARSQRRVMYFVSSRVGAKPIPGMMIAYRSSKSALNQV
                     VYQLALQLHARGICVACGHPGFVQTDATHGRGTFTAEESAGRLFSLIDRLQLADSGKF
                     FEPDGSELPIVTRQTNPRAVGSTPIVEA"
     gene            1659..2984
                     /locus_tag="PKN91_08500"
     CDS             1659..2984
                     /locus_tag="PKN91_08500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS28152.1"
                     /translation="MNQATAPYPSAARAWTIVTILTLAYIVSFLDRQILALLIEPIKV
                     ALELSDTQVGLLLGPAFAVFYVTLGYPAGLLADRKSRKVIIAAGITVWCIMTALCGLA
                     RDFRQLFAARVGVGVGEATLTPAALSMISDCFPRERRARPISVYMMGISLGGALAYLI
                     GGQLLEPLLERPPVEVPILGVIRPWQAAFLIVGLPGVLLAVLIAAIREPARQDVNRAL
                     GATTVRESMTTALTYIRKHWQAYSALTIGLSSNTIIGYVASWNVVLFQRRWGWGIADI
                     GLSLGLALLICGPLGTMIGGAISDRLSRAGHADSAMKVTMVAIVLMVVFGTLYPVVGA
                     PVTVLALFAAMVLAASAASATGAVALVTLSPNQIRAQATALYWIVKNLSGLLLGPVTV
                     GLLTDRVFSDPQQVGSSMAVASAFFGAIGIAALLLGARHYRASVSTAGQ"
     gene            complement(3010..3666)
                     /locus_tag="PKN91_08505"
     CDS             complement(3010..3666)
                     /locus_tag="PKN91_08505"
                     /inference="COORDINATES: protein motif:HMM:NF008102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNS28153.1"
                     /translation="MEFLYVLVDFVLHLDRHLVELLVRFDVWIYAILFLIIFAETGLV
                     VTPFLPGDSLLFAVGALAAVDTSGTLDPTIVAALLAFAAILGNTTNYAIGARIGPPAF
                     SGRYRLLKLEYLHRTEDFFRRYGPMTIVLSRFVPIVRTFAPFVAGVGRMPYGRFQAYN
                     IAGGTSWVVLFVVAGYLFGNLPWIKQNFGLVTILIIVVSLLPMAAMALRARRERATPR
                     "
     gene            complement(3670..4887)
                     /locus_tag="PKN91_08510"
     CDS             complement(3670..4887)
                     /locus_tag="PKN91_08510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNS28154.1"
                     /translation="MQVRSRTLLALATGAILGFSASMTGSVLADLRPGASRAVEALPW
                     EDARLLAEVLERVKRDYVDGVDDHALMENAVRGMVGALDPHSAFLDADEYDEIRASTT
                     GTYPGVGIEVAAEAEGIKVLRPIEGSPAFRAGIQSGDLIVAIDGEAIGVDLDHAIKRM
                     RGPAGSRITLTLARAGAPAPIDVALQRAQVAVHSVEHAMIEPGYGYLRITNFSETTAA
                     DVVRAIGALEHESKGVLSGLVLDLRNNPGGVLEAAVEVSDLFLESGIIVTAEGRTSDS
                     RFRMEASPGDELGGARMAVLVNGASASAAEILAGALKDHHRAVLVGRTTYGKGSVQTV
                     MPLSQGRAIKLTTSRYFTPSGASIQEKGITPDLLIDGKETLPDPAGVATDREVRVALD
                     ALKAGSRLARADH"
     gene            complement(4938..7184)
                     /locus_tag="PKN91_08515"
     CDS             complement(4938..7184)
                     /locus_tag="PKN91_08515"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HNS28155.1"
                     /translation="MAAPADNLRSNHWTTLEPYAQLVRSLVPRTSGIAVFDARANLRW
                     TSDSATWPELVSEVERSVREAADDADLPGQLRVLEGDVPLYLCWLRDDDTRELLAVVA
                     IQGRAGHESERRSFSFVHALIRPALECLRRELVSRHSLDLLAESLSARDRDLELVLAV
                     SRRDGARPGDDASALRVILENTVEHLGCALAALLVPEKSLVLFRMSQQNPADGRLLAQ
                     THRHLLSLGQVRRDPVIMNRLAQNSALGTLPYKILSCSLRNASTRPIGILALFRHEGA
                     ADFTDREARLVDVLARKASVIIEASYDAMTGLLNRQAFEQRADDLVRSGDKAGRFAVL
                     YIDVDQLHLVNENFGMHVGDLMLSMLADMMRRRLPPGAVAARISGDRFAVVMPGTLEA
                     ALELATSWCEFAAQISAVQGDARSRASLSIGVAEHSQPEGGLTHTLAAAETACKAAKD
                     RGRNRAEAYQAADESVVRRFTDITIAASVRAAVAEDRLRLDAQFILPLDATHGHPPHF
                     ELLLRMIDDEGVTIGPDRFLSAANRYQLMPTIDRWVIEHALEALRPHATVLAATRTVF
                     AINFSGQSLNDIEFADELISRIEASGIPPANLCFELTESATIANLARAEVLMRRLRKI
                     GCGVALDDFGTGLSSLAYLRQLPVSMLKIDGSFVRDIVHDARSESLVQAVAQLARSMQ
                     MTTVAEYVETGAIHERIAALGVDYGQGFSIGRPTPLAEILANLPSIVGEAPRLAIEVP
                     PEPAPGLH"
     gene            complement(7414..8589)
                     /locus_tag="PKN91_08520"
     CDS             complement(7414..8589)
                     /locus_tag="PKN91_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNS28156.1"
                     /translation="MSLLRSFPPIFLALLVAGLPAVIGSAGAQGRDAARAEAELKAVK
                     AEIAKIAAQVKRDSVNRNALTRNLRAAEVSVADARSELTRLRRERSERTTRRAALARE
                     KAGREAALGKEQRALGGELRSAYLIGREEPLRLLLNQEDPARAGRMFAYYSYFGRARA
                     DRIASIEAHVAEIAALDAQLQAEEERLVALEAQQKEQVAQLEAARAERGKALAALDAA
                     SRSRAAKLEELRSQQAGLEKLVRDLRRALEQFPVDPTAAFAKLRGKLAWPTTGRIVAT
                     YGTTRAGAIKWDGMMIATARGAPVRSVASGRIAYADWLPGLGLLVIVDHGGGYLSLYG
                     HNDQLYKAVGERVAPGDTIAGAGDSGGRSRPELYFEIRRAGRPIDPRPWFQRAAPRP"
     gene            8653..9081
                     /locus_tag="PKN91_08525"
     CDS             8653..9081
                     /locus_tag="PKN91_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNS28157.1"
                     /translation="MDRLLEYAANHPWLVGATGLALVMVIAYEVRARATSFASVAPQE
                     AIRLMNQHALLLDIRRPEQFAEGHIGGAKHMPSDQILTAGETMKKHREKPIVVYCDSG
                     SLAAAAVRQLTAQGFTKAVNLRGGLAAWRAEGLPVIKGTK"
     gene            9078..9353
                     /gene="grxC"
                     /locus_tag="PKN91_08530"
     CDS             9078..9353
                     /gene="grxC"
                     /locus_tag="PKN91_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HNS28158.1"
                     /translation="MTAPKVVMYATSWCPYCARARNLLASKGVAIEEIDVEAAPGNRQ
                     EMQARSGRRTVPQIFIGDTHVGGSDDLHALDAAGKLDSLLGRTSSEH"
     gene            9343..9813
                     /gene="secB"
                     /locus_tag="PKN91_08535"
     CDS             9343..9813
                     /gene="secB"
                     /locus_tag="PKN91_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HNS28159.1"
                     /translation="MSTEAAGAVPADTTAPQLALQLVYLKDCSFEAPQGPRVDGAWNP
                     QINLDLNTTVSPLGDDLREVLLTVTVSAKHGEQTTFLVEVKQAGAFLMRNLSETEAKR
                     ALTSVCPGVLFPYARAAVSNLVTLGGFPQFLLPPVNFEALYARSVADSQSATKN"
     gene            9815..>10139
                     /locus_tag="PKN91_08540"
     CDS             9815..>10139
                     /locus_tag="PKN91_08540"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNS28160.1"
                     /translation="MFAGATLQAQIAVLGAGSWGTALAIQCARKGNAVRLWSRNGESL
                     ARIGAARRNERYLPDAAFPASLSVEADLARAVRQARDVIIAVPSHAFRTVLTDLAEVA
                     EAETGI"
BASE COUNT         1628 a         3427 c         3452 g         1632 t
ORIGIN      
        1 ctcttcaacg ccctctttag cggcccggtc cgctcgctcc tcgaccagag cctgggtatg
       61 atccgcgagc gggaaaaagg gctgcgcatc aagctccaca tcgaccccga ggacgccagc
      121 ctggcccgcc tggcgggcaa gcacggcatt tacctctgcg gcaatgccta cgagacggac
      181 tcgaactttc cgggcatcta tttccagtcc tgcttcgtct tcgaccccga cggctcccgt
      241 atcctcaatt accggcggct cacgtcgatg ttctcgccga cgccgcacga ctactgggat
      301 cgttacctcg atctctacgg caaggaatcg atatttccgg tggcgcgtac gccgatcggc
      361 aacctagcgg tgatcgcatc cgaggaaatc cagtacccgg agctggtccg ctgtttcgcg
      421 ctgcgcggcg ccgaagtcct gtgtcacccg agctccgagc ctctgatgac cggactcacg
      481 ccgaaggaca tcgccaagcg ggcgcgcgcg atcgagaatc tcgcgtatgt cgtgtcggcg
      541 aacactgccg ggatcgagga cgtgccgtat ctcgaggcca cctgcgacgg cctttccaag
      601 atcgtggatt tcgagggccg cgtgctcgtg gaagccgggc caggcgaatc cgccgccgcc
      661 gttgcggatc tcgacatcgc ggcgctgcgc cgctaccgca accggccggg catgaacaac
      721 ttcctctcgc gtatgcggat ggagctgtac gcggatacct acagtcgcaa ggacttctat
      781 cccgccaaca ccctcaatgg cattgcccct tcacgcgaac acttcaaggc ggcgcagcat
      841 gaagtgatcg accggctgcg cagtctcggc atcatcgagc cggcggactg agcgatgcct
      901 accgccctga ttactggcgc cgcgcgcggg cttggactgg gactgctcgg ccgttatctc
      961 gacgccggct gggacgtgat cgccgcggtg cgcgacaccg gggccgacac gctggcggca
     1021 ctgcatgccc gacacacgtc ccggcttgtg gtagaacatc tcgacctcgc ggacttcgag
     1081 tcgatcggtg cctgcgcccg gcgcgtcggc gggcggccga tcgacgcgct gattggcaat
     1141 gccgcggcaa cccatgtctc cgatcgcggc ttcggacaga ccgactacga cgactgggca
     1201 gcggtgatgc gcgtaaacac atttgcccaa ctgcggctcg cggaagcgtt cgtcgagcac
     1261 gtggctcgca gccagcgccg cgtcatgtat ttcgtgtcga gcagggtcgg cgcaaaaccg
     1321 atccccggaa tgatgatcgc ctatcgcagc tccaagtcgg cgctgaacca ggtcgtgtac
     1381 cagctcgcgc tgcagttgca cgcgcgcggc atctgcgtgg cctgcggaca cccgggcttc
     1441 gtgcagaccg atgccacgca cgggcgcggc acattcaccg ccgaggagag cgccgggcgg
     1501 ctgttttccc ttatcgaccg gctgcagctc gcagattcgg gcaaattttt cgaaccggac
     1561 ggctcggagc tccccatcgt cacgcgccag accaatccgc gcgccgtggg cagcactccg
     1621 attgtcgagg cgtaacccac gatgcagcag tcctccgcat gaaccaggcc acagcgcctt
     1681 atccgtccgc ggcccgcgcc tggaccatcg tcacgatcct gacgctcgcg tacatcgtct
     1741 ccttcctcga ccggcagata cttgcgctgc tgatcgagcc gatcaaggtg gcgcttgagc
     1801 tttccgacac gcaggtgggc ctgctgctcg gcccggcttt cgccgtattc tacgtaacgt
     1861 tgggttaccc cgcgggattg ctggccgacc gcaagagccg caaggtcatc atcgccgccg
     1921 gcatcacggt ctggtgcatc atgaccgcgc tgtgcgggct ggctcgggat ttcaggcagc
     1981 tgttcgcggc gcgcgtcgga gtcggtgtcg gagaggcgac ccttacgcca gccgcgctgt
     2041 cgatgatcag cgactgcttc ccgcgcgagc ggcgcgcgcg gccgatcagt gtctacatga
     2101 tgggcatctc gctgggcggc gccctcgcgt acctgatcgg cggccagctg ctcgaaccgc
     2161 tgctcgagag accaccggtc gaggtgccga tactcggggt catccggcct tggcaggctg
     2221 cattcctgat cgtcggcctg ccgggcgtgc tgctggcggt gctgatcgcg gcaatccgcg
     2281 aacccgcacg gcaagacgtg aatcgggctc tcggcgccac cacggtgcgc gagtccatga
     2341 caacggccct gacctatatc cgcaagcact ggcaggccta ctccgcgctg acgatcggac
     2401 tcagttcaaa caccatcatc ggctacgtgg catcgtggaa tgtcgtgcta ttccagcggc
     2461 gctggggctg gggtattgcc gatatcggcc tctcactggg cctcgcattg ctgatctgcg
     2521 gaccgctggg cacgatgatc ggcggggcga tatcggatcg actctcgcgg gccggtcacg
     2581 ccgactcggc gatgaaggtc accatggtcg ccattgtact gatggtcgtc ttcggcacgc
     2641 tttacccggt cgtcggcgcg ccggtcacgg tgctcgcact gtttgcggcc atggttctgg
     2701 cggcatcggc ggcgtcggcg accggggccg ttgcgctcgt cacactgagt ccgaatcaga
     2761 tccgggccca ggccacggca ctctactgga tcgtcaagaa tctctctggc ctgctacttg
     2821 ggccggtaac ggtcggcctg ctgaccgacc gggtcttcag cgatccacag caggtcggca
     2881 gcagcatggc ggttgcatcc gccttcttcg gggcgatcgg catcgccgcg ctgctgctgg
     2941 gtgcccggca ctatcgagcc agtgtgtcga ccgcggggca gtaaagcctg ccgctcaccg
     3001 gcggcgcact catcggggcg ttgcgcgctc tcgtctagcg cgcagggcca ttgcggccat
     3061 cggcagcagc gacacgacga tgatcaggat cgtcaccaac ccgaagttct gcttgatcca
     3121 cggcaggttg ccgaacaggt aacccgcgac gacgaacagc acgacccagc tcgtgccgcc
     3181 ggcgatgttg taggcctgga agcgcccgta gggcatgcgc ccgacgccgg cgacgaacgg
     3241 cgcgaaggtg cgcacgatcg gcacgaagcg cgacagcacg atcgtcatcg gcccgtagcg
     3301 gcggaagaaa tcctcggtgc ggtgcaggta ttcgagcttc agcaggcgat agcggccgct
     3361 gaacgccggc ggcccgatgc gcgcgccgat cgcgtagttg gtcgtgttgc cgagtattgc
     3421 ggcgaacgcg agcagcgcgg cgacgatggt cggatcgagc gtgccgctgg tgtcgaccgc
     3481 ggcgagcgcg cccaccgcaa acagcagcga gtcgccaggc aggaagggcg tcacgacgag
     3541 gccggtctcg gcgaagatga tcaggaagag gatcgcgtag atccacacat cgaagcgcac
     3601 cagcagctcg accaggtgac ggtcgaggtg cagcacgaaa tcgacgagga cgtacaggaa
     3661 ttccaaacgt cagtggtcgg cgcgggccag gcggctgccg gccttcagcg catcgagcgc
     3721 aacgcgcact tcgcggtcgg ttgcgacgcc ggccgggtcg ggcagcgttt ccttgccgtc
     3781 gatcagcagg tcgggcgtga tgcctttttc ctggatcgac gcgcccgagg gcgtgaagta
     3841 acgcgaggtc gtgagcttga tggcacggcc ttgcgacaac ggcatgacgg tctgcacgga
     3901 gcccttgccg taggtggtgc ggccgacgag caccgcgcga tggtgatcct tgagcgcgcc
     3961 ggcgaggatc tcggccgccg atgccgacgc gccattcacc agcaccgcca tgcgcgcgcc
     4021 cccgagctcg tcgccgggcg aggcctccat gcggaagcgc gaatctgagg tgcggccctc
     4081 ggccgtcacg atgatgccgg attcgaggaa caggtcgctc acctcgaccg cggcttcgag
     4141 cacgccgccc ggattgttgc gcaggtcgag caccaggccc gacagcacgc ccttcgactc
     4201 gtgctcgagc gcgccgatag cacgcacgac gtcggcggcg gtggtctcgc tgaaattggt
     4261 gatgcgcagg taaccgtagc cgggctcgat catcgcgtgt tcgacactgt ggaccgccac
     4321 ctgcgcacgc tgcagcgcca cgtcgatggg cgcgggcgcg ccggcgcgcg cgagcgtcag
     4381 ggtgatgcgg ctgcccgcgg gtcctcgcat gcgcttgatc gcatggtcga ggtcgacgcc
     4441 gatcgcctcg ccgtcgatcg cgacgatcag gtcgcctgac tggatgccgg cgcgaaacgc
     4501 cggcgagccc tcgatcggcc gcagcacctt gatgccttcg gcttcggcgg ccacctcgat
     4561 gccgacgccc ggataggtgc cggtggtgct cgcgcggatc tcgtcgtatt cgtcggcatc
     4621 gaggaaggcc gagtgcgggt cgagcgcgcc caccatgccg cgcaccgcat tttccatcag
     4681 cgcgtggtcg tcgacgccgt cgacatagtc gcgcttcacc cgttccagca cttcggcgag
     4741 caggcgcgcg tcttcccagg gcagcgcttc gaccgcgcgc gacgcaccgg ggcgcaggtc
     4801 ggcgagcacg ctgccggtca tcgacgcgga gaacccgaga atggccccgg tggccagcgc
     4861 gagaagggtg cgggatcgga cctgcatgcg gtgattacta cacagcccgg ggccggcggc
     4921 aagccgcggg gataccccta gtggagcccc ggggccggct cgggcggcac ctcgatggcc
     4981 agccgcgggg cctccccgac gatcgagggc agattggcga gaatttcggc caagggggtc
     5041 gggcggccga tcgagaagcc ctgcccgtag tcgaccccca gtgctgcgat gcgctcgtgg
     5101 atcgcgccgg tttcgacata ttcggccacg gtcgtcatct gcatcgagcg ggcgagctgc
     5161 gccacggcct gcaccagcga ttccgagcgc gcatcgtgca cgatgtcgcg cacgaagctg
     5221 ccgtcgatct tgagcatcga tacgggcagc tggcgcaggt acgcgagcga ggacaggccg
     5281 gtgccgaaat cgtcgagcgc gacgccacag ccgatcttgc gcaggcgccg catcagcact
     5341 tcggcgcgcg ccaggttcgc gatggtcgcg ctctcggtga gctcgaagca gagattcgcc
     5401 ggcgggatgc cgctcgcctc gatgcgcgag atgagctcgt cggcgaactc gatgtcgttg
     5461 agtgactgac cggagaagtt gatcgcgaac accgtgcggg tcgccgccag cacggtggca
     5521 tgcggccgca gtgcctcgag cgcatgctcg atcacccagc gatcgatggt cggcatcagc
     5581 tggtagcgat tggccgcgga caggaagcga tcggggccga tggtcacgcc ctcatcgtcg
     5641 atcatgcgca ggagcagttc gaaatgcggc ggatgtccgt gcgtcgcatc gagcggcagg
     5701 atgaactgtg catcgagtcg cagcctgtcc tcggcgacgg ccgcgcgcac cgaggcggcg
     5761 atcgtgatgt cggtgaagcg ccgcaccacg ctttcatccg cggcctggta ggcctcggcg
     5821 cgattgcggc cgcgatcctt ggcggccttg caggcggttt cggcggccgc gagcgtgtgg
     5881 gtgagcccgc cctccggctg cgagtgctcc gcgacaccga tgctgagcga ggcgcgcgag
     5941 cgcgcgtcgc cctgcacggc ggaaatctgc gcggcgaact cgcaccacga cgtcgcgagt
     6001 tcgagcgccg cctctagcgt gcccggcatc accaccgcga agcggtcgcc ggagatgcgt
     6061 gccgccacgg cgcccggcgg caagcgccgg cgcatcatgt cggcgagcat ggagagcatc
     6121 aggtcgccga cgtgcatgcc gaagttctcg ttgacgaggt gcagctggtc gacgtcgatg
     6181 tagagcacgg cgaatcgccc ggctttatca ccggacctga ccaggtcgtc cgcgcgctgc
     6241 tcgaatgcct ggcggttcag caggccggtc atcgcgtcgt aactcgcctc gatgatcacc
     6301 gaggccttgc gggccagcac gtcgacgagc cgcgcctcgc ggtcggtgaa gtcggcggcg
     6361 ccctcgtggc gaaaaagcgc aaggatgccg atcgggcgtg tcgaggcatt gcgcagcgag
     6421 caggaaagga tcttgtacgg cagcgtgccc agcgcggagt tctgggcgag ccggttcatg
     6481 atgaccggat cgcgacgcac ctgcccgagc gacagcaggt gccggtgagt ctgggcgagc
     6541 agccggccgt cggcgggatt ctgctgcgac atgcgaaaga gcacgagact cttctccggc
     6601 acgagcagcg cggccagcgc gcagccgagg tgctcgacgg tgttctcgag gatcacgcgc
     6661 agcgccgagg cgtcgtctcc ggggcgcgcg ccgtcgcggc gtgacaccgc cagcacgagc
     6721 tcgaggtcgc gatcgcgcgc gctcaggctc tcggccagca ggtcgaggct gtggcgcgag
     6781 accagttcgc ggcgcagaca ttcaagcgcg ggccggatca atgcgtgcac gaacgagaac
     6841 gagcgccgtt cgctttcgtg gcccgcgcga ccctggatcg ccaccacggc aagcagttcg
     6901 cgcgtgtcgt cgtcgcgcag ccagcacagg tagagcggca catcgccctc gagcacgcgc
     6961 agctggccgg gcaagtcggc atcgtcggcg gcttcccgca ccgagcgctc gacttcgctc
     7021 accagctcgg gccaggtggc cgaatcgctg gtccagcgca gattggcgcg cgcatcgaac
     7081 acggcgatgc ccgaggtgcg aggaacgagc gaacgcacga gctgcgcata cggctcgagg
     7141 gtggtccagt gattcgagcg gagattgtct gccggcgcag ccatcgatcc ttccctatgg
     7201 cgtgggccgc accatgtcac acccgggcat ttcacggtgt tgatcgagtt ggcgtttcgt
     7261 caaatcgttg cacccgggcg agtctgcggt gccacgctcg cccggcgcag cgccaaatcg
     7321 ggcgcgaatg gccgctaatg tgtgatgcgc atcacacgca ccggcgccgc ggagcggggc
     7381 ggggcggggc ggggcgcagc gccggagcac gcgtcacggt cgcggggcgg cgcgctggaa
     7441 ccagggccgt ggatcgatcg ggcgccccgc gcggcggatc tcgaagtaca gctccggccg
     7501 gctgcgcccg ccgctgtcgc cggcgccggc gatggtgtcg ccgggtgcga cccgctcacc
     7561 caccgccttg tacagctggt cgttatggcc gtagaggctc aggtaaccgc cgccgtgatc
     7621 gacgatcacg agcagaccga gtcccggcaa ccagtccgca tacgctatgc gtccgctcgc
     7681 cacactgcgc accggcgcgc cgcgcgcggt cgcgatcatc atgccgtccc acttgatcgc
     7741 accggcacgt gtggtgccat aggtggccac gatcctgccg gtcgtgggcc acgcaagttt
     7801 gccacgcagc ttggcgaacg ccgccgtggg gtcgacgggg aactgctcga gcgcgcgtcg
     7861 caggtcgcgc accagctttt cgagcccggc ctgctgcgaa cgcaactcct cgagcttcgc
     7921 cgcacgcgag cgcgatgcgg catcgagtgc ggcgagtgcc ttgccgcgct cggcgcgcgc
     7981 ggcctcgagc tgcgcgacct gctccttctg ttgcgcctcg agcgcaacga gacgctcctc
     8041 ctccgcctgc aactgcgcat cgagcgccgc gatttcggca acgtgcgcct cgatgctcgc
     8101 gatgcgatcg gcccgggcgc ggccaaaata gctgtagtag gcgaacatgc ggccggcgcg
     8161 cgcggggtcc tcctgattca gcagcaggcg cagtggctcc tcgcggccga tcaggtaggc
     8221 gctgcgcagt tctccgccga gcgcccgctg ctccttgccg agcgccgcct cgcgcccggc
     8281 cttctcgcgc gcgagcgcgg cgcgtcgggt ggtgcgctcg ctgcgctcgc gccgcaggcg
     8341 cgtgagctcg ctgcgcgcat ccgccaccga tacctcggcg gcccgcaggt tgcgagtcag
     8401 cgcgttgcgg ttgacggagt cgcgcttgac ctgggcggcg atcttcgcga tctcggcctt
     8461 gactgccttg agctcggcct cggcacgggc cgcgtccctg ccctgggccc cggcgctccc
     8521 gatgaccgcg ggtaggcccg ccaccagcaa tgcgaggaaa atcggaggaa aagaccgcag
     8581 caacgacatc gaggctagtc taacgcagcg cccccgctat aattcgcggc ccttttccga
     8641 ggttcgcgct ccatggaccg attgctcgag tacgccgcca accacccctg gctagtgggt
     8701 gcgaccggcc tcgcgctcgt gatggtgatc gcctacgagg tgcgcgcgcg cgcgaccagc
     8761 ttcgcatcgg tagcgccgca ggaggcgatc cggctgatga atcagcacgc cctgctgctc
     8821 gatatccgca ggcccgagca gttcgccgag gggcacatcg gtggtgcgaa gcacatgcct
     8881 tcggaccaga tcctgacggc gggagagacg atgaagaagc atcgcgagaa accgatcgtc
     8941 gtgtactgcg atagcggctc gctcgccgcg gcggcggtcc gccagctgac ggctcagggt
     9001 ttcaccaagg cggtcaacct gcgcggtgga ctcgccgcgt ggcgcgccga aggcctgccg
     9061 gtcatcaagg gcacgaaatg acggcaccca aagtcgtcat gtatgccacg agctggtgcc
     9121 cctactgtgc acgtgcccgc aacctgcttg cctcgaaggg tgtcgcgatc gaggagatcg
     9181 acgtcgaggc cgcgcccggc aaccggcagg agatgcaggc gcgcagcgga cggcgtaccg
     9241 tgccgcagat tttcataggc gacacgcacg taggtggatc cgacgatctg catgcgctcg
     9301 acgccgcagg caaactcgat tcgctactcg ggagaacatc aagtgagcac tgaagccgcc
     9361 ggcgccgtac ccgccgatac gaccgcaccg caactcgcgc tgcagctcgt gtacctcaag
     9421 gactgttcgt tcgaagctcc gcagggaccg cgggtcgacg gcgcctggaa cccgcagatc
     9481 aacctcgacc tgaacaccac ggtctccccg ctcggcgacg acctgcgcga agtgctgctg
     9541 accgtcaccg tgtcggcgaa gcatggcgag cagacgacat tcctcgttga agtgaagcag
     9601 gccggcgcgt tcctgatgcg caacctctcg gaaacggagg cgaagcgcgc cctgacgagc
     9661 gtgtgccccg gcgtcctgtt tccgtatgcg cgcgccgcgg tatcgaacct cgtgacgctc
     9721 ggcggcttcc cgcagttcct gcttccgcca gtgaacttcg aggcgctgta cgcccgctcg
     9781 gtggccgaca gccaaagcgc caccaagaac tgacgtgttc gcgggcgcca ccttgcaggc
     9841 gcagattgcc gtgctcggcg ccgggtcgtg gggcacggcg ctcgctatcc agtgcgcccg
     9901 caagggcaat gcggtgcgcc tgtggagccg caacggcgaa tcgctcgcgc gcatcggtgc
     9961 cgcgcgccgc aacgagcgct acctgcccga cgccgcgttt cccgcctcgc tctcggtcga
    10021 ggccgatctc gcccgcgcgg tgcgccaggc gcgcgacgtg atcatcgcgg tgccgagcca
    10081 cgcatttcgc acggtgctga ccgacctcgc ggaagtcgcg gaggccgaga ccggcatta
//
LOCUS       DAOQVY010000083        10125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_7443_length_10125_cov_5.325621, whole genome shotgun sequence.
ACCESSION   DAOQVY010000083 DAOQVY010000000
VERSION     DAOQVY010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10125
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7443_length_10125_cov_5.325621"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PKN91_08545"
     CDS             <1..972
                     /locus_tag="PKN91_08545"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNS28161.1"
                     /translation="AIVGQWQRPNGLLIQRVRVPLGVIGIIYESRPNVTADAGALCLK
                     SGNPVILRGGSESSRSNRAIHACLAAGLAAADLPVEAIQLVPTTDREAVGCMLGGMRE
                     YIDVLVPRGGKALVARVKAEARVPVIGHLEGVCHVYVDRDADADMARAIVHNAKLRRT
                     GVCGAAETLLVDRAAGAGLLTMLVRDLLDAGCEVRGDEMMRAVDPRVRPADEADWAAE
                     YLDAVIAARVVDGVDAAIAHIARYGSSHTEAIVTANAATAERFLSRVDSAIVLHNAST
                     QFADGGEFGMGAEIGISTDRFHARGPVGVEQLTSYKYVVRGGGQTRA"
     gene            complement(957..2564)
                     /locus_tag="PKN91_08550"
     CDS             complement(957..2564)
                     /locus_tag="PKN91_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNS28162.1"
                     /translation="MPHFRLTSASIDVAAAQRELADPAFGGYVAFEGWVRDFNEGRRV
                     EHLEYEAFEPLAVREGERILAEACERHGVTHALCIHRIGDLAIGELAVWVGVSAPHRD
                     EAFRACRQIIDEVKHRVPIWKKEHYEDGDSGWVNCERCAAPAPDPAGPAPAVPHDHDH
                     SHGHDRHVHTHADHAARVPAPDYSRQMALREVGAAGQARLAAASVLVVGAGGLGVPVL
                     QYLAAAGIGRLGIVDHDRLEPSNLHRQTMYSLADCGRPKAQLAAARLRELGAARIDVH
                     VARLDASNVTPLLTPYDIVVDCTDNFATKLLLNDAALALARIAVFASVYQYEGQLQVV
                     DPGRGTACLRCVWPAATRDGLVGNCATAGVLGPVPGTLGTLQALEVLKVLLDLPGRLG
                     GEVMLIDLLSLATRRLQARPAADHHSHGRQPAESTAADIDLSFESIAAAATAGYTIVD
                     IREPAETAVSPIVGAPVQELPLGALLSGAPLAPGERHLLVCAHGMRSHAAAEALRARG
                     LRQVYSLRGGTAALAQAGLRRGAQARV"
     gene            complement(2576..3418)
                     /locus_tag="PKN91_08555"
     CDS             complement(2576..3418)
                     /locus_tag="PKN91_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNS28163.1"
                     /translation="MNAAAPLHGLVLTGGRSTRMKRDKAALAYHGETQLEHAWRLLAP
                     HVARRFVSVRADQRHDPLRAGYPQIIDSIADAGPAAGILAAFEAHPDAAWLVLACDLP
                     HLDARTLGHLVAQRDPTRPATAYRSAHDGLPEPLCAIWEPASRAPLTAALAAGRSCPR
                     KFLINADTKLLDPLVPHALDNVNESHEYWSAMSSLNAAPAPLHVKVQYFALLREQAGR
                     SEETLATLARTPRDLYEELRARHSFTLPPELLRVAVNAEFGDWTQPLADGDAVVFIPP
                     VAGG"
     gene            complement(3415..3915)
                     /gene="moaC"
                     /locus_tag="PKN91_08560"
     CDS             complement(3415..3915)
                     /gene="moaC"
                     /locus_tag="PKN91_08560"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNS28164.1"
                     /translation="MATRKAAPALTHVDAARRPTMVDVGTKDITLREASAEAIVTLPR
                     AAALALRRGKERTKKGPVFDTAIVAGVMAAKRTHELIPFCHPLGLERCRITIEQPRAG
                     RIVIRCDVAVHGRTGVEMEALTGAVGAALTIYDMTKALSHDIVIESVRLVSKSGGKHD
                     FRRARR"
     gene            complement(3928..4983)
                     /gene="moaA"
                     /locus_tag="PKN91_08565"
     CDS             complement(3928..4983)
                     /gene="moaA"
                     /locus_tag="PKN91_08565"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNS28165.1"
                     /translation="MAPADSPIPIARARGLRDALSRPLHDLRISVMDRCNFRCTYCMP
                     RETFHERYRFLKSAERLSFDEIVRIARLFVSLGVRKLRITGGEPLLRPNLADLVGDLT
                     TLAGVEDVALTTNGVLLAKYASELKAAGLGRVTVSLDSLDPQVFRAMSGDFDELDAVL
                     EGIEHARRAGLTPIKVNSVIQRGINDAGVLDLAARFRGTGVSVRFIEYMDVGNRNHWT
                     PDLVVPSAELHARIHARWPLVPRGRSYVGEVAEHYAYADGEGEIGFISSVSQPFCGDC
                     SRARLSSDGGLYTCLFATHGLDLRAPLRAGATDEELLAIIRGRWAQRTDRYSELRATE
                     RAAAAQGRKVEMNYIGG"
     gene            complement(5081..5440)
                     /locus_tag="PKN91_08570"
     CDS             complement(5081..5440)
                     /locus_tag="PKN91_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28166.1"
                     /translation="MKLPILAAALVIAFAGGPVYAACTYPTSPDKIPDGRVASMEEMI
                     AAKKQVEQYNKDMEAYLSCIKLEFDAQSADSTLTAEQKTELEKRQVQKHNAAIDELEG
                     VAARFNEQVKAYKAAHP"
     gene            complement(5543..6199)
                     /locus_tag="PKN91_08575"
     CDS             complement(5543..6199)
                     /locus_tag="PKN91_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28167.1"
                     /translation="MKSLLPTLLTAALAATLPGLAAAGTQHAAAVTPPTAAAVPATVV
                     ATRNALRDLWIGHIFWVRNVVVARYDDSAAARTAEAQVVANAKAIAGAIEPFYGKAAS
                     DKLFGLLAGHWGAISEHIDATEQGSKSGQEAATGKLLANANEIADFLAAANPNWPVDT
                     LRGLLSAHGAHHLQQDQQLHDGQYDAEARTWAAMSAHMYVVADALADGLAAQFPDRFR
                     "
     gene            6279..6917
                     /locus_tag="PKN91_08580"
     CDS             6279..6917
                     /locus_tag="PKN91_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HNS28168.1"
                     /translation="MPDLFGDRRRRLLRHLLRNKAGASVDELAQALGVTRTAVRQHLA
                     ALTRDGLVAAGAERGTGGRPVRLHVLTEAGREVFPRRYSWFANLLVEAIAREHGASGL
                     RTRLGRIAAAVVAQLPEPAGAGAGRRDKVEELSALMDQLGYDARRVRDVGGVPTIEAD
                     NCVFHALAMQNTAVCHFDLALMTSYTGRKVELHECMAKGGKVCRFRFLQSDA"
     gene            6952..7028
                     /locus_tag="PKN91_08585"
     tRNA            6952..7028
                     /locus_tag="PKN91_08585"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6986..6988,aa:Arg,seq:tcg)
     gene            7128..8216
                     /locus_tag="PKN91_08590"
     CDS             7128..8216
                     /locus_tag="PKN91_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNS28169.1"
                     /translation="MLISAAVSRAADESPVITELELDDPRDDEVLVRLVATGICHTDL
                     FAPQLVPLPAVFGHEGAGIVERVGARVGKLRAGDRVAMTFGSCGRCRHCHDAAPAYCE
                     HGHHLQFGGTREDGSTTLSCAGERIHGSFFQQSSFATYALATERNVVRLPDLMPIELA
                     GPLGCGVQTGAGAVLNTLHAPAGSSLAVFGTGAVGLSAIMAARYANLGVIIAVDIDPG
                     RLELARELGATHAIDAREGDVAARIHGIARGGAMYAIETAGALASLEASIECLARRGV
                     CALVTVPNLGAPVPFSLLPLLTGGRRLIAVLEGDSIPDLFIPRLAELHLAGKLPIDRL
                     VRDYAFADVARAMHDAASGRAIKPVLRF"
     gene            8330..8791
                     /locus_tag="PKN91_08595"
     CDS             8330..8791
                     /locus_tag="PKN91_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNS28170.1"
                     /translation="MQTQPASSADLALRDELAQFVQRFVAGYQSGDVEQMRPLYAPSA
                     MIWAHNRPTAIGWPGIADFFAHSFAKYRAKVDAHVLDVERMAEGRVVLYTLARVDLEP
                     KAPGSQPVTAYFRDVIILVRSAEGWQIETNVDQPTTRELFEADRARVPLRH"
     gene            complement(8809..9243)
                     /locus_tag="PKN91_08600"
     CDS             complement(8809..9243)
                     /locus_tag="PKN91_08600"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNS28171.1"
                     /translation="MTDEKTHDGSCFCGAVQFTVTGEPAAMGYCHCESCRTWSAGPVN
                     AFTLWPPAALRITRGADGIGAYSKTPASIRKWCRNCGGHLFTEHPQWNLVDVYAALIP
                     GFPYQAGVHVHYQETRLPMKDGLPKFKDIPAEMGGSGTVLAE"
     gene            complement(9314..9628)
                     /locus_tag="PKN91_08605"
     CDS             complement(9314..9628)
                     /locus_tag="PKN91_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HNS28172.1"
                     /translation="MSTQRRSVTLGHIVEEEDALSVDDLSRLCAVERSFILELVQEGV
                     LEVQPATEARFVGRSLRRARLAVRLQRDLGVNVAGAALVIELLERIETLERREENLPG
                     VG"
     gene            complement(9625..10080)
                     /locus_tag="PKN91_08610"
     CDS             complement(9625..10080)
                     /locus_tag="PKN91_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNS28173.1"
                     /translation="MGDRTLNVKIPKGIQAGQHIRLAGQGGQMPGEARPGDLLLEVSF
                     APHPHFRPEGRDLYLDLPVAPWEAALGASVTVPTPEGPVSLQVPPGSRAGTRLRLRGR
                     GLPARPPGDLYATLQIALPPADTAAARAAYEAFAAGAPFNPRSSLGAGS"
BASE COUNT         1502 a         3364 c         3629 g         1630 t
ORIGIN      
        1 gccattgtcg ggcagtggca gcggccgaac ggcctgctga tccaacgggt gcgcgtgccg
       61 ctcggcgtga tcggcatcat ctacgagagc cggccgaacg tgaccgcgga tgcgggggcg
      121 ctgtgcctca agtccggcaa tccggtgatc ctgcgcggag gctcggagag cagccgctcg
      181 aaccgcgcga tccatgcctg cctggcggcg ggcctcgccg ccgcggacct gccggtcgag
      241 gcgatacagc tcgtgcccac caccgatcgc gaagcggtgg gctgcatgct cggcggcatg
      301 cgtgaataca tcgacgtgct ggtgccgcgc ggcggcaagg ccctggtcgc gcgggtgaag
      361 gccgaggcgc gcgtgccggt gatcgggcac ctggaaggcg tgtgccacgt ctatgtcgat
      421 cgcgatgccg atgcggacat ggcgcgcgcg atcgtgcaca acgcgaagct gcggcgcacg
      481 ggagtgtgcg gcgcggccga gacgctgctc gtcgatcgag ccgcgggcgc cggcctgctg
      541 acaatgctgg tgcgcgacct cctcgatgcc ggttgcgaag tgcgcggcga cgagatgatg
      601 cgggcggtgg atccgcgcgt gcggcccgcc gacgaagccg actgggccgc cgagtacctg
      661 gacgcggtga tcgcggcgcg tgtcgtcgac ggcgtcgatg cggcgatcgc gcacatcgcg
      721 cgctacggtt cgtcgcacac ggaggcgatc gtcacggcca acgccgcgac cgcggagcgc
      781 ttcctgtcgc gagtcgacag cgcgatcgtg ctgcataacg cctccacgca gttcgccgac
      841 ggcggcgagt tcggcatggg cgcggagatc ggcatttcca ccgatcgctt ccacgctcgc
      901 ggcccggtgg gcgtggaaca gttgacgagc tacaagtacg tggtgcgcgg cgggggtcag
      961 acccgcgcct gagcgccccg gcgcagccca gcctgcgcca gcgccgcggt accaccgcgc
     1021 agcgaataca cctgccgcaa cccgcgcgcc cgcagcgcct cggccgcggc atggctgcgc
     1081 atgccgtggg cgcagacgag caggtgccgc tcgccgggcg ccagcggggc gccggagagc
     1141 agcgccccga gcggcaattc ctgcacggga gccccgacga tcgggctcac ggcggtctcc
     1201 gcgggttcgc ggatatcgac gatcgtgtag cccgccgtcg ccgcggccgc gatcgattcg
     1261 aaactgagat cgatgtcggc agcggtgctc tcggcgggct gccgcccgtg gctgtggtga
     1321 tccgcggccg gccgcgcctg caggcgtctg gtggcgagcg acaacaggtc gatcagcatc
     1381 acttcgccgc cgaggcggcc cgggagatcg agcaggacct tcagcacttc gagcgcctgc
     1441 agcgtaccga gcgtgcccgg caccgggccg agcacgccgg cggtcgcgca attgccgacc
     1501 aggccgtcgc gcgtggcggc gggccaaacg cagcgcaggc aggcggtgcc gcgcccggga
     1561 tcgaccacct gcagctgacc ctcgtactgg tagacgctcg cgaacacggc aatgcgcgcg
     1621 agcgcgaggg cggcgtcgtt caacagcaac ttcgtcgcga agttgtcggt gcagtcgacg
     1681 acgatgtcgt agggcgtgag cagtggcgtg acgttcgagg catcgaggcg cgcgacatgg
     1741 acatcgatcc gcgccgcacc cagctcgcgc agccgggccg ccgccagctg cgctttcggc
     1801 ctgccgcagt cggcgagcga atacatcgtc tggcggtgca ggttcgaggg ctcgagcctg
     1861 tcatggtcga cgatgccgag gcgcccgatg cccgcggcgg ccaggtactg cagcaccggc
     1921 acgccgaggc cgccggcgcc gaccaccagt accgacgcgg ccgcgagacg cgcctggccg
     1981 gcggcaccga cttcgcgcag cgccatctgc cgcgagtaat ccggcgcggg cacgcgcgcg
     2041 gcgtgatcgg catgtgtgtg gacgtggcgg tcgtgaccgt gactgtggtc gtggtcgtga
     2101 ggcacggcag gcgcaggtcc cgccgggtcg ggagcgggcg ccgcacagcg ctcgcagttc
     2161 acccagcccg agtcaccatc ctcgtagtgc tccttcttcc agatcggcac gcggtgcttg
     2221 acctcgtcga tgatctggcg gcacgcgcgg aaggcctcgt cccggtgcgg tgcgctgact
     2281 ccgacccaca cggccagttc gccgatggcg aggtcgccga tccggtggat gcatagggca
     2341 tgcgtgacgc cgtgccgttc gcaggcctcg gcgaggatgc gctcgccttc gcgcacggcc
     2401 agtggctcga acgcttcgta ctcgaggtgc tcgacgcggc ggccttcatt gaagtcgcgg
     2461 acccagccct cgaaggcgac gtagccgcca aaggcggggt cggcaagttc gcgctgcgcg
     2521 gcggcgacgt cgatcgaagc gctggtgagg cgaaagtgcg gcacggcggg cctcgtcagc
     2581 cgcccgcgac cggcggaatg aacaccaccg cgtcaccgtc ggcaagtggt tgtgtccagt
     2641 cgccgaactc cgcgttcacg gccacccgca gcagttcggg cggcagcgtg aacgaatggc
     2701 gcgcgcgcaa ttcctcgtag aggtcgcgcg gtgtgcgcgc gagcgtggcg agtgtctctt
     2761 cactgcgtcc ggcctgctcg cgcagcagtg cgaaatactg cactttgacg tgcagcgggg
     2821 ccggtgcggc gttcaatgag ctcatggccg accaatactc gtggctttca ttcacgttgt
     2881 cgagcgcgtg cgggacgagc ggatcgagca gcttggtgtc ggcattgatc aggaacttgc
     2941 gcgggcagct gcgccccgcg gcgagcgcgg cggtgagcgg cgcgcggctg gccggctccc
     3001 agatggcgca caggggctcg ggcaggccat cgtgtgcact gcggtaggcg gtggcaggtc
     3061 gcgtgggatc gcgttgcgca accagatggc cgagcgtgcg cgcatcgagg tgcggcaggt
     3121 cgcaggcgag caccagccat gccgcgtccg gatgcgcctc gaacgcggcg aggatgccgg
     3181 cagccggccc cgcgtctgcg atcgaatcga tgatctgcgg atagcccgcg cgcagcggat
     3241 cgtgccgttg gtcggcgcgc accgacacga aacgccgcgc cacatggggt gcgagcaggc
     3301 gccacgcgtg ctcgagctgc gtttcgccat ggtaggcgag ggcggccttg tcgcgcttca
     3361 tgcgcgtgct gcgcccgccg gtgagcacca ggccatgcag cggcgccgcc gcgttcatcg
     3421 ccgtgcgcgc cggaagtcgt gcttgccgcc gctcttcgat accaggcgca cgctttcgat
     3481 cacgatgtcg tgcgagagcg ccttggtcat gtcgtagatc gtgagggcgg cgccgacggc
     3541 gccggtgagc gcttccatct ccacgccggt gcgcccgtgt accgccacgt cgcagcggat
     3601 gacgatgcgg cccgcacgcg gctgctcgat cgtgatgcgg cagcgctcga gcccgagcgg
     3661 atgacagaac gggatgagct cgtgggtgcg ctttgcggcc atcacgccgg cgacgatcgc
     3721 ggtgtcgaag accggtccct tcttggtgcg ctccttgccg cggcgcaggg ccagcgccgc
     3781 cgcgcgcggc agcgtcacga tggcctcggc gctcgcctcg cgcagtgtga tgtccttggt
     3841 gccgacatcg accatggtcg ggcggcgcgc ggcatccaca tgcgtcagcg cgggcgcggc
     3901 tttgcgggtg gccatagtcg cgcgttccta gccgccgatg tagttcattt cgaccttgcg
     3961 cccctgcgct gcggcggcgc gctccgtggc gcgcaactcg ctgtagcgat cggtgcgctg
     4021 ggcccagcgc ccgcggatga tggcgagcag ctcctcgtca gtcgcaccgg cgcgcagcgg
     4081 cgcgcgcagg tcgagtccgt gggtcgcgaa caggcaggta tagagtccgc cgtcggacga
     4141 caggcgcgca cgcgagcaat cgccgcagaa gggctgcgat accgaggaaa tgaagccgat
     4201 ctcgccctcg ccgtcggcgt aggcgtagtg ctcggccact tcgcccacgt agctgcggcc
     4261 acgcggcacg agcggccagc gggcgtggat gcgcgcgtgc agttcggcgg agggcacgac
     4321 gagatcgggc gtccagtggt tgcgattgcc gacgtccatg tattcgatga agcgcacgct
     4381 gacgccggtg ccgcgaaagc gcgcggcgag gtcgagtacg ccggcatcgt tgatgccgcg
     4441 ctggatcacc gagttgacct tgatcggggt caggccggcg cggcgtgcat gctcgatccc
     4501 ttcgagcacg gcgtcgagct cgtcgaagtc gccgctcatg gcgcggaaca cctgcggatc
     4561 gaggctgtcg aggctcacgg tcacccgccc gaggcccgcg gccttcagct cgctcgcata
     4621 cttcgcgagc agcacgccgt tggtggtgag cgccacgtcc tcgacaccgg caagcgtggt
     4681 caggtcaccg acgagatcgg cgagattcgg gcgcagcagc ggttcgccgc ccgtgatgcg
     4741 cagcttgcgc acgccgagcg aaacgaacag gcgcgcaatg cgcacgatct cgtcgaacga
     4801 caggcgctcg gcactcttca ggaagcggta gcgctcatgg aaagtctccc gcggcatgca
     4861 ataggtgcag cggaagttgc accgatccat gaccgagatg cgcaggtcgt gcagcggccg
     4921 cgagagcgcg tcgcgcagac cgcgcgcgcg cgcgatcgga attggggagt cggccggggc
     4981 catacgaata tcttcgctcc cgaagtcgcg caggtacagc tatccgatgc gccgggtcga
     5041 tccgtgagtg caggtcgacc cggcgcgggg cgggtatcaa tcagggatgc gcggccttgt
     5101 aggccttcac ctgctcgttg aagcgggcgg cgacgccctc gagctcgtcg attgcggcat
     5161 tgtgcttctg cacctggcgc ttctcgagtt cggtcttctg ctcggcggtg agcgtgctgt
     5221 cggcgctctg cgcgtcgaat tcgagcttga tgcacgacag gtaggcttcc atgtccttgt
     5281 tgtactgctc cacctgcttc ttggcggcga tcatttcctc catcgacgcg accctgccgt
     5341 ccgggatctt gtccgggctt gtcgggtaag tgcacgcggc atagacgggg ccaccggcga
     5401 aggcaatgac gagcgcggcg gcaagaatcg gcaacttcat gaatgggatc ctcgggtcgg
     5461 aataggggtc gggactggca gcgaccccta tattagggcc agactcctcg ccggggccac
     5521 tttctgcccg ttcagcctgc gttcagcgaa agcggtccgg aaactgcgcc gccagcccgt
     5581 cggccagagc atcggcaact acgtacatgt gggccgacat cgccgcccag gtgcgcgcct
     5641 cggcgtcgta ctggccatcg tgcagctgct ggtcctgctg aaggtgatgg gcgccgtgcg
     5701 ccgacagcag gccgcgcagc gtatccaccg gccagttggg attggcggcg gcgaggaaat
     5761 cggcgatttc gttcgcgttg gcgagcagct tcccggttgc cgcttcctgg cccgacttcg
     5821 agccctgttc cgtggcatcg atgtgttcac tgatcgcccc ccagtggccg gcgagcagcc
     5881 cgaacagctt gtccgaggcg gccttgccgt agaagggttc gatcgcgccg gcgatagcct
     5941 tcgcattcgc gaccacctgc gcctccgcgg ttctggcggc agcactgtcg tcgtagcggg
     6001 cgacgaccac gttgcgcacc cagaagatgt gaccgatcca caggtcgcgc agcgcgttgc
     6061 gggtcgcgac caccgtggcc ggtacggcgg cggcggtcgg gggcgtgacc gcggccgcat
     6121 gctgcgtgcc agcggcggca agtcccggca gggtggcggc gagtgcggcg gtgagcagag
     6181 tcggcaacag cgatttcatc ggagtcctcc aggtgaccgt gtgtgcattc cgttctacgc
     6241 tggaaaaaac acaaaagaaa tattaatatg ccaaaagtat gcccgacctc ttcggtgatc
     6301 gccgccgccg gttgttgcgc cacctgctca ggaacaaggc gggcgccagc gtcgatgagc
     6361 tggcgcaggc gctgggtgtg acccgcaccg cagtgcgtca gcacctcgct gcgctgacgc
     6421 gcgacggcct ggtcgccgcc ggtgccgagc gcggcacggg cgggcgtccg gtgcgcctgc
     6481 acgtgctgac cgaggcgggc cgcgaggtgt tcccacggcg ctattcgtgg ttcgcgaacc
     6541 tgctcgtcga ggcgatcgcc cgcgagcacg gcgcgagcgg gctgcgcacg cggctcggac
     6601 gcatcgcggc ggccgtggtc gcgcaattgc ccgagccggc gggcgcgggc gcgggccggc
     6661 gcgacaaggt cgaggagctc tcggcgctca tggaccagct gggctacgac gcgcgccgcg
     6721 tgcgcgacgt cggtggcgtg ccgacgatcg aagccgacaa ctgcgtgttc cacgcgctcg
     6781 ccatgcagaa caccgcggtc tgccacttcg acctcgcgct gatgaccagc tacacgggtc
     6841 ggaaggtcga gctgcacgag tgcatggcca agggggggaa ggtgtgccgg ttccgcttcc
     6901 tgcagagcga cgcgtgactc cgtcgcgcgc gccatgcata ttgcgcgtca cgcgcccgta
     6961 gctcagctgg atagagcatc aggcttcgaa cctgagggtc gggagttcga atctctccgg
     7021 gcgcgccatc cgcgcagagg gtaggaaatc atggtgtcgg ccatcatcgg agcgggcctg
     7081 aacgatccgc aggcgcagaa cgtggcggcg agcacgcccg gcccagcatg ctgatttctg
     7141 ccgcggtctc gcgcgcggcc gacgagtcgc cggtcatcac cgagctcgaa ctcgacgacc
     7201 cccgtgacga cgaggtcctc gtgcgcctgg tcgcgaccgg gatctgtcac accgatctgt
     7261 ttgcgcccca gctcgtgccg ctgcccgcgg tgttcgggca cgaaggggcg ggcatcgtcg
     7321 agcgcgtggg ggcgcgggtc gggaagctgc gtgcgggcga tcgggtcgcg atgaccttcg
     7381 gctcctgtgg ccgctgtcgg cattgccacg atgcggcgcc ggcctactgc gaacatggcc
     7441 atcacctgca gttcggcggc acgcgggagg atggcagcac gactctgtcg tgcgcgggcg
     7501 agcgcatcca cggctcgttc ttccagcagt cttcgtttgc aacgtacgcg ctggccacgg
     7561 agcgcaacgt ggtgcggctg cctgacctga tgccgatcga gttggccggc ccgctcgggt
     7621 gcggcgtgca gaccggtgcg ggtgccgtgc tgaacacatt gcacgcgccg gccggcagct
     7681 ctcttgcggt tttcggcacg ggcgccgtgg gcctctccgc gatcatggcg gcgcgttatg
     7741 cgaatctcgg cgtgatcatc gccgtcgaca tcgatcccgg gcggctcgaa ctcgcccgcg
     7801 agttgggcgc cacgcacgcg atcgacgccc gcgagggaga tgtcgccgcg cgcatccacg
     7861 gcatcgcgcg cggcggggcc atgtatgcca tcgaaaccgc gggcgcgctg gcctcgctcg
     7921 aggcgtcgat cgagtgcctg gcccggcgcg gcgtgtgtgc gctggtgacc gtaccgaatc
     7981 tcggtgcgcc cgtgccgttc agcctgctgc cgctgctcac cggcggcagg cgactgatcg
     8041 cggtgctcga aggtgacagc attccggatc ttttcatccc ccggctcgcc gaactccatc
     8101 tggcggggaa gctgcccatc gatcggctgg tgcgggatta cgctttcgcc gacgtcgcgc
     8161 gtgcgatgca cgacgcggcc agcggtcggg ccatcaaacc cgtccttcgc ttctaactgc
     8221 gaatcacgga gcctaaacga tgcgatcgat actcgagtcg aaagctgcat gcactgtgtc
     8281 tctgctgctt gcgcttccgt tggctgcttg cgcgacagag ccgctcgcgg tgcagacaca
     8341 gccggcgagt tcggcagacc tggcgctgcg cgacgaactg gcgcagttcg tgcagcgctt
     8401 cgtcgccggc taccaatcgg gcgatgtgga acagatgcgg ccgctctatg caccatcggc
     8461 catgatctgg gcccataacc gcccaacggc gatcggctgg ccggggatcg cggatttctt
     8521 cgcgcattcc ttcgcgaagt accgggccaa agtcgacgcg cacgtgctcg acgtggagcg
     8581 catggctgaa ggacgcgtgg tcctctatac gctcgcgcgc gtggatctcg agccgaaggc
     8641 tcccgggtcg cagccggtca ccgcgtattt ccgcgatgtc atcatcctcg tgcgcagcgc
     8701 ggaaggctgg cagatcgaga cgaacgtgga tcagccgaca accagggagc tgttcgaggc
     8761 ggaccgggcg cgcgtgccgt tgcgccatta gcgcgcaacg cgcggtcgct actcggcgag
     8821 cacggtaccc gagccgccca tttcggccgg tatgtccttg aacttcggca ggccgtcctt
     8881 catcggcagg cgcgtctcct ggtagtgcac gtggacgccg gcctggtacg gaaagccggg
     8941 aatcaacgcc gcgtagacat cgacgagatt ccattgcggg tgctccgtga acaggtgccc
     9001 gccgcagttt ctgcaccact tgcgaatgct ggccggcgtc ttgctgtatg caccgatacc
     9061 gtcggcgccg cgagtgatgc gcagcgcggc cggcggccag agcgtgaagg cattgaccgg
     9121 cccggccgac catgtgcgac aggattcgca gtggcagtag cccatcgcgg cgggctcccc
     9181 ggttaccgtg aactgcactg cgccgcagaa gcagctgccg tcgtgtgttt tttcgtctgt
     9241 catccctgac tccgatcatt tcattggaca ttgttggcat gccggacggc gggccgccat
     9301 tggccggcac ttgtcacccg acgccgggga ggttttcctc gcgtcgttcg agcgtttcga
     9361 tgcgctcgag caattctata acgagcgcgg ccccggcgac attcacgccg aggtcgcgct
     9421 gcagccgcac cgcgagccgc gcgcgtcgca gcgagcgccc gacgaatctc gcctccgtcg
     9481 cgggctgcac ttcgagcacg ccttcctgca ccagttcgag gatgaacgat cgctccaccg
     9541 cgcagaggcg actgaggtcg tccacgctga gggcatcctc ttcttcgact atgtgaccca
     9601 gggtcaccga ccgcctctgc gtgctcatga tcctgctcca agcgacgacc tgggattgaa
     9661 cggcgccccg gccgcgaaag cctcataggc tgcgcgcgcc gccgcggtat cggccggcgg
     9721 cagcgcgatc tgcagcgtgg catagagatc gcccggcggc cgcgcgggca ggcctcgacc
     9781 gcgcaggcgc aggcgggtgc ctgcccgcga accgggtgga acctgcagag aaaccgggcc
     9841 ctcgggcgtg ggtaccgtca ccgatgcgcc gagcgccgcc tcccaggggg cgaccggcag
     9901 gtccaggtac aggtcgcgtc cctcggggcg gaagtggggg tgcggcgcaa agctcacttc
     9961 gagcagcaag tcgccgggcc tggcttcgcc gggcatctgc ccgccctggc cggcgagccg
    10021 gatgtgctgc ccggcctgga tgcctttggg aattttcacg ttgagggtgc gatcgcccac
    10081 cgtcagcgtg cgcgtgccgc cttacagcga tgccgccagg tcgac
//
LOCUS       DAOQVY010000084        10077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_7496_length_10077_cov_5.926462, whole genome shotgun sequence.
ACCESSION   DAOQVY010000084 DAOQVY010000000
VERSION     DAOQVY010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10077
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7496_length_10077_cov_5.926462"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2449)
                     /gene="glgP"
                     /locus_tag="PKN91_08615"
     CDS             complement(<1..2449)
                     /gene="glgP"
                     /locus_tag="PKN91_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNS28174.1"
                     /translation="MTSSIFIEITAKIPEKLQRLPELAGNLFFSWHRPVRSLFEDLDA
                     GLWAQTSGNAKLLLRCVGQEALDRAAADPVYVARYGQVLATFDAYLAQAAAPSDAPLV
                     AYFCAEYGFHESFPIYSGGLGILAGDHCKAASDERLNFVAIGLMYGQGYFVQTVDSDG
                     VQHAVYQDHDPRDLPVEPVRNADGTWLRVTIDIAGRPVHARLWSAQAGRVPVYLLDTN
                     CEDNAPVDRDITHRLYGGDRVTRIRQEMVLGIGGVRALRALGLAPAVWHLNEGHAAFL
                     ILERMREEVRNGLPADVALEAVAAQCAFTTHTPVAAGHDSFPHDLVREQFSSYLDSLG
                     WPADRVLALGRAPDAPHEFNMTWLALAGTRHVNGVSRVHRGESAKLCAKHWPELTAQE
                     NPVGFVTNGVHVPTFLHQTWAEFFDQHLSRDWLGRLSDSSLWATLEHVPDKAFWVAAQ
                     DVKSRMFAMVRERLKAEFATKGMSGAQWRQTSRWIDPERPDVLTIGFARRFATYKRAS
                     LLLRDRARLARLVNDSARPMVLLFAGKAHPADEPGKAVLREIKQLMLTPEFAGRVLFL
                     DDYDMQLGRALVSGCDVWLNNPIAPLEASGTSGIKAATNGTLNLSILDGWWTEAWDGE
                     NGWGLSPVPVQDAARRDALEAENVLDTLEEEVLPLYYSRNGSGFPVEWVRKSKHAMAT
                     VIPAFNMGRVVNDYADGIYRPAARLGAALAADGYAGARALTEFKQRVRAAWPRVALVR
                     VSDPPPQIDLFGRLQMRVAVQLAGLAPDEVAVELVGKRRLPRPPDDEPLLTSYREQPR
                     DGHLNLAFTPTGETTGEG"
     gene            2569..2916
                     /locus_tag="PKN91_08620"
     CDS             2569..2916
                     /locus_tag="PKN91_08620"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNS28175.1"
                     /translation="MDDSSARKFQKDLNGGLVALVLLSVLDQARGELYGYEIAKQLQR
                     AADGEPLFKEGTIYPVLRSLSASGLLSSRIEPSLSGPPRKYYRITDSGGMVLGEWRAI
                     WQQTRNFVDRFTS"
     gene            2928..3833
                     /locus_tag="PKN91_08625"
     CDS             2928..3833
                     /locus_tag="PKN91_08625"
                     /inference="COORDINATES: protein motif:HMM:NF025168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HNS28176.1"
                     /translation="MNRTAPRSIDAYLKQLREALADADPSLVQDALYDAEEYLRAELG
                     AHPGKSESDVLELIASTYGAPDEVAAAYRDTEVKVAAALHTPRPAPGGGAWRRFFSVY
                     SDPRAYASLFYLFLALALGIFHFTFTVVGLSLSAGLAILIIGVPFFLAFIGLTRVIAL
                     AEGRLLEAVSGERMPRRPLHPGAKLSFWTRILEMLRDARTWTTIAYLILALPLGIIYF
                     TIAVTGMALGLSLTFAPVFEIGRRLGWFDVAADIHFSPAWLASPWLLPLAIALGVVII
                     TLLMHAARFIGRVHARFAKHMLVIG"
     gene            3833..4444
                     /locus_tag="PKN91_08630"
     CDS             3833..4444
                     /locus_tag="PKN91_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28177.1"
                     /translation="MRVKWPAPALALVMAACSASDVPDAGPAAEQAAAATAGCLASGD
                     GYLRARIRGALDLDIDWRNGDIECTGGPRPDGSGLRVTFAGPEQSDGRRLRFVFGIAQ
                     AAEGIAAQGLPVNLTVIFEGERRLFATQGDDKCTVDALTQQRTGPLGGGSRSWRIEAR
                     GFCTEPASTLQGDERLLVTRFDFAGKIDIEGESPFTGTESHAS"
     gene            4434..4919
                     /locus_tag="PKN91_08635"
     CDS             4434..4919
                     /locus_tag="PKN91_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNS28178.1"
                     /translation="MQAEIRRSRSAARAPLLAAAALLAVLANADTGLEDLDAFPRASV
                     EITSGERVHRFDVWVADTPERQAQGLMYVRDLDAGSGMLFVHAAPREVSMWMKNTYIP
                     LDMLFIDRGGRIVRIAHETTPHSLDSIRSGTPVTRVLEIRGGEAARRGIAVGDRVRTR
                     S"
     gene            5057..6268
                     /locus_tag="PKN91_08640"
     CDS             5057..6268
                     /locus_tag="PKN91_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNS28179.1"
                     /translation="MNNNVSLTCVAAALLLASLPAAAAPPAPSWYIAPEAIYMWADRD
                     RLADDEIGGQIAFGRSFSDKWDAELAYSRVSTDALGNSGLDIDSVFLNLHRVFYRDSR
                     ISPFLKLGYGYVDADYGGGALAGEKVESWTLAYGLGLLANLKVDTAQGTALALRAEIY
                     GLYSTETAPPNRDHLNDTVAGIGLQYQWGAPVVVPVAAPVIGDSDGDGVNDDLDRCPG
                     TPSGTPVDARGCELDSDGDGVVDRLDKCPGTPAGAKVDANGCELDGDGDGVVDRIDQC
                     PNTRAGEKVDEVGCSFALRLEVYFDTASAEIKPESFRDLDLAVSLMQRNQTVRGTIEG
                     HTDDRGTEAYNLELSKRRAASVRQYLIDKGVAASRLESVGYGESRPEADNATAEGRAQ
                     NRRVVLVRSDR"
     gene            6352..6957
                     /locus_tag="PKN91_08645"
     CDS             6352..6957
                     /locus_tag="PKN91_08645"
                     /inference="COORDINATES: protein motif:HMM:NF016974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HNS28180.1"
                     /translation="MLDLPGQPCIDKSRLIGGCIRLPLAVDAARLAAEVAALPASLWG
                     TQGGRVGVHRPAEAIFLRGHAPAEGELPIDDRAPLALLPYAREIVTTLIAAPVQRCLL
                     ARLPGGAVIDLHIDQAPYFAKTIRIHVPVVTHDEVWMLAGGRRYRMAAGEVWALDNSA
                     PHGVRNGHATLSRTHLIADFLPSPPLLALLAKGARGLGLAG"
     gene            6954..7643
                     /locus_tag="PKN91_08650"
     CDS             6954..7643
                     /locus_tag="PKN91_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28181.1"
                     /translation="MILTPRFVFLHLHKSGGSFVNEALLRHEAGASQYGYHLPRSAIP
                     ASHARLPVLGLVRNPWSYYVSWYAFQRARPRPNALFGVLADGGRADFAATIRNMLSLG
                     EHDALLDRVIAALPAQYANHGLNLPGPALAAIRGTGLGFYSWLYQYLYGAPVGLHIAR
                     MEVLRTELPQVMAEAGVPAGAALGEFLRTSAPRNVSAHGPAADYYDADLRALVGRRDA
                     ALVERYGYVAD"
     gene            complement(7565..9451)
                     /locus_tag="PKN91_08655"
     CDS             complement(7565..9451)
                     /locus_tag="PKN91_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNS28182.1"
                     /translation="MRLPQPFQRLAVRFDAQRLREEVEALPADAWVSHPDGIPGNSAL
                     RLISVDGGENDAVSGRMLATPQLARMPYLRQVLASFGVVWSRSRLLRLAPGASVPEHA
                     DINHHWFSRVRLHVPIVTEPEVRFHCGGESVHMATGEAWIFDTWRLHRVEHGGTQPRI
                     HLVADTSGSAAFWQFVQQSATRQTGSQDPGAPMLQFDPGRDAMPLTEQAPEPCVMSPG
                     ELELLVLDLRAELAALEDSPGAATRLARYHALLDALCRDWRQLYALHAQSAPGWTEFE
                     RLLDVVRARAQESGAGLVMATNRVASNQVLEARVLRPALSLARVIARRPARPVFIIAA
                     PRSGSTLLFETLAASHDVATLGGEAPWLIEGLAALRPGAPGVESNRLTAEHASDAIAA
                     RIITELAERRVDAAGRRVADDAPLRFLEKTPRNALRVPFINRLFPDAQFIFLWRDPHG
                     NLASIIEAWEAGGWKTYNGLDGFDGPWSLLLPPGWRTMRGRPLAEIAAFQWESANRIA
                     LEDLEQLPHARWTAVSYEDLVADPRATIERLCRFAGIEFDAALEIRVQGALPLSRYTH
                     TAPAPDKWRRREAEVLGVLPSITSVWDRLRRLEPDGFQSATYPYRSTSAASRRPTSAR
                     RSAS"
     gene            complement(9452..>10077)
                     /locus_tag="PKN91_08660"
     CDS             complement(9452..>10077)
                     /locus_tag="PKN91_08660"
                     /inference="COORDINATES: protein motif:HMM:NF016974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HNS28183.1"
                     /translation="AAPSLDRALFGWIGPLEEQYTAIRGELEALLPEATGRERVFDSE
                     ALERDNLRGHHGAPGWNGYYFHRHGERREENCLRCPATTRALDALPLSRVPGHGPEVL
                     FSVFTPGTHLLPHRGVTNTRVVAHLPLIVPPDCALRVADHLHAWREGEVVVFDDTYEH
                     EAWNRSAAMRVVLIFDVWNPHLTEVERLALAALVEAIGEFRSTMEAA"
BASE COUNT         1575 a         3514 c         3393 g         1595 t
ORIGIN      
        1 caccctcgcc cgtggtctcc ccggtaggcg tgaacgcgag gttcaagtgg ccgtcccgcg
       61 gctgctcgcg atacgaggtg agcagcggct catcgtccgg cggcctcggc aggcgccgct
      121 tgcccacgag ctcgaccgcg acctcgtcgg gggcgagccc ggcgagctgc acggccaccc
      181 tcatctgcag gcgaccgaac aggtcgatct gcggcggcgg atcgctgaca cgcaccaggg
      241 ccacccgcgg ccaggccgcg cgcacgcgct gcttgaattc cgtgagcgcg cgcgcgccgg
      301 cgtaaccgtc cgcggcgagc gcggcaccga ggcgcgcggc cggccgatag atcccgtcgg
      361 cgtaatcgtt gacgacgcga cccatgttga acgcgggtat caccgtcgcc atcgcgtgct
      421 tgctcttgcg cacccactcg accggaaagc cgctgccgtt gcgggagtag tagagcggca
      481 gcacctcttc ctcgagcgtg tcgagcacgt tctcggcttc gagcgcgtcg cggcgtgcgg
      541 catcctgcac ggggaccggc gagaggcccc agccgttctc gccatcccag gcctcggtcc
      601 accagccgtc gaggatcgac aggttgagcg tgccgttggt ggcggccttg atgcccgacg
      661 ttccgctcgc ctcgagcggc gcgatcggat tgttgagcca cacgtcgcag ccggatacga
      721 gcgcgcggcc aagctgcatg tcgtagtcgt cgaggaacag gacgcgtccg gcgaattccg
      781 gtgtcagcat cagctgcttg atctcgcgca gcacggcctt gcccggctca tccgccggat
      841 gcgccttgcc ggcgaacagc agcaccatcg gtcgcgcgct gtcgttcacc aggcgcgcga
      901 ggcgcgcgcg gtcgcgcagc agcagcgagg cgcgcttgta ggtcgcgaag cgccgggcga
      961 aaccaatggt cagcacgtcc ggacgctccg gatcgatcca tcgcgaagtt tggcgccatt
     1021 gcgcgccgct catgcccttg gtcgcgaatt cggccttcag gcgctcgcgc accattgcga
     1081 acatgcgcga tttgacgtcc tgcgcggcga cccagaacgc cttgtccggt acgtgttcga
     1141 gcgtcgccca caggctgctg tcggacaggc gcccgagcca gtcgcgtgac aggtgctggt
     1201 cgaagaattc ggcccaggtc tggtgcagga aagtcggcac gtgcacgcca ttggtgacga
     1261 aaccgaccgg gttctcctgc gccgtcagtt cgggccagtg cttcgcgcac agcttcgcgg
     1321 attcgcctcg atgcacgcgg ctcacgccgt tcacatggcg cgtgcccgcg agcgcgagcc
     1381 aggtcatgtt gaattcatgc ggcgcatccg gcgcgcgccc cagtgccagc acgcgatcgg
     1441 cgggccaacc caggctgtcg aggtagctgg agaactgttc gcgcaccagg tcgtgcggga
     1501 agctgtcgtg gcccgcagcg acgggcgtat gcgtcgtgaa ggcacactgc gcggcgaccg
     1561 cctcgagcgc aacgtcggca ggcagcccgt tgcggacctc ctcgcgcatg cgttcgagga
     1621 tcaggaacgc ggcatggccc tcgttcaggt gccacactgc gggcgcgagg cccaacgcgc
     1681 gcagcgcacg cacgccgccg atgccgagca ccatttcctg gcggatgcgc gtcacgcgat
     1741 cgccgccgta gagccgatgc gtgatgtcgc gatcgaccgg tgcgttgtcc tcgcagttcg
     1801 tgtcgagcag gtagacgggc acgcgcccgg cctgggcaga ccacaggcgc gcgtgcaccg
     1861 ggcgacccgc gatgtcgatc gtgacacgca gccaggtgcc gtccgcgttg cgcaccggtt
     1921 cgaccggcag gtcgcgcgga tcgtggtcct ggtagaccgc gtgctgcacg ccgtcgctgt
     1981 cgacggtctg cacgaagtaa ccctggccgt acatcaggcc gatcgcgacg aagttcagcc
     2041 gctcatcgct cgcggccttg cagtggtcgc cggcaaggat gccgagaccg cccgagtaga
     2101 tcgggaagct ttcgtggaag ccgtattcgg cgcagaaata cgcgaccagc ggcgcatcgc
     2161 tcggcgcggc cgcctgcgcg aggtaggcat cgaaggtcgc aaggacctga ccgtaacgtg
     2221 cgacgtacac gggatccgcc gccgcgcgat ccagggcctc ctgccccacg caccgcagaa
     2281 gcagtttggc gttgccgctc gtctgcgccc agagcccggc atcgaggtcc tcgaacagcg
     2341 agcgcaccgg gcgatgccag ctgaagaaca ggttcccggc gagttccggc aatctttgca
     2401 gcttttcggg gattttagcc gttatttcta tgaagattga cgaagtcatg ggcgatttct
     2461 ggcctgggtc cctcagcggg acgattgcga tgtcggtcgg cggcgagttt agcaggactc
     2521 gcgcctacca ttgactgcgc agtattgttc acattacagt actggcaaat ggacgatagc
     2581 agcgcacgca agttccagaa ggacctcaac ggagggctgg tcgcgctcgt cctgctgtcg
     2641 gtgctcgacc aggccagggg cgagctctac ggctatgaga tcgccaagca gctgcagcgc
     2701 gccgcggacg gcgagcccct cttcaaggaa ggcaccatct acccggtgct gcggtccctg
     2761 tcggcgagcg gcctgctctc gagccgcatc gagccctcgc tgagcggacc gccgcgcaag
     2821 tattaccgca tcacggattc cggcgggatg gtgctcggtg aatggcgggc catctggcag
     2881 caaacgcgga atttcgtcga ccgcttcact tcgtaaggaa tatcgacatg aacaggacag
     2941 caccgcgctc cattgacgca tacctgaaac agctgcgcga agcgctggcg gacgccgacc
     3001 cgtcactggt gcaggacgca ctttacgatg ccgaggagta tctgcgtgcc gagctcggcg
     3061 cgcaccccgg caagtccgaa tccgacgtgc tcgaattgat cgcgagcacc tacggcgcgc
     3121 ccgacgaagt cgccgcggct taccgcgaca cggaggtcaa ggtggccgcc gccctgcaca
     3181 cgccccgccc cgccccgggc ggcggcgcct ggcgacgctt cttcagtgtg tattcagacc
     3241 cgcgcgccta tgcttcgctc ttctaccttt tcctggcgct cgcccttggc atcttccact
     3301 tcactttcac ggtggtcggc ctgtcgctgt cggccggcct cgcgatcctg atcatcggcg
     3361 tgccgttctt cctcgccttc atcggcctca cacgggtcat cgcgctcgcc gagggccgcc
     3421 tgctcgaggc ggtgtcgggc gagcgcatgc cgcgacgccc cctgcacccc ggagcgaagc
     3481 tttcgttctg gacccgcatc ctcgagatgc tgcgcgatgc gcgcacctgg accacgatcg
     3541 cctacctcat cctcgcgctg ccgctcggca tcatctactt cacgatcgcg gtgaccggca
     3601 tggcgctcgg cctgtcgctg accttcgcgc cagtcttcga gatcgggcgt cgcctcggct
     3661 ggttcgacgt cgccgccgac atccatttca gtcccgcctg gctcgcctcg ccctggctgc
     3721 tgccgctcgc gatcgcgctc ggcgtcgtga tcatcacgct gctgatgcac gccgcgcgct
     3781 tcatcggccg agtgcacgcg cgcttcgcga agcacatgct ggtgatcggc tgatgcgcgt
     3841 gaagtggccc gcacctgcgc tcgcgctcgt gatggccgcc tgctcggcgt cggacgtccc
     3901 cgatgccgga cccgcggccg agcaggccgc ggccgccacg gcgggctgcc tcgccagcgg
     3961 cgacggctac ctgcgcgccc gcattcgcgg cgcgctcgac ctcgacatcg actggcgcaa
     4021 tggcgacatc gagtgcaccg gtggtccgcg ccccgacggc agtggcctgc gcgtcacgtt
     4081 tgcgggaccc gagcagagcg acgggcgccg gctgcgcttc gtcttcggca tcgcgcaggc
     4141 ggccgagggc atcgccgcac agggcctgcc ggtgaacctc acggtgatct tcgaaggcga
     4201 gcgacgcctg ttcgcgacgc agggcgacga caagtgcacg gtcgatgcgc tcacccagca
     4261 gcgcaccggc ccgctcggcg gcgggtcgcg cagctggcgg atcgaggcgc gcggcttctg
     4321 caccgaaccg gcgtccacgc tgcagggcga cgagcgattg ctcgtgacgc gcttcgattt
     4381 cgcggggaag atcgatatag agggcgagag cccgttcaca ggtacggaaa gccatgcaag
     4441 ctgaaattcg ccggtcgcgc tctgcagcgc gcgccccgtt gctcgccgca gcggcactcc
     4501 tcgccgtgct cgcgaatgcg gacaccggac tcgaggatct cgacgccttt ccgcgcgcga
     4561 gtgtcgagat cacttcggga gagcgggtgc atcgtttcga tgtgtgggtc gccgacacgc
     4621 ccgagcgcca ggcacagggc ctgatgtacg tgcgcgatct cgatgcgggc tccggcatgc
     4681 tgttcgtgca cgcggcgccg cgcgaggtat cgatgtggat gaagaacact tacatcccgc
     4741 tcgacatgct gttcatcgat cgcggcggcc gtatcgtgcg catcgcgcac gagaccacgc
     4801 ctcactcgct cgattcgatc cggtcgggca caccggtcac gcgagtgctc gaaattcgcg
     4861 gcggcgaagc ggcgagacgc ggaatagcgg tcggggaccg tgtccgcacc aggtcgtagt
     4921 tttgatacaa caaatcgacc cgagagtggc attccgtaaa cagtctggct agaattttgc
     4981 accggttgac gcaaccccac aggctcaagt taacgagtct catgcgcaac atccccaaag
     5041 ccacagtgag atcaacatga acaacaacgt ctcgttgacg tgcgttgcgg cggcattgct
     5101 gctcgcgtcg ctcccggcag cggccgcacc gcccgctccc agctggtaca tcgcccccga
     5161 agccatctac atgtgggccg accgcgatcg cctcgccgac gacgagatcg gtggacagat
     5221 cgcattcggt cgctcgttca gcgacaagtg ggacgcggaa ctggcataca gccgcgtgag
     5281 caccgatgcg ctgggcaact cgggcctcga catcgacagc gtcttcctca acctgcaccg
     5341 cgtgttctac cgtgactcgc gcatctcgcc gttcctgaag ctcggctacg gctacgtcga
     5401 cgccgattac ggcggcggcg cactcgccgg cgagaaggtc gagtcgtgga ctcttgccta
     5461 cggcctcggg ctgctcgcga acctgaaggt ggacaccgcg cagggcaccg ccctcgcgct
     5521 gcgcgccgag atctacggtc tgtactcgac cgagaccgcg ccgccgaacc gcgatcacct
     5581 gaacgacacc gttgcgggca tcggcctgca gtaccagtgg ggcgcgcccg tggtggtacc
     5641 ggttgcggcg ccggtcatcg gcgacagcga cggtgacggc gtcaacgacg acctcgacag
     5701 gtgccccgga acaccgagcg gcacaccggt cgatgcccgg ggttgcgagc tcgacagtga
     5761 tggtgacggc gtcgtcgatc gcctcgacaa gtgcccgggc acgcccgcgg gcgccaaggt
     5821 ggacgccaac ggctgcgagc tggatggcga cggtgacggc gtcgtcgatc gcatcgacca
     5881 gtgcccgaat acgcgcgccg gcgaaaaggt cgacgaggtc ggctgctcgt tcgcattgcg
     5941 cctcgaggtg tacttcgata cggcgtcggc cgagatcaag ccggaatcgt tccgtgacct
     6001 cgacctcgcc gtgagcctga tgcagcgcaa tcagaccgtg cgcggcacga tcgagggcca
     6061 caccgacgat cgcggcaccg aggcgtacaa cctcgaactg tcgaagcgtc gcgccgcctc
     6121 ggtgcgccag tacctgatcg acaagggcgt ggcagcgagc cgcctcgagt cggtgggtta
     6181 tggcgagagc cgtccggaag cggacaatgc aaccgctgaa ggtcgcgcgc agaaccgccg
     6241 cgtggtgctg gtccggtcgg atcgataaag cgcccgtttg ctgcgacgaa gacccggtgc
     6301 cgcgaggcgc cgggtctttt ttatgcatgg tcgccgcggc aaagtgtccg catgctcgac
     6361 ctgccgggac agccatgcat cgacaagtcg cgactgatcg gcggctgcat ccgcctgccg
     6421 ctcgccgtcg atgcggcacg gctggccgcc gaagtcgcgg cgctgccggc ctcgctgtgg
     6481 ggcacgcaag gcggtcgtgt cggcgtgcat cgcccggccg aggcgatctt cctgcgcggg
     6541 catgcgcccg ccgagggcga gctgccgatc gacgatcgcg cgccgcttgc gctgctgccc
     6601 tacgcgcgcg agatcgtgac gacactgatt gcggcccccg tgcagcgctg cctgctggca
     6661 cgcctgccgg gtggtgcggt gatcgacctg cacatcgacc aggcgcccta cttcgccaag
     6721 actattcgca tccatgttcc ggtcgtgacg cacgacgagg tgtggatgct cgccggtggg
     6781 cggcgctacc ggatggccgc cggtgaggtc tgggcgctcg acaacagcgc accgcatggc
     6841 gtgcgcaacg ggcacgcaac gctctcccgc acccacctga tcgccgactt cctgccctcg
     6901 ccgccgctgc tcgcgctgct cgcgaagggc gcgcgcggtc tcggccttgc cggatgatcc
     6961 tcacgccgcg cttcgtcttc ctgcacctgc acaagtcggg cggcagcttc gtcaacgagg
     7021 ccctgctgcg ccacgaagcc ggcgcgagcc agtacggcta ccacctgccg cgcagcgcca
     7081 tcccggcatc gcatgcgcgg ctgccggtgc tgggcctcgt gcgcaatccg tggagctact
     7141 atgtgtcgtg gtatgcgttt cagcgcgcgc gcccgcgccc gaatgcactg ttcggcgtgc
     7201 tggccgacgg cgggcgggcg gactttgccg cgacgatccg caacatgctc tccctcggcg
     7261 agcacgatgc gttactcgat cgcgtgatcg ctgcgctgcc ggcgcagtac gcgaaccatg
     7321 gactcaatct cccgggtccc gcgctcgccg cgatccgcgg cacgggcctc ggcttctact
     7381 cctggctcta ccaatacctc tacggcgcgc ccgtgggcct gcacattgcg cgcatggaag
     7441 tgctgcgcac cgagctgccg caggtcatgg ctgaggccgg cgtgccggcc ggtgcagcgc
     7501 ttggcgagtt cctgcgcacg agcgcaccgc gcaacgtctc cgcgcatggc ccggcggcgg
     7561 attactacga tgccgacctg cgagcactcg tcggacgtcg cgacgccgcg ctcgtcgagc
     7621 ggtacggata cgtcgccgac tgaaacccat cgggctcgag cctgcgcaat cgatcccaca
     7681 cggacgtgat cgagggcagc acgccgagca cttcggcctc gcgccgccgc cacttgtcgg
     7741 gcgccggcgc ggtgtgcgtg tagcgcgaca gcggcagcgc gccctgcaca cggatctcca
     7801 gcgccgcgtc gaactcgatg ccggcgaagc ggcacaggcg ctcgatggtg gcgcgtggat
     7861 cggcgaccag atcctcgtaa ctcaccgccg tccagcgcgc gtgcggcagc tgctcgaggt
     7921 cctcgagcgc gatgcgattg gcgctctccc actggaacgc ggcgatctcg gcgagcggcc
     7981 ggccacgcat cgtgcgccac ccgggtggca gcagcagcga ccacggcccg tcgaagccgt
     8041 cgaggccgtt gtaggttttc caccctcccg cttcccatgc ctcgatgatg ctcgcgaggt
     8101 tgccgtgcgg atcgcgccac aggaagatga actgcgcgtc gggaaacagc cgattgatga
     8161 atggcacgcg cagcgcgttc ctgggcgtct tctcgaggaa cctgagcggg gcgtcatcgg
     8221 cgacccggcg ccccgcggca tcgacgcgtc gctccgcgag ttccgtgatg atgcgggcgg
     8281 cgatcgcatc gctcgcatgc tccgcggtga gccggttcga ctcgactccc ggcgcaccgg
     8341 ggcgcagagc cgcgagaccc tcgatcagcc acggcgcctc gccgccgagc gtcgccacat
     8401 cgtggctcgc cgcgagggtc tcgaacagca gcgtgctgcc cgaacggggt gccgcgatga
     8461 tgaacaccgg gcgcgcgggc cggcgcgcaa tgacgcgcgc gagcgacagc gccggccgca
     8521 gcacgcgggc ttcgagaacc tggttcgacg cgacgcgatt cgtcgccatg acgagtcctg
     8581 cgccggattc ctgcgcccgc gcgcgcacca catcgagcag acgctcgaat tccgtccagc
     8641 ccggcgccga ctgcgcgtgc agggcgtaga gctgccgcca gtcgcggcac agcgcatcga
     8701 gcagggcgtg gtaccgggcg aggcgcgtgg ccgcgccggg cgaatcctcg agcgcagcga
     8761 gctccgcacg caggtcgagc accagcagct cgagttcgcc cggcgacatg acacagggct
     8821 ccggtgcctg ctccgtcagc ggcatcgcat cgcgaccggg atcgaactgc agcatcggcg
     8881 cgcccggatc ctgcgaaccg gtctgccgcg tcgcgctctg ttgcacgaac tgccagaacg
     8941 cggcgctgcc ggaggtatcg gcgaccaggt ggatgcgcgg ctgcgtgccg ccatgctcga
     9001 cgcgatgcag gcgccaggtg tcgaagatcc acgcctcgcc ggtggccatg tgcacggact
     9061 cgccaccgca gtggaagcgc acctcgggct cggtcacgat cggcacgtgc agccgcacgc
     9121 ggctgaacca gtgatgattg atgtcggcat gctcgggcac gctcgcgccc ggcgcgaggc
     9181 gcagcagccg cgagcgactc cacaccaccc cgaaactcgc gagcacctgg cgcaggtagg
     9241 gcatgcgcgc gagctgcggc gtcgccagca tgcgcccgga caccgcatca ttctcgccgc
     9301 catcgaccga gatgaggcgc agcgcgctgt tgccgggaat gccgtccgga tggctcaccc
     9361 aggcatcggc cggcagcgcc tcgacctcct cgcgcagtcg ctgcgcgtcg aaccgcacgg
     9421 caaggcgctg gaagggctgc gggagacgca tctacgcggc ctccatcgtg ctgcgaaact
     9481 cgccgatggc ctcgaccagc gcggcgagcg ccaggcgctc gacctcggtc aggtgcggat
     9541 tccacacatc gaaaatcagc accacacgca tggctgcgct ccggttccac gcctcgtgtt
     9601 cgtaggtatc gtcgaacacg acgacttcgc cctcgcgcca tgcgtgcagg tggtcggcga
     9661 cgcgcagcgc gcagtccggc ggcacgatca ggggcaggtg cgcgacgacg cgcgtgttgg
     9721 tgacgccgcg atggggcagc agatgcgtgc cgggcgtgaa caccgagaac agcacttccg
     9781 gtccgtgccc gggtacgcgc gacagcggca acgcatccag ggcgcgcgtc gtggccggac
     9841 agcgcaggca gttctcctcg cgccgctcac cgtggcgatg aaagtagtag ccgttccagc
     9901 ccggcgcccc atgatggccg cgcaggttgt cgcgttcgag cgcctccgaa tcgaacacgc
     9961 gctcgcggcc cgtggcctct ggcagcagcg cctcgagttc cccgcgtatg gcggtgtact
    10021 gctcctcgag gggcccgatc cacccgaaca gggcacgatc gaggctcggt gcggccg
//
LOCUS       DAOQVY010000085        10004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_7551_length_10004_cov_6.077897, whole genome shotgun sequence.
ACCESSION   DAOQVY010000085 DAOQVY010000000
VERSION     DAOQVY010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10004
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7551_length_10004_cov_6.077897"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PKN91_08665"
     CDS             complement(<1..540)
                     /locus_tag="PKN91_08665"
                     /inference="COORDINATES: protein motif:HMM:NF015019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNS28184.1"
                     /translation="MDATMWLWLAVGAGVIAVLYGVLSTVSVLGLPAGNARMQEIAAA
                     IQAGAKAYLNRQYTTIGVVGVVLFVVLGLALGWPTAGGFAVGAILSGATGYIGMNISV
                     RANVRTAEAANNGLNAALSVAFRGGAITGMLVVGLALLGVAGYYALLAPAIGQEGALH
                     ALVGLAFGGSLISIFARLGG"
     gene            complement(633..1058)
                     /locus_tag="PKN91_08670"
     CDS             complement(633..1058)
                     /locus_tag="PKN91_08670"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HNS28185.1"
                     /translation="MHALSPVAMLMPMVAMVALTFIVWLRLYVVRVGELRERRIDPQR
                     LASAKAAGELLARTQASDNLRNLFEMPVLFYALCLAVVSVGVTSPFLVNGAWAYVVSR
                     AVHSLIHCTYNRVMHRFIVYATSCVLLAVLWAAFLARVL"
     gene            complement(1078..2331)
                     /locus_tag="PKN91_08675"
     CDS             complement(1078..2331)
                     /locus_tag="PKN91_08675"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNS28186.1"
                     /translation="MNALYAQSGGVSAVINASAAGVIDACLKHRSRIGKIYAGRDGIV
                     GALTEDMIDVTKESRATLRGLRHTPAGAFGSARFKLKGIEQNRAEYERLIEVFRAHDI
                     GYFFYNGGNDSMDTALKLSQIGSQMGYPLVCVGVPKTVDNDLPLTDCCPGFGSVAKYV
                     AVSTREAALDVASMARTSTKVFVLEVMGRHAGWIAAAGGLAGRGAGEAPHVILFPEIP
                     FEQQRFLERVRQSVTDYGYCVIVVSEGAHYADGRFLADAGTKDAFGHTQLGGAAPLVA
                     NMVREHLGYKYHWAVADYLQRAARHIASKVDVAQAYAVGKAAVELALEGRNAVMPAII
                     RKSSKPYKWTIKAVPLGKVANVEKKVPRHFITADGFGITAACRRYLEPLIAGEDYPPY
                     RNGLPDYVRIKGVPVKKRLSTSYKI"
     gene            2441..3115
                     /locus_tag="PKN91_08680"
     CDS             2441..3115
                     /locus_tag="PKN91_08680"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNS28187.1"
                     /translation="MRIVLLGAPGSGKGTQSQRLVERHGIPQVSTGDLLRAAVKAGTP
                     LGAHAKAIMDAGKLVDDATMLGIIRERLAQPDAQQGFILDGFPRTLAQAEGLEQLLQT
                     LGTPLDAVVQMEVDYDKLVKRISGRRSCTNCGRVFNIHTAPPPSAGCCAAPHLVQRPD
                     DKEETVAKRLKVYDEQTRPLVEHYAAQGLLRMIDADAGMEEVTHRLEAALAPRTGDGV
                     EVVEEA"
     gene            complement(3131..4234)
                     /gene="rnd"
                     /locus_tag="PKN91_08685"
     CDS             complement(3131..4234)
                     /gene="rnd"
                     /locus_tag="PKN91_08685"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HNS28188.1"
                     /translation="MPNSAPLIAAAGELAELGAELAAAPAIGLDTEFMRERTYRAELC
                     LLQAATPRRAVCIDPLGGAPLAALAPALGPDGPLKILHAARQDLEVLWAAIGRVGPVF
                     DTQVAAALAGFPAQVGYAELVRQLLGQELAKAHTRTDWARRPLSPEQIAYALDDVRYL
                     LALRDALLERLDKLGRLHWLEEELRALADPATFTVEPDAAWQRLKGVLSLDPGRQRLA
                     RSLAAWRERRAMDSNRPRGWILDDAVLKEIVALVPRSLAALAAIPGLPAGVAQRSGAA
                     ILELVRAAAIPDPPPPQPTRGRPDPEVTAMVKRLAELTRAAASDTGISAEVIAPRREL
                     ERLAGGARDVAVISGWRRQVVGQELLGALGESA"
     gene            4315..4839
                     /locus_tag="PKN91_08690"
     CDS             4315..4839
                     /locus_tag="PKN91_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28189.1"
                     /translation="MLSLVRLYADIALLRRGPEDLPASLFLLVATVVANFLVNFAVSL
                     VLPPIAGPWHMHLIVDIAFTLAWCAALLRVFGRPERFLQTTTAVFGYQTVLAPLWIAT
                     AWLAGRFQDDELWLVPVAILGLGMLAWMLAANGRILRSALEQPMPVCIGLVLLQTFVG
                     QLVLLGLFPSPAPP"
     gene            4839..6227
                     /gene="mpl"
                     /locus_tag="PKN91_08695"
     CDS             4839..6227
                     /gene="mpl"
                     /locus_tag="PKN91_08695"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HNS28190.1"
                     /translation="MHIHILGICGTFMAGIAAIAKSAGHRVTGSDRNVYPPMSTQLAA
                     LGIDIVQGFDAAQLDPPPDVVVIGNVMTRGVPVIEAVLDRGIPYQSGPEWLARAVLEG
                     RWVLAVSGTHGKTTTSSMLAWILEYAGLSPGFLIGGLPGNFDVSARLGAAPFFVIEAD
                     EYDTAFFDKRAKFVHYRPRTLVLNNLEYDHADIYPDLASIQRQFHHLVRIVPPSGLIV
                     ANAADPNLDATLAMGCWTPREGFARAGGAGAHWSARSSGADDYARFDVLAGGEPVATV
                     EWTQFGAHNVDNALAAIAAARHAGVPVAVAAAALGKFKGIRRRMELRGEVAGVRVYDD
                     FAHHPTAIATTIDGLRRRAGAARLVAVLEPRSNTMKLGVHRDTLAGSLEGADEVWVYA
                     PADIGWDVGGAMASLGARLHVRGDVDVLAADLVRELRAGDQLLIMSNGGFGGLHDKVL
                     AGLAQSRERVRA"
     gene            6230..6811
                     /locus_tag="PKN91_08700"
     CDS             6230..6811
                     /locus_tag="PKN91_08700"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNS28191.1"
                     /translation="MGTSTELPLFPLGTVLFPGGPLPLRIFETRYVDMVRRSLREQRG
                     FGVLWIRSGREAGEVAQTASVGTSARIVDFYQLPDGLLGLTCLGERKFRLLRRWQASD
                     GLNLGEVEWLAPEPSVPVPESLQHLALLLRAVLPQLGNLYEAVPRHFGDASWVSARIA
                     EILPIELAEKQACLELDDGVQRLARLAPLILRE"
     gene            complement(6769..7659)
                     /locus_tag="PKN91_08705"
     CDS             complement(6769..7659)
                     /locus_tag="PKN91_08705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNS28192.1"
                     /translation="MKALLWFLGLIVAGLAAIALFAWPAWLLAGPVLDVPFHRVANRV
                     GMLALLIGFIIVARRLGLADRRSLGYGLPRRAFLTEGALAVVLGIASMLPVIALLFAL
                     GLREPKESVPGTEVFGYVLGGLGSGLVVAFIEETFLRGAMYTGIARQSGARMAIIATA
                     LIYAATHFIARHRIPAAEVDAWSGVALLGGALRSFAQPLGILDAFLCLTGVGVLLGIV
                     RRLTGNIAACIGLHAGWVCVISVVRETSQRADAHALGALVSGYDGVIGWLVLAWIPVI
                     GWALVRFYSRRISGASRASR"
     gene            7756..8157
                     /locus_tag="PKN91_08710"
     CDS             7756..8157
                     /locus_tag="PKN91_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28193.1"
                     /translation="MITPSPAEVLSALAKGFDPSSGELLDATGIWQQPAITDALRAGA
                     AALGEEAASAPATAQPRPTAPANVGQPWTPQEEQELLASFAAGTPLPEIAARHLRSLT
                     AIEARLERLGKITPEQRVTRNRFTATRAPAE"
     gene            complement(8129..9013)
                     /locus_tag="PKN91_08715"
     CDS             complement(8129..9013)
                     /locus_tag="PKN91_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNS28194.1"
                     /translation="MIRWPAAAACLVAMAAGAAANADGAGPVWAIQGPNGGTVYLAGS
                     VHVLDPAQSRLPPGFDTAYRDAEQLVMEIDFDDLDELAAGEYLAANGFLADGETLRTL
                     LGPARFERLDREARKLGLPLDSVATLEPWAVAMGLLPMMFVKLDLDPALGVERQLVAR
                     ALADGKPIGGLETIEEQLAVLDGLSLEDQATFLEQTLDELDAAPTEVRRMIDAWRRAD
                     TGALAHLLLDEYGKAPALYGALVTARNQRWIPQIVALLARPDDTLVVVGALHLVGEDG
                     LLELLERRGITPRALGSR"
     gene            complement(9010..9759)
                     /locus_tag="PKN91_08720"
     CDS             complement(9010..9759)
                     /locus_tag="PKN91_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNS28195.1"
                     /translation="MIPRLIQFTDPHLYGDAAGSLRGIATLPALERAIAHARRHHWDL
                     DALLLTGDLVQDDAGGYAHIRRLFADLGKPVHCIAGNHDLIDEMRVALATTPFHIDGH
                     FDVGMWRIVMLDSSVPGSAVGHLAPPTLARLDHALTAASGRHVLVCLHHHPIDMRSAW
                     LDRVGLDNRDEFFEILDRHRNVRAVLWGHVHQQLDTTRSGVRLLATPATCAQFLPGSE
                     QFAIDRKPPAYRVLALLDDGRIDTHIEWLDE"
BASE COUNT         1573 a         3476 c         3490 g         1465 t
ORIGIN      
        1 gccgccgagg cgcgcgaaga tcgagatcag cgagccaccg aaggcgaggc cgacgagcgc
       61 atgcagcgcg ccctcctggc cgatcgccgg ggccagcagc gcgtagtagc ccgcgacacc
      121 gagcagcgcg aggcccacga ccagcatgcc ggtgatcgcg ccgccgcgaa acgcgacgga
      181 gagcgcggcg ttgaggccgt tgttggcggc ctccgcggtg cgcacgttgg cccgcaccga
      241 aatgttcatg ccgatgtagc cggtggcgcc cgagaggatg gcgccgacag cgaagccgcc
      301 ggccgtcggc cagccgagcg cgagcccgag cacgacgaac agcacgacgc cgacgacgcc
      361 gatcgtcgtg tactggcggt tgaggtaggc cttggcgccg gcctggatgg ccgcggcgat
      421 ctcctgcatg cgggcattgc ccgcgggaag gccgagtacg gaaacggtcg ataacacacc
      481 gtacaggacg gcaatcacgc ccgcgccgac ggcgagccag agccacattg tggcatccat
      541 caggagacct ccgaaaaaat tatcaaatca acgtgttatg gatgttgtcg cgaccggcac
      601 gaggcacggc gctttccctg aaaggccgcg aactatagca cacgggcgag aaatgccgcc
      661 cacagcacgg cgagcagcac gcaactcgtt gcatagacga tgaagcggtg catgacccgg
      721 ttgtaggtgc agtggatcag cgagtgcacg gcgcgcgaca cgacataagc ccaggcgccg
      781 ttcaccagga aaggggaggt gacacccacc gacaccacgg caaggcacag cgcgtagaac
      841 agcaccggca tctcgaacag gttgcgcagg ttgtcggagg cctgcgtgcg ggccagcagt
      901 tcgccggccg ccttcgcgct cgcgagacgc tgcgggtcga tgcgccgctc gcgcagctcg
      961 ccgacgcgca ccacatagag gcgcagccag acgatgaagg tgagggcgac catcgccacc
     1021 atgggcatca gcatcgcgac gggtgacagc gcgtgcatgg ccggcgtgcc gcagacgcta
     1081 gatcttgtag ctcgtcgaga ggcgcttctt caccggcacg cccttgatgc gcacgtagtc
     1141 cggcagtccg ttcctgtacg gcgggtagtc ctcgccggcg atcagcggtt cgagatagcg
     1201 gcggcaggcc gcggtgatgc cgaagccgtc ggccgtgatg aaatggcgcg gcactttctt
     1261 ttcgacgttc gcgaccttcc cgagcggcac ggccttgatg gtccatttgt agggtttcga
     1321 cgacttgcgg atgatggccg gcatcaccgc gttgcgcccc tcgagcgcca gctcgaccgc
     1381 ggccttgccg acggcatagg cctgggccac atccaccttc gaggcgatgt ggcgtgcggc
     1441 gcgctgcagg tagtcggcga cggcccagtg gtacttgtag ccgaggtgct cgcgcaccat
     1501 gttcgcgacc agcggggccg cgccgccgag ctgcgtgtgg ccgaacgcgt ccttggtgcc
     1561 ggcgtcggcg aggaagcgcc cgtcggcgta gtgcgcgccc tccgacacca cgatcacgca
     1621 ataaccgtaa tcggtgacgc tctggcgcac gcgctcgagg aagcgctgct gctcgaacgg
     1681 aatctccggg aacaggatca cgtgcggcgc ctcgcccgcg ccgcggcccg cgaggccgcc
     1741 ggccgcggcg atccagccgg catgccggcc catcacctcg agcacgaata ccttggtcga
     1801 ggtgcgcgcc atcgaggcga cgtcgagtgc cgcctcgcgc gtcgagacgg cgacgtactt
     1861 ggccaccgaa ccgaagccgg ggcagcagtc ggtgagcggc aggtcgttgt cgacggtctt
     1921 cggcacgccc acgcacacga gcgggtagcc catctggctg ccgatctgcg acagcttgag
     1981 cgccgtgtcc atcgagtcgt tgccgccgtt atagaagaaa tagccgatgt cgtgggccct
     2041 gaagacctcg atcaggcgct cgtactcggc gcgattctgc tcgatgcctt tcagcttgaa
     2101 gcgcgccgaa ccgaaggcgc cggcaggcgt gtggcgcagg ccgcgcaacg tggcacggct
     2161 ttccttcgtc acgtcgatca tgtcctcggt cagcgcgccg acgatgccgt cgcgcccggc
     2221 gtagatcttg ccgatccgcg accggtgctt caggcaggcg tcgatgacgc cggcggcgga
     2281 ggcattgatg accgcggaaa ccccgcccga ctgtgcgtat agcgcgttca ttttggtggt
     2341 ttttttcata ttgcggcaag gatagctcat ttgaccgcgg gtgcatcgcc cggtagtgtg
     2401 cgcggcttcc acgggggacc ccttcgagta tcaggaaagc atgcgcatcg tccttctcgg
     2461 cgcgcccggt tcaggcaagg gcacgcaatc tcagcgactg gttgagcgac acggcatacc
     2521 gcaggtttcc accggcgacc tgctgcgtgc cgcagtcaag gccggcacgc cgctcggcgc
     2581 gcacgccaag gcgatcatgg acgcgggcaa gctggtcgac gacgcgacga tgctcggcat
     2641 cattcgcgag cgcctggcgc agcccgacgc gcagcaggga ttcatcctcg atggcttccc
     2701 gcgcacgctc gcgcaggccg agggcctcga gcagctgctg cagacgctcg gcacgccgct
     2761 cgatgcggtc gtgcagatgg aggtcgacta cgacaagctc gtcaagcgca tctccgggcg
     2821 ccgcagctgc acgaactgcg ggcgcgtgtt caacatccac accgcgccgc cgccgtccgc
     2881 gggctgctgc gccgcgccgc atctcgtgca gcgccccgac gacaaggaag agacggtggc
     2941 gaagcgcctc aaggtctacg acgagcagac gcggccgctg gtcgagcact acgccgcgca
     3001 gggcctgctg cgcatgatcg atgccgacgc gggcatggag gaagtcaccc atcggctcga
     3061 ggctgcgctc gcgccgcgca ccggcgacgg cgtcgaggtg gtcgaggaag cctagcgcca
     3121 ggccggcagc tcaggccgat tcgccgagcg caccgagcaa ttcctgtccc accacctggc
     3181 gccgccatcc ggagatcacg gccacatcgc gggcgccgcc cgcgagtctt tcgagttcgc
     3241 gccgcggggc gatcacttcc gcgctgatgc cggtgtcgct cgcagccgcg cgcgtcaatt
     3301 ccgcgaggcg cttcaccatg gcggtgactt cggggtcggg acgcccgcgc gtcggctggg
     3361 gcggcggtgg atcgggtatc gccgctgccc gcacgagttc gaggatcgca gctccggagc
     3421 gctgcgccac gccggccggc agcccgggga tcgcggcgag cgcggcaagg ctgcgcggca
     3481 cgagcgccac gatctccttg agcaccgcat cgtcgagtat ccagccgcgc ggccggttgc
     3541 tgtccatggc ccgccgctcg cgccaggccg cgagcgacct ggcgagccgc tggcggcccg
     3601 gatcgagcga cagcacgccc ttcaggcgct gccaggccgc gtccggttcg accgtgaagg
     3661 tcgccggatc ggccagtgcg cgcagttcct cctcgagcca gtgcaggcgg ccgagcttgt
     3721 cgagccgctc gagcagtgca tcgcgcagcg ccagcaggta gcgcacgtca tcgagcgcgt
     3781 aggcgatctg ctcggggctc agggggcggc gcgcccagtc ggtgcgcgtg tgcgccttgg
     3841 cgagttcctg cccgagcagc tggcgcacga gttccgcata gccgacctgt gcagggaagc
     3901 cggcgagcgc ggccgcgacc tgcgtgtcga ataccggccc gacgcggccg atggccgccc
     3961 agagcacctc gaggtcctgg cgcgcggcat gcaggatctt cagcggccca tcggggccga
     4021 gcgccggcgc cagcgcggcg agcggggcgc cgccgagcgg gtcgatgcat accgcacgcc
     4081 gcggcgtcgc ggcctgcagc aggcacagct cggcgcgata ggtgcgctcg cgcatgaatt
     4141 cggtgtcgag gccgatcgcc ggcgcggccg ccagttccgc gccgagctcc gcgagttccc
     4201 cggcggcggc aatgagtggt gcgctgttcg gcatgcagaa tggtcccgga gactcccgta
     4261 caatcggtcc ggcattatgc gagccgaatc acgagcccga aagcaccggc cggcatgttg
     4321 agcctggtcc ggctgtacgc cgacattgcg ttgctgcgcc gtgggccgga ggatctgccc
     4381 gcgtcgctgt tcctgctcgt cgcgaccgta gtcgcgaact tcctcgtcaa tttcgccgtg
     4441 agcctcgtgc tgccgccgat cgccggtccg tggcacatgc acctgatcgt cgacatcgcc
     4501 ttcacactcg cgtggtgcgc ggcactgctg cgggtgttcg gcaggcccga gcgcttcctg
     4561 cagacgacca ccgccgtgtt cggctaccag acggtgctcg cgccgctgtg gatcgcgacc
     4621 gcctggcttg ccggccgatt ccaggacgac gaactgtggc tcgtgcccgt cgcgattctg
     4681 gggctcggca tgctggcgtg gatgctggcc gcgaacggcc gtatcctgcg ctcggcgctc
     4741 gagcagccga tgccggtgtg catcggcctc gtgctgctgc agaccttcgt cggccagctc
     4801 gtgctgctcg gcctctttcc atcgccggcg ccaccctgat gcacattcac atcctcggaa
     4861 tctgcggcac cttcatggcc gggatcgccg cgatcgcgaa atcggccgga catcgcgtga
     4921 cgggctccga ccggaatgtc tatccgccga tgagcaccca gctcgcggcg ctcggcatcg
     4981 acatcgtgca gggcttcgac gccgcgcagc tcgatccgcc acccgacgtc gtcgtcatcg
     5041 gcaacgtgat gacgcgcggc gtgccggtca tcgaggccgt gctcgatcgc ggcatcccct
     5101 accagtccgg tcccgaatgg ctcgcgcgcg cggtgctcga gggtcgctgg gtgctcgccg
     5161 tctccggcac gcacggcaag accacgacct cgagcatgct cgcgtggata ctcgaatatg
     5221 cgggcctctc gccgggcttc ctgatcggcg gcctgcccgg caatttcgac gtctcggcgc
     5281 ggctcggcgc ggcgccgttc ttcgtgatcg aagccgacga gtacgacacg gcgtttttcg
     5341 acaagcgcgc gaagttcgtg cactaccggc cgcgcacgct ggtgctgaac aacctcgagt
     5401 acgaccacgc cgacatctat ccggacctcg cgtcgatcca gcggcagttc caccacctcg
     5461 tgcgtatcgt gccgccgtct gggctgatcg tcgccaacgc cgccgatccg aacctcgacg
     5521 cgacgctggc catgggttgc tggaccccgc gcgagggctt cgcccgcgcc ggcggggcgg
     5581 gtgcgcactg gagcgcgcga tcgtcgggcg cggacgatta cgcgcgcttc gacgtgctcg
     5641 cgggcggtga gccggtcgcg acggtcgagt ggacgcagtt cggcgcgcac aacgtcgaca
     5701 atgcgctcgc ggcgatcgcc gccgcgcgcc acgccggcgt gccggtggcg gtcgcggctg
     5761 ccgccctcgg caagttcaag ggcatcaggc ggcgcatgga gttgcgtggc gaggtggccg
     5821 gcgtacgcgt gtacgacgat ttcgcgcatc accccaccgc catcgcgacg accatcgacg
     5881 gcctgcgccg gcgtgccggt gcggcgcgcc tcgtcgcggt gctcgagccg cgctcgaaca
     5941 ccatgaagct cggggtgcat cgcgatacgc tcgccggctc gctcgaaggc gccgacgagg
     6001 tgtgggtgta cgcgcctgcc gacatcggct gggacgtcgg gggcgcgatg gcgtcgctgg
     6061 gcgcgcggct gcacgtgcgc ggtgacgtcg acgtgctcgc agccgatctc gtgcgcgaac
     6121 tgcgagccgg cgatcagctg ctgatcatga gcaacggcgg cttcggcggg ctgcacgaca
     6181 aggtgctcgc tggtcttgcg cagtcgcggg agcgcgtgcg cgcctgacca tgggcacgag
     6241 cacggagctg ccgctgttcc cgctcggtac cgtgctgttt cccggcggtc cgctgccgct
     6301 gcggatcttc gagacgcgtt acgtcgacat ggtgcgccgc agcctgcgcg agcagcgcgg
     6361 attcggcgtg ctgtggatca gatcgggcag ggaagcgggc gaggtcgcgc agaccgcgag
     6421 cgtcggcacg agcgcgcgca tcgtcgattt ctaccagctg cccgatggcc tgctcggcct
     6481 cacctgtctc ggcgagcgca agttccggct gctccgccgc tggcaggcga gcgacgggct
     6541 caatctcggc gaggtcgaat ggctcgcgcc cgagccgtcg gtgcccgtgc ccgagtcgct
     6601 gcagcacctc gccctgctgc tgcgcgccgt gctgccgcag ctcggcaacc tgtacgaggc
     6661 cgtgccgcgc cacttcggcg acgcctcgtg ggtcagcgcg cgcatcgcgg aaatcctgcc
     6721 gatcgaactc gccgagaagc aggcctgtct cgaactcgac gacggggttc agcggctcgc
     6781 gcggctcgcg ccgctgatcc tgcgcgaata gaagcgcacg agcgcccagc cgatcaccgg
     6841 aatccacgcg agcaccagcc agccgatcac gccgtcgtag cccgacacga gcgcgccgag
     6901 cgcgtgggcg tccgcgcgct gcgaggtttc cctcaccacg ctgatgacgc acacccagcc
     6961 ggcgtgcagg ccgatgcagg cggcgatgtt gcccgtaagg cggcgcacga tgccgagcag
     7021 cacgccgacc ccggtgaggc acaggaaggc atcgaggatg ccgagcggct gtgcgaacga
     7081 gcgcagcgcg ccgccgagca gcgccacgcc gctccatgca tccacctcgg ccgccggaat
     7141 gcggtggcgg gcgatgaaat gggtcgcggc gtagatgagc gccgtggcga tgatcgccat
     7201 gcgcgcgccc gattgccgcg cgatgcccgt gtacatcgcg ccgcgcagga aggtctcctc
     7261 gatgaacgcg acgacgagcc cgctgccgag cccgcccagt acataaccga aaacctcggt
     7321 gccgggcacc gattctttcg gttcccgcag gccgagggcg aacagcaggg cgatcaccgg
     7381 cagcatcgag gcgatgccga gcacgacggc gagcgcgcct tcggtgagga acgcacgccg
     7441 cggcaggccg tagccgaggc tcctgcggtc cgcgaggccg aggcgccgcg cgacgatgat
     7501 gaaaccgatc agcagcgcga gcatgccgac gcggttggcg acgcgatgga acggcacgtc
     7561 gagcacgggt cccgcgagca gccacgccgg ccacgcgaac agcgcgatcg ccgcgaggcc
     7621 cgccacgatc aatcccagga accacagaag cgccttcatg cacccaattc tgcacaaaca
     7681 cacgcctttg tgcgctcgcc cgccgcggcc ggcgccgcga cactctgcgg cacatacagc
     7741 agggagggcc tggccatgat cacaccgtcc ccggccgaag tgctgagcgc gctcgccaag
     7801 ggcttcgatc catcctccgg cgaactgctc gatgccaccg gcatctggca gcagcccgcg
     7861 atcaccgatg ccttgcgcgc gggggcagcg gcgctcgggg aggaggctgc aagcgcgcct
     7921 gccaccgcgc agccccgtcc aacggcgccg gccaatgtcg gccaaccctg gaccccgcag
     7981 gaagagcagg aactgctggc gagcttcgcc gcaggcacgc cgctgccgga gatcgccgcg
     8041 cggcacctgc gctcgctcac cgcgatcgag gcacggctcg agcggctcgg caagatcact
     8101 cccgagcagc gcgtgacccg caaccggttt accgcgaccc gagcgcccgc ggagtgatgc
     8161 cccgccgctc gagcagctcg agcaggccat cctcgccgac caggtgcaac gcacccacca
     8221 ccacgagcgt gtcgtccggg cgcgcgagca acgcgacgat ctgcgggatc cagcgctggt
     8281 tgcgggccgt gacgagcgca ccgtagagcg ccggcgcctt gccgtactcg tcgagcagca
     8341 ggtgcgcgag cgccccggtg tcggcgcgcc gccaggcgtc gatcatgcgg cggacctcgg
     8401 tcggcgccgc atcgagctcg tccagggtct gctcgagaaa ggtcgcctgg tcctcgaggg
     8461 acagaccgtc gagcaccgca agctgctcct cgatcgtctc gagtccgccg atcggcttgc
     8521 cgtcggccag cgcgcgcgcg acgagctgcc gttccacgcc gagggcggga tcgaggtcca
     8581 gcttcacgaa catcatgggc agcaatccca tggcgaccgc ccacggctcg agcgtcgcca
     8641 cgctgtcgag cggcaggccg agcttgcgcg cctcacgatc gagccgctcg aaccgcgccg
     8701 gtcccagtag cgtgcgcagg gtttcgccat cggccaggaa gccgttcgcg gcgaggtact
     8761 cacccgccgc gagttcgtcg aggtcgtcga aatcgatctc catcacgagc tgctcggcat
     8821 cgcgataggc ggtgtcgaag ccggggggca gccggctctg cgccgggtcc agcacgtgta
     8881 ccgacccggc caggtacacg gtgccgccgt tcggcccctg gatcgcccag accggccccg
     8941 cgccgtccgc attcgcggcg gcgccggccg ccatcgcgac caggcaggct gccgcggcgg
     9001 gccagcggat cattcgtcga gccattcgat gtgcgtgtcg atccgcccgt cgtccagcaa
     9061 ggcgaggaca cgataggcgg gcggcttgcg gtcgatcgcg aactgctcgg agcccggcag
     9121 gaactgggcg caggtcgccg gggtcgcgag cagccgcacg ccgctgcgcg tcgtgtcgag
     9181 ttgctggtgc acatgtcccc acaggaccgc gcgcacgttg cgatggcgat cgaggatctc
     9241 gaagaactcg tcgcgattgt cgagcccgac ccggtcgagc caggcgctgc gcatgtcgat
     9301 cgggtggtgg tgcaggcaga cgagcacgtg ccgcccgctc gccgcagtaa gcgcgtgatc
     9361 gagccgcgcg agcgtgggcg gcgcgaggtg cccgacggcg ctgcccggca ccgagctgtc
     9421 gagcatcacg atgcgccaca tgccgacatc gaaatgcccg tcgatgtgga acggcgtcgt
     9481 cgcgagcgcg acgcgcatct cgtcgatcag gtcgtggttg cccgcgatgc aatgcaccgg
     9541 tttgccgagg tccgcgaaca gccggcggat atgcgcgtag ccgcccgcat cgtcctgtac
     9601 caggtcgccg gtcagcagca gcgcatcgag atcccaatgg tgccggcgcg cgtgcgcgat
     9661 ggcgcgctcg agcgcgggca gggtggcgat gccgcgcagg ctgccggctg catcgccgta
     9721 gagatgcgga tccgtgaact ggatgagtcg cggtatcacg ggcagaattt tagcaaaggg
     9781 gtcgctgcga caaggtgatg cattaccatc gcagtgcgtc gtgattcggc gacggcgcgc
     9841 cacgtcacgc cgcgcgtgcg tggtattgtg aatcagacca cggcagggcc gcgatatgac
     9901 caattcgacg atcgccatca cgagccagtt gcagcgctac ctcatcgagc acaccgtgcg
     9961 cgagccggca atcctcgctg cgctgcgcca gctcaccggg aaga
//
LOCUS       DAOQVY010000086        10001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_7555_length_10001_cov_4.421375, whole genome shotgun sequence.
ACCESSION   DAOQVY010000086 DAOQVY010000000
VERSION     DAOQVY010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10001
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7555_length_10001_cov_4.421375"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PKN91_08725"
     CDS             complement(<1..670)
                     /locus_tag="PKN91_08725"
                     /inference="COORDINATES: protein motif:HMM:NF015272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HNS28196.1"
                     /translation="MSSEGAPGCPFAPSERVDLSDEHVHVDLAVSQSYGGYLALDKLL
                     DAQHPNSREHDELLFITVHQTSELWIKLMLHELAGVLACVRRDDLDPSFKMFARISRI
                     QGQLLAVWDVLSTMTPADYSAFRNSLGRASGFQSVQYRLLEFQLGNKNADYLATFRRD
                     PQAHARLERALEAPSLYDEALRLLSRRGYGVPDDYVVRDFRQPYRASKQVAGAWLGVY
                     HNAEK"
     gene            complement(660..1409)
                     /locus_tag="PKN91_08730"
     CDS             complement(660..1409)
                     /locus_tag="PKN91_08730"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNS28197.1"
                     /translation="MPETATSQLSLFDSREVASAWTLRVSRRARRLTVRVFHTGRVEV
                     VVPPRTSAQAVRDFLQRHRQWVESRRERARRELPPSAPFPPQTVTLAAFGEHWTVQAV
                     GGNGRGRLLPTGEGRLEIHGVLSGETLRHQLRTWVVAQATTRLRPWLVSLAREGGFSF
                     GRMSVRRQRTRWGSCSSRGTISLNASLAFQRPAVVRYLLLHELAHTRHMNHSSRFWRL
                     VATHEPDWRELDRELLGGWRRVPDWMFNDVE"
     gene            1465..2349
                     /locus_tag="PKN91_08735"
     CDS             1465..2349
                     /locus_tag="PKN91_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS28198.1"
                     /translation="MSPLLEVRNLTKLYPRVTAVDGVSFSVEEGACFGLLGPNGAGKT
                     TTIEIIEGILPPSAGEVLYRGEPLGRRFRAEAGILFQKTALQDFLTVRQTLTLFRGLY
                     TQGYDIDELLELCSLQKLADRDHRKLSGGQQQRLLLAVALVNDPAILFLDEPTTGFDP
                     QARRNFWRLIESVKARRRTIILTTHYMDEAQRLCDEIAIMDRGRIIAQGAPRELVSSH
                     FADVLIELPRADFTPAARALPLRVIEGTERVEITTDDIDQTLKALMAVHVPLRNLRIR
                     PPNLEDLFLELTGKELRP"
     gene            2346..3356
                     /locus_tag="PKN91_08740"
     CDS             2346..3356
                     /locus_tag="PKN91_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS28199.1"
                     /translation="MIRRLLAVWHARNLEFLRDRGSLIFTLLLPLTLVLGMSFVFGGP
                     ERALFKVAVLTDDLGSIEHPFMIERYVDFIASDDAAAAVRKVARHQIDLLLDTAADGS
                     RYWVNPDSPKGYIVERMLLQADPAAERQPVSGDAVRYVDWLLPGILGMNIMFSCLFGV
                     GYVVLRYRKNGFLKRLHATPITAFEFLAAQVLSRLGLILVVTVILFVGTSYLIGLRSV
                     GNPALLLLIATLGSLSLIALGLTIAARFSSDELVGGLLNTLTWPMMLLSGVWFSLEGS
                     PEWVRAIAKVFPLTHMLDAARAVMLDGAGLAQVAPQLGYLAITTLVFLAFGAWSFRWR
                     TD"
     gene            3370..4413
                     /locus_tag="PKN91_08745"
     CDS             3370..4413
                     /locus_tag="PKN91_08745"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HNS28200.1"
                     /translation="MRFYSDNNATVCPELLAAIHEANSAPTRGYGDDRWTQALDEAFG
                     RFFGTAVKVFPVTTGTAANALALATTVPPYGAVYAHDEAHVVRDECGAPEFFSGGLRL
                     VSLHGGGAMLDAATLERALDAMPPSVHTVQPAAVTITQATELGRVYQPAAIAALAALA
                     HARGLHVHMDGARIANALATLGCSPAAVTWQAGVDVLSFGATKNGGLAAEAVVFFRPD
                     LVRDFELRRKRAGHLLSKSRYVSAQLLAYVSTDVWRRNAERANSAARRIGAACTYGLL
                     EPVEANEVFVRIGEPAKATLRARGFEFYDWGAAQSGEARFVASWDQDPREVDALCRAL
                     AELQNSGGAISPP"
     gene            complement(4400..5119)
                     /locus_tag="PKN91_08750"
     CDS             complement(4400..5119)
                     /locus_tag="PKN91_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28201.1"
                     /translation="MAMIRRAAKLLLCATFAPAAALAADWADLEGRIQFAYFTEDVRS
                     LRSTLASPANRTSTDALTDYYLGLGEYRVLLLSIDRDRDAAKRAAEACVDHLDAANGA
                     QRDFADGLALQAACQSLLATLKPWKAPLLTPRSNTLFARAHMLAPRNPRVLLLEALAD
                     KDAGTAHGKLQAAVEAFEAERQRVAPIPSWGAAEAYAHLGRIDLERGDAVGARSELER
                     ALLLAPDFALARRLLATITAD"
     gene            5141..5977
                     /locus_tag="PKN91_08755"
     CDS             5141..5977
                     /locus_tag="PKN91_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNS28202.1"
                     /translation="MNQDNTAPPALIDIGANLTHDSFDMDRDAVIARARAVGVVQQIV
                     TGASVAGSAAAIGLARAHAGELFATAGVHPHHAAELDEPAFGRLAQLAAAPEVVAVGE
                     CGLDYFRDYSPRDAQRRAFARQLELAALVRKPVFLHQRDAHGDFTAILREHRDVLVGG
                     VAHCFTAGADELGDYLDLGLAIGMTGWICDERRGQHLVPLMARIPAGRLMLETDAPYL
                     LPRDLAPKPASRRNEPMYLPHVAAAVARAREESVAALAAHTTRAARQLFGLPEAIRSG
                     GV"
     gene            complement(5958..6557)
                     /locus_tag="PKN91_08760"
     CDS             complement(5958..6557)
                     /locus_tag="PKN91_08760"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HNS28203.1"
                     /translation="MRDLPATPVLVGLVLSGVALGLLGSWGWRSWRTRRARRRLVNAI
                     TSTGFEHLQDVLVPDGNGSTYHIDFLVLTPRGILVVDLRDVSGNIFGGDQMTDWTVMN
                     GAMRHTFPNPQGPLYDRVAAVKALSGQVPVEGRVVFTRRARFPKGLPKWTMPVDTLRA
                     EFPPADRGMFQEVLVQFREGWEAVRSGSAPSTLVKPRPI"
     gene            complement(6554..7879)
                     /gene="thrC"
                     /locus_tag="PKN91_08765"
     CDS             complement(6554..7879)
                     /gene="thrC"
                     /locus_tag="PKN91_08765"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNS28204.1"
                     /translation="MLYISTRGGSPAVTFSEALLAGLAPDGGLYVPATWPQFAVEDFA
                     GCTSLAEVARTLIAPFAAGDPLADQLATITAEACDIPAPLVPVGHGAGYSVLELFHGP
                     TAAFKDFGAQFLAACLTRLRPPGARPLEILVATSGDTGGAVAAAFHGRAGIRVGVLFP
                     HGLVSPLQQQQLTCWGDNVQAFAVRGSFDDCQRLVKAAFVDPQLRERFDLSSANSINL
                     GRLLPQAAYHAATALAVWREQGVKPSFVIPSGNLGNALACIWARHVGLPIGEIVLAHN
                     ANRTVPDYLDGKPWQPRRSIATLASAMDVGDPSNMERLLALFPALPDLQRAVSAHVVD
                     DGAIRHRISADHAAYGMIWCPHTATAAEVMARMPAERKARGPWIIVATAHPAKFSEIV
                     APLIGRPVPVPDSLARLYQRPASSRPLEPQLDALRAALIGDDAGPGSTS"
     gene            complement(7885..8820)
                     /locus_tag="PKN91_08770"
     CDS             complement(7885..8820)
                     /locus_tag="PKN91_08770"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNS28205.1"
                     /translation="MTRNEATAFAPASVGNVAIGFDILGFSVDALGDRVTVRRSARPG
                     VTITAITGVVENLPLEATGNTAGRALLALREAVSPDTGYEMVIHKGIPLGSGLGGSAA
                     SAVGAVVAANALLDAPLPPIDLLRFAMQGEAVASGSMHVDNIAPSLFGGLVLTVGIDQ
                     PRVKQIPVPAAVRAIIVHPHMVLGTREARAMLSKNVKMSDFVWQTANLAGFISGCYTD
                     DLDLIRASFEDVIIEPQRQSLIPGFREVRESAMSAGALGCSISGAGPTMFAWSTADSA
                     EAVRSAMVESFAARGTQTDHWVTGIEPRGARIVAD"
     gene            complement(8817..>10001)
                     /locus_tag="PKN91_08775"
     CDS             complement(8817..>10001)
                     /locus_tag="PKN91_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28206.1"
                     /translation="VRAIAQGASERNISVVVNGKDTTRALRAVHAGFYLSPHTLSIGV
                     IGPGTVGRVLLDQLAAQEQRLRGLQLDLRVRGILASRRMALADGSIALPRWREALEAS
                     TTPADLARFVENIDVDHLPHAVIIDCSASAEVAAHYAAWLEAGIHIVTPNKKANSGEL
                     AYYRKLQAVRRASGAHYLYETTVGAGLPVIQTLRDLRDTGDELRSIEGIFSGTLAYLF
                     NVYDGSVPFSRIVADAKQRGYTEPDPRDDLSGTDVARKLIILGREIGLDLELRDVQVE
                     SLVPAGLEQGTIEAFLQRLSQSDAAMQERYQAARARGKVLRYVGRITAAGEASVGVVE
                     VDANHAFANIALTDNIVRFATARYSANPLIVQGPGAGPEVTAGGVFADLLRLAAYLGA
                     RL"
BASE COUNT         1557 a         3442 c         3440 g         1562 t
ORIGIN      
        1 ccttctcggc attgtgatag acaccgagcc acgcgccggc cacctgcttg ctcgcgcggt
       61 atggttgcct gaagtcgcgc accacgtagt cgtccggtac accatagccg cggcgtgaca
      121 gcagccgcag cgcctcgtcg tacagcgagg gtgcctcgag ggcgcgctcg agccgcgcat
      181 gggcctgcgg gtcgcgacgg aaggtggcca ggtagtccgc gttcttgttg ccgagctgga
      241 actcgagcag ccggtactgg accgactgga agcccgatgc gcgcccgagc gagttgcgaa
      301 acgccgagta gtcggccggg gtcatggtcg agagcacgtc ccagacggcc agcagctgcc
      361 cctggatgcg cgaaatacgc gcgaacatct tgaatgacgg gtcgaggtcg tccctgcgca
      421 cgcaggcaag cacgccggcg agttcgtgca gcatcagctt gatccacaat tcggaagtct
      481 ggtgcacggt gatgaacagc agctcgtcgt gctcgcgtga attggggtgc tgcgcatcga
      541 gcagcttgtc gagcgccagg tagccgccgt aggactgcga gacggcgagg tccacgtgca
      601 cgtgttcgtc ggagagatcg acacgctcgc tcggcgcgaa cgggcaacca ggcgcgcctt
      661 cactcgacat cgttgaacat ccagtcgggc acacgtcgcc agccaccgag cagctcgcga
      721 tcgagctcgc gccagtccgg ctcgtgcgtg gcgacgaggc gccagaagcg ggaggaatgg
      781 ttcatgtggc gggtgtgggc gagctcgtgc agcagcaggt agcggacgac ggccgggcgc
      841 tggaaggcca ggctggcgtt caggctgatc gtgccacgac tcgagcagct gccccaacgg
      901 gtgcgctgac ggcgtaccga catccggcca aagctgaagc cgccctctct cgccagcgat
      961 acgagccagg gccgcagcct ggtcgtggcc tgtgccacca cccaggtgcg cagctggtgg
     1021 cgcagtgtct ccccggacag caccccgtgg atctccagcc ggccctcgcc cgtcggcagc
     1081 aggcggccgc ggccgttgcc cccgacggcc tgtacggtcc agtgctcgcc gaacgcggca
     1141 agcgtgacgg tctgcggcgg gaacggtgcc gacggcggca attcgcgccg cgctcgctcg
     1201 cgccgcgact ccacccactg gcgatggcgc tgcaggaagt cgcgcacggc ctgcgcggac
     1261 gtgcgcggcg gcaccacgac ctcgacgcga ccggtgtgga agacccgcac cgtcaggcgg
     1321 cgcgcacggc ggctgacgcg cagcgtccac gcggaggcaa cctcgcgcga atcgaacagc
     1381 gacagctggg aagtggcagt ttcgggcatg gtgtcctggg gaccggcgct gccatgatat
     1441 cagccgatcg gctagcatga acgagtgtcg ccactcctcg aagtccgcaa tctcaccaaa
     1501 ctctatccgc gggtgaccgc cgtcgatggc gtgtcgttca gcgtcgaaga gggcgcgtgc
     1561 ttcggcctgc tcggcccgaa cggtgcgggc aagaccacca ccatcgaaat catcgaaggc
     1621 atcctgccgc cgtcggcggg cgaagtgctg tatcgcggcg agccgctcgg ccggcgtttc
     1681 cgtgccgaag caggcatcct gttccagaag accgcattgc aggacttcct gaccgtccgc
     1741 cagacgctca ccctcttccg cgggctctat acgcagggct acgacatcga cgaactgctc
     1801 gagctgtgct cgctgcagaa actggccgat cgcgaccacc gcaaactctc cggcggccag
     1861 cagcagcgcc tgctgcttgc ggtggcgctg gtcaacgatc cggcgatcct gttcctcgac
     1921 gagccgacca cgggtttcga cccgcaggcg cggcgcaatt tctggcggct gatcgaatcg
     1981 gtgaaggcgc ggcggcgcac gatcattctc acgacgcact acatggacga ggcgcagcgg
     2041 ctctgcgacg agatcgcaat catggatcgc ggccgcatca tcgcccaagg tgcgccgcgc
     2101 gagctcgtgt ccagccattt cgccgacgtg ctgatcgagt tgccgcgggc cgatttcacg
     2161 cccgctgcgc gcgcgttgcc gttgcgcgtg atcgagggca ccgagcgggt cgagatcacg
     2221 accgatgaca tcgaccagac gctcaaggca ctgatggcgg tccatgtgcc gctgcgcaac
     2281 ctgcgcatac gcccgcccaa cctcgaggat ctgttcctcg agctgaccgg caaggaactg
     2341 cggccgtgat ccggcgcctg ctcgcggtct ggcatgcgcg caacctcgag ttcctgcgcg
     2401 atcgcggctc gctcatcttc acgctgctgc tgccgctcac gctcgtgctc ggcatgagct
     2461 ttgtattcgg cgggcccgag cgcgccctgt tcaaggtcgc ggtgctgacg gatgacctcg
     2521 gcagtatcga gcatccgttc atgatcgagc gctacgtcga tttcatagcg agcgacgatg
     2581 ccgcggcggc ggtgcgcaag gtcgcgcggc accagatcga cctgctgctc gacaccgcag
     2641 cggacggctc ccgctactgg gtcaatccgg attctcccaa gggctatatc gtcgagcgca
     2701 tgctgctgca ggccgatccg gctgccgagc gccagcccgt gagtggcgac gccgtgcgct
     2761 acgtggattg gctgctgccc ggaatcctcg gcatgaacat catgttcagc tgtctgttcg
     2821 gtgtcggcta cgtggtactg cgctaccgca agaacggctt cctgaagcgc ctgcacgcga
     2881 cgccgatcac ggccttcgag tttctcgccg cccaggtgct gtcgcgcctc ggactgatcc
     2941 tcgtcgtgac ggtcatcctg ttcgtcggca cgagctacct gatcggcctg cgttcggttg
     3001 gcaacccggc attgctgctg ctgatcgcga cgctcggctc tctgtcgctg atcgcgctgg
     3061 gcctgacgat cgcggcgcgc ttctcgagcg acgaactggt cggcggcctg ctcaacaccc
     3121 tcacctggcc gatgatgctg ctctccggag tgtggttttc gctcgagggc tcgccggaat
     3181 gggtgcgcgc catcgccaag gtctttccgt tgacgcacat gctcgatgcc gcgcgggcgg
     3241 tgatgctcga tggtgcgggc ctcgcgcagg tggcgccgca gctcggctat ctcgcgatca
     3301 ccacgctcgt attcctcgcc ttcggcgcgt ggtcgttccg ctggcgcacc gactgaccgg
     3361 ggtcgccgca tgcgtttcta cagcgacaac aacgcgacgg tctgtcccga actgctggcc
     3421 gcgatccacg aggccaattc cgcaccgacc cgcggctacg gggatgatcg ctggacccag
     3481 gcgctggatg aggccttcgg gcgcttcttc ggtaccgccg tcaaggtgtt cccggtcacg
     3541 accggcaccg cggcgaatgc gctggcactc gccacgaccg tgccccccta tggtgccgtt
     3601 tacgcgcacg acgaggcgca cgtggtgcgc gatgaatgcg gcgcgccgga gttcttcagt
     3661 ggtggcctgc ggctcgtgtc actgcatggc gggggcgcca tgctcgatgc cgcgacgctc
     3721 gagcgcgcgc tcgacgccat gccgccgtcg gtgcacacgg tgcagccagc cgccgtgacg
     3781 atcacgcagg cgaccgagct ggggcgcgtc taccagcccg cggccatcgc cgcgctcgcg
     3841 gccctggcac atgcgcgcgg actgcacgtg cacatggatg gtgcgcgcat cgccaatgcg
     3901 ctcgcgacgc tgggctgcag ccccgccgcg gtgacctggc aggccggcgt cgacgtgctg
     3961 tccttcggcg cgaccaagaa cggcggcctg gctgccgagg cggtcgtgtt cttccgcccc
     4021 gacctcgtgc gcgatttcga gttgcggcgc aagcgtgccg ggcacctgct gtccaagtcc
     4081 cggtatgtct cggcgcaatt gctggcctat gtgtcgacgg atgtctggcg ccgcaacgcc
     4141 gagcgcgcca actccgccgc gcgccggatc ggcgcggcct gcacatacgg cctgctcgag
     4201 ccggtcgagg ccaacgaggt cttcgtgcgc attggcgagc ccgccaaggc cacgctgcgc
     4261 gcgcggggat tcgagttcta tgactggggt gccgcgcagt cgggcgaggc gcgtttcgtc
     4321 gcctcctggg atcaggatcc ccgcgaggtc gatgccctgt gccgcgcgct cgcggaactg
     4381 cagaattccg ggggcgcgat cagtccgccg taatcgtcgc gagcagccgg cgcgccagcg
     4441 cgaagtccgg cgcaagcagc aacgcccgct cgagctcact gcgcgcgccc acggcatccc
     4501 cgcgctcgag atcgatgcgg ccgaggtgtg cataggcttc ggcagcgccc cacgacggga
     4561 tgggcgcgac acgctggcgt tcggcctcga acgcctcgac cgcggcctga agtttgccgt
     4621 gggcagtgcc ggcatccttg tcggcgagcg cctcgagcag aagcacgcgc ggattgcgcg
     4681 gcgcaagcat gtgggcacgc gcgaacagcg tattgctgcg cggtgtcagc agcggtgcct
     4741 tccagggctt cagcgtggcg agcagggact ggcaggcggc ctgcagggcc aggccgtcgg
     4801 caaagtcgcg ctgcgcgcca ttggccgcat cgaggtgatc gacacaggcc tcggccgcgc
     4861 gcttggcggc atccctgtcg cggtcgatgc tgagcagcag cacacgatac tcgccgaggc
     4921 ccaggtagta atcggtgagc gcatcggtgc tcgttcgatt cgcaggcgac gcgagcgtgc
     4981 tgcgcagcga gcgcacatcc tcggtgaagt acgcgaactg gatgcgcccc tcgagatcgg
     5041 cccagtccgc ggcgagggcg gcggccggcg cgaacgtggc gcacaggagc agcttggcag
     5101 cgcgtcggat catggccatg cagttagaat tcgtcgaatc gtgaaccaag ataacacagc
     5161 gccccctgcc ctgatcgaca tcggggcgaa cctgacacac gacagtttcg acatggaccg
     5221 cgatgcggtg atcgcgcgcg cacgtgccgt cggcgtcgtg cagcagatcg tgaccggcgc
     5281 gagcgtcgca ggcagcgccg ccgccatcgg acttgcgcgt gcgcatgccg gcgagctgtt
     5341 cgcgaccgcc ggcgtgcatc cccatcacgc cgcggaactc gacgagccgg ccttcgggcg
     5401 cctcgcgcag ctggccgcgg cacctgaggt cgtggccgtc ggcgaatgcg gcctcgatta
     5461 cttccgcgat tactcaccgc gcgatgccca gcgccgcgcc ttcgcgcgcc agctcgagct
     5521 cgccgcgctg gtgcgcaagc cggtgttcct gcaccagcgc gacgcgcatg gcgacttcac
     5581 ggcgatcctg cgtgagcacc gcgacgtgct ggtcggtggc gtcgcgcact gcttcaccgc
     5641 cggggccgat gagctcggcg actacctcga tttgggcctc gcgatcggca tgaccggctg
     5701 gatctgcgat gagcgccgcg gccagcatct cgtgccgctc atggcgcgga tacccgccgg
     5761 gaggctgatg ctcgagacgg acgcgcccta ccttctgccg cgcgacctcg cgccaaaacc
     5821 cgcgtcacgc cgcaatgagc ccatgtacct gccgcacgtc gcggcagccg tggcgcgggc
     5881 gcgggaggaa tccgtcgccg cgctcgctgc gcacacgacg cgcgccgcgc gacagctgtt
     5941 cggcctgccc gaggcgatca gatcgggcgg ggtttgacca gcgtgctcgg cgcactgccc
     6001 gatcgcaccg cttcccagcc ctcgcggaac tgcaccagca cttcctggaa catgccgcga
     6061 tcggccggtg ggaattcggc gcgcagggtg tccaccggca tcgtccactt gggcagcccc
     6121 ttggggaagc gcgcgcgacg cgtgaacacc acccgcccct cgacgggcac ctggccgctc
     6181 aatgccttga ccgcggcgac acgatcgtag agcggccctt gcggattcgg gaacgtgtgg
     6241 cgcatcgcgc cgttcattac cgtccagtcg gtcatctggt cgccaccgaa gatattgccg
     6301 ctcacgtcgc gcaggtcgac gaccaggatg ccgcgcggcg tcagcaccag gaagtcgatg
     6361 tgataggtgc tgccgttgcc gtcgggtacc agcacgtcct gcaggtgctc gaatccggtc
     6421 gaggtgatgg cattgacgag tcgccggcgc gcacgccgcg tccgccacga gcgccagccc
     6481 cacgagccga gcaagccgag cgcaacaccg ctcagaacga ggccgacgag cacgggcgtc
     6541 gcgggcaggt ccctcatgac gtgctccccg ggccggcatc gtcgccgatg agtgccgcgc
     6601 gcagcgcatc gagctgcggc tcgagcggcc ggctcgacgc aggacgctgg tagaggcgcg
     6661 cgagggaatc gggcacgggc accggacggc cgatcaatgg cgcgacgatt tcgctgaact
     6721 tcgccgggtg tgctgtcgcg acgatgatcc acgggccgcg cgccttgcgc tccgccggca
     6781 tgcgcgccat cacctcggcg gcggtcgccg tgtgcgggca ccagatcatg ccgtaggcgg
     6841 cgtggtccgc actgatgcga tggcgaatcg cgccgtcgtc gaccacgtgc gccgacaccg
     6901 ctcgctgcag gtcgggcagc gcagggaaca gtgccagcag cctttccatg ttgctcggat
     6961 cgccaacgtc catcgcggag gccagcgtag cgatactgcg ccggggctgc cagggtttgc
     7021 cgtccagata gtcgggtacc gtgcgattgg cgttgtgggc gagcacgatc tcgccgatcg
     7081 gcaggccgac gtggcgcgcc cagatgcagg ccagggcatt gccgaggttg ccgctcggaa
     7141 tgacgaacga tggcttcacg ccctgctcgc gccatacggc gagcgcggtc gccgcgtggt
     7201 aggcggcctg cggcagcagg cggccgagat tgatgctgtt ggccgatgac agatcgaacc
     7261 gttcgcgcag ttgcgggtcg acgaaggccg ccttgacgag ccgctggcag tcatcgaagc
     7321 tgccgcgcac ggcgaaggcc tgcacgttgt cgccccagca ggtgagctgc tgctgctgca
     7381 gcggcgaaac gagcccgtgc gggaacagca cgccgacgcg tatgcccgcc cgcccgtgga
     7441 atgccgcggc cacggcgccg ccggtgtcgc cggaggtggc gacgagtatc tcgagcgggc
     7501 gtgcgccggg cgggcgcagg cgggtgaggc aggccgccag gaactgcgcg ccgaagtcct
     7561 tgaatgccgc cgtaggccca tgaaacagct cgaggaccga atagcccgcg ccgtggccca
     7621 ccggcacgag gggcgccgga atgtcgcagg cctcggcggt aatggtggcg agctgatcag
     7681 ccagcgggtc gcccgccgca aacggcgcga tcagcgtgcg cgcaacctcc gcgagcgagg
     7741 tgcagcccgc gaagtcttcg acggcaaact gcggccacgt ggccggtacg tagaggcctc
     7801 cgtcgggagc caggcccgcg agcaacgctt ccgagaaggt gactgcgggg ctgccgcccc
     7861 gcgtgctgat gtacaacatg tcgctcaatc cgccacgatc cgtgcgccgc ggggctcgat
     7921 cccggtgacc caatggtcgg tctgcgtgcc gcgtgccgcg aaactttcca ccatggccga
     7981 gcgcaccgct tcggccgaat cggccgtgct ccaggcaaac atggtaggtc cggcgccgga
     8041 gatcgaacag ccgagcgccc cggcggacat cgccgactcg cgcacctccc ggaaacccgg
     8101 gatcagcgac tgccgctgtg gctcgatgat cacgtcctcg aacgatgcac gtatcaggtc
     8161 gagatcgtcc gtgtagcaac ccgatatgaa gccggccagg ttcgcggtct gccacacgaa
     8221 gtccgacatc ttgacgttct tgctgagcat cgcccgcgcc tcgcgcgtgc cgaggaccat
     8281 atgcggatgg acgatgatgg cacgcacggc cgcgggcacc gggatctgct tgacccgcgg
     8341 ctggtcgata ccgaccgtga gcacgaggcc accgaacagc gacggcgcga tgttgtcgac
     8401 gtgcatcgat ccgctcgcga ccgcctcgcc ctgcatcgcg aaccgcagca ggtcgatggg
     8461 cggcagcggc gcatcgagca gcgcattggc cgccaccact gcacccaccg ccgaggccgc
     8521 cgagccgccg aggcccgaac cgagcgggat acctttgtgg atgaccatct cgtagcccgt
     8581 atcgggcgag accgcctccc gcagcgcgag cagcgcccgg cccgcggtgt tgcccgtggc
     8641 ctcgagcggc aggttctcca ccacgccggt gatggccgtg atcgtcaccc cagggcgcgc
     8701 actgcgcctc acggtgacac gatcgccgag cgcatcgacg ctgaagccga ggatgtcgaa
     8761 gccgatcgcc acgttgccga ccgatgccgg cgcgaaagcc gtcgcctcat tgcgcgtcac
     8821 agccgcgctc ccaggtaggc cgcgaggcgc agcaggtcgg cgaagacgcc gcccgccgtc
     8881 acctcgggtc cggcgcccgg accctgcacg atcagcggat tggcgctgta tcgcgcggtc
     8941 gcaaagcgca cgatgttgtc ggtgagcgcg atgttggcga aggcatggtt cgcatccacc
     9001 tcgacgacgc cgacgctcgc ttcgcccgcc gcggtgatgc gtccgacata gcgcaacacc
     9061 ttgccgcgcg cccgcgccgc ctggtagcgc tcctgcatgg cggcatccga ctgcgacagg
     9121 cgttgcagga acgcctcgat cgtgccctgc tcgagcccgg ccggcacgag actctcgacc
     9181 tgcacgtcac gcagttcgag gtcgaggccg atctcgcggc cgaggatgat cagtttgcgc
     9241 gcgacatcgg tgcccgacag gtcgtcgcgc ggatccggtt cggtgtagcc gcgctgcttc
     9301 gcgtcggcga cgatgcgcga aaacggcacg gagccatcgt acacattgaa caggtaggcg
     9361 agcgtgccgg agaagatgcc ctcgatgctg cgcagctcgt cgccggtgtc gcgcagatcg
     9421 cgcagcgtct ggatcaccgg cagcccggcg ccgacggtgg tctcgtagag atagtgcgcg
     9481 ccgctcgcgc gccgaaccgc ctgcagcttg cggtagtacg ccagctcgcc gctgttcgcc
     9541 ttcttgttgg gtgtgacgat atggatgccc gcctcgagcc acgcggcata gtgcgccgcc
     9601 acttcggcgc tcgcgctgca atcgatgatc accgcgtgcg gcaggtggtc gacgtcgatg
     9661 ttctcgacga agcgcgcgag atcggcgggc gtggtgctcg cctctagcgc ctcgcgccag
     9721 cgaggcagcg caatgctgcc atcggccagc gccatcctgc gcgaggcgag gatgccgcgc
     9781 acgcgcaggt cgagctgcaa gccgcgcagg cgttgttcct gggcagcgag ctggtcgagc
     9841 agcacgcgcc ccaccgttcc ggggccgatc accccgatcg agagcgtgtg cggcgagaga
     9901 tagaacccgg catgcacggc gcgcaatgcc cgcgtcgtgt ccttgccgtt gacgaccacc
     9961 gagatgttgc gttccgacgc gccctgcgcg atggcccgca c
//
LOCUS       DAOQVY010000087         9954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_7602_length_9954_cov_4.948884, whole genome shotgun sequence.
ACCESSION   DAOQVY010000087 DAOQVY010000000
VERSION     DAOQVY010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9954
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7602_length_9954_cov_4.948884"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PKN91_08780"
     CDS             <1..753
                     /locus_tag="PKN91_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixG Ig-like domain-containing protein"
                     /protein_id="HNS28207.1"
                     /translation="CKYMCPYARFQSAMFDRDTLIITYDERRGEPRGARKKGSDHKAR
                     GLGDCVDCTMCVQVCPTGIDIRKGLQYECIACAACIDACDEVMDKVGYPRGLIRYDTQ
                     SGLEGKRRHVVRPRTIVYASLLALLVTGFAVTLSVRNEVGVDVIRDRNALYRERSDGR
                     IENVYNVKVLNKDRDAHEFRISATGVEGLEVDYVGPTVYVAPGAVQSVPVRIRVPRSA
                     LQGGTDLVVSIEAVGRPDLAASGKARFIAPTD"
     gene            766..999
                     /locus_tag="PKN91_08785"
     CDS             766..999
                     /locus_tag="PKN91_08785"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNS28208.1"
                     /translation="MIDSTPKPWYRHLWPWLLIVPPAAAVIGGFLVLYLALTRPDALV
                     SEDCFKDGVTMVCGEEARRILESAGRDGDRPAQ"
     gene            996..3473
                     /locus_tag="PKN91_08790"
     CDS             996..3473
                     /locus_tag="PKN91_08790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNS28209.1"
                     /translation="MSASGHPCFHCGEPIPRGLVIHARVAGRDEAVCCHGCKAVAEFI
                     SGAGLGDYYRYRDTASTRADEPPRPDRWAAYDRPELVERLTRAEPDGARSITVLLEGL
                     RCAACSWLANKALSQQNGVLDVSVNPATARARLVWDPARVRLGDLLRVLEHVGLRPHP
                     LAGEPSEQMALLERRDALKRLAVAGFGNMQVMMFAVPLYFGAVSGIDPQMREYMRLVS
                     MLVSVPVALYSGWPFYRGAWHALRARSISMDVPVSLGILLAFVASVWNALTGHGEVYF
                     DSVSMFVFFLGLGRYVEMIARHRAGSVADALARLAPMTARRVRAGATEDVQAVELGVG
                     DELLVRAGEVFAADGVVLEGDGRVDESMLTGESVAVPKPPGTAVHQGTQNLAAPLRVR
                     VTAVAGGTVLSGIVALLERAQAERPRLARAADRAAAWFLSRILFGAAIVYALWWFVDP
                     SRAFPATLAVLVVTCPCALSLATPTALAAATAALARRGVLVARTDAIETLAKATRVLW
                     DKTGTLTRGLVRVEDVRSLGAISSQQALEWAASLEQLSEHPIARAFTVTGAPTVRASD
                     VEVHAGRGIEGRIEGRRLRVGTRAFAAGLQSQATADAGPDAAGDESGESRVYLGDESG
                     LLAAFTLSDPLRPEAGHCVAGLAALGLPSEIVSGDQPGAVARIAARTGIDGHAARLSP
                     QDKLERLKSLQAGGAVVVAVGDGINDAPLLRGADVAIAMGRGSALAQTSADLILVRES
                     LDDLPETVSLARRTQRVIRQNLGWAIGYNLAALPLAAFGLVPPWLAAIGMSLSSVVVV
                     LNAMRLSRGSAPRRRSGMPATPQAEAA"
     gene            3475..3672
                     /gene="ccoS"
                     /locus_tag="PKN91_08795"
     CDS             3475..3672
                     /gene="ccoS"
                     /locus_tag="PKN91_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNS28210.1"
                     /translation="MEILYVMIPLGLVLVALGLWAFFWATGSGQFDDLDSPGWTVLED
                     DEAPPPDEPQPQDDAPREQGR"
     gene            3669..4409
                     /locus_tag="PKN91_08800"
     CDS             3669..4409
                     /locus_tag="PKN91_08800"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNS28211.1"
                     /translation="MMNVDPGAVSLGAALVAGFAASGHCLGMCGGIAGALAMRRPEAG
                     FGARLGYALAYNLSRITSYAVAGALAGLLGRTLLAAVNVKPLSIAFRVVAGAIMIAAA
                     VQIVSGWRLLGPLEAAGSRLWRRVAPWAGRQGRAGGLGGAVALGLAWGWLPCGMTYSM
                     LLLAATTASAVTGAAVMLAFGVGTLPSMVTAGVAFERVARGLARRATLRNVAGALLLA
                     FGLWTAGNALWHLLPSGQEGQGHEHMHH"
     gene            complement(4522..5109)
                     /locus_tag="PKN91_08805"
     CDS             complement(4522..5109)
                     /locus_tag="PKN91_08805"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNS28212.1"
                     /translation="MRFLKITLIGAVTFVLPLALIGALIAIAVQFALRLLMPIEHVLP
                     RATVLGVSAPHFTAALVVLLVCHLAGVLATTGTGQRLRDWFERHAAAKLPGYSMVRTL
                     LAGVIPSDKSVEVALVEMENMRVMAFVMERHANGCATIFVPSAPTPTVGTLFIARADQ
                     VQRVDVRLKDAMMCISRLGVGAERLLAGMKQADSG"
     gene            complement(5216..>5677)
                     /locus_tag="PKN91_08810"
     CDS             complement(5216..>5677)
                     /locus_tag="PKN91_08810"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNS28213.1"
                     /translation="YFEQRFAETVTHCHRRGEPLALMVLDLDNLKPVNDTLGHEVGDQ
                     LLVAFGRRMKSTLRESDLLARIGGDEFIVVVEDVKGEADAAAIAEKLLAALCEPLDAA
                     GHSIAASASIGIAVCRASESPQDLMKRADRALYRAKRAGRARYHIDVLEPA"
     assembly_gap    5678..5777
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5778..6868)
                     /locus_tag="PKN91_08815"
     CDS             complement(<5778..6868)
                     /locus_tag="PKN91_08815"
                     /inference="COORDINATES: protein motif:HMM:NF028467.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HNS28214.1"
                     /translation="MSRRVGMAGGPVEESQLFLATLPADRGERRLALATVLASAAVFA
                     AVAPFARQPLAEIPAFLGIYQSALVICDLITAVLLFGQFAILGSRALLVLASGYLFSA
                     AMAVLHALSFPGLFSPAGLLGAGPQTTAWLYFFWHLGFPAATIGYALLRDASTDGAAR
                     RTRAIALAVAAAATVAAALALLATAGHEALPVIMRGHADAPAKYVVAWGTLLFTLAAL
                     PALWRRRGLSVLDLWLMVVICAWAFDVALAAVFNAGRYSVGWYAGRVYGLAAASFVLV
                     VLLLENSSLYARLARSQQAAQLRRRALESMMESIADGIMMLDPDGTIRSWNAAATRMF
                     GYSAEEVVGRNIAMLLPEDQRSAQGEAMRR"
     gene            complement(7192..7653)
                     /locus_tag="PKN91_08820"
     CDS             complement(7192..7653)
                     /locus_tag="PKN91_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS28215.1"
                     /translation="MMSPVKTRPAEARDLDALAALFDAYRQFYEQPADLALARRYLGE
                     RFERRESVILVAEHGDGTIVGFAQLYPAFCSVAADRTFVLYDLFVTPAARGTGAGRTL
                     MQAAEAHARAAGAARIELQTAKTNVIGQSLYESCGWKRDELFYNYAKSLRD"
     gene            7820..8251
                     /locus_tag="PKN91_08825"
     CDS             7820..8251
                     /locus_tag="PKN91_08825"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HNS28216.1"
                     /translation="MERIARWVERGVIYVTQVLLMLVIIAALLQLWILFAIAIRDHVA
                     DFDTVPLLTEAVQRAFAGVLLIVLGLELLETLRVYFEQRRVRLEIILVVAIIAVARHV
                     INLDFHNLSGAEIAGTAALFLALTGGYYLVRKSGVDAPFGS"
     gene            complement(8248..>9954)
                     /locus_tag="PKN91_08830"
     CDS             complement(8248..>9954)
                     /locus_tag="PKN91_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNS28217.1"
                     /translation="PRDMTAVRPSRDRYVNQEERFHFDNVVVDAVRREIRVDGRVVET
                     QPKVFDLLHYLIANRDRVVDRNELMEHLWPGVIVTDASLTQALKKARRIVGDDGDRQA
                     VIRTVQRHGFRFVAALGPPAGRAAVEPATGQAPSTTGSVAVLAFVDMSPGKDQEHFCD
                     GMAEEIINSLSRVEGLRVAARTSSFAFKGSAADVREIATKLGVAAVVEGSVRREGDRL
                     RVVAQLVEASSGFHRWSERWDRPLVDVFSIQDEIATGVARALKEQFTSADRAAIPAQR
                     ARDMGAYDFYLRGLELRARFGRRSQRYALEMFRRALGIDANYAPAWAGLATSYMLLCQ
                     TGTSEEYRRLASEAAARAAALDPNSVDAQIACAATATLLGDHAAADRAFLRAEELCPR
                     SFDAWCFHGRACAAQGEYARAAELYEVAARIRPEDYEVPLLAQQCYQRLGRHEQALDA
                     TRRSAAAAERALELDPHDVRALSLGCGSLILLSRLEQAREWLQRACALEPDEPDVRYN
                     AACALAVLGEHDRALDLLEQLSAGEADAYRSWMEHDAWLDPLRGHQRFQRLFARKGGS
                     PV"
BASE COUNT         1479 a         3441 c         3460 g         1474 t
ORIGIN      
        1 tgcaagtaca tgtgcccata cgcgcgcttc cagagcgcga tgttcgaccg cgacacgttg
       61 atcatcacct acgacgagcg gcgcggcgag ccgcgcggcg cgcgcaagaa gggcagcgac
      121 cacaaggcga ggggcctcgg cgactgcgtc gactgcacca tgtgcgtgca ggtctgcccg
      181 accggcatcg acatacgcaa gggcctgcag tacgagtgca tcgcctgcgc ggcctgcatc
      241 gacgcctgcg acgaggtgat ggacaaggtc ggctacccgc gcggcctgat ccgctacgac
      301 acgcagagcg gcctcgaggg caagcgcagg cacgtcgtcc gcccgcgcac gatcgtctat
      361 gcatcgctgc tcgcactgct ggtcaccggc ttcgccgtca ccctgtcggt ccgcaacgag
      421 gtgggcgtgg acgtgatccg cgatcgcaat gcgctctacc gcgagcgctc ggacggccgc
      481 atcgagaacg tctacaacgt gaaggtcctg aacaaggacc gcgacgcgca cgagttccgc
      541 atcagcgcca cgggcgtcga gggcctcgag gtcgactacg tgggcccgac cgtgtacgtg
      601 gcgcccggcg ccgtgcagag cgtgccggtg cgcatccgcg tgccgcgcag cgcgctgcag
      661 ggcggcaccg acctggtggt gagcatcgag gcggtcggcc ggcccgacct ggccgcgagc
      721 ggcaaggccc gtttcatcgc accgacggac tgataggctg cacgcatgat cgactccacg
      781 cccaagccct ggtaccgcca cctctggccc tggctgctga tcgtgccgcc ggcggcggcc
      841 gtcatcggcg gcttcctcgt gctgtacctc gctctcacgc gccccgatgc gctggtcagc
      901 gaggactgct tcaaggacgg cgtgaccatg gtctgcggcg aggaagcccg gagaatcctc
      961 gagagcgccg ggcgcgacgg ggaccggccg gcgcaatgag cgccagcggg catccctgct
     1021 tccactgcgg ggagcccata ccacgcgggc tcgtcatcca cgcgcgcgtc gcggggcgcg
     1081 acgaagccgt ctgctgccac ggctgcaagg ccgtcgccga gttcatcagc ggcgcgggcc
     1141 tcggcgacta ctaccggtac cgcgacacgg cgagcacgcg cgccgacgaa ccaccgcggc
     1201 ccgaccgctg ggcggcctat gaccgccccg aactggtgga gcgcctgacg cgggccgagc
     1261 cggacggcgc ccgctcgatc accgtgctgc tcgagggtct gcgctgcgcg gcgtgcagct
     1321 ggctggcgaa caaggcgctc tcgcagcaga acggcgtgct cgacgtgagc gtgaaccccg
     1381 cgacggcgcg ggcgcgcctg gtgtgggacc cggccagggt gcgcctcggc gacctgctgc
     1441 gcgtgctcga gcacgtgggc ctgcgcccac acccgctcgc gggcgagccg tcggagcaga
     1501 tggccctgct cgagcggcgc gacgcgctga agcgcctcgc ggtcgcaggc ttcggcaaca
     1561 tgcaggtgat gatgttcgcc gtgccgctgt atttcggcgc ggtgtcgggc atcgacccgc
     1621 agatgcgcga gtacatgcgg ctcgtgagca tgctggtgtc ggtcccggtg gcgctgtact
     1681 ccggctggcc gttctaccgc ggtgcctggc atgcgctgcg cgcacgcagc atcagcatgg
     1741 acgtgccggt gagcctcggc atcctgctcg cgttcgtcgc gagcgtgtgg aacgccctca
     1801 cgggccacgg cgaggtgtac ttcgactcgg tcagcatgtt tgtgttcttc ctcgggctcg
     1861 gccgctacgt cgagatgatc gcgcgccacc gggccggcag cgtggccgac gcgctcgccc
     1921 ggctggcgcc gatgaccgcg cgccgcgtgc gagccggcgc caccgaggac gtgcaggccg
     1981 tcgagctcgg ggtcggcgac gagctcctgg tgcgcgcagg cgaggtgttc gcggcggacg
     2041 gcgtggtgct cgagggcgac ggccgcgtgg acgagtcgat gctgaccggc gagtcggtcg
     2101 ctgtgcccaa gccgccgggg acggcggtgc accagggcac gcagaacctc gccgcgccgc
     2161 tgcgcgtgcg cgtcacggcc gtcgcgggcg gcacggtgct ctcgggcatc gtggcgctgc
     2221 tcgagcgcgc gcaggccgag cggccccgcc tcgccagagc cgccgaccgc gccgccgcct
     2281 ggttcctcag ccgcatcctg tttggggcgg cgatcgtgta cgcgctctgg tggttcgttg
     2341 atccgtcgcg cgccttcccg gcgacgctgg cggtgctggt ggtcacctgc ccctgcgcgc
     2401 tgtcgctcgc cacgcccacg gcgctcgcgg ccgcgaccgc cgccctggcg cggcgcggcg
     2461 tgctggtcgc gcgcaccgac gccatcgaga cgctggcgaa ggcgacgcgc gtactgtggg
     2521 acaagaccgg cacgctcacc cgaggcctgg tgcgcgtcga ggacgtgcgc agcctcggcg
     2581 cgatctcgag ccagcaggcg ctcgagtggg cggcctcgct cgagcagctg tccgagcatc
     2641 cgattgcgcg ggcgttcacg gtgaccggcg caccgaccgt gcgcgcgagc gacgtcgagg
     2701 tgcacgccgg ccggggcatc gagggacgca tcgaggggcg gcggctgcgc gtcggcacgc
     2761 gcgcgttcgc cgcgggcctg cagtcgcagg cgaccgccga cgcggggccc gatgccgccg
     2821 gcgatgagtc gggcgagagc cgggtctacc tcggcgacga atcgggcctg ctcgcggcgt
     2881 tcacgctctc ggacccgctg cgacctgagg caggccactg tgtcgccggc ctcgcggcgc
     2941 tgggactgcc gtcggagatc gtgagcggcg accagccggg cgcggtcgcc cgcattgctg
     3001 cacgcaccgg catcgacggc cacgccgcgc gtctctcgcc gcaggacaag ctcgagcgcc
     3061 tgaagtcgct gcaggccgga ggcgccgtgg tagtcgcagt gggcgacggc atcaacgacg
     3121 cgcccttact gcgcggggcg gacgtggcca tcgccatggg acgcggctcg gcgctggccc
     3181 agaccagtgc cgacctgatc ctggtgcgcg aatccctcga tgaccttcca gagaccgtct
     3241 ccctcgcccg ccgcacgcag cgggtgatcc gccagaacct cggctgggcc atcggctaca
     3301 acctggcggc tctgccgctc gccgcattcg gcctcgtgcc gccgtggctc gccgcgatcg
     3361 gcatgtcgct gagttccgtg gtggtggtgc tgaatgccat gcgcctgtcg cgcggatcgg
     3421 cgccgcgacg gcgatcgggc atgcccgcga ccccgcaggc ggaggcggcc tgacgtggag
     3481 atcctctacg tcatgattcc gctcggactg gtgctggtcg cgctcggcct gtgggcattc
     3541 ttctgggcga cgggcagcgg ccagttcgac gatctcgaca gcccgggctg gacggtgctc
     3601 gaggacgacg aggcgccgcc gccggacgaa ccgcagccgc aggacgacgc tccgagggag
     3661 cagggccgat gatgaacgtg gatcccggtg ccgtgagcct cggcgccgcg ctggtggcgg
     3721 gattcgcggc gagcggccac tgcctcggca tgtgcggcgg catcgcgggc gcactcgcca
     3781 tgcgccgtcc cgaggccggc tttggcgcgc ggctcggcta cgcgctcgct tacaacctgt
     3841 cgcggatcac gagctacgcc gtcgccggcg cgctcgccgg gctgctcggc cgcaccctgc
     3901 tcgccgccgt gaacgtcaag cccctgtcga tcgcgttccg cgtcgtggcc ggcgcgatca
     3961 tgatcgcggc ggccgtgcag atcgtgtccg gctggcgact gctcggcccg ctcgaggccg
     4021 cgggatcgag gctgtggcgc cgtgtcgcgc cctgggcggg ccggcagggc cgggcgggcg
     4081 ggctcggcgg ggcggtcgcc ctcgggctcg cctggggctg gctgccctgc ggcatgacct
     4141 attcgatgct gctcctcgcc gccacgaccg ccagcgcggt gacgggcgcg gccgtcatgc
     4201 tcgcgttcgg cgtgggcaca ctgccgtcga tggtgaccgc cggcgtcgcg ttcgagcgcg
     4261 ttgcgcgcgg cctcgccagg cgcgcgacgc tgcgcaacgt cgctggtgcg ttgctgctcg
     4321 cgttcgggtt gtggacggcg gggaacgcgc tgtggcacct gctgccctcc ggccaggagg
     4381 ggcaggggca cgagcacatg catcactgac cccggcgcga tcctgttcct gcggccgcaa
     4441 tggctgatgc aaggcgccgc gatcagcgaa attcgtgctg tcgggcgacc cgacgggaac
     4501 tccccacgtg aggacgtgcc gctaaccgct atccgcctgt ttcattccgg ccagcagtct
     4561 ctccgcgccc acacccagcc gcgaaatgca catcatggcg tcctttaaac ggacgtcgac
     4621 gcgttgcact tgatctgcgc gggcaatgaa taaggtcccg actgtcggtg tcggcgcgct
     4681 cggcacgaaa atcgtggcgc agccgttcgc atgccgctcc atgacaaagg ccatgacccg
     4741 catgttttcc atttcgacga gcgcgacctc gaccgacttg tccgacggga tgacgccggc
     4801 gagcagcgtg cgcaccatgg agtagcccgg aagttttgcg gctgcatgtc gctcaaacca
     4861 gtcgcgcagt cgctggcctg ttcccgtggt ggccaacacg cctgccaggt ggcaaaccag
     4921 caggacgacg agcgcggcag tgaaatgcgg ggctgagacg cccagcacgg tcgcgcgcgg
     4981 cagtacatgc tcgatcggca tgagcaatct caatgcgaac tgcacggcta tcgcgatcaa
     5041 cgcgccgatg agcgcgagcg gcaacacgaa cgtcactgcg ccaatcagtg tgatcttgag
     5101 gaaacgcatg attgagccct catagattgg acagacggaa tccagcagag caacgcacag
     5161 aagacgcgcc ttcggaggca gaacgcgcac gcaacggcgt cacgggaatc tgacctcaag
     5221 ccggctccag aacgtcgatg tgatagcgcg cgcggccagc acgcttcgcc cggtacaggg
     5281 cccggtcggc gcgcttcatc agatcctgcg gcgattcgct tgcgcggcac accgcaatgc
     5341 cgatgcttgc ggaggcggcg atcgaatggc cggccgcgtc gaggggttcg cacagcgcgg
     5401 cgagcagttt ctcggcgatc gccgcggcgt ctgcctcgcc cttcacgtcc tcgacgacca
     5461 cgatgaactc atcgccaccg atccgtgcca gcagatcgct ttcgcgcaat gtggacttca
     5521 tccgccggcc gaacgcgacc agcagctgat cgccgacctc atgtcccagc gtgtcgttca
     5581 cgggcttgag gttgtcgagg tcgagtacca tgagcgcgag cggctcgccg cggcgatggc
     5641 agtgcgtgac cgtctcggcg aagcgctgct cgaagtannn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnncgt cgcatcgcct caccctgcgc cgagcgctga tcttcgggca
     5821 ggagcatcgc gatgttgcgc ccgacgactt cctcggccga atagccgaac atacgcgtcg
     5881 ccgcggcgtt ccaggagcgg atcgtgccgt cggggtccag catcatgatg ccgtcggcga
     5941 tgctctccat catcgactcc agcgcacggc gccgaagctg tgctgcctgc tgcgagcgcg
     6001 ccaggcgcgc gtagagacta ctgttctcga gcagcagcac gaccagcacg aagctcgcgg
     6061 cagcgaggcc gtagacgcgg cccgcgtacc agcccaccga gtagcggccg gcgttgaaca
     6121 ccgccgccag tgcgacatcg aatgcccacg cgcagatcac caccatcagc cacaggtcga
     6181 ggaccgacag cccgcggcgg cgccatagcg ccggcagcgc agcgagcgta aagagcagcg
     6241 tgccccaggc gacgacgtac ttcgccggag catcggcgtg gccgcgcatg atcaccggca
     6301 gcgcttcgtg gcccgccgtg gcgagcagcg ccagagcggc ggcgactgtc gcggcagcgg
     6361 cgaccgcgag cgcgatggcc cgcgtgcgcc gcgccgcgcc gtcagtcgat gcgtccctga
     6421 gcagggcgta gccgatcgtc gccgcgggga atccaagatg ccagaagaaa tacagccagg
     6481 cggtcgtctg cgggccagcg ccgagcagac ccgccggcga gaacaggccc ggaaacgaca
     6541 gcgcgtgaag caccgccatc gccgcgctga acaggtagcc gctcgcgagg accaatagcg
     6601 cgcgcgagcc caggatcgcg aactggccga agagcagcac cgccgtgatc aggtcacaga
     6661 tgacgagcgc cgattggtag atgccgagga aagccgggat ctcggcgagc ggctggcgcg
     6721 cgaacggcgc cacggcagcg aacaccgccg ccgaggcgag caccgtcgcc aatgccagcc
     6781 gccgctcgcc gcgatcggcc ggtagcgtcg cgaggaagag ttgcgactct tcgaccgggc
     6841 cgcccgccat gcccaccctt ctgctcatcg aatcgtgtcg gtacccccga gcgtcggcga
     6901 cggcgtgcgc cgcccaccac tgcgcgttgt accgggcctg gtcggcgcat ctcacgaggt
     6961 cccgggacga gccgccagca agtgctttta gcagatgccg cgcgcgagac ccatctggtg
     7021 ccgctggatc tgatggcagg acttgcagcg ctggtgccgc ccacgcggaa cactggccat
     7081 aaggctcgtt acgcgaacca ggccagccgg ccagagcagg ccgtttgaat ttcctgcccg
     7141 cgcggggctt cgcgacgccg gcctgtcccg ttacgcggaa ggcgccgatg actagtcgcg
     7201 caggctcttg gcgtagttgt agaacagctc gtcgcgtttc cagccgcaag actcgtacag
     7261 cgattgcccg atgacgttgg tcttcgcggt ctgcagctcg atccgcgccg ctccggccgc
     7321 gcgcgcgtgc gcctccgccg cctgcatcag cgtgcgcccg gctcccgtgc cgcgcgccgc
     7381 cggcgtgacg aagaggtcgt agagtacgaa ggtgcggtcc gctgcgaccg aacagaaggc
     7441 agggtacagc tgcgcgaagc cgacgatggt gccgtcgcca tgctcggcga ccaggatcac
     7501 cgattcgcgg cgctcgaacc gctcgccgag gtagcggcgg gcgagggcga ggtccgcggg
     7561 ctgctcgtag aactggcggt aggcgtcgaa cagcgcggcc agcgcatcga ggtcgcgcgc
     7621 ttcggcggga cgggtcttga ctggggacat catcagttct ccctacggtc cggcagcatc
     7681 ttatctagca gctcaaagcc ggggcgctcg cgcccgcctg cagcggggcc cgcccgtacg
     7741 gcgccatgat cttcgcgaag ctacgcgtcc ccttcacaaa ccgctatgct cggccgatcg
     7801 tcggatgggg ggagcgcgga tggaacgcat tgcgcgatgg gtggagcgcg gggtcatcta
     7861 cgtgacccag gttctgctga tgctggtcat cattgccgcg ctgttgcagc tctggatcct
     7921 gttcgccatc gcgatccgcg accacgtcgc cgacttcgac acggtgccgc tcctcacgga
     7981 agcggtgcag cgcgcgttcg ccggcgtgct gctgatcgtg ctcggcctcg agctgctcga
     8041 gacgctgcgg gtgtacttcg agcagcgccg cgtgaggctc gagatcattc ttgtggtggc
     8101 gatcatcgcc gtcgcccgac acgtcatcaa cctcgacttc cataacctgt ccggcgcgga
     8161 gatcgccggc accgcggcgc tgttcctcgc gctgacaggc ggctactacc tcgtgaggaa
     8221 gtcaggcgtc gacgcgccgt ttgggtctta gacgggactg ccgccctttc tcgcgaacaa
     8281 tcgctgaaat cgctggtggc cccgcagcgg gtcgagccac gcgtcgtgct ccatccatga
     8341 gcgatatgcg tccgcttccc ccgcgctcag ttgttcgagc agatcgaggg cgcggtcgtg
     8401 ctcgcccagc actgccaacg cgcaggccgc gttgtagcgg acatcgggct cgtcaggctc
     8461 gagcgcacag gcgcgctgca gccattcgcg cgcctgctcg agtcgcgaca gcaatatgag
     8521 cgacccacag ccgagcgaca gcgcgcgcac gtcgtgcggg tcgagctcca gcgcgcgctc
     8581 ggccgcggcc gcggaccgac gggtcgcatc cagcgcctgc tcgtgccgtc cgagccgctg
     8641 atagcactgc tgggccagca gcgggacctc atagtcttcc gggcgtatgc gcgcggcgac
     8701 ctcatagagc tcggcggcac gcgcatactc gccctgcgcg gcgcaggcac gcccgtgaaa
     8761 gcaccacgcg tcgaatgaac gggggcacag ttcctccgcg cgcagaaagg cgcgatcggc
     8821 cgccgcatgg tcgccgagca gggtggccgt ggcggcgcat gcgatctggg catcgaccga
     8881 gttcggatcg agtgcggccg cacgcgccgc ggcctccgag gcgaggcgcc gatactcctc
     8941 ggatgtcccg gtctggcaga gcagcatgta gctcgtggcc aggcccgccc acgccggagc
     9001 atagttcgcg tcgataccca gcgcccggcg gaacatttcg agcgcgtaac gctggctgcg
     9061 ccggccaaag cgggctcgca gctcgagtcc gcggagatag aaatcgtagg cgcccatgtc
     9121 ccgcgcgcgc tgggcgggaa tcgcggcgcg gtcggcgctc gtgaactgct ccttcagcgc
     9181 ccgggcgaca ccggtcgcga tttcgtcctg gatcgagaac acgtccacga gcggacgatc
     9241 ccagcgctcg gaccagcgat gaaagccgct cgacgcctcg acgagttgag cgacgaccct
     9301 cagacgatcg ccttcacgcc ggacactccc ttcgacgacg gccgccacgc cgagtttcgt
     9361 cgcaatctcg cgcacgtccg ccgcgctgcc cttgaaggcg aagctcgagg ttcgcgccgc
     9421 cacgcgcagt ccctccacgc ggctcagcga gttgatgatc tcctcggcca tgccgtcgca
     9481 gaagtgctcc tggtccttcc ccggactcat gtccacgaag gcgagcaccg cgacggaacc
     9541 ggtcgtggac ggtgcctggc cggtcgccgg ctccacggcc gcccgcccgg cgggcgggcc
     9601 gagcgccgcc acgaaacgaa agccgtgtcg ctgcaccgtg cggatcacgg cctggcggtc
     9661 gccgtcgtcg ccgacgatcc tgcgagcctt cttgagcgcc tgcgtcagcg aggcgtcggt
     9721 cacgatcacg cctggccaca gatgctccat gagctcgttc cggtcgacga cgcggtcacg
     9781 gttcgcgatg aggtaatgca gcaggtcgaa gaccttgggt tgtgtctcga cgacgcgccc
     9841 gtcgacgcgg atctcgcggc ggacggcatc tacgaccacg ttgtcgaagt ggaaccgttc
     9901 ttcctggttc acgtaacgat cacgggaagg tcggacggcg gtcatgtctc gcgg
//
LOCUS       DAOQVY010000088         9944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_7614_length_9944_cov_5.214885, whole genome shotgun sequence.
ACCESSION   DAOQVY010000088 DAOQVY010000000
VERSION     DAOQVY010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9944
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7614_length_9944_cov_5.214885"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2179)
                     /gene="cphA"
                     /locus_tag="PKN91_08835"
     CDS             complement(<1..2179)
                     /gene="cphA"
                     /locus_tag="PKN91_08835"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNS28218.1"
                     /translation="MRVLERSVYAGPSHYARFPVIRLELDLGELEQWPTVRLGPAFVD
                     SLVEALPGLAGHGCSYREPGGFLRRMREGDGTWLGHVLEHVAIELQNVAGEDVTFGKT
                     RSVDGRPGVYTVVYEFAQRDEGVAAGELGLRLLASLLPPQLRTAASVPEGWDWPAARD
                     DFIRFAQRRALGPSTASLVRAAEARGIPWLRLNDQSLVQLGHGKYQQRIQATVTGRTP
                     HIAVELASDKEETNRILASLGLPVPRQELVQTAEQAVRAAQRIGFPVVTKPYNGNHGR
                     GISIRLMSGAEVAAGFAAAREHSRSVIVETFLEGDDHRLLVVNGELVAATRRTPGQVV
                     GDGAHSVAELIDLVNLDPRRGVGHEKVLTRLELDAQAVRMLERAGLTAESVPAPGQVV
                     PLRSTANLSTGGTATDVTDVIHPDNRDMAVRAVRAIGLDVGGVDFLSRNIAESYRRIG
                     GGICEVNAAPGFRMHVSPSEGISRDVAGPVIDMLFPRGAPSRVPIAAITGTNGKTTTA
                     RMLAHIAKMAGFTPGLTTTDGVYIDGQRTVQGDMTGPVSARMVLADPQIDLAVLETAR
                     GGLLRAGMGVQEVNVGAVLNVQSDHLGLKGIDTLEQLAEIKRVVVEVATDCAVLNADD
                     PLTLRMSGYTDAQSICYVTMTPQHNLVREHVRAGGRACALEAGVNGQMITLYDRGGHI
                     PLLWTHLIPATLEGRATHNVQNAMFAAAMAFSLGIKLDAIRQGL"
     gene            complement(2183..3022)
                     /locus_tag="PKN91_08840"
     CDS             complement(2183..3022)
                     /locus_tag="PKN91_08840"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNS28219.1"
                     /translation="MPSKVPDGEARGWIVPIGGAENKENDKRILARFVAVSGGSDADI
                     VVIPTASRLHETGSRYEQLFRQLGAARVAVMDFDTRRDCHEQGRLDRLGEASGIFFTG
                     GNQLRLTTLLGGTPVAKLIRGRNARGVTVGGTSAGASILSEHMIAFGDDGSSAISGSV
                     RLAPGLGLTNRFVIDQHFRQRDRLGRLLTALAYNPFSVGIGLDEDTAAFIGPDDTLEV
                     EGSGGVTVVDAADVSFSSMDLVSEGQPICMLGLKLHLLVSGATFNLHTRIASAVALRA
                     LKE"
     gene            complement(3156..4397)
                     /locus_tag="PKN91_08845"
     CDS             complement(3156..4397)
                     /locus_tag="PKN91_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28220.1"
                     /translation="MRKTLQLAPLLAMALLAVGSVGISGPAVAADQKAPAVSKALAKP
                     LKAAQDALGKKDWRGALAELDKADATSGKSAYDQFLIAEMRGFAYVRLNNFAEAGRNL
                     EAGLNSGFLAQEDVASRVRALAQISYQNKNYGKAVEYGNRAIKGGFADADLYTLVGQA
                     YYVQNDFKGTHKFMDSYVGDQIRRGQTPKKQSLELILSSCVKQKDDECTTAALERLVK
                     YYPSPEYWQNIMYSLFRAPDRDDKVLFNTFRLAADVDALSRSEDYTEMAQLAIEQGSP
                     GEAVSILEKGFAKDVFADQRAKDKNQRLLDLAKKSAATDQAALAKLATETGSAKTGGA
                     DVGLGKGYLSYGEYDKAAAAIQRGLTKGGLSDASEATLLLGISQLKGGNKSDAVKTFD
                     TIKTDARYGRLASLWAIHAQS"
     gene            complement(4509..4943)
                     /locus_tag="PKN91_08850"
     CDS             complement(4509..4943)
                     /locus_tag="PKN91_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNS28221.1"
                     /translation="MSMNVGSASGEDEPMVEMNTTPLIDVMLVLLIMFIITIPVMTHA
                     VKLDMPQPQPDQPLPEVRPEVIDIEIDFDGTIVWNGTVVPSIATLEGYFRQESQKDPQ
                     PELHLRPDARSRYDTVAQVLASAQRNRMLKIGFVNTAEFREL"
     gene            complement(4957..5385)
                     /locus_tag="PKN91_08855"
     CDS             complement(4957..5385)
                     /locus_tag="PKN91_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNS28222.1"
                     /translation="MNVGTGSADDEPKAMSEINTTPLVDVMLVLLIIFLITVPVITKT
                     VPVELPKATNIATQTRPENITVAVDERGNMYWNNGQVPDTQTLLEFVKAAAVQVPQPE
                     IHIRGDRNARFEAVGRVIYTIQRGGVVKVGFITEPDRGVR"
     gene            complement(5404..6159)
                     /locus_tag="PKN91_08860"
     CDS             complement(5404..6159)
                     /locus_tag="PKN91_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNS28223.1"
                     /translation="MILEESMENQATAVVENPYGLEALWAQGDFVAKTTLIILALMSL
                     GSWYIILTKMWDQRKLRQSAKVVEKQFWTAPSIKDGVERLKKGDDFRNIAEDGLRAAS
                     HHEGRLTDRIDLHEWITMSLSRSSDTVNGKLNAGLWFLATVGSTSPFVGLFGTVWGIY
                     HALIAIGIAGQASIDKVAGPVGEALIMTAIGLFVAVPAVIGYNWLLRRNKGLQDGLRN
                     FVSDLHAYLVGGARVGNEGGAARAGAPAAAVRK"
     gene            complement(6156..6794)
                     /locus_tag="PKN91_08865"
     CDS             complement(6156..6794)
                     /locus_tag="PKN91_08865"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNS28224.1"
                     /translation="MAAYAHEPTFIPRRIVVFLLIVLFHALLVWALANGLARKVVEVI
                     APPIQTDIIEEIAEDDPPPPPPPPEMERPPVEVPPPEVNIQIPVDTSTSTAITDVTDR
                     PVPMAPPPPPPKAVVRTNPTVDRRSQPTEDYYPPASKRAEEEGVATVRVCVGTNGRVT
                     GTPTVTKSSGHPRLDEAAIRWASRGARFRPGTEDGKPVESCFQFNVRFQLTN"
     gene            complement(7072..8757)
                     /gene="ilvD"
                     /locus_tag="PKN91_08870"
     CDS             complement(7072..8757)
                     /gene="ilvD"
                     /locus_tag="PKN91_08870"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNS28225.1"
                     /translation="MTNGKSKRGDPRAYSRLVVDGPKQAPSRAMLRAVGFSEADFGKP
                     QVGIASTWANVTPCNMHIGELAKEAAAGADAAGGKSVIFNTITVSDGVSMGSPGMRYS
                     LVSREVIADSIETVVGAEGFDGVVAIGGCDKNMPGCAIAIARLDRPAVFVYGGTIRPG
                     VKRRDIVAVFEAVGGHAAGKVSDAELLDVERTSIPGPGSCGGMYTANTMASAMEALGL
                     SLPGSSAQEAVGATKQNDARRAGEAVVRLIGLGIRPSDILSRKAFENAITVTIALGGS
                     TNAVLHLLAIARAARVKLSLDDFTRIGKRVPVLADLRPSGRFLMSELVAIGGIQPLMK
                     RLLDAGLLHGDCMTVTGRTLAENLASVRDYPAGQDIVRSLDHPLKKDSHLVVLYGNCA
                     PEGAVAKISGKEGLRFTGRARVFDGEERATRAILDGKVKAGDVVVIRYEGPRGGPGMR
                     EMLSPTGAIMGRGLGNDVALITDGRFSGGSHGFVVGHVSPEAAVGGPIALLRSGDTIT
                     IDAVARTIDADVSRAEWARRKRAWRPRKPYATAGVLAKYARVVSSASDGAVTD"
     gene            complement(8784..9662)
                     /locus_tag="PKN91_08875"
     CDS             complement(8784..9662)
                     /locus_tag="PKN91_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNS28226.1"
                     /translation="MISIAPRHLLLLLGVTLIWGLNLVVAKVGVGEMPPVMFTTLRFA
                     ILAILTIPYLRWHGEATGAIIMVALLVGGLHFALLFIGLARADNISSVAIATQLGVPF
                     ATLLSIALLGERVRWRRWTGIGCAFLGVCIMGFDPAVFSQLESLAFVIGSAFIGSLGL
                     IAIKRLPRMPALEMQAWIAWISWPMLGVLSLLLEDGQAAALAQAGWTGWGTLLYTAVL
                     ASLFAHTAYFFLVQRYSVTSIAPLTVLSPVFSVVFGVLLLGDTLTPRIVLGGACTLLG
                     VLIITLREKQFTDTGT"
     gene            complement(9659..>9944)
                     /locus_tag="PKN91_08880"
     CDS             complement(9659..>9944)
                     /locus_tag="PKN91_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28227.1"
                     /translation="VAEAEHDPLWPMPLWCGYDDDLASKIADLNNVSAGAFAGSVIGA
                     LFLRRFVTASPRWVHIDLYAWNPKDRPGRPVGAEAQCVRALYRFIRARCG"
BASE COUNT         1689 a         3375 c         3185 g         1695 t
ORIGIN      
        1 gcaggccctg gcggatcgca tcgagcttga tgccgagcga gaacgccatc gccgcggcga
       61 acatcgcgtt ctgcacgttg tgggtcgccc gcccctcgag ggtcgccgga atcaggtggg
      121 tccacagcag cgggatatga ccaccgcggt cgtacagcgt gatcatctgc ccgttcacgc
      181 ccgcctcgag cgcacacgcc cgaccgcccg cacgcacgtg ctcgcgcacg agattgtgct
      241 gcggagtcat cgtcacgtag cagatgctct gcgcgtccgt atagcccgac atgcgcagcg
      301 tcaacggatc gtcggcattc agcaccgcgc agtcggtcgc cacctcgacg accacgcgct
      361 tgatctcggc gagctgctcc agcgtgtcga tgcccttgag gccgagatgg tccgactgca
      421 cgttgagcac ggcgccgaca ttgacctcct gcacgcccat gccggcacgc aacagcccgc
      481 cgcgcgcggt ttcgagcacg gcgagatcga tctgcgggtc ggcgagcacc atgcgcgccg
      541 aaaccggccc ggtcatgtcg ccctgcacgg tgcgctggcc atcgatatac acgccgtcgg
      601 tcgtcgtgag accgggcgtg aagcccgcca tcttcgcgat gtgcgccagc atgcgcgccg
      661 tcgtcgtctt gccgttcgtg ccggtgatcg cggcgatcgg cactcgcgat ggcgcgcccc
      721 gcgggaacag catgtcgatc accggtcccg cgacatcgcg cgaaatgccc tcgctcggcg
      781 acacgtgcat acgaaagccc ggcgcggcgt tcacttcaca gatgccgccg ccgattctgc
      841 ggtagctctc cgcgatattc cgcgagagga aatcgacacc gcccacgtcg agcccgatcg
      901 cccgcacggc gcgcacggcc atatcacggt tgtccgggtg gatcacgtcg gtgacgtcgg
      961 tggccgtgcc tcccgtcgaa agattcgcgg tggagcgcag cggcacgacc tgccccggcg
     1021 caggcaccga ctcggcggtg agtcccgccc gctcgagcat gcggacggcc tgcgcatcga
     1081 gctcgagccg ggtcagcacc ttctcgtggc ccacgccacg gcgcggatcc agattcacga
     1141 ggtcgatgag ttccgcaacg ctgtgcgcgc cgtcgccgac cacctgtccg ggcgtgcgac
     1201 gcgttgccgc cacgagttcc ccgttcacca cgagcagccg gtgatcgtca ccttccagga
     1261 aggtctcgac gatgaccgag cgcgagtgct cgcgggccgc ggcaaagcct gccgcaactt
     1321 cggcaccgct catcaagcgg atcgaaatgc cacggccgtg attaccgttg tacggcttcg
     1381 tgacgaccgg aaaaccgatg cgctgcgccg cccgcactgc ctgctccgcc gtctgcacga
     1441 gctcctggcg cggcaccggc aggccgagtg aggcgaggat gcggttcgtc tcctccttgt
     1501 cactcgcgag ttcgacggcg atgtgcggcg tgcggccggt caccgtcgcc tggatgcgtt
     1561 gctggtactt gccatggccg agctgcacga gcgactggtc gttgagccgc agccacggaa
     1621 tgccgcgcgc ctcggcggcg cgcaccagcg aggccgtcga aggcccgagg gcccgccgct
     1681 gcgcaaagcg gatgaaatca tcgcgcgcgg ccggccagtc ccagccctcg gggacgctgg
     1741 ccgcggtacg cagctgcggc ggcagcagcg aagccagcag gcgcaggccg agttcacccg
     1801 ccgcgacgcc ctcgtcgcgc tgggcaaatt cgtagacgac cgtgtatacg cccggacgac
     1861 cgtcgacgct gcgcgtcttg ccgaacgtga catcctcgcc ggcgacgttc tgcagctcga
     1921 tcgccacatg ctcgagcacg tgaccgagcc aggtcccgtc gccctcgcgc atcctgcgca
     1981 ggaagcctcc cggttcacgg taggagcagc cgtgtccggc gagtccgggc agcgcctcga
     2041 cgaggctgtc gacgaaggcc ggtcccaggc gcacggtggg ccattgctcg agttccccga
     2101 ggtcgagctc gaggcggatg acgggaaaac gggcgtaatg cgagggtcct gcatacaccg
     2161 accgctcgag tacgcgcatc ggctattcct tcagtgcccg cagggccaca gcagaggcga
     2221 tgcgtgtgtg caggttgaag gtcgctccgc tcaccagcag gtgcagcttc agtcccagca
     2281 tgcagatcgg ctggccttca ctgaccagat ccatcgagga gaaactgacg tcggcggcat
     2341 cgaccaccgt gaccccgcca ctgccctcga cttcgagcgt atcgtccgga ccgatgaatg
     2401 ctgcggtgtc ctcgtcgagc ccgatgccga ctgagaacgg attgtaggcg agcgccgtca
     2461 gcaggcggcc gaggcggtcg cgctggcgga aatgctggtc gatcacgaag cggttggtca
     2521 ggccgagacc cggcgcgagg cgcacgctgc ccgagatcgc ggacgaacca tcgtcgccga
     2581 atgcgatcat gtgttcagaa aggatgctcg cgccggcgct cgtgcccccg acggtgaccc
     2641 cgcgcgcatt gcgcccgcga atcagcttcg caaccggtgt gccgccgagc agcgtcgtca
     2701 gtcgcagctg gttgccgccc gtgaagaaga tgccgctcgc ctcgcccagc cggtcgagtc
     2761 gtccctgctc gtggcagtcg cgccgggtat cgaagtccat gaccgctacc cgcgccgccc
     2821 cgagctgccg gaacagctgc tcgtagcggc tcccggtctc gtggaggcgg ctcgccgtcg
     2881 ggatcacgac gatgtcggca tcgctgcccc ctgaaaccgc gacgaagcgg gcgaggatcc
     2941 gcttgtcgtt ctccttgttc tcggcgccgc cgatcggaac gatccagccg cgcgcctcgc
     3001 cgtcgggcac cttgctcggc atgcgcttgt tgcctctcag gcctaccgcc gaaatgcgtg
     3061 taggtgaaat agtgtcacag cgcggccgca aacaaaacaa aacggccggt gggttgcccc
     3121 accggccgtc ccggttcctt tgaagtcctc agccctcagc tctgtgcatg aatcgcccag
     3181 aggctcgcga gccggccata cctggcgtcg gtcttgatcg tgtcgaaggt cttgacggcg
     3241 tcgctcttgt taccgccctt caactgcgag atgccgagca gcagggtggc ttcagatgcg
     3301 tcactcaggc cgcccttcgt gaggccacgc tggatggccg ccgccgcctt gtcatactcg
     3361 ccgtagctca gatagccctt gccgaggccc acgtccgcac caccggtctt ggcgctgccg
     3421 gtctcggttg cgagctttgc gagcgccgcc tggtcggtgg ctgcagactt cttcgccagg
     3481 tcgagcaggc gctggttctt gtccttcgcg cgctggtcgg caaatacgtc cttcgcgaag
     3541 cccttctcga gaatcgagac ggcctcaccc ggcgagccct gctcgatcgc aagctgcgcc
     3601 atctcggtgt agtcctcgct gcggctgagc gcgtcgacat cggccgcgag gcggaacgta
     3661 ttgaacagca ccttgtcgtc ccggtccggc gcgcggaaca gcgagtacat gatgttctgc
     3721 cagtattccg gcgacgggta gtacttgacg aggcgctcga gcgcagcggt cgtgcactcg
     3781 tcatccttct gcttcacaca cgaggaaagg atcagttcga gcgactgctt cttcggggtc
     3841 tgaccacggc ggatctggtc accgacgtag ctgtccatga acttgtgcgt gcccttgaaa
     3901 tcgttctgca cgtagtacgc ctgcccgacc agggtataca gatcggcatc ggcaaagccg
     3961 cccttgatgg cgcgattgcc gtactcgacg gccttgccgt agttcttgtt ctggtagctg
     4021 atctgcgcca gcgcgcgaac gcgggacgcc acgtcctcct gcgcgaggaa gcccgagttc
     4081 aggcccgcct ccagattgcg gccggcttcc gcaaagttgt tgagccgcac atacgcgaag
     4141 ccgcgcatct cggcgatcag gaattggtcg taggcgctct tgcccgacgt ggcatcagcc
     4201 ttgtcgagtt cggcgagcgc gccgcgccag tccttcttgc cgagcgcatc ctgcgccgcc
     4261 ttgagcggct tggccagcgc cttgctgacc gcgggcgcct tctggtcggc cgcaacagcc
     4321 gggccggaaa tgcccactga tccgacggcc agcaaggcca tcgccagcaa cggtgcgagt
     4381 tggagtgtct tcctcatgct catggtcctc tgatccaatg tcgcgacacg gtagcagtgc
     4441 cacggtgtca cgatctcatg tggcggatga acgcagaatg aacgccggcg gggcacgccc
     4501 gccggtggtc acaattcccg gaattccgcc gtattgacga aaccgatctt caacatgcga
     4561 ttgcgctgtg ccgaggcgag cacctgcgcc accgtgtcat agcgcgagcg cgcatcgggc
     4621 cgcaggtgca gttccggctg cgggtccttc tgcgactcct gccggaagta gccctcgagc
     4681 gtagcgatgc tcggcaccac cgtgccgttc cagacgatcg tgccgtcgaa gtcgatctcg
     4741 atatcgatca cctcggggcg cacctccggc aacggctggt cgggctgcgg ctggggcatg
     4801 tccagtttca ccgcgtgcgt catcaccgga atcgtgatga tgaacatgat cagcagcacc
     4861 agcatcacgt cgatgagcgg cgtggtgttc atctccacca tcggttcgtc ttcgccgctc
     4921 gcgctgccca cattcataga catagtcgat atctcctcaa cgcacgccgc gatcgggctc
     4981 ggtgatgaat cccaccttga ccacaccacc gcgctggatc gtgtagatca cgcgtcccac
     5041 cgcttcgaaa cgcgcattac gatccccgcg aatgtggatc tcgggttggg gcacctgcac
     5101 ggccgccgcc ttgacgaact cgagcagcgt ctgtgtatcg ggcacctggc cgttgttcca
     5161 gtacatgttg ccccgctcgt cgaccgccac ggtgatgttc tccgggcgcg tctgcgtggc
     5221 gatgttggtc gctttcggca gctcgaccgg caccgtcttc gtgatcacgg gcacggtgat
     5281 caggaagatg atgaggagca ccagcattac gtccaccaag ggcgtggtgt tgatctccga
     5341 catcgccttc ggttcgtcgt ccgccgagcc ggttccgaca ttcatggcca tgcgctgggt
     5401 tccttacttg cggaccgcag ccgccggggc tccggcgcgg gccgcgccac cctcgttgcc
     5461 gacgcgcgcg ccgcctacca gataggcatg caggtcggag acaaagttcc gcaggccgtc
     5521 ctgcaagccc ttgttgcgac gcagaagcca gttgtagccg ataaccgcgg gcacggcaac
     5581 gaacagaccg atggcggtca tgatcagcgc ctcgccgacg gggccggcca ccttgtcgat
     5641 gctcgcctgg ccggcgatgc cgatcgcaat cagggcgtga tagatgcccc acaccgtgcc
     5701 gaacagaccg acgaagggcg aagtcgagcc gacggtcgcg aggaaccaca gtccggcgtt
     5761 cagcttgccg ttgaccgtgt cggacgagcg cgagagcgac atggtgatcc actcgtgcag
     5821 gtcgatgcgg tcggtcagac gaccttcatg gtgcgatgcc gcacgcagac cgtcctcggc
     5881 aatgttgcgg aagtcgtcgc ccttcttcag gcgctcgacg ccgtccttga tcgacggtgc
     5941 agtccagaac tgcttctcga cgactttggc ggactggcgg agcttccgct ggtcccacat
     6001 cttcgtgagg atgatgtacc aggagcccag cgacatgagc gccaggatga tgagagtggt
     6061 cttcgcgacg aagtcgccct gtgcccagag cgcctccagt ccgtacggat tctctacgac
     6121 tgcggtggct tgattttcca tagattcctc aaggatcaat tggtcagctg gaaacgaacg
     6181 ttgaattgga agcagctctc gaccggtttg ccgtcttcgg tgcctggcct gaagcgtgcg
     6241 ccgcgcgatg cccacctgat cgcagcctcg tcgaggcgcg ggtgaccgga actcttggtg
     6301 accgtgggcg tacccgtgac gcgcccgttg gtgcccacgc agactcgcac ggtggcaaca
     6361 ccctcttctt ctgcgcgctt ggacgccggc gggtagtagt cctcggtcgg ctgtgagcgc
     6421 cgatcgacgg tcggattggt acgcaccacg gccttcggcg gcggcggcgg cggcgccatc
     6481 ggcaccggac gatcggtaac gtcagtaatc gcagtcgaag tactggtatc gaccgggatc
     6541 tggatattca cttccggcgg cggcacctcg accggagggc gctccatttc gggcggcggc
     6601 ggcggcggcg gcgggtcgtc ctccgcgatc tcctcgatga tgtccgtctg gatcggcggc
     6661 gcaatcacct cgacgacctt gcgggccagg ccgttcgcca gcgcccatac gagcagcgca
     6721 tgaaacagca caataaggag gaacacgacg atgcgtcgcg gaatgaatgt cggttcgtgc
     6781 gcgtaggcgg ccatgttttc tcgtggtcgc ggattaattg tccggacaat ccgcagtatt
     6841 ttgcgagcgc ggcacgctat acgcgtttcc cgcccaacgc aacccttcct gccgattatc
     6901 cacccgacgg cgcgtcctgg cgcctgcccg gaagcgccag tcaaggccgc acttaagagc
     6961 caggctgaat cttcctgaaa tcgtcatccc caggggatcg gaactgtaac tgctggcatc
     7021 ttcgcttaca acttctggcg atcgcaatta attttgtcgg ccggcggccg ctcaatcggt
     7081 caccgcacca tcggaggcac tgctgaccac ccgcgcgtac ttggcaagca cgcccgcagt
     7141 cgcatacggc ttgcgcggcc gccacgcacg cttgcggcgc gcccattcgg cacgcgacac
     7201 atcggcatca atggtgcgtg ccacggcatc aatggtgatg gtgtcgccgc tacgcagcaa
     7261 tgcaatcgga ccgcccactg cagcctcggg cgagacatgt ccgacgacga agccgtggct
     7321 cccacccgag aagcgcccgt cggtgatcaa cgcaacatcg ttgccgagcc cgcgccccat
     7381 gatggcgccg gtggggctca gcatttccct cattccgggc ccgccgcgcg gcccttcgta
     7441 acgaatcacc accacgtcgc cggcctttac cttgccatcg aggatcgcgc gtgtggcccg
     7501 ctcctcgccg tcgaacaccc gcgcccgccc ggtgaagcgc aggccctcct tgccggaaat
     7561 cttggcgacg gcgccctcgg gcgcgcagtt accataaagc acgaccaggt ggctgtcctt
     7621 cttcaaggga tgatccagcg accggacgat gtcctgtccc gctggatagt cgcgcacgct
     7681 ggccagattc tcggcgaggg tacgcccggt gacggtcatg cagtcgccgt gcagcaggcc
     7741 cgcatcgagc aggcgcttca tcaacggctg gatgccgccg atggcgacca gctcggacat
     7801 caggaagcgg ccacttggac gcaggtcggc cagcaccggt acgcgcttac cgatgcgcgt
     7861 gaagtcgtcg agcgagagtt tcacgcgcgc ggcgcgcgcg atcgcgagca ggtgcagaac
     7921 cgcgttggtc gaaccgccga gcgcgatcgt cacggtgatc gcattctcga acgccttgcg
     7981 cgagaggatg tccgacggcc ggattccgag cccgatgagg cgcaccaccg cctcgccggc
     8041 gcggcgcgcg tcattctgtt tggtggcgcc cacggcctcc tgggcagaac tgcccggcaa
     8101 tgacagtccg agcgcctcca tcgcggatgc catggtgttg gcggtgtaca tgccaccgca
     8161 gctgccagga cccgggatcg atgtgcgctc gacatcgagc agctcagcgt cgctcacctt
     8221 gcctgcggca tggccgccga cggcctcgaa caccgcgacg atgtcgcggc gcttcacgcc
     8281 ggggcgaatc gtgccgccgt agacgaacac ggccgggcga tcgaggcgcg cgatcgcgat
     8341 cgcgcagccg ggcatgttct tgtcgcagcc gccgatcgcg accacgccgt cgaagccctc
     8401 ggcacccacc acggtctcga tcgagtcggc gatgacttcg cgcgacacca gcgagtagcg
     8461 catgccgggc gagcccatcg acacgccgtc ggagacggtg atcgtgttga agatcacgct
     8521 cttgccgccc gcggcgtcgg cgcccgcggc agcctccttc gcaagctcac cgatatgcat
     8581 gttgcatggc gtgacattcg cccaggtcga cgcgatgccg acctgcggct tgccgaagtc
     8641 cgcttccgaa aagccgaccg cacgcagcat cgcgcgcgac ggcgcctgct tcgggccgtc
     8701 caccacgagg cgcgaatatg cacgcgggtc gccccgcttc gattttccgt tggtcatgtc
     8761 tctcgtctct tcgttgactg caatcaggtg cccgtatcgg tgaactgctt ctcgcgcagc
     8821 gtgatgatca ggacaccaag gagcgtacac gcaccgccga gcacaatgcg gggcgtcaga
     8881 gtatcgccga gcagcagcac gccgaatacc accgaaaaca ccggcgagag cacggtcagc
     8941 ggcgcgatac tcgtgaccga atagcgctgc accaggaaga agtatgccgt gtgcgcgaac
     9001 aggctggcga gtaccgcggt gtagagcagc gtaccccagc cggtccagcc ggcctgcgcg
     9061 agcgcggcgg cctggccgtc ttcgagcagc agcgacagca cgccgagcat cggccagctg
     9121 atccaggcaa tccacgcctg catttcgagc gccggcatgc gcggcagccg tttgatggcg
     9181 atcaagccaa gcgacccgat gaatgccgag ccgatcacga aggccaggct ttcgagctgg
     9241 ctgaacaccg ccgggtcgaa acccatgatg catacgccga ggaacgcgca gccgatgccg
     9301 gtccacctgc gccaacgcac gcgctcgccg agcagcgcga tcgacagcag ggtcgcgaat
     9361 ggcacgccga gttgcgtcgc gatggcgacc gaggagatgt tgtcggcgcg ggcgagcccg
     9421 atgaacagca acgcgaagtg caggccaccg acgagcagcg ccaccatgat gatcgcgccg
     9481 gtcgcctcgc cgtgccagcg caggtacgga atcgtgagaa tcgcgaggat cgcaaagcgc
     9541 agcgtcgtga acatcaccgg cggcatctcg ccgacgccga ccttggcgac cacgaggttc
     9601 aacccccaga tcagcgtgac accaaggagc agcagcaggt ggcgtggcgc gatcgagatc
     9661 atccgcagcg tgcgcggatg aagcggtaca gcgcgcgcac gcactgcgcc tcggcgccga
     9721 cgggcctccc tggccgatcc ttcggattcc aggcatacag gtcgatgtgc acccagcgcg
     9781 ggctcgccgt cacgaagcga cgcaggaaca gcgcgccgat gaccgagccg gcgaaggcgc
     9841 ccgcggaaac attgttgaga tcggcgatct tgctcgcaag atcgtcgtcg tagccgcacc
     9901 acagcggcat cggccacagc gggtcgtgtt cggcttccgc cacg
//
LOCUS       DAOQVY010000089         9875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_7687_length_9875_cov_4.921792, whole genome shotgun sequence.
ACCESSION   DAOQVY010000089 DAOQVY010000000
VERSION     DAOQVY010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9875
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7687_length_9875_cov_4.921792"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /gene="gspF"
                     /locus_tag="PKN91_08885"
     CDS             complement(<1..1088)
                     /gene="gspF"
                     /locus_tag="PKN91_08885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HNS28228.1"
                     /translation="MGAFEYTALDTTGREQKGVLEGDTAKHVRQLLRERGLLPVTVTE
                     AAREEARRQRSLSLRRGVSAADLSLMTRQLATLTKAGLPLEEALLAVSQQSEKPRVQS
                     ILLGVRSRVMEGHTLASGFAEFPRVFPEIYRATVTAGEQSGHLDEVLERLADFTESRE
                     LMRQKVLGAMLYPIVLTVMCFGIVTGLLVYVVPKVIEVFDTGKAQLPLMTRALIAVSD
                     FLHDWGLWALALIIVAAIAFSRWLRNPEARGRFHRFQLRLPLLGKLVQGFNTARFTRT
                     LSILTAAAVPVLEALRIAGEVVTNIPMRNAVTEAGQRVREGAPIGRSLAHSKLFPPMT
                     IHLISSGESSGELESMLERAAISQEREL"
     gene            complement(1116..2624)
                     /gene="gspE"
                     /locus_tag="PKN91_08890"
     CDS             complement(1116..2624)
                     /gene="gspE"
                     /locus_tag="PKN91_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HNS28229.1"
                     /translation="MNEAATAASTPVIARAPERRLSFAFAKRHGVLVRHVTDGVADCA
                     YRGDVTPLAIAEVRRHLRMPVKLARVGDEEFDTLLRLTYEAGSDAIQAAEGLDGSVDL
                     AHLALDLPEQADLLEADDDAPIIRLINALLTQAVKENASDIHIEPFENRLVIRFRVDG
                     VLREVLQSRRAVAPLVVSRIKVMSKLDIAEKRLPQDGRISLRVAGRAVDVRVSTIPSG
                     HGERVVLRILDKQAGRLNLDSLGMDPKTQALMDELIHKPHGILLVTGPTGSGKTTTLY
                     AALERLNDNTRNIMTVEDPIEYYIDGIGQTQVNTKVEMTFARGLRAILRQDPDVVLVG
                     EIRDLETAQIAVQASLTGHLVLSTLHTNTAAGAVTRLRDMGIEPFLLSSSLIGVVAQR
                     LVRVLNPDTKQPFRAGEYERRLLGLGPDDESPILYRPGNEIGQGYRGRSGIYEVIVVD
                     DQMRTMIHDGAAEHELERYARTITPSIRDDGLNRVLRGETTMEEVLRVTRED"
     gene            complement(2621..4714)
                     /gene="gspD"
                     /locus_tag="PKN91_08895"
     CDS             complement(2621..4714)
                     /gene="gspD"
                     /locus_tag="PKN91_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HNS28230.1"
                     /translation="MTPAVLQRARVAAFAALLTLAGAASAQQQGPRITPNFKDADITQ
                     IVEAVSAATGKNFIIDPRVGRQQVTMLSTTPMTPEAFYQAFLSILQVHGFVAMQTGDI
                     IKIIPDANARQFPSNDLPDRVSSTSDEIVTQVIAVRNVSAAQLVPILRPLIPQYGHLA
                     AYPASNMLIISDRANNVNRIVRIIRRIDQAGDAEIEVIPMQNASATEVVRVVTQLVQG
                     AASAEGGAGVGASLKLVADDRSNSVLISGEQAARLRVKALIAHLDTPLESGGDTQVRY
                     LRYSDAEKVASKLKEQITGVAAAAGGAAGATAGGAAAQAERNSTIWADPENNALVITA
                     PPKTMRSLMGIIDKLDIRRAQVLVQAVIVEVRADKTAELGVNWAIDGSDDNFAVGSFV
                     QPIAGGSIVDIIRGIDDPSTLTPGAITGTTIGGGRVGSGSTDFAAVLRAIRNDSDTNI
                     IATPSIVTLDNQEAQIKVAQEVPFITGQYTNTGGTDGALNPFQTVQREEVGIILKITP
                     QINEGSAVILKIEQEASTLSTSTASAVDLITNKRVINTTLLVEDGGTIVLGGLIQDED
                     RRGEQRVPFLGRIPLIGELFRVRQATRIKTNLMVFIQPKILRDGVQASFETDAKYNYI
                     RGEQQGLGRKFEILPLLPGEKKAVLPELPPPVLREPDATDKTAEDLAREAAEPRPASP
                     PPAAVPPATVPPETP"
     gene            complement(4722..5735)
                     /gene="gspC"
                     /locus_tag="PKN91_08900"
     CDS             complement(4722..5735)
                     /gene="gspC"
                     /locus_tag="PKN91_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HNS28231.1"
                     /translation="MTTTVLQGVPSPDALKGLLIAHGPRIATWALALGLGVQGAVLVT
                     DIAGSDPAAPIDIEPMPVPARFDVAALVGAQLFGQAEVSPGTGNAADAPPTSMALVLT
                     GVIANTDPKTGIAILGESAATAKVIMVGDTVPGGARLDSVFPDRVVLERAGALESLLL
                     PQQHAAGMASVAPAPMPTQRPTTADVQILDRMRRMIETDPGLIGDVMRPQPVFAEGKQ
                     RGYRVYPGRNRAAFSRLGLRPGDLVTAINGTPLDDPARGGEIFNTLGSSAQARVTVMR
                     SGREQELTLDMAQIAAEAEQLGSPPPLPDAAQAALDGGVEIADESQLEEQPVSPPTEN
                     RGD"
     gene            5852..6172
                     /gene="clpS"
                     /locus_tag="PKN91_08905"
     CDS             5852..6172
                     /gene="clpS"
                     /locus_tag="PKN91_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004879763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HNS28232.1"
                     /translation="MADIEPTRPDGGVLVEEATPKLKRPPLFQVILLNDDYTPMEFVV
                     DVLERFFGMNRPQATRVMLEVHMKGKGVCGVFSYEIAETKVAQVTTYARDNQHPLMCT
                     LEEA"
     gene            6172..8430
                     /gene="clpA"
                     /locus_tag="PKN91_08910"
     CDS             6172..8430
                     /gene="clpA"
                     /locus_tag="PKN91_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HNS28233.1"
                     /translation="MLSSELEYCLNDAFREARDSRHEFLTVEHLLYSIVETTRVREIL
                     RACGADIAKLKPELHAHIQETTPRLPEGDDREVQPTLGFQRVLQRAVFHVQSSGGKEV
                     GVGNALVAIFSEKQSHAVYLLEKQGVTRLDVVNYVSHGLSKVAEEKPEVGGEQGDGEG
                     EGGGALEKYTVNLNREAQAGRIDPLIGRQLEVERTIEILCRRRKNNPLYVGEAGVGKT
                     AIAEGLARLIVEGKVPDVLAESTIYALDMGALVAGTKYRGDFEKRLKGVITELKKHPG
                     AILFIDEIHTVIGAGAASGGVMDASNLIKPVLTSGEIRCIGSTTYQEFRGIFEKDHAL
                     ARRFQKIDVVEPSVAETIDILRGLRSRFEEHHAITYTDEALRAAAELASRHINDRHLP
                     DKAIDVVDEAGARLRLKPAGERELVVDVPQIEDVVARIARIPAKSVSTSDREVLRNLE
                     RNLKLVIFGQDNAIETLAAAIKMARSGLGDQRKPTGSFLFAGPTGVGKTEVTRQLALA
                     LGVEFIRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAISKHPHCVLLLDEIEKA
                     HPDVFNLLLQVMDHGTLTDNNGRKADFRHVTIVMTTNAGAQEMSRPSIGFTQQDNASD
                     GMEAVRRLFSPEFRNRLDAIIQFAPLDAATIERVVEKLLVEVEAQLEQKRVMLTIDEA
                     ARRWIAQKGYDPKMGARPMARLIQDHIKRPLAEELLFGRLVNGGQVRVSLAADGDGLS
                     LESAPLEEPVPA"
     gene            complement(8450..8668)
                     /gene="infA"
                     /locus_tag="PKN91_08915"
     CDS             complement(8450..8668)
                     /gene="infA"
                     /locus_tag="PKN91_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNS28234.1"
                     /translation="MSKEDAIQMEGEVIETLPNTTFRVQLKNGHVVTAHISGKMRKNY
                     IRILTGDQVTVELTPYDLSKGRIVYRGR"
     gene            complement(8714..9409)
                     /gene="aat"
                     /locus_tag="PKN91_08920"
     CDS             complement(8714..9409)
                     /gene="aat"
                     /locus_tag="PKN91_08920"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNS28235.1"
                     /translation="MKRITWLSPADEPEWFPALEQALDEPAGLLAAGGDLGPRRLLAA
                     YRRGIFPWYSPGQPILWWAPDPREVLFPGEFRRRRSLDKVIRNRGFTTTFNRDFEAVV
                     DSCAAPRDRERERGSWITPEMRAAYVTLAGLGYAHSIETWREGRLVGGLYGVALGSVF
                     FGESMFSRERDASKTALAALVESCPARGIELIDCQLASTHLRSLGSRPLPRAEFSALL
                     ARLIVGEKPQHWT"
     gene            complement(9406..>9875)
                     /locus_tag="PKN91_08925"
     CDS             complement(9406..>9875)
                     /locus_tag="PKN91_08925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS28236.1"
                     /translation="RDTFLRHGHLPYLTREFFTHAAQRLGEALMVKAAMWRDRLVACA
                     IFFRSNATLYGRYWGAAADFHSLHFETCYHQGIEYCIEQGLAHFEPGTQGEHKVARGF
                     EPALTRSAHFIVDAQFRAAIGAHLEREGRAVDQYAAEVAMHLPFRQALRGPGR"
BASE COUNT         1549 a         3213 c         3486 g         1627 t
ORIGIN      
        1 tcgagttcgc gctcctggct gatggccgcc cgctcgagca tggactcgag ctcgccgctc
       61 gattcgccgc tcgaaatcag gtggatcgtc atcggcggaa agagcttgct gtgggcgagc
      121 gagcggccga tcggcgcgcc ctcccggacc cgctgccccg cctcggtgac cgcgttgcgc
      181 atcgggatat tggtgaccac ttcaccggcg atgcgcagcg cctcgaggac cggtaccgcc
      241 gccgcggtca ggatgctcag cgtgcgtgtg aaccgggcgg tgttgaagcc ctgcaccagc
      301 ttgccgagga gcggcaggcg cagctggaag cggtggaaac ggccgcgcgc ctcgggattg
      361 cgcagccagc gactgaaggc gatggcggcc actatgatga gggcgagcgc ccatagcccc
      421 cagtcgtgca ggaaatcgct gaccgcgatc agcgcgcggg tcatcagcgg caattgcgcc
      481 ttgccggtgt cgaagacctc gatcaccttc ggcacgacat acaccagcag gcccgtgacg
      541 atgccgaagc acatgaccgt cagcacgatc gggtagagca tcgcgccgag caccttctgg
      601 cgcatcagct cacggctctc ggtgaaatcc gcgagtcgct cgagcacctc gtcgagatgc
      661 cccgactgct cgccagcggt caccgtggcg cgatagatct ccgggaagac acgcggaaac
      721 tcggcgaagc cgctcgcgag cgtatggcct tccatcacgc gcgagcgcac gccgagcagg
      781 atgctctgca cgcgcggctt ctcggactgc tgcgagaccg cgagcagggc ctcctcgagc
      841 ggcaggccgg ctttcgtgag ggtggcgagc tggcgggtca tcagcgacag gtcggcggcc
      901 gagacgccgc ggcgcaggct gagactgcgc tggcggcgcg cttcctcgcg ggccgcctcg
      961 gtgacggtga cgggcagcag gccgcgctcg cgcagcagct ggcgcacgtg cttggcggtg
     1021 tcgccctcga ggactccctt ctgctcgcgg ccggtggtgt cgagggcggt gtattcgaac
     1081 gcgcccatgg ctagcgggcg gtcccgggtc ccgtatcagt cttcacgggt cacccgcagg
     1141 acttcctcca tggtggtctc gccgcgcagc acgcggttca ggccatcgtc cctgatcgag
     1201 ggggtgatcg tgcgggcgta gcgctcgagc tcgtgctcgg ccgcgccgtc gtggatcatc
     1261 gtgcgcatct ggtcgtcgac gacgatcacc tcgtagatgc cgctgcggcc ccggtacccc
     1321 tggccgatct cgttgcccgg ccggtagagg atcggcgact cgtcgtcggg tccgagaccc
     1381 agcaggcgcc gctcgtactc accggcccgg aacggctgtt tcgtgtcggg gttcagcacc
     1441 cgcacgaggc gctgggcgac gacgccgatc aggctcgagg acagcaggaa gggctcgatg
     1501 cccatgtcgc gcaggcgcgt gacggcaccc gccgccgtgt tggtatgcag cgtggagagc
     1561 acgaggtggc ccgtgagact cgcctgtacg gcgatctgcg cggtctcgag gtcgcggatc
     1621 tcgccgacca gcacgacgtc cgggtcctgg cgcaggatcg cgcgcaggcc gcgcgcgaag
     1681 gtcatctcca ccttcgtgtt gacctgcgtc tggccgatgc cgtcgatgta gtactcgatc
     1741 ggatcctcga ccgtcatgat gttgcgcgtg ttgtcgttca ggcgctcgag cgcggcgtac
     1801 agcgtcgtgg tcttgcccga accggtgggg ccggtgacga gcaggatgcc gtgcggcttg
     1861 tggatcagct cgtccatcag cgcctgggtc ttcgggtcca tgccgagcga gtcgagattc
     1921 agtcgcccgg cctgcttgtc gaggatacgc agcaccaccc gctcgccgtg gcccgacggg
     1981 atcgtcgaga cgcgcacgtc gaccgcgcgc ccggcgacgc gcagcgaaat gcgcccgtcc
     2041 tgcggcaggc gcttctcggc gatgtcgagt ttcgacatga ccttgatgcg cgatacgacc
     2101 aggggcgcca cggcgcgccg cgactgcagc acttcgcgca gcacgccgtc gacgcggaag
     2161 cggatcacga ggcggttctc gaacggctcg atgtggatgt cggaggcgtt ctccttcacc
     2221 gcctgcgtga gcagcgcgtt gatcaggcgg atgatcggcg cgtcgtcgtc ggcctcgagc
     2281 aggtccgcct gctcgggcag gtcgagcgcg aggtgcgcga ggtcgacgct gccatcgagt
     2341 ccctcggcgg cctgaatggc gtcggagccc gcctcgtagg tgagccgcag cagcgtgtcg
     2401 aattcctcgt cgccgacgcg cgcaagcttg accggcatgc gcaggtgtcg gcgaacctcg
     2461 gcgatggcca gcggcgtcac atcgccccgg taggcgcagt cggcgacccc gtcggtgacg
     2521 tgccggacga gcacaccgtg gcgcttggcg aacgcaaacg agaggcggcg ttccggcgca
     2581 cgcgcgatga cgggggtaga ggccgcggtg gccgcttcgt tcacggcgtc tccggtggca
     2641 cggtcgcggg tggcacggcg gctggcggcg gcgatgccgg ccggggctcg gcggcttcgc
     2701 gggccaggtc ctcggcggtc ttgtccgtgg catcgggctc gcgcagcact ggcggcggca
     2761 gttcgggcag cacggccttc ttctcgccgg gcagcagcgg cagtatctcg aacttgcgcc
     2821 cgaggccctg ctgctcgccg cggatgtagt tgtacttggc gtcggtctcg aacgaggcct
     2881 gcacgccgtc acgcaggatc ttcggctgga tgaagaccat caggttggtc ttgatccgcg
     2941 tcgcctggcg taccctgaag agctcgccga tgagcggtat ccgcccgaga aacggcacgc
     3001 gctgctcgcc gcggcgatcc tcgtcctgga tcagcccgcc cagcacaatc gtgccgccgt
     3061 cctcgaccag cagcgtggtg ttgatgacgc gcttgttggt gatcaggtcg acggcgctcg
     3121 ccgtgctggt cgagagcgtg gaggcttcct gctcgatctt caggatcacg gcgctgccct
     3181 cgttgatctg cggcgtgatc ttcaggatga tgccgacttc ctcgcgctgc acggtctgga
     3241 acgggttcag cgcgccgtcg gtgccaccgg tgttggtgta ctggccggtg atgaacggca
     3301 cttcctgtgc caccttgatc tgcgcttcct ggttgtcgag tgtcacgatc gatggcgtgg
     3361 cgatgatgtt ggtgtcgctg tcgttcctga tcgcgcgcag caccgcggcg aagtcggtgc
     3421 tgccgctgcc gacgcggccg ccgccgatcg tcgtgccggt gatggcgccc ggggtcaggg
     3481 tcgacggatc gtcgataccg cggatgatgt cgacgatgct gccgcccgcg atcggctgga
     3541 cgaagctgcc gaccgcgaag ttgtcgtccg agccgtcgat ggcccagttg acgccgagtt
     3601 cggcggtctt gtccgcgcgc acttcgacga tcacggcctg cacgagcacc tgcgcgcggc
     3661 ggatgtcgag cttgtcgatg atccccatca ggctgcgcat cgtcttcggt ggcgccgtga
     3721 tcacgagcgc gttgttctcc gggtcagccc agatcgtcga attgcgctcg gcctgggccg
     3781 ccgcgccgcc ggccgtggcg ccggccgcgc caccggcagc ggccgccact cccgtgatct
     3841 gctccttcag tttgctggcg accttctcgg catcggagta gcgcaggtag cgcacctgcg
     3901 tgtcgccgcc ggattcgagc ggcgtgtcga gatgcgcgat cagcgccttg acgcgcaggc
     3961 gggcggcctg ctcgccgctg atcagcacgc tgttcgagcg atcgtccgcg accagcttga
     4021 gcgaggcgcc gacgccggca ccgccttcgg cgctcgccgc cccctgcacg agctgggtca
     4081 cgacgcgcac cacttcggtg gccgacgcat tctgcatcgg gatcacctcg atttcggcgt
     4141 cgcccgcctg gtcgatgcgc cggatgatgc gcacgatgcg gttcacgttg ttcgcgcggt
     4201 ccgaaatgat cagcatgttc gaggcgggat acgccgcgag atgcccgtac tgcgggatca
     4261 gcggccgcag gatcggcacg agctgcgccg cgctcacgtt cctcaccgcg atcacctggg
     4321 tgacgatctc gtccgaggtc gagctgacgc ggtcgggcag gtcgttcgac gggaactggc
     4381 gggcgttggc gtcggggatg atcttgatga tgtcgccggt ctgcatcgcg acgaagccgt
     4441 gcacctgcag gatcgacagg aacgcctggt aaaacgcttc cggtgtcatc ggcgtggtgg
     4501 acagcatggt gacctgctgg cggccgacgc gcggatcgat gatgaagttc ttgcccgtcg
     4561 ccgcgctcac cgcctcgacg atctgcgtga tgtcggcgtc cttgaagttc ggcgtgatgc
     4621 gcgggccctg ctgctgcgcg ctcgcggcgc cggcgagggt gaggagtgcc gcaaacgcgg
     4681 caacgcgcgc gcgctggagt actgcaggtg tcatggcagg cttagtctcc acggttttcg
     4741 gtcggtgggc tgaccggctg ctcttcgagt tgggattcgt cggcaatctc gacgccgccg
     4801 tcgagcgcgg cctgggccgc atcgggcagc ggcggagggc tgccgagctg ctcggcttcg
     4861 gctgcgatct gggccatgtc gagcgtcagc tcctgttcgc gcccgctgcg catcaccgtc
     4921 acgcgcgcct gggccgaact gccgagggtg ttgaagatct ccccgccgcg ggcgggatca
     4981 tcgagcggcg tgccgttgat cgcggtgacg agatcgcccg gccgcaggcc gaggcgcgaa
     5041 aaggctgcgc ggttgcgccc aggatagacc cggtaaccac gctgcttacc ctcggcgaac
     5101 accggctgcg ggcgcatcac gtcgccgatc agcccggggt cggtctcgat cattctgcgc
     5161 atgcgatcga ggatctgcac gtcggcggtt gtgggccgct gggtgggcat cggcgccggc
     5221 gcaacggacg ccatgccggc ggcgtgctgc tggggcagca gcagcgattc gagcgcgccg
     5281 gcgcgctcga gcaccacgcg gtccgggaac accgaatcga gcctggcacc gcccggcaca
     5341 gtgtcgccca ccatgatcac cttggcggtg gcggcgctct cgccgaggat tgcgatgccg
     5401 gtcttcggat cggtattggc gatcacgccg gtgagcacga gcgccatgct ggtcggcggc
     5461 gcgtcggcgg cattgccggt gccgggtgac acctcggcct ggccgaaaag ctgggcgccg
     5521 accagtgccg cgacgtcgaa ccgggccggc acaggcatcg gttcgatgtc gatcggggcg
     5581 gcgggatcgg atccggcgat gtcggtcacc agcaccgcgc cctgtacgcc gagcccgagc
     5641 gcgagcgccc aggtcgcgat ccgtggcccg tgcgcgatca gcagcccttt caaggcatct
     5701 ggcgagggca cgccctgcag cacggtcgtg gtcatccgcc taatcatacg ggggtgtcga
     5761 tagcgctcac aagcgactgt ttccgcaaga aatcgtgacc ccctgtatcc cgcccttgtg
     5821 ttgccatccg gcgacccata taaaaggtca aatggctgac atcgagccca cacggccgga
     5881 cggcggcgta ctcgtcgagg aagcgacccc caagctgaaa cgaccgccgc tgttccaggt
     5941 catattactg aacgatgatt acacgccgat ggaattcgtc gtcgacgtgc tggagcggtt
     6001 cttcggcatg aatcgcccgc aggcgacgag ggtgatgctg gaggtgcaca tgaagggcaa
     6061 gggcgtttgc ggcgtcttca gctacgaaat cgccgaaacc aaggtggcgc aggtcaccac
     6121 ctacgcgcgg gacaaccagc acccgctgat gtgcacgctg gaggaggcct gatgctgtcg
     6181 tccgaactcg aatattgcct gaatgatgcc tttcgcgaag cgcgcgactc gaggcatgag
     6241 ttcctgacgg tcgagcacct gctctacagc atcgtcgaga ccacccgcgt gcgcgagatc
     6301 ctgcgcgcct gcggcgccga catcgcgaag ctgaagccgg agctgcacgc ccacatccag
     6361 gagacgaccc cgcggctgcc cgagggcgac gatcgcgagg tgcagccgac gctcggcttc
     6421 cagcgcgtgc tgcagcgggc ggtgttccac gtgcagtcga gcggcggcaa ggaggtcggc
     6481 gttggcaatg cgctggtcgc gatcttcagc gagaagcagt cgcacgcggt ctacctgctc
     6541 gagaagcagg gcgtcacgcg cctcgatgtc gtcaattacg tctcgcatgg gctttccaag
     6601 gtcgccgagg agaagccgga ggtggggggc gagcagggcg atggcgaggg cgagggcggt
     6661 ggagcgctcg agaagtacac ggtgaacctg aaccgcgaag cccaagcggg ccgtatcgat
     6721 ccgctgatcg gccgtcagct cgaggtcgaa cgcaccatcg agatcctgtg ccgccgccgc
     6781 aagaacaacc cgctgtacgt cggcgaggcc ggcgtcggca agaccgccat cgccgagggg
     6841 ctcgcgcggt tgatcgtcga gggcaaggtg cccgacgtgc tcgccgagag cacgatctac
     6901 gcgctcgaca tgggcgcgct ggtcgccggc accaagtacc gcggcgattt cgagaagcgc
     6961 ctgaagggcg tgatcaccga gctgaagaag catccgggcg cgattctgtt catcgacgag
     7021 atccacaccg tgatcggcgc gggcgccgcg tcgggcggcg taatggacgc gtccaacctc
     7081 atcaaaccag tgctgacgag cggcgagatc cgctgcatcg gctcgaccac ctaccaggaa
     7141 ttccgcggca tcttcgagaa ggaccacgcg ctcgcgcgcc gcttccagaa gatcgatgtc
     7201 gtcgagccga gcgtggccga gacgatcgac atcctgcggg ggctcaggtc gcgcttcgag
     7261 gaacatcacg ccatcaccta taccgacgag gcgctgcggg ccgcggcgga actggcatcg
     7321 cgccacatca acgaccgcca cctgcccgac aaggcgatcg acgtggtcga cgaggccggc
     7381 gcgcgcctgc ggctgaagcc tgccggggag cgcgagctcg tggtcgacgt gccgcagatc
     7441 gaggacgtgg tggcgcgcat cgcgcgcatc ccggccaaga gcgtctccac cagcgatcgc
     7501 gaggtgctgc gcaatctcga gcgcaacctc aagctggtga tcttcgggca ggacaatgcg
     7561 atcgagacgc tggcggcggc catcaagatg gcccgctcgg gactcggcga ccagcgcaag
     7621 ccgaccggca gcttcctgtt cgcgggcccg accggtgtcg gcaagaccga ggtcacgcgc
     7681 cagctcgcgc ttgcgctcgg cgtcgagttc atccgcttcg acatgtccga atacatggag
     7741 cgccacaccg tgtcgcggct gatcggtgcg ccgcccggct acgtcggctt cgatcagggc
     7801 ggactgctga ccgaggcgat cagcaagcac ccgcactgcg tgctgctgct cgacgaaatc
     7861 gagaaggccc acccggacgt gttcaacctg ctgctgcagg tcatggacca cggcacgctg
     7921 accgacaaca acggccgcaa ggcggacttc cgccacgtca ccatcgtgat gacgaccaat
     7981 gccggtgcgc aggagatgag ccggccgtcg atcggcttca cgcagcagga caacgccagt
     8041 gacggcatgg aagcggtgcg gcggctgttc tcacccgagt tccgcaaccg gctcgacgcg
     8101 atcatccagt tcgcgccgct cgatgccgcc acgatcgagc gcgtggtcga gaagctgctg
     8161 gtcgaggtcg aagcgcagct cgagcagaag cgtgtgatgc tcacgatcga cgaggcggcg
     8221 cgccgctgga tcgcgcagaa gggctacgac ccgaagatgg gtgcacggcc gatggcgcgg
     8281 ctgatccagg accacatcaa gcgcccgctc gccgaggaac tgctgttcgg cagactggtg
     8341 aacggcggac aggtgcgcgt ctcgctggcg gccgacggcg acggactgtc gctcgagagc
     8401 gcgccactcg aggagccggt gccggcctga ggcggcggag gcgcgcggct cagcggccgc
     8461 gatagacgat tcggcccttg ctgagatcgt agggcgtgag ttcgaccgtg acctggtcgc
     8521 cggtgaggat gcggatgtag ttcttgcgca tctttcccga gatatgcgca gtgacgacgt
     8581 gtccgttctt cagctggacc cggaaggtcg tgttcggcaa agtctcgatg acttcgccct
     8641 ccatctgtat ggcgtcttct ttggacatgc agtttccgtt gaaaggcgcg cggattctgc
     8701 ataaagcggc ccctcaggtc cagtgttgcg gcttttcccc cacaatgagg cgcgcgagca
     8761 gcgccgagaa ttccgccctc ggcagcgggc ggcttcccag gctgcgcaag tgcgtcgagg
     8821 cgagctggca gtcgatcagc tcgatgccgc gcgccggaca ggattcgacc agcgcggcca
     8881 gcgcggtctt tgaggcatcg cgttcgcgcg agaacatcga ttcgccgaag aacacgctcc
     8941 cgagcgcaac gccgtagagg ccgccgacga gtcgcccctc gcgccaggtt tcgatgctgt
     9001 gcgcgtagcc gaggccggcg agcgtcacgt aggcggcacg catctcgggc gtgatccagc
     9061 tgccgcgctc gcgctcgcgg tcgcgcggcg cggcgcagga gtcgaccacg gcctcgaaat
     9121 cacgattgaa tgtagtcgta aagcctcggt ttctaatgac tttatcgagg ctcctgcggc
     9181 ggcgaaactc gccgggaaag agcacctcgc ggggatcggg tgcccaccag agaatcggct
     9241 ggcccggcga gtaccaggga aagatgccgc gccggtaggc cgccagcagc cggcgcggac
     9301 cgaggtcgcc gcccgccgcc aacaggccgg cgggttcgtc gagtgcctgc tcgagagcgg
     9361 gaaaccactc gggctcgtct gcgggcgaca gccaggtgat gcgcttcatc gccctgggcc
     9421 gcgcagcgcc tggcggaacg ggaggtgcat ggcgacttcg gcggcgtact ggtctacggc
     9481 gcgaccctcg cgctcgaggt gcgcgccgat cgccgcccgg aactgcgcat cgacgatgaa
     9541 gtgcgcggag cgggtcagcg caggctcgaa gccgcgggcg accttgtgct cgccctgagt
     9601 gccgggttcg aagtgggcga ggccctgttc gatgcagtac tcgatgccct ggtggtagca
     9661 ggtctcgaag tgcaggctgt ggaagtccgc ggcggcaccc cagtagcgcc cgtacagcgt
     9721 ggcgttcgag cgaaagaaaa tcgcgcatgc gaccagtctg tcacgccaca tcgcggcctt
     9781 gaccatcagc gcctcgccga ggcgctgcgc ggcatgggtg aagaactcgc gcgtcaggta
     9841 gggcaggtgt ccgtggcgca ggaaggtatc tcggt
//
LOCUS       DAOQVY010000090         9869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_7692_length_9869_cov_5.867944, whole genome shotgun sequence.
ACCESSION   DAOQVY010000090 DAOQVY010000000
VERSION     DAOQVY010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9869
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7692_length_9869_cov_5.867944"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PKN91_08930"
     CDS             complement(<1..401)
                     /locus_tag="PKN91_08930"
                     /inference="COORDINATES: protein motif:HMM:NF018772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HNS28237.1"
                     /translation="MLVRSGAALLPADDDSRDRIARMPKGQAVRAEVKRMRNLAHHRK
                     FFALVEFVADRHPVYDNREKALVAVKVAAGHVDFLPNPETGELVAVPRSIAFDAMEQD
                     DFEEFYERAVQGVLDHIVPQADREEVDLWVD"
     gene            complement(406..792)
                     /locus_tag="PKN91_08935"
     CDS             complement(406..792)
                     /locus_tag="PKN91_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28238.1"
                     /translation="MTRWTNDQLAEHLDRIKAPPPAVEPAAPKFKSKLEAEYAQMLEA
                     QARAGLIKDWMYEGITVRLADGVRFTVDFNVIALDGSLSMVETKGFLREAARVRLLVA
                     SRMFPMWSWFMVRKVKGAFNVEAIRG"
     gene            complement(789..1085)
                     /locus_tag="PKN91_08940"
     CDS             complement(789..1085)
                     /locus_tag="PKN91_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28239.1"
                     /translation="MSRYAKASADVGGTAGEPAYTGCGAYGCPMPGALSDSTHGSDRW
                     TCRFHFGVASREWDRITTSIRQRLGAGIPLGPTEQTPTVKAMLERVRRPVVGAE"
     gene            complement(1082..1747)
                     /locus_tag="PKN91_08945"
     CDS             complement(1082..1747)
                     /locus_tag="PKN91_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28240.1"
                     /translation="MRDDDKRAFAEALRAVADYYDRKLSADTVAIYWNGLRDLAIEQV
                     RGAINAHVQDPKAGQFMPKIADLRRQIEAANDGGHPGPEEAWSIAMGARGEAASVVWT
                     QQIAEAFFQAAMPLLDVGDKVAARLAFIERYERDVADARRAGARAMWFASLGTDPALR
                     VQAIDQALRMGRITAEQADGLLPRLPGAIDVPALEASAQESASPEDAHRRIAQLKTLL
                     RLP"
     gene            complement(1740..2525)
                     /locus_tag="PKN91_08950"
     CDS             complement(1740..2525)
                     /locus_tag="PKN91_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28241.1"
                     /translation="MARIRTIKPEFPQSESMGRVSRDARLAFVLMWTLADDAGRLRGN
                     SRMLASLLFPYDDDAPALIEGWIAELEHEGCVVRYKVDGQTYVEIRNWLIHQKIDKPS
                     QSKLPPFDESSRVLANPRESSSLDLRTKEGIKEGRGEEGKGGAEPSGPAPDAAPPPPE
                     SLPVITIPLVDKSEHPVMRSDVVEWSEAYPAVDVMQELRAMRQWCIAHPTRRKTARGV
                     RAFVVAWLGREQDRGGQRRPVGNRDAFAEFLGEPIDDGRTINA"
     gene            complement(2525..2989)
                     /locus_tag="PKN91_08955"
     CDS             complement(2525..2989)
                     /locus_tag="PKN91_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28242.1"
                     /translation="MMKQTAYLQKLRDPRWQRKRLEVMQRDRFACRMCGDSQSTLNVH
                     HVFYTRGADPWDYPDTALVTTCESCHEELHACDFGPRIVEALIAGGADLSALHGILTT
                     ISGTFNDGPHPVVLRPEQWIHAEGALFYALEAVANGASESDIRSALDRFKGA"
     gene            complement(2986..3333)
                     /locus_tag="PKN91_08960"
     CDS             complement(2986..3333)
                     /locus_tag="PKN91_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28243.1"
                     /translation="MTQLSMTFEAPPLADAQAMGRTGMQRAVDRAEREEPGFQERAYR
                     FLCWYAKLDDAPFPAEAVTELALSTGIEPPDGRAWGAVFQKAARAGFIRRSNVTYKRA
                     KGHGSLGVMWVAS"
     gene            complement(3330..3608)
                     /locus_tag="PKN91_08965"
     CDS             complement(3330..3608)
                     /locus_tag="PKN91_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28244.1"
                     /translation="MNAIQWTAEQICRLGKQTRPPVTSASRKALDVCRKLGRAITAFD
                     VAKAHEVANATASDWLKQARETGVLVCTGRRSHGNSRKLIYDIPKRRT"
     gene            complement(3642..3848)
                     /locus_tag="PKN91_08970"
     CDS             complement(3642..3848)
                     /locus_tag="PKN91_08970"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNS28245.1"
                     /translation="MQLTTEEILNALKDRGLTQVEIAKRTGIPQYRLSRWQNGEAPKT
                     ADDALRLRAYADTILNRKKRAQAA"
     gene            4025..4255
                     /locus_tag="PKN91_08975"
     CDS             4025..4255
                     /locus_tag="PKN91_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28246.1"
                     /translation="MGWIDERVGLADLPRPTAGNLRRALDEIELRDAADDRDLLMSVL
                     QRIMAADAVGTVTLTAELRRAANHVLVRVSLR"
     gene            4252..4473
                     /locus_tag="PKN91_08980"
     CDS             4252..4473
                     /locus_tag="PKN91_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28247.1"
                     /translation="MIEDAYCPVAWDRAQAAIADPEYVATALEEGVRPDTAKQLSMMF
                     HMLLVGRHDAADVHARKAVDLLAREYLEA"
     gene            4475..4651
                     /locus_tag="PKN91_08985"
     CDS             4475..4651
                     /locus_tag="PKN91_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28248.1"
                     /translation="MEREESPLMRSARKADLDWSITTSVLVLIGLMTVVFVFTFGLVT
                     LFDALVWLMEGSQS"
     gene            4729..5370
                     /locus_tag="PKN91_08990"
     CDS             4729..5370
                     /locus_tag="PKN91_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28249.1"
                     /translation="MAFAKKDDSVAVIKPANIVRTTLRVVGTAPLVINNFSFKARQKM
                     MADMATPKSAKKSKTERPPRDYQDDFMQARHISTDGWDGIACPAFRAAMIDACRTVGL
                     VMTKAKMSVFVIPDGFDKSDGTPLVKIEGGEPERTESLVRNDNGGADIRIRPMWRHWS
                     ANVTVEFDADMITPDSVVNLLDRAGRQVGVGEGRPFSKNSVGQGWGTFTVEQA"
     gene            5367..5924
                     /locus_tag="PKN91_08995"
     CDS             5367..5924
                     /locus_tag="PKN91_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28250.1"
                     /translation="MNTDDTIDPNDGDISSANVTDEVIRLVTEHDDRAAPRVLLEAAR
                     DPASPLHRYFTWDDGEAAERYRLAQAGTLYRKVRMKIIKLDAEAREIKFETIRAITSV
                     PIDRKKKDSESYGRTQTVMSDEQRRASVLRGIARELMALRNKYAKYSEFSDVWVVIDD
                     MADTFDPPSTKKVKPSGKEAPPPVA"
     gene            6285..6539
                     /locus_tag="PKN91_09000"
     CDS             6285..6539
                     /locus_tag="PKN91_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28251.1"
                     /translation="MNTQLRCKPGDLAVVVRTGPNMRCFLGKIIRVKEVVWFTSTPSW
                     ITDPPMLIDGKPLLFNDFCLRPVRGDELPACFVPRELEAA"
     gene            6688..7578
                     /locus_tag="PKN91_09005"
     CDS             6688..7578
                     /locus_tag="PKN91_09005"
                     /inference="COORDINATES: protein motif:HMM:NF024096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HNS28252.1"
                     /translation="MSTDLIVPEFAPGIYESMTNADYHAIEALSASGIKTMLQSPMHY
                     RFERENPREDTASMAMGSALHMAILEPDIYEANVIVIPEDAPARPTSRQLLAKKPSPA
                     TVEAIAWWADFDAKAQGKISLTAEQSKKVDGMAGSVRRHPFIKTMLCDGRAELSLLWR
                     DARLGIPCRCRFDYLRNDGIAIDLKSTVDASPLGFVRAVTTWKYHLQEAHYRNGYEHL
                     FNESLRAFIFVAVENVAPYGCAAYVLESNAVRFGIDRVEEAMLLYEQATKTGYWRGYT
                     EKIMPLQLPRYALQVAPINY"
     gene            7611..8129
                     /gene="bet"
                     /locus_tag="PKN91_09010"
                     /pseudo
     CDS             7611..8129
                     /gene="bet"
                     /locus_tag="PKN91_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414661.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
     gene            8363..8812
                     /locus_tag="PKN91_09015"
     CDS             8363..8812
                     /locus_tag="PKN91_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28253.1"
                     /translation="MTVQFRHSGRAEIKHINARKEGPDEDKSLAVDLKLQVRTGIEVL
                     DGFEPLLRGVLFLDSGAVRNEMMDAVRFKNRLEHYRIDMLGSSFFACRLGKFAVEPLD
                     GYEIRVHFTASFSPSGDEVARIAEYLLDEVELVIEPESGELDLDGEE"
     gene            8815..9033
                     /locus_tag="PKN91_09020"
     CDS             8815..9033
                     /locus_tag="PKN91_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28254.1"
                     /translation="MKRGAPRGYTQGYRRPSIRSAIQGGATTVVKIAESVGLGRSTVR
                     NHLYRMKRDGEVRQIRPWGERAAKWVLR"
     gene            9030..9347
                     /locus_tag="PKN91_09025"
     CDS             9030..9347
                     /locus_tag="PKN91_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28255.1"
                     /translation="MTRPPWTPEQLDTLRRLYAMTPTIQIAEQIGRPLRATYQKAKDL
                     GLSPDNAEQYRQAVMAAATLASIDAQTHPRGKPQSWRLMCEQTGRVVSYCAGEQGWTE
                     RAA"
     gene            9558..9851
                     /locus_tag="PKN91_09030"
     CDS             9558..9851
                     /locus_tag="PKN91_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28256.1"
                     /translation="MKKEQRQQRIHPAGMGGTQRLYRFPNEYGASVVQSPFSYGGGEG
                     LFELAVTAYFGEGMDDFELTYSTPITNDVIGYLTEEEVQSTLDQIRALPQERT"
BASE COUNT         1858 a         3184 c         3200 g         1627 t
ORIGIN      
        1 tgatccaccc acaggtcgac ttcctctcgg tcggcctgcg ggacgatgtg atcgagcacg
       61 ccctgaacgg cgcgctcgta gaactcctcg aaatcgtcct gctccatcgc gtcgaacgcg
      121 atcgaccgcg gcaccgcgac gagttcgcca gtctcgggat tcggcaagaa gtcgacgtgg
      181 ccggcggcga ccttcaccgc caccagggcc ttctcgcggt tgtcgtagac cgggtggcgg
      241 tcggccacga actcgaccag tgcgaagaac ttgcggtgat gggcgagatt gcgcatccgc
      301 ttgacctcgg cgcgcacggc ctggcccttc ggcatcctgg cgatccggtc gcgcgagtcg
      361 tcgtcggccg gcagtagcgc ggcgccagat cggacgagca tgatctcagc cacggatcgc
      421 ctcgacgttg aacgcgccct tgaccttgcg caccatgaac cacgaccaca tcgggaacat
      481 cctcgacgcg actagcaggc gcacgcgggc agcttctctg aggaacccct ttgtctccac
      541 catcgacagc gatccatcga gcgcgatgac gttgaagtcg accgtgaacc gcacgccgtc
      601 ggccaggcgc acggtgatgc cctcgtacat ccagtccttg atgagcccgg cgcgtgcctg
      661 cgcttctagc atctgcgcgt actcggcctc cagcttcgac ttgaacttcg gagcggccgg
      721 ctcgacggcc ggcggcggcg ccttgatgcg atccaggtgc tcggccagtt gatcgttcgt
      781 ccagcgggtc attcggcgcc caccactggc ctgcgaacgc gctcgagcat cgccttcacc
      841 gtcggcgtct gctcggtcgg acccagcggg atgccggcgc cgagccgctg ccggatgctg
      901 gtcgtgatcc gatcccactc ccttgatgcc accccgaagt gaaatcggca cgtccaccga
      961 tcgctaccgt gggtgctgtc ggaaagcgcg cccggcatgg ggcacccgta ggcgccgcag
     1021 ccggtgtatg cgggctctcc agcggtgccg ccgacgtccg ccgatgcctt ggcgtagcga
     1081 ctcatggcag cctcagtagg gtcttgagtt gcgcgatccg gcggtgcgca tcctccgggc
     1141 tcgccgactc ctgtgcgctg gcctccaagg cgggcacgtc gatcgcgccc ggcagccgcg
     1201 gcagtagccc gtcggcttgc tccgcagtga tccggcccat gcgaagcgcc tggtcgatgg
     1261 cctgcacccg caacgccggg tcggtgccca acgaggcgaa ccacatcgcc cttgcgccgg
     1321 cccgtctggc gtccgccacg tcgcgctcgt agcgttcgat gaatgcgagc cgcgccgcca
     1381 ccttgtcgcc cacgtcgagc aggggcatgg cggcctggaa gaacgcctcg gcgatctgct
     1441 gcgtccagac gaccgacgcc gcctcaccgc gcgcgcccat cgcgatcgac caggcttcct
     1501 ccgggcccgg gtgcccgcca tcgtttgcgg cctcgatctg ccggcgcagg tcggcaatct
     1561 tcggcatgaa ctgcccggcc ttcggatcct ggacgtgggc attgatcgcg ccgcgaacct
     1621 gctcaatcgc caggtcgcgc aggccgttcc agtagatcgc caccgtgtcc gccgatagct
     1681 tgcggtcgta gtagtcggcc acggcccgca gcgcctcggc gaaggcgcgc ttgtcgtcgt
     1741 cacgcattga tcgtcctccc gtcgtcgatc ggctcgccca ggaactccgc gaacgcatcg
     1801 cggttgccga cgggccgccg ctggccgcca cggtcctgtt ctcggcccag ccacgccacg
     1861 acgaaggcac gaacccctct tgcggtcttt cgccgggtcg ggtgggcgat gcaccactgc
     1921 cgcattgccc gaagctcctg catcacgtcg accgccgggt aggcctcgga ccactccacc
     1981 acgtcggatc gcatgaccgg gtgctccgac ttgtcgacca acgggatcgt gatgaccgga
     2041 agcgactcgg ggggaggcgg agcggcgtcg ggcgccgggc cgctcggctc ggcgcctccc
     2101 ttcccttcct ctcccctccc ttccttgatc ccttccttgg tccttagatc caacgacgag
     2161 gattcgcgag gattcgcgag gactcgcgag gattcgtcga acgggggtag tttcgactgg
     2221 ctcggcttgt cgatcttctg atgaatcaac cagttacgaa tctcgacgta ggtctgcccg
     2281 tcgaccttgt agcgaacgac gcacccctca tgctccaact cggctatcca cccctcgatc
     2341 agggccggcg catcgtcgtc gtagggaaaa agaaggctcg cgagcattcg cgaatttccg
     2401 cgaagcctcc cggcgtcgtc cgcaagagtc cacatcaaga cgaacgcgag gcgagcgtca
     2461 cggctcaccc ggcccatcga ctcggactgc gggaactccg gcttgatagt gcggatgcgt
     2521 gccatcacgc ccccttgaag cgatcaaggg cagagcgaat gtcagactcg ctcgcgccgt
     2581 ttgccaccgc ctcgagcgca taaaacaacg cgccctcggc atgaatccac tgctcggggc
     2641 gcaacacgac tggatgcggc ccatcgttaa aggttccgct gatggtcgtc agaatgccgt
     2701 gcagtgcgct caagtctgcg ccgccagcaa ttagcgcctc gacgattctc gggccgaagt
     2761 cgcaggcgtg aagttcttcg tggcacgact cgcacgttgt aacaagcgcg gtatcagggt
     2821 agtcccaggg gtctgcgccg cgcgtgtaaa agacatgatg aacattcagc gttgattggc
     2881 tatcgccgca catgcgacat gcgaaccgat cgcgttgcat tacttccaaa cgcttgcgct
     2941 gccaacgcgg atcgcggagc ttttgaagat aggccgtctg cttcatcacg aagccaccca
     3001 catcacaccg agcgacccgt gacccttggc ccgcttgtag gtgacgttcg acctgcggat
     3061 gaacccggcg cgcgcggcct tctggaacac ggcgccccag gcccgcccgt ccggcggctc
     3121 gatgccggtg gacagcgcga gctcggtcac ggcctctgcc gggaacggcg cgtcgtcgag
     3181 cttggcgtac cagcacagga atcggtaggc ccgctcctgg aagccgggct cctcgcgctc
     3241 ggcgcggtcg acggcccgtt gcattccggt gcggcccatc gcctgcgcgt cggcgagcgg
     3301 cggcgcctcg aaggtcatcg agagttgcgt catgtccgcc tcttgggaat gtcgtagatc
     3361 aacttgcgcg agttgccgtg gctgcgtcga cctgtgcaga ccagcacccc ggtttcccgc
     3421 gcctgcttga gccagtcgct cgccgtggcg ttggctacct cgtgagcctt ggcaacatcg
     3481 aacgcggtga tcgcgcggcc caacttccga caaacgtcaa gcgccttgcg agacgcactc
     3541 gtgaccggcg gccgcgtctg cttgccgagc ctgcagatct gctcggcggt ccactggatc
     3601 gcgttcatcg cggatccccc agtcccatgt cgatccccta cttaagcggc ttgcgcgcgc
     3661 ttttttcggt tcaggatcgt gtcggcgtag gcgcgaagtc gaagcgcatc gtcggcggtc
     3721 ttgggcgcct cgccgttctg ccagcgagaa agccggtact gcggaatgcc cgtgcgcttc
     3781 gcgatctcga cttgcgtcag accgcggtcc ttcagcgcgt taaggatttc ttcggtagtc
     3841 agttgcatgg ccctcgcccc agcgtttcac gcatcatatg tgacgaagca tatgtacgca
     3901 acccataccc cccgcttcgg cgggtttttc ttcgtccgcg cgaaaatata tgtgctgaag
     3961 cattgacaac ttccaatgtg ccgtcgcata ctcatcatca catcgcgacc aacaggagga
     4021 cgagatggga tggatcgatg aacgagtcgg ccttgccgac cttcccaggc ccacggccgg
     4081 gaacctgcgc cgggctctag acgagatcga gcttcgtgac gcggcggacg accgcgacct
     4141 gctcatgtcg gtgctgcaac gaatcatggc cgccgatgca gtcggcaccg tgacgctgac
     4201 cgccgaactg cgacgggccg cgaatcacgt cttggtgcga gtgagcctgc gatgatcgag
     4261 gacgcctact gcccggtggc ctgggatcgc gcccaggcgg ccatcgccga tccggagtac
     4321 gtcgccacgg ccctcgaaga gggcgtgcga ccggatacgg ccaagcaact ctcgatgatg
     4381 tttcacatgc tgctggtggg ccgccacgac gccgctgacg ttcacgcacg caaggccgtc
     4441 gacctgctgg cccgcgagta cctggaggca tgacatggaa cgcgaagaat cgcccctgat
     4501 gcgcagcgcg cgcaaggccg acctcgactg gtcaatcacc acaagcgtcc tcgttctgat
     4561 cggactgatg accgtggtct tcgtgttcac gttcggcctg gtcacgctgt tcgatgcact
     4621 ggtctggctt atggaaggat cgcaaagcta acaccgcagc gcacgcggca agtgcgacgg
     4681 ggctagatcc gctccccgaa cgccagcgga caacaccgga gaacggaaat ggcatttgcg
     4741 aagaaagacg acagcgttgc cgtcatcaag cccgccaaca tcgtgcgcac cactctgcga
     4801 gttgtgggga ccgcgccgct tgtgatcaac aacttcagct tcaaggctcg gcagaagatg
     4861 atggccgaca tggccacgcc gaagtcggcg aagaagtcca agaccgagcg gccgccgcgc
     4921 gactaccagg acgacttcat gcaggcgcgg cacatcagca ccgacggatg ggacggcatc
     4981 gcctgcccgg cgttccgcgc cgccatgatc gacgcctgtc gcaccgtcgg cctggtgatg
     5041 accaaagcga agatgagcgt gttcgtgatt cccgacggat tcgacaagag cgacgggacg
     5101 ccgctcgtga agatcgaggg cggcgagccc gagcgcaccg agtcgctggt gcgcaacgac
     5161 aacggcggcg cggacatccg tatccggccg atgtggcgcc attggtcggc gaacgtcact
     5221 gtcgagttcg acgccgacat gatcactccc gattcggtcg tgaacctact cgaccgtgcc
     5281 ggccgccagg tcggagtcgg cgagggccgg ccgttcagca agaacagcgt cggacagggc
     5341 tggggaactt tcaccgtgga gcaagcatga acacagacga caccatagat ccgaacgacg
     5401 gcgacatcag cagcgcgaac gtcaccgacg aggtaattcg cctggtgacc gagcatgacg
     5461 accgagccgc gccgcgcgtg ctgctggaag cggctcgcga tccggcgagc ccgctgcatc
     5521 gctacttcac ctgggacgac ggagaggcgg ccgaacgcta ccggctggcg caggccggca
     5581 ctctgtatcg caaagtgaga atgaagatca tcaagctcga cgccgaggcg cgcgagatca
     5641 agttcgagac gatccgcgcg atcaccagcg tcccgatcga ccggaagaaa aaagacagcg
     5701 agtcctacgg tcggacgcag accgtgatga gcgacgagca gcgtcgggcg agcgtgctgc
     5761 gcggaatcgc tcgcgaactg atggcgcttc gcaacaaata tgcgaaatac agcgagttca
     5821 gcgatgtgtg ggtcgtgatc gacgacatgg ccgatacgtt tgatccgccg agcaccaaga
     5881 aagtcaaacc ttccggtaaa gaagcgccgc ctccggtggc gtaacgcggc ggggcgtggc
     5941 cgacatgcgg ggcgtggcga ggcaggcacg gcccggcgag gcaggcgcgg cagggcgcgg
     6001 cagggcgagg cgaggccggg cctggcgagg caggcggggc cgggcgaggc gtggcagggc
     6061 gcggcaaggc aaggctaggc gtggcgtggc gcggcccggc gaggcaggcg cggcagggcg
     6121 cggcacggca aggcgaggcc gggcgaggcc ggcaggcatg gcatggcgag gacaggcgag
     6181 gactggccgg gcgtggcaag gcagggcgtg gcgaggcagg catggcgtgg cgtggcccgg
     6241 cctggcatgg cgaggcgagg cgaggcaggc aaatggagaa cgcgatgaac acacaactca
     6301 gatgcaaacc cggcgacctg gccgtcgtcg tgcgcaccgg acccaacatg cggtgcttcc
     6361 tcggcaagat cattcgggtc aaggaagtcg tctggttcac cagcacgcct tcatggatca
     6421 ccgatccgcc catgctgatc gacggcaagc cgctgctgtt caacgacttc tgcttgcggc
     6481 cggtccgcgg ggacgaactg ccggcctgct tcgtgccgcg cgaactggag gcagcatgag
     6541 tgaacacgat caaggccgcg ctgacggcaa ccgtgatctt cgtcgcgtgg ctatcgctcg
     6601 cttcgtccct agagccgcgc gaccgccggc accgcgactc gcgctcgcct gcactgcact
     6661 cgctttcatc atcctgtgga tcagcgcatg agcaccgacc tgatcgttcc agagttcgca
     6721 cccggcatct acgagtcgat gacgaacgct gactaccacg ccatcgaggc gctgtcggcc
     6781 agcggcatca agaccatgct ccagtcgccg atgcactacc gtttcgagcg cgaaaacccg
     6841 cgcgaggaca ccgcgtcgat ggcgatggga tcggctctgc acatggcgat cctcgaaccc
     6901 gacatctacg aggcgaacgt catcgtcatc ccagaggatg cgccggccag gccgaccagc
     6961 cggcagttgc tcgcgaagaa accgtcgccg gccaccgtcg aggcgatcgc ctggtgggcc
     7021 gacttcgacg caaaggcgca gggcaagatt tcgctcaccg cggagcagtc gaagaaggtc
     7081 gatggaatgg caggcagcgt gcggcgccat ccgttcatca agaccatgct ctgcgacgga
     7141 cgcgccgagc tctccctgct ctggcgggat gcgcgcctcg gaattccgtg ccgctgccgc
     7201 ttcgactacc tgcgcaacga cggcatcgcg atcgacctca agagcaccgt cgacgcgagc
     7261 ccgctagggt tcgtgcgcgc cgtgacgacg tggaagtacc accttcagga agcgcactac
     7321 cgaaacgggt acgagcacct gttcaacgag tcgcttcgcg cgttcatctt cgtggccgtc
     7381 gagaacgtcg cgccctacgg gtgcgcagcc tacgtcctcg aatcgaatgc ggtccgcttc
     7441 ggcattgatc gcgtcgagga agcgatgctg ctctacgagc aggccaccaa gaccggctac
     7501 tggcgcggat acaccgagaa gatcatgccg ctccagttgc cgcggtacgc gctccaggta
     7561 gcaccaatca actattgacc agtgaggaca gcatgaacgc agtcatggaa gtgaagaagc
     7621 cgcagagcct catcgagaag atggcggcga agtacagcgt cgacgcgagc aagatgctcg
     7681 gcacgctgaa ggccacggcg ttcaagggag aagtcagcaa cgagcagatg atggcgctga
     7741 tgatcgtggc cgatcagtac agcctgaatc cgtggacgaa ggaaatctac gccttcccgg
     7801 acaagaacaa tggcatcgtc ccggtcgttg gtgtcgacgg gtggtcgcgc atcatcaacg
     7861 accaccggga cttcgacgga atggagttca tcgagggtcc gctggacgac aagcagatcc
     7921 cgacgtggat cgagtgcgtg atcttccgga aagaccgcaa ccatgctgtg aaggtgcgcg
     7981 agtacctggc cgagtgccgc cgcgacacgc cgccgtggaa atcgcatccg cgccggatgc
     8041 tgcgccacaa ggcgatgatc cagtgcgcgc gcttggcgtt cggatacgcc ggcatctatg
     8101 accaggacga ggccgaacgg atcatcgacg ccgacgtgcg cgtggtgcag tcgaccgaag
     8161 tgatcgacga tctgaacgct cggatcgtgc ctgtcgacaa gaccgccggc atcaccttcg
     8221 ctcaagtcga gcagaagatg gtcgaggcaa aggacattga tctgctcgac gcatcggccg
     8281 accttatcgg tgaggttgca gacgctcgcc agcgtgacga actcacggcg ctctaccgtg
     8341 atctgcgagc caagctggag ggatgaccgt gcaattccgc cactcaggcc gcgcagagat
     8401 caagcacatc aacgcgcgca aggaaggccc ggacgaggac aagagcttgg ccgtcgacct
     8461 gaagctccag gttcgcacag gaatcgaagt actcgacggt ttcgagccgc tgcttcgagg
     8521 cgtgctgttc ctcgactcgg gcgcggtgcg caacgagatg atggacgcgg ttcggttcaa
     8581 gaaccggctg gagcactacc ggatcgacat gctcgggtcg tcattcttcg cgtgccggct
     8641 cgggaagttc gcggtggagc cgctggatgg atacgagatc cgcgttcact tcacagcctc
     8701 gttctcgccg agcggtgacg aggttgcgcg gatcgcagag tacctgctcg acgaagttga
     8761 actggtgatc gagccggaga gcggcgaact cgacctggat ggagaggagt gactgtgaaa
     8821 cgaggggcgc cgcgaggcta cacgcagggc taccgccgtc cctcgatccg atccgcgatc
     8881 cagggcggcg cgaccacggt cgtcaagatc gcagaatccg tcgggctcgg ccggtcgacc
     8941 gtgcgcaatc acctgtaccg gatgaagcga gacggcgagg ttcgccagat caggccgtgg
     9001 ggcgagcgcg cggcgaagtg ggtgctgcga tgacccgccc cccctggacc cccgagcaac
     9061 tcgacacgct gcgccggctc tacgccatga cgccgacgat tcagatcgcc gagcagattg
     9121 gccgcccact acgcgcgacc taccagaagg cgaaggacct tggcctgagc ccggacaacg
     9181 ccgagcagta ccgacaggct gtgatggccg ccgccacgct ggcatcgatc gacgcacaga
     9241 cgcacccgcg gggcaagccg cagtcgtggc ggttgatgtg cgagcagacg ggccgggtgg
     9301 tgagctactg cgccggagag cagggatgga cggagcgggc ggcatgaata tcgtgaccga
     9361 gaagcgcaaa tcgcaggccc gcaagcacgc cagccggacg aggacatgcg tctgcggaaa
     9421 ggtctgtcgc gggaacggcg ggtggagcag ccacaagcga gcctgcgccc tttggcggga
     9481 ggcggtcttg tccgcccgtg gaggtatcga gcgatgaaca tcttcaccgc cccccgcatc
     9541 tatccgggag agaggacatg aaaaaggaac agcgacagca acgcattcat ccagccggca
     9601 tgggaggaac acagcgtctt tataggtttc ccaacgagta tggcgcgagt gtcgtccagt
     9661 ctccgttttc ttatggcggc ggcgaggggc tttttgaact tgcggtgacg gcgtacttcg
     9721 gcgaaggcat ggatgacttt gaactcacat actccacgcc aataaccaac gatgtgattg
     9781 gttatctgac agaggaagaa gttcaatcga ccctcgatca gatcagggcg ttgccgcagg
     9841 agaggacatg aaacgcaccg acctgatcg
//
LOCUS       DAOQVY010000091         9835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_7727_length_9835_cov_5.762577, whole genome shotgun sequence.
ACCESSION   DAOQVY010000091 DAOQVY010000000
VERSION     DAOQVY010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9835
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7727_length_9835_cov_5.762577"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1835)
                     /locus_tag="PKN91_09035"
     CDS             complement(<1..1835)
                     /locus_tag="PKN91_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNS28257.1"
                     /translation="MTTHDDQSLIRTTSSLGRYVTVAVALALLAVASFWFLGRTSSPA
                     GVAATALQSIALDAGVAARGDAAALRAVADNLAELASLAKRSPQSSWVRDRRWATLQS
                     DGAALAQATPALTAAAAAADAVGNSAPAMATEMAAMQTAVPGAAASSAIGRPLDLYAT
                     ALARIELDVGRLATGSGAQAEIAARLAENTDYVGRVIKGLNGADASLGLPRAAGADAT
                     RRAAALGEEFEALNTAVGTVVNAASTLAAGKATAERMASSARALANLAVTGAGSSGPA
                     WLAWVPVGLGLAAILPLLLLLQKFVALRKGVERQRALVDTQSRDNDRNQQAILRLLDE
                     LSSLADGDLTVQATVTEDITGAIADSINYAIEALRGLVTTINQSSVQLDSAARQTQAL
                     SQHLAKASVAQSKQVASASESAGAMAANTEEISGNAERASDVARHSVDVAHKGGEAVR
                     RTIDGMNAIRETIQETSKRIKRLGESSQEIGSIVELINDIAEQTNILALNASIQASMA
                     GDAGRGFAVVADEVQRLAERAALATKQIEVLVRTIQTDTNEAVVSMERSTTDVVGGAL
                     LAENAGAALEEIEQVSNQIASLVQNISSSSKEQSSVAGAITKNMHV"
     gene            complement(1848..2411)
                     /locus_tag="PKN91_09040"
     CDS             complement(1848..2411)
                     /locus_tag="PKN91_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNS28258.1"
                     /translation="MSEAVPLRSLRDRPYELLSELDRRGRAVSAQVAQEGSAGREWVG
                     VALRMAGELFLVAREETREVLGVPGSLTRVPGAKSWVRGLANVRGQLLPVLDLRQFLG
                     SGVTPVTRNARVVVAHHREIPAGLLVDEVLGFRRFAEAEFSGDVPPTIVRCERYLAGT
                     FRRGQEQWPVLSLRTLIESQAFLEAAA"
     gene            complement(2413..2778)
                     /locus_tag="PKN91_09045"
     CDS             complement(2413..2778)
                     /locus_tag="PKN91_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS28259.1"
                     /translation="MALILIVDDSPTEVHVFKNALERHGFKTATATDGAEGVRMAREI
                     HPDLIFMDIVMPNMNGYQATRALSNDPHTRAIPIVMVTSKGQETDKIWGLRQGAVDYM
                     VKPVSPDQLVAKAQATLAA"
     gene            complement(2784..3176)
                     /gene="pilG"
                     /locus_tag="PKN91_09050"
     CDS             complement(2784..3176)
                     /gene="pilG"
                     /locus_tag="PKN91_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HNS28260.1"
                     /translation="MNGANSKLGGLKVLVIDDSKTIRRTAETLLTKEGCEVFTAIDGF
                     DALAKIADHQPDIVFVDIMMPRLDGYQTCSLIKHNKVYKSIPVIMLSSKDGLFDRARG
                     RIVGSEHYLTKPFTKDELLAAIESHVGR"
     gene            3311..4264
                     /gene="gshB"
                     /locus_tag="PKN91_09055"
     CDS             3311..4264
                     /gene="gshB"
                     /locus_tag="PKN91_09055"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003195061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HNS28261.1"
                     /translation="MTLRRRLAVVMDPIADIKYAKDSTLAMLLAAGARNWDLWYLEQH
                     DLSLRDGVAWGRARSLAVRADPVSWFTLGERQDLRLGELNCILMRKDPPFDTEYIYTS
                     YILERAELAGALVVNRPRGLRDMNEKVYTAWFPQCCAPTLITRDMGEMTGFLASQGKI
                     VAKPLHGMGGRSIFVLEAGDKNANVVFETLTDYGQRFAIVQRYLPEIAATGDSRVLLV
                     DGEPIPYALARIPGATDHRGNLAAGAKGRGRPLNDRDRWLAAEIGPKLAADGMLFVGL
                     DVIGGFVTEINVTSPTGIRELDQQFGLNIGGVLIDAIERRL"
     gene            4272..5150
                     /locus_tag="PKN91_09060"
     CDS             4272..5150
                     /locus_tag="PKN91_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNS28262.1"
                     /translation="MASAALAVKEGIAPVRDRLVTMGFFAALLHGIVILGVTFGAPGG
                     NSAAPGLQVLLVADEVPAADRNDDASYLAQRTQLGSGAGAAVRTPRSQARMGSAQPPE
                     PVDVTGADANERVLTANVQRSTMTWIGTLDTPREERRPPALPDVALPDAIAGEDLETS
                     ELAGKERKELWITPDTRESSLAPYLDAWRRKVERLGTLNFPAAARQAPGAASPVLEVA
                     IDSNGQLASAVIQRSSGSPELDAAAISILRLASPFEPFPRELAANYRVLRFAYAWEFA
                     ASGTTSGTVTAPADSR"
     gene            5184..5747
                     /locus_tag="PKN91_09065"
     CDS             5184..5747
                     /locus_tag="PKN91_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNS28263.1"
                     /translation="MGASTLTNQLLVAMPSLADPNFAQTVTLICEHCVDRGALGIVVN
                     KPLPMKLADVLSQMKLDPLDESIGEQPVLRGGPVHTDRGFVLHRPGGHWDSSHQVSEL
                     IQVTTSRDVLAAMANGQGPGDAFIALGYAGWESGQLEREMLDNAWLSIPADENIVFEL
                     PYEERWPAVWRRLGVDLEKMTPFAGHA"
     gene            5731..6177
                     /gene="ruvX"
                     /locus_tag="PKN91_09070"
     CDS             5731..6177
                     /gene="ruvX"
                     /locus_tag="PKN91_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNS28264.1"
                     /translation="MPGTPDAVRVALAFDFGLRRIGVAVGDTLTRSAAARATVPAHGS
                     GPDWPAIDRLVRDSAPHVLVVGKPYNAAGDDSEMTTLAGQFAAELAARYGLPVEQVDE
                     RYSSIEATSHLKAQRASGERPRRLRKEDVDSAAAVVILRRWLEGER"
     gene            6174..7121
                     /locus_tag="PKN91_09075"
     CDS             6174..7121
                     /locus_tag="PKN91_09075"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNS28265.1"
                     /translation="MTRQTRADGSLRHLLSLESLSRGEIERLLDRAQQYVRPLGARPA
                     TSDELAGITVANLFTEPSTRTRVSFELAAKRLGAEVVNLEVQLSSRAKGESMLDTIFT
                     LESLHVDVFVIRDAEAGVTRLVASSVAPHVSVLSAGEGSVSHPTQGLLDALTIRQRKG
                     RIDGLTVAIVGDVRHSRVARSAYQALTTLGAAEVRIVAPPALLPADDEFGGCSRHTDI
                     EAGLRGADVVMALRIQRERMQDEAQLDAAAYFSEFGLTSARLRLARPDAIVMHPQPMN
                     RGVEIAPDVADGPNSAIRDQVRNGVAVRMAVLATVVRRA"
     gene            7118..8017
                     /locus_tag="PKN91_09080"
     CDS             7118..8017
                     /locus_tag="PKN91_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNS28266.1"
                     /translation="MSAPLTVGKAHRGTICLEDARLLARQSFSGDQYILRLEAPRCAA
                     RATPGSFVHISCDPDIPMRRPLSIMRADPHKGWIDLLFKVVGDGLAALAERKVGETLS
                     MLGPIGHGFEPHPARPLTLLIGGGVGIPPMIFLAEQLAGRDDAQWQPLVLMGSEIPFP
                     FDTRPSTIMVPGIPHGVIAAMPALDAMGVASRLASKAGFPGCYDGWVTQLADDWLHTL
                     ARERRAEIEIFACGPTLMLEATARLARKYELPCQVSLEEFMACAVGGCAGCAVRVDTD
                     DGPAMKRVCVDGPVFDAYSVFAG"
     gene            complement(8032..9168)
                     /locus_tag="PKN91_09085"
     CDS             complement(8032..9168)
                     /locus_tag="PKN91_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNS28267.1"
                     /translation="MKATTKAVGSTGDTTTQAPAVPEGPLAINTRPLFKLMVEKKASD
                     LFFTSNAPIKIKIEGQIFPVNKQILTPETVRQSAYALMTPEQLEYFHRELEIDFAISE
                     PGLGRFRVNVFHQRGYPAMVMRYITADMPKLDTLGLPEVFRELIMLKRGLILLVGATG
                     SGKSTTLASMINYRNEHSSDHIVTIEDPIEFLHTNKRSIVNQREVGLDTKSYERALRS
                     VMRAAPDVILVGEIRDRETMQACINLSGTGHLVLATLHANNCAETMDRIINMFPRDQH
                     NQIFLDLSQYLKAIAAQRLVFGKDGKRLAAVEVMLNTPHISELVKKGDVSGIKEAIVQ
                     SSERGVQSFDTALYELVRQGRIELEEALNNADSRTNLEAKINFG"
     gene            complement(9165..>9835)
                     /locus_tag="PKN91_09090"
     CDS             complement(9165..>9835)
                     /locus_tag="PKN91_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNS28268.1"
                     /translation="TIEDPVEYVHQHKGCVITHREIGVDTDSWHAALKNTLRQAPDVI
                     LIGEIRDRETMEYGIQFAETGHLVLATLHANSANQALDRIINFFPDERREQLLMDLSL
                     NLRGLISQRLIPRESGSGRIAAMEIMLNSPLIQDLIFKGEVAKIKEVMSRSTRIGMKT
                     FDQALFELYEGGYISYEDALRNADSKNELRLKIKLESKRENKNIDDGAGALRIVEEEQ
                     GRVI"
BASE COUNT         1484 a         3343 c         3346 g         1662 t
ORIGIN      
        1 acgtgcatgt tcttcgtgat tgcgccggcc acgctcgatt gctccttcga ggagctcgag
       61 atgttctgca cgaggctcgc gatctggttc gacacctgct cgatctcctc gagcgcggcg
      121 ccggcattct cggcgagcag cgcgccgccg accacatcgg tcgtgctgcg ctccatcgag
      181 accacggctt cgttggtgtc ggtctggatc gtgcgcacca gcacttcgat ctgcttggtg
      241 gcgagcgcgg cgcgctcggc gaggcgctgc acctcgtcgg cgacgaccgc gaagccgcgg
      301 ccggcatccc cggccatcga cgcctggatc gaggcgttca gcgcgaggat gttggtctgc
      361 tcggcgatgt cgttgatcag ctcgacgatg ctgccgatct cctgcgagct ctcgcccagg
      421 cgcttgatgc gcttgctcgt ctcctggatc gtctcgcgga tcgcgttcat gccgtcgatc
      481 gtgcgccgca cggcttcgcc gcccttgtgc gccacatcga ccgagtggcg cgcgacgtcg
      541 gatgcgcgct cggcgttgcc ggagatctcc tcggtattcg cggccatggc accggcggac
      601 tcggatgccg aggccacctg cttcgactgc gcgacgctcg ccttggccag gtgctgcgaa
      661 agcgcctggg tctggcgcgc cgcactgtcg agctggacgg aggactggtt gatcgtggtc
      721 acgaggccgc gcaatgcctc gatcgcgtaa ttgatcgagt cggcgatggc gccggtgatg
      781 tcttcggtga cggtcgcctg tacggtgagg tcgccatcgg cgaggctcga gagttcatcg
      841 agcagacgca ggatcgcctg ctggttgcga tcgttgtccc gggactgggt atcgacgagt
      901 gcgcgctggc gctccacgcc cttgcgcagg gcgacgaact tctgcagcag cagcagcagc
      961 ggcaggatcg cggccaggcc cagccccacc ggcacccagg cgagccacgc gggcccgctg
     1021 ctgcctgcgc cggtaaccgc aagattggcg agcgcgcgcg cactgctcgc catgcgttcc
     1081 gcggtggcct tgccggccgc gagtgtcgag gcggcattga cgaccgtgcc cacggccgta
     1141 ttcagcgcct cgaactcttc gccgagcgcc gcggcgcggc gcgtggcgtc agcacctgcg
     1201 gcgcgtggca ggccgaggct cgcatcggca ccgttcagcc ctttgatgac ccgaccgacg
     1261 taatcggtgt tctcggccag gcgcgcggcg atctcggcct gcgcaccgct gcccgtggcg
     1321 aggcggccga catcgagttc gatgcgcgcg agcgcggtcg cgtacagatc gagcggccgg
     1381 ccgattgcgg aacttgccgc cgcacccggc acggcggtct gcatggccgc catttcggtg
     1441 gccatcgcgg gcgcggagtt gcccaccgcg tccgcggccg ccgccgccgc cgtgagtgcc
     1501 ggggtcgcct gcgcgagcgc ggcgccatcc gactgcaacg tggcccagcg ccggtcacgt
     1561 acccaggagg actgcggcga gcgcttcgcg agcgacgcca gctcggcgag attgtcggcc
     1621 acggcgcgca gcgccgcggc atcacctcgc gcggccacgc ccgcatcgag cgcgatgctc
     1681 tgcagcgcgg tggcggcgac gcccgcaggt gaactggtgc gccccaggaa ccagaagctg
     1741 gcaacggcca gcagcgcaag cgccaccgcg acggtcacat agcggccgag gctgctggtg
     1801 gtgcggatca gtgactggtc gtcgtgcgtg gtcattgtcg tttcttgtca ggccgctgct
     1861 tcgagaaacg cctggctctc gatcagagtg cgcaggctga ggaccggcca ttgctcctgg
     1921 ccgcgcctga aggtgccggc gagatagcgc tcgcagcgca cgatcgtcgg cggcacgtcc
     1981 ccggagaact ccgcttcggc gaagcgccgg aagccaagca cttcatcaac cagcagcccc
     2041 gcgggtatct cgcggtggtg cgcgacgacg acgcgcgcgt tgcgggtcac cggcgtcacg
     2101 ccgctgccga ggaactggcg caggtcgagc accggcagca gctgtccgcg cacgttggcg
     2161 aggcccctga cccagctctt cgcgcccggc acgcgggtca gcgagcccgg cacgccgagc
     2221 acctcgcgag tttcctcgcg ggcgaccagg aacagctcgc cggccatgcg cagcgccacc
     2281 ccgacccact cgcggccggc cgagccctcc tgcgccacct gggccgatac cgcccgcccg
     2341 cgccggtcga gttccgaaag caactcgtag ggccggtcgc gcaggctcct gagtgggacg
     2401 gcttcgctca tgtcaggcgg caagagtcgc ctgcgccttg gcaacgagct gatcgggcga
     2461 cacaggcttg accatgtagt cgaccgcgcc ctgccgcagc ccccagatct tgtcggtttc
     2521 ctggcccttc gaggtcacca tgacgatcgg gatcgcgcgg gtgtgcgggt cgttcgacag
     2581 cgcacgggtc gcctggtagc cgttcatgtt gggcatcacg atgtccatga agatcagatc
     2641 ggggtggatc tccctggcca tgcgcacgcc ctcggcgccg tcggtggcgg tcgcggtctt
     2701 gaaaccgtgc cgctcgagcg cgttcttgaa gacgtgcact tcagtgggtg aatcgtcgac
     2761 gatcagtatg agcgccatcg ccatcatctc ccgacatgcg actcgatcgc cgcaagcagc
     2821 tcgtccttcg tgaaaggctt ggtgagatag tgctcggagc cgacgatgcg gccccgggcg
     2881 cggtcgaaca ggccatcctt cgaggacagc atgatcaccg gaatggattt gtagaccttg
     2941 ttgtgcttga tcagcgagca ggtctggtag ccgtcgagcc gcggcatcat gatgtcgacg
     3001 aacacgatgt cgggctggtg gtcggcgatc ttggcgagcg catcgaaccc gtcgatggcg
     3061 gtgaagacct cgcaaccttc cttcgtcagc agcgtctcgg ccgtgcgacg gatggtcttg
     3121 ctgtcatcga tgacgaggac cttgagaccc ccgagcttcg agttggcgcc attcacttga
     3181 acactactcc ccgcgcgttt tcgcgcagaa tcgacaagca agctgccgcg cgttttaaca
     3241 tatcggtata ccccctgcca tgccggccgc gtcacgtttc cggcaaacgg cgggacccga
     3301 ggagccctgg atgaccctac gccgacgact ggccgtggtg atggacccca tcgccgacat
     3361 caagtacgcc aaagactcca ccctggccat gctgctggcc gccggggccc gcaactggga
     3421 cctgtggtac ctggagcagc atgatctctc actgcgggac ggcgtcgcgt ggggccgcgc
     3481 acggtcgctc gcagtgcgtg ccgatcctgt gagctggttc acactcggcg agcggcagga
     3541 cctgcgcctc ggcgagctca actgcatcct gatgcgcaag gacccccctt tcgacaccga
     3601 atacatctac acctcgtaca tcctcgagcg ggccgaactc gccggcgcgc tggttgtcaa
     3661 ccggccccgc ggcctgcgcg acatgaacga gaaggtgtac acggcgtggt tcccgcagtg
     3721 ctgtgcgcca acgctcatta cgcgcgacat gggcgagatg acgggcttcc tcgccagcca
     3781 gggaaagatc gtcgccaaac ccctgcacgg catgggcggg cgctcgatct ttgtgctcga
     3841 ggccggcgac aagaacgcga acgtcgtctt cgagacgctc accgactatg gccagcggtt
     3901 cgcgatcgtc cagcgctacc tgcccgaaat cgccgcaacg ggcgactcgc gcgtgctgct
     3961 ggtcgacggc gagccgatcc cctacgcgct cgcgcgcatt ccgggcgcca ccgaccatcg
     4021 cggcaacctc gccgccgggg cgaagggccg ggggcggcca ctcaacgacc gcgaccgctg
     4081 gctcgccgcc gagatcggcc cgaagcttgc tgccgacggc atgctgttcg tcggcctcga
     4141 tgtgatcggc ggcttcgtca ccgaaatcaa cgtgacgagc cccaccggca tccgcgagct
     4201 cgaccagcag ttcggcctga acatcggcgg agttctcatc gacgcgatcg agcggcgtct
     4261 ataatttcca cgtggcgtcg gcagccctgg cagtgaagga aggtatcgcc ccggtccggg
     4321 accggctggt gacgatgggc tttttcgccg cgctgctgca cggcatcgtg atcctcgggg
     4381 tgaccttcgg cgcgccgggt ggcaatagcg cggcgcctgg cctgcaggtg ctgctcgtcg
     4441 ccgacgaagt gcccgcggct gaccgcaacg acgacgcgag ctacctcgcg cagcgcaccc
     4501 agctcggttc cggggcggga gccgccgtgc gcacaccgcg cagtcaggcc cgtatgggca
     4561 gcgcgcagcc gcccgagccc gtcgatgtga ccggcgcgga cgcgaacgag cgcgtactga
     4621 ccgccaacgt gcagcgcagc acgatgacct ggatcggtac cctcgacacg ccgcgcgagg
     4681 agcggcggcc gcccgccctg cccgacgtcg cgctgcccga cgccatcgcc ggcgaagatc
     4741 tcgagacgag cgagctcgcg ggcaaggaac gcaaggagct gtggatcacg ccggacacgc
     4801 gtgagtcgag cctcgcgccg tatctcgatg cgtggcggcg caaggtcgag cgcctcggca
     4861 cgctcaattt cccggccgcg gcgcgtcagg cgccgggcgc ggcgagtccg gtgctcgagg
     4921 tcgcgatcga ctcgaacggc caactcgcct cggcggtgat ccagcgctcg agcggctcgc
     4981 ccgagctcga cgcggcggcg atttcgatcc tgcgcctggc gagcccgttc gagccattcc
     5041 cgcgcgaact cgcggcgaac tatcgcgtgt tgcgtttcgc ttacgcctgg gagttcgccg
     5101 cgagcggcac gacgagtgga accgtgacgg cacctgccga ttcccgctag cgcgacgccg
     5161 cgcgagtgcc catacttgac tgcatgggcg catcgacact caccaatcag ctgctggtcg
     5221 cgatgccctc gctggcggac ccgaacttcg cacagaccgt cacgctgatc tgtgagcact
     5281 gcgtcgaccg cggcgcactc ggcatcgtcg tcaacaagcc gctgccgatg aagctcgccg
     5341 acgtgctctc gcagatgaag ctcgatccgc tcgatgaaag catcggcgag cagccggtac
     5401 tgcgcggcgg tccggtgcac accgaccggg gcttcgtgct gcaccggccg ggcggccact
     5461 gggactcgag ccaccaggtc tccgaactga tccaggtcac cacctcgcgc gacgtgctcg
     5521 ccgcgatggc aaacggccag ggcccgggcg acgcgttcat cgcgctcggc tacgccggct
     5581 gggaatcggg ccagctcgag cgcgagatgc tcgacaacgc gtggctgtcg atcccggccg
     5641 acgagaacat cgtgttcgaa ctgccctacg aggagcgctg gcccgcggtg tggcggcggc
     5701 tcggcgtcga cctcgagaag atgaccccct ttgccgggca cgcctgacgc ggtacgcgtc
     5761 gcactcgcct tcgatttcgg cctgcggcgc atcggcgtcg cggtcggcga cacgctgacc
     5821 cgcagcgccg ccgcgcgcgc caccgtgccc gcgcatggct cgggccccga ctggccggcg
     5881 atcgatcgcc tggttcgcga cagcgcgccg cacgtactcg tggtcggcaa gccttataat
     5941 gccgctggtg acgacagcga aatgaccacg ctcgcggggc agttcgccgc ggagcttgcg
     6001 gcgcgctacg gactgcccgt cgaacaggtc gacgagcgtt actcctccat cgaggccact
     6061 tcccacctga aggcgcagcg cgccagcggt gagcgtccgc ggcggctgcg caaggaggac
     6121 gtggacagcg cggcggcggt ggtgattctc aggcgatggc tggaagggga acgatgacga
     6181 gacagacgcg tgcggatggc tcactgcgcc acctgctgtc actcgagagc ctgtcgcgcg
     6241 gggaaatcga gcggctgctc gatcgcgcgc agcagtatgt ccgcccgctc ggtgcacgtc
     6301 ccgcgaccag cgacgaactc gcgggcatca cggttgccaa cctcttcacc gagccctcga
     6361 cccgcacgcg cgtttcgttc gagctcgccg ccaaacgcct cggcgccgag gtcgtgaacc
     6421 tcgaggtgca actgtcctcg cgcgccaagg gcgagagcat gctcgacacg attttcacgc
     6481 tcgaatcgct gcacgtcgac gtattcgtga tccgggacgc cgaggccggg gtgacgcgcc
     6541 tcgtcgcctc gagcgtcgcg ccccatgtca gcgtgctgtc ggcgggcgaa ggcagcgtct
     6601 cgcaccccac ccagggcctg ctcgatgcgc tgaccatccg ccagaggaag ggccgcatcg
     6661 acggtctcac cgtcgcgatc gtcggcgacg tgcgccactc gcgcgtcgcg cgctcggcgt
     6721 accaggcgct gacgacgctc ggtgccgccg aggtgcgcat cgtcgcgccg cccgcgctgc
     6781 tgcccgccga cgacgaattc ggcggctgca gccgccacac cgacatcgaa gcgggactgc
     6841 gcggtgccga cgtcgtgatg gcgctgcgca tccagcgcga gcggatgcag gatgaggcgc
     6901 agctcgacgc cgcggcctat ttcagcgaat tcggcctgac gagcgcgcgc ctgcgcctcg
     6961 cacggcccga tgcgatcgtg atgcacccgc agccgatgaa ccgcggcgtc gagatcgctc
     7021 ccgacgtcgc cgacggaccg aactcggcca tccgcgacca ggtgcgcaac ggcgtcgcgg
     7081 tgcgcatggc ggtgctcgcc accgtggtgc gcagggcatg agcgcgccgc tcacggtggg
     7141 caaggcgcat cgcggcacga tctgcctcga ggacgcgcgg ctgctcgcgc gccagtcctt
     7201 cagcggcgat cagtacatcc tgcggctcga agcgccgaga tgcgccgcgc gcgccacccc
     7261 gggctcgttc gtgcacatca gctgcgatcc cgacataccg atgcgccggc cgctgtcgat
     7321 catgcgtgcc gacccgcaca agggctggat cgacctgctg ttcaaggtgg tcggcgatgg
     7381 gctcgccgcg ctcgccgagc gcaaggtggg cgagacgctc tcgatgctcg gcccgatcgg
     7441 ccacggcttc gagccacacc cggcccgccc gcttactctt cttatcggcg gcggcgtcgg
     7501 catcccgccg atgatattcc tcgccgagca actcgccggg cgcgacgatg cgcagtggca
     7561 gcccctcgtg ctcatgggct ccgagattcc cttcccgttc gacacgcgcc cctcgacgat
     7621 catggtgccc gggatcccgc acggcgtgat cgccgcgatg cccgctctag atgccatggg
     7681 tgttgcgagc cggctcgcga gcaaggcggg tttcccgggc tgttacgacg gctgggtcac
     7741 gcagcttgcc gacgattggc tgcatacgct ggcgcgcgag cggcgtgccg aaatcgagat
     7801 cttcgcctgc ggcccgacct tgatgctcga ggcgaccgcg cgcctcgcgc gcaagtacga
     7861 actgccctgc caggtctcgc tcgaggaatt catggcctgc gcggtcggcg gctgcgccgg
     7921 ttgcgcggtg cgtgtggaca ccgatgacgg accggcgatg aagcgcgtgt gcgtggatgg
     7981 gccggtgttc gacgcgtaca gcgtattcgc gggctgactg acgtcccgct ctcagccgaa
     8041 gttgatcttc gcctcgagat tggtgcgcga gtcggcgttg ttgagtgctt cctcgagctc
     8101 gatgcgcccc tgcctgacga gctcgtagag cgcggtgtcg aagctctgta cgccgcgctc
     8161 gctgctctgc acgatcgcct ccttgatgcc cgacacgtcg cccttcttga cgagctcgga
     8221 gatgtgcggc gtgttgagca tcacttcgac ggcggcgagg cgcttgccgt ccttgccgaa
     8281 caccagccgc tgggcagcga tcgccttcag gtactgcgag aggtcgagga agatctggtt
     8341 gtgctggtcg cgcgggaaca tgttgatgat gcggtccatc gtctcggcgc agttgttggc
     8401 gtgcagggtc gcaagcacca ggtggccggt gccggagagg ttgatgcagg cctgcatcgt
     8461 ctcgcggtcg cggatctcgc cgaccaggat cacgtcgggc gcagcgcgca tcacgctgcg
     8521 cagggcgcgc tcgtagctct tggtgtcgag cccgacttcg cgctggttga cgatcgagcg
     8581 cttgttggtg tgcaggaact cgatcgggtc ctcgatcgtg acgatatggt ccgaggaatg
     8641 ctcattgcgg tagttgatca tcgacgcgag cgtcgtcgac ttgcccgagc cggtggcgcc
     8701 gaccagcagg atcaggccgc gcttgagcat gatcagctca cggaagacct ccggcaggcc
     8761 gagcgtgtcg agcttcggca tgtcggcggt gatgtagcgc atgaccatcg cgggatagcc
     8821 gcgctggtgg aagacgttga cgcgaaaacg cccgaggccc ggctcggaaa tcgcgaagtc
     8881 gatctcgagc tcgcggtgga agtattcgag ctgctcgggg gtcatcagcg cgtaggccga
     8941 ctggcgcacg gtctcggggg tcaggatctg cttgttgacc gggaagatct ggccctcgat
     9001 cttgatcttg atcggcgcat tggaggtgaa gaagaggtcg gaggccttct tctccaccat
     9061 cagcttgaac agcggccgcg tgttgatcgc gagcgggcct tcgggcaccg cgggcgcctg
     9121 ggtcgtcgtg tcgccggtcg agccgacggc cttggtcgtc gccttcatat cacgcgtccc
     9181 tgctcttcct cgacgatgcg cagcgcaccg gcgccgtcgt cgatgttctt gttctcgcgc
     9241 ttgctctcga gcttgatctt caggcgcagt tcgttcttcg agtcggcgtt gcgcagcgcg
     9301 tcctcgtagg agatgtagcc gccctcgtac agctcgaaca gcgcctggtc gaaggtcttc
     9361 atgccgatgc gggtcgagcg cgacatgact tccttgatct tggcgacctc gcccttgaag
     9421 atcaggtcct ggatcagcgg cgagttcagc atgatctcca tcgccgcgat gcgcccactg
     9481 cccgattcgc gcggaatcag gcgctgcgag atcaggccgc gcaggttcag cgacaggtcc
     9541 atcagcagct gttcgcgacg ctcgtcggga aagaaattga tgatgcggtc gagcgcctgg
     9601 ttggcgctgt tggcgtgcaa ggtcgcgagc acgaggtggc cggtctcggc gaactggatg
     9661 ccgtactcca tcgtctcgcg gtcgcggatc tcgccgatca ggatcacgtc gggcgcctgc
     9721 cgcagcgtgt tcttcagcgc ggcgtgccac gaatcggtgt cgacgccgat ctcgcggtgc
     9781 gtgatcacgc agcccttgtg ctggtgcacg tactcgaccg ggtcctcgat cgtga
//
LOCUS       DAOQVY010000092         9671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_7874_length_9671_cov_5.140495, whole genome shotgun sequence.
ACCESSION   DAOQVY010000092 DAOQVY010000000
VERSION     DAOQVY010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9671
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7874_length_9671_cov_5.140495"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /gene="carB"
                     /locus_tag="PKN91_09095"
     CDS             complement(<1..1074)
                     /gene="carB"
                     /locus_tag="PKN91_09095"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNS28269.1"
                     /translation="MPKRTDIQSILIIGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPINWRTIARIIAKERPDALLPTMGGQTALNTALDL
                     DRQGVLEEYGVELIAASRAAIDMAEDRERFRNAMREIGLESPRSHIAKSLEDALAVVA
                     EIGYPCVIRPSFTLGGSGGGIAYNREELEAIVTRGLEASPTSEVLIEESVIGWKEYEM
                     EVVRDRKDNCIIICSIENLDPMGVHTGDSITVAPAQTLTDKEYQRMRDASIAVLRKIG
                     VDTGGSNVQFSVNPADGRMLIIEMNPRVSRSSALASKATGFPIAKIAAKLAIGYTLDE
                     LQNDITGGATPASFEPAIDYVVTK"
     gene            complement(1079..2239)
                     /gene="carA"
                     /locus_tag="PKN91_09100"
     CDS             complement(1079..2239)
                     /gene="carA"
                     /locus_tag="PKN91_09100"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012053752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNS28270.1"
                     /translation="MSVPAVLALADGTIFRGQSIGAKGNTTGEVVFNTAMTGYQEILT
                     DPSYSRQIVTFTYPHIGNTGTTPEDLEAQIVHASGLVIRDLPRLASNWRAVGSLGEFL
                     ERAKTVAIAGIDTRRLTRILRERGAQAGCIMTGEKIDPAAAVRAAGKFPGLKGMDLAK
                     VVSVRRPYQWNEGSQWGNGESQRALRAHQRLHVVAYDFGVKRNILRMLADHGCRMTVV
                     PAQTSAAEVLALQPDGIFLSNGPGDPEPCGYAIDAIREFLQTDLPMFGICLGHQLLGL
                     ACGAGTVKMKFGHRGANHPVQDLATGRVMITSQNHGFAVDEATLPPHVKATHRSLFDG
                     SLQGLAWIDRPVFGFQGHPEASPGPHDMRPLFELFSQLMVRRLQNQLRQRAS"
     gene            complement(2481..3230)
                     /gene="dapB"
                     /locus_tag="PKN91_09105"
     CDS             complement(2481..3230)
                     /gene="dapB"
                     /locus_tag="PKN91_09105"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNS28271.1"
                     /translation="MTRVAILGGVGRMGRALVACAEGLDIAVTAVVASARSAALGQDA
                     GEAAGVRRLGVAITSDLAAALRGAQVVVDFSSPHAARDHVAQCAEARVPLLLGTTGLP
                     DGIEADFARAAERIPLLVAANTSLGITLLLDLVQRAARALPGGFDVSIIEAHHRAKRD
                     APSGTALALGRVIETAREGEVGYAVVRGGDIVGEHDVQFIGAGERLTLGHQATDRAIF
                     ARGALQAAAWLVGQAPGRYSMADVLGIKSAC"
     gene            complement(3227..4366)
                     /gene="dnaJ"
                     /locus_tag="PKN91_09110"
     CDS             complement(3227..4366)
                     /gene="dnaJ"
                     /locus_tag="PKN91_09110"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNS28272.1"
                     /translation="MAKRDYYQVLGVAKNASEADIKKAYRRLAMKLHPDRNPGDKEAE
                     EKFKEAKEAYEVLSDDQKRATYDQLGYAGIEAARQGGGAGGFNPGDAFGDIFGDVFGD
                     IFGGGRRGGGRSQVFRGADLRYELELDLHDAVFGKSVEIEIPKLTECESCHGSGAAKG
                     STPVNCETCNGSGQVRISQGFFQLQQTCPKCRGSGKIIRNPCDTCLGQGRVRRVKTLA
                     VKVPAGVDTGDRIRLGGEGEAGRNGGPPGDLYVEVSVRDHPIFERDGAHLSCEVPVSF
                     AIAALGGAVDVPTLDGHVSLKVPAETQSGRVFRLRDKGVKPVRGGARGDLFCRVVVET
                     PVNLTTEQRELLSKLDESLRKGGSRHAPREKSFFDGVKRFFMGEA"
     gene            complement(4448..6385)
                     /gene="dnaK"
                     /locus_tag="PKN91_09115"
     CDS             complement(4448..6385)
                     /gene="dnaK"
                     /locus_tag="PKN91_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNS28273.1"
                     /translation="MGKVIGIDLGTTNSCVAVMDGGKSRVIENSEGDRTTPSIVAFTK
                     DDEVLVGQSAKRQAVTNPHNTLFAVKRLIGRKFDDAVVQKDVDMVPYRIVKADNGDAW
                     VDINGKKMAPPEVSARVLMKMKKTAEDYLGEEVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLNVKRIINEPTAAALAYGLDKDGGDRKIAVYDLGGGTFDVSIIEIAAVDGERQFEV
                     LSTNGDTFLGGEDFDLKIIHFLADEFLKESGVDVRKDPLAMQRLKEAAEKAKIELSSS
                     QQTDVNLPYITADASGPKHLNIKLTRAKLESLVEDLVRRTIDPCKVALKDAGLSASEV
                     SEVILVGGQTRMPLVQKYVKDFFGKDPRKDVNPDEAVAVGAAIQAGVLAGEVKDVLLL
                     DVTPLSLGIETLGGVMTKLIEKNTTIPTKTSQVFSTADDNQTAVTIHVLQGERDRAGD
                     NKSLGKFDLSDIPPAPRGMPQVEVTFDIDANGILHVSAKDKATGKEQKIVIKASSGLT
                     EDEIKRMVRDAEAHAEEDRKFRELVEARNRAEQMIHAVEKSLTDLGEKVDAGERAKVE
                     SALSDLRSAVKGDDQAAIAKKTEALTQASAAIAQQAYAAAQGGAATGDAAGAGEAPKG
                     AEGDVVDAEFEEVDDKGRRAS"
     gene            complement(6446..6973)
                     /locus_tag="PKN91_09120"
     CDS             complement(6446..6973)
                     /locus_tag="PKN91_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNS28274.1"
                     /translation="MVDADSGTPAAAAPEPVAGREQDLLARLAEAEAAATRAQEQYLR
                     AVAEMDNVRKRAQRDIEAANRYGLERFAQELLPVKEGLDLAVENADKADRESLVAGQV
                     ANRQLLARAFERLGIVELDPVGEPFDPEQHEAMLVQPSATAEPDSVLAVVQRGYSLNG
                     RLLRPARVIVAKPLE"
     gene            complement(7024..8079)
                     /gene="hrcA"
                     /locus_tag="PKN91_09125"
     CDS             complement(7024..8079)
                     /gene="hrcA"
                     /locus_tag="PKN91_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNS28275.1"
                     /translation="MSSDHRDEALGERAQHLLRVLVESYIRDGQPVGSRTLSRESGLA
                     LSSATIRNVMSDLEALGFVSSPHTSAGRVPTDKGYRFFVDTLLKVQPLDSADVAEIKR
                     QLSAGVDDPKALVNVASRLLSSVTHLAGVVTLPRTAVQAVSQVEFVGLSENRILVILV
                     FNDREVQNRIIKADRHYSPDELKRAANYLTEQFRGRSLNQGRQEILRQLKETRERLHE
                     VMGSAIAVAQQVIAPEESQLEYVIAGETNLMDFAELSSVERLRRLFEAFNEKRDFLHL
                     LDQSLRAEGVQIFIGHESGYQVLDDYSIVTAPYSADNEVVGVLGVIGPTRMAYERVIP
                     IVDLTARVLGAALNSRR"
     gene            8193..9095
                     /locus_tag="PKN91_09130"
     CDS             8193..9095
                     /locus_tag="PKN91_09130"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNS28276.1"
                     /translation="MPRARKTFALVGRFSDPRVAESAAIAVAHLAERGVATLVADDGE
                     SARFEAGTAQVVRVAESELAGRADAVLAIGGDGTLLYAARLVAGRDVPLLGINRGRLG
                     FLTDVLPQDLTASIDAVLDGSCDIDVRPLLEARLSRAQGAPLVQQALNDVVVQKHETG
                     RMIDFESWIDGRFVNTHGADGVVIASATGSTAYALSCGGPIVEPHLDVVVVAPICPHT
                     LSDRPIVIPRRSTVEIALVDRPNTRAHVTVDGIVLDDLLAGDRLAVGPSPHSIRLLHP
                     KGYDYYRLLRSKLHWGRGGNEHNR"
     gene            9101..>9671
                     /locus_tag="PKN91_09135"
     CDS             9101..>9671
                     /locus_tag="PKN91_09135"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS28277.1"
                     /translation="MLTHLQIRDFAIVDAAEIEFRPGLTVLTGETGAGKSIIVDALQL
                     LAGGRAGSEVVRHGAERAEVSASFDVGDPSAPLARLLREQSIDAGDELTIRRVVSSDG
                     RSRAYLNGQSVPAQLLREVGVLLVDIHGQHEFQSLVRPASQRELLDASGGLEGLAAEV
                     AGLQRVWLTLLNRTLDLESRVRDRDARTEL"
BASE COUNT         1458 a         3323 c         3189 g         1701 t
ORIGIN      
        1 cttggtgacg acgtagtcga tcgccggctc gaaggacgcc ggcgtggcac cgccggtgat
       61 gtcgttctgc agctcatcga gcgtgtagcc gatggcgagc ttggcggcga tcttggcgat
      121 cgggaagccg gtcgccttcg aggcgagcgc cgaggagcgc gacacgcgcg ggttcatctc
      181 gatgatcagc atgcgcccgt cggccggatt gaccgagaac tgcacgtttg agccgccggt
      241 gtcgaccccg atcttgcgca gcaccgcgat cgaggcatcg cgcatgcgct ggtactcctt
      301 gtcggtcagc gtctgcgccg gcgccaccgt gatcgaatcc ccggtgtgca cgcccatcgg
      361 gtcgagattc tcgatcgagc agatgatgat gcagttgtcc ttgcggtcac gcaccacctc
      421 catctcgtat tccttccagc cgatgactga ttcttcgatc agcacctcgc tggtcggcga
      481 agcctcgagg ccgcgtgtga cgatcgcctc gagctcctcg cggttgtagg cgatgccgcc
      541 gcccgagccg ccgagcgtga acgaggggcg gatcacgcac ggatagccga tctcggccac
      601 cacggcgagc gcatcctcga ggctcttggc gatgtgcgag cgcggcgact cgaggccgat
      661 ctcgcgcatc gcattcctga agcgttcgcg gtcttcggcc atgtcgatcg cggcccgcga
      721 ggcggcaatc agctcgacgc cgtattcctc gagtacgccc tggcggtcga ggtcgagcgc
      781 ggtattgagc gccgtctgcc cgcccatcgt cggcagcagc gcgtccgggc gctccttggc
      841 gatgatgcgc gcgatcgtgc gccagttgat cggctcgatg taggtggcat cggccatctc
      901 cgggtcggtc atgatcgtcg ccggattcga attgacgagg atcacccggt aaccctcttc
      961 gcgcagtgcc ttgcaggcct gggcgccgga atagtcgaac tcgcaggcct ggccgatgac
     1021 gatcggtccg gcgccgatga tcaggatgct ctggatgtcg gtacgtttgg gcatggggtc
     1081 aggaggcgcg ctggcgcaac tggttctgca ggcggcgcac catcaactgg gaaaacaact
     1141 cgaacagcgg acgcatgtcg tgcggaccgg ggctcgcctc gggatgcccc tggaagccga
     1201 acaccggccg gtcgatccac gcgaggccct gcagcgatcc gtcgaacagc gagcgatggg
     1261 tcgccttgac gtgcggcgga agcgtcgcct cgtcgaccgc gaaaccgtgg ttctggctgg
     1321 tgatcatcac gcgcccggtc gcgaggtcct gtaccgggtg attggcgccg cgatggccga
     1381 atttcatctt cacggtgcct gcaccgcagg cgagtccgag caactggtgc ccgaggcaga
     1441 tgccgaacat cggcaggtcg gtctgcagga attcgcggat cgcgtcgatc gcgtaaccgc
     1501 agggttccgg gtctcccgga ccgttcgaca ggaagatgcc atcgggctgc agcgcgagca
     1561 cttccgcggc gctggtctgt gccggcacca ccgtcatgcg gcagccatgg tcggcgagca
     1621 tgcgcaggat gttgcgcttc actccgaagt cgtaggcgac gacgtgcagc cgctggtgcg
     1681 cgcgcagcgc gcgttgtgac tcgccgttgc cccactgtga accttcattc cactggtagg
     1741 gccgccgcac gctgacgacc ttggcgagat ccatgccctt cagcccgggg aacttgcctg
     1801 ccgcacgcac cgcggcggcc gggtcgatct tctcgcccgt catgatgcag ccggcctgcg
     1861 cgccacgctc gcgcaggatg cgggtcaggc ggcgcgtgtc gatgccggcg atcgccaccg
     1921 tcttcgcgcg ctcgaggaat tcaccgagcg agccgaccgc gcgccagttc gatgcaagcc
     1981 gcggcaggtc gcggatcacg aggcccgaag cgtgcacgat ctgcgcctcg aggtcctcgg
     2041 gcgtcgtgcc ggtgttgccg atatggggat aggtgaaagt gacgatctga cgggaatacg
     2101 acgggtcggt gaggatttcc tggtaccccg tcatggcggt attgaaaacg acttcgccgg
     2161 tggtgttgcc tttggctccg atggactggc cgcggaagat cgttccatcc gccagcgcca
     2221 gtactgcggg tacgctcacc aaatctttcc cctgtctttc gttccgcgcc cctcgagacg
     2281 caagcgggtt gcgccgagtg aaatcgccgg aggcatatgt acaaagcggg aggagttctt
     2341 tcgaacatcc tcccgccttg cacggactaa cccgacaatc ggattacgcc caaagtttaa
     2401 ggtccaccgc ccggggtgtc catgtgaata cggccgggca agttctccac cgaagctcac
     2461 aaatgccttc ggattaccgt ctaacaggct gattttattc ccagtacgtc ggccatgctg
     2521 tagcgacccg gcgcctgccc gaccaaccag gcggccgcct ggagggcccc ccgggcaaaa
     2581 atcgcccggt cggtcgcctg gtgaccgagg gtcagccgct cgccggcccc gatgaactgc
     2641 acgtcgtgct ccccgacgat gtcaccgcca cgcacgaccg catagccgac ctcaccctcg
     2701 cgcgccgttt cgatgacgcg tccgagcgcg agcgcggtcc ccgacggggc atcgcgcttg
     2761 gcgcggtgat gcgcctcgat gatcgacacg tcgaacccgc cgggcaaggc gcgcgccgcg
     2821 cgctgcacca ggtcgagcag cagcgtgatg ccaagactcg tgttcgcagc gacgagcagc
     2881 ggaatgcgtt cggccgcgcg ggcgaaatcc gcctcgatgc catcaggcaa cccggtggta
     2941 cccagcagca gcggcacgcg cgcctcggcg cactgcgcga catgatcgcg cgccgcatgc
     3001 gggctcgaga aatcgacgac gacctgcgcg ccgcgcagcg cggcggcgag gtccgaggta
     3061 atcgcgacgc ccagccggcg cacgccggcc gcctcacccg cgtcctggcc gagcgcggcg
     3121 ctgcgcgcgg aggcgacgac ggcggtgacc gcgatgtcga gcccctcggc acaggcgacg
     3181 agcgcgcggc ccatgcgtcc gacgcccccg aggatcgcga cgcgggtcac gcctccccca
     3241 taaagaaacg cttcacgccg tcgaagaacg acttctcgcg cggcgcatgg cggctgccgc
     3301 ccttgcgcag ggactcatcg agcttcgaca agagttcgcg ttgctccgtc gtgaggttca
     3361 cgggtgtttc gaccacgacg cggcagaaca ggtcgccccg ggccccgcca cgcaccggct
     3421 ttacgccctt gtcgcgcagg cgaaagacgc gtccggactg ggtctcggcc ggcaccttca
     3481 gcgaaacatg cccgtcgagc gtcggcacgt cgaccgcccc gccgagcgcg gcgatcgcga
     3541 agctgaccgg cacctcgcag ctgaggtgcg cgccgtcgcg ttcgaagatc gggtggtcgc
     3601 ggacgctcac ttcgacatag aggtcgcccg gcggtccacc gttgcgcccc gcttcgcctt
     3661 cgccgccgag gcggatgcga tcgcccgtgt cgactcccgc cggcaccttg accgccagtg
     3721 tcttcacgcg gcgcacgcgc ccctgcccga ggcaggtatc gcacggattc ctgatgatct
     3781 tgccgctgcc gcggcacttg ggacaggtct gctgcagctg gaagaatccc tgcgagatcc
     3841 gcacctggcc gctgccgttg caggtttcgc agttgaccgg cgtggagccc ttggccgcac
     3901 cgctcccgtg gcagctctcg cactcggtga gcttcgggat ctcgatctcg acgctcttgc
     3961 cgaacactgc atcgtgcagg tcgagctcga gctcgtagcg caggtcggcg ccgcgaaata
     4021 cctgcgagcg gccgccgcca cggcgaccgc cgccgaagat gtcaccgaac acgtcgccga
     4081 agatgtcacc gaaggcatcg ccggggttga agcctcccgc gccaccgccc tgacgcgcgg
     4141 cctcgatgcc ggcgtagccc agctggtcgt aggtcgcccg cttctggtcg tccgagagga
     4201 cctcgtaggc ttccttcgct tccttgaatt tctcctcggc ttccttgtca cccggattgc
     4261 ggtccgggtg gagcttcatt gcgagccggc ggtaggcctt tttgatgtcg gcctcgctgg
     4321 cgttcttcgc cacgccgagg acctggtagt aatcgcgctt cgccatgcgt ttccagtcga
     4381 tacgtgaaag ccgccgcgca cccttgcggt gcgcggcagc ctgtctgcct ccgcccggga
     4441 ccgcgcgtta cgacgccctg cggcccttgt cgtccacctc ttcgaactcc gcgtcgacca
     4501 cgtcgccctc ggcgcccttc ggcgcctcgc cggcgccggc cgcgtcaccc gtggccgcgc
     4561 caccttgcgc cgccgcgtat gcctgctgcg cgatcgcggc ggaggcctgc gtcagcgcct
     4621 cggtcttctt ggcgatcgcg gcctggtcgt cgcccttgac tgcgctgcgc aggtccgaca
     4681 gcgccgactc gaccttggcg cgctcgcccg cgtcgacctt ctcgccgagg tccgtgagcg
     4741 acttctcgac ggcgtggatc atctgctcgg cgcggttgcg tgcctcgacg agttcgcgga
     4801 acttgcggtc ctcctcggcg tgcgcctcgg cgtcgcgcac catgcgcttg atctcgtcct
     4861 cggtgaggcc gctcgaggcc ttgattacga tcttctgttc cttgccggtg gccttgtcct
     4921 tcgccgacac gtgcaggatg ccgttcgcgt cgatgtcgaa agtgacctcg acctgcggca
     4981 tgccgcgcgg cgccggcgga atgtcggaca ggtcgaactt gccgagcgac ttgttgtcgc
     5041 cggcgcggtc gcgctcgccc tgcagcacgt ggatagtcac cgcggtctgg ttgtcgtccg
     5101 cggtcgagaa cacctgcgag gtcttggtcg ggatcgtggt gttcttctcg atgagcttgg
     5161 tcatcacgcc gcccagcgtc tcgatgccga gcgacagcgg ggtgacgtcg agcagcagca
     5221 cgtccttcac ttcgccggcg agcacgcccg cctggatcgc ggcgccaacg gcgaccgcct
     5281 cgtccgggtt gacgtccttg cgcggatcct tgccgaagaa gtccttgacg tacttctgca
     5341 cgagcggcat gcgggtctga ccgccgacca ggatcacctc ggacacctca ctcgccgaca
     5401 ggcccgcatc cttcagtgcg accttgcacg gatcgatggt gcgcctgacg agatcctcga
     5461 cgagcgactc gagcttggcg cgggtgagct tgatgttcag gtgcttcggc cccgacgcat
     5521 cggccgtgat gtacggcagg ttgacgtcgg tctgctggct cgaggacagc tcgatcttgg
     5581 ccttctcggc cgcttccttc aggcgctgca tcgcgagcgg gtccttgcgc acgtcgacgc
     5641 cggactcctt caggaactcg tccgcgagga aatggatgat cttgaggtcg aagtcctcgc
     5701 cgccgaggaa ggtgtcgccg ttggtcgaga gcacttcgaa ctggcgctcg ccgtctaccg
     5761 cggcgatctc gatgatcgac acgtcgaagg tgccgccacc caggtcgtac acggcgatct
     5821 tgcggtcgcc gccgtccttg tcgaggccgt aggcgagcgc cgccgcggtc ggctcgttga
     5881 tgatgcgctt gacgttgagg ccggcgatgc ggccggcgtc cttggtcgcc tggcgctgcg
     5941 agtcgttgaa atacgcgggt acggtgatga ccgcctcggt gacttcttcg ccgaggtagt
     6001 cctcggccgt cttcttcatc ttcatcagca cgcgcgcgga aacttcgggc ggcgccatct
     6061 tcttgccgtt gatgtcgacc caggcgtcgc cgttgtcggc cttgacgata cggtagggga
     6121 ccatgtcgac gtccttctgg acgacggcat cgtcgaactt gcggccgatc agccgcttga
     6181 cggcaaacag agtgttgtgc ggattggtca ccgcctggcg cttggccgac tgaccgacga
     6241 gcacctcgtc gtccttggtg aacgccacga tcgacggggt cgtgcgatcg ccctcgctgt
     6301 tctcgatgac gcgcgacttg ccgccgtcca tgaccgccac gcacgagttg gtcgtgccga
     6361 ggtcgatgcc gattaccttg cccatgaatg tctccgcaac tcgttaactg aaaccctgta
     6421 gcccgagatg gggctgctcc ctgtcctatt caagcggttt ggcgacgatg acccgcgccg
     6481 ggcgcagcag gcgtccgttg agcgagtatc cgcgctgcac gacggcgagc accgaatccg
     6541 gctcggcggt ggccgagggc tgcaccagca tcgcctcgtg ctgttcgggg tcgaagggct
     6601 cgccgaccgg gtccagctcg acgatgccga ggcgctcgaa cgccctcgcc agcagctggc
     6661 ggttggccac ctggccggcc acgagactct cgcgatcagc cttgtcggca ttctcgaccg
     6721 ccaggtcgag gccctccttc acgggcagca gttcctgcgc aaaacgctcg aggccatagc
     6781 ggttggccgc ctcgatgtcg cgctgggcac gcttgcgcac gttgtccatc tccgcgacgg
     6841 cgcgcagata ctgttcctgg gcgcgggtgg ccgccgcctc ggcctccgca aggcgcgcga
     6901 gaagatcctg ttcgcgccca gccaccggct cgggcgccgc cgccgccggc gtgccactgt
     6961 ccgcatcgac catcaataag tccctcgaat caaatagtta gggctttgcc ccggatatgg
     7021 ggatcagcgg cgcgaattca aggccgcgcc gagcaccctg gccgtgaggt ccacgatcgg
     7081 aatcacccgt tcataggcca tgcgcgtggg accgatgacc ccaagcacgc cgaccacttc
     7141 gttgtccgcc gagtaaggcg cggtgacgat gctgtaatcg tcgagtacct ggtagcccga
     7201 ctcgtgcccg atgaagatct gcaccccctc ggcgcgcagg ctctggtcga gcagatgcag
     7261 gaagtcgcgc ttctcgttga acgcctcgaa caggcgtcgc aggcgctcga cgctcgagag
     7321 ctcggcgaag tccatcaggt tggtctcgcc ggcgatcacg tactcgagct gcgattcctc
     7381 cggggcgatg acctgctggg cgaccgcgat cgcgctgccc atgacctcgt gcagccgttc
     7441 gcgcgtttcc ttcagctggc gcaggatctc ctgccgtccc tggttcaggc tgcggccacg
     7501 gaactgctcg gtgaggtagt tggcagcgcg tttgagttca tcgggcgagt agtggcgatc
     7561 ggccttgatg atgcggttct gcacctcgcg atcgttgaac accaggatca cgaggatgcg
     7621 attctcggac aggccgacga attccacctg cgagacggcc tgcacggcgg tgcgcggcag
     7681 ggtgacgacc cccgcgaggt gcgtgacgct cgagagcagg cgcgaggcga cgttgaccag
     7741 cgccttcgga tcgtcgacgc cagccgacag ttggcgcttg atctcggcca cgtcggccga
     7801 atcgagcggc tgcaccttga gcagtgtatc gacgaagaag cgatagccct tgtcggtcgg
     7861 gacgcgcccc gccgaggtgt gcggcgagga gacgaacccg agcgcctcga ggtcggacat
     7921 cacgttgcgg atcgtcgccg aactcagcgc caggcccgat tcgcgcgaca gggtgcgcga
     7981 accgaccggc tggccgtccc ggatatagct ctcgaccaga acccgcagca ggtgctgcgc
     8041 ccgctcgccc agggcctcgt ctcggtgatc gctcgacata tcgatccacg ctagcggaga
     8101 tcgcgaaacc gtgcaatcgg tcgccatacg actcgttggc cggcccgggc ccgcatgtta
     8161 aaaaggtccg cattgccacg acccggtgaa gcatgccccg agcccggaag acctttgccc
     8221 tcgtcgggcg cttctccgac ccccgcgtcg cggagagcgc cgcgatcgca gtcgcccacc
     8281 tggcggagcg cggcgtggcc acactggttg ccgacgacgg cgagtctgcc cggttcgagg
     8341 ccggcacggc gcaggtggtg cgggtcgccg agtccgaact cgccgggcgc gccgacgccg
     8401 tgctcgcgat cggcggcgac ggcacactgc tctacgcggc gcgactcgtg gcgggtcgcg
     8461 acgtgccgct gctcggcatc aatcgcggcc ggctcggctt cctcaccgac gtgctgccgc
     8521 aggacctcac cgcatcgatc gatgccgtgc tcgatggctc ctgcgacatc gatgtgcggc
     8581 cgctgctcga ggcgcgcctg tcgcgcgcgc agggcgcgcc gctggtgcag caggccctga
     8641 acgatgtggt cgtgcagaag cacgaaaccg gccgcatgat cgatttcgag tcatggatcg
     8701 acggccgctt cgtcaacacg cacggcgccg acggcgtcgt gatcgcgagc gccaccggtt
     8761 cgaccgccta cgcgctctcc tgcggcgggc ccatcgtcga gccgcacctc gatgtcgtgg
     8821 tcgtcgcccc gatctgcccg cacacgctgt ccgaccgccc gatcgtcata ccgcgccgct
     8881 cgaccgtgga gatcgcgctg gtcgaccggc cgaacacgcg cgcgcacgtc accgtcgatg
     8941 gcatagtcct cgatgacctg ctcgccggcg accgcctcgc cgtgggaccc tcaccccact
     9001 cgatccggct gctgcacccg aagggctacg attactaccg ccttctgcgc tcgaagctgc
     9061 actggggccg cggcggcaac gagcacaacc ggtaaaccac atgctcactc acctgcagat
     9121 ccgggatttc gcgatcgtcg atgccgcgga aatcgagttt cgcccggggc tcaccgtgct
     9181 caccggcgag accggcgccg gcaagtcgat catcgtcgat gcgctgcagc tgctggccgg
     9241 cggccgcgcc ggtagcgagg tcgtgcgcca tggcgccgag cgcgccgaag tcagcgcgag
     9301 tttcgatgtc ggcgatccgt cggcgccgct cgcccgcctg ctgcgcgagc aatcgatcga
     9361 tgcgggtgac gaactgacga tccgccgggt cgtctccagc gacggacgaa gccgcgccta
     9421 cctgaacggc cagtccgtac cggcgcaact gctgcgcgag gtcggcgtgt tgctcgtcga
     9481 catccatggc cagcacgagt tccagtcgct cgtgcgaccg gcctcgcagc gcgaactgct
     9541 ggatgcctcg ggcggcctcg aagggctcgc cgccgaagtc gccggactgc agcgcgtatg
     9601 gctcacgctg ctcaaccgta cgctcgatct cgagagtcgc gtgcgcgatc gcgatgcacg
     9661 caccgagctc g
//
LOCUS       DAOQVY010000093         9313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_8251_length_9313_cov_5.960467, whole genome shotgun sequence.
ACCESSION   DAOQVY010000093 DAOQVY010000000
VERSION     DAOQVY010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9313
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8251_length_9313_cov_5.960467"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /gene="speA"
                     /locus_tag="PKN91_09140"
     CDS             complement(<1..1464)
                     /gene="speA"
                     /locus_tag="PKN91_09140"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HNS28278.1"
                     /translation="MVAEAKSGLKQGWSTDDSAELYQVRAWGKGYFAVNAAGHVVVRP
                     DTTPRNEIDLFEVIEGLKARDLTTPVVVRFSDILAHRLGHIASAFAHAIAENEYQNSY
                     AAVFPIKVNQQRLVVEEVFRYGAEHGFGLEVGSKPELLAVMAMTEGAADRLIVCNGFK
                     DDSYIEAVVLATKLGRTIIPVVENFSELSLILKHAEAYAVRPRIGVRVKLSSEGSGRW
                     RDSAGEKSKFGLFITEILEVIEVLRARDMLDCLQLVHCHPGSQLQDIRRVKDAVNELA
                     HVYAELSNLGAGLRYIDVGGGLGVDYDGSGTNFPSSMNYTINEYANDVVYRIASVCNA
                     RGIAHPMIISESGRAIAAQHSVLIFNTLGTSALDRFRVSGKVEADYDGSAELPLPVQD
                     LFEAYASISARTLVECYHDAITAREQVLQMFNLGLVSLAVRGLAERLFWATCARIRDM
                     CRKLDETPEELAGLEEILSDIYFCNFSVFQSLPDSWAI"
     gene            complement(1502..2350)
                     /locus_tag="PKN91_09145"
     CDS             complement(1502..2350)
                     /locus_tag="PKN91_09145"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNS28279.1"
                     /translation="MYSTLIDVAQLGALLGRPEVVILDCRFELADPDAGAAAYAQGHI
                     PGAQYAHLERDLSGPAGPTTGRHPLPDPERLAITFGRWGINANVQVIAYDAGPGAYAA
                     RAWWMLRWLGHEAIAVLNGGFAAWQAAGMAVETIARRRDPRLYVPQLQADRAIGAADL
                     AAELESGRVLLVDARGADRFAGRNETMDAVAGRVPGARNRPFTENLAPDGRFLPAETL
                     REQWTALLAGRPVGELVSMCGSGVTACHNLLALEHADLPGGRLFPGSWSEWITDPSRP
                     VATGDG"
     gene            2396..3073
                     /locus_tag="PKN91_09150"
     CDS             2396..3073
                     /locus_tag="PKN91_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNS28280.1"
                     /translation="MIDLYWGSGSPYCWRVLLALEHKRLEYVSHQLQFSRQEHKAPHI
                     VAMNPRGRLPILKHDDYVVFESVAVLHYLDLKYPEPPIFGRTPEEAGVIVRVICEFEA
                     YAQEHLAKITRALFGAGVGEDNVGELTRAMLVVASEARTIEGRLSKGDWIVGDAYSAA
                     DMVIYPAIMVLLRALQRPGAEVLASRFMPVAVNYPSLARWLDRVAALPGYEKTYPPHW
                     LASARHG"
     gene            3113..>4414
                     /locus_tag="PKN91_09155"
     CDS             3113..>4414
                     /locus_tag="PKN91_09155"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNS28281.1"
                     /translation="MADKVYIEFPGRIVLIGFGSIGQGVMPLILRHIGIDPSRISVVT
                     ADDRGRKEAEEYGIKFVKAPLTRENYRRILDANIGRGDFVLNVSVDVSSVALVKYCWE
                     RGAMYLDTCIEPWAGGYTSPTMTPAQRTNYALREAALALRPGSDRAPTAVITHGANPG
                     LVSHFVKQALINIAGDLGVEAAPRNRAEWAKLARQLGVTTIHIAERDTQVSSQPKKVN
                     EFVNTWSIDGFVGEGCQPAELGWGTHERNFPRDGKRHEGGCGAAIYLMQPGAATRVRT
                     WTPNAGHIMSFLITHGESISIADYFTVIESMQPVYRPTVHYAYHPSDAAVLSVWELQG
                     RNWQLQDNTRIMMDDIVSGMDELGVLIAGHKKNAYWYGSQLTVEEARKLAPHNQATSL
                     QVTSSCLAGMVWAMENPNCGLMEPDDLDYKRLLEICRPYLGT"
     assembly_gap    4415..4514
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4515..4952)
                     /gene="greB"
                     /locus_tag="PKN91_09160"
     CDS             complement(<4515..4952)
                     /gene="greB"
                     /locus_tag="PKN91_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017055625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HNS28282.1"
                     /translation="MSRYRPPQTPGSRFITPAGHARLKAELDQLWRVERPQVTQAVAE
                     AAAQGDRSENAEYTYGKRRLREIDRRVRFLRQRLEGMVVVDRPPDDRERVYFGAWVTL
                     EGEDRRELRVRIVGPDEFDAAPGFVSMDAPLARALLGKRLDDEV"
     gene            5067..5264
                     /locus_tag="PKN91_09165"
     CDS             5067..5264
                     /locus_tag="PKN91_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28283.1"
                     /translation="MSARYYTHFVTPGDEARAAHEWSGVVELGRELDQRAGAGELARL
                     LAQSFELEFEDIRILHWARLH"
     gene            5366..8305
                     /locus_tag="PKN91_09170"
     CDS             5366..8305
                     /locus_tag="PKN91_09170"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNS28284.1"
                     /translation="MRFLKPLAWTALVIALLIGAYAWAGYWLAPRLVQSKLPPAISAA
                     TGLRLTLGEVAIRPFSLSADLRDVSLTEPDGTALLAAQRLYIDARLAALWRRGVILES
                     LLLEQPALNLILREDGSVNLVTAFESKAPPPPPGEEGRPLPVSIDDIRIQGGVIDAAD
                     LRRATPLQRRLEPINVRVQNFTTSAGDEGDFSITGRDEDGGEITLRGQLAVRPFALHG
                     DLEVKGLSARTAWEVAGRYGRVAPPQGVIDFRTLYQIAAADDGMRIALDALDFEAREL
                     AVRPAGGEEDWVKVAALSATGASVDVRESLVKLPDLRVQGLDVSAWTEADGGVNLAAL
                     APAEAAPAEPAAAVVPAESAAPVASPEPAEPAKGWRIEAPMLRVTAARIAFEDRNAPQ
                     PVRYVLAPLELDIDGYASGAPAVTAALRSGFNETGKLAIEGQWRLDASGGAFTVAAET
                     LPVVFLQPYLERTTDLVLRSGDVSVEGKVAIDRAAEGKPRIGFEGNATLARFHSVDRE
                     LREDFVRFRRLAASRISYSSAPTTLRVRDLLARGAYFKLVISPESTTNVAEVLSPPRL
                     RKDTAAETAATADAGAAKTARAPARKLRIDKVRVVDSAAFFADLSIRPSFSTGIEEFN
                     GTITGLSSDPAARADVVLDGKVDRYAPVEVRGEVNLLSSTTFTDLTADFHNIELPTFT
                     PYSGKFMGYQIEKGKLDAHFRYLIDHRKLDAQHKFKLDQFTLGDRVDSDDAMKIPMKL
                     AVALLKDRHGVIDIDLPVSGSLDDPTFRVGPLVWKALRNLLVKIITAPFALIGSLFGA
                     GEEVRFVDFAYGSATLDAAAGEQLANVGKALTDRPQLKLGVPLAFDAARDRAALVEQR
                     MIELLGGPEAHALRETDTGAYQEALDRAWRELSGERRAPRPEREKDEAKEAHALRAIE
                     AGETALRERIVIADAALADLAKQRADAVREALVTGGGLDPERIFLVAGEPAADATSGV
                     RLALQVE"
     gene            8324..>9313
                     /locus_tag="PKN91_09175"
     CDS             8324..>9313
                     /locus_tag="PKN91_09175"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HNS28285.1"
                     /translation="MRIPFLFAAAAILAACAAPTPAPVPETTEIAVPAPPLASGLVLD
                     GYDRSVRPQDDLHEFAGGKWLAETAIPADRSSWGTFAILDERAERQVREIAEQAAATP
                     VHVPGSDLQKIGDLYASFMDEQAIEAAGLAPLEADFARIRALATHADVVRYIGESQRR
                     LQRVPLGYYVGQDAGDATRYIGTVQQAGLSMPDRDYYLVQDKRQAENRKRHLAYVEQM
                     LTMAGAKDTAARAQRIQQLETRIAAIQWSKVQNRDPRATYNRTSPIELAKLTPAFDWT
                     AFFTAAGAPIEAVDINQPTYVEALAKLIAATPLPQWREYFFFQLLDVSAPYLAA"
BASE COUNT         1483 a         3122 c         3168 g         1440 t
ORIGIN      
        1 gatcgcccag ctgtcgggca gcgactggaa caccgagaag ttgcagaagt agatgtcgga
       61 gaggatctcc tcgagcccgg cgagctcctc gggcgtctcg tcgagcttgc ggcacatgtc
      121 gcggatgcgt gcgcaggtcg cccagaacag ccgctcggcg aggccgcgca ccgcgagcga
      181 cacgaggccc aggttgaaca tctgcagcac ctgttcgcgc gcggtgatcg cgtcgtggta
      241 gcactcgacg agcgtgcgcg cgctgatcga cgcgtacgcc tcgaacaggt cctgcaccgg
      301 cagcggcagc tcggcgctgc cgtcgtagtc cgcctccacc ttgcccgaga cgcggaaccg
      361 gtcgagcgcc gaggtgccga gcgtgttgaa gatgagcacg ctgtgctgcg cggcgatcgc
      421 gcggccggat tcgctgatga tcatcgggtg cgcgatgccg cgcgcgttgc acacgctcgc
      481 gatgcggtac acgacatcgt tcgcgtactc gttgatcgtg tagttcatcg aggaggggaa
      541 attggtgccg ctgccgtcgt agtcgacacc cagcccgccg cccacgtcga tgtacctgag
      601 cccggcgccg aggttcgaca gctcggcata cacgtgcgcg agttcgttca cggcgtcctt
      661 gacccggcgg atgtcctgca gctggctgcc cgggtggcag tgcacgagct gcaggcagtc
      721 gagcatgtcg cgcgcacgga gcacctcgat cacttcgagg atctcggtga tgaacaggcc
      781 gaacttcgac ttctcgcccg ccgaatcgcg ccagcgcccc gagccctcgc tcgagagctt
      841 gacgcgcacg ccgatgcgcg ggcgtacggc ataagcctcg gcgtgtttca ggatcaggct
      901 gagctcggag aagttctcga ccaccgggat gatcgtgcgg ccgagcttgg tcgcgaggac
      961 cactgcctcg atgtagctgt cgtccttgaa gccgttgcag acgatcaggc ggtcggcggc
     1021 accctcggtc atcgccatca ccgcgagcaa ctcgggcttc gagcccacct cgaggccgaa
     1081 gccgtgctcg gcgccatagc ggaagacttc ctcgaccacc aggcgctgct ggttcacctt
     1141 gatcgggaac acggcggcgt aggagttctg gtactcgttc tcggcgatcg cgtgcgcgaa
     1201 ggcgctggcg atatggccca gccggtgcgc gaggatgtcg gagaagcgca cgacgaccgg
     1261 cgtcgtgagg tcgcgggcct tcaggccttc gatgacctcg aacaggtcga tctcgttccg
     1321 tggcgtggtg tccggtcgca cgacgacgtg gcccgccgca ttgacggcga agtagccctt
     1381 gccccaggcg cggacctggt acagctcggc cgagtcgtcg gtgctccagc cctgcttcag
     1441 gcctgacttc gcttcagcaa ccacgggtca ctcccggaaa aagggcgcgc acgatagcaa
     1501 atcagccatc acccgtcgcg acgggtcgcg acgggtcggt aatccactcg ctccaggatc
     1561 cggggaagag tcgcccgcct ggcaggtcgg cgtgttcgag cgcgagcagg ttgtggcagg
     1621 cggtgactcc cgagccgcac atcgagacca gttcgccgac cggacgaccc gcgagcagtg
     1681 cggtccactg ttcgcgcagc gtctcggcag gcaggaagcg gccgtccggc gcgaggttct
     1741 cggtgaacgg acgattgcgc gcgcccggca cgcggccggc gaccgcgtcc atcgtctcgt
     1801 tccggccggc gaagcggtcg gcgccgcgcg catcgaccag cagcacgcgc cccgactcga
     1861 gttcggcggc aagatccgcg gcgccgatcg cgcgatcggc ctgcagctgc gggacataga
     1921 ggcgcgggtc gcggcgacgt gcaatcgtct cgaccgccat gccggccgcc tgccaggccg
     1981 cgaagccgcc gttcaggacc gcgatggcct cgtggccgag ccagcgcagc atccaccagg
     2041 cgcgcgcggc atacgcgccc gggccggcat cgtaggcgat cacctgcacg ttcgcattga
     2101 tgccccagcg gccgaacgtg atcgccaggc gctcggggtc gggcagcggg tggcggcccg
     2161 tggtcggacc ggccggcccc gacaggtcgc gctcgaggtg cgcatactgt gcgcccggaa
     2221 tgtgtccctg ggcgtaggcc gcggcgccgg cgtccggatc ggcgagctcg aaacggcagt
     2281 cgagaatcac tacttcggga cgcccgagca gggcgcccag ctgcgcgacg tcgatcaggg
     2341 tcgaatacat gcgacaattc tcgactgaaa tcgtggccac tacaattaat cagtcatgat
     2401 cgacctgtac tggggcagtg gcagcccata ttgctggcgg gtactgctcg cgctcgagca
     2461 caagcggctc gagtacgtaa gccaccagtt gcagttctcc aggcaggagc acaaggcgcc
     2521 gcacatcgtc gcgatgaacc cgcgcggccg cctgccgatc ctgaagcacg atgactacgt
     2581 ggtcttcgag tccgtcgcgg tgctgcatta cctcgacctc aagtatcccg agccgccgat
     2641 cttcgggcgc acgcccgagg aggccggggt gatcgtgcgc gtgatctgcg agttcgaggc
     2701 atacgcgcag gagcacctgg cgaagatcac gcgcgcgctg ttcggagccg gggtcggcga
     2761 ggacaacgtg ggcgagctga cccgcgcgat gctggtggtg gcgagcgagg cgcgaacgat
     2821 cgaagggcgg ctctcgaagg gcgactggat cgtcggcgat gcctactccg cggcggacat
     2881 ggtgatctat ccggccatca tggtgctgct gcgcgcgctg cagcgaccgg gcgccgaggt
     2941 gctcgcttca cgcttcatgc ccgtcgcggt caactacccg tcgctcgcgc gctggctcga
     3001 tcgggtcgcg gccctgcccg gttacgagaa gacctacccg ccgcattggc tggccagcgc
     3061 gcggcacggt tgataccctg tcgcgctggt ttcctgcagg gtacgccgaa gaatggccga
     3121 caaggtttac atcgaattcc ccggacgcat cgtcctgatc ggcttcggca gcatcggcca
     3181 gggcgtgatg ccgctcatcc tgcggcacat cggcatcgac ccgagccgca tctcggtcgt
     3241 cacggccgac gaccgcggtc gcaaggaggc cgaggaatac ggcatcaagt tcgtcaaggc
     3301 gcccctgacg cgcgaaaact accgccgcat cctcgatgcc aacatcggcc gcggcgattt
     3361 cgtgctcaac gtctcggtcg acgtatcgag cgtcgcgctc gtgaagtact gctgggaacg
     3421 cggcgcgatg tacctcgata cctgcatcga gccgtgggcg ggcggctata cctcgccgac
     3481 catgaccccc gcgcagcgta ccaactacgc cctgcgcgag gccgcgctcg cgctgcgccc
     3541 cggcagcgat cgcgcgccga ccgcggtgat cacgcacggc gcgaacccgg gtctcgtctc
     3601 gcacttcgtc aagcaggcgc tgatcaacat cgccggcgac ctcggcgtcg aggcggcgcc
     3661 gcgcaaccgc gcggagtggg ccaagctggc gcgccagctc ggcgtgacca cgatccacat
     3721 cgccgagcgc gacacgcagg tgtcgagcca gccgaagaag gtgaacgagt tcgtcaacac
     3781 ctggtcgatc gacggcttcg ttggcgaggg ctgccagccg gccgagctcg gctggggcac
     3841 gcacgagcga aatttcccgc gtgacggcaa gcgccacgag ggcggctgcg gggcggcgat
     3901 ttacctgatg cagccgggcg cggccacgcg cgtgcgcacc tggacgccga atgccggcca
     3961 catcatgtcg ttcctgatca cgcacggcga gtcgatttcg atcgccgatt acttcaccgt
     4021 gatcgagagc atgcaaccgg tctaccgccc gaccgtgcat tacgcctatc acccgtcgga
     4081 cgcggcagtg ctgtcggtgt gggaactgca gggccgcaac tggcagctcc aggacaacac
     4141 ccgcatcatg atggacgaca tcgtgagcgg catggacgag ctcggcgtgc tgatcgccgg
     4201 ccacaagaag aatgcctact ggtacggctc gcagctcacc gtcgaggaag cgcgcaagct
     4261 ggcgccccac aaccaggcga ccagcctgca ggtcacgagc tcctgccttg ccggcatggt
     4321 ctgggcgatg gagaatccga actgcggcct gatggagccg gacgacctcg actacaagcg
     4381 cctgctcgag atctgcaggc cgtatctcgg caccnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnncacctc gtcatcgagc cgcttgccga gcagggcgcg cgcgagcggc
     4561 gcgtccatgc tgacgaagcc gggcgcggcg tcgaattcgt cggggccgac gatgcggacg
     4621 cgcagttcgc ggcgatcctc tccctcgagg gtgacccagg cgccgaaata gacacgctcg
     4681 cgatcatcgg gcggccgatc gaccaccacc atgccctcga gccgctggcg caggaagcgc
     4741 acgcggcgat cgatttcccg caggcggcgc ttgccgtagg tgtattccgc gttctccgag
     4801 cggtcgccct gtgcggcggc ctccgcgacc gcctgcgtga cctgcgggcg ctcgacgcgc
     4861 cagagctggt cgagctcggc cttcaggcgg gcgtgaccgg cgggggtaat gaagcgggat
     4921 ccgggcgtct ggggggggcg atatcggctc atggggccgg gattctcgcg tttcttgcgc
     4981 cagttctcac ctccgtgccg tgcagcgcgg ggggagtgtg gcggggctca cagtctgcgg
     5041 cgggggccgg gcatagccta tgaaccatgt ccgcacggta ttacacgcac ttcgtgaccc
     5101 cgggcgacga ggcccgggcg gcccatgagt ggagtggtgt ggtggaactg gggcgcgagc
     5161 tcgaccagcg tgccggtgcc ggcgaactgg cgcggctgct ggcgcagagc ttcgagctcg
     5221 agttcgagga catccgtatc ttgcactggg cgaggcttca ctagacctga atcccctggc
     5281 ggacttcctt cccttcattg aaagtccgca tactgcgacc ccggcttcaa acccggggtc
     5341 tttttttttc tggtgcgggc tggtcatgcg ctttctcaaa cccctcgcct ggacggctct
     5401 tgtcattgcg ttgctgatcg gcgcatatgc gtgggccggt tactggctgg cgccgcgcct
     5461 cgtgcagtcg aagctcccgc cggcgatcag cgcggccacc ggcctgcgcc tgaccctggg
     5521 cgaagtggcc atcaggccgt tctcgctgtc ggccgacctg cgggacgtgt cgctcaccga
     5581 gcccgacggc acggcattgc tcgcggcgca gcgtctctac atcgacgcgc ggctcgccgc
     5641 gctctggcgc cgcggcgtga tcctcgagtc gctcctgctc gagcagcccg ccctgaacct
     5701 catcctgcgc gaggacggca gcgtcaatct ggtcacggcc tttgaatcga aggccccgcc
     5761 gccgccgccc ggtgaggagg gcaggccgct ccctgtcagc atcgacgaca tccggatcca
     5821 gggcggcgtg atcgatgccg ccgacctgcg gcgcgccacg ccgctgcaac ggcgcctcga
     5881 gccgatcaat gtgcgcgtgc agaatttcac cacgagcgcc ggcgacgagg gcgacttcag
     5941 catcaccggt cgcgacgagg acggcggcga gatcacgctc aggggccagc tcgccgtgcg
     6001 accgttcgcg ctccacggcg acctcgaagt gaagggcctc agtgcgcgca cggcctggga
     6061 ggtcgccggg cgctacgggc gcgttgcgcc gccccagggg gtcatcgatt tcaggaccct
     6121 ctaccagatc gccgcggcag acgatggcat gcgcatcgcg ctcgatgcgc tcgatttcga
     6181 ggcgcgcgaa ctcgcggtgc gcccggcggg cggcgaggag gactgggtga aggtcgccgc
     6241 gctgtccgcc accggcgcga gcgtggacgt gcgcgaatcg ctcgtgaagc tgcccgacct
     6301 gcgcgtgcag ggcctcgatg tgagcgcctg gaccgaggcg gatggcggcg tcaacctggc
     6361 tgcactcgcg ccggccgagg cggcgccggc cgaacccgcg gcagctgtcg tgccggccga
     6421 gtccgccgcg cccgtagcat cgcccgagcc tgccgagccc gcgaagggct ggcgcatcga
     6481 ggcgccgatg ctgcgcgtca ccgctgcgcg tatcgcgttc gaggaccgca atgcgccgca
     6541 gcccgtgcgc tacgtgctcg cgccgctcga actcgacatc gacggctatg cgagcggcgc
     6601 gccggcagtc actgccgcgc tgcgctcggg attcaatgaa accggcaagc tcgcgatcga
     6661 ggggcagtgg cggctcgacg cttcaggcgg tgccttcacg gtcgccgccg agacgctgcc
     6721 cgtcgtgttc ctgcagccgt acctcgagcg gaccaccgac ctcgtgctga gaagcggcga
     6781 cgtctcggtc gagggcaagg tcgcgatcga tcgggccgcg gagggcaaac ccaggatcgg
     6841 cttcgagggc aacgcgaccc tggcgcgctt ccacagtgtc gatcgcgagc tgcgggagga
     6901 cttcgtcagg ttcaggcggc tcgccgcgag ccggatcagc tacagttccg cgccgaccac
     6961 gctgcgtgtg cgcgacctgc tggcgcgcgg cgcctacttc aagctggtca tctcgccgga
     7021 atcgaccacc aacgtcgccg aagtgctttc gccgccgcgg ctgcgcaagg ataccgccgc
     7081 cgagacggcg gccacggcgg acgccggcgc ggcaaagact gcgcgggccc cggcgaggaa
     7141 gctgcgcatc gacaaagtgc gtgtcgtcga cagcgcggca ttcttcgccg acctgtcgat
     7201 ccggccgagc ttctcgaccg gcatcgagga attcaatggc acgatcacgg ggctgtcgtc
     7261 cgatccggcc gcgcgcgccg acgtcgtgct cgacggcaag gtcgaccgct acgcgccggt
     7321 cgaagtgcgc ggcgaggtga acctgctctc gagcacgacc ttcaccgatc tcacggcgga
     7381 cttccacaac atcgaactgc cgaccttcac accctattcc ggcaagttca tgggttacca
     7441 gatcgagaag ggcaagctcg acgcgcattt ccgctatctg atcgaccatc gcaagctcga
     7501 tgcgcagcac aagttcaagc tcgaccagtt cacgctgggc gatcgcgtcg acagcgacga
     7561 cgcgatgaag atcccgatga agctcgccgt ggccttgctg aaggacaggc atggcgtgat
     7621 cgacatcgac ctgccggtct cgggctcgct cgacgatccg acattccgcg tcgggccgct
     7681 cgtctggaag gcgctgcgca acctgctcgt gaagatcatc acggcgccct ttgcgttgat
     7741 cggctcgctg ttcggtgccg gcgaagaggt gcgtttcgtc gatttcgcct atggcagtgc
     7801 gacgctcgat gcggccgcgg gggagcaact cgccaatgtg ggcaaggcgc tgaccgaccg
     7861 accgcagctg aagctcgggg tgccgctcgc gttcgatgcc gcccgcgatc gcgcggcact
     7921 cgtcgagcag cgcatgatcg aactgctggg cggcccggag gcgcatgcac tgcgcgaaac
     7981 cgataccggc gcctaccagg aagccctcga tcgcgcctgg cgcgaactga gcggcgagcg
     8041 gcgcgcgccg cgacccgagc gcgagaaaga cgaggcgaag gaagcccatg cgctgcgggc
     8101 gatcgaggcc ggcgagaccg cgttgcgcga acgcatcgtc attgcggatg ccgcgctggc
     8161 ggatctcgcg aagcagcgtg ccgacgccgt gcgcgaagcg ctcgtgaccg ggggtggact
     8221 cgatcccgaa cgaatcttcc tggtggcggg cgaacccgcg gccgatgcga cgagcggtgt
     8281 ccgcctcgcg ctccaggtgg aatgagccgt ttatactggg aaaatgcgta tcccattcct
     8341 gttcgccgcg gccgcgatcc tggccgcctg tgcggcgccg actccggcgc cggttcctga
     8401 gaccactgaa atcgccgttc ccgcgccgcc gcttgcgagc gggctcgtgc tcgacggcta
     8461 cgaccgcagt gtgcggccgc aggacgacct gcacgagttc gcgggcggca agtggctcgc
     8521 ggaaaccgcg attcccgccg atcgctcgag ctggggcact ttcgcgattc tcgacgagcg
     8581 cgccgagcga caggtgcgcg agatcgccga gcaggccgcg gcaactcccg tgcacgtgcc
     8641 gggctccgac ctgcagaaga tcggcgacct ctatgcgagc ttcatggacg agcaggccat
     8701 cgaggccgcg ggcctcgcgc cgctcgaggc ggatttcgcg cgcatccgcg cgctggcgac
     8761 gcatgccgac gtggtgcgct acatcggcga gtcgcagcgg cggctgcagc gcgtgccgct
     8821 cggctattac gtcgggcagg atgccggcga cgccacgcgc tacatcggca ccgtgcagca
     8881 ggccgggctg tcgatgcccg atcgcgacta ctacctggtg caggacaagc ggcaggcgga
     8941 gaatcgcaag cgtcatctcg cgtatgtcga gcagatgctc acgatggcgg gcgcgaagga
     9001 caccgccgcg cgcgcgcaac gcatccagca gctcgagacc cgcatcgccg cgatccagtg
     9061 gagcaaggtg cagaaccgcg atccgcgcgc gacctacaac cggacttcgc cgatcgaact
     9121 cgcgaagctc acgccggcat tcgactggac cgcgttcttc actgcggccg gcgcgccgat
     9181 cgaggcggtc gacatcaacc agccgaccta cgtcgaggcg ctggcgaagc tgatcgcggc
     9241 gacgccgctg ccgcagtggc gcgagtactt ttttttccag ctgctcgacg tgtcggcgcc
     9301 ttatctcgcc gcg
//
LOCUS       DAOQVY010000094         9273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_8304_length_9273_cov_5.191473, whole genome shotgun sequence.
ACCESSION   DAOQVY010000094 DAOQVY010000000
VERSION     DAOQVY010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8304_length_9273_cov_5.191473"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PKN91_09180"
     CDS             <1..493
                     /locus_tag="PKN91_09180"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNS28286.1"
                     /translation="DSRADRSLITSKVGAASGGIATASTSRGFGSGAGSLEGHSTTRM
                     SVPPGVPVGGRGEAASRSGASGKASRSREEIELVFDRNKGAIYALYSRALREQPELQG
                     KMVLEFTIAPSGEVTMCRVVSSEIGDPELERKIVARVRLFRFEARDVEAITTTKPIDF
                     FPA"
     gene            516..875
                     /locus_tag="PKN91_09185"
     CDS             516..875
                     /locus_tag="PKN91_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28287.1"
                     /translation="MNLQSEIQFFTELRVLDKARLLNAFLHELAEEARTTYGPGSEQV
                     TDTAHLRFVNELVHRVTRVLEQLLGEDRTRPADEVVLRMLLSPRTDKVAEKLVLNAYR
                     RAIHGFERYDTTVLMNT"
     gene            978..2126
                     /locus_tag="PKN91_09190"
     CDS             978..2126
                     /locus_tag="PKN91_09190"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28288.1"
                     /translation="MDLLANDYPGVILAERPGPCLSVYQPTHRAFPGRQQDPIRFRNL
                     VKSLGDSLRRNYPDRQVATLLAPFHALAGDTAFWNANLDGLAVLAAPDFFRCYSLQRT
                     VVERAIVADSFHTKPLMRIMQSADRYQILGLERHEARLFEGNRDSVDEIPLAAEVPRS
                     LDDVVDRDFERDRAIRTHGRVESGGMGRHGRSDVKRDAIDADTRRFFRQVDRAVFEQH
                     SRPSGLPLLLAALPEHHHLFREVSDNPQLLGDAIDVNPKALSNDELRARAWALVQPHY
                     LDRLARLVETFGTAVANRNGSADLAEVASAAVQGRVATLLLDADRELPGRIDATTGVA
                     TPGKPDDTQVDDLLDDLGEWVLRRGGEVVIVPTARMPTETGLAAIFRF"
     gene            complement(2207..3334)
                     /locus_tag="PKN91_09195"
     CDS             complement(2207..3334)
                     /locus_tag="PKN91_09195"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNS28289.1"
                     /translation="MKLEGLRVIDLSLFLPGPYLTQTMADHGAEVIKVEPPGEGEPNR
                     HIGAAEDGVSVFFRNTHRGKRSVTVNLKDAAQREALLTLAATADVFVEAFRPGVVDRL
                     GVGYAQVAARNPRIVYCSIAAFGQDGPYRDMPAHDLATEAMAGVVSLNLGEDGQPTIP
                     HVPAADMAASMFALSGILMALLRRERTGVGDYLDISMHDSILAWTPNVTGQVFTHKRP
                     PVVKHERSLGGSAFYRIYRTLDDRHVVLGGQEPKFIRNLLTELGRPEFIELCERGPGP
                     HQAPVVEFLRSVFATRTQAAWVDWFRGRDVCFAPVKNLREAFDDSHAIARGMRLVDPA
                     GREHIGTPIKFRNEPGQIDFTLPRQGEHTAFYSLRPPKAPR"
     gene            complement(3348..4571)
                     /locus_tag="PKN91_09200"
     CDS             complement(3348..4571)
                     /locus_tag="PKN91_09200"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNS28290.1"
                     /translation="MSAAGGTDAARASLPLAGIRVLTFENFGAGPFGSMYLADLGAEV
                     IKIENRDQGGDATRGMGPFFLGENDSHFFQTFNLNKRSVTLNLKTEAGQAVFRRLVAR
                     SDAVLNNLRGDQPEKLGLTYAALADVNPRIVCAHLSAYGRDNERKGWPGYDYLMQAEA
                     GYLHLTGEPGSPPARMGLSIVDYMTGITTAVALLASLLGAMKTGRGRDIDVSLFDVAL
                     HQLSYPGTWFINERHRTERMPRSSHPSATPVQLLRTQDGWIFVMCMTEKFWHELLAVL
                     GAQALGHDPRFATMQARREHRPELTAALDAIFETDTTSAWLARLQGRLPAAPVYNMEQ
                     ALDNPFVERIGMIQATPHPQKPGFRALANPIKLDGQRLHGRIGSALGADTRDVLREIG
                     YEDAEIDALASQRVI"
     gene            complement(4568..5713)
                     /locus_tag="PKN91_09205"
     CDS             complement(4568..5713)
                     /locus_tag="PKN91_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNS28291.1"
                     /translation="MATHDDGGMLESIERWVEREVRPIAKQYDHADEYPHELVEQMKE
                     LGLFGATISEEYGGLGLSASTYAQIVTAIAAVWMAPVGVFNSHLIMAACVQRHGTDEQ
                     KRRWLPRFASGELRGGIGLTEPNAGTDLQAIRTVARRDGGDYVIDGTKTWITNGIYGG
                     CFAVLVKTDPTAEPRHRGMSMFLCEKGPGFTIGKKLRKLGYKAVDSAELVFDGYRVPA
                     DQLIGGVEGKGFLHAVGGLGLGRINVAARGAGIALGSLREALRYAQERKTFGKPIAQH
                     QAIQLKLADMATRVEAAKLLIREAANKYDAGERCDLEAGMAKLFASEAGVENSLEAMR
                     IFGGYSYSIEFDIERFYRDAPLMCIGEGTNEMQRIIIARQLLERNPL"
     gene            complement(5713..6231)
                     /locus_tag="PKN91_09210"
     CDS             complement(5713..6231)
                     /locus_tag="PKN91_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNS28292.1"
                     /translation="MSQTAKQVGPGRYRETFGRYYEDFVVGDVYEHRPGRTITQTDNI
                     WFTLLTMNTHPLHFDEEYAKASEFGRCIVASPLTVGMLVGMSVSDVSQKAIANLGWRE
                     IRMTAPVFAGDTLYAESTVLEKRESKSRPGAGIVTVGTRGRNQDGTVVCEFERTILIA
                     RRGHGVEEGGDY"
     gene            6319..7086
                     /locus_tag="PKN91_09215"
     CDS             6319..7086
                     /locus_tag="PKN91_09215"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNS28293.1"
                     /translation="MAKALNGGQPRYRQLAEALIGDISRGRLRVGETLPGELELVERF
                     KVSRHTVREALRMLDDLGLIDRQQGVGTVVRARQSSPSYVQSVRTPSELMRYPADSRL
                     AVVASGIVKANRRLARLLDVRTGSEWLKVSALRRMKSASLPLGWTDVYLLPEFADIVP
                     QIGKRGLMVYQLIEAIHGEHVERIQVQLRAGLVTAELAGPLEATEGAPSLTVMRHYFG
                     RGGRPFEISVSEHPSDRYMYALDLQRGWQSSDTWGAG"
     gene            7083..7982
                     /locus_tag="PKN91_09220"
     CDS             7083..7982
                     /locus_tag="PKN91_09220"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HNS28294.1"
                     /translation="MSARAPLGLRSYLFAPANHARRAEKVFAAGADAAILDLEDAVAV
                     AEKAAARPLLVAAMQRPRNARGYARVNGVDSPFWQDDLAVVVGPWLDGVVLPKAESAA
                     QVRSFVAHLEDCERQARLAPGTLDLMLIVETALGVVNVEAIAAASPRIGRIALGGGDY
                     TSDLDLEWTAEEEALAYARARIAHASRAAGIEPPVDTVVIEVRDQARFRRSALNGRRL
                     GFLGKLCIHPDQVAPCHEVFTPGPAEIARARAIVAAFRDAEARGVASIQVDGVFVDYP
                     VVYKAQRVLALAERLAPAVTGDG"
     gene            complement(7966..8889)
                     /locus_tag="PKN91_09225"
     CDS             complement(7966..8889)
                     /locus_tag="PKN91_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNS28295.1"
                     /translation="MSAPGATARYRLLGALAAAGGAMLFASKGIIAKFAYARDIPFEV
                     LVVVRALLALPLFWAFALHREPARVIRSTRWQTIAMAAFAGIVCYYGGALLDFHALTF
                     VDASVERVLIFSYPAMVVAIDSAMRRSRPSAGVLTAVALTWIGIYFAVGGGDAGTLRA
                     NLYGSALVLAAALSYAIYFMIGERCTREIGASRYTLVAMSAAAAVLLVHALARGTLPR
                     LAAVDLQGWLLLAMLAVLCMFVPALLQAEGVRRLGAQRGALVSTVGPPTTIVLAWLLF
                     GERMSWLQLAGVALIVCGILVLDLAVFTRRR"
     gene            complement(8886..>9273)
                     /locus_tag="PKN91_09230"
     CDS             complement(8886..>9273)
                     /locus_tag="PKN91_09230"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNS28296.1"
                     /translation="WLEATRNCAGNPEHEIVAELAPEPGELVLNKTTIGAFASTGLEA
                     ALRGMGITEIVVTGVSTNNCVGMTAMEAADRQFGVVLVDDATGTCSDEMQAATVKTFR
                     RLWGRVATTDEIIAELAVARRAGARA"
BASE COUNT         1436 a         3140 c         3186 g         1511 t
ORIGIN      
        1 cgacagccgc gcagatcgct cgctgatcac ctcgaaggta ggcgccgcga gcggcggcat
       61 cgccacggcg agcaccagcc gcggcttcgg ttcgggcgca ggcagcctcg agggccacag
      121 tacgacccgc atgagtgtcc ctccgggcgt cccggtcggc gggcgcggcg aggcggccag
      181 ccgctcgggc gcgagcggca aggcgtcgcg cagccgggaa gagatcgaac tcgtgttcga
      241 ccgcaacaag ggcgccattt acgccctcta cagccgtgcc cttcgcgaac aacccgagtt
      301 gcagggtaag atggtgctgg aattcaccat tgcgccttcc ggcgaggtga cgatgtgccg
      361 cgtggtgtcg agcgagatcg gcgatccgga actcgagcgc aagatcgtgg cgcgcgtgcg
      421 gctcttccgc ttcgaggctc gcgacgtcga ggcgatcacc accaccaagc ccatcgactt
      481 cttccccgcc tgaaggaggc gcgcgcgagg ccagcgtgaa cctgcagtcc gagatccagt
      541 tcttcacgga gttgcgcgtg ctggacaagg cacggctcct gaacgccttc ctgcacgagc
      601 tcgccgagga ggcgcgcacc acctacgggc cgggtagcga gcaggtcacg gacacggccc
      661 acctgcgttt cgtcaacgag ctggtgcacc gcgtgacgcg cgtgctcgag cagctgctgg
      721 gcgaggatcg cacgcgcccg gccgatgagg tggtgctgcg catgctgctg tcaccgcgca
      781 ccgacaaggt ggccgaaaag ctggtgctga acgcgtaccg gcgcgcaatc cacggcttcg
      841 agcgctacga caccaccgtg ctgatgaata catagccgcg cggcagcttt cgaaaaagca
      901 tggaatggcc gacaatgcca agcgtgtgtg caggtcgcgc ctgacaccgc atgtattctc
      961 gagcgaggaa tgcaaccgtg gatctgctgg cgaatgacta tccgggcgtg attctcgccg
     1021 agcggccagg cccctgcctg tccgtttatc aaccgacaca tcgcgcgttt cccgggaggc
     1081 agcaggaccc gatccgtttt cgcaatctgg tcaagtcgct cggcgactcg ctgcgacgga
     1141 attacccgga tcggcaggtc gcaaccctgc ttgctccatt ccatgcgctg gcgggggaca
     1201 cggcgttctg gaacgccaac ctcgacgggc tcgcggtact cgcggcgccg gacttctttc
     1261 gctgctacag cctgcagcgc acggtcgtcg agcgggcgat cgtcgccgac agctttcaca
     1321 ccaagccgct catgcggatc atgcagtcgg ccgatcgcta ccagatcctc ggcctcgagc
     1381 gccacgaggc gcggttgttc gagggcaatc gcgacagcgt ggatgagatc ccgttggcgg
     1441 cggaggtgcc gcgcagcctc gacgatgtcg tcgatcggga tttcgaacgc gaccgcgcca
     1501 tccgtaccca cggcagggtc gagtctggcg gcatggggcg ccatggtcgc agcgatgtga
     1561 aacgcgatgc catcgacgcc gatacccggc ggttcttccg ccaggtggat cgtgcagtat
     1621 tcgagcaaca ttcgcgtccg tccggtttgc cgctgctgct ggcggcactg cccgaacacc
     1681 accatctgtt tcgcgaggtc agcgacaatc cgcaactcct cggcgatgcc atcgacgtca
     1741 acccgaaagc attgtcgaat gacgagctgc gcgcgcgcgc gtgggcgctg gtgcagccac
     1801 actatctcga tcgtctcgcc cgcttggtcg agacgtttgg caccgcggtg gcgaaccgta
     1861 atggcagcgc cgatctggcc gaggtcgcga gtgccgcggt gcaggggcga gttgcgaccc
     1921 tgctgctcga cgccgaccgc gagcttcccg gtcgcatcga tgcgaccacc ggcgtggcta
     1981 cgcccggcaa gccggacgat acgcaagtcg acgatttgct cgatgatctg ggtgaatggg
     2041 tgctacgccg gggcggcgaa gtggttatcg tgcctacggc gcgcatgccg accgaaaccg
     2101 gattggccgc gatcttccga ttctgatctt gcggtgcgat cggcatgccg ctgccggtcg
     2161 aatgcccggc ccatgctgcc ggagtctgcc ggcgatacgc atgtgatcag cgtggtgcct
     2221 ttggtggcct gagagaataa aacgccgtgt gctcgccctg gcgcggcagc gtgaaatcga
     2281 tctgccccgg ctcgttcctg aacttgatcg gcgtgccgat gtgttcccgg cccgccgggt
     2341 cgacgaggcg catgccgcgc gcaatcgcgt ggctgtcgtc gaacgcctcg cgcaggttct
     2401 tcaccggcgc gaagcacacg tcccggccgc gaaaccagtc gacccacgcg gcctgcgtgc
     2461 gcgtcgcgaa cacgctgcgc aggaactcca ccaccggtgc ctggtgcggc cccgggccgc
     2521 gctcgcacaa ctcgatgaac tccggcctgc cgagctcggt gagcaggttg cggatgaact
     2581 tcggctcttg cccgccgagc accacgtgcc gatcgtcgag cgtgcgatag atgcggtaga
     2641 aggcgctgcc gccgagactg cgctcgtgct tcacgaccgg ggggcgcttg tgcgtgaaca
     2701 cctggccggt cacgttcggt gtccaggcga ggatcgaatc gtgcatcgag atgtcgagat
     2761 agtcgccgac accggtgcgc tcccgccgca gcagcgccat caggatgccg gacagggcga
     2821 acatcgaggc cgccatgtcc gcagccggca cgtgcgggat cgtcggctgg ccgtcctcgc
     2881 cgaggttgag gctcacgacg ccggccatcg cctcggttgc gagatcgtgc gccggcatgt
     2941 cgcggtacgg tccgtcctgg ccgaatgccg cgatcgagca atagacgatg cgcgggttgc
     3001 gggcggcgac ctgcgcgtag ccgacgccga gccggtcgac gacaccgggc cggaaggctt
     3061 cgacgaacac gtccgcggtg gcggcgagcg tgagcagcgc ctcgcgctgc gcggcatcct
     3121 tcagattgac ggtgacgctg cgcttgccgc gatgcgtgtt gcgaaagaag acgctgacgc
     3181 catcctcggc cgcgccgata tgccggttcg gttcgccctc gccgggcggc tccaccttga
     3241 tcacctcggc gccgtggtcc gccatcgtct gcgtgaggta aggcccgggc aggaacagcg
     3301 acaggtcgat gacgcgcagg ccctcgagct tcatcggcgg cgccccctca gatgacccgc
     3361 tgcgaggcga gcgcgtcgat ttcggcgtcc tcatagccga tctcgcgcag cacgtcgcgc
     3421 gtgtcggcgc cgagcgccga gccgatgcgg ccgtgcaggc gctgcccgtc gagcttgatg
     3481 gggttcgcga gggcccgaaa gcccggtttc tgcgggtggg gcgtcgcctg gatcatgccg
     3541 atgcgctcga cgaacgggtt gtcgagcgcc tgctccatgt tgtacacggg cgcggccggc
     3601 aggcggccct gcagcctcgc gagccaggcc gaggtcgtat cggtctcgaa tatggcatcg
     3661 agcgccgcgg tcagctcggg acgatgctcg cgccgcgctt gcatagtcgc gaagcgcgga
     3721 tcgtggccga gcgcctgcgc gccgagcacc gcgagcagct cgtgccagaa cttttccgtc
     3781 atgcacatga cgaatatcca cccgtcctgc gtgcgcagca gctgcaccgg cgtggcggac
     3841 gggtgcgagc tgcgcggcat gcgctcggtc cggtggcgct cgttgatgaa ccaggtgccg
     3901 gggtaggaaa gctggtgcag ggccacgtcg aacaggctga cgtcgatgtc gcggccgcgg
     3961 cccgtcttca tcgccccgag caggctcgcg agcaacgcca ccgcggtggt gatgccggtc
     4021 atgtaatcaa cgatcgacag gcccatgcgc gcgggcggcg agccgggctc gccggtgagg
     4081 tgcaggtagc cggcctccgc ctgcatcagg tagtcatagc ccggccaccc cttgcgctcg
     4141 ttgtcgcgcc cataggcgga caggtgcgcg cagacgatgc gcgggttgac atcggcgagc
     4201 gccgcgtagg tgaggcccag cttctcgggc tggtcgccgc gcaggttgtt cagcaccgca
     4261 tcggagcgcg ccaccagccg gcggaacacg gcctggccgg cctcggtctt caggttcagc
     4321 gttacgctgc gcttgttcag gttgaaggtc tggaagaaat ggctgtcgtt ctcgccgagg
     4381 aagaacggcc ccatgccgcg tgtcgcatcg ccgccctggt cgcggttctc gatcttgatg
     4441 acctctgcac caaggtcggc gaggtacatc gagccgaagg ggcctgcgcc gaagttctcg
     4501 aaggtcagta cgcgaattcc ggcgagcggc agcgaggcgc gcgcggcatc agtcccgcct
     4561 gcggcgctca cagcgggttc ctctcgagca gctggcgcgc aatgatgatg cgctgcattt
     4621 cgttcgtgcc ctcgccgatg cacatcagcg gcgcatcgcg atagaagcgc tcgatgtcga
     4681 attcgatcga gtagctgtag ccgccgaata tacgcatcgc ctcgaggctg ttctcgacgc
     4741 ctgcctccga ggcgaagagc ttggccatgc cggcctccag gtcgcagcgc tcgcccgcat
     4801 cgtacttgtt cgcggcctcg cggatcagca gtttcgccgc ctcgacgcgc gttgccatgt
     4861 cggcgagctt cagctggatc gcctggtgct gcgcgatcgg cttgccgaag gtcttgcgtt
     4921 cctgcgcata gcgcagtgct tcgcgcagcg agccgagcgc gatgccggcg ccgcgcgccg
     4981 cgacgttgat gcggccgagt ccgaggccgc cgacggcgtg caggaagccc ttgccctcga
     5041 cgccgccgat cagctggtcg gccggcacgc ggtagccgtc gaacaccagt tcggcgctgt
     5101 cgaccgcctt gtagccgagc ttcctgagct tcttgccgat cgtgaagccg gggcccttct
     5161 cgcacaggaa catgctcatg ccccggtggc gcggttcggc ggtcggatcg gtcttgacca
     5221 gcacggcgaa acagccgccg tagatgccgt tcgtgatcca ggtcttcgtg ccgtcgatca
     5281 cgtagtcgcc gccgtcacgg cgtgcgacgg tacggatcgc ctggaggtcg gtgccggcat
     5341 tcggctccgt gaggccgatg ccgccgcgca gctcgccgct cgcgaagcgc ggcagccagc
     5401 gccgcttctg ctcgtcggtg ccgtggcgct gcacgcacgc ggccattatc aggtgggagt
     5461 tgaagacgcc gaccggcgcc atccagactg cggcgatcgc cgtgacgatc tgcgcgtagg
     5521 tcgaggccga caggccgagg ccgccatact cttcgctgat ggtcgcgccg aagaggccca
     5581 gttccttcat ctgctcgacc agttcgtgcg gatactcgtc cgcgtggtcg tactgcttcg
     5641 cgatcggccg cacctcgcgc tcgacccagc gttcgatcga ttcgagcatg ccgccgtcgt
     5701 cgtgggtcgc catcagtagt caccgccttc ctcgacgccg tggccgcgcc tggcgatgag
     5761 gatcgtgcgc tcaaattcgc acacgaccgt gccgtcctgg ttgcgacccc tggtgcccac
     5821 cgtgacgatg ccggcgccag ggcgcgactt cgactcgcgc ttctcgagca cggtcgactc
     5881 ggcgtacagc gtgtcgccgg cgaacactgg cgcggtcatg cggatctcgc gccagccgag
     5941 gttcgcgatc gccttctggc tcacgtcgga cacgctcatg cccaccagca tccccacggt
     6001 caggggactc gcgacgatgc agcggccgaa ttcgctcgcc ttggcgtatt cctcgtcgaa
     6061 atgtagcgga tgcgtgttca tcgtcagcag cgtgaaccag atgttgtcgg tctgggtgat
     6121 cgtgcgtccc gggcgatgtt catagacgtc gccgacgacg aaatcctcgt agtagcggcc
     6181 gaaggtctcg cggtaacggc cggggccgac ctgcttggcg gtctgggaca tgggaggcct
     6241 gcaaaacggg caaatgtccg gacaaatcta ggtctggggg ccgtgactgt caaccgccgc
     6301 cggttgtaca gtgatgccat ggcaaaagca ctcaacggcg gccagccccg ttaccggcag
     6361 ctggccgaag ccctcatcgg cgatatcagc cgcggcaggc tcagggtcgg cgagaccctg
     6421 cccggcgagc tcgagctggt cgagcgcttc aaggtcagcc gccacaccgt gcgcgaggcg
     6481 ctgcgcatgc tcgacgacct cgggctgatc gaccgccagc agggggtcgg caccgtggtg
     6541 cgcgcgcgcc agtcctcccc gtcctacgtg cagtcggtgc gcacgccctc cgagctgatg
     6601 cgctacccgg ccgacagccg cctggccgtc gtggcgagcg ggatcgtcaa ggcgaaccgg
     6661 cgcctcgcgc gactgctcga cgttcgcacc ggctccgagt ggctcaaggt gagcgcgctg
     6721 cgccgcatga agtcggcgtc gctgccgctc ggctggaccg acgtctacct gttgccggag
     6781 tttgccgaca tcgtgccgca gatcggcaag cgcggcctca tggtgtacca gctcatcgag
     6841 gcgatccatg gcgaacacgt cgagcgcatc caggtgcagt tgcgtgccgg cctcgtcacg
     6901 gccgagcttg ccggtccgct cgaggctacc gagggcgcgc catcattgac cgtcatgcgc
     6961 cattatttcg ggcgcggcgg gcgaccgttc gagatttcgg tctccgagca tcccagcgac
     7021 cggtacatgt atgcgctcga cctgcagcgc ggctggcaat cgtccgacac ctggggtgcc
     7081 ggatgagcgc ccgcgcgccg ttgggcctgc gcagttacct gttcgcgcca gccaatcacg
     7141 cgcgccgcgc ggagaaggtg ttcgccgccg gcgctgatgc cgcgatcctc gacctcgagg
     7201 atgcggtcgc cgtggcggag aaggccgctg cgcgaccgct gctggtggcc gcgatgcagc
     7261 ggccccgcaa cgcgcggggc tacgcgcggg tcaatggcgt cgactcgccg ttctggcagg
     7321 acgatctcgc tgtggtggtc gggccgtggc tcgacggcgt cgtattgccg aaagccgagt
     7381 ccgccgcgca ggtgcgctcg ttcgtcgcgc accttgagga ttgcgagcgg caggcgcgac
     7441 tcgcgccggg caccctcgac ctgatgctga tcgtcgagac cgcgctcggc gtcgtcaacg
     7501 tcgaggcgat cgctgccgct tcgccgcgca tcggccgtat cgcgctcggc ggaggcgact
     7561 acaccagcga cctcgatctc gagtggacag cggaggagga ggcgctcgcc tacgcgcgcg
     7621 cgcgcatcgc gcacgcctcg cgggccgcgg gcatcgaacc gccggtcgat acggtcgtga
     7681 tcgaagtgcg cgaccaggcg cgctttcgca ggtcggcgct caacggccgg cgcctcggct
     7741 tcctgggcaa gctctgcatc cacccggacc aggtcgcgcc ttgccacgag gtcttcacgc
     7801 cggggcccgc cgaaatcgcg cgggcgcgcg ccatcgtggc tgcgttcagg gacgccgagg
     7861 cacgcggcgt cgcctcgatc caggtcgatg gtgtgtttgt cgactatccg gtcgtgtaca
     7921 aggcgcagcg cgtgctcgcc ctcgcggaac ggctcgcgcc cgcagtcacc ggcgacgggt
     7981 gaagacggcg agatcgagga ccagtattcc gcagacgatc agcgccacgc cggccagctg
     8041 caaccacgac atccgttcgc cgaacagcag ccacgccagc acgatcgtgg tcggcgggcc
     8101 cactgtgctg accagggcgc cgcgctgcgc cccgaggcgg cgcacgccct cggcctggag
     8161 cagcgccggc acgaacatgc acagcacggc gagcatcgcg agcagcagcc agccttgcag
     8221 gtcgacggca gcgagccgcg gcagcgtgcc acgcgcgagc gcgtgcacga gcagcacggc
     8281 ggccgccgcg ctcatcgcaa ccagcgtgta gcggctggcg ccgatctcgc gcgtgcatcg
     8341 ctcgccgatc atgaaataga tcgcgtacga gagcgcggcg gcgagtacca gcgccgagcc
     8401 gtacaggttc gcccgcagcg tgcccgcgtc tccgccgccc acagcgaaat aaatgccgat
     8461 ccacgtgagc gcgaccgcgg tcagcacgcc ggcggagggc cgactgcgcc gcatggccga
     8521 gtcgatcgcg accaccatgg ccggatagct gaagatcagc acccgctcga cgctggcatc
     8581 gacgaacgtg agcgcgtgga agtcgagcag cgcgccgccg tagtagcaga cgatgccggc
     8641 gaaggcggcc atggcgatcg tctgccagcg tgtcgacctg atgacgcgcg ccggctcgcg
     8701 gtgcagcgcg aatgcccaga acagcggcag tgcgagcagt gcccgcacca cgaccagcac
     8761 ctcgaacggg atgtcgcgcg cataggcgaa ctttgcgatg atgcccttgc tcgcaaacag
     8821 catcgcgccc cccgctgccg cgagtgcgcc gagcaaccgg tagcgtgcgg tggcccccgg
     8881 tgcgctcaag cccgcgcgcc cgcgcgacgc gcgacggcga gctcggcgat gatttcgtcg
     8941 gtggtcgcga cgcggcccca cagccgcctg aaggtcttga cggtcgcggc ctgcatctcg
     9001 tcgctgcagg tgccggtggc gtcgtcgacc agcacgacgc cgaactgccg gtcggccgcc
     9061 tccattgcgg tcatgccgac gcagttgttg gtcgatacgc cggtgacgac gatctccgtg
     9121 atgcccatgc cgcgcagggc cgcctcgagg ccggtggagg cgaaggcccc gatcgtcgtc
     9181 ttgttgagca ccagctcgcc cggttcgggc gcgagttcag cgacgatctc gtgctcggga
     9241 ttgcccgcgc agttgcgggt ggcctcgagc cat
//
LOCUS       DAOQVY010000095         9236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_8350_length_9236_cov_4.485023, whole genome shotgun sequence.
ACCESSION   DAOQVY010000095 DAOQVY010000000
VERSION     DAOQVY010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9236
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8350_length_9236_cov_4.485023"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PKN91_09235"
     CDS             <1..509
                     /locus_tag="PKN91_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28297.1"
                     /translation="IVPAATLNLGSAALASREMLLPAARRLGADAVLVGRGDGGAASI
                     WQWSLASTEVAESWSGAPAAGVHGAADAFVRTAVVSAVATGDAEVRVSVSGVGDLAAY
                     AGVSQALSRVAGVRQVALEEAAGNTAIFRVTMRGGTDALGAALAGHGRLVPAATPTPG
                     VLALTWLP"
     gene            515..1069
                     /locus_tag="PKN91_09240"
     CDS             515..1069
                     /locus_tag="PKN91_09240"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNS28298.1"
                     /translation="MRHLPNIICLARIVLTWPILVCLARDRADLALALFTVAAVSDGL
                     DGWLAKRNGWTTNMGRLLDPLADKILLVFVFVFATWLGLVPMWLTAAAVARDVMIGLG
                     ASIYRVWFGPLNGRPTIISKINTLLQIIVLVLAMLRAATGVPPWEVILALSAVTLVTT
                     LVSAWDYVGRFARRAWQLAATPHA"
     gene            1062..1730
                     /gene="hda"
                     /locus_tag="PKN91_09245"
                     /pseudo
     CDS             1062..1730
                     /gene="hda"
                     /locus_tag="PKN91_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
     gene            complement(1745..2347)
                     /gene="wrbA"
                     /locus_tag="PKN91_09250"
     CDS             complement(1745..2347)
                     /gene="wrbA"
                     /locus_tag="PKN91_09250"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HNS28299.1"
                     /translation="MNEILVLYYSRTGATAELARQVCRGIEAVAGMGARLRTVPPVSA
                     ESEAPAKPVPASGPPYATLADLRETRGLVLGSPTRFGNMAAPLKYFLDGTSSLWLDGT
                     LAGKPAGVFTSTQTQHGGQETTLLSMMLPLLHHGMYIVGIPFTERELSTTRTGGSPYG
                     ASHVAAHATGPTLSEEEKTLAQVLGRRVAELAARIASPGR"
     gene            2372..2647
                     /locus_tag="PKN91_09255"
     CDS             2372..2647
                     /locus_tag="PKN91_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNS28300.1"
                     /translation="MVARKCLVSGRVQGVFYRATVARRARELAILGHARNLPDGRVEV
                     LACGAPEAVTHFIQALWAGSTASRVTAVEIEELDLAPDQRPRGFETG"
     gene            complement(2617..3315)
                     /locus_tag="PKN91_09260"
     CDS             complement(2617..3315)
                     /locus_tag="PKN91_09260"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNS28301.1"
                     /translation="MARWISSARDSRRAEPRARRAGLAAATLAAWALASAAQSAPADG
                     QQEEAGLRSVIERAIAQAECFPDEYESAVWFTMMEPRLVKRVPDKAERMEILRTVFCE
                     AHRKGEVRLPPGLVMAVIDIESRFDRWAVSYAGAVGLMQIMPFWPEKLGMQRHQLTQI
                     GPNIRMGCAILRYYLRYESNNIQKALARYNGSVGRRTYSDLVINSWSRWNGADDLGLS
                     ASVSAQPVSKPLGR"
     gene            complement(3272..4984)
                     /locus_tag="PKN91_09265"
     CDS             complement(3272..4984)
                     /locus_tag="PKN91_09265"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNS28302.1"
                     /translation="MRWSRYPINTMKDTPAEAELVSHRLMLRAGLIRRLAAGIYTWTP
                     LGLLTLRKVETIVREEMDRAGAFELLMPAVQPAELWQESGRWGEYGPELLRLKDRHER
                     DFVIGPTHEEVITDFVRRELQSYRQLPVNFYQIQTKFRDEIRPRFGVMRGREFIMKDA
                     YSFHLDEASMAEGYAAMREAYTRMFTRMGLEFRAVRADSGAIGGSASEEFQVLADSGE
                     DAIVFSDGDAYAANLEMAEAVAPAAPRPPATAALARVATPEVRTIAALSGFLGVPATQ
                     CGKTLLVDGADGGVVALVLRGDHELNAVKAQKLAGVASPLRMASAEAVLAASGAEPGF
                     VGPVGLKCPIIADRSAAVMADFVCGANAHDTHLTGVNWGRDLPEPQVADIRNVVEGDP
                     SPTGTGRLAIVRGIEVGHIFQLGRKYSEAMNCAVLDEGGRSTTVLMGCYGIGVTRVVA
                     AAIEQNHDDRGIIWPEPIAPFQVVLVPINLAKSPRVRETADRLYEEFTRAGIEVLLDD
                     RDARPGAKFADAELLGIPHRIVVGERGLDAGKLEYRHRRATDSEDMALDGALDFLRSR
                     LAPR"
     gene            5047..5799
                     /gene="pssA"
                     /locus_tag="PKN91_09270"
     CDS             5047..5799
                     /gene="pssA"
                     /locus_tag="PKN91_09270"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNS28303.1"
                     /translation="MSTLSPDSAPRSRGVYLLPNAFTTGCLFAGFYAIVAAIDGNFDR
                     AGLAVFAAMLFDGLDGRVARWTGTESAFGKEYDSLADMVAFGLAPAIVMYQWGVERIA
                     EYGHTWGRVGWVACFFYAVTAALRLARFNTRAAEDKRYFEGLPSPSAAAVVAAYVWFT
                     STWRGTDALVELVAAFCVTMLAGALMVSRFPYMSLKQVDVDKRVRYYYFVVVPLFLAL
                     IAIDPPTMLLAMFSTYALSAPAQWVVRRLRRL"
     gene            5895..6497
                     /locus_tag="PKN91_09275"
     CDS             5895..6497
                     /locus_tag="PKN91_09275"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNS28304.1"
                     /translation="MAVPATPREPGPAGEWEALRAEVAACTKCSLCETRTQTVFGVGD
                     RRARCLIVGEAPGAEEDRRGEPFVGRAGQLLDSMLRAVRLSRDTVFIANVLKCRPPGN
                     RDPRPDEAASCLPYLERQIALIEPAVMVAVGRVPAQVLLGSDAAVARLRGRVHQFGER
                     AIPLVVTYHPAYLLRAPAEKRKAWDDLKLARDLLDGRGAR"
     gene            6478..6963
                     /gene="rimI"
                     /locus_tag="PKN91_09280"
     CDS             6478..6963
                     /gene="rimI"
                     /locus_tag="PKN91_09280"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNS28305.1"
                     /translation="MAAERDDLPVPEVQLRPMTEADVDRVAALERESYPFPWSEGIFR
                     DCLRVGYVCRVVELGPALIGYGVMSHGAGEAHILNLCVREAMRGRGIGRSLLRHLLQQ
                     AAGAGMDEAFLEVRPSNLAAIRLYQSMGFEPVGVRRSYYQAVGGREDATVLRLDLRGL
                     R"
     gene            complement(6960..7436)
                     /locus_tag="PKN91_09285"
     CDS             complement(6960..7436)
                     /locus_tag="PKN91_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNS28306.1"
                     /translation="MPQSGLDRTDLRILDVLQQDGSLSAAELAERVNLAVTTCWRRVR
                     RLEEQGVIRQRVAILDREKVGFGVTVFAHVKLVAHGRDNLARFDQAIRRHPEVLECYT
                     LMGDWDFLLRIVARDIKAYEAFFLDQLSKIPGIQSLSSSIAMTVVKETTQLPLALR"
     gene            7590..8801
                     /locus_tag="PKN91_09290"
     CDS             7590..8801
                     /locus_tag="PKN91_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNS28307.1"
                     /translation="MPQPPARPGDKADFSYLKLQPAGKQPRPDVKVSAQATLPLATGI
                     VRVLDDEHRAVGAWDPHLEPEVLQMGLRHMLLTRIFDERMQRTQRQGRISFYMRSHGE
                     EAVSVAQTMALRPGDMLFPSYRNQGIYMVRGRPLVDLMCQLLSNTRDMCKGRQLPVMY
                     HWREGNVFSISGNLATQFPQAVGWAMAAAIKGEDHVAATWIGEGSSAESDFHAGMVFA
                     EVYQAPVLLNIVNNQWAISTFQGFAGGEKSTFAARGPGYGVAGIRVDGNDFLAVHAVT
                     QWAAERARRGGGPTLIEHVTYRASAHSTSDDPSRYRPKDEYERWPLGDPVERLKQHLI
                     ALGEWSEERHTALAQELDEQVSAAWREATRYGTLNEGPRLDANLMFEDVFKEMPEHLV
                     RQREQLRAERG"
     gene            8812..>9236
                     /locus_tag="PKN91_09295"
     CDS             8812..>9236
                     /locus_tag="PKN91_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNS28308.1"
                     /translation="MNMIQALNSAMDVMLGRDPGVVVLGEDVGYFGGVFRTTDGLQRK
                     YGEHRVLDTPISELGIVGTSIGMAVNGLRPVAEIQFADYIYPGYDQIVSELARLRYRS
                     AGEFFAPVTIRTPCGGGIRGGQTHSQSPEAVFAQICGIKV"
BASE COUNT         1301 a         3192 c         3240 g         1503 t
ORIGIN      
        1 cgatcgtgcc ggcggcgacc ctgaacctcg gatcggctgc gctcgcatcg cgcgagatgc
       61 tgctgcccgc cgctaggcgg ctcggtgccg atgccgtcct ggtcgggcgc ggcgacggtg
      121 gcgcggcgtc gatctggcaa tggtcgctcg cgtccaccga agtggccgag agctggtcgg
      181 gcgcgccggc cgcaggggtg cacggcgctg ccgatgcctt cgtgcgcacc gccgtcgtgt
      241 cggctgtcgc cacaggcgac gccgaggtgc gggtcagcgt cagcggcgtc ggcgacctgg
      301 cggcctatgc gggggtctcg caggcactct cgcgagtcgc cggggtgcgg caggtcgcgc
      361 tggaggaggc ggccggcaac accgcgatat tccgagtcac gatgcgcggc ggcaccgatg
      421 cgctcggtgc ggcgctcgcg ggccatgggc gcctcgtgcc cgccgcgacc ccgacgcccg
      481 gcgtgctcgc actcacctgg ctgccctgaa ccccatgcgg cacctgccga acatcatctg
      541 cctcgcgcgc atcgtgctca cctggccgat cctcgtctgc ctggcgcgcg atcgcgcgga
      601 tctggcgctc gcgctgttca ccgtcgccgc cgtcagcgac gggctcgacg gctggctcgc
      661 caagcgcaac ggctggacca cgaatatggg gcgcctgctc gatccgctgg cggacaagat
      721 cctgctggtc ttcgtcttcg tgttcgcgac ctggctcggc ctcgtgccga tgtggctcac
      781 cgccgccgcg gtggcgcgcg acgtgatgat cggtctcggt gcgagcatct accgcgtgtg
      841 gttcgggccg ctgaacggcc ggcctaccat catcagcaag atcaacacgc tgctgcagat
      901 catcgtgctc gtgctcgcga tgctgcgcgc cgccaccggc gtgcctcctt gggaggtcat
      961 cctcgcgctg tcggccgtga ctctggtgac cacgctggtg tccgcttggg actatgtggg
     1021 ccgcttcgcc cggcgcgcct ggcagctcgc cgccacgccg catgcctgaa ccgcgccagc
     1081 tgccgctcgg cgtgcgcctg cgggaccgcg cgagcttcgc gagcttcgtc gccggggcga
     1141 atgcggaagc cgtgcgatac gtgacgcaac tcgcggccgg cgaaggtcct gccatcgcct
     1201 ggctgcatgg cccgactggc gccggcaaga cgcacctgct gcaggcctgc tgcgcgcacg
     1261 ctgcaggaca tgcccgcgcg ggttatgtcc cgctcaccga attcgagcgt ggggaccccg
     1321 cggccctcga cggttggcat ggcctcgacc tggtctgcgt cgacgacctg catctcgtcg
     1381 ccggccgacg cccgtgggac gaggcgctgt ttcgcctcta ccgtgacatc gaggagcgcg
     1441 gcgcgcgcct gctggtggcg gcgagcgagc cgccggcggc actcggctgg tcgctgcccg
     1501 acatcgcctc gcggttcgcg gcggcgccgg tgttcgcgct gcagcccctc gatgaagccg
     1561 ggcgcatgca ggcgctcgcg ctgcgcgccc gcttgcgcgg cgtggatctg ccggatgaca
     1621 ccgtgcgctt cctgcagcgg cgctttccgc gcgacatgca gacgctctat cgtctgctcg
     1681 acaccctcga cgatgcggca ctcgcggcgc agcgacggct gaccgtaccc ttcgtgcgcc
     1741 gtgctcagcg gcccggacta gcgatgcgcg ccgcgagctc ggcgacgcgc cggccgagca
     1801 cctgcgccag cgtcttttcc tcttccgaga gcgtgggccc ggtggcgtgc gccgcgacgt
     1861 gcgaggcgcc gtagggcgag ccgcccgtgc gtgtcgtcga cagctcccgt tccgtgaacg
     1921 ggatgccgac gatgtacatg ccgtggtgca ggagcggcag catcatcgac agcagcgtcg
     1981 tttcctgtcc gccatgctgg gtctgggtcg aggtgaacac gccggccggc ttgccggcga
     2041 gcgtgccgtc gagccacagg ctcgaggtgc cgtcgaggaa atacttgagg ggcgcggcca
     2101 tgttgccgaa gcgcgtggga ctgccgagca cgagcccgcg cgtctcgcgc aggtcggcga
     2161 gcgtggcgta gggcgggccg ctcgccggca ccggtttcgc cggcgcttcg ctctcggcgc
     2221 tcaccggcgg cacggtacgc agtcgcgccc ccatgcccgc gacagcctcg atgccgcggc
     2281 agacctggcg cgcgagctcg gcggtggcac cggtgcgcga gtaatagagg acgaggatct
     2341 cgttcatggg cgcattgtag aatcgtcgcc gatggtcgcg cgcaagtgtt tggtatcggg
     2401 acgcgtgcag ggcgtcttct accgcgccac ggtggcacgc cgcgcgcgcg agctcgccat
     2461 tctgggccac gcgcgcaatc tgcccgacgg gcgcgtcgag gtgctggcct gcggtgcgcc
     2521 ggaagccgtc acgcacttca tccaggcgct atgggcgggt tccaccgcct cgagggtgac
     2581 cgccgtcgaa atcgaggaac tcgacctcgc gcccgatcag cgaccgaggg gcttcgaaac
     2641 cggctgagcg ctcacagagg ctgaaaggcc gaggtcgtcg gcgccgttcc accggctcca
     2701 gctgttgatc acgaggtcgg aataggtgcg ccggccgacg ctgccgttgt agcgcgccag
     2761 tgccttctgg atgttgttgc tctcgtaccg gaggtagtag cgcaggatcg cgcaacccat
     2821 gcggatgttc gggccgatct gcgtcagctg gtggcgctgc atgccgagct tctcgggcca
     2881 gaacggcatg atctgcatga gcccgaccgc gcctgcgtac gagacggccc agcgatcgaa
     2941 gcgactctcg atgtcgatca ccgccatcac gaggcccggg ggcaggcgca cttcgccctt
     3001 gcggtgcgcc tcgcagaaca cggtgcgcag gatctccatg cgctcggcct tgtcgggtac
     3061 gcgcttgacg aggcgcggct ccatcatcgt gaaccacacg gccgattcgt attcgtcggg
     3121 gaagcactcc gcctgggcaa tcgcgcgctc gatcaccgag cgcaggcccg cttcctcctg
     3181 ttgcccgtcg gcgggcgccg actgcgcggc gctggcgagt gcccaggccg caagcgtcgc
     3241 ggctgcaagg ccggcgcgtc gcgcccgcgg ctcagcgcgg cgcgagtcgc gagcggagga
     3301 aatccagcgc gccatcgagc gccatgtcct cgctatcggt cgcgcggcgg tgccggtact
     3361 cgagcttgcc ggcgtcgagc ccacgctcgc ccacgacgat gcgatgcggg atgccgagca
     3421 gctcggcatc cgcgaacttg gcgccggggc gcgcgtcgcg gtcgtcgagc agcacttcga
     3481 tgcctgcgcg ggtgaactct tcgtagaggc ggtcggcggt ttcacggact cgtggcgact
     3541 tcgcgaggtt gatcgggacc agtaccacct ggaacggcgc aatcggctcc ggccagatga
     3601 tgccacgatc atcgtggttc tgttcaatgg ccgccgccac gacgcgcgtc acacctatgc
     3661 catagcagcc catcagcacg gtggtgctgc gcccgccctc gtcgagcacg gcgcagttca
     3721 tcgcctcgct gtacttgcgg ccgagctgga aaatgtgccc cacctcgatg ccgcgcacga
     3781 tcgcgagcct gccggtgccc gtggggctcg ggtcgccctc gaccacgttg cggatgtcgg
     3841 cgacctgcgg ctcgggcagg tcgcggcccc agttgacgcc ggtgaggtgc gtgtcgtgcg
     3901 cgttcgcgcc acagacgaag tcggccatca ccgccgcgct gcgatcggcg atgatcgggc
     3961 acttgagccc gaccggcccg acgaaaccgg gctccgcgcc cgaggccgca agcaccgcct
     4021 cggcgctcgc catgcgcagc ggcgaggcga cccccgcgag cttctgcgcc ttcacggcat
     4081 tcagttcatg gtcgccgcgc agtacgagcg ccacgacacc gccgtcggcg ccgtcgacca
     4141 gcagcgtctt cccgcactgc gtcgccggca ccccgaggaa cccggagagc gcggcgatgg
     4201 tgcgcacttc cggcgtcgcc acgcgcgcga gtgcggccgt ggccggcggg cgcggtgcgg
     4261 ccggagccac cgcctcggcc atctcgagat tcgccgcata cgcgtcgccg tcggagaaca
     4321 cgatcgcgtc ctcgccggaa tcggcgagca cctggaactc ctccgaggcg ctgccaccga
     4381 tcgcgccgct gtcggcgcgt accgcgcgga actcgaggcc catgcgcgtg aacatgcgcg
     4441 tgtaggcctc gcgcatcgcg gcatagccct cggccatcga cgcctcatcg aggtggaagg
     4501 aatacgcatc cttcatgatg aattcgcgcc cgcgcatcac gccgaagcgc ggccggatct
     4561 cgtcgcggaa cttcgtctgg atctggtaga agttcaccgg cagctgccgg tagctctgca
     4621 gttcgcggcg cacgaagtcg gtgatcactt cctcgtgggt cgggccgatc acgaaatcgc
     4681 gctcgtgccg gtccttcagg cgcagcagct ccggaccgta ctcgccccag cgacccgact
     4741 cctgccacag ctcggcaggc tgcaccgccg gcatcagcag ctcgaacgcg ccggcccggt
     4801 ccatttcctc gcgcacgatg gtctcgacct tgcgcagggt cagcaggccg agcggagtcc
     4861 aggtgtagat gcccgcggcc aggcggcgga tcaggcccgc gcgcagcatc agccggtggc
     4921 tgacgagctc cgcctcggcg ggcgtgtctt tcatcgtatt gatcgggtag cgggaccagc
     4981 gcatcggcga aacgggctcc attggcggta cgacaggggg gcggcgcatc ttatatgatg
     5041 cgcgtcatgt ccacactgag ccccgactcc gcgccgcgca gtcgcggtgt ctacctgctg
     5101 ccgaacgcgt tcacgaccgg ctgcctgttc gccgggttct acgccatcgt ggccgccatc
     5161 gacggcaatt tcgaccgcgc cggcctcgcg gtgttcgcgg cgatgctgtt cgacggcctc
     5221 gacgggcgcg tggcgcgctg gaccggcacc gagagcgcct tcggcaagga gtacgacagt
     5281 ctggccgaca tggtggcgtt cggcctcgcc ccggcgatcg tcatgtacca gtggggcgtc
     5341 gagcgcatcg ccgagtacgg ccacacctgg ggtcgcgtcg gctgggtcgc ctgcttcttc
     5401 tatgcggtga cggcggcgct gcggcttgcg cgcttcaata cgcgcgccgc cgaggacaag
     5461 cgctacttcg aggggctgcc gagcccgtcc gccgcggcgg tggtggccgc ctacgtgtgg
     5521 ttcacgagca cgtggcgcgg cacggatgcg ctcgtcgaac tggtcgccgc cttctgcgtc
     5581 accatgcttg ccggcgcgct gatggtgtcg cgcttcccgt acatgagcct gaagcaggtc
     5641 gatgtcgaca agcgggtgcg ttactactat ttcgtcgtcg tgccgctgtt cctcgcgctg
     5701 atcgcgatcg atccgccgac gatgctgctc gcgatgttct cgacctacgc gttgtcggcg
     5761 ccggcgcaat gggtcgtgcg gcgcctgcgc cggctgtgaa cgccggcgcg caatcacgcc
     5821 gcgcgcacta tctcggggcg cttggcatcg actgctatgt gtcgcgtcat gctcccgcgg
     5881 cggcgcccgt gcccatggcc gtgcccgcaa cgccgcgcga gcctggcccc gccggcgagt
     5941 gggaagcgct gcgcgccgaa gtcgccgcct gcacgaaatg cagcctctgc gagacgcgca
     6001 cccagacggt cttcggggtc ggcgaccgcc gcgcgcgctg tctcatcgtc ggtgaggcgc
     6061 ccggcgccga ggaggaccgc cgcggcgagc ccttcgtcgg gcgcgccggc cagctcctcg
     6121 actcgatgct gcgtgccgtg cgcctctccc gcgacacggt gttcatcgcc aatgtgctga
     6181 agtgccgccc gccgggcaac cgcgatccga gacccgacga ggcagcgagt tgcctgccgt
     6241 acctcgagcg gcagatcgcg ctgatcgaac cggccgtcat ggtagcggtc gggcgcgtcc
     6301 cggcgcaggt gctgctcggc agcgatgcgg cggtcgcccg gctgcgcgga cgcgtgcacc
     6361 agttcgggga gcgggcgatc ccgctcgtcg tgacctacca tccggcctac ctgctgcgcg
     6421 cgccggccga gaagcgcaag gcctgggacg acctgaagct cgcgagggac ctgctcgatg
     6481 gccgcggagc gcgatgacct gccggtaccg gaggtgcagc tgcggccgat gacggaggcc
     6541 gacgtcgacc gcgtcgccgc gctcgagcgc gagtcctacc cgttcccctg gagcgagggc
     6601 atcttccgcg actgcctgcg cgtgggctac gtctgccgtg tcgtggaact gggcccggcc
     6661 ctgatcggct atggcgttat gagccacggg gccggcgagg cgcatatcct caatctgtgc
     6721 gtgcgcgagg cgatgcgcgg gcgcggcatc gggcggagcc tcctgcgcca cctgctccag
     6781 caggccgccg gcgccggcat ggacgaggcc ttcctcgagg tgcgcccgtc gaatctcgcc
     6841 gcgatccgcc tgtaccagtc gatgggcttc gagccggtcg gcgtgcgccg cagctattac
     6901 caggcggtag gcgggcgcga ggatgcgacc gtgctgcgcc tcgacctgcg cggcctgcgt
     6961 tagcgcagcg cgagcggcag ctgggtcgtt tccttcacca ctgtcatcgc gatcgaggaa
     7021 ctgagcgact ggatgcccgg gatcttcgag agctggtcga ggaagaaggc ttcgtacgcc
     7081 ttgatgtcgc gcgcgacgat gcgcagcagg aagtcccagt cgcccatcag cgtgtagcac
     7141 tcgagcactt cggggtggcg gcggatggcc tggtcgaagc gcgcgaggtt gtcgcgcccg
     7201 tgggccacga gcttcacatg ggcgaacacc gtgacaccga accccacctt ttcgcgatcg
     7261 aggatcgcga cgcgctggcg gatcacgccc tgttcctcga ggcggcgtac ccggcgccag
     7321 caggtggtga ccgcgaggtt cacccgttcg gcgagttccg cggcggacag cgagccgtcc
     7381 tgctgcagga catcgaggat gcgcaggtcg gtgcggtcga ggccagactg gggcatatgt
     7441 tccgctactg gcaaataagt ggaataatta tttcggattc tgggtaattc cggtcatctt
     7501 tgcaatgctc atggcaggcc atcggcgcat agtgggcggt ggggtcaagg agtcgtgata
     7561 tggcgagtcg tccgcgcacg agattgcatg tgcctcagcc gccggcccgg ccgggcgaca
     7621 aggcggattt cagctacctg aaactgcagc ccgcgggcaa gcagccgcgc ccggacgtga
     7681 aagtgtcggc ccaggctacg ctgccgctgg caaccggcat cgtgcgggtg ctcgatgacg
     7741 agcatcgtgc ggtcggcgcg tgggatccgc atctcgagcc cgaggtgctg cagatgggcc
     7801 tgcgccacat gctgctcacg cgcatcttcg acgagcgcat gcagcgcacc cagcgacagg
     7861 gccgcatttc cttctacatg cgcagccacg gcgaggaggc cgtctcggtc gcacagacca
     7921 tggcgctgcg cccgggcgac atgctgtttc cctcctaccg caaccagggc atctacatgg
     7981 tgcgtggccg gccgctcgtc gacctgatgt gccagctgct gtcgaacacg cgcgacatgt
     8041 gcaagggccg ccagctgccg gtgatgtacc actggcgcga gggcaacgtc ttctcgatct
     8101 cgggcaatct cgccacgcag ttcccgcagg ccgtcggctg ggcgatggcc gcggcgatca
     8161 agggcgagga ccatgtcgcg gcgacctgga taggcgaagg ctcgagcgcg gaaagcgact
     8221 tccacgccgg catggtgttc gccgaggtct accaggcgcc cgtactgctc aacatcgtca
     8281 acaaccagtg ggcgatctcg accttccagg ggtttgcggg cggggagaag tccactttcg
     8341 cggcacgcgg ccccggctac ggggtcgccg gcatccgcgt cgacggcaac gatttcctgg
     8401 ccgtgcatgc ggtgactcag tgggccgccg agcgcgcgcg caggggcggc ggtcccacgc
     8461 tgatcgaaca tgtgacctat cgtgcctcgg cccattcgac cagcgacgat ccgtcgcgct
     8521 accgtccgaa ggacgaatat gagcgctggc cgctcggcga tccggtcgag cgcctcaagc
     8581 agcatctcat cgcgctgggt gagtggtcgg aggagcgcca cacggcgctc gcgcaggaac
     8641 tcgacgagca ggtatcggcc gcatggcgcg aggcgacccg gtacggcacc ctgaacgagg
     8701 gcccgcggct cgatgccaac ctcatgttcg aggatgtatt caaggagatg cccgagcatc
     8761 tcgtgcgcca gcgtgaacaa ctgcgcgcgg agcggggctg acatggcgcg catgaacatg
     8821 atccaggcgt tgaactccgc gatggacgtc atgctcggtc gcgacccggg tgtcgtggtg
     8881 ctcggtgagg acgtcggcta tttcggcggc gtgttccgca ccaccgacgg cctgcagcgc
     8941 aaatacggcg agcatcgcgt gctcgatacg ccgatttccg agctcggcat cgtcggcacg
     9001 tcgatcggca tggccgtcaa cggcctgcgc ccggtcgccg agatccagtt cgccgattac
     9061 atctatcccg gctacgacca gatcgtgagc gagctcgcgc ggctgcgcta ccgcagcgcc
     9121 ggcgagttct tcgcgccggt cacgatccgc acgccgtgcg gcggcggcat ccgcggcggc
     9181 cagacccact cgcagagtcc cgaggccgtg ttcgcgcaga tctgcggcat caaggt
//
LOCUS       DAOQVY010000096         9125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_8471_length_9125_cov_5.741014, whole genome shotgun sequence.
ACCESSION   DAOQVY010000096 DAOQVY010000000
VERSION     DAOQVY010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9125
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8471_length_9125_cov_5.741014"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..554
                     /gene="gcvH"
                     /locus_tag="PKN91_09300"
     CDS             159..554
                     /gene="gcvH"
                     /locus_tag="PKN91_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNS28309.1"
                     /translation="MTQIPADIRYAKSHEWAKLLGDGSVEVGISDHAQGALGDLVFVE
                     VPAVGRTLDAGEACAVVESVKAASDVYSPVAGTVTEVNGELANAPELINSDPYGAGWL
                     FRLRPAAAGALDALLTAADYEAVLQAENA"
     gene            576..1949
                     /gene="gcvPA"
                     /locus_tag="PKN91_09305"
     CDS             576..1949
                     /gene="gcvPA"
                     /locus_tag="PKN91_09305"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNS28310.1"
                     /translation="MPFIPHTEEDIREMLAAIGAPSIEALFDEIPQELRIKSLTGIPE
                     GLAELEVGRLMTERAKLDGTPLSFIGAGAYEHHIPSAVWAITSRGEIYSAYTPYQAEA
                     SQGTLQAIYEYQTMIASLTGMQVSNATLYDGGSALAEAALMAVRAHRKSTSMRLLVPA
                     SVHPHYRKVAVAMAGNQDLVFDTIPYVRETGRIDMAALTAHAGEDITAVVIPQPNFFG
                     VLEDVDALTDWAHAQGALVIAVVNPTSLALLKPPGEWGAKGVDIVVGEGQPLGVPLSS
                     GGPYFGFMTTRMEHVRQMPGRIVGRTVDLDGREGFTLTLQAREQHIRRGKATSNICTN
                     QGLLMTAATIYMSIMGPEGLERVAAQSAQRTRELVAALTRVKGVRKAFDSPHFHEAVL
                     LLERPAAVVLRELADRGVLGGFDLAADYPELGSALLVCATETKTAADIDRYSAALAEA
                     LRAARAA"
     gene            1972..3450
                     /gene="gcvPB"
                     /locus_tag="PKN91_09310"
     CDS             1972..3450
                     /gene="gcvPB"
                     /locus_tag="PKN91_09310"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNS28311.1"
                     /translation="MPNRREKLIFEYSRPGRGAQGQWPAKAAVPADIPATLRRASRPL
                     LPEVSELEAVRHFTRLSQLNFSIDTHFYPLGSCTMKYNPKACHQYAMLPEFLGRHPLG
                     PESHGQGFLACMYELQEMLKAVTGMRGVSLAPMAGAHGEFAGVAMIRAYHRARGDHAR
                     NEIIVPEAAHGTNPATATMCGCVVREIPVMADGDVDFEALKTAVGPQTAGIMLTNPST
                     LGVFERRIKEVAGIVHAAGGLLYYDGANLNAILGKVRPGDMGFDVIHMNLHKTFSTPH
                     GGGGPGSGAVGVSERLLPFMPIPVVGRDGEQYRWLGERDLPQSIGRLSAFMGNAGVLL
                     RAYVYMRMLGREGMARVGDFATLNANYLLAKLTAAGFDAAYPSRRASHEFIITAKRQA
                     REHNVTAMDIAKRLLDFGFHAPTTYFPLLVPECLLIEPSETESKEALDAFIDAMVAIK
                     REAEGDTQLLKGAPHTLPVRRLDDVRAARQLDLAWRPAATRG"
     gene            3447..3812
                     /locus_tag="PKN91_09315"
     CDS             3447..3812
                     /locus_tag="PKN91_09315"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008223741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HNS28312.1"
                     /translation="MKREKPQGFTRLFRAFGASGKGLAGAWREEAAFRQELAFAACAL
                     PLGLWLGRSGVERALLVAPVLLILVVELVNSAIEATVDRIGPERHLLAGLAKDLGSAA
                     VLTSFVLCGTVWALVLLGR"
     gene            3835..4767
                     /locus_tag="PKN91_09320"
     CDS             3835..4767
                     /locus_tag="PKN91_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNS28313.1"
                     /translation="MSALICGSVAFDTVMVFDGRFKDHILAERIHLLNVAFLVPALRR
                     NFGGCAGNIAFNLKLLGGEGHAMATVGSDFGPYEAWMRANGLPLTYIDKLDTEYTAQA
                     YITTDLDSNQITAFHPGAMMFAHRNRVPTDAGVSIGVVAPENRDGMIQHSAQFAAAGI
                     PVLFDPGQGLPMFNGEDLRRFISEATWIAVNDYEGSLLAERAGQSLEEIASQVKALIV
                     TRGGEGSLIHADGKVHEIPAARVGALADPTGCGDAYRAGLLYGLQNGLDWPTTGRIAS
                     LMGAIKIEHHGTQNHRFTRADFAARFRESFGREL"
     gene            4769..7663
                     /locus_tag="PKN91_09325"
     CDS             4769..7663
                     /locus_tag="PKN91_09325"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNS28314.1"
                     /translation="MTRRFWISLLAGLIATTAQAQLAPQQSQPQPQPGQAGDPSQGLA
                     PPQPVVGVEPADSGWTRTLERIAQSVVAIEIDQTRAFDTEWNASSQATGFVVDAERGL
                     ILTNRHVVTPGPVIAEATFLNREEVQLYPVYRDPVHDFGFYRYDPSKLRFITPKSLPL
                     YPAGAQVGREIRVVGNNAGEQLSILAGTLAKLDREAPKYGVGKYNDFNTFYVQAASGT
                     SGGSSGSPVIDIQGRVVALNAGGSSAAASSFYLPLARVKRALELIQAGKPVSRGTLQT
                     TFEYTPFDELRRLGLQAETEAAVRKSFPEFTGMLVVAEVQPGSAAADVLYPGDVLVRV
                     NGKYLTQFEPLGAVLDDSVGAPVELELERGGQLVSTTITVGDLHVITPDEYLEFGDAV
                     VNTLSYQMARHFNIPVRGAYVANPGYALGAAGIPRGAVIKAVNGAAIDSIRDLEAAFD
                     KLGDGDRTSVRYSTIDDPNGSQLRSVRMDRRWFPAQRCKRNDATGFWDCSTLAAGPAP
                     RAPVVGSTSFLLPGDTRIARIVPSLVTVGFDMPYAVAGVTERNYHGTGLVVDAERGLV
                     VVDRNTVPVSLGDVRITFAGTLEIPAKVVFVHPLHNLAIVQYDPTLVGETPVRSAKLG
                     TKPLRAGDDVWVVGLSPDTGLKSRATSIADIDPIVLPLSRTMQFREANLEVMQLVNGP
                     QDFDGVLLDRSGAVAGVWSSFASESGREVVQANRGVPIDIVADMVARARDGRPIYSLE
                     VELLPQTLAVARRLGLSDDWVRQIEGAGGRTRQVLTITRVVGGSPAAQILQQGDLLLA
                     IDGRVMRDFRDVERAVADKPTVLVTVWRGSEAREFDVATAELTGVDLTRMVQWAGATL
                     HAPHRAMLVQRGIAPEGVYVAYFAYGSPATRAGLFPGRRIVEVDGQPTPDLDSFLRLV
                     TGRPDRASVRLKTISWNNAPEVITLKLDKHYWPAYELRRIGARWERYSLE"
     gene            7728..8759
                     /locus_tag="PKN91_09330"
     CDS             7728..8759
                     /locus_tag="PKN91_09330"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNS28315.1"
                     /translation="MVRIVRATQAEIDEAVTALRDGELVAFPTETVYGLGANAQNPAA
                     VQKIFEVKGRPTTHPVIVHLDSPKYLHRWVREVPPAAQALAERFWPGPLTLVMPRAAN
                     VHDIVTGGQDTIAIRVPAHPMAQQLLTAFGGGIAAPSANRYGRLSPTRAEHVREEFGD
                     AVRVVLDGGESQVGLESTIVACDETGVRLLRPGAVTLAQLRQVVGDVQVGPPGAAPRV
                     PGSTPTHYAPATPMTIVPADEIDTRAEDASEGGRRIAVLAQRLPLKTYPSVTWINAGR
                     RPDAYAHDLYANLRTLDTAGCEQILVQEVPTDDERWDAIRDRLLRGAAASEVDGADLA
                     ATDAPGVMP"
     gene            8805..>9125
                     /locus_tag="PKN91_09335"
     CDS             8805..>9125
                     /locus_tag="PKN91_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28316.1"
                     /translation="MAIVAAGIPWKQLILLAPDIAKGAKAIWGQWKSKPVPEPIDANK
                     SPGDQLAAISKRLLALEANEANQSRLVSEMADQVQVIAVGLRETAARQTVIIWVAGAA
                     LLLAI"
BASE COUNT         1464 a         3123 c         3128 g         1410 t
ORIGIN      
        1 gccgtcgctg cagaagtcga ttgcgctcgc gcgcgtgccg gccgcaaccg gcgaccgcgt
       61 gcaggtcgac atccgtggca gactcctcga tgcgcgtgtc gtgcgtccgc cgttcgtgcg
      121 caacggcaaa tcgctgattt cctgaccgga gagtttccat gacccagatt cccgcagaca
      181 tcaggtacgc gaagagccac gagtgggcga aactgctcgg cgatggcagc gtggaggtcg
      241 gcatcagcga tcatgcacag ggcgcgctcg gcgacctcgt gttcgtcgaa gtgccggccg
      301 tcgggcgcac gcttgacgcc ggcgaggctt gcgcggtcgt cgagtcggtg aaagccgcgt
      361 ccgacgtcta cagcccggtg gcgggtaccg tcaccgaggt caacggcgag ctggccaacg
      421 cccccgaact gatcaactcg gatccctacg gcgccggatg gctctttcgc ctgcgcccgg
      481 ctgcggcggg cgccctcgat gcgctgctga cggccgccga ctacgaagcg gtactgcagg
      541 ccgagaacgc ctgacgcgcg ggcaaggagc gaggcatgcc gttcattccg cacaccgagg
      601 aagacatccg cgagatgctc gccgccatcg gcgcgccctc gatcgaagcg ctgttcgacg
      661 agatcccaca ggaacttcgc atcaagtcgc tgaccggcat tcccgagggc ctcgcggaac
      721 tcgaggtcgg ccggttgatg accgagcggg cgaaactcga cggcacgccg ctgtcgttca
      781 tcggcgcggg cgcctacgag caccacatac cctcggcggt gtgggcgatc acctcgcgcg
      841 gcgaaatcta cagcgcctat acgccctacc aggccgaagc gagccagggc acgctgcagg
      901 cgatctatga ataccagacg atgatcgcct cgctcacggg catgcaggtg tcgaacgcga
      961 cgctgtacga cggcggctcg gcgctcgcgg aagcggcgct gatggccgtg cgcgcgcacc
     1021 gcaagtcgac atcgatgcgg ctgctcgtgc ccgccagcgt gcatccgcat taccgcaagg
     1081 ttgccgtggc gatggccggc aaccaggacc tcgtgttcga cacgatcccg tacgtgcgcg
     1141 agaccggccg catcgacatg gccgcgctga cggcgcatgc gggcgaggac atcaccgcgg
     1201 tcgtgatacc gcagccgaat ttcttcggcg tgctggaaga cgtcgatgcg ctcaccgact
     1261 gggcgcacgc gcagggcgcg ctcgtcatcg ccgtcgtcaa tccgacctcg ctggcgctgc
     1321 tcaagccgcc gggcgagtgg ggcgcgaagg gcgtcgacat cgtcgtcggc gagggccagc
     1381 cgctcggcgt accgctgtcg tccggcgggc cgtacttcgg cttcatgacc acgcgcatgg
     1441 agcacgtgcg ccagatgccg ggccgcatcg ttggccgcac cgtcgacctc gacggccgcg
     1501 agggcttcac gctcacgttg caggcgcgcg agcagcacat ccggcgcggc aaggcgacct
     1561 cgaacatctg caccaaccag ggcctgctga tgaccgcggc caccatctac atgtcgatca
     1621 tgggtccgga aggcctcgag cgtgtggccg cgcagtcggc gcagcgcacg cgcgagctgg
     1681 ttgcggcgct gactcgcgtc aagggggtgc gcaaggcatt cgattccccg cacttccacg
     1741 aggcggtgct gctgctcgag cgtccggcgg cggtcgtgct gcgtgaactg gcggaccgcg
     1801 gcgtgctcgg cggcttcgat ctcgccgccg actatccgga actcggctcc gcgctgctcg
     1861 tgtgcgcgac ggagacgaag accgccgccg atatcgaccg gtattccgcc gcgctggccg
     1921 aagccctgcg cgccgcgcgc gccgcctgaa ccgggacaca ggacctaccc gatgccgaat
     1981 cgccgcgaga agctgatttt cgagtactcc cgcccaggcc gcggagcgca gggccagtgg
     2041 ccggcgaaag ccgcggtacc ggcggatatc ccggcgacgc tgcgccgtgc cagccggccg
     2101 ctcctgccgg aagtgagcga gctcgaggcc gtgcgtcact tcacgcgcct gtcgcagctg
     2161 aacttctcga tcgacacgca tttctatccg ctcggctcgt gcacgatgaa gtacaacccg
     2221 aaggcgtgcc accagtacgc gatgctgccc gagttcctcg ggcgccatcc gctcggtccc
     2281 gagtcgcacg gccagggctt cctcgcctgc atgtacgagc tgcaggaaat gctgaaggcc
     2341 gtgaccggca tgcgcggcgt atcgctcgcg ccgatggcgg gcgcacacgg cgaattcgcc
     2401 ggcgtggcga tgatccgcgc ctaccaccgc gcgcgcggcg atcatgcgcg caacgagatc
     2461 atcgtgcccg aggcggcgca cggcacgaac cccgcgaccg cgaccatgtg cggctgcgtg
     2521 gtgcgggaaa tcccggtgat ggccgatggc gacgtcgatt tcgaggcgct caagaccgcg
     2581 gtcgggccgc agacggccgg catcatgctc accaacccct cgacactcgg cgtgttcgaa
     2641 cggcgcatca aggaagtcgc cggcatcgtg catgccgcgg gcgggctcct ctattacgac
     2701 ggcgcgaacc tcaacgcgat cctcggcaag gtgcgccccg gcgacatggg gttcgacgtg
     2761 atccacatga acctgcacaa gaccttctcg acgccgcacg gcggcggcgg gccgggctcg
     2821 ggggccgtgg gcgtcagcga gcgcctgctg cctttcatgc ctatcccggt cgtcgggcgc
     2881 gacggcgagc agtatcgctg gctcggcgag cgcgacctgc cgcagtcgat cggccgcctg
     2941 tccgcgttca tgggcaatgc cggcgtgctg ctgcgggcct atgtgtacat gcgcatgctc
     3001 ggccgcgaag gcatggcgcg cgtcggcgac ttcgccacgt tgaatgcgaa ctatctgctc
     3061 gcgaaactga ctgcggcggg cttcgatgcc gcctacccga gccggcgcgc gagccacgag
     3121 ttcatcatca ccgcgaagcg ccaggcgcgc gagcacaatg tcaccgcgat ggacatcgcc
     3181 aagcggctgc tcgatttcgg cttccacgcg ccgaccacct atttcccgct gctggtgccc
     3241 gagtgcctgc tgatcgagcc gagcgagacc gagagcaagg aggcgctcga tgccttcatc
     3301 gatgcgatgg tggcgatcaa gcgcgaggcg gagggcgaca cgcagttgct gaagggcgcg
     3361 ccgcacacgc tgccggtgcg ccggctcgac gacgtgcgcg cggcgcgcca gctcgacctc
     3421 gcctggcgac ccgcagcgac ccggggatga agcgcgagaa accgcaaggc ttcacgcggc
     3481 ttttccgggc cttcggcgcg agcggcaagg gcctggcggg cgcgtggcgc gaggaggcgg
     3541 ccttccgcca ggagctggcc ttcgcggctt gtgcgctgcc gctcggcctg tggcttggac
     3601 gcagcggcgt cgaacgtgct ctactcgtcg caccggtgct gctcatactc gtcgtcgaac
     3661 tcgtgaacag tgcgatcgag gcgaccgtgg atcgcatcgg ccccgaacgc cacttgctgg
     3721 ccggcctcgc caaggacctg ggctccgcgg cggtcctgac gtcgttcgtg ctttgcggta
     3781 ccgtgtgggc gctggtgctc ctcggccgct gattccaccc ccactcggag cagtatgtca
     3841 gcactaatct gcggctcggt cgccttcgat acggtcatgg ttttcgacgg ccgcttcaag
     3901 gaccacatcc tggcggagcg catccacctg ctcaatgtcg ccttcctggt gccggcgctg
     3961 cgccgcaact tcggtggttg cgcgggcaac atcgccttca acctcaagct gctcggcggc
     4021 gagggccacg cgatggccac cgtcggcagc gacttcgggc cctacgaggc ctggatgcgc
     4081 gccaacggcc tgccgctcac ctacatcgac aagctcgata ccgagtacac ggcacaggcg
     4141 tacatcacga ccgatctcga ctcgaaccag atcacggcct tccatcccgg cgcgatgatg
     4201 ttcgcgcacc gcaaccgcgt gcccaccgat gcgggcgtca gcatcggcgt ggtcgcgccg
     4261 gagaaccgcg acggcatgat ccagcactcc gcgcagttcg cagcggccgg catccccgtg
     4321 ctgttcgatc ccggccaggg cctgccgatg ttcaacggcg aggatctgcg caggttcatc
     4381 agcgaggcga cgtggatcgc cgtcaacgac tacgagggga gcctcctcgc cgagcgcgcc
     4441 ggacagtcac tcgaggagat cgcctcgcag gtcaaggcgc tgatcgtgac ccgcggcggc
     4501 gaaggctcgc tcatccatgc ggacggcaag gtgcacgaaa tccccgccgc gcgcgtcggt
     4561 gcgctcgccg acccgacggg ttgcggtgac gcttatcggg ccggattgct gtacgggctg
     4621 cagaacggcc tcgactggcc gaccaccggt cgcattgctt cgctgatggg ggcgatcaag
     4681 atcgagcacc acggcacgca gaaccaccgg ttcacgcgag cggatttcgc cgcccgtttc
     4741 agggagagct tcggccggga gctttagcat gacgagaaga ttctggatca gcctgctcgc
     4801 gggactcatc gccacgacgg cccaggcgca gctcgcgccc cagcagtcgc agccgcagcc
     4861 gcagcccgga caggccggcg atccttcgca gggcctcgcg ccgccccaac cggtggttgg
     4921 cgtcgagccg gccgacagcg gctggacgcg cacgctcgag cgtatcgcgc agagcgtcgt
     4981 cgcgatcgag atcgaccaga cgcgcgcgtt cgacaccgag tggaatgcgt cctcgcaggc
     5041 gacgggcttc gtcgtcgatg ccgaacgcgg cctgatcctc accaaccggc atgtcgtgac
     5101 gccgggcccg gtgattgccg aggccacctt cctgaatcgc gaggaagtgc agctgtatcc
     5161 ggtgtaccgc gatcccgtgc acgacttcgg cttctatcgc tacgatccgt cgaagctgcg
     5221 cttcatcacg ccgaagtccc tgccgctgta tcccgcgggc gcgcaggtcg ggcgcgagat
     5281 tcgcgtggtc ggcaacaacg cgggcgagca attgtcgatc cttgccggga ccctcgcgaa
     5341 gctcgaccgc gaggcgccga agtacggcgt cggcaagtac aacgatttca acactttcta
     5401 cgtgcaggcc gcgtcgggga cttcgggtgg ttcatcgggc tcgcccgtga tcgacatcca
     5461 ggggcgcgtg gtcgcgctca atgccggtgg atcgagcgcg gcggcgtcga gtttctacct
     5521 gccgctcgcg cgcgtgaagc gcgcgctcga gctgatccag gcgggcaagc cggtctcgcg
     5581 cggcacgctg cagaccacct tcgagtacac cccgttcgat gaactgcgcc ggctcggcct
     5641 gcaggctgag accgaagcgg cagtgcggaa gtcgtttccg gaattcaccg gcatgctggt
     5701 ggtcgccgaa gtgcagccgg gcagcgccgc ggcggacgtg ctgtatccgg gcgacgtgct
     5761 ggtacgggtc aacggcaagt acctcacgca gttcgagccg ttgggcgccg tgctcgatga
     5821 cagcgtgggc gcgccggtcg aactcgagct cgagcggggc gggcagctgg tgtcgacgac
     5881 catcacggtg ggcgacctgc atgtgatcac gcccgatgag tacctcgagt tcggcgacgc
     5941 ggtcgtcaac acgctctcct accagatggc acgccacttc aatattccgg tgcgcggtgc
     6001 ctacgtcgcc aatccgggct acgcactcgg cgcggcgggc attccgcgcg gcgcggtgat
     6061 caaggccgtg aacggcgcgg cgatcgacag catcagggat cttgaggccg cattcgacaa
     6121 gctgggcgac ggcgaccgca catcggtgcg ctactccacc atcgacgacc cgaacggcag
     6181 ccagctgcgc tccgtgcgca tggatcgacg ctggttcccg gcgcagcgct gcaagcgcaa
     6241 cgacgcgacc gggttctggg actgcagcac gctcgccgcg ggccccgcgc ccagggcgcc
     6301 ggtggtcggc agcaccagct tcctgctgcc tggcgatacg cgcatcgcac gcatcgtgcc
     6361 ctcgctcgtc acggtgggct tcgacatgcc gtatgccgtg gcgggcgtga ccgagcgcaa
     6421 ctaccacggc accggtctgg tcgtcgatgc cgaacgcggc ctcgtggtcg tcgaccgcaa
     6481 cacggtgccg gtgtcgctcg gcgatgtgcg catcactttt gccggcacgc tcgagatccc
     6541 ggcgaaggtc gtgttcgtgc acccgctgca caatctcgcg atcgtgcagt acgacccgac
     6601 gctggtcggt gagactccgg tgcgcagcgc gaagcttggc acgaagccgc tgcgcgccgg
     6661 cgatgatgtc tgggtcgtcg gcctgtcgcc ggacaccggc ctcaagtcgc gggccacgag
     6721 cattgccgac atcgacccga tcgtgctgcc gctctcgcgc acgatgcagt ttcgcgaggc
     6781 gaacctcgag gtgatgcagc tcgtcaacgg accgcaggat ttcgacggcg tgctgctcga
     6841 caggtcaggc gcggtggcgg gcgtgtggtc gagcttcgcc tccgagagcg ggcgcgaagt
     6901 ggtgcaggcc aatcgcggcg tgccgatcga tatcgtcgcg gacatggtcg cgcgcgcgcg
     6961 tgacgggcgg ccgatctact cgctcgaagt ggagttgctg ccgcagaccc tggcggtggc
     7021 acgccggctc gggctctcgg acgattgggt aaggcagatc gagggcgcgg gcggccgcac
     7081 gcgccaggtg ctgacgatca cgcgcgtggt cggtggctcg ccggcggcgc agatcctgca
     7141 gcagggcgac ctgctgctcg cgatcgacgg cagagtcatg cgcgacttca gggatgtcga
     7201 acgcgccgtg gcggacaagc ctacggtact ggtcacggta tggcgcggca gcgaggcacg
     7261 cgagttcgac gtggcgaccg cggaactcac cggtgtcgac ctgacccgca tggtccagtg
     7321 ggcgggcgcg acgctgcatg cgccgcaccg cgcgatgctg gtgcagcgtg gcatcgcccc
     7381 ggagggcgtc tatgtggcgt acttcgccta cggctcgcca gccacccgcg cggggctctt
     7441 cccggggcgg cgcatcgtcg aggtcgacgg ccagccgacc cccgatctcg acagctttct
     7501 caggctcgtc accggccggc ccgaccgggc ctcggtgcgg ctgaagacga tttcgtggaa
     7561 caacgcgccc gaggtcatca ccctgaagct cgacaagcac tactggccgg cctacgaatt
     7621 gcgccggatc ggggcgcgct gggagcggta ttcgctcgaa tagcggtctt tccacaaagt
     7681 gaaccgggtc ggccttgcat cggccggcgg cttgctagaa tcaataaatg gttcgtatcg
     7741 tccgcgcaac acaggccgaa atcgacgagg ccgtcacggc gctccgcgac ggcgaactcg
     7801 tcgcctttcc aaccgagacg gtctacggac tcggcgcgaa tgcgcagaat cccgccgccg
     7861 tgcagaagat cttcgaggtg aagggccggc caacgaccca tcccgtcatc gtgcacctcg
     7921 acagccccaa gtacctgcac cgctgggtgc gggaagtgcc accggcagca caggcgctcg
     7981 ccgagcgctt ctggcccgga ccgctgactc tggtgatgcc gcgcgccgcg aacgtgcacg
     8041 acatcgtcac gggcggacag gacacgatcg ccatccgcgt gcccgcgcat ccgatggccc
     8101 agcagctgct caccgcgttc ggcggcggca tcgcggcacc ctcggcaaac cgctatgggc
     8161 gcctgtcgcc gacccgcgcc gagcatgtgc gtgaagagtt cggcgacgcc gtgcgcgtgg
     8221 tgctcgacgg cggcgaatcg caggtcggcc tcgagtcgac gatcgtcgcc tgcgacgaga
     8281 ccggcgtgcg cctgttgcga cccggtgcgg tgactctcgc gcaattgcgc caggtggtcg
     8341 gcgacgtgca ggtcggcccg cccggtgccg cgccgcgcgt gccgggcagc acgccgacgc
     8401 attacgcgcc ggcgacaccc atgaccatcg tgcccgccga cgaaatcgac acgcgtgccg
     8461 aagacgcctc ggagggcggg cggcgcatcg ccgtactcgc gcagcgcctg ccgctcaaga
     8521 cctatccctc cgtcacctgg atcaacgcgg gccggcgccc cgacgcctac gcgcacgacc
     8581 tgtacgccaa cctgcgtacg ctcgacacgg ccgggtgcga gcagatcctc gtgcaggagg
     8641 tgccaaccga tgacgagaga tgggatgcga tccgtgatcg cctgctgcgc ggtgcggcgg
     8701 cgagcgaagt ggacggtgcc gacctcgcgg cgacggatgc gccgggagtc atgccctgac
     8761 gggcgcgggc gcgggcgcga tagcggtctg aggagattct gcatatggca atagtcgcgg
     8821 cgggcattcc ctggaagcag ctcatcctgc tggcgcccga tatcgccaag ggtgcgaagg
     8881 ccatctgggg acaatggaaa tcgaagcccg tgcccgagcc gatcgacgcc aacaaatcgc
     8941 cgggcgacca gctcgccgcc atctcgaagc ggctgctggc gctcgaggcc aacgaggcga
     9001 atcaatccag gctggtcagc gagatggccg accaggtgca ggtcattgcg gtaggcctgc
     9061 gggaaaccgc cgctcgccag accgtgatca tctgggtcgc cggcgcagcg ctgctgctcg
     9121 ccatc
//
LOCUS       DAOQVY010000097         9099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_8495_length_9099_cov_4.884454, whole genome shotgun sequence.
ACCESSION   DAOQVY010000097 DAOQVY010000000
VERSION     DAOQVY010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9099
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8495_length_9099_cov_4.884454"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PKN91_09340"
     CDS             <1..933
                     /locus_tag="PKN91_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNS28317.1"
                     /translation="GANPVSQLAFTLSNGFTIVEYYLARGMRIDDFAPNLSFFFSNGM
                     DPEYVVIGRVARRIWARAMRERYGAGPRSQMLKYHVQTSGRSLHAQEIQFNDIRTTLQ
                     AMYALFDNCNSLHTNAFDEALTTPTEDSVRRAVAIQLIINRELGLNRNQNPWQGSFII
                     DYLTDMVEEAVYREFESISARGGVLGAMETMYQRGKIQEESLHYETKKHDGSLPLIGV
                     NTFLSGHDDSEARANVALIRSTEEEKQDQVAAVRAFQQRHAAEAPVALARLQQVAAGG
                     GNVFAELMETVKVCSLGQISRALYEVGGQYRRNV"
     gene            complement(930..2177)
                     /locus_tag="PKN91_09345"
     CDS             complement(930..2177)
                     /locus_tag="PKN91_09345"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF001933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HNS28318.1"
                     /translation="MRVVILGGGVIGVTAAWYLARAGAAVEVIERQPGVALETSFANA
                     GEISPGYASPWAGPGMPLKAIKWLFERHAPLVVRPGLDPSMYLWILRMLRNCTAARYD
                     LNKSRMVPIAEYSRDLLRELRTQTGIRYDERTRGTLQLFRTRPQFDGAASDIAVLARL
                     GVPYELLDAAGCIGAEPGLAGVRHLIVGGLRLPDDETGDCHLFTQRLAALAQAQGVTF
                     RCGVSILGFERAGDRIESVLTDAGPVKADAYVLALGSYSTPMARQLGFRLPVYPVKGY
                     SLTAPIVDERKAPQSTVMDESYKIAITRLGDRIRVGGTAELSGYNNRLHPRRRATLEL
                     SVGSLFPGGADYPQAEFWSGLRPMTPDGTPFVSASPLRNLWLNTGHGTLGWTMACGSG
                     RVIADLVTGRTPDIDVRGLALGR"
     gene            complement(2212..2958)
                     /locus_tag="PKN91_09350"
     CDS             complement(2212..2958)
                     /locus_tag="PKN91_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNS28319.1"
                     /translation="MSRAVRLPPPLNWLYTLYALSVFVPLGLLALLGVLLLPTMRLRR
                     STAHYIARAWLWLTGMRLDILRPDHLPAGQCVVVANHASYLDGFVMTAALPPRFGFVI
                     KREASRLPLAGLLLARIGSEFVERFNRHKGASDARRVVRNAGNGHSFVFFPEGTFAHQ
                     PGLMKFHSGAFMTAARAGCPVVPCVIRGTRDAWPAKRLGLLPARLEVQFLPPLPAAQN
                     GDEQASVTLRDCARRDILAALGEPDLSEAA"
     gene            3082..4014
                     /gene="xerD"
                     /locus_tag="PKN91_09355"
     CDS             3082..4014
                     /gene="xerD"
                     /locus_tag="PKN91_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNS28320.1"
                     /translation="MARPATANELRKPAGETDPAVTRFIDAVWMERGLSSNTLAAYRA
                     DLTALSRWLAEHGREITQTTRSDLMDFIAFRVKAGARPRSTARQLSSFRRFFRHLVRE
                     GTLREDPTAQIAMPKIGRSLPRSLSEGEVESLLNAPAVADPLGNRDRAMLEVLYATGL
                     RVSELVSLRHSQVNLNQGVLRIVGKGNRERLIPLGEEAVRWLSEFVRGPRNEILLDRT
                     TDYLFPTRRGDRMTRQAFWHIIKRYARKAGVERDLSPHTLRHAFATHLLNHGADLRVV
                     QMLLGHSDLSTTQIYTHVARERMKDLHAQHHPRG"
     gene            4093..4803
                     /locus_tag="PKN91_09360"
     CDS             4093..4803
                     /locus_tag="PKN91_09360"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HNS28321.1"
                     /translation="MLRAKTFLLLLALGLVPFAGHADPRVELAKKFPGATAEDLTPSR
                     IPGVYELRRGADLVYVTPDGRYALMGDLYDLRDNSNVTENRRRALRVALLEKVPDRQA
                     LIFSPKDPKYSIDVFTDIDCGYCRKLHSQIVEYNKLGVRVRYLFYPRSGPNTESWKKA
                     ENVWCAANRNDALTRAKRGETVKSPACGATPVARQYELGEDLGISGTPAIVLANGELL
                     PGYVPPAVLVDHLKTAAR"
     gene            complement(4804..5127)
                     /locus_tag="PKN91_09365"
     CDS             complement(4804..5127)
                     /locus_tag="PKN91_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016138692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNS28322.1"
                     /translation="MAFVVTENCIKCKYMDCVEVCPVDCFHEGPNFLVIDPDECIDCT
                     LCEPECPVEAIFSEEELPDDQVEYKALNAELSRSWPVITEKGEPPADAKEWEGKPGKK
                     ALLER"
     gene            complement(5194..5733)
                     /locus_tag="PKN91_09370"
     CDS             complement(5194..5733)
                     /locus_tag="PKN91_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNS28323.1"
                     /translation="MNFCSHCGAAVAYQVPPGDHLPRFVCARCGTIHYQNPKLVVGCV
                     PEDGGRILLCKRAIEPRRGYWTVPAGFMENDETLQQAAERESHEEALARVEIGSLLTI
                     VHVMHAHQVHVFFRARLAEPQFGVGPESLESKMVTEGEVPWDDLAFPSITFTLQRYFE
                     DRGARVERQHFATFDYRTR"
     gene            complement(5730..6407)
                     /locus_tag="PKN91_09375"
     CDS             complement(5730..6407)
                     /locus_tag="PKN91_09375"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HNS28324.1"
                     /translation="MNTVKMTSALCGLAAATLLLGACATPGPATSWEGLDLVAQKKFD
                     AVYLKRDADLVRYTEIMLDPLQVSFDRNWDPRPGYTSLQRADTGRIQRALADEFRKVF
                     EQTLTADGRFRLVTEAGPATLHIQPAIVDLYITAPDMSMQTAGRVVTYTVDPGRMTLA
                     ADFRDGATGTLLGRVVDEKQGVDKGYLQVTNVVTNTADARRAFQQWANAIRDGLDAVK
                     AMAPANR"
     gene            complement(6543..6629)
                     /locus_tag="PKN91_09380"
     tRNA            complement(6543..6629)
                     /locus_tag="PKN91_09380"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6593..6595),aa:Leu,seq:caa)
     gene            6757..7329
                     /locus_tag="PKN91_09385"
     CDS             6757..7329
                     /locus_tag="PKN91_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNS28325.1"
                     /translation="MAGFGSIDPGQALIVAAALGERPAREQLYLAVAPVVRAFARRMV
                     SNAAADDIMQDTVVAMFEHLGEFRGECAFGFWVRRIALTRCLMHLRSPWQRVRDAWGA
                     ALGDHEAAPGASVGELLDLQRALDALPAMSRAVLWMYEVEGFTHEEIARAFGRSVSFS
                     KSRLARAHAQLRGGAPAAAEEGTCKAITDS"
     gene            7305..7823
                     /locus_tag="PKN91_09390"
     CDS             7305..7823
                     /locus_tag="PKN91_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28326.1"
                     /translation="MQGDHRLLTLAAHEGALPAPYSFEEFERRAGQVRGQARRRERRA
                     GAIALASLMLALAGGLLHVGTKTPAPRPESARRPADLLAEPQAAPAEAMDAERWLASA
                     PSEPVVVRVGVYAAVAALEDRIAFIDDSMNDGRLAGDPQFDPASLARERARLINTLAS
                     VRYAQAVAGALN"
     gene            7844..8692
                     /locus_tag="PKN91_09395"
     CDS             7844..8692
                     /locus_tag="PKN91_09395"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNS28327.1"
                     /translation="MTRPILIPLLLSIAFAGSSAARAAGDPDLEAKLADARQRLEAAA
                     KDLAELSAEMSGPLLRNLINEGFARPVIGVQLDPEGGSEGARVTHVSPGGPAASAGMR
                     TGDVIVAVAGESMTGKPNAARAVTDALRAAAPEEPVKIRVLRDGKAQELSVVPRPLAY
                     GGRVFAGPRALTMPLPSVTPLPSGDFMFSPAMHAISGLEFVTLTPGLGEYFGADKGVL
                     VVRAPKDGALGLREGDVIREIGGREPQSSAHATRIIASYQPGESVPITVLRKKQSVAL
                     NSVLPQ"
     gene            8731..>9099
                     /locus_tag="PKN91_09400"
     CDS             8731..>9099
                     /locus_tag="PKN91_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNS28328.1"
                     /translation="MRPDDFRFDLPPGLIAQSPLPRRSASRLLTLDGATGAWADRWMR
                     DLPLLLEPGDLLVFNDTRVMPARVFGVKPTGGRVEMLLERPADGTTAWVQLRASKGFA
                     DGASFATGGGPVRVLRREDDL"
BASE COUNT         1454 a         3053 c         3150 g         1442 t
ORIGIN      
        1 ggcgcgaacc cggtgagcca gctcgcgttc acgctctcga acggcttcac gatcgtcgag
       61 tactacctgg cgcgcggcat gcgcatcgac gacttcgcgc cgaacctgtc gttcttcttc
      121 tcgaacggca tggacccgga gtacgtggtc atcggccggg tcgcgcgccg catctgggcg
      181 cgcgcgatgc gcgagcgcta cggcgcaggc ccgcgcagcc agatgctcaa gtaccacgtg
      241 cagacctcgg gccgttcgct gcatgcgcag gaaatccagt tcaacgacat ccgcacgacg
      301 ctgcaggcga tgtacgcgct gttcgacaac tgcaactcgc tgcacaccaa tgccttcgac
      361 gaggcgctga ccaccccgac cgaggacagc gtgcggcgtg cggtcgcgat ccagctgatc
      421 atcaaccgcg agctcgggct caacaggaac cagaacccgt ggcagggctc gttcatcatc
      481 gactacctga ccgacatggt ggaggaggcg gtgtatcgcg agttcgagtc gatctcggct
      541 cgcggcggcg tgctcggcgc gatggagacg atgtaccagc gcggcaagat ccaggaagag
      601 agcctgcact acgagacgaa gaagcatgac ggctcgctgc cgctgatcgg cgtgaacacc
      661 ttcctctcgg gccacgatga ctcggaggcg cgtgcgaacg tggcgctcat ccgctcgacc
      721 gaggaggaga agcaggacca ggtcgccgcg gtgcgcgcgt tccagcagcg gcatgcggcc
      781 gaagcaccgg tggcgctcgc gcgcctgcag caggtcgcgg cgggcggcgg caatgtgttt
      841 gccgagctga tggagacggt gaaggtctgc tcgctcgggc agatctcgcg cgcgctctac
      901 gaggtcggcg gccagtaccg ccgcaacgtc tagcgcccga gcgcgagccc gcgcacgtcg
      961 atgtcgggtg tgcggccggt gaccaggtcg gcgatcacgc gaccggagcc gcaggccatc
     1021 gtccagccga gcgtgccgtg gccggtgttg agccacaggt tgcgcagcgg cgaggcgctc
     1081 acgaacggcg tgccgtcggg cgtcatcgga cgcagccccg accagaactc ggcctgcggg
     1141 tagtcggcac cgcccgggaa caggctgccg accgacagct cgagcgtcgc gcgacgtctc
     1201 gggtgcagcc ggttgttgta gccggacagt tccgcggtgc cacccacgcg aatgcgatcc
     1261 ccgaggcgcg tgatcgcgat cttgtagctc tcgtccatca cggtggactg cggcgccttg
     1321 cgctcgtcga cgatgggcgc ggtgagcgag taacccttga ccggatagac gggcaggcga
     1381 aatccgagct ggcgcgccat cggcgtcgaa tagctgccga gcgcgagcac gtaggcatcc
     1441 gccttgacgg gaccggcgtc ggtgagtacc gactcgatcc ggtcgccggc gcgctcgaaa
     1501 cccaggatgc tcacgccgca gcggaaggtc acgccttgcg cctgcgccaa agcggcgagc
     1561 cgctgcgtga acaggtggca atcgccggtc tcgtcgtcgg gcaggcgcag tccgccgacg
     1621 atgaggtgcc tgacgccggc gagtccgggc tcggcgccga tgcagcccgc ggcatcgagc
     1681 agttcgtagg gcacgccgag tcgggcgagc accgcgatgt cgctggccgc gccgtcgaac
     1741 tgcgggcggg tgcgaaacag ctgcagcgtg ccgcgcgtgc gttcgtcgta gcgaatgccg
     1801 gtctgcgtgc gcagttcgcg cagcaggtcg cggctgtatt ccgcgatcgg caccatgcgg
     1861 ctcttgttga ggtcatagcg cgccgcggtg caattgcgca gcatgcgcag gatccacagg
     1921 tacatcgacg gatcgagccc agggcgcacc accagcggcg catgccgctc gaacagccac
     1981 ttgatcgcct tcagcggcat gccgggaccg gcccacggcg aggcatagcc cggggagatt
     2041 tccccggcat tcgcgaaact cgtttcgagc gcgacgcccg gctggcgctc gatcacctcc
     2101 accgcggcgc ccgcgcgcgc gaggtaccac gcggcggtga caccgatcac gccgcctccc
     2161 aggatcacta cgcgcatgac cgcacccgca gtccgttgca gccaggcatg gttacgcagc
     2221 ttctgagaga tcgggttcgc cgagcgccgc caggatgtcc cggcgggcgc aatcacgcag
     2281 cgtgacgctc gcctgctcgt cgccattctg cgccgccggc agcggcggca ggaattgcac
     2341 ctcgaggcgt gcgggcagca gtcccaggcg cttcgcgggc catgcgtcgc gcgtaccgcg
     2401 tatgacgcag ggcacgaccg ggcagccggc ccgcgccgcg gtcatgaatg cgccactgtg
     2461 gaatttcatc aggcccggct ggtgcgcaaa cgtgccctcg ggaaagaaaa cgaacgagtg
     2521 cccgttgccg gcgttgcgca ccacgcgacg cgcgtcactc gcacccttgt ggcggttgaa
     2581 gcgctcgacg aattccgagc cgatgcgcgc gaggagcagt cccgcgagcg gcagacgcga
     2641 tgcctcgcgc ttgatcacga agccgaaacg cggcggcagg gccgcggtca tcacgaaccc
     2701 atccaggtag ctggcgtggt tggccacgac cacgcactgg ccggcgggca gatggtccgg
     2761 ccgcaggatg tcgaggcgca tgccggtgag ccacaaccat gcgcgggcga tgtagtgcgc
     2821 cgtgctgcgc cgcaggcgca ttgtcggcag caacaggacg ccgagcagcg cgagcaggcc
     2881 gagcggcacg aacaccgaga gcgcgtacag cgtgtacagc caattgagcg gcgggggcag
     2941 tcgcacggcg cgggacatgt gtgaggtcat actccagctg caggaagacg gtgtgaactg
     3001 gttctcttta tgttaatgct tgctggtata tgtgaaggtg atcacgccca ccccccggcg
     3061 ggttccccaa actcgctcca aatggcacga ccagcgaccg caaacgagct gcgcaagccc
     3121 gccggtgaaa ccgacccggc ggtgacccgc ttcattgatg ccgtctggat ggagcggggc
     3181 ctgtcgtcga acacactggc agcctaccgg gccgatctta ccgcgctgtc gcgctggctg
     3241 gccgagcacg gccgcgaaat cacgcaaacg acgcggtccg acctgatgga ttttatcgcg
     3301 tttcgcgtca aggccggtgc ccggccacga tcgacggcgc gccagctgtc gtcgttccgc
     3361 cgcttcttcc gccacctcgt ccgcgagggc acgctgcggg aggatccgac cgcacagatc
     3421 gcgatgccga agatcggccg ctcgctgcca cgctcgctga gcgagggtga agtcgagtcg
     3481 ctgctgaatg cgcccgcggt ggccgatccg ctcggcaacc gcgatcgcgc catgctcgag
     3541 gtgctctatg ccacgggcct gcgcgtctcg gaactggtga gcctgcgcca cagccaggtg
     3601 aacctgaacc agggcgtgct gcgcatcgtc ggcaagggca accgcgagcg cctgattccg
     3661 ctcggcgagg aggccgtgcg ctggctgagt gagttcgtgc gcgggccgcg caacgagatc
     3721 ctgctcgatc gcaccaccga ctacctgttc ccgacccggc gcggtgatcg catgacgcgc
     3781 caggctttct ggcacatcat caagcgctat gcgcgcaagg cgggcgtcga gcgcgacctg
     3841 tcgccgcaca cgctgcgcca cgcgttcgcc acccatctgc tgaaccacgg cgcggacctg
     3901 cgcgtcgtgc agatgctgct cgggcattcc gacctgtcca cgacgcagat ctatacgcac
     3961 gtcgcgcgcg agcgcatgaa ggacctgcac gcgcagcatc atccccgggg ctgagtccgg
     4021 gctggtagac ttgtcagttg cgcccccggg gtggccgaac ctttccggcc cgcgcgggtc
     4081 gaagaggtca tcatgctgcg agccaagacc ttcctgctgc tgcttgccct gggtctcgtc
     4141 ccgttcgcgg gccacgccga tccgcgggtc gaactcgcta agaaattccc cggcgccacg
     4201 gccgaggacc tgacaccgtc gcggattccg ggcgtgtacg agctgcgtcg cggtgccgat
     4261 ctggtgtacg tcacgcccga cgggcgctac gcactgatgg gcgatctcta cgacctgcgt
     4321 gacaacagca atgtcaccga gaaccgccgc cgcgcgctgc gcgtcgcgtt gctcgaaaag
     4381 gtcccggaca ggcaggcgct gatcttctcg cccaaggatc cgaaatattc gatcgatgtg
     4441 ttcaccgaca tcgactgcgg ctattgccgc aagctgcaca gccagatcgt cgaatacaac
     4501 aagctcggcg tgcgcgtgcg ttatctgttc tacccgcgca gtggcccgaa caccgaatca
     4561 tggaagaagg ccgagaacgt ctggtgcgcg gccaatcgca acgacgcgct gacccgcgcc
     4621 aagcgcggcg agaccgtcaa gtcaccggcc tgcggtgcga cacccgtcgc gcgccagtac
     4681 gagctgggcg aggatctggg catatcgggc acgccggcga tcgtgctcgc caacggcgag
     4741 ctgctgccgg gctatgtacc gccggcggtt ctggtcgatc acctgaagac tgccgcgcgc
     4801 tgatcagcgc tcgagcagcg ctttcttgcc gggtttgcct tcccattcct tggcgtcggc
     4861 ggggggttcc cccttctcgg tgatcaccgg ccagctgcgc gacagctcgg cgttgagcgc
     4921 cttgtattcg acttggtcgt caggcagctc ttcctcggaa aaaatcgcct cgaccggaca
     4981 ttccggctcg cacagcgtgc agtcgatgca ttcgtcaggg tcgatcacca ggaagttggg
     5041 cccctcgtgg aagcagtcca ccgggcagac ttccacgcaa tccatgtatt tgcacttgat
     5101 gcagttttcg gtaacgacaa aggccatgat cgatcctggg ttcgggatcg gtattctgcc
     5161 acttatgggc cgttcagcgt agtcaacggc aggtcaacgt gtccggtagt cgaacgtcgc
     5221 gaagtgctgt cgttcgacgc gcgcgccgcg atcctcgaag tatcgctgca gggtgaacgt
     5281 gatgctggga aacgcgagat cgtcccacgg cacctcgccc tcggtgacca ttttcgattc
     5341 caggctttcc ggaccgacgc cgaactgcgg ctcggcaagg cgcgcgcgga agaacacgtg
     5401 cacctggtgg gcgtgcatca cgtgcacgat cgtgagcagg gagccgatct cgactcgcgc
     5461 gagcgcctcc tcgtgcgatt cgcgttcggc tgcctgctgc agcgtttcgt cgttctccat
     5521 gaagcccgcg ggcacggtcc agtaaccgcg ccgcggctcg atcgcgcgtt tgcacagcag
     5581 gatccgtccg ccatcctcgg gcacgcagcc caccaccagc ttcgggttct ggtagtggat
     5641 cgtgccgcac cgggcgcaga cgaagcgcgg caggtgatcg cccgggggca cctggtatgc
     5701 caccgccgcg ccgcagtggc tgcagaagtt caccggttgg cgggcgccat ggccttcacg
     5761 gcatcgagcc cgtcacggat cgcgttcgcc cactgctgaa atgcccggcg cgcatcggcg
     5821 gtattggtga cgacattggt gacctgcagg tagcccttgt ccacgccctg cttttcatcc
     5881 acgacgcgcc cgagcagcgt accggtcgcg ccgtcgcgga agtccgcagc gagcgtcatg
     5941 cgtcccggat cgaccgtata ggtcacgacg cgacccgccg tctgcatgct catgtcaggc
     6001 gcggtgatgt acaggtccac gatcgccggc tggatatgca gcgtggccgg gccggcttcg
     6061 gtaaccagac ggaatctgcc gtccgccgtc agggtctgct cgaacacttt cctgaactcg
     6121 tccgcgaggg cgcgctggat ccggccggtg tcggcgcgct gcagcgaggt gtatccggga
     6181 cgcgggtccc agttgcggtc gaacgagacc tgcagcgggt cgagcatgat ctcggtgtag
     6241 cgcaccaggt ccgcgtcgcg cttgaggtag acggcgtcga actttttctg cgcaacgaga
     6301 tcgagccctt cccagctcgt ggccgggccc ggtgtcgcgc acgcgccgag cagcagggtc
     6361 gcggcggcga ggccgcacag cgccgatgtc atcttcacgg tgttcatggt gtccgcctcg
     6421 ttgtcgggcc tttgatgcca acgatagcag acgcatggag tgcgccgcac gccgggccgc
     6481 ccaggcccga cgcgcatccg ttgcgcgcta catccgccga cgccctggcc agcggggctg
     6541 attggtgccg ggagagggaa tcgaacccac actctgtcgc cagaaccgga ttttgagtcc
     6601 ggcgcgtcta ccagttccgc catcccggcc ggaaacagcg gcgagactgt gccagaacgg
     6661 ccggtcgctg gccagaattc gaccgggcgc tggccggaaa tcgcgaaatc ggcgtcgggg
     6721 ttgcgacctt ttccgtttcc cgggcatcaa agcaatgtgg caggtttcgg cagcatcgat
     6781 cccgggcagg cgctgatcgt ggccgcagcg ctcggcgagc gcccggcgcg cgagcagctc
     6841 tacctggcgg tcgcgccagt cgtgcgggcg tttgcccgcc gcatggtttc gaacgccgcc
     6901 gcggacgaca tcatgcagga cacggtcgtc gccatgttcg agcatctcgg cgagttccgc
     6961 ggcgagtgcg ctttcggttt ctgggtgcgc cgcattgcgc tgacgcgctg cctgatgcac
     7021 ctgcgctcgc catggcagcg ggtgcgtgac gcctggggcg cggcactcgg cgaccacgag
     7081 gccgcacccg gcgccagcgt cggcgaactg ctcgacctgc agcgcgccct cgatgcactg
     7141 ccggcgatga gtcgggcggt actgtggatg tacgaggtgg aggggttcac ccacgaggag
     7201 atcgcgcgcg ccttcgggcg cagcgtcagc ttctcgaagt cgcggctggc gcgggcgcac
     7261 gcccaattgc gtggcggggc gccggcggcc gcggaggaag gcacatgcaa ggcgatcacc
     7321 gactcctgac cctggccgcc catgaaggcg cgctgccggc accgtactcg ttcgaggaat
     7381 tcgagcggcg tgccggacag gtccgcgggc aggcgcgccg gcgcgaacgg cgcgctggcg
     7441 ccattgcgct cgccagcttg atgctggcgc tggccggcgg cttgctgcat gtcggcacga
     7501 aaacgcccgc gcccaggccc gagagcgccc ggcgaccggc cgacctgctg gcagaaccac
     7561 aggcggcgcc tgcggaggcg atggatgccg agcgctggct cgcgtcggcg ccgtccgagc
     7621 cggtggtagt gcgtgtcggg gtctacgcgg ccgtagccgc gctcgaggac cgcatcgcct
     7681 tcatcgacga cagcatgaat gacggccgcc tcgcgggcga tccgcagttc gatcccgcga
     7741 gcctcgcgcg cgaacgggca cgtctgatca atacgctggc gagcgtgcgc tacgcgcagg
     7801 ccgtcgcagg tgccctgaac tgagcccaca cggagtgaag cccatgacac gtcccatcct
     7861 gatcccgttg ctcctgtcga tcgccttcgc cggcagctct gccgcgcggg cggctggcga
     7921 tcctgatctc gaagcgaaac tcgccgatgc ccggcagcga ctcgaggcgg cggcgaagga
     7981 tctcgccgag ctgtcggccg aaatgagcgg accgctgctg cgcaacctga tcaacgaggg
     8041 tttcgcgcgg ccggtgatcg gcgtacagct cgatccggaa ggcggcagcg agggcgcgcg
     8101 cgtcacgcat gtgagccccg gcgggccggc ggcgagcgcc gggatgcgca cgggcgacgt
     8161 gatcgtggcg gtggcgggcg agagcatgac cggtaagccg aacgccgcgc gcgcggtcac
     8221 ggatgcattg cgcgcggccg cgcccgagga accggtgaag atcagggtgc tgcgtgacgg
     8281 caaggcgcag gagctgtcgg tcgtgccgcg gccgctggcc tatggcggga gggtgtttgc
     8341 cggtccgcgg gccctgacga tgccgctgcc ctccgtcacg cctttgccat cgggggactt
     8401 catgttcagc cccgcgatgc atgcgatttc gggcctcgag ttcgtcaccc tcaccccggg
     8461 gctcggcgag tacttcggcg ccgataaagg tgtgctcgtg gtgcgtgcgc cgaaggacgg
     8521 tgcgctcggc ctgcgcgaag gcgacgtgat ccgggagatc ggtggacgcg agccgcagag
     8581 cagcgcgcat gcgacccgca tcatcgcctc ctaccagccg ggagagagcg tgccgatcac
     8641 ggtgctgcgc aagaagcagt cggtcgccct gaactccgtg ctgccccagt aggcgcgccg
     8701 gggcagttgg gcgcctgcta acatgcccgc gtgcgccccg acgacttccg attcgacttg
     8761 ccgcccgggc tgatcgcgca atccccgctg ccccgacgct ccgccagccg cctgctgacg
     8821 ctcgacggcg ccaccggtgc gtgggccgat cgctggatgc gtgacctgcc gctgctgctc
     8881 gagcccggcg acctgctcgt gttcaacgac acgcgcgtca tgccggcgcg agtgttcggc
     8941 gtgaagccga ccggcgggcg cgtggagatg ctgctggagc ggcccgcgga cgggacgacg
     9001 gcgtgggtgc aactgcgcgc cagcaagggc tttgccgacg gcgcgagctt cgcgacgggc
     9061 ggcgggccgg tgcgcgtcct gcggcgggag gacgacctc
//
LOCUS       DAOQVY010000098         8902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_8715_length_8902_cov_5.523567, whole genome shotgun sequence.
ACCESSION   DAOQVY010000098 DAOQVY010000000
VERSION     DAOQVY010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8902
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8715_length_8902_cov_5.523567"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PKN91_09405"
     CDS             complement(<1..723)
                     /locus_tag="PKN91_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNS28329.1"
                     /translation="MAVDIAQLLAFAVKNNASDLHLSSGVPPMIRVDGDMKRINMPAL
                     THKEVHSMVYDIMNDKQRKSYEEFFETDFSFEIPKLARFRVNAFNQNRGAGAVFRNIP
                     STIMSLDDLGAPKIFRDLCMLPRGLVLVTGPTGSGKSTTLAGMINHCNDNRPDHILTI
                     EDPIEFVHESKRSLVNQREVHRDTLGFSEALRSALREDPDVILVGEMRDLETIRLALT
                     AAETGHLVFGTLHTSSAAKTIDR"
     gene            778..1470
                     /locus_tag="PKN91_09410"
     CDS             778..1470
                     /locus_tag="PKN91_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNS28330.1"
                     /translation="MLIGPQNLPANVRNVREAIQAALLECGRSVDSVTLLAVSKGQPA
                     ESVRAAAAEGIRDFGESYLQEALPKLGQLADLDLTWHFIGQLQSNKTRAVAEHFAWVH
                     GVDRARHAERLAAQRPYHAPPLNVCIQVNVGAEVGKAGVAPGEVAPLAEAIRALPRLR
                     LRGLMCLPPEEHEVARQRHWFALLRREFAALVSSGLEIDTLSMGMSGDFVAAIHEGAT
                     IVRIGTALFGPR"
     gene            1480..2301
                     /gene="proC"
                     /locus_tag="PKN91_09415"
     CDS             1480..2301
                     /gene="proC"
                     /locus_tag="PKN91_09415"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNS28331.1"
                     /translation="MTAQQIAFIGGGNMGRALVAGLIAHGTPPAGLAVSDPSEAVRKA
                     LAADFGVRTGNDNNALAATADIIVLAVKPQGMAGVITALAPMLAQRRPLLISIAAGVR
                     TAALASWSGARLPIVRAMPNRPALVGAGITGLFATPDTPPEARKRAEAVMGAVGELVW
                     VQTEDDLDVVTAVSGSGPAYFFLLAEQLAHSAVRRGLDPHVARRLAVATLSGAGRLAA
                     DSDGDLARLRAEVTSKGGTTEAALNAFSAAGFDGIVAAAVDAAVLRGRELSESST"
     gene            2298..2828
                     /locus_tag="PKN91_09420"
     CDS             2298..2828
                     /locus_tag="PKN91_09420"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNS28332.1"
                     /translation="MNALYYIVETLISLVFIVFLLRLILQLARADFRNPIAQAIVALT
                     NPVIVPLRRILPPTGKVDTASVVAVVLVALAEIGLLYALRGFGTPDAFTWLRIAALRI
                     LTKVLWVYFYAIVLYALLSLIGPGQHTPISSLLASICEPILRPFRRLVPPIGGIDLSP
                     LWACIALQALLILLRG"
     gene            2874..3371
                     /locus_tag="PKN91_09425"
     CDS             2874..3371
                     /locus_tag="PKN91_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNS28333.1"
                     /translation="MVQTPLPPPGVRGILENKWFRHHPIAKNSLRAVMHMLCCRPNRD
                     RAARAPRDQQPNIPGEEAMAKKGGAPTKSEILAAICKETGLSRKQVSSVFDSLSGVIR
                     KSLRANGLFTLPGLLKLKVVKKPATKAREGVNPFTGEKMTFKAKPASKKVRAMPLKTL
                     KAFVN"
     gene            complement(3411..4052)
                     /locus_tag="PKN91_09430"
     CDS             complement(3411..4052)
                     /locus_tag="PKN91_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNS28334.1"
                     /translation="MAPAGRFTIDGPAGRLEVLLEDRTSAPVPAFGVVCHPHPLQGGT
                     MDNKVVFTLARALQDVGMPTLRFNYRGVGRSEGSYDEGRGETLDALAVAALGASRWPG
                     VPVWFAGFSFGGFVAINASHQLDAGAVIAVSPAVGRLASFANDGPTCPWLLVQGDADE
                     VLDPGDVLGWARTQQPAPELIVMPGVSHFFHGRLVELREHVARFARKAKNPAK"
     gene            complement(4063..4407)
                     /locus_tag="PKN91_09435"
     CDS             complement(4063..4407)
                     /locus_tag="PKN91_09435"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNS28335.1"
                     /translation="MSLVINDIKEGDGAAVGPGQAAVVHYTGWLFDGGAPEAKGEKFD
                     SSHDHGQTFTFRIGAGEVIPGWDQGVAGMKVGGQRRLVIPPELGYGARGAGGVIPPNA
                     TLVFDVELVGIQ"
     gene            complement(4542..4946)
                     /gene="msrB"
                     /locus_tag="PKN91_09440"
     CDS             complement(4542..4946)
                     /gene="msrB"
                     /locus_tag="PKN91_09440"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNS28336.1"
                     /translation="MSDDARRDELRRRLSPLAYHVTQEKGTERAFTGEYCGNKASGTY
                     SCVVCGTELFSSDTKYDSGSGWPSFWLPLAGERIAQHRDTSHGMVRIEIECARCGAHL
                     GHVFDDGPPPTGQRYCVNSASLEFKPIRGATE"
     gene            complement(4975..6861)
                     /gene="htpG"
                     /locus_tag="PKN91_09445"
     CDS             complement(4975..6861)
                     /gene="htpG"
                     /locus_tag="PKN91_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNS28337.1"
                     /translation="MTASRSETRGFQAEVRELLKLMIHSLYSHREIFLRELISNASDA
                     NDKLRFAALADPALLEGDPELSIRIETDEKAGTITVRDNGIGMTRAEAIEHLGTIAKS
                     GTAEFFSQLTGDQQKDSQLIGQFGVGFYSAFIVADEVEVFTRKAGSPAGEGVRWASKA
                     DGEFTVETVERAGRGTTIVLHLKDDARDFLDAFRLRALVRRYSDHIAFPVQMAKVGEA
                     TLDFEVVNQAKALWTRPRTEISDDEYREFYQHVAHDFEPPLAWSHNKVEGRREYTSLL
                     YVPAHAPFDLWHREGPRGLKLYVRRVFIMDDAEQFLPPWLRFIRGLVDSNDLPLNVSR
                     ELLQQDADVEAMRGALTKRALELIGKLAGDEDAGKYTTFWREFGTVLKEGVAEDAATR
                     EKLLPLLRFASTQGEGDAASVRLADYVGRMQPGQDAIYYVVADGVAAARTSPYIERLT
                     ARGIEVLLLGDRIDEWVMSRLDKFDGKPFRDAARGDLSLGKLDAGEEARHDAAVKESK
                     GLLKRIKDALGERVSEVRVSRRLADSPACLVLAEHDPGAQMRRILASAGQTPPRGKPV
                     LEVNVEHPLLQHIAGLEAGEAFDELALLLHDQAALAEAGEIANPGEFVRRLNRVLLQL
                     AALR"
     gene            complement(6895..7023)
                     /locus_tag="PKN91_09450"
     CDS             complement(6895..7023)
                     /locus_tag="PKN91_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28338.1"
                     /translation="MERLLLLWDEFDDWLGAGRHLVVMNLTGPVGWLRRHRHGSAP"
     gene            7093..7839
                     /locus_tag="PKN91_09455"
     CDS             7093..7839
                     /locus_tag="PKN91_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS28339.1"
                     /translation="MQSPQRIVVMNPKGGSGKTTIAINLASHFATRGLTPLLMDFDPQ
                     GSSMRWIRKRPPNLPEIRAIAAFERDTRLTRSFQLRVPEGTGRVIVDTPAAVPSHGFP
                     DITRDAHKIIVPVLPSDIDIHAASKCIADLLLVAKIRRGDNRIGVIANRVRRNTLIYQ
                     SLMRFLESLGIPIVATFRDSQSYVRSAELGLGIHEMKSYLVREDLDQWTPLIGWLEAP
                     PPQFAAEGSHPGVDGTQAGPAVTGAAPAAG"
     gene            complement(7843..8073)
                     /locus_tag="PKN91_09460"
     CDS             complement(7843..8073)
                     /locus_tag="PKN91_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28340.1"
                     /translation="MKTPGGPRGEIPVAAGATPTPTPTRAAASEPYANWSERMQRARG
                     RHSAITRNLYTWSSYKNWAERVKNDWDDGSEK"
     gene            complement(8177..8590)
                     /locus_tag="PKN91_09465"
     CDS             complement(8177..8590)
                     /locus_tag="PKN91_09465"
                     /inference="COORDINATES: protein motif:HMM:NF023320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3412 domain-containing protein"
                     /protein_id="HNS28341.1"
                     /translation="MPGDPAEAARQMLIALEEVREYRRSVNDSYNFNWLLRVAPDFQL
                     PFEVTHASMRALELHRDLPPHVLAANLRRAFSGIVTGNVKDHGIKAIEAHGPYELSGD
                     PEVMRLLDDLLRAFVAQGRMKLPGSEYQPCYRLVP"
     gene            complement(8657..>8902)
                     /locus_tag="PKN91_09470"
     CDS             complement(8657..>8902)
                     /locus_tag="PKN91_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28342.1"
                     /translation="FEAAGYASAHAPSMEMARIGGKVIIIGISSDDRVSFRESFARRK
                     GLTIYMARRSRFALRRGIDLIVVEAGHVAHLVHDQTA"
BASE COUNT         1431 a         2997 c         2966 g         1508 t
ORIGIN      
        1 gcggtcgatg gtcttggccg ccgagctcgt gtgcagcgtg ccgaacacga ggtggccggt
       61 ctcggcggcg gtgagcgcga ggcggatcgt ctcgaggtcg cgcatttcgc cgaccaggat
      121 cacgtcgggg tcttcgcgca gcgccgagcg cagcgcttca ctgaagccga gcgtgtcgcg
      181 atgcacctcg cgctggttca ccagcgagcg cttggattcg tggacgaact cgatcggatc
      241 ttcgatcgtg aggatgtgat ccggccggtt gtcgttgcag tggttgatca tgccggcgag
      301 ggtcgtcgac ttgcccgacc cggtcggacc ggtgaccagc accaggccgc gcggcagcat
      361 gcacaggtcg cggaagatct tcggcgcgcc gagatcgtcg agcgacatga tggtcgaggg
      421 aatattgcgg aacacggcgc cggcgccgcg gttctggttg aaggcgttga cacggaagcg
      481 cgccagcttc gggatctcga acgaaaaatc ggtctcgaag aattcctcgt acgacttgcg
      541 ctgcttgtcg ttcatgatgt catacaccat gctgtgcact tccttgtggg tgagtgccgg
      601 catgttgatg cgcttcatgt cgccgtcgac acggatcatg ggaggcacgc ccgacgacag
      661 gtgcaggtcg gaagcgttgt tcttgacggc aaacgcaagc agttgcgcga tatcgacggc
      721 catgcgaagt cctcgcggag agcggtcgca gtatagcgga gggggtctgc gtccaatatg
      781 ttaatcggtc cgcagaattt gcctgcgaat gtccggaatg tgcgcgaagc gatacaggca
      841 gccctgctcg agtgcggacg aagtgtcgac tcggtcacgt tgctcgccgt ttcgaagggc
      901 cagcccgccg aaagcgtgcg tgccgccgcc gcggagggca tccgggactt cggggagagt
      961 tacctgcagg aggccctgcc gaagctcggg cagctcgccg atctcgacct cacctggcac
     1021 ttcatcgggc agttgcagag caacaagacg cgcgcggtgg ccgagcactt tgcgtgggtc
     1081 cacggcgtcg atcgcgcccg gcacgccgag cgcctggccg cgcagcgccc ctaccacgcc
     1141 ccaccgctca atgtctgcat ccaggtgaac gtgggcgccg aggtcggcaa ggccggcgtg
     1201 gccccgggtg aggtcgcgcc gcttgccgag gcgatccgcg cactgccgcg gctgcgcctg
     1261 cgcgggctca tgtgcctgcc gccagaggag cacgaggtgg cgcgccagcg tcactggttc
     1321 gcgctcctgc gccgcgagtt cgccgcgctc gtatcctccg gcctcgagat cgacacgctc
     1381 tcgatgggca tgagcggcga cttcgtcgcg gcgatccacg aaggcgcaac catcgtgcgg
     1441 atcggcaccg cgctgttcgg tccacggtaa gatcggcgca tgacagcaca gcagatcgca
     1501 ttcatcggcg gcggcaacat gggccgagcg ctcgtcgccg gcctgatcgc acacggcacc
     1561 ccgcccgccg ggctggccgt gtccgatccg agcgaagcgg tgcgcaaggc gctggcagcc
     1621 gatttcggcg tgcgcaccgg caacgacaac aacgcattgg ccgccaccgc cgacatcatc
     1681 gtgctcgccg tgaagcccca ggggatggcc ggcgtgatca ccgcactcgc gccgatgctg
     1741 gcgcagcgcc gaccgctcct catctcgatc gccgcgggcg tgcgcaccgc cgcgctcgcg
     1801 agctggagcg gcgcccggct gccgatcgta cgcgcgatgc ccaatcgccc cgcgctggtc
     1861 ggcgccggca tcacgggcct ctttgcgaca cccgataccc cgcccgaggc gcgcaagcgc
     1921 gccgaagccg tcatgggcgc ggtgggcgaa ctggtctggg tccagaccga ggacgacctc
     1981 gacgtggtga ccgccgtgtc gggcagcggg ccggcctact tcttcctgct cgccgaacaa
     2041 ctggcgcaca gcgccgtgcg ccgcggcctc gatccccacg tcgcgcgacg gctcgccgtg
     2101 gcaaccctga gcggagcggg ccgtctggcc gcagacagcg acggcgacct cgcccgactc
     2161 cgtgccgagg tcacctccaa aggcggcacc accgaggcgg cactcaatgc cttctcggcc
     2221 gccggtttcg acgggatcgt cgccgccgcg gtcgatgcgg cggtgttgcg cggacgcgaa
     2281 ttgtcggaga gcagcacttg aacgccctct actacatcgt cgagaccctg atctcgctcg
     2341 tattcatcgt cttcctgctg cgactcatcc tgcagctcgc gcgcgccgac ttccgcaatc
     2401 ccatcgcgca ggcaatcgtc gcgctcacca acccggtgat cgtgcccctg cgccgcatcc
     2461 tgccgcccac cggcaaggtc gacacggctt cggtcgtcgc ggtagtgctg gttgcactcg
     2521 ccgagattgg tctcctctac gcgttgcgcg gcttcggcac gcccgatgcc ttcacctggt
     2581 tgcgcatcgc ggcactgcgc atactgacca aggtgctgtg ggtctacttc tacgcgatcg
     2641 tgctgtatgc actgctctcg ttgatcggcc cggggcagca cacgccgatc tcttcgctgc
     2701 tcgcatcgat ctgcgagccg atcctgcggc ctttccggcg cctcgtaccg ccgatcggcg
     2761 gcatcgacct gtcgcctctc tgggcgtgca tcgcccttca ggccttgctg atactgttac
     2821 gaggatgatc gagaacatca ttgagttagc tgacgttatg cgtcgcaatt cgcatggtgc
     2881 agacgccgct cccgccacct ggtgtgcgcg gaatactgga aaacaagtgg tttcggcacc
     2941 acccgatcgc gaaaaatagc ttgcgcgcgg taatgcatat gctatgttgc cgcccgaatc
     3001 gcgatcgcgc ggctcgcgcg ccgcgtgatc agcaaccaaa tattccagga gaagaagcaa
     3061 tggcgaaaaa aggtggcgca ccgacgaagt cggaaatcct cgcagcgatc tgcaaggaaa
     3121 cgggtctgtc ccgcaagcag gtttcgtccg tgttcgattc gctgtcgggg gtgattcgca
     3181 agagcttgcg cgccaacggg ctgtttacgc tgcccggcct gctgaagctg aaagtcgtga
     3241 agaagcccgc caccaaggcg cgcgagggcg tgaatccctt cacgggcgag aagatgacct
     3301 tcaaggccaa gccggcctcg aagaaggtcc gtgcgatgcc gctgaagacc ctcaaggcct
     3361 tcgtgaactg agttttcgcc tgcttttggc gccagaaagc ccggcgaggg tcacttcgcc
     3421 gggttttttg ctttccgggc gaagcgggcg acatgttctc gcagttcgac cagcctgccg
     3481 tggaagaaat gcgacacgcc gggcatcacg atgagctcgg gcgccggctg ctgggtgcgc
     3541 gcccagccga gcacgtcgcc cggatcgagc acttcatcgg catcaccctg taccagcagc
     3601 cacggacatg tcggtccgtc gtttgcgaag ctggcaagcc tgccgacggc cggcgagacg
     3661 gcgatcaccg cgccggcatc aagttgatga gaggcattga tcgccacgaa gccgccgaag
     3721 gagaagcctg cgaaccacac gggcacgcca ggccagcgtg aggcgcccag ggcggcgacc
     3781 gcgagcgcat cgagggtctc gccccgccct tcatcgtagc tgccctcgct ccgccccacg
     3841 ccgcggtaat tgaagcgcag cgtgggcatg ccgacatcct gcagtgcacg tgcgagggtg
     3901 aatacgacct tgttgtccat cgtgccgccc tgcagcgggt gcggatggca cacgacgccg
     3961 aaggccggca cgggagcgct cgtgcgatcc tcgaggagca cctcgaggcg gcccgcgggg
     4021 ccgtcaatgg tgaaacgtcc ggcgggcgcc atccgcgcgc cgttactgga tgcccaccag
     4081 ttcgacgtcg aagaccagcg tggcgttcgg ggggatcacg ccaccggcgc cgcgcgcacc
     4141 gtagccgagc tcgggcggaa tgacgaggcg gcgctgcccg ccgaccttca tgccggcgac
     4201 gccctggtcc cagccgggaa tcacttcacc ggccccgatg cgaaacgtga aggtctggcc
     4261 atgatcgtgc gagctgtcga acttctcgcc cttggcctcg ggtgcacccc catcgaacag
     4321 ccagccggtg taatgcacga ccgccgcctg gcccgggccc acggccgcgc cgtcgccttc
     4381 cttgatatcg ttgatgacga ggctcatggc gggggactcc tgtactgcgg tgacggggac
     4441 gggcgcttcg gcgggtgctg cacccgcggg agtgccatcg gatgactggc tgcaggcggc
     4501 aagcgccagc gaagcaagca gcggggcggt gtaacggcgg atcattcagt ggctcccctg
     4561 atgggtttga actcgagcga cgcggagttg acgcagtagc gctggccggt cggcggcgga
     4621 ccatcgtcga acacgtggcc gaggtgcgcg ccgcaccggg cgcactctat ctcgattcgc
     4681 accatgccgt ggctcgtgtc gcgatgctgg gcgatacgct cgccggcgag tggcagccag
     4741 aaactcggcc agcccgaacc cgagtcgtac ttggtgtcgg aactgaagag ctcggtgccg
     4801 cacaccacgc aactgtaggt gccactggcc ttgttgccgc agtactcgcc ggtgaaagca
     4861 cgctcggtgc ccttctcctg cgtcacgtgg taggcgagcg gcgagagtct cctgcgcagc
     4921 tcatcccgcc gggcgtcgtc gctcatggcc tggctcccgc gaaaaggggc ggcattatcg
     4981 cagggcggcc agctgcaaca atacccggtt cagtcggcgc acgaattcgc ccggattggc
     5041 gatttctccg gcctcggcga gcgcggcctg gtcgtgcagc agcagcgcga gctcgtcgaa
     5101 cgcctcgccc gcctcgagcc ctgcgatgtg ctgcagcagc gggtgttcca cgttgacctc
     5161 gagcaccggc ttgccccggg gcggcgtctg tccggcgctc gcaagaatgc ggcgcatctg
     5221 cgcgcccgga tcgtgttcgg cgagcacgag gcaggcgggc gaatcggcga ggcggcggct
     5281 cacgcgcacc tccgaaaccc gctcgccgag cgcgtccttg atgcgcttca gcaggccctt
     5341 gctctccttg accgccgcat cgtgccgggc ttcctcgccg gcatcgagct tgcccaggct
     5401 gaggtcgcca cgcgcggcgt cccggaaggg cttgccgtcg aacttgtcga ggcgcgacat
     5461 gacccactcg tcgatgcggt ctcccagcag cagcacttcg atgccccggg ccgtgagccg
     5521 ctcgatgtag gggctggtgc gcgccgcggc gacgccgtcg gcgaccacgt aatagatcgc
     5581 gtcctggccg ggctgcatgc gcccgacgta gtcggcgagc ctgacgctcg ccgcgtcgcc
     5641 ctcgccctgc gtgctcgcaa aacgaaggag tggcagcagc ttctcgcgcg tggccgcatc
     5701 ttccgcgaca ccctccttga gcacggtgcc gaactcgcgc cagaaggtcg tgtacttgcc
     5761 cgcgtcctcg tcgccggcca gcttgccgat cagctcgagt gcgcgcttcg tcagtgcgcc
     5821 gcgcatcgcc tcgacgtcgg catcctgctg cagcagttcg cgcgatacgt tgagcggcag
     5881 gtcgttcgaa tcgaccagcc cgcggatgaa gcgcagccag ggcggcagaa actgctcggc
     5941 atcgtccatg atgaacacgc gccgcacgta gagcttgaga ccccgcggcc cctcgcggtg
     6001 ccacaggtcg aacggcgcgt gcgcaggcac gtagagcagg ctcgtgtact cgcgccggcc
     6061 ttcgaccttg ttgtggctcc aggcgagcgg cggctcgaaa tcgtgcgcca cgtgctggta
     6121 gaactcgcgg tattcgtcgt cgctgatttc ggtgcgcggc cgggtccaca gcgccttggc
     6181 ctggttcacg acctcgaaat cgagcgtggc ctcgcccact ttcgccatct gcaccgggaa
     6241 ggcgatgtgg tcggaatagc gccgcaccag cgcccgcagc cggaaggcgt cgaggaaatc
     6301 gcgcgcgtcg tccttcaggt gcagcacgat ggtcgtgccg cggccggcac gctcgacggt
     6361 ctcgaccgtg aactcgccgt cggccttcga cgcccagcgc acgccctcgc ctgcgggcga
     6421 gcccgccttg cgggtgaaga cttcgacctc gtcggcaacg atgaaggccg aatagaagcc
     6481 gacgccgaac tggccgatca gctgcgagtc tttctgctgg tcgcccgtca gctggctgaa
     6541 gaactccgcg gtgccggatt tcgcgatcgt gccgaggtgc tcgatcgcct cggcgcgcgt
     6601 catgccgatg ccgttgtcgc gcacggtgat ggtgcccgcc ttctcgtccg tctcgatccg
     6661 gatcgagagt tcggggtcgc cctcgagcag cgccggatcg gccagcgcgg cgaagcgcag
     6721 cttgtcgttg gcgtcggagg cgttcgagat cagttcgcgg aggaagattt ccctgtggct
     6781 gtacagggaa tggatcatga gcttcagcag ctcacggact tcggcctgga agccgcgggt
     6841 ttcgcttcgg gatgcggtca tggcgcgggg aatttagtgc cccgcgcggg aaaatcaagg
     6901 ggcgctgccg tgcctgtggc ggcgcagcca gcccacgggc ccggtcaggt tcataacgac
     6961 gaggtggcga cctgcgccga gccagtcgtc gaattcgtcc cacagcagga gcaggcgttc
     7021 catgaaagaa agactacgca tgccggcccc tccctcgccg tgcgcggcct cacagtgtag
     7081 gctccgcaaa ggatgcagag tccacaacgc atcgtcgtca tgaacccgaa gggcggatcg
     7141 gggaagacga ccattgcaat caacctggcc agccatttcg ccacgcgcgg cctgacaccc
     7201 ctgctgatgg acttcgatcc gcagggctcc tcgatgcgct ggatccgcaa gcgcccgccc
     7261 aacctgccgg agatccgcgc aatcgccgca ttcgagcgtg acacgcgtct cacccgcagc
     7321 ttccagctgc gcgtgcccga aggcacgggc cgcgtgatcg tcgacacccc ggccgccgtg
     7381 ccgtcgcacg gcttccccga catcacgcgc gatgcccaca agatcatcgt gccggtgctg
     7441 ccctccgaca tcgacatcca cgccgcgtcc aagtgcatcg ccgacctgct gctggtggcg
     7501 aaaatccgcc gcggcgacaa ccgcatcggc gtgatcgcca accgcgtgcg ccgcaacacg
     7561 ctcatctacc agtcgctgat gcgcttcctg gaatcgctcg ggatcccgat cgtcgcgacc
     7621 ttccgtgatt cgcagagtta tgtgcgcagc gccgagctgg ggctgggcat ccacgagatg
     7681 aagagttatc tggtgcgtga ggacctggac cagtggaccc cgctgatcgg ctggctcgaa
     7741 gcgccgccgc cgcagttcgc cgcggaagga tcgcatcccg gggttgatgg cacccaggcc
     7801 gggcccgcgg tcacgggcgc cgcaccggcc gccggctgac gcctatttct cgctgccgtc
     7861 gtcccagtcg ttcttcacgc gctcggccca gttcttgtag ctcgaccacg tgtagaggtt
     7921 gcgggtgatc gcggaatggc ggccgcgggc gcgctgcatg cgctcggacc agttcgcgta
     7981 gggctcggac gccgcagcac gcgtcggcgt cggcgtgggc gttgcgcccg ccgccaccgg
     8041 aatctcgccc ctcggtccac caggagtttt catgacattc atccgcagct tctggggcga
     8101 gcgtagggaa cgcgcgcgcg catcgacagg aactgcgtca cggcattgcg tgccgcggtg
     8161 gcacgcaggc gttatgtcat gggacgagcc ggtaacacgg ctggtattcg ctgcccggca
     8221 gcttcatgcg gccctgcgcg acgaacgcgc gcagcaggtc atcgagcagg cgcatcactt
     8281 ccggatctcc gctcagctcg tagggcccgt gggcctcgat ggccttgatg ccgtggtcct
     8341 tcacgttgcc ggtgacgatg ccggagaacg cgcgccgcag gttcgccgcg agcacgtgcg
     8401 gcggcaggtc gcgatgcagt tcgagcgcgc gcatcgaggc atgcgtgacc tcgaagggca
     8461 gctggaagtc gggcgccacg cgcagcaacc agttgaagtt gtaggaatcg ttgacgctgc
     8521 ggcggtactc gcgcacttcc tccagggcga tcagcatctg ccgtgcggct tcggccgggt
     8581 cgcccggcac gatcttcagg cgcgcacgcg ctgacctgcc gagggtgccc tcgatgaact
     8641 gcgcgatgcg ctcgaatcac gcggtttgat cgtggacgag gtgcgcgacg tgaccggctt
     8701 ccacgacgat cagatcgatc ccgcgccgca acgcgaaccg gctcctcctc gccatgtaaa
     8761 tcgtcagccc tttgcgccgc gcaaagcttt ctcgaaacga aacgcgatcg tccgaactga
     8821 tcccgatgat gatgaccttt ccgccgatgc gggccatctc catcgaaggg gcgtgggcgg
     8881 aggcgtagcc cgccgcctcg aa
//
LOCUS       DAOQVY010000099         8885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_8739_length_8885_cov_6.247225, whole genome shotgun sequence.
ACCESSION   DAOQVY010000099 DAOQVY010000000
VERSION     DAOQVY010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8885
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8739_length_8885_cov_6.247225"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PKN91_09475"
     CDS             complement(<1..378)
                     /locus_tag="PKN91_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HNS28343.1"
                     /translation="MSAHDVKSAQRPDPDQVIVDIAGYAKDQSIDSKIAYDTARYCLM
                     DTLACGFQALAYPACTRLMGPVVPGATMPGGARVPGTSYELDPVQAAFNIGAMIRWLD
                     FNDTWLAAEWGHPSDNLGGILSVA"
     gene            complement(434..>543)
                     /locus_tag="PKN91_09480"
     CDS             complement(434..>543)
                     /locus_tag="PKN91_09480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28344.1"
                     /translation="SAHVIEQRADGKIIRPTANYVGPDNRPFVPIDARP"
     assembly_gap    544..553
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<554..1603)
                     /gene="prpC"
                     /locus_tag="PKN91_09485"
     CDS             complement(<554..1603)
                     /gene="prpC"
                     /locus_tag="PKN91_09485"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HNS28345.1"
                     /translation="MADPTSATGPKPKKSVALSGVVAGNTALCTVGRSGNDLAYRGYD
                     ILEIAQTCEFEEIAHLLIHERLPNRAELAAYKRKLKDLRGLPAVVRATLETLPASSHP
                     MDVLRTGVSALGCAMPEKDDHGAAGARDIADQLLASLGSMLCYWYHYSHSGHRVHVET
                     QDDSIGGHFLHLLHRKAPPESWVRAMHTSFILYAEHEFNASTFTARVIAGTGSDLHSC
                     VTGAIGALRGPKHGGANEVAFEVQQRYASPDEAEADIRRRVAAKEVIIGFGHPVYTIA
                     DPRNQVIKSVARDLAQEARNTKMYDIAERLERVMWDAKKMFPNLDWFSAVSYHLMGVP
                     TAMFTPLFVIARTAGW"
     gene            complement(1596..2489)
                     /gene="prpB"
                     /locus_tag="PKN91_09490"
     CDS             complement(1596..2489)
                     /gene="prpB"
                     /locus_tag="PKN91_09490"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HNS28346.1"
                     /translation="MSTVSSAGARLRAAIDAEHPLQVVGTVNAYSALLAARAGFRAIY
                     LSGAGVANASFGLPDLGITSLNDVCEDVRRISSATDLPLLVDADTGWGAAFNIARTSR
                     DLIRSGAAGMHLEDQVQAKRCGHRPGKALVSAPEMADRIKAAVDGRTDAAFVIMARTD
                     AHAVEGQAAALERAQRYVEAGADMIFAEALTSLDEYREFTRVVKVPVLANITEFGKTP
                     LFTTRELGDAGVRLVLYPLSAFRAMSRTALEVYGAIRRDGSQQAVIDRMQTRAELYDV
                     LGYHDYERKLDALFGQEKGHG"
     gene            complement(2564..3550)
                     /locus_tag="PKN91_09495"
     CDS             complement(2564..3550)
                     /locus_tag="PKN91_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNS28347.1"
                     /translation="MKFKAYRIHEEDRKVVARFEQLGLDDLSPGEVVVRVSHSGINYK
                     DALAATGAGRILRRYPLVGGIDLAGTVESSVDPRVAPGDAVLVTGCGLSETVDGGYAQ
                     FARVAGDSVIPMPPGLDAVSAMTLGTAGFTAALAIHRMEHNGQTPAKGPVVVTGATGG
                     VGSIAIDMLAGRGYEVIAVSGKAQQVDYLRALGASEVLLRADIDYGSKPLEAERFAGA
                     IDNVGGETLTWLTRTVGFWGNIASIGLAGSPKLETTVLPFILRGVNLLGINSSATLRE
                     LRLKVWERIGTDLAPRHLDRIRSRIIAFDELPGAFAALLEGGVVGRTVVAIR"
     gene            complement(3569..4504)
                     /locus_tag="PKN91_09500"
     CDS             complement(3569..4504)
                     /locus_tag="PKN91_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS28348.1"
                     /translation="MRGPDDITAIDAGYVRPQMAAIHVIEHRGRAALVDTGTAHSVPR
                     VLAALASLGREPADVDYVLLTHVHLDHAGGAGAFMRALPNATLVVHPRGAPHMIDPAK
                     LIAASIEVYGESRYRELYGEIVPIDAARVHVTQDGEQLDLAGRVLEFWFTPGHAMHHQ
                     AIADPASRTVFTGDTFGLSYREFDTARGAFIFPTTTPTQFDPQQLAASVARIAAYAPR
                     AVHLTHYARVEDVDRLAADMTTLVHAFAAIAQRHAAAADRRERIRADLRALLRERSLA
                     HGCTMDEAGFDALLADDLDLNADGLVAWLERLARA"
     gene            complement(4511..5284)
                     /locus_tag="PKN91_09505"
     CDS             complement(4511..5284)
                     /locus_tag="PKN91_09505"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNS28349.1"
                     /translation="MTRKLAAMLMIAVLGGGATFAAASSEADLVLVAGATGRTGVHIV
                     TQLVDQGYRVRALVRDEAKARATLPQSAEFVVGDVRDPATLAPAMKGVTRVISTIGGG
                     ALRPVPGNGPDEIDRQGNINLVDAAKAAGVTQFVLVSSSGVDHAETYPAPFMRPILAA
                     KRASEDYLRASGLPYTIVRPGGLRDAPGEHTRIVLRQKVGAEVSWVARADVARVCIAA
                     LGNEAALGKTFDVSAVAGEPVGDLAPLFAALSADPVPAK"
     gene            5318..7162
                     /locus_tag="PKN91_09510"
     CDS             5318..7162
                     /locus_tag="PKN91_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNS28350.1"
                     /translation="MQRSYTALACAAVSALVAMATLPAHAAREERGNLTFDGVIQASP
                     ALVERITPWLAGRSATVAGWLPDGALLVTTRFGEIAQLHRVAAPLGAREQLTFGDDPV
                     IAAASSAASGAAGVAYVTAGPGAVPQLRALRFADRSTPRIAAPRVQPDALTWSPDGRT
                     VAFQAASADGVHQEILAFEPGGNLQPRLLVAAGIKSWRPLAWSPDGGRLLIQDEANSQ
                     HSELWFADVNTGGVTAIALPTPPRRVVAARFAPDGRNVYLIAQFDEFARLHRLDPATS
                     QLTTITDNVPWDIESFAASADGRYLAFVANAAGVSRLSVIDQIARMELAPQGLPEGRI
                     ADLAFDPAGRRLALTAEGATEPADAWVYEPASNTVARWTRSELGPIDRAALVAPEFIS
                     FPGWDRANGSTRALPAWVYRPRTPGPHPVLVRIASGPNEQYRPGFDAFTQFAVNVLGY
                     AVIAPNVRGAPGYGRSFEMLGESVRREDAVRDIGSLLVWIGVQRDLDRSRVVLMGESQ
                     GAQVALGSLAQYGDRVAGGISLAGMTNYSRIPNARALRRPLLVVQGAQDLVAPAFEAE
                     RLVTTVRGNGAEVWYVLAKEEAHVFRRKANRDAWMSVAAAFLQRLSGS"
     gene            complement(7185..7817)
                     /locus_tag="PKN91_09515"
     CDS             complement(7185..7817)
                     /locus_tag="PKN91_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNS28351.1"
                     /translation="MSLASRTATAFALATFLAGSVGVAHAQGNAERGKVLAQTCLGCH
                     AVENYKNSYPTYPVPKLRGQHAEYLEVALRGYQAGERSHATMHAHAASLSEQEIADIA
                     AFLGGEAVQPTGKPAAANAPEAAAVCVACHGNDGVGVLPAYPTLTGQHIEYLKRALTD
                     YKSGARKNPIMAGFVATLSAKDIEALAAYYGKMGPGLETVELRKSRFSAR"
     gene            7922..8368
                     /gene="dtd"
                     /locus_tag="PKN91_09520"
     CDS             7922..8368
                     /gene="dtd"
                     /locus_tag="PKN91_09520"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNS28352.1"
                     /translation="MIALIQRVNFAEVTVEGRLVGRIGRGILALIGVQALDTPACGSR
                     LLERVLGYRVFPDEDGRMNRSLADTGGGLLLVPQFTLAADTRKGARAGFSTAAAPEAA
                     QALFGQFVAEARRRHPEVATGEFGAHMRVALENDGPVTFWLEAHAG"
     gene            8460..8696
                     /gene="tatA"
                     /locus_tag="PKN91_09525"
     CDS             8460..8696
                     /gene="tatA"
                     /locus_tag="PKN91_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNS28353.1"
                     /translation="MRELIIILLVVLLVFGAKKLRTIGSDLGAAVRGFKKSVSEGEAE
                     DSADEVKQIKSAEKDAEFPEVAKSDTDSKINRGA"
BASE COUNT         1353 a         3000 c         3127 g         1395 t
ORIGIN      
        1 cgccaccgag aggatgccgc cgagattgtc ggacgggtgg ccccactcgg ccgcgagcca
       61 ggtgtcgttg aagtcgagcc agcggatcat cgcgccgatg ttgaacgcgg cctgcaccgg
      121 atcgagttcg tagctcgtgc ccggaacccg cgccccgccc ggcatggtcg cgcccggcac
      181 caccggtccc atcaaccgcg tgcaggccgg ataggccagc gcctggaagc cgcaggcgag
      241 cgtatccatc aggcagtagc gcgcggtgtc gtaggcgatc ttcgagtcga tcgactggtc
      301 cttcgcgtag cccgcgatgt cgacgatcac ctggtcgggg tcggggcgct gggcggattt
      361 gacgtcgtgt gcggacatgg acgggcctcg ggaaattcag taatggtgta tcagggttcg
      421 tgcgcgcgcc ggctcacggc cgcgcgtcga tcggcacgaa gggacgattg tcgggtccca
      481 cgtagttggc ggtcgggcgg atgatcttgc cgtccgcgcg ctgctcgatc acgtgcgcgg
      541 accnnnnnnn nnnccagccg gcggtgcgcg cgatcacgaa cagcggcgtg aacatcgcgg
      601 tcggcacgcc catcaggtga tacgagacgg cgctgaacca gtcgaggttc gggaacatct
      661 tcttcgcgtc ccacatcacc cgctcgaggc gctcggcgat gtcgtacatc ttcgtgttgc
      721 gcgcttcctg cgcgaggtcg cgggcgaccg acttgatcac ctggttgcgc ggatcggcga
      781 tcgtgtagac ggggtgtccg aagccgatga tgacttcctt cgcggccacg cgccggcgga
      841 tgtcggcctc ggcctcgtcc ggcgaggcgt agcgctgctg cacctcgaac gcgacctcgt
      901 tcgcgccgcc gtgcttcggt ccgcgcaacg cgccgattgc gccggtgacg cacgagtgca
      961 ggtcggaacc cgtcccggcg atcacgcgtg cggtgaaagt cgaggcattg aactcgtgct
     1021 cggcgtagag gatgaaggag gtgtgcatcg cgcgcaccca tgactcgggc ggcgccttgc
     1081 gatgcagcag gtgcagaaag tggccaccga tcgaatcgtc ctgcgtttcg acatgcacgc
     1141 gatggcccga gtggctgtag tggtaccagt agcacagcat ggagccgagc gaggcgagca
     1201 gctgatcggc gatatcgcgg gccccggcgg caccgtggtc gtccttctcc ggcatcgcgc
     1261 aaccgagcgc cgagacaccg gtacgcagca cgtccatcgg atggctcgac gcgggcagcg
     1321 tctcgagcgt ggcgcgcacc acggccggca ggccgcgcag gtccttgagc ttgcgcttgt
     1381 aggccgcgag ttcggcgcgg ttcggcagtc gctcgtggat cagcagatgc gcgatctcct
     1441 cgaattcgca ggtctgcgcg atttcgagga tgtcgtagcc acgataggcc aggtcgttgc
     1501 cgctgcgccc gacggtgcac agcgcggtgt tgccggccac cacgcccgag agcgcgacgg
     1561 acttcttcgg cttcggaccg gttgcggagg tcgggtcagc catggccttt ctcctgccca
     1621 aagagcgcat cgagtttgcg ctcgtaatcg tgatagccga gcacgtcgta gagttcggcg
     1681 cgggtctgca tgcggtcgat caccgcctgc tgcgagccgt cccggcggat tgcgccgtac
     1741 acctcgagcg ccgtgcggct catcgcgcgg aaggccgaca gcggatagag caccagccgc
     1801 acgcccgcgt cgccgagttc ccgggtcgtg aacagcggcg tcttgccgaa ctcggtgatg
     1861 ttggcgagca ccggcacctt cacgacgcgc gtgaactcgc ggtactcgtc gaggctggtc
     1921 agcgcctcgg cgaaaatcat gtcggcgccc gcctcgacgt agcgctgcgc gcgctcgagc
     1981 gccgcggcct ggccctcgac ggcatgcgcg tcggtgcgcg ccatgatcac gaaggccgca
     2041 tcggtgcggc cgtcaaccgc cgccttgatg cgatcggcca tctcgggcgc ggaaacgagc
     2101 gccttgccgg gccgatggcc gcagcgcttt gcctgcacct ggtcctcgag atgcatgcct
     2161 gcggcgccgg agcggatcag gtcgcggctc gtgcgtgcga tattgaacgc tgccccccag
     2221 cccgtgtcgg cgtcgacgag cagcggcagg tcggtcgccg agctgatgcg gcgcacgtcc
     2281 tcgcagacgt cgttcagcga ggtgatgccg aggtccggca ggccgaacga ggcgttcgcg
     2341 actcccgcgc ccgacaggta gatcgccctg aacccggcgc gcgcggcaag cagcgcggaa
     2401 taggcgttga cggtgccaac gacctgcagc ggatgttcgg cgtcgatggc ggcacgcagg
     2461 cgcgcgccgg ctgaggaaac tgtggacatt cacgcacctg cctgtagcgg accgggaacc
     2521 cgcaattgta gcggatcacg agcccgccgc gggcttcggg cggtcagcgg atggcgacga
     2581 ctgtcctgcc gacgacaccg ccctcgagca gcgccgcgaa ggcgcccggc agttcgtcga
     2641 acgcgatgat ccgcgagcgg atccggtcga ggtggcgcgg cgcaaggtcg gtgccgatgc
     2701 gctcccagac cttgagccgc aactcgcgca gcgtggccga cgaattgatg ccgagcaggt
     2761 tcacaccgcg caggatgaac ggcagcacgg tggtctcgag cttcgggctg ccggcgaggc
     2821 cgatgctggc gatgttgccc cagaaaccga cggtgcgcgt gagccaggtg agcgtctcgc
     2881 cgcccacgtt atcgatcgcg ccggcgaagc gctcggcctc gagcggcttc gagccgtaat
     2941 cgatgtccgc gcgcagcaga acctcggacg caccgagcgc gcgcaggtaa tcgacctgtt
     3001 gcgccttgcc gctcaccgca atcacctcgt agccgcgacc ggcgagcatg tcgatcgcga
     3061 tgctgccgac accgccggtt gcgccggtca cgaccaccgg cccctttgcc ggtgtctgac
     3121 cgttgtgttc catgcgatgg atcgcgaggg cggcggtgaa ccccgccgtg ccgagcgtca
     3181 tggcgctcac cgcatcgagc ccgggaggca tcggaatcac cgaatcgccc gcgacccgcg
     3241 caaactgcgc atagccaccg tcgactgttt ccgacagtcc gcagccggtg acgagcacgg
     3301 cgtcgccggg cgcaacgcgc ggatcgaccg acgattccac ggtgcctgcg agatcgatgc
     3361 cgccgacgag cggatagcgg cgcaggatgc gccccgcgcc ggtcgcggcc agcgcgtcct
     3421 tgtagttgat gcctgaatgg gaaacgcgca ccaccacctc gccgggcgag agatcgtcga
     3481 ggcccagctg ctcgaagcgc gcgacgacct tgcggtcctc ttcgtggatg cggtacgcct
     3541 tgaatttcat tgccgggatc atagcgcctc aggcacgcgc gagacgctcg agccaggcaa
     3601 cgaggccatc ggcgttgaga tcgagatcgt cggcgagcag cgcgtcgaag cctgcctcgt
     3661 ccatcgtgca gccgtgcgca aggctgcgct cccgcagcag cgcgcgcagg tcggcgcgta
     3721 tgcgctcgcg tcgatccgcc gcggcggcgt gacgctgcgc gatcgccgcg aaggcatgga
     3781 ccagcgtggt catgtccgcc gccaagcggt cgacatcctc gacgcgcgcg taatgggtga
     3841 ggtgcaccgc gcgtggcgca tacgcggcga tacgcgccac cgatgccgcg agctgctgcg
     3901 ggtcgaactg ggtcggcgtc gtcgtcggga agatgaatgc tccgcgcgcg gtgtcaaact
     3961 cgcggtacga caggccgaag gtgtcaccgg tgaacacggt gcgggacgca gggtcggcga
     4021 tcgcctggtg atgcatcgca tgcccgggcg tgaaccagaa ctcgagcacg cggccggcga
     4081 gatcgagttg ctcgccgtcc tgggtcacgt gcacgcgcgc ggcatcgatg ggcacgatct
     4141 cgccatacag ttcgcgatag cgtgactcgc cgtagacctc gatcgaggcg gcgatcagct
     4201 tcgccggatc gatcatgtgc ggcgcaccgc gcggatgcac gacgagcgtg gcgttcggca
     4261 atgcgcgcat gaaggcgccg gcgccgcccg cgtggtcgag atgcacgtgc gtcagcagca
     4321 catagtccac gtccgcgggc tcgcgaccca gcgacgcgag cgcagcgagc acgcgcggca
     4381 cggaatgggc cgtgcccgtg tcgaccagcg ctgcgcggcc gcggtgctcg atcacgtgga
     4441 tcgcggccat ctgcgggcgc acgtagcccg cgtcgatggc cgtgatgtcg tccggcccac
     4501 gcatcgatcg ctatttcgca ggtaccgggt cggccgacag cgccgcgaac agtggcgcga
     4561 ggtcacccac cggctcaccg gccaccgccg agacgtcgaa tgtcttgccg agcgccgcct
     4621 cgttgccgag cgccgcgatg cacacgcgcg cgacatccgc gcgcgcaacc cagctgacct
     4681 cggcgccgac tttctggcgc agcacgatgc gcgtgtgctc gcccggcgcg tccctgaggc
     4741 cgcccggtcg cacgatcgtg tagggcaggc cgctcgcgcg caggtaatcc tcgctcgcgc
     4801 gtttggcagc gaggatcggc cgcatgaacg gcgcgggata ggtctcggca tggtccacac
     4861 cgctcgagga gacgagcacg aactgcgtga cgccggccgc cttcgcggcg tcgacgaggt
     4921 tgatgttgcc ctggcggtcg atttcgtccg gaccgttgcc cggcaccggc ctgagggctc
     4981 cgccaccgat cgtcgagatc acgcgcgtga cgcctttcat ggccggcgcc agggtcgcag
     5041 gatcgcgcac gtcgccgacc acgaactcgg cgctctgcgg caaggtcgcg cgcgccttgg
     5101 cctcatcgcg caccagggcg cgcacccggt aaccctggtc gacgagctgg gtcacgatat
     5161 gcacaccggt gcgcccggtg gccccggcca ccagcacgag gtcggcttcg ctgcttgcgg
     5221 cggcgaaagt cgcgccgccg ccgaggacgg caatcatcag catcgcagcg agttttcgcg
     5281 tcatcgattg tgctcccatg tatgtcacca tatccggatg caacgatcat atacggcctt
     5341 ggcctgcgct gccgtcagcg cactggtcgc gatggcaaca ctgcccgcgc acgcggcgcg
     5401 cgaggagcgc ggcaacctga ccttcgacgg cgtcatccag gcgagtcccg cgctcgtcga
     5461 gcgcatcacg ccgtggctcg cggggcgttc ggccacggtg gcgggctggc tgccggacgg
     5521 tgcgttgctc gtcaccacgc gcttcggcga gatcgcgcag ttgcatcgcg tcgccgcgcc
     5581 gctcggcgcg cgcgaacagc tcaccttcgg cgacgatccg gtgatcgccg ccgcgagcag
     5641 tgcggcgagc ggcgccgcgg gcgtagccta tgtcaccgcc gggcccggcg cggtgccaca
     5701 gctgcgtgcg ctgcgatttg ccgaccgcag caccccgcgc atcgcggcgc cgcgcgtgca
     5761 gccggacgcg ctgacctggt cgccggacgg caggacagtc gccttccagg ccgccagtgc
     5821 cgatggcgtg caccaggaaa tcctcgcgtt cgaaccgggc ggcaacctgc agccgcgcct
     5881 gctcgtcgcg gccggcatca agagttggcg accgctagcg tggtcgcccg acggcggccg
     5941 gctgctgatc caggacgagg ccaactcgca gcacagcgaa ctgtggttcg ctgacgtgaa
     6001 tacgggcggc gttaccgcga tcgcattgcc gacgccgccg cggcgcgtgg tcgcggcgcg
     6061 attcgcgccg gacgggcgca acgtctacct gatcgcgcaa ttcgacgagt tcgcacggct
     6121 ccatcgtctc gaccccgcga ccagccagtt gaccacgatc accgacaacg tgccgtggga
     6181 catcgagagc ttcgcggcct cggccgacgg gcgctacctg gcgttcgtcg cgaatgccgc
     6241 gggcgtgagc cggctgtcgg tgatcgacca gatcgcgcgc atggagctcg cacctcaggg
     6301 gctgcccgag ggccgcatcg ccgatctcgc gttcgacccc gcgggccggc gcctcgcgct
     6361 taccgccgaa ggcgccaccg agccggccga cgcctgggtc tacgagccgg cgagcaacac
     6421 cgtcgcgcgc tggacgcgca gcgagctggg cccgatcgac agggccgcgc tcgtcgcgcc
     6481 cgaattcatc agcttccccg gctgggatcg cgccaacggc agcacgcgtg cgttgcccgc
     6541 gtgggtctac cggccgcgca cgcccgggcc gcaccccgtg ctcgtgcgca tcgcgagcgg
     6601 gccgaacgag cagtaccggc ccggcttcga tgcattcacg cagtttgcgg tcaacgtgct
     6661 cggctacgcg gtcatcgcgc cgaacgtgcg cggtgcaccg ggctacggtc gcagcttcga
     6721 gatgctcggc gagagcgtgc gccgcgagga tgcggtgcgc gacatcggct cactgctggt
     6781 gtggatcggc gtgcagcgcg atctcgaccg ctcgcgcgtc gtgctgatgg gtgaatcgca
     6841 gggcgcgcag gttgcgctgg gctcgctcgc ccagtacggc gatcgcgtcg ccggcggcat
     6901 cagccttgcg ggcatgacga attattcgcg catcccgaat gcgcgcgcgc tgcgccggcc
     6961 gctgctggtc gtgcagggtg cacaggatct cgtcgcaccg gcgttcgagg ccgagcgcct
     7021 cgtcaccacc gtgcgcggca acggcgccga ggtctggtac gtgctcgcga aagaggaggc
     7081 gcacgtgttt cgtcgcaagg cgaaccgcga cgcgtggatg agcgtggcgg cggcgttcct
     7141 ccagcgcctc tccggcagct gatgcctcgg cggaagctgg ctcctcagcg ggcgctgaaa
     7201 cgcgatttgc gcagctcgac cgtctcgagc cccgggccca tcttgccgta gtaagcggcg
     7261 agtgcctcga tgtccttggc gctcagcgtc gcgacgaaac cggccatgat cggattcttg
     7321 cgggcgcccg acttgtagtc ggtgagtgcg cgcttgaggt attcgatgtg ctggccggtc
     7381 aacgtgggat aggcgggcaa cacacccacg ccatcgttgc cgtggcaggc gacacagacc
     7441 gccgcggctt ccggcgcatt ggctgccgcc ggcttgccgg tgggctggac cgcctcgcca
     7501 ccgaggaagg ccgcgatatc ggcgatttcc tgctcggaca gcgacgcggc gtgcgcgtgc
     7561 atcgtggcgt gcgaacgctc ccccgcctgg tagccgcgca gcgccacttc gaggtactcg
     7621 gcgtgctggc cgcgcagctt cggcaccgga taggtgggat agctgttctt gtagttctcg
     7681 accgcgtggc agccgaggca ggtctgggcg agaaccttgc cgcgttcggc attgccctgc
     7741 gcatgcgcga cccccaccga gcccgcgaga aatgtggcga gtgcgaatgc ggttgcggtc
     7801 cggctggcga gcgacatgtc tgacccctgc tgaaatcagc ggcaaatggt acggggtgga
     7861 ctgcccaatt gccactaccg ggttccccgc agggcggccg gatggcacga tcggcgccga
     7921 catgatcgca ttgattcaac gggtcaattt cgccgaagtc acggtcgagg gccggctcgt
     7981 gggtcgcata gggcgcggca ttctcgccct catcggcgtg caggcgctcg acacgccagc
     8041 ctgtggcagc cgcctgctcg agcgcgtgct cgggtatcgg gtattccctg acgaggacgg
     8101 gcgcatgaac cgctcgctcg cggacaccgg gggcggcctg ctgctcgtgc cgcaattcac
     8161 gctggcggcc gatacgcgca agggcgcgcg cgcgggcttc agtactgccg cggctcccga
     8221 agccgcgcag gcgctgttcg ggcagttcgt cgcggaggcg cgacggcgcc atccggaggt
     8281 ggcaaccggc gagttcggcg cacacatgcg ggtggcgctc gaaaacgacg ggccagtcac
     8341 attctggctc gaagcgcacg ccggctgaca cggaactttc ccgtcctcgg cgcctctttt
     8401 ggcctatcat cccgggtttg cgcacgggtg cgcttgtcga ggttcatatg ggtattggga
     8461 tgcgcgagct catcatcatc ctgctggtgg tgctgctggt attcggcgcg aagaaactgc
     8521 gcacgatcgg ttccgatctg ggcgcggcgg tgcgcggctt caagaaatcg gtcagcgagg
     8581 gcgaggccga ggacagcgcc gacgaggtga agcagatcaa gtcggccgag aaggatgccg
     8641 agttccccga agtcgccaag agcgacaccg acagcaagat caaccgcggc gcctgagcgg
     8701 cgcgcgctgc cgccatgttc gaggtcggct tcaccgagct gctgctgatc tttgcacttg
     8761 cactggtcgt gctcggtccc gagaaactgc cgcgagtggc caaccaggtc ggccgctgga
     8821 tcggccgcgc gcgcaccatg gcgcgccagt tccgcgagca actcaagaac gagacgatcc
     8881 tcgag
//
LOCUS       DAOQVY010000100         8863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_8769_length_8863_cov_5.643960, whole genome shotgun sequence.
ACCESSION   DAOQVY010000100 DAOQVY010000000
VERSION     DAOQVY010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8769_length_8863_cov_5.643960"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PKN91_09530"
     CDS             complement(<1..429)
                     /locus_tag="PKN91_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28354.1"
                     /translation="MDWYLAQKQTDQVRPLIDRIHDKGLPAGIAGHNPAVFEWAETAG
                     LPVDFYMCAYYNSAHRDHRAEHVSGMPEWFRDEDREIRTALIQRLSKPVIHYKILAAG
                     RNKPEAAFAYAASHMRPGDAACVGIFDKDDPQMLAKDVAML"
     gene            complement(362..1504)
                     /locus_tag="PKN91_09535"
     CDS             complement(362..1504)
                     /locus_tag="PKN91_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28355.1"
                     /translation="MSRATTDRRAALPSWTAPALLALATVAAYLTSFDGVFQFDDWAV
                     IVDEPRVHSLAAWWQSMPGIRPLLKLSYALNWQGGHGIAGFHAVNLGLHVANACLVLG
                     LLRRLAARLGMAGTLTPLVAALLFALHPVQTEAVTYISGRSSSLAATFALASLWCLEY
                     GRTTTHPALRRLHTLFVSPLLFLLALGVKETTMVVPAAIVLLVSCDARRRVPWREALA
                     AATPHALVLLLAALLAATLPRYWMLLESSLTARDLGVNLLSQMHAVAWLVGQLMMPWR
                     LNADPTLAVVEAWTPPTVALALLYATLLILGCASLRRRPALAPISVLASTLPAPRARR
                     TPEPVRCRRRRTCGRPRHLYPRREDGLVPGPEADRPGPAADRPHPR"
     gene            complement(1485..2294)
                     /locus_tag="PKN91_09540"
     CDS             complement(1485..2294)
                     /locus_tag="PKN91_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNS28356.1"
                     /translation="MRSLAGLALLTCLGVVHGAEAPPITDRYPRAADAYVVAIGATDR
                     WSRAADRSLPPASLTKLMTAVVLLESEWDPDRWVTVSAQAARATGARAGLAEREQITA
                     GDLLAALLVRSANDACIALAEHHAGSVAAFVARMNERSATLALNRTQFTNPCGLDAPG
                     HHSSANDLLRLARHAVKFPSVAALAAARSITISTRKGRQIPLATTNPLLGRLDGAIGL
                     KSGYTAGAGPCVVVHARREDIDVWVVLLNARDRWWTAAALVEDAFHVARHD"
     gene            complement(2321..3319)
                     /locus_tag="PKN91_09545"
     CDS             complement(2321..3319)
                     /locus_tag="PKN91_09545"
                     /inference="COORDINATES: protein motif:HMM:NF023696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HNS28357.1"
                     /translation="MSTLRLAHFATALIAALPALAVLAQEPGETWEVTTEMQVAGMSM
                     PARTQQVCTPRDAPEDVPQGVPTQESCEMYDVRRTGASMSWKLRCAGDPPTSGSGEMH
                     YPDKDNYSGQMQMKVGADEMQMKMRGKRLGTACDAGQVKRQIAAAQAQSARHIEETCR
                     QGVETMAAYLFSGSGTAGIECPASYKEQYCARLSTEAGFDKVAGYGASGQTGGPYEMG
                     PGAAAGVCGLPSAGAGSFEGIRADLCRTALAGESLVFLGRNCVAEGRDLAMRECAGRS
                     YSSPVADKYRDFCTAYARHGALPAGDTGTAAARAPEAPKDSAIKAGKKALRGLIGF"
     gene            complement(3331..3846)
                     /locus_tag="PKN91_09550"
     CDS             complement(3331..3846)
                     /locus_tag="PKN91_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28358.1"
                     /translation="MKRSLIGGTVAVIALAIVWQSLPADTSVPQTAAATPETHAASVD
                     RGRYLVQVAGCNDCHTSRYPELAGKVPEQDWLTGDAVGWSGPWGTTYPINLRLYFADM
                     TAEQWLLIARSVVARPPMPWFNLHVMSDADLLAIYHYVRALGPAGTPAPAYLPPGETA
                     QTPAWHAPTVE"
     gene            complement(3843..4244)
                     /locus_tag="PKN91_09555"
     CDS             complement(3843..4244)
                     /locus_tag="PKN91_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28359.1"
                     /translation="MSDARELDPHHRFVEHYRSRYLPRVRWTAPRRGLEIVGLEAVIH
                     HLLQEAACMGSPEFTPVRRASAPGRVIDEQVVRFVHTGSGLVGAPIARGDLVELERVR
                     IFEFEGDHVKTETHIETWSVLERAPTTGKQP"
     gene            4369..5919
                     /locus_tag="PKN91_09560"
     CDS             4369..5919
                     /locus_tag="PKN91_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HNS28360.1"
                     /translation="MTGAAAPAGRLPRQVPYIIANEACERFSFYGMRNILTPFLVSTL
                     LLFAPEAERAGMAKDVFHTFVIGVYFFPLIGGWIADRYFGKYHTILWLSLVYCAGHAC
                     LALFDDNPRGFYTGLLLIAIGSGGIKPLVASFVGDQFDQSNKARAKVVFDAFYWIINF
                     GSFFASLLMPIFLRHYGPSVAFGIPGLLMLIATLIFWSARHKYVRVPPSGPVPDSFLR
                     VVRAALLIRSPGQRRPGLAVAIVGLVFAAGSLLFVGSIGVVAALCLALVFVMALGGAG
                     AWMQLDRVRGAFAPGAVDGVRAVLRLLIVFALITPFWSLFDQKASTWILQADAMAKPE
                     WFQSAQMQALNPALVMILLPFNNLVLYPALRRFGIDPTPLRRMGFGIAFSGLAWVAAG
                     VIQLWIDGGDAVSVTWQVLPYALLTFGEVLVSATGLEFAYSQAPGSMKGVIMAFWYLG
                     TTVGNLWVLLVNAAVRNDAVIARIAATGLSEAAFLMFFFAAFALVVAAVFGAYARHYP
                     MQDHYRST"
     gene            complement(5804..6409)
                     /locus_tag="PKN91_09565"
     CDS             complement(5804..6409)
                     /locus_tag="PKN91_09565"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HNS28361.1"
                     /translation="MTAFPPIMGVRPRILVLGSLPGVASVRAGQYYAHPRNLFWPIMG
                     ALFDAPPSLPYAQRMRALESAGVCVWDVLAEATRPGSADAAIDPATARAHDIRGLLLR
                     HRGVQLVAFNGAAAARLYRQLVLPTLPPARVASLRHVTLPSTSPAHAALDAAAKLRAW
                     RRALRSSGSDPASGSAARRRRTPPRPPARTPQRKTSGRLPR"
     gene            complement(6439..>8863)
                     /locus_tag="PKN91_09570"
     CDS             complement(6439..>8863)
                     /locus_tag="PKN91_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNS28362.1"
                     /translation="NNPNRLFAALVEAIEPLGAVWDIDPAAFTASASIGGPQLRAAAA
                     ALINALCTVSAPRIVLVLDDLHRLEDGNAFALLESLIERLPEHVALVLGSRVPPALPL
                     ARLRLHGELGELGISDLAFTAGDAAELAARRLAAPLDEVAVQRALARTHGWPAGLALV
                     LARTGAGDRLPTSEARLFEYLAQEVFSGLPPDIQEFLLRTSILAELDAELCAAVTGRS
                     DCREVLQVLYQRNLFVTALDELAPVLRVHDLFRDFLAGRLALRPPEDIAAWHRAAARA
                     ERNPTRAIQHSLAARDWTDAMARIFPLGEQLLAEGAQDSIERWLAEVPASIREESAAA
                     AWLDGMCASSRWDWLRTRTQLTRAVTLLDPDSAERARALAHLADAVNSLGDRPGAARL
                     LDELERNPPDDAGRAQLAALRAWNALPTGDVDQVVHHMRAFVAIVERDPQRLCPYTAG
                     QIHCLFIGIPGIAELFDRYFALAERARGATAAPWQSLALTVGAWAKLWRGRLEEAGNA
                     IARSDELLHRFGDRRNVARGHAQVKCIFLAATGRSEQALAISDAVMGSMDVPDAAGLR
                     LVWRRAYVHGRARLLWMLGREREFLALVPELTAARNEAEWPFLDMAADVVRGQAALIE
                     RDWSRAIDAFELAVAAHPRMRMAMAVADPRIGLARALLGAGRRDAAWQALAPVIDECQ
                     ADDAIGPLLLEPAAHVVAVLELVPDTLRRKPQLAALLARLAEWYAHDEGEELRGPLAE
                     LSERELEVLACVAAGASNKHIARELSLSLHTVKRHIANILAKLDCASRGEAADLYRRC
                     GSSAAGAAR"
BASE COUNT         1279 a         3013 c         3204 g         1367 t
ORIGIN      
        1 cagcatggcc acatccttgg cgagcatctg gggatcgtcc ttgtcgaaga ttcccacgca
       61 ggcagcgtcg ccgggccgca tgtgggaggc agcgtaggcg aaggccgctt cgggcttgtt
      121 tcttcccgcc gcgaggatct tgtagtggat gacgggcttg gagagtcgtt ggatcagggc
      181 ggtcctgatc tccctgtcct cgtcgcggaa ccattccggc atgcctgaca catgctcggc
      241 ccggtggtcg cgatgggccg agttgtagta ggcgcacatg tagaagtcga ccggcagtcc
      301 ggccgtttcg gcccactcga agacagcggg gttatggccg gcgatgcccg cgggaagtcc
      361 cttatcgtgg atgcggtcga tcagcggccg gacctggtcg gtctgcttct gggccaggta
      421 ccagtccatc ttccctccgt ggatatagat ggcgcgggcg cccgcatgtg cggcgccgtc
      481 ggcatcgcac cggttccggc gtccggcgtg cccgcggagc gggcagcgtc gacgccagaa
      541 cggagatggg ggcgagcgcg ggacgccggc gcaatgacgc gcagccgagg atgaggagcg
      601 tcgcgtacag cagcgcgagc gccacggtcg ggggcgtcca tgcctcgacg acggcgagcg
      661 tggggtcggc attgaggcgc cacggcatca tgagctggcc gacgagccag gccacggcat
      721 gcatctggct cagcaggttc acaccgaggt cgcgggccgt gaggctcgac tcgagcagca
      781 tccagtagcg cggcagtgtc gcggcgagca gtgcggcgag caacagcacg agcgcatgcg
      841 gggtggccgc cgccagcgcc tcgcgccagg gcacgcggcg cctcgcatcg cagctcacca
      901 gcagcacgat ggccgccggc accaccatcg tggtctcctt cacgccgagc gccagcagga
      961 acagaagcgg ggagacgaac agcgtgtgca ggcggcgcag cgccgggtgc gtcgtggtgc
     1021 gcccgtactc gaggcaccac aggctcgcga gcgcgaaggt cgcagccagg ctgctcgagc
     1081 gaccgctgat gtaggtgacc gcctccgtct gcacggggtg cagcgcaaag agcagggcgg
     1141 ccacgagagg cgtgagcgtg cccgccatgc cgagccgcgc ggcgagccga cgcagcaggc
     1201 ccagcacgag gcaggcgttc gcgacgtgca ggccgaggtt gacggcgtgg aagccggcga
     1261 tgccatggcc accctgccag ttgagggcat agctcagttt cagcagcggg cggatgccgg
     1321 gcatcgactg ccaccacgcc gcgagcgagt gcacgcgcgg ctcgtcgacg atcacggccc
     1381 agtcgtcgaa ctggaagacg ccgtcgaatg aggtcaggta cgccgccacc gtcgcgagcg
     1441 cgagcagtgc gggcgccgtc caggacggta gcgccgcgcg tcgatcagtc gtggcgcgcg
     1501 acatggaacg catcctccac cagcgccgcg gcagtccacc agcggtcgcg cgcattcagc
     1561 agcacgaccc acacgtcgat gtcctcgcgc cgcgcgtgca cgaccacgca cgggccggcc
     1621 ccggccgtgt agccgctctt gagcccgatc gcgccatcga ggcggcccag cagcggattg
     1681 gtggtcgcga gcggaatctg ccggcccttg cgcgtcgata tcgtgatgct gcgcgcagca
     1741 gcgagtgcgg cgaccgaagg gaacttcacg gcgtggcggg cgaggcgcaa caggtcgttt
     1801 gcggacgagt ggtggccggg cgcgtcgagc ccgcagggat tcgtgaactg cgtgcggttg
     1861 agcgcgagtg tggcgctacg ttcgttcatg cgcgcgacga acgctgcgac gctgcccgcg
     1921 tgatgctcgg cgagcgcgat gcaggcatcg ttggcggagc gcacgagaag cgccgcgagc
     1981 aggtcgccgg ccgtgatctg ttcgcgctcc gcgaggccgg cgcgcgcgcc ggttgcacgc
     2041 gccgcctgtg cactgaccgt gacccagcgg tcaggatccc attcggattc gagcagcacg
     2101 acggccgtca tcagcttggt cagggatgcc ggtgggagcg agcggtccgc cgcgcgcgac
     2161 cagcgatcgg tggcgccgat cgcgaccacg taggcatcgg ccgcgcgcgg atagcggtcg
     2221 gtgatgggcg gcgcctcggc gccgtggacg acgccgaggc aggtgagcag ggcgaggccc
     2281 gccaggctgc gcatcgccca ggcctgcgga cgcagacggt tcagaagccg atcaggccgc
     2341 gcagcgcctt cttgcccgcc ttgatcgccg agtccttcgg cgcttccgga gcccgcgcgg
     2401 cggcggtgcc ggtatcgccg gcaggcagcg caccgtggcg cgcataggcg gtgcagaaat
     2461 cgcggtactt gtcggcgaca ggcgaggagt agctccggcc cgcgcactcg cgcatggcga
     2521 ggtcgcgccc ctcggcgacg caattgcgac cgaggaacac cagcgactcg ccggcgagcg
     2581 ccgtgcggca caggtcagcg cgaatgcctt cgaagctgcc cgcgccggca gacggcaggc
     2641 cgcacactcc ggcggccgcc cccgggccca tctcgtaggg tccgccagtc tggcccgagg
     2701 caccgtatcc ggcgaccttg tcgaagcctg cctcggtcga gaggcgcgcg cagtactgct
     2761 ccttgtagct cgcgggacac tcgatgccgg cggtacccga acccgaaaag aggtaggcgg
     2821 ccatcgtctc gacgccctgg cggcaggtct cctcgatgtg gcgcgccgat tgcgcctgcg
     2881 ccgcggcgat ctgcctcttc acctggcccg catcgcaggc cgtgccgagg cgcttgccgc
     2941 gcattttcat ctgcatctcg tcggcgccga ccttcatctg catctgaccg ctgtagttgt
     3001 ccttgtcggg atagtgcatc tcgcccgatc ccgaagtcgg cggatcgccg gcgcagcgca
     3061 gtttccagct catcgacgcg ccggtgcgcc gcacgtcgta catctcgcag gactcctgcg
     3121 tcggcacgcc ttgcggcacg tcctcgggtg cgtccctcgg agtgcacacc tgctgcgtgc
     3181 gtgccggcat cgacatgccg gccacctgca tttccgtggt gacttcccag gtctcaccgg
     3241 gctcctgcgc gaggacagcg agcgccggca gggccgcgat cagcgcggtt gcaaagtgtg
     3301 caaggcggag cgtcgacatt cggttctcct tcactcgacc gtgggggcat gccaggcggg
     3361 tgtctgcgca gtctctcccg gcggcaggta cgcaggcgcg ggtgtgccgg ccgggcccag
     3421 cgcacggaca taatgataga tggcgagcag gtcggcgtcg ctcatcacat gcaggttgaa
     3481 ccacggcatc ggcgggcgcg caaccacgct gcgggcgatc agcagccatt gctccgccgt
     3541 catgtcggca aagtacaggc gcaggttgat cggataggtc gtaccccacg ggccactcca
     3601 gccgacggcg tcaccggtca gccagtcctg ctcgggcacc ttgccggcga gttcggggta
     3661 acgcgacgta tggcagtcgt tgcagccggc gacctgaacg agatagcggc cacggtccac
     3721 gctggcggcg tgggtttcag gcgttgcggc ggcagtctgc ggcacggacg tgtccgcggg
     3781 cagtgactgc cagacgatgg cgagggcgat gacggcgact gtgccgccga tgagactccg
     3841 tttcatggtt gcttccctgt agttggggcc cgttctagaa cggaccaggt ttcgatatgc
     3901 gtttcggtct tcacgtggtc gccttcgaac tcgaagatgc gcacccgctc gagctcgacg
     3961 aggtcgccgc gggcgatcgg cgcgccgacg agaccgctgc ccgtgtgcac gaagcgcacg
     4021 acctgctcgt cgatcacgcg gccgggggcg ctggcacggc gtaccggggt gaactccggc
     4081 gagcccatgc acgcagcttc ctgcagcagg tggtggatca ccgcctcgag gcccacgatc
     4141 tcgagcccgc ggcgcggcgc cgtccaacgc acgcgcggca ggtaccggct gcggtagtgc
     4201 tccacgaagc ggtggtgggg atcgagttcc ctggcgtccg acatgcagcc attgtcgatc
     4261 ggaggccggc cgcccggcat cgctccgtgg agccattggc ggcgctgcga ggagccattc
     4321 ggcgtcttgc gggttgacgg cggccttccg acacggcagc ttggccacat gaccggcgca
     4381 gccgcaccgg ccgggcgcct gccgcgccag gtgccgtaca tcattgcgaa cgaggcgtgc
     4441 gagcgcttca gcttctacgg gatgcggaac atcctcaccc cgttcctcgt ctcgaccctg
     4501 ctgctgtttg cacccgaggc cgaacgcgcg ggcatggcga aggacgtgtt ccacaccttc
     4561 gtcatcggcg tgtacttctt tccgctgatc ggcggctgga tcgcggaccg ctacttcggc
     4621 aagtaccaca cgatactgtg gctgtcgctc gtgtactgcg ccggccacgc ctgcctggcg
     4681 ctgttcgacg acaacccccg cggcttctac accggcctgc tgctcatcgc gatcggctcg
     4741 ggcggcatca agccgctggt cgcgtcgttc gtcggcgacc agttcgacca gtcgaacaag
     4801 gcgcgcgcga aggtcgtgtt cgacgcgttc tactggatca tcaatttcgg ttcgttcttc
     4861 gcatcgctgc tgatgccgat cttcctgcgc cactacggcc cgagcgtcgc cttcggcatt
     4921 cccggcctgc tgatgctgat cgccacgctg atcttctggt cggcgcgcca caagtacgtg
     4981 cgggtgccac cgtcaggtcc ggtcccggac tcgttcctgc gcgtggtacg tgcagcgctg
     5041 ctcatacgct cccccggcca gagacggccc ggcctcgccg tagcgatcgt cggcctcgtg
     5101 ttcgcggcgg gctcgctgct gttcgtgggc tcgatcggcg tggtcgctgc gttgtgcctg
     5161 gcgctggtgt tcgtcatggc gctcggcggc gccggcgcgt ggatgcaact cgaccgggtc
     5221 cgcggtgcgt tcgcgcctgg ggcagtcgac ggcgtgcgtg ccgtgctgcg cctcctgatc
     5281 gtgttcgcgc tcatcacgcc gttctggtcg ctgttcgacc agaaggcatc gacctggatc
     5341 ctgcaggccg acgccatggc caagcccgaa tggttccagt cggcgcagat gcaggccctg
     5401 aatcccgcac tggtgatgat tctgctgccg ttcaacaacc tcgtgctgta tccggcgctg
     5461 cgccgcttcg gaatcgaccc gacgccgctg cgccgcatgg gtttcggcat cgcgttctcc
     5521 gggctggcgt gggtcgcggc cggcgtgatc cagctgtgga tcgacggcgg cgatgccgtg
     5581 tcggtgacct ggcaggtgct gccctacgcg ctgctgacct tcggcgaggt gctggtgtcg
     5641 gccaccggcc tcgagtttgc ctacagccag gcgcctgggt cgatgaaggg cgtcatcatg
     5701 gcgttctggt acctggggac caccgtcggc aacctgtggg tgctgctcgt caatgcggcg
     5761 gtccgcaacg acgcggtcat tgcgcgcatc gccgccaccg ggctcagcga ggcagccttc
     5821 ctgatgtttt tctttgcggc gttcgcgctg gtggtcgcgg cggtgttcgg cgcctacgcg
     5881 cggcactacc cgatgcagga tcactaccgc tcgacctgag cgcgcgccgc cacgcgcgca
     5941 gtttcgcggc cgcgtcgagc gccgcatgcg ccggactggt cgagggcagc gtcacgtgac
     6001 gcagcgaggc gacccgggcc ggcggcaggg tcggcaacac gagctgccgg tagagccgcg
     6061 ctgcggcggc gccgttgaag gcgacgagct gcactccgcg atggcgcagc agcagcccgc
     6121 ggatgtcatg cgcccgcgcc gtggcggggt cgatcgcggc atccgcgctg cccgggcgcg
     6181 tcgcctcggc cagcacgtcc cacacgcaca ccccggcgga ttcgagcgcg cgcatgcgct
     6241 gcgcgtaggg cagggacggt ggtgcgtcga acaacgcgcc catgatcggc cagaagagat
     6301 tccgcggatg ggcgtagtac tggccggcgc gcaccgaggc gactcccggc aggctgccga
     6361 gcaccaggat gcgcggccga acgcccatga tcggcggaaa cgcggtcacg tgcgtcatgc
     6421 ccgcgggtcg gccggcgctc agcgcgccgc gccggccgcg gaggagccgc agcgccggta
     6481 caggtccgcg gcctcgccgc gcgaggcgca atcgagcttc gcgaggatat tcgcgatgtg
     6541 ccgcttcacg gtatgcagcg acagcgacag ctcgcgcgcg atgtgcttgt tgctggcacc
     6601 cgccgcaaca catgccagta cctcgagctc gcgctcgctc agttcggcga ggggtccgcg
     6661 cagctcctcg ccttcgtcgt gcgcatacca ttcggcgagc cgcgcgagca gcgcggcgag
     6721 ctgcggcttg cgacgcagcg tgtccggcac gagttcgagc acggcgacga catgtgccgc
     6781 gggctcgagc agcagcggtc cgatcgcatc gtcggcctgg cactcgtcga tcaccggtgc
     6841 cagcgcctgc cacgccgcgt cgcgccggcc ggcgccgagc agggcgcgcg cgaggccgat
     6901 gcgcgggtcc gccaccgcca tcgccatgcg catgcgcggg tgagcagcca cggcgagctc
     6961 gaaggcatcg atcgcgcgcg accagtcccg ctcgatcagc gccgcctggc cacgtacgac
     7021 gtcggcggcc atgtcgagga agggccactc cgcctcgttg cgggcggcgg tcagctccgg
     7081 gaccagcgcc aggaactcgc gctcgcgacc cagcatccac agcagccgcg ccctgccgtg
     7141 cacataggcg cgccgccaca cgagcctcag gccggcggcg tccggcacat ccatgctccc
     7201 catcaccgca tcgctgatgg cgagtgcctg ctcactgcgg ccggtggcgg cgaggaagat
     7261 gcacttgacc tgtgcatgac cgcgcgcgac gttgcgccgg tcgccgaagc ggtgcagcag
     7321 ttcatcgctg cgcgcgatcg cgttgccggc ttcctccagc cgcccgcgcc acagcttcgc
     7381 ccacgcgccg accgtgagcg ccagcgactg ccagggcgcg gcagtcgcgc cgcgcgcgcg
     7441 ctcggcgagc gcgaagtagc gatcgaagag ctccgcgatg cccgggatgc cgatgaagag
     7501 gcaatgtatc tggccggcgg tgtacgggca gagccgctgc gggtcgcgct cgactatcgc
     7561 gacgaaagcg cgcatgtggt gtaccacctg atcgacgtca cctgtcggca gcgcgttcca
     7621 ggcgcgcagt gcggcgagct gtgcgcgccc ggcgtcgtcg ggcggattcc gttcgagttc
     7681 gtcgagcagc cgcgcagcgc ctggccggtc gccgagcgag ttgaccgcgt cggcaaggtg
     7741 agcgagcgcg cgcgcccgtt cggctgagtc cgggtcgagc agtgtgaccg cccgcgtcag
     7801 ctgggtccgc gtgcgcagcc agtcccagcg ggaagaggcg cacatgccgt cgagccacgc
     7861 cgcggcggca ctctcctcgc ggatcgatgc aggcacttcc gcgagccagc gttcgatgct
     7921 gtcctgcgcg ccctcggcga gcagctgctc gccgagcggg aagatgcgtg ccatggcgtc
     7981 ggtccagtcg cgcgccgcga ggctgtgctg gatcgcgcgg gtcgggttgc gctcggcgcg
     8041 tgcggcggcc cgatgccagg cggcgatgtc ttcgggcggg cgcagcgcga gccgcccggc
     8101 gaggaagtcg cggaacaggt cgtgcacgcg cagcaccggg gcgagctcgt cgagcgcggt
     8161 aacgaacagg ttgcgctggt agagcacctg cagtacttcg cggcaatcgc tgcgtcctgt
     8221 gaccgccgcg cacagttccg cgtcgagttc ggcgagtatc gaggtgcgca gcaggaattc
     8281 ctggatgtcg ggtggcaggc cggaaaacac ctcctgcgcg aggtattcga acagccgggc
     8341 ctccgaggtg ggcaggcggt cgcctgcgcc ggtgcgtgcg agcacgaggg cgagcccggc
     8401 aggccagccg tgcgtgcggg ccagggcacg ctgcacggcg acctcgtcga gcggggcggc
     8461 gagtcggcga gcggcgagct cggccgcgtc gccggccgtg aaggcgagat cactgatgcc
     8521 gagttcgccg agttcgccgt gcaggcgcag gcgggcaagc ggcagggcgg gcggcacgcg
     8581 cgagccgagc actagcgcca cgtgctcggg caggcgttcg atcagcgact cgagcagcgc
     8641 gaacgcattg ccgtcctcga gtcgatgcag gtcgtccagc accagcacga tgcgcggggc
     8701 actcaccgtg cacagtgcgt tgatgagcgc cgccgccgcg gcgcgcagtt gcggcccgcc
     8761 gatcgacgca ctggcagtga acgcggcggg atcgatgtcc cagaccgcgc cgagcggttc
     8821 gatcgcttcg acgagcgccg cgaacaggcg gttcggattg ttg
//
LOCUS       DAOQVY010000101         8730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_8944_length_8730_cov_4.903170, whole genome shotgun sequence.
ACCESSION   DAOQVY010000101 DAOQVY010000000
VERSION     DAOQVY010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8730
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8944_length_8730_cov_4.903170"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PKN91_09575"
     CDS             complement(<1..260)
                     /locus_tag="PKN91_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNS28363.1"
                     /translation="MSGEFDARAFVATLPRRPGVYRMYGADGELLYVGKAQRLRDRVG
                     SYFGPRALAPKVAALVGQVARVEVTITRSDTEALLLEYNLIK"
     gene            complement(257..916)
                     /locus_tag="PKN91_09580"
     CDS             complement(257..916)
                     /locus_tag="PKN91_09580"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNS28364.1"
                     /translation="MPDAAACFDAQCSRCPRLVAYRNESRYRYPEYHSAPVPAFGDAD
                     PRLLIVGLAPGLHGANRTGRPFTGDHAGLLLYETLHALGLGSQPQSVAADDPLQLFEV
                     RITNAVKCVPPENKPTPAETRNCLGYLDAELAALARVRAVLALGRIAHDAVLAALGIK
                     RAAFTFAHGAEHALPGGRWLIDSFHCSRYNTQTRRLTAAMFHTVLARAATLSGLSVPG
                     P"
     gene            complement(1060..2253)
                     /gene="icd"
                     /locus_tag="PKN91_09585"
                     /pseudo
     CDS             complement(1060..2253)
                     /gene="icd"
                     /locus_tag="PKN91_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
     gene            complement(2288..3565)
                     /gene="aceA"
                     /locus_tag="PKN91_09590"
     CDS             complement(2288..3565)
                     /gene="aceA"
                     /locus_tag="PKN91_09590"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020394982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HNS28365.1"
                     /translation="MRSATDLTRDWQDNPRWRGITRGYTAADVVRLRGTVHVEHSIAR
                     LTSEKLWKYVTTKPFVNALGALTGNQAMQQVKAGLDAIYLSGWQVAGDANLAGQMYPD
                     QSLYPADSVPAVVKRINSTLRRADELHHAEGDDSIDWFKPIVADAEAGFGGVLNAFEL
                     MKQMIEAGAAGVHFEDQLSSAKKCGHMGGKVLVPTQEAINKLVAARLAADVCDVPTVL
                     IARTDAESANLLTSDIDERDAPFIESRERTSEGFFRVKAGLGQAVARGLAYAPYADMI
                     WCETGHPDLEEAKTFAEGIHRQFPGKLLAYNCSPSFNWKKKLDDATIGKFQRELGAMG
                     YKFQFITLAGFHALNYSMFQLAKGYRESQMTAYVRLQQEEFAAESLGYTATKHQREVG
                     AGYFDEVTQAVTGGQSSVTAMTGSTEEQQFRSA"
     gene            complement(3584..5179)
                     /gene="aceB"
                     /locus_tag="PKN91_09595"
     CDS             complement(3584..5179)
                     /gene="aceB"
                     /locus_tag="PKN91_09595"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HNS28366.1"
                     /translation="MAKGSAAALEIKASVSAAQGEILTPEALTFVAALADRFAPRVAT
                     LLEAREARQRRIDAGELPDFLPETRAIREGDWRVAPIPADLQDRRVEITGPTERKMII
                     NALNSGARMFMADCEDSLSPGWDNVIDGQTNLRDAVRRTISFVSPEGKQYRLNAETAV
                     LIVRPRGWHLYEKHLLLNGKQVPGALVDFGLYLFHNARELLARGTGPYFYLPKLESHH
                     EARLWAEVFAFAERELGLAHGTIRCTVLIETILAAFEMDEILHELRDYIVALNCGRWD
                     YIFSFIKKFSRRPDFLLPDRQQVTMTTHFLRSYSLLCIKTCHRRGAFAMGGMAAQIPI
                     KNDPQANEQALAKVRADKEREAGDGDDGTWVAHPALVPVAMEVFDRLMPGPNQVARLR
                     EDVQVSARDLLAVPTGTITFEGLRNNVSVSLQYLAAWLSGNGCVPIHNLMEDAATAEI
                     ARAQIWQWIRHPGGVLDDGRRVTLAMFRELLAAEKARLAATLGAAQYAGGNFDAAAEL
                     LDAITAADEFESFLTLSAYRRLD"
     gene            5286..6770
                     /locus_tag="PKN91_09600"
     CDS             5286..6770
                     /locus_tag="PKN91_09600"
                     /inference="COORDINATES: protein motif:HMM:NF023689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3612 domain-containing protein"
                     /protein_id="HNS28367.1"
                     /translation="MAATTVRQGALLGSKLRALRKRNGLTLDELSARCVHLDPDIAPS
                     VSYLSMLETGKRTPSPAVLALVASVFGRPAEWFLDGNFEGTPADERNESAGNLDATAF
                     EPSFLFSPAMLRHALPELLLQTGTSGHAFAQLLIRVWQETQQNDFPDIERAAEAAGGR
                     EMPLSLDALVAICERHGLEIRWVDDDRRRLNRGLARARFEAPGTIFASRRLRVREERL
                     KYELAFFLGHRILHNGDGAISAAYVDRTDTEAAAPAAAGLGPRDTLHAWREFECSFFA
                     GALLCPRAPFRQLLVRESHRIAVHRKLGVGPAVFMRRMTAVSPYRHWHFFDGYPPGYL
                     RTVYRGNGIALPWGNLSVVPDPCPHWAVFRLLRAHAAGEAAPTRPTSQISAMDDGGQL
                     RLFCCHSLLTRDAADATRVLSVGIDLAPALAAQGIDAAGVISQVWDACQRGGGAAAIP
                     EDAATGLRTVAHVLKIGWIADALDTSAQIICPRSAACPRGKPCS"
     gene            complement(6817..8175)
                     /locus_tag="PKN91_09605"
     CDS             complement(6817..8175)
                     /locus_tag="PKN91_09605"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HNS28368.1"
                     /translation="MRTTDLTTWHPASWQAKPAQQQPRYPDAAALDRAVAELSRLPPI
                     VVSWEVDALRERLAAAQRGEAFLLQGGDCAETFEGCESDKIAKQLKILLQMSLVLLHG
                     LKMPVIRVGRMAGQYAKPRSADTETRDGVTLPSYRGDLVNHPEFTAEARTPDPTLLLR
                     GYERAALTLNFTRALVAGGFADLHHPEYWDLGFVHHSPLKDAFRRIATSIADSLDFLE
                     GLSGRRVHEATHAGFFASHEGLVLHYEQAQTRFIPRQNRWYDLSTHMPWIGMRTAQLD
                     GAHIEFFRGIANPIGVKIGAAMDDEWLQGLVATLNPQNVPGRLALIHRFGVKEIERTL
                     PRMINAVRQTGHSVLWICDPMHGNTETSTGGLKTRRFDNILKEIDLAFRIHRDLGSVL
                     GGVHIELTGEDVTECTGGARGLSDADLQRAYRSTVDPRLNYEQSLELALLIAERAHAR
                     RG"
     gene            8299..>8730
                     /locus_tag="PKN91_09610"
     CDS             8299..>8730
                     /locus_tag="PKN91_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNS28369.1"
                     /translation="MNDKDIKALLTRLGLTGATLESGGGAQIEVTNPATGALLARARG
                     ASDAEYERVMARAVDAAAAWRAVPAPKRGEAVRLLGEALRRHKDDLGALVTLENGKIR
                     AEGLGEVQEMIDIADLAVGQSRMLYGLTMHSERPQHRMYEQY"
BASE COUNT         1340 a         2896 c         2978 g         1516 t
ORIGIN      
        1 tccttgatca ggttgtactc gagcagcagc gcctcggtat ccgaccgggt gatcgtgacc
       61 tcgacgcggg ccacctggcc gacgagcgcg gcgaccttcg gcgccagggc gcgcgggccg
      121 aagtagctcc cgacccgatc gcgcagccgc tgcgccttgc cgacatacag aagctcgccg
      181 tcggcgccgt acatgcgata gacacccggc cgccgcggca gcgttgcgac gaaagcgcgg
      241 gcatcgaact ccccgctcat ggccccggca ctgacagtcc ggacagcgtg gctgcgcgcg
      301 cgagcacggt atggaacatc gctgcggtca ggcgccgggt ctgggtgttg tagcggctgc
      361 agtggaagga gtcgatgagc cagcgaccgc ccggcagcgc atgctcggca ccgtgcgcga
      421 atgtgaatgc cgcgcgtttg atgccgagcg cggcgagcac cgcgtcgtga gcgatgcgcc
      481 cgagcgcgag cacggcgcgc accctggcga gtgccgcaag ttctgcgtcc aggtacccga
      541 ggcagttgcg cgtctcggcg ggtgtcggct tgttctcggg cggcacgcac ttcacggcgt
      601 tggtgatgcg cacctcgaag agctgcagcg gatcgtccgc cgccaccgac tgcggctgtg
      661 aaccgaggcc gagcgcgtgc agcgtctcgt acagcagcag gccggcatgg tcaccggtga
      721 acggccggcc ggtgcgattc gccccgtgca gaccgggggc gagaccgacg atcagcagcc
      781 gcggatcggc gtcaccgaaa gccggcaccg gcgcactgtg gtactcgggg tagcggtagc
      841 gcgattcgtt gcgatacgcg acgagtcgcg gacagcggct gcactgggcg tcgaagcacg
      901 cggcggcgtc aggcacggcc gcgctcagtc gtccatgtga cggatgatcg cctgcgcgaa
      961 tccggagcag ctcatcagcc tggcgccggg aatcagccgc tggatgtcct cctccacatt
     1021 gcggcgcgcg gcgatgtcgc gcttcggtgc gcggatcgcc tcgcggaggc gagcgaggtc
     1081 gtaggtgagt tccttggcgg cgatggtgtt cgcgaccccc ttgacgatga ggtcggccgc
     1141 ctcgttccac ttgaggtagc gcagcatcat ttccgccgag aggatcagcg agcccgggtt
     1201 gacgcgatcc ttgccggcaa agcccggggc cgtgccgtgt gtcgcttcga agaccgcgag
     1261 gccgtcaccg atattggcgc ccggagcgat gccgatgccg ccgacctgcg cggcgagcgc
     1321 atccgaaatg tagtcgccgt tcaggttgag cgtggcgatg acgtcatatt cctcgggtcg
     1381 cagcagcacc tgctgcagga agttgtcggc gataacgtcc ttcaccacga tgtcggtgcc
     1441 ggtgagcgga ttcctgaagc tgagccacgg accgtcgccg atctccgtgg caccgaattc
     1501 gtcctttgcc aactggtagc cccagttgcg gaacgcaccc tcggtgtact tcatgatgtt
     1561 gcccttgtgc accagggtca ccgagacgcg gtcgtgttcc accgcgtact ggatcgcctt
     1621 gcgcaccagg cgctggctgc cttccttcga taccggcttg atgccgatgc cagagctcgc
     1681 cgggaagcgg atgccggtcg cgcgcagctc ggtctgcaga tagtggatca gcttctgcac
     1741 ttcctgcgag cccgccgggt attcgatgcc ggcgtagatg tcctcggtgt tctcgcggaa
     1801 gaccaccatg tcggtgaggc tcgcgtcctg catcgggctc gcgacacccg ggaaatagcg
     1861 gatcgggcgc acgcaggcgt agaggtcgag atgctggcgc atcgccacgt tgagcgaacg
     1921 gatgccgccg cccaccgggg tcgccagcgg tcccttgatc gacacgacgt attcgcgcag
     1981 cgcgtgcagg gtctcgtcgg gaaaccagct gccgtagatg cggttggcct tctcgcccgc
     2041 gtagatttcc atccacgcga tgcggcgccg gccgcggtag gccttctcta ccgcggcctc
     2101 caccaccgcc agcatcgcgg gcgtgacgtc gatgccgatg ccgtcgccct cgatgtacgc
     2161 gatgatcggg cgctccggca cattgatcgt cgcgtcggga ttgacggtga ttcgttcacc
     2221 gccggctgga accttgatgt ggtcgtactt catcaaggaa ttttaccagc acggcggcgt
     2281 attcgcgtca ggcgctcctg aactgctgct cctcggtcga tccggtcatc gcagtgaccg
     2341 aggactgccc gccggtgacc gcctgggtga cctcgtcgaa gtagccggcg ccgacctcgc
     2401 gctggtgctt ggtcgccgtg tagccgagcg actccgcggc gaactcctcc tgctgcagcc
     2461 gtacgtaggc cgtcatctgg ctttcgcgat agcccttggc cagctggaac atcgagtagt
     2521 tgagcgcatg gaagcccgcg agcgtgatga actggaactt gtaacccatg gcgccgagct
     2581 cgcgctggaa tttgccgatc gtcgcgtcgt cgagcttctt cttccagttg aaggacggtg
     2641 agcagttgta cgcgagcagc ttgcccggaa actgccgatg gatgccctcg gcgaaggtct
     2701 tcgcttcctc gaggtcgggg tggcccgtct cgcaccagat catgtccgca tagggcgcgt
     2761 acgcgaggcc gcgtgccacc gcctgcccga ggccggcctt gacgcggaag aagccttcgc
     2821 tggtgcgctc gcgcgattca atgaacggtg catcgcgctc gtcgatatcg gaagtcagca
     2881 ggttcgccga ctcggcatcg gtgcgcgcga tcagtacggt cggcacgtcg cagacgtcgg
     2941 ccgcgaggcg cgccgcaacc agcttgttga tcgcctcctg cgtcggcacg agcaccttgc
     3001 cgcccatgtg gccgcacttc tttgccgagg acagctggtc ctcgaagtgc acacccgcgg
     3061 cacccgcctc gatcatctgc ttcatcagct cgaaggcgtt cagcacgccg ccgaacccgg
     3121 cttcggcgtc ggcgacgatc ggcttgaacc agtcgatcga gtcgtcgccc tcggcgtggt
     3181 gcagttcatc ggcgcgcctg agcgtgctgt tgatgcgctt gacgactgcc ggcaccgaat
     3241 cggccggata cagcgactgg tccggataca tctgtccggc caggttcgcg tcgcccgcca
     3301 cctgccagcc cgacaggtag atcgcatcaa ggccggcctt gacctgctgc atggcctggt
     3361 tgccggtcag cgcgccgagt gcgttcacga agggcttcgt ggtgacgtac ttccagagtt
     3421 tttcgctcgt aagtcgtgca atcgagtgtt cgacgtggac ggtgccgcgc aggcgcacca
     3481 cgtccgcggc ggtatagccg cgcgtgatac cccgccaacg ggggttgtcc tgccagtcgc
     3541 gggtcaggtc ggtggcgcta cgcatcgcag aatctccgtg gtgtcagtcg agccgccggt
     3601 aggcggaaag ggtgaggaag ctttcgaatt cgtccgctgc ggtgatggca tcgagcagtt
     3661 cggccgcggc atcgaagttg ccgccggcat actgcgcggc gccgagcgtg gccgcgaggc
     3721 gtgccttttc ggccgccagc agttcgcgga acatcgcgag cgtgacgcgc cgaccatcgt
     3781 cgagcacgcc gcccggatgg cggatccact gccagatctg cgcgcgcgcg atctcggcgg
     3841 tcgccgcgtc ctccatcagg ttgtggatcg gcacgcagcc attgccggac agccaggccg
     3901 ccaggtactg cagcgacacg ctgacgttgt tgcgcagccc ctcgaacgtg atcgtgcccg
     3961 tcggcacggc gagcaggtcg cgggcgctga cctgcacgtc ctcgcgcagg cgcgccacct
     4021 ggttcgggcc cggcatcagc cgatcgaaca cctccattgc cacgggcacg agcgcgggat
     4081 gcgcgaccca ggtgccgtcg tcgccgtcgc ccgcctcccg ctccttgtcg gcgcgcacct
     4141 tcgcgagcgc ctgctcgttc gcctgcgggt cgttcttgat cgggatctgc gcggccatgc
     4201 cgcccatggc gaacgcgccg cggcggtggc aggtcttgat gcacagcagg gagtacgagc
     4261 gcaggaagtg cgtcgtcatc gtcacctgct gccggtcggg cagcaggaaa tcggggcggc
     4321 gcgagaattt cttgatgaag ctgaagatgt agtcccatcg tccgcaattg agcgcgacga
     4381 tgtagtcgcg cagctcgtgc aggatctcgt ccatctcgaa ggccgcgagg atggtctcga
     4441 tcagcaccgt gcagcggatc gtgccgtgcg cgagcccgag ttcgcgctcg gcgaaggcga
     4501 acacctccgc ccacagccgc gcctcgtggt ggctctcgag cttcggcagg tagaaatacg
     4561 gccccgtccc gcgcgccagc agctcgcgcg cgttgtggaa caggtacagg ccgaagtcga
     4621 ccaatgcgcc tggcacctgt ttgccattga gcagcaggtg cttttcgtag agatgccagc
     4681 cgcgcggccg cacgatcagc acggctgtct cggcattgag ccgatactgc ttgccctcgg
     4741 gactcacgaa actgatggtg cggcggaccg cgtcgcgcag attcgtctgg ccgtcgatca
     4801 cgttgtccca gccgggcgag agcgagtctt cgcagtcggc catgaacatg cgcgcgcccg
     4861 aattgagcgc gttgatgatc atcttgcgtt cggtgggccc ggtaatctcg acgcgccggt
     4921 cctgcagatc ggcgggaatc ggcgccacgc gccagtcgcc ttcgcggatt gcgcgcgtct
     4981 ccggcaggaa atccggcagt tcaccggcat cgatgcgccg ctggcgcgct tcgcgtgcct
     5041 cgagcagggt cgccacccgg ggcgcgaagc ggtccgcaag agccgcgacg aaggtcaggg
     5101 cctcgggcgt caggatctcg ccctgcgccg cgctgaccga tgccttgatc tcgagcgctg
     5161 ctgcgctgcc tttcgccatg tactgctccc cggtgggctg gtgcgattgc gcgaccctgg
     5221 caggcagttt atggtatctg ggagtgattt acacaataga aatttgacac agtaaacttt
     5281 acattatggc tgcaacgacc gtccgccagg gcgccctgct cggttcgaag ctgcgtgccc
     5341 tgcgcaagcg caatggactc acgctcgatg agctttcggc gcgctgcgtg catctcgatc
     5401 cggacatcgc accctcggtg tcctatctca gcatgctcga gaccggcaaa cgcacgccct
     5461 ccccggccgt gctcgcgctg gtggccagtg tgttcggcag gcctgccgag tggtttctcg
     5521 acggcaactt cgaaggcacg ccggccgacg agcgcaacga gagcgcgggt aacctcgatg
     5581 ccacggcatt cgaaccctcc ttcctgttct cgccggcgat gttgcgccat gcgctgccgg
     5641 agctgctgct gcaaaccggc acttcgggcc acgcctttgc acaactgttg atccgggtct
     5701 ggcaggagac ccagcagaat gactttccgg acatcgagcg cgcggcggag gctgccggag
     5761 ggcgcgagat gccgctttcg ctcgatgcgc tggtcgcgat ctgcgagcgc cacggcctcg
     5821 agatccgctg ggtcgacgat gaccggcgcc gcttgaaccg cgggctcgcg cgagcacgct
     5881 tcgaggcccc gggtacgatc ttcgcgagcc gccgcctgcg cgtgcgcgag gagcggctga
     5941 aatacgagct cgcgttcttc ctcggccaca ggatcctgca caacggcgat ggcgcgatct
     6001 cggcggccta tgtggatcgc accgacacgg aagcggctgc gcccgccgcg gcggggctcg
     6061 gcccgcgcga cacgctgcat gcctggcggg aattcgagtg cagcttcttt gccggcgcgc
     6121 tgctgtgtcc gcgcgcaccg tttcgacagc tgctggttcg cgagtcgcat cgcatcgcag
     6181 tgcatcgcaa gctcggcgtg ggtcccgctg tcttcatgcg acgcatgacc gcagtctccc
     6241 cttaccgcca ctggcacttc ttcgatggct atccgcccgg ctatctgcgc accgtgtacc
     6301 gcggcaacgg catcgcgctg ccctggggca acctctcggt cgtgcccgac ccctgtccgc
     6361 attgggcggt attccgcctg ctgcgcgcac atgccgccgg cgaggcggcg ccgacgcggc
     6421 ccacctcgca gatctccgcc atggacgacg gcggccagct gcgactcttc tgctgccact
     6481 cgctgttgac acgcgatgcg gccgacgcca cgcgcgtgct gtcggtgggc atcgatctcg
     6541 ccccggcgct cgccgcgcag gggatcgatg ctgcaggcgt catctcgcag gtctgggatg
     6601 cgtgtcagcg cgggggcggc gctgcggcaa tcccggagga tgcggccacg ggtctgcgca
     6661 cggtggcgca tgtgctcaag atcggctgga tagcagatgc gctcgacaca tcggcacaaa
     6721 tcatctgccc gcgcagtgcc gcgtgcccgc gcggcaaacc ctgcagctga gccgctggag
     6781 ccttgctgcg cccgaggccc gggtgctggt gcctgactag ccgcgtcgcg cgtgcgcgcg
     6841 ctcggcgatc agcagtgcga gctcgaggct ctgctcgtag ttgagccgcg gatccaccgt
     6901 ggagcgatag gcgcgctgca ggtcggcgtc cgacaggccg cgcgcgccgc cggtgcactc
     6961 ggtcacgtcc tcaccggtga gttcgatgtg cactccgccc agcaccgaac ccaggtcgcg
     7021 gtggatgcgg aacgccaggt cgatttcctt caggatgttg tcgaagcggc gcgtcttcag
     7081 gccgccggtg ctcgtttcgg tattgccgtg catcggatcg cagatccaca gcacgctgtg
     7141 gccggtctgg cgcacggcat tgatcatgcg cggcagcgtg cgctcgattt ccttcacacc
     7201 gaagcgatgg atcagcgcga ggcggcccgg cacgttctgc gggttcaagg tggcaaccag
     7261 gccctgcagc cactcgtcgt ccatcgccgc gccgatcttc acgccgatcg gattggcgat
     7321 gccgcggaag aactcgatgt gcgcgccgtc gagctgggcc gtgcgcatgc cgatccacgg
     7381 catgtgggtc gaaaggtcgt accagcgatt ctggcgcggg atgaagcggg tctgcgcctg
     7441 ctcgtagtgc agcaccaggc cttcatggct cgcgaagaac cccgcgtggg tggcctcgtg
     7501 cacgcgtcgc ccgctcaggc cctcgagaaa gtcgagcgaa tcggcgatcg aggttgcgat
     7561 ccgccggaac gcgtccttca gtggcgagtg gtgcacgaaa ccgagatccc agtactcggg
     7621 gtggtgcagg tcggcgaagc cgcccgcgac cagcgcgcgc gtgaagttga gggtgagcgc
     7681 cgcgcgctcg tagccgcgca gcagcaaggt cggatcgggc gtgcgcgcct cggcggtgaa
     7741 ctcggggtga ttgacgagat cgccgcggta gctcggcagc gtgacaccgt cgcgcgtctc
     7801 ggtgtcggcc gagcgcggct tggcatactg ccccgccatg cgcccgacgc ggatcaccgg
     7861 cattttcagg ccgtgcagga gcacgaggct catctgcagc aggatcttca gctgcttggc
     7921 gatcttgtcg gactcgcagc cctcgaaggt ttcggcgcag tcgccgccct ggagcaggaa
     7981 ggcctcgccg cgctgcgcgg cagccaggcg ttcccgcagc gcatcgacct cccaggagac
     8041 gacgatcggc ggcaggcgcg acaattccgc gaccgcgcga tcgagcgccg ctgcgtccgg
     8101 atagcgcggc tgttgctgcg cgggtttcgc ttgccagctc gccggatgcc aggtcgtgag
     8161 atcggttgta cgcatccgtc caatattaca cctgaaggcg caactgtggc ggtctggcag
     8221 atggacgggc agaatgcgcg gcctccagta aggaaagccg ccggaatgcc gccggcgcaa
     8281 gccgggagct gcccgccaat gaacgacaag gatatcaagg ccctgcttac gcgactcggg
     8341 ctcaccggcg cgacactcga atccggcggc ggcgcgcaga tcgaggtcac gaatccggct
     8401 acgggcgcac tgctcgcgcg cgcgcgtggg gcgtccgatg ccgagtacga gcgtgtcatg
     8461 gcgcgtgcgg tcgatgccgc cgcggcctgg cgcgccgtgc ctgcaccgaa gcgcggcgag
     8521 gccgtgcgcc tgctcggcga ggcgctgcgc aggcacaaag acgacctggg cgcgctcgtg
     8581 acgctcgaga acggcaagat ccgcgccgag ggcctcggcg aagtgcagga aatgatcgac
     8641 atcgccgacc tcgcggtcgg ccagtcgcgc atgctgtacg gcctgacgat gcactccgag
     8701 cggccgcagc accgcatgta cgagcagtac
//
LOCUS       DAOQVY010000102         8729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_8947_length_8729_cov_5.375951, whole genome shotgun sequence.
ACCESSION   DAOQVY010000102 DAOQVY010000000
VERSION     DAOQVY010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8947_length_8729_cov_5.375951"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2692
                     /locus_tag="PKN91_09615"
     CDS             <1..2692
                     /locus_tag="PKN91_09615"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNS28370.1"
                     /translation="AALRDDVHALGGLVGEILREQGGDALFEIVERDRVLAIARRAGE
                     ADAAAELEVRVRGRPPAEARDLVRAFSAWFQAVNLAEKVHRVRRRKEYFLADSERPQP
                     GGVEDALATLHAGGASLEEVLALLAKLRIEPVFTAHPTESTRRTILRKQQRVAEMLLD
                     RLDPTLTPQETRALWGRVRTEMTTAWQTEDHPRDRLTVADEREHVIFYIAEVLYRVLP
                     GFYDEIALALEKLYGVPAETIELPVLIRFGTWVGGDMDGNPDVNAKTIRETLARQQQV
                     IVNAYFEECQRLAQILSQSASRIGVSPALTQRIERYMTLVPGAQVSTPARHDRMPYRV
                     FLGQVCERLRCTYEGRAQAYENPRQLLDDLGLIAVSLAGNRGRNAGLQLVRRLIRRVE
                     TFGFHFATLDVRQHAHIHHRVLAQGLDDARWLQRPGAERHDALVAAIERDLGPRGELD
                     ALGKRTVAVFEAIVQARHRYGRDAIGFYVVSATQSADDLLAPLLIARWAQAYDKTSGE
                     VALDLCPLFESIESLGACAGVMRRLLDDPLYCRHLESRGRRQCVMVGYSDSNKEGGLC
                     ASRVAVYRAEQSLAATLTDANERHVIFHGRGGSVARGGGRIDALVRAAPPETVNGVLR
                     LTEQGEVISQSYGLRPIALRTLERAFSALALGTHAAQRGTAAPVPAALIEAAQQLADT
                     SREAYRQLVYGQGGFFDYFQAATPIDAIERMQIGSRPAWRDEARSFGALRAVPWVFAW
                     SQTRCLLPGWYGAGSGFAALLATHGIDFVRDACGQWAFLRTLLDDVEAMLARTDLDIA
                     RAYDALYKGGEAPFAALAAEYSRATEAVLTIKGHTALLDGDATLQRAIELRNPYVDPM
                     NLMQVDLLARWRAGGRKDRELFEALLVSISGIAQGLQTTG"
     gene            complement(2693..3313)
                     /gene="fixJ"
                     /locus_tag="PKN91_09620"
     CDS             complement(2693..3313)
                     /gene="fixJ"
                     /locus_tag="PKN91_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator FixJ"
                     /protein_id="HNS28371.1"
                     /translation="MKNETPTVFIIDDDDAARSSLRLLLKSYGLETQLCASAEEFLAN
                     HDPDRPGCIVLDVRMPGMSGIELQQQLNARGATIPIVFITGHGDVPMAVEAMQAGAFD
                     FLQKPFDDNDLIARVQRAIEHNATQRARHHTRQDTRERLGTLTPRERDVLDLVVVGKA
                     NKVIAADLGLSQRTVEIHRARVMEKMDAASLAQLVRMVLDLEREGD"
     gene            complement(3310..4434)
                     /locus_tag="PKN91_09625"
     CDS             complement(3310..4434)
                     /locus_tag="PKN91_09625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS28372.1"
                     /translation="MNTPTPADAQALLREAQEIAQLGNTEFFVPDNGVNYWSPQVYRM
                     LGIDPTEAPSHLRIFEGVHPEDRDRCLLAWSRAIRGTGRLDLSYRYVRPDGTVRHIDA
                     RYRLSRTAGGALRAVGTLHDVTERRTAEEAARRAQARLADVGRLATLGEIAAALSHEL
                     NQPLSAIVNYARACDRLLEAPQPDVAEVKAALGEISSQAMRAGEIIHRLRGMVRHRDT
                     LRTVVSANDLVRDLLALLEMDTRASGCALRLELAPDLPLVLVDGVQIQQVIVNLVHNA
                     AEALLVAGEPGGEVVIRTARSDDGQVVMSVADNGPGLAPGAEARVFEPFFTTKADGTG
                     LGLPISRSILDAHHSQLTYQANTPRGVRFSFTLPVAPELS"
     gene            4494..6071
                     /locus_tag="PKN91_09630"
     CDS             4494..6071
                     /locus_tag="PKN91_09630"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HNS28373.1"
                     /translation="MTCRLLPGFAPLLLPLLAACQTLDLDYPREDSVAIVDGNPTMVH
                     ELFPAMQRAGEGESGFHMLADGVGAFVARVYLVRAAERSLDLQYYMINADDTGRLLVG
                     ELLDAADRGVRVRILLDDIYAAPNDRAIATLDAHPHIEVRLFNPWTRRSGVVGRAVDF
                     LLNPGRLNRRMHNKLFAADNHAVIVGGRNIGDEYFDLKTQMNFRDLDVLGIGPVAAEA
                     SQSFDDYWNSKWAVPAAALTEQDPTEADMRAAREALGAHRERLADSRYVQALEGSQLA
                     QQLDARTLPLEFGKARVLVDDPSKMEAEAGDRAGFLIEELQQSTTDTSHELLVSSPYF
                     VPGKAGVEVLTGLAGKGVAISVMTNSLAANDVAVVHSGYARYRAPLLRGGVRLHELRV
                     TSELSSRRKNRRRFGSEYVSLHAKYLIIDQRKLFVGSINFDPRSIERNTEIGILIESE
                     ALAAQLRRIYRLATGKSLSYEVTLRDGPDAQNDALIWTGENLDGELRFTHEPDTSRWK
                     RFSAWFLGLLPFLESQI"
     gene            6079..7107
                     /locus_tag="PKN91_09635"
     CDS             6079..7107
                     /locus_tag="PKN91_09635"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNS28374.1"
                     /translation="MNRRSALAVAGAVAIAGAGALVARRAWRTRSAPQVPAGAPVGPF
                     GRDSTAEEVTAGLDLTGKVALVTGVSSGLGAETLRVLTLRGAHVLGTARTREKAVAAC
                     AAAGGRATPLALELTDFESVAACADEVRRLGTPLDMLVCNAGVMALPRLEQVYGIEKH
                     FVTNHLGHFVLVNRLLDAVLSAREGRVVVVSSRGYLWAPPGGIEFDNLSGARGEYDPN
                     RAYGQSKLANGLFSLELARRLRGTGATSNSVHPGIIRTNLGRHFSPWKKWLGEVIGWS
                     FMKSIPAGAATQCYVATSPRLAGASGYYFANCNPEVPGGHMEDRELAARLWDVSMELT
                     RAYLPAAA"
     gene            complement(7076..8320)
                     /locus_tag="PKN91_09640"
     CDS             complement(7076..8320)
                     /locus_tag="PKN91_09640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS28375.1"
                     /translation="MTPSQSEFRRGWTVLVASSLGVAFGASPIPYNSLGAFTLPIAEE
                     FGWGRGDVQLGVLWYTIAVVCIVPLVGALADRFGVRRMALLTLAAFGVCFALIGLTPG
                     SLVVFYGLWFFAGLLGGGSTPVTWTRAVNSWFVVNRGLALAITLLGTGITAAFLPTLT
                     IWLIERFGWRWAYFGIALLPLAIALPIAIRWFREPPEKAPAGGGGLARRDEPGFTLRE
                     ALRNYRFWVMVVSILLVATGIGGTITNLVPLLVDRGFTPIEAGKTAGAIGVSIIFGRL
                     FAGFLMDRFWAPGVVFPMLALPALACMILMGQNLGVGMATVAAVLVGFASGAETDLVA
                     FMSARYFGLKHYGRIYGVQYAVFGFASGIAPALFGGMYDRTGSYDAILVIAAIAFGIG
                     AMLMLTMGRYPVFTPPPADTPA"
     gene            complement(8317..>8729)
                     /locus_tag="PKN91_09645"
     CDS             complement(8317..>8729)
                     /locus_tag="PKN91_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS28376.1"
                     /translation="PTNSLLPRLDLANDRQLYLALAGPAWLAGLLAARAMALASRPRR
                     IAVGAALAGLLLCLAVSTAQRNAVYDSEIAFWADVAAKSPANARAAGNLGYALARAGR
                     PVEAEALFRRALALDPQYTRAAINLMLLQDGTLE"
BASE COUNT         1405 a         2944 c         3080 g         1300 t
ORIGIN      
        1 cgccgcgctg cgggacgacg tacacgccct cggcgggctg gtcggcgaga tcctgcgcga
       61 gcagggcggt gacgcgctgt tcgagatcgt cgagcgcgac cgcgtactcg cgatcgcccg
      121 ccgcgccggt gaggccgacg cggccgccga gctcgaggtg cgcgtgcgcg gccggccgcc
      181 ggccgaggcg cgggatctgg tgcgggcctt ctcggcctgg ttccaggccg tcaaccttgc
      241 cgagaaagtc caccgagtcc gtcggcgcaa ggagtacttc ctggccgaca gcgagcgtcc
      301 gcaacccggc ggcgtcgagg atgcgctcgc cacgctgcat gcgggaggcg caagcctgga
      361 agaggtgctc gcgctgctcg cgaagctgcg catcgagccg gtgttcacgg cgcatccgac
      421 cgagtccacg cgccgcacga tcctgcgcaa gcagcaacgc gtcgcggaga tgctgctcga
      481 tcgcctcgat ccgacgctga caccgcagga aacgcgcgcc ctctgggggc gcgtgcgcac
      541 ggagatgacc acggcgtggc agacggagga ccacccgcgc gaccggctca ccgtcgccga
      601 cgagcgcgag catgtgatct tctacatcgc cgaagtgctg taccgcgtcc tgccggggtt
      661 ctacgacgaa atcgcgctcg cgctcgagaa gctctacggg gtgccggccg agacgatcga
      721 attgccggtg ctgatccgtt tcggcacctg ggtcggcggc gacatggacg gcaatccgga
      781 cgtcaacgcc aagacgatcc gcgagaccct ggcgcgccag cagcaggtga tcgtcaacgc
      841 ctacttcgag gaatgtcagc gcctcgcgca gatcctctcg cagagcgcaa gtcgcatcgg
      901 tgtgagtccg gcgctgacgc agcgcatcga gcgctacatg actctcgttc cgggcgcgca
      961 ggtcagcacg ccggcgcgcc acgaccgcat gccctaccgc gtgttcctcg gccaggtctg
     1021 cgagcgcctc cggtgcacgt acgagggtcg cgcgcaggcc tacgagaacc cgcggcaact
     1081 gctcgatgac ctcggcctga tcgcggtcag cctcgccggc aatcgcggcc gcaacgccgg
     1141 cctgcagctc gtgcggcgcc tgatccggcg ggtcgagacc ttcggctttc acttcgcgac
     1201 gctcgacgtg cgtcagcatg cgcacatcca tcaccgcgtg ctcgcacagg gcctcgacga
     1261 tgcgcgctgg ctgcagcgcc cgggtgcgga gcgccacgac gcgctcgtcg cggcgatcga
     1321 gcgcgatctc ggcccgcgcg gcgagctcga tgccctcggc aagcgcaccg tcgccgtatt
     1381 cgaggcgatc gtgcaggcgc ggcaccgcta cggccgcgac gccatcggct tctacgtcgt
     1441 cagcgcgacg cagtcggccg atgacctgct cgcgcccctg ctgatcgccc gctgggccca
     1501 ggcctacgac aagacgagcg gcgaggtggc gctcgacctg tgcccgctat tcgagagcat
     1561 cgaatcgctg ggcgcgtgcg ccggtgtcat gcgccggctg ctggacgacc cgctctactg
     1621 ccgtcatctc gagtcacgcg gacgacgtca gtgcgtcatg gtcggctatt ccgactccaa
     1681 caaggaaggc ggactgtgtg cgtcgcgcgt cgcggtctac agggccgagc agagccttgc
     1741 cgcgacgctg accgacgcca acgagcgcca cgtgatcttc cacggccgtg gcggcagcgt
     1801 cgcgcgcggc ggcgggcgta tcgacgcgct ggtgcgcgca gcgcctcccg agacggtgaa
     1861 cggcgtgctg cgcctgaccg aacagggcga ggtgatcagc cagagctacg gcctgcggcc
     1921 aattgcgctg cgcacgctcg agcgggcctt cagcgcactt gcgctcggga cgcacgccgc
     1981 gcagcgcggc actgccgcgc cggtgcccgc ggcactgatc gaggccgcgc agcaactcgc
     2041 ggatacgagc cgcgaagcgt accggcagct ggtctacggg cagggcgggt tcttcgacta
     2101 cttccaggcc gccacgccga tcgacgcgat cgagcggatg cagatcggtt cgcggcccgc
     2161 gtggcgggac gaggcgcgtt cattcggggc gctgcgggcg gtgccctggg tgttcgcgtg
     2221 gtcgcagaca cgctgtctgc tgcctggctg gtacggcgcg ggcagcggct tcgcggcgct
     2281 gctcgccaca cacggcatcg acttcgtgcg cgacgcctgc gggcagtggg cgttcctgcg
     2341 cacgctgctc gacgacgtgg aggcgatgct ggcacgcacg gacctcgata tcgcgcgcgc
     2401 atacgatgcg ctgtacaagg ggggcgaggc gcccttcgcg gcgcttgcgg cggagtactc
     2461 gcgggccacg gaggcggtgc tcacgattaa gggccacacg gccctgctcg acggcgatgc
     2521 aacgctgcag cgggcgatcg agctgcgcaa cccctatgtc gatccgatga acctgatgca
     2581 ggtcgacctg ctcgcgcgct ggcgcgcggg cggccgcaag gaccgtgaac tgttcgaagc
     2641 attgctcgtg agcatcagcg gcatcgcgca gggactgcag accacgggct gatcagtcgc
     2701 cctcgcgctc gaggtcgagc accatgcgca ccagctgtgc gagcgacgcg gcatccatct
     2761 tctccatgac gcgcgcgcgg tggatctcca cggtgcgctg actcagcccg aggtcggccg
     2821 cgatcacctt gttggccttg ccgacgacca ccaggtcgag cacgtcgcgc tcgcgcggcg
     2881 tgagtgtgcc gagacgctcg cgcgtgtcct ggcgggtgtg gtgccgggcc cgctgggtcg
     2941 cgttgtgctc gatcgcgcgc tgcacgcggg cgatcaggtc gttgtcgtcg aacggtttct
     3001 gcaggaaatc gaaggcgccg gcctgcatgg cctccacggc catcggcaca tcgccgtggc
     3061 cggtgatgaa cacgatcggg atcgtcgcgc cgcgcgcatt cagctgctgc tgcagctcga
     3121 tgccgctcat gccgggcatg cgcacatcga gcacgatgca gcccgggcga tcagggtcgt
     3181 ggttggcgag aaactcctcc gccgatgcgc acagctgcgt ctccaggcca taggacttga
     3241 gcagcaggcg caacgagctc ctggccgcgt catcgtcatc gatgatgaat accgtgggtg
     3301 tttcgttctt catgaaagct ccggcgcgac gggtagtgtg aaggaaaagc gcaccccgcg
     3361 cggtgtgttg gcctggtagg tgagctggct atggtgcgcg tccaggatcg agcggctgat
     3421 gggcaggccg agccccgttc cgtcggcctt ggtggtgaag aaaggctcga acacgcgcgc
     3481 ttcggcgccc ggcgcgaggc ccggtccgtt gtcggccacg ctcatcacca cctggccgtc
     3541 gtcgctgcgc gcggtcctga tcacgacttc tcctcccggt tcgcctgcca ccagcagtgc
     3601 ttcggcggca ttgtgtacga gattgacgat gacctgctgg atctgcacgc catcgaccag
     3661 cacgagaggc agatcgggcg cgagttcgag ccgcagcgcg cagccgctgg cgcgggtatc
     3721 catctccagc aacgccagca ggtcacgcac caggtcgttg gcggacacga cggtacggag
     3781 cgtgtcgcga tggcgcacca tgccgcgcag ccggtggatg atctcgccgg cgcgcatcgc
     3841 ctgcgaggat atctcgccga gcgcggcctt cacctcggcc acgtccggct gcggcgcctc
     3901 gagcaaccgg tcgcaggcgc gggcatagtt cacgatcgcg gacagcggct ggttcagctc
     3961 gtgcgacagc gcggcggcga tctcgccgag cgtggccagc cggccgacgt cggcaaggcg
     4021 cgcctgcgca cgccgtgccg cctcttcggc ggtcctgcgc tcggtcacgt cgtgcagcgt
     4081 gccgaccgcg cgtagtgcgc ccccggcggt gcgcgagagc cggtaacgcg cgtcgatgtg
     4141 gcgcaccgtg ccgtcgggcc gcacgtagcg atagctgagg tccagcctgc cggtaccgcg
     4201 gatcgcccgc gaccaggcca acaggcagcg gtcgcgatcc tcgggatgca cgccctcgaa
     4261 gatgcgcagg tgcgagggcg cctcggtcgg gtcgatgccg agcatccggt agacctgcgg
     4321 cgaccagtag ttcacgccgt tgtcaggcac gaagaactcg gtgttgccga gctgtgcgat
     4381 ttcctgcgcc tcgcgcagca gggcctgtgc gtcggcgggg gttggggtgt tcaaccggga
     4441 taacctcgtt tgcgatacgc tagtgtgtcc gcgcatgagt gtggagggca acattgacgt
     4501 gccgactgct gcctggcttc gcgccattgc tgctgccgtt gctcgcggcc tgccagacgc
     4561 tcgacctcga ctatccgcgt gaagactcgg tggcgatagt cgacgggaac ccaaccatgg
     4621 tccacgaact gttccccgcg atgcagcgcg ccggggaggg cgagtcgggc ttccacatgc
     4681 tggccgacgg cgtcggggca ttcgttgcgc gcgtctacct ggtgcgcgcg gccgagcgct
     4741 cgctcgacct gcagtactac atgatcaacg ccgatgatac cggccggctg ctggttggtg
     4801 aactgctgga tgccgccgac cgcggcgtgc gcgtgcgcat cctgctcgac gacatctatg
     4861 cggcgccgaa cgatcgggcg atcgcaaccc tcgacgcgca tccgcacatc gaggtgcggc
     4921 tgttcaatcc atggacgcgc cgcagcggcg tggtcggccg cgcggtcgat ttcctgctga
     4981 atcccggcag gctgaatcgt cgcatgcaca acaagctgtt cgcggcggac aaccacgccg
     5041 ttatcgtcgg cgggcgcaac atcggcgacg agtacttcga cctgaagacg cagatgaact
     5101 tccgcgacct cgacgtgctc ggcatcgggc cggtcgcggc ggaggcatcg cagtcgttcg
     5161 acgattactg gaacagcaag tgggcggtgc cggcggccgc actgaccgaa caggacccga
     5221 ccgaagcgga catgcgcgcc gcacgcgagg cgctgggcgc gcatcgcgag cgcctcgccg
     5281 actcgcggta cgtgcaggcg ctcgagggct cgcagctcgc gcaacagctc gatgcgcgca
     5341 cgctgccgct cgagttcggc aaggcccggg tgctcgtgga cgatccctcg aagatggagg
     5401 ccgaagcggg cgatcgggcc ggcttcctga tcgaggagct gcagcagagc accacggata
     5461 ccagccacga gctgctggtt agctcgccgt acttcgtgcc gggcaaggcg ggagtcgaag
     5521 tgcttaccgg gcttgccggc aaaggggtgg cgatctcggt catgaccaac tcgctcgcgg
     5581 ccaatgacgt ggcggtggtg cacagcggct acgcgcgcta ccgggcgccg ctgctgcgag
     5641 gcggcgtgcg cctgcacgaa ctgcgcgtga cctcggagct gagctcgcgc cgcaagaacc
     5701 ggcgccgctt cggttccgag tacgtgagcc tgcacgccaa gtacttgatc atcgaccagc
     5761 gcaagctgtt cgtcggatcg atcaatttcg atccgcgctc catcgagcgc aacaccgaga
     5821 tcggcatact gatcgagagc gaggcgctcg ctgcgcagct gcggcgtatc taccggctcg
     5881 cgaccgggaa gtccctgagt tacgaggtga ccctgcgtga cggtcccgat gcgcagaacg
     5941 acgccctgat atggaccggc gagaatctcg atggcgagtt gcgtttcacg catgagcccg
     6001 atacctcgcg gtggaagcgc ttctcggcgt ggttcctggg actgttgccg ttcctcgaga
     6061 gccagatctg agcacgcgat gaaccggcgc agtgcactgg cggtggcagg ggcggtggcg
     6121 atagccggtg ccggcgcgct cgtcgcgcgg cgtgcctggc gcacgcgcag cgcaccgcag
     6181 gtgcccgcag gcgcgccggt gggtccgttc ggcagggact ccacggccga ggaagtgacc
     6241 gccgggctgg atctcaccgg caaggtggcg ctggtcaccg gcgtcagctc cggcctcggc
     6301 gccgagaccc tgcgcgtgct gacgctgcgc ggggcccacg tgctcggcac ggcgcgcacg
     6361 cgcgagaagg ccgttgcggc ctgcgccgcg gcgggcggac gcgccacgcc gctcgcgctc
     6421 gaactgaccg acttcgagtc ggtggccgcc tgcgccgatg aggttcgccg cctcggcaca
     6481 ccgctcgaca tgctcgtctg caacgccggc gtgatggccc tgccgagact cgagcaggtg
     6541 tacggcatcg agaagcattt cgtgaccaac catctcggcc acttcgtgct cgtcaatcgc
     6601 ctgctcgacg ccgtgctgtc agcccgcgag ggcagggtgg tggtggtgtc gagccgcggc
     6661 tacctctggg cgccgcccgg cgggatcgaa ttcgacaatc tgtccggcgc gcgcggcgaa
     6721 tacgatccga accgcgccta cgggcagagc aagctggcca acgggctgtt ctcgctcgaa
     6781 ctcgcgcggc ggctgcgggg caccggcgcg acctcgaatt ccgtgcatcc gggcatcatc
     6841 aggacgaacc tcggccggca cttcagtccc tggaaaaaat ggctcggcga ggtgatcggc
     6901 tggagcttca tgaaaagcat tcccgcgggc gctgcgacgc aatgctatgt cgcaacgtca
     6961 ccgcgcctcg ctggcgccag cggatactat ttcgcgaact gcaatcccga ggtgccgggc
     7021 ggacacatgg aggaccgcga actcgcggca cggctgtggg acgtctcgat ggagctcacg
     7081 cgggcgtatc tgccggcggc ggcgtgaaga ccgggtacct gcccatcgtg agcatcagca
     7141 tcgcgccgat gccaaaggcg atcgccgcaa tgaccaggat cgcatcgtag ctgccggtac
     7201 ggtcgtacat gccaccgaac agcgccgggg cgatcccgga tgcaaagccg aacacggcat
     7261 actgcacgcc gtagatgcgc ccgtagtgct tcaggccgaa atagcgggca ctcatgaatg
     7321 cgacgagatc ggtctcggcg cccgaggcga agccgaccag caccgcggcc acggttgcca
     7381 tgccgacgcc gaggttctgg cccatcagga tcatgcaggc gagcgccggc agcgccagca
     7441 tcgggaagac cacgcccggc gcccagaagc gatccatcag gaagcccgcg aacaggcgac
     7501 cgaagatgat gctgacgccg atcgcgcccg cggtcttgcc cgcctcgatc ggcgtgaagc
     7561 cgcggtccac cagcagcggc acgagattgg tgatcgtgcc gccgatgccg gtcgcgacca
     7621 gcaggatgga cacgaccatg acccagaagc ggtagttgcg cagtgcctcg cgcagcgtga
     7681 aacccggctc gtcgcggcgg gcgagcccgc cgcccccggc cggcgccttc tcgggcggct
     7741 cgcgaaacca gcggatcgcg atcggcagcg cgatggcgag cggcagcagc gcgatgccga
     7801 agtaagccca gcgccagccg aagcgctcga tcagccagat cgtcagcgtc ggcaggaagg
     7861 ccgccgtgat gccggtgccg agcagcgtaa tcgcgagcgc caggccgcga ttgaccacga
     7921 accagctgtt gaccgcgcgg gtccaggtca ccggcgtcga accgccaccc agcagaccgg
     7981 cgaagaacca cagcccgtag aagacgacga gcgaacccgg cgtgaggcct atcagggcga
     8041 aacagacgcc gaatgcggcc agcgtcagca gcgccatgcg ccgcacgccg aagcggtccg
     8101 ccagtgcgcc gaccagcggc acgatgcaga ccaccgcgat cgtgtaccac aacacgccca
     8161 gctgcacgtc gccccggccc cagccgaatt cttcggctat cggcagcgtg aaggcaccga
     8221 gggagttgta ggggatgggc gaagcgccga aggcgacacc gagcgaggag gcgaccagca
     8281 cggtccagcc gcgccggaat tcggattggg atggcgtcat tccagggtgc cgtcttgcaa
     8341 cagcatgagg ttgatggcgg cacgagtgta ctgggggtcg agcgcgagtg ctcgacgaaa
     8401 cagcgcctcg gcttccacag ggcggccggc gcgcgcaagt gcatagccca ggttgcccgc
     8461 ggcacgtgca ttggccggcg acttggccgc gacatcggcc cagaatgcga tctcgctgtc
     8521 gtagaccgcg ttgcgctgcg cggtcgacac cgcgaggcac agcagcagcc cggcgagggc
     8581 ggcgccgact gcgatgcgcc gcgggcggga ggcgagcgcc atggcgcgcg ctgccagcag
     8641 gcccgcaagc caggccggcc cggcgagcgc gaggtagagc tggcggtcat tggcgagatc
     8701 gagccgcggc agcagcgaat tggtgggcg
//
LOCUS       DAOQVY010000103         8722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_8956_length_8722_cov_5.958348, whole genome shotgun sequence.
ACCESSION   DAOQVY010000103 DAOQVY010000000
VERSION     DAOQVY010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8722
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8956_length_8722_cov_5.958348"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PKN91_09650"
     CDS             complement(<1..842)
                     /locus_tag="PKN91_09650"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HNS28377.1"
                     /translation="MARADPRPIPEVLAAVDLGSNSFHLVVARYIHGQLTVIDRLREV
                     VRLGAGVSESGRLDRDVAARALACLERFGQRLRDMHARGVRIVGTNALRRAHRKQAFL
                     ERAREALGHPIEIVSGMEEARLIYAGVAHTSPAEAGRRMVFDIGGGSTEVIIGEALEP
                     IELASLQMGCVNMSERFFPGGRLSPKRLARARLAVELELAPVVARFRQLGWQHVAGSA
                     GTVRAVGECLREMDPAATSITAEGVEALLAAMGEVDDIRDLEFAAIDADRAGVFAGGA
                     AVLAG"
     gene            complement(854..1195)
                     /gene="erpA"
                     /locus_tag="PKN91_09655"
     CDS             complement(854..1195)
                     /gene="erpA"
                     /locus_tag="PKN91_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNS28378.1"
                     /translation="MSNEVSPLIFTDAAARKVGDLIRGEGNPKLMLRVFVQGGGCSGL
                     SYGFEFDEEIQDGDTCVENQGVKLLVDPMSVQYLTGAEIDYRENLDGSQFVIRNPQAT
                     TTCGCGSSFSA"
     gene            complement(1255..1686)
                     /locus_tag="PKN91_09660"
     CDS             complement(1255..1686)
                     /locus_tag="PKN91_09660"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNS28379.1"
                     /translation="MFGRDRNARLQVDTLIGKSARIDGDLAFEGGLHLDGRVAGNVLG
                     EGAAGNTLSVSEQGRIEGSVAVPTVVLNGTVSGDIRAATRLVLGPRARVEGSVQYGRI
                     EMALGAEIRGRLMPLGADRDPQVVDDGAAVTGEYRVTEAAS"
     gene            complement(1686..2405)
                     /locus_tag="PKN91_09665"
     CDS             complement(1686..2405)
                     /locus_tag="PKN91_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28380.1"
                     /translation="MSVSEPKVVLRSYAPARRRWVAGVLVLLGIFALYVAYEYGRFDG
                     GYDRLTTSQREAEHRIEVGRLEAQNRELRIKVAEFETTAVGQANERAEVARAIGELQE
                     RVARQAQELAFYEGIVVQGGNAPEVKVQQLRISATDEPRKFRLRITLVQPVRPDRTVT
                     GVVTVRVDGEREGGPITLDLAGLTDGALGEIPYSFRYFANIDPEVTLPEGFSPAHVQV
                     EVRSNRKGISPATQTLDWSVE"
     gene            complement(2402..2920)
                     /locus_tag="PKN91_09670"
     CDS             complement(2402..2920)
                     /locus_tag="PKN91_09670"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016144233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HNS28381.1"
                     /translation="MIDAQGFRANVGIVLMHASGRVFLGRRVGGRGWQFPQGGMRQGE
                     DVETALMRELQEEIGLDPGDVVVVGRTDRWLRYRLPAKFVRRNQHPVCIGQKQCWFLL
                     KLANEDARFRFDLTGEPEFDQWRWAEYWEPVREVIRFKRPVYRRALHELGALAFPGGM
                     PAYPEWWREVAS"
     gene            2993..3982
                     /locus_tag="PKN91_09675"
     CDS             2993..3982
                     /locus_tag="PKN91_09675"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNS28382.1"
                     /translation="MSPAGTLLRFVLLVACATLALAASLLYGTAGLGAQDALQALLGG
                     ADETARSIVLDVRLPRTLAAFGVGSVLALAGVLLQALFRNPLADPYVLGVSGGAAIGA
                     LLALIAGAGAAATRSGAFMGALGAIGVVAAMGRGGGMTRLLLTGVVVASACGAGVSVL
                     LALADSGQLRGMVFWLAGDLGWADDAGASGLAALLALGLALAFARPLNVLAAGELRAR
                     TVGLDIRLWRWVIFLASAVLTSTAVITAGTIGFVGLVTPHAVRLAFRSADHRIVAPGA
                     ALLGGTLVVAADFAARTIAAPRQLPVGAIMALVGAPLFLALLRATVSERSGRQ"
     gene            complement(3957..5138)
                     /locus_tag="PKN91_09680"
     CDS             complement(3957..5138)
                     /locus_tag="PKN91_09680"
                     /inference="COORDINATES: protein motif:HMM:NF016243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HNS28383.1"
                     /translation="MEPPGRPPRRPPVSPPARRRGQRDLPPEIDESGENRILRARLVE
                     LAAAAARNEALLAKTQERELDLLRAGSLAELLERIVIGLREAYQLDRVTLLLNDPQHE
                     LRHLASGEHVGHELLAHVRHEDRLDAAALALGGLTKPWLGSRLPADVVRALRSNAVGG
                     SYAFLPLPRGERRLGMLVFESRDPARFTPDLASDFLGHLGVVAAICLENALNRSRLLR
                     TGVTDFLTGFHNRRYLNARLREELARAQRTAGAVGLLMIDVDYFKRINDTRGHLAGDA
                     VLKEISRRIASQIRSSDTGARFGGDEFAVLLGNGRAGDLERLAGRLTTVMNATPVTVD
                     GEGSERVTLSIGGALAEPAPDERDYRALAERLMAEADAALYRVKSAGRDGFVIADRIV
                     R"
     gene            complement(5143..6267)
                     /locus_tag="PKN91_09685"
     CDS             complement(5143..6267)
                     /locus_tag="PKN91_09685"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HNS28384.1"
                     /translation="MSLEALRQHLNDLDARLLELVADRQQTSREIARVKRATGHATRD
                     YGREREVIMGARDRASALGISPDLAETFMRLLIRSSLTTQERASVVAQNAGTGQRALV
                     IGGAGKMGRWFAEFMQSQGYEVEISDTSDATLAGTTLAGVPRVDWHAIELTHDFIVVA
                     TPLSATDAVLRELALRRPTGVVFDLGSLKSPLRGGLMALRSHGVKVTSLHPMFGPDTE
                     LLSGRHVIFVDLGVPEALARARELFAPTMVERVVMGLDEHDRLIAYVLGLSHALNIAF
                     YNALADSGEDAPRLAKLSSTTFDAQLDVATRVAQDSPDLYFEIQSLNDYGAESLERLA
                     KAVEQLRTAVLSQDRAAFTAMMLRGREYLEDRRALAERRA"
     gene            complement(6374..6712)
                     /locus_tag="PKN91_09690"
     CDS             complement(6374..6712)
                     /locus_tag="PKN91_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28385.1"
                     /translation="MLRTALMALALTAVGATAVAGDRDDYRRHDRGNKHYQQYDRHAP
                     PGHYGKHYRKPPRHVHYVPARPVYYRPPPRVYYPARGYYPAAQVYYPVPVHAHGGQPS
                     LGIQLYIPLK"
     gene            6824..7504
                     /gene="pdxH"
                     /locus_tag="PKN91_09695"
     CDS             6824..7504
                     /gene="pdxH"
                     /locus_tag="PKN91_09695"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNS28386.1"
                     /translation="MFPTEFLPDPLPPEPLPIAAAWLAEAVRRADQPNPNAMVLATVG
                     AGGQPSARVVLCKDIVTTPGYVQFFSNYESHKGRDLAENPRAAIVMHWDHLHRQVRIE
                     GIVQKAPADESDAYFATRPWQRQVGAWASRQSRPVDSRATLLADLAKTAARFGAPVPG
                     PEDDPAPPGIVIPRPPHWGGYCLWASAVELWVEGESRIHDRARWERTLDAAGAAAPGA
                     WRATRLQP"
     gene            7541..8290
                     /locus_tag="PKN91_09700"
     CDS             7541..8290
                     /locus_tag="PKN91_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28387.1"
                     /translation="MLLLVLVAVASQAWLDRTSTRDWSEPLWVGIFPIGADDDPVTEA
                     YLGALQPTAFEAIEQFFEAEAREYGIDVERPVHIELYPNPRQHPPELPAQAGWLRTAA
                     WSLAMRWYAWRVTAAIEGAPPNIRLFVQYHAPQRLEVLPHSLGLSKGLMGVVHAYADR
                     GHEGGNNVVIAHELLHTLGATDKYDLATLLPLYPDGYAEPAREPRHPQPFAEIMAGRR
                     ALSTREAEMPASLAETLIGPATAREIAWSAP"
     gene            8287..>8722
                     /locus_tag="PKN91_09705"
     CDS             8287..>8722
                     /locus_tag="PKN91_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS28388.1"
                     /translation="MSRTILAVRGLDIRAGERLLVRGLDLDVRPGECIALLGRNGTGK
                     SLTLHTLAGLRAPGGGRIELDGRPLDSFARRAIAQHIGLLLQDLETGHSETALETVLV
                     GRHPHLSPWQWEREEDRRLARSMLARVGLDLAGPRKMATLSGG"
BASE COUNT         1295 a         3093 c         3007 g         1327 t
ORIGIN      
        1 cccgcgagca cggcggcacc gccggcaaaa acaccggccc ggtccgcatc gattgccgcg
       61 aactcgagat cgcgtatgtc atcgacttcg cccatcgcgg cgagcagcgc ctcgacgccc
      121 tcggccgtga tcgaagtcgc ggcgggatcc atctcgcgca ggcattcgcc gaccgcgcgc
      181 acagtgcccg cgctgccggc gacgtgctgc cagccgagct ggcgaaagcg cgccaccacc
      241 ggtgcgagtt cgagctccac ggcgaggcgc gcccgcgcaa ggcgtttcgg cgacaggcgt
      301 ccgcccggga agaaacgctc gctcatgttc acgcagccca tctgcaggct cgcgagctcg
      361 atcggctcga gggcctcgcc gatgatcacc tcggtgctgc cgccaccgat gtcgaacacc
      421 atgcgccggc ccgcctccgc aggcgaggtg tgcgcgacac ccgcatagat gagccgtgcc
      481 tcctccatgc cggagacgat ttcgatggga tggccgagtg cctcgcgcgc gcgctcgagg
      541 aaggcctgct tgcgatgtgc gcgccgcagc gcgttggtgc cgacgatccg cacgcccctg
      601 gcgtgcatgt cgcgcagtcg ctggccgaag cgctcgagac aggcgagcgc ccgcgcggcg
      661 acatcgcgat cgagccggcc gctttcgctg acaccggcgc cgagccgcac gacctcgcgc
      721 agccggtcga tgacggtcag ctggccgtgg atgtaacgcg cgacgacgag gtggaaggaa
      781 ttcgagccga gatcgacggc cgccaggacc tcggggatcg gtcgggggtc cgcccgcgcc
      841 acggcggccc ggatcaggcc gagaaggacg aaccgcagcc gcaggtcgtg gtggcctggg
      901 gattacggat cacgaactgg gaaccatcga ggttctcgcg ataatcgatt tccgcgccgg
      961 tgaggtactg cacgctcatc ggatcgacca gcagcttcac gccctggttc tcgacgcagg
     1021 tatcgccgtc ctggatttcc tcgtcgaact cgaagccata ggacaggccc gagcagccac
     1081 caccctgcac gaacacgcgc agcatcagct tcggattgcc ttcgccgcgg atcaggtcgc
     1141 cgaccttgcg ggctgcggca tcggtgaaaa taagcgggct gacttcgtta ctcatgtctg
     1201 caaaaaaccg tgaaccaaca atagtttacg cgcgcaggcg ccggcggtca aagcctagct
     1261 cgcagcctcg gtgacccggt actcgccggt gactgcggcg ccgtcgtcga ccacctgcgg
     1321 gtcgcgatcg gcaccgagtg gcatcaaccg gccgcggatt tcggcgccca gcgccatttc
     1381 gatgcggccg tactgcacgc tgccctcgac ccgggcccgg gggcccagca cgagccgagt
     1441 cgcggcgcgg atgtcgccgc tgacggtgcc attcagcact acagtgggga cggccaccga
     1501 accttcaata cggccctgct cgctgacgga cagagtattg cccgcggcgc cctcgcccag
     1561 cacgttgccg gccacgcgac cgtcgaggtg caggccgccc tcgaaggcga ggtcgccgtc
     1621 gatgcgcgcg gacttgccga tcagcgtgtc gacctgcagg cgtgcgttgc gatccctgcc
     1681 gaacatcact ccacactcca atccagtgtc tgggttgcgg gggaaatccc cttgcggttc
     1741 gagcgtacct cgacctgcac atgcgcgggg ctgaaacctt ccggaagtgt gacctccgga
     1801 tcgatgttgg cgaaatagcg gaacgaatag ggaatttcgc ccagtgcgcc gtcggtgagc
     1861 ccggcgagat cgagcgtgat cggcccgccc tcgcgctcgc cgtcgacccg cacggtcacg
     1921 actccggtca cggtccggtc cggcctcacg ggctgcacga gggtgatgcg caggcggaac
     1981 ttccgcggct catcggtggc cgaaatgcgc agctgctgga ctttcacttc cggggcattg
     2041 ccgccctgca cgacgatgcc ctcgtagaac gcgagttcct gcgcctgcct cgcgacccgc
     2101 tcctgcagct cgccgatcgc cctcgcgact tcggcacgtt cgttggcctg gcccaccgcg
     2161 gtggtttcga actcggccac cttgatgcgc agctcgcggt tctgcgcctc caggcgtccg
     2221 acctcgatac ggtgctcggc ctcgcgctgc gatgtggtca gccgatcgta gccgccgtcg
     2281 aagcgcccgt actcgtaggc gacatagagc gcgaagatgc cgagcaatac cagcacgccg
     2341 gcgacccagc gccggcgcgc cggcgcgtag ctgcgcagga cgaccttcgg ttccgagacg
     2401 ctcacgaggc gacctcgcgc caccattcgg ggtaggccgg cattcccccg ggaaaggcca
     2461 gcgcaccgag ttcatgcagc gcgcggcggt acaccggccg cttgaaccgg atgacctcgc
     2521 gcaccggctc ccagtattcg gcccagcgcc attggtcgaa ttcgggctcg ccggtcaggt
     2581 cgaaacggaa gcgggcatcc tcgttggcga gtttcagcag gaaccagcac tgcttctgcc
     2641 cgatgcacac cgggtgctgg ttccggcgca cgaacttcgc gggcaaccgg tagcgcagcc
     2701 agcgatcggt gcgcccgacg acgaccacgt cgccgggatc gaggccgatc tcctcctgca
     2761 gctcgcgcat cagcgccgtc tcgacgtcct cgccctggcg catgccacct tgcggaaact
     2821 gccagccacg gccgccgacg cgccgtccga ggaacacgcg gccgctggcg tgcatcagca
     2881 cgatgccgac attggcgcga aatccctggg cgtcgatcac atccgtcatg cgtctctctt
     2941 ttacctgcgg gccgcaggcg gaacaagacg caaggctgct acgcttgcgc acgtgagtcc
     3001 cgcgggaacc ctgctgcgct ttgttctgct cgtcgcttgc gccaccctgg cactcgctgc
     3061 gtcgctactg tacggcaccg cgggcctcgg tgcgcaggat gcgctgcagg cactgctcgg
     3121 cggcgccgac gaaaccgcgc gcagcatcgt gctcgatgtg cgcctgcctc gcacgctcgc
     3181 cgccttcggc gtcggcagcg tacttgcgct cgcgggagtg ctgctgcagg cgctgtttcg
     3241 caatccgctc gccgacccct atgtgctcgg cgtgtccggc ggcgcggcga tcggcgcact
     3301 gcttgcactg atcgccggcg cgggcgcggc ggcgacgcgc agcggcgcgt tcatgggcgc
     3361 gctcggggcg atcggcgtgg tcgccgccat gggccggggc ggcggcatga cccggctgct
     3421 gctgaccggc gtggtggtcg cgagcgcatg cggcgccggc gtttcggtgc tgctcgcgct
     3481 ggccgacagc ggccagctgc gcggcatggt gttctggctc gccggcgatc tcggctgggc
     3541 ggacgatgcg ggcgcgagcg gactggccgc gctgctcgcg ctcggcctcg cgctcgcctt
     3601 tgcgcgtccg ctcaatgttc tcgcggcggg cgagctgcgc gcgcggaccg tggggctcga
     3661 catccggctg tggcgctggg tgattttcct cgccagcgcg gtgctgacct cgaccgcggt
     3721 gatcacggcc ggcacgatcg gcttcgtcgg cctcgtgacg ccgcacgcgg tgcggctcgc
     3781 ctttcgcagc gcggatcacc gcatcgtcgc accgggcgcc gcgctgctcg gcggcaccct
     3841 ggtcgtggct gccgatttcg cggcccgcac gatcgccgcg ccccggcaac tgccggtcgg
     3901 cgcgatcatg gcgctggtcg gcgcgccgct gttcctcgca ctgctgcgcg cgaccgtcag
     3961 cgaacgatcc ggtcggcaat aacgaatccg tcgcgccctg cgctcttgac ccggtagagc
     4021 gccgcgtccg cctcggccat cagccgctcg gcgagtgcgc gatagtcacg ctcatccggc
     4081 gccggctcgg cgagcgcccc gccgatcgac agcgtgacgc gttcgctccc ttcaccgtcg
     4141 accgtgacag gcgtggcgtt catcaccgtg gtgaggcgcc ccgcgaggcg ctcgaggtcg
     4201 ccggcgcgtc cgttgccgag cagcaccgcg aactcatcgc cgccgaaacg cgcgcccgtg
     4261 tcgctcgagc ggatctggga ggcgatgcgc cgcgagatct ccttcagtac cgcatcgccg
     4321 gcaaggtgtc cgcgggtgtc gttgatgcgc ttgaagtaat cgacatcgat catcagcagg
     4381 ccgaccgcgc cggcggtacg ctgcgcacgc gccagctcct cgcgcaggcg tgcgttcagg
     4441 tagcggcgat tgtggaagcc ggtcaggaag tcggtaacgc cggtgcgcag caggcgcgag
     4501 cggttgagcg cgttctcgag gcagatcgcc gccaccacgc cgagatggcc gaggaagtcg
     4561 ctcgcgagat cgggcgtgaa gcgcgccggg tcgcgactct cgaataccag catgccaagg
     4621 cgccgctcgc cacgcggcag cggcaggaac gcgtagcttc cgcccaccgc gttcgagcgc
     4681 agcgcgcgca ccacgtccgc gggcaggcgg ctgccgagcc agggcttggt gagcccgccg
     4741 agcgcaagcg ccgcggcatc gagacgatcc tcgtggcgta cgtgcgcgag cagctcgtgc
     4801 cccacgtgct cgccactcgc gaggtggcgc agctcgtgct gcggatcatt cagcagcagc
     4861 gtcacgcgat cgagctggta ggcctcccgc aggccgatga cgatgcgctc aagcagttcg
     4921 gccaatgaac cggcgcgcag caggtcgagt tcgcgctcct gcgtcttcgc gagcagtgcc
     4981 tcgttgcgcg ccgccgcggc cgccagctcg accagccggg cacgcaggat gcggttctcg
     5041 cccgactcgt cgatttcggg tggcaggtcg cgctggcccc gccgccgggc cggcggcgat
     5101 acgggcggac gtcgcggcgg tcggcctggc ggttccatcg cctcaggcgc ggcgctccgc
     5161 gagcgcgcga cggtcttcca ggtattcgcg gccacgcagc atcattgccg tgaaagccgc
     5221 gcggtcctgc gacagcacgg ccgtgcgcag ctgctccacg gccttggcga gtctctcgag
     5281 cgattcggcg ccgtagtcgt tcagtgactg gatctcgaaa tagagatcgg ggctgtcctg
     5341 cgccacgcgg gtcgcgacat cgagctgcgc atcgaaggtg gtgctcgaga gcttcgcgag
     5401 gcgcggcgcg tcctcgccgc tgtcggcgag cgcgttgtag aaggcgatgt tgagcgcgtg
     5461 cgagagcccc agcacgtaag cgatcaaccg gtcgtgctcg tcgagcccca tcaccacccg
     5521 ctcgaccatc gtcggcgcga acagctcgcg ggcccgcgcc agcgcctccg gcacgccgag
     5581 gtcgacaaag atcacgtgac gcccggacag cagttcggtg tcgggaccga acatcgggtg
     5641 cagcgaggtg accttcacgc cgtgcgagcg cagcgccatc agcccgccgc gcagcggcga
     5701 cttgagcgag ccgagatcga acacgacgcc ggtcgggcgg cgcagcgcga gctcgcgcag
     5761 cacggcatcg gtggcgctga ggggagtggc gaccacgatg aagtcgtgcg tgagctcgat
     5821 cgcgtgccaa tcgacgcgcg gcacaccggc cagggtcgtg ccggcgagcg ttgcgtcaga
     5881 ggtgtcgctg atctcgacct catagccctg cgactgcatg aattccgcga accagcggcc
     5941 catcttgccc gcgccgccga tcaccagggc acgctggccc gttccggcat tctgggcgac
     6001 gacactcgcg cgctcctggg tggtgagcga ggagcggatc agcaggcgca tgaaggtctc
     6061 ggcgaggtcg ggcgagatgc cgagcgcgct cgcccggtcg cgcgcgccca tgatcacctc
     6121 gcgctcgcgc ccgtaatcgc gggtggcgtg gccggtggcg cgcttgacgc gcgcgatttc
     6181 gcggctcgtc tgctggcggt cggccaccag ctcgagcagt cgtgcgtcga gatcgttgag
     6241 atgctgcctg agagcttcga gtgacatgcc agtgcctgat gcggcggatt gccgacatcc
     6301 tttgtaccgg gcacatcagc ccggcggcaa gcgtgggtca taccgaccca cgcccgccgc
     6361 cgccgcaact gcgtcatttc aacgggatat agagctggat cccgagtgaa ggctggccgc
     6421 cgtgcgcatg gacaggcaca gggtagtaga cctgcgcggc agggtagtag ccgcgcgccg
     6481 ggtagtacac ccgcggcggc gggcggtagt acacgggccg ggccggcaca tagtgcacat
     6541 gcctcggcgg cttcctgtag tgcttgccgt aatggccggg aggggcgtgg cgatcgtact
     6601 gctggtagtg cttgttgcca cgatcgtgcc ggcggtaatc atcacggtca cccgcgacgg
     6661 cggtcgcgcc caccgcggtc agtgccagag ccatgagtgc tgtacgtaac atgactgggt
     6721 cctccgggac ccccacggcg ggagtccgat ctcggggcca gattgccccg gggccaatga
     6781 acccgtgctg aaccgcccgc cgccgaccgc tatcatgcga gcgatgttcc ccaccgaatt
     6841 tctccccgac ccgctgcccc ccgagcccct gccgatcgcg gccgcatggc tcgccgaggc
     6901 cgtccggcgg gccgatcagc cgaaccccaa tgccatggtg ctggcgacgg tgggcgcggg
     6961 aggacagccc tcggcgcgcg tggtgctgtg caaggacatc gtgaccacgc cgggttatgt
     7021 ccaattcttc tccaactacg agtcccacaa ggggcgcgac ctcgcggaga acccgcgcgc
     7081 ggcgatcgtg atgcactggg accacctgca tcgccaggtt cgcatcgagg gcatcgtgca
     7141 gaaggcgccc gccgacgaaa gcgacgccta cttcgccacg cggccctggc agcgtcaggt
     7201 cggcgcctgg gcgagccggc agagtcggcc cgtcgactcg cgcgccacgc tgctcgcgga
     7261 tcttgcgaaa accgccgcac gcttcggcgc tcccgtgccg ggtcccgagg acgatccagc
     7321 gccgcccggc atcgtgattc cgcgcccgcc gcactggggc ggctactgcc tgtgggcgag
     7381 cgccgtggag ctgtgggtcg agggcgaatc gcgcatccac gatcgcgcgc gctgggaacg
     7441 cacgctcgac gccgcgggcg ctgccgcgcc cggcgcatgg cgcgcaacgc gcctgcagcc
     7501 ctgaccgggg cggcgcgtgt ggcgcatcct gcgaattgcg gtgctcctgc tggtgctggt
     7561 cgcagtggcg agccaggcgt ggctcgatcg cacgagcacg cgtgactgga gcgaaccgct
     7621 gtgggtcggc atttttccga tcggcgcaga tgacgacccg gtcaccgagg cctatctcgg
     7681 cgccctgcag cccacggcct tcgaggcgat cgagcagttc ttcgaggccg aagcgcgcga
     7741 gtacgggata gatgtcgaac gcccggtgca catcgaactc tatccgaacc cccggcaaca
     7801 cccacccgaa ctgccggcgc aggcgggctg gttgcgcacc gcggcctgga gcctcgcgat
     7861 gcgctggtat gcgtggcgcg tgaccgccgc gatcgagggc gcaccaccga acatccggct
     7921 gttcgtgcag taccacgctc cgcagcgcct cgaggtgctg ccgcactccc tcgggctcag
     7981 caaggggctg atgggcgtgg tgcacgccta cgccgaccgg ggacacgagg gcggcaacaa
     8041 tgtcgtgatc gcgcacgagc tgctgcacac cctgggcgcc accgacaagt acgatctcgc
     8101 gacactgctg ccgctctacc cggacggtta tgccgagccc gcgcgcgaac cgcgccatcc
     8161 gcagccattc gccgaaatca tggcgggtcg acgcgcgctc tcgacgcgcg aggccgagat
     8221 gcccgcctcg ctcgccgaga cgctgatcgg tcccgcaacc gcccgcgaga tcgcctggag
     8281 cgcaccgtga gccgaacgat cctcgcggtg cgcggtctcg acattcgcgc cggtgagcgc
     8341 ctgctcgtac gcggcctcga cctcgacgtg cgccccggcg agtgcatcgc actgctcggc
     8401 cgcaacggca ccggcaagag cctgacgctg cataccctcg ccggattgcg cgcgccgggc
     8461 ggcggccgca tcgaactcga cgggcggccg ctcgactcgt tcgcgcgccg ggcgatcgcg
     8521 cagcacatcg gcctgctgct gcaggacctc gagaccgggc attcggagac ggcgctcgag
     8581 accgtgctcg tcgggcggca tccgcacctc tcgccctggc aatgggagcg cgaggaggac
     8641 cggcgactcg cccgctcgat gctcgcgcgc gtgggcctcg acctcgccgg accgcgcaag
     8701 atggccaccc tctcgggcgg cg
//
LOCUS       DAOQVY010000104         8618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_9087_length_8618_cov_4.692865, whole genome shotgun sequence.
ACCESSION   DAOQVY010000104 DAOQVY010000000
VERSION     DAOQVY010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8618
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9087_length_8618_cov_4.692865"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /gene="panB"
                     /locus_tag="PKN91_09710"
     CDS             <1..680
                     /gene="panB"
                     /locus_tag="PKN91_09710"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNS28389.1"
                     /translation="VLVGDSLGMVIQGHDTTVPVTMDDIVYHSRTVARGIHRPLLMAD
                     LPFMSYPSKERALENSVRLMQEGGAEMVKLESGAGQIEIVEFLANHDIAVCAHLGLKP
                     QSVHKTGGFRVQGRGAEAAKRMIENAKALESAGADIVLLECVPSELGAEITAALEVPV
                     IGIGAGPGTDGQILVLYDILDITSGRKPRFVKNFMEGAASNLEAVRAYVRAVKDSSYP
                     GPEHGFA"
     gene            689..1540
                     /gene="panC"
                     /locus_tag="PKN91_09715"
     CDS             689..1540
                     /gene="panC"
                     /locus_tag="PKN91_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNS28390.1"
                     /translation="MQVLTTIADVRACVRAYRQAGERIAFVPTMGNLHAGHISLMELA
                     RTGHDRFIASIFVNPMQFGPNEDFAHYPRTPDRDAQMLRDAGCDLMFMPDVAEMYGEG
                     TTAPTRIDVPELTTTLCGAFRPGHFEGVATVVAKLFNIVLPDTAVFGEKDFQQLAVIR
                     RMVADLCFPVTVVGAPTVREADGLAMSSRNQYLTEAERRVAPQIYRALLAATAAIRAG
                     AEEVATIESAGAASLAAHGMQVDYFAVRRRVDLTPPQAADRELVVLTAARLGKARLID
                     NLQVDRP"
     gene            complement(1509..2615)
                     /gene="queG"
                     /locus_tag="PKN91_09720"
     CDS             complement(1509..2615)
                     /gene="queG"
                     /locus_tag="PKN91_09720"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNS28391.1"
                     /translation="MHKADVDTLRLQLLAEATALGFAALGITGIDLTEDERHLDRWLA
                     AGRHGEMDYMARHGTKRSRPAELLPGTLRVISARMDYWPPGGRDAQQVLADPTSAYVS
                     RYALGRDYHKLMRKALAKLAAQLEEWVGPFGHRVFVDSAPVLEKALARNAGLGWIGKH
                     TNLIARDAGSWFFLGEIYTTLELPIDAPASAHCGTCTACIPACPTGAIVAPWQLDARR
                     CISYLTIELHGPIPEELRPLLGNRIYGCDDCQLVCPWNKFAREASHPDFRARHALDTP
                     RLTELFAWSAADFDARMRGSAIYRIGYERWLRNIAVALGNAPTTTEVLAALASRADDP
                     SGLVREHVAWALARHRERAATHPGVTAGRPADCR"
     gene            2656..4131
                     /locus_tag="PKN91_09725"
     CDS             2656..4131
                     /locus_tag="PKN91_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNS28392.1"
                     /translation="MGQPTAIYSAAQVRELDDYAIQTLGIPGYTLMKRAGEAGLRALR
                     ARWPTAHRIGVVCGGGNNGGDGYVIARFARAAGLTVTVLAAVPVEQLRGDAQRAAEDF
                     LASGGQVQEFGAAALRQCEVIVDALLGIGAKGSLRPEVIAIIEAINTSGRPVFAVDIP
                     SGLCADSGMPLGAAVRAEVTITYVGLKCGLFMGEGPEYTGSLLFDDLEVTAPPAPQFA
                     PRLLRASEADIARALPRRRRASHKGSFGHVLVVGGGPGMPGAVRLAGESALRVGAGLV
                     TVAVAPENVASIAAGCPELIVVGLDVAQVGPLLASADVVAIGPGLGRSAWAWEVLGAT
                     VAAGKPLVVDADALNILAEDRTLRADRWILTPHPGEAGRLLGTSTAAVQADRLGSLEQ
                     LCRTYGGTVVLKGAGTLVGRTGDTPQLCERGNPGMATAGMGDVLTGVIAGMLAQLGDP
                     LLAARIGVLVHALAGDAAARGGERGLIARDVINELRTWVNL"
     gene            4128..4625
                     /gene="tsaE"
                     /locus_tag="PKN91_09730"
     CDS             4128..4625
                     /gene="tsaE"
                     /locus_tag="PKN91_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNS28393.1"
                     /translation="MSSVDLEFAAPSAEAMMAFGAALARTRPEAKSGPLLLYLTGELG
                     AGKTTLARGLLAQLGVTGAVRSPSYALVEVHRLADLTVVHADLYRLLDAEELEPLGLR
                     ELYAPDHLWVIEWPERAATALPEPDLHLELSITPAAHRVRPVACGPQGRNWYAAAVEL
                     VRVSH"
     gene            4669..5859
                     /locus_tag="PKN91_09735"
     CDS             4669..5859
                     /locus_tag="PKN91_09735"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNS28394.1"
                     /translation="MRSLVSATLLLFAAALVTDDARGAGRRVDGVRASADGAGARVVV
                     DLTAPADFKLFTLERPRRIVLDLADTALADTVKLPRAEGPITAVRSGVQPGGTLRLVI
                     ETTSSDEPRASRIAPAADGGHRLLVEFGAISPAAANTRRVVAAVHAPKQGARDVVIAI
                     DAGHGGKDPGAIGHGGTFEKTVVLEIARELARRIDEAPGMRAVLIRDGDYYIAHRERI
                     RRARNARADMFLSIHADALRDRGVAGASVYVLSERGATDEAARWLAERENAADLRGGV
                     TLSDKDDVIATVLLDLSQSAAISASMEAAERILASFAGVVPVRKPRVQQAGFLVLKSP
                     DIPSMLIETAYITNAADEKRLRSNAQRRKLANSIVDGVQSYFTRNPPEGTRFAELRRL
                     AAAD"
     gene            5940..7745
                     /gene="mutL"
                     /locus_tag="PKN91_09740"
     CDS             5940..7745
                     /gene="mutL"
                     /locus_tag="PKN91_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNS28395.1"
                     /translation="MPICVLASELIDQIAAGEVIERPASAVKELLENALDAGASRIEI
                     DIERGGVGLIRVRDDGAGIAAAELPLAIARHATSKIASLDDLESVGTLGFRGEALPSI
                     GSVARLRVISRPAGAAQAHEIAVEGGQVGAVKPAAHPPGTTVEVRDLFYNVPARRKFV
                     RSDATETGHIARWVERLALARPDVAFRLRSGTRTLLDAPAIAADEDPAARVDAVLGPE
                     FLARALPVRHAAGPVLIEGWIGAPTASRAQPDSQYWFVNGRHVRDRLLMSAVRLGYRD
                     VLYHGRHPAYLLQLNLDPRLVDVNAHPAKLEVRFRDSRQVHDFVFRAVERTLAASRPG
                     PDSAAQGATAAPSALAPIASPAGPAAHGYGHAHGRGGAMQSLPFTEGALPQDSPWAVA
                     DIVASRAIEAAPLPADEPLGTALAQLHGVYILAQNAAGLILVDMHAAHERVLYEQYKR
                     QTGTQGAAQTLLDPVVVELKDHEVDAVLEARAEWESLGFEIERLAPERLAVRRVPALL
                     GRRTDLAALLRDVARDALDGGGSRHFEEPGFRLLGTLACRSAIHAHRRLTLPEMNALL
                     RQMEATERADQCNHGRPTWTRLSLAELDQLFLRGR"
     gene            7747..>8618
                     /gene="miaA"
                     /locus_tag="PKN91_09745"
     CDS             7747..>8618
                     /gene="miaA"
                     /locus_tag="PKN91_09745"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNS28396.1"
                     /translation="MAGGAPAPVLVLTGPTGIGKSDWALRLAEELPVEIVSCDSAQVY
                     RGMDIGTAKPDAATRARVPHHLLDLRDPAQSYSAGEFVQDATAALRGIHARGRLPLVV
                     GGTMLYLRALLQGMAALPPAHAAIRDEIDARARSMGWPALHAELARVDPAAAARIHAN
                     DRQRIQRALEVYHATGTAISAWQAAAEAGPREFEFHATALVPADRALLHARIARRFEA
                     MMAAGLLDEVRSLYARPDLTARHPAIRAVGYRQLWHHLAGDYPLDEAVQRAIAATRQL
                     AKRQLTWLKSSFSF"
BASE COUNT         1259 a         2995 c         3065 g         1299 t
ORIGIN      
        1 tggtgctggt cggcgactcg ctcggcatgg tgatccaggg gcacgacacc acggtgccgg
       61 tcacgatgga cgacatcgtc tatcacagcc gcaccgtcgc gcgcggcatc caccgtccgc
      121 tgttgatggc cgacctgccg ttcatgagct atccgtccaa ggagcgtgcg ctcgagaact
      181 cggtgcgcct gatgcaggag ggcggcgccg aaatggtgaa gctcgagagc ggcgccggac
      241 agatcgagat cgtcgaattc ctggccaacc acgacatcgc ggtctgcgcg cacctcggcc
      301 tgaagccgca gtcggtgcac aagacgggcg gctttcgcgt tcagggtcgg ggcgccgagg
      361 cggcgaaacg catgatcgag aacgccaagg cactcgaatc cgcgggcgcc gatatcgtgc
      421 tgctcgagtg cgttcctagc gagctcggcg cggagatcac cgcggcgctc gaggtgccgg
      481 tgatcggcat cggcgcgggg ccgggtaccg acggccagat cctggtgctg tacgacatcc
      541 tcgacatcac cagtgggcgc aagccgcgct tcgtcaagaa cttcatggaa ggcgctgcga
      601 gcaatctcga ggcggtgcgc gcctacgtgc gcgccgtcaa ggactcgagc tatcccgggc
      661 cggaacacgg gttcgcctga gtacgccgat gcaggtgctg acgactatcg ccgacgtgcg
      721 cgcgtgcgtg cgcgcctacc ggcaggcagg cgagcgcatc gccttcgtgc cgaccatggg
      781 caacctgcac gccggacaca tcagcctgat ggagctggcg cgcaccggcc atgaccgctt
      841 catcgcgagc atcttcgtca acccgatgca gttcggcccg aacgaggatt tcgcccacta
      901 tccgcgcacg cccgatcgag atgcgcagat gctgcgggac gcgggctgcg atctgatgtt
      961 catgccggac gtcgccgaga tgtacggcga gggcacgacc gcgccgaccc gcatcgacgt
     1021 gcccgaactc acgacgacgc tgtgcggcgc gttccgcccg gggcatttcg agggcgtcgc
     1081 gaccgtcgtg gcgaagctgt tcaatatcgt gctgcccgac acggccgtgt tcggcgagaa
     1141 ggactttcag cagctcgccg tgatccgccg catggtggcc gacctgtgct tcccggtcac
     1201 ggtcgtcggc gcgccgaccg tgcgcgaagc cgacggcctt gcgatgagct cgcgcaatca
     1261 gtacctgacc gaggccgaac ggcgcgttgc gccgcagatc taccgcgcac tgctggcggc
     1321 caccgcggcg atccgcgccg gcgcggagga ggtcgcgacg atcgaaagcg cgggcgcggc
     1381 gagcctcgcc gcgcacggca tgcaggtgga ctacttcgcg gtgcgccggc gcgtcgacct
     1441 gaccccgccg caggcggctg atcgcgaact cgtcgtgctc accgccgcac gtctcggcaa
     1501 ggcgcgcctc atcgacaatc tgcaggtcga ccggccgtga cgcccgggtg cgtggcggcc
     1561 cgctcgcggt gcctggccaa tgcccatgcc acgtgctcgc gcacgagccc tgacgggtcg
     1621 tccgcgcggc tcgcgagcgc agccagtacc tccgtcgtgg tcggcgcgtt gcctagcgct
     1681 accgcgatgt tgcgcagcca gcgctcgtag ccgatgcggt agatcgccga gccgcgcatg
     1741 cgcgcatcga agtccgcggc gctccaggcg aacagctcgg tgagccgcgg tgtgtcgagc
     1801 gcgtggcgcg cgcgaaagtc ggggtggctc gcctcgcgcg cgaacttgtt ccacgggcac
     1861 acgagctggc agtcgtcaca gccgtagatg cgattgccga gcagcgggcg cagctcctcc
     1921 ggaatgggac cgtgcagttc gatcgtcagg taggaaatgc agcggcgcgc atcgagctgc
     1981 cagggcgcga cgatcgcgcc ggtcgggcag gccgggatgc acgccgtgca ggtgccgcag
     2041 tgcgcgctcg cgggcgcatc gatcggcagt tcgagagtcg tgtagatttc gccgaggaag
     2101 aaccacgagc ccgcatcgcg ggcgatcagg ttggtgtgct tgccgatcca gccgaggccc
     2161 gcgttgcgcg cgagcgcctt ctcgagcacc ggggcgctgt cgacgaagac gcggtgcccg
     2221 aacgggccga cccactcctc gagttgcgcc gcaagcttcg ccagcgcctt gcgcatcagc
     2281 ttgtggtaat cgcgcccgag cgcatagcgg gacacgtagg cgctcgtcgg atcggccagg
     2341 acctgctgcg catcgcggcc gcctggcggc cagtagtcca tgcgcgccga aatcacccgc
     2401 agcgtgcccg gcagcagctc cgcgggtcgg ctgcgcttcg tgccgtggcg ggccatgtag
     2461 tccatctcgc cgtggcggcc cgccgcgagc catcgatcga ggtgtcgctc gtcctcggtg
     2521 agatcgatgc cggtgatgcc tagcgccgcg aagcctagcg cagtggcctc cgcgagcagt
     2581 tggaggcgga gcgtgtcgac atcggctttg tgcatctcga tcatgtcggg cgaccgcggc
     2641 cagtataatc acgcgatggg ccaacctacg gcgatctaca gcgcggcaca ggtccgcgag
     2701 ctcgacgact acgcaatcca gacgctgggc atcccgggct ataccctgat gaagcgcgcc
     2761 ggcgaggcgg gactgcgcgc actgcgtgcg cgctggccga cggcgcatcg catcggcgtc
     2821 gtctgcggcg gcggcaacaa cggtggcgac ggctatgtga tcgcgcgttt cgcgcgcgcc
     2881 gccgggctca ccgtcaccgt gctcgctgcc gtgccggtcg agcagctgcg cggcgacgcg
     2941 cagcgcgccg ccgaagattt cctcgcgagc ggcgggcagg tgcaggagtt cggcgccgcg
     3001 gcgctcaggc aatgcgaagt gatcgtcgat gccctgctcg gaatcggcgc gaagggcagc
     3061 ctgcgccccg aagtgatcgc gatcatcgag gcgatcaata cctcggggcg accggtcttc
     3121 gcggtcgaca ttccgtcggg gctgtgtgcc gactccggca tgccgctcgg cgccgcggtg
     3181 cgcgccgagg tcacgatcac ctacgtcggg ctgaagtgcg gcctcttcat gggcgagggc
     3241 ccggagtaca cgggttcgct gctgttcgat gacctcgagg tcacggcacc tccggcgccg
     3301 caattcgcgc cgcgcctgct tcgcgcgagc gaggcggaca tcgcgcgcgc gttgccgcgg
     3361 cgccgacgag cctcgcacaa gggcagcttc ggccacgtgc tcgtcgtggg tggtggaccc
     3421 ggaatgccgg gcgcggttcg tctcgccgga gaatccgcgc tgcgcgtcgg tgccggcctc
     3481 gtgaccgtcg ccgtggcgcc ggagaatgtc gcgtcgatcg cggcgggctg tcccgaactg
     3541 atcgtcgtgg gtctcgatgt cgcacaggta ggtccgctgc tcgcgagcgc cgatgtcgtc
     3601 gccatcggtc ccggcctcgg gcgcagcgcc tgggcgtggg aagtgctggg cgccacggtg
     3661 gcagccggca agccgctcgt cgtcgatgcc gatgcgctca atatcctggc cgaagatcgc
     3721 acgctgcgcg ccgatcgctg gatcctcacg ccgcaccccg gcgaggcggg gcggctgctc
     3781 ggcacgagta ccgcggccgt gcaggccgac cggctggggt cgctcgagca gctgtgccgg
     3841 acctacggcg gcacggtcgt gctcaagggc gcgggcaccc tggtcggacg cacaggcgat
     3901 acgcctcagc tgtgcgagcg cggcaacccg ggcatggcga cggccggcat gggcgacgtc
     3961 ctcactggcg tgatcgccgg catgctcgcg cagctcggcg acccgctgct ggcggcgcgc
     4021 atcggtgtac tggtgcatgc gctggcgggt gacgcggccg cgcgcggcgg cgaacgcggg
     4081 ctgatcgcgc gcgatgtgat caacgaactg cgcacctggg tcaacctctg agttccgtcg
     4141 acctcgaatt cgcggcgccg tcggcggagg cgatgatggc attcggcgcg gcgctcgcgc
     4201 gtacgcggcc cgaagcaaaa tccgggccgc tgctgctgta cctcacgggc gaactcggcg
     4261 ccggcaagac gacactcgcg cgcggcctgc tcgcacagct tggcgtcacc ggtgccgtgc
     4321 gcagtccgag ctatgcgctc gtcgaagtgc accgcctggc cgaccttacc gtcgtgcacg
     4381 ccgatctcta tcgactgctc gatgccgaag aactcgagcc gctcggtctg cgcgagcttt
     4441 acgcgccgga ccacctttgg gtgatcgagt ggcccgagcg cgccgcgacg gcgctgcccg
     4501 agcccgacct tcacctcgag ctttcgatca cgcctgcggc gcatcgcgtg cggcccgtgg
     4561 cctgtggtcc gcaggggcgg aactggtatg ctgccgccgt cgagcttgtt cgcgtatctc
     4621 attaatttcc attgaattgt gcgcggagcg gctttacatt aagccgccat gcgcagtctc
     4681 gtgtccgcca cactgctgtt gtttgccgcg gcccttgtga cggatgacgc ccggggcgcg
     4741 ggtcgacgtg tcgacggtgt gcgtgcctcg gccgacggtg ccggtgcgcg cgtggtcgtc
     4801 gacctgaccg cgcccgccga cttcaagctg ttcacgctcg agcgcccgcg acgcatcgtt
     4861 ctcgatcttg ccgacacggc actggcggac accgtgaagc tgccgcgcgc cgaggggccg
     4921 atcaccgccg tgcgctccgg ggtgcagccc gggggcacgc tgcggctcgt catcgagacg
     4981 acctcgagcg acgagccgcg cgcctcgcgc attgccccgg ccgcggacgg cggtcaccgg
     5041 ctgctcgtcg agttcggcgc catctcgccg gccgcggcga acacgcgacg cgtcgtggcg
     5101 gcagtacatg cgccgaagca gggcgcgcgc gacgtcgtca tcgcgatcga tgccggacat
     5161 ggcggcaagg atccaggtgc gatcggccac ggcggcacgt tcgagaagac cgtggtgctc
     5221 gagatcgcgc gcgaactcgc caggcgcatc gatgaggcgc ccggcatgcg cgccgtgctg
     5281 atccgcgacg gcgactacta catcgcgcat cgcgagcgca ttcgtcgcgc gcgcaatgcc
     5341 agggccgaca tgttcctgtc gatacacgcc gatgccctgc gcgaccgcgg cgtggcgggc
     5401 gcatcggtgt atgtgctttc cgagcgcggc gcgaccgacg aggccgcacg ctggctcgcc
     5461 gagcgcgaga acgccgccga cctgcggggt ggtgtcacgc tgagcgacaa ggacgatgtg
     5521 atcgcgaccg tgctgctcga cctgtcgcag tcggcggcga tctcggcgag catggaggcg
     5581 gccgagcgga ttctcgcgtc gtttgccggc gtcgtgccgg tacgcaagcc gcgcgtccag
     5641 caggcgggct tcctggtgct gaaatcgccc gatatcccct cgatgctgat cgagaccgcc
     5701 tatatcacca acgcggccga cgagaaacgg ctgcgcagca acgcgcagcg ccgcaagctc
     5761 gcgaattcta tcgtcgacgg cgtccagagc tacttcacgc gcaatccgcc cgagggcacg
     5821 cggttcgccg agctgcgccg cctcgccgcc gcggactgaa cagcgcccgg ttttcggctt
     5881 cggtcattgc cgaggccaat aaccgaaaaa ccggcaaccc ggcaccgtag aatcctccca
     5941 tgccgatttg cgtgcttgcc agtgagctca tcgaccagat cgcggccggc gaggtcatcg
     6001 agcggccggc ttccgcggtc aaggaattgc tggagaacgc gctcgatgcc ggcgcgagcc
     6061 gcatcgagat cgacatcgag cgcggcggcg tcggcctgat ccgggtgcgc gacgacggcg
     6121 cgggcatcgc ggcggccgag ctgccgctcg ccattgcgcg ccacgcgacg agcaagatcg
     6181 cctcgttgga cgatctcgaa tcggtcggca cactcggatt tcgcggcgag gcgctgccct
     6241 cgatcggctc ggtcgcgaga ctgcgcgtga tctcgcgccc tgcaggcgcc gcgcaagcgc
     6301 acgaaattgc cgtcgagggc gggcaggtgg gcgccgtgaa gcccgcggcc catccgccgg
     6361 gtaccacggt cgaagtgcgc gatctgttct acaacgtgcc cgcgcgccgc aagttcgtgc
     6421 gcagcgacgc caccgagacc gggcacatcg cgcgctgggt cgagcggctc gcgctggcgc
     6481 gcccggatgt cgcgttccgg ttgcgcagcg gcacgcgcac gctgctcgat gcgccggcaa
     6541 tcgcggcgga cgaggatccg gcggcgcgcg tcgacgccgt actcggcccg gagttcctcg
     6601 cgcgggcgct gccggtacgg catgcggccg gacccgtcct gatcgaaggc tggatcggcg
     6661 caccgaccgc gtcccgggcg caacccgatt cgcagtactg gttcgtgaac ggccgccacg
     6721 tgcgcgaccg gctgctgatg agcgccgtgc gcctcggcta tcgcgacgtg ctgtaccacg
     6781 gccggcatcc cgcctacctc ctgcagctca acctcgatcc gcggctcgtc gacgtgaacg
     6841 cgcacccggc caagctcgaa gtgcgcttcc gcgacagccg acaggtgcac gacttcgtgt
     6901 tccgcgccgt cgagcgcacg ctcgcggcgt cgcgacccgg tccggacagc gcggcgcagg
     6961 gcgctacggc ggcgccgtcc gcgctggcgc cgatcgcgtc gccagccggt ccggctgcgc
     7021 acgggtacgg gcacgcgcac gggcgcggag gcgccatgca gtccttgccg tttacggaag
     7081 gcgcgctgcc ccaggactcg ccgtgggccg tggcggacat cgttgcgtca cgagcgatcg
     7141 aggcggcgcc gttgccggcg gatgagccgc tcggcaccgc actcgcgcag ctgcacggcg
     7201 tgtacatcct ggcgcagaac gcggccggcc tgatcctggt cgacatgcac gcggcgcacg
     7261 agcgcgtgct ctacgagcag tacaaacggc agacgggcac gcaaggcgcc gcgcagacac
     7321 tgctcgaccc ggtggtcgtg gagctgaagg accacgaggt cgatgccgtg ctcgaagcgc
     7381 gcgcggagtg ggagtcgctc ggcttcgaga tcgaacgcct cgcgcctgaa cgccttgcgg
     7441 tgcggcgcgt gccggcgctg ctcggcaggc gcacggatct cgccgcattg ctgcgcgatg
     7501 tcgcacgcga tgcgctcgat ggaggtggct cgcgccattt cgaggaaccg ggcttccggc
     7561 tgctcggcac gctcgcctgc cgcagcgcca tccatgcgca ccgtcgcctg acgctgccgg
     7621 aaatgaatgc gctgctgcga cagatggaag ccacggagcg ggccgatcag tgcaaccacg
     7681 gtcggccgac ctggacccgg ctgtcgctgg cggaactcga ccagttgttc ctgcggggcc
     7741 gctgaggtgg cgggcggcgc gccggcgccc gttctcgtac tgacgggacc caccggcatc
     7801 ggcaagagcg actgggccct gcgcctcgca gaggagctgc cggtcgagat cgtcagctgc
     7861 gattcggcgc aggtctatcg cggcatggac atcggcacgg cgaagcccga cgcggcgacg
     7921 cgcgcgcgcg taccgcatca cctgctcgac ctgcgcgatc cggcgcagag ctactcggcg
     7981 ggtgaattcg tccaggatgc gaccgccgcg ctgcgcggca tccacgcgcg cggcaggctg
     8041 ccgctcgtcg tcggcggcac gatgctgtac ctgcgcgcgt tgctgcaggg catggcggcc
     8101 ttgccgccgg cgcacgcggc gatccgtgac gagatcgatg cgcgcgcgcg gagcatgggc
     8161 tggcccgcgc tgcacgcgga actcgcgcgc gtcgatccgg ccgccgccgc acgtatccac
     8221 gcgaacgacc gccagcgcat acagcgcgcg ctcgaggtct accacgccac cggcaccgcg
     8281 atcagcgcct ggcaggcggc ggccgaagcg ggcccgcgcg agttcgagtt ccacgcgacg
     8341 gcgctcgtac ccgccgatcg cgcactgctg cacgcgcgca tcgcgcgacg cttcgaggcg
     8401 atgatggccg cgggcctgct cgatgaggtg cggtcactgt acgcacggcc cgatctcacg
     8461 gcgcgccacc ccgcgatccg tgccgtgggc taccggcagc tgtggcacca tctggccggc
     8521 gattacccgc tcgacgaggc cgtgcagcgg gctatcgcgg cgacgcgcca actcgccaaa
     8581 cgccaactga cctggctcaa gtcctcgttt tcattcga
//
LOCUS       DAOQVY010000105         8484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_9269_length_8484_cov_5.049353, whole genome shotgun sequence.
ACCESSION   DAOQVY010000105 DAOQVY010000000
VERSION     DAOQVY010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8484
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9269_length_8484_cov_5.049353"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PKN91_09750"
     CDS             complement(<1..366)
                     /locus_tag="PKN91_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of succinyldiaminopimelate
                     from N-succinyl-2-amino-6-ketopimelate; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HNS28397.1"
                     /translation="MNPRIGALHAYPFQKLAALKAGVVPPAGLAHIAMSIGEPQHAPP
                     ALVLDALRAHLGELGTYPSTIGQPALRAAAARWLERRFALGRDKVDAETMVLPVNGTR
                     EALFAFAQAVTDRAQDPLLV"
     gene            complement(363..3050)
                     /gene="glnD"
                     /locus_tag="PKN91_09755"
     CDS             complement(363..3050)
                     /gene="glnD"
                     /locus_tag="PKN91_09755"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HNS28398.1"
                     /translation="MAATEDESVPTATQRLNWPFLDRLPQRLAADPLSAPLFRDVLRE
                     AQQALAARFHAEEPVESIVRARADLIDVVLRAAWNAHLGDTAQRWSLVAVGGYGRSEL
                     HPYSDVDVLILVPEGLTREERGPLERLLAFLWDIGLEIGHSVRTVAECASESATDVSV
                     MTTLIEARLLAGDGALLAAMREALEPQNVWPVREFFEAKVREQAARHAKANDTAYNLE
                     PNVKTGPGGLRDIQTIAWVARRHFDTATLDELVTHGFLAPSELRKLKQARAFLWRVRC
                     ALHLIAGRREDRLLFDHQVRLAQSFGYEDASYTLAVEQFMQRYYRTVMDVSLLNELLL
                     QLFREAILTAGTPPQPLNPRFQVRNDYLEAISDDLFARTPSALLELFVLLQQHPQIRG
                     VRAETIRAIGRSLWLIDEEFRQNPRHHRLFLEILRAPVGVTHELRRMNNYGVLGRYIP
                     AFGRIVGRMQYDLFHAYTVDAHTLFVVSNLRRLAIERYNHELPQVSQVFAELPKPELA
                     YLAALFHDIAKGRGGDHSELGSVDAEAFCLEQGLSRYDARLVAWLVRNHLILSVTAQK
                     QDISEPEVINAFARRVGDETHLNYLYVLTCADVRGTDPKLWNSWKATLFHEFYERTRR
                     ALRRGLESPIDQDELIRETQEAARAALRAAGITDAQVDAVWSRLSDGYFLRSTPEEVT
                     WHTRLLADRDPRAEDPVVALDAQSLRGTTAIFAFAPLRRHGFARTTAVLDQLGLTIVG
                     ARITPTEDGYSLDLYHVLEDDGAPITDKERLLEIERALWHSLHSPEEHPLAVSRRAPR
                     QSRMFSTPTHILLSTDEHHGRSVLELTAADRPGLLCDVGKVLMEERLHLHNARIMTVG
                     ERAEDVFYITDSHNRPLDSASADRLQQRLTEVLERRRAA"
     gene            complement(3073..3840)
                     /gene="map"
                     /locus_tag="PKN91_09760"
     CDS             complement(3073..3840)
                     /gene="map"
                     /locus_tag="PKN91_09760"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNS28399.1"
                     /translation="MQVTIKTLDEQEKMRVAGRLAADVLDMITAHVQPGTTTEELDRL
                     CHEYIVGVQQSVPANLNYRGFPKTICTSVNHVVCHGIPNEKRLKSGDIVNIDVTVIRD
                     GFHGDTSRMYFVGKPGIQAQRLTQTCFEAMWEGIRQVRPGQRLGDIGHAIQRYVEERN
                     FSVVREFCGHGIGRVYHEDPQVLHYGEPGTGIELRPGMTFTIEPMVNAGKRHVAVLKD
                     GWTVVTKDHSLSAQWEHTVLVTDDGVEVMTLGAADRH"
     gene            4029..4703
                     /gene="rpsB"
                     /locus_tag="PKN91_09765"
                     /pseudo
     CDS             4029..4703
                     /gene="rpsB"
                     /locus_tag="PKN91_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
     gene            5097..6008
                     /gene="tsf"
                     /locus_tag="PKN91_09770"
     CDS             5097..6008
                     /gene="tsf"
                     /locus_tag="PKN91_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNS28400.1"
                     /translation="MATIITADSVKQLRERTGAGMMECKRALVETGGDLDAAAELMRK
                     QGLAKADKKATRIAAEGVIAMASAADGLAAAMVEVNCETDFVAREQDFRGFADEVAAV
                     AIASQAGSLEALLAAKLPSGATVEERRRALIAKIGENITVRRFTIVRAPAQLGSYLHG
                     TRIGALVALEGGDETLARDLAMHVAASNPQYLAAADVPADIIARERDILAEQAKGDPK
                     NQGKPDNILAQIVEGKVRKALAEITLLGQPFVRDDKQSVEKLLAGAKAKVVAFERFEV
                     GAGIEKKQEDFAAEVMAQVKGAAGGAH"
     gene            6074..6805
                     /gene="pyrH"
                     /locus_tag="PKN91_09775"
     CDS             6074..6805
                     /gene="pyrH"
                     /locus_tag="PKN91_09775"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004751913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNS28401.1"
                     /translation="MTKPGDSPRYRRILVKLSGEALLGAADYGIDPVMLKRIAAEIRD
                     IAALGVEVAVVIGGGNIFRGAGLARAGMDRVTADQMGMLATVMNALAMQDALEGLGIY
                     ARVMSAIRINEVCEDYIRRRAVRHLEKGRIVIFAAGTGNPFFTTDTAASLRAIEIEAD
                     LLLKATKVGGIYSDDPVKNPAAKHHARLTYDQVLDGRLNVMDQTAIVMCRDNDLPLRV
                     FNLNNAGDLLRIVQGEDVGTIVVKD"
     gene            6822..7379
                     /gene="frr"
                     /locus_tag="PKN91_09780"
     CDS             6822..7379
                     /gene="frr"
                     /locus_tag="PKN91_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNS28402.1"
                     /translation="MLEDIKKDAAARMAKCVQTFQGDLRKLRTGRAHPSLVEHLKVDY
                     YGSDVPLSQVANITIEDARTITITPWEKTMVQVIEKAIHKSDLGLTPMSAGAVIRVPL
                     PPLTEERRRDITKVVRQDAENARVAVRNVRRDVMNDTRELQKEKMITQDDEKRAEAEI
                     QKLTDKYIAEIDQQLAAKEKEIMQV"
     gene            7399..8160
                     /gene="uppS"
                     /locus_tag="PKN91_09785"
     CDS             7399..8160
                     /gene="uppS"
                     /locus_tag="PKN91_09785"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNS28403.1"
                     /translation="MGATDTQIPAGAVLPRHVAIIMDGNGRWAEQRGLARHAGHKHGI
                     PVVRMCVEECVRRGIGALTLFAFSSENWRRPESEVTQLMSLFVESIERELPDLHANGV
                     RLRVIGDRHTLGVRLQSRVAEAEHHTRANTRLALQVALSYGGRWDIVQAARSVAQAVA
                     AGALRTADIDEARFAAGLALADLPDPDLFIRTGGEHRISNFLLWNLAYTELWFTETLW
                     PDFDVGAFDRALACFAARDRRFGLTRAQREAAVRA"
     gene            8153..>8484
                     /locus_tag="PKN91_09790"
     CDS             8153..>8484
                     /locus_tag="PKN91_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28404.1"
                     /translation="MLKERIRTALILGALLIVVLFALPVWAAVAVITLALLAGAWEWS
                     ALIGRSGQVGIASRLLFVVAIAAGMAALALPEPALLPESTVLAIAVLWWLAGFTWVKD
                     SVAVAADIG"
BASE COUNT         1404 a         2742 c         2986 g         1352 t
ORIGIN      
        1 gacgagcagc ggatcttgcg cccggtccgt gaccgcttgc gcgaacgcga acagtgcctc
       61 gcgcgtgccg ttcaccggca gcaccatcgt ctccgcgtcg accttgtcgc gcccgagcgc
      121 gaagcggcgc tcgagccagc gcgccgcggc tgcgcgcagt gccggttggc cgatcgtcga
      181 cggatacgtg ccgagttcgc cgaggtgggc gcgcaaggca tcgagcacca atgcgggtgg
      241 ggcatgttgc ggctcgccga tcgacatcgc gatatgggcg aggccggccg gcggtaccac
      301 gcccgccttc agcgccgcca gcttctggaa cgggtaggcg tggagcgcgc cgatgcgcgg
      361 gttcatgccg cgcgccgccg ctcgagcact tcggtgaggc gctgctgcag ccggtccgcg
      421 gatgctgaat cgagtggccg gttgtgcgag tcggtgatgt agaagacgtc ctcggcgcgc
      481 tcacccacgg tcatgatccg ggcgttgtgc aggtgcagac gctcctccat cagcaccttg
      541 ccgacatcgc acagcaggcc gggacggtcg gcggccgtga gttcgagcac cgagcgcccg
      601 tggtgttcgt cggtgctgag caggatgtgg gtcggtgtgc tgaacatgcg tgactggcgc
      661 ggcgcacggc gggacaccgc gagcgggtgc tcttcgggcg agtgcagcga gtgccacagc
      721 gctcgctcga tctcgagcag gcgctccttg tcggtgatcg gcgcaccgtc gtcttcgagc
      781 acgtggtaca ggtcgaggct gtagccgtcc tcggtcggcg tgatgcgcgc gccgacgatc
      841 gtgagcccga gctggtcgag caccgcggtg gtgcgcgcga agccgtggcg acgcagcggc
      901 gcaaacgcga agatcgcggt cgtgccacgc aggctctgcg catcgagcgc caccaccgga
      961 tcttcggcgc gcggatcgcg atccgcgagc agccgggtat gccaggtgac ttcctcggga
     1021 gtgctcctca ggaaataacc gtcgctgagg cgcgaccaga ccgcgtcgac ctgcgcatcc
     1081 gtgatgccgg ccgcgcgcag cgccgcgcgc gcggcctcct gcgtttcgcg gatcagctcg
     1141 tcctggtcga tcgggctctc gagcccgcgg cgcagcgcac gccgcgtgcg ttcgtaaaac
     1201 tcgtggaaca gcgtggcctt ccaggagttc cacaatttcg ggtcggtgcc gcgcacatcg
     1261 gcgcaggtca gcacgtagag ataattgagg tgggtttcgt caccgacccg ccgcgcgaac
     1321 gcgttgatca cctccggttc ggagatgtcc tgcttctggg ccgtgaccga caggatcagg
     1381 tggttgcgca ccagccaggc gacgagtcgc gcgtcgtagc gtgacaggcc ctgctcgagg
     1441 cagaaggctt cggcgtccac ggagccgagc tccgagtgat cgccgccgcg ccccttggcg
     1501 atgtcgtgga acagggcggc aagataggcg agctccggct tcggcagctc ggcaaacacc
     1561 tgtgacacct gcggcagttc gtggttgtag cgctcgatcg cgaggcgtcg caggttgctg
     1621 acgacgaaca gcgtgtgcgc atcgaccgtg taggcgtgga acagatcgta ctgcatgcgc
     1681 ccgacgatgc ggccgaatgc cgggatatag cggccgagca cgccgtaatt gttcatgcgg
     1741 cgcagttcgt gcgtgacgcc gaccggcgcg cgcaggatct cgaggaacag gcggtggtgg
     1801 cgcgggttct ggcggaattc ctcgtcgatc agccacagcg aacggccgat cgcgcggatc
     1861 gtttcggcgc gcacgccgcg gatctgcggg tgctgctgca gcagcacgaa cagttcgagc
     1921 agcgccgacg gcgtgcgggc gaacaggtcg tcgctgatcg cctcgaggta gtcgttgcgc
     1981 acctggaagc gcggattgag cggttgcggc ggcgtgcccg cggtcaggat ggcctcgcgg
     2041 aacagctgca gcagcagctc gttcagcagg ctcacatcca tcacggtgcg gtagtaacgc
     2101 tgcatgaact gctcgaccgc gagcgtgtag gaggcatcct cgtagccgaa gctctgcgcg
     2161 agcctgacct ggtgatcgaa cagcaggcga tcctcgcggc gcccggcaat caggtgcagg
     2221 gcacagcgca cgcgccacag gaaggcccgg gcctgcttga gcttgcgcag ctccgacggc
     2281 gcgaggaagc cgtgagtgac gagttcgtcc agcgtcgcgg tgtcgaagtg gcgcctggcc
     2341 acccacgcga tcgtctggat gtcgcgcagt ccgcccgggc cggtcttgac gttcggttcc
     2401 aggttgtaag cggtatcgtt ggccttcgcg tgccgcgcgg cctgttcgcg cactttcgcc
     2461 tcgaagaatt cgcgcaccgg ccacacgttc tgcggctcga gcgcttcgcg catcgcggcg
     2521 aggagcgcgc cgtcgcccgc gagcaggcgc gcttcgatca acgtggtcat taccgacacg
     2581 tcggtggcgc tttcgctcgc gcactccgcg acggtgcgca cgctgtgtcc gatctcgagc
     2641 ccgatgtccc acaggaaagc gagcagccgc tccagcgggc cgcgctcctc ccgggtcagg
     2701 ccttccggca cgaggatcag cacgtcgacg tccgagtacg ggtgcagttc gctgcggccg
     2761 tagccgccca cggccaccag cgaccagcgc tgtgccgtgt cgccgaggtg ggcattccag
     2821 gcggcacgca gcaccacatc gatcaggtcc gcgcgcgcgc gcacgatcga ttcgaccggc
     2881 tcctcggcgt ggaagcgggc ggcgagcgcc tgctgcgcct cgcgcagcac gtcgcgaaac
     2941 agcggcgcgg acagcgggtc cgcggccagg cgctgcggca ggcgatcgag gaacggccag
     3001 ttcagccgtt gcgtcgcagt gggaacactc tcgtcttcgg tcgcggccac gtgcggttcg
     3061 ctgcggccgg cgtcagtgcc gatccgcggc gccgagggtc atcacctcga cgccatcgtc
     3121 ggtcacgagc accgtgtgct cccattgtgc cgacagtgaa tggtccttcg tcacgacggt
     3181 ccagccgtcc ttgagcacgg cgacatggcg cttgccggcg ttgaccatcg gctcgatcgt
     3241 aaaggtcatg ccggggcgca gctcgatgcc ggtgcccggc tcgccgtagt gcagcacctg
     3301 gggatcctcg tggtagaccc ggccgatgcc gtggccgcag aactcgcgca ccaccgagaa
     3361 gttgcgttcc tcgacatagc gctggatcgc atgcccgatg tcgccgaggc gctggccggg
     3421 ccgtacctgg cggatgcctt cccacatcgc ctcgaagcag gtctgggtga ggcgttgcgc
     3481 ctggatgccc ggcttgccga cgaaatacat gcggctcgtg tcgccgtgga atccgtcccg
     3541 gatcacggtg acgtcgatgt tgacgatgtc gcccgatttc aggcgcttct cgttcggtat
     3601 gccgtggcac accacgtggt tgaccgaggt gcagatcgtc ttcgggaagc cgcggtagtt
     3661 gagattggcc ggtacggact gctgcacgcc gacgatgtat tcgtggcaca gccggtccag
     3721 ttcctcggta gtcgtgccgg gctggacgtg tgcggtgatc atgtcgagga catcggccgc
     3781 caggcggccg gccacacgca tcttctcctg ttcgtccagg gtcttaatcg ttacttgcat
     3841 caggggcggg cgggaggcgt aaggcgttgt tttcgcgggg ggcacatggt ataaagcgcg
     3901 gccttttttg gcaacgcaat ccacacgcgt caccagttca cttccggttg ggtgtttccg
     3961 ctcacggctt gggccaggcg gggatggccg gaagggcggc gcggaggctc aacccgacag
     4021 gagtttttat gtccagcgtg tccatgagac agatgctgga agcgggcgtc catttcggac
     4081 accagacccg cttctggaac ccgaagatgg cgccgttcat tttcggtgaa cgcaaccgca
     4141 tccacatcat caacctcgag aagacgcagc cgctctacca ggaggccgcg ggcttcatca
     4201 agaacgtcgt cgcggacggc ggcacggtgc tgttcgtcgg caccaagcgt tccgcgcgcg
     4261 aggccgtgca ggccgaggcc gtgcgttgcg gcatgcccta cgtcaaccag cgttggctcg
     4321 gcggcatgct cacgaatttc aagaccatcc gccagtcgat caagcggctc gccgacctca
     4381 ccgagatgcg cgagagcggt gtgctcgaca agcgcggcaa gaaggaagcg acccagttgc
     4441 gccgcgaaat ggaaaagctc gagcgcagcc tgggcggcat caaggacatg gagtcgctgc
     4501 ctgatgcact gttcgtgatc gacgtcggcc acgagaacat cgcgatccac gaggcgaaga
     4561 agctcggcat cccggtcgtc gcgatcgtcg acaccaattg ctcgccggac ggcatcgatt
     4621 tcgtcgtccc gggcaatgat gacgcgatgc gcgccatcct gctgtacgcg agcggggtcg
     4681 ccgaagccgt gctcgagggc aaggcatcgg tgccgcaggt ggcggtgggc gaggacgagt
     4741 tcgttgaact cgatgcggaa ggcaatccca agaagagacc gggccgtggg cgccgcccgt
     4801 cggccgctcc ggtgcgcagc cgcaaggcgg ccccggtgcg ccgtcgtccg gccgccgcgc
     4861 cgaccgccgc caaggctgac gagacaccgg tggagggtgc gcccgacgct gccgcgaccg
     4921 aggctgccgc ggtgccggcc gccgatcccg cgatcgatgc ggcgatcgcc gaggccgatg
     4981 cgctcgagga cgtggtcgtg accgacgacg acggcgaacg cagtgtcagc gggtcggcga
     5041 cgcgccgtcg tgcgccgact ggcggagcgc gccgtcccgg cgcgaagggc tgagccatgg
     5101 ccacgatcat cactgcagat tccgtcaagc aactgcgcga gcgcacgggc gccggcatga
     5161 tggagtgcaa gcgcgcgctc gtggaaaccg gcggcgacct cgacgctgcc gccgaactga
     5221 tgcgcaagca gggtcttgcg aaggccgaca agaaggcgac ccgcatcgcc gccgaaggcg
     5281 tgatcgccat ggcgagcgcg gccgacggcc tcgctgccgc catggtcgaa gtgaactgcg
     5341 agaccgactt cgtcgcgcgt gagcaggact tccgcggttt cgcggatgag gtggcggccg
     5401 tcgcgatcgc atcgcaggcc ggctcgctcg aggcattgct cgcggcgaaa ctgccgtccg
     5461 gtgcgacggt cgaggagcgg cgccgcgcgc tgatcgcgaa gatcggcgag aacatcacgg
     5521 tgcgccggtt cacgatcgtc agggcaccgg cccagctcgg tagctatctg cacggcacgc
     5581 gtatcggagc gctggtcgcg ctcgagggcg gggatgagac cctcgcccgg gatctcgcga
     5641 tgcacgttgc agccagcaat ccgcagtacc tcgcggccgc ggacgtgccc gccgacatca
     5701 tcgccaggga gcgcgacatc ctcgcggagc aggcgaaagg cgatccgaag aaccagggca
     5761 agcctgacaa catcctcgcg cagatcgtcg agggcaaggt ccgcaaggcc ctggcggaga
     5821 tcacgctgct cgggcagccg ttcgtgcgcg acgacaagca gagcgtagag aagctgctgg
     5881 cgggcgcgaa ggcgaaagtc gtcgcgttcg agcgcttcga ggtcggcgcc ggcatcgaga
     5941 agaaacagga agacttcgct gccgaggtca tggcccaggt aaagggcgcg gcaggcgggg
     6001 cgcactgaac aggaacggca aagtcaggga aaccccgccg caagcggggt ttctttcatg
     6061 agggcatagg cacgtgacga aacccggcga ctcaccgcgc tacaggcgca tcctggtcaa
     6121 gctctcgggc gaagctttgc tgggggccgc ggactacggc atcgatcccg tgatgctgaa
     6181 gcgcatcgcg gccgagatcc gcgacatcgc cgcgctcggt gtcgaggtgg ccgtggtgat
     6241 cggcggaggc aacatcttcc gcggcgcggg tcttgcgcgt gccggcatgg atcgcgtgac
     6301 ggccgaccag atgggcatgc tggccaccgt gatgaacgcg ctcgcgatgc aggacgccct
     6361 cgaggggctc gggatctatg cgcgcgtgat gagcgcgatc cgcatcaacg aggtgtgcga
     6421 ggactacatc cgccgccgcg ccgtgcgcca cctcgagaaa gggcgcatcg tgatcttcgc
     6481 ggccggcacc ggcaatccgt tcttcacgac cgacaccgcc gcatcgctgc gcgcgatcga
     6541 gatcgaggct gacctgctgc tgaaggccac caaggtcggc ggcatctatt ccgacgatcc
     6601 ggtgaagaat ccggcggcaa agcatcatgc gcgcctgacc tacgaccagg tactcgacgg
     6661 gcgtctgaac gtcatggacc agaccgcgat cgtgatgtgc cgcgacaacg acttgccttt
     6721 gcgggtgttc aatctcaaca atgcggggga cctgctgcgc atcgtgcagg gcgaggacgt
     6781 cggcacgatc gtggtgaagg actgatacgg ggatcgcaga catgctcgag gacatcaaga
     6841 aagacgccgc ggcccgcatg gccaagtgcg tgcagacctt ccagggcgac ctgcgcaagc
     6901 tgcgcaccgg gcgcgcgcac ccgagcctgg tcgagcacct gaaggtcgac tactacggct
     6961 ccgacgtgcc gctctcgcag gtcgcgaaca tcacgatcga ggacgcacgc acgatcacga
     7021 ttaccccgtg ggagaagacc atggttcagg tgatcgagaa ggcgatccac aagtccgacc
     7081 tcgggctcac gccgatgagc gcgggcgcgg tgatccgcgt gccgctgccg cctctgaccg
     7141 aggagcgccg ccgtgacatc accaaggtcg tgcggcagga tgccgagaat gcacgcgtcg
     7201 cggtgcgcaa cgtgcgtcgc gacgtgatga acgacacccg ggagctgcag aaggaaaaga
     7261 tgatcacgca ggacgacgag aagcgcgccg aggccgagat ccagaagctc accgacaagt
     7321 acatcgccga gatcgaccag cagctcgccg ccaaggaaaa ggagatcatg caggtctaga
     7381 cctcatggtc atcggcatat gggcgcgacc gacacacaaa ttcccgccgg tgcggtcctg
     7441 ccgcggcacg tggcgatcat catggacggc aacggacgct gggccgagca gcgcggcctc
     7501 gcgcgccatg ccggccacaa gcacggcata ccggtcgtgc gcatgtgcgt cgaggagtgc
     7561 gtgaggcgcg gcatcggcgc gctgacactg ttcgcgttca gcagcgagaa ctggcgccgc
     7621 cccgaatccg aggtgacgca gctgatgagc ctgttcgtcg agtcgatcga gcgcgagctg
     7681 cccgatctgc atgccaatgg cgtgcgtctg cgcgtgatcg gcgatcgcca cacgctcggc
     7741 gttcgcctgc agtcgcgcgt cgccgaggcg gagcaccaca cacgcgccaa tacgcgcctc
     7801 gcgctgcagg tggcgctgag ctatggcggg cgctgggata tcgtgcaggc cgcgcgctcg
     7861 gtggcgcagg cggtggccgc cggtgcgctg cggaccgccg acatcgacga ggcgcggttt
     7921 gccgccggcc tcgcgctcgc cgacctgccc gatccggacc tgttcatccg cacgggcggc
     7981 gagcaccgca tcagcaattt cctgctctgg aacctcgctt acacggagct gtggttcacc
     8041 gagactctat ggcccgactt cgacgtcggc gcgttcgacc gggctctcgc ctgttttgcc
     8101 gcgcgcgacc ggcgcttcgg gctcacgcgc gcgcagcggg aggccgccgt ccgtgcttaa
     8161 ggaacggata cgcacggcgc tgatcctcgg cgcgctgctg atcgtcgtac tgttcgcgct
     8221 gcctgtatgg gcagcggtcg ccgtgatcac gctggcactg ctcgccgggg catgggaatg
     8281 gtcggcgttg atcggcaggt ccggccaggt gggcattgcc tcgcgcctgc tgttcgtggt
     8341 cgcgatcgcc gcgggcatgg ccgcgttggc cttgcccgag cccgcgctcc tgcccgagtc
     8401 gaccgtgctc gccattgcag tcctgtggtg gctcgccgga ttcacctggg tcaaggattc
     8461 cgttgccgtt gccgccgata tcgg
//
LOCUS       DAOQVY010000106         8384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_9416_length_8384_cov_5.610157, whole genome shotgun sequence.
ACCESSION   DAOQVY010000106 DAOQVY010000000
VERSION     DAOQVY010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8384
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9416_length_8384_cov_5.610157"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..717)
                     /gene="msrA"
                     /locus_tag="PKN91_09795"
     CDS             complement(82..717)
                     /gene="msrA"
                     /locus_tag="PKN91_09795"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNS28405.1"
                     /translation="MPLFQKRARMPTAADALPGRDTPLPVPARHYVNGNPLQPPFEAP
                     LEEAVFGMGCFWGAERLFWQLPGVFSTAVGYAGGYTPNPTYEEVCSGATGHAEVVRVI
                     FDPRVVSYATLLKVFWESHDPTQGMRQGNDLGTQYRSVIYAVGQEQLRAAQESRHAYQ
                     ARLDAAGKGRITTDIAEAPRFYYAEDYHQQYLAKNPGGYCGIGGCGIAYSA"
     gene            799..1392
                     /locus_tag="PKN91_09800"
     CDS             799..1392
                     /locus_tag="PKN91_09800"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNS28406.1"
                     /translation="MTRSASTPRLRLTLLYFVALLVATALCAPRHPPGALLQAAALLL
                     VAGACLGRIWCSVFIAGRKDAAIVTHGPYALCRHPLYSLSFVGGIGLALATGSFTLVA
                     ATAIVLAALFGSAARREERWLGERHPGEYTRYAASTPRWWPAFSRWSMPAETLVYPPI
                     LWKAFVDAGSFFLLYALVLAAGALQGSGVLPRLAVLP"
     gene            1389..2039
                     /locus_tag="PKN91_09805"
     CDS             1389..2039
                     /locus_tag="PKN91_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HNS28407.1"
                     /translation="MTELELKRDLFGSVFLVLDDAGRPLHVCRDTRTARAWVMPLARW
                     LARREARALRRLEGVGAVPRLIDHRAGMLSREWLAGEPMQRARPADPHYFRDALTLLR
                     RLHLHRVVHNDLAKEPNWLVTPEGLPALVDFQLAWAARRRGRLFRALAHDDLRHMLKH
                     KRSYLPGRLTARERRILATPSVLSRAWMASGKKVYLFVTRRILNWSDREGAGDRRF"
     gene            2055..2324
                     /locus_tag="PKN91_09810"
     CDS             2055..2324
                     /locus_tag="PKN91_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28408.1"
                     /translation="MGISQPHNLRGNPDSRSQRPYGLRVSLHGNDPFRKLLGADWSRT
                     HWYTTVAERDAALAEMSRRHEYSRPGDKPALRFEKVEKLAESRGL"
     gene            complement(2332..4536)
                     /locus_tag="PKN91_09815"
     CDS             complement(2332..4536)
                     /locus_tag="PKN91_09815"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNS28409.1"
                     /translation="MRVPRLIAWLCVVMPIFAAAADTRPRVGLVLSGGGARGAAHVGV
                     LKVLEEMQVPIDAIAGTSMGAVVGGLYASGLSATEIDAALHGLDWGDVLGDRPPRRDL
                     SFRRREEDRNFLVRLPLGLRGGEFRLPKGLVQGQRLHDLFRKLTLPAATIGDFDALPT
                     PFRALATDLETGERIVLDSGDLATAMRASMSVPGVFVPVERDGRLLVDGGLSDNLPVD
                     VARAMGVDVLIVVDTGFPLLTRDKLSTVPIISNQMLAILIRRNSELQRASVTDGDILI
                     DPALANASSFDFERFADSIQAGADATRAAASALARLAVSQPRYQAYVDARAQVRAREI
                     PQIGFLRVAEGSQRYQRPIEDLFEEFVGAPLDGEAIDARVTELYGLGNLETLDYQIVS
                     DEAGREGLEVTATRNSWGPNYVRFGLSLGDDFEGNSAYNASARFVLAEINQLGGEWVW
                     DLQAGESPRVATEVYLPLSYRNRWFVLPQGEYGARNVGLFDTDGRRLAEYRIKGARYG
                     LDFGREFGGAFEVRVGVHREDGESRVRVGDPALPVYEFDVRQLYARIAYDSLDDVGFP
                     RTGTSLFIDWTGERDLSGTSSDADLVSIDWQIARSWGRNSAVFWASAGSNLDSAPGDV
                     RNLFPLGGFLNLSGLAPGEIAGRHFAIARAIYYRQIGRPGTAFLDLPTYLGMAWEVGN
                     VWEERSDIAFDSARNDFSVFLGVRSLLGPLYFGLGYDDGGEMAYYLSLGRLF"
     gene            4579..6522
                     /locus_tag="PKN91_09820"
     CDS             4579..6522
                     /locus_tag="PKN91_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HNS28410.1"
                     /translation="MLRSSLLLLSLAAAVAHGQSPIVSDWGHYGGDVFAQRYSSLNEI
                     DRSNVDKLEVAWTYRTGELGAGFERAGKLSFEATPVLAFGLLYLSTPTNIVIALDPAT
                     GTERWRHDPQIPRNQHYSEGTSRGVTVWEDPGEARQGACRRRVFTGTLDARLIALDAE
                     SGEPCKDFGPGGAVALAPDARPTSPGEFLVTSPPATFDDVVIVGSAIGDNRGVELERG
                     IVRGFDARSGALLWRFDPVPDSETHPAWGDWRPEQARQTGGGNAWAPITVDPGRGLAF
                     IPTGSASPDFYGGQRLGNNAMANSLVALDARTGEVRWFRQLIHHDLWDFDLAAQPTLI
                     EIEGETRSVAAVVQATKSGLVFVLDRETGEPVYGIEERKVPASRVPGEVASPTQPFPS
                     TPPLVSHAPLTEDDAWGLTFWDRGKCRKLIAKYRNEGAYTPPDLEGTILYPSYVGGVN
                     WGGVAHDVRRNHIITVVNHIPMVVTLAPRELLRRQRESGDYPHSEFSAQRGTPYGMRR
                     EPLFSPFKLPCVRPPWGTLVSIDIERNAIAWQVPLGSTRGVAPAWLPARDFGMPSMGG
                     PIVTAGDLVFVAAAMDNQIRAFDIETGKELWKRALPAGGQATPMTYRAGPDRRQYVVI
                     AAGGHGGLGTPKGDYVIAYALPK"
     gene            6519..>8384
                     /locus_tag="PKN91_09825"
     CDS             6519..>8384
                     /locus_tag="PKN91_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS28411.1"
                     /translation="MRRGAFAFLLATAGAHAADATLQEFVVTTTRLPAPRIDVPASVG
                     LVDAGDITRVGATHYSEAVNRIPGVYVQRGGGQESLVSVRSPVLTGAGACGAFLAAED
                     GLAIRPVGFCNVNDLFEVNTEQAGGIEVLRGPGSAIHGASAVHGIINILTPSVAELPR
                     LGLALEGGQDSYRRVKVATRTPGGTRGFGTYGHYTHDGGFRDDSGVDEGKLNLLYDEA
                     DVAGGTLRVRAAGTWLEQESAGYIRGYESYKDEAIVQTNADPEAFRDAWSARGSAAWS
                     RSTCEGCAADVRLIVRRSQMQFLQHFLIGKPLEENAQSSAMVSASTRRPLGAAWSWGA
                     GLDLEASDSDLRETQNGPATDGTPAANAIRPAGKHYDYGVDGRTAGAFATLGWRPDPR
                     WVLNAGLRIERTRYDYDNRMIAGNTDENGVPCGAAGCLYSRPADRVDEFDNVAPKIDV
                     MYLPRERHRLYLSWSRGFRPPEQTELYRLQRQQDIADLDSERLQGGEIGWRAAMGDFD
                     WSLAAFALDKRNVIVRDSSGFNVNGGETTHRGIEYELRWRLSPYWRASAAGSVAWHRY
                     AFSAAVEGGETITDGNDVDTAPRNVHALRIGWSTATNLDAEFEVLHVGRYWLDASN"
BASE COUNT         1309 a         2985 c         2844 g         1246 t
ORIGIN      
        1 gcacgcgcct gtgcaggccg aaagagcgcg tgttcgagat cctgccgccg atcccggggc
       61 tgtaggcgca cggcagcgcg cctacgcgga ataggcgatg ccgcagccgc cgatgccgca
      121 atacccgcct gggttcttgg cgaggtactg ctggtgatag tcctcggcgt aatagaaccg
      181 cggcgcctcg gcgatatcgg tcgtgatccg gcccttgccg gcggcatcga gtcgcgcctg
      241 gtaggcatgt cgtgattcct gggcagcgcg cagctgctcc tgccccaccg cgtagatcac
      301 cgatcgatac tgcgtgccga gatcgttgcc ctggcgcatg ccctgcgtgg ggtcgtggga
      361 ctcccagaac accttcagca gcgtggcata gctgacaacg cgcggatcga aaatgacccg
      421 cacgacttcg gcgtgcccgg tggcacccga gcacacctcc tcgtaggttg gattcggcgt
      481 gtagccgccg gcatagccca cagcggtcga gaagacgccc ggcaactgcc agaaaagccg
      541 ctcagccccc cagaaacagc ccatgccgaa cacggcttct tcgagcggcg cctcgaacgg
      601 cggttgcagg ggattgccgt tgacgtagtg cctggcgggt accggcagcg gcgtgtcgcg
      661 gcccggcaag gcatcggccg cggtcggcat gcgcgccctc ttctggaaca gtggcatgag
      721 gatcgcctcc gtgaagggtg gccagtgtgg gcgcgcgcgg cccccgactc aaggtcatgt
      781 gccacaatcg ccgcgcccat gacccgctcc gccagcactc cgcgcctgcg cctcacgctc
      841 ctctacttcg tcgcgctgct cgtggcgact gcattgtgcg ccccgcgaca cccgcccggt
      901 gcgctgctgc aggccgcggc gctcctgctg gtcgccggcg cttgcctcgg gcggatctgg
      961 tgttcggtct tcatcgcggg gcgcaaggac gcggccatcg tgacgcacgg tccctatgcg
     1021 ctctgccgcc acccgctcta ctcgctgtcg ttcgtcggcg gcatcggcct cgcactcgcc
     1081 accggatcgt tcaccctcgt ggcagcgacg gcgatcgtgc tcgccgcgct cttcggcagc
     1141 gccgcgcgac gcgaagagcg ctggctcggc gagcgtcatc cgggcgagta cacgcgttac
     1201 gcggccagca cgccgcgctg gtggccggcg ttctcgcgct ggtcgatgcc ggccgagacg
     1261 ctcgtttatc cgccgatcct gtggaaagcg ttcgtcgatg cgggctcgtt cttcctgctt
     1321 tatgcgctgg tgctcgcggc gggcgcgctg cagggctccg gcgtgctgcc gcggctcgcc
     1381 gtgctgccgt gaccgaactc gagctgaagc gcgacctgtt cggctcggtg ttcctcgtcc
     1441 tggacgatgc cggccggccg ctgcatgtgt gccgcgacac gcgcaccgcg cgcgcgtggg
     1501 tcatgccgct cgcgcgctgg ctggcgcggc gcgaggcgcg cgcgctgcgg cgcctggagg
     1561 gcgtcggcgc cgtaccgcgc ctgatcgacc atcgcgccgg catgctcagt cgcgaatggc
     1621 tggccggcga acccatgcag cgcgcgcgcc cggcggaccc gcactatttc cgggacgccc
     1681 tcacgctgct gcgccgcctg cacctgcacc gcgtcgtgca caacgatctc gccaaggaac
     1741 cgaattggct cgtgaccccg gagggcctcc cggcgctcgt cgatttccag ctcgcctggg
     1801 ccgctcgccg gcgcggccgg ctgttccggg cgctggcgca cgacgacctg cgacacatgc
     1861 tgaaacacaa gcgcagctac ctgccgggac ggctcacggc gcgcgagcgg cgcatcctgg
     1921 caacgccctc tgtgctgtcg cgcgcctgga tggcgagcgg caagaaggtc tatctgttcg
     1981 tcacgcgccg tatcctcaac tggtcggacc gcgagggcgc gggcgatcgc cgcttctgat
     2041 aatcttcgcg cccgatgggt atttcacaac cgcacaatct gcgcggcaat cccgacagcc
     2101 gcagccagcg accttacggc ctgcgcgtga gcctccacgg caacgatcca ttccgcaagc
     2161 tgctgggcgc cgactggagc cgcacgcact ggtacacgac ggtcgcggaa cgcgacgccg
     2221 cgctcgccga aatgagccgc aggcacgaat actcgcgccc cggcgacaag ccggcgctgc
     2281 gtttcgagaa ggtcgagaag ctcgccgaaa gccgcgggct gtaggcgccg cctagaacag
     2341 gcggccgagc gagaggtaat aggccatctc gccgccgtcg tcgtagccca ggccgaaata
     2401 cagcggaccg agcaggctgc gcaccccgag gaacacgctg aagtcgttgc gcgccgaatc
     2461 gaaagcgatg tcgctgcgtt cctcccacac gttgccgact tcccacgcca tgccgaggta
     2521 ggtcggcaga tcgaggaagg ccgtgccggg acggccgatc tggcggtaat agatcgcgcg
     2581 cgcgatggca aagtggcggc cggcgatctc gccgggcgcg agccccgaca gattgaggaa
     2641 gccgccgaga gggaagagat tgcgcacgtc accgggcgcc gagtcgagat tggagccggc
     2701 gctcgcccag aatacggcgc tgttgcgccc ccaggaacgc gcgatctgcc agtcgatgct
     2761 gacgagatcc gcatccgagg aggtcccgga caggtcgcgc tcgcctgtcc agtcgatgaa
     2821 cagcgaagtg ccggtgcgcg gaaagcccac atcgtcgagg ctgtcgtagg cgatgcgcgc
     2881 atacagctgc cggacatcga attcgtacac cggcagggcg gggtctccga cccgcacgcg
     2941 cgattcaccg tcctcgcgat gcacgccgac acgcacttcg aacgcgccgc cgaactcacg
     3001 gccgaagtcg agcccgtagc gcgcgccctt gatgcgatac tcggcgagtc gcctgccgtc
     3061 ggtatcgaaa aggccgacat tgcgcgcgcc gtactcgcct tgcggcagca cgaaccagcg
     3121 attgcggtag gacagcggca ggtagacctc ggtcgcaaca cgcggcgact cgccggcctg
     3181 caggtcccac acccactcgc cgcccagctg gttgatctct gcgagcacga agcgcgcgct
     3241 cgcgttgtag gccgagttgc cttcgaaatc gtcgcccagg ctcaggccga agcgcacgta
     3301 gttcggtccc caggagttgc gcgtcgccgt cacctcgagc ccttcccggc cggcctcgtc
     3361 gctgacgatc tggtagtcga gcgtctcgag gttaccgagg ccgtagagct cggtgacccg
     3421 cgcatcgatc gcctcgccat cgagcggtgc ccccacgaac tcctcgaaaa ggtcttcgat
     3481 cggccgctgg tagcgctgcg agccctccgc gacgcgcagg aacccgatct gcggaatttc
     3541 cctcgcgcgc acctgcgcgc gcgcgtcaac ataggcctgg tagcgcggct gcgatacggc
     3601 aagccgcgcc agcgccgatg ccgcggcacg cgtcgcgtcg gccccggcct ggatcgaatc
     3661 cgcgaagcgc tcgaaatcga acgacgacgc gttggcgagc gccgggtcga tgagtatgtc
     3721 gccgtccgtg accgacgcgc gctgcagttc gctgttgcgg cggatcagga tcgcgagcat
     3781 ctggttcgag atgatcggca cggtggagag cttgtcgcgc gtcagcagcg ggaagccggt
     3841 gtcgaccacg atcagcacgt cgacgcccat ggcgcgcgcg acgtcgacgg gcaggttgtc
     3901 cgacagcccg ccatcgacga gcaggcgccc gtcgcgctcg accggcacga acacgccggg
     3961 caccgacatg ctcgcgcgca tcgccgtggc gaggtcgccc gagtcgagga cgatgcgctc
     4021 gccggtctcg agatcggtcg ccagcgcgcg aaacggcgtc ggcagcgcgt cgaaatcccc
     4081 gatcgtcgcg gccggcagcg tgagcttgcg gaacaggtcg tgcagacgct ggccctggac
     4141 cagccccttc ggcaggcgga actcgccgcc gcgcaggccg agcggcaggc gcaccaggaa
     4201 gttgcggtcc tcctcccggc gcctgaacga gaggtcacgg cgcggcggac ggtcgccgag
     4261 cacgtcgccc cagtcgaggc cgtgcagcgc ggcgtcgatc tcggtggcgg aaaggccgct
     4321 cgcgtaaagc ccgccgacga ctgcgcccat gctcgtgccc gcgatcgcat cgatcggcac
     4381 ctgcatttcc tcgagcacct tgagcacgcc gacatgcgcc gcgccacgcg caccgccgcc
     4441 cgacagcacg agcccgacgc gcggccgcgt atcggccgcc gccgcaaaga tcggcatcac
     4501 gacacacagc caggcgatga gccgcgggac tctcacggcc gcaaggatac cagccccgca
     4561 atcgcgcata atgccgcgat gttgcgctcg agcctgcttc tgctgtcgct ggccgccgcg
     4621 gtcgcccatg gtcagtcgcc catcgtgagt gactggggtc attacggcgg cgacgtcttc
     4681 gcgcagcgct actcgtcgct caatgaaatc gatcgctcga acgtcgacaa gctcgaggtc
     4741 gcctggacct atcgcaccgg cgagctcggt gcgggcttcg aacgggccgg caagctctca
     4801 ttcgaggcaa cgccggtgct ggccttcggc ctgctgtacc tgtcgacgcc gacgaacatc
     4861 gtgatcgcgc tcgatcccgc gaccggtacc gaacgctggc gccacgatcc gcagataccg
     4921 cgcaaccagc attattcgga gggcacctct cgcggcgtca ccgtgtggga ggatccgggc
     4981 gaggctcgcc agggtgcctg ccgccgccgg gtgttcaccg gcacgctcga tgcgcgcctg
     5041 atcgccctcg atgccgagag cggcgagccc tgcaaggatt tcggccctgg tggcgccgtg
     5101 gcgctcgccc ccgatgcgcg accgaccagt ccgggcgaat ttctcgtcac ctcgccgccc
     5161 gcgaccttcg acgatgtcgt catcgtcggc tccgcgatcg gcgacaaccg cggcgtcgag
     5221 ctcgagcgcg gcatcgtgcg cggcttcgat gccaggagcg gcgcgctgct gtggcgcttc
     5281 gatccggtgc ccgattcgga aacacatccc gcctggggcg actggcgccc cgaacaggcg
     5341 cggcagacgg gcggtggcaa tgcgtgggcg ccgatcaccg tggacccggg acgcggcctc
     5401 gccttcatcc cgaccggctc ggcgagtccc gacttctacg gcggccagcg cctcggcaac
     5461 aacgcgatgg cgaattcgct cgtcgcgctc gacgcgcgca ccggcgaggt acgctggttc
     5521 cggcagctga tccaccacga cctgtgggat ttcgacctgg cagcgcagcc cacgctgatc
     5581 gagatcgagg gcgagacgcg ctcggtcgcg gcggtcgtgc aggcgacgaa gtccggcctg
     5641 gtattcgtgc tcgatcgcga gacgggcgag cccgtctacg gcatcgagga acgcaaggtg
     5701 ccggcatcgc gggtgcccgg cgaagtggcc tcccccacgc agcccttccc ctcgacaccg
     5761 ccactcgtct cgcatgcgcc gctcacggag gacgacgcct ggggactgac cttctgggat
     5821 cgcggcaagt gccgcaagct gatcgcgaaa taccgcaacg agggcgcgta cacgccaccg
     5881 gacctcgaag gcacgatcct gtatccgagc tatgtgggcg gcgtgaactg gggcggtgtc
     5941 gcgcacgacg tgcgccgcaa tcacatcatc accgtcgtga accacatccc gatggtcgtc
     6001 actctcgcgc cgcgcgaact gctgcgccgg cagcgcgaat ccggcgacta cccgcactcg
     6061 gaattctcgg cacaacgcgg caccccgtac ggcatgcgcc gcgaaccgct gttctcgccg
     6121 ttcaagctgc cctgcgtgcg cccgccctgg ggcacgttgg tcagcatcga catcgagcgc
     6181 aacgcgatcg catggcaggt gccgctcggc agcacccgcg gcgtcgcgcc cgcatggctg
     6241 cccgcacgcg atttcggcat gcccagcatg ggtgggccga tcgtcaccgc gggcgacctg
     6301 gtgttcgtcg ccgcggccat ggacaaccag attcgtgcct tcgacatcga gaccggcaag
     6361 gaactgtgga agcgggcact gcccgccggc gggcaggcca cccccatgac ctatcgcgcc
     6421 gggccggacc ggcgccagta cgttgtgatc gccgccggcg gccacggcgg gctcggcacg
     6481 cccaagggcg actatgtcat cgcctacgcg ctgccgaaat gaggcggggc gcgttcgcct
     6541 tcctgctcgc cacggcgggc gcccacgcgg ctgacgcaac gctgcaggag ttcgtcgtca
     6601 cgacgacgcg gctcccggcg ccgcgcatcg acgtgccggc cagtgtcggc ctcgtcgatg
     6661 ccggggacat cacgcgggtc ggcgccacgc actactcgga agccgtgaac cgcatccccg
     6721 gcgtctacgt gcagcgcggc ggcggccagg aaagcctggt gtcggtccgc tcgccggtgc
     6781 tgaccggcgc gggcgcctgc ggcgctttcc tcgcggccga ggacggcctc gccatccggc
     6841 cggtggggtt ctgcaacgtc aacgatctgt tcgaagtgaa caccgagcag gcgggtggca
     6901 tcgaggtgct gcgcgggccg ggctcggcaa tccatggcgc gagtgccgtt cacggcatca
     6961 tcaacatcct cacgccgtcg gtcgcggagc tgccgcgcct cggtctcgcg ctggaaggcg
     7021 ggcaggacag ctatcgccgc gtgaaggtcg cgacccgcac accgggcggc acgcggggct
     7081 ttggcaccta tggacactac acgcacgatg gcggattccg tgacgactcc ggtgtcgatg
     7141 agggcaagct gaacctgctg tacgacgagg ccgacgtggc cggcggcacg ctgcgcgtgc
     7201 gtgccgcagg aacctggctg gaacaggaga gcgcgggcta catccgcggc tacgagtcgt
     7261 acaaggacga ggcgatcgtg cagacgaacg ccgatccgga agcctttcgc gacgcgtgga
     7321 gcgcgcgcgg ctcggccgcg tggtcgcgct cgacctgcga gggctgcgcc gccgatgtgc
     7381 ggctgatcgt gcgccgctcg cagatgcagt tcctgcagca cttcctgatc ggcaaaccgc
     7441 tcgaggagaa cgcgcagtcg agcgccatgg tgtccgcctc gacgcgccgc ccactgggcg
     7501 cggcctggag ctggggggcg ggcctcgacc tcgaagcctc cgactcggat ctgcgcgaaa
     7561 cacagaacgg cccggcgacc gacggcacgc ccgccgcgaa tgcgatccgc ccggccggca
     7621 agcactacga ctatggcgtc gacgggcgca ccgccggcgc cttcgccacc ctcgggtggc
     7681 ggcccgatcc gcgctgggtg ttgaacgcgg gcctgcgcat cgagcgcaca cgctacgact
     7741 acgacaaccg catgatcgcc ggcaacaccg acgagaacgg cgtgccgtgc ggcgccgccg
     7801 gctgcctcta cagccggcct gccgaccgcg tcgacgagtt cgacaacgtg gcccccaaga
     7861 tcgacgtgat gtacctgccc cgcgagcgcc accgcctcta cctgtcgtgg tcgcgcggct
     7921 ttcgcccgcc ggagcagacg gagctctacc gcctgcagcg ccagcaggac atcgccgacc
     7981 tcgactccga gcgcctgcag ggcggcgaga tcggctggcg tgccgcaatg ggggacttcg
     8041 attggtcgct cgcggccttc gcgctcgaca agcgcaatgt catcgtgcgt gacagcagcg
     8101 gcttcaatgt caacggcggc gagaccaccc atcgcggcat cgagtacgaa ttgcggtggc
     8161 gcctgtcgcc ctactggcgc gcaagcgccg ccggcagcgt cgcctggcat cgctacgcgt
     8221 tctcggccgc cgtcgagggc ggcgagacga tcaccgacgg caacgatgtg gataccgccc
     8281 cgcgcaatgt gcacgcgctc cgcatcggct ggtcaacggc cacgaacctg gacgccgagt
     8341 tcgaagtgct gcacgtgggg cgctactggc tcgatgcctc gaac
//
LOCUS       DAOQVY010000107         8314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_9523_length_8314_cov_5.449570, whole genome shotgun sequence.
ACCESSION   DAOQVY010000107 DAOQVY010000000
VERSION     DAOQVY010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9523_length_8314_cov_5.449570"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PKN91_09830"
     CDS             <1..371
                     /locus_tag="PKN91_09830"
                     /inference="COORDINATES: protein motif:HMM:NF020752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNS28412.1"
                     /translation="MNDDFNTPQALAVLQGLARDLNSARAAGNHDTACMRAGELVALG
                     NVLGILTVDPEQWLRLAPPAASAGNVPDDAQIAALIEARLAARKAKDWAQSDRIREQL
                     AAAGIVLEDKPGGETVWRRG"
     gene            complement(388..1740)
                     /locus_tag="PKN91_09835"
     CDS             complement(388..1740)
                     /locus_tag="PKN91_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNS28413.1"
                     /translation="MSRLVCVSNRISVPRRGAAPGGLAIGVLAAMREKGGLWFGWSGE
                     TSADTGAATEVTVRDHISFATIDLTQTLYDRYYRGFCNTTLWPLFHYLTDRFRYDQEW
                     HDGYRAVNRLFAERLLPLLKPDDRIWIHDYHLIPLARVLRELGVTQPIGFFLHIPFPH
                     LEVLRLLPAFAELIRDLTYYDLVGFQTEPDELSFRASLAGVFPERAPPVRSGVFPIGI
                     DVDAVTAEATAAMRTEPVRRMVDGLLGRPLIIGVDRLDYSKGLVERFTAYQRFLERHT
                     SLRGRLTYLQIAPLSRSDIGAYAQIRQALEQAAGRTNGRFADTDWTPIRYLNRNFPHG
                     TLMGFMRASQVGLVTPLRDGMNLVAKEYVAAQDPADPGALILSSLAGAAHELTAAVIV
                     NPYDLLGVAEAIARAFAMPLEERRERHAAMLEALRRHDLHAWYRDFLASLEASRGLLR
                     "
     gene            complement(1737..3524)
                     /locus_tag="PKN91_09840"
     CDS             complement(1737..3524)
                     /locus_tag="PKN91_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNS28414.1"
                     /translation="MSASAASLDLAVIGNCSIASLIDQRGRHVFTCLPRLDGDPVFCN
                     LLGCGDSGFYDIELAGCVRSSQQYLRNTAIVETHLETADGAAIRVVDFCPRFRQFERM
                     FRPAMLVRVVEAVAGRPRVRVRLRPRLHHGAAESQVRRGSHHIRYIGDEGVDLRLTTS
                     ASLDYILEERWFQLEHSITLLLGPDESIAESVDRLGERFLSATTDYWRDWVRTLAVPF
                     EWQEPVIRAAITLKLCSYEDTGAIVAALTTSIPEAPDSGRNWDYRYCWLRDSLFTVKA
                     LNRLGATRTMESYLRFIVNTAAGESSYALQPLYGISGETAVEEREVAGLPGYRGMGPV
                     RVGNAAWRQPQNDAYGAVVLSAMQAFFDERLREKAGVDTLRSLTAIGEHAWALHDKPD
                     AGIWEFRTRTEVHTFSAAMCWAALDRLAHLHAYFGEPDAAMWRTRADTVRASVLERGW
                     NARLDSFVDVYDGSGVDASLLLLPEIGLVEPNDPRFLGTLRLIELRLRRGAFIARYDR
                     SDDFGEPAVAFNICTFWYINALAGAGRQQEARELFEEMLACRNHVGLLSEDIDPVSGE
                     LWGNFPQTYSMVGIINAAMRLSRPWESAL"
     gene            complement(3521..4285)
                     /gene="otsB"
                     /locus_tag="PKN91_09845"
     CDS             complement(3521..4285)
                     /gene="otsB"
                     /locus_tag="PKN91_09845"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HNS28415.1"
                     /translation="MIDLPRQLGWSALHGRALYLDVDGTLLDIAPSPDAVVVPGALPG
                     ALARIAQRLDGALALVSGRQIDTLDRLFHPQRFPAVGVHGAEVRADSAPATTAGHFAR
                     HLDGARAALAARMTQWPGALLEDKGLALAVHYRDAAALEGVIRETMQRLAALAGAEFT
                     LLQGKFVLELKPAALSKASGISTLRDVAPFADRAPVYLGDDVTDESAFALVNEAQGLS
                     VRVGPPAGTLARYRVDAPAAVRAWLHGLADSQEGGP"
     gene            complement(4296..5153)
                     /gene="folD"
                     /locus_tag="PKN91_09850"
     CDS             complement(4296..5153)
                     /gene="folD"
                     /locus_tag="PKN91_09850"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF010783.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNS28416.1"
                     /translation="MTARLIDGKRIATDVKSEVRAEIDSAVARGMRRPGLAVVMVGDN
                     AASAIYVRNKRKACEESGVLSVAHDLPQSTSERELLTLIDELNGDPRIDGILVQLPLP
                     DQIRQRAIIDRIDPQKDVDGFHPYNLGRLAQRSPLIRPCTPYGVIRMLDAEGLSVRGL
                     NAVVVGASNIVGRPMSLELLLMGATTTVCHRFTRDLEAHVRAAELLVVAVGKPGLVRG
                     EWIRPGAIVVDVGMNRLANGKLVGDVEFAGARERAAWITPVPGGVGPMTVAMLMRNTL
                     EASLRRQGA"
     gene            5232..5308
                     /locus_tag="PKN91_09855"
     tRNA            5232..5308
                     /locus_tag="PKN91_09855"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5266..5268,aa:Pro,seq:tgg)
     gene            5332..5408
                     /locus_tag="PKN91_09860"
     tRNA            5332..5408
                     /locus_tag="PKN91_09860"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5366..5368,aa:Arg,seq:tct)
     gene            5595..5670
                     /locus_tag="PKN91_09865"
     tRNA            5595..5670
                     /locus_tag="PKN91_09865"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5628..5630,aa:His,seq:gtg)
     gene            5681..5756
                     /locus_tag="PKN91_09870"
     tRNA            5681..5756
                     /locus_tag="PKN91_09870"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5714..5716,aa:Lys,seq:ctt)
     gene            5811..5895
                     /locus_tag="PKN91_09875"
     tRNA            5811..5895
                     /locus_tag="PKN91_09875"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5845..5847,aa:Leu,seq:tag)
     gene            5916..7223
                     /gene="tig"
                     /locus_tag="PKN91_09880"
     CDS             5916..7223
                     /gene="tig"
                     /locus_tag="PKN91_09880"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNS28417.1"
                     /translation="MQVSLTATGGLERKLEVAVPADQVSAEVDQRLKKIARTARFKGF
                     RPGKAPMGVVRQQYGSQVHSEVVNDLLRSSFADAISQQNLKPATGPRIEPIAMDPGAD
                     LKYVATFEVLPEIKLAPVESLAIERPTATVEDSDVEEMLESLRRQRPQFAVVERGAKT
                     GDRVVLDFDGRIDNVAFQGGEGRDMPVVLGAGQVLTEFEQALAGAKAGESRTAVVHFP
                     DDYHGREVAGKTAEFTITVKQVEEASLPALDDAFAAEFGVKEGGVEQLRKEVRESMER
                     ELEEAVRNHLRAQVMDALFRENPLEVPQGLVDETVQEMQVEIGRRSGAKDVSQLPPRE
                     QFIAPARRRVALGLVVGELLRGAALKVDRARVQARLEDIAGSYPNADEMRRAYLQNAD
                     AMRQIESSVLEEQLVDWVVERAKVTDKASSFKELTRFGQNSGS"
     gene            7220..7849
                     /gene="clpP"
                     /locus_tag="PKN91_09885"
     CDS             7220..7849
                     /gene="clpP"
                     /locus_tag="PKN91_09885"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNS28418.1"
                     /translation="MNEQALNLVPIVVEQTARGERAYDIYSRLLKERVVFVVGPVEDY
                     MANLIVAQLLFLESENPDKDIALYINSPGGSVSAGLAIYDTMQFIKPDVSTICVGMAA
                     SMGAVLLAGGAKGKRYMLPNSRAMIHQPWGGFQGQATDISIQAREMLRTRERLNEILA
                     RHTGQAIEKIQQDTERDNFMGGEEAIAYGLVDQLLDKRLPPATVAAQSK"
     gene            7941..>8314
                     /locus_tag="PKN91_09890"
     CDS             7941..>8314
                     /locus_tag="PKN91_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005315369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HNS28419.1"
                     /translation="MTDESRSRNDDGKLLYCSFCGKSQHEVRKLIAGPSVFVCDECVE
                     LCNDIIREELEDRADRARDKLPKPHEIKKVLDEYVIGQERAKKVLSVAVYNHYKRLQT
                     RGSSARAKEEVEIAKSNILLIGP"
BASE COUNT         1410 a         2730 c         2889 g         1285 t
ORIGIN      
        1 ccatgaacga cgacttcaac acgccgcagg cgctcgcggt gctgcagggc ctcgcgcgcg
       61 acctgaattc ggcgcgcgcg gcgggcaacc acgatacagc ctgcatgcgc gcgggcgagc
      121 tggtcgcgct cggcaacgtg ctcggcatcc tcaccgtcga tccggagcag tggttgcgtc
      181 tcgcaccacc ggcagcgtcg gcgggcaacg tgcccgacga tgcgcagatc gcggcgctga
      241 tcgaagcgcg cctcgcggcg cgcaaggcga aggactgggc gcagtccgac cgcatccgcg
      301 agcagctcgc cgccgcgggc atcgtgctcg aggacaagcc gggtggcgaa accgtctggc
      361 gccgcggcta gcccgggcgg gcacccgcta gcgcagcagg ccgcgcgagg cttcgagcga
      421 ggcgaggaaa tccctgtacc aggcgtgcag gtcatgccgg cgcagggcct cgagcatcgc
      481 cgcatggcgt tccctgcgct cctcgagcgg catcgcgaat gcgcgcgcaa tggcctcggc
      541 gacgccgagc aggtcgtaag gattgacgat cacggcggcg gtcaactcgt gcgcggcgcc
      601 ggcgagcgag gagaggatca gggcgcccgg atcggcgggg tcctgtgcgg cgacatattc
      661 cttggcgacg aggttcatgc cgtcgcgcag cggcgtgacc aggcccacct gcgaggcgcg
      721 catgaagccc atcagcgtgc cgtggggaaa gttccggttc aggtagcgga tcggcgtcca
      781 gtcggtgtcg gcaaagcggc cattggtccg tccggcggcc tgttcgagcg cctggcggat
      841 ctgggcgtac gcgccgatgt cggaacggct gagcggcgcg atctgcaggt aggtcaggcg
      901 accgcgcagg ctggtgtggc gttcgaggaa gcgctggtag gcggtgaagc gctcgaccag
      961 gcccttgctg taatcgagac ggtcgacgcc gatgatcagc ggccgcccca gcaggccgtc
     1021 gaccatcctt ctgaccggtt cggtgcgcat cgcggcggtg gcctcggcgg tcacggcgtc
     1081 gacgtcgatg ccgatcggaa acacgccgct gcgtaccgga ggcgcacgct cgggaaagac
     1141 acccgcgagg ctcgcgcgga acgacagctc gtcgggctcg gtctggaagc cgaccaggtc
     1201 gtagtacgtc aggtcgcgga tgagttccgc aaaggcaggc agcagccgca gcacttcgag
     1261 gtgcgggaac gggatgtgca ggaagaaacc gatcggctgc gtgacgccga gttcacgcaa
     1321 cacgcgcgcg agcgggatca gatgatagtc atggatccag atgcgatcgt ccggcttgag
     1381 cagcggcagg aggcgctcgg cgaagaggcg attcaccgcg cgataaccgt cgtgccactc
     1441 ctggtcgtag cggaagcgat cggtcaggta atggaagagg ggccacagcg tggtgttgca
     1501 gaagccgcgg tagtagcgat cgtacagcgt ctgtgtcagg tcgatggtcg cgaagctgat
     1561 gtggtcgcga accgtcactt cggtcgccgc gccggtgtcg gcgctagtct cgccgctcca
     1621 gccgaaccac agcccgccct tttcgcgcat cgccgcgagg accccgatcg cgaggccgcc
     1681 gggtgccgcg ccgcgccgcg ggaccgagat gcggttcgat acgcagacga ggcggctcac
     1741 aacgccgatt cccacggtct cgacaggcgc atcgccgcgt tgatgatgcc gaccatgctg
     1801 taggtctgcg ggaaattgcc ccacagctca ccgctcaccg ggtcgatgtc ctccgacagc
     1861 aggccgacgt ggttgcggca cgccagcatc tcttcgaaca gctcgcgcgc ctcctgctgg
     1921 cgtccggcgc cggccagcgc gttgatgtac cagaaagtgc agatgttgaa cgcgaccgcg
     1981 ggttcgccga agtcgtccga ccggtcgtag cgcgcgatga aggcgccacg gcgcaggcga
     2041 agctcgatca ggcgcagcgt accgaggaag cgcggatcgt tcggctcgac gaggccgatc
     2101 tccggcagca gcagcaggct cgcgtcgaca ccgctcccgt cgtagacgtc gacgaagctg
     2161 tcgagcctgg cgttccagcc gcgctcgagc acgctcgcgc gcacagtgtc ggcgcgcgta
     2221 cgccacatcg cggcgtcggg ttcgccgaag tacgcatgca ggtgagcgag ccggtcgagc
     2281 gcggcccagc acatggccgc ggaaaaggtg tgcacctcgg tgcgcgtgcg gaactcccag
     2341 atccccgcgt cgggcttgtc gtgcaaggcc caggcatgct cgccgatcgc cgtcagcgag
     2401 cgcagcgtgt cgactcccgc cttctcgcgc aggcgctcgt cgaagaacgc ctgcatcgcc
     2461 gacagcacga cggcgccata ggcgtcgttc tgcggctgac gccaggcggc gttgccgacg
     2521 cgcaccgggc ccatgccgcg atagcccggc aggcccgcga cctcgcgctc ctcgactgcg
     2581 gtctcgccgc tgatgccgta cagcggctgc agcgcgtagc tgctctcacc ggccgcggta
     2641 ttgacgatga agcgcaggta ggactccatc gtgcgggtcg cgccgaggcg gttcagcgcc
     2701 ttgacggtga acaggctgtc gcgcagccag cagtagcgat agtcccagtt gcggccgctg
     2761 tcgggcgcct cgggaatcga agtggtcaac gcggcaacga tcgccccggt gtcctcgtag
     2821 ctgcacagct tcagcgtgat cgcggcgcgg atcaccggtt cctgccattc gaacggcacg
     2881 gccagcgtgc gcacccagtc acgccagtag tcggtggtgg ccgagagaaa ccgctcgccc
     2941 agccggtcga cggactcggc gatcgattca tccggaccca gcagcagcgt gatcgagtgc
     3001 tcgagctgga accagcgctc ctcgaggatg tagtcgaggc tcgcgctggt cgtgaggcgc
     3061 aggtcgacgc cttcgtcgcc gatgtaacgg atgtggtggc tgccgcgtcg tacctgggat
     3121 tccgctgcgc cgtggtgcag gcgcggtcgc aggcgaacgc gaacgcgcgg ccggccggcc
     3181 accgcctcga cgacgcgaac cagcatcgcc ggccggaaca tgcgctcgaa ctggcggaag
     3241 cgcgggcaga aatcgacgac gcggatcgcc gccccatccg ccgtctcgag gtgcgtctcg
     3301 acgatcgccg tattgcgcag gtactgctgc gagctgcgca cgcagccggc gagttcgatg
     3361 tcgtagaagc cgctgtcgcc gcagccgagc aggttgcaga acaccggatc gccgtcgagc
     3421 cgcggcaggc aggtgaatac gtgccgcccg cgctggtcga tcagcgaggc gatcgagcag
     3481 ttgccaatga cggcgagatc gagactggcg gcgcttgcgc tcatgggcct ccttcctgcg
     3541 agtcggccag gccatggagc cacgcgcgca ccgcagccgg tgcgtcgacg cggtaccggg
     3601 cgagtgtgcc agcgggtggg ccgacgcgca ccgacaggcc ctgcgcctcg ttcacgagcg
     3661 caaacgcgct ctcgtcggtc acgtcatcgc ccaggtagac cggcgcacga tccgcgaacg
     3721 gggcgacgtc acgcaacgtc gagatgcctg aggccttcga cagcgcggcg ggtttcagtt
     3781 cgagcacgaa cttgccctgc agcagcgtga attccgcgcc cgcgagggcc gcgagccgct
     3841 gcatcgtctc gcggatcacg ccttcgagcg ctgccgcgtc gcggtagtgc acggcgaggg
     3901 cgagcccctt gtcttcgagc agcgcgccgg gccactgcgt catgcgagcg gccaatgccg
     3961 cgcgcgcccc atcgaggtgc cgtgcgaagt gcccggcggt ggtggccggc gccgaatcgg
     4021 cgcgcacctc ggcaccatgc acgccgacgg cggggaatct ctgcgggtgg aacagtcggt
     4081 cgagtgtgtc gatctgccgg ccgctcacca gtgcgagcgc gccgtccagg cgctgcgcaa
     4141 tcctcgcgag cgcgccgggc agggcgcccg gcaccacgac cgcatccggg ctcggcgcga
     4201 tgtcgagcaa ggtgccgtcg acatcgaggt acagcgcccg tccgtgcagt gcgctccagc
     4261 cgagttgccg cggcagatcg atcatcgcga ccacgtcagg cgccctggcg gcgcagcgac
     4321 gcttccagcg tattacgcat caacatggcc accgtcatcg ggccgacgcc accggggacc
     4381 ggcgtgatcc aggccgcgcg ctcgcgggcg ccggcgaact cgacgtcgcc gacgagcttg
     4441 ccgttcgcga gccggttcat gccgacatcg acgacgatcg cgcccgggcg aatccactcg
     4501 ccgcgtacga gtcccggctt gccgactgcg acgacgagca actcggccgc gcgcacgtgc
     4561 gcctcgaggt cgcgtgtgaa gcggtggcaa acagtcgtcg tcgcgcccat cagcagcagc
     4621 tcgagcgaca tcggccgccc gacgatattc gatgcaccga ccacgacggc attcagcccg
     4681 cgcaccgaca gtccctcggc gtcgagcatg cggatcacgc catagggcgt gcaggggcgg
     4741 atcaggggac tgcgctgcgc gagccggccg aggttgtatg gatgaaagcc atcgacgtcc
     4801 ttctgcgggt cgatgcggtc gatgatcgcg cgctggcgga tctggtcggg cagcggcagc
     4861 tgcacgagga tgccgtcgat gcgcggatcg ccgttcagtt cgtcgatcag ggtcagcagc
     4921 tcgcgctcgg aggtcgactg cggcaggtca tgcgcgaccg acaggacgcc cgactcctcg
     4981 caggccttgc gcttgttgcg cacgtagatg gccgaggccg cgttgtcgcc caccatgacc
     5041 accgccaggc ccggacggcg catgccgcgc gcgaccgccg agtcgatctc ggcccgcacc
     5101 tccgatttga cgtcggtggc gatgcgcttg ccatcgatga gcctcgcggt catcgatact
     5161 cctttgccgg aatgggcgcg cattttacgg gattgtgcgt aaaattgagg attgttcacg
     5221 caagccggca tcggggcatg gcgcagtctg gtagcgcatc tgctttggga gcagagggtc
     5281 gcaggttcga atcctgctgc cccgaccatt tcgcgtgagg gggcctgcca cgcgcccgta
     5341 gctcagctgg atagagcacc ggccttctaa gccgggggtc gcaggttcga gtcctgccgg
     5401 gcgcaccaac caccgggtca ccggatttcg gagccaggac gacggatgaa gtaaggtgcc
     5461 ggcccgctgc gctcccgcct cctgatcggt acggcagttc gtcgccgtcc cggtgtcgtt
     5521 gtttggtgcg atatttctcg cgctgtagcg ctggcggcgg gcgctggtat actcccgctc
     5581 ccctcagggt gacggtggac gtagctcagc tggtagagcc ccgggttgtg atcccggccg
     5641 tcgtgggttc gagtcccatc gtccacccca gatccggtaa gggccgttag ctcagctggt
     5701 agagcaggag actcttaatc tcttggtcgt aggttcgact cctacacggc ccaccaccct
     5761 tttggctata atccgcgccc cctcgcacag ggtcgagctc cgcagccaga gcgaaagtgg
     5821 cggaactggt agacgcgctg gatttaggtt ccagtggggc aacccgtgag agttcgagtc
     5881 tctcctttcg caccagaaat tcaggatagg gcacgatgca ggtttcattg acggcaaccg
     5941 gtgggctgga gcgcaagctt gaggtcgccg tcccggccga ccaggtcagc gccgaagtcg
     6001 accagcggct gaagaagatc gcgcgtaccg cgcgcttcaa gggtttccgc ccgggcaagg
     6061 cgccgatggg tgtcgtgcgc cagcagtacg gctcgcaggt ccacagcgaa gtggtcaacg
     6121 acctgctgcg ctcctcgttc gccgacgcga tctcgcagca gaacctgaag ccggcgaccg
     6181 ggccgcgcat cgagccgatc gcgatggatc cgggcgccga cctcaagtac gtcgccacat
     6241 tcgaagtgct gccggagatc aagctggcgc cggtcgagtc gctcgccatc gagcgcccga
     6301 ctgcgacggt cgaggattcg gacgtcgagg aaatgctcga gtcgctccgc cggcagcgcc
     6361 cgcagttcgc cgtggtcgag cgcggcgcga agaccggcga tcgcgtggtg ctcgatttcg
     6421 acggccgcat cgacaacgtg gctttccagg gcggcgaggg ccgtgacatg ccggtcgtgc
     6481 tcggcgcggg gcaggtgctc accgaattcg agcaggcgct tgcgggcgcg aaggccggcg
     6541 agtcccgcac tgccgtcgtg cacttcccgg acgactacca cggcagggaa gtggcgggca
     6601 aaaccgcgga attcacgatc acggtgaagc aggtcgagga ggcgtccctg ccggcgctcg
     6661 acgatgcgtt cgccgcggaa ttcggcgtca aggaaggcgg cgtcgagcag ttgcgcaagg
     6721 aagtgcgcga aagcatggag cgcgagctcg aagaggcggt gcgcaaccat ctgcgcgcgc
     6781 aggtcatgga cgcgctcttt cgcgagaacc cgctcgaagt gccgcagggg ctcgtcgacg
     6841 aaaccgtgca ggaaatgcag gtcgaaatcg gccggcgctc cggcgcgaag gacgtgtccc
     6901 agctgccgcc gcgcgagcag ttcatcgctc ccgcccgccg tcgcgtggcg ctcggcctgg
     6961 tggtcggcga gctgctgcgc ggtgccgcgc tcaaggtcga tcgtgcacgc gtgcaggcgc
     7021 gcctcgagga catcgcgggc agttacccca atgccgacga gatgcgccgc gcctacctgc
     7081 agaacgccga cgccatgcgc cagatcgagt cctcggtcct cgaggagcag ctggtcgatt
     7141 gggtcgtgga acgggcgaag gtgacggaca aggcatcttc cttcaaggaa ctgacccgct
     7201 tcgggcagaa tagcgggtca tgaacgagca agccctgaac ctggtgccga tcgtcgtcga
     7261 gcagaccgcc cgcggcgagc gtgcctatga catctattcg cgcctcctga aggagcgcgt
     7321 ggtgttcgtc gtgggtcccg tggaggacta catggcgaac ctgatcgtcg cgcagctgct
     7381 gttcctcgag tccgagaatc cggacaagga catcgcgctt tacattaatt cgcccggcgg
     7441 ctcggtcagt gccggactcg cgatctacga cacgatgcag ttcatcaagc ccgatgtgag
     7501 caccatctgc gtcggcatgg cggcctccat gggcgccgtg ctgctggctg gcggcgccaa
     7561 gggcaagcgc tacatgctgc cgaactcgcg cgcgatgatc caccagccct ggggcggctt
     7621 ccagggccag gcgaccgaca tcagcatcca ggcccgcgaa atgctgcgca cgcgcgagcg
     7681 attgaacgaa atcctcgccc gtcacaccgg ccaggcgatc gagaagatcc aacaggacac
     7741 cgagcgcgac aatttcatgg gcggcgagga ggccatcgcc tacggcctcg tcgaccagtt
     7801 gcttgacaag cgcctgccgc cggccacggt tgccgctcag tccaagtgaa tcaatggttt
     7861 gggcgtgact ggggcgtttt gcgcccccgg tcaccccatg ctatataaga gccttgcacc
     7921 atggttacag ggcctactga atgaccgacg agtcccgcag tcgcaacgac gacggcaagc
     7981 tgctgtattg ctccttctgc ggcaagagcc agcatgaggt gaggaagctg atcgccggcc
     8041 cgtcggtgtt cgtttgcgac gaatgcgtcg agctctgcaa cgacatcatc cgcgaggaac
     8101 tcgaggatcg ggccgaccgc gcgcgcgaca agctgccgaa gccccacgaa atcaagaagg
     8161 tcctcgacga atacgtgatc ggccaggagc gcgccaagaa ggtgctctcg gtggccgtct
     8221 acaaccacta caagcgcctg cagacccgcg gcagcagtgc ccgcgccaag gaagaggtcg
     8281 agatcgcgaa gagcaatatc ctgctgatcg gccc
//
LOCUS       DAOQVY010000108         8303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_9538_length_8303_cov_5.899006, whole genome shotgun sequence.
ACCESSION   DAOQVY010000108 DAOQVY010000000
VERSION     DAOQVY010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9538_length_8303_cov_5.899006"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="PKN91_09895"
     CDS             complement(<1..877)
                     /locus_tag="PKN91_09895"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNS28420.1"
                     /translation="MSLDLARPVAIIAWLLMMAAPAAAERRAELVLRGGAIYTMDAAR
                     SWASAVAIADGRILYVGDDAAVAAHTGRDTHVIDLGGRMVLPGFHDSHMHPLSGGLRL
                     VRCQLAGARSPEAIHAAVRACAAASEDREWLVGGGWEPSSFAAGRPHRAILDRLVPGR
                     PVYLATEDGDTAWVSSRALAAAGIDASTPDPPGGRIERDPTTGAPTGVLRGAAVSLVR
                     RCIPPPTDAEYREGLRRSLAMANRFGITSVIDASADAAMLEQYRAADRAGALSVRVLA
                     SQRVDLGRGAEQVGEL"
     gene            complement(970..2946)
                     /locus_tag="PKN91_09900"
     CDS             complement(970..2946)
                     /locus_tag="PKN91_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNS28421.1"
                     /translation="MTGISRISMAALLAFALAACGGDSGNGDGGGGGGGGGGDDGGSG
                     GSGSGTPSATYAVGGTVTGLTGTGLVIRNNGTESLTIDASGAFAFSTELADGEVYAVE
                     VTTQPANPDNFCTVDNGEGIIDGADVGDVEILCTGPLSLVSTAPADGDPAVSRAVEPI
                     LTFSADIDAATAAAAGSIRLESDAGEVDLGIDVTGAAVTLSPASPLLPLTDYRLTVTT
                     AVEGAGGERMAEPVTAEFRTRDGAWSADLDIGPGTADTAEAQIAFDHEGNALAVWSQA
                     TLDSTDLWWNYYAVGAGWGTPAPLELNDDDLYAGGVQFGFDGQGHALAVWIVSDSVAN
                     ATSVRASRFAPGTGWETPQYIENDPGTVIGYLDMAINANGDAVAAWSQFDGTTMNLWS
                     ARIEGGGSWSAPEIADESDGVTGSPSVAIDEDGDFAVLWPMRDQGSFNWKIWARNFRT
                     SGGWGSSTMLSGINNLASESPRIAMDREGNALAVWFQGLGLESGVFASRYEEGAGWLQ
                     QEQISVGAQLEDLEIAFGRDGNAIAVWGQWVDENGQQGLAVHASRYVAGTGWEAVQSI
                     SSAQQLYPKIAVDASGNALAAWFEVDLTLPNSEAFSVWGNRYVNGGGWTAPERIGSYG
                     SLIGTAMPNLAIDPNGDAFVIWTRGSEVRVNRFE"
     gene            complement(3024..5726)
                     /locus_tag="PKN91_09905"
     CDS             complement(3024..5726)
                     /locus_tag="PKN91_09905"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNS28422.1"
                     /translation="MDEPLSRQAEPIFLAASELPPAERAAHLDRACAGNAGLRREVEA
                     LLDAAERSSDYFEKLPGRLGVASFMSGDAATDPEAPLAGEAGQQFGQYRLTAPVGRGG
                     MGAVWRAVRADGRYETDVAVKLLSRTAGGAPERFALEARYLAKLAHPNIARLLDAGVG
                     PNGQPYLVLELVDGQPIDRYCEQHALGVRERITLFLAVLDAVAHAHAHLIVHRDIKPS
                     NVQVAADGTVKLLDFGVAKLLGGEAAAPGLTRELGTALTPEFAAPEQLSGDAITTATD
                     IYALGLLLWLILTGRNPREAAGACTLAELRAQAAREPAPLADAVPDASPELRRALQSD
                     LDNIVRKALAVEPAERYATAADFRQDLQRYLRDEPVMAQAQTIGYRARKFVRRHRGGV
                     LAAALTTIALVGTTAIATWMGIEARRQRDVAIYQQQRVQATNEFLNLLMNEIGPGGKP
                     LTMTELLERGVEMLDRQYGAGGEFLGRMYFELANAHFSLGDTARLAELLGRAEEQARA
                     QDDADLLAAVLCTAARARMRAEPEAAAARIAAARQALARADVVSVDSRVSCDRAEAQA
                     LELEGDRPAAIAELRATLDALAANPAASVQHRLYVMNELSRLTFNERRLVETLALNEE
                     TLDLMTRSGRGGSLGYVVNAFNGSMVLHSLGEIRRAHDLQLGLLERVEALERDGVQIG
                     FGGYYAASLLRLARCEEALPIIEQERDMLRESGNLFGAAAQELTLGRLMVCLGRFAAA
                     AAHLDAVEPVLEKAPGSNERLLALLAVTRAQVALGRGEPDRAKALVDAELARVGYPED
                     RSGPDLLHLLRIAGEIALVRGDAAAAETYAADGHAAALRVARDPAQSADVGQALLLRA
                     TARKAQGNAAGARSDLEEAVTSLANGLGEEHPDTKRARALLPAA"
     gene            complement(5748..6326)
                     /locus_tag="PKN91_09910"
     CDS             complement(5748..6326)
                     /locus_tag="PKN91_09910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HNS28423.1"
                     /translation="MSTGPVTQLLDAARRGEVGASQALFSAVYAELRTLARSQRRRWR
                     GNETMNTTALIHEAYLKLHAQGVAGFENHKHFFATASKAMRQVLVNYAEQQAAGKRGG
                     NALRVTLDEVTLASEVSADELLDLDRLLKDLEAEDPRRCQVVECRVFGGMTVAEVAEV
                     LGISEATVKREWQVACAQIFRRLEVMPAPDSG"
     gene            complement(6533..6874)
                     /locus_tag="PKN91_09915"
     CDS             complement(6533..6874)
                     /locus_tag="PKN91_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HNS28424.1"
                     /translation="MTPFSSSMALAWGLLAVAGLLEVLWLVALNVAEQLSRPLSAALS
                     LLIAGIEFLILSYVVRVIPAGTAYAVCMGLGAAGGVLVGILFFSEPMKAVQAVCVGLI
                     VVGLVGIKATQ"
     gene            complement(7164..>8303)
                     /gene="guaA"
                     /locus_tag="PKN91_09920"
     CDS             complement(7164..>8303)
                     /gene="guaA"
                     /locus_tag="PKN91_09920"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNS28425.1"
                     /translation="VEALPQGFTAVGASANAPLAAMADEARRYYGVQFHPEVTHTRQG
                     ARLLERFVRDVCGCAALWTVGNIIDDAIARVRAQVGEGRVLLGLSGGVDSSVVAALLH
                     RAIGDQLVCVFVDHGLLRLDEGDQVMRTFAENLGVRVIRVNAAQRFFTALAGATDPEQ
                     KRKIIGRLFIEVFDEEAHKLEGIGFLAQGTIYPDVIESAGARTGKAHVIKSHHNVGGL
                     PANMRMQLVEPLRELFKDEVRELGVALGLPREMVYRHPFPGPGLGVRILGEVRPEFAV
                     LLQRADAIFIDELRRHGWYDKTSQAFAVFLPVRSVGVMGDGRRYDYVIALRAVETIDF
                     MTAHWAHLPYDFLDVVSRRIVNEVRGISRVVYDISGKPPATIEWE"
BASE COUNT         1187 a         3040 c         2863 g         1213 t
ORIGIN      
        1 ctagctcgcc cacctgctcg gcgccgcgcc cgaggtcgac gcgctgcgac gccagcacgc
       61 gcacgctcag cgcgccggcg cgatccgcgg cccggtactg ctcgagcatg gcggcgtcgg
      121 cgctggcgtc gatcaccgag gtgatgccaa agcgattggc catggccaga gagcgccgca
      181 gaccttcgcg atactcggcg tcggtcggtg gcgggatgca ccgccggacc aggctgaccg
      241 cggcgccgcg caggacgccg gtcggcgcgc ccgtggtcgg gtcgcgctcg atccgtccgc
      301 ccggcgggtc gggcgtgctg gcgtcgatcc cggctgcggc cagggcccgc gagctcaccc
      361 aggccgtgtc accgtcctcg gtggcgaggt acaccgggcg acccgggacc agcctgtcca
      421 ggatcgcccg atgcgggcgc ccggcggcga agctcgatgg ctcccagcca ccgcccacga
      481 gccattcgcg gtcctcggac gcggcggcgc acgcgcgcac cgccgcgtgg atggcctcgg
      541 gcgagcgggc gcccgcgagc tggcagcgca cgagcctgag cccgccgctc agcgggtgca
      601 tgtgcgagtc gtgaaagccg ggcaggacca tgcgcccgcc gagatcgatg acgtgcgtgt
      661 cgcggcccgt gtgcgccgcg actgcggcgt cgtcgccgac gtagaggatc cggccgtccg
      721 cgattgccac tgcgctcgcc cagcttcgcg ccgcatccat ggtgtagatc gcgccgccac
      781 gcagcaccag ttcggcgcgc cgctccgcgg cggccggcgc ggccatcatc agcaaccaag
      841 cgatgatcgc gaccggtctc gccagatcga gtgacatctc cacctcgcca ttctgcagga
      901 acgcagcggg cgcaggtgtc gatgtcgatg ccgaacacgc gcttctggca gagggatcgc
      961 ccctgccgct cactcgaacc ggttcacccg cacctccgag ccgcgcgtcc agatgacgaa
     1021 ggcgtcgcca ttcgggtcga tcgcgaggtt cggcatcgcc gtgccgatga gcgacccgta
     1081 gctgccgatc cgctccggag cggtccatcc gccgccattg acgtagcggt tgccccagac
     1141 gctgaaggct tccgagttcg gcagggtcag atccacctcg aaccaggccg cgagcgcgtt
     1201 tccgctcgcg tcgactgcga tcttgggata gagctgttgg gcgctgctga tggactgcac
     1261 agcttcccag cccgtgcccg caacatagcg actcgcgtgc accgcgagtc cctgctggcc
     1321 attttcgtcg acccattggc cccagaccgc gatcgcattg ccgtcgcggc cgaacgcgat
     1381 ttcgaggtcc tccagctgcg cgccgacgct gatctgctcc tgctggagcc agccagcccc
     1441 ctcctcatag cggctggcga acacgccgct ttcgagcccg aggccctgga accagaccgc
     1501 cagggcgttg ccctcgcgat ccatggcaat ccgcgggctc tcggaggcca agttgttgat
     1561 cccggacagc atcgtggaac tgccccaacc gcctgatgtc ctgaagttcc gcgcccagat
     1621 cttccagttg aagctgccct ggtcgcgcat cggccagagc accgcgaagt ccccgtcctc
     1681 gtcgatcgca acgctcgggg agccggtcac gccgtcggac tcgtcggcga tctcgggcgc
     1741 gctccagctc ccgccgccct cgatacgtgc cgaccacagg ttcatggtcg tcccgtcgaa
     1801 ctgcgaccag gccgcgaccg cgtcgccgtt cgcgttgatc gccatgtcta agtagccgat
     1861 gaccgtgccg gggtcgttct cgatgtactg cggcgtttcc cagccggtgc cgggcgcaaa
     1921 gcggctcgcg cggacgctcg tggcattggc cacggaatcg ctgacgatcc acaccgcgag
     1981 cgcgtgcccc tggccgtcga agccgaactg cacgccgccc gcatagaggt cgtcgtcgtt
     2041 cagctcgagc ggcgcgggcg taccccagcc ggcgccgacg gcgtagtagt tccaccacag
     2101 gtccgtggaa tcgagcgtcg cctggctcca cacggcgagc gcgttgccct cgtggtcgaa
     2161 tgcgatctgc gcttccgccg tgtcggccgt gccggggccg atgtccaggt ccgccgacca
     2221 ggcgccgtcg cgcgtgcgga actccgcggt gaccggctcc gccatccgct cgccgcccgc
     2281 gccctcgacc gccgtcgtca cggtcagcct gtagtcggtg agcggcagca gcgggctcgc
     2341 cggcgacagc gtgaccgccg cgcccgtgac gtcgatgccg aggtccactt cgccggcgtc
     2401 gctctcgagg cggatgcttc cggccgccgc cgcggtcgcg gcgtcgatgt ctgccgagaa
     2461 cgtgaggatc ggctcgaccg cgcgggagac cgccggatcg ccgtcggccg gcgcggtcga
     2521 gacgagcgag agcggcccgg tgcagaggat ctcgacgtcc ccgacgtcgg cgccgtcgat
     2581 gatcccttcg ccgttgtcga cggtgcagaa gttgtcgggg ttggcgggct gcgtcgtgac
     2641 ttccacggcg tagacctcgc cgtcggcgag ctccgtggag aatgcgaagg ctcccgatgc
     2701 atctatcgtc aggctctcgg tgccgttgtt gcggatgacg aggcccgtgc cggtgaggcc
     2761 ggtcaccgtg ccgccgacgg cataggtggc gctcggcgtg ccgctgccgc tgccgccact
     2821 tccgccgtcg tctccgcctc cgcctccgcc acctccgccg ccatcgccgt tgccgctatc
     2881 gccgccgcag gctgcgagcg cgaacgccag cagcgcggcc atcgaaatcc gggagatgcc
     2941 tgtcaccgcc gttctcctgc atggaactgc ctttgcagtc catacagggt cgaaggcgta
     3001 accggctcac cggggggaag cggctatgcc gccggcagca gcgcccgcgc ccgcttcgtg
     3061 tccgggtgct cctcgccgag cccgttcgcg agcgaggtga cagcctcttc gaggtcgctc
     3121 cgcgcgcccg ccgcattgcc ctgcgccttg cgcgccgtcg cgcgcagcag caacgcctgg
     3181 cccacgtccg cgctctgcgc cgggtcgcgc gcgacgcgca gcgccgccgc gtggccgtcg
     3241 gccgcgtagg tttccgcggc cgcggcgtcg ccccgcacga gcgcgatctc tcccgcgatg
     3301 cgcagcaggt gcaggagatc gggtccggac ctgtcctccg ggtaaccgac gcgcgcaagc
     3361 tcggcgtcca ccagcgcctt cgcccggtcc ggctcgccgc ggccgagcgc gacctgcgcg
     3421 cgcgtcaccg cgagcagcgc cagcaaccgc tcgttgctgc cgggcgcctt ttcgagcacc
     3481 ggctcgacgg catcgaggtg ggcagccgcg gcggcgaagc gcccgaggca caccatcagc
     3541 cggccgagcg tcagctcctg cgcggcagcg ccgaagaggt tgccggattc gcggagcatg
     3601 tcacgctcct gctcgatgat cggcagcgcc tcctcgcagc gcgcgaggcg aaggaggctc
     3661 gcggcgtagt acccaccgaa gccgatctgc acgccgtcgc gctcgagcgc ctcgacgcgc
     3721 tcgagcaggc cgagctggag gtcgtgcgcg cgccggatct cgccgagcga gtgcagcacc
     3781 atgctgccgt tgaaggcgtt caccacgtag cccagcgagc cgccgcggcc ggatcgcgtc
     3841 atgaggtcga gcgtctcctc gttcagcgcg agcgtctcga cgagccggcg ttcgttgaag
     3901 gtcaggcggc tcagctcgtt catgacgtac agccggtgct gcacggaggc cgccgggttc
     3961 gcggcgagcg catcgagcgt cgcgcgcagc tccgcgatcg cggccgggcg gtcgccctcg
     4021 agctcgagcg cctgcgcctc ggcgcggtcg caggacacgc ggctgtcgac cgagacgacg
     4081 tccgcccggg cgagcgcctg ccgcgccgcc gcgatccgcg ccgccgcggc ctccggctca
     4141 gcccgcatcc gcgcccgcgc cgcggtgcac agcaccgccg cgaggagatc ggcatcgtcc
     4201 tgggcgcgcg cctgttcctc cgcgcggccg aggagctccg cgaggcgcgc agtgtccccg
     4261 aggctgaagt gcgcattcgc gagctcgaag tacatgcgcc cgaggaactc gcccccggcg
     4321 ccgtactggc gatccagcat ctcgacgccg cgttcgagca gctccgtcat cgtcagcggc
     4381 ttgccgccgg ggccgatctc gttcatcagg aggttcagga actcgttcgt cgcctgcacg
     4441 cgctgctgct ggtagatcgc gacatcgcgc tggcggcgcg cctcgatgcc catccaggtc
     4501 gcgatggcgg tggtgccgac cagcgcgatc gtcgtcagcg ccgcggcgag cacgccgccg
     4561 cgatggcgcc gcacgaactt gcgcgcgcgg tagccgatcg tctgcgcctg cgccatgacc
     4621 ggctcgtcgc gcaggtaacg ctgcaggtcc tgacggaagt ccgcggcggt cgcgtagcgc
     4681 tcggcgggct cgaccgcgag cgccttgcgc acgatgttgt cgaggtcgct ctgcagcgcg
     4741 cggcgcaact ccggggatgc gtccggcacg gcgtccgcga gcggcgcggg ctcacgggcg
     4801 gcctgcgcgc gcagctctgc gagtgtgcag gcgcccgccg cctcgcgtgg attgcggccg
     4861 gtcaggatca gccacagcag caggccgagc gcgtagatgt cggttgcggt cgtgatcgca
     4921 tcgccgctca gctgctcggg cgcggcgaac tcgggcgtca gcgccgtgcc gagctcgcgc
     4981 gtgagccccg gcgcggcggc ctcaccgccg agcagcttcg cgacgccgaa gtcgaggagc
     5041 ttcaccgtcc cgtccgccgc gacctgcacg ttcgagggct tgatgtcgcg gtgtacgatc
     5101 aggtgcgcgt gcgcgtgcgc gacagcgtcc agcaccgcga ggaagagcgt gatccgctcg
     5161 cgcacgccga gcgcgtgctg ctcgcagtag cggtcgatcg gctggccgtc cacgagctcg
     5221 agcacgaggt agggctggcc gttcggaccg acgccggcgt cgagcaggcg cgcgatgttc
     5281 ggatgcgcaa gctttgcgag atagcgggct tccagcgcaa agcgctccgg cgcgccaccc
     5341 gccgtgcgcg agaggagctt cacggcgacg tccgtctcgt aacgcccgtc tgcgcgcacc
     5401 gcgcgccaca ccgcgcccat gccaccgcgg ccgaccggcg cagtcaggcg gtactggccg
     5461 aactgctggc cggcctcgcc ggcgagcggc gcctccgggt ccgtagccgc atcgccgctc
     5521 atgaagctcg cgacgccgag ccggcccggg agcttctcga agtaatcgga ggagcgctcc
     5581 gcggcgtcga gcagcgcctc cacctcgcgc cggagccccg cgttgccagc gcaggcgcga
     5641 tcgaggtgag ccgcgcgttc ggcgggcggc agctcgctcg cggcaagaaa gatcggctcg
     5701 gcctgccgtg acagcggttc gtccatgctt gccgtcacat accggattca gccggagtcc
     5761 ggcgcaggca tcacctcgag gcgccggaat atctgcgcgc aggcgacctg ccactcacgc
     5821 ttgaccgtcg cctccgagat cccgagcacc tcggcgactt cggccaccgt catgccgccg
     5881 aacacgcggc actccaccac ctggcagcgg cgcgggtcct cggcctccag gtccttcagc
     5941 aggcggtcga ggtcgagcag ctcgtccgcg ctgacctcgc tcgcgagggt gacctcgtcg
     6001 agcgtgacgc gaagcgcgtt gccaccgcgc ttgccggcgg cctgctgctc ggcgtagttg
     6061 acgagcacct ggcgcatcgc cttcgaggcg gtcgcgaaga agtgcttgtg gttctcgaag
     6121 cccgcgacgc cctgcgcgtg cagcttcagg taggcctcgt gaatgagcgc ggtcgtgttc
     6181 atcgtctcgt tgccgcgcca gcggcggcgc tgcgagcgcg cgagagtccg gagctccgcg
     6241 tacaccgcgc tgaagagcgc ctgtgaggcg ccgacctcac cgcgccgcgc ggcgtcgagg
     6301 agctgcgtga ccgggccggt actcatgcgg tcaagaatac agccatcgtg agccgccccg
     6361 gcaccggatc cggtataccc cactggagga cacggccaag cacaccctgg aatgggcttt
     6421 tgcggcggcg ctgcagggcg gcggcgtcac cggcgggtac gcgagctcgc ggcgcgcgtt
     6481 ccaggcctcc aacgtctcga gccgcgggtc gtgctgacgc cgacctggcg gcctactgcg
     6541 tcgccttgat gccgacgagc ccgacgacga tcagtccgac gcagaccgcc tgcacagcct
     6601 tcatcggctc gctgaagaag aggatgccga ccagcacgcc accggcggcg ccgaggccca
     6661 tacagaccgc gtaagccgtg cccgcgggga tcacgcgcac gacgtagctc aggatcagga
     6721 actcgatgcc ggcgatcagg aggctgagcg cggcgctgag cgggcgcgag agctgctccg
     6781 cgacgttcag cgcgaccagc cagagcacct cgaggaggcc ggcgacggcc aggaggcccc
     6841 aggcgagggc catcgagctg gagaacggag tcatgcccgg aaataccggg aacggggacg
     6901 ttccggctca ggcccccggc acgacgcgtc cgggcttgcc gaggtgcgcg ccaaagttgc
     6961 cgatgagcgc gtggcagccg cacacaggca gcggctggca tggggcgcgt accggaatct
     7021 ggcagggact acgcaggcta tgccggcagc ctccagaatc ggcaaacaca atcgcctgtg
     7081 ttcgtacaga aatgccggta tctcgccgaa ttcctgaagc tgccatcagc tcagcgcatg
     7141 tgcatcgtcg caccgcgcgc ccctcactcc cactctatcg tcgccggcgg cttgcccgag
     7201 atgtcgtaga ccacccgcga tatcccccgc acctcgttca cgatccgtcg cgagacgaca
     7261 tcgaggaagt cgtacggcag atgcgcccag tgcgccgtca tgaagtcgat cgtctcgacc
     7321 gcgcgcagcg cgatcacgta atcgtagcgc cggccgtcgc ccatcacgcc gaccgagcgc
     7381 accggcagga acaccgcgaa cgcctggctc gtcttgtcgt accagccgtg gcggcgcagt
     7441 tcgtcgatga agatcgcgtc tgcgcgctgc agcaacaccg caaactccgg cctcacttcg
     7501 cccaggatgc gcacgccgag gccgggaccc gggaacggat ggcgatagac catctcgcgc
     7561 ggcaggccga gggcgacgcc cagttcgcgc acttcgtcct tgaacagttc gcgcagcggc
     7621 tcgacgagct gcatgcgcat gttcgccggc agcccgccga cgttgtggtg cgacttgatg
     7681 acgtgcgcct tgccggtgcg tgcgcccgcc gactcgatca cgtcggggta gatcgtaccc
     7741 tgcgcgagaa agccgatgcc ctcgagcttg tgcgcctctt cgtcgaacac ctcgatgaac
     7801 agccgcccga tgatcttgcg cttctgttcg gggtcggtgg caccggcgag cgcggtgaag
     7861 aaccgctgcg cggcgttcac gcggatcacc cgcacgccga ggttctcggc gaaggtgcgc
     7921 atcacttggt cgccctcgtc gaggcgcagc aggccgtggt cgacgaatac gcagacgagc
     7981 tgatcgccga tcgcccggtg cagcagcgcc gcgacgacgg atgaatcgac gccgcccgac
     8041 agacccagca gcaccctgcc ctcacccacc tgcgcacgca cgcgcgcgat cgcgtcgtcg
     8101 atgatgttgc ccacggtcca gagcgccgca cagccgcaga cgtcgcgcac gaagcgctcg
     8161 agcaggcgcg cgccctgcct cgtgtgggtc acttccggat ggaactgcac gccgtagtag
     8221 cggcgcgctt cgtcggccat cgcggcgagc ggcgcgttgg cgctcgcgcc caccgcggtg
     8281 aagccctgcg gcaacgcctc gac
//
LOCUS       DAOQVY010000109         8278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_9574_length_8278_cov_5.755077, whole genome shotgun sequence.
ACCESSION   DAOQVY010000109 DAOQVY010000000
VERSION     DAOQVY010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8278
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9574_length_8278_cov_5.755077"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PKN91_09925"
     CDS             complement(<1..938)
                     /locus_tag="PKN91_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNS28426.1"
                     /translation="MSKSSKPSLAASTTGAGAPAASDRNSLTVGADGPIVLHDVHFLE
                     QMAHFNREKVPERQPHAKGSGAFGVFRTTEDVSRYTKAAVFQRGASTEMLARFSTVAG
                     EAGSPDTWRDVRGFALRFYTDEGNYDLVGNNTPVFFVRDTMKFPHFIRSQKRLPDSGL
                     RDNHMQWDFWTSNPESAHQVTYLMGQRGLPRTWRHMNGYGSHTYMWVNAQGEKFWVKY
                     HFHTQQGMQFFSNAEAAAMAGQDADFHRRDLFEAIARSEFPSWVMSVQVMPYAAAKNY
                     RFNPFDLTKTWSHRDYPLIKVGTMTLNRNPENFFAQ"
     gene            1038..1457
                     /locus_tag="PKN91_09930"
     CDS             1038..1457
                     /locus_tag="PKN91_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28427.1"
                     /translation="MNAPTFSTFSAIAELFVTAGVFYIVLHNYARKGFAWRLATALIV
                     FEFSVNMMYMIFRMRETTAGHESAGPNPLAPFMAAHGILSLLVFLLLVVYSYLAFAAS
                     RRGRFFFADHPAVAWSFLLLWTLSVGSGWALYFINYF"
     gene            complement(1444..2007)
                     /locus_tag="PKN91_09935"
     CDS             complement(1444..2007)
                     /locus_tag="PKN91_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28428.1"
                     /translation="MKLHAAGPLFTPQKSRATQRWRSPLLLVTLGGLLFALLSGATLL
                     FMPMSPIRRDFWVFAHWTGALATIAPYALYQLRHYARVRPFVMRAPYRVGVHAFFMIC
                     GTLLTGLPLVTPLVAGTRLYTMVDLAHIFFGFVFVLLVSAHLALVGAYTLAQEPDATA
                     ARAAVRHALIGMCLIALALLYATIRSS"
     gene            2074..2544
                     /gene="tadA"
                     /locus_tag="PKN91_09940"
     CDS             2074..2544
                     /gene="tadA"
                     /locus_tag="PKN91_09940"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNS28429.1"
                     /translation="MSSTPHRTDVDFMRRALVLAGEAESHGEVPVGAVLVRGEEIVGE
                     GFNRPIGGVDPTAHAEIEALRAGAQALGSYRLTGTTLYVTLEPCLMCAAAIVHARVRR
                     VVFGAWDPKAGAAGSIIDAFALPAMNHRVDTFGGVLAEECGELLRRFFAARRGP"
     gene            2541..3086
                     /locus_tag="PKN91_09945"
     CDS             2541..3086
                     /locus_tag="PKN91_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNS28430.1"
                     /translation="MSALRSVCVYCGSQKGASPAFAQAAADLGTALAREGLTLVYGGA
                     SVGLMGVVADAALAAGGRVIGVIPRILMRKELAHAGVTELVVTESMHERKAQMAARSD
                     AFIALPGSIGTLDELFEMWTWAQLGLHAKPCVLVNTDGYYDGLIEFLGRAEQEGFLRP
                     TTRALLRIVRELPSPRAVLNP"
     gene            3222..3743
                     /locus_tag="PKN91_09950"
     CDS             3222..3743
                     /locus_tag="PKN91_09950"
                     /inference="COORDINATES: protein motif:HMM:NF038126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxDxF family PEP-CTERM protein"
                     /protein_id="HNS28431.1"
                     /translation="MIKKLRLAFAGLALIGSGIAHSAIIDLGSVTAPGALTVANSWGV
                     APGTFTDQHLFTLTNAADSYGGMIELDWFFGNTNVYSVALVGLNGTSYFGLDLDPVST
                     LWEPISLAFSGLSAGAYSLVVSGAAYGFATGYEGVLAFWPASSASVPEPSTLALLGFG
                     LIALGVMRRRLSA"
     gene            complement(3810..5249)
                     /gene="mltF"
                     /locus_tag="PKN91_09955"
     CDS             complement(3810..5249)
                     /gene="mltF"
                     /locus_tag="PKN91_09955"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HNS28432.1"
                     /translation="MRRFRGPSWWRTLGVLTALAGLAQCSAPPSNLEQIRARSELRVG
                     TLNLPTTYYFGAQGPEGIEYQLARGFADSLGVRLAMTPFASESELLEALRSGAVDIAA
                     AQLTYRDRWRLFALPSRNYDQVVQLVVYARGTVKPGKTRELVGRRIVVKAESAQVGVL
                     EALRHGAAPQIEWREIPIDAINRPLDVIAAGDADVTIVDAPEFRFIAQAYPEVLSAFE
                     LERTRPVQWLVRRRSNALRAAIDAYLGTDEVAHQVAAAAVAPLEQSPRMQFEMARNFR
                     NHIETRLPLLRPWFEEAGRETGLDWRLLAAIGYQESQWDQDARSFNGALGVMMLMPPT
                     AESVGVMDPFDPRENIHGGARYFVEVRNKIPARIAEPDRTSMALASYNVGYGHLEDAR
                     VITQMRGGNPDKWEDVRRSLPLLAQEGWYSQVKRGYARGWEPVQFERRVRQYLSVLEW
                     QTLPSIETVLQAETEVLPPAEAATEVATQ"
     gene            5397..5489
                     /locus_tag="PKN91_09960"
     tRNA            5397..5489
                     /locus_tag="PKN91_09960"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5432..5434,aa:Ser,seq:cga)
     gene            complement(5492..7099)
                     /locus_tag="PKN91_09965"
     CDS             complement(5492..7099)
                     /locus_tag="PKN91_09965"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS28433.1"
                     /translation="MKHLAGSTAAPGTGASLRIDELLRAEAFPHPVAHIEVRETHISL
                     VILTGPFAYKIKKPVKFDFIDASTLERRRRLCEEELRLNRRYAAGLYLDVVPIVRLDG
                     RVAVGAAGPAIEYAVRMQQFDAASELPALLEANDVSQGEVLALAELLAGLHLHAPRAR
                     ESGVQANIRQPGEAVLENLSQLIGELARLEPPVAPGPIAEWAHDQARALEASFRLRED
                     SGYIRECHGDLHAANIVRWQGRLTPFDCIEFDPQLRWIDVINDLSFLVMDLVSRRRTD
                     LAFALLSRYLETTGDYGGVPLLRFYAVHRALVRAKVDALAAGQLPQRAGEFRERLLWR
                     IRAATDWMAPRRPVLILMHGPSGSGKSWLSERLVPALAAIRVRSDVERKRLAGIRPQQ
                     GAAAGLQQGIYSAEFSRRTYGRLADCAERCLQAGFNTIVDAAFLEPADRELFRDLAAR
                     LGAAYAIVACRGDPAMLAARIAERARAREDASDADLAILAAQLRALQPFPARERDLVT
                     TIDTSNPNAVEDVVAAIGTFGDRRHVR"
     gene            complement(7127..7597)
                     /locus_tag="PKN91_09970"
     CDS             complement(7127..7597)
                     /locus_tag="PKN91_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNS28434.1"
                     /translation="MSLIDCEPRSGETSMHSSQEEKPMAALSQEEIDRLNQLMEERRT
                     RELEEIRAVAARSNDDGRQELLAGRAADQLDQALGEIAQSATYAIVRQDIQDVRDIDA
                     ARKRIAAGTYGVCIECGVEIGFPRLLAYPTAKRCISCQREYERQQATREGRGAP"
     gene            complement(7704..>8278)
                     /locus_tag="PKN91_09975"
     CDS             complement(7704..>8278)
                     /locus_tag="PKN91_09975"
                     /inference="COORDINATES: protein motif:HMM:NF017987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ChuX/HutX family heme-like substrate-binding
                     protein"
                     /protein_id="HNS28435.1"
                     /translation="AGMGLPASASDFCATPEQTKQVRAFYATNPGTMPPVAATRLGLP
                     EATVVSALEPEQTASAAGSHFAEIWTAMGEWKEATFLIMKGANVFEIRSAVGKGTPST
                     KSQYYNIEYTHPLRGHLRLDLYASIYAVSLPVKDKVGPRGVLFYDAEGASVFGAFISG
                     DGPPPEAAELAKFDQLMALIRSKPAVCARE"
BASE COUNT         1313 a         2701 c         2812 g         1452 t
ORIGIN      
        1 atctgcgcga aaaagttctc cggattgcgg ttcagggtca tcgtgccgac cttgatcagc
       61 ggatagtccc tgtgcgacca ggtcttggtg agatcgaagg gattgaaccg gtagttcttt
      121 gcggcggcat agggcatcac ctgcaccgac atgacccagc tcgggaactc gctgcgcgcg
      181 atggcttcga acaggtcgcg gcgatggaag tcggcatcct ggccggccat cgcggccgcc
      241 tcggcattgc tgaagaactg catgccctgc tgggtatgga aatggtattt gacccagaac
      301 ttctcgccct gggcgttcac ccacatataa gtgtgcgatc cgtacccgtt catgtggcgc
      361 caggtgcgcg gcaggccacg ctggcccatc aggtaggtca cctggtgcgc gctctccggg
      421 ttactggtcc agaagtccca ctgcatgtgg ttgtcgcgca ggccgctgtc gggcaggcgc
      481 ttctggctgc ggatgaagtg cgggaacttc atggtgtcgc gcacgaagaa caccggcgtg
      541 ttgttgccga cgaggtcgta gtttccctca tcggtgtaga agcgcagcgc aaagccgcgc
      601 acgtcgcgcc aggtgtcggg gctgccggct tcgccggcga cggtcgagaa acgcgccagc
      661 atctcggtgg aggcacctcg ctggaacacg gccgccttgg tatagcgcga gacgtcctcg
      721 gtcgtcctga acacgccgaa cgcgcccgaa cccttggcgt gcggctggcg ctcgggcacc
      781 ttctcgcgat tgaagtgcgc catctgctcg aggaaatgca cgtcgtgcag gacgatcggg
      841 ccatcggcgc cgacggtgag ggaattgcgg tcgctcgctg cgggcgcgcc ggcaccggtg
      901 gtcgaggcgg cgagggatgg cttgctggac ttgctcatgg tgtacctccg gctggtcggg
      961 aagggcgaaa aatcagtcta aggccggggc aacggcaagt cgagatgccg ccgcctccat
     1021 gctaggattt ccacactgtg aacgcaccta cattcagcac tttctcggcc atcgccgaac
     1081 tcttcgtcac cgctggcgtg ttctacatcg tgttgcacaa ctacgcgcgc aaggggttcg
     1141 cctggcgcct cgccacggcc ctgatcgtgt tcgagttctc ggtcaacatg atgtacatga
     1201 tctttcgcat gcgcgaaacg acggccgggc acgaatccgc gggccccaat ccgctcgcgc
     1261 cgttcatggc cgcccacggc atcctgtcgt tgctggtgtt cctgctgctg gtcgtctatt
     1321 cgtatctcgc gttcgcggct tcccggcgcg gcaggttctt cttcgccgat catccggcgg
     1381 tggcctggtc gttcctcctg ctgtggacac tgtcggtcgg gtccggctgg gccctgtact
     1441 tcatcaacta cttctgatcg tcgcgtagag cagcgcgagt gcgatgaggc acatgccgat
     1501 gagcgcatgg cgcacggccg cgcgcgcggc cgtcgcgtcc ggttcctgcg cgagcgtgta
     1561 ggcgcccacc agcgccaggt gcgcgctcac cagcagcacg aacacgaagc cgaagaatat
     1621 gtgcgcgagg tccaccatcg tgtagaggcg cgtaccggcc acgagcggtg tcacgagtgg
     1681 caggccggtg agcagcgtgc cgcagatcat gaagaaggcg tgcacgccga cccggtaggg
     1741 tgcgcgcatc acgaacgggc gcacgcgcgc gtagtgacgc agctggtaga gcgcgtaggg
     1801 cgcgatcgtg gcgagcgcgc cggtccagtg cgcgaagacc cagaagtcgc gccggatcgg
     1861 ggacatcggc atgaacagca gcgtcgcacc tgacagcagc gcgaacagca gcccccccag
     1921 cgtcacgagg agcagtggtg agcgccagcg ctgcgtggcg cgcgactttt gcggggtgaa
     1981 caggggcccc gccgcatgca gcttcatgcg aacgagcatg ccacaggacg atacaggggc
     2041 ggatacgcga atgccgccgg gtatcatgga tgcatgagca gcacacctca ccgcacggat
     2101 gtcgacttca tgcggcgcgc gctcgtgctc gccggcgagg ccgaatcgca tggtgaggtg
     2161 ccggtcggcg ccgtgctcgt gcgcggcgag gagatcgtcg gcgaaggatt caaccgcccg
     2221 atcggtggtg tcgacccgac ggcccatgcg gagatcgaag cgctgcgcgc cggcgcgcag
     2281 gcgctcggca gctaccggct caccggcacc acgctctatg tgacgctcga gccctgcctg
     2341 atgtgtgccg ccgcgatcgt gcacgcgcgg gtgcgacgag tggtgttcgg tgcctgggat
     2401 ccgaaggcgg gtgccgccgg cagcatcatc gacgcgttcg ccctgccggc catgaatcat
     2461 cgcgtcgaca cattcggcgg cgtgcttgcg gaggagtgcg gcgagctgct gcggcgcttt
     2521 ttcgccgcga ggcgggggcc atgagcgcgt tgcgcagcgt ttgcgtctat tgcggctcgc
     2581 aaaagggcgc gtcgcccgca ttcgcgcagg ccgcggccga tctcgggacg gcgctcgcgc
     2641 gcgaggggct gacgctggtc tatggcggcg caagcgtcgg gctgatgggc gtcgtggccg
     2701 atgccgcgct cgccgcgggc ggtcgcgtga tcggcgtgat cccgcgcatc ctgatgcgca
     2761 aggagctggc gcacgccgga gtcaccgaac tcgtggtgac cgaatcgatg catgagcgca
     2821 aggcgcagat ggccgcgcgc tcggacgcgt tcatcgcgct ccccggcagc atcggtacgc
     2881 tcgacgaatt gttcgagatg tggacctggg cgcagctcgg cctgcacgcc aagccctgcg
     2941 tgctcgtcaa cacggacggt tactacgatg gcctgatcga gttcctgggg cgcgcggagc
     3001 aggaaggttt cctgcgtccc acgacccggg cgctgttgcg aatcgttcgc gaattaccct
     3061 cgcctcgtgc tgtcctgaac ccctgatcag tgtcgacctg ctttacaggc ggcggccatg
     3121 gtgcgtcgcg aaattcagta actgattgat ttcgaatcat tgtttagatt tggcccgcaa
     3181 cttgctccat aagggccatc tgacatggga gttgcgggaa aatgataaag aagcttcgct
     3241 tggcgttcgc cggtcttgcc ctgatcggtt caggaatcgc ccactccgcg atcatcgatc
     3301 tcggcagtgt cacagcgccg ggcgcgctga ccgtcgccaa cagctggggc gtggcccccg
     3361 ggactttcac cgatcagcac ctgttcacgc tcaccaacgc cgccgactct tacggcggga
     3421 tgatcgagct cgactggttc ttcggcaata cgaacgtcta ctccgttgcg ctcgtcgggc
     3481 tgaatggcac gagctacttc ggtctcgacc tggatccggt gtcgacattg tgggagccga
     3541 tctcgctcgc gttcagtggt ctcagcgccg gcgcttactc gctggtggtg tcgggcgctg
     3601 catacggatt cgcgaccggc tacgaggggg tgctcgcctt ctggcccgcc agttcggcca
     3661 gcgtcccgga gccgagcacg ctcgcgctgc tcggattcgg cctgattgcg ctcggcgtca
     3721 tgcgccgtcg cctctctgcc tgatcgcagt cgacaacaca tcgtccgacg aaaccccggc
     3781 ctcgcgccgg ggttttcttt tggggcccgc tattgcgtcg cgacttcggt tgcggcctct
     3841 gcgggaggca gcacttcggt ctccgcctgc agcaccgttt cgatcgatgg cagcgtctgc
     3901 cactcgagca cgctcaggta ttgccgcacg cgccgctcga actgcaccgg ctcccagccc
     3961 cgcgcgtaac cgcgcttcac ctgcgagtac cagccttcct gcgcgagcag cggcaggctg
     4021 cggcgcacgt cctcccactt gtccggattg ccgccgcgca tctgcgtgat gacgcgcgca
     4081 tcttcgaggt gtccgtagcc gacgttgtac gaggcgagtg ccatcgaggt gcggtcgggc
     4141 tcggcgatgc gagccggtat cttattgcgc acctcgacga aatagcgcgc gccgccgtgg
     4201 atgttctcgc gggggtcgaa gggatccatc acgccgaccg attccgccgt gggcggcatc
     4261 agcatcatga cgccgagcgc accgttgaag gagcgcgcgt cctggtccca ctgcgattcc
     4321 tggtagccga tcgcggcgag caggcgccag tcgagccccg tctcccggcc cgcttcctcg
     4381 aaccacggcc gcagcagcgg caggcgggtt tcgatgtgat tgcggaaatt gcgcgccatc
     4441 tcgaactgca tgcggggcga ctgctcgagc ggggcgaccg ctgctgcagc gacctggtgc
     4501 gcgacctcgt ccgtgccgag ataggcgtcg atcgcagcgc gcagcgcatt cgagcgcctg
     4561 cgcaccagcc actgcaccgg acgcgtgcgt tcgagctcga aggccgacag cacctccgga
     4621 tacgcctggg cgatgaaacg gaactcgggc gcatcgacga tcgtcacgtc ggcgtcgcct
     4681 gccgcgatca cgtcgagcgg tcgattgatc gcatcgatcg ggatctcgcg ccactcgatc
     4741 tgcggtgccg cgccgtgacg cagcgcctcg agcacgccga cctgcgcgct ctcggccttg
     4801 acgacgatgc ggcgaccgac gagttccctg gttttcccgg gcttgaccgt gccgcgcgcg
     4861 tagacgacga gctggacgac ctggtcgtag ttgcgcgagg gcagggcgaa caggcgccag
     4921 cggtcccgat aggtcagctg cgcggccgcg atgtcgacgg cacccgagcg cagcgcctcg
     4981 agcagttcgc tctcgctggc gaagggtgtc atggcaaggc gtacgcccag cgaatcggcg
     5041 aatccgcgcg cgagctggta ctcgatgcct tccggcccct gggcgccgaa ataataggtc
     5101 gtcggcaggt tcagggtgcc cacgcgcagt tcgctgcggg cccggatctg ctcaaggttc
     5161 gacggcgggg cggagcactg cgcaaggccc gcaagggccg tcagcacgcc gagggtccgc
     5221 caccaggacg gaccgcggaa gcggcgcatc aagccacctc acaggctctg ggaacttcgg
     5281 ctagaatagc aactccagcg tgcctgtcgc gacccgcgaa agcggtgtaa tttcaatgcc
     5341 aaatcaagta tttgggcttg aaatcagcac ccgtttcgca tactagaggc acacccggag
     5401 aggtaccgaa gtggtcataa cggcgccgac tcgaaatcgg atggtcgggt aattccggca
     5461 cgtgggttcg aatcccaccc tctccgccac ttcaacgaac gtgccgccgg tcgccgaagg
     5521 tgccgatcgc ggcgacaacg tcttcgacgg cattcgggtt gctcgtgtcg atcgtcgtga
     5581 cgaggtcccg ttcacgtgcc ggaaaaggct ggagggcgcg aagctgggcc gcgaggatcg
     5641 cgaggtcggc atcggaagca tcctcgcgcg cgcgtgcccg ctcggctatc cgggcggcca
     5701 gcatcgccgg atcgccccgg caagcgacga tggcataggc cgccccgagc ctcgcggcga
     5761 gatcccggaa cagctcgcga tcggccggct cgagaaacgc cgcatcgacg atggtattga
     5821 accccgcctg caggcatcgt tccgcgcaat cggccagccg cccgtatgtc ctgcggctga
     5881 actcggccga gtagatcccc tgctgcagcc ctgcggcggc gccttgctgc ggccggattc
     5941 cggcgagacg cttgcgttcg acatcggagc gcacgcgaat cgcggcgagc gccgggacca
     6001 ggcgctcgct cagccaggac ttgcccgagc ccgacgggcc gtgcatcagg atgagcaccg
     6061 ggcgccgggg cgccatccag tcggtggcgg cgcgaatgcg ccagagcagg cgctcgcgga
     6121 actcgccggc acgctgcggc aactgcccgg cagccagcgc gtccactttc gcgcgcacga
     6181 gcgcgcgatg taccgcgtag aagcgcagca gcggcacgcc accgtagtct ccggtggtct
     6241 cgaggtaccg gctcagcagg gcgaatgcga ggtcggtacg gcgccggctc acgagatcca
     6301 tgaccaggaa ggagaggtcg ttgatcacgt cgatccagcg tagttgcggg tcgaattcga
     6361 tgcagtcgaa cggggtgaga cgaccctgcc agcgcacgat attcgcggcg tgcaggtcgc
     6421 cgtggcactc gcggatgtag cccgagtcct cgcgcagccg gaaggatgcc tccagggccc
     6481 gggcttggtc gtgcgcccat tccgcgatcg ggccgggcgc gacaggcggt tcgagccgcg
     6541 cgagttcgcc gatcaactgg gagaggtttt cgagcaccgc ctcgcctggc tggcggatgt
     6601 tcgcctgcac gcccgactcg cgcgccctcg gcgcgtgcag atgaaggccg gccagcaact
     6661 ccgccagcgc gagaacttca ccctgggaga cgtcgttcgc ctcgagcagt gcgggcagct
     6721 cgctcgccgc gtcgaactgc tgcatgcgca cggcatattc gatggcgggt cccgctgcgc
     6781 cgaccgcgac acgaccgtcg aggcgaacga tcgggaccac atcgaggtag agcccggcag
     6841 cgtagcggcg gttgagtcgc agttcctctt cgcacagccg gcgtcgccgc tcgagtgtgg
     6901 aggcatcgat gaagtcgaac ttcaccggct tttttatctt gtacgcgaac ggtcccgtga
     6961 ggatcaccag ggagatgtgt gtctcgcgca cctcgatgtg cgcgaccggg tggggaaatg
     7021 cctccgcgcg caggagctca tcgatacgca gggaggcccc ggtcccggga gcggcggtac
     7081 tgcctgcgag gtgtttcatg cggagtccga cagtgtcgtg tgccggttac ggggcgccgc
     7141 gtccctcccg ggtcgcttgc tgccgttcgt attcccgctg gcagctgatg cagcgcttgg
     7201 cggtcgggta tgcgaggagg cgcgggaagc cgatctcgac gccgcattcg atgcagaccc
     7261 cgtacgtgcc ggcggcgata cgcttgcgag ccgcatcgat gtcgcgcacg tcctggatat
     7321 cctggcgaac gatcgcgtag gtggcgctct gcgcgatctc gcccagcgcc tggtccagct
     7381 ggtcggcggc ccggccggcg agcagctcct gcctgccatc gtcgttggat cgcgccgcca
     7441 ccgcgcggat ttcttccagt tcgcgcgtcc ggcgctcctc catgagctgg ttcagacgat
     7501 cgatctcttc ctgcgacagg gccgccatcg gcttctcctc ctgcgacgag tgcatggaag
     7561 tctcgcccga tcgtggctcg cagtcgatca gggacatcca cgtgcatgcg accgtgtttc
     7621 ccgcgccgcg ccggcgcgcg gtttgtcgag cccctgccgc tgtcgccggg cgtcactggc
     7681 gccagacaaa agttggacgg cgtctattcc cgcgcgcaga cggcaggctt cgagcgtatc
     7741 agcgccatca gttgatcgaa tttcgcgagt tcggcggcct cgggcggcgg accgtcgccc
     7801 gagatgaagg cgccgaacac cgacgcgccc tccgcgtcgt aaaacaacac gccgcggggg
     7861 cccaccttgt ccttgaccgg cagcgatacg gcgtagatcg acgcatagag atcgaggcgc
     7921 aggtggccgc gcaacggatg cgtgtactcg atgttgtagt actggctctt cgtcgagggc
     7981 gtgcccttgc caacggcgct cctgatctcg aacacattgg cgcccttcat gatcaggaaa
     8041 gtcgcctcct tccactcgcc catggcggtc cagatttcgg cgaagtggct gcccgcggcg
     8101 gacgccgtct gttccggctc gagcgcgctc acgacggtcg cctccggcag gccaaggcgg
     8161 gtggctgcca ccggcggcat ggtgccggga ttcgtcgcgt agaaggcgcg cacctgcttc
     8221 gtctgctcgg gcgtcgcgca gaagtcactg gccgacgcgg gcagtcccat gccggcga
//
LOCUS       DAOQVY010000110         8042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_9914_length_8042_cov_5.573432, whole genome shotgun sequence.
ACCESSION   DAOQVY010000110 DAOQVY010000000
VERSION     DAOQVY010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9914_length_8042_cov_5.573432"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2140
                     /gene="rbbA"
                     /locus_tag="PKN91_09980"
     CDS             <1..2140
                     /gene="rbbA"
                     /locus_tag="PKN91_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HNS28436.1"
                     /translation="TAYMEEAARFDWLCAMDGGRVLATGSPAELLARSGRSSLEAAFI
                     DLLPEARRAGHTPVRVVPREPGTGDSIAIEAQDLTMRFGEFTAVDHVSFRIRRGEIFG
                     FLGSNGCGKTTTMKMLTGLLPASEGRAWLFGQPVDPHDLATRRRVGYMTQAFSLYGEL
                     TVRQNLELHGRLFSLPEARVQARIEALAGRFGLRTLLDDLPGKLPLGQRQRLSLAVAL
                     IHEPELLILDEPTSGVDPVARDAFWRELIALAREDGVTIFISTHFMSEAERCDRISFM
                     HAGKVLISDTPTALVARYGVHTLEEAFVACLQETIGEPAQHTLPAMAAPHASVGKGGG
                     WRRHFDPRRMLAYLGREARELRRDRIRLTLAGLGSAILMFVLGYGISMDIENLSFAVL
                     DRDDTSLSRDYADTLAGSRYFTRQPPASDEADIDRRMREGRLSLALEIPEGFARDVAR
                     GEPVQIAAWIDGAMPQRAQTVMGYVQGMHQHWLLAHARETLGDAALAADFRIETRFLY
                     NPSIRSLNAIVPAMIPLLLLLIPAILSALAVVREKELGSIVNLYVTPVTRLEFLLGKQ
                     LPYIAVAMANFLLMTLLAVTVFGVPLKGSFATLAAAAGLYVVSATALGLFISAFVRSQ
                     VAALFGTSMLTILPAVQFSGLLNPVSSLEGFAAMIGHAWPTTHFITIARGTFAKGLGF
                     QELQAAFLPLLIAIPVIVGLATLALRKQER"
     gene            2142..3266
                     /locus_tag="PKN91_09985"
     CDS             2142..3266
                     /locus_tag="PKN91_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS28437.1"
                     /translation="MRGERNFLHLTAKELRSLLRDPMLLALIVYAFSGAIYIAATAMP
                     EPLNSAAIAVIDEDATPLSARMADAFYPPRFLPPRFITTTEMDARMDAGLDTFALHIP
                     AGFQRDLLAGRSPGLQLNVDATRMSQAYIGSAYIQSIVTGELQEYLERERRLTSPPVD
                     LALRARFNPELEDRWFGSVMELISNITMLSIILTGAALIREREHGTIEHLLVMPVTPM
                     QIMGSKVLAMALVVLLASSLSLLVVIQGWLRVPIEGSISLFLAGAALLLFATTSMGIF
                     LATIARSMPQFGLLMLLVLMPLEILSGGMTPPESMPDLVRWIMLGAPTTHFVALAQAI
                     LYRGAGFAIVWPQFLALLVIGALLFTASLAWFRRALARMA"
     gene            complement(3296..4594)
                     /locus_tag="PKN91_09990"
     CDS             complement(3296..4594)
                     /locus_tag="PKN91_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNS28438.1"
                     /translation="MARRIQEVLLIETRHRPGSLAAALQVLAHHGINLEHLHALSRER
                     GWTRWELTVEVTPAMLEGALKALDGLADARYVGRSDRVFQRHRGGKIAVRPTLAISTQ
                     QILRDVYTPGVAVVSTAIAEQPELARDYTIKGQSVAVISNGTAVLGLGAVGPLASLPV
                     IEGKAALLASLVDVVAMPLAVEGRDVDALVAAIAAFEPSFGAILLEDFSAPDCFELES
                     RLRARLPIPVMHDDQHGTAVVVLATLLRICRRYGRELHSLTVGQVGLGAAGLGICSLL
                     ARYGCRSLRGTDIKPEALERLRAIGGTPDTLEGVMQRCDAVVLTSGVPGLVEPAMIRP
                     GQIVLSLSNPDPEIDPIAAQDAGAAFAADGKIINNVLGFPGIFRGALDARASAITDAM
                     LFAAAEALADLAPEDDVAPNPLDREVHAAVAARVRAAAGT"
     gene            complement(4643..5434)
                     /locus_tag="PKN91_09995"
     CDS             complement(4643..5434)
                     /locus_tag="PKN91_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNS28439.1"
                     /translation="MTTDDGRAPLSAADIARFGPDPFATFRANLATVEQRIARACARA
                     GRDRASVRLLPVTKTVPAHVLRYAHAAGIRNFGENKIQEASAKHEALADLAIEWSIVG
                     HLQTNKAKYLVRFAHEFQALDSLRLAEILDQRLASENRSLDVYVQVNTSGEASKFGLQ
                     PDALLPFVDALAQFPRLRPRGLMTLALFSADMARVRPCFVLLRTLRDAALARHPGIVG
                     LSMGMSGDFEAAIEEGATVVRVGQAIFGERPTPDSHYWPGAGAPS"
     gene            complement(5449..6282)
                     /locus_tag="PKN91_10000"
     CDS             complement(5449..6282)
                     /locus_tag="PKN91_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNS28440.1"
                     /translation="MKSITLLLCFGLLSASAHALDLQLEGRRALVTGSTSGIGYSIAR
                     ALLAEGADVIVNGRTEDGVKAAIEKLREETGRTARGFAADMSTPGGANAIHAAFPDVE
                     ILVNNVGAYGAKPFEQTTDEDWYRLIELNVMSGVRLSRLYLPGMRQRNWGRIIFISSE
                     SAYHIPPDSIQYGMTKAAQIAVARGLAEATAGTRITVNSVLPGPTRARPESGKAADGR
                     LWEEVERRFFTERRPTSLLKRFGHPDEIGHFVAFIASPLASATTGAALRADGGTVKSA
                     F"
     gene            complement(6412..7653)
                     /locus_tag="PKN91_10005"
     CDS             complement(6412..7653)
                     /locus_tag="PKN91_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28441.1"
                     /translation="MKSRALYLLSFAALAACASYVHADEQQQSVDAALKATVPLVDVR
                     LRYENVDQDGLPENADALTLRARLGFETGKAWNTSMLVEGNFMLPLQNDYRADPAVAR
                     NVQYPVVADPENYAINRLQLINTSLPQTTITLGRQRINLDDQRFIGNVGWRQNEQTYD
                     ALRVVNKSVKNLTLDVTWMNQVNRVFGKDSPQGRYSGDSLLANVGYRLPVGVLTGFGY
                     LLSFDPMGTVPAAQDSSATWGLRFNGERALQNSVKLTYAASYANQRERGANPLRFEND
                     YYLAEFGAAYRQFNVGLGWEILDGDGSKGFATPLATLHKFQGWADKFLATPVNGIDDR
                     YASAGFVTKSAGPFDSLALLASHHRFRSQRLSQAYGSETGLQVQVKRHRFTGMLKFAD
                     YDASGFATDTSKLWVQVEYAR"
     gene            complement(7831..>8042)
                     /locus_tag="PKN91_10010"
     CDS             complement(7831..>8042)
                     /locus_tag="PKN91_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28442.1"
                     /translation="GSLANQEMRSSRNSGTQQKTRVLSLANQEMRSSRNMSVGEVSSG
                     RELSKSGNALQPERRDVLQRCDAGA"
BASE COUNT         1237 a         2704 c         2707 g         1394 t
ORIGIN      
        1 caccgcctac atggaggagg cggcacgctt cgactggctc tgcgccatgg atggcgggcg
       61 cgtgctcgct accggttcgc ccgcggagct gctcgcccgc agcggtcgca gcagcctcga
      121 agccgcattc atcgacctgc tgccggaggc ccgccgcgcc ggccacacgc cggtgcgcgt
      181 cgtgccgcgg gagccgggta ccggggattc catcgccatc gaagcgcagg acctcacgat
      241 gcgcttcggc gagttcacgg cggtcgacca cgtgagcttt cgcatccggc gcggagagat
      301 cttcggcttc ctcggatcga acggctgcgg caagactacg accatgaaga tgctgaccgg
      361 cttgttgccg gccagcgagg ggcgcgcctg gctcttcggc cagcccgtcg atccgcatga
      421 tctcgcgacg cgccggcgcg tcggctacat gacccaggcc ttctcgctct atggcgagct
      481 gaccgtgcgc cagaatctcg agctgcacgg gcggctcttc agcctgccgg aggcgcgggt
      541 gcaggcgcgc atcgaggccc tggctggccg cttcggactg cgcacactgc tcgacgatct
      601 gccgggcaag ttgccgctcg gccagcgaca gcggctgtcg ctggcggttg cgctgatcca
      661 cgagccggag ctgctgatcc tcgacgaacc gacctccggg gtggatccgg tcgcccgcga
      721 cgcgttctgg cgcgagctga tcgccctcgc gcgcgaggac ggggtgacga tcttcatctc
      781 cacccacttc atgagcgagg ccgagcgctg cgatcgcatc tctttcatgc atgccggcaa
      841 ggtactgatc agcgatacgc cgacggcact ggtcgcgcgc tacggcgtgc acacgctcga
      901 ggaggccttc gtcgcctgcc tgcaggagac gatcggcgag ccggcgcaac acacactgcc
      961 ggcgatggcg gcgccacacg cttcggttgg caagggcggc ggctggcggc ggcacttcga
     1021 cccccgtcgc atgctcgcct acctgggccg cgaggcgcgc gagctgcgcc gcgaccgcat
     1081 ccggctgacc ctggccggac tcggcagcgc gatcctgatg ttcgtgctcg gctacggcat
     1141 cagcatggac atcgagaacc tgagcttcgc ggtgctcgat cgcgacgaca cctcactgag
     1201 tcgtgactat gccgacacgc ttgccggctc gcgctacttc acacgacagc cgccggcgag
     1261 cgacgaggcg gacatcgatc gccggatgcg cgaggggcgg ctgagcctcg cactggaaat
     1321 cccggagggc ttcgcgcgcg acgtggcgcg gggcgagccg gtgcagatcg ccgcctggat
     1381 cgacggcgcg atgccgcagc gggcgcagac ggtcatgggt tatgtgcagg gcatgcacca
     1441 gcactggctg ctcgcgcatg cgcgcgagac gctcggcgac gcggcgctcg cggcggattt
     1501 ccgcatcgag acccgattcc tctacaaccc cagtatccgc agcctcaatg ccatcgtgcc
     1561 cgcgatgatt ccgctcctgc tgctgttgat tccggccatc ctctcggcgc ttgcggtggt
     1621 gcgcgagaag gagctcggct ccatcgtcaa tctctacgtc acgccggtca cccgcctgga
     1681 attcctgctg ggcaagcagc tgccgtacat cgccgtggcg atggcgaatt tcctgctgat
     1741 gacgctgctg gccgtcaccg tgttcggtgt gccgctgaag ggcagcttcg cgacactcgc
     1801 ggcggcggcg gggctctacg tcgtctcggc gactgcgctc gggctgttca tttccgcctt
     1861 cgtgcgcagt caggtggcgg cgctgttcgg cacctcgatg ctgaccatcc tgccggcggt
     1921 gcagttctcc gggctcctga acccggtgtc gtcgctggaa ggtttcgcgg ccatgatcgg
     1981 ccatgcctgg ccgacgacac atttcatcac gatcgcccgc ggcaccttcg ccaagggtct
     2041 cggcttccag gaactgcagg ccgccttcct gccactgctg atcgccatcc ccgtgatcgt
     2101 cgggctggcc acgctcgcgc tgcgcaagca ggagcgctga gatgcgcggc gaacgcaact
     2161 ttctgcacct gaccgccaag gagctgcgca gcctgctgcg cgatccgatg ctgctggcgc
     2221 tcatcgtgta tgcgttcagc ggcgccatct atatcgcggc gacggcgatg ccggagccgc
     2281 tgaacagcgc ggccattgca gtcatcgacg aggacgccac gccgctctcg gcccgcatgg
     2341 ccgatgcctt ctacccgccg cgcttccttc cgccccggtt catcaccacc accgagatgg
     2401 acgcccgcat ggacgcgggc ctcgatacct tcgcactgca catccccgcg ggtttccagc
     2461 gcgacctgct ggccgggcga tctccggggc tgcagctgaa tgtcgacgcg acgcgcatga
     2521 gccaggccta catcggcagc gcctacatcc agagcatcgt caccggcgag ttgcaggaat
     2581 atctcgagcg cgagcggcgc ctcacgagcc cgccggtcga tcttgcgctg cgcgcccgct
     2641 tcaatccgga gctggaagat cgctggttcg gatcggtgat ggagctgatc agcaacatca
     2701 ccatgctgtc gatcatcctc acgggcgccg cgctgatccg cgagcgcgag cacggcacga
     2761 tcgaacacct gctggtgatg ccggtgaccc cgatgcagat catgggcagc aaggtgctgg
     2821 ccatggctct cgtggtgctg ctggcatcaa gcctgtcgct gctggtggta atccagggct
     2881 ggctgcgggt gccgatcgag ggctccatca gcctgttcct cgcgggtgcc gcgctgctgc
     2941 tgttcgccac cacctcgatg ggcattttcc tcgcaaccat cgcccgttcc atgccgcagt
     3001 tcggcctgct gatgctgctg gtgctgatgc cgctcgagat cctctccggc ggcatgacgc
     3061 cgcccgagag catgcctgac ctggttcgct ggatcatgct cggcgcgccc accacgcact
     3121 tcgtggcgct ggcgcaggcc atcctctatc gcggtgcagg cttcgcgatc gtctggccgc
     3181 agttcctggc cctgctcgtg atcggcgcgc tgctgttcac ggcttcgctc gcctggtttc
     3241 gccgggccct ggcccgtatg gcctgaggag gccaggccgc actcgcagtc gggatctacg
     3301 tccccgcggc cgcacgcacg cgcgcggcca ccgcggcatg cacctcgcgg tcgagcggat
     3361 tgggtgcgac atcgtcctcg ggcgcgagat ccgcgagcgc ctcggccgct gcgaacagca
     3421 tggcatcggt gatcgcgctc gcccgcgcat cgagagcgcc gcgaaagatc cccgggaatc
     3481 cgaggacgtt gttgatgatc ttgccgtcgg ccgcgaacgc cgcgccggca tcctgcgcgg
     3541 cgatcgggtc gatttccgga tcgggattcg acaggctcag cacgatctgc cccggacgga
     3601 tcatcgcggg ttcgaccaga cccggcacgc cgctcgtcag cacgacggcg tcgcagcgct
     3661 gcatcacacc ctcgagcgtg tcgggggtgc cgccgatggc gcgcaggcgt tcgagtgcct
     3721 cgggcttgat gtccgtgccg cgcaacgagc ggcagccgta gcgcgcaagc agcgagcaga
     3781 tgccgagccc ggcggcgccg agcccgacct ggcccaccgt gagcgagtgc agctcgcgtc
     3841 cgtagcgccg gcagatgcgc agcagtgtcg cgagcacgac gacggcagtg ccgtgctggt
     3901 cgtcgtgcat gacgggaatc ggcagccgcg cgcgcaggcg cgactcgagt tcgaaacagt
     3961 cgggcgcgga aaaatcctcc agcaggatgg cgccgaacga cggctcgaac gccgcaatgg
     4021 ccgcgacgag cgcatcgacg tcgcgtccct cgacggcaag cggcatcgca acgacatcga
     4081 cgagactggc gagcagcgcg gcctttcctt cgatgaccgg cagcgaggcg agcgggccga
     4141 cggcaccgag gccgagcaca gccgtgccgt tgctgatgac ggcgacgctc tggcccttga
     4201 tggtgtagtc acgcgcaagc tcgggctgct ccgcgatggc ggtcgagacg accgcgacac
     4261 cgggcgtgta gacatcgcgc aggatctgct gggtggagat cgcgagagtg ggccgcacgg
     4321 cgatcttgcc gccccgatga cgctggaaga cgcggtcgga gcgtcccacg tagcgcgcat
     4381 cggcgaggcc gtcgagcgct ttgagtgcgc cctcgagcat cgccggcgtc acctcgaccg
     4441 tgagctccca gcgtgtccag ccgcgctcgc gcgaaagcgc atgcaggtgc tcgaggttga
     4501 tgccgtggtg cgcgagtacc tgcaacgcgg ccgccagcga acccgggcgg tgccgggtct
     4561 cgatgaggag gacttcctgg atccttcgtg ccatttataa atggtaaacg aaagaaaccc
     4621 gcgcggtgcg ggcggcagtg cgtcaggacg gcgcaccggc cccgggccag tagtggctgt
     4681 ccggcgttgg gcgctcgccg aaaatggcct gtccgacgcg cacgaccgtc gctccttcct
     4741 cgatcgccgc ttcgaagtcg cccgacatgc ccatcgagag cccgacgatg ccgggatggc
     4801 gcgcgagggc ggcgtcgcgc aacgtgcgca ggaggacgaa gcagggccgg acccgcgcca
     4861 tgtcggcgct gaacagtgcc aacgtcatca gcccgcgtgg tctcaggcgc gggaactgcg
     4921 cgagcgcatc gacgaacggc agcagcgcat ccggttgcag gccgaacttg ctcgcctcgc
     4981 cggaggtgtt gacctgcacg tagacatcga gcgagcggtt ctcgctcgcg agccgctggt
     5041 cgaggatctc cgcgagccgc aggctgtcga gcgcctggaa ctcgtgcgcg aagcggacga
     5101 ggtacttggc cttgttggtc tgcaggtgcc cgacgatgct ccactcgatc gcgagatccg
     5161 ccagcgcctc gtgcttcgca ctcgcctcct ggatcttgtt ctcgccgaaa ttcctgatgc
     5221 cggccgcgtg cgcgtagcgc aggacgtggg ccggcacggt cttggtcacc ggcagcagcc
     5281 gcacggaggc gcgatcgcgc cccgcccgtg cgcaggcgcg ggcgatgcgc tgctccaccg
     5341 tcgccagatt ggcgcggaac gtcgcgaacg gatcggggcc gaagcgcgcg atatccgcag
     5401 cggagagcgg tgcgcggccg tcatcggtgg tcatccgcag ccctgcgcct agaacgcgct
     5461 cttgaccgtg ccgccgtcgg cgcgcagagc cgcgcccgtc gtggcggatg ccagcgggct
     5521 ggcgatgaag gcgacgaagt ggccgatttc gtcggggtgc ccgaagcgct tgaggagcga
     5581 cgtcgggcgc cgctcggtaa agaaccgccg ttcgacttct tcccacagcc gcccgtccgc
     5641 ggccttgccg ctctccggcc gcgcccgcgt cggtccgggc agtacgctgt tcacggtgat
     5701 ccgggtgcct gcggtggctt cggcgagccc gcgcgccacg gcgatctgcg cggccttggt
     5761 catgccgtac tggatgctgt cgggcgggat gtggtaggcg ctctcgctcg atatgaaaat
     5821 gatgcgtccc cagttgcgct ggcgcatgcc gggcaggtaa aggcgcgaga gccgcacgcc
     5881 gctcatcaca ttgagctcga tcagccggta ccagtcctcg tccgtggtct gctcgaaggg
     5941 tttcgcacca taggcgccga cattgttcac gaggatttcg acgtccggga aggccgcatg
     6001 gatcgcgttc gcgcccccgg gcgtcgacat gtccgcggcg aatccgcgcg cagtgcggcc
     6061 ggtttcctcg cgcagctttt cgatcgccgc tttcacgccg tcctcggtac gcccgttgac
     6121 gatgacgtcg gcgccttcag ccagcagcgc cctggcgatc gagtagccga taccggaggt
     6181 actccccgtg accagcgcgc gccggccttc cagttgcagg tcgagtgcgt gggcgctggc
     6241 ggacagcagg ccgaagcaaa gcagcagcgt gatggatttc atggcgactc ccggatgacg
     6301 cgcgcgttgg cgcgcatgcg ccaacgatac ccaactcgca gtcagccggg tgggtgtggt
     6361 tcgcccgatg agcctgccgg ggcgcggcgc cccggcagtg gcgggccggt ctcagcgcgc
     6421 gtattccacc tgcacccaga gcttgctcgt gtcggttgcg aagcccgagg catcgtagtc
     6481 tgcgaacttg agcatgccgg tgaagcggtg gcgcttgacc tgcacctgca ggcccgtttc
     6541 ggagccgtag gcctgcgaca ggcgttgcga gcgaaaccgg tgatggctcg cgagcagcgc
     6601 gaggctgtcg aacgggccgg ccgatttcgt gacaaagccc gcactcgcgt agcggtcgtc
     6661 gatgccgttc accggcgtcg cgaggaactt gtcggcccag ccctggaact tgtgcagcgt
     6721 ggcgagcggg gtcgcaaaac ccttgctgcc gtcaccgtcg agaatctccc agcccaatcc
     6781 cacgttgaac tgccgatacg cggcgccgaa ctcggcgaga tagtagtcgt tctcgaagcg
     6841 cagagggttc gcgccgcgct cacgctgatt cgcataggag gccgcgtagg tcagcttgac
     6901 ggagttctgc agggcgcgtt cgccattgaa ccgcaggccc caggtcgcgg atgaatcctg
     6961 cgcggcggga accgttccca tgggatcgaa ggagagcagg tagccgaagc ccgtcagcac
     7021 accgacaggc aggcgatatc ccacattcgc gagcaggctg tcgccactgt aacgaccctg
     7081 cggggaatcc ttgccgaaca cgcggttgac ctggttcatc caggtcacgt ccagcgtgag
     7141 gttcttgacg ctcttgttca cgacgcgtaa tgcatcgtaa gtctgctcgt tctggcgcca
     7201 gcccacattg ccgatgaagc gctggtcgtc caggttgatc cgctgccggc ccagggtgat
     7261 cgtcgtctgc ggcagcgagg tgttgatcag ctgcaggcgg ttgatcgcgt agttctccgg
     7321 atcggcaacc accggatact gcacgttgcg tgcgacggcc ggatcggccc gatagtcgtt
     7381 ctgcagcggc agcatgaaat tgccctcgac cagcatcgag gtgttccagg ccttgccggt
     7441 ctcgaagccc agcctcgcgc gcagcgtcag ggcgtcagcg ttctcgggaa gcccgtcctg
     7501 gtcgacgttc tcgtagcgca ggcgcacgtc caccagtggc accgtggcct tcagcgccgc
     7561 atccacggat tgctgctgct catctgcatg cacgtacgat gcgcacgcag ccagcgccgc
     7621 gaagctcaaa aggtacagtg ctctcgactt catgtaggga cctccagagg ctatctgcaa
     7681 tcagacaccc gaaccgcgga atgcgacctg atgtatgtca atcggaggca acgaataggc
     7741 cggtcggggt gcaggcgacg cgatggggtg cgatcgtgtc cgggccgacg tggcggaata
     7801 cgttccggct ggagcgcatt tcctgatttg ctaagctcct gcatcacatc gttgtagtac
     7861 atctcgtcgt tccggctgga gcgcatttcc tgatttgcta agctccctgc cggagctcac
     7921 ctcgccgacg ctcatgttcc ggctggagcg catttcctga tttgctaagc tcaaaactcg
     7981 tgttttttgt tgcgtgcctg agttccggct ggagcgcatt tcctgatttg ctaagctccc
     8041 at
//
LOCUS       DAOQVY010000111         7867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_10196_length_7867_cov_5.838454, whole genome shotgun sequence.
ACCESSION   DAOQVY010000111 DAOQVY010000000
VERSION     DAOQVY010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7867
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10196_length_7867_cov_5.838454"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..287
                     /locus_tag="PKN91_10015"
     CDS             54..287
                     /locus_tag="PKN91_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HNS28443.1"
                     /translation="MTDTWLSTLITDTPQAGFELAITLSRRGVKYTQPDTAVLKKLRP
                     AYADSADGLTAASQVIAINFQTVAAANNYWRTA"
     gene            305..1249
                     /locus_tag="PKN91_10020"
     CDS             305..1249
                     /locus_tag="PKN91_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HNS28444.1"
                     /translation="MRYATVSASILDAIGDTPLLDIDGVFAKCEFVNPSGSIKARIAH
                     YIVERAEKAGLLKPGDTIVEATSGNTGNAFSMVAAVKGYRMLVVMPEGLSSERVAISR
                     AYGAEVLFCGTFHVNDALERARELGRQPGCFFPGQFESEWNVDENRELLGPEILAQLP
                     PGRVPDAFVHGVGTGGTLIGVGQALRKVNPAVRLFAMEPSESRTILCGEVALHQIEGI
                     SDGFVPGIFSRHRDLVRDTISVSSDDAVAEMRRLARAHGLLCGPSSGAHMLAAQRIRA
                     RYPELRTVVTILDDEGEKYLHDFYMPPAPGTTRPLPFH"
     gene            complement(1290..2330)
                     /locus_tag="PKN91_10025"
     CDS             complement(1290..2330)
                     /locus_tag="PKN91_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS28445.1"
                     /translation="MEYPFGDELPALGRTLEVAPGVRWLRMPLPFALNHINLWLLRDR
                     DVESGREGWALIDCGIADDKTRAAWEQVFASELQGLPVLRVIVTHMHPDHIGLAHWLT
                     ERFGVRLWISATDFGAARLASQSTTGFGGDSAAAFFASHGLNDPDSVAKVRQRSNYYA
                     GMVPQVPARFRRMMDGNVLRIGAHDWTCHAGYGHAPEHISLYCPEFGILVSGDMVLPR
                     ISTNVSVLDLEPEADPLPLYLDSIARMRALPAGTLVLPSHGKPFRGLHERIGQLVAHH
                     DERFDAVLTACAREPQTAASLLPVLFHRRLDLHQTTFAMGEAIAHLNALWYRGQLRRE
                     RGADGVYRFVPA"
     gene            complement(2409..3794)
                     /locus_tag="PKN91_10030"
     CDS             complement(2409..3794)
                     /locus_tag="PKN91_10030"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNS28446.1"
                     /translation="MGAATLESMWRQLVGPAADANVVGSITWAQLLVSASFLALALVG
                     TAVVAWVVRRGASASGDGTLVRESLGHMARALGKPLHVLIWAAGLYFAATPFVALTGP
                     QGRAGSIQALSRLAFDLALFVIVIWLAIRVTRVLDERLVQWAARSDKRLDDFLLPVIG
                     RSLRIAVPVIGVILALPLLHLPARFDAASGKFASILLIVALAGILLQTVQIGARVILA
                     GHDVNAADNLRARKIHTQVGIITKVLGVVIVIFAIASVLMLFEEVRRLGTSLLASAGV
                     AGIILGMAAQKTIANLLAGFQIALTQPIREDDVLIVEGEWGRVEEITLTYVVVRIWDE
                     RRLVLPLSYFIEKPFQNWTRTSANRLGSVYVWVDYAFPVEEGRKVLTGIIEGNPLWDG
                     RFWNLVVSDATERTMQLRVLATAKDASTEWDLRCDIREKFIAFIRDRHPESLPQLRMQ
                     SSGQLDAATRA"
     gene            complement(3997..4941)
                     /locus_tag="PKN91_10035"
     CDS             complement(3997..4941)
                     /locus_tag="PKN91_10035"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNS28447.1"
                     /translation="MGLLIDGEWHDEWYDTADGEFRREEASFRDWLTADGGAGPGGQR
                     GHRAEAGRYRLYVSYACPWAHRTLIVRALKGLENLVPVSVVHWRMLEHGWTFADGPGV
                     TPDPVMGAQFLYQLYTRARPGMTGRVTVPLLWDQATNTVVNNESSELIRMLNTAYDGL
                     GAKPGDYYPEPLRGAIDEINARVYPNVNNGVYRAGFATTQSAYENAVRALFETLDWLE
                     QRLSAQNWLVGGRLTEADIRLFTTLLRFDAVYHGHFKCNLRRIADYPALHAFTARIME
                     IPGVRETVHFDHIKRHYYESHRTINPTGIVPLGPLQPY"
     gene            complement(4950..5954)
                     /locus_tag="PKN91_10040"
     CDS             complement(4950..5954)
                     /locus_tag="PKN91_10040"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HNS28448.1"
                     /translation="MSNVAKPAFGSVLTDTMAVAEFRDGRWQPHALQPVGPIPMHPAA
                     HVLHYGSECFEGFKAYRGKDGSVSVFRMDRHIERMRQSARQLVLPEPDAAQLATMVRE
                     VVRASRDQVPEKPGALYLRPMLIGTMPNIGAAATPSTTALLIVLASPVWDYFAGGAKP
                     LRIFVEDQKTRTASHLGVVKTGGNYAAALGPTLEARRQYAVDQVLFCPAGAVQETGAA
                     NFLLLREGHLLTRSLDSTFLHGVTRDSLLTMAPDLGIRVEEREFDVAEMFEWVKTGEA
                     ALSGTAAVLAGVGTLVTRSGEHRVGTGEIGPVTQRLRTALVAIQTDEAPDTRGWLERI
                     "
     gene            complement(5951..7201)
                     /locus_tag="PKN91_10045"
     CDS             complement(5951..7201)
                     /locus_tag="PKN91_10045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNS28449.1"
                     /translation="MPDLGEGTVSAEVLQWHVKTGDMVKEDQIIADVMTEKAAIEIPA
                     PVSGRVVRTQGEPGEMIAVGSELIVFDTGGDALSAGASEAAAVDAAAPPAAAAIAVSA
                     APAAAPAPACARVMASPATRRRAKEAGIDLQTIAGTGPGGRVTREDFEAVASGAAAAG
                     RAAGAAGRPATAPAARAPRTGTTEVKVIGLRRVIAQRMSEAKRNIPHFAYVEEVDITE
                     LEALRVHLNATAPEGTPSLTLLPFLGLALARVLPAYPQCNALYDAERNVVVQHAALHL
                     GIATQTPDGLKVPVVRHAESLTLRELAAAIRDVADRARRNKSPREELSGSTLTITSLG
                     RMGGIASTPVINAPEVAIIGVNKAIERPVVRGGTVAVRLVMNLSSSFDHRFVDGYDAA
                     SMIQALKSLLEHPATIFMQEEVGR"
     gene            complement(7236..>7867)
                     /locus_tag="PKN91_10050"
     CDS             complement(7236..>7867)
                     /locus_tag="PKN91_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNS28450.1"
                     /translation="SQSPEAVFAHICGIKVVMPSNPYDAKGLLIAAIEDDDPVIFLEP
                     KRLYNGPFDGDPEKPAVPWSAHPAGEVPEGHYTVPISKAQVVRRGGDVTIVTYGTMVH
                     VAVAAAQSLGVDAEVIDVRTIVPLDTQTLCESVQRTGRCLIVHEATRFAGFGAELSAT
                     IQEQCFWQLQAPIGRVGGWDTPYPHAFEWEYFPGQQRVAKALEAVMQAS"
BASE COUNT         1304 a         2707 c         2622 g         1234 t
ORIGIN      
        1 aaaaatacca gcggcggtaa ccttgaaatg ataccaccaa ggagattgca atcatgaccg
       61 acacctggct cagcacgctc atcaccgaca cgccacaggc aggcttcgaa ctcgcgatca
      121 cgctgagtcg ccgcggcgtg aagtacacgc agccggacac cgcggtgctc aagaagctgc
      181 gccccgccta tgcggattcc gcggacggac tcacggccgc gtcacaggtg atcgcgatca
      241 acttccagac ggtcgccgcc gcgaacaatt actggcgtac cgcctgactc tgcgcttcac
      301 gaccatgcgc tacgccaccg tctccgccag catcctcgat gccatcggcg ataccccgct
      361 gctcgatatc gacggcgtgt tcgccaagtg cgagttcgtg aacccgtccg gatcgatcaa
      421 ggcgcgcatc gcgcactaca tcgtcgagcg cgccgagaag gcgggcctgc tcaagcccgg
      481 cgacaccatc gtcgaagcga ccagcggcaa caccggcaat gcgttctcga tggtggccgc
      541 ggtcaagggc taccgcatgc tggtcgtgat gcccgaggga ctgtcgagcg agcgggtggc
      601 gatctcgcgc gcctacggcg ccgaggtgct cttttgcggc accttccacg tcaacgacgc
      661 cctcgagcgg gcgcgcgagc tgggccggca gcccgggtgc ttctttcccg gccagttcga
      721 gtcggaatgg aatgtggacg agaatcgcga gctgctcggg ccggaaatcc tcgcccagct
      781 gccgccaggc cgcgtacccg acgccttcgt gcacggcgtc ggcaccggag gcacgctgat
      841 cggtgtcggc caggcgctgc gcaaggtcaa tccagccgtg cgtctgttcg ccatggagcc
      901 gtccgagtca cgcaccatcc tgtgcggaga ggtggcgctg caccagatcg aaggcatttc
      961 cgacggcttc gtccccggca tcttctcgcg ccatcgcgat ctcgtgcgcg atacgatcag
     1021 cgtctcgagc gacgatgcgg tcgcggaaat gcgcaggctc gcccgcgccc acgggctcct
     1081 gtgcggaccg agctccggcg cgcacatgct cgcggcgcag cgcattcgcg cgcgctatcc
     1141 ggaattgagg acggtggtca cgatcctcga tgacgagggc gagaaatacc tgcacgactt
     1201 ctacatgcca ccggcccccg gcacgacccg gccgctgccg tttcactgat gaccgccgcg
     1261 gcgggcgcgc gagaccccgg cgatccggct caggccggaa cgaagcggta gacgccgtcc
     1321 gcgccgcgct cacgccgcag ctggccgcga taccacagcg cattcaggtg ggcgatcgcc
     1381 tcgcccatcg cgaacgtggt ctggtgcagg tcgagcctgc ggtggaacag caccggcagc
     1441 agcgaggcgg ccgtctgcgg ctcgcgcgcg caggcggtga gcaccgcatc gaagcgctcg
     1501 tcgtggtgcg cgaccagctg cccgatgcgc tcatgcagtc cgcggaaggg cttgccgtgc
     1561 gagggcagca ccagcgtgcc ggccggcagc gcgcgcatcc gcgcgatcga gtcgaggtag
     1621 agcggcaacg gatcggcctc gggctcgagg tcgagcacgc tgacattggt cgagatgcgg
     1681 ggcagcacca tgtcgccgct caccaggatg ccgaactcgg ggcaatacag cgagatgtgt
     1741 tcgggagcgt ggccatagcc ggcatggcag gtccagtcat gcgcgccgat gcgcagcacg
     1801 ttgccatcca tcatgcggcg aaaccgcgct ggcacctgcg gcaccatgcc cgcatagtag
     1861 ttgctgcgct ggcgcacttt cgcgaccgaa tcggggtcgt tgaggccgtg gctggcgaag
     1921 aaggcggccg cgctgtcgcc gccgaagccg gtcgtggact ggctcgcgag ccgcgcggcg
     1981 ccgaagtcgg tggcgctgat ccacagccgc acgccgaagc gctcggtgag ccagtgcgcg
     2041 aggccgatgt gatccgggtg catgtgggtg acgatcaccc gcagtacggg cagcccctgc
     2101 agttcgctcg cgaacacctg ctcccaggcc gcgcgggtct tgtcgtcggc gataccgcag
     2161 tcgatcagcg cccagccctc gcgtccgctc tcgacgtcgc gatcccgcag cagccagagg
     2221 ttgatgtggt tgagcgcaaa gggcagcggc atgcgcagcc agcgcacgcc gggcgcgact
     2281 tcgagcgtgc gaccgagtgc gggcagctcg tcgccgaagg ggtactccaa tcgggattcg
     2341 tcgggattca tgcaacacgc tttcgacagg catcggcttg atcgccgatg ccgccagtct
     2401 atgccagctc aagcccgtgt ggcggcgtcg agttggccgc tcgactgcat gcgtagctgc
     2461 ggcaggctct ccgggtggcg gtcgcggatg aaggcgatga acttctcgcg gatatcgcag
     2521 cgcaggtccc actcggtcga ggcatctttg gcggtggcga gcacccgcag ctgcatcgtc
     2581 ctttcggtgg catcgctcac gacgaggttc cagaagcgcc cgtcccacag cgggttgccc
     2641 tcgatgatgc ccgtgagcac tttgcgcccc tcctcgaccg gaaaggcgta atcgacccag
     2701 acgtagaccg acccgaggcg gttcgcggag gtacgcgtcc agttctggaa cggcttttcg
     2761 atgaagtagc tcagcggcag caccaggcgc cgctcgtccc agatgcgcac gacaacgtaa
     2821 gtcagcgtga tctcctccac ccgcccccat tcgccctcga cgatcagcac atcgtcctcg
     2881 cgaatgggct gggtcagggc aatctggaaa cccgcgagca ggttcgcgat ggtcttttgc
     2941 gcggccattc caaggatgat gccggccacc cccgccgaag cgagcaggct cgtacccaat
     3001 cgccgcactt cctcgaagag catcaggacc gacgcgatgg caaagattac gatgacgacg
     3061 cccagcacct tggtgatgat ccccacctgg gtatggattt tccgggcgcg cagattgtcc
     3121 gccgcattga cgtcatggcc ggcgaggatg acccgcgcac cgatctgcac ggtctgcagc
     3181 aggatgcctg ccagcgccac gatgagcagg atgctggcga atttcccgct tgccgcatcg
     3241 aagcgcgccg gcaggtgcaa cagcggcagc gcgaggatga cgccgatcac cggaaccgcg
     3301 atgcggagac ttcgcccgat gacaggcagg agaaaatcgt ccagccgctt gtcgctgcgc
     3361 gcggcccact gcacgagccg ctcgtccagc acgcgcgtga cacggatggc cagccagatc
     3421 acgatgacga agagcgccag gtcgaacgcc aatcgggaca gggcctgaat cgagccggcc
     3481 cgcccttgcg gacccgtgag cgcgacgaac ggcgtggcgg cgaaatacag cccggcggcc
     3541 cagatcagca catggagcgg cttgcccagc gcccgcgcca tgtgacccag cgactcccgc
     3601 accagcgtgc catcgccgga tgcgctggca ccgcgacgca cgacccaggc gacgacggcg
     3661 gtgcccacca gcgcaagcgc caggaagctc gcgctgacga gcaactgcgc ccacgtgatg
     3721 ctgccgacga cattggcatc cgcggcgggg ccgacgagtt gtctccacat ggattcgagc
     3781 gtggcggcgc ccacggctac tccccgatga tggaaaagga tgtgtaccag gttgaaaaat
     3841 ccatggtgcc tccctggagt ggcctgtgcg cggccacttc agcctacgga aatttccgcg
     3901 gcgattaaac cccgcgagcg aagcacggtg ctgccgcgcg ggcatgagcc ggcgagccgg
     3961 tggcgaacac gcgcgcaatc gcgcggttcc cgcgcctcag tacggttgca gcggccccag
     4021 gggcacgatc ccggtcggat tgatcgtgcg gtgactctcg tagtaatgcc gcttgatatg
     4081 atcgaagtgc accgtttcgc gcacgcccgg gatctccatg atccgcgccg tgaacgcatg
     4141 cagggcggga tagtcggcga tgcgccgcag attgcacttg aagtgtccgt ggtagaccgc
     4201 atcgaagcgc agcagcgtcg tgaacaggcg gatatcggcc tcggtcagcc ggccaccgac
     4261 cagccagttc tgcgccgaca gccgctgctc gagccagtcc agggtttcga acagcgctcg
     4321 cacggcgttc tcgtacgcgg actgcgtggt ggcgaaaccg gcgcggtaca cgccgttgtt
     4381 gacgttcgga tacacgcgcg cattgatctc gtcgatcgca ccccgcagcg gctccgggta
     4441 ataatcgccc ggcttcgcgc cgaggccgtc atacgccgta ttgagcatgc ggatgagttc
     4501 ggacgactcg ttgttgacga cggtgttcgt ggcctgatcc cacagcagcg gcaccgtcac
     4561 ccgcccggtc ataccaggcc gcgcgcgggt gtagagctga tacaggaatt gcgcgcccat
     4621 gaccggatcg ggcgtgacgc ccgggccgtc ggcgaaggtc cagccgtgct cgagcatgcg
     4681 ccagtgcact accgagaccg gcaccaggtt ttcgagcccc ttcagcgcgc gcacgatcag
     4741 ggtgcggtgc gcccacgggc aggcgtagct gacatacagc cgataacggc cggcttcggc
     4801 gcggtgtccg cgctgtccac cgggtcccgc gccgccgtcg gcggtcagcc agtcgcggaa
     4861 gctggcttcc tcacgccgga actcgccatc ggccgtgtcg taccactcgt catgccattc
     4921 gccgtcgatc agcaggccca tgggcgccct cagattcgct cgagccagcc ccgcgtatcg
     4981 ggcgcctcgt cggtctggat cgccacgagt gccgtgcgca ggcgctgtgt gaccgggccg
     5041 atctcgccgg tgcccacgcg atgctcgccg ctgcgcgtga cgagcgtgcc gacaccggcc
     5101 agcacggccg cggtcccgga cagggccgcc tcgccggtct tcacccattc gaacatttcg
     5161 gcgacatcga actcgcgctc ctcgacgcga atgccgagat cgggcgccat cgtgagcagc
     5221 gaatcgcgcg tgacgccatg caggaacgtg ctgtcgaggc tgcgcgtgag caagtggcct
     5281 tcgcgcagca acaggaaatt ggccgcgccc gtctcctgca cggcgcccgc cgggcagaac
     5341 agcacctggt cgacggcata ctgcctgcgc gcctccagcg tgggtccgag cgccgccgca
     5401 tagttgccgc cggtcttcac gacgcccagg tgcgaggccg tgcgggtctt ctggtcctcg
     5461 acgaagatgc gcagcggctt ggcgccgccg gcgaagtaat cccacaccgg gctcgcgagc
     5521 acgatcagca gcgccgtggt gctcggcgtc gcggcggcgc cgatattggg catcgtgccg
     5581 atcagcatgg gtcgcaggta gagcgcaccg ggcttctcgg gcacctggtc gcggctcgcg
     5641 cgcacgacct cgcgcaccat cgtggcgagc tgcgccgcat cgggctcggg cagcaccagc
     5701 tggcgcgcgc tctggcgcat gcgctcgatg tgcctgtcca tgcgaaagac gctgaccgaa
     5761 ccgtccttgc cgcgataggc cttgaagcct tcgaagcact cgctgccgta gtgcaggaca
     5821 tgcgcggcgg gatgcatcgg gatcggcccg acaggctgca gcgcgtgcgg ctgccagcgg
     5881 ccgtcgcgga actccgctac agccatcgtg tcggtcaata cgctgccaaa agccggcttc
     5941 gccacattgc tcatcgtcca acctcttcct gcatgaaaat cgttgccggg tgctcgagca
     6001 gggacttgag cgcctggatc atggatgcgg catcgtaccc gtccacgaag cggtgatcga
     6061 acgacgatga aagattcatc accaggcgaa ccgcgactgt cccgccgcgc acgacgggcc
     6121 gctcgatcgc cttgttgacg ccgatgatcg cgacttcggg cgcattgatc accggggtcg
     6181 aggcgatgcc acccatccgg cccagactcg tgatggtcag cgtggagccg gagagttcct
     6241 ctcgcggcga cttgttgcgg cgggcgcgat cggccacgtc acggatcgcc gcggccagtt
     6301 cgcgcagcgt gaggctctcc gcatggcgca ccaccggcac cttgagtcca tccggcgtct
     6361 gggtcgcgat gccgaggtgc agcgccgcgt gctgcacgac gacgttgcgc tccgcatcgt
     6421 acagcgcatt gcactgcggg taggcgggca ggacgcgcgc gagcgccagg ccgaggaagg
     6481 gcagcagcgt gagcgaaggc gtgccttcgg gcgccgtggc gttcagatgc acgcgcagcg
     6541 cctcgagctc ggtgatgtcg acttcctcga cgtaggcgaa atgcgggatg ttgcgtttcg
     6601 cctcgctcat gcgctgggcg atcacgcgcc gcaggccgat gaccttcact tccgtagtgc
     6661 cggtgcgcgg ggcacgtgcc gcaggcgccg tggccgggcg ccccgcggcc cccgcggccc
     6721 tgcccgccgc cgcggcaccc gacgcgaccg cttcgaagtc ttcgcgcgta acccgcccgc
     6781 ccggacccgt gccggcgatc gtctgcaggt cgatgcccgc ctccttcgcg cgccgccggg
     6841 ttgccggcga agccatgacg cgtgcgcagg caggcgcagg cgccgcggcg ggcgccgcgg
     6901 acaccgcgat ggcggcagcg gccggcgggg cagccgcatc caccgccgca gcctcggagg
     6961 cgccggccga cagggcgtcg cccccggtat cgaacacgat cagctcggag ccgaccgcga
     7021 tcatctcgcc gggctcgccc tgcgtccgca cgacgcgccc cgacacggga gcggggatct
     7081 cgatcgccgc cttctcggtc atgacgtcgg cgatgatctg gtcttccttc accatgtcgc
     7141 cggtcttgac gtgccactgc agcacctctg cgctgaccgt gccttcgccc aggtcgggca
     7201 tcctgaaaac gtactgcgac atcctgcccc cgtactcaac ttgcctgcat gacggcttcg
     7261 agcgccttcg ccacgcgctg ctggcccgga aaatactccc attcgaaggc gtgcggatac
     7321 ggcgtgtccc agccgcccac gcggccgatc ggcgcctgca gctgccagaa acattgttcc
     7381 tggatcgtgg cggagagttc cgcaccgaag ccggcgaagc gcgtcgcttc gtgcacgatc
     7441 aggcagcggc cggtgcgctg caccgattcg cacagggtct gcgtgtcgag cggcacgatc
     7501 gtgcgcacgt cgatgacttc ggcatcgacg ccgagcgact gcgcggccgc caccgcgacg
     7561 tgcaccatgg tgccataggt gacgatggtg acatcaccgc cgcggcgcac gacctgcgcc
     7621 ttgctgatgg gcaccgtgta gtggccctcc ggcacctcgc cggccggatg cgcgctccac
     7681 ggcacggcgg gcttctccgg gtcgccgtcg aacggtccgt tgtagagccg cttcggttcg
     7741 agaaagatca ccgggtcgtc gtcctcgatc gccgcgatca gcagaccctt ggcgtcatag
     7801 ggattcgagg gcatgacgac cttgatgccg cagatgtgcg cgaacaccgc ctcgggactc
     7861 tgcgaat
//
LOCUS       DAOQVY010000112         7837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_10240_length_7837_cov_6.145207, whole genome shotgun sequence.
ACCESSION   DAOQVY010000112 DAOQVY010000000
VERSION     DAOQVY010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10240_length_7837_cov_6.145207"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..927)
                     /locus_tag="PKN91_10055"
     CDS             complement(124..927)
                     /locus_tag="PKN91_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNS28451.1"
                     /translation="MNPTALRVLIVDDEALARQRLADLLAREQAVEIVGQIDNGDAAV
                     KAIRDLAPDLVFLDVQMPGRTGIDVVREIGATQMPATIFVTAYDQYALTAFDVAAIDY
                     LVKPFDDERFELALSRARRAIELREIGALSTRLRTLLDAPTGTPAAASAAAGRGYLER
                     IAVEMRGQIRVVPVEEIDAITASGPYVELHVGDRTYLVRERMQTLEERLDPQQFARVH
                     RSVIVRLDRIGALLREAGGDYAVRLKNGKQHPVSRSRVDQLQRWIGVLP"
     gene            complement(924..2057)
                     /locus_tag="PKN91_10060"
     CDS             complement(924..2057)
                     /locus_tag="PKN91_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNS28452.1"
                     /translation="MNALPPSSEHFPLQRAEYVMIAAFWAFFALLWSANALLATGGRA
                     VPLFPAAPVVLACVTAAIWAVLTPPLFMVAGRLSIDRGHRAARILVLLLVTVVVAFGV
                     DVLMAYLRYDVFFERPVGRRLPPGFVQRLGRLWFINQVIIVAAILAAGFARHNFRRYR
                     ARREETMRLQAQLADARLAMLRSQLDPHFLFNTLHAISALVERDPRGVRRMIARLSDL
                     LRSTLEGAGEQEVPLREELRFLDGYLDIMRTRFQGRLAIDMQVEPGLEDALVPHLVLQ
                     PLVENAIRHGTAKSDADGRIEIAARRDGAAIVLSVEDNGPGAPATATQIVEGIGLRNT
                     RERLAQLYGAAQHLALEPRHGGGMRAEVRLPFHTRTDLRAVAV"
     gene            2192..3430
                     /locus_tag="PKN91_10065"
     CDS             2192..3430
                     /locus_tag="PKN91_10065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNS28453.1"
                     /translation="MNRKPVIVAAVVLALLAGGLFAWSKLSREAAPPYRFASVERGNI
                     RAAVAATGALSAVTTVQVGTQVSGRVVATYADFNDAVKQGQLIARIDPTLLEQAVRDA
                     QAGLERSRAQYQQADIEYQRNLGLFEQKVLTEIEFNTTKFSRDVAMANMKSAQVALDR
                     ARQNLAYTEIYAPIDGIVVERNVDVGQTVAASLSAPQLFLIAQDLSRMQILASVAESD
                     IGAIREDQPVSFTVQTWPNETFSGVVRKVRLQSTTIENVVSYTVVIEVANPEGKLLPG
                     MTASVDFITGEASNVLTVPNAALRFRPTEAMLAAVRPTVGRDGAPVAADAAPGQRGAG
                     QGGTRPANAAVLWYLDTAGKLARARVRTGLTDGTRTEVSGEEITEGLDVIVGVTQTSA
                     STATSSPFQSPAQGRRGPGF"
     gene            3433..4170
                     /locus_tag="PKN91_10070"
     CDS             3433..4170
                     /locus_tag="PKN91_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS28454.1"
                     /translation="MPPVIHLEGVTRVFNTGDNAVHALAGVDLDVHAGEMVAIMGASG
                     SGKSTLMNILGCLDKPTAGRYILDGIEVSELGRNDLADLRSRKIGFVFQGFNLLARTT
                     ALENVELPLLYYRSGQFHGKESPAPAALRRVGLGERMHHVPSELSGGQQQRVAIARAL
                     VTNPSLILADEPTGNLDSRTSIEIMALFQELNDQGITILLVTHEHDIAEHAKRIIEMR
                     DGRIVRDVPVTQRRIAADELAAAGRAA"
     gene            4167..5384
                     /locus_tag="PKN91_10075"
     CDS             4167..5384
                     /locus_tag="PKN91_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS28455.1"
                     /translation="MKKRALFSIALRSILRNKMRTLLTMLGIVIGVAAVIVMVALGQG
                     AQATIEAQIKGLGTNMLVIMAGSTTQGGVSQGAATFNRLTIADYEKLAREGTLLAAVS
                     PVIFSRGQVIGGGTNWRASIMGVAPTYQQIRDWPAQSGSFFTEADTRSVRRVVVLGST
                     VANALFADSDPVGQQIQLRNVPFTVIGVLSAKGQTAGGSDQDDTVIIPYTTAQAHLAG
                     RSFIGQIIANTFSPGDVPAAQEEIRSIMREAHKIAPGDADDFTIRNQDEIAAAASGTT
                     RVMSMLLAAIASISLVVGGIGIMNIMLVSVTERTREIGIRMAIGARSSDVLAQFLVES
                     IVISLAGGLIGIVLGYGSSILLARLTGWAVAMPPEAALIAVAFSAAVGVFFGFYPARK
                     ASALDPIQALRYE"
     gene            5377..6708
                     /locus_tag="PKN91_10080"
     CDS             5377..6708
                     /locus_tag="PKN91_10080"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNS28456.1"
                     /translation="MNRTPLARLAAAVCATLAIGTAGAQAPAADTRIISFDQAVEIAL
                     EQNDTLRQARNATALGALDVEQARRDFLPDLRLGSRTSQGFGAQGDNGPRTTGIDLSS
                     GVTLFDGFANTATLQGAKFASSASDAELARAAETVVFTVASNFLLLVEQQEQLRVRRE
                     NLAAQAALEAQIQLYVNVGARTIADLYQQQASVASARLAVVQAERASELARIEVIRTL
                     RLDPRGSYEFVAPAITDPQQITIDPLDALIGRALENRADIRAGEARLEASRQEIRVAK
                     SARWPVVTLSAGYGSSFSSDSDADWTHQLDDLRGGSVNLGVSIPLFDRSATRIATRRA
                     ELRAENAQIFLDALEQEVGSQVRTAVLDLESARAQLLTARAQLRAAEQALEFTDQRYK
                     AGAATLVELTQSRATQVEAASAAVTALYGVEFRRRLLDYYVGGLTADSMRF"
     gene            6746..7516
                     /locus_tag="PKN91_10085"
     CDS             6746..7516
                     /locus_tag="PKN91_10085"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNS28457.1"
                     /translation="MDFWSVIWILFASGTLATAVGAAPVLVLGSLNPRIANHFLAIAA
                     GVMLAASVYGLLPAALDEAGHLVAPAWAAPFTLLAMACGALAVAWTNRHVPHVHQFKG
                     QDGGGSGRPMSVLLVTAIALHNFPEGLSVGVTIGAQDETLTPRIATAIAAQNMPEGLV
                     VAMAMLGSGVGRLRAFGLGVLTGLIETAGGLLGAAAVGAAAGLLPWALAFAAGAMLYV
                     ISEEVIPETHRRGMSGGATASLLAGFGAMVWFDALMAF"
     gene            7529..>7837
                     /locus_tag="PKN91_10090"
     CDS             7529..>7837
                     /locus_tag="PKN91_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28458.1"
                     /translation="MYAAQTKFRAPRVRADSVVRAPLLATLEASVDNYPVTLLSAPAG
                     WGKTTLLTQYASAAHAARQVVWLSIDSDDNNPNRLFAALVEAIEPLGAVWDIDPVAFT
                     A"
BASE COUNT         1270 a         2719 c         2661 g         1187 t
ORIGIN      
        1 cgcgggaccg ctacggcatc accgatgcga tggtgcggct gtcggtcggc atcgagcacc
       61 cggacgacct gatcgccgac ctcgcgcagg cgctcgccga ggactagctc cgcgcgcgac
      121 gcctcagggg agcacgccga tccagcgctg cagctggtcg acgcggctgc gactgaccgg
      181 atgctgcttg ccattcttga gccgcaccgc gtaatcgccg cccgcctcgc gcagcagcgc
      241 gcctatgcga tcaaggcgca cgatgaccga gcggtgcacg cgcgcgaact gctgcgggtc
      301 gaggcgttcc tccagcgtct gcatgcgctc gcgcaccagg taggtccggt ccccgacatg
      361 cagctcgaca tagggcccgc tcgccgtgat cgcgtcgatc tcctcgaccg gcaccacgcg
      421 tatctggccg cgcatctcga ccgcgatgcg ctcgaggtaa ccgcgcccgg cggcggcgct
      481 cgccgcggct ggcgtgcccg tcggggcgtc gagcagcgtg cgcagccgcg tcgacagcgc
      541 accgatctcc cgcagctcga tcgcgcgccg cgcgcgggac aacgccagct cgaagcgctc
      601 gtcgtcgaag ggcttcacga ggtagtcgat ggctgcgacg tcgaaggccg tgagcgcata
      661 ctggtcatag gcggtgacga agatcgtcgc cggcatctgt gtcgcaccga tttcgcgcac
      721 gacatcgata ccggtgcgac cgggcatctg cacatcgagg aagacgagat ccggggcaag
      781 atcgcgaatg gcttttaccg cggcatcacc attgtcgatc tggccgacga tctcgaccgc
      841 ctgctcgcgc gccagcaggt ccgcgagccg ctggcgcgcg agagcctcgt cgtcgacgat
      901 cagcacgcgc agggctgttg gattcatacg gccaccgcgc gcaggtcggt gcgcgtgtgg
      961 aatggcagcc gcacctcggc acgcatcccg cctccgtggc gcggctcgag cgcgaggtgc
     1021 tgggcggcgc cgtagagctg cgcgagccgc tcacgggtat tcctgagacc gataccctcg
     1081 acgatctgcg tcgcggtggc cggtgcgccc gggccgttgt cctccacgga cagcacaatc
     1141 gcggcgccgt cgcggcgcgc ggcaatttcg atgcgcccat ccgcatcgct cttggccgtg
     1201 ccgtgccgga tcgcattttc gaccagcggc tgcagcacga ggtggggcac caacgcgtcc
     1261 tcgagacccg gctcgacctg catgtcgatc gcgagccggc cctggaaccg ggtgcgcatg
     1321 atgtcgagat acccgtcaag gaagcgcagt tcctcgcgca gcggcacttc ctgttcgcct
     1381 gcgccctcga gcgtcgagcg cagcaggtcc gacagccgcg caatcatgcg gcgcacgccg
     1441 cgcggatcgc gttccaccag cgccgagatc gcgtgcagcg tgttgaagag gaagtgcgga
     1501 tcgagctggc tgcgaagcat cgccaggcgt gcatcggcaa gctgcgcctg caaccgcatg
     1561 gtctcctcgc ggcgcgcccg gtagcgacgg aagttgtggc gcgcaaagcc ggcggcgagt
     1621 atcgcggcaa cgatgatgac ctggttgatg aaccacaggc gcccgagccg ctgcacgaat
     1681 ccgggcggaa gtctgcggcc caccgggcgc tcgaagaaca cgtcgtagcg caggtacgcc
     1741 atcaggacgt cgacaccgaa ggccaccacc acggtaacga gcagcagcac aaggatgcgg
     1801 gctgcgcggt ggccgcggtc gatgctcagg cgcccggcca ccatgaacag cggcggcgtg
     1861 agcacggccc agatcgcggc ggtgacgcac gcgagcacca ccggcgccgc gggaaacagc
     1921 ggcacggcac ggccgccggt cgcaagcagc gcgttggcgg accacagcag ggcgaagaac
     1981 gcccagaacg ccgcaatcat cacgtattcc gcccgttgca gcggaaagtg ttcggacgac
     2041 ggcggcagcg cattcatggt gcacaagcat aggtagctgc ggtgcgggcg gcaaaggcgt
     2101 tcacgccatt gcaggtgccg ttcgtgacac cgaggtgcaa ttggggcatt cccagctcca
     2161 ccgggggtcg cgcggccgct attctcgccc catgaacaga aagccggtca tcgtcgccgc
     2221 cgtcgtgctc gccctgctgg cgggcggcct cttcgcctgg tcgaagctct cgcgcgaagc
     2281 cgcgcccccc tatcggtttg cgtccgtgga gcgcggcaac atccgcgccg cggtggcagc
     2341 caccggcgcg ctcagcgccg tgacaaccgt gcaggtcggt acgcaggtgt cgggccgggt
     2401 ggtggccacg tatgccgact tcaacgacgc ggtgaagcag ggccagttga tcgcgcgcat
     2461 cgacccgacg ctgctcgagc aggccgtgcg ggatgcacag gccgggctcg agcgcagccg
     2521 cgcgcaatac cagcaggccg acatcgagta ccagcgcaac ctggggctgt tcgagcagaa
     2581 ggtgctcacc gagatcgaat tcaacacgac gaaattctcg cgcgacgtgg cgatggcgaa
     2641 catgaagtcg gcgcaggttg cgctcgacag ggcacgccag aaccttgcct acaccgagat
     2701 ctacgctccg atcgacggca tcgtcgtcga acgcaacgtc gatgtgggcc agacggtggc
     2761 ggcgagcctg tccgcaccgc agctgttcct catcgcgcag gacctgtcgc gcatgcagat
     2821 cctggcctcc gtcgccgaga gcgacattgg cgcaatccgc gaggaccagc cggtcagttt
     2881 caccgtgcag acgtggccga acgaaacgtt ctccggcgtc gtcagaaagg tgcgcctgca
     2941 gtcgactacc atcgagaacg ttgtcagcta taccgtcgtc atcgaagtgg cgaacccgga
     3001 aggcaagctg ctacccggca tgaccgcgag cgtcgacttc atcaccggcg aggcgagcaa
     3061 tgtgctgact gtgcccaacg cggcgctgcg cttcaggccg accgaagcga tgctggctgc
     3121 cgtgcgcccc acagtcggcc gtgacggggc gccggtcgcg gccgatgccg cgcccggcca
     3181 acgtggcgca ggccagggcg gcacacgccc agccaatgcc gcagtgctat ggtacctcga
     3241 taccgcgggc aagctcgccc gtgcgcgcgt gcgcaccggc ctcaccgacg gcacgcgcac
     3301 cgaagtgagc ggcgaggaga tcaccgaggg cctcgacgtg atcgtcggcg tcacccagac
     3361 ctcggcgtcc accgcgacgt cgagcccctt ccagtcaccg gcgcagggcc ggcggggccc
     3421 ggggttctga ccgtgccgcc ggtcatccac ctcgagggtg tcacgcgcgt gttcaacacg
     3481 ggcgacaacg ccgtgcatgc gctcgccggg gtcgatctcg atgtccacgc gggcgagatg
     3541 gtggccatca tgggagcatc gggttccggc aagtcgaccc tgatgaacat cctcggttgc
     3601 ctggacaagc ccacggccgg gcgctacatc ctcgatggca tcgaggtgag cgaactcggt
     3661 cgcaacgatc tcgccgacct gcgcagccgc aagatcggtt tcgtgttcca gggcttcaac
     3721 ctgctggcgc gcaccaccgc gctcgagaat gtcgagttgc cgctgctgta ttaccgcagc
     3781 gggcagttcc acggcaagga gtcgccggcg cccgcggcgc tgcgtcgggt cgggctcggc
     3841 gagcgcatgc accacgtgcc gagcgagctg tccggcggcc agcagcagcg cgtcgcaatc
     3901 gcgcgcgcgc tcgtcaccaa tccgtcgctg atcctcgcgg acgagcccac cggcaacctc
     3961 gattcacgca cttcgatcga gatcatggca ctcttccagg agctgaacga ccagggcatc
     4021 acgatcctgc tcgtcaccca cgagcacgac attgccgaac acgcgaagcg catcatcgag
     4081 atgcgtgacg ggcgcatcgt gcgcgacgtg ccggtgacgc agcggcgcat cgccgccgat
     4141 gaactcgcgg ccgcaggacg cgcggcatga agaagcgcgc cctgttcagc atcgccctgc
     4201 gcagcatact gcgcaacaag atgcgcacgc tgctgaccat gctcggcatc gtgatcggtg
     4261 tcgccgccgt gatcgtcatg gtcgcgctcg gccagggcgc acaggcaacg atcgaggcac
     4321 agatcaaggg actcggcacc aatatgctgg tgatcatggc gggctcgacc acccagggcg
     4381 gcgtcagcca gggagctgcc acgttcaacc gcctgaccat tgccgattac gagaagctcg
     4441 cgcgcgaagg cacgctgctc gccgccgtgt cgccggtgat cttctcgcgc ggccaggtga
     4501 tcggcggcgg cacgaactgg cgcgcctcga tcatgggggt tgcgccgacc taccagcaga
     4561 tccgcgactg gccggcgcag agcggcagtt tcttcaccga ggccgacacg cgctcggtgc
     4621 gccgtgtcgt cgtgctgggc agcacggtcg ccaatgcgct gttcgcggat tccgacccgg
     4681 tcggccagca gatccagctg cgcaacgtgc catttaccgt catcggcgtg ctgagcgcca
     4741 aggggcagac cgcgggcggc tcggatcagg acgacacggt catcatcccc tacacgaccg
     4801 cgcaggcgca cctcgccggc cgcagcttca tcggccagat catcgccaac acgttctcgc
     4861 ccggcgatgt cccggccgcg caggaggaga tccgctcgat catgcgcgaa gcgcacaaga
     4921 tcgcccccgg tgacgccgac gacttcacga tcaggaatca ggatgagatt gccgccgccg
     4981 cgagcggcac gacccgcgtg atgtcgatgc tgctcgccgc gatcgcgtcg atatcgctgg
     5041 tcgtgggcgg catcggcatc atgaacatca tgctggtgtc ggtgaccgag cgtacgcgag
     5101 agatcggcat ccgcatggcg atcggcgcac gcagcagcga cgtgctggcg cagttcctcg
     5161 tcgagagcat cgtcatcagc ctcgccggcg gattgatcgg catcgtgctc ggctacggca
     5221 gcagcatcct gctcgcgcgg ctcaccggct gggcggtggc gatgccgccc gaggcggcat
     5281 tgatcgccgt ggcgttctcg gcagccgtcg gcgtgttctt cggcttctat ccggcgcgca
     5341 aagcgtcggc tctcgacccg atccaggcac tgcgatatga ataggactcc acttgcgcgg
     5401 cttgctgccg cggtctgcgc cacgctggcc atcggcacgg caggcgcaca ggcgccggcg
     5461 gccgacactc gcatcatcag tttcgaccag gccgtcgaga tcgcgctcga gcagaacgac
     5521 acgctgcgcc aggcgcgcaa tgcgacggcg ctcggcgcac tcgacgtcga gcaggcgcgc
     5581 agggatttcc tgccggacct gcgcctcggc agccgcacat cgcagggctt cggcgcgcag
     5641 ggcgacaacg gcccgcgcac gaccggcatc gacctgtcct cgggcgtcac gctgttcgat
     5701 ggcttcgcga acaccgctac gctgcagggt gcgaagttcg cgagcagcgc gagcgatgcg
     5761 gagctcgcgc gcgccgccga gacggtggtg ttcacggtcg cgagcaactt cctgctgctg
     5821 gtcgagcagc aggagcaact gcgcgtgcga cgcgagaacc tcgccgcgca ggcggcgctc
     5881 gaggcacaga ttcaactcta cgtgaacgtc ggcgcgcgca cgatcgccga cctgtaccag
     5941 cagcaggcca gcgtcgccag cgcgcggctc gcggtcgtgc aggccgagcg cgcctcggaa
     6001 ctcgcccgga tcgaagtgat ccgcacgctg cgcctcgatc cgcgtggcag ctacgaattc
     6061 gttgcgccgg caatcaccga tccacagcag atcacgatcg atccgctcga tgcgctgatc
     6121 ggccgcgccc tcgagaaccg cgcggatatc cgcgccggcg aggctcgcct cgaggcatcg
     6181 cgccaggaga ttcgcgtcgc gaaatccgcg cgctggcctg tcgtcacgct gtccgctggt
     6241 tatgggtcct cgttctcgag cgattccgat gccgactgga cccatcagct ggacgatctg
     6301 cgcggcggct cggtcaacct cggcgtgtcg attccgctct tcgatcgcag cgccacgcgc
     6361 atcgccaccc gccgcgcgga gctgcgcgcc gagaatgcgc agatcttcct cgacgcgctc
     6421 gagcaggaag tcggctcgca ggtacgcacg gcggtgctcg acctcgagtc ggcgcgggcg
     6481 caactgctca cggcgcgtgc gcagctgcgt gccgcggagc aggcgctgga gttcaccgac
     6541 cagcgctaca aggcgggcgc cgcgactctg gtcgagctga cccagtcgcg cgccacccag
     6601 gtggaggcgg cgagcgcggc cgtcactgcg ctttacggcg tcgagttccg ccgccgcctg
     6661 ctcgactact atgtcggggg actgaccgcc gacagcatgc gcttctaggc cgcataatgg
     6721 gacgctggac cgttgggagg tcggcgtgga cttctggtca gtcatctgga tcctgttcgc
     6781 atcgggcacg cttgccaccg cggtcggcgc ggcgcccgtg ctcgtgctcg ggagcctgaa
     6841 cccgcgcatc gccaatcact tcctcgccat cgcggccggc gtgatgctcg ccgcgagcgt
     6901 ctacggcctg ctgcccgccg ccctcgacga agccgggcac ctcgtggcac cggcctgggc
     6961 ggcgccattc acgttgctcg cgatggcgtg cggagccctg gccgtggcct ggaccaatcg
     7021 tcacgtgccg cacgtgcatc agttcaaggg ccaggacggc ggcggcagcg ggcgcccgat
     7081 gtcggtactg ctcgtaaccg cgatcgccct gcacaacttc cccgagggcc tcagcgtcgg
     7141 cgtgaccatc ggcgcgcagg acgagacgct cactccgcgc attgccacgg cgatcgccgc
     7201 acagaacatg cccgagggcc tggtcgtcgc catggcgatg ttgggcagcg gcgtgggtcg
     7261 cctgcgcgca ttcgggctcg gggtgctgac ggggctgatc gagacggctg gcggcctgct
     7321 cggcgcggcg gcggtcggtg ccgccgcggg cctgctgccc tgggcgctcg cgttcgccgc
     7381 cggtgcgatg ctttatgtca tcagcgagga agtcataccg gagacccacc gtcgcggcat
     7441 gtcgggcggc gccacggcca gcctgctggc gggcttcggc gcgatggtgt ggttcgacgc
     7501 gctcatggcg ttctagaatc ggcggcgcat gtacgccgcc cagacgaagt tccgtgcacc
     7561 ccgagttcgg gccgattcgg tggtgcgcgc gcccctgctg gccacactcg aggcgtcggt
     7621 cgacaactat ccggtgactc tcctcagcgc gccggcgggc tggggcaaga ccacactgct
     7681 cacccagtac gcgagcgccg cgcacgcggc tcgccaggtc gtgtggctgt cgatcgacag
     7741 cgacgacaac aatccgaacc gcctgttcgc ggcgctcgtc gaagcgatcg aaccgctcgg
     7801 tgcggtctgg gacatcgatc ccgttgcgtt cactgcc
//
LOCUS       DAOQVY010000113         7716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_10433_length_7716_cov_4.860070, whole genome shotgun sequence.
ACCESSION   DAOQVY010000113 DAOQVY010000000
VERSION     DAOQVY010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10433_length_7716_cov_4.860070"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="PKN91_10095"
     CDS             <1..879
                     /locus_tag="PKN91_10095"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNS28459.1"
                     /translation="VSKQLKLLEDELGFQIFMREGRALTRITPTGQQVIERARRVLAE
                     IKAIKRLSEDLRNEAGGSLSIGTTHTQARYVLPEVIGDFRKRYPDVELHLHQGTSEQI
                     AEMMARDQIDFAIATGSQEMFPDTTILPIYRWHRTVAVPKGHPLTRDKRLTLKGLAKH
                     PIITYSFSFTGPSSLNEIFAAAGLAPQIALTARDADVIKTYVRLGLGVGIVASVAIEP
                     GADSDLVAIDASHIFPTHTTWVGFRRDTLLRKYMYEFLHALGGHLDRRSVDRARELRT
                     AAEVAQYFAAAKLPLR"
     gene            958..1947
                     /locus_tag="PKN91_10100"
     CDS             958..1947
                     /locus_tag="PKN91_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28460.1"
                     /translation="MDRTACIGIRVASIALACLACATGCGTLAPVSSGGRAGETLGAH
                     AWTLPECPSLAAAPKAGVLTGALIATGVDLLLEQIAGSLAAAAVADRDGLAYSGVDTR
                     YLYFAHAAQAPGKAEPLAAHAGCVVVAVADRAGARPGSWCAAHETAGAGSDAFAAACG
                     TPGRALLAAVGDDTPPGGGDTRAVPVLPHLYAEIRLQPSRDGAAIRPEVVNLHYPKAL
                     QARASASRDLAFTLQLRLPEQARGANLFVLLRDLEPGKPRYEAGDLASDDSLWTVAAA
                     YQGRTPTTADIGAGLGAVNILTEVRETGDTNRFLQALAASFAATRSEYEKALQ"
     gene            1973..3832
                     /locus_tag="PKN91_10105"
     CDS             1973..3832
                     /locus_tag="PKN91_10105"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNS28461.1"
                     /translation="MRHRCLPGLALALAAFALAPTADARTAKPPLYGQDWVAITGKPL
                     AATAGAKIFERGGNAVDAACAMIAATSTMWDVLSWGGETQALVFDPRTGKVTGVNALG
                     VAPSGATAQFFRDQGMKYPPDFGPLAAVTPGTPGGIMVMLAQYGTMSLAEVLEPAIRL
                     ADGYPIDAETANRIESAKAQLKQWPDSKRVMLPHLGEAREAARAGEIFRQPELAATLR
                     KLVAAERAALDAGKDRSAAIHAAYERFYRGDIAADLVEATRALGGLFTREDLANWEVK
                     LEEPVHVNYKGIEVYKLTSWVQGPAMLQALNLLENIDLAGMGYNSARYIHTLYQAMNL
                     AFADRDFYYGDPYFPPAEPVQGLLSKAYARERAKLIRPDRNDPDIRPGDPYPFQGGRN
                     PYTRSLEQWHTVPAERVSAPPTASRLEQLERQFMSGTTTVVAADKAGWVVTVTPSGGW
                     VPAVIAGRTGIGLSQRMQSFVLDPAENPFNVVEPGKRPRATLTPSLALKDGKPYLAFA
                     VQGGDSQDQNLLQFFLNMVEFGMTVQEATEAANINSFQMRASFGGHESQPGRLLVSTQ
                     TPGWVRDELKRMGYSLQFSERTSGPINAVWFDREHGTLWGGSSHHGEDYGIAW"
     gene            complement(3814..4398)
                     /locus_tag="PKN91_10110"
     CDS             complement(3814..4398)
                     /locus_tag="PKN91_10110"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HNS28462.1"
                     /translation="MFQSFLSIRSAIVLSLLVGLAACQAQGPRVTSEAAAGVDLAGFS
                     TFDFHDPLSTDSAGYHTLTTQHLKESTERELLAHGIRRVTGTPDLLVNFAVTTRETIS
                     QGTSPRIGVSYGGWSGGGMGMGVGVSTGGGVETATEGTLTIDLVSRETNRIVWTGSIA
                     GRLPRDAASRSRSLIDTAVKAILDRYPAPHQAMP"
     gene            complement(4435..6012)
                     /locus_tag="PKN91_10115"
     CDS             complement(4435..6012)
                     /locus_tag="PKN91_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNS28463.1"
                     /translation="MSNGVLLAHSLAAGLLAITSVALALRLRSLARRVNSVDHRHRTF
                     VAEAEQNGAMRRKIEAQLLDNQQRLDHLAHHDQLTGLPNRLYLAAHLPEALAAAKRTG
                     APLAVLFLDLDRFKHINDTRGHEVGDKLLQAVAARIREAVRTDDVVVRMGGDEFIVVL
                     GTVKNSEQVNETAARINSALATPVVIDGRPLVTTASIGVSLYPRDGQDVDALLRHSDT
                     AMYQAKDRGRNNFQVFSPVMDRKLKERVAIESSLRAAMNAGQLDLHYQPLIDVNTRRV
                     VALEALLRWQHPREGCIQPTRFIPVAEECGLIVPIGQFVLERAIADLAAWRAAGLTPV
                     PVALNVSAGQLRRGNLRSAIVSALDAHNLSPTLLQIELTEGAMFEQLEGRQGESNEDV
                     INDLRALGIRVAIDDFGTGYSSLAYLKRWRVDYLKVDRSFVRDLVTDPSDLAIVGAIL
                     AMARHMHIDVIAEGVEGFPQLEMLRKLGCRYAQGYLFAEPAPAAACERFLRARPVSFA
                     DIDLGEDVLVRAANQVA"
     gene            6221..6865
                     /locus_tag="PKN91_10120"
     CDS             6221..6865
                     /locus_tag="PKN91_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28464.1"
                     /translation="MDESGSRIQQLARHGTPAEQHIAQRVLDDPRAWSLWESEHAGLM
                     RKVASAARTTGQTAALKSGCFSLIERKAPFGYLTRRRLRGEPRRRMLAIFHGDRSYTD
                     AMLSEHGNFVRAACSHMCSKHVGTVVMLDSAFQEPLARYEQLFDEYFSTFCDVTLGAH
                     DEAESLRALLPYLKHQVVGLRKAILAMPRTLPELMREAQIRRPTGDTLRLLRPR"
     gene            6908..>7716
                     /locus_tag="PKN91_10125"
     CDS             6908..>7716
                     /locus_tag="PKN91_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNS28465.1"
                     /translation="MSQPRELGFWMCTALVVGNTIGMGIFMIPAALAPYGLNALTGWA
                     ITLVGCMFLARVFARLARIYPASDGPYGYIRSTLGHWPAFLSIWSYWVATWATNATLA
                     ISVVGYLKAIAAPLAGIPSALLALALLWMLVGVNLMGARTGGRVQVVTTVIKLLPMIA
                     VMGFGAWMLFAEPGAYTRAVPVTPLTLPQTVAASTIALFAMLGLESASVPAARVKDPG
                     RNIPRATLIGTLVTAMVYIIVSTMALLLIPQSELSQSGAPFVDLLDRFLGAG"
BASE COUNT         1237 a         2704 c         2569 g         1206 t
ORIGIN      
        1 gtcagcaagc agctgaagct gctggaggac gaactcggct tccagatctt catgcgcgag
       61 ggtcgcgcgc tgacgcgcat cacgccgacc ggacagcagg tcatcgagcg tgcccggcgc
      121 gtgctcgcag agatcaaggc gatcaagcgc ctctcggagg atctgcgcaa cgaagccggc
      181 ggctcgctgt cgatcggcac gacccacacg caggcccgct acgtgctgcc ggaagtgatc
      241 ggcgacttcc gcaagcgcta tccggacgtc gagctgcatc tgcaccaggg cacgtccgag
      301 cagatcgccg aaatgatggc gcgcgaccag atcgacttcg cgatcgcgac cggctcgcag
      361 gagatgttcc ccgacaccac gatactgccg atctaccgct ggcaccggac cgtcgccgta
      421 ccgaagggcc atccgctcac gcgcgataag cgcctcacgc tgaaggggct ggcgaagcac
      481 ccgatcatca cctattcgtt cagcttcacg ggcccctcct cgctcaacga gatcttcgcc
      541 gcggcgggtc tcgcgccgca gatcgcgctc accgcgcgcg acgccgacgt catcaagacc
      601 tatgtgcgcc tcggtctcgg cgtcggcatc gtcgcctcgg tggccatcga gcccggggcc
      661 gacagtgacc tcgtggccat cgacgcctcg cacatcttcc cgacgcacac cacctgggtc
      721 gggttccgcc gcgacaccct gttgcgcaag tacatgtacg agtttctgca tgcgctcggc
      781 gggcacctcg atcggcgcag cgtcgaccgt gcccgcgagt tgcgtaccgc cgcggaggtc
      841 gcccagtact ttgccgcggc gaaactgccg ctgcgctgag ccttcgccgg cctggcgcga
      901 agcccgggtc gcgctatagt ggccgctcga cccgccggag cgggctacgg gggtgccatg
      961 gatcggaccg cctgcatcgg gattcgtgtc gcatcgattg cgctcgcctg cctcgcgtgc
     1021 gccacaggct gcggcacgct ggcgccggtg tcgtccggcg ggcgtgccgg tgaaacactc
     1081 ggcgcacacg cctggacatt gcccgaatgc ccatcgctgg ccgcggcccc gaaggcgggc
     1141 gtgctgaccg gcgcgctgat cgccacgggc gtcgacctgt tactcgagca gatcgcgggt
     1201 tcgctcgccg ccgccgccgt cgccgatcgc gacgggctgg cctactcagg cgtcgatacg
     1261 cgctatctct acttcgccca cgcggcacaa gcgcccggca aagcggagcc gcttgcggcg
     1321 catgcaggct gtgtcgttgt cgccgtagcg gatcgggcgg gcgcgcgacc gggcagctgg
     1381 tgcgcggcac acgagacggc gggcgcgggg agcgacgcat tcgccgcagc ctgcgggacg
     1441 ccagggcgcg ccctgctcgc cgcggtcggt gacgacacgc cgcccggtgg cggcgacact
     1501 cgcgccgtcc ccgttctgcc gcacctctat gcggagatcc gccttcagcc ctcgcgcgat
     1561 ggcgccgcga tccggcccga ggtcgtgaat ctgcactacc cgaaggcgct gcaggcgcgc
     1621 gcctcggcct cacgcgatct cgcgttcacg ctgcaactgc ggctgcccga gcaggcgcga
     1681 ggcgcaaacc tcttcgtgtt gctgcgcgac ctcgagcccg gcaagccgcg ctatgaagcc
     1741 ggcgacctgg cgagcgacga cagcctgtgg accgtcgccg cggcctatca gggccgcaca
     1801 ccgacgacgg cggacatcgg cgccggcctc ggggccgtca acatcctgac cgaagtgcgc
     1861 gagacgggag acacgaaccg gttcctgcag gcactggcgg cgagcttcgc cgcgacccga
     1921 tcggaatacg agaaagcgtt acagtagcgg cccccgcttc cggaggaatg tcatgcgcca
     1981 tcgctgcctg cccggcctcg cccttgcgct cgctgcattc gcactcgcgc caacggcgga
     2041 tgcgcgcacc gcgaagccac cgctgtacgg ccaggactgg gtcgcgatca ccggcaagcc
     2101 gctcgcggcc acggccggcg ccaagatttt cgagcgcggc ggcaacgccg tcgacgcggc
     2161 ctgcgcgatg atcgccgcga cctcgacaat gtgggacgtg ctctcctggg gcggtgaaac
     2221 gcaggcgctc gtcttcgacc cgcggaccgg caaggtgacc ggtgtcaacg cgctcggcgt
     2281 ggcaccgagc ggcgcaaccg cgcagttctt cagggaccag ggcatgaagt atccgccgga
     2341 cttcgggccg ctcgctgcgg taacgcccgg cacgcccggt ggcatcatgg tgatgctcgc
     2401 gcagtacggc accatgagcc tcgctgaagt gctcgagccc gcgatccggc tcgccgacgg
     2461 ttatccgatc gacgccgaga ccgccaaccg tatcgagagt gccaaagcgc agctcaagca
     2521 gtggccggat tcgaagcgcg tgatgctgcc gcacctcggc gaagcacgcg aggcggcccg
     2581 cgcgggtgag atttttcgcc agcccgaact cgccgcaacg ctgcgcaagc tcgtcgcagc
     2641 cgagcgtgcg gcactcgatg ccggcaagga ccggtcggcc gcgatccacg cggcttacga
     2701 acgcttctat cgcggcgaca tcgctgccga cctcgtcgag gccacgcgcg cgctcggcgg
     2761 cctgtttacg cgcgaggacc tcgccaactg ggaggtgaag ctcgaagagc cggtgcacgt
     2821 gaactacaaa gggatcgaag tctacaagct gaccagctgg gttcagggcc cggcgatgct
     2881 gcaggccctc aatctcctcg agaacattga ccttgcgggc atgggctaca acagtgcccg
     2941 ctacatccat acgctctacc aggcgatgaa cctcgcattc gccgaccgcg acttctacta
     3001 cggcgacccg tacttcccgc ccgccgagcc ggtgcagggc ctgctgtcga aagcctatgc
     3061 gcgcgagcgc gcgaaactca tccgcccgga tcgcaatgat ccggacatcc gcccgggtga
     3121 tccctatcca ttccagggcg ggcgcaatcc ctacacgcgc agcctcgagc agtggcacac
     3181 ggtgcccgcg gagcgcgtgt cggcaccgcc cacggcgtca cgcctcgagc agctcgagcg
     3241 ccagttcatg agtggcacga ccacggtggt cgcggccgac aaggccggct gggtcgtgac
     3301 cgtgacaccc agcggcggct gggtgccggc ggtgatcgcc ggccgcaccg gcatcgggct
     3361 gtcgcagcgc atgcagagct tcgtgctcga ccctgcggag aatcccttca atgtcgtcga
     3421 gccgggcaag cgcccgcgcg ccacgctgac accgagcctc gcgctgaagg acggcaagcc
     3481 ttatctggca tttgcggtgc agggcggcga ttcgcaggac cagaacctgc tgcagttctt
     3541 cctcaacatg gtcgagttcg gcatgacggt acaggaagcg accgaggccg cgaacatcaa
     3601 cagcttccag atgcgcgcct cgttcggcgg ccacgaatca cagccgggca ggctgctggt
     3661 cagcacccag acgccgggct gggtgcgcga cgagttgaag cgcatgggtt actcgctgca
     3721 gttcagcgag cgcacgtcgg gaccgatcaa tgccgtgtgg ttcgatcgcg agcacggcac
     3781 cctgtggggc ggctcgagcc accacggcga ggactacggc atcgcctggt gaggtgccgg
     3841 gtagcggtcc aggatcgcct tcacggccgt gtcgatgagc gacctggagc ggctcgccgc
     3901 atcgcgcggc agccgcccgg cgatactgcc ggtccacacg atgcgattcg tttcgcggct
     3961 gacgagatcg atggtcaggg taccctcggt ggcggtctcc acacccccgc cggtgctgac
     4021 accgacgccc atgcccatgc cgccgcccga ccatccgcca tacgaaacgc cgatccgggg
     4081 cgacgtgccc tgggagatcg tctcgcgggt cgtcaccgca aagttgacca gcaggtcggg
     4141 agtgccggtc acgcggcgga tgccgtgtgc cagcagttcg cgctccgtcg attccttcag
     4201 gtgctgcgtc gtgagcgtgt ggtaaccggc gctgtcggtg ctcagcggat cgtggaaatc
     4261 gaaggtcgaa aagcccgcca ggtccacgcc cgctgcggcc tcgctcgtga cccttgggcc
     4321 ttgcgcctgg caggcggcca ggcccacgag caatgaaagc acgatcgcac tgcgaatcga
     4381 caggaacgac tggaacatgg ctgcactccg gccgtgcgga agtcgcagag cgtatcaggc
     4441 cacctggttc gccgcacgca ccagcacgtc ttcgccgagg tcgatgtccg caaagctgac
     4501 cgggcgcgcg cgcaggaagc gctcgcaggc ggcggcgggc gccggctcgg caaacaggta
     4561 gccctgggca tagcggcaac cgagcttgcg cagcatctcg agctgcggga agccctcgac
     4621 cccctcggcg atcacgtcga tgtgcatgtg gcgcgccatc gcgaggatcg cgccgacgat
     4681 cgcgaggtcg ctcgggtcgg tgaccaggtc gcgcacgaaa ctgcgatcga ccttgaggta
     4741 atcgacgcgc cagcgcttca ggtacgcgag gctcgagtaa ccggtgccga agtcgtcgat
     4801 ggccacgcga atgccgaggg cgcgcaggtc gttgatcacg tcctcgttcg attccccctg
     4861 gcggccttcg agctgttcga acatcgcccc ctcggtcagt tcgatctgca gcagggtcgg
     4921 tgacagattg tgcgcgtcga gcgcgctcac gatggcgctg cgcagattgc cgcgccgcag
     4981 ttgtcctgcc gaaacattga gcgccaccgg caccggtgtc aggcccgccg cacgccaggc
     5041 cgcgaggtcg gcgatcgcgc gctcgagcac gaactggccg atcggcacga tcaggccgca
     5101 ctcctcggcg accggtatga aacgggtcgg ctggatgcag ccttcgcgcg gatgctgcca
     5161 gcgcagcaac gcctcgagcg ccacgacccg gcgcgtgttc acatcgatca acggctggta
     5221 gtgcagatcg agctgtcccg cgttcatcgc cgcgcgcagg ctcgactcga tggcgacgcg
     5281 ctccttcagc ttgcggtcca tcaccgggct gaatacctgg aagttgttgc ggccgcgatc
     5341 cttcgcctgg tacatcgcgg tatccgagtg acgcagcagc gcgtcgacgt cctggccgtc
     5401 gcgcgggtag aggctcacgc cgatgctcgc cgtggtcacg agcggccgcc cgtcgatcac
     5461 caccggcgtc gcgagtgcgc tgttgatgcg cgccgccgtt tcgttgacct gctcactgtt
     5521 cttcacggtg ccgagcacga cgatgaactc gtcgccgccc atgcgcacca cgacgtcgtc
     5581 ggtacgcacc gcctcgcgaa tgcgcgccgc aacggcctgc agcagcttgt cacccacctc
     5641 gtggccgcgc gtgtcgttga tgtgcttgaa gcgatcgagg tcgaggaaca gcaccgccag
     5701 cggcgcgccg gtgcgcttcg ccgcagcgag ggcctcgggc aggtgcgcgg cgaggtacag
     5761 ccggttcggc agcccggtca gctggtcatg gtgcgcaagg tgatcgaggc gctgctggtt
     5821 gtcgagcaac tgcgcctcga tcttgcgacg catcgcgccg ttctgctcgg cctcggcgac
     5881 gaaggtccgg tggcggtggt cgaccgagtt cacgcggcgc gccaggctcc tcaggcgcag
     5941 ggcgagcgcg accgaggtga tcgcaagcag gccggcagcg aggctgtggg cgagcaggac
     6001 tccgttggac ataaatcgtc ggcagcgtgg gcagttgcca gactatcggc cacttgcccc
     6061 gaatgctgcc gtgcatcgtg cacagaatcg gacatcagcc gccccacccc ggccgctgca
     6121 cgtaccgctg gcaccgggcg cggcgagggc gcatattcga cggtccaggg gcacggacaa
     6181 gaacggtgtc atggaccaag ccgtcacatt cgaccgactg atggacgaat caggctcgcg
     6241 gatccagcag ttggcgcgcc atggcacgcc ggccgagcag cacatcgcgc agcgcgtgct
     6301 cgacgatcca cgtgcctgga gcctctggga gagcgagcac gccggcctca tgcgcaaggt
     6361 cgcctcggcg gcgcgcacta ccggccagac cgcggcgctg aaatcgggct gcttttcgct
     6421 gatcgagcgc aaggcgccgt tcggatatct cacccgccgc agactgcgcg gcgaaccgcg
     6481 ccgacgcatg ctcgcgatct tccacggtga ccgctcctac accgatgcga tgctctccga
     6541 gcacggcaat ttcgtgcgcg cagcgtgcag ccacatgtgc tcgaagcacg tcggcacggt
     6601 ggtcatgctc gatagtgcct tccaggagcc gctcgcccgc tacgagcaac tattcgacga
     6661 atacttctcc accttctgcg acgtcacgct cggcgcgcac gatgaagccg aatcactgcg
     6721 cgcgctgctg ccctacctga agcaccaggt cgtcggactg cgcaaggcga tcctcgccat
     6781 gccgcgcacc cttccggagc tgatgcgcga ggcacagatc cggcgcccga ccggcgacac
     6841 cctgcggctg cttcgcccgc gctagcgggc acgcacccgc cccgtcacga tgccgaaccc
     6901 cggagcattg tcgcaaccac gcgaactcgg cttctggatg tgcacggcgc tggtcgtcgg
     6961 caacacgatc ggcatgggca tcttcatgat tcccgcggcg ctcgcgccgt atggtctgaa
     7021 tgcgctgacc ggctgggcga tcacgctggt cggctgcatg ttcctcgcgc gcgtgttcgc
     7081 gcgcctggcg cggatctatc ccgcctccga cggaccctat ggctacatac gctcgacgct
     7141 gggccactgg cccgcctttc tgtccatctg gagctactgg gtggcgacct gggccaccaa
     7201 cgccacgctc gcgatcagcg tcgtcggcta cctcaaggcg atcgcggcgc cgctcgccgg
     7261 cattccctcg gcgctgctcg cgctcgcgct gctgtggatg ctggtgggcg tgaacctgat
     7321 gggcgcgcgc accggcgggc gagtgcaggt cgtcacgacc gtcatcaagc tgttgccgat
     7381 gatcgccgtc atgggattcg gcgcatggat gctgttcgca gaaccggggg cgtacacgcg
     7441 ggccgtgccg gtcacaccgc tcaccctgcc acagacggtg gcggcctcga cgatcgcgct
     7501 gttcgcgatg ctcggcctcg agtcggcgag cgtgccggcc gcgcgcgtca aggatccggg
     7561 ccgcaacatc ccgcgcgcca cgctgatcgg cacgctggtc acggcaatgg tctacatcat
     7621 cgtctcgacg atggcgctgt tgctgatccc gcagtccgag ctgtcgcaat cgggcgcacc
     7681 cttcgtcgac ctgctggacc gcttcctcgg cgcggg
//
LOCUS       DAOQVY010000114         7685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_10509_length_7685_cov_5.958978, whole genome shotgun sequence.
ACCESSION   DAOQVY010000114 DAOQVY010000000
VERSION     DAOQVY010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7685
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10509_length_7685_cov_5.958978"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PKN91_10130"
     CDS             complement(<1..464)
                     /locus_tag="PKN91_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNS28466.1"
                     /translation="MTARFLAAAAHAAPILLDKAATLDKTCALIQEAGRGNVRLLGFP
                     ETFVPGYAYWLGLYPPLAQREVMLEYAAQSVDVAAGELDAVQAACAQHRVNVVLGLSE
                     RDGGTLYNSQAFIDEQGSLLAVRRKLQPTMSERSLWGQGDGSTLRVYDMPVG"
     gene            complement(461..877)
                     /locus_tag="PKN91_10135"
     CDS             complement(461..877)
                     /locus_tag="PKN91_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS28467.1"
                     /translation="MKRLNVPVEEFTTPNPITASGDLPVDELRELMQEHGVRHLPIVR
                     DGRIVGLVSDRDVRVALGLGAGHRIQVSAADLMATDLLTVSAAQPLDEVAFAMSDRKL
                     GSAIVNDERGEFLGIFTVTDALNALIEIVRGDAGRE"
     gene            complement(941..1672)
                     /locus_tag="PKN91_10140"
     CDS             complement(941..1672)
                     /locus_tag="PKN91_10140"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNS28468.1"
                     /translation="MSRGYASHRPSYPAALFDWLAAAAPARELAWDCATGSGQAAVDL
                     ARHFKRVIATDASAAQIAQAAPHTRIEYRVAPAEAPGLAARCVDLVVVAQALHWFDVD
                     AFHAAARRVLVPRGVIAEWCYGLTSVDAGPIDALMLELYHGALGPYWPPERAHIERGY
                     RDLPFPFTPIEAPRFAMQVHWTLPQFAGYLRTWSATARYAAARGRDPVAKLERQLAPH
                     WGSGARLVSWPLVMRVGRADTQTIG"
     gene            complement(1702..2064)
                     /locus_tag="PKN91_10145"
     CDS             complement(1702..2064)
                     /locus_tag="PKN91_10145"
                     /inference="COORDINATES: protein motif:HMM:NF018893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HNS28469.1"
                     /translation="MGRYVDGFVTPVPRRKLAAYRAMARKAAKVWRDHGALEYHECIA
                     DDVKPGKVTSFPQSVKLKPGEVVMFSWVVYKSRRQRDIVNDKVMRDPRLASMMDPKKL
                     PFDGSRMFWGGFKVFVSA"
     gene            complement(2099..3010)
                     /gene="aguB"
                     /locus_tag="PKN91_10150"
     CDS             complement(2099..3010)
                     /gene="aguB"
                     /locus_tag="PKN91_10150"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HNS28470.1"
                     /translation="MSSPPPRTVTVAATQFACSWDLAANLAVAERLTREAAERGAQVI
                     LLQELFETPYFCIEQDSRHLQLATTLEDNAAVRRFAPIARELGVVMPISFFERAGQAF
                     FNSIAIIDADGSVLGVYRKAHIPNGPGYQEKNYFSPGDSGFKVWNTRHARLGVGICWD
                     QWFPETARCMALMGAELLLYPTAIGSEPPPALPLNSREHWQRTQQGHAAANIMPLIAS
                     NRYGFERSRQDPEGLWIRFYGSSFIADPTGAKLVEAGEEGDAVLTASFDLTEIEALRN
                     NWFVFRDRRPDLYGAIMTLDGRPTPAP"
     gene            complement(3062..3754)
                     /locus_tag="PKN91_10155"
     CDS             complement(3062..3754)
                     /locus_tag="PKN91_10155"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HNS28471.1"
                     /translation="MSTQLPEIAPPRGTLDIDLVGDFACPWSYLGTRRLARALDHLHG
                     VTVRELRWHPFRLLRPEAGAPTNWRELLASRLPKGVSVEFAESSLAEAGESLGIRFNF
                     GKLAGVPDTTAAHRLVKLAESEGLHGTVLDAIFRAYFEQGRDIGDIEVLAAIGAECGL
                     SDAVLTALRDPQAGYAEVIDEERRLLLLGVTAIPNLLFNRQVLVPGPADEQIYVKALD
                     QALFPGADATMH"
     gene            complement(3790..4740)
                     /locus_tag="PKN91_10160"
     CDS             complement(3790..4740)
                     /locus_tag="PKN91_10160"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNS28472.1"
                     /translation="MQDMRTAVIGVGYLGRFHAQKYAQVPGSRLVAVVDADEEARHRV
                     AVETGAKPLASHHELLGEVDAVSIVTPTASHGAIALDFLRAGAHVLVEKPVTETVEQA
                     RALIEAAQAAGRVLQVGHLERFNPAVLAAEPYLSRPRFIECHRLAPYRARGTDVNVVL
                     DLMIHDIDIVQSIVGSPIAALEAVGAPVFSDEIDIANARIRFENGCIANATASRVSLK
                     TERKLRLFEDDAYLSIDLQQRILTVIRKREGTPAEGQLPVAIEEQSFEQGDALRAEIE
                     SFLDCARHGKPPVVSGEDGLRALETAIRITREVSASARRR"
     gene            4761..5051
                     /locus_tag="PKN91_10165"
     CDS             4761..5051
                     /locus_tag="PKN91_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28473.1"
                     /translation="MTSPIAAYRARSPLAREALLALIALIVGVTLLPLAVYYTGVEIL
                     GPYSEGGLWRFWGDFFKGLARGGLSWWLIALGPYALLLFARGARGALRWTAR"
     gene            5147..5359
                     /locus_tag="PKN91_10170"
     CDS             5147..5359
                     /locus_tag="PKN91_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28474.1"
                     /translation="MKNISERAPETPAEAVAARDTGRSENTLKRVSLALLHEIASDDR
                     ERGNDPYNSNAGNAQAAIWGRQGRRR"
     gene            complement(5465..5737)
                     /locus_tag="PKN91_10175"
     CDS             complement(5465..5737)
                     /locus_tag="PKN91_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28475.1"
                     /translation="MLIGIVMGSTLSISIALLLTGIVFLLLPEYADRLAAERVPLLRA
                     LALALLLTVLAAASFIGEIRARGWRLAAHSALIAGLLLAGWVYWPR"
     gene            5856..>7685
                     /locus_tag="PKN91_10180"
     CDS             5856..>7685
                     /locus_tag="PKN91_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNS28476.1"
                     /translation="MQTTHRLSAGFTASAIIAVMLALVSSSQAPASTSALPPGAIAPG
                     ERHRLVARRVGMILEDTHYRRAGIDDRMSSQIFDRYLQFLDGQRSYFLASDIAEFERY
                     RMRFDDMIRTGEIEPAYVIFARYQARNRDRVQHALVQLAKEPDWTIDESFGFDRSEAA
                     WPATEAEMNEIWRKRVKNDALGLMLAGKTWEEAADTLRKRYERVQKRVDQVTAEDVFE
                     NLMNAYARTFDPHSSYFSPRNSEEYRIQMSLNYEGIGASLQLIDEFVTVMNVIEGGPA
                     AVAGTLKPTDRIVGVAQGADGAFTDVVGWRIDDVVQLIRGKGGTLVRLRVLPGGAAPG
                     TPERTLEFTRNKVTLEAQAAKKSVHTLARGGHTYRIGVINVPGFYQDIAAQNAGDDDY
                     RSTTRDVRRLIAELQDEGIDGLVMDLRGNGGGYLPEATALTGLFIDRGPVVQLRDSGG
                     RTEVLDDPEPGVVYGGPLAVLVDRFSASASEIFAGAIQDYRRGVVLGQQTFGKGTVQN
                     LVPLDGYARKPVNGQLTVTIGKFYRITGESTQLRGVSPDVPLASLVSVEDVGESALDE
                     ALPWDRIAGVPYRSAAGSGFTPATLADEEGARAARATGRYDWGA"
BASE COUNT         1247 a         2652 c         2612 g         1174 t
ORIGIN      
        1 ctgccgaccg gcatgtcgta caccctcaac gtcgagccat cgccctgccc ccagagcgag
       61 cgctcggaca tcgtcggttg cagcttgcgg cgcacggcga gcaggctccc ctgctcatcg
      121 atgaaggcct ggctgttgta cagggtcccg ccgtcgcgct ccgacagccc gagcacgacg
      181 ttgacacggt gctgcgcgca cgcggcctgc acggcatcga gctcaccggc ggcgacgtcc
      241 accgactgcg ccgcgtactc gagcatcacc tcgcgctgcg cgagcggcgg atagagcccc
      301 agccagtacg catagcccgg cacgaaagtc tccgggaagc cgagcaggcg cacattgccg
      361 cggccggctt cctggatcag cgcacaggtc ttgtcgagtg tggccgcctt gtcgagcagg
      421 atcggtgcgg catgcgccgc ggcagccagg aaccgggcgg tcactcgcga cccgcgtcgc
      481 cgcgcacgat ctcgatcagg gcattgaggg catcggtgac cgtgaagatc ccgaggaact
      541 cgccgcgctc atcgttgacg attgcgctgc cgagtttcct gtcggacatg gcgaaggcca
      601 cctcgtccag cggctgcgcg gcgctcacgg tgagcaggtc ggtcgccatg aggtcggccg
      661 cgctcacctg tatgcggtgc ccggcgccga ggcccagcgc gacgcgcacg tcgcgatcgc
      721 tgacgagccc gacgatgcgt ccgtcacgaa cgatgggcag gtggcgcacg ccatgctcct
      781 gcatgagctc gcgcagctcg tcgacaggca gatccccgct tgcggtgatc gggttcggcg
      841 tggtgaattc ctccaccggc acgttcagtc tcttcatgag gactctccct ggtctgctgc
      901 agaattatgg ctccgatgcg caggcgggcc gatacgggct ttaccctatg gtctgcgtat
      961 ccgcccggcc gacccgcatc accaggggcc agctcacgag gcgcgccccg ctcccccagt
     1021 gcggcgcgag ttgccgctcg agcttcgcga ccggatcgcg cccgcgcgca gcggcgtagc
     1081 gcgccgtcgc cgaccaggtg cgcaggtaac ccgcgaactg cggcagcgtc cagtgcacct
     1141 gcatcgcgaa gcgcggagct tcgatcggcg tgaacgggaa cggcaggtcg cgatagccgc
     1201 gctcgatgtg cgcgcgctcg ggcggccagt acggacccaa tgcaccatgg tagagctcga
     1261 gcatcagggc atcgatcggg cccgcatcga cgctcgtgag cccatagcac cactcggcga
     1321 tcacgccgcg cggcacgagc acgcgccgag ccgcggcgtg aaatgcgtcg acatcgaacc
     1381 agtgcagggc ctgcgcgacg accaccaggt cgacgcagcg cgccgcgagc cccggcgcct
     1441 ccgccggtgc gacgcggtat tcgatgcgcg tgtgcggcgc ggcctgcgcg atctgcgccg
     1501 cgctcgcatc agtggcgatc acgcgcttga agtgtcgcgc caggtccacg gccgcctggc
     1561 cgctgccggt cgcgcagtcc caggcgagct cgcgcgcggg cgccgctgca gcgagccagt
     1621 cgaacagcgc cgcgggatag ctcgggcggt gactcgcgta gccgcgcgac acgtcgcgaa
     1681 aatgatccgc gaacgccacc ttcaggccga cacgaacacc ttgaagccgc cccagaacat
     1741 gcgactgccg tcgaacggca gcttcttcgg atccatcatc gaggcgaggc gcgggtccct
     1801 catcaccttg tcgttgacga tgtcgcgctg ccggcgcgat ttgtagacca cccacgagaa
     1861 catcacgacc tcaccgggct tcagcttcac gctctgcggg aaggacgtga ccttgcccgg
     1921 cttgacgtca tcggcgatgc actcgtggta ctcgagtgcg ccgtggtcgc gccagacctt
     1981 ggcggctttg cgcgccatcg cgcgataggc tgcgagcttg cggcgcggaa ccggggtaac
     2041 gaagccatcc acatacctgc ccatgatcga tttcctcctc tcgccgtggt tcatgccctc
     2101 agggcgccgg agtcgggcgg ccgtcgagcg tcatgatcgc gccgtacagg tccggacgac
     2161 ggtcgcgaaa tacgaaccag ttgttgcgca gcgcttcgat ctcggtgaga tcgaagctcg
     2221 cggtgagcac ggcgtcgccc tcctcgcccg cttcgaccag cttagccccg gtcggatcgg
     2281 cgatgaacga cgagccgtag aagcggatcc acagtccctc gggatcctgc cgcgagcgct
     2341 cgaaaccgta acggttcgac gcgatcagcg gcatgatgtt cgcggcggca tgcccctgct
     2401 gcgtgcgctg ccagtgctcg cgcgagttga ggggcagcgc gggcggcggt tcgctgccga
     2461 tcgcggtcgg gtagagcagc agctccgcac ccatcagcgc catgcagcgc gcggtttccg
     2521 ggaaccactg gtcccagcag atgccgacgc cgagccgcgc atggcgcgtg ttccacacct
     2581 tgaagccgct gtcgccggga gaaaagtagt tcttctcctg gtagccggga ccgttcggga
     2641 tgtgcgcctt gcggtacacg ccgagcaccg agccgtcggc atcgatgatc gcgatcgaat
     2701 tgaagaacgc ctggccggcg cgctcgaaga agctgatcgg catgaccacg cccagttcgc
     2761 gcgcgatcgg cgcgaagcgc cgtaccgccg cattgtcctc gagcgtggtc gcaagctgca
     2821 gatggcgcga gtcctgctcg atgcagaaat acggcgtctc gaacagctcc tgcagcagga
     2881 tcacctgcgc gccgcgctcg gcggcctcgc gcgtgagccg ctcagcgaca gcgaggttgg
     2941 cagcaagatc ccagctgcag gcgaactgcg tggcggcgac cgtgacggtg cggggtggcg
     3001 gcgagctcat ggcgcgcagc ttagggccag aaccgccagc aggccagacg gtgtccggcc
     3061 ctcagtgcat ggtggcgtcg gcgcccggaa agagtgcctg gtcgagcgcc ttgacgtaga
     3121 tctgctcgtc ggccggcccc ggcacgagga cctgccggtt gaacagcagg ttcgggatcg
     3181 cggtcacacc gagcagcagc aggcgccgct cctcgtcgat gacctcggca tagcccgcct
     3241 gcgggtcgcg cagcgcggtg aggaccgcgt cgctcagccc gcattcggcg ccgatcgcgg
     3301 cgagcacctc gatgtcgccg atgtcgcggc cctgctcgaa ataggcgcgg aagatcgcat
     3361 cgagcacggt gccgtgcagc ccctcggact cggccagctt caccagtcga tgcgccgcgg
     3421 tggtatccgg cacgccggcg agcttcccga agttgaagcg gatgccgaga ctctcgccgg
     3481 cctccgccag gctcgactcg gcaaactcga cgctgacgcc cttcggcaac cgggatgcga
     3541 gcagctcacg ccagttcgtg ggcgcgccag cctcgggccg cagcagccgg aacgggtgcc
     3601 agcgcagttc gcgcacggtg acgccgtgca ggtgatcgag cgcgcgcgcg agacggcggg
     3661 tgccgagata cgaccaggga caggcgaaat cgccgaccag atcgatgtcc agggtgccac
     3721 gcggcggggc aatttcgggc agttgcgtgc tcatgaggcg aggatggggg ccccggagcg
     3781 catttcaagt cagcggcgcc gggcgctcgc ggagacttcg cgcgtgatgc ggatggcggt
     3841 ttcgagcgca cgcagcccat cctcgccgct cacgaccggc ggcttgccat ggcgcgcgca
     3901 gtcgaggaac gactcgatct cggcgcgcag cgcatcgccc tgctcgaagc tctgctcctc
     3961 gatcgccacc ggcagctgcc cctccgccgg ggtgccctcg cgcttcctga tcacggtcag
     4021 gatgcgctgt tgcaggtcga tcgacaggta ggcatcgtcc tcgaacaggc gcagcttgcg
     4081 ctcggtcttc aggctcacgc ggctcgccgt ggcgttggcg atacagccat tctcgaagcg
     4141 gatgcgtgca ttggcgatgt cgatctcgtc ggagaacacg ggcgcgccga ccgcctccag
     4201 cgccgcgatc gggctgccga cgatcgactg cacgatgtcg atgtcgtgga tcatcaggtc
     4261 gagcacgaca ttgacgtcgg tgccgcgcgc ccggtatggc gcgagacggt ggcattcgat
     4321 gaaacgcggc ctggacaggt agggctccgc ggcgagcacc gccggattga aacgctcgag
     4381 atgcccgacc tgcagcacgc ggcccgccgc ctgcgcggcc tcgatcagcg cgcgcgcctg
     4441 ctccacggtt tcggtgaccg gcttctcgac gagaacgtgc gccccggcgc gcaggaaatc
     4501 gagcgcgatc gcgccgtggc tcgccgtcgg agtcacgata ctcacggcgt cgacttcgcc
     4561 cagcagctcg tgatggctgg cgagcggctt cgcaccggtc tccaccgcca cgcggtggcg
     4621 tgcctcctcg tcggcatcga cgaccgccac gagccggctg ccgggcacct gcgcatattt
     4681 ctgtgcgtgg aatctcccga gatacccgac gccgatcacg gcggtgcgca tgtcctgcat
     4741 caggctatta tgcccgcctg atgaccagcc cgatcgccgc atatcgcgcc cgctcgcccc
     4801 tcgcccgcga ggcgctgctc gccctcatcg ccctgatcgt gggcgtcacg ctactgccac
     4861 ttgcggttta ctacaccggc gtcgagatcc tgggacccta ctccgagggc gggctgtggc
     4921 gcttctgggg cgacttcttc aagggtctcg cccggggcgg cctgtcgtgg tggctcatag
     4981 cgctgggccc ctatgccctg ctgctgtttg cccgtggggc gcgcggcgcc ttgcgctgga
     5041 ccgcgcgata atgtgaccca ggtcacggtt tgggccctgg gtagccaggg aagggcccgc
     5101 aatgcgacac tgtcccgtga cgagttcgat tttgaccggg gatgagatga agaacatatc
     5161 cgaacgggcg cccgagacgc ctgcggaggc cgtggccgcg cgcgacaccg ggcgcagcga
     5221 gaacaccctg aagcgcgtga gcctcgcgtt gctgcatgag atcgcctccg atgaccgcga
     5281 gcgcggcaac gacccgtaca actccaacgc cggcaacgcg caggccgcca tctggggccg
     5341 ccagggccga cgacgctagg ctcgcccgtt ggatggcgcc ggcgggccca gggcccggcg
     5401 cgaccgatgg tgcccgtgag gggaaccccg gggcacccgg actgatcgaa gcttcagtgg
     5461 acactcaccg gggccagtac acccagccgg caagcagcag ccccgcgatc agcgcagaat
     5521 gggcggcgag acgccatccg cgcgcgcgga tttcgccgat gaaactcgcg gcggccagca
     5581 cggtgagcag gagcgcgagc gcgagcgcgc gcagcagcgg cacacgttcc gccgccagcc
     5641 gatcggcgta ctcgggcagc agcaggaaca cgatgcccgt gagaagcagc gcgatgctga
     5701 tcgaaagagt cgatcccatc acgattccga tgagcatggc gagcggtcgc atattctcca
     5761 ccgcgggatg ccgcggtgcg gacttgccgc ccccggcagc gcgttctagc ctagactgac
     5821 tggattgtaa ccgagccgca gacatcgagc cccgaatgca gacgacccat cgcctgagcg
     5881 ccggattcac cgcctccgca atcatcgcgg tgatgctggc gctcgtcagc tcatcgcagg
     5941 ccccggccag cacctccgcg ctgccgcccg gggcgattgc gccgggcgaa cggcatcgtc
     6001 tggtcgcgcg ccgcgtcggc atgatcctcg aggacacgca ctaccgccgc gccggcatcg
     6061 acgatcgcat gtcgtcgcag atcttcgatc gctatctgca gttcctcgac ggccagcgca
     6121 gctacttcct cgcgagcgac atcgcggagt tcgagcgcta ccgcatgcgt ttcgacgaca
     6181 tgatccgcac cggcgagatc gagcctgcct acgtgatctt cgcgcgctac caggcgcgca
     6241 accgcgatcg cgtgcagcat gcgctggtac agctcgcgaa ggagccggac tggaccatcg
     6301 acgaatcctt cgggttcgac cgctccgagg ccgcctggcc ggccaccgag gcggagatga
     6361 acgagatctg gcgcaagcgc gtcaagaacg atgcgctcgg cctgatgctc gcgggcaaga
     6421 cctgggagga agcggccgac acgctgcgca agcgctacga gcgcgtgcag aagcgcgtcg
     6481 accaggtgac cgccgaagac gtgttcgaga acctgatgaa tgcgtacgcg cgcaccttcg
     6541 atccgcattc gagctacttc tcgccgcgca attccgagga ataccgcatc cagatgagcc
     6601 tcaactacga gggcatcggc gcctcgctgc agttgatcga cgagttcgtc acggtgatga
     6661 acgtcatcga gggcggcccg gcggcggtcg ccggcaccct caagcccacc gaccgtatcg
     6721 tcggcgtcgc gcagggtgcg gacggcgcgt tcaccgatgt ggtgggctgg cgcatcgacg
     6781 acgtggtgca gctgatccgc ggcaagggcg gcacgctcgt gcgcctgcgc gtgctgccgg
     6841 gcggcgccgc gccgggcaca ccggagcgga cccttgaatt cacgcgcaac aaggtcacgc
     6901 tcgaggcgca ggccgcgaag aagtcggtgc atacgctcgc gcgtggcggg cacacctatc
     6961 gcatcggtgt gatcaacgtg ccgggcttct accaggacat tgccgcacag aatgcgggtg
     7021 acgacgacta tcgcagcacc acccgcgacg tgcgccggct gatcgcggaa ctgcaggacg
     7081 aaggcatcga cggcctggtg atggacctgc gcggcaacgg gggcggttac ctgcccgagg
     7141 ccaccgccct caccggcctc ttcatagacc gcggcccggt cgtgcagctg cgcgattccg
     7201 gcgggcgcac cgaagtgctc gacgatccgg agccgggcgt cgtctacggc gggccgctcg
     7261 ccgtgctggt cgatcgcttc agtgcatcgg cgtcggagat tttcgccggc gcaatccagg
     7321 attaccggcg cggcgtggtg ctcggccagc agaccttcgg caagggcacc gtgcagaacc
     7381 tcgtgccgct cgacggctat gcgcgcaaac cggtcaacgg ccagctcacc gtcaccatcg
     7441 gcaagttcta ccgcatcacg ggcgagagca cgcagctgcg cggcgtctcg cccgacgtgc
     7501 cgctcgcgtc actggtcagt gtcgaggatg tcggtgagag cgctctcgac gaagcgctgc
     7561 cctgggaccg catcgccggt gtgccctatc gcagcgccgc cggctccggt ttcacgccgg
     7621 cgaccctcgc cgacgaggaa ggtgcgcgcg ccgcgcgtgc tacggggcgt tacgattggg
     7681 gtgct
//
LOCUS       DAOQVY010000115         7637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_10583_length_7637_cov_5.840280, whole genome shotgun sequence.
ACCESSION   DAOQVY010000115 DAOQVY010000000
VERSION     DAOQVY010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10583_length_7637_cov_5.840280"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PKN91_10185"
     CDS             complement(<1..312)
                     /locus_tag="PKN91_10185"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNS28477.1"
                     /translation="MPGSAHSIDLDVQAADIDEYSHVNNAVYLTWLDRAAWSHSAALG
                     MPLERCIALRRGMAALRTEIDYERAAIAGDRIRVSTWIADCDGRLRCTRHFVVSRAAD
                     GA"
     gene            complement(315..797)
                     /locus_tag="PKN91_10190"
     CDS             complement(315..797)
                     /locus_tag="PKN91_10190"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNS28478.1"
                     /translation="MAHPLYPPGSGVAPPEVLKSLSGLEFMQRMVAGALPPPPIAITL
                     DFHVVAVERGVVTFEGRPGANVYNPLGLIHGGYAATLLDSCMGCAVMTHVEAGMGYVT
                     LELKVSYLRAMTHTTGAVRAIGRALHVGRRSGFAEGEIVDASGRRIAHGTTTCLVYPL
                     "
     gene            complement(815..2110)
                     /gene="purD"
                     /locus_tag="PKN91_10195"
     CDS             complement(815..2110)
                     /gene="purD"
                     /locus_tag="PKN91_10195"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNS28479.1"
                     /translation="MKVLVVGSGGREHALAWKCAQSARVTQVLVAPGNAGTALEPKLR
                     NVAVAADDIEALVALARSEQVALTIIGPEAPLVLGVVDAFSAAGLRCFGPHRAPAQLE
                     GSKAFTKEFLRRHRIPTAAYATFTKASFDPAWVRAQRAPIVVKASGLAAGKGVVIAAS
                     ADEAIATAQAMFDGRFGAAGEEVVIEEFLAGEEASFIVMADGEHILPLATSQDHKRLG
                     DADTGPNTGGMGAYSPAPVVTPALHDRIMREVIEPTIRGLAADGMPYTGFLYAGIMIA
                     PDGTPNVLEFNCRFGDPETQPILARLRSDLVDLCDAALERRLDGVTAEWDPRAAVGVV
                     LAAEGYPDAVRKGDPIEGLARAAQLPGKVFHAGTERRGEAIVTNGGRVLCAVGLGATV
                     AAAQRDAYALVGAIRWPGMQYRRDIGYRAIAREQAGAAH"
     gene            complement(2127..3686)
                     /gene="purH"
                     /locus_tag="PKN91_10200"
     CDS             complement(2127..3686)
                     /gene="purH"
                     /locus_tag="PKN91_10200"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNS28480.1"
                     /translation="MLVEIRRALLSVSDKTGLVDLARALARRDIEILSTGGTARLLRE
                     HGVTVTEVSAYTGFPEIMDGRVKTLHPRIHGGLLGRRGVDDAVMQLHDIPPIDLLVVN
                     LYPFAETVARPGCTYDEAIENIDIGGPAMLRAAAKNHHSVTVVVDPADYAGLLAELDA
                     NDGSSSIDTRSQFAAKAFAHTARYDTMVSNYLLRREQAERFPGTLPLVYDKLRDLRYG
                     ENPHQGGAFYREVAARGASIATATFVQGKELSYNNILDADTAIECVRQFTPPACVIVK
                     HANPCGVATADGPAAAYDKAYRTDPTSAFGGIIAFNRELDAATAQAIVERQFVEVIAA
                     PAIAPAALAVLATKANIRVLAVGDLGRSPGNDLEYRSVGGGLLVQARDQGSITAAGLR
                     VVTKREPTEAQVADLLFAWVVCKHVKSNAIVFARDHMTIGVGAGQMSRVYSTRVAAMK
                     AADEKLEVRGAVMASDAFFPFRDGIDVAAQYGIAAVIQPGGSKRDSEVIAAADEHGLA
                     MVFTGMRHFRH"
     gene            complement(3705..3995)
                     /gene="fis"
                     /locus_tag="PKN91_10205"
     CDS             complement(3705..3995)
                     /gene="fis"
                     /locus_tag="PKN91_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004751481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HNS28481.1"
                     /translation="MPAKKTQRPRAEPIARANGRSLPLRDHAEQALSDYFATLNGHRP
                     ARLYDMVLREVEEPLFRVVLDYAEGNQSRAAGILGITRGTLRKKLRQFGLSA"
     gene            complement(4030..5016)
                     /gene="dusB"
                     /locus_tag="PKN91_10210"
     CDS             complement(4030..5016)
                     /gene="dusB"
                     /locus_tag="PKN91_10210"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNS28482.1"
                     /translation="MQIGPYALSAPVALAPMAGVTDRPFRVLCRRLGAGLAASEMLTA
                     DTRLWRSRKSQRRMNHEGEPSPRVVQLAGADPQALAAAARLNVDLGAEVIDINMGCPA
                     KKVFNRQCGSALLADEPLVARILEAVVAAVTVPVTLKIRTGVDPRHRNGVAIARIAAA
                     CGVVALAVHGRTRADHYAGTAEYATIRDIKRAVSIPVFANGDIDCAQKAREVLDFTCA
                     DGVMLGRAAHGAPWIFRDVNEFMSSGRLPAALARAEVRDIIRTHLTDLYHFYGEVDGV
                     RFARKHLGWYFAGLLGTQDDRRGLMSQETSARQFAYASAWLDRWVDGTANAA"
     gene            complement(5035..6210)
                     /locus_tag="PKN91_10215"
     CDS             complement(5035..6210)
                     /locus_tag="PKN91_10215"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HNS28483.1"
                     /translation="MFTVCPKCALTLAVTAADLRVGQGYVRCGRCASVFNALVALHDE
                     AQRDEADPSATTDRRAATPSPPPPPLMMELPVTADSAGEAEPTPDTMEFALGSADLAQ
                     IFVAPEIAEGEAASGTFEAIVLEGELVATDTETETETETEADVASDALTRELEDAEAE
                     LERIAQIARDVAADPGSATQQSEALQVSMAAPVGDRFAEHYSVRAEDIDAFEEPQRPP
                     RVELWYGIAAGVLALLLVAQVVHRYRNELALNPALNGPLASLYDALGAPLAPRWDPAA
                     LDVRQLGAIAEGDASGQLLVRASIRNEASRAQPMPLLRLTLQDRYGKRLASRDLTPRE
                     YLGERAGARDFLAAGQRVDAEVAVLDPGSNAVGFEVDACLPNGQGGVACAGDSRAAR"
     gene            complement(6246..7121)
                     /gene="prmA"
                     /locus_tag="PKN91_10220"
     CDS             complement(6246..7121)
                     /gene="prmA"
                     /locus_tag="PKN91_10220"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNS28484.1"
                     /translation="MPFLSITIDLDDRDAEATEAACFAAGALAVTLTDRRDDAVLEPA
                     PGEIRLWPATRLEATFAQDDASADTLVSLAAALGVPVSRLTVKTVADRAWEREWLTDF
                     HAMRFGRRLHVCPTHERVADPHAVVVTLDPGLAFGTGTHPTTALCLEWLDAELRPGET
                     VIDFGCGSGILAIAAARLGAARVDAHDIDPQALIATRDNAQANAVADRVVTHAGTDTL
                     PAGADVLLANILAGPLIELAPRLAALVRPQGRLVLAGLLTSQAEEVAAAYRPWFDMQT
                     AGERETWTLLAGIRR"
     gene            complement(7137..>7637)
                     /locus_tag="PKN91_10225"
     CDS             complement(7137..>7637)
                     /locus_tag="PKN91_10225"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650484.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNS28485.1"
                     /translation="GEFYFIEMNTRIQVEHPVTETITGIDLVKAQLLIAAGEKLPYVQ
                     KDIEFRGHAIEARINAEDAKTFMPSPGPVKLWHAPGGPGIRVDSHLYSGYNVPPNYDS
                     LIGKVIAYGDTRDTAIARMKMALAEMVVEGIKTNSALHREIFNHAAFRSGGLDIHYLE
                     RRLGLK"
BASE COUNT         1154 a         2545 c         2763 g         1175 t
ORIGIN      
        1 ggcgccatcg gcggcgcggc tgaccacgaa atggcgcgtg cagcgcagcc tgccgtcgca
       61 atccgcgatc caggtcgaga cgcggatgcg atcgccggcg atcgccgcgc gttcgtaatc
      121 gatctcggtg cgcagggccg ccatgccccg gcgcagcgcg atgcaacgct cgagcggcat
      181 gccgagcgcg gccgaatgac tccacgcggc acgatcgagc caggtgaggt agaccgcgtt
      241 gttgacgtgc gagtactcgt cgatgtcggc ggcctgcacg tcgaggtcga tcgaatgtgc
      301 agaccccggc atcgtcatag cgggtagacg aggcaggtgg tcgtgccgtg cgcgatgcgc
      361 cggccggacg catcgacgat ctcgccctcg gcgaagccgc tgcgccgccc gacgtgcagg
      421 gcgcggccga tcgcgcgcac agcacctgtg gtgtgcgtca tcgcgcgcag gtagctcacc
      481 ttcagctcga gggtgacata gcccatgccg gcctcgacgt gggtcatgac cgcgcagccc
      541 atgcacgaat cgagcagcgt cgcggcgtag ccgccatgga tcaggccgag cggattgtag
      601 acattggccc cgggacgccc ctcgaaggtc acgacgccac gctcgacagc cacgacatgg
      661 aagtcgaggg tgatcgcgat cggcggcggt ggaagcgcgc ctgcgaccat gcgctgcatg
      721 aattcgaggc cggaaagcga cttgaggact tccggaggag cgacgccact gcccggcggg
      781 tagagtgggt gggccatcca aatagtctac ccgatcagtg cgcagcgccc gcctgctcgc
      841 gcgcgatggc gcggtagccg atgtcgcggc ggtactgcat gccgggccag cggatcgccc
      901 cgacgagcgc gtaggcatcg cgctgggcgg ccgcgacggt agcgccgagg cccaccgcgc
      961 acagcacgcg cccgccgttg gtcacgatcg cctcgccgcg gcgttcggtg cccgcatgaa
     1021 acaccttgcc gggcaactgc gccgcgcgcg cgaggccctc gatcggatcg cctttgcgga
     1081 ccgcatcggg atagccctcg gcggcgagca cgacgcccac cgcggcgcgc ggatcccatt
     1141 cggcggtcac gccgtcgagg cggcgctcga gcgcagcgtc gcacaggtcg accaggtccg
     1201 agcgcaggcg cgcgaggatc ggctgcgtct ccgggtcacc gaagcggcag ttgaactcga
     1261 ggacattggg cgtgccatcg ggcgcgatca tgatgccggc gtacaggaag cccgtgtagg
     1321 gcatgccgtc ggcggccagg ccgcggatgg tcggctcgat cacctcgcgc atgatgcgat
     1381 cgtggagcgc cggcgtgacg accggggcag gcgaatacgc gcccatgccg ccggtattcg
     1441 ggccggtgtc ggcatcgccc aggcgcttgt ggtcctgcga ggtggcgagc ggcaggatgt
     1501 gctcgccatc ggccatcacg atgaagctcg cttcctcgcc cgcgaggaat tcctcgatca
     1561 cgacctcttc acccgcggcg ccgaagcgcc cgtcgaacat ggcctgcgcg gtggcgatcg
     1621 cctcatccgc gctcgcggcg atcacgacgc ccttgccggc ggcgagcccg ctcgccttga
     1681 cgacgatcgg cgcgcgctgc gcgcgcaccc aggccggatc gaaactggcc ttcgtgaagg
     1741 tggcgtaggc ggcggtcgga atgcggtgac ggcgcaggaa ttccttggtg aaggccttcg
     1801 agccttcgag ctgcgccggc gcgcggtgcg gcccgaaaca tcgcaggccc gcggcgctga
     1861 acgcatcgac gacgccgagc acgagcggcg cctcggggcc gatgatggtg agagccacct
     1921 gttcgctgcg cgcgagcgcg accagcgctt cgatgtcgtc ggcggccact gcgacattgc
     1981 gcagcttcgg ctcgagtgcc gtgcccgcat tgccgggcgc caccagcacc tgcgtgacgc
     2041 gcgcggactg cgcgcatttc cacgcgagcg cgtgctcgcg accgcccgaa ccgacgacca
     2101 ggactttcat gggcgggcgc cccgcatcaa tggcggaagt ggcgcatgcc ggtgaatacc
     2161 atggcaaggc cgtgctcgtc ggccgcggcg atgacctcgc tgtcgcgctt cgagccgccc
     2221 ggctggatca ccgcggcgat gccgtactgc gccgcgacgt cgatgccgtc gcgaaacggg
     2281 aagaaggcgt ccgaggccat cacggcgcca cgcacttcga gcttctcgtc cgctgccttc
     2341 atcgcggcga cgcgggtcga atacacgcgg ctcatctgcc cggcgccgac gccgatcgtc
     2401 atgtggtcgc gggcgaacac gatcgcgttc gatttcacgt gcttgcagac gacccacgcg
     2461 aacagcaggt cggcgacctg cgcctcggtc ggctcgcgct tcgtgacgac acgcaggcct
     2521 gcggcggtaa tgctgccctg gtcgcgcgcc tgcacgagca ggccaccgcc gacactgcgg
     2581 tattccagat cgttgccggg cgagcggccg agatcgccca ccgcgagtac gcgaatattg
     2641 gccttggtgg cgagcacggc gagggcggcg ggcgcaatgg ccggcgcggc gatgacttcg
     2701 acgaactgac gctcgacgat cgcctgcgcc gtggcggcat cgagttcgcg gttgaaggcg
     2761 atgatgccgc cgaaggccga tgtcgggtcg gtgcgatagg ccttgtcgta ggccgccgcc
     2821 gggccatcgg cggtggcgac gccgcagggg ttcgcgtgct tgacgatcac gcaggccggc
     2881 ggcgtgaact ggcgtacgca ctcgatggcg gtgtcggcgt caaggatgtt gttgtaggac
     2941 agttccttgc cctgcacgaa ggtcgcggtg gcgatcgagg cgccgcgcgc ggccacttcg
     3001 cggtagaagg cgccgccctg gtgcgggttc tcgccgtagc gcaggtcgcg cagcttgtcg
     3061 tagacgagcg gcagggtgcc ggggaagcgc tcggcctgct cgcgacgcag caggtagttc
     3121 gacaccatgg tgtcgtagcg cgcggtgtgc gcgaaagcct tggcagcgaa ctgcgagcgc
     3181 gtatcgatgc tcgagctgcc gtcgtttgca tcgagctcgg cgaggaggcc cgcgtagtcg
     3241 gcagggtcga cgaccacggt gaccgagtga tgattcttgg cggccgcccg cagcatcgcg
     3301 gggccaccga tgtcgatgtt ctcgatcgcc tcgtcgtagg tgcaacccgg acgggcgacg
     3361 gtttcggcga acggatagag gttgacgacc agcaggtcga tcggcgggat gtcgtgcagc
     3421 tgcatgacgg cgtcgtcgac gccacgccgc ccgagcaggc cgccgtggat gcgcgggtgc
     3481 agcgtcttga cgcgcccgtc catgatctcg ggaaagcccg tgtaggccga cacttcggtc
     3541 accgtgacgc cgtgctcgcg cagcagtcgt gcggtgccgc cggtggagag gatctcgatg
     3601 tcgcggcgcg ccaacgcgcg ggcgaggtcc acgagcccgg tcttgtccga gacggaaagc
     3661 agcgccctgc ggatctcaac gagcatgatg cggttgcggc cggctcaggc cgacagcccg
     3721 aactggcgca gtttcttgcg cagggtgccg cgggtgatgc cgaggatgcc ggcggcgcgg
     3781 ctctggttgc cctccgcgta gtcgagcacg acccggaaca gcggctcctc gacctcgcgc
     3841 agcaccatgt cgtacaggcg cgccggacga tggccgttca gcgtggcgaa gtaatcggac
     3901 aacgcctgtt cggcatggtc gcgcaggggc aagctcctgc cgttcgcgcg cgcgatgggt
     3961 tcggcgcgcg gcctctgggt tttcttcgca ggcatcagca gtccccgacg cagcaactcg
     4021 ttgttgtgat caggccgcgt tcgcggtccc gtcgacccag cggtcgagcc atgcactcgc
     4081 atatgcaaac tgtctcgcgg aagtttcctg ggacatcaac ccgcgccgat cgtcctgtgt
     4141 gccgagcagc cctgcgaaat accagccgag gtgtttgcgc gcgaaacgga cgccgtcgac
     4201 ttccccatag aagtggtaca gatccgtcag gtgcgtgcga atgatatcac gcacctcggc
     4261 gcgcgcgagc gcagcgggca gtctgccgct cgacataaat tcgttgacat cgcgaaaaat
     4321 ccacggcgct ccgtgcgccg cgcggccgag cattacgccg tccgcgcagg tgaaatcaag
     4381 tacctcgcgc gccttttgcg cgcaatcaat atcgccattg gcgaacaccg ggatcgacac
     4441 cgcccgcttg atatcgcgaa tcgttgcgta ttccgccgtg cccgcgtagt ggtccgcgcg
     4501 cgtgcgcccg tgcaccgcga gcgcgacgac gccgcaggcg gccgcgatgc gcgcgatggc
     4561 gacgccgttg cgatgtcgcg gatccactcc ggtgcggatc ttgagcgtca ccggcacggt
     4621 cacggcagcg accaccgcct cgaggatgcg cgcgaccagc ggctcgtcgg cgagcagggc
     4681 cgaaccgcac tggcggttga agaccttctt cgccgggcag cccatgttga tgtcgatgac
     4741 ctcggcaccg agatcgacat tgaggcgcgc ggccgcggcg agcgcctgcg ggtcggcgcc
     4801 cgccaactgc accacgcggg gcgagggttc accttcgtgg ttcatgcggc gctgcgactt
     4861 gcggctgcgc cacaggcgcg tgtcggcggt gagcatttcc gatgcggcga gacccgcgcc
     4921 gagacggcga cacagcacgc ggaacgggcg atcggtgacg cccgccatcg gcgcgagcgc
     4981 aacgggtgcg gacagggcgt aggggccgat ctgcatgacg gcggcgggcc gggttcagcg
     5041 ggccgcgcgc gaatcgccgg cgcaggcgac tccgccctgg ccattcggca ggcaggcgtc
     5101 gacctcgaag ccgacggcat tgctgccggg gtcgaggacc gcgacctcgg cgtcgacgcg
     5161 ctggccggcg gccaggaaat cgcgcgcgcc ggcgcgctcg ccgagatact cgcgcggcgt
     5221 caggtcgcgg ctggcgagcc gcttgccata gcggtcctgc agcgtgaggc gcagcagcgg
     5281 catgggctgt gcgcgcgagg cctcgttgcg gatgctggcg cgcaccagca attgcccgct
     5341 ggcgtcgcct tcggcaatcg cgccgagctg gcgcacgtcg agcgccgccg gatcccagcg
     5401 cggcgcaagg ggtgcgccga gcgcatcgta aagcgatgcg agcgggccat tgagcgcggg
     5461 attcagggcg agctcgttgc gatagcgatg cacgacctgc gccacgagca gcaaggcgag
     5521 gacgcccgct gcgatgccgt accacagttc gacgcgcggt ggccgttgcg gctcctcgaa
     5581 ggcgtcgatg tcctcggcgc gcacggaata atgctcggcg aaccggtcac ccaccggtgc
     5641 ggccatggag acctgcagcg cttcggactg ctgcgtcgcc gagcccgggt cggcggccac
     5701 gtcgcgggcg atctgcgcga tgcgctcgag ttcggcctcg gcgtcctcga gttcgcgcgt
     5761 gagcgcgtcg ctcgccacat cggcctcggt ctcggtctcg gtctcggtct cggtatcggt
     5821 ggcgacgagt tcgccttcga gcacgatcgc ctcgaaggtt cccgacgcgg cctcgccttc
     5881 ggcgatttcg ggcgcgacga agatctgcgc caggtccgcg ctgccgagcg cgaactccat
     5941 cgtgtcgggc gttggttcgg cctcgcccgc ggagtccgcc gtcaccggca gctccatcat
     6001 caggggcggc ggaggcggcg aaggggtggc agcgcgccgg tcggtcgtgg ccgacgggtc
     6061 cgcctcgtca cgttgcgctt cgtcgtgcag cgcaaccagc gcattgaaca cgctcgcgca
     6121 acggccgcag cgcacgtagc cctgcccgac gcgcaggtcc gccgcggtga cggcgagggt
     6181 cagggcacat ttcgggcaga ccgtgaacat gggcggatat tagcgcaggc tcgcgaccga
     6241 cgagctcagc gtctgatgcc ggcgagaagg gtccaggttt cgcgttcgcc ggcagtctgc
     6301 atatcgaacc acgggcggta ggccgcggcg acttcttcgg cctgcgatgt gagcaggccc
     6361 gccagcacga ggcgtccctg cggcctcacg agcgcggcga ggcgcggtgc gagctcgatc
     6421 aagggaccgg caaggatatt ggcgagcagc acatcggcgc cggccggcag cgtgtcagtg
     6481 ccggcgtgtg tcacgacgcg atcggcaacc gcgttggcct gcgcattgtc ccgcgtcgcg
     6541 atcagtgcct gcgggtcgat gtcatgggca tcgacccgtg ccgcgccgag ccgggctgcg
     6601 gcgatggcga ggatgcccga gccgcagcca aagtcgatca cggtctcgcc cggtcgcagc
     6661 tcggcgtcga gccattcgag gcacagggct gtcgtgggat gcgtgccggt gccgaaggcg
     6721 agtccgggat cgagcgtgac gacgacggca tgcgggtccg ccacgcgctc gtgtgtcggg
     6781 cagacgtgga gacggcgtcc gaagcgcatc gcatggaagt ccgtgagcca ctcgcgctcc
     6841 caggcgcgat cggccactgt cttcacggtg agccgcgaca ccggtacgcc gagcgcggcc
     6901 gccagtgaga cgagcgtgtc ggccgaggcg tcatcctgcg caaaggtcgc ctcgagccgc
     6961 gtcgcgggcc acaggcggat ctcgcccggc gccggctcga gcaccgcgtc atcgcgccgg
     7021 tcggtcagcg tgaccgcgag cgcaccggct gcgaagcacg cggcctccgt ggcctcggcg
     7081 tcgcgatcgt ccaggtcgat ggtgatggac agaaagggca tcgccctgcg gcccgcctac
     7141 ttcagcccga ggcgccgttc cagatagtgg atgtcgagcc cgcccgagcg gaatgccgcg
     7201 tggttgaaga tctcgcggtg cagggccgag ttggtcttga tcccctcgac caccatttcg
     7261 gcgagcgcca tcttcatgcg cgcgatcgcc gtgtcgcggg tgtcgccgta ggcgatgacc
     7321 ttgccgatca gcgagtcgta attcgggggc acgttgtagc ccgagtacag gtggctgtcg
     7381 acgcggatgc ccgggccgcc cggcgcgtgc cagagcttca ccgggcccgg cgagggcatg
     7441 aacgtcttgg cgtcttcggc attgatgcgc gcttcgatcg cgtggccccg gaactcgatg
     7501 tctttctgca cgtagggcag cttctcgccg gcggcgatca gcagctgcgc cttgacgaga
     7561 tcgatgcctg tgatcgtctc ggtgaccggg tgctcgacct ggatgcgcgt attcatttcg
     7621 atgaagtaga actcgcc
//
LOCUS       DAOQVY010000116         7609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_10644_length_7609_cov_5.073868, whole genome shotgun sequence.
ACCESSION   DAOQVY010000116 DAOQVY010000000
VERSION     DAOQVY010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7609
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10644_length_7609_cov_5.073868"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PKN91_10230"
     CDS             complement(<1..505)
                     /locus_tag="PKN91_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmF"
                     /protein_id="HNS28486.1"
                     /translation="MLPELGHFALVLALLLAALQAFFGLAGPAIGRGRWMAAVIPATA
                     GQAVMVVTAFACLVASFVGLDFSVAYVAQNANSALPLIYRIAAVWGAHEGSLLLWILL
                     LAMWTVAVAFGATRLPQHFSARVLGVLGIVSFGFLLFTLATSNPFLRLIPAAASVNSR
                     KPKLTMPS"
     gene            complement(511..954)
                     /gene="ccmE"
                     /locus_tag="PKN91_10235"
     CDS             complement(511..954)
                     /gene="ccmE"
                     /locus_tag="PKN91_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNS28487.1"
                     /translation="MTPRRRRLFLVLGILAGVAIAGVLTLQAFRENVMFFFDPSQIAK
                     GEAPAGERFRLGGMVEEGSVVRSPGSLQVQFVVTDFEQSVPVHYTGVLPDLFREGQGV
                     IAHGRLGADGVFTADEVLAKHDENYMPPEVQRSLKAVHPQGAPEG"
     gene            complement(951..1112)
                     /gene="ccmD"
                     /locus_tag="PKN91_10240"
     CDS             complement(951..1112)
                     /gene="ccmD"
                     /locus_tag="PKN91_10240"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HNS28488.1"
                     /translation="MSDFLSMGGYASSVWPSVLLTCAIIAWNVFAARRSFREARAEAR
                     RRLAMENAP"
     gene            complement(1109..1846)
                     /locus_tag="PKN91_10245"
     CDS             complement(1109..1846)
                     /locus_tag="PKN91_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HNS28489.1"
                     /translation="MDWTWFHRFGSPPHLYRLAGTLVPWFAWPAAILLAAGLYGGLVL
                     APPDYQQGDGFRIIYVHAPSAWLSLMAYSTMAVAAALGLIWRMKVAHAVAASCAPIGA
                     WFTVAALVTGMLWGKPMWGTYWAWDPRLTAELVLLFLYLGYMGLRAAIEDPQRADRAS
                     AVLAIVGVVNVPIVHYSVEWWNSIHQAPSVMKFGKPSISWPMLSPLLIMFVGFTLFFA
                     ALVLVRVRGEILRRERQSRWVAEVVAQ"
     gene            complement(1849..2526)
                     /gene="ccmB"
                     /locus_tag="PKN91_10250"
     CDS             complement(1849..2526)
                     /gene="ccmB"
                     /locus_tag="PKN91_10250"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNS28490.1"
                     /translation="MTGPWSAARLVVERDMRLAFRNRAQLLHPLLFFGIVTTLFPLAL
                     SPEASRLRDVAPGVLWVAALLSSLLAIEFLFRDDAQDGTLEQYALSGQSLTWLLFAKT
                     VSHWLLAGLPLALMSPVAAYGLGVPVGAVPGIVASVALGTVALSLLGAVTAGLTIGIR
                     RGGALLSLLTLPLAVPLLIFGAQATARAIAGTGIAGPMYFLAAFTVLGITLAPLAAAA
                     AVRISLE"
     gene            complement(2523..3152)
                     /gene="ccmA"
                     /locus_tag="PKN91_10255"
     CDS             complement(2523..3152)
                     /gene="ccmA"
                     /locus_tag="PKN91_10255"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HNS28491.1"
                     /translation="MSAHRITGDTLHVFRGERHVLRGLSFAATSGEYLEVSGANGAGK
                     TSLLRTIVGLVHAESGSVEWNGRSIHADPRAFHAQLAWLGHESPLKGDLSAVENLRYA
                     IGIRRPVDAAAIGRALDEVDASRFAERPVRTLSAGQRRRVALAGVLLAAAPLWVLDEP
                     ATHLDAAGHALVGHLVESRIKTGGIVIAAVHAALPVDEAHTRRLGLSTK"
     gene            complement(3191..4507)
                     /locus_tag="PKN91_10260"
     CDS             complement(3191..4507)
                     /locus_tag="PKN91_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS28492.1"
                     /translation="MQHFTGLLRGLLMALLIVVSIALWIVLPAAALLGLAILLAAWMG
                     LTRVGRQAAAITRIGIATLPQRLGASSVIIIGIAGVVGVLVALLALAGGLQQTLRGTG
                     NDDTAIVVRGGASAELDSGLSRESATLIGQAPGIARDASGQPIVSAEVVVVTNVPKKS
                     SGTDANVEIRGVGPRSWAVRPNVRIVEGRRFEPGLRELLVGQGARQQFAGLEPGETII
                     LNAQQWQIVGVFASGDAHESELIGDAETVAATYRRTGFQSVTVRLESPAAFDALKAAL
                     ASDPRLKVDARTTRDYYSRQSAGLSKVIRIVGIVIAAIMAIGAVFGALNTMYAAVNAR
                     AREVATLRAIGFGATPVLVSVVLEAMLLALLGGALGALIAWLLFHDYAVSTLGANFSQ
                     VVFRFDVSPPLLGTGVRWALAIGLIGGLLPAVRAARLPVTTALRES"
     gene            complement(4518..5681)
                     /locus_tag="PKN91_10265"
     CDS             complement(4518..5681)
                     /locus_tag="PKN91_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS28493.1"
                     /translation="MKYLHLIRAALMRRKTRTVLTILSVLAAFLLFGLLDGVRVAFNS
                     GGTLAGVDRLVVSSRFSIIQSLPESLVNRVAAVQGVRLVTWANWFGGYFREQKNQMII
                     FAIGPGYFDAYPDYGVTPAMAEALASTRSGVLVGKAVADRFGWKVGDKLPILGSIFPN
                     KATGETTWTFDIVGIYQAPLAANRALEQQILLNWKYFDEANAYGGGQVGWIVVKVADP
                     ADSARVAQAIDALSANSDHETKTQTEQEFNLSFAKQIGDIGLIVTAIIGAVFFTLMLL
                     TGNTMAQAVRERMPELATLKTIGFTDRQVLALVFGESVLLILIGGVFGLVLATLLMPI
                     VTEASGGMIQMPPVGWRSWGIGIALMIAVGAIVGILPAWRAMRLRVVDALAGR"
     gene            complement(5678..6361)
                     /locus_tag="PKN91_10270"
     CDS             complement(5678..6361)
                     /locus_tag="PKN91_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS28494.1"
                     /translation="MSALVEIRDVSKAYVRGRQTVEVLHHITLDIRKGDFLALMGPSG
                     SGKTTLLNLIGGLDSPSSGSISVDGTRIDQLGGTALAKWRAAHVGFIFQFYNLLPILT
                     AQKNVEVPLLLTKLSAAQRRQNAKIALQLVNLADRASHKPGELSGGQQQRVAIARAII
                     SDPTLLVCDEPTGDLDRQSAEEVLNLLQVLNREQGKTIIMVTHDPKAADHASHTLHLE
                     KGSLVESLP"
     gene            complement(6358..>7609)
                     /locus_tag="PKN91_10275"
     CDS             complement(6358..>7609)
                     /locus_tag="PKN91_10275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNS28495.1"
                     /translation="PMADTNENDKGRLLAQLRIDRTDESTTPRATWIAGGVTALLLLA
                     GAAFWWWSRATAVVDVELATAAAPAAAGSGASAVLQATGYVTARRQATVSAQITGKLT
                     EVLIEEGESVEAGQVLARLENSSQRAQLAETQAQLAAARARVGQYTSELAQARRDLER
                     QQDLLARQLVAEQAVEAARLQVDSLAAQLAAQQETVHLAEAQVRSAQVQLDYTTVRAP
                     FAGVVIAKAAQVGEIVSPLSAGGGFTRTGIGTLVDMNSLEIEVDVNEAYLNRVVPGGP
                     AQAVLDAYQDWTIPAHVIAIIPAADRGKATVKVRIGLDQKDSRILPDMGARVSLLAGE
                     TAVGATGGAGAGVLVPSSAIVRRDGADVVFLADGDRARAQPVRPGQRFGDLTQVDDVA
                     VGARVVRAPGEAIQDGTRIREEGT"
BASE COUNT         1304 a         2659 c         2560 g         1086 t
ORIGIN      
        1 tgctcggcat cgtcagtttc ggcttcctgc tgttcacgct cgccgccgcg ggaatgagtc
       61 tgaggaaagg attcgaggtg gcgagcgtga acagcaggaa gccgaaactg acgatgccga
      121 gcacgccgag cacgcgcgcg gagaagtgtt gcggcagacg cgtggcgccg aatgcgacgg
      181 cgaccgtcca catggcgagc agcaggatcc acagcagcag cgaaccctcg tgcgcgcccc
      241 agaccgcggc gatccggtag atgagcggca gcgcggagtt cgcgttctgc gccacgtagg
      301 ccaccgagaa atcaagaccc acgaagctcg cgacgaggca ggcgaatgcc gtcaccacca
      361 tgaccgcctg gcccgcggtg gcaggaatga cggccgccat ccagcgcccg cgcccgatcg
      421 cggggcccgc gaggccgaag aaggcctgca gcgcggcgag cagcagcgca agtaccaggg
      481 cgaagtggcc gagttcgggc agcatcggga tcagccctcc ggcgcgccct gcgggtgcac
      541 ggccttcagc gaacgctgga cttcgggcgg catatagttc tcatcgtgct tggcgaggac
      601 ctcgtcggcc gtgaatacgc cgtcggcgcc gaggcggcca tgcgcgatga ccccctgccc
      661 ttcacggaac aggtccggca gcacgcccgt gtagtgcacc ggcacggatt gctcgaagtc
      721 ggtcacgacg aactgcacct gcaggctgcc cgggctgcgc accacgctgc cctcctcgac
      781 catgccgccg agccggaagc gttcgcccgc cggcgcctcg cccttggcga tctgcgaagg
      841 atcgaagaag aacatcacgt tctcgcgaaa ggcctgcagc gtcagtacgc cggcgatcgc
      901 gactccggcc aggatgccga gcacgaggaa caggcggcgg cggcgcggcg tcacggggca
      961 ttctccatgg ccagccggcg tcgcgcctcg gcgcgggctt cgcggaacga acggcgggcc
     1021 gcgaacacgt tccacgcgat gatggcgcag gtcagcagga ccgacggcca caccgaggag
     1081 gcgtaaccac ccatggagag aaaatcgctc attgcgccac gacctcggcg acccatcgcg
     1141 actggcgctc gcggcgcagg atctccccgc gtacgcgcac cagcaccagg gccgcaaaga
     1201 acagtgtaaa cccgacgaac atgatcagca gcggcgacag catcggccag ctgatcgaag
     1261 gcttgccgaa cttcatgacc gatggtgcct ggtggatcga attccaccat tcgaccgagt
     1321 agtggacaat cggtacattc accaccccga cgatcgccag aaccgcgctc gcgcgatcgg
     1381 cgcgctgcgg atcctcgatc gccgctcgca ggcccatata ccctagatag agaaagagca
     1441 gcacgagttc cgcggtgagc cggggatccc aggcccagta tgtgccccac atgggcttgc
     1501 cccacaacat gccggtgacg agcgcggcga ccgtgaacca cgcaccgatg ggtgcgcagc
     1561 ttgcggcgac ggcatgggcc actttcatgc gccagatgag cccgagcgcc gcggccaccg
     1621 ccatcgttga atatgccatc agcgacagcc aggcgctggg cgcgtgcaca tagatgatgc
     1681 gaaagccgtc gccctgctgg taatcgggcg gcgcgagcac gaggccgcca tagaggccgg
     1741 cggcgagcag gatcgcggcc ggccacgcga accagggcac gagcgtgccg gccagcctgt
     1801 agaggtgggg cggcgagccg aagcggtgaa accaggtcca gtccatctct actccaggct
     1861 gatgcgcacc gccgcagccg cggcgagcgg cgcgagcgtg atgccgagta cggtaaacgc
     1921 agccagaaaa tacatcggtc ccgcgatccc tgttccggcg atcgcgcgcg ccgtcgcctg
     1981 cgcgccgaat atcagcagcg gtacggccag tggcagggtc agcaacgaca gcagcgcgcc
     2041 gccccgccgg atgccgatcg tcaggccggc tgtcaccgcg cccagcaggc tcagcgcgac
     2101 cgtgccgagc gccacggagg cgacgatgcc gggcaccgcg ccgaccggca caccgagtcc
     2161 gtaggccgcc accgggctca tcagggcgag cggcaggccc gcgagcagcc agtgggacac
     2221 tgtcttcgcg aacaacagcc aggtcaggga ctgcccggac aaggcgtact gctcgagcgt
     2281 gccgtcctgc gcatcgtcac gaaacaggaa ctcgatcgcg agcaacgagg atagcagcgc
     2341 ggccacccac agaacaccgg gcgcgacgtc gcgcaagcgc gacgcctcgg gcgacaaggc
     2401 cagcggaaac agcgtcgtca cgatcccgaa gaacagcagc ggatgcagaa gttgcgcgcg
     2461 attgcgaaaa gcgagacgca tgtctcgctc cacgaccaga cgtgctgcgg accaggggcc
     2521 tgtcatttcg tggacagtcc gagccggcgc gtgtgggcct cgtcgaccgg cagcgctgcg
     2581 tgcactgccg cgatgacgat gccgcccgtc ttgatccggc tttcgaccag gtgacccacg
     2641 agcgcgtgcc cggccgcatc gagatgggtc gccggttcat cgagcaccca cagtggcgcc
     2701 gccgcgagca gcacgcccgc gagcgccacg cggcggcgct ggcccgccga cagggtccgc
     2761 acgggccgct cggcgaaacg cgaggcgtcc acctcatcga gcgcgcggcc gatggcggcg
     2821 gcatccaccg ggcggcggat gccgatggcg taacgcaggt tttccacggc gctcaggtca
     2881 cccttgagcg gcgattcgtg cccgagccag gcgagctgcg cgtggaaggc gcgcgggtcg
     2941 gcatggatgc tgcgtccgtt ccattcgaca ctgcccgatt cggcgtgcac cagcccgacg
     3001 atggtacgca gcaggctggt cttgccggcg ccgttggcgc cactcacctc gaggtactcg
     3061 ccgctcgtgg cggcaaacga caggccgcgc aacacgtgcc gctcgccgcg aaagacatgc
     3121 agggtatcgc ccgtgattcg atgcgcggac attttttcat tctatcatcc cgccgccatc
     3181 gctctgacga ttacgattcg cgcagtgccg tggtcaccgg cagccgcgcg gcgcgcacgg
     3241 ccggcagcag cccgccgatc aggccgatcg ccagcgccca acgcacaccg gtgccgagta
     3301 gaggcggcga gacgtcgaag cggaagacga cctggctgaa attcgcgccg agcgtcgaga
     3361 ccgcgtagtc gtggaacagc agccacgcga tcaacgcgcc gagcgcgccg ccgagcagag
     3421 cgagcagcat ggcctcgagc acgaccgaca cgagcaccgg tgtcgcgccg aaaccgatcg
     3481 cacgcagcgt cgcgacctcg cgggcccgcg cattgaccgc cgcgtacatg gtattgagcg
     3541 cgccgaaaac cgcaccgatc gccatgatcg cggcgatcac gatgccgacg atgcggatca
     3601 ccttgctgag gcccgccgat tgccgcgagt agtagtcccg ggtcgtgcgc gcatcgacct
     3661 tcaggcgcgg atcgcttgcc agcgccgcct tgagggcgtc gaaggcagcg ggcgattcga
     3721 gccgcacggt gaccgactgg aagccagtac gccggtaggt cgccgcgacg gtctcggcat
     3781 cgccgatcag ctccgattcg tgcgcgtccc ccgatgcgaa cacaccgacg atctgccatt
     3841 gctgcgcgtt gaggatgatc gtctcgcccg gctcgaggcc ggcgaactgc tggcgggcgc
     3901 cctgcccgac cagcaactcg cgcagtccag gctcgaatcg gcgtccctcg acgatccgca
     3961 cgttcggccg cacggcccag gaccggggcc cgacgccgcg tatctccacg ttcgcgtcgg
     4021 tgccgctcga cttcttcggc acgttggtca cgacgacgac ctcggccgat acgatcggct
     4081 gtccgctggc atctcgcgct atgccgggag cctgcccgat caatgtggcg ctctcgcggc
     4141 tgagccccga atcgagctcg gcgctggcgc cgccgcgcac gacgattgcg gtgtcgtcgt
     4201 tgccggtgcc gcgcagggtc tgctgcaggc cgccggcgag cgcgagcagc gcgaccagta
     4261 cgcccacgac cccggcgatg ccgatgatga tcaccgagga ggcgccgagg cgctgcggca
     4321 gcgtcgcgat gccgatcctg gtgatcgccg cggcctggcg gccgacgcgt gtcaaaccca
     4381 tccatgccgc gagcaggata gcgagcccga gcaacgcggc ggcgggcagc acgatccaaa
     4441 gcgcaatcga cacgacgatc aggagcgcca tcagcaggcc gcgcagcaat ccggtgaagt
     4501 gttgcatcac ggttccctca gcgtccggca agcgcgtcga cgacgcgcag ccgcatcgcc
     4561 cgccacgccg gcaggatgcc gacgatcgcg ccgaccgcga tcatcagcgc gatgccgatg
     4621 ccccaggatc gccacccgac cggcggcatc tggatcatgc cgccgctcgc ctcggtgacg
     4681 atcggcatca gcagcgtcgc gagcacgagt ccgaacacgc cgccgatcag gatcagcagc
     4741 accgactcgc cgaacacgag cgcgagcacc tgccggtcgg tgaagccgat cgtcttcaac
     4801 gtcgcgagct cgggcatgcg ctcacgcacc gcctgcgcca tcgtgttgcc cgtcagcagc
     4861 atcagcgtga aaaacaccgc gccgatgatc gccgtgacga tcaggccgat gtcgccgatc
     4921 tgcttcgcga acgacaggtt gaattcctgc tcggtctggg tcttggtctc gtggtcggaa
     4981 ttggcggaca gcgcatcgat cgcctgtgcg accctggccg agtcggcggg gtcggcgacc
     5041 ttgacgacga tccagccgac ctggccgccg ccgtaggcgt tcgcttcgtc gaaatacttc
     5101 cagttgagca gtatctgctg ctcgagcgcg cggttcgccg cgagcggcgc ctggtagatg
     5161 ccgacgatgt cgaaggtcca ggtcgtctcg ccggtcgcct tgttcgggaa gatcgagccc
     5221 agaatcggca gcttgtcgcc caccttccag ccgaatcggt cggcgaccgc cttgccgacc
     5281 agtacgcccg agcgcgtcga ggcgagcgct tcggccatcg cgggggtcac gccgtagtcg
     5341 ggataggcat cgaaatatcc cgggccgatc gcgaagatga tcatctggtt cttctgctcg
     5401 cggaagtagc cgccgaacca gttggcccag gtaacaagcc gcacgccctg caccgccgcg
     5461 acgcggttga cgaggctctc gggcaggctc tggatgatcg agaagcgcga agatacgacc
     5521 agccggtcga caccggcgag cgtaccgccc gaattgaatg cgacccgcac gccgtcgagc
     5581 agcccgaaca gcaggaatgc cgcgagtacc gacaggatgg tgagcaccgt gcgggtcttg
     5641 cgccgcatca gggccgcccg gatcaggtgc aggtacttca cggcagcgac tcgaccagtg
     5701 aacctttctc gagatgcagc gtatggctcg cgtgatcggc ggccttggga tcgtgcgtga
     5761 ccatgatgat cgtcttgccc tgctcgcggt tcaggacctg cagcaggttc aggacttcct
     5821 cggcggactg gcggtcgagg tcgccggtcg gctcatcgca gacgagcagc gtgggatcgg
     5881 aaatgatggc ccgggcgatc gcgacgcgct gctgctggcc gcctgacagc tcacctggct
     5941 tgtgcgaggc ccgatcggcc aggttgacga gctgcagcgc gatcttcgcg ttctgccggc
     6001 gctgcgcggc cgacagtttc gtcagcagca gcgggacctc gacattcttc tgcgcggtga
     6061 gtatcggcag caggttgtag aactggaaga tgaagccgac gtgggccgca cgccatttcg
     6121 cgagcgccgt gccgccgagc tggtcgatgc gcgtgccgtc gacactgatg ctgccgctcg
     6181 agggcgagtc gaggccaccg atcaggttga ggagcgtcgt cttgcccgaa cccgaggggc
     6241 ccatcagcgc gaggaaatcc cccttgcgga tatcgagggt gatgtggtgc agcacctcga
     6301 cggtctggcg gccccgcacg tacgccttgc tcacatcacg gatctcgacc agtgcactca
     6361 cgtgccctcc tccctgatcc tcgtaccatc ctggatcgcc tcgcccggcg cgcgcacgac
     6421 gcgtgcaccc actgcgacgt cgtcgacctg cgtcaggtcg ccgaagcgct gcccgggccg
     6481 caccggctgc gcacgcgcgc ggtcgccgtc ggccaagaat acgacatccg caccgtcccg
     6541 ccggacgatc gcgctcgacg gcacgagcac gcctgcgcca gccccgcccg tcgcgccaac
     6601 cgcggtctcc ccggcgagca acgagacccg cgcgcccatg tcgggcagga tccgagagtc
     6661 cttctggtcg aggccgatgc gtaccttgac cgtcgccttg ccgcggtcgg ccgccgggat
     6721 gatcgcgatc acgtgcgccg ggatcgtcca gtcctggtaa gcgtcgagca ccgcctgcgc
     6781 cggtccgccg ggcaccaccc ggttcaggta cgcctcgttg acgtcgactt cgatctcgag
     6841 cgaattcatg tcgacgagcg tgccgatgcc cgtgcgcgtg aagccgccgc cggccgacag
     6901 cggcgaaacg atctccccga cctgggcggc cttggcgatc acgacgcccg cgaacggtgc
     6961 ccgcaccgtc gtgtagtcga gctgcacctg cgcgctgcgc acctgtgcct ctgcgaggtg
     7021 cacggtctcc tgctgcgcgg cgagctgcgc ggccagcgaa tcgacctgca ggcgcgcggc
     7081 ctcgacagcc tgctcggcca cgagctggcg cgcgagaagg tcctgctggc gctcgaggtc
     7141 gcgccgcgcc tgcgcgagtt cactcgtgta ctggccgacg cgcgcgcggg ccgcagccag
     7201 ctgcgcctgc gtctcggcga gttgcgcacg ctgcgagctg ttctcgaggc gcgcgagtac
     7261 ctgcccggcc tcgacactct cgccctcctc gatcagcact tcggtgagct tgccggtgat
     7321 ctgcgccgac acggttgcct ggcgacgcgc cgtgacatag cccgtcgcct gcagcacggc
     7381 gctcgccccc gagccggccg cagccggcgc ggccgccgtg gccagctcga cgtcgacgac
     7441 cgcagtcgcg cgactccacc accagaaggc ggcgcctgcg agcagcagca gcgcagtcac
     7501 gccgcctgcg atccaggtgg cgcgtggcgt ggtgctctcg tcggtccggt cgatgcgcag
     7561 ttgcgcgagc agcctgccct tgtcgttctc gttggtgtcg gccatgggc
//
LOCUS       DAOQVY010000117         7556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_10748_length_7556_cov_5.128783, whole genome shotgun sequence.
ACCESSION   DAOQVY010000117 DAOQVY010000000
VERSION     DAOQVY010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7556
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10748_length_7556_cov_5.128783"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..872
                     /locus_tag="PKN91_10280"
     CDS             282..872
                     /locus_tag="PKN91_10280"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HNS28496.1"
                     /translation="MSTPFAGWTAALATLALVGGCASAPQINVMSDPAANIAAYKTFN
                     YMSPLGTDRGGYESLVSANLKDSTTLELTRRGYQMSETPDFLVNFSGRLDEKLRVSTS
                     PSFSPGMGYYGYRTGFYDPWYGYTDVNVDQYTQGTLNIDIVDAASKRLVWESVAAGRV
                     TAKTREDLPGAIRKIVPEMLAKFPSVGGGPPPTTEK"
     gene            complement(903..1559)
                     /gene="rpiA"
                     /locus_tag="PKN91_10285"
     CDS             complement(903..1559)
                     /gene="rpiA"
                     /locus_tag="PKN91_10285"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNS28497.1"
                     /translation="MNAGNAGKQRAAHAALEYVEHGTVIGVGTGSTVNYFIEALARLR
                     GSIRGAVSSSLASTARLEALGIEVMDLNHTGPLDLYVDGADEATRDRYLIKGGGAALT
                     REKIVAAASRRFVCIVDESKLVPLLGRFPLPVEVIPMARSYVAREIVARGGQPVWREQ
                     CTTDNGNHILDVHGLEILDPPSLERDLNQIAGIVTVGLFAQRPADLLVIGNEKGVTVV
                     "
     gene            complement(1849..2313)
                     /locus_tag="PKN91_10290"
     CDS             complement(1849..2313)
                     /locus_tag="PKN91_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNS28498.1"
                     /translation="MNHWLMKTEPTTFGVDDLARAPKKTTMWDGVRNYQARNMLRDDF
                     AKGDRALLYHSSCAVPGVVGVMRVTRAGYPDPSAFDRNDSHYDAGSDAANPRWYAVDV
                     QLERRLARVITLEELRRHATRKLAGMILLQRGSRLSVMPLTKAHYEFILSLE"
     gene            complement(2310..2900)
                     /locus_tag="PKN91_10295"
     CDS             complement(2310..2900)
                     /locus_tag="PKN91_10295"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNS28499.1"
                     /translation="MTSALRKTLRARRHALGAGTRTRLSRAITRHLLAAIGPTVRGRH
                     IAGYAALADEPDLGAALAALAHRGALIYLPRIDNYRLGRMSFALCDGDLRPNRYGIGE
                     PGRAAPARRAAALDIALLPLVGFDAHGVRLGMGGGYYDRAFAFRRQPRAVPRPRLIGI
                     AFACQQVDALPSAPHDVRLDAVVTESGLTRFARRTG"
     gene            complement(2921..3106)
                     /gene="ssrS"
                     /locus_tag="PKN91_10300"
     ncRNA           complement(2921..3106)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKN91_10300"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3148..3465)
                     /locus_tag="PKN91_10305"
     CDS             complement(3148..3465)
                     /locus_tag="PKN91_10305"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNS28500.1"
                     /translation="MSEREVARLSVRILEKEYFVACPYEERSDLLDSAEYLNGKMREI
                     RDGGKVVGLDRIAVMAALNLANELLRLRNQGQRLDQDFGGRVRALRERVESALAEGQQ
                     LEL"
     gene            complement(3462..3692)
                     /locus_tag="PKN91_10310"
     CDS             complement(3462..3692)
                     /locus_tag="PKN91_10310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HNS28501.1"
                     /translation="MSENTRPPIDTELRRLEKRLDELVGIVGRLREENRALRQRQDTL
                     STERAALVQKNEQVRNRVEAMIGRLKTMEHGA"
     gene            3762..4322
                     /locus_tag="PKN91_10315"
     CDS             3762..4322
                     /locus_tag="PKN91_10315"
                     /inference="COORDINATES: protein motif:HMM:NF015642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HNS28502.1"
                     /translation="MTAMGYAELQRVLGDAQGLTEAAEAHGTLTGALCAAGAYRLEDW
                     LGEILPDGRADPMTRESCRACFEATARALRDADMAFQVLLPDDDQPIDARATALGQWC
                     QGFLYGLGTSSIDDVTALPGEVGEVVRDLSEITRIGVDARESIESNESAYAELVEFVR
                     VGVQLLFEELAPFRVSTAPPAAESLH"
     gene            4336..5661
                     /gene="pepP"
                     /locus_tag="PKN91_10320"
     CDS             4336..5661
                     /gene="pepP"
                     /locus_tag="PKN91_10320"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HNS28503.1"
                     /translation="MARLKTIPRAEFTRRRRQLMRLMGRDSIAIVPAAPVRLRNSDVE
                     YPYRQDSDFHYLTGFDEPESVAILVPGRAQAEYLLFVRDRDPARETWDGRRAGPAGAT
                     RDYDADDAFPISDIDEILPGLLENREKVYYAMGVHPDFDQRVVGWVNGLRAQAKHGRH
                     SPQEFVALDHVLHDMRLFKSRAELDMMRAAARIGAAAHVRAMRACAAGRHEYELAAEI
                     THEFRRHNADHSYQPIVGGGENACILHYRENDAALQAGDLVLIDAGCEYQSYASDITR
                     TFPVNGRFTAAQRAVYDIVLEANLAAIAKVRPGNHWNEPHDAAVRVITQGLVRLGLLK
                     GRVPTLEKALAYKRYFMHRTGHWLGMDVHDVGDYKLGDEWRVFEPGMVLTIEPGIYIP
                     HSARAAPKRLRGIGVRIEDDVVVTRSGCEVLTSGVPKQAADIEALMGAA"
     gene            5658..6917
                     /gene="ubiH"
                     /locus_tag="PKN91_10325"
                     /gene_synonym="visB"
     CDS             5658..6917
                     /gene="ubiH"
                     /locus_tag="PKN91_10325"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HNS28504.1"
                     /translation="MSAAATEVDVAIVGGGMVGASLALALRDTDLRVALIEGFAPDAA
                     AQPSFDERTTAIGNGSRAVFEALGAWPRMAGDGGVIRAIHVSDAGRYGFARLVAADLG
                     LEAFGCVLPNRVIGRALWEGLAQAPRLTTFMPARPVDARAGADRASLEVERADGSRAT
                     LHAALLVAADGAQSTLRAAFGLPSHVEDYGQVAVVANVAADRAHDGTAYERFTSVGPL
                     ALLPLADGSYTVVWARTPADAAEAREMPDAQFLAQLQQRFGWRAGRFVRVGRRVTYPL
                     ALTRSDATHATRLVLIGNAAQALHPVAGQGFNLGLRDAATLAEVLVDARAGGADIGGE
                     AVLARFAAARAADRRGVTGFTDGLVKLFRDDRPGVGALRDLGLLLFDLAPPAKRVLSR
                     VSWGFTGRLPRLARGLDVHGRSPTEPR"
     gene            6914..>7556
                     /locus_tag="PKN91_10330"
     CDS             6914..>7556
                     /locus_tag="PKN91_10330"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS28505.1"
                     /translation="MSIDCDIVVVGGGAVGAAFAALAVRQAQVPASRVLVIERERPPA
                     WERAAPPDLRVVALSRASSRILAAAGAWEAIVAGRVSPYERMRIWHESQSPLGGDALV
                     FDAAELGEPNLGHIVETRLIQSALLASLEAAGVEVLIGNVAALELSPRHAGVEVGGRR
                     ISARLVVGADGARSRVRELAGFVTRRRDFRQQAIVANVEVARPHERTAWQRFLG"
BASE COUNT         1127 a         2548 c         2668 g         1213 t
ORIGIN      
        1 cctagtcccc cagttagaag caggaggccc gtcgcaagac gggcctcttt gcgtttatag
       61 tagatatatg cgcgccctcg cgtcctgcgc ccccgtgccc gcactccgtt tcactgatga
      121 gtgcgtgacg ctcttccacg agacgggccg cgacgcgccc gattcgttga attccggcag
      181 ggtggccagg tgggaaggcc ccctgaacgg tgcgcacggc ccctggcggg tcgatcccga
      241 tgcgatcccg gccgccaaac actagacact gaggagtgat catgtcgaca ccatttgcag
      301 gatggactgc ggcccttgcg acgctcgcgc tcgttggcgg atgcgcatcg gccccgcaga
      361 tcaacgtgat gtcggacccg gcagcgaaca tcgccgcgta caagaccttc aactatatga
      421 gcccgctcgg cacggaccgc gggggctacg agtcgctggt ctccgcgaat ctgaaggact
      481 cgaccacgct cgagcttacc cgtcgcggct accagatgtc cgagacgccc gacttcctgg
      541 tgaatttctc gggccggctg gacgaaaagc tgcgcgtatc gaccagcccg tcgttctcgc
      601 ccgggatggg ctactacgga tatcgcactg gtttctatga cccgtggtac ggctacaccg
      661 acgtcaacgt cgaccagtac acgcaaggca cgctcaacat cgacatcgtg gatgcggcct
      721 cgaagcgcct ggtctgggaa tccgtcgccg ccggccgggt caccgcgaag acgagggaag
      781 acctgcccgg cgcgatccgc aagatcgtgc ccgagatgct ggcgaagttc ccgtcggtcg
      841 gcggtggacc gccgcccacc accgagaaat aagccgcgcg cgtgatcgaa gcggggtcac
      901 ggtcagacga ccgtgacccc tttttcgttg ccgatcacca gcaggtcggc cgggcgctgg
      961 gcgaagagac ccacggtgac gatacccgcg atctggttga ggtcgcgctc taggctgggc
     1021 ggatcgagga tctcgaggcc gtgcacgtcg aggatgtggt tgccgttgtc ggtggtgcac
     1081 tgctcacgcc agaccggttg gcccccgcgc gcgacgatct cccgggcgac gtaggaacgc
     1141 gccatcggaa tcacctcgac cggcagcggg aatcggccga gcagcggtac gagcttgctc
     1201 tcgtcgacga tgcatacgaa gcgcctcgat gcggccgcga cgatcttctc gcgggtcagg
     1261 gcggcaccgc cccccttgat caggtaccgg tcgcgcgtcg cctcgtcggc cccgtcgaca
     1321 tagaggtcga gcggtccggt gtggttcagg tccatcacct cgatgccgag ggcctcgagc
     1381 cgcgcggtcg aggcgagcga gctcgagacc gcgccgcgta tcgagccgcg gagcctggcg
     1441 agcgcctcga tgaagtaatt gacggtcgag ccggtgccca ccccgattac ggtcccgtgc
     1501 tccacgtatt cgagggctgc gtgtgccgcc ctttgttttc cggcatttcc tgcattcatc
     1561 gccgcctgct ccaataacgg taccccggaa acgactgtgc ccgctttcgc gggcaccagt
     1621 gtcgtcggac tttgaagccg gcgagcaagg aaggtctttc caagctcgcc gaaggtgtaa
     1681 aggccttact tcttcttcgc agctttcttc ttggccttct tcttggcttt cgccatgaga
     1741 gtctcctgtt gggttagtcc gaggtcgagt atatacacgc gtattcaaat tacaagcaag
     1801 cgtgcttgca tcgagtcgcg tgatctgcat cacacgtcgg gcaatcgatc actcgagcga
     1861 gaggatgaac tcgtagtgcg ccttggtgag cggcatcacc gagaggcgac tgccccgttg
     1921 cagcaggatc atgccggcga gcttgcgcgt cgcgtgccgg cgcagctcct cgagcgtgat
     1981 cacccgcgcc agtcgccgct cgagctgcac gtcgaccgca taccagcgcg ggttcgcggc
     2041 gtcgctgccg gcatcgtagt gggagtcgtt gcgatcgaag gcactggggt ccggatagcc
     2101 ggcgcgagtg acgcgcatca ccccgacgac gccgggcacc gcgcagctcg agtgatagag
     2161 gagggcccgg tcgcccttcg cgaagtcgtc gcgcagcatg ttgcgcgcct ggtagttgcg
     2221 caccccgtcc cacatcgtcg tcttcttcgg ggcgcgcgcg aggtcgtcga cgccgaaggt
     2281 ggtcggttcg gtcttcatca gccaatggtt catcctgtcc tccgtgcgaa gcgggtgagg
     2341 ccgctctcgg tgacgaccgc atcgaggcgc acgtcgtgcg gggcggaagg cagcgcatcg
     2401 acctgctggc aggcgaaggc aatgccgatc aggcgcgggc gcggcaccgc acgcggttgc
     2461 cggcggaacg cgaacgcgcg gtcatagtag ccgccgccca tgccgagtct caccccgtgc
     2521 gcgtcgaagc cgacgagcgg cagcagcgcg atgtcgaggg cggcggcgcg gcgtgcgggg
     2581 gccgcgcggc cgggctcgcc gatgccgtaa cgattcggcc gcaggtcgcc gtcgcagaga
     2641 gcgaaactca tccggccgag ccggtagttg tcgatgcgcg gcaggtagat gagcgcgccg
     2701 cggtgcgcga gggcggcgag tgccgcgccg aggtccggct cgtcagcgag tgcggcatag
     2761 cccgcgatgt gccggccgcg caccgtcggg ccgatggccg caaggagatg gcgcgtgatc
     2821 gcgcgcgaca ggcgggtgcg ggtcccggcg ccgagcgcgt gccgtcgggc gcgcagtgtc
     2881 ttgcggagcg cgctagtcat cgaggatggg aaggaaagca gcgccacccg cctgtgccgg
     2941 tgccggccgt tgctctcgac cggagcaaca agtggggcga ggtgcggcag gtaaggcttt
     3001 ccgcgcgagc ggacatgcac aattctgccg gcaatccaga tgccccatgg gtgtaccaat
     3061 tagggtcgag ggtaacccgg ccgactgtcg aacaccgcag gtggcgccat ggacactcta
     3121 cacgagtcgg cgccccggtg agcacgctca gagttcgagt tgctggcctt cggcgagcgc
     3181 cgattcgacg cgttcgcgca aggcgcgcac gcgaccaccg aagtcctggt cgagccgctg
     3241 gccctggttg cgcaagcgca gcaactcgtt cgcaagattg agcgcggcca tcaccgcgat
     3301 gcggtcgagc ccgaccactt tgccgccgtc gcggatctcg cgcatcttgc cgttgaggta
     3361 ttcggcggaa tcgagcaggt cggaacgctc ctcgtagggg caggcgacga agtattcctt
     3421 ctcgaggatc cgcacgctga ggcgtgcgac ttcgcgttcg ctcatgcacc gtgctccatg
     3481 gtcttcagcc gcccgatcat ggcctcgacc cggttcctga cctgctcatt cttctgcacc
     3541 agggcggcgc gctcggtcga gagcgtgtcc tgccgctggc gcaacgcgcg gttttcctcg
     3601 cgcaaccggc cgacgatgcc cacgagttcg tcgagccgct tctcgagccg gcgcagttcg
     3661 gtgtcgatgg ggggcctggt gttttcgctc atggaatgac tatgaaccgc gtggcgcggc
     3721 gggtcaatcg cgcaccggcg atcaggcata atgcacgtcc tatgacggcg atgggttatg
     3781 cggaattgca gcgcgtgctc ggggacgcgc agggcctcac cgaggcggcc gaggcacacg
     3841 gcacgctgac cggggcactg tgcgccgccg gcgcctaccg gctcgaggac tggctgggcg
     3901 agatcctgcc cgatggtcgc gccgacccga tgacgcgcga gagctgtcgc gcctgcttcg
     3961 aggcgaccgc ccgcgccctg cgcgatgccg acatggcctt ccaggtgctg ctgccggacg
     4021 acgaccagcc gatcgatgcg cgcgccaccg cgctcggcca gtggtgccag ggcttcctgt
     4081 acggcctcgg caccagctcc atcgacgacg tcaccgccct gccgggcgag gtcggcgagg
     4141 tggtccgcga cctttccgag atcacgcgta ttggcgtcga tgcgcgcgaa agcatcgaat
     4201 cgaacgagag cgcgtatgcc gagctggtcg agttcgtgcg ggtcggcgta cagctgctgt
     4261 tcgaggaact cgcgcccttt cgcgtgagca cagctccccc ggccgccgaa tcgctgcatt
     4321 gataccccgg gcgccatggc caggctgaaa acgatccccc gcgcggagtt cacacgccgc
     4381 cgccgccaac tgatgcgcct gatggggcgc gactcgatcg cgatcgtgcc ggcggccccg
     4441 gtgcgcctgc gcaacagcga tgtcgagtat ccctatcgcc aggacagcga tttccattac
     4501 ctcacggggt tcgacgagcc ggagtcggtc gcgatcctcg tgccgggccg ggcgcaggcc
     4561 gagtacctgc tgttcgtgcg cgatcgtgac ccggcgcgcg agacctggga cggccgacgc
     4621 gctggcccgg cgggcgcgac ccgcgactac gacgcggacg atgccttccc gatctccgac
     4681 atcgacgaaa tcctgccggg cctgctcgag aaccgcgaga aggtgtacta cgcgatgggc
     4741 gtgcaccccg atttcgacca gcgcgtcgtc ggctgggtca acggcctgcg cgcccaggcg
     4801 aagcacgggc ggcactcgcc ccaggagttc gtggcactcg accacgtgct gcacgacatg
     4861 cgtctcttca agagccgcgc cgagctcgac atgatgcgcg cggcggcacg catcggagct
     4921 gcggcgcacg tgcgggccat gcgcgcctgc gccgccgggc ggcacgagta cgaactcgcc
     4981 gccgagatca cccacgaatt ccgccgccac aacgccgatc actcctacca gccgatcgtc
     5041 ggcggcggcg agaacgcctg catcctgcac tatcgcgaga acgatgcagc gctgcaggcc
     5101 ggcgacctgg tgctgatcga cgcgggttgc gagtaccaga gctatgcctc ggacatcacc
     5161 cgcaccttcc cggtcaacgg tcgcttcacc gcggcgcagc gggcggtcta tgacatcgtg
     5221 ctggaggcga acctcgccgc catcgccaaa gtgcggccgg gcaatcactg gaacgagccg
     5281 cacgatgcgg ccgtgcgcgt catcacccag gggttggtgc ggctgggtct gctgaagggg
     5341 cgcgtgccga ccctcgaaaa ggcgctggcc tacaagcgct atttcatgca ccgcaccgga
     5401 cactggctcg gcatggacgt gcacgacgtc ggcgactaca agctcggcga cgaatggcgg
     5461 gtgttcgagc cgggcatggt gctgacgatc gagcccggca tctatatccc gcactcggcg
     5521 cgcgcggcgc cgaagcgcct gcgcggcatc ggcgtgcgca tcgaggacga tgtcgtcgtg
     5581 acgcggagcg gctgcgaagt gctgaccagc ggcgtgccga agcaggccgc ggacatcgag
     5641 gcactgatgg gggcggcatg agcgcggccg cgaccgaagt cgatgtcgcc atcgtcggtg
     5701 gcggtatggt cggcgcaagc ctggcgctcg cgctgcgcga caccgacctg cgggtcgccc
     5761 tgatcgaggg gttcgcgccg gacgccgcgg cgcagccgag cttcgatgag cgcacgaccg
     5821 cgatcggcaa tggcagtcgc gcggtgttcg aggcgctggg cgcgtggccg cgcatggcgg
     5881 gtgacggcgg cgtcattcgc gcgatccacg tgtccgacgc gggtcgctac ggatttgccc
     5941 ggctcgtggc cgccgacctc ggcctcgagg ccttcggctg cgtgctgccg aaccgcgtca
     6001 tcggacgcgc gctgtgggag gggctcgcgc aggcaccgcg actcacgact ttcatgcccg
     6061 cgcgcccggt ggatgcgcgc gcgggcgccg atcgcgcgtc gctcgaagtc gagcgcgcgg
     6121 acggctcgcg cgcgacactc cacgccgcgc tgctcgtggc cgccgatggt gcgcaatcga
     6181 cgctgcgcgc cgcgttcggc ctgccgtcgc atgtcgagga ttacggccag gtggcggtgg
     6241 ttgcgaacgt ggcggccgac agggcgcacg acggcaccgc ctacgagcgc ttcacgtcgg
     6301 tcgggccgct ggcgctgctg ccgctcgccg atggcagcta cacggtggtc tgggcgcgca
     6361 cgcctgcgga cgcggccgag gcgcgggaga tgcctgacgc gcagttcctc gcgcagctgc
     6421 agcagcgctt cggctggcgc gccggccgct tcgtgcgcgt cggcaggcgc gtgacctatc
     6481 cgctcgcgct cactcgcagc gacgccaccc atgccacgcg cctcgtgctg atcggcaacg
     6541 ccgcccaggc actgcatccg gtggcgggtc agggcttcaa cctcgggctg cgcgatgcgg
     6601 cgacgcttgc cgaagtactc gtcgacgcgc gcgccggcgg cgcggacatc ggcggggaag
     6661 ccgtgctcgc gcgcttcgcc gcggcgcgcg ccgccgaccg tcgcggcgtg accggattca
     6721 ccgacgggct cgtgaagctc tttcgcgacg atcgaccggg tgtcggcgcg ctgcgcgacc
     6781 ttgggctgct gctgttcgat ctcgcgccgc cggccaagcg cgtcctgtcg cgcgtcagct
     6841 ggggtttcac cgggcgcctg ccgcgcctcg cacgcggcct cgacgtgcac ggccgttccc
     6901 ccacggagcc gcgatgagca tcgactgcga catcgtcgtc gtcggcggcg gagcggtggg
     6961 cgcggcattc gcggcgttgg cggtgcggca ggcgcaagtg ccggcgtcgc gcgtcctcgt
     7021 gatcgagcgc gaacgtccac ccgcctggga gcgggcggcg ccgcccgacc tgcgcgtggt
     7081 cgcgctgtcg cgcgcgagct cgcgcatcct cgcggccgcg ggggcgtggg aggcgatcgt
     7141 cgcggggcgc gtgagcccct acgagcggat gcgcatctgg cacgagtcgc agtcgccgct
     7201 gggcggggat gcgctggtat tcgatgcggc ggaactcggt gagccgaatc tcggccacat
     7261 cgtcgagacc cggttgatcc agtcggcgct gctcgcctcg ctcgaggcgg cgggcgtcga
     7321 ggtgctcatc ggcaatgtcg cggcgctcga gctgtcgccg cggcacgccg gagtcgaggt
     7381 gggcgggcgg cgcatcagcg cgcggctggt cgtcggcgcc gatggagcgc gctcgcgcgt
     7441 gcgcgagctt gcgggcttcg ttacgcgcag gcgcgatttc cgccagcagg ccatcgtcgc
     7501 caatgtcgag gtggcgcgcc cgcacgagcg cacggcctgg cagcgtttcc tcgggc
//
LOCUS       DAOQVY010000118         7534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_10782_length_7534_cov_5.890226, whole genome shotgun sequence.
ACCESSION   DAOQVY010000118 DAOQVY010000000
VERSION     DAOQVY010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10782_length_7534_cov_5.890226"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /gene="ppnN"
                     /locus_tag="PKN91_10335"
     CDS             complement(<1..888)
                     /gene="ppnN"
                     /locus_tag="PKN91_10335"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HNS28506.1"
                     /translation="METRIVVPDLVDARVGPRGSLETLSQEEVDQLLDSSQAGLYPLF
                     RQCVLAVLNSGSETDNAREIFERYRDFDIEIVRHPWGIKLEIRNAPAMSFVDGEMIRG
                     IKEHVFAVLRDVVFISNEINDSTRFDLTRPESITNAVFHILRNAHLLDHKVPPNLVVC
                     WGGHSISREEYDYTKKVGYELGLRELDICTGCGPGAMKGPMKGGNIGHAKQRVRGGRY
                     IGITEPGIIAAEPPNPIVNQLVIMPDIEKRLEAFVRLGHGVMVFPGGAGTAEEILYLL
                     GILLDPENAQQPLPVILTGP"
     gene            complement(909..1469)
                     /locus_tag="PKN91_10340"
     CDS             complement(909..1469)
                     /locus_tag="PKN91_10340"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNS28507.1"
                     /translation="MSSKCLRALPFLAALVGGLLAGPAAADALVPAQRVSIVQPVAES
                     LPLADRVVVRKGERSLQLLRNGNVLREYKIALGLNPTGHKERSGDFRTPEGSYQLTRR
                     NPRSDYFLSIEVSYPNATDVRNARRNGWQAGGAIMIHGLPNELKRTPEYYERTDWTDG
                     CIAVTNSEMLEIWLLTPDNVPIDILP"
     gene            1579..2112
                     /locus_tag="PKN91_10345"
     CDS             1579..2112
                     /locus_tag="PKN91_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28508.1"
                     /translation="MGILHAALLLAAGLLAACATPAIDPVDEQLDRDTGTTITRLGRP
                     VELLATAPRGRNADPFAYLAPFETNRVGERRLFLWASVPGDSAAMPRLHCGELAASLE
                     APLAGGMPTVGMGLTRLPYRALAAWSAVHVIPIDAIWLDCLASDARLEVEVGEDRFAS
                     DGARSGDLAAFRERVRP"
     gene            complement(2172..4397)
                     /locus_tag="PKN91_10350"
     CDS             complement(2172..4397)
                     /locus_tag="PKN91_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNS28509.1"
                     /translation="MASMKRLVALLLCIATGVVHAQSAADVIPRPPELQADVDFWIRV
                     YSQITTNEGFLHDERDLSVVYETLRFAPNTQPKQRSEIVDEARKRYQAVFDRLLTDPP
                     VAELTGEEKRVRELWSKAGHERLKSAREGIRFQLGQADRFRAGLVRAGTWEAHIAQTL
                     ANLGLPPEIAALPHVESSFNPAVYSKVGAAGLWQFMRSTGRRYMRIDSAVDERMDPFR
                     STEAAAQLLAYNYRLLGTWPLALTAYNHGAAGMRRAAKATGTTDFVRINRSYRSQSFG
                     FASRNFYPSFLAALTIDQNPEKYFGPIERASELEFTEVAMPSYAPVKALERSLSIDRA
                     TLRELNPALLPTVWNGERLVPRGYRLRLPGNGRSLSSEELARLLPDTEQYVAQIESRS
                     HRVKKGETLASVAQRYGISTQRLASLNGLRVDAGVRQGRRLRLPERMPALVSARAAAA
                     AVASTAEPGRVYVVKRGDSLSAIAARSGVSEREVMRINGLRNPNYIFEGQRLRLSVDD
                     DEAPAAVVAAAEPAATPAAVEPASPEPVDAPAAAVADTAAVRESREDEAAAIASARRA
                     SRAEPVSAREAEALGPALVTGGESVPSSVDAVDYSVASDGSIRVVAAETLGHYADWLG
                     VSASRLRQINKLRGGQPVLLGNRLKLDLGKTTAAEFEQKRRDYHYALQANFFNVHRIL
                     GTEVYVVRRGDSMWRITQRYARVPAWLLQQYNPDLNLGELRPGAQIVVPKVEAVAEGS
                     G"
     gene            4631..5386
                     /locus_tag="PKN91_10355"
     CDS             4631..5386
                     /locus_tag="PKN91_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4114 domain-containing protein"
                     /protein_id="HNS28510.1"
                     /translation="MVRFSLPLACASLALAAAAHAAPINITGANGEHNLQQILDGITV
                     GGPSSIDVVNDQYAHDSLWSLGGSGGSFSQIVIELAGYANVNSFGIYDALDPTRRVEL
                     FSGSASAGSSSGRAVFSILDDGHVVRNLFDETGVYFAANVFGFYLASVGNTWFSDAAL
                     NSDGADHMLAYQGAGDTVKIGNTAPGLWGSNEFVMAWEDLPLGSWDYDYNDFVVMVES
                     VNGVVSVPEPGTLALLGLGLVGLGFARRRHSAG"
     gene            complement(5562..6269)
                     /locus_tag="PKN91_10360"
     CDS             complement(5562..6269)
                     /locus_tag="PKN91_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28511.1"
                     /translation="MNAMKLDKKIIKYRVKKDEEKASEEKAPEAKAAAGSAKVIRMHE
                     EIQRPEVLIGSTYKIKTPVSDHAMYVTINDIVLNEGTEFEQRRPFEVFINSKNLDHYQ
                     WIVALTRIISAVFRKGGDVTFLVDELKAVFDPRGGYWQPGGKFMPSIIAELGHVIEKH
                     LQTIGLLRVPELDVHQQQFIAEKRAEYEERQKQADAFSKSHFPDGAQLCSKCSTAALV
                     MMDGCMTCLNCGDSKCG"
     gene            complement(6272..>7534)
                     /locus_tag="PKN91_10365"
     CDS             complement(6272..>7534)
                     /locus_tag="PKN91_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HNS28512.1"
                     /translation="EPGFILIDRVNEMNNNWWCENIRATNPCGEQPLPPYGSCLLGSV
                     NLTQFVRNPFTEFAEFDWNEYREVVKVFTRMLDNVVEVNGLPLEQQRNEIKRKRRHGM
                     GFLGLGSTITLLGMKYGSPESVSFTEDVAREMAVAGWEAGLDLAREKGPAPIMNEEFE
                     VTRAMLRKRPEMVEDGWKVGAHIPGRILHAKYSRYMQRVAEVAPRLVEQLAEVGARFT
                     HHSSIAPTGTISLSLANNASNGIEPSFAHHYFRNVIRPGRKTKEKVDVFSFELLAYRE
                     LVNPRAMPGAETEAERLPAYFTTAEDISPREHVDIQAAAQKWIDSSISKTANVPTDYK
                     YADFKDIYLYAHEKDLKGCTTFRFNPEAFQGVLVKEEDLRNTTYVFTLEDGSEIEVRG
                     DEEIEYDGEKHTAANLFDALKEGYYGKF"
BASE COUNT         1158 a         2516 c         2518 g         1342 t
ORIGIN      
        1 cgggccggtc aggatcaccg gcagcggctg ctgcgcgttc tccggatcga gcaggatgcc
       61 gagcaggtac aggatttcct cggccgtgcc ggcgccaccg ggaaagacca tgacgccgtg
      121 gcccaggcgc acgaacgcct cgaggcgctt ttcgatgtcc ggcatgatca cgagctggtt
      181 gacgatcggg ttcggcggct cggcggcgat gatgccgggc tcggtgatgc cgatgtagcg
      241 tccgccgcgc acgcgctgct tggcatgtcc gatattgccg cccttcatcg gccccttcat
      301 cgcgccggga ccgcagccgg tgcagatatc gagctcgcgc agccctagct cgtagccgac
      361 cttcttcgtg tagtcgtact cctcgcggct gatcgagtgc ccgccccagc agaccaccag
      421 gttcggcggc accttgtgat ccagcaggtg ggcgttgcgc aggatgtgga ataccgcgtt
      481 ggtgatcgac tccggccgag tcagatcgaa acgcgtgctg tcgttgattt cgttcgagat
      541 gaacaccacg tcgcgcagca ccgcgaacac gtgctccttg atgccgcgga tcatctcgcc
      601 gtcgacgaag gacatcgccg gggcattcct gatctcgagc ttgatgcccc aggggtggcg
      661 cacgatctcg atgtcgaagt cgcggtagcg ctcgaagatc tcgcgcgcgt tgtcggtctc
      721 gctgccggaa ttgagcaccg ccagcacgca ctggcgaaac agcggataga ggccggcctg
      781 gctcgaatcg agcagctggt cgacctcttc ctgggaaagt gtctcgaggc tgccgcgcgg
      841 acccacgcgg gcatcgacga gatcgggtac gacgatcctg gtttccatcc ctgccacgct
      901 agcgcgtgtc agggcaggat gtcgatcggt acattgtcag gcgtgagcag ccagatctcg
      961 agcatctcgg agttggtcac cgcgatgcag ccgtcggtcc agtcggtgcg ctcgtagtat
     1021 tcgggcgtgc gcttcagctc gttcggcagg ccgtggatca tgatcgcgcc gcccgcctgc
     1081 cagccgttac ggcgcgcatt gcgcacgtcg gtcgcgttcg ggtacgagac ttcgatcgac
     1141 aggaagtagt cgctgcgcgg attgcggcgc gtgagctggt agctgccctc gggagtacgg
     1201 aagtcgcccg agcgctcctt gtggccggtg gggttcaggc cgagcgcgat cttgtactcg
     1261 cgcagcacgt tgccattgcg cagcagctgc aggctgcgct cgcctttcct gacgaccacg
     1321 cggtcggcga gcggcaggct ctccgccacc ggctgcacga tcgacacgcg ctgcgccgga
     1381 accagcgcgt cggcggcggc tggcccggcc agcagccccc ccacgagggc ggccagaaac
     1441 ggtagcgcgc gcaggcactt ggaactcatg gcgcagatta taggtgcgct cgccggcgga
     1501 cgcgaccgtg cggctgtcac caggcggcga cgaggcgccg ggattgcggc aggacgtgag
     1561 ccacgtcacg atcggcgcat ggggatcctg catgccgcat tgctgctggc cgccggcctg
     1621 ctcgccgcct gcgcgacccc cgcgatcgat ccggttgacg aacagctcga tcgcgacacc
     1681 ggcaccacca ttacccgcct cgggcgtccg gtcgaactgc tcgccacggc gccgcgcgga
     1741 cgcaatgccg atccgttcgc gtatcttgcg ccgttcgaga ccaatcgcgt gggcgagcgg
     1801 cggctgttcc tgtgggcgtc cgtgccgggc gacagtgcgg cgatgccgag gctgcattgc
     1861 ggtgagctgg ccgcgtcgct cgaggcaccg ctcgcgggcg gcatgccgac ggtcggcatg
     1921 ggattgactc gcctgcccta tcgcgcgctg gctgcctgga gcgccgtgca cgtgataccg
     1981 atcgacgcga tctggctcga ctgcctggcg agcgatgcgc gcctcgaggt cgaggtcggc
     2041 gaggaccggt tcgcgagtga cggcgcgcgg agcggcgatc tcgcggcgtt ccgggagcgg
     2101 gtgcggccgt agcggcgagg agcggcccgg ccaccggacc gggggcgcag cagcggcgcg
     2161 cgccgcgcgc ctcagccgct cccctccgcc accgcctcga ccttcggcac gacgatctgc
     2221 gcacccggcc gcagttcgcc aaggttcaga tccgggttgt actgctgcag cagccacgcc
     2281 ggcacacgcg cgtaacgctg cgtgatgcgc cacatcgagt cgccgcggcg cacgacgtag
     2341 acctcggtgc cgaggatgcg gtgcacgtta aagaagttgg cctgcagcgc gtaatggtag
     2401 tcgcgccgct tctgctcgaa ctccgcggca gtcgtcttgc cgagatccag tttcagtctg
     2461 ttgccgagca ggacgggctg gccgccgcgc agcttgttga tctggcgcag gcgcgaggcg
     2521 ctgacgccga gccagtcggc gtagtggccg agtgtctcgg ccgcgacgac gcggatcgag
     2581 ccgtcgctcg ccacgctgta gtcgactgcg tcgaccgacg agggcacgct ttccccaccc
     2641 gtgaccagcg cggggcccag cgcctcggct tcgcgggccg acaccggctc tgcgcggctc
     2701 gcgcgccgcg cgctcgcgat cgccgccgct tcgtcctccc tgctctcgcg caccgctgcg
     2761 gtatcggcga cggccgccgc cggcgcgtcc accggctccg gcgacgcggg ctcgacggcg
     2821 gcgggcgttg ccgccggctc ggccgccgcg accaccgcgg caggtgcctc gtcgtcgtcc
     2881 acggagaggc gcaggcgctg gccctcgaaa atgtagttcg gattgcgcag cccgttgatg
     2941 cgcatcacct cgcgttcgct gactccgctg cgcgcggcga tggcggagag cgaatcacca
     3001 cgcttgacca cgtacacacg ccccggctcc gcggtgctcg cgacggcagc cgcggccgcg
     3061 cgggccgaca ccagtgcggg catgcgctcc gggagtcgca gcctacggcc ctgtctgact
     3121 cccgcatcga cccgcagacc gttcagcgag gcgagtcgct gcgtcgagat gccgtagcgc
     3181 tgcgcgacac tcgcgagtgt ctcgcccttt ttcacgcggt gggagcgcga ctcgatctgc
     3241 gcgacgtact gctcggtatc cggcagcagc cgggcgagct cttcgctcga gagcgacctg
     3301 ccgttgcccg gcaggcgcag acgatagccg cgcggcacga ggcgctcgcc attccacacc
     3361 gtgggcagga gcgcggggtt cagctcgcgc agcgtcgcgc gatcgatcga cagggagcgc
     3421 tcgagcgcct tgaccggcgc ataggacggc atcgcgacct cggtgaactc cagttcggac
     3481 gcgcgctcga tcggaccgaa gtatttctcc ggattctggt cgatggtgag cgccgccagg
     3541 aacgacggat agaaattgcg cgacgcgaag ccgaaggact ggctgcggta gctgcgattg
     3601 atgcgcacga aatcggtcgt gccggtggcc ttggccgcgc gacgcatgcc cgccgcgccg
     3661 tggttgtagg ccgtgagggc cagcggccag gtgccgagca ggcggtagtt gtaggcgagc
     3721 agctgcgcgg cggcctcggt ggaacggaac ggatccatgc gttcatcgac cgcgctgtcg
     3781 atacgcatgt agcgccggcc cgtcgagcgc atgaactgcc acagccctgc cgcgcccacc
     3841 tttgagtaca cggccggatt gaacgaggac tcgacgtgcg gcagcgccgc gatctccggc
     3901 ggcagaccga gattggcgag cgtctgcgcg atgtgcgcct cccaggtgcc ggcgcgcacc
     3961 aggccggcgc ggaagcgatc ggcctgcccg agctggaagc ggatgccctc gcgcgcgctc
     4021 ttcaggcgct cgtggcccgc cttgctccac agctcgcgca cgcgtttctc ttcgcccgtc
     4081 agctcagcga ccggcggatc ggtcagcagc cggtcgaaca cggcctggta acgcttgcgc
     4141 gcctcgtcga cgatctccga acgctgcttc ggctgggtat tcggcgcaaa gcgcagcgtc
     4201 tcgtacacga ccgacaggtc gcgctcatcg tgcaggaagc cttcgttggt ggtgatctgc
     4261 gaatagacgc ggatccagaa gtcgacgtcg gcctgcagtt cgggcgggcg cggaatcacg
     4321 tcggccgcgg actgcgcgtg cacgacgccc gtcgcgatac acagcagcag cgccaccagt
     4381 ctcttcatcg atgccatgcc cgcctcatct gtcggaataa ttgacagtcg aaccggcgcc
     4441 tcgcgcgaca tgtgcgcgtt gcgcagcgcg ctgcggccac gaaatgcggt accggtcaca
     4501 tcttcgcacc ggttcgcgca ccggctgagg acataatccc gaatttgtga agcatggcac
     4561 aagccttgct cctctccggg tgaagcgcgg ccgatttggc cgcccgcaac gcagagaggc
     4621 cctctcgata atggtcagat tctccctacc gcttgcctgt gcgtcgcttg cactcgccgc
     4681 agcagcccat gccgcaccga tcaacatcac cggtgccaat ggcgagcaca acctgcagca
     4741 gatcctcgac ggcatcaccg tcggcggtcc gagcagcatc gatgtcgtga acgaccagta
     4801 cgcccacgac agcctgtgga gcctcggcgg ctcgggcggt tcgttctcgc agatcgtgat
     4861 cgagctggcc ggctacgcca acgtcaacag cttcggcatc tacgacgccc tcgatccgac
     4921 gcggcgcgtc gaactcttct ccggctccgc ctcggcgggc tcgagctcgg gccgcgcggt
     4981 gttctcgatc ctcgatgacg gccacgtggt gcgcaatctg ttcgacgaga ccggcgtgta
     5041 cttcgccgcc aacgtgttcg gcttctacct cgcatcggtc ggcaacacct ggttctcgga
     5101 tgccgcgctc aacagcgatg gcgccgatca catgctcgcg taccagggcg ccggcgacac
     5161 ggtcaagatc ggcaatacgg cgcccggcct ctggggcagc aacgagttcg tgatggcctg
     5221 ggaagacctg ccgctcggct cctgggacta cgactacaac gatttcgtgg tgatggttga
     5281 atcggtgaac ggcgtggtca gcgtaccgga acccggcacg ctcgcgctgc tcggcctcgg
     5341 cctagtgggc ctcggctttg cgcgccgccg tcatagcgca ggataacgac aagaagaagc
     5401 actcctcagc aagcaagcaa gcgaccatgg ccccgttgtg agcggggccg tttttttggc
     5461 cgcgcctgcg cggcgagcgg agggagccac ctggacgcgg ttgcgtccgg tgacacatcg
     5521 ccgatggtgg gacgcgcgca gcgtggcggg ggggcagccc ctcaaccgca cttcgaatcg
     5581 ccgcagttca ggcaggtcat gcagccgtcc atcatgacca gcgccgcggt gctgcacttc
     5641 gaacacagct gcgcgccatc cgggaagtgc gatttggaga aggcgtcggc ctgcttctgg
     5701 cgctcctcgt attcggcgcg cttctcggcg atgaactgct gctggtgcac gtcgagttcc
     5761 ggcacgcgca gcaggccgat cgtctgcagg tgcttctcga tcacgtggcc gagctcggcg
     5821 atgatcgacg gcatgaactt gccgcccggc tgccagtagc cgccgcgcgg gtcgaacacc
     5881 gccttcagct catcgaccag gaaggtgacg tcaccaccct tcctgaacac cgccgaaatg
     5941 atgcgggtga gcgccacgat ccactgatag tggtcgaggt tcttcgagtt gatgaacacc
     6001 tcgaacggac ggcgctgctc gaactcggtg ccctcgttca gcacgatgtc gttgatcgtg
     6061 acgtacatcg catggtcgga caccggcgtc ttgatcttgt aggtcgagcc gatcagcacc
     6121 tcggggcgct ggatctcctc gtgcatgcgg atgaccttcg ccgatccggc ggcggccttc
     6181 gcctcgggag ccttctcctc ggaagccttc tcctcgtcct tcttcacgcg gtacttgatg
     6241 atcttcttgt ccagcttcat ggcattcatg ctcagaattt tccgtaatag ccttccttga
     6301 gggcatcgaa cagattggcg gcggtgtgtt tctcgccgtc gtattcgatt tcctcatcgc
     6361 cccgcacctc gatttcgctg ccgtcctcga gcgtgaacac gtaggtcgtg ttcctgagat
     6421 cttcctcctt cacgagcacg ccctggaacg cctccgggtt gaagcggaag gtagtgcagc
     6481 ccttgaggtc cttctcgtgg gcgtacaggt agatgtcctt gaagtcggcg tacttgtagt
     6541 cggtcggcac gttcgcggtc ttcgagatcg acgagtcgat ccacttctgc gcggcggcct
     6601 ggatatcaac gtgttcgcgc ggcgagatgt cctcggcggt ggtgaagtac gcgggcagcc
     6661 tctccgcctc ggtctccgcg cccggcatcg cgcgcggatt gacgagttcg cgataggcga
     6721 gcagctcgaa cgaaaacacg tcgaccttct ccttggtctt gcggcccggg cggatcacgt
     6781 tcctgaaata gtgatgcgcg aaggacggct cgattccgtt cgaggcattg ttcgcgagcg
     6841 acagcgaaat cgtgccggtc ggcgcgatcg agctgtgatg ggtgaagcgc gcgcccacct
     6901 cggcgagctg ctcgacgagt cgcggcgcca cttcggcgac acgctgcatg tagcggctgt
     6961 acttggcgtg caggatgcgg cccgggatgt gtgcgcccac tttccagccg tcctcgacca
     7021 tttcgggacg cttgcgcagc attgccctgg tgacctcgaa ctcctcgttc atgatcggcg
     7081 cggggccctt ctcgcgtgcc agatcgagcc ccgcttccca gccggccacc gccatctcgc
     7141 gcgccacgtc ctcggtgaag ctgaccgatt cgggcgaacc gtacttcatg ccgagcagcg
     7201 tgatggtgct gccgaggccg aggaagccca tgccgtggcg gcgcttgcgc ttgatctcgt
     7261 tgcgctgctg ctcgagcggc aggccgttca cctcgaccac gttgtcgagc atgcgcgtga
     7321 acaccttgac gacctcgcgg tactcgttcc agtcgaactc cgcaaactcg gtgaacggat
     7381 tgcggacgaa ctgcgtgagg ttcaccgagc cgagcaggca cgaaccgtag ggcggcagcg
     7441 gctgctcgcc gcaggggttg gttgcgcgga tgttctcgca ccaccagttg ttgttcatct
     7501 cgttgacgcg gtcgatcagg atgaagcccg gctc
//
LOCUS       DAOQVY010000119         7491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_10845_length_7491_cov_5.778375, whole genome shotgun sequence.
ACCESSION   DAOQVY010000119 DAOQVY010000000
VERSION     DAOQVY010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10845_length_7491_cov_5.778375"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PKN91_10370"
     CDS             complement(<1..213)
                     /locus_tag="PKN91_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNS28513.1"
                     /translation="MAMKNCRSPRCTCSATTLPDFLAFSAHKAYGPTGVGFLVAAPEL
                     LEELEPVQGGGEMIREVHLDRATWNEV"
     gene            320..2917
                     /gene="leuS"
                     /locus_tag="PKN91_10375"
     CDS             320..2917
                     /gene="leuS"
                     /locus_tag="PKN91_10375"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNS28514.1"
                     /translation="MQERYEPAAVEGSAQAAWQAANAFAADESAPGEKYYCLSMFPYP
                     SGRLHMGHVRNYTIGDVLARFMYMRGRNVLQPMGWDAFGLPAENAAMANGVPPAKWTR
                     ENIAYMKRQLQSLGFAIDWEREVATCDPAYYRWNQWLFLRMLEKGIAYKKTGVVNWDP
                     VDQTVLANEQVIDGRGWRTGAPIEKREIPMYYLNITAYADELLGALDGLDGWPDRVRT
                     MQANWIGKSVGCEIAFPYADDTSALMGSAGALKVFTTRADTLFGATFMAIAAEHPIAL
                     AAAGRDAKLAAFVDECRRGSTMEADVATQEKKGLPTGLHVCHPFTGERVGIWIANYVL
                     MGYGEGAVMGVPAHDERDFEFAGRHGIPVVTVVRSTRGAYDEVVAPWIDAYGEPGITV
                     ASGEFTGLESDAAVDAIAAKLEERGLGAKRVQYRLRDWGISRQRYWGCPIPLIHCPAC
                     GDVPVPDADLPVVLPEHLVPDGSGNPLARTPEFVNCSCPKCGGPARRETDTMDTFVDS
                     SWYFLRFASAGNDQAMVDARAEYWLPVDQYIGGIEHAILHLLYSRFWTRVMRDLGLVR
                     IDEPFARLLTQGMVLNHIYYRQEAGARRSYFNPADVEMRGGQAVLLADGQPVVHDGLG
                     TMSKSKGNGVDPQQLVEEFGADTARLFMMFTAPPEQSLEWSDEGVQGAFRFIRRLWKA
                     VHDLVSQGPLPDADFAVLTAPQRALRRLTHQTLAKVTDDIGRRHTFNTAIAAAMELLN
                     GATRIDTSEAAGRAVAREALEVIVLSLSPIIPHVTHVLWHALGHERALIEEPWPQPDP
                     AALARDEVELVVQVNGRLRGRITVAAEASDEVVRAAALAEENARKFINDAPVHKVIVV
                     PGKLVNIVV"
     gene            2921..3412
                     /gene="lptE"
                     /locus_tag="PKN91_10380"
     CDS             2921..3412
                     /gene="lptE"
                     /locus_tag="PKN91_10380"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNS28515.1"
                     /translation="MRRAIAVVSVALAAAALSTACGLRLQGREATPQAFASAHVEAPD
                     QQSDFVQALTRSLMASGVRVERDAARAEAVIRISRDQVREHVLSVSARNIPREYELTY
                     GVEFSVRRGEEELLAPQVAELTRDFTFDETILLAKEREQRMLEEALARDLAAIVMRQL
                     AAL"
     gene            3417..3944
                     /locus_tag="PKN91_10385"
     CDS             3417..3944
                     /locus_tag="PKN91_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28516.1"
                     /translation="MPVSAAVLRVNGTDRVALGLLLARFGMDLRLVAHGEVIPGSYWG
                     ESEAGLIGDRLYARLDTPLHSVLHEAAHYVCMTPERRTGLDRDAGGDDAEECAVCYLQ
                     VLLADALPGVGRERLCDDMDAWGYSFREGPTRAWLAGDARDAREWLLARDLIDRDDRP
                     TWRLRFSDPRSGPSP"
     gene            complement(3913..4998)
                     /gene="pip"
                     /locus_tag="PKN91_10390"
     CDS             complement(3913..4998)
                     /gene="pip"
                     /locus_tag="PKN91_10390"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HNS28517.1"
                     /translation="MAKRKKTAAKRKPALARRPAAPKKRATDKRAAGTRGGPKPAPGA
                     LRELYPPIEAYRTGRLAVSPTHTLYFEESGNPRGKPVVFLHGGPGGGTDPKMRRFFNP
                     KRYRIVLFDQRGCGKSRPRANLEDNTTWHLVNDIERLREHLGIERWQVFGGSWGSTLA
                     LAYAQAHPGQVTELVLRGIFLLRPWELEWFYGHPDGAGALFPELYAEFAAAIPPAERS
                     EMMDAYYRRLTSDDAQVRASAARSWSIWEGATSHLLFDPGEVKKFEEDAYAETFARIE
                     CHYFVNRGFLRSPTQLLDDVPKIRHIAAVIVHGRYDVVCPMKSAWDLHRAWPEADFRV
                     VPDAGHSAFEPGNIHELVRATDRFADR"
     gene            complement(5009..5359)
                     /locus_tag="PKN91_10395"
     CDS             complement(5009..5359)
                     /locus_tag="PKN91_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005695575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNS28518.1"
                     /translation="MPTLFERIIAGELPATILHQDERVTAFRDIHPRAPVHILIVPSK
                     PIPTANDIADEDETLIGHMFTVARDLARREGIAEDGYRLIINCNRHGGQEVYHLHLHL
                     LGGAPLGSMVSPGS"
     gene            complement(5437..>7491)
                     /locus_tag="PKN91_10400"
     CDS             complement(5437..>7491)
                     /locus_tag="PKN91_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS28519.1"
                     /translation="ALMLFLSRIRGLHCAAVLLIPVIPATLAAPPALEEVVVTASALR
                     QGVLETAQPVIVLGGDALHRELGQSLGETLAKQAGVTGTWFGPVASRPVIRGLGGERV
                     SMLEDGLGTQDVSALSEDHAVSIDPLVARSIEVIKGPGTLLHGNAAVGGVVNVVTSRF
                     AEKPLAQALQGAVELRGNSAASETSGAGRLDVRAGDFVLHADGYRRTSDDIDIPGNAL
                     SQRLAEQLAAQGETDPVNPHGTLYGSDGDSDGGALGVSYLASRGFLGVAASRTDANYG
                     TPGPESGADGSSVRIDMTQERYDLSGGLDDPFTGIDSMRLRAAHSEYFHQEFEPDGEA
                     GTTFAQDSGEARLSFDHAVLGGWRGTFGMQYRELDFRALGEEAFVPGNSMRNVGLFVF
                     EERPVGPLTVELALRYEDQRIEAEREAGLPDYSGDGVSGSAGLRWKFAPEYSVALNFT
                     RATRHPGATELYADGVHVALGRYERGDSAIGEEAALTTDLTLRKYAGAVQFTLGAFYS
                     RYDGYIHARPTGEFFEDPEEGELFPVVDYVQEDSSFHGLEAEVTLPLVARDAAALTLR
                     LATDYVRGKPDRGGNLPLMPPWRFGAELGFERGVWQAGLSIFRYADQDDVATNELPTN
                     GYTMIDADVSWHQPVGGGEIVAFLKGSNLADEEARRASSPIRDYAPLPGRSLAAGLRY
                     EF"
BASE COUNT         1138 a         2531 c         2637 g         1185 t
ORIGIN      
        1 cacctcgttc caggtggccc ggtccaggtg cacctcgcgg atcatctccc cgcccccctg
       61 caccggctcc agctcctcca ggagctccgg ggcggccacc aggaacccca ccccggtggg
      121 gccgtaggcc ttgtgggcgg agaaggccaa gaagtccggc aaggtcgtgg cgctgcaggt
      181 gcagcgtggc gaccggcagt tcttcatcgc catccggtcg gaatagcgcc ctttccaccg
      241 cgcgaggggg ccggagtcgc atccggcccc gtccttcggc tcgtgtatcc ttgcgggctc
      301 cctttccctg atcccgcgca tgcaagaacg atacgaaccg gctgccgtcg agggcagcgc
      361 ccaggcggcc tggcaggccg cgaacgcctt cgccgcggat gaatccgcgc ccggcgagaa
      421 gtactactgc ctgtcgatgt tcccgtaccc gtcggggcgg ctgcacatgg gccatgtgcg
      481 caactacacg atcggcgacg tgctggcgcg cttcatgtac atgcgcggcc gcaatgtcct
      541 gcagcccatg ggctgggatg cgttcggcct gccggccgaa aacgccgcga tggcgaacgg
      601 cgtgccgccg gcgaaatgga cccgcgagaa catcgcgtac atgaagcgcc agctccagtc
      661 gctcggtttc gcgatcgact gggagcgcga ggtcgcgacc tgcgatccgg cttactaccg
      721 ctggaaccag tggctgttcc tgcggatgct cgagaagggc atcgcctaca agaagacggg
      781 tgtcgtcaac tgggatccgg tcgaccagac cgtgctcgcg aacgagcagg tcatcgacgg
      841 gcgcggctgg cgcacgggcg cgccgatcga gaagcgcgag atcccgatgt actacctcaa
      901 catcacggcc tacgccgatg agttgctcgg cgcgctcgat ggcctcgacg gctggcccga
      961 tcgcgtgagg acgatgcagg ccaactggat cggcaagagc gtgggctgcg agatcgcatt
     1021 cccgtatgcc gacgacacga gcgcgctgat gggcagtgcc ggcgcgctca aggtgttcac
     1081 gacccgcgcc gacacgttgt tcggcgcgac cttcatggcg atcgccgccg agcacccgat
     1141 cgcgctcgcg gcggccgggc gcgatgcgaa gctcgctgcg ttcgtcgacg aatgccgtcg
     1201 cggcagcacc atggaagccg acgtcgccac ccaggaaaag aagggcctgc cgaccggcct
     1261 gcacgtatgt cacccgttca ccggcgagcg cgtcggtatc tggatcgcga actatgtgct
     1321 gatgggttat ggcgagggcg ccgtcatggg cgtaccggcg cacgacgagc gcgacttcga
     1381 gttcgccggc cggcacggca ttccggtggt cacggtcgtg aggtcgacgc gcggcgccta
     1441 cgacgaggtc gtcgcgccgt ggatcgatgc ctacggcgag ccgggcatca cggtcgcttc
     1501 cggcgaattc accgggctcg agtccgatgc cgcggtcgat gcgatcgcgg caaaactcga
     1561 ggaacgcggg ctcggcgcga agcgcgtgca gtaccggctg cgtgactggg gcatttcgcg
     1621 ccagcgctac tggggctgcc cgattccgct gatccactgc cccgcctgcg gcgacgtacc
     1681 ggtgcccgac gcggacctgc ccgtggtgct gcccgagcac ctggtgccgg atggcagcgg
     1741 caatccgctc gcccggacgc cggagttcgt caattgcagc tgcccgaagt gcggtgggcc
     1801 ggccaggcgc gagacggaca cgatggacac gttcgtcgac tcctcatggt atttcctgcg
     1861 cttcgccagc gccgggaacg accaggcgat ggtcgatgcg cgtgccgagt actggctgcc
     1921 cgtcgaccag tacatcgggg gcatcgagca cgcgatcctg cacctgctgt actcgcgctt
     1981 ctggacccgc gtcatgcgcg acctcgggct ggtcaggatc gacgagccgt tcgcccgcct
     2041 gctgacgcaa ggcatggtgc tgaaccacat ctactaccgc caggaggccg gcgcgcgccg
     2101 cagctacttc aatccggcgg acgtcgagat gcggggcggg caggccgtgc tgctcgccga
     2161 tggccagccg gtcgtgcacg atggcctcgg cacgatgtcg aagtcgaagg gcaatggcgt
     2221 cgacccgcag cagctcgtcg aggagttcgg cgccgacacc gcgcgtcttt tcatgatgtt
     2281 cacggctcct ccggagcagt cgctcgagtg gagcgacgag ggcgtgcagg gcgcattccg
     2341 gttcatccgc cgcctctgga aggcggtgca cgacctggtg tcgcaggggc cgctgcccga
     2401 cgccgacttc gcggtgctca ctgccccgca gcgcgcgctg cgccggctga cccaccagac
     2461 gctcgccaag gtcaccgacg acatcggtcg ccgccatacg ttcaacacgg cgattgccgc
     2521 ggccatggaa ctgctgaatg gcgcgacgcg catcgatacc agcgaggccg cgggccgggc
     2581 ggtcgcgcgc gaggcgctcg aagtgatcgt gctctcgctc tcgccgatca tcccgcacgt
     2641 gacgcacgtg ctgtggcatg cgctcggcca tgagcgcgcg ctgatcgagg agccatggcc
     2701 ccagcccgat cccgccgcgc tcgcgcgcga cgaggtcgag ctcgtcgtgc aggtaaacgg
     2761 caggctgcgc ggcaggatca ccgtggccgc ggaggcctcc gacgaggtgg tgcgcgcggc
     2821 agcgctcgcc gaggagaatg cgcgcaagtt catcaacgat gcgcccgtgc acaaggtgat
     2881 cgtcgtgccc ggcaagctcg tcaacatcgt ggtgtaggcg gtgcgacgtg ccatcgcggt
     2941 ggtctcggtc gcgctcgccg cagcggcgtt gtcgaccgcg tgcggcctgc ggctgcaggg
     3001 gcgcgaggcg acgccccagg ccttcgcgag cgcgcacgtc gaggccccgg accagcagag
     3061 cgatttcgtg caggcgctca cgcgctcgct gatggcgagc ggcgtgcgcg tcgagcgcga
     3121 cgcggcgcgt gccgaagcgg tgatccgcat ctcgcgcgac caggtgcgcg agcatgtgct
     3181 gtcggtctcg gcacgcaaca tcccgcgcga atacgagctg acctacgggg tcgagttcag
     3241 cgtgcgcagg ggcgaagaag agctgctcgc gccgcaggtc gccgaactca cgcgtgactt
     3301 cacgttcgat gaaacgatcc tgctcgccaa ggagcgcgag cagcgcatgc tcgaggaagc
     3361 gctggcccgc gatctggcgg cgatcgtgat gcgccagctc gccgcgctct gagccggtgc
     3421 ccgtctccgc cgcggtactg cgcgtcaacg gcaccgaccg ggtcgcgctc ggtctgctgc
     3481 tcgcgcgttt cggcatggac ctgcgcctcg tcgcccacgg cgaggtcatc ccgggctcct
     3541 actggggcga gagcgaagcc ggactgatcg gcgatcgcct gtacgcgcgc ctcgataccc
     3601 cgctgcattc ggtgctgcac gaggccgccc actatgtctg catgacgccg gagcggcgca
     3661 cgggcctcga tcgcgacgcc ggtggcgacg acgccgagga atgcgccgtg tgctacctgc
     3721 aggtgctgct cgccgatgcg ctgcccggcg tcggccggga gcggctctgc gacgacatgg
     3781 atgcctgggg ctacagcttt cgcgaaggcc cgacgcgcgc ctggctcgcg ggtgacgcgc
     3841 gcgatgcgcg cgaatggctg ctggcccgcg acctgatcga tcgggatgac cggccgacct
     3901 ggcggctgcg cttcagcgat ccgcgaagcg gtccgtcgcc ctgacgagct cgtggatgtt
     3961 gcccggctcg aacgcggaat ggcccgcgtc gggcaccacg cggaagtcgg cctcgggcca
     4021 cgcgcgatgc aggtcccatg cgctcttcat cgggcacacg acgtcgtagc ggccgtgcac
     4081 gatcaccgcg gcaatgtggc ggatcttcgg cacgtcgtcg agcagctgtg tgggcgaacg
     4141 caggaagccg cggttgacga aatagtggca ttcgatgcgt gcgaaagtct cggcgtaggc
     4201 gtcttcctcg aacttcttca cttcgcccgg atcgaagagc aggtggctgg tggcgccttc
     4261 ccagatcgac cagctgcgtg ccgcgctcgc gcgcacctgc gcatcatcgc tcgtcaggcg
     4321 gcggtagtac gcgtccatca tttcgctgcg ttcggcgggc gggatcgcgg cggcgaactc
     4381 ggcatacagt tcggggaaca gcgcgcccgc gccatcggga tggccgtaga accactcgag
     4441 ctcccacggt cgcagcagga agatgccgcg cagcacgagt tcggtgacct ggccgggatg
     4501 cgcctgcgca taggcgaggg cgagcgtgga accccatgag ccgccgaaaa cctgccagcg
     4561 ctcgatgccg aggtgttcgc gcaggcgctc gatgtcgttc accaggtgcc aggtggtgtt
     4621 gtcctcgagg ttcgcgcgcg gacggctctt gccgcagcct cgctggtcga acagcacgat
     4681 ccgatagcgc ttcgggttga agaagcggcg catcttcggg tcggtgccgc cgccgggccc
     4741 gccgtgcagg aatacgaccg gcttgccgcg cggattgccg ctttcctcga aatagagcgt
     4801 gtgggtcggc gagaccgcga ggcgcccggt gcggtaggcc tcgatcgggg gatacagctc
     4861 gcgcaaggcg ccgggtgcgg gcttcggtcc gccgcgtgtg ccggcggcgc gcttgtcggt
     4921 agcgcgcttt ttcggcgccg cgggccgtct cgccagggcg ggtttgcgct ttgccgcagt
     4981 cttcttccgc tttgccatgc ctttgccctc aggagcccgg cgagaccatc gagccgagtg
     5041 gtgcgccgcc gagcagatgc aggtggaggt ggtacacctc ctgtccgccg tgccgattgc
     5101 agttgatgat gagccggtag ccgtcctcgg cgatgccttc gcgccgcgcg agatcgcgcg
     5161 cgaccgtgaa catgtgaccg atgagagtct cgtcctcgtc ggcgatgtca ttggcggtgg
     5221 gaatcggctt cgacggaacg atgaggatgt gcaccggtgc gcgcgggtgg atgtcgcgaa
     5281 atgccgtgac ccgctcatcc tggtgcagga tggtggcggg cagctcgcca gcgatgatcc
     5341 gttcgaacag ggtcggcatt gcgtgcgctc ctcgtcgcgc cgggtggatg cacagcggca
     5401 ttctgacata cagggccggt ctggcggcgg ccggcctcag aattcgtagc gcaggcccgc
     5461 cgccagcgag cgtcccggca gcggcgcgta gtcccggatg ggcgaggacg cgcggcgcgc
     5521 ctcctcgtcg gcgagattgc tgcctttcag gaacgcgacg atctcgccgc cgcccacggg
     5581 ctggtgccag gacacgtcgg cgtcgatcat cgtgtagccg ttcgtcggca gctcgttcgt
     5641 cgcgacatcg tcctggtccg catacctgaa gatcgacagg cccgcttgcc acacgccgcg
     5701 ctcgaagccg agctcggcgc cgaagcgcca gggcggcatc agcggcaggt tgccgccgcg
     5761 atcgggcttg ccacggacat agtcggtcgc aaggcgcagc gtgagggccg cagcatcgcg
     5821 cgcgacgagc ggcaaggtca cctcggcctc caggccgtgg aagctcgaat cctcctggac
     5881 ataatcgacg acggggaaca gctcgccttc ctcggggtcc tcgaagaact ccccggtcgg
     5941 gcgcgcatgg atgtagccat cgtaacggct gtagaaggca cccagcgtga actgcacggc
     6001 gccggcgtac ttgcgcagtg tcaggtcggt ggtcagcgcc gcctcttcgc cgatcgcaga
     6061 gtcgccccgc tcgtagcggc cgagcgccac gtgcacgccg tcggcataca gctcggtggc
     6121 gccgggatga cgggtggcac gggtgaagtt cagtgccacc gagtactccg gggcgaactt
     6181 ccagcgcaat cccgcggacc cactcacgcc gtcgcccgag tagtcgggca ggccggcctc
     6241 ccgctcagcc tcgatgcgct ggtcctcgta gcgcagcgcg agttcgacgg tcaatggccc
     6301 gaccggtcgc tcctcgaaca cgaacaaccc gacgttacgc atcgagttgc cgggcacgaa
     6361 cgcttcctcg ccgagcgcgc ggaaatcgag ctcgcgatac tgcatgccga aggttccgcg
     6421 ccagccgccg agcacggcat ggtcgaacga cagccgcgcc tcgccggagt cctgggcgaa
     6481 ggtcgtgcct gcctcgccgt ccggttcgaa ctcctggtga aaatactcgc tgtgtgccgc
     6541 gcgcaggcgc atcgaatcaa tgcccgtgaa cggatcgtcg agcccaccgc tcaggtcgta
     6601 gcgctcctgc gtcatgtcga tgcgtacgct cgaaccgtcg gccccggatt ccggcccggg
     6661 tgtgccgtaa ttcgcgtcgg tgcggctcgc cgccacgccg aggaagccgc gggaggcgag
     6721 gtagctcacg ccgagtgcgc cgccgtcgct gtcgccgtcg ctgccgtaga gggtgccgtg
     6781 cgggttgacg ggatccgtct cgccctgggc ggcgagttgc tccgcgaggc gctgcgagag
     6841 cgcgttgccc ggtatgtcga tgtcatcgct cgtgcgccga tatccgtccg cgtgcagcac
     6901 gaagtccccg gcccgcacgt cgagcctgcc ggcgccggaa gtttccgaag cggccgaatt
     6961 gccgcgcagt tcgacggcgc cctgcagggc ctgcgccagc ggcttctcgg cgaaacggct
     7021 cgtcacgacg ttcacgacgc cacccaccgc ggcattgccg tgcaggagcg tcccgggtcc
     7081 cttgatcact tcgatgctgc gcgcaaccag tggatcgatc gagacggcgt ggtcctcgct
     7141 gagcgccgag acatcctgcg tgccgaggcc gtcctcgagc atcgacacac gctcgccgcc
     7201 gaggccgcgg atcaccgggc ggctcgcgac cggcccgaac cacgtgccgg tgacgcccgc
     7261 ctgcttcgcg agcgtctcgc cgagactctg gccgagttcg cggtggagcg catcgccgcc
     7321 aagcacgatg acgggctggg cggtttcgag gacgccctgg cgcagggcgg acgccgtcac
     7381 cacgacttcc tcgagcgcgg gtggcgccgc gagcgtggcc gggatgacgg gaatgagcaa
     7441 cacggctgcg cagtgcagcc cgcggatacg ggaaaggaaa agcatgaggg c
//
LOCUS       DAOQVY010000120         7398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_11035_length_7398_cov_6.317309, whole genome shotgun sequence.
ACCESSION   DAOQVY010000120 DAOQVY010000000
VERSION     DAOQVY010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11035_length_7398_cov_6.317309"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="PKN91_10405"
     CDS             complement(<1..1145)
                     /locus_tag="PKN91_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28520.1"
                     /translation="MLPIRAGALALAVFAMGAAGADAPLAAELADAAELPQYTLEVRI
                     EPDTHHLAVDATLGFPAQYAGRDVEFLLAAPLAITASTPAIERLPAGDTGGFTGINGS
                     SAALEKSGRAARYRVTLPKDSPVLRLSYAGAVDFGFDTPEEEYARGFSETAGVIGAKG
                     VYLAGSSLWYPYLGDTLFVPTVHATAPEGWHLITVDPAPVDELPLVGGPLTRYAESVG
                     AVEAQVFLRTPDEALARKYLDATARDLEMYRQLIGPYPYSKFALVENFWETGYGMPSF
                     TLLGPQIIRFPFILTSSYPHEILHNWWGNSVFVDYATGNWCEGLTAYMADHLFKEQLG
                     QGAEYRRDTLKRYRDYVKDAHDFPLNEFRSRHSAATEAVGYGKTLMG"
     gene            complement(1151..2386)
                     /locus_tag="PKN91_10410"
     CDS             complement(1151..2386)
                     /locus_tag="PKN91_10410"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HNS28521.1"
                     /translation="MAHEILRPLVVAACWLLASMPAAAGENILDLPIGDASRAARVVG
                     VTLDGITDTRTGELITPAELARRLATTRVLFIGEEHTTPEFHDVQLATLKALRESGRE
                     VLIGLEMYPYDQQAPLDQWIDGKLTERGFVDLSGWYVYWSHHWGYYREIFDYAREQGL
                     RMYGVNLPRAAVNLAREKGFEALDPEARRHLPPKIDLTNAEHRRLFRAYFADDDALHS
                     QMSEAALDSLYRAQVTWDASMGWNAAQALEQHGGKNAIMVVLIGSGHVAYNLGGVRQL
                     AANYDGEIRSLIPVAVRDKDSGPVGSVRASYADFIWGVPGRDGPGLPVLGVSLAGRIG
                     KEPSKVIQVDKDGSAAQAGVKVGDVLVSLGDTPIANTTDLQRATGEYAWGDDIALAIR
                     RDGEPLTLDLVFRRPTPTK"
     gene            complement(2368..4857)
                     /locus_tag="PKN91_10415"
     CDS             complement(2368..4857)
                     /locus_tag="PKN91_10415"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNS28522.1"
                     /translation="MNRFHLVTGLAAASFLGLNTSVVAQTKTDSLDEIVVTARKAEER
                     LIDVPLAITALSETIIEERGIRNLDDVAANTPGLQFSNVIGEFLPSPVIRGVAPIDIL
                     GENNTAIFIDGIFVSAREGLNFSQLDLERIEVVKGPQAAMYGRNSFSGAINYVTAKPS
                     DTFRGKTEVTWGNDGRLLASLSASGPLIEGVLKGRAAVLYDNFDGSYKNQWAGPGPGP
                     DIGGYQYKTFQGSLLWTPTDNFEGTLGLYVSDDQIDDSALSAVPSNCENRNQVDPMLS
                     SRLLNYCGEFPSVGKNDLSKIAGATGEDRDVTRVTMKLEWSLGNGGTIDSLSGYSNVS
                     QSYFVDGGRGGGNDQNFTYLASPIIPRFFGPPPAFNSSATRAQFSTGLLQIGPGGSTE
                     EFSQELRYSSDPTKRFSWSVGAYYYDTTSKASLDGVIANTPLPEDFGSFCLACTNLGP
                     PFNWIDFATGAGDAAFLPWFTSATGDAIAGNFFQNDTEAPSGFVHVEFDLTEKLSWGA
                     EARYTEEEKTATTRLPNTPVSSRKVKSELVNWRTNLRYQPHDSLTFYGAVAHAEKGGG
                     SDSASVEFQDAPSVSEAVVVGFDPEDLLSYELGMKSEFLDRRLSLEVDLYYMDWSEIV
                     IPQVLESIDGRDLLIPTAFSINGGDASIKGAELAITAHPVAGLDLNLGLAYIDGQYDD
                     AKIESFVQFPSFAPDGDVSGNQILRQSEWQWNVGAGYSAVARGDVNWFFRTDASYRGE
                     QFADSSNQAILPESLLVNASIGLRADRWSLELWGRNLTDEDAPTGAFRDVYFTNTLPD
                     GTTGGGTFFPLRYTISHPRLTTYGLTWRMKF"
     gene            4955..6067
                     /locus_tag="PKN91_10420"
     CDS             4955..6067
                     /locus_tag="PKN91_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28523.1"
                     /translation="MRNTYLVVVIALAACSQGKDAATTATDAPAPATAATLAPVEVPA
                     GAAVSTREGELPSWQIPNMGEAAEAYYGPDSLHLIAQVRNSKAIASQRGGEGFLTTTF
                     TDTGEDIVLVNDKGYDACSYFFPDGERLIWTSVRDNMGDGKTLDGDWSDWEHYPQGAE
                     LYISNRQGGDIQRLTNNNYYEAEVTVSPDGKWIVFGRQIEGNMDLWVMNADGTGEKQI
                     TFTEDWQEGAPYFLPDSETILFRAWKASEYGQRPTPMTIFTIKRDGTGLEPQTHTHEM
                     NWAPYPAPDGKHFVYVRIEPNSNNWEVYLGEIGAGPDAAPRRLTFDDGFDGFPSISPD
                     GRKLVWTTNRSDESKAFMQNLRLHVMDVSSLDLGPK"
     gene            6093..>7398
                     /gene="aspS"
                     /locus_tag="PKN91_10425"
     CDS             6093..>7398
                     /gene="aspS"
                     /locus_tag="PKN91_10425"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNS28524.1"
                     /translation="MRTHFCGQVNEFSSGKPVTVAGWVHRRRDHGGVIFVDLRDRSGL
                     VQVVFDPDAQDVFATAEKLRNEFVVRVTGLVRERPPGTANPNLDSGSIEILAHELEIL
                     NRAEPMPFQLDEQVSEEVRLRYRYIDLRREIMAKRIRLRHAATRAMRQFLDEAEFVDI
                     ETPMLTKATPEGARDYLVPSRTHPGKFFALPQSPQIFKQLLMVAGFERYYQIVRCFRD
                     EDLRADRQPDFTQLDIETSFLTQDEIMALMEGLIRHVFKEATGVALPDPFPRLSYAEA
                     MRRYGSDKPDLRNPLELVDVADLVRDCDFKVFAGPAKDPRGRVAALRVPGGAAMPRSQ
                     IDEYTAYVARYGARGLAYVKVNDRAAGREGLQSPIIKFLSDAAVAGILERTGAADGDL
                     LFFGADSAKVVCDALGALRLKVGVDLGLVAEGWRPLWVVDFPM"
BASE COUNT         1242 a         2345 c         2531 g         1280 t
ORIGIN      
        1 aagcccatca gcgtcttgcc gtagccgacc gcctcggtgg ccgcgctgtg gcgcgaacgg
       61 aattcgttca gcgggaagtc gtgcgcatcc ttcacgtagt cgcgatagcg cttgagcgtg
      121 tcgcggcggt attccgcgcc ctgacccagt tgttccttga acaggtggtc ggccatgtag
      181 gcagtgaggc cctcgcacca gttgccggtg gcgtagtcga cgaagaccga gttgccccac
      241 cagttgtgca ggatctcgtg cggataggac gacgtgagaa tgaacgggaa gcggatgatc
      301 tgcgggccga gcagcgtgaa cgagggcatg ccgtagcccg tttcccagaa gttctccacg
      361 agcgcgaact tcgagtaggg gtaggggccg atcagctggc ggtacatttc gagatcgcgc
      421 gccgtggcgt cgaggtactt gcgggcgagg gcctcgtccg gcgtgcgcag aaacacctgt
      481 gcctcgaccg cacccacgct ttccgcgtag cgcgtcagcg ggccgccgac cagcggcagt
      541 tcgtcgaccg gcgccgggtc gacagtgatc agatgccagc cctcgggcgc cgtggcgtgc
      601 accgtgggca cgaacagcgt gtcgccgagg taggggtacc agagcgagct gccggcgaga
      661 tacacgcctt tcgcaccgat cacgccggcg gtttcactga agccgcgcgc gtattcctcc
      721 tcgggcgtgt cgaagccgaa atcgaccgcg ccggcatagc tgagccgcag gaccggcgag
      781 tcctttggca acgtgacgcg gtagcgcgcc gctcggccgg acttctcgag cgcggcgctc
      841 gagccgttga tgccggtgaa cccgcccgtg tcgcctgcgg gcaggcgctc gatcgcgggc
      901 gtcgaggcgg tgattgcaag tggcgccgcg agcaggaact cgacgtcgcg gcccgcgtac
      961 tgcgcgggaa aaccgagcgt ggcatcgacc gcgaggtgat gcgtgtccgg ttcgatgcgc
     1021 acctcgagcg tgtactgcgg cagttcggcc gcgtcggcca gctccgcagc gagtggggca
     1081 tcagcgccag cggcgcccat ggcaaagacg gcgagtgcga gtgcacccgc ccggatcggc
     1141 agcatgtttt tcattttgtg ggtgtcggtc gtcggaaaac gaggtcgagt gtcagcggct
     1201 caccgtcgcg gcgaatggcg agggcgatgt catcgcccca ggcgtattcg ccggtggcgc
     1261 gctgcagatc ggtcgtgttg gcgatgggtg tgtcgccgag gctcaccagc acatcgccca
     1321 ccttgacgcc ggcctgcgcc gcactgccgt ccttgtcgac ctggatgacc ttcgagggtt
     1381 ccttgccgat ccggccggcg agcgagacac cgagcacggg caggccaggg ccgtcgcggc
     1441 ccggcacgcc ccagatgaaa tcggcgtagg aagcgcgtac ggagccgaca ggccccgaat
     1501 ccttgtcgcg caccgccacc ggaatcagcg aacggatctc gccgtcgtag tttgccgcga
     1561 gctggcggac gccgccgagg ttgtaggcga cgtggcccga gccgatcagc accaccatga
     1621 tcgcgttctt gccgccgtgc tgctcgagcg cctgtgcggc gttccagccc atcgacgcat
     1681 cccaggtgac ctgggcgcga tacagcgaat cgagcgcggc ctcggacatc tgcgagtgca
     1741 gggcatcgtc atccgcgaag taggcgcgaa acagccgccg gtgctcggcg ttcgtcaggt
     1801 cgatcttcgg cggcaggtgc ctgcgcgctt cggggtcgag cgcctcgaag cccttctcgc
     1861 gcgcgaggtt gaccgccgca cgcggcaggt tcacgccgta catgcgcagg ccctgctcgc
     1921 gggcatagtc gaaaatttcg cggtagtagc cccagtggtg cgaccagtag acgtaccagc
     1981 cggacaggtc gacgaagccg cgctccgtga gcttgccgtc gatccactgg tcgagcgggg
     2041 cctgctggtc gtaggggtac atctcgaggc cgatcaggac ctcgcggccg gattcgcgca
     2101 gcgccttgag ggtggcgagc tgcacgtcgt ggaattcagg agtggtgtgc tcttcgccga
     2161 tgaagagcac gcgggtggtg gccaggcggc gggcgagttc cgccggcgtg atcagctcgc
     2221 cggtgcgggt gtcggtgatg ccgtccagcg tgaccccgac cacccgggcg gcgcggcttg
     2281 cgtcgccgat cgggaggtcg aggatattct cgccggccgc cgccggcatg ctggcgagga
     2341 gccagcatgc ggcgacgacg aggggtctca gaatttcatg cgccatgtga gtccataggt
     2401 ggtgaggcgt ggatggctga tcgtgtagcg gagcggaaag aaggtcccgc cgcccgtcgt
     2461 gccgtccggc agcgtgttgg tgaagtacac atcgcggaag gcgccggtcg gggcatcttc
     2521 gtccgtcaga ttgcggcccc acagctcgag cgaccagcgg tcggcgcgca ggccgatcga
     2581 ggcattcacc agcagcgact cgggcaggat ggcctggttg gacgaatccg cgaactgctc
     2641 gccgcgatac gaggcgtcag tgcggaagaa ccagttgaca tcgccgcgcg ccactgcgct
     2701 gtagcccgcg cccacgttcc actgccattc ggactggcgc aggatctggt tgcccgatac
     2761 gtcgccgtcc ggcgcgaagc tggggaactg gacgaaggat tcgatcttgg cgtcgtcgta
     2821 ctggccatcg atgtaagcca ggccgaggtt caggtcgagg ccggcgaccg ggtgggcggt
     2881 gatggccagc tctgcgccct tgatcgaggc gtcaccgccg ttgatgctga acgcggtcgg
     2941 aatcagcagg tcgcggccgt cgatcgactc gaggacctgc gggatcacga tctcggacca
     3001 gtccatgtaa tagaggtcga cctcgaggct caggcggcgg tcgaggaact cgctcttcat
     3061 gccgagttcg tagctgagca ggtcttccgg atcgaagccg accaccaccg cctccgagac
     3121 gctgggagcg tcctggaatt cgacgctcgc ggagtcggag ccaccgccct tttcggcatg
     3181 cgcgacggca ccgtagaagg tgaggctgtc gtgcggctgg tagcgcaggt tggttcgcca
     3241 gttgacgagt tcggatttca ccttgcgcga gctgaccggg gtgttgggga ggcgagtagt
     3301 ggcagtcttc tcttcctcgg tgtaacgggc ttcggcgccc cacgagagct tctcggtcag
     3361 gtcgaactcg acatgtacga agcccgacgg tgcttccgtg tcgttctgga agaagttgcc
     3421 ggcgatcgcg tcgccggtcg cgctcgtgaa ccagggcagg aaggccgcat cgccggcgcc
     3481 cgtcgcaaaa tcaatccagt tgaacggtgg gccgaggttc gtgcatgcga ggcagaagga
     3541 accgaagtct tccggcagcg gcgtgtttgc gatcacgcca tcgaggctcg ccttgctggt
     3601 ggtgtcatag tagtaagcgc cgaccgacca gctgaagcgc ttggtcggat cgctcgagta
     3661 gcgcagctcc tggctgaact cctcggtcga cccgccgggc ccgatctgca gcagcccggt
     3721 ggagaattgc gcgcgcgtcg ccgagctgtt gaatgccgga ggcgggccaa agaagcgtgg
     3781 aataatcggt gatgccaggt aggtgaagtt ctggtcgttg ccgccaccgc ggccgccgtc
     3841 gacaaagtac gactggctga cgttggaata gccactcagc gagtcgatcg tgccgccgtt
     3901 gccgagcgac cattccagct tcatcgtgac gcgcgtgacg tcgcgatcct cgccggtcgc
     3961 gcctgcaatc ttgctcaggt cgttcttgcc gaccgacggg aactcgccgc agtagttcag
     4021 caggcggctc gacagcatcg gatccacctg gttgcggttc tcgcaattgg acgggaccgc
     4081 gctcagcgcc gagtcgtcga tctggtcgtc cgatacgtag aggcccaggg tgccctcgaa
     4141 gttgtcggtc ggtgtccaca gcaggctgcc ctggaaggtc ttgtactggt agccgccgat
     4201 gtcggggccc gggccggggc cggcccactg gttcttgtag gagccgtcga agttgtcgta
     4261 cagcaccgcc gcacggccct tcagcacgcc ttcgatcagc gggccgctcg ccgagaggga
     4321 tgcgagcaat ctgccatcgt tgccccaggt gacctcggtc ttgcccctga aggtgtcgct
     4381 cggcttcgcg gtcacgtagt tgatcgcgcc gctgaagctg ttgcggccgt acatggccgc
     4441 ctgcgggccc ttcaccactt cgatgcgctc gagatcgagc tggctgaagt tgaggccttc
     4501 gcgcgccgac acgaagatgc cgtcgatgaa gatcgcggtg ttgttctcgc ccaggatgtc
     4561 gatcggcgcg acgccgcgga tcacgggcga ggggaggaac tcgccgatca cgttggagaa
     4621 ctgcagtccc ggcgtgttgg cggcaacgtc gtcgaggttg cggatgccgc gctcctctat
     4681 gatggtctcg gagagggcgg tgatggcgag tgggacgtcg atcaagcgct cttcggcctt
     4741 acgggccgtg acgacgattt catcgaggct gtcggtcttg gtctgggcca cgacagatgt
     4801 attgagaccg agaaacgacg ctgccgccag tccggtcacg agatggaatc tattcattgt
     4861 tctccccgtg gacgcgccat gcgcccccct tgctctgtac gctaacgcgg cagcacactt
     4921 gtgatcaata tcaagaggga cggagacgac acaaatgcgc aacacatacc tcgtggtggt
     4981 catcgccctg gccgcatgct cgcagggcaa ggacgccgcg acgaccgcca cggatgctcc
     5041 cgcaccggcc acggccgcca cgctggctcc cgtcgaggtc cccgcgggcg ccgcggtgtc
     5101 cacgcgggag ggggaactgc cttcctggca gatcccgaac atgggcgagg ccgccgaggc
     5161 gtactacggc cccgacagcc tccatttgat cgcgcaagtg cgcaattcca aggccatcgc
     5221 gagccagcgc ggcggcgaag gcttcctgac cacgaccttc accgataccg gtgaggacat
     5281 cgtgctcgtg aatgacaagg gctacgacgc ctgctcctat ttcttccccg acggcgagcg
     5341 cctgatctgg acctcggtgc gggacaatat gggcgacggc aagactctgg acggagactg
     5401 gtccgactgg gagcactatc cgcagggcgc ggagttgtat atatccaaca gacagggcgg
     5461 cgacatccag cgcctgacga acaacaacta ctatgaggcc gaggtcaccg tttcgccgga
     5521 cggcaaatgg atcgtcttcg ggcgtcagat cgaaggcaac atggacctgt gggtcatgaa
     5581 tgccgacggc accggcgaga agcagatcac gttcaccgag gactggcagg agggtgcccc
     5641 ctacttcctc ccggacagcg agacgatcct gttccgtgcc tggaaggcct ctgagtacgg
     5701 ccagaggccg actccgatga cgatcttcac gatcaagcgt gacggcaccg ggctcgagcc
     5761 gcagacgcac acgcatgaaa tgaactgggc gccatacccg gcgccggacg gcaagcactt
     5821 cgtgtacgtg cgcatcgagc cgaactccaa taactgggag gtctacctcg gtgagatcgg
     5881 cgccggtccg gatgcggcgc cgcgccgcct gaccttcgac gacggtttcg atggcttccc
     5941 gtcgatctcg ccggacggcc gcaagctcgt gtggaccacc aaccgcagcg acgagtcgaa
     6001 ggcgttcatg cagaacctgc ggctgcatgt catggacgtg agctcgctcg acctcggccc
     6061 caagtgaagc ggatccggcg taaaatggcc ggatgcgtac acacttctgc ggacaggtaa
     6121 acgagttttc ttcgggcaag ccggtcacgg tggccggctg ggtgcacagg cgacgggatc
     6181 acggcggcgt gatcttcgtc gacctgcgcg accgctcggg cctcgtgcag gtggtcttcg
     6241 atcctgatgc gcaggacgta ttcgcgaccg ccgaaaagct gcgcaacgag ttcgtcgtac
     6301 gtgtcaccgg cctcgtgcgc gagcgcccgc cgggcaccgc caaccccaac ctcgattccg
     6361 gcagcatcga gatcctcgcg cacgaactcg agatcctgaa tcgcgccgag ccgatgcctt
     6421 tccagctgga tgagcaggtc tccgaagagg tgcgcctgcg ctatcgctac atcgatctgc
     6481 gccgcgagat catggcgaag cgcatccggc tgcgccatgc cgccacgcgc gccatgcgtc
     6541 aattcctcga cgaggcggaa ttcgtcgaca tcgagacgcc gatgctgacc aaggcgacgc
     6601 cggaaggcgc gcgcgattac ctggtgccct cgcgcaccca tcccggcaag ttcttcgcgc
     6661 tgccgcaatc gccgcagatc ttcaagcagc tgctgatggt tgcgggcttc gagcgctact
     6721 accagatcgt gcgctgcttc cgcgacgagg acctgcgcgc cgaccgccag cccgacttca
     6781 cgcagctcga catcgaaacc tcgttcctca cacaggacga gatcatggcg ctgatggaag
     6841 ggctcatccg gcacgtcttc aaggaagcga ccggtgtcgc gctgcccgat ccgttcccgc
     6901 gcctctccta tgcggaggcg atgcgccgct acggctccga caaacccgac ctgcgcaatc
     6961 cgctcgaact ggtcgacgtg gccgacctcg tgcgcgattg cgacttcaag gtgttcgcgg
     7021 gcccggcgaa ggacccgcgc ggccgcgtgg cggcgctgcg cgtgcccggc ggggcggcga
     7081 tgccgcgctc gcagatcgac gaatacacgg cttacgtcgc gcgctacggc gcgcgcggac
     7141 tcgcgtatgt gaaggtcaat gatcgcgccg cgggccgcga gggcctgcag tcgccgatca
     7201 tcaagttcct gtccgacgcg gcggtcgccg gcatcctcga gcgcactggc gcggcggacg
     7261 gcgacctgct gttcttcggc gccgattccg ccaaggtcgt gtgcgacgcg cttggcgcac
     7321 tgcgcctgaa ggtcggcgtg gatctcgggc tggtcgcgga gggctggcgc ccgctgtggg
     7381 tcgtcgactt cccgatgt
//
LOCUS       DAOQVY010000121         7253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_11330_length_7253_cov_5.064046, whole genome shotgun sequence.
ACCESSION   DAOQVY010000121 DAOQVY010000000
VERSION     DAOQVY010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11330_length_7253_cov_5.064046"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PKN91_10430"
     CDS             complement(<1..398)
                     /locus_tag="PKN91_10430"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS28525.1"
                     /translation="MSGLKILHVLDHSVPLQSGYAFRTLAILREQRRIGFETVHVTST
                     KHYGASADEEEVDGLTFFRTRPADSLLRRLPVVNQAMVSLDTATRVEEIARRLGVDVI
                     HAHSPALNGLAALKAGRRLGLLVVYEMCAS"
     gene            complement(395..2293)
                     /locus_tag="PKN91_10435"
     CDS             complement(395..2293)
                     /locus_tag="PKN91_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidotransferase 1, exosortase A
                     system-associated"
                     /protein_id="HNS28526.1"
                     /translation="MCGLTGIVDLRGRRELDRALLRRMNDSIQHRGPDGEGEHHEPGV
                     ALGHRRLSIIDIATGQQPLYNEDGSVVVVYNGEIYNYQSLIPELQALGHVFHTKSDTE
                     VIVHAWEAWGEDCVTRFRGMFAFALWDRNRETLFLARDRLGVKPLFYALLPDGQCVFG
                     SELKALLLHPGVTRTIDPCAVEEYFAVGYVAEPRTIYTSVRKLPPAHTLVIRRGETAP
                     APREYWDVRFSLDNRIGFEDAMAELDRRLQDSIRLRMISEVPLGAFLSGGVDSSAVVA
                     TMARISDAPVNTCSISFDDPAFDETKFAQMVADRYQTRHYVDQVASDDFGLIDTLVGL
                     YDEPYADSSAIPTYRVCELARKHVTVALSGDGGDESFGGYRRYQLHLMEEKLRSALPL
                     GFRRPVFGLLGRLYPKADWAPRVFRAKTTFESLARNSVEAYLQSVSIVRAPMRARLFS
                     GGMRASLGGYRVDDLFARHAARAGTDDPLALIQYLDLKTYLVGDINTKVDRASMAHSL
                     EVREPLMDHPLVEWLGTLPSSFKMRGGEGKRLLKKSMEPWLPHDVLYRPKMGFAVPLV
                     RWFRGPLRETVRTAVLGDTLRDTGLFEPRVLEEIVSAHESGRRDYSAPLWTLLMFEGF
                     LRRGAAAR"
     gene            complement(2297..3439)
                     /locus_tag="PKN91_10440"
     CDS             complement(2297..3439)
                     /locus_tag="PKN91_10440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03088 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNS28527.1"
                     /translation="MAEAPLIAHVIYRLDYGGLENGLVNLVNRLPRAKYRHAIVCLAG
                     FTDFRKRIQRDDVEVISIDKQPGKDPAAYGRLWRQLRRLRPAVVHTRNLGTVDLQWVA
                     LAAGVRHRVHGEHGWDAADPRGESPRALRIRRLCRPATQRYVAVSKDIERWLQERIKV
                     PPGRIRQIYNGVDVDRFSPEGPLPADLPWAVQGAERPLVIGTIGRLDPIKNQAALLEA
                     FARLAKAPEAPQGRLRLAIVGDGPEAGRLRERAQALGIAPLVWMPGMRPDAPALLRSF
                     DIFVLPSQNEGISNTILEAMASSRPVVAGQVGGNGELIEQGVSGTLYDPRDPAALAAA
                     IARYLDDGGLRAAHGAAGRERCLRAFAMDSMVQAYTGLYDGLLTDH"
     gene            complement(3444..4760)
                     /locus_tag="PKN91_10445"
     CDS             complement(3444..4760)
                     /locus_tag="PKN91_10445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNS28528.1"
                     /translation="MKISIFGLGYVGAVSMACLARDGHEVTGVDVDPLKLELIRSGKS
                     PIVEEGIQDLTRDVVASGKVKVTDDVAAAIQATELSFVCVGTPSSPNGSQDMSYIRRV
                     MESIGEALAKKSAFHTVVLRSTVQPGTLDELVRPTLEKASGKVADRDFGLAFQPEFLR
                     EGTSIKDYDRPPITIVGTRSTRAADALRAVFGHLPCEFVVTSVGVAEMQKYAANAFHA
                     VKITFANEIGRLASAVDVDAREVMALICKDTQLNISPAYLRPGFAFGGSCLPKDLRAL
                     TYMGKTHDVTTPMLGGVLGSNRAHIDQAIDLVMRGETRKVGMIGLSFKKGTDDLRESP
                     LVSVAERLIGKGYDLRIYDPEVNLSRLIGANKRFIEQSIPHIGKLMVGSCEEAVSHGD
                     TILVGLGGKDIEAALAATLEPRHAVVDVVGIAMRGGRAGRYSGICW"
     gene            complement(4757..5941)
                     /locus_tag="PKN91_10450"
     CDS             complement(4757..5941)
                     /locus_tag="PKN91_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNS28529.1"
                     /translation="MKVLFVCHRVPFPPKRGGKIRPFNIIRHLTDAGHEVTVASLARS
                     AAELEESRGLAQHCAEAIVEVIPDRVAWPQMVARLPTASPSSFGYFWSRRLARRIEAA
                     AKATRFNLVFVHCSSVAPYVERLAAPLKVLDYGDMDSQKWREYSGHRAFPLSMGYWLE
                     AVKLERRERQLADHFDLCTCTTRAELESLRALGVSTPSDWFPNGVDAGFFAPDGEGYD
                     TDLLAFVGRMDYYPNQQGVLGFCRDVLPALQAKRPAARFAIVGADPPEHIRALGKLPG
                     VTVTGSVPDVRPHVRRAALTIAPLAIARGTQNKILESMAMGVPVVCSPQASGGVDAVA
                     GEHLLVASGTDEWVAAIDRLLGDADARRRLAAAGRDRVLSHHSWAASMRRLDGLIEEA
                     GK"
     gene            complement(5938..>7253)
                     /locus_tag="PKN91_10455"
     CDS             complement(5938..>7253)
                     /locus_tag="PKN91_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNS28530.1"
                     /translation="LVAEEGRVAVNQYWRLDAGIDAARSEQSWIDGLRSELERSVREQ
                     MVSDVPIGAFLSGGIDSSAVVAFMSRHTDRPVRTYSIGFAGSSGAALYNELPHARAVA
                     RAFGTEHKEILVQPDVAALLPGLLSHMDEPVADAAFITTYLVSKFAREDVTVILSGVG
                     GDELFGGYRRYLDEHYRRQYQRIPRFIRRSMIDPLARRLPSDRHHRLLNELRLAKAFV
                     LADSLSFEERYRRYMEVFSADERAALLLGEPPAFDDCIARAFAASNATDPLRRLMDVD
                     LATQMPEDLLMLTDKMSMAVSLECRVPLLDQRLVEFAARMPGALKVRGGDLKYAMKRA
                     LAGILPDSILNREKRGFGAPIGAWFKQELAPLVADLLSRETILRRGLLDPDAVARTIA
                     EHEQQQADRTDHLLALVNLEVWARLYLDGASVAGVTEDLKAKLAA"
BASE COUNT         1196 a         2494 c         2441 g         1122 t
ORIGIN      
        1 caggaggcgc acatctcgta gaccacgagc agaccgagtc gcctacccgc cttgagcgcc
       61 gcaagcccgt tcagcgccgg ggaatgggca tggatcacgt ctacacccag acggcgggcg
      121 atctcctcga cccgcgtggc ggtatcgagc gagaccatcg cctggttgac cacgggcagg
      181 cgccgcagca gcgagtctgc cggtcgcgta cgaaagaacg tgagtccgtc gacctcttct
      241 tcgtcggcgc tcgccccgta gtgcttcgtc gacgtgacat ggacggtctc gaagccgatg
      301 cggcgctgct cgcgcaggat cgcgagcgtg cgaaacgcgt agccgctctg cagcggcacc
      361 gagtggtcga gcacgtgcag gatcttcaat ccgctcaccg ggccgccgcg ccccgtcgca
      421 ggaagccctc gaacatcagc agcgtccaca atggcgcgct gtagtcgcgc ctgcccgatt
      481 cgtgcgcgct gacaatctct tcgagcacgc gcggctcgaa gaggccggta tcgcgcagcg
      541 tgtcaccgag caccgccgtg cgcacggtct cgcgcagcgg gccgcggaac cagcggacca
      601 gcggcaccgc gaaacccatt ttcgggcgat acagcacgtc gtgcggcagc cacggctcca
      661 tgctcttctt gagcaggcgc ttgccctcgc cgccgcgcat cttgaagctg ctcggcaagg
      721 tcccgagcca ctcgaccagc gggtggtcca tcagcggttc gcgcacttcg agcgaatgcg
      781 ccatgctggc gcgatcgacc ttggtgttga tgtcgcccac caggtaggtc ttcagatcga
      841 ggtactggat cagcgcgagc ggatcgtcgg tgccggcgcg ggcggcatgg cgggcgaaca
      901 ggtcgtcgac ccggtaaccg ccgagcgagg cgcgcatccc tcccgagaac agccgcgcgc
      961 gcatcggcgc gcgcacgatc gacacgctct gcagataggc ttcgaccgag ttgcgtgcca
     1021 gcgattcgaa ggtcgtcttg gcgcggaaga cgcgcggggc ccagtccgcc ttcggataga
     1081 gccgacccag caggccgaac accggacggc gaaagccgag cggcagcgcc gagcgcagtt
     1141 tctcttccat caggtgcagc tggtagcggc gatagccgcc gaagctctcg tcaccgccgt
     1201 cgccggacag cgccacggtc acgtgcttgc gggcgagctc gcacacgcgg taggtgggga
     1261 tcgcggagct gtccgcatag ggctcgtcgt agaggccgac cagggtgtcg atcaggccga
     1321 agtcgtcgct cgccacctgg tcgacgtagt gccgggtctg gtagcgatcg gccaccatct
     1381 gcgcgaactt cgtctcgtcg aatgcggggt cgtcgaagga aatcgaacag gtattgaccg
     1441 gcgcgtcact gatgcgcgcc atcgtcgcta cgaccgcgct cgaatcgacg ccgcccgaga
     1501 ggaatgcgcc gagcggcact tccgagatca tgcgcaggcg gatcgagtcc tgcagccgcc
     1561 ggtcgagctc ggccatcgcg tcctcgaagc cgatgcgatt gtcgagcgag aagcgaacgt
     1621 cccagtactc gcgcggcgcg ggcgccgtct cgcctcgcct gatcacgagc gtgtgcgccg
     1681 gcggaagctt gcgcacgctc gtgtagatcg tgcgcggttc ggccacgtag ccgacggcga
     1741 aatactcttc gaccgcgcac gggtcgatcg tgcgggtcac gcccgggtgc agcaggagtg
     1801 ccttcagctc cgagccgaac acgcactggc catccggcaa cagcgcgtag aacagcggtt
     1861 tcacgccgag ccggtcgcgg gcgagaaaca gggtttcgcg attgcggtcc cacagcgcga
     1921 acgcgaacat gccgcgaaag cgcgtgacgc agtcctcgcc ccaggcctcc caggcgtgga
     1981 cgatgacttc ggtgtcgctc ttggtgtgaa acacatgccc gagcgcctgc agctcgggaa
     2041 tcagcgactg gtagttatag atctcgccgt tgtagaccac gacgacggac ccgtcttcgt
     2101 tgtagagcgg ctgctggccg gtcgcgatat cgatgatcga caggcgccgg tgtccgagcg
     2161 cgacgcccgg ctcgtggtgc tcgccctcac cgtccggtcc gcggtgctgg atcgagtcgt
     2221 tcatccgccg cagcagcgcg cggtcgagct cgcgacggcc cctgagatcg acgattccgg
     2281 tcagtccgca catgggtcag tgatccgtga gaaggccatc gtagaggccg gtgtacgcct
     2341 gcaccatcga gtccatcgcg aacgcgcgca ggcaacgctc gcggccggcc gcaccgtgcg
     2401 ccgcacgcag tccgccgtca tcgagatagc gggcgatcgc cgcagccagt gcggcgggat
     2461 cgcgcggatc gtaaagggtg ccgctgacac cctgctcgat cagttcacca ttcccgccga
     2521 cctgcccggc gacgaccggg cggctcgagg ccatcgcctc gaggatggtg ttggaaatgc
     2581 cctcgttctg cgagggcagc acgaatatgt caaagctgcg cagcagcgcc ggggcatccg
     2641 ggcgcatccc tggcatccac acgagcggcg cgataccgag cgcctgggcc cgctcgcgca
     2701 gccggccggc ctccgggccg tcccccacga tcgcgagcct gagacgaccc tgcggcgcct
     2761 ccggcgcctt ggcaagccgt gcgaaagcct cgagcagcgc cgcctggttc ttgatcgggt
     2821 cgagccgccc gatggtgccg atcacgaggg ggcgctcggc gccctgcacg gcccagggca
     2881 ggtccgcggg caagggtccc tcgggtgaga agcgatcgac gtcgaccccg ttgtagatct
     2941 gccggatccg ccccggcggc accttgatgc gctcctgcag ccagcgctcg atgtccttcg
     3001 agacggccac gtaacgctgc gtcgcggggc ggcacaggcg ccggatgcgc agtgcacgcg
     3061 ggctctcgcc ccgcgggtcc gcggcatccc agccgtgctc gccgtgcacg cggtggcgca
     3121 cgccagccgc cagcgccacc cattgcaggt cgaccgtgcc gagattgcgc gtgtgcacga
     3181 cggccgggcg caggcggcgc aattgccgcc acagtcgccc ataggcggcc gggtccttgc
     3241 ccggctgctt gtcgatcgag atcacctcga catcgtcgcg ctggatgcgc ttgcggaagt
     3301 cggtgaaccc ggcgaggcac acgatggcgt gccggtactt cgcgcgcggc aagcggttga
     3361 cgagattcac gaggccgttc tcgagcccac cgtagtcgag ccggtaaatg acatgcgcga
     3421 tgaggggagc ctcggccatg cgcctaccag cagatgcccg agtagcgccc cgctctgccg
     3481 ccgcgcatcg caatgcccac cacgtcgacg accgcatgac gcggttcgag cgtcgccgcg
     3541 agtgccgctt cgatgtcctt gccaccgagg ccgaccagga tcgtgtcgcc gtggctcacc
     3601 gcctcctcgc aggagccgac catcagcttg ccgatgtgcg ggatgctctg ctcgatgaag
     3661 cgcttgttgg caccgatcag gcgcgacagg ttcacttccg ggtcgtagat gcgcaggtca
     3721 tagcccttgc cgatcagccg ctcggccacg gagaccagcg ggctttcgcg caggtcgtcg
     3781 gtgcccttct tgaaactgag gccgatcatg ccgaccttgc gcgtctcgcc ccgcatcacc
     3841 aggtcgatcg cctggtcgat gtgcgcgcgg ttgctgccga gcacgccgcc gagcatcggc
     3901 gtggtgacgt cgtgcgtctt gcccatgtag gtcagcgcgc gcaggtcctt cggcaggcag
     3961 gagccgccga acgcgaatcc cggccgcagg taggcgggcg agatgttgag ctgcgtatct
     4021 ttgcagatga gcgccatcac ctcgcgggca tcgacatcga ccgcgctggc gaggcgaccg
     4081 atttcgttcg cgaaagtgat cttgaccgcg tggaaggcat tcgcggcgta cttctgcatc
     4141 tcggcgacgc cgaccgaggt gacgacgaac tcgcacggca gatgtccgaa caccgcgcgc
     4201 agcgcgtcgg cggcgcgtgt cgagcgcgtg ccgacgatcg tgatcggcgg gcggtcgtag
     4261 tccttgatcg aggtgccctc gcgcaggaac tcgggctgga aggcgagacc gaagtccctg
     4321 tcggccacct tgccgctcgc tttctccagc gtcgggcgca ccagttcatc gagcgtgccg
     4381 ggctgcacgg tcgagcgcag cacgacagta tggaatgccg acttcttcgc cagcgcctca
     4441 ccgatcgact ccatgacgcg ccggatgtag ctcatgtcct ggctgccgtt cgggctcgag
     4501 ggcgtgccga cgcagacgaa ggacagctcg gtggcctgga tcgcggcggc cacgtcgtcg
     4561 gtgaccttca ccttgcccga cgcgacgaca tcgcgcgtca ggtcctggat gccctcctcg
     4621 acgatcggcg acttgccgct ccggatcagc tcgagtttga gcggatcgac atcgacgccg
     4681 gtcacctcgt ggccatccct cgcgagacag gccatcgaga ccgcaccgac atagcccagg
     4741 ccgaaaatac tgatcttcat ttacctgctt cctcgatcag gccatcgagc cggcgcatcg
     4801 atgccgccca gctgtgatgg ctcaacacac gatcgcgacc ggcggccgcc aagcgccggc
     4861 gcgcgtccgc gtctcccaac aaacgatcga tggcggcgac ccattcgtcg gtgcccgatg
     4921 ccacgagcag gtgttcaccc gccaccgcat cgacaccgcc cgaagcctgc ggactgcaga
     4981 ccaccgggac ccccatcgcc atcgactcca ggatcttgtt ctgcgtgccc cgcgcgatcg
     5041 cgagcggcgc gatcgtgagc gcagcacggc gcacatgcgg gcgcacgtcg ggcaccgacc
     5101 cagtgaccgt gacgcccggc aattttccca gcgcgcggat gtgctccggc ggatccgccc
     5161 cgacgatcgc aaaccgcgcg gccggtcgct tcgcctgcag cgccggcagc acgtcgcgac
     5221 agaagccgag cacgccctgc tggttcgggt agtagtccat gcgcccgacg aaggcgagga
     5281 gatcggtgtc gtagccctcc ccgtcgggtg cgaagaaccc ggcatccacg ccgttcggga
     5341 accagtccga gggcgtcgac acgccgagcg cccgcagcga ctcgagctcg gcgcgtgtcg
     5401 tgcaggtgca caggtcgaag tgatcggcga gctggcgctc gcggcgttcg agctttaccg
     5461 cttcgagcca gtagcccatc gagagcggga aggcacgatg acccgagtac tcgcgccact
     5521 tctgcgagtc catgtcgccg tagtcgagga ccttgagcgg cgccgcgaga cgctccacat
     5581 agggcgcgac cgacgagcaa tgcacgaata cgagattgaa acgcgtggcc ttcgcggccg
     5641 cctcgatccg ccgcgccagc ctgcgcgacc agaaatagcc gaagctcgac gggctcgcgg
     5701 tcggcagacg cgccaccatc tgcggccagg cgacgcgatc cgggatcacc tcgacgatgg
     5761 cctcggcgca gtgctgcgca aggccccgcg actcctcgag ctcggcggca ctgcgtgcca
     5821 gcgaggccac ggtcacctca tggcccgcgt cggtcaggtg ccggatgatg ttgaagggcc
     5881 ggatcttgcc gccgcgcttc ggcggaaacg gcacgcgatg gcacacgaac aggaccttca
     5941 tgcggcgagc ttcgctttca ggtcctccgt gacaccggcc acgctggcgc catcgaggta
     6001 caggcgcgcc cagacctcga gattgacgag tgccagcagg tgatcggtgc gatcggcctg
     6061 ctgctgttcg tgttcggcga tggtgcgcgc taccgcatcg gggtcgagca ggccgcgccg
     6121 caggatcgtc tcgcgcgaca gcaggtcggc gaccagcggg gcgagttcct gcttgaacca
     6181 ggcgccgatc ggcgcgccga agccgcgctt ttcgcgattg aggatgctgt ccggcaggat
     6241 gccggcgagc gcacgcttca tcgcgtactt gaggtcgcca ccgcggacct tgagcgcccc
     6301 cggcatgcgc gcggcgaact ccaccaggcg ctggtcgagc agcggcacgc ggcactcgag
     6361 cgagaccgcc atgctcatct tgtcggtgag catcagcagg tcttcgggca tctgcgtggc
     6421 gagatccacg tccatcagcc gtcgcagcgg atcggtggca ttcgacgccg cgaacgcacg
     6481 ggcgatgcag tcgtcgaagg ccggcggctc gccgagcagc agtgccgccc gctcgtccgc
     6541 gctgaacact tccatgtagc gccgatagcg ctcctcgaac gacagcgagt cggccagcac
     6601 gaacgccttg gcgagtcgca gttcgttgag caaccggtga tgccggtcgc tcggcagccg
     6661 ccgcgccagc ggatcgatca tcgagcggcg aatgaagcgc gggatgcgct ggtactggcg
     6721 gcggtagtgc tcgtcgagat agcggcgata gccgccgaac agttcgtcgc caccgacgcc
     6781 ggagaggatc acggtgacgt cttcgcgggc gaacttcgac acgagatagg tggtgatgaa
     6841 cgccgcatcg gccaccggct cgtccatgtg cgacagcagg cccggcagca gcgccgccac
     6901 gtccggctgt acgaggattt ccttgtgctc ggtaccgaag gcccgggcca cggcgcgcgc
     6961 gtgcggcagt tcgttgtaga gcgcggcgcc cgaggagccc gcgaagccga tcgaataagt
     7021 gcgcacgggc cgatcggtgt gccgcgacat gaaagcgacg accgcgctcg aatcgatgcc
     7081 gccggagagg aacgccccga tcggcacgtc gctgaccatc tgctcgcgta ccgagcgctc
     7141 gagctcgctg cgcagcccgt cgatccacga ctgctcgctg cgcgctgcat cgatgcccgc
     7201 gtcaaggcgc cagtactggt tcaccgccac gcggccctcc tcggccacca gca
//
LOCUS       DAOQVY010000122         7060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_11746_length_7060_cov_6.271806, whole genome shotgun sequence.
ACCESSION   DAOQVY010000122 DAOQVY010000000
VERSION     DAOQVY010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11746_length_7060_cov_6.271806"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="PKN91_10460"
     CDS             <1..862
                     /locus_tag="PKN91_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNS28531.1"
                     /translation="FWFFGHPEVYIMILPAFGVVSEIIPAFSRKPLFGYESMVYATAA
                     IGFLSFIVWAHHMFTVGMPLAGQLFFMLATMLISVPTGVKVFNWCATMTRGSLTFEPP
                     MLFAIAFLFLFTIGGFSGLMLAIVPADFQYHDTYFVVAHFHYVLVPGSLFAIMGAVYY
                     WLPKWVGRMYDMKLAKIHFWLSAIFVNVLFFPQHFLGLAGMPRRIPDYATQFADWNMV
                     SSIGAFGFGLSQLLLPYIIIKCVRSGEKATDRVWDGARGLEWELPSPAPFHSWETPPS
                     AAVIARGAEH"
     gene            859..996
                     /locus_tag="PKN91_10465"
     CDS             859..996
                     /locus_tag="PKN91_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28532.1"
                     /translation="MSRELDYGAADETRRRVKRSAIVLGLVAFAVYVGFIVMSIARGM
                     P"
     gene            993..1550
                     /locus_tag="PKN91_10470"
     CDS             993..1550
                     /locus_tag="PKN91_10470"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HNS28533.1"
                     /translation="MSDAAGQRARDNARLTARLALMTLGSFAFGFALVPLYDVMCTIT
                     GFGNEKNLARASDVAPQAADESRTVTVEFIAELPNVGSWEFTPAVTSMKVHPGKLYET
                     SFVAKNLTGNDTTMQAVPNLAPRNAAGYFHKTECFCFTPQQFSKDEQRSMPVRFIVDR
                     ALPQHLDRVTLAYTIYDQSIRVGLK"
     gene            1553..2440
                     /locus_tag="PKN91_10475"
     CDS             1553..2440
                     /locus_tag="PKN91_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNS28534.1"
                     /translation="MATPSAHTADKYFVPHGSRWPITASAALFVMMFGAVAYLNEWFG
                     NWIFIPGALLLAYMFFGWFHDVIGESQSGAYNAQVDRSFRMGMMWFIFSEVMFFAAFF
                     GALFYARSLAIPWIGGEGVKVFNKLLLYQDFDPAWPTNGPARVGGHEDGSFQIIPAFG
                     LPAINTAILLTSGVTITIAHHALQAGKRTQLKIFLAATFLLGFLFVGLQAEEYIHAYQ
                     ELGLRLSTGIYGSTFFMLTGFHGAHVTIGAIMLLIIWLRVMKGHFTPKSHFAFEGVAW
                     YWHFVDVVWLGLFIFVYWI"
     gene            complement(2451..3356)
                     /locus_tag="PKN91_10480"
     CDS             complement(2451..3356)
                     /locus_tag="PKN91_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNS28535.1"
                     /translation="MHKILVVVDPTASRWPSVEKAARIARGVGAALELYVCDVDQDVP
                     EGRSDASGIADHRARLRARRLETLERLAVPLREAGLTVTTAAEWHAPLEEGIGHHVIR
                     TAPDLVVKDTHWRAAVPRAMLTRTDTNLIRQIPAPLLLVRETEWPRRPLVAVAVDPCH
                     PADYPPALDQAMAGIGRRLGDALDGHVELLHVLQDPPHLPGDKVGVLERAQAHAKARA
                     AVERLAGDSGLAVHYTEGRGLDGLVSLAARREPTVLVMGVASRPRWQHSAGGGTAATV
                     LERAECDLLAVKPPGFVSPLLVTDD"
     gene            3445..4491
                     /gene="ptcA"
                     /locus_tag="PKN91_10485"
     CDS             3445..4491
                     /gene="ptcA"
                     /locus_tag="PKN91_10485"
                     /EC_number="2.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR04384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050231 - putrescine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009445 - putrescine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine carbamoyltransferase"
                     /protein_id="HNS28536.1"
                     /translation="MKPVRHFVDTQDFDKAELLTIMELIASLKEADKAGACPPLLKGA
                     SLGMLFEEPSTRTRVSFEVAMTRLGGHALYLRPGEIHLGARESLNDTADVLERMVDVI
                     EARTLKHETVVGLAAKADVPVLNGLTDYNHPTQVLCDVFTMSEYLPPGKRLEELQVTF
                     IGDATNVCNSLAAICTQMGMHFTHAAPARYQIAEKVRAIATANCAVSGGTLRLTDDPV
                     AAVAHADFVYTDLWWWVGQEAEIPDRTRAFMPRYQLNKALLAKAPAHCRVMHCLPASR
                     GVEATDEVLDSPQSIIFDQSENRLHTEKGLLVWFVYPHLKRPSPELRAYHAGRIEHFL
                     DRRLEAHVPGLPKR"
     gene            4503..5915
                     /locus_tag="PKN91_10490"
     CDS             4503..5915
                     /locus_tag="PKN91_10490"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNS28537.1"
                     /translation="MTAPSTGRGFHRVLRRLDVTLFTVCAILVIDQLAASASIGASSI
                     FWWVFTLVFFFVPYALISAELGATYPEEGGIYAWVRRAYGPRWGARTSWFYWINVALW
                     MPSVYVLFAGIFAQMFAPDLSLWAQIAIGIVLTWVTVYINVISLAVGKWVPNGGAVIK
                     AAIMVTIGVGGIVYATRHGVANDMSLRAMAPSWGAGLAFLPVIVYNFLGFELMSGASE
                     EMKSPQRDVPIAIGIAGLLISTFYLLGTVGILIALPVDEIGLIEGLLDSLHKLFGEYS
                     WGPAFVTVLGIGALATFIANMVTWTMGANRSASEAAERGDLPRVFGKLHPIHRTPASA
                     AIITGVVSTVVIIIYGLFAATAEDLFWSLFAFSSIVFLMPYILMFAAFARLRVTEASH
                     ARPYRMPGGNMAAKLAATVCILFILQAIVLFVWVPGEPLDTSQAVLLVVGVIVTWIVG
                     DVLISRAQRAAPPRGGATGS"
     gene            5912..7018
                     /gene="aguA"
                     /locus_tag="PKN91_10495"
     CDS             5912..7018
                     /gene="aguA"
                     /locus_tag="PKN91_10495"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: protein motif:HMM:NF010070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HNS28538.1"
                     /translation="MTRVIESTPRRDGFRMPGEHEPHAGTWMLWPERPDNWRNGAKPA
                     QHAFVAVASAIAQFEPVTMGVNQRQFDNARASLPSQVRVVEISNNDSWMRDCGPTFVV
                     NGQGQVRGIDWQFNAWGGLAGGLYFPWDLDDLVPVKMLEIEGIDRYDAPLVMEGGSIH
                     VDGDGTLFTTEECLLNPNRNPGLSKAEIERLLGDYLDVAKVVWIDRGIFNDETSGHID
                     NICAPVRPGVVALAWTDDRSDPQYEISQDAYQRLSAATDARGRKLEVVRIHIPDPVLI
                     SREEAAGVDSVRGTLPRKAGDRLSASYVNYYVCNGGVVVPTFGDPHDQAALDLLARLY
                     PDRRVVGVPAREILLGGGNIHCITQQQPAARPRA"
BASE COUNT         1101 a         2445 c         2262 g         1252 t
ORIGIN      
        1 cttctggttc ttcgggcatc ccgaggtcta catcatgatc ctgccggcgt tcggcgtcgt
       61 ctcggagatc atccctgcct tctcgcgcaa gccgctgttc ggctacgagt cgatggtgta
      121 cgcgaccgcg gcgatcggct tcctgtcgtt catcgtgtgg gcgcaccata tgttcacggt
      181 cggcatgccg ctcgccggcc agctgttctt catgctcgcg accatgctga tctcggtgcc
      241 caccggcgtg aaggtgttca actggtgcgc gacgatgacc cgcggctcgc tcactttcga
      301 gccgccgatg ctgttcgcga tcgcgttcct gttcctgttc acgatcggcg gattttcggg
      361 cctgatgctc gcgatcgtgc cggccgactt ccagtaccac gacacctact tcgtagtcgc
      421 gcacttccac tacgtgctgg tgcccggctc gctgttcgcg atcatgggtg cggtgtacta
      481 ctggctgccg aagtgggtgg gcaggatgta cgacatgaag ctcgccaaga tccacttctg
      541 gctgtcggcc atcttcgtga acgtgctgtt cttcccgcag cacttcctcg ggctcgccgg
      601 catgccgcgg cgtatccccg actatgcgac gcagttcgcc gactggaaca tggtttcctc
      661 gatcggcgcg ttcggcttcg gcctctcgca gctgctgctg ccgtacatca tcatcaagtg
      721 cgtgcgttcg ggcgagaagg ccacggaccg cgtttgggac ggcgctcgcg gcctcgagtg
      781 ggagctgccc tcgccggcgc cgttccacag ctgggagacg ccgccgtcgg ccgccgtgat
      841 cgcgcgcggg gccgagcatt gagccgcgag ctcgactacg gcgcggcgga tgagaccagg
      901 cgccgcgtga agcgcagcgc gatagtgctg ggcctggtcg cgttcgcggt ctatgtcggc
      961 ttcatcgtga tgtcgatcgc acggggcatg ccgtgagcga cgccgccggg cagcgcgcgc
     1021 gcgacaacgc ccggctcacc gcccggctcg cgctgatgac cctgggcagc ttcgcgttcg
     1081 gcttcgcgct cgtgccgctc tatgacgtga tgtgcacgat caccggcttc ggcaacgaga
     1141 agaacctggc gcgcgcgtcc gacgtcgcgc cgcaggcggc tgacgagagc cgcaccgtca
     1201 ccgtggagtt catcgccgaa ctgccgaacg tcggcagctg ggagttcacg ccggccgtca
     1261 cctcgatgaa ggtgcatccc ggcaagctct acgagacctc cttcgtcgcg aagaacctca
     1321 ctggcaacga caccacgatg caggcggtgc cgaacctcgc gccgcgcaat gccgcgggct
     1381 acttccacaa gaccgagtgc ttctgcttca cgccgcagca gttctccaag gatgagcagc
     1441 gcagcatgcc ggtgcgcttc atcgtcgatc gggcgctgcc ccagcatctc gatcgcgtca
     1501 cgctcgccta caccatctac gatcaatcga ttcgcgtagg gctcaaataa ccatggcaac
     1561 tccatcagcg cataccgccg acaaatactt cgtcccccac ggcagccggt ggccgatcac
     1621 cgcctcggca gcgctgttcg tcatgatgtt cggggcggtc gcgtacctga acgagtggtt
     1681 cggcaactgg atcttcatcc ccggcgcgct gctgctcgcc tacatgttct tcggctggtt
     1741 ccacgacgtg atcggcgaga gccagagtgg cgcctacaac gcgcaggtcg accgctcgtt
     1801 ccgcatggga atgatgtggt tcatcttctc cgaggtgatg tttttcgccg cgttcttcgg
     1861 cgcgctgttc tacgcgcgtt cgctcgcgat accgtggatc ggcggcgagg gcgtcaaggt
     1921 gttcaacaag ctgctgctgt accaggactt cgatccggcc tggccgacca acggcccggc
     1981 gcgcgtcggt ggccacgaag acggctcgtt ccagatcatc ccggcgttcg gcctgccggc
     2041 gatcaacacc gcgatcctgc tgacctcggg cgtcacgatc acgatcgcgc accacgcgct
     2101 gcaggcgggc aagcgcacgc agctgaagat cttcctcgcc gcgaccttcc tgctcggctt
     2161 cctgttcgtc ggcctgcagg ccgaggaata tatccacgcc taccaggagc tcggcctcag
     2221 gctctcgacc ggcatctacg gctcgacctt cttcatgctg accgggttcc acggcgcgca
     2281 cgtgacgatc ggtgcgatca tgctgctgat catctggctg cgcgtcatga agggtcactt
     2341 cacgccgaag agccacttcg ccttcgaagg ggtggcctgg tactggcact tcgtcgacgt
     2401 ggtctggctc gggctgttca tcttcgtcta ctggatctga tcgcgcgaga tcagtcatcc
     2461 gtgacgagca gcgggctgac gaagccgggc ggcttgacgg ccagcaggtc gcattccgcg
     2521 cgttcgagca ccgtggccgc cgtcccgccg ccggccgaat gctgccagcg gggccgcgac
     2581 gccaccccca tgacgagcac cgtcggctcg cgcctcgctg ccaggctgac gagcccgtcg
     2641 aggccgcggc cttccgtgta gtgcaccgcg agccctgaat ctccggcgag ccgctccacg
     2701 gccgcccgcg ccttcgcgtg cgcctgcgcc cgctcgagca ccccgacctt gtcacccggc
     2761 aggtgcggcg ggtcctgcaa cacgtgcagc agttccacat ggccatcgag ggcatcgccg
     2821 agccggcgac cgattcccgc catcgcctgg tcgagcgccg gggggtagtc ggcgggatgg
     2881 cagggatcta cggcgactgc gaccagcggc cgccgaggcc attccgtctc gcgcacgagc
     2941 aataaggggg cgggaatctg ccgaatcagg ttcgtgtcgg tacgcgtgag cattgcccgc
     3001 ggcacggcgg cgcgccagtg cgtgtccttg acgacgagat cgggcgccgt acggatgacg
     3061 tgatgaccga tgccctcttc gagcggcgca tgccactcgg cggcggtcgt gaccgtgagg
     3121 ccggcctccc gtagcggtac cgcgaggcgc tcgagggtct cgaggcgacg cgccctgagt
     3181 cgcgcgcgat ggtccgcgat gcccgacgcg tcgctccgac cttcgggcac gtcctggtcc
     3241 acgtcgcaca catacagttc gagcgcagcg ccgacgccgc gcgcgatgcg cgccgccttc
     3301 tccacgctcg gccagcggct cgcggtggga tcaaccacca ccagtatctt gtgcatcagg
     3361 cgctccatag gcacttcggc gcagcgggcg gcccggcaag acccaggatg gcgctgcggg
     3421 gaacttgatc ttaagcttgc cagcatgaag cctgtacgcc atttcgtcga cactcaggac
     3481 ttcgacaagg cggagctgct gacgatcatg gaactgatcg cctcgctcaa ggaggccgac
     3541 aaggcaggcg cctgcccgcc gctgctgaag ggcgcctcgc tcggcatgct gttcgaggaa
     3601 ccctccaccc gcacgcgcgt ctcgttcgaa gtcgcgatga cgcgcctcgg cggacacgcg
     3661 ctgtacctga gacccggcga gatccacctc ggcgcgcgcg aatcgctcaa cgacacggcg
     3721 gacgtgctcg aacgcatggt cgacgtgatc gaggcgcgca ccctcaagca cgaaaccgtc
     3781 gtcgggctcg ccgccaaggc cgacgtgccg gtgctgaacg gcctgaccga ctacaaccac
     3841 ccgacccagg tgctgtgcga cgtcttcacg atgagcgagt acctgccgcc tggcaaacgg
     3901 ctcgaggagc tgcaggtcac gttcatcggc gatgcaacca acgtctgcaa ttcgctcgcg
     3961 gcgatctgca cccagatggg catgcacttc acgcacgcgg cgcccgcccg ctaccagata
     4021 gcggagaagg tgcgcgcgat cgcgacggcc aattgtgcgg tctcgggcgg caccttgcgg
     4081 ctcaccgacg acccggtcgc ggccgtcgcc catgcggact tcgtctacac ggatctctgg
     4141 tggtgggtgg ggcaggaagc ggagattccc gaccgcacca gggcgttcat gccgcgctac
     4201 cagctcaaca aggcactgct cgccaaggcg ccggcgcact gtcgcgtgat gcactgcctg
     4261 cccgcctcgc gcggcgtcga agcgaccgac gaagtgctcg actcgccgca atcgatcatt
     4321 ttcgaccagt ccgagaatcg cctgcatacc gagaagggcc tgctggtgtg gttcgtctac
     4381 ccgcacctca agcgccccag ccccgaactt cgcgcctacc acgcaggccg gatcgagcat
     4441 ttcctcgatc gccgcctcga ggcgcatgtg ccgggcctac ccaagagata ggggaaacca
     4501 tcttgacagc accaagcacg ggccgcggat tccatcgagt tctgcgcagg ctcgacgtga
     4561 ccctgttcac cgtctgcgcg atcctcgtga tcgatcagct ggccgcatcg gcctcgatcg
     4621 gcgcctccag catcttctgg tgggtgttca ccctcgtctt cttctttgtg ccctacgcgc
     4681 tgatctcggc cgaactcggc gcgacctatc cggaggaagg cggcatctat gcctgggtgc
     4741 gccgcgccta tggcccgcgc tggggtgcac gcacctcgtg gttctactgg atcaacgtcg
     4801 cgctgtggat gccctcggtg tacgtgctgt tcgccggtat cttcgcgcag atgttcgcgc
     4861 cggacctgtc gctgtgggca cagatcgcga tcggcatcgt gctgacctgg gtcaccgtat
     4921 acatcaacgt gatctcgctc gctgtcggca agtgggtacc gaacggcggc gccgtcatca
     4981 aggccgcgat catggtcacg atcggcgtcg gcggcatcgt ctatgcaacg cgccacggcg
     5041 tcgccaacga catgtcgctg cgcgcgatgg cgcccagctg gggcgccgga ctcgcgttcc
     5101 tgccggtcat cgtctacaac ttcctcggtt tcgagctgat gtccggcgcc agcgaggaga
     5161 tgaagagccc gcaacgcgac gtgccgatcg cgatcggaat cgcggggctg ctgatctcca
     5221 cgttctacct gctcgggaca gtcggcatcc tgatcgcact gccggtggac gagatcggcc
     5281 tgatcgaagg cctgctcgac tcgctgcaca agctgttcgg cgagtacagc tggggccctg
     5341 ccttcgtgac cgtgctcggc atcggcgcac tcgccacctt catcgccaac atggtgacct
     5401 ggacgatggg cgcgaaccgc tcggcaagcg aggcggccga gcgaggcgac ctgccgcgcg
     5461 tgttcggcaa gctgcacccg atacatcgca cgcctgcgag cgccgcgatc atcaccggcg
     5521 tcgtgtcgac cgtcgtgatc atcatctatg gcctgttcgc cgccaccgcc gaagacctgt
     5581 tctggtcgct gttcgcattc agctcgatcg tgttcctgat gccttacatc ctgatgttcg
     5641 ccgcgttcgc caggctgcgg gtcaccgagg cctcgcatgc ccggccctat cgcatgccgg
     5701 gcggcaacat ggcggccaag ctcgccgcga ccgtgtgcat cctgttcatc ctgcaggcga
     5761 tcgtgctgtt cgtctgggtg ccgggcgagc cgctcgacac gtcgcaggcg gtgctgctgg
     5821 tggtcggggt catcgtcacc tggatcgtcg gcgatgtgct catctcccgc gcgcagcgcg
     5881 ctgccccgcc ccgcggcgga gcgaccgggt cgtgacgcgc gtcatcgagt ccacgccacg
     5941 gcgcgacggc tttcgcatgc ccggcgagca cgagccgcat gccggcacct ggatgctctg
     6001 gcccgagcga ccggacaact ggcgcaacgg cgccaagccc gcgcagcacg ccttcgtcgc
     6061 cgtcgccagc gcgatcgcgc agttcgaacc ggtgacgatg ggcgtcaacc agcgccagtt
     6121 cgacaacgcg cgcgccagcc tgccgtcgca ggtgcgggtc gtcgaaatat cgaacaacga
     6181 ctcgtggatg cgtgactgcg gacccacctt cgtcgtgaac ggacagggcc aggtgcgcgg
     6241 catcgactgg cagttcaatg cctggggcgg gctcgccggc gggctgtact tcccctggga
     6301 cctcgacgac ctcgtgccgg tgaagatgct cgagatcgag ggcatcgatc ggtacgacgc
     6361 cccgctcgtc atggaaggcg gctccatcca cgtggacgga gacggcacgc tgttcacgac
     6421 cgaggaatgc ctgctgaacc ccaatcgcaa cccggggctc tccaaggccg agatcgagcg
     6481 cctgctcggc gactacctgg acgtggcgaa ggtcgtgtgg atcgaccggg gcatcttcaa
     6541 cgacgagacg agcggccaca tcgacaacat ctgcgcgccg gtccggccgg gcgtcgtggc
     6601 gctcgcgtgg accgacgaca ggagcgaccc gcagtacgag atctcgcagg atgcctacca
     6661 gcgcctgtcc gcggccaccg atgcgcgcgg ccgcaagctc gaagtggtgc gcatccacat
     6721 acccgacccg gtgctgattt cgcgcgagga ggcagcgggt gtcgacagcg tgcgcggaac
     6781 cctgccgcgc aaggccggcg atcgcctctc ggcctcctac gtcaactact acgtctgcaa
     6841 tggcggcgtc gtcgtgccga ccttcggcga cccgcacgac caggcggcgc tcgatctgct
     6901 cgccaggctg taccccgacc ggcgcgtggt cggagtgccc gcgcgagaaa tcctgctcgg
     6961 cggcggcaac atccactgca tcacgcagca gcagcccgcc gcgcgcccgc gcgcctgacg
     7021 cgtgcgcatc gtcatcgcac tcggtggcaa cgcgctcctg
//
LOCUS       DAOQVY010000123         7000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_11880_length_7000_cov_5.877898, whole genome shotgun sequence.
ACCESSION   DAOQVY010000123 DAOQVY010000000
VERSION     DAOQVY010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11880_length_7000_cov_5.877898"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /gene="groL"
                     /locus_tag="PKN91_10500"
     CDS             complement(<1..1215)
                     /gene="groL"
                     /locus_tag="PKN91_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNS28539.1"
                     /translation="MAAKEVRFSDDARTKMFRGVNVLANAVKATLGPKGRNAVLEKSF
                     GAPTITKDGVSVAKEIELKDKFENMGAQMVKEVASNTSDEAGDGTTTATVLAQAIIRE
                     GLKAVASGRNPMDVKRGIDKAVLAATEELKKLSKPCKDSKAIAQVGTISANSDESIGK
                     TIADAMEKVGKEGVITVEEGSGLANELDVVEGMQFDRGYLSPYFINSQANQTAELEKP
                     LILLVDKKISNIREMLPVLEAVAKAGRPLLVIAEDVEGEALATLVVNNIRGILKVAAV
                     KAPGFGDRRKAMLQDIAVLTGGTVISDEVGLQLEKATLNDLGEAKKVVVEKENTTVID
                     GAGKASDIKARIESIRQQAEEATSDYDKEKLQERVAKLSGGVAVIKVGAATEIEMKEK
                     KARVEDALHATRA"
     gene            complement(1217..1510)
                     /gene="groES"
                     /locus_tag="PKN91_10505"
     CDS             complement(1217..1510)
                     /gene="groES"
                     /locus_tag="PKN91_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009684394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNS28540.1"
                     /translation="MKIRPLHDRVIVKRLEEERTSPGGIVIPDSAAEKPIQGKVVAIG
                     KGKILEDGKVRPLDIKVGDKILFGKYSGTEVKVDGEDLLVMREEDVMAVIEGK"
     gene            1726..3930
                     /gene="dsbD"
                     /locus_tag="PKN91_10510"
     CDS             1726..3930
                     /gene="dsbD"
                     /locus_tag="PKN91_10510"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HNS28541.1"
                     /translation="MTNRFPRARWLALALLLSSPAASVLADEFLPPEQAFRYSLDSDG
                     QKIVVHWDVEPGYYLYLKRMGVSSTAEGLTLGEAVYPQGEVHTDEYFGEQVVFRGRFD
                     VTVPITVAPGTSARDIPVTLKLQGCADAGLCYPPTAWDTSVTIPAAGDATVPAATGLD
                     ALMAQARSTGGFGAGGDEFLDPDVAFRLGAVADGPDRVRLTWEIAAQYYLYREKLGVK
                     TGSAASVGQLVLPAGEVHSDEYFGEQEVYREELVATIPVARAGGTAMTIALDVTYQGC
                     ADAGLCYPPITKTLNVDLPAGGTMSAASGGFVSKQDKMAAFIRSGNLLFVIAFFFGAG
                     LLLAFTPCVLPMVPILSGIIAGQGANVTTRRAFFLSLSYVLGMAFTYTLAGIIAAAAG
                     AQVQAVFQQPWIVTVFALLFVVLALSMFGLFTIQMPAAIQTRLADASNRQRAGTFGGV
                     AAMGALSALIVTTCVAPALVATLAVIGQSGNVVRGGLALFAMSLGMGAPLLAVGASAG
                     KLLPKAGPWMDTVKQLFGVLFLLVAAWMLARLVPGPLALLLWAVPALAGAWVCFKAAK
                     QARKGRPWWRSAGAVTGLYGVVLLAGAALGGGDPLAPIPQLAAKHEELGFRRIASLEE
                     LEREVAAAHAAARPVMLDFYADWCVSCKEMEKYTFTDPAVRDALAAAVLLQVDVTANN
                     EQDQALLKHFGIYGPPTIAFYGPDGIERRNYRVVGYMKAAEFSALAREATAP"
     gene            complement(3938..4735)
                     /locus_tag="PKN91_10515"
     CDS             complement(3938..4735)
                     /locus_tag="PKN91_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HNS28542.1"
                     /translation="MSPPIRRLAAAMGVALMLAACGRTQLDLPATLPAEPVRVTASAN
                     GFEGEITIDGGIVAVPRLHELIEPRMRGLLAETQVMSQINAEARGAASAEGLFFKAEW
                     TSSPAGRRYLNVRGLISQFAGGAHPLTVYDAFVWDRLGQRRLALADLLRDPRPGSEAV
                     TALAAAARDALIEVKRSRIPDYAPEADSFIAAEGDGPFVPDLLRFAQNFQLVPADDLG
                     EGGGIELLFSPYDVGPYAEGRFEVLVPGAAVASFLKADAARLLAPLD"
     gene            4787..5482
                     /locus_tag="PKN91_10520"
     CDS             4787..5482
                     /locus_tag="PKN91_10520"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNS28543.1"
                     /translation="MNGMQRFLALAAIMLLAGLAGYFGYRATGDSAAPVTGVAQPATT
                     SPAAGEDAALPTRPAREIPERLPEFTLKDRDGVPRTLAHWAGRPLLVNFWATWCAPCR
                     REIPLLTSLRAEHADEGLEVIGIAVDFREDVLKYAEEIGLEYPLLIGEQDGLEAAAAF
                     GVEPLFPFSVFSDRQGRIVAVRIGELHADEAAFILDRVREVGANRLELPVAQQQIADK
                     LRELAVLRAQSPS"
     gene            5554..6006
                     /gene="aroQ"
                     /locus_tag="PKN91_10525"
     CDS             5554..6006
                     /gene="aroQ"
                     /locus_tag="PKN91_10525"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNS28544.1"
                     /translation="MASLLLLNGPNLNLLGQREPETYGSATLEQLVARAAATARSLGH
                     ELVAFQSNAEHELVDRVQAARSDGTAFIILNPGAFSHTSIALRDALLAVALPFIEVHL
                     SNVFAREAFRHHSYFTDIAVGAILGLGGAGYELAIRAAAEQLAARGGQ"
     gene            6028..6480
                     /gene="accB"
                     /locus_tag="PKN91_10530"
     CDS             6028..6480
                     /gene="accB"
                     /locus_tag="PKN91_10530"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNS28545.1"
                     /translation="MDIRKVKKLIELLEESGIAEIEIREGEESVRISRGSTAPAQQVY
                     AAAPYPAPAPQAAAAPAPAPAASPPPKSNEYVVTAPMVGTFYASPSPGAKPFVDIGDE
                     VKVGQVLCIIEAMKMMNQIESDKAGRITSVMVQNGEPVEFGQPLYTIE"
     gene            6484..>7000
                     /locus_tag="PKN91_10535"
     CDS             6484..>7000
                     /locus_tag="PKN91_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNS28546.1"
                     /translation="MLDKVLIANRGEIALRILRACRELDIKTVAVHSTADANLKHVLL
                     ADESVCIGPPPSPQSYLNMPAIISAAEVTDAVAIHPGYGFLSENADFAERVEKSGFTF
                     IGPKSEVIRLMGDKVSAIKIMKSHGVPCVPGSDGPLGRDNDENLRIARSIGYPVIIKA
                     SGGGGGRGMRVV"
BASE COUNT         1056 a         2431 c         2308 g         1205 t
ORIGIN      
        1 cgcacgcgtg gcgtgcaggg cgtcctcgac gcgggccttc ttttccttca tctcgatttc
       61 ggtggccgca ccgaccttga tcacggcaac accgccggag agcttcgcga cgcgctcctg
      121 cagcttttcc ttgtcgtagt cgctggtcgc ctcttcggcc tgctggcgga tcgactcgat
      181 gcgcgccttg atgtccgacg ccttgccggc gccgtcgatc acggtggtgt tttccttctc
      241 gacgacgacc ttcttggcct caccgagatc gttcagcgtg gccttctcga gctgcaggcc
      301 gacctcgtcc gagatcaccg tgccgcccgt caggacagcg atgtcctgca gcatggcctt
      361 gcggcgatcg ccgaagcccg gggccttgac ggccgcgacc ttgaggatcc cgcggatgtt
      421 gttgacgacg agcgtggcga gcgcctcgcc ctcgacgtcc tcggcgatca cgagcagcgg
      481 gcgacccgcc ttcgcgacgg cctcgagcac cggcagcatt tcgcggatgt tcgagatctt
      541 cttgtcgacc agcaggatca gcggcttttc gagctccgcg gtctggttcg cctggctgtt
      601 gatgaaatac ggcgaaaggt agccgcggtc gaactgcatg ccctcgacga cgtcgagttc
      661 gttcgcgagg cccgagccct cctcgaccgt gatcacgcct tccttgccga ccttctccat
      721 cgcgtcggca atcgtcttgc cgatcgactc gtcggagttc gccgagatcg tgccgacctg
      781 cgcgatcgcc ttcgagtcct tgcagggctt ggagagcttc ttcagctcct cggtggcggc
      841 gagcaccgcc ttgtcgatgc cgcgcttgac gtccatcggg ttgcggcccg aggccacggc
      901 cttcaggccc tcgcggatga tcgcctgcgc gagcaccgtg gcggtcgtcg tgccgtcacc
      961 ggcctcgtcg gaggtgttgg aagcgacttc cttcaccatc tgcgcgccca tgttctcgaa
     1021 cttgtccttg agctcgattt ccttcgcgac cgacacaccg tccttcgtga tggtcggagc
     1081 accgaagctc ttctcgagca ccgcgttgcg gcccttgggg ccgagtgtcg ccttgacggc
     1141 gttggcgagg acattcacgc cacggaacat cttcgtgcgc gcgtcgtcgc tgaaacgtac
     1201 ttctttggct gccatgttac ttgccctcga tcacggccat gacgtcttcc tcgcgcatga
     1261 cgagcagatc ctcgccatcg accttgactt cagtgccgct gtacttgccg aagagaatct
     1321 tgtcgccgac cttgatgtcg agcggacgga ccttgccgtc ttcgagaatc ttgcccttgc
     1381 cgatggcgac gaccttgccc tggatgggct tttcggccgc gctatcggga atcacgatac
     1441 cgcccgggga ggtacgttcc tcctccaggc gcttcacgat cacgcggtca tgaagcggac
     1501 ggatcttcat ggaattcgct ccttggaagc tgatgctttg gttgattgct gtggagtcgt
     1561 tgcaggcgag tgttagcact ctccccgact ggctgctaat gataggggcc agttccgcga
     1621 attcaaggct tgcgccgcat ttctttacat aatgcgaccc atgggaaccc ggaatgcggc
     1681 gccggctcaa agcacggggc cgcgtggctc gcttaggatg gacggatgac caatcgcttc
     1741 cctcgcgcgc ggtggctcgc gctggccctc ctgctctcat ccccggcggc gagcgtgctc
     1801 gcggacgagt tcctgcctcc cgagcaggcc ttccgctaca gcctcgacag cgacgggcag
     1861 aagatcgtcg tccactggga cgtcgagccc ggctactacc tgtacctgaa acgcatgggc
     1921 gtcagctcga ccgccgaggg gctcaccctc ggtgaggcgg tttacccgca gggcgaggtg
     1981 cacaccgacg agtatttcgg cgagcaggtg gtcttccgcg gcagattcga cgtcacggtg
     2041 cccatcactg tcgcaccggg cacgtccgcg cgtgacatcc cggtcacgct gaaactgcag
     2101 ggctgtgccg acgcgggcct gtgctacccg cccacggcct gggacacctc cgtcacgatc
     2161 cccgccgccg gcgatgcaac ggttcccgcg gcaacgggcc tcgatgccct gatggcccag
     2221 gcgcgcagca cgggcggctt cggcgcaggg ggcgacgagt tcctcgatcc cgacgtcgcc
     2281 tttcgcctcg gcgccgtcgc cgacggcccg gatcgcgtgc gcctcacctg ggagatcgcc
     2341 gcgcagtact acctgtaccg cgaaaagctc ggcgtgaaga cgggcagcgc cgcgagcgtc
     2401 ggccagctgg tcctgccggc cggcgaggtg cacagcgacg agtatttcgg cgagcaggag
     2461 gtgtaccgcg aggagctcgt ggccacgata ccggtggcgc gtgcaggcgg cacggcgatg
     2521 acgatcgcgc tcgacgtgac ctaccagggc tgcgccgacg cgggcctgtg ctatccgccg
     2581 atcaccaaga cgctgaatgt cgatttgccc gccggcggaa caatgtcggc tgcttcgggc
     2641 ggtttcgtgt cgaagcagga caagatggcc gcgttcattc gctcaggcaa cctgctgttc
     2701 gtcatcgcct tcttcttcgg cgcagggctg ctgctggcct tcacgccctg cgtgctgccc
     2761 atggtgccaa tcctgtccgg gatcatcgcg ggccagggcg cgaacgtcac cacgcgccgc
     2821 gcgttcttcc tgtcgctgag ctacgtcctc ggcatggcct tcacctacac actcgccggc
     2881 atcatcgcgg ccgcggccgg cgcgcaggtg caggcggtat tccagcagcc gtggatcgtc
     2941 acggtctttg cgctgctgtt cgtggtgctc gcactgtcga tgttcggcct cttcacgatc
     3001 cagatgccgg cagcgatcca gacgcgcctc gccgacgcga gcaaccgcca gcgcgcgggc
     3061 accttcggcg gggtcgcggc gatgggtgcg ctgtcggccc tcatcgtcac gacctgcgtg
     3121 gcgccggcgc tcgtcgccac gctcgccgtc atcggccaga gcggcaatgt ggtgcgcggc
     3181 gggctcgcgc tcttcgccat gagcctcggc atgggcgcgc cgctgctcgc cgtcggcgcc
     3241 tccgccggca agctgctgcc gaaggccggc ccgtggatgg acacggtcaa gcaactcttc
     3301 ggcgtgctgt tcctgctggt cgcggcctgg atgctggcgc gcctcgtgcc gggaccgctc
     3361 gctctgctgc tgtgggccgt gccggcgctc gccggtgcat gggtgtgctt caaggcggcg
     3421 aagcaggcaa ggaagggccg gccctggtgg cgcagcgcag gcgccgtgac cggactctac
     3481 ggcgtcgtgc tgctcgccgg cgcggcgctc ggtggcggtg atccgctcgc gccgatcccg
     3541 cagctggcgg cgaaacacga ggaactcggg ttcaggcgca tcgcctcgct cgaggagctc
     3601 gagcgcgaag tggctgcggc ccacgccgcg gcacgcccgg tgatgctcga tttctatgcc
     3661 gactggtgcg tgtcgtgcaa ggaaatggag aaatacacgt tcacggaccc ggcggtgcgc
     3721 gacgcgctcg ccgccgccgt gctgctgcag gtcgacgtca cggcgaacaa cgagcaggat
     3781 caggcgctgc tgaagcactt cggcatctac ggaccaccga ccatcgcgtt ctacggcccg
     3841 gacggcatcg agcgacgcaa ctaccgcgtg gtcggctaca tgaaggcggc ggagttctcg
     3901 gcgctggcgc gcgaggcgac cgcgccctga gggccgatca gtcgagcggc gcgagcaggc
     3961 gcgcggcgtc ggctttcagg aacgacgcca ccgcagcacc cggcaccagc acttcgaagc
     4021 gcccctcggc gtacggcccg acgtcgtaag gcgagaacag cagctcgatg cccccgccct
     4081 cccccaggtc gtcggcgggc accagctgga aattctgcgc gaagcgcagc aggtccggca
     4141 cgaacggccc gtcaccctcg gccgcgatga aactgtccgc ttcgggggca tagtcgggga
     4201 tgcggctgcg cttgacctcg atgagcgcat cgcgcgccgc cgccgccagc gcggtgacgg
     4261 cctcggagcc aggccgcggg tcgcgcagca ggtccgcgag cgcaagccgg cgctggccga
     4321 ggcggtccca gacgaaggcg tcatagacgg tcagcgggtg cgcgccaccg gcgaactgcg
     4381 agatgagccc ccgcacgttg aggtagcgcc tgccggcagg gctgctggtc cactcggcct
     4441 tgaagaacag gccttccgcg ctcgcggcgc cgcgcgcctc ggcattgatc tgcgacatga
     4501 cctgcgtctc ggcgagcagg ccgcgcatgc gcggctcgat cagttcgtgc aggcgcggca
     4561 ccgcgacgat gccgccgtcg atcgtgatct cgccctcgaa gccgttcgcg ctcgcggtga
     4621 cgcgcacggg ctcagcgggc agggtcgccg gcagatcgag ctgcgtgcgt ccgcaggcag
     4681 cgagcatcag cgcgacgccc atggcggcgg caaggcggcg gattggcggg gacatggccc
     4741 agtctaaccg cgcacggcgg gcgcctgcac atggcacact tgggcgatga acggcatgca
     4801 gcgcttcctt gccctcgcgg ccatcatgtt gctggcgggc ctcgccggct atttcggcta
     4861 tcgcgcgacc ggcgactccg ccgcgccggt cacgggggtg gcgcagcccg cgacgacgtc
     4921 gcccgccgcg ggcgaggatg ccgcgctgcc gacgcgcccc gcgcgggaga tcccggagcg
     4981 cctgcccgag ttcacgctga aggatcgcga cggcgtgccg cgcacgctcg cgcactgggc
     5041 gggccgcccg ctgctggtga acttctgggc gacctggtgc gctccctgcc gccgcgagat
     5101 cccgttgctc acctcgctgc gcgctgagca tgccgacgag ggcctcgaag tcatcggcat
     5161 cgccgtcgac tttcgcgagg acgtgctgaa gtacgcggag gaaatcggcc tcgaataccc
     5221 gctgctgatc ggcgaacagg atggcctcga ggcggcggcg gcattcggcg tcgagccgct
     5281 gttcccgttc tcggtcttct ccgaccgcca gggacgcatc gtcgccgtgc ggatcgggga
     5341 gttgcatgcg gatgaagcag cgttcatcct cgaccgggtc cgggaagtgg gcgccaaccg
     5401 gctcgaactt cctgttgcgc agcagcaaat tgccgacaaa ctgcgcgaac tggccgtcct
     5461 tcgggcccag tctcccagct aaaagcgctg atcttgcagc gatcgtgcgc cgaagtatcg
     5521 cttacggcgc aaatccggta catttcgcgg cccatggcga gtctcctgct gctgaacggt
     5581 cccaacctga acctgcttgg ccagcgggaa cccgagacct acggctccgc caccctcgag
     5641 caactggtgg cgcgggcggc ggcgaccgca cgcagcctgg gccacgagct cgtcgccttc
     5701 cagagcaacg ccgagcatga actcgtcgat cgcgtccagg cggcacggag tgacggcacc
     5761 gcgttcatca ttctgaatcc gggcgccttc tcgcacacca gcatcgcgct gcgcgatgcg
     5821 ctgctcgcgg tcgccctgcc gttcatcgaa gtgcacctct cgaacgtgtt cgcgcgcgag
     5881 gccttccgcc atcactccta tttcaccgac atcgccgtcg gggccatcct cggcctcggc
     5941 ggcgccggtt acgaactcgc catccgcgcc gctgccgaac agctggccgc gcgcggcggc
     6001 caataatctc ctgcacggga ctcattcatg gacattcgca aggtcaagaa actgatcgag
     6061 ctgctcgagg aatcgggcat cgccgagatc gaaatcaggg aaggcgagga atccgtgcgc
     6121 atcagccgcg gctccacggc tccggcgcag caggtctacg ccgccgcgcc ctaccccgcg
     6181 cccgcgcccc aggccgctgc agcgcccgcg cccgcgcccg ccgcatcgcc gccgcccaag
     6241 tcgaatgaat acgtcgtgac ggcgccgatg gtcggcacgt tctacgcctc accctcgccc
     6301 ggcgcgaagc ccttcgtcga tatcggcgac gaggtcaagg tcggccaggt gctctgcatc
     6361 atcgaagcca tgaagatgat gaaccagatc gagagcgaca aggcggggcg catcacctcg
     6421 gtgatggtgc agaacggcga gccggtggaa ttcggccagc cgctctacac gatcgagtag
     6481 gccatgctgg acaaagtact gatcgccaat cgcggcgaga tcgcgctgcg gatcctgcgt
     6541 gcctgccgtg agctcgacat caagaccgtc gcggtgcact ccaccgccga cgcgaacctc
     6601 aagcacgtgc tgctcgccga cgagtcggtg tgcatcggcc cgccgccctc gccgcagagc
     6661 tacctcaaca tgccggcgat catcagcgcg gccgaggtca ccgacgccgt cgcgatccat
     6721 cccggctacg gcttcctctc cgagaacgcc gacttcgccg agcgcgtcga gaaaagcggc
     6781 ttcactttca tcggcccgaa gtccgaagtg atccggctga tgggcgacaa ggtctcggcc
     6841 atcaagatca tgaagagcca cggcgtgccc tgcgttccgg gctccgacgg cccgctcggt
     6901 cgcgacaacg acgagaacct gcgcatcgcc cgctcgatcg gctacccggt gatcatcaag
     6961 gcctcgggcg gcggcggcgg gcgcggcatg cgggtcgtgc
//
LOCUS       DAOQVY010000124         6960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_11960_length_6960_cov_5.041130, whole genome shotgun sequence.
ACCESSION   DAOQVY010000124 DAOQVY010000000
VERSION     DAOQVY010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6960
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11960_length_6960_cov_5.041130"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..863
                     /locus_tag="PKN91_10540"
     CDS             264..863
                     /locus_tag="PKN91_10540"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HNS28547.1"
                     /translation="MGGSSVRIRGFTLLELMIVVALAAVILAIGAPNFGEFRRNARLT
                     GDANDFLTAVQVARTEAVKQQRPVSLCATDDPQAADAACTVSADFGGWLVFTDPDTDC
                     SPAATTDITLRGGRAVADRGNVVSAADGACLSFAANGFARDEAPAGARRIVFCDERGY
                     ELQGGTGQSAARGIEVSRTGRGEVVREPARLEAWGLACE"
     gene            854..1366
                     /gene="pilV"
                     /locus_tag="PKN91_10545"
     CDS             854..1366
                     /gene="pilV"
                     /locus_tag="PKN91_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HNS28548.1"
                     /translation="MRMRQRGVSIVEALVALVVLSIGLLGIAGLFVESLRSSRTALLR
                     THAVNLASDIADRIRANASARDAYETATYGGAPAAHNCAPTASEAGRNCTVAELAEDD
                     LASWQEAVSLALPALPDPANAAVVEFEAGAAGRPDRYLITVAWQEPGARGAEEYRYTS
                     ELVIMERPPI"
     gene            1366..2487
                     /locus_tag="PKN91_10550"
     CDS             1366..2487
                     /locus_tag="PKN91_10550"
                     /inference="COORDINATES: protein motif:HMM:NF027400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HNS28549.1"
                     /translation="MKRQAARLRAAARGLSLIELLVALAIGSVLIVGAIYVYSRGRTT
                     YAVNESIARVQENGRYVLSVLEPDIELAGYYGFTNSPDTLRFVSGASPGTVYAIAAQM
                     RQQPPAPAVAVPVANLPAGAHECGTNFAVDVLTPVQGSNNDFALGPGAGGGCDPYGAG
                     AMPGADTLTIRRVETATTAPEAGRLQVYGSRLRSRSAQQLFVDGNAPGPVDDDNAVHN
                     LVVRAYYIARDAVDRPGLPSLRSKSLTTVGGATQFVEAEIMPGVEDFQVQFGIDTGDY
                     DNDGAIDPGADVNGDGIPETDGRATRYVNPDFPGLDLAQVVAVRLWIRVRADQPEPGF
                     RDDRTYRYADVEYTPDDDEARFRRVLMSRTISLRNSRTL"
     gene            2484..2993
                     /locus_tag="PKN91_10555"
     CDS             2484..2993
                     /locus_tag="PKN91_10555"
                     /inference="COORDINATES: protein motif:HMM:NF025699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HNS28550.1"
                     /translation="MNTRVPCRLPRAQRGAALVIGLVLLLVLTLLAVTGMNTATTELV
                     MAGNEQYRRAASLAASAGIEEAIADIGGVPTVPGAAPTVVSDAPTGNVAIDTYTTATR
                     YAGEESGLPQSSVDKFVGLHFEIDSTGRSARNARDRQVQGVFVVAGAAGSGGGSVGQI
                     GSGLGALGE"
     gene            2990..3358
                     /locus_tag="PKN91_10560"
     CDS             2990..3358
                     /locus_tag="PKN91_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28551.1"
                     /translation="MTRSLFMMTRNFSLIIAVALVALPVTAAEPARWVLEQAVEAEAG
                     SVLLPSGETGMIVVTSCEGCTPQSFVTTGKTRYLADGAPLALRDLRAGLASARHATVT
                     VLYDSRTREVTRVIANGLPR"
     gene            3355..6801
                     /locus_tag="PKN91_10565"
     CDS             3355..6801
                     /locus_tag="PKN91_10565"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HNS28552.1"
                     /translation="MNAPRRRAATLASATALSLLAGAGALADDTEIFVNQAALRDVKP
                     NILFIIDTSGSMDGEVQAQRPPYDPAASYGGACSADNVYWRESSAGSSAPPDCDSLQY
                     VAAASNRCAVAVTSLAGLAGTWTGALRRFETDAARWSNLLAGRADDLIECRTDSGVHG
                     ADAASTLRYAQDGEADAPWSANSNRQIDWNGATTYTLYSANWLNWYHSPPNPTRITRL
                     QTVQAVATALVGSIDDVNLGLMRFSANAEGGMVIHEIADIASARQSITERINELLPVG
                     STPLAETMYEAGQYFAGRLVDYGLASTSNAAGRLQPSVPESRDPQNAARYRSPIEYQC
                     QRNYTILLTDGEPTSDTGANTLIPALPDYAQLVGGGCTGTGHGACLPDMAEYLHDADL
                     SADLPGQQNVTTYTIGFGPEMVGSRLLAEVAARGGGEAYSASDVTDLTTTLQSIVGNI
                     LQTSSTFTTPSVSINAFNRTETLNDLYISVFTPRDTARWPGNLKKYAFRDGRIVDALG
                     NEAVDPATGFFRQGAQSFWSATPDGATVEAGGAVSRLPAEGERRVYTYVESGTSRNLT
                     SIGNRFERDNAALTAEVLGIEGDSPTREQLIDWARGIDVLDVDTDGDTSETNRFMGDP
                     LHARPALVTYGGSTANPNAEDGVVFVPTNDGFLHAVDSRTGRELWSFIAPELLPRLPT
                     LYRDAGVAARSYGLDGDVRILKFDVNQDGIVDSSAGDRVWIFFGMRRGGSTYYALDVT
                     DRDTPRLRWKIGPGDLPGVGETWSAPAIARVRVGGASQNGENLVLIFGGGYDGAQENG
                     DYVADTMGHRVFMVDAASGALLWYAGGPGGTGTPDLQIANMTNSIPGRVNVIDIDGDE
                     YADRMYAADMGGRIFRFDIFNGESRANLVTGGVFASLGAGDSATPTVTDNRRFYYAPD
                     VALIQRRAADPYYNLAIGSGYRGHPLALETRDRFYSLRDKTPFAKLTQSAYDNLTPIV
                     ESDLIDITDNPGVTPVPTEQRGWRLELRLNGGWTGEKVLAEAITVNGVILFPSYQPTP
                     PDRANPCLPATGLNRVYALGVDSGRPVIDFNDDEVLTADDLFVELAQSGIAGEVSFAY
                     ETVVGPGGGGGGGGGGDDGDLDELGRRALCVVGVEVLRKCVQPGGVVRSYWQRPEADS
                     GG"
BASE COUNT         1098 a         2517 c         2290 g         1055 t
ORIGIN      
        1 cgagatccat gcctcgcgcg gcgccgtcac cttctggccc accgcgcggg cgggcaccac
       61 cagtaccttc gaggtctgca gccggcttcc cggcgtggcc tcgcgcgccg tcatcgtcag
      121 ccagacgggg cgaccgcggc tgcgggtcgg cctgccccgc gggacacgat cgcgctgccc
      181 accgtgacgc cgcccgtccg ccgccggcag ccgatcgtcg gattccgtga gcccttgccg
      241 cgcacggggc tatagttgtt tttatggggg gaagttccgt tcgcatccgg ggtttcacgc
      301 tgctcgagct gatgatcgtg gtggcgctcg ccgccgtcat cctcgcgatc ggcgcgccga
      361 acttcggcga gttccgtcgc aatgcgcggc ttaccggcga cgccaacgat ttcctcaccg
      421 ccgtgcaggt cgcgcgtacc gaagcggtca agcagcagcg gcccgtctcg ttgtgcgcca
      481 ccgacgaccc gcaggcggcc gatgcagcct gcaccgtgtc tgccgacttc ggcggctggc
      541 tggtgttcac cgatccggat accgactgct cacctgccgc gaccaccgac atcacgctca
      601 ggggcgggcg agccgtcgcc gatcgcggca atgtcgtctc ggccgccgat ggcgcctgcc
      661 tgtcgtttgc cgcgaatggc tttgcgcgtg acgaggcgcc ggccggcgcc cgccgcatcg
      721 tgttctgcga cgagcgcggc tacgagctgc agggcggcac gggccagtcg gcagcgcgcg
      781 gcatcgaggt atcgcgcacg ggccgcggcg aggtggtgcg cgagccggcg cggctcgagg
      841 cctgggggct cgcatgcgaa tgaggcagcg cggcgtgagc atcgtcgagg cgctcgtcgc
      901 gctcgtggtg ctgtcgatcg gcctgctcgg catcgcgggt ctgttcgtcg agagcctgcg
      961 cagcagccgc acggcgctgc tgcgcacgca tgccgtcaat ctcgcgagcg acatcgccga
     1021 tcgcatccgc gccaatgcca gtgcccgcga cgcatacgag acggcgacct acggcggcgc
     1081 gccggcggcg cacaactgcg caccgaccgc cagcgaggcg ggccgcaatt gcaccgtcgc
     1141 ggaactcgcc gaagacgatc tcgcgagctg gcaggaggcc gtgagtcttg cgctgcctgc
     1201 cctgcccgac ccggccaatg cagcggtggt cgaattcgag gccggcgcgg ctggccgtcc
     1261 cgaccgctac ctgatcaccg tcgcctggca ggagcccggc gcccggggtg ccgaagagta
     1321 tcgctatacg tcggagctcg tgatcatgga aaggccgccg atctgatgaa gcgccaggca
     1381 gcaagacttc gcgccgccgc gcgcggcctg agcctcatcg agctgctggt ggcgctcgcg
     1441 atcggttcgg tgctgatcgt cggcgcgatc tacgtctact cccgcggccg cacgacctac
     1501 gcggtcaacg agtcgatcgc gcgcgtgcag gagaacggcc gctacgtcct ctcggtgctc
     1561 gaacccgaca tcgagctcgc cggttactac ggcttcacca attcgcccga taccctgcgt
     1621 ttcgtcagcg gcgcatcgcc cggcaccgtg tacgcgatcg ccgcgcagat gcgccagcag
     1681 ccgccggcgc cggcggtggc cgtgcccgtc gcaaacctgc ccgccggtgc gcacgaatgc
     1741 ggcacgaact tcgcggtcga tgtgctgacg ccggtgcaag gctcgaacaa cgactttgcg
     1801 ctgggcccgg gtgccggcgg cggctgcgat ccctacggcg cgggcgccat gccgggcgcc
     1861 gacacgctca ccatacgccg cgtcgagacg gcgaccacgg cccccgaagc gggacgcctg
     1921 caggtctatg ggtcacgcct gcgcagccgc tcggcgcagc agctgttcgt cgacggcaat
     1981 gcccccggcc ccgtcgacga cgacaatgcc gtgcacaacc tcgtcgtgcg cgcttattac
     2041 atcgcgcgcg atgcggtcga tcgccctggc ctgccctcgc tgcgcagcaa gtcactcacc
     2101 accgtcggtg gtgcgacgca gttcgtcgaa gcggaaatca tgccgggcgt cgaggacttc
     2161 caggtccagt tcggcatcga caccggcgac tacgacaacg acggcgcgat cgacccgggc
     2221 gccgacgtca atggcgatgg catccccgaa accgacggcc gcgccacgcg ctacgtgaat
     2281 ccggacttcc cgggactcga ccttgcccag gtcgtcgccg tgcgcctgtg gatacgcgtg
     2341 cgggccgacc agcccgagcc cggctttcgc gacgatcgca cctatcgcta cgccgacgtc
     2401 gagtacacgc ccgatgacga cgaggcgcgg tttcgtcgcg tactgatgtc gcgcaccatt
     2461 tcattgcgga actcgcgcac actatgaaca ccagagtccc ttgccgtctt ccccgggcgc
     2521 agcgcggcgc cgcgctcgtc atcggcctcg tgctgctgct ggtcctcacg ctgctcgccg
     2581 tcaccggcat gaacacggcg acgaccgaac tcgtcatggc cggcaacgaa cagtaccgtc
     2641 gtgccgcatc gctcgccgcc agcgccggca tcgaggaagc gattgccgac atcggcggcg
     2701 tcccgaccgt gcccggggcc gcgcccaccg tggtgtccga tgccccgacc ggcaacgtgg
     2761 cgatcgacac ctacaccacc gccacccgct acgcaggtga ggaatccggt ttgccgcaat
     2821 cgagcgtcga caaattcgtc ggcctgcact tcgagatcga cagcaccggg cgctcggccc
     2881 gcaatgcgcg cgatcgccag gtgcagggcg tgttcgtggt cgcgggcgcg gccggcagcg
     2941 gaggcggcag cgtgggccag atcggcagcg gcctcggtgc cctcggcgaa tgacccggag
     3001 cctgttcatg atgacccgca atttctcctt gatcatcgca gtcgcgctcg tcgctctgcc
     3061 cgtcacggcc gcggagccgg cgcgctgggt cctcgaacag gcggtcgagg ccgaggcggg
     3121 cagcgtgctg ctgccgtccg gcgaaaccgg catgatcgtc gtcacgagct gcgagggctg
     3181 cacaccacag tccttcgtga ccaccgggaa gacccgctat ctcgccgatg gcgcaccgct
     3241 cgcgctgcgc gacctgcgtg cgggcctcgc ctcggcccgc catgccacgg tgaccgtgct
     3301 gtacgactca cgcacacgcg aggtcacgcg cgtgatcgcc aacggattgc cgcgatgaac
     3361 gcaccacgcc gtcgtgccgc cactctcgcg tcggccaccg cgctgtcgct gctcgccggg
     3421 gcgggcgcct tggcggacga cacggagatc ttcgtcaatc aggcggcctt gcgcgacgtc
     3481 aagccgaaca tcctgttcat catcgacacc tccggcagca tggacggcga ggtgcaggcc
     3541 cagcgaccac cctacgatcc cgcggcgagc tatggcggtg cgtgcagcgc agacaatgtg
     3601 tactggcgtg agagcagtgc cggcagcagc gcgccgcctg actgcgattc attgcagtac
     3661 gtggccgcgg ccagcaaccg ctgcgccgtg gcggtcacga gcctcgcggg actcgcgggc
     3721 acctggaccg gcgcgctgcg acgcttcgag accgacgccg cccgctggag caacctgctc
     3781 gcgggtcgcg ccgacgacct catcgaatgc cggaccgaca gcggtgtgca cggcgccgac
     3841 gcggcctcga cactgcgtta tgcgcaggat ggcgaggccg acgctccgtg gtccgccaac
     3901 agcaatcgcc agatcgactg gaacggtgcc accacctaca cactctacag cgcgaactgg
     3961 ctcaactggt accactcgcc tccgaatccc acgcggatca cccgcctgca gaccgtgcag
     4021 gcagtggcca cggcgctggt cggctcgatc gacgatgtga acctcggcct gatgcgcttc
     4081 agcgcgaatg ccgagggcgg catggtgatc cacgagatcg ctgatatcgc gagcgcgcgc
     4141 caatcgatta ccgaacgcat caacgagttg cttccggtcg gctcgacgcc gctcgccgag
     4201 accatgtacg aggcgggcca gtatttcgcg ggccggctgg tcgactacgg ccttgcctcc
     4261 acctcgaacg cagcgggccg cctgcagccg agcgtgcccg aatcgcgcga cccgcagaat
     4321 gccgcgcgct atcgctcgcc gatcgagtac cagtgccagc gcaactacac gattctgctg
     4381 accgacggcg aacccacttc cgacaccggc gccaatacgt tgatcccggc cctgccggac
     4441 tacgcgcaac tggtcggcgg tggctgcacc ggcacaggcc acggtgcctg cctgcccgac
     4501 atggcggaat acctgcacga cgccgacctc tcggcagacc tgcccggcca gcagaatgtc
     4561 actacctaca cgatcggttt cggcccggag atggtcggtt cacggctgct cgccgaagtc
     4621 gcggcacgtg gcggcggaga ggcctactcg gccagcgacg tcaccgatct tacgaccacg
     4681 ctgcaatcga tcgtcggcaa catcctgcag accagttcga ccttcaccac gccgtccgtg
     4741 tcgatcaatg cgttcaaccg caccgaaacg ctcaacgatc tctacatctc ggtgttcacg
     4801 ccgcgcgaca cggcgcgctg gccgggcaat ctcaagaagt acgcattcag ggatggccgc
     4861 atcgtcgatg cgctcggcaa cgaggcggtc gatccggcga ccggtttctt ccgccagggc
     4921 gcgcagagct tctggtcggc aacgcccgac ggggccaccg tcgaggcagg cggcgccgtg
     4981 agccgcctgc ctgccgaggg cgagcggcgc gtgtacacct atgtggaatc cggcaccagc
     5041 cgcaacctca cctcgatcgg caatcgcttc gaacgcgaca acgcggcact cacggccgaa
     5101 gtcctcggca tcgagggcga ctccccgacc cgcgagcagc tcatcgactg ggcgcgcggc
     5161 atcgacgtgc tggacgtcga taccgacggc gacaccagcg agaccaaccg cttcatgggc
     5221 gacccgctgc acgcccgccc cgccctcgtc acctacggcg gcagcaccgc caacccgaat
     5281 gcggaagatg gcgtggtgtt cgtgccgacc aacgacggct tcctgcacgc cgtcgactca
     5341 cgcaccggac gcgagctgtg gtcgttcatt gcgcccgaac tgctgccgcg cctgccgacc
     5401 ctgtatcgcg acgccggtgt cgccgcgcgc agctacggcc tcgacggtga cgttcgcatc
     5461 ctgaagttcg acgtgaacca ggatggcatc gtcgactcga gcgccggcga tcgcgtgtgg
     5521 atcttcttcg gcatgcgccg cggcggcagc acctactacg cgctcgatgt cacggatcgc
     5581 gatacgccgc gcctgcgctg gaagatcggc ccgggcgacc tgccgggcgt cggcgagacc
     5641 tggtcggcgc cggcgatcgc gcgggtgcgc gtcggcggcg cctcgcagaa tggcgagaac
     5701 ctggtgctga tctttggcgg tggctacgac ggcgcacagg agaacggcga ctacgtggcc
     5761 gacaccatgg gccaccgggt attcatggtc gacgcggcga gcggcgccct gctgtggtac
     5821 gccggcggcc cgggtggcac cggcacgccc gacctgcaga tcgccaacat gaccaattcg
     5881 attcccgggc gcgtaaatgt catcgacatc gacggcgacg aatacgccga tcgcatgtac
     5941 gcggccgaca tgggcgggcg catcttccgc ttcgacatct tcaatggcga aagccgcgcc
     6001 aacctcgtta cgggcggcgt attcgcgagc ctcggcgccg gtgactcggc cacccccacc
     6061 gtcaccgaca accggcgctt ttactatgcg cccgatgtgg cgctgatcca gcgccgcgcc
     6121 gccgacccct actacaacct cgcgatcggc tccggctatc gcggccaccc gctcgccctc
     6181 gaaacgcgcg accgcttcta ctcgctgcgc gacaagacgc ccttcgcgaa gctcacgcaa
     6241 tcggcctacg acaacctgac tccgatcgtc gaaagcgatc tcatcgacat caccgacaat
     6301 cctggcgtca cccccgtccc gaccgagcag cgcggctggc gcctcgagtt gcgcctgaac
     6361 ggtggctgga ccggcgagaa agtgctcgcc gaggcgatca ccgtgaatgg cgtcatcctg
     6421 ttcccctcct accagcccac gccgcccgat cgcgcgaacc cctgcctgcc ggcgaccggc
     6481 ctcaatcgtg tctatgcgct gggtgtcgat tcgggtcgcc cggtgatcga cttcaacgac
     6541 gatgaagtac tcaccgccga cgacctgttc gtcgaactcg cgcaatcggg catcgcgggt
     6601 gaagtgagct tcgcctacga gaccgtggtc ggccctggcg gcggtggcgg cggcggtggt
     6661 ggtggcgacg acggcgacct cgacgagctc ggtcggcgcg ccctgtgcgt cgtcggcgtc
     6721 gaagtgctgc gcaagtgcgt gcagccaggg ggcgtcgtgc gcagctactg gcagcgcccc
     6781 gaggccgaca gcggcggttg acctgcgcgc ggcgacccaa ggcgtccgca tgtccggcga
     6841 cgaacgtgga gaacgccgag gagcggcgat cagcgctcgg cgtccgccat ggattctgct
     6901 cgtgccgatc tgatgcgccg agcgccgccg cgacgagggg acgccgccgc aggcggcgcg
//
LOCUS       DAOQVY010000125         6932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_12031_length_6932_cov_5.894431, whole genome shotgun sequence.
ACCESSION   DAOQVY010000125 DAOQVY010000000
VERSION     DAOQVY010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12031_length_6932_cov_5.894431"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PKN91_10570"
     CDS             complement(<1..496)
                     /locus_tag="PKN91_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNS28553.1"
                     /translation="MTPATVHTTALTGLTRLHSGKVRDIYAIDADTMLIVTTDRLSAF
                     DVILPDPIPWKGHVLNEIANYWFARTTHIVPNHLTGRAVEEVVADPEERALLAGRAVI
                     VRRLRALPIEAVVRGYLIGSGWKDYQHGGAVCGIALPPGLQLASRLPEPIFTPATKAE
                     VGAHD"
     gene            583..1362
                     /gene="djlA"
                     /locus_tag="PKN91_10575"
     CDS             583..1362
                     /gene="djlA"
                     /locus_tag="PKN91_10575"
                     /inference="COORDINATES: protein motif:HMM:NF006948.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HNS28554.1"
                     /translation="MATRWTGKIFGGLLGLFTLGPFGAALGVVLGHQYDQGTAQAGGA
                     RNFGAQFFTATFEVMGHIAKADGRVSEREIAAARAVMAALRLDEPSVRAAIACFQRGK
                     QPGFDYAGTVQALRRACIGRPDLLRIFFEIQMRAALDGTGLAPPTRGEVRRLAQWLGI
                     SPHEFAHIEAALRSGRAGGARPAAAPGISLEDAYAVLEVAAGATDAEVTRAYRRQLSR
                     HHPDKLKANGLPDSMIEHAKERTQRIIEAWDRIRAARGIRS"
     gene            1388..2062
                     /gene="rpe"
                     /locus_tag="PKN91_10580"
     CDS             1388..2062
                     /gene="rpe"
                     /locus_tag="PKN91_10580"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNS28555.1"
                     /translation="MRQTPWIAPSILSADFARLGEEVDAVLAAGADIVHFDVMDNHYV
                     PNLTIGPLVCEALRKHGVTAPIDVHLMVEPVDRIVPDFARAGATYISFHPEASRHVDR
                     TIGLIRESGARPGLVFNPATPLDWLDYTLDRIDLVLLMSVNPGFGGQSFIPSALGKLA
                     EARRRIDASGREVRLEIDGGVKVDNIGEIAAAGADTFVAGSAIFGAGNYKATIADMRR
                     AIAART"
     gene            2095..3897
                     /locus_tag="PKN91_10585"
     CDS             2095..3897
                     /locus_tag="PKN91_10585"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNS28556.1"
                     /translation="MPSRYLSLALYSLLITLVAGAWLVHGLRDAGDFPTAWIAAASIA
                     ACLAIWQFGIPVPRVGLTSMERVPQVGLLLVFSPPVAAALCGAAAFLWPLLNRSYSHG
                     SPRAAVLRAVHNGAMDTLMLLAAGSVYLAVGGRHPIAVPGPDDIVPLAAMALTAQAVN
                     VALLATYFRLDGRDVGRIIKPVYSLVDLVFVPAGVLAAVLYNTAAPATFVLFALLMVV
                     FVLSFNGIGSALAAADADSSPLARLSQAWRALRGARRLDDLGDRILAETRGLFRFDEF
                     LLAFVDREQRTVDLRVHERGGERLAPQARPLDSGLLGRVAARGDALLVEDWASAPDGL
                     RALADASEAGSLIIVALRDEGQVIGLLGVRHGRPGVYSDADLHLMEHLAEQVAPAIAD
                     ARAFEDLERYRRLLEQRVAERTDELARANRDKERLIAALDERSRTLERESQEDPLTGI
                     ANRRAFSRRLASEIEMSRASGRPLTLAVADLDFFKLVNDQLGHAVGDEVLRQCAAVMR
                     HICRATDHVARIGGEEFAIIFPGTDHRQALHSCEQLRRAVEQHGWHSLHADLRVTLSI
                     GVAEWDREAGADELQHAADSRLYGAKHSGRNRVA"
     gene            complement(4045..4680)
                     /gene="crp"
                     /locus_tag="PKN91_10590"
                     /pseudo
     CDS             complement(4045..4680)
                     /gene="crp"
                     /locus_tag="PKN91_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016506431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
     gene            complement(4757..5395)
                     /gene="coq7"
                     /locus_tag="PKN91_10595"
     CDS             complement(4757..5395)
                     /gene="coq7"
                     /locus_tag="PKN91_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HNS28557.1"
                     /translation="MHAASPLDNLLSAADRALRAVFAAPHASRPLPAAAPAADQTVPA
                     ADRQLAARLMRVNHAGEIAAQALYHGQALMARDAATREWLLDAAREEADHLAWCEQRL
                     RELDSRPSVLKPLWYAGSFAIGAAAAAISDRVSLGFVAETERQVESHLTDHLERLPPH
                     DARSRAILETMRADEVAHGARARDAGGVDLPAPVRALMRHASRVMTRTAYWI"
     gene            5532..5960
                     /gene="rplM"
                     /locus_tag="PKN91_10600"
     CDS             5532..5960
                     /gene="rplM"
                     /locus_tag="PKN91_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNS28558.1"
                     /translation="MTTVFAKPAEVRGDWFVIDAEGKTLGRLATQLAHRLRGKHKANY
                     SPHVDMGDHLVVVNAEKVRVTGNKLADKIYYHHTGYIGGIKSIALDKLLKEHPERVIE
                     IAVKGMLPKNTLGRNMFRKLHVFAGGKHPHTAQQPKPLEI"
     gene            5977..6369
                     /gene="rpsI"
                     /locus_tag="PKN91_10605"
     CDS             5977..6369
                     /gene="rpsI"
                     /locus_tag="PKN91_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNS28559.1"
                     /translation="MPATQNYGTGRRKTATARVYLKPGTGRITVNARPVDEFFGRETG
                     RMIVRQPLEVAQLTDKFDVQAHVSGGGITGQAGAIRHGITRALIEYDESLRRPLRAAG
                     FVTRDAREVERKKVGRRKARRGPQYSKR"
     gene            6392..6465
                     /locus_tag="PKN91_10610"
     tRNA            6392..6465
                     /locus_tag="PKN91_10610"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6424..6426,aa:Gln,seq:ctg)
     gene            6567..>6932
                     /locus_tag="PKN91_10615"
     CDS             6567..>6932
                     /locus_tag="PKN91_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28560.1"
                     /translation="MSDSTRFRWPVIAAACLLAAWLALGAAAGDAQTDIVASALFEGR
                     ALLAIDGAQRMLRVGETSPEGVKLLAANAREALVEVRGALHPDRAVPIPAEALGVATL
                     PPGVPARPLAALLAAGAVAL"
BASE COUNT         1014 a         2388 c         2408 g         1122 t
ORIGIN      
        1 cgtcgtgcgc gccgacctcg gccttggtgg ccggcgtgaa gatcggctcc ggcaaccgcg
       61 aggcgagttg caggccgggc ggcagcgcga tgccgcaaac ggcgccgccg tgctggtaat
      121 ccttccaccc cgagccgatc aggtagccgc gcaccaccgc ctcgatcggc agtgcgcgca
      181 gccggcgcac gatcactgcg cgtccggcga gcagcgcgcg ctcctccgga tcggcgacga
      241 cttcctcgac ggcgcgaccg gtcaggtgat tcggcacgat gtgcgtggtg cgcgcgaacc
      301 agtagttcgc gatttcgttc agtacgtggc ccttccacgg aatcgggtcg ggcaggatca
      361 cgtcgaaggc cgacagccgg tcggtcgtca cgatcagcat cgtgtcggca tcgatcgcgt
      421 agatgtcgcg gaccttgccg ctgtgcagcc tggtgagtcc cgtgagggcg gtggtgtgga
      481 cggtcgctgg tgtcatcggt tcgagggccc gcgcgcgcag ggccgggcat cttgaccgcc
      541 gctgcgcgat gcggtcaagg atcgtgtaac gttacggctt ccatggcgac tcgctggacg
      601 ggcaagatct tcgggggcct gctcgggctc ttcacgctcg gcccgttcgg tgccgcgctc
      661 ggcgtcgtcc tgggccacca gtacgaccag ggcacggcgc aggccggcgg cgcgcgcaac
      721 ttcggcgcgc agttcttcac cgccacgttc gaggtgatgg ggcatatcgc caaggccgac
      781 ggccgcgtct ccgagcgcga aatcgccgcc gcgcgtgccg tgatggcggc gctgcggctc
      841 gatgagccga gcgtgcgtgc cgcgatcgcc tgtttccagc gcggcaagca gccgggtttc
      901 gactatgccg ggaccgtgca ggcgctgcgc cgcgcatgca tcggccggcc cgacctgctg
      961 cgcattttct tcgagatcca gatgcgcgcg gcgctcgatg gcacgggcct cgcgccgccg
     1021 acgcgcggcg aggtgcgacg gctcgcgcag tggctcggga tttcgcctca cgagttcgcg
     1081 catatcgagg cggcgctgcg ctcgggacgc gccggcggcg cccgtccggc tgccgccccg
     1141 ggcatttcgc tcgaggacgc ctatgccgtg ctcgaggtgg cggccggtgc gaccgatgcc
     1201 gaggtgacgc gcgcctaccg gcgccagctc tcgcgccacc atccggacaa gctcaaggcg
     1261 aacggcctgc cggactcgat gatcgagcac gccaaggagc gcacgcagcg gatcatcgag
     1321 gcctgggacc ggatacgcgc cgcgcgcggc atccgcagtt agcgccggct cgcgtacact
     1381 tcgccccatg cgccagaccc cgtggatcgc cccgtcgata ttgtccgccg acttcgcgcg
     1441 gctcggtgag gaagtcgacg cggtactcgc cgccggggcg gacatcgtgc atttcgacgt
     1501 gatggacaac cactacgtgc cgaacctgac gatcggcccg ctcgtgtgcg aggcgctgcg
     1561 caagcacggc gtgaccgcgc cgatcgacgt gcacctgatg gtcgagccgg tcgatcgcat
     1621 cgtgccggac ttcgccaggg ccggggccac ctacatcagc tttcacccgg aggcgagccg
     1681 gcatgtcgat cgcaccatcg ggctgatccg cgagagcggc gcacgtcccg ggctcgtgtt
     1741 caaccccgcg actccgctcg attggctcga ctacacgctc gacaggatcg acctcgtgct
     1801 gctgatgtcg gtgaacccgg gcttcggcgg ccagagcttc atcccttcgg cgctcggcaa
     1861 actcgccgag gcgcgacggc gcatcgacgc ctcgggacgc gaggtgcgcc tcgagatcga
     1921 cggtggcgtg aaggtcgaca acatcggcga gatcgcggcg gcgggggccg acaccttcgt
     1981 cgcgggttcg gcgatcttcg gcgcgggtaa ctacaaggcg accatcgcgg acatgcgccg
     2041 cgcgatcgcc gcccggacct gaccgactcg cccagcgagc acgcagggac cgcaatgccg
     2101 tcgcgttatc tttcgctggc cctctactca ttgctcatca cactggtggc cggcgcctgg
     2161 ctggtacatg gcctgcgtga tgcgggcgat ttccccactg catggatcgc ggcggccagc
     2221 atcgcggcgt gcctcgccat ctggcagttc ggcatcccgg tgccgcgagt cggactcacc
     2281 tcgatggagc gcgtgccgca ggtgggtctg ctgctcgtgt tcagtccgcc ggtcgcggcg
     2341 gcgttgtgtg gtgccgcggc attcctgtgg ccgctcctga atcgcagcta cagtcacggt
     2401 tcgccgcgcg ccgcggtgct gcgcgccgtg cacaacggcg ccatggacac gctgatgctg
     2461 ctggcggcgg gcagcgtcta cctcgccgtc ggcggccgcc atccgatcgc tgtgcccggg
     2521 cccgacgaca tcgtgccgct cgccgcgatg gcgctcacgg cacaggccgt caacgtcgcg
     2581 ctgctcgcca cctacttccg gctcgacggg cgcgacgtcg ggcggatcat caagccggtc
     2641 tattcgctgg tcgacctcgt gttcgtgccg gccggcgtgc tcgcggcggt tttgtacaac
     2701 acggcggcgc cggccacttt cgtgctgttc gcgctgctga tggtcgtgtt cgtgctgagc
     2761 ttcaacggca tcggcagtgc gctcgctgcc gccgacgccg acagcagccc gctcgcgcgg
     2821 ctgtcgcagg cgtggcgcgc gctgcgtggc gctcgccgcc tcgacgacct gggggatcgc
     2881 atcctcgccg agacgcgcgg gctgtttcgc ttcgacgagt tcctgctcgc gttcgtcgac
     2941 cgcgagcagc gcacagtcga tctgcgcgtg catgaacgcg gcggcgagcg cctcgccccg
     3001 caggcgcgtc cgctcgactc gggactgctc ggccgcgttg ccgcgcgcgg cgatgccttg
     3061 ctggtcgagg actgggccag cgcgcccgac gggctgcgcg cgctggccga cgccagcgag
     3121 gcgggctcgc tgatcatcgt cgcgctgcgg gacgagggtc aggtgatcgg cctgctgggc
     3181 gtgcgccatg gccgcccggg ggtgtattcg gatgccgacc tgcacctgat ggagcacctc
     3241 gccgagcagg tggcgccggc cattgccgat gcgcgcgcct tcgaggacct cgagcgctac
     3301 cggcgactgc tcgagcagcg cgtcgcggag cgcacggacg aactcgcccg cgccaatcgc
     3361 gacaaggagc ggctgatcgc ggcgctcgat gagcgcagcc gtacgctcga gcgcgaatcg
     3421 caggaagacc cgctcaccgg gatcgcgaac cgccgcgcct tcagccggcg cctcgcgagc
     3481 gagatcgaaa tgtcacgcgc gtccgggcgg ccgctcaccc tcgcggtcgc cgatctcgac
     3541 ttcttcaagc tcgtcaacga ccagttgggg catgcggtcg gtgacgaagt gctgcgccag
     3601 tgcgcggccg tgatgcgcca catctgccgc gcgaccgatc acgtcgcgcg catcggcggc
     3661 gaggagttcg cgatcatttt cccgggcacg gaccaccgtc aggcgctgca ctcctgcgag
     3721 cagctgaggc gcgccgtgga gcagcacggt tggcactccc tgcatgcgga cctgcgggtg
     3781 accctgagca tcggcgtcgc cgagtgggat cgggaggcgg gcgccgacga gctgcagcac
     3841 gccgcggatt cgcgtctcta cggcgccaag cattccgggc gcaaccgggt cgcctgacgg
     3901 gccccgctgc cggcgactcc ctgtcgctca ggcctagtcg tcgtcctcat catcgtcatc
     3961 gtcatcgtcg acttgcggcc ggccctgggc ggccgcggcg ccgcccttga cgggcacgat
     4021 gaccgggcgc gtcttcatct tcgggcgcgc attgaacacc acgatggtct tgccggtggc
     4081 gcgcagcagg ccctggtcct ccagcacctt cagcacgcgg cccgccattt cgcgcgagca
     4141 gccgacgagg cgcgacagct cctggcggct caccttgatc tgcatgccct cgggatgcgt
     4201 catcgcatcg ggctcgccgc acaggtccat gatcgcgtgc gccacccggc ccgtgacgtc
     4261 gacgaacgcg aggtcgccga gcttgcgatt ggtgcggtcg aggcgcgtgg cgagctgggt
     4321 cgcgagctcg aacacgaggc ccgggctctc ggcggcaatc tggcgaaagc gcgggtaggt
     4381 catttccgcg agttcgcagg agttgcgcgt gcgaacccag gcgctgcgcg tcggggtctc
     4441 gtggaacagg cccatctcgc cgaagaactg gcccttgttg aggtaggcca gcaccatttc
     4501 gttgccttcc tcgtcctcga tcatcacctc gaccgagccg ctgatgatgt agtagagcac
     4561 gtccggcagg tcgccagcgt ggatgatcac ggtcttcgac ggcaccgtgc ggattcggca
     4621 atagctcagg aagcgattga tcgcaggtat gtcgctcaac ggcttgacgt tctcttccat
     4681 tttctccccc gggaccgcga ggcccacgct cgcagcgttt tagtatagat gtcggccccg
     4741 ctgcaagcgc ggggcatcag atccagtagg cggtgcgggt catcacacgc gacgcatggc
     4801 gcatcagtgc acgcacgggc gcgggcagat cgacgccgcc cgcgtcgcgg gcgcgcgcgc
     4861 catgcgcgac ttcgtcggcg cgcatcgtct cgagtatcgc gcggctgcgc gcatcatgcg
     4921 gcggcaggcg ttccaggtga tcggtgagat gcgactcgac ctggcgctcc gtctcggcga
     4981 cgaagccgag gctcacgcgg tcgctgatcg cggcagcagc ggccccgatc gcgaacgagc
     5041 ccgcatacca gagcggtttc agcacgctcg gccgcgagtc gagttccctg aggcgctgct
     5101 cgcaccacgc gaggtggtcg gcttcctccc gcgccgcatc gagcagccat tcacgcgtgg
     5161 cggcatcgcg cgccatgagc gcctggccgt ggtagagggc ctgtgcggcg atctcgccgg
     5221 catgattgac gcgcatcagg cgcgccgcga gctgccggtc tgccgcggga accgtctggt
     5281 cggctgccgg cgccgcggcg ggcaggggtc gggatgcgtg cggtgcagca aagacggccc
     5341 tcagggctcg gtcggcagct gaaagcaggt tgtcgagggg cgaggcggca tgcatgggcg
     5401 gacccattat aaagaggctt cgccatcggc ccgcactcct tttgcaaagc gcggaccgct
     5461 tgagtacact ttcgggcctt tttggggcct tccggctcga gcccggctgc gggcagttgg
     5521 agatcattga aatgactaca gtttttgcga agcctgcgga agtccgcggc gactggttcg
     5581 tgatcgacgc cgagggcaag accctggggc gtctcgcgac gcagctcgcc catcgcctgc
     5641 ggggcaagca caaggccaat tacagccccc acgtcgacat gggcgaccat ctcgtcgtcg
     5701 tgaatgctga gaaggtccgc gtcaccggca acaagctggc cgacaagatc tattaccacc
     5761 acaccggcta catcggcggc atcaagtcga tcgcgctcga caagctcctg aaggagcacc
     5821 cggagcgtgt catcgagatc gccgtcaagg gcatgctgcc gaagaacacg ctcgggcgga
     5881 acatgttccg caagctgcat gtgttcgcgg gcggcaagca cccgcacacc gcgcagcagc
     5941 cgaagccgct cgagatctga aggaagagaa acgaagatgc ccgccacgca gaattacggc
     6001 accggccgtc gcaagaccgc caccgcccgc gtttacctga agcccggcac gggccgcatc
     6061 acggtcaacg cgcgtccggt cgatgagttc ttcggccgcg agaccggccg catgatcgtc
     6121 cgccagccgc tcgaagtcgc gcagctcacc gacaagttcg acgtgcaggc gcacgtgtcg
     6181 ggcggaggca tcaccggcca ggccggcgcg atccgccatg gcattacccg cgccctcatc
     6241 gaatatgatg agagcctgcg ccgaccgctg cgtgccgccg gcttcgtcac gcgcgatgcg
     6301 cgcgaggtcg aacgcaagaa ggtcggccgc cgcaaggccc gtcgcggtcc gcagtactcg
     6361 aagcgctaag cgcagcaagc gctaccgatc ttgggggatc gtctagcggt aggacaacgg
     6421 actctgactc cgtttaccta ggttcgaatc ctagtccccc agccaacacg aagggcccgt
     6481 cgcgagacgg gccctttgtc gttgcgcagc gattgtggct atagtccggc ggtatcgccc
     6541 gccatgatcg acggcaagga ggtcccatgt ccgactcaac caggttccga tggccggtca
     6601 tcgcggcggc atgcctgctc gcggcatggc ttgcgttggg cgcagccgct ggcgatgccc
     6661 agacggacat cgtcgccagc gcgttgttcg aggggcgcgc actgctcgcc atcgacggtg
     6721 cgcaacgtat gctgcgcgtg ggtgagacct cgcccgaggg cgtgaagctg ctggcggcga
     6781 atgcacgcga ggcgctggtc gaggtccggg gcgcccttca tcccgatcgg gcggtgccga
     6841 tcccggccga ggcgctcggc gtcgcgaccc ttccgcccgg cgtgcccgca cggccgctgg
     6901 cggccctgct cgcggccggc gccgtcgccc tg
//
LOCUS       DAOQVY010000126         6925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_12045_length_6925_cov_5.366521, whole genome shotgun sequence.
ACCESSION   DAOQVY010000126 DAOQVY010000000
VERSION     DAOQVY010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12045_length_6925_cov_5.366521"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PKN91_10620"
     CDS             <1..674
                     /locus_tag="PKN91_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28561.1"
                     /translation="RGAYVRALGTILAAGNEAGLRAFREFLARYPDDAELRFETVRPF
                     LGALASGTASPDIVAIAGELRGEPRLVLDGIRHAARNEWDTLAALDPQLAAVGWTQPW
                     AVDALHVRVDWKTRVASPEVRRTYGEEALSLVDRALVVQPTSALYGLRVRAAISAARP
                     EVLVESIASYARSLSAAASQRTGTRRTLEQMAKLLDEAAQQSPENAPRARAVQAAVRV
                     SIDAL"
     gene            complement(671..817)
                     /locus_tag="PKN91_10625"
     CDS             complement(671..817)
                     /locus_tag="PKN91_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28562.1"
                     /translation="MSLAADQLVGTWRLEGDRLVLTADEGDAARGLARRHVIEWGRAG
                     CADT"
     gene            complement(814..1455)
                     /locus_tag="PKN91_10630"
     CDS             complement(814..1455)
                     /locus_tag="PKN91_10630"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNS28563.1"
                     /translation="MATFVIVRHAQASFGSQDYDALSALGITQAETTGRHFASRGAAF
                     DRVLVGPRVRHAETARRLVGPRRDFAAVDALDECGAGHGLMRHVLEHREGAPMRAVLE
                     RYLQLLEAWGRGAHEVPGCETRAQFGQRIAGWFDQACADLRQGEHVLVVTSAGVIALL
                     VSDLLQAPAPAWGQLMRTMRNASISELLVSGGRRSLASFNSTAHLEPAQVSGI"
     gene            complement(1466..1996)
                     /locus_tag="PKN91_10635"
     CDS             complement(1466..1996)
                     /locus_tag="PKN91_10635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNS28564.1"
                     /translation="MSASADSRALRDAFGLFATGVTIVTGRRAGGEPVGVTVNSFTSV
                     SLEPPLLLWCLQNASTSLDAFAVGHEFAVHVLSLEHEDEAMHFARRAAGKFPARYAGG
                     SGPPRIDGALCRLECLVEAQHAGGDHTIIVGSVTRCEVAGGAPLVFQGGVFGRFTPLP
                     RGRHVETWETFTGDWF"
     gene            complement(1993..3372)
                     /locus_tag="PKN91_10640"
     CDS             complement(1993..3372)
                     /locus_tag="PKN91_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HNS28565.1"
                     /translation="MAFLCTVLLPAPAGFSELAWPTLGLLAWMALWWVTEAVPLSVTA
                     LLPLLVVPLIGLPQPAQILTQYATSTIFLILGGFLIGLAMERWQLHRRIAYAIIAAVG
                     SHPRRLVLGVMIATAFVSMWVSNTSTTLMMLPVASSIVALVAAGDHGRATANFATAML
                     LGVAYAATIGGLGTLIGSPSNALVQGFMAQNFGHDFSFAAWMSFGIPTVVLLLPATWW
                     IVTRVALPFELPPDALPRDAMRRALHELGPMSPPERRVALIALVTAVLWISRPWLMGL
                     PGLHGVTDTVIAIAAGLALFLVPSGAGSGLALLDAEAMRKVPWDVLILFGGGLALAAA
                     IQASTLSDTLGVAMRGIAHLPQFAVTIAVVLVIVLWTEFNSNTATAATFMPVLATMAA
                     GNPETLLVLLAPAAMAASAGFMLPVGTPPNAIVFATGRITMRQMLRTGFLVDIAAVVV
                     ITVIAGVLL"
     gene            complement(3411..4391)
                     /locus_tag="PKN91_10645"
     CDS             complement(3411..4391)
                     /locus_tag="PKN91_10645"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNS28566.1"
                     /translation="MPLVAFAIVATGARATVYELPATGAALFGSVEHIVTAYEDTLID
                     LGRRYSLGYEEIRRVNPDVDPWLPGEGTAVLLPGQRILPSGPREGIVVNLPEHRLYYF
                     PKAKKGQPALVYTYPVSIGKMDWKTPLGRTRITQKEKNPNWYPPASVRKEHAARGEPL
                     PRVVPPGPDNPLGAYKMRLAIGGGSYLIHGTNNPAAVGMAITHGCIRMFPEDVEELFG
                     MVAVGTPVYLVNEPVKVAIVDGEVYLEAHPPVDAEGQTAAIDIEQFAAMLDAALGDSR
                     TAVHWDHARDILQAGNGMPQLIGVEADVDTDDAAIAVAPVPAGERPASRE"
     gene            4369..5235
                     /gene="cysQ"
                     /locus_tag="PKN91_10650"
     CDS             4369..5235
                     /gene="cysQ"
                     /locus_tag="PKN91_10650"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNS28567.1"
                     /translation="MAKATSGMVRKRLELDMTMPFYKNAEHDRIGAEFAVIAVRAGAA
                     IMAVYQTDFVTRMKADDTPVTLADQRAEELIVPALQALLPGVPVISEEAWDATQPAPA
                     PRGPFVLVDPLDGTREFVARNGEFTVNIALISDESPIAGCVYAPAIGELYYAGQRAWY
                     GGQPPGPDFDVARVQPIEARPLPQRGAIALASRSHRDPATDAFLATVPHLRIESVGSS
                     LKFCRIAAGRADLYARFGPTMEWDVAAGHAILQAAGGSVLAPDGATFKYGKFANGLRN
                     GPFIARGRDVTT"
     gene            5242..>6925
                     /locus_tag="PKN91_10655"
     CDS             5242..>6925
                     /locus_tag="PKN91_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNS28568.1"
                     /translation="MRISEISVHRPILALVISLLLLVIGLVSLTRLWATIREFPDINP
                     PIVSIDTRYLGASAEIIETKITQPIEERVAGLEGIEKLKSTSIDGRSRINLEFDLGRN
                     VDAAANDVRDRVSRILSALPDQADPPEIVKADSSAQPILFINMASDKMTTLELSDYVE
                     RNVADRFAAVPGVARVQFMGNRRYAMRIWLDAEALAARRLTVNDIESALRRENVQLPA
                     GRLESRTREFVLNTATGLTTPAEFRDLVVGRSPEGYLVRLGEVAEVTLAAENERTISR
                     TLGVAGITIGLEPQSNANALAVAAAAHAEVEAINADLPEGTQIGTNVDRSEFIKSSMR
                     EVLIALGISVLLVLVVIYAFLGSWRATLIPAVTIPVSIIGACAAIYAMGFTINTLTLL
                     GIVLAIGLVVDDAIVMLENIYRRIERGEASLLAAIDGSREIGFAVIATTLVLAAVFMP
                     ISFLTGRVGRLFGEFGLTLAASILFSCLVALTLTPMMTAQLFQRGAGRSRISETIDRL
                     FHRMSEHYRGLLVRSLARPWLIAGGALAVFAVAITLFVSLPEESTPQDDRGIV"
BASE COUNT         1164 a         2429 c         2268 g         1064 t
ORIGIN      
        1 cgcgcggcgc ctacgtgcgc gcgcttggca cgatcctcgc cgccggcaat gaggcaggcc
       61 tgcgcgcttt ccgcgaattc ctcgcgcgct atccggacga cgccgaactg cgtttcgaaa
      121 ccgttcgacc ctttctgggc gcgcttgcga gcggcacggc cagcccggat atcgtcgcga
      181 tcgcgggcga gttgcgcggc gaaccccggc tggtactgga cggcatccgc cacgccgccc
      241 gcaacgaatg ggacacgctc gccgcgctcg acccgcagct cgcggccgtc ggctggaccc
      301 agccgtgggc cgtcgacgcg ctgcacgtgc gcgtcgactg gaaaacccgc gtcgcatcgc
      361 cggaggtgcg acgcacctat ggcgaggagg ctctgtcgct ggtcgatcgc gcgctggtcg
      421 tacagcccac cagcgccctc tacggcctgc gcgtccgcgc cgcgatcagc gccgcgcggc
      481 ccgaagtcct ggtcgaatcg atcgcgagtt acgcccgctc actgtccgca gctgcctcgc
      541 agcgaaccgg cacgcgtcgc acgctcgagc agatggcgaa gctgctcgat gaagccgcgc
      601 agcagtcccc ggaaaacgcc ccgcgcgcgc gagccgtaca ggcggccgtg cgcgtctcga
      661 tcgacgccct ctaggtatcc gcgcaacctg cacggcccca ttcgatgaca tggcgtcgcg
      721 cgagcccgcg cgccgcatcg ccttcatccg ctgtcagcac cagccgatcg ccttcgagcc
      781 gccaggtccc gaccagttgg tcggcggcga gactcatatg ccgctcacct gcgccggttc
      841 gagatgcgcg gtggaattga agctcgcgag gctgcgtcgc cctcccgaga ccagcagttc
      901 gctgatcgag gcattgcgca tcgtgcgcat caactgcccc cacgccggag ccggcgcctg
      961 cagcagatcg gataccagca gggcaatgac acccgcggaa gtcacgacca gcacgtgctc
     1021 gccctgccgc aggtccgcgc aggcctggtc gaaccacccg gcgatccgct ggccgaactg
     1081 cgcgcgcgtt tcgcagcccg gcacttcgtg tgcaccgcgt ccccaggcct cgagcagctg
     1141 cagatagcgc tcgagtactg cgcgcatcgg ggcgccttcg cggtgctcga ggacgtgccg
     1201 catgaggccg tggcccgcac cgcactcgtc gagggcgtcg actgccgcga agtcgcgccg
     1261 cggcccgacg agccgacgcg cggtctcggc atggcgtacg cgcgggccga ccaacacgcg
     1321 atcgaacgcc gcgccccggc tcgcgaagtg gcggccagtc gtctcggcct gcgtgatgcc
     1381 gagcgcggac agcgcgtcgt aatcttggct gccgaacgaa gcctgcgcgt ggcggacgat
     1441 gacgaatgta gccactgcgt aaccttcaga accagtcgcc ggtgaacgtt tcccaggtct
     1501 cgacatgccg accgcgcggc aagggcgtga agcgcccgaa tacaccaccc tggaacacca
     1561 gcggcgcgcc gcctgccact tcacagcggg tcacgctacc tacaatgatc gtgtggtcgc
     1621 cgccggcgtg ctgcgcctcg acgaggcatt cgaggcggca aagcgcgccg tcgatgcgcg
     1681 gcgggccgct cccgccggcg tagcgcgccg gaaacttgcc ggcggcgcgg cgggcgaaat
     1741 gcatcgcctc atcctcgtgt tcgaggctca acacgtgcac ggcgaactca tggccgacgg
     1801 cgaaggcgtc gagactcgta ctggcgttct gcaggcacca cagcagcagc ggcggctcga
     1861 gcgacaccga agtgaacgaa ttcacggtga ccccgacagg ctcgccgccg gccctgcgac
     1921 cggtaacgat cgtgacgccc gtcgcgaaaa gcccgaaggc atcgcgcaag gcgcgcgaat
     1981 cggctgatgc actcacagca gcacgccggc aatgacggtg atcacaacca ccgccgcgat
     2041 gtcgaccagg aaaccggtgc gcagcatctg gcgcatcgta atcctgccgg tggcgaacac
     2101 gatcgcgttg gggggcgtgc cgacgggcag catgaagccc gcggaggccg ccatggcggc
     2161 cggtgcgagc agcacaagga gcgtttccgg attgccggcg gccatcgtcg cgagcaccgg
     2221 catgaaggtc gccgcggtgg cggtgttgct gttgaactcg gtccatagaa cgatcacgag
     2281 caccaccgct atcgtcacgg cgaactgtgg caagtgtgcg atgccgcgca tcgccactcc
     2341 cagagtatcc gacagcgtgc tcgcctgtat cgcggccgcg agcgcaagcc ccccgccgaa
     2401 aagaatcagc acgtcccatg gcaccttgcg catggcctcg gcatccagca gcgcgagacc
     2461 ggaacccgcg cccgaaggca cgaggaacag tgcgaggccg gcggcgatgg cgatcaccgt
     2521 gtcagtcacg ccatggaggc ccggcagacc catgagccat gggcgcgaaa tccatagcac
     2581 cgcggtcacg agcgcgatga gcgccacgcg ccgttccggc ggcgacatcg gcccgagttc
     2641 atgcagtgcg cgtcgcatgg catcgcgcgg cagcgcatcc ggcggcagtt cgaaaggcag
     2701 cgcaacgcgc gtcacgatcc accaggtcgc cggcagcagc aggaccacgg tcggaatgcc
     2761 gaacgacatc caggccgcga agctgaagtc gtggccgaag ttctgcgcca tgaacccctg
     2821 caccagtgcg ttggatggcg agccgatcag cgtgccgagc ccgccgatcg tggcggcata
     2881 ggcgacaccg agcagcatcg cagtcgcgaa attggcggtc gcgcgaccgt gatcgcccgc
     2941 cgcgacgagg gccacgatcg agctcgccac cggcagcatc atcagcgtcg tcgacgtgtt
     3001 cgacacccac atgctgacga acgccgtggc aatcatcacg ccgagcacga gccggcgcgg
     3061 atgactgccc acggcagcga tgatcgcgta ggcgatgcgc cgatgcagct gccagcgctc
     3121 catcgcaagt ccgatcagga atccgccgag gatcaggaag atggtcgagg tcgcatactg
     3181 ggtgaggatc tgcgccggct gcggtaagcc gatcagcggc acgaccagca atggcagcag
     3241 cgcggtgacg gacaggggca ccgcctcggt gacccaccac agcgccatcc atgcgagcag
     3301 gccaagcgtc ggccaggcaa gttcggagaa tcccgccggc gcgggcagca gcaccgtgca
     3361 cagaaaggcc acgggaccaa gcagcgcgac tgcgcggcgc aagggcccgg ctactcgcgt
     3421 gatgccgggc gttcgcccgc cgggaccggc gccacggcaa tcgccgcgtc atccgtatca
     3481 acatccgcct cgacgccgat cagttgcggc atgccgttgc ctgcctgcag gatgtcgcgc
     3541 gcgtggtccc agtgaacggc cgtgcggctg tccccgagtg ccgcatcgag catcgcggcg
     3601 aactgttcta tgtcgatcgc ggcggtctgt ccttcggcgt ccaccggcgg atgcgcctcg
     3661 aggtagacct cgccgtcgac gatggcgacc ttgaccggct cgttgacgag atagaccggc
     3721 gtgcccacag ccaccatgcc gaagagttct tcgacatcct ccgggaacat gcggatgcag
     3781 ccgtgggtga tcgccatgcc gaccgccgcc ggattgttcg tgccgtggat caggtagctg
     3841 cccccgccga tcgcgaggcg catcttgtag gcaccgagcg gattgtccgg acccggcggc
     3901 acgaccctcg gcagcggttc gccacgcgcg gcgtgctcct tgcgcaccga agccggcgga
     3961 taccagttgg ggttcttctc cttctgggtg atgcgcgtcc tgccgagcgg ggtcttccag
     4021 tccatcttgc cgatgctgac cgggtacgta taaacgagcg ccggctggcc cttcttcgct
     4081 ttcgggaagt aatagagccg atgctccggc aggttgacga cgatgccctc gcggggtccg
     4141 ctcggcagga ttcgctggcc cggtagcagc accgcggtac cctcgcccgg cagccacgga
     4201 tccacgtccg ggtttacgcg gcggatttcc tcatagccca ggctgtaacg ccgcccgagg
     4261 tcgatcagcg tgtcctcgta cgcggtcacg atatgctcta cgctaccgaa gagggcagct
     4321 ccggtggcag gcagctcata gacggtcgcg cgcgcgcctg tcgcgacaat ggcgaaggca
     4381 acgagcggca tggttcggaa aaggctggag ctggacatga ccatgccatt ctacaagaac
     4441 gcggagcatg accgaatcgg cgcggaattt gccgtgatcg ccgtgcgcgc cggcgcagcc
     4501 atcatggccg tgtaccagac ggatttcgtc acccgcatga aggcggatga cacgcccgtg
     4561 accctcgccg accagcgggc cgaggaactg atcgtgcccg cgttgcaggc tctattgccg
     4621 ggggtgccgg tgatctccga ggaagcctgg gacgccacgc agcccgctcc cgccccgcgc
     4681 gggccgttcg tgctcgtcga cccgctcgat ggcacacggg agttcgttgc gcggaacggc
     4741 gaatttaccg tcaacatcgc gctcatcagc gacgaatccc cgatcgcggg ctgcgtctac
     4801 gcaccggcca tcggggagct gtattacgcc gggcagcggg cctggtacgg cgggcagccg
     4861 ccaggccccg actttgacgt cgcgcgggta cagcccatcg aggcgcgccc cctgccccag
     4921 cgcggcgcaa tcgcccttgc cagccgctcg caccgcgacc cggccaccga cgccttcctc
     4981 gccacggtgc cacacctgcg gatcgaatcg gtgggctcgt cgctgaagtt ctgccgtatc
     5041 gccgccggcc gcgccgatct gtatgctcgc ttcgggccca cgatggagtg ggatgtagcg
     5101 gccggtcacg ccatcctgca ggcggcgggc ggctcggtgc tcgcgccgga tggtgcgacc
     5161 ttcaagtacg gcaagttcgc aaacggtttg cgcaacgggc ccttcatcgc acgtggccgc
     5221 gacgtcacga cctgatcacc gatgcgcata tccgaaattt ccgtccaccg accgatcctg
     5281 gcgctcgtca tcagcctgct gctgctcgtc ataggcctgg tctcgctcac gcgcctgtgg
     5341 gctacgatcc gcgagttccc cgacatcaat ccacccatcg tgtcgatcga cacgcgctac
     5401 ctcggcgcat ccgccgagat catcgagacg aagatcaccc agccgatcga ggagcgggtc
     5461 gccggcctcg agggcatcga gaagctgaag tcgaccagca tcgatggacg ttcgcgcatc
     5521 aacctcgagt tcgacctcgg ccgtaacgtc gacgcggcgg ccaacgacgt gcgcgatcgg
     5581 gtgtcgcgca tcctcagtgc cctgcctgac caggcggatc cgcccgagat cgtcaaggct
     5641 gacagcagcg cccaaccgat cctcttcatc aacatggcca gcgacaagat gaccacgctc
     5701 gagctgagcg actatgtcga gcgcaatgtc gcggaccgct tcgctgccgt gccgggcgtc
     5761 gcccgcgtgc agttcatggg caatcgccgt tatgcgatgc gcatctggct cgacgcggaa
     5821 gcgctggccg cccgccgcct gaccgtcaac gacatcgagt cggcactgcg tcgtgagaac
     5881 gtgcagctgc cggccgggcg gctcgaatcg cgcacccgcg agttcgtgct gaacaccgcc
     5941 accggtctca ccaccccggc cgagttccgc gatctcgtcg tcggtcgcag ccccgagggt
     6001 tacctcgtgc gcctcggcga agtcgccgag gtcacgctcg ccgccgagaa cgaacgcacc
     6061 atctcccgca cgctgggcgt cgccggcatc acgatcggcc tcgagccaca gtccaacgcc
     6121 aatgcgctgg ccgtggcggc ggcggcacac gccgaggtcg aggcgatcaa tgccgacctg
     6181 cccgaaggca cgcagatcgg caccaatgtc gaccgctcgg agttcatcaa gtcgtcgatg
     6241 cgcgaagtgc tgatcgcact cggcatttcg gtgctgctcg tgctggtcgt catctacgct
     6301 ttcctcggca gctggcgcgc gaccctgatt ccggcggtca cgatcccggt gtcgatcatc
     6361 ggtgcctgcg ccgcgatcta cgccatgggc ttcacgatca acacgctgac tctgcttggc
     6421 atcgtgctcg cgatcgggct cgtcgtcgac gatgcgatcg tgatgctgga aaacatctac
     6481 cggcgcatcg agcgcggcga agccagcctg ctcgccgcca tcgacggcag ccgcgaaatc
     6541 ggcttcgcag tgatcgccac cacgctggtg ctcgcggcgg tgttcatgcc gatctcgttc
     6601 ctcaccgggc gcgtcggcag gctgttcggc gaattcggtc tgacgctcgc cgcttcgatc
     6661 ctgttctcct gcctcgtggc gctgaccctg actccgatga tgaccgcgca gctgttccag
     6721 aggggcgcag gtcgcagccg catcagcgag acgatcgacc ggctgtttca tcggatgagc
     6781 gagcactatc gcggcctgct cgtgcgctcg ctcgcccggc cgtggctcat tgcggggggc
     6841 gcgctggcgg tcttcgcggt ggcgattacg ctgttcgtga gcctgcccga ggaaagcacg
     6901 ccgcaggatg atcgcggcat cgtgc
//
LOCUS       DAOQVY010000127         6883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_12141_length_6883_cov_5.924136, whole genome shotgun sequence.
ACCESSION   DAOQVY010000127 DAOQVY010000000
VERSION     DAOQVY010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12141_length_6883_cov_5.924136"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1792)
                     /locus_tag="PKN91_10660"
     CDS             complement(<1..1792)
                     /locus_tag="PKN91_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HNS28569.1"
                     /translation="MFQTGMPNMRTLLYFCLAIGSLAGCASRPATTTPAGPAIADVPA
                     RVETTPSAQNDGNDLAFWLHPADPARSLVLGTGGTAGLELFSLAGERIGAYDGIDPEL
                     VEVSHGLVIVADRRSGGLIVLRIDPDTLAVTPATRETLATQGEVGGLCAYRSPDTGVQ
                     YVFATVEGNVQQWQLGTTGDTVHGRIVRTFPVGVGSAYCAVDDATRSLFVSEETVGIW
                     RIAAEPETDAEREAIDLIAPAGHIEDEVKGLAVYRVDGDTAYLLAGDIKPARINVYGL
                     ADGAWIGSFRITAAGDIDAVGETEGLAAIAFPLAGSAGGLVGAFDEENEGASGNIKLA
                     SWDAIAAGLGLASASGRDPRIAPAPTARTVVPRAETAPVATWGDAADDPAIWVHPTDP
                     AKSLVITTNKKLGLDIHDLAGKRLSSLPIGRVNNVDLRDGFMLGGRSVTLLAASNRTT
                     QGISLLRLDPATRRLVDVAGGVIPTGLSDPYGLCMYRSAGSGHTYVFINDNNETGTMR
                     QWQLIAVGDKVRAEPVRSFEVGSLAEGCVADDATGALYVAEEDVALWRYSAEPDGGTE
                     RRAVAKVGPGELTDDAEGLAIRARPDGTGYI"
     gene            1882..3234
                     /locus_tag="PKN91_10665"
     CDS             1882..3234
                     /locus_tag="PKN91_10665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS28570.1"
                     /translation="MKALLRLFATGADQPQLTDTRRIDELFRRHRLRVMLAITLGYGL
                     VYTCRLALSVAKKPLIDLGIFSPSELGLIGSALFYAYAVGKLVNGFLADHANMKVFFA
                     FAVLMSALLNVGMGFSTILWVSVLLWGLNGWFQSFGAPAGVVAMANWFGNRERGRYYG
                     IWSTAHSIGEGLTFVAVGGLVAAFGWRYGFWGPGLLCVLVAVGMYLLVQDRPRTLGLP
                     TVADWRNDHWQTDEAHSRADVGVFRTQLSILKIPAVWVLALASASIYVSRYAINSWGV
                     LYLQETHGFSLVEAGTFLMVNTFAGIAGCLAFGFISDKWFNARRPPANLIFALAEIAG
                     LVLLFYGPHTTLSLMCAFILYGLGLNGLVTSLGGLFAVDIAPKRVAGAAMGLIGIFSY
                     IGAALQENISGHLIERGMTVAADGLRTYDFGPATTFWLGSSVVSMVLAATLWRVRLRD
                     "
     gene            3256..4155
                     /locus_tag="PKN91_10670"
     CDS             3256..4155
                     /locus_tag="PKN91_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNS28571.1"
                     /translation="MTITPIRCDFATLGAYTKDTIVSKAGTRHVDGGGYSYAAHAARL
                     ACVDVAAVTRLAAEDRRSTHLLVEAGVQVVIHESRASTLMRLEYPTDDVDQRVLTVAA
                     LADPFQPADIADVEAGAWLVSASVRGEVPLEVIQAIKATGALIGADVQGYVRVVGDDG
                     RLHYEPWPEQAAVLALVDVLKTDAVEAEFLTGTADIHRAAAILASQGPREIVLTHRDG
                     MLVLAEGREYEATFHPEPLRGRSGRGDTCIGSYLSRRLAHSPAEATIWAAAVTSLKME
                     AEGPIRRPVADVHALIERKYGAD"
     gene            4157..5461
                     /locus_tag="PKN91_10675"
     CDS             4157..5461
                     /locus_tag="PKN91_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNS28572.1"
                     /translation="MTTPSHWMAAPLTLLVAALLAACAAPQPAEIPKTSVPDSQVFGF
                     VAFGDSGYQYDYLDPEDIAADLDGFIALERAEWLEDRRVPAEFVPAPPHTLADGRVVA
                     ASGLAPVARAMRSYCAVENCRFALMLGDNIYPDGATAGADGRSDADRFNALFTEPFGD
                     YGALAAGFRIYAALGNHDWNTSRAGAEAQLRFLESTPPFYMDGYFYRVVPPGLGGEVE
                     IFVIDTELLLAHVPVPETEVDWDGSEILLPEYDDLSPGVARQSDAERAMATWLEQALA
                     SSTARWKIVMGHHPLWSTAGSKFAQARALRELILPTLCRHADLYLAGHEHTLEAHVDD
                     CSKALPGAHAQPLLQVVSGAAAKQRPSNALFAAKQQLNNPELRSLYSRGMVWGFAHVT
                     LESERATVRLISTPNDGSGRMIQEFEVVLARRSAGPSTSPQP"
     gene            5458..>6883
                     /locus_tag="PKN91_10680"
     CDS             5458..>6883
                     /locus_tag="PKN91_10680"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004951467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNS28573.1"
                     /translation="MSTPAIDTRHGAALLALGHYINGRQVAGASGGRHGNVYNPATGE
                     LRARVAFASQDETLAAIAAARAAAPAWAATPPLQRARVMFRFKALIEAHREELARLIT
                     LEHGKVLSDATGEVTRGLEVVEFACGIPQLLKGEFSEDVGAGIDSWSIRQPLGVCAGI
                     TPFNFPAMVPLWIIPMALACGNTFVLKPSEKDPSCPLRLAELLSEAGLPAGVFNVVNG
                     DREAVDTLLTHPDVAAVSFVGSTPVAEYIHATAAKHGKRVQALGGAKNHMVVMPDADP
                     ERVANALLGAAYGSAGERCMAISVAVTVGNSAEPLIAALRAKLATLRIGSGLEPDVEM
                     GPLVTGGHREKVEGYIAAGVAEGATLVVDGRGHRVKGHERGFFIGPTLFDHVAPAMRI
                     YREEIFGPVLAIVRAGTLAEALELVNAHEFGNGTSIFTNDGRAARMFAHGVAAGMVGV
                     NVPIPVPMAFHSFGGWKRSLFGDMH"
BASE COUNT         1043 a         2414 c         2313 g         1113 t
ORIGIN      
        1 cgatgtagcc ggtgccgtcc gggcgcgccc tgatcgccag gccttcggca tcgtcggtca
       61 actccccggg cccgaccttg gcgacggcgc ggcgctcggt gccgccgtcg ggctcggcgg
      121 agtaacgcca gagagcgacg tcctcctcgg cgacatacag cgcaccggtt gcgtcgtcgg
      181 ccacgcagcc ctcggcgagc gaaccgacct cgaaactgcg caccggctcg gcgcgcacct
      241 tgtcgccgac tgcgatcagc tgccactggc gcatcgtgcc ggtctcattg ttgtcgttga
      301 tgaacacgta ggtgtggccg ctgcctgcgc tgcgatacat gcagagcccg tagggatcgg
      361 acagtccggt cgggatgacg ccgcctgcga catcgacgag gcgccgtgtc gccgggtcga
      421 ggcgcagcag cgagatcccc tgcgtcgtgc ggttcgaggc ggcgagcagc gtgacggacc
      481 tgccgccgag catgaagccg tcgcgcaggt cgacattgtt cacgcggccg atgggcagcg
      541 aggacaggcg cttgccagcg aggtcgtgga tgtcgaggcc gagcttcttg ttcgtcgtga
      601 tcacgaggct ctttgccggg tcggtcgggt ggacccagat ggccggatcg tccgcggcat
      661 caccccaggt cgcgaccggc gcggtctccg cgcgcggcac gaccgtacgc gccgtgggcg
      721 ccggggcgat gcgcggatcg cggcccgagg cgctcgcgag cccgaggccc gcggcgatcg
      781 catcccagga ggcgagcttg atgttgccgg atgcgccctc gttctcctcg tcgaacgcgc
      841 cgacgaggcc gcccgccgag cccgcaagcg ggaaggcgat ggcggccagt ccttcggttt
      901 caccgaccgc gtcgatgtcg ccggcggcgg tgatgcggaa gctgccgatc caggcgccgt
      961 cggcgaggcc atagacattg atgcgcgcgg gcttgatgtc gcccgcgagc agatacgccg
     1021 tgtcgccgtc gacgcgatag accgcgagcc ccttcacttc atcctcgatg tggccggccg
     1081 gtgcgatcag gtcgatggcc tcgcgctcgg cgtcggtctc gggctcggcg gcgatccgcc
     1141 agatgccgac cgtctcctcg gagacgaaca gggaacgcgt cgcgtcatcc acggcgcaat
     1201 aggccgagcc cacgcccacc ggaaaggtgc gcacgattcg cccgtgcacc gtgtcacccg
     1261 tcgtgccgag ctgccactgc tgcacgttgc cctcgacggt cgcgaacacg tactgcacgc
     1321 cggtatccgg gctgcgataa gcgcacagcc cgccgacctc gccctgcgtg gcgagggtct
     1381 cgcgggtggc gggcgtgacg gccagcgtgt cgggatcgat ccgaagcacg atcaggccgc
     1441 cgctgcgccg gtcggccacg atcacgaggc catggctcac ttccaccagc tcgggatcga
     1501 tgccgtcata ggcgccgatg cgctcgccgg caagcgagaa cagctcgagg cccgccgtac
     1561 caccggtacc gagcacgagg ctcctggccg ggtccgcggg gtgcagccag aacgccaggt
     1621 cattgccgtc gttctgcgcg ctcggggtcg tctcgacgcg cgccggcacg tccgcgatgg
     1681 ccggccccgc gggcgtcgtg gtggccgggc ggctggcaca gcccgcaagc gagccgatgg
     1741 cgaggcagaa atacagaagc gtgcgcatat tgggcatccc ggtctggaac attttttcta
     1801 ttttgatatt ataatagaaa tgttatttca tgcaaaagaa acggcggcgg cgcgagtatc
     1861 gccgcaaggg gtgacggctc cgtgaaagcg ctgctccgcc ttttcgccac gggcgccgac
     1921 cagccgcagc tgaccgacac ccggcgcatc gacgagctgt tccgccgcca tcgtctgcgg
     1981 gtgatgctgg cgatcacgct cggctacggc ctcgtctaca cctgccggct cgcgctgtcg
     2041 gtggccaaga agccgctgat cgacctcggc atcttctcgc cgagcgaact gggcctgatc
     2101 ggctccgccc tcttctatgc ctacgcggtc ggcaagctgg tcaacggctt cctcgccgac
     2161 cacgcaaaca tgaaagtgtt cttcgcgttc gcggtgctga tgtccgcgct gctcaacgtc
     2221 ggcatgggct tctcgacgat cctgtgggta tcggtgctgc tgtggggcct gaacggctgg
     2281 ttccagagtt tcggcgcgcc cgcaggcgtg gtcgcgatgg ccaactggtt cggcaaccgc
     2341 gagcgcggcc gctactacgg catctggagc accgcgcact cgatcggcga gggactcact
     2401 ttcgtggccg tcggcggcct cgtcgcggcg ttcggctggc gctacggctt ctggggcccg
     2461 ggcctgctgt gcgtgctggt cgcggtcggc atgtatctgc tggtccagga tcgaccgcgc
     2521 acgctcggcc tgccgaccgt tgccgactgg cgcaacgacc actggcagac cgacgaggcg
     2581 cactcgcgcg ccgatgtcgg cgtgttcagg acgcagcttt ccatcctgaa gattcccgcg
     2641 gtgtgggtgc tggcgctcgc cagcgcgagc atctatgtct cgcgctacgc gatcaacagc
     2701 tggggcgtgc tgtacctgca ggaaacgcat ggcttctcgc tcgtcgaagc cggtaccttc
     2761 ctgatggtaa acaccttcgc cggcatcgcc ggctgcctcg ccttcggctt catctcggac
     2821 aagtggttca acgcccgccg accgcccgcg aacctgatct tcgcactcgc ggaaatcgcc
     2881 ggtctcgtgc tgctgttcta cggcccgcac accaccctga gcctgatgtg cgcgttcatt
     2941 ctctatggcc tcgggctgaa cggactcgtc acctcgctcg gcggcctgtt cgccgtcgac
     3001 atcgcgccga agcgggtggc cggcgccgcg atgggcctga tcggcatctt cagctacatc
     3061 ggtgcggcgc tgcaggagaa catcagcgga cacctgatcg agcgcggcat gacggtggct
     3121 gccgacggtc tgcgcaccta tgatttcggc cctgccacca cgttctggct gggctcgtca
     3181 gtggtttcga tggtgctcgc cgccacgctg tggcgcgtgc ggcttcgcga ttgaccgcaa
     3241 cccctggaga gtcacttgac gatcacgccc atccgctgcg atttcgccac cctcggcgcc
     3301 tacaccaagg ataccatcgt ttcgaaggcc ggcacgcgcc acgtcgacgg cggcgggtac
     3361 agctacgccg cgcacgcggc gcggctcgcc tgcgtcgacg tcgccgcggt cacgcgcctc
     3421 gccgccgagg atcggcgcag cacccacctg ctcgtcgagg ccggcgtgca ggtcgtgatc
     3481 catgaatcgc gtgcctcgac gctgatgcgg ctcgagtatc cgaccgacga cgtcgatcag
     3541 cgtgtgctga cggtggcggc gctcgccgac ccgttccagc ccgccgacat cgccgacgtt
     3601 gaggccggcg cctggctcgt gagcgcctcg gtgcgcggcg aggtgccgct cgaggtgatc
     3661 caggcgatca aggcgaccgg cgcgctgatc ggcgccgacg tgcagggcta tgtgcgcgtg
     3721 gtgggcgacg atggtcggct gcactacgag ccgtggccgg aacaggccgc ggtgctcgcg
     3781 ctcgtcgacg tgctgaaaac cgacgccgtc gaggcggagt tcctcaccgg caccgccgac
     3841 atccatcggg ccgcggcgat cctcgcctcg cagggcccgc gcgaaatcgt gctgacgcac
     3901 cgcgacggca tgctggtact ggccgagggg cgggaatatg aggccacgtt tcatccggag
     3961 ccactgcgcg gacgcagcgg tcgcggcgac acctgcatcg gctcttatct gtcgcgccgc
     4021 ctcgcacact cgcccgccga ggcgacaata tgggccgcgg cggtcaccag cctcaagatg
     4081 gaagccgaag gtcccatccg ccggccggtg gccgatgtcc acgccctgat cgagcgcaag
     4141 tacggagccg actgagatga cgacgcccag ccactggatg gccgcgcccc tcacgctgct
     4201 ggtcgcagcg ctgctggcgg cctgcgccgc gccgcagccc gccgaaatac cgaaaacctc
     4261 ggtgccggat tcccaagttt tcggcttcgt ggcgttcggg gactcgggtt atcagtacga
     4321 ttacctcgat cccgaagata tcgcggccga cctcgacggg ttcatcgcgc tcgagcgcgc
     4381 cgaatggctc gaggaccggc gcgtgcccgc ggaattcgta cccgcgcccc cgcacacgct
     4441 cgcggacggc cgcgtggtcg cggcgagcgg gctcgccccc gtggcgcgcg cgatgcgcag
     4501 ctactgcgcg gtcgagaact gccgcttcgc attgatgctc ggcgacaaca tctatcccga
     4561 cggcgcgacc gccggcgccg acggtcgcag cgacgcggat cgattcaatg ccctgttcac
     4621 cgaacccttc ggcgactacg gcgcccttgc cgccggtttc cgcatctacg ccgcactcgg
     4681 caaccacgac tggaacacct cgcgcgccgg cgccgaggcg cagctgcgct tcctcgagtc
     4741 gacgccgccg ttctacatgg acggctactt ctatcgggtc gtgcccccgg gtctcggcgg
     4801 cgaagtcgag atcttcgtga tcgacaccga actgctgctc gcgcacgtac ccgtgccgga
     4861 gaccgaggtc gactgggacg gcagcgagat cctgctgccg gaatacgacg acctctcgcc
     4921 gggcgtcgcc cggcagagcg acgccgaacg cgccatggcc acgtggctcg agcaggcgct
     4981 cgcctcctcg acggcacgct ggaagatcgt catgggccat catccactct ggtccacggc
     5041 aggcagcaag ttcgcccagg cgcgcgcgct gcgcgaactg atcctgccga ccctgtgtcg
     5101 ccatgccgac ctgtacctcg ccggacacga acatacactc gaggcgcacg tcgacgactg
     5161 ctcaaaggcg ctgccgggcg cgcatgcgca gccgctgctg caggtcgtct ccggagccgc
     5221 cgccaaacag cgcccgtcga acgccctgtt cgcggcgaag cagcaactca acaaccccga
     5281 attgcgctcg ctctactcgc gcggcatggt atggggcttc gcacacgtga ccctggagag
     5341 tgagcgcgcc accgtgaggc tcatatcgac gccgaacgat ggcagcggcc gcatgatcca
     5401 ggaattcgag gtcgtcctgg cacggcgctc tgccggccca tcaacgagtc cacagccatg
     5461 agtacaccgg caatcgatac ccgccacggc gcagctctgc tcgcgctcgg ccactacatc
     5521 aacggcaggc aggtcgcagg cgcgagcggc ggccgccacg gcaatgtcta caacccggcg
     5581 acgggcgaac tgcgcgcgcg ggtcgcgttc gcgagccagg acgaaaccct tgctgccatc
     5641 gccgcggcgc gcgccgccgc gccggcctgg gccgcgacgc caccgctgca gcgcgcgcgc
     5701 gtgatgttcc gcttcaaggc gctgatcgaa gcgcaccgcg aagagctcgc gcgcctgatc
     5761 acgctcgagc acggcaaggt actgtcggac gcgacgggtg aggtcacgcg cggactcgag
     5821 gtcgtcgagt tcgcctgcgg catcccgcaa ctgctcaagg gcgaattcag cgaggacgtc
     5881 ggcgccggca tcgacagctg gtcgatccgc cagccgctcg gcgtgtgcgc cggcatcacg
     5941 ccgttcaatt tcccggcgat ggtgccgctg tggataattc cgatggctct cgcctgcggc
     6001 aataccttcg tgctgaagcc gtcggagaag gatccctcct gcccgctgcg cctcgccgag
     6061 ctgctctccg aagcgggcct gcccgcaggc gtgttcaatg tcgtcaatgg cgatcgcgaa
     6121 gcggtcgata cactgctcac gcatcccgac gtcgccgcgg tgagcttcgt cggctcgacg
     6181 ccggtcgccg agtacatcca cgccactgcc gcgaaacacg gcaagcgcgt gcaggcgctc
     6241 ggtggcgcca agaaccacat ggtcgtgatg cctgacgccg acccggagcg cgtcgccaat
     6301 gcgctgctcg gtgccgccta cggctccgca ggcgagcgct gcatggcgat ctcggtggcg
     6361 gtcaccgtcg gcaactcggc cgagccgctg atcgcagcgc tgcgcgcgaa gctcgccacg
     6421 ctgcgtatcg gcagcggcct cgagcccgat gtcgaaatgg gcccgctggt cacgggcggg
     6481 catcgcgaga aggtggaggg gtatatcgct gccggcgtgg ccgagggcgc gaccctcgtc
     6541 gtcgacggcc ggggtcaccg ggtcaagggg cacgagcgag gtttcttcat cggtcccacg
     6601 ctgttcgacc acgtcgcccc cgcgatgcgc atctaccgcg aggagatctt cggcccggtg
     6661 ctcgcgatcg tgcgcgccgg cacgctcgcc gaggcgctcg aactggtgaa tgcgcacgaa
     6721 ttcggcaacg gcacctcgat cttcaccaac gacggccgcg cggcacgcat gttcgcgcac
     6781 ggagtcgcgg ccggcatggt cggcgtcaat gtcccgatcc cggtgcccat ggcgttccac
     6841 agcttcggcg gctggaagcg ctcgctgttc ggcgacatgc acg
//
LOCUS       DAOQVY010000128         6777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_12372_length_6777_cov_5.963553, whole genome shotgun sequence.
ACCESSION   DAOQVY010000128 DAOQVY010000000
VERSION     DAOQVY010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12372_length_6777_cov_5.963553"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1702)
                     /locus_tag="PKN91_10685"
     CDS             complement(86..1702)
                     /locus_tag="PKN91_10685"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNS28574.1"
                     /translation="MNRHDVIVIGAGHNGLTTAAFLAKAGLKVLVLERNPWIGGAAVS
                     RELHPGWLYSNCSYVCSLLRPEIYRGLDLARHGLQVAPYGGGLTFTRDGNFIGGYADK
                     DVRRREYARHNERDADAYLRYSRDTMRQTRFIRSLLLRTPPDPTSLRWRDLRELAHIA
                     REFARLGETTMGDTIRFYSMSIADYLNEYFESDLIKAHHAGSGIIGTALGVHSPGTAY
                     VLLHHYMGDVDGQIGSWGFARGGMGAVSNAIAGALRGAGGQIRVDAPVARIIVRGGET
                     KGVVLHNGDEIYADTVISSLDPKRTFLKLFDAGDLDADVVRKAREFRIRGSSGKLNIA
                     LDGLPSFPALGHGNPLIAGDMHFLDTLERFERAYDDWKAGTWSKDPYLDLLIPSLTDP
                     TMAPPGKHFMSVFVQYVPREVEGRDWTDADRAAFRETVFDQIARYSPDFRSLVLHAEV
                     RTPQELENEVGLTEGNIFQGELTFDQLFFNRPFPGYAQYRGPVRGMYMCGSATHPGGG
                     VMAAPGANAAREILRDLRRPSTVPEGWSDD"
     gene            complement(1719..2870)
                     /locus_tag="PKN91_10690"
     CDS             complement(1719..2870)
                     /locus_tag="PKN91_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNS28575.1"
                     /translation="MSIDVAQSLPRASDLDRTRGAFAGARTLPGEVYVSAEVLRLEQR
                     GIFARQWLCVGREADLPAAGDYMLKEIGGDSIIVVRAADGAIRAFYNVCRHRGARLLD
                     EREGHGLVRVLCPYHAWSYELDGSLHVAPRMGEDFCKSEHSLSPVRIGFHEGFIFLNL
                     DPAAVPLAKHLADLPDLGRFRMGELVRGKRIEYDVAANWKLICENYSECYHCAGAHPQ
                     LARISEQISRADRGQEIGACFNGGPMRLRDGVETMSMSGRSTLPTIPGLSHDDTRYVH
                     YYIIYPNMLLSPHPDYVLVHTAWPVAPDRTHVVCEWLFTAEAVAADSFDPSDVVQFWD
                     VTNRQDWALCERAQAGVGSRGYRPGPYQSSEDCVHLFDRWYADQLAALL"
     gene            complement(2943..4274)
                     /locus_tag="PKN91_10695"
     CDS             complement(2943..4274)
                     /locus_tag="PKN91_10695"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNS28576.1"
                     /translation="MKSVLLACGAAVLGACSASLDPELQDIDRAYVADSGTLALYCGA
                     MIDGVGDAPRSDVTLLVRNGRIASVTPGRSVPRGVAALDLPTHTCLPGLIDMHTHITE
                     RPDDTADLTVYFRRTRDEQLAISREFAEATLLAGFTTVRNVGVYVGWTDRALRDAIDA
                     GEVAGPRMQVAGYYLTIPGGGGDLLLPEVPEEQIPVAVRMGVARGPEEFRARAQAAVD
                     GGADLVKVIASGAVLAFGGVPGEPEMTPAELGVATEVAHAAARKVAAHAHGARSIKEA
                     ILAGADTIEHASLIDDEGIALARARDVALSMDVYNGDYIDTEGRRQGWPEEFLQKNAE
                     TTEAQRRGFTRAHAAGVAIVYGTDAGVYPHGLNARQFPIMVERGMTPMEAIKSATSVA
                     ARYMGWSDRVGELTPGHFGDVIAVEGDPLADIRRLQDVSVVVKGGLLFKRP"
     gene            4406..6415
                     /locus_tag="PKN91_10700"
     CDS             4406..6415
                     /locus_tag="PKN91_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNS28577.1"
                     /translation="MSNSPEQVGALDVALANTARLLQTDPRLAERQADEILAVLPGHP
                     GAELMLAIAARCQGRTAQAIATLETLARAHPDWCDAHWQLGLACVQAQETDAAIAALR
                     RAVALKPDLPDAWRLLADQLTLAGDVVAADEAYARHIRHSTRDPRLLRPATALCEGRL
                     PQAERDLRTHLKAFPTDLAAIRMLAEVGARLGRYGDAEALLRRCLELAPSFLAARHNL
                     AVVLQKEAKFAAALAEVELLLEAEPANPGYRNLAASVAARLGDMQRSIELYSEVLREF
                     PRQPKIWMSYGHTLKSAGRQDEAIAAYRRAVELVPQFGEAYWSLANLKTFRFSGIDIE
                     LMRQQLAVPGLRAEDRFHLDFALGKALEDAGEYAASFEHYASGNRIRRRGITWSAEEN
                     TDQLRRSRALFTREFFAAREGWGAPARDPIFIVGLPRAGSTLLEQILASHSQVEGTAE
                     LPDITQIARELGGRKRRTEVSRYPEILADLDADECRALGERYLAQTRAQRRTDAPIFI
                     DKMPNNFLHLGLIHLILPNARIVDARRHPLACCFSVFKQHFARGQGFSYDLGEVGRYY
                     RDYVELLDHFDTVLPGRVHRVFYERVVDDTEGEVRRLLAYCGLPFEPACLRFHENTRT
                     VRTASSEQVRRPIYRDGVDQWRHYAAWLAPLEAALGHALTDYPYP"
     gene            6442..>6777
                     /locus_tag="PKN91_10705"
     CDS             6442..>6777
                     /locus_tag="PKN91_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HNS28578.1"
                     /translation="MTRSRRRKLDRESARARAAGMYGVSAVSAAVMAAVAPAQAQTSG
                     GLEEVIVTAQKREESLQNVPLSIQAIGTEKLEQLHVTRFDDYVKYLPSVAYQSIGPGA
                     TAVYFRGVAS"
BASE COUNT         1112 a         2394 c         2260 g         1011 t
ORIGIN      
        1 caccttgcgg ccggcgcgcg cgaggtacgc cgcgcacacg aggccgttgt gaccgccacc
       61 gacgatgacg atgcgatcgg cgccttcagt catcgctcca cccctcgggc acggtcgacg
      121 gccgccgcag gtcgcgcagg atctcgcgcg ccgcattcgc gccgggcgcc gccatcacgc
      181 cgccgccggg atgggtcgcc gagccgcaca tgtacatgcc gcgtaccggg ccgcggtact
      241 gcgcgtagcc cgggaacggc cggttgaaga acagctggtc gaaggtgagc tcgccctgga
      301 agatgttgcc ttcggtgagc ccgacctcgt tctcgagctc ctgcggcgtg cgcacttcgg
      361 cgtgcagcac gagactcctg aaatcggggc tgtagcgggc gatctggtcg aacaccgtct
      421 cgcgaaacgc cgcgcgatcg gcatcggtcc agtcgcgacc ttcgacctcg cgcggcacgt
      481 actgcacgaa caccgacatg aaatgcttgc ccggcggcgc catggtcggg tcggtgagcg
      541 acggaatcag caggtcgagg tacggatcct tcgaccaggt gcccgccttc cagtcgtcat
      601 aggcgcgctc gaagcgctcc agcgtgtcga ggaaatgcat gtcgcccgcg atcagcggat
      661 tgccatggcc gagcgccggg aagctcggca acccgtcgag cgcgatgttc agcttgccgg
      721 aggagccgcg gatcctgaac tcgcgcgcct tgcgcacgac gtcggcgtcg agatcgccgg
      781 cgtcgaacag cttcaggaaa gtccgcttcg gatcgaggct cgagatgacg gtatcggcgt
      841 agatctcgtc gccattgtgc agcacgacgc ccttcgtctc gccgccacgc acgatgatcc
      901 gcgccaccgg cgcgtcgacc ctgatctgcc cgcccgcgcc gcgcagcgca cccgcgatcg
      961 cattcgacac ggcccccatt ccgccgcggg caaagcccca cgagccgatc tgcccgtcga
     1021 cgtcgcccat atagtggtgc agcagcacat aggcggtgcc gggcgagtgc acgccgagtg
     1081 ccgtgccgat gatgccgctg cccgcgtgat gcgccttgat caggtccgac tcgaagtact
     1141 cgttgaggta atccgcgatg ctcatcgaat agaagcggat cgtgtcgccc atcgtcgtct
     1201 cgccgagcct cgcgaactcg cgggcgatgt gcgcgagctc gcgcagatcg cgccagcgca
     1261 gcgaggtcgg atccggcggc gtgcgcagca gcagcgagcg gatgaaacgc gtctggcgca
     1321 tcgtgtcgcg cgaatagcgc agatacgcgt cggcatcgcg ctcgttgtgg cgcgcgtact
     1381 cgcggcggcg cacgtccttg tcggcatagc cgccgatgaa attgccatcg cgcgtgaagg
     1441 tgagccctcc cccatagggc gcgacctgca ggccgtggcg cgcgagatcg agcccgcggt
     1501 agatctccgg acgcagcagg ctgcagacat acgagcaatt cgagtagagc cagccgggat
     1561 gcagctcgcg gctcaccgcc gcaccgccga tccacggatt gcgttcgagc accagtacct
     1621 tgaggcccgc ttttgcgagg aaggcggccg tggtgaggcc gttatggccg gcaccgatga
     1681 cgatgacgtc gtggcggttc atgcagcggc gcgcgaggtc acagcagcgc ggccagctgg
     1741 tcggcgtacc agcgatcgaa caggtgcacg cagtcctccg aggactggta cggccccggc
     1801 cggtaaccac gcgaaccgac gccggcctgc gcacgctcgc acagcgccca gtcctggcgg
     1861 ttcgtcacat cccagaactg cacgacatcc gacggatcga aactgtcggc ggcaacggcc
     1921 tcggcggtga acagccactc gcacacgaca tgcgtccggt ccggtgcgac gggccaggcg
     1981 gtatgcacca gcacgtagtc gggatgcggc gagagcagca tgttcggata gatgatgtag
     2041 tagtgcacat agcgggtgtc gtcgtgcgag aggccgggaa tagtcggcag cgtgctgcgc
     2101 ccgctcatcg acatcgtctc gacgccgtcg cgcaggcgca tcgggccacc gttgaagcag
     2161 gcaccgattt cctggccgcg atccgcgcgg ctgatctgct cgctgatgcg cgcgagctgc
     2221 gggtgcgcgc cagcgcagtg gtagcactca ctgtaattct cgcagatcag cttccagttg
     2281 gcggcgacgt cgtactcgat gcgcttgcca cgcacgagct cgcccatgcg gaagcgcccg
     2341 aggtcgggca ggtcggcgag atgcttcgcg agcggcaccg ccgccggatc gaggttcagg
     2401 aagatgaagc cctcgtggaa accgatgcgc accggcgaga gcgaatgctc cgacttgcag
     2461 aaatcctcgc ccatgcgggg cgccacgtgc aggctgccgt cgagctcgta gctccaggcg
     2521 tggtaggggc acagcacgcg cacgaggcca tggccctcgc gctcatcgag caggcgcgca
     2581 ccgcgatgac ggcacacgtt gtagaacgcg cggatcgcgc catcggcggc gcgcacgacg
     2641 atgatcgagt ctccgccgat ttccttcagc atgtaatcgc cggccgccgg caggtccgct
     2701 tcacggccga cgcacagcca ctggcgcgcg aatatgccac gctgctcgag gcgcagcacc
     2761 tcggcggata catagacctc gcccggcaag gtgcgggccc cggcgaatgc cccgcgcgtg
     2821 cgatcgagat ccgaagcgcg cggcagggac tgcgcgacgt cgatggacat ggatgacccc
     2881 cgggagaacg gggccattgt acccgccggc cgtggcgctt gggcaccggc tgcgtggtgc
     2941 gctcaaggcc gcttgaacag cagcccaccc ttgaccacca ccgacacgtc ctgcagccgc
     3001 cggatgtcag cgagcgggtc gccctcgacc gcaatcacgt cgccgaagtg acccggtgtc
     3061 agctcgccca cgcgatccga ccagcccatg tagcgcgccg cgaccgaagt ggcggacttg
     3121 atcgcttcca tcggcgtcat gccgcgctcg accatgatcg gaaactgccg cgcattcagg
     3181 ccatgcggat agacgccggc atcggtgcca tagacgatcg ccacgccggc cgcatgcgcg
     3241 cgcgtgaagc cccggcgctg cgcctcggtg gtctcggcgt tcttctgcag gaactcctcg
     3301 ggccagccct gccggcggcc ttccgtgtcg atgtaatcgc cgttgtagac gtccatcgaa
     3361 agcgcgacgt cgcgcgccct ggcgagcgcg ataccttcgt cgtcgatcag cgaggcgtgc
     3421 tcgatcgtgt cggcgccggc gaggatcgcc tccttgatcg agcgcgctcc atgggcatgc
     3481 gcggccacct tgcgtgccgc cgcgtgcgca acttcggttg cgacaccgag ctccgcgggc
     3541 gtcatctcgg gctcgccggg tacgccgccg aaggccagca cggcgccgga cgctatcact
     3601 ttcaccaggt cggccccgcc atcgacggcc gcctgcgccc gggcccgaaa ctcctccggg
     3661 ccgcgcgcca cgcccatgcg caccgcgact ggaatctgct cttcgggcac ctcgggcaac
     3721 agcaggtcgc caccgccgcc gggaatcgtc aggtagtagc ccgcgacctg catgcgtggc
     3781 ccggccacct ccccggcatc gatcgcatcg cgcagcgcgc gatcggtcca gccgacatag
     3841 acgccgacgt tgcgcaccgt cgtgaagccg gcgagcagcg tcgcttctgc gaactcgcgc
     3901 gagatcgcga gctgctcgtc ccgcgtgcgc cggaagtaca cggtgaggtc ggcggtgtcg
     3961 tcgggccgct cggtgatgtg ggtgtgcatg tcgatcaggc ccggcaggca ggtgtgcgtc
     4021 gggagatcga gcgccgcgac accgcgcgga acgctgcgcc ctggtgtcac gctggcgatg
     4081 cgcccgttcc ggaccaggag cgtcacgtcc gaccggggtg cgtcaccgac gccgtcgatc
     4141 atcgccccac agtacagcgc cagcgtgccg gaatcggcca cgtaggcccg gtcgatgtcc
     4201 tgcagttccg gatcgaggga ggccgagcag gcgccgagca cggccgcgcc acaggccagg
     4261 agcacggatt tcatgggccg gcattatgcg ccccctgctg cgtcgggcgg gtggcgggtg
     4321 tagcccggat gcaacaggtt tgacaaagcg ttcgcgtggg attctgattg ccgttgcgtg
     4381 tcctcaccct gcaccagaca acggaatgtc taactcaccg gagcaggtcg gcgcgctcga
     4441 cgtcgcgctg gccaacaccg cgcgccttct ccagacggat ccacggctcg cggaacggca
     4501 ggcagacgag atcctggctg tgctccccgg ccatcccggc gccgaattga tgctcgccat
     4561 cgccgcccgc tgccagggtc gcacggcgca ggcgatcgcg acgctggaaa cgctggctcg
     4621 ggcgcacccg gactggtgcg acgcccactg gcaactcggg ctcgcctgcg tgcaggcaca
     4681 ggagaccgat gccgccatcg cggccttgcg ccgcgccgtg gccctgaagc ccgacctgcc
     4741 agatgcctgg cggctgctcg ccgaccagct caccctggcc ggcgatgtgg tcgccgccga
     4801 cgaggcctat gcccggcaca tccgccactc cacgcgcgac ccgcggctgc tgcggccggc
     4861 caccgcattg tgcgagggac gcctgccgca ggcggagcgc gacctgcgca cgcacctcaa
     4921 ggcattcccg acggatctcg cggcgatccg catgctcgcc gaagtcggcg cccggctcgg
     4981 ccgctacggc gatgccgagg cactgctgcg ccgctgcctc gaacttgcgc cgagcttcct
     5041 cgccgcgcgg cacaacctcg ccgtcgtgct gcagaaggag gcgaagttcg ccgcggcgct
     5101 tgccgaggtc gagctgctgc tcgaggcgga gccggccaac cccggctacc gcaatctcgc
     5161 ggcatcggtc gcggcgcggc tcggcgacat gcagcgctcg atcgagctct acagcgaggt
     5221 gctgcgcgaa tttccccgcc agcccaagat ctggatgagt tacgggcata cgctgaaatc
     5281 ggccggccgc caggacgagg cgatcgccgc ttatcgtcgc gcggtcgaac tcgtcccgca
     5341 gttcggcgag gcgtactgga gcctcgcgaa cctgaagacg ttccgcttca gcggcatcga
     5401 catcgaactc atgcgccagc agctcgccgt ccccgggctg cgggccgaag accgcttcca
     5461 cctcgatttc gcgctcggca aggccctcga agacgccggc gagtacgccg cgtcattcga
     5521 acactacgcc tccggcaacc gcatccgccg ccgcggcatc acctggtccg ccgaggagaa
     5581 caccgaccag ttgcgccgca gccgcgcgct gttcacccgc gaattcttcg cggcccgcga
     5641 gggctggggt gcgccggcgc gtgacccgat cttcatcgtt ggcctgccgc gcgccggctc
     5701 cacgctgctc gaacagatcc tcgccagcca ttcgcaggtc gagggcaccg cggaactgcc
     5761 ggacatcacg cagatcgcgc gcgaactcgg cgggcgcaag cgccgcacgg aagtctcgcg
     5821 ctacccggaa atcctggccg acctggatgc cgacgagtgc cgcgcgctcg gcgagcgcta
     5881 cctcgcccag acgcgagccc agcgccggac cgatgcgccg atcttcatcg acaagatgcc
     5941 gaacaacttc ctgcatctcg ggctcatcca cctcatcctg ccgaatgcgc gcatcgtcga
     6001 cgcgcgccgg catccgctcg cttgctgctt ttcggtgttc aagcagcatt tcgctcgcgg
     6061 ccagggcttc agctacgacc tcggcgaggt cggccgctac taccgcgact acgtcgaact
     6121 gctcgatcac ttcgacacgg tgttgcccgg ccgggtgcat cgcgtgttct acgaacgggt
     6181 ggtcgacgac accgagggcg aggtgcgacg cctgctcgcc tattgcggcc tgccgttcga
     6241 gccggcctgc ctgcgcttcc acgagaacac gcgcaccgtg cgtaccgcga gctccgagca
     6301 ggtacggcgc cccatctacc gcgacggcgt cgaccagtgg cgccattacg ccgcgtggct
     6361 tgcgccgctc gaggcggcac tgggccatgc actgaccgat tatccgtacc cgtagaaccc
     6421 aaggaaaagc cagggggaat catgaccaga agcagacgca ggaagctcga cagggagagc
     6481 gcccgcgcgc gcgcggcggg catgtacggc gtcagcgccg tctccgcggc cgtgatggca
     6541 gcagtggcgc ccgcgcaggc acagacatcg ggcggcctcg aagaggtgat cgtcaccgcg
     6601 cagaaacgcg aggagagcct gcagaacgtg ccactcagca tccaggcgat cggcaccgag
     6661 aaactcgagc aactacacgt gacgcgcttc gacgactacg tgaagtacct gccgagcgtc
     6721 gcataccagt ccatcggccc gggcgcgacc gcggtgtact tccgcggcgt ggcgagc
//
LOCUS       DAOQVY010000129         6631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_12738_length_6631_cov_5.371350, whole genome shotgun sequence.
ACCESSION   DAOQVY010000129 DAOQVY010000000
VERSION     DAOQVY010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12738_length_6631_cov_5.371350"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PKN91_10710"
     CDS             complement(<1..202)
                     /locus_tag="PKN91_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28579.1"
                     /translation="MRICVDLDGVIAALRRPGETYAGDAPVQWMNMSIGRSDMPALGR
                     AEDPTGSVDLGDDRVGASLSRVP"
     gene            complement(199..579)
                     /locus_tag="PKN91_10715"
     CDS             complement(199..579)
                     /locus_tag="PKN91_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNS28580.1"
                     /translation="MTELLNALLDISRLESGAITPLQTDVRLKDVFAELRDEFEPMAE
                     EKGIRLRIDGPAPVVSTDRILFSQIFQNLLGNAFKYTERGSVSLSAAFEEDAFVITVA
                     DTGVGIPADKLGQIFDEYFHGGVA"
     gene            complement(604..1152)
                     /locus_tag="PKN91_10720"
     CDS             complement(604..1152)
                     /locus_tag="PKN91_10720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS28581.1"
                     /translation="MGESNAITEYLLQFSPDAVIVVDDQGRIRFANETVREVLGHDPG
                     ALLGRPLDHLIPERLRERHGQLTAGFLRSPRKREMGAQVPDLLALCADGTELAADIRL
                     SPFRVDGRLYVAAAIRDNSERRQFSEKLSAARDVAERANRAKSRFLAMASHDLRQPMQ
                     TLRLLNAAMLKMSSPPDVQEIL"
     gene            1489..2196
                     /locus_tag="PKN91_10725"
     CDS             1489..2196
                     /locus_tag="PKN91_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNS28582.1"
                     /translation="MQIARISALEEMASGFAHELNQPIGAITTYAQAGVRMLSQPGPM
                     VASAIEVLRLISDEALSAGQGIHRIRGQFDSRTFPRETCDLADVLMELRPVLDLLAIR
                     AGLELAFTLEPGLPSVSVDRLRIQHVLFALVQNALEVPRRDSAVPEVRIEVTGDRYGV
                     LTAVIDGGAGVSAEAHENLFRPFYTTKRDGTGLGLASSRAIIEAHDGTIGCENVTGGG
                     TRFWFRLPAASREDIAQ"
     gene            2193..2831
                     /locus_tag="PKN91_10730"
     CDS             2193..2831
                     /locus_tag="PKN91_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNS28583.1"
                     /translation="MTAAPHQDPKSKPTIYIVDDDDGMRRALTLLMATVGYNPVAFAR
                     PSEFLAKYDPSLAGCLVIDVRMPEMSGLEVQQQLNRSGSMLPVILITGHGDIPMAVQA
                     MKNGASDFLQKPFRDQDLLDSINAALKQDAENRTSVERLTDLRRRVESLTPREQEVMD
                     LVVDGRANKVIAIDLGLSERTVEIHRANVMAKMGARSVAHLVKMCLMLGEPA"
     gene            complement(2834..4771)
                     /locus_tag="PKN91_10735"
     CDS             complement(2834..4771)
                     /locus_tag="PKN91_10735"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HNS28584.1"
                     /translation="MSPRRLSPDSQALIGLLCGAVLLGVVLALEYYCVQQVRVTFEQL
                     QRTQERRLTLETISEAILGMETGQRGYLLTSRGEYIEPYIAGQVQLAARLPVLQRLLA
                     EDGDSGQLENFRALERLLDTRRKQMDQVLDLQRAQGLDAALAFVTTNQGHFVMDAIRQ
                     LLDRMSAVELRQLDARMASSQTSLGRNLAFSTLLSIMAIALMVVILLLVRREFARRVE
                     AARQLDAANASLELRVRDATEGLSAANAVLEAEVRQRRAAEAELQREHELLDARVTER
                     TAELVEAGESKSRFFSAASHDLRQPLHSLTLLNATLRSQPLAQPVRDIVEAQQQMIDS
                     MGRMIHALLNISMLESGVVRPQIRDFPLQEIMAPLRAEFAPLADARGLSLEFATDSPV
                     VKSDPTLLKEVLQNLVGNAVRYTERGGVRVGVERTADRVLIEVRDSGPGIPVADLDLV
                     FEPFHQLRSQGRPREGFGLGLAVVRQLVKVLGIELKVESTVGVGTSFTIEVTPGRESK
                     PAGVRAAVAGGVPRRGGGILLVDDEATVLQATAIFLRVEGHEVITAGSPDEALAKLGR
                     LDRAPDLIVSDFQLGLPVTGAELIERVRATVGRDIPAVVLSGDMLRVVRHCEHLANCR
                     VFHKPVDAEELAQHLRATLEA"
     gene            complement(4785..5132)
                     /locus_tag="PKN91_10740"
     CDS             complement(4785..5132)
                     /locus_tag="PKN91_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNS28585.1"
                     /translation="MYKKILVPVDGSATSLRGLNEAVGLAKSCGAMLDIVHVVNEQVF
                     DTGYMPAPFYEQLFESQREGGRKVLAAADLIVMGTHGRRGLRRLAMGSDAELVVRSSP
                     VPVLLVRDQPEVP"
     gene            complement(5237..6235)
                     /locus_tag="PKN91_10745"
     CDS             complement(5237..6235)
                     /locus_tag="PKN91_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNS28586.1"
                     /translation="MSPSPAAGHRWALVLAAGDGARLQALTTIAAGISVPKQFCSLGG
                     GGSLLNAALQRARVIAPPARTCVIVAAHHERWWRTLPRSIEARNIVVQPRNRGTANGI
                     LLPLLQILHRDPEASLLVLPSDHYVRDEAVLAASLEQLMKETERQRGLIVLLGFAPEQ
                     ADAELGYIVPSDDRGPGARGVCQFVEKPSGKTAEALIERGGLWNSFIFAAHGRALLGA
                     FEERCPEVVGEMRAIVVATNDEALRQARLAELYERLPVLDFSRDIVERCTPRLRVLAV
                     PSCGWSDLGTPGRVAAAVECAPASLPTAARAVAQVHARGVLDLAAQHLRLQQAAAG"
     gene            complement(6487..6562)
                     /locus_tag="PKN91_10750"
     tRNA            complement(6487..6562)
                     /locus_tag="PKN91_10750"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6526..6528),aa:Glu,seq:ttc)
BASE COUNT         1103 a         2280 c         2183 g         1065 t
ORIGIN      
        1 ccggaactcg gctcaaggac gccccgacgc ggtcgtcgcc caagtccacg ctgcctgtgg
       61 gatcttctgc gcggcccagg gcgggcatgt cggagcggcc gatgctcatg ttcatccatt
      121 gcaccggcgc gtcgccggcg taggtctcac ccggcctgcg gagcgcggcg atgacgccgt
      181 ccagatcgac gcagatcctc acgcgacgcc tccgtggaag tactcgtcga agatctgccc
      241 gagcttgtcc gcaggaatgc cgacccccgt atccgccacg gtgatcacga aagcatcctc
      301 ttcaaaggcg gcgctcagcg acaccgagcc ccgttcggtg tacttgaacg cgttgccgag
      361 caggttctgg aaaatctggc tgaagaggat ccggtctgtc gatacgacgg gcgccgggcc
      421 gtcgatccgc aggcggatac ccttttcctc ggccatgggc tcgaattcgt cgcgcaattc
      481 ggcgaacaca tccttcagcc gcacgtccgt ctggagaggt gtaatggcgc ccgattccag
      541 ccggctgatg tcgagcagcg cattcagcag ctcggtcatc ctttcgatgg cctgcccctg
      601 gtgctacagg atctcctgca cgtcgggcgg cgagctcatc ttcagcatgg ctgcgttgag
      661 caggcgcagg gtctgcatcg gttggcgcag gtcatggctc gccatggcga gaaaacggct
      721 cttggcgcga ttggcccgct ccgcgacatc gcgcgcggcg ctgagtttct cgctgaactg
      781 ccgacgctcg ctgttgtcgc ggatcgctgc cgccacgtat agcctcccgt cgacccggaa
      841 cggcgagagt cggatgtcgg cggcgagctc cgtgccatca gcgcacaacg cgaggaggtc
      901 cggcacttgc gcgcccattt cccgcttgcg cgggcttctc aggaaacctg ccgtgagctg
      961 cccgtgacgc tcccgcaggc gctcggggat caggtgatcg agaggcctgc ctagaagtgc
     1021 cccgggatcg tggccgagca cctcacgcac cgtctcattg gcgaagcgga tacgcccctg
     1081 atcatcgacg acgatcacgg catcgggaga aaactggagc aggtactcgg tgattgcgtt
     1141 cgattcgccc atcagcgtcc ccggtccgcg gcgggcggcg tcaccccggc cttgccctcg
     1201 cgagacttta cagtatgcat cggatccagg cggtgcgaca tcgggatgcg gtacgggtca
     1261 tgcccaccgt gagccggcgc aaccaagggg ccgtcgcatg accctgaggg ggttgtcttg
     1321 ggtgtgtgga tcgtccactc accggcaccc ttgtacatcg acggctgtca ggatacatgt
     1381 tgggcaccgc acggctggaa tcgaggcaat tcagtgactt cagcgacgac tctccccatg
     1441 gatccggcgc cccccaacgg gaagaccgcg cgcctgctgg cacagttgat gcagatcgcg
     1501 cgcatttccg cactggagga gatggcgagc ggcttcgcac acgaactcaa ccagccgatc
     1561 ggggcaatca ccacctacgc ccaggccggc gtgcgcatgc tgagccagcc cgggccgatg
     1621 gtcgcctcgg ctatcgaagt cctgcgcctc atcagcgacg aggcgctcag cgccggccag
     1681 ggcattcacc gtattcgcgg ccagttcgac tcccggacct tcccgcgcga gacctgcgac
     1741 ctggccgacg tgctgatgga attgcggccg gtgctggacc tgctggcgat tcgcgccggc
     1801 ctcgaactgg cgttcacgct ggagccggga ctgcccagcg tgtcggtcga tcgcctgcgc
     1861 atccagcacg tgctgttcgc cctggtacag aatgccctgg aggttccgcg ccgggacagc
     1921 gcggtgcccg aagtgcggat cgaggtcacc ggggatcgat atggggtgct gaccgcggtg
     1981 atcgatggcg gcgcgggggt ttccgccgaa gcccacgaga acctgtttcg ccccttctac
     2041 acgacgaagc gcgacggcac cggactcggt ctcgcgtcga gccgggccat catcgaggcc
     2101 cacgacggca cgataggatg cgagaacgtc accggcggtg gaacgcgctt ctggttccgc
     2161 ctgccggcgg catcacggga ggacatcgca caatgactgc ggcgccgcat caggatccga
     2221 agagcaagcc gaccatttac atcgtcgacg acgacgacgg catgcgccgg gcactgaccc
     2281 tgttgatggc cacagtcggt tacaacccgg tggcgttcgc gcggccgagt gaattcctgg
     2341 cgaagtatga cccgagcctc gccggctgcc tggtgatcga cgtgcgaatg cccgagatga
     2401 gcggcctcga agtgcagcag cagctcaatc gcagcggttc gatgctcccg gtgatcctca
     2461 tcacgggcca cggcgatatc ccgatggccg tacaggcgat gaagaacggt gcctcggact
     2521 tcctgcagaa gccgtttcgc gaccaggacc tgctggacag catcaacgcc gcgctcaaac
     2581 aggatgccga gaaccggacg agtgtggaac ggctgaccga tctccggcgg cgcgtcgagt
     2641 cgctgacacc gcgcgaacag gaggtgatgg acctggtcgt cgacggccgc gccaacaagg
     2701 tgatcgcgat cgacctcggg ctgagcgagc ggaccgtcga gatccatcgc gccaacgtga
     2761 tggccaagat gggtgcccgc tccgtggccc acctcgtcaa gatgtgcctg atgctcggcg
     2821 aacccgccta gcgtcacgcc tcgagcgtcg cgcgcaggtg ttgcgccagc tcctccgcat
     2881 cgacgggctt gtggaacacg cggcaattgg ccaggtgttc gcagtgtcgc accacacgca
     2941 gcatgtcgcc gctcaggacg acggcgggga tgtcgcggcc taccgtcgcg cgcacccgtt
     3001 cgatcagctc cgcgccggtc accggcaggc cgagttgaaa gtcgctcacg atcaggtcgg
     3061 gcgcacgatc caggcgcccg agcttcgcga gggcctcgtc cggcgagccg gcggtgatga
     3121 cctcgtggcc ttccacgcgc agaaatatcg ctgtcgcctg cagcacggtc gcctcgtcat
     3181 cgacgagcag gatgccgccg ccgcgccgtg gcacgccgcc cgccactgcc gcccgcactc
     3241 cggccggctt cgattccctg ccgggcgtca cctcgatcgt gaacgaggtc cccacgccga
     3301 cggtcgattc gaccttcagc tcgatgccga gcaccttgac gagctggcgc acgactgcaa
     3361 ggccgaggcc aaagccctcg cgcgggcgcc cctgcgaccg cagctggtgg aacggctcga
     3421 aaacgagatc caggtcggcc acagggatcc ccgggcccga atccctgacc tcgatcagca
     3481 cgcggtccgc agtgcgctcg acgccgacgc gcacgccgcc gcgctcggtg tagcggaccg
     3541 cgttgcccac gaggttctgc agcacctcct tgagcagggt cgggtcggac ttcacgaccg
     3601 ggctgtccgt ggcgaattcg agcgacaagc ccctcgcgtc cgcgagcggt gcgaactcgg
     3661 cccgcagcgg cgccatgatt tcctgcaatg gaaagtcccg gatctgcggg cggacgacac
     3721 ccgactcgag catgctgata ttgagcagcg cgtggatcat gcgccccatc gagtcgatca
     3781 tctgctgctg cgcctcgacg atatcgcgca ccggctgagc gagcggctgg ctacgcaggg
     3841 tcgcattgag cagcgtcagc gagtgcaacg gctggcgcag gtcgtggctc gccgcggaga
     3901 agaaccgcga cttgctctcg cccgcctcca ccagctccgc cgtgcgttcg gtgacgcgcg
     3961 cgtcgagcag ctcgtgttcg cgctgcagct ctgcctccgc cgcgcgacgt tgcctcacct
     4021 cggcctcgag caccgcattg gctgcggata gcccttcggt tgcatcgcgt acacgcagct
     4081 caaggctggc attggccgca tcgagctgcc gcgccgcctc gacgcgcctc gcgaactcgc
     4141 ggcggaccag taaaaggatg acgaccatca gggcgatcgc catgatcgac agcagcgtgc
     4201 tgaaggcaag gttgcgcccg agactcgtct gcgagctcgc catgcgcgca tccagttgcc
     4261 gcagctccac cgccgacatg cggtcgagga gctggcggat cgcgtccatg acgaaatgcc
     4321 cctggttcgt ggtcacgaag gccagcgcgg catccaggcc ctgggcgcgt tgcagatcga
     4381 gcacctggtc catctgcttg cggcgcgtat cgagcagccg ttcgagcgcg cggaagttct
     4441 cgagctgacc gctgtcgccg tcttcggcga gcaaccgttg gagaacgggc aggcgcgcgg
     4501 cgagctgcac ctggcctgcg atgtacggct cgatgtactc cccgcgcgag gtcagcagat
     4561 acccgcgctg gcccgtttcc atgccgagta tcgcttcgct gatggtttcg agcgtgaggc
     4621 gccgctcctg ggtgcgttgc agctgctcga aggtcacgcg cacctgctgc acgcagtagt
     4681 actcgagcgc gagcacgacg ccgagcagga cggcgccgca cagcagcccg atcagggcct
     4741 ggctgtcggg agacaggcgg cgaggcgaca tccggtccgg ctcttcaggg gacttccggc
     4801 tggtcgcgca ccagcagcac gggcaccggc gaggagcgta cgaccaattc cgcatcgctg
     4861 cccatcgcca ggcggcgcag accgcgacgg ccgtgcgtgc ccatgacgat cagatccgcg
     4921 gccgcgagca ctttccgtcc gccttcgcgc tgcgactcga acagctgctc gtagaaaggc
     4981 gcgggcatgt agcccgtgtc gaagacctgt tcgttcacga cgtgaacgat atcgagcatc
     5041 gctccgcaag acttcgccag accgactgcc tcgttgagtc cgcgcagtga tgtcgcgctg
     5101 ccgtcgaccg gcacgagtat ctttttgtac attgccgcga tctccaggga cgactggcac
     5161 cgatcctagg ggctgccccg gcgcacgcca gccgggagtt ccccgcaccg cgcacgcact
     5221 tccacggccg gcgccgctag ccggcagcgg cctgctgcag ccgcaggtgc tgcgcggcca
     5281 ggtccaggac cccccgtgcg tgcacctggg caaccgctcg cgccgcggtc ggcaggctcg
     5341 ccggggcgca ctcgaccgcc gcggcgaccc gccccggcgt gccgagatcg ctccagccgc
     5401 aggacggcac ggccagcacg cgcagccgcg gcgtgcagcg ctcgacaata tcgcgcgaga
     5461 aatcgagcac cggcagccgc tcgtacagtt ctgcgaggcg tgcctgtcgc aacgcttcgt
     5521 cgttcgtggc caccacgatg gcgcgcatct cgccgacgac ctccgggcag cgttcttcga
     5581 atgcacccag cagcgcccgg ccatgcgcgg cgaagatgaa cgagttccac aacccgcccc
     5641 gttcgatgag ggcttcggcc gtcttcccgc tgggcttctc gacgaactgg cagacccccc
     5701 gcgcacccgg tccgcgatcg tccgacggca cgatgtaacc caactccgcg tccgcctgct
     5761 cgggcgcgaa ccccagcagt acgatgaggc cgcgctgccg ctcggtctcc ttcatgagct
     5821 gctcgaggct cgcggcgagc accgcttcat cgcgaacata atggtccgac ggcaggacga
     5881 ggagcgaagc ctccggatcg cggtgcagga tctgcaacaa aggcaggagg atgccgttcg
     5941 ccgtgccgcg attgcgtggc tggacgacga tgttccgcgc ctcgatcgac cgcggcagcg
     6001 tccgccacca ccgttcgtga tgggcggcga cgatgacaca ggtgcgcgct ggcggcgcga
     6061 tcactcgcgc ccgttgcagc gctgcgttca gcaacgagcc tccgccgccg agcgaacaga
     6121 actgcttggg aacggaaatg ccggcggcga tcgtcgtgag tgcctgcagg cgggcaccat
     6181 cccccgcggc gagtacgagc gcccagcgat gcccggcagc cggcgaaggt gacatcaata
     6241 cccccttccg ggtaaccgga caggggcaca gggccggccc cgatctgcgg ccccgtactc
     6301 cggcatgatc gtgttcatcg ctgcgctccc cgccactggc ggacgcggtg atgctaattg
     6361 gacgacggcg gcagctttac ccggaattcc ccgcagccgg cacgcggttt cgcgggtaca
     6421 cggcgcgtgg gatcgcctgg tcgacgggag ggcccgcgtg agttcgcacg gccgcaccgc
     6481 tgcagatggc gtccccaagg ggattcgaac ccctgtcgcc gccgtgaaag ggcagtgtcc
     6541 tgggcctcta gacgatgggg acgccgtgaa cgaaatttgg tggagctaga cgggatcgaa
     6601 ccgtcgacct cttgcatgcc atgcaagcgc t
//
LOCUS       DAOQVY010000130         6606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_12798_length_6606_cov_5.628759, whole genome shotgun sequence.
ACCESSION   DAOQVY010000130 DAOQVY010000000
VERSION     DAOQVY010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12798_length_6606_cov_5.628759"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2314
                     /gene="dnaE"
                     /locus_tag="PKN91_10755"
     CDS             <1..2314
                     /gene="dnaE"
                     /locus_tag="PKN91_10755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNS28587.1"
                     /translation="FCMDGRDRVIDYVANHYGRERVSQIITYGTMAAKAVVRDVGRVL
                     GMSYGHVDRIAKLIPFELGITLEDALKKEPELRRLYESDEEITNLIDLARSLEGLTRS
                     AGTHAGGVVIAPSVLTDFAPLYCEEGSQSVVTQFDKDDVEAAGLVKFDFLGLRTLTII
                     DRAITTINALRASAGEPPIAIADLPMDDAPTFALLKSCRTTAVFQLESRGMKDLIRRL
                     QPDRFEDIIALVALFRPGPLQSGMVDDFIARKHSPEGTTIDYLHPELQPVLAPTYGVI
                     LYQEQVMQIAQVLAGYTLGGADLLRRAMGKKKPEEMAQQRSVFVEGATARGVNATQAA
                     HIFDLMEKFAGYGFNKSHSAAYALLSYQTAWLKAHYAAAFMAAVLSADMDHTDKVVTL
                     IDECAQIGIDILAPHVNHSMHAFTVAGERAIRYGLGAIKGVGEGAVEALISEREAHGP
                     FASLEDLCRRLDLTRINRRALEALVRSGSLDGLGANRATMLGRIDAAVQLGEQEARAR
                     EVGQADFFGLDAAANADPVVQSVPEQPESSESVRLAGERETLGLYLTGHPIDRFLTGL
                     PHFVSHRIGDLVSDRPVGGPEGGRGFRGKPATVAGLVTEVRRRGTRVAIVLDDRSGRI
                     EADFFEDVFQQYRELIAKDALLVVEGQVRFDEFSDGWRLAARRVTELDRVREQVARRI
                     VIRCPADSPARASLPDALAGLLTPWRPGPCQVSIDYEGTGARGVLTLGSEWNVRAARE
                     LLEQLEMLAGPGNLRVLYGPPPGVDASMTG"
     gene            complement(2272..3795)
                     /locus_tag="PKN91_10760"
     CDS             complement(2272..3795)
                     /locus_tag="PKN91_10760"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNS28588.1"
                     /translation="MLRRDFVRAATALATLAPASGAIARTLALLATAPNLLPARSLSG
                     GELQLPKSDVEALAASLRGELLFPGADGYDAARRVWNGSFDKRPALIARCTGNADVMA
                     AVDFAREHELLTAVRGGGHSTSGKSTCDGGIVIDLSAMRGATVDPRARRARVRGGALL
                     GDLDHETRPFGLVTTAGTVSHTGAGGLTLGGGFGRLCRHFGLACDNMLAAEIVTAAGE
                     LVRASAGENPDLYWALRGGGGNFGVVTAFEYQLHPMDPIVFGGVMLWPAARAGEIFRR
                     FRDFSLQAPDALNVDIFLVDRGDSPVIGIELCWSGARAQGERVVAPLRAIGGTLLDTV
                     APVPYLAMQTSGDAQFHGGVNFYGKAGFLVALEDSTIDALVEGFEAAAPGSAGVFIQQ
                     SGGEVGRVAPTATAFVNRDSRYWLMVNSVWKDPADREGHIASARALWARVEPAVSGFY
                     INTMADEQYARARQVFGVNYDRLTRVKGRYDPHNLFRLNANIPPQPVMEASTPGGGP"
     gene            3913..4878
                     /locus_tag="PKN91_10765"
     CDS             3913..4878
                     /locus_tag="PKN91_10765"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNS28589.1"
                     /translation="MAVSFLDFEQPIAELEAKIEELRHVTSESRVSIKDEIGKLQAKS
                     RQLTKSIFANLTPWQITQLARHPQRPYTLDYIAAIFTDFQELHGDRMYGDDLAIVGGL
                     ARLEGRPLMIIGHQKGRDTKERVRRNYGMPKPEGYRKALRLMRLAERFRLPLVTFIDT
                     TGAYPGVGSEERGQSEAIARNLFEMSVLGIPIVSVVIGEGGSGGALAIGVCDRLLMLQ
                     YATYSVISPEGCASILWKNSDKKEIAAEAMGLTAARLAELNLVDAVLPEPLGGAHRDP
                     QAMYATMRAALLEQLAALDQMSPEQLRDARNARIAGFGVYSEAAS"
     gene            4890..6305
                     /gene="tilS"
                     /locus_tag="PKN91_10770"
     CDS             4890..6305
                     /gene="tilS"
                     /locus_tag="PKN91_10770"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNS28590.1"
                     /translation="MAAFGPDILEAQLGALLPDFPRVALVAAFSGGGDSLALLAALAA
                     LRRRHRRLALRAVHVDHGLQDSSRAWVRACRLYARELRVPIVVRRVTVDCPKGQSLEA
                     AARVARHAAFASLLRADEALLTAHHLEDQAETVLLQLLRGAGIAGLAAMPARAPLGKG
                     WMLRPLLDVPRASLREYARQRGFDWIEDPMNADRHFDRAYVRHELLAPLQARWPAAAR
                     SLARTARHAADARQLLAGIAAKDLLAAMDGPDLSVPVLRRLPDDRRRNALRAWIVQHG
                     SRVPETRRLAEIAGPMLAARADAQPRVAWDEGGVRRHGNRLVWEPAGAARGAKMPVEA
                     AQGGSAAVEDWRWQDIPVIELPGARGRIGIRADEHGALDLDLLPPGLAVRWRLGGERL
                     RPRRGGPRRSVKRLLQVAGIAPWERAAVPLVYAADRLVAVGERWIDADFQVSAATRRR
                     GRIVWQQSPRRARPGGANLLT"
     gene            6372..>6606
                     /gene="pyrG"
                     /locus_tag="PKN91_10775"
     CDS             6372..>6606
                     /gene="pyrG"
                     /locus_tag="PKN91_10775"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187546.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HNS28591.1"
                     /translation="MARFVFFTGGVVSSLGKGIAAASLGAILESRGLTVAMVKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERF"
BASE COUNT         1004 a         2231 c         2354 g         1017 t
ORIGIN      
        1 cttctgcatg gacggccgtg accgcgtcat cgattacgtc gccaatcact acgggcgcga
       61 gcgcgtctcg cagatcatca cctacggcac gatggcggcg aaggccgtcg tgcgcgacgt
      121 cgggcgcgtg ctcggcatga gctatgggca cgtcgaccgg atcgccaagc tgatcccgtt
      181 cgagctgggc atcacgctcg aggatgcgtt gaagaaggag ccggaactgc ggcggctcta
      241 tgagagcgac gaggagatca ccaacctgat agacctcgcg cgctcgctcg aggggctgac
      301 gcgcagcgcc ggcacgcacg cgggcggcgt ggtgatcgcg ccgtcggtgc tgacggattt
      361 cgcgccgctc tactgcgagg aaggctcgca gagcgtcgtc acgcagttcg acaaggacga
      421 cgtcgaggcg gccggcctcg tgaagttcga cttcctcggc ctgcgcacgc tcacgatcat
      481 cgatcgcgcg atcaccacca tcaacgcgct gcgcgcgtcc gcgggcgagc cgccgatcgc
      541 gatcgccgac ctgccgatgg acgacgcgcc gaccttcgcg ctgctgaagt cgtgccgcac
      601 gaccgccgtc ttccagctcg agtcgcgcgg catgaaggac ctgatccgcc gcctgcagcc
      661 cgaccgcttc gaggacatca ttgcgctcgt cgcactgttt cgccccggac cgctgcaatc
      721 gggaatggtc gacgacttca tcgcgcgcaa gcacagccca gagggcacga cgatcgacta
      781 cctgcatccg gagctgcagc cggtgctcgc gccgacctac ggggtgatcc tgtaccagga
      841 acaggtgatg cagatcgcgc aggtgctggc cggctacacg ctcgggggcg ccgacctgct
      901 gcgtcgggcg atgggcaaga agaagcccga ggagatggcc cagcagcgct cggtattcgt
      961 cgagggcgcg accgcccgcg gcgtcaatgc cacccaggcc gcgcacatct tcgacctgat
     1021 ggagaagttc gcgggttacg gcttcaacaa atcgcactcc gccgcctatg cactgctgtc
     1081 ctaccagacg gcgtggctca aagcgcatta tgcggcggcc ttcatggccg cggtgctgtc
     1141 ggcggacatg gaccataccg acaaggtggt cacgctgatc gacgagtgcg cccagatcgg
     1201 gatcgatatc ctcgcgccac acgtcaacca ctcgatgcat gccttcaccg tcgccggcga
     1261 gcgcgcgatc cgctacgggc tcggtgcgat caagggtgtc ggcgagggcg cggtcgaggc
     1321 gctgatcagc gagcgcgagg cgcacggtcc gttcgcgagc ctcgaggacc tgtgccggcg
     1381 cctcgacctc acgcggatca accgccgcgc gctcgaggcg ctggtacgct cgggcagcct
     1441 cgacgggctt ggcgccaacc gcgcgacgat gctgggccgg atcgatgccg cggtacagct
     1501 cggcgagcag gaggcgcgcg ccagggaggt gggccaggcc gatttcttcg gcctggacgc
     1561 ggcggcgaac gccgatcccg tggtgcagtc ggtgccggag cagcccgaga gcagcgagtc
     1621 ggtgcgcctc gccggcgagc gtgaaacgct gggcctgtat ctcaccggac atccgatcga
     1681 ccgcttcctg acgggcctgc cgcacttcgt gagccatcgc atcggcgacc tggtctcgga
     1741 ccgcccggtg ggcggaccag aggggggcag gggttttcgc ggcaaaccgg ccaccgttgc
     1801 gggcctcgtc accgaggtgc gccggcgcgg cacccgcgtg gcgatcgtgc tcgacgaccg
     1861 ctccggacgc atcgaagcgg acttcttcga ggatgtgttc cagcagtacc gcgagctgat
     1921 cgccaaggat gcgctgctgg tcgtcgaggg ccaggtgcgc ttcgatgaat tcagcgatgg
     1981 ctggcggctc gccgcgcggc gcgtcaccga actcgatcgc gtgcgcgagc aggtggcgcg
     2041 acgcatcgtg atccgctgtc ccgccgactc gccggcgcgg gcgagcctgc ccgatgcatt
     2101 ggccgggttg ttgacgccgt ggcgccccgg gccctgccag gtctcgatcg actacgaagg
     2161 caccggcgcg cgcggcgtgc tgaccctcgg cagcgagtgg aacgtgcggg cggcgcgcga
     2221 gctcctcgag cagctcgaga tgctggcggg tcccggcaac ctgcgggtgc tttacggccc
     2281 gccgccgggc gtcgacgcct ccatgacggg ctgaggcggg atattggcgt tcagccggaa
     2341 caggttgtgc gggtcgtacc ggcccttcac cctggtgagc cggtcgtagt tgacgccgaa
     2401 gacctggcgc gcccgcgcgt actgctcgtc ggccatggtg ttgatgtaga agccggacac
     2461 cgcgggctcg acgcgtgccc acagtgcgcg tgccgaggca atatggccct cgcggtcggc
     2521 ggggtccttc cagaccgagt tgaccatcag ccagtagcgc gagtcgcgat tgacgaaggc
     2581 ggtagcggtg ggcgcgacgc gaccgacctc gccgccgctt tgctggatga acacgccggc
     2641 acttcccggg gcggcggcct cgaagccctc gacgagtgca tcgatcgtcg agtcctcgag
     2701 cgccacgaga aagcccgcct tgccgtagaa attgacgccg ccgtggaact gcgcatcgcc
     2761 cgaggtctgc atcgcgaggt agggcacggg ggcgaccgtg tcgagcagcg tgccgccgat
     2821 cgcgcgcagc ggcgcaacga cccgttcgcc ctgtgcgcgc gcgccggacc agcacagttc
     2881 gatgccgatg acgggcgagt cgcctctgtc cacgaggaag atgtcgacgt tgagcgcatc
     2941 gggcgcctgc agcgaaaagt cccggaaacg gcggaagatc tcgccggcgc gcgcggcggg
     3001 ccagagcatg acgccgccga acacgatcgg gtccatcggg tgcagctggt actcgaacgc
     3061 cgtgaccacg ccgaaattgc cgccgccgcc gcgcagcgcc caatacaggt cgggattctc
     3121 gccggcactc gcgcgtacga gttcgcccgc ggcagtgacg atttccgcgg cgagcatatt
     3181 gtcgcaggcg agcccgaagt ggcggcacag gcgcccgaag ccgccgccga gggtgagtcc
     3241 gccggcgccg gtgtgcgaaa cggtgccggc ggtggtgacg agcccgaagg gccgcgtctc
     3301 gtggtcgaga tcaccgagca acgcgccacc gcgcacgcgc gcgcggcggg cgcgcgggtc
     3361 gacggtcgcg ccgcgcatgg cggacagatc gatgacgatc ccgccgtcgc aggtcgactt
     3421 gccggaggtg ctgtggccgc cgccgcgtac cgcggtgagc agctcgtgct cgcgcgcaaa
     3481 gtccacggcg gccatgacgt cggcattgcc cgtgcagcgt gcgatcaacg ccggacgctt
     3541 gtcgaacgaa ccattccaga ccctgcgggc cgcgtcgtag ccgtcggcgc cgggaaacag
     3601 cagttcgcca cgcaggctcg cggcgagtgc ctcgacgtcg gatttcggca gctgcagttc
     3661 gccgcccgag agggaacgcg ccggcaacag attcggggcg gtggcgagca gcgccagggt
     3721 cctggcgatg gcgccggacg ccggtgccag cgtggcaagc gcggtggcgg cgcggacgaa
     3781 atccctgcgt aacactggcg gcttcctgct gcctgtcgga tcagccgggg agtctacgcc
     3841 gagtgcaccc gccttgcgta gccggccccc cgcccttaac ctcgcccgct tctggtcgaa
     3901 cccgtgggac ccatggccgt aagttttctc gatttcgaac aacccatcgc cgagctcgaa
     3961 gccaagatcg aggagctgcg gcatgtcact tccgaatcca gggtcagcat caaggacgag
     4021 atcggcaagc tgcaggccaa gagtcgccag ctcaccaaga gcattttcgc gaacctgacg
     4081 ccctggcaga tcacgcagct cgcgcggcat ccgcaacgcc cgtacacgct cgactacatc
     4141 gccgcgatct tcaccgactt ccaggagttg catggcgacc gcatgtacgg tgacgatctc
     4201 gccatcgtcg gcgggctcgc gcgcctcgaa gggcggccgc tcatgatcat cgggcaccag
     4261 aaagggcgcg acaccaagga gcgcgtgcgt cgcaactacg gcatgccgaa gcccgagggc
     4321 tatcgcaagg cattgcgact gatgcgcctc gcggagcgct tccgcctgcc actggtcacc
     4381 ttcatcgaca cgacgggcgc gtatccgggc gtcggctccg aggagcgcgg gcagagcgaa
     4441 gcgatcgcgc gcaacctgtt cgaaatgtcg gtgctcggca tcccgatcgt cagtgtcgtc
     4501 atcggcgagg gtggctcggg gggtgcgctc gcaatcggcg tctgcgaccg actgctgatg
     4561 ctgcagtacg cgacctattc ggtgatttcg ccggagggct gcgcctcgat cctgtggaag
     4621 aattccgaca agaaggaaat cgccgccgag gcgatgggac tgacggccgc gcggctcgcc
     4681 gagctgaacc tcgtcgatgc ggtcctgccc gagccgctcg gcggcgcgca tcgcgatccg
     4741 caggcgatgt acgcaaccat gcgcgccgcg ctgctcgagc agctcgccgc gctcgaccag
     4801 atgtcgcccg agcaattgcg ggatgcccgc aatgcgcgta tcgccggctt cggcgtgtac
     4861 tccgaagccg cgagctagcc ttgcgcaccg tggccgcatt cggcccggac atcctcgagg
     4921 cgcagctcgg cgcgctgctg cctgattttc cacgggtggc gctggttgcg gcgttctcgg
     4981 gcggtggaga ttccctggcc ctgctcgcgg cactcgccgc gctgcggcgc cggcaccgta
     5041 ggctcgcgct gcgcgccgtc cacgtcgatc acggcttgca ggacagctcg cgcgcgtggg
     5101 tgcgggcgtg ccgcctgtac gcacgcgagt tgcgcgtgcc aatcgttgtg cggcgggtga
     5161 cagtagactg cccgaagggc cagtcgctcg aggcggcggc acgcgtggcg cgccatgcgg
     5221 cgttcgccag cctgttgcgc gcggacgaag cactgctcac tgcgcatcac ctggaggatc
     5281 aggcggaaac ggtgctgctg cagctgctgc gcggggcggg cattgccggt ctcgccgcga
     5341 tgccggcgcg cgcgccgctc ggcaagggct ggatgctgcg tccgttgctc gacgtgccac
     5401 gcgcgtcgct gcgcgagtac gcacggcagc gcggcttcga ctggatcgag gatccgatga
     5461 atgccgaccg gcacttcgat cgcgcctatg tgcgtcacga gttgctggcg ccgctgcagg
     5521 cgcgctggcc cgcggcggcc cgttcgctcg cacgcacggc gcggcacgcg gcggatgcgc
     5581 ggcagttgct cgcgggcatc gcggcaaagg acctgctggc ggccatggac ggacccgatc
     5641 tgtccgttcc cgttctgcgc cggttgccgg acgatcgccg acgcaatgcg ctgcgtgcat
     5701 ggatcgtgca gcacggtagc cgcgtgcccg agacgcgccg gctcgccgag atcgcgggcc
     5761 ccatgctcgc tgcgcgcgcc gatgcgcaac cccgggtcgc atgggacgag ggcggcgtgc
     5821 gtcgccacgg caaccggctc gtgtgggaac cggctggtgc ggctcgcggg gcgaagatgc
     5881 cggtcgaggc ggcgcagggc gggtcggcag cggtcgagga ctggcgctgg caggacattc
     5941 cggtcatcga gctgcccggt gcacgtgggc gcatcggcat ccgcgccgac gagcacggtg
     6001 cgctcgacct cgatctgctg ccgcccgggc ttgccgtgcg ctggaggctg ggcggtgagc
     6061 gtctgcgtcc gcgccgcggc gggccgcgac gcagcgtcaa gcgcctgctg caggtcgccg
     6121 gtatcgcgcc gtgggagcgc gccgcagtgc cgctcgtcta cgcggctgat cgcctggtgg
     6181 cggtcggtga gcgctggatc gatgcggatt ttcaggtgag tgccgccacg cgcaggcgcg
     6241 gtcgcatcgt gtggcaacaa tcgccgcgcc gcgctcgccc gggcggcgcc aatttgctaa
     6301 cctagctgcc cgtcgtgatg cacccccgcc gggggtgagc ggcaccggtt ctttttcgcg
     6361 gcggacacct catggcccga ttcgttttct ttactggcgg cgtggtttcc tcattgggga
     6421 agggcatcgc cgccgcatcg ctgggcgcca tactcgagtc gcgcggcctc accgtggcga
     6481 tggtcaagct cgatccgtac atcaacgtgg atccgggcac gatgagcccg ttccagcacg
     6541 gcgaggtgtt cgtcaccgag gacggggcgg agaccgacct cgatctcggc cactacgagc
     6601 gcttca
//
LOCUS       DAOQVY010000131         6508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_13049_length_6508_cov_4.915853, whole genome shotgun sequence.
ACCESSION   DAOQVY010000131 DAOQVY010000000
VERSION     DAOQVY010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13049_length_6508_cov_4.915853"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /gene="nadE"
                     /locus_tag="PKN91_10780"
     CDS             complement(<1..732)
                     /gene="nadE"
                     /locus_tag="PKN91_10780"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002048.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNS28592.1"
                     /translation="MMKSQPLSADRAFALDHATEIERIAGKLRHALTHDLNRRGLVVA
                     ISGGIDSSVSAALAVRAVGPKRVYGLLLPERVSSPQSTHRGRLLAEHLGIEYEIFDIA
                     PALEALGCYARIDEVCKAVHQGYGAGWKNKIVLVGGLEGRYNHFKLVAQDPSGQIHEA
                     RLEYKQYLQIVAATNFKQRVRKTMEYFHADRLNYAVVGTPNRLEYDQGFFVKNGDGSA
                     DVKPIAHLYKSQVYALARFMKLPDEI"
     gene            complement(729..2270)
                     /locus_tag="PKN91_10785"
     CDS             complement(729..2270)
                     /locus_tag="PKN91_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNS28593.1"
                     /translation="MPAGPTSLVDLLRASAQRHPGRQAIRHRGAEIHYGDLWREVRRA
                     ANALRAGGLQPGDRVALLIENSIDYVIAYYAALQAGGVAVGLNTAMRGPELARNVAHC
                     AATALIAMPGHPELPGVIAACGTLRQVVTTPATGPARKAPDGAVAWHEWMAAADDADV
                     PETIEPQAPASIIYTSGTTGDPRGVTLSHRNLVANTLSILEYLGLTEADRIVNVLPFY
                     YSYGNSVLHTHLAAGGSLVLENSLTYPHLVVQAMAEQRATGFSGVPSTFALLLQRVKF
                     ADYDLSSLRYLTQAGGAMAPVLTERLLAALPGARLFVMYGQTEATARISYLPPERLAS
                     KLGTVGIGIPGVTIEVQDEAGRAVPAGTAGEVCVRGDNVMLGYWENPAATAKVLHDGW
                     LHTGDIGQLDADGYLTLVGRSSDMIKTGAHRVSPLEIEEVVLELAGVAECAAVGVADP
                     ILGEVVRVCVVAADGAAPEARAVQAHCRDRLAAYKVPKEVVFVATLPKTASGKVKRFM
                     LKEAG"
     gene            complement(2275..2520)
                     /locus_tag="PKN91_10790"
     CDS             complement(2275..2520)
                     /locus_tag="PKN91_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNS28594.1"
                     /translation="MSVAEQVRQYILENFLFSTDVSLLGLDESFLERGLIDSTGMLEV
                     ILFLEETFGVKVADDEMVPENLDSVNRIAAFVERKKA"
     gene            complement(2554..4497)
                     /gene="asnB"
                     /locus_tag="PKN91_10795"
     CDS             complement(2554..4497)
                     /gene="asnB"
                     /locus_tag="PKN91_10795"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNS28595.1"
                     /translation="MCGIAGILNLAPGAPVTGTELAGMLQPLYSRGPDGQGTYLDEPQ
                     LVGLAHARLSIIDLAGGAQPIHNEDRTVWVTFNGEIFNYVELRAELEQAGHRFYTHTD
                     TEVIIHLYEEHGLRFAERLNGQFAIGLWDVRARRLVLARDRAGILPLFYSRDGGRLVF
                     ASEIKSLLRGLSHRPQMNRVALDQVFTFWSPVGATTLFEGIEQLQPGHMLVAENGRVS
                     ISQYWNWIFREPGDYLKGSVEDLARELHGLLADATRIRLRADVPVGAYLSGGLDSTAL
                     VALMCEGGVKPNTFSLAFEDPGLDESEHQLAAAGHFGVNHRTIRVGDEHIRDGFVRTV
                     WRAETPFLRCAPIPLMALSELAHRSDCKVVMTGEGADEVFGGYDLFKEAKIRRFWARR
                     PDSKWRARLLQRLYPYLDITSARGQAYSEAFFGSGLAGKESAFFAHGPRWKTTAQGKV
                     LFSEETRERIDGAAAVAEFAHALPAGFGAWDPFNQSQYVEAKTLLAGYLLSTQGDRML
                     MANSVEGRFPYLDHRVIEFANALDPRLKMRVLNEKYLLKKALGPQLPPGIAARKKQPY
                     RSPDSKAFEAGRVEFVEDALSEASLKRTGYFDAGRVQLLWRKVKSGRAVSYKDSMTFI
                     GVLSTQVWHGLFVDGVTPPGGRL"
     gene            complement(4516..>6508)
                     /locus_tag="PKN91_10800"
     CDS             complement(4516..>6508)
                     /locus_tag="PKN91_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNS28596.1"
                     /translation="ARLAALRAEGIVLLAAMFMVVGAVGVWVKLAQLSQSTGYLAISR
                     FLGYSHESQFAWWQKLSFLRLDLMIQGFALPVGLALLLRALPRALRVPSAAVVAILLC
                     TFYYFQLQSLGSIGRYLTPDLIQDAIFWAIDFPEAIGEYASPRGALKLLAAVTVIVVL
                     AVAAARRSRGVGAGGLVRTIEIGTLAALAGSLVLALIAAAAPVPSLAPHRSALRTMVQ
                     AMLPQGGAYDIPRHASREELLALFRRAARQTEGSSPAVTAGLDAGHDVILFIMETGPA
                     RSYDPIGDRDSLPGVSMLLDRAFVGRHHLTTYPYTSDAIFSILTGMYPIGRGPFLRKA
                     QSLSGAGLVPPLAARGYSGAVYLPYPDSFENDTAMYAISGVSKTYVADPKEAQRFAQA
                     AQRAQRTIEALPADSPARRERAALLADRLTHDLMALDAMLTDIVASKQAGERFVTLFV
                     PQIGHAPWFDLYGRESVVERGRDVMILQDAWLAEIVTRLAEGGWLDDTVIVVTADHGV
                     RTRVEDPGLPSGTITDYSFEVPLVIFAPGTLTAMREVTARTSHVDIAPTVHALLGAGG
                     DGTLSQGVPIWDEAALAERRVFMFAAGYLGADAYAEDGRYVVRRWLNGFVHESTTLPV
                     GAGTMVTQPEAASRLAEPIDLMYALQPALLEVLHSDAPP"
BASE COUNT         1124 a         2227 c         2136 g         1021 t
ORIGIN      
        1 gatctcgtcc ggcagcttca tgaagcgtgc cagcgcatag acctgcgact tgtagaggtg
       61 cgcgatcggc ttcacgtcgg ccgagccgtc gccgttcttg acgaaaaagc cctggtcgta
      121 ctcgagccgg ttcggcgtac cgacgaccgc gtagttcagg cggtcggcgt ggaagtactc
      181 catcgtcttc ctgacgcgct gcttgaagtt ggtcgcagcc acgatctgca ggtactgctt
      241 gtactcgagc cgcgcctcgt ggatctgccc cgacgggtcc tgtgcgacga gcttgaagtg
      301 gttgtagcgg ccctcgaggc cgcccacgag cacgatcttg ttcttccagc ccgcgccgta
      361 gccctggtgc accgccttgc acacctcatc gatgcgcgca tagcagccga gcgcctcgag
      421 cgccggcgcg atgtcgaata tttcgtactc gatgccgagg tgctcggcga gcaggcggcc
      481 gcgatgcgtg ctctgcggcg aggacacgcg ctccggcagc agcaggccgt agacccgctt
      541 cggcccgacc gcgcgcaccg cgagcgccgc gctcaccgag ctgtcgatgc cgcccgagat
      601 cgcgaccacg agaccgcggc ggttcaggtc gtgcgtgagc gcgtggcgca gcttgccggc
      661 gatgcgttcg atctcggtgg catggtcgag ggcgaaggcc cgatcggccg acaacggctg
      721 tgacttcatc atcctgcttc cttcagcatg aaacgtttga cttttcccga ggcggtcttc
      781 ggcaaggttg ccacgaaaac cacttccttc ggtaccttgt atgcggcgag ccggtcgcga
      841 cagtgggcct gcacggcacg cgcttcgggc gcggcgccgt cggccgcgac gacgcacacg
      901 cgcacgacct cgccgaggat cgggtcggcg acaccgaccg cggcacactc ggccaccccc
      961 gcgagttcga gcacgacttc ctcgatctcg agcgggctca cccggtgggc gccggtcttg
     1021 atcatgtccg acgagcgccc gacgagcgtc aggtaaccgt cggcatcgag ctggccgatg
     1081 tcgccggtgt gcagccagcc atcgtgcagc accttcgccg tggccgccgg attctcccaa
     1141 tagccgagca tcacattgtc gccgcgcacg cagacctcgc ccgcggtgcc cgcgggcacg
     1201 gcgcggccag cctcgtcctg cacctcgatc gtgacgcccg ggatgccgat gccgacagta
     1261 ccgagcttcg aggccagccg ctccggcggc aggtagctga tgcgcgcggt cgcctcggtc
     1321 tggccgtaca tgacgaacag acgtgcgccg ggcagcgctg ccagcaggcg ctcggtgagc
     1381 accggggcca tcgccccgcc cgcctgcgtc agatagcgca gcgacgacag gtcgtagtcg
     1441 gcgaatttca cccgctgcag caacagcgcg aaagtcgaag gcacgccgga gaagccggtc
     1501 gcacgctgct ccgccatcgc ctgcacgacg agatgcgggt aggtcagcga gttctcgagc
     1561 accagcgagc cgcccgccgc gagatgggtg tgcagcaccg agttgccgta cgaatagtag
     1621 aagggcagca cattgacgat gcggtcggcc tcggtgagcc cgaggtattc gaggatggac
     1681 agcgtgttgg caacgaggtt gcgatggctc agcgtgacgc cacgaggatc gccggtcgta
     1741 cccgaggtgt agatgatcga tgccggcgcc tgcggctcga tggtttcggg gacatcggcg
     1801 tcgtccgctg ccgccatcca ctcgtgccag gccaccgcgc cgtccggcgc cttgcgtgcg
     1861 ggacccgtcg cgggcgtcgt caccacctgg cgaagcgtgc cgcaggcggc gatcacgccc
     1921 ggcagctccg gatggcccgg catggcgatc agcgccgtcg ccgcgcaatg cgcgacatta
     1981 cgcgcgagtt cgggtccgcg catcgcggta ttgaggccga ccgcgacgcc gcccgcctgc
     2041 agcgcggcgt agtaggcgat gacgtagtcg atggagttct cgatcaacag cgccacacgg
     2101 tcgcccggct gcagcccgcc ggcgcgcaat gcattggccg cgcgccgcac ctcgcgccac
     2161 aggtcgccgt aatggatttc ggcgccgcga tgccggatcg cctgccggcc cgggtggcgc
     2221 tgcgccgacg cgcgcagcag gtccaccagg ctggttggac ccgccggcat ttcctcaggc
     2281 cttcttgcgt tcgacgaacg cggcgatcct gttaaccgaa tcgagattct cgggaaccat
     2341 ctcgtcgtcg gccaccttga cgccgaaggt ctcctccagg aagaggatca cctcgagcat
     2401 gccggtcgag tcgatcagcc cgcgctcgag gaaagactcg tccaggccca gcagcgacac
     2461 atcggtgctg aagaggaaat tctcgagaat gtactgcctg acctgttcgg ctaccgacat
     2521 cgggcgctcc actgtttggg accgggaatt tgactataga cgaccgcccg ggggagtgac
     2581 gccatccaca aaaagaccgt gccacacctg ggtcgacagc acaccaatga aggtcatgct
     2641 gtccttgtag cttaccgcac gaccgctctt tacttttcgc cacagcagct gcacgcggcc
     2701 ggcgtcgaag tagcccgtgc gtttcaggct cgcctcggac agtgcgtctt cgacgaattc
     2761 gactctgccg gcctcgaacg ccttgctgtc ggggctccgg tagggctgct tcttgcgcgc
     2821 ggcgatcccg ggtggcagct gcgggccaag cgccttcttc aacaggtact tctcgttcag
     2881 tacacgcatc ttcaaccgcg gatcaagcgc gtttgcgaac tcgatcacac gatggtccag
     2941 atagggaaag cgcccctcca cgctgtttgc catcagcatg cggtccccct gggtcgacag
     3001 caggtagccc gcaagcaagg ttttcgcctc gacgtactgc gactggttga acggatccca
     3061 ggcgccgaaa ccggccggca acgcgtgcgc gaactcggcg accgcagccg ccccgtcgat
     3121 tcgctcgcgc gtttcttccg agaacagcac cttgccctgc gcggttgtct tccagcgcgg
     3181 cccgtgcgca aagaatgcac tctcctttcc ggcgagaccc gaaccgaaga acgcctcgct
     3241 gtacgcctgt ccacgagccg aggtgatgtc gagataggga tagagccgct gcagcaggcg
     3301 cgcgcgccac ttcgaatctg gtcggcgcgc ccagaagcga cggatcttcg cctccttgaa
     3361 caggtcgtaa ccgccgaaga cctcatcggc gccctcgccc gtcattacca ccttgcagtc
     3421 gctgcggtgc gcgagctccg aaagcgccat cagcggaatc ggggcgcagc gcaggaacgg
     3481 cgtctcggca cgccacaccg tgcgcacgaa gccgtcgcgg atatgttcgt cgccgacgcg
     3541 gatcgtccga tggttgacgc cgaaatgccc cgcggccgcc agttggtgct cgctttcgtc
     3601 gagacccgga tcctcgaagg cgagcgagaa agtgttcggc ttcacgcccc cttcgcacat
     3661 caacgcgacc agcgccgtcg aatccagccc gcccgaaagg taggcgccga ccggcacgtc
     3721 ggcacgcagc cggatgcggg tcgcatcggc gagcagcccg tgcagttcgc gcgcgagatc
     3781 ctcgacgctg cccttcaggt aatcgcccgg ctcgcggaaa atccagttcc agtattgcga
     3841 gatcgacacg cgaccgttct cggcgaccag catgtgtccc ggctgcagct gctcaatgcc
     3901 ctcgaacagc gtggtggcgc cgacgggcga ccagaatgtg aacacctgat cgagcgcaac
     3961 gcgattcatc tgcggccggt gcgacaggcc tcgcaggagc gacttgatct ccgaagcgaa
     4021 caccaaccgc ccgccgtcgc gcgaatagaa cagcggcaga atgcccgcgc gatcgcgcgc
     4081 gagcaccagg cggcgcgccc gcacgtccca cagcccgatc gcgaactggc cgttcaaccg
     4141 ctccgcgaag cgcaggccat gctcttcgta gagatggatg atgacctcgg tgtcggtatg
     4201 ggtgtagaag cggtggccgg cctgctcgag ctcggcgcgc agctccacgt agttgaagat
     4261 ctcaccgttg aaggtcaccc aaaccgtgcg atcttcattg tggatcggct gcgcaccgcc
     4321 cgcgaggtcg atgatcgaca ggcgcgcgtg ggcgaggccg acgagctgag gctcatcgag
     4381 gtaagtgccc tgcccgtccg gcccgcggct gtacagcggc tggagcatgc cggcgagctc
     4441 ggtgccggtg accggcgcgc caggtgcgag attgaggatg ccagcgatac cacacatggc
     4501 gcggaatgtt acctgctacg gcggcgcgtc gctgtgcagc acttcgagca atgcaggctg
     4561 cagcgcgtac atcaggtcga tcggctccgc gaggcgcgac gccgcttcgg gctgcgtcac
     4621 catcgtgccc gcgccgaccg gcagcgtcgt gctttcgtgg acgaagccgt tgagccagcg
     4681 ccgcaccaca taacgaccgt cctcggcata agcgtcggca cccaggtagc cggcggcaaa
     4741 catgaacacc cgccgctcgg ccagtgccgc ctcgtcccag atcggcaccc cctggctcag
     4801 ggtgccgtcg cctccggctc cgagcagggc gtgcacggtg ggcgcgatat cgacatgcga
     4861 ggtgcgcgcg gtgacttcgc gcatcgccgt cagcgtgccg ggcgcgaaga tgacgagcgg
     4921 cacctcgaag gaataatccg tgatcgtgcc cgacggcagg ccgggatcct cgacgcgcgt
     4981 gcggacgccg tggtcggcgg tcacgacgat caccgtgtcg tcgagccagc cgccctccgc
     5041 cagccgcgta acgatctccg cgagccacgc atcctgcagg atcatgacat cgcgcccgcg
     5101 ctcgacgacg ctctcgcgcc cgtagagatc gaaccacggg gcgtgaccga tctgcggcac
     5161 gaacagcgtg acgaagcgct cgccggcctg cttgctcgcg acgatgtcgg tgagcatcgc
     5221 gtcgagtgcc atcagatcgt gggtcagtct gtccgcgagc aatgcagcgc gctcgcgccg
     5281 cgccggcgag tcagccggca gtgcctcgat cgtgcgctgc gcgcgctgcg cagcctgcgc
     5341 gaagcgctgt gcctccttcg gatcggccac ataagtcttc gatactccac tgatggcgta
     5401 catggccgtg tcgttctcga acgaatccgg gtagggcagg tacacggcgc ccgagtatcc
     5461 gcgggccgcc aggggcggca cgaggcccgc cccgcttagc gactgcgcct tgcgcaggaa
     5521 tgggccgcgc ccgatcggat acatcccggt tagtatcgag aagatcgcat cgctcgtgta
     5581 tggataggtg gtgaggtgat ggcgcccgac aaacgcgcga tcgagcagca ttgacacgcc
     5641 gggcagcgag tcgcggtcgc cgatcgggtc gtagctgcgc gccggcccgg tctccatgat
     5701 gaacaggatc acgtcgtgac ccgcatcgag cccggcggtc accgccggcg acgagccctc
     5761 cgtctgccgc gcggcgcgcc ggaagagcgc cagcaattcc tcgcgcgatg catgccgagg
     5821 gatatcgtag gcgcctccct gcggcagcat cgcctgcacc atggtgcgca gcgcactgcg
     5881 atgcggtgcg agactgggca cgggcgccgc ggcggcgatc aacgcgagca cgaggctgcc
     5941 ggccagcgct gcgagcgttc cgatctcgat ggtacgcacg aggccccccg caccgacgcc
     6001 gcgcgaacgg cgcgccgccg ccactgcgag cacgacgatg accgtcaccg ccgcgaggag
     6061 cttcagcgca ccccgcgggc tcgcatactc accgatcgcc tccgggaaat cgatggccca
     6121 gaagatcgcg tcctggatca gatcgggcgt caggtagcgc ccgatactgc cgagggactg
     6181 cagctgaaag taatagaacg tgcatagcag aatcgcaacg actgcagcgc tcggtacgcg
     6241 cagcgcacgc ggcaatgcgc gcagcagcag ggcaagaccg accggcaggg cgaagccctg
     6301 gatcatcaga tcgaggcgca ggaagctcag cttctgccac caggcgaact gggactcgtg
     6361 cgaatagccg aggaagcgcg aaatcgccag gtagccggtg gactgcgaga gctgggccag
     6421 cttgacccag acaccgacgg cgcccacgac catgaacatc gctgccagca gcacgatgcc
     6481 ctcggcccgc agcgccgcga gcctcgcg
//
LOCUS       DAOQVY010000132         6458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_13207_length_6458_cov_5.137436, whole genome shotgun sequence.
ACCESSION   DAOQVY010000132 DAOQVY010000000
VERSION     DAOQVY010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13207_length_6458_cov_5.137436"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PKN91_10805"
     CDS             <1..586
                     /locus_tag="PKN91_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNS28597.1"
                     /translation="KASTPAAIEALHAAGLRIVMATGDGVTTAHAVARRLGIDAVHGE
                     VRPQEKIELVRRLQGEGRKVAMAGDGINDAPALAGADVGIAMGTGTDVAMSSAQVTLV
                     RGDLHGIVRARSISLVTVRNMKQNLAFAFLYNALGVPIAAGVLYPLTGLLLSPMIAAL
                     AMSLSSVSVVGNALRLGTLDLDQGQGGTPIGQSV"
     gene            612..1295
                     /locus_tag="PKN91_10810"
     CDS             612..1295
                     /locus_tag="PKN91_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNS28598.1"
                     /translation="MTAPTPGPIACRSCDLYEVCRLCGLIAIGENRGVQRTGALKTLR
                     AGTCLYRAGEPAHSLFAVRQGMLKSVEVGTDGEERIVAVHTPGDVLGTEAFAKRIFVH
                     DVVALQPVVCCELPLSLLSDQYPRVRELSTALINLLSRSSVPRSTLAHGPMRSRVAGF
                     ILDLARRFEERGLDSRSFSLGMTRQEIANLLDTRIETVSRMVQKMSRDREIAIAGQQV
                     TLLSLKRAA"
     gene            1340..2371
                     /locus_tag="PKN91_10815"
     CDS             1340..2371
                     /locus_tag="PKN91_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNS28599.1"
                     /translation="MSLELVCPAGTLPALRAAVDHGADAVYMGFRGATNARNFPGLNF
                     SEAEAATGIAYARERGVRVFVALNVYPSPASWEIGTATIDRAARLGVDALILADPGLM
                     RYASDRYPRLRLHLSVQGSATNWRAIEFYRQQFGIQRAVLPRVLSVTQVARLVGRTAV
                     EIEVFGFGSLCVMVEGRCALSSYATGHSPNSQGVCSPAHAVRWEETPEGRQSRLNGIL
                     IDRYAPGEAAGYPTLCKGRFRVNDTVFHALEEPTSLNALDVLPALAASGVKAIKVEGR
                     QRSPAYVGQVTGLLRRALDLCAASVPPAAPGEAEDGTWTPPADLLAALDALAEGRTHT
                     LGAYHRPWQ"
     gene            2383..3258
                     /locus_tag="PKN91_10820"
     CDS             2383..3258
                     /locus_tag="PKN91_10820"
                     /inference="COORDINATES: protein motif:HMM:NF011991.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNS28600.1"
                     /translation="MKLAVGPILYLWERGAALDFYSALCDEPVDIVYLGEVVCGKRRV
                     LQRGDWPMVARALEDAGKEVVYSTLALIEAESELATMARIVDGSDGRFEANDMAVVQY
                     AAGRPFVAGPHLNIYNDATLQLMASLGARRWVAPVELPLATIAALGASRPASLEIEVF
                     AYGRLPLAFSARCFTARSHDRPKDDCGFICGDYPDGVTLYSREGQPFLTLNGIQTQSA
                     AVQNLVGEVEALRAAGVDILRLSPQSRDFPAVIRAFRAALATRDATATFPAASLPGGY
                     CDGYLRGEAGIDHAG"
     gene            complement(3161..3622)
                     /locus_tag="PKN91_10825"
     CDS             complement(3161..3622)
                     /locus_tag="PKN91_10825"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNS28601.1"
                     /translation="MLALAINQLLRGQLLAGRLDELAGKCFCLSVEDVPLALGFEVVD
                     GGLRRSSRAPHVTIRGSLADFVALARRQEDPDTLFFQRRLAVEGETETGLHLKNLLDG
                     WEYDLPAHLRAVLPGPLASLTLHGLSRLRPAGSRRNTRPAARPPGTWQLHP"
     gene            3790..5271
                     /locus_tag="PKN91_10830"
     CDS             3790..5271
                     /locus_tag="PKN91_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004886738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HNS28602.1"
                     /translation="MTHTSLRDFVHALERSGELKRVPFPVDPRFEMTDLCQRSLKAGG
                     PALWFEQPRGSTMPVLGNLFGTQRRVAAALGYDSVRQFREIGSTLAFLREPHWPDRPR
                     DALGHLPSLLPLLKASPRTVAHPPDYETVQIGPDVDLSQLPVWTCWPEDAGPLITWGL
                     VVTRGTRKPRQNVAPYRQQVIGRNRVIMRWLSHRGGALDFRDWQRDHPAKPFPVAVMI
                     GVDPALLVAATTPIPDSVSEYQFAGLLRRRRSEVRRTAVGLDVCASAEIVLEGHIAPG
                     DEALEGPFGDHTGYYNSQARFPVLTVERLSIRRQAFYHGGYMGRSPWDEPSVLAMSLN
                     DMFVPMLQRSYPEIVDFYLPPEACSYRVAVVSIRKAYPGHARQIMQAVWSSLRQFLYT
                     KFVIVTDDDIDVRNWQEVVWAISTRVDPARDTHLVRRTPIDYLDFASPVAGLGSKLGI
                     DATHKWPAETRRTWGRPLAMSPEIVQRTNAMWAAIFDSHQGGR"
     gene            5315..5845
                     /gene="fabA"
                     /locus_tag="PKN91_10835"
     CDS             5315..5845
                     /gene="fabA"
                     /locus_tag="PKN91_10835"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HNS28603.1"
                     /translation="MSIAEPPRQHSFTREELLACAAGSLFGPGTPRLPTPPMLMVDRI
                     THISAEGGAHDAGEIRAELDIDPSLWFFKCHFVGDPVMPGCLGLDAMWQLIGFHLAWS
                     GHQGLGRALGVDAVRFTGQVLPGMKLVEYQIDIKRVIERRLVMIVGDAAMFADGEKIY
                     TAEGLRVGLFNNPGAA"
     gene            5845..>6458
                     /locus_tag="PKN91_10840"
     CDS             5845..>6458
                     /locus_tag="PKN91_10840"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNS28604.1"
                     /translation="MRRVVVTGFGIVSCLGNDPATVSKSLAEGRSGIRAMPHYPAAGL
                     RSHIAGVPQIVVSEHLDRRDLRFMGDAAAFAAIAMARAIASSGLTEAQVSHPRTGLIA
                     GTGGGSPANIVEAADTLRERGLRRVGATRVPRTMCSTVSACLATSFHIKGVCYSLTSA
                     CATSAHCIGAAAEQIRWGNQDIMFCGGGEEEHWSLTALFDAMGAL"
BASE COUNT         1025 a         2259 c         2157 g         1017 t
ORIGIN      
        1 caaggcctcg accccggcgg cgatcgaagc gctgcatgcc gcgggcctgc gcatcgtcat
       61 ggcgaccggc gatggcgtca ccacggcgca tgcggtcgcg cgccggctcg gcatcgacgc
      121 cgtgcacggc gaagtgcgcc cgcaggagaa gatcgaactg gtgcgcagac tacagggcga
      181 gggccgcaaa gtcgcgatgg cgggcgacgg catcaacgac gccccggcgc tcgccggcgc
      241 cgacgtgggc atcgcgatgg gcaccggcac cgacgtcgcg atgtcgagcg cacaggtcac
      301 gctcgtcaga ggcgacctgc acggcatcgt gcgagcgcgc tcgatatcgc tcgtgacggt
      361 ccgcaacatg aagcagaacc tcgcgttcgc cttcctctac aacgcgctcg gcgtgccgat
      421 cgccgccggc gtgctctacc cgctcaccgg actgctgctc tcgccgatga tcgcggcgct
      481 cgcgatgagc ctgtcttcgg tttcggtcgt gggcaacgcc ctgcggctcg gcacgcttga
      541 cctggatcaa gggcaaggcg ggacgcccat tggacaatca gtttgatcaa tctcaagaca
      601 gtcaactcgc catgacagcg cctacccccg gtcccatcgc ctgccgctcc tgcgacctgt
      661 atgaggtgtg ccgcctctgc ggattgatcg cgatcggcga gaatcgcggc gtacagcgca
      721 cgggcgcgct gaagaccctg cgcgccggca cctgccttta ccgggcaggc gaaccggccc
      781 attccctctt cgccgtgcgc cagggcatgc tgaagtcggt cgaagtcggc accgacggcg
      841 aggagcgcat cgtcgccgtc cacaccccgg gtgacgtgct cggcacggaa gccttcgcga
      901 agcgcatctt cgtgcacgac gtcgtcgcgc tgcagccggt cgtatgctgc gagctgccat
      961 tgtccctgct gagcgaccag tatccgcggg tacgggaact ttccaccgcg ctgatcaacc
     1021 tgctgagccg cagttcggtg ccacgctcga cgctcgccca cggtccgatg cgcagccgcg
     1081 tcgccggctt catcctcgat ctcgcgcgac gcttcgagga gcgcgggctc gacagccgca
     1141 gcttttcgct tggcatgacc cgccaggaaa tagccaacct gctcgacacg cgcatcgaaa
     1201 cggtgagccg catggtccag aaaatgagcc gcgatcggga gatcgcgatc gcgggccagc
     1261 aggtgacctt gctatccctc aaacgggccg cttgatcacc gtcaacacga ggtaagccct
     1321 cagacgcgac catgcgcgga tgagcctcga actcgtctgc ccggccggga cactgccggc
     1381 gctgcgcgcc gctgtcgacc atggcgcgga tgccgtctac atgggttttc gcggcgcgac
     1441 caacgcgcgg aatttcccgg gcctgaattt cagcgaagcg gaagccgcga ccggcatcgc
     1501 ctatgcccgc gagcgcggcg tgcgggtgtt cgtcgcgctc aacgtctacc cctcgcccgc
     1561 gtcctgggag atcggcaccg ctacgatcga tcgcgcggcg cgccttggtg tcgacgcgct
     1621 gattctcgcc gaccccggcc tgatgcgcta cgcgtcggac cgctaccccc gactccgcct
     1681 gcacctcagc gtgcagggtt cggcaaccaa ctggcgcgcc atcgagttct accgtcagca
     1741 gttcggcatc cagcgcgccg tgctgccgcg cgtgctgtcg gtcacgcagg tcgcccgcct
     1801 ggtcggacgc acggccgtgg agatcgaggt cttcggcttc ggcagcctct gcgtgatggt
     1861 ggagggccgt tgtgccctgt cctcctacgc gaccggtcac tcgccgaact cgcagggcgt
     1921 ctgttcgccc gcgcacgcag tgcgctggga ggaaacgccc gaaggtcgcc agtcacgcct
     1981 gaacggcatc ctgatcgatc gctacgcacc gggcgaagcg gcgggctatc caacgctgtg
     2041 caagggccgc tttcgcgtca atgacaccgt attccatgcg ctcgaggagc cgaccagcct
     2101 caacgcgctc gacgtgctgc cggcgctcgc agcaagcggc gtcaaggcga tcaaggtcga
     2161 gggacgccag cgctcgccgg cctatgtagg gcaggtgacg ggcctgctgc gccgcgcgct
     2221 cgatctgtgc gcagcaagcg ttccgccggc cgcgccgggc gaagcggagg acggcacgtg
     2281 gacaccgccc gccgacctgc tggccgcgct cgatgcgctc gccgaagggc gcacgcacac
     2341 cctcggtgcc taccaccggc cgtggcaatg agcgcgcatc ccatgaagct cgccgtcggt
     2401 cctatcctct atctgtggga gcgcggcgca gcgctcgact tctactcggc gctgtgcgat
     2461 gagccggtcg acatcgtcta tctcggcgaa gtcgtgtgcg gcaagcggcg cgtgctgcaa
     2521 cgtggcgact ggccgatggt cgcgcgcgcg ctcgaggacg ccggcaagga agtcgtgtac
     2581 tccaccctcg cgctcatcga ggcggagtcg gaactcgcga cgatggcgcg catcgtcgac
     2641 ggatcagacg ggcgattcga agccaacgac atggcggtcg tgcagtacgc cgccgggcgc
     2701 ccgttcgtcg ccggaccgca tctcaacatc tacaacgacg ccacgctgca gttgatggca
     2761 tcgctcggcg cccgccgctg ggtcgcgccg gtcgaactgc cgctcgcgac gatcgccgcg
     2821 ctcggcgcgt cgcgtcccgc cagtctcgag atcgaagtgt ttgcctatgg gcgcctgccg
     2881 ctcgccttct cggcgcgctg cttcacggcg cgctcccacg atcgcccgaa ggacgactgt
     2941 ggtttcatct gcggcgatta tcccgacggt gtcacgctct actcgcgcga gggtcaacca
     3001 ttcctcacgc tgaacggaat ccagacacaa tccgcagcgg tgcagaacct cgtgggtgaa
     3061 gtcgaggcgc tgcgcgcggc cggcgtcgac attctgcggc tgtcgccgca atcacgggat
     3121 ttcccggccg tgatccgcgc gttccgcgcg gcgctcgcga ctagggatgc aactgccacg
     3181 ttcccggcgg cctcgctgcc gggcgggtat tgcgacggct acctgcgggg cgaagccggg
     3241 atagaccatg cagggtgaga ctcgcgagcg gccccggcag caccgcgcgc agatgcgcgg
     3301 gcaagtcgta ctcccagcca tcgaggaggt tcttcaggtg cagcccggtt tcggtttcac
     3361 cctcgacggc gaggcggcgc tggaagaaaa gcgtgtcggg atcttcctgc cggcgcgcga
     3421 gcgcgacgaa atccgcgagc gagccgcgga tcgtcacgtg cggcgcacgg ctcgaacgcc
     3481 tgagtccgcc atcgacgacc tcgaagccga gcgcgagcgg cacgtcctcg acgctcaggc
     3541 agaagcactt gcccgcgagt tcatcgagcc ggccggcgag aagctggccg cgcagcagct
     3601 gattgatcgc gagcgcgagc atctgtgcct gcaacggcgt cggcgcaaca cggagcacac
     3661 ggtggagaac ggccatggga tcgggcagca tgatgggctc gcgcagggac gaggccggta
     3721 ttcatgccat gaacgcggcc gcggccactt gatcccggtc aacacggccg cccgcagcga
     3781 gaccgccgga tgacccacac gagcctgcga gacttcgtgc acgcgctcga gcgcagcggc
     3841 gagctgaagc gcgtgccctt ccccgtcgac ccgcgattcg agatgaccga cctttgccag
     3901 cgcagcctca aggccggcgg gccggcgctg tggttcgagc agccgcgcgg ctcgaccatg
     3961 ccggtgctcg gcaatctctt cggcacccag cgacgcgtgg ccgcggctct tggctacgac
     4021 agcgtgcggc agtttcgcga gatcggctcg acgctcgcct tcctgcgcga accgcactgg
     4081 cccgaccgcc cgcgcgacgc gctcggccac ctgccctcgc tgctgccgct gctcaaggcc
     4141 tcgccgcgca cggtggcgca tccacccgac tacgagacgg tgcagatcgg acccgatgtc
     4201 gacctgtcgc agctgcccgt ctggacgtgc tggccggagg acgccggtcc gctgatcacc
     4261 tggggcctcg tcgtaacgcg cggcacgcgc aagccgcgcc agaacgtggc gccctaccgc
     4321 cagcaggtca tcggccgcaa tcgcgtaatc atgcgctggc tgtcgcatcg cggtggcgcg
     4381 ctcgacttcc gcgattggca gcgcgaccac cccgccaagc cgtttcccgt ggccgtcatg
     4441 atcggcgtgg accccgcact gctggtcgcg gcgaccacac ccattccgga cagcgtgtcc
     4501 gagtaccagt tcgccggcct gctgcgtcgc cggcgcagcg aggtgcgtcg caccgccgtc
     4561 ggcctcgacg tgtgcgcaag cgcggagatc gtgctcgagg ggcacatcgc accgggtgac
     4621 gaggcactcg aggggccgtt cggcgaccac accggctact acaacagcca ggcgcgcttc
     4681 ccggtgctca ccgtcgaacg gctctcgata cgacggcagg ctttctatca cggaggttac
     4741 atgggccgct cgccgtggga cgagccctcc gtgctcgcga tgtcgctcaa tgacatgttc
     4801 gtgccgatgc tgcagcggag ctacccggaa atcgtcgatt tctacctgcc gcccgaggcc
     4861 tgctcctacc gggtcgcggt cgtgagcatc cgcaaggcgt accccggcca cgcgcggcag
     4921 atcatgcagg cggtgtggtc gagcctgcgg cagttcctct acaccaagtt cgtgatcgtg
     4981 accgacgatg acatcgacgt gcgcaactgg caggaggtgg tctgggcgat atcgacacgg
     5041 gtcgatcccg cgcgcgacac ccatctcgtg cggcgcactc cgatcgacta cctggatttc
     5101 gcgagcccgg tcgccggcct tggatcgaag ctcggcatcg acgcgaccca caagtggccc
     5161 gccgagaccc gccgtacctg gggtcgtccg ctcgcgatga gccccgagat tgtgcagcgc
     5221 accaacgcga tgtgggccgc catctttgat tcgcatcaag gcgggcgcta gggtaccggg
     5281 ctgtaatcag tctatgcgat cgagaagtac agtggtgagc atcgccgaac cgccccgaca
     5341 acacagtttc accagggagg aactgctggc ctgcgccgca ggcagcctgt tcggccccgg
     5401 cacgccgcgc ctgccgaccc cgccgatgct gatggtcgac cggatcaccc acatctcggc
     5461 ggaggggggt gcccacgacg caggcgagat ccgcgccgaa ctcgacatcg acccctcgct
     5521 gtggttcttc aagtgtcatt tcgtcggcga cccggtgatg ccgggctgcc tcgggctcga
     5581 cgcgatgtgg caattgatcg gttttcatct cgcgtggagc ggtcaccagg gcctcgggcg
     5641 ggcgctcggc gtggacgcag tgcgcttcac ggggcaggtg ctcccgggca tgaagctcgt
     5701 cgaataccag atcgacatca agcgcgtgat cgagcgccgg ctcgtgatga tcgtgggcga
     5761 cgccgcgatg ttcgcggacg gcgagaagat ctacaccgcg gaggggctgc gcgtaggcct
     5821 cttcaacaac ccaggagccg cctgatgcgc cgcgtggtcg tcacgggatt cggcatcgtg
     5881 tcctgcctcg gcaatgaccc cgccacggtc agcaagtcgc tggcggaggg ccgctccggc
     5941 atccgcgcga tgccgcacta tccggccgcc ggtctgcgca gccatatcgc gggcgtcccg
     6001 cagatcgtgg tcagcgagca cctcgaccgg cgcgacctga gattcatggg cgacgcggcc
     6061 gcattcgccg cgatcgcgat ggcgcgggcg atcgccagca gcgggctcac cgaggcgcag
     6121 gtgagtcatc cgcgcaccgg gctgattgcc ggcaccggcg gcggctcacc cgccaatatc
     6181 gtcgaagccg ccgacacgct gcgcgagcgc ggcctgcgca gggtgggcgc aacgcgcgtg
     6241 ccacgcacga tgtgcagcac ggtcagcgca tgcctggcga cgagcttcca tattaaaggc
     6301 gtctgctact cgctgacctc ggcctgcgcg acgagcgcgc attgcatcgg agccgccgcc
     6361 gaacagatcc gctggggcaa ccaggacatc atgttctgcg gcggcggcga ggaggagcac
     6421 tggtcgctca cggcactgtt cgacgcgatg ggcgcgct
//
LOCUS       DAOQVY010000133         6431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_13275_length_6431_cov_5.227415, whole genome shotgun sequence.
ACCESSION   DAOQVY010000133 DAOQVY010000000
VERSION     DAOQVY010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13275_length_6431_cov_5.227415"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1602
                     /locus_tag="PKN91_10845"
     CDS             130..1602
                     /locus_tag="PKN91_10845"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNS28605.1"
                     /translation="MVHATVKVSPVAGGRLVRLDDTRARAAPGVIDVVRIDGGVAVVA
                     ASWWQAHKALELLELAFEGGADGESTASVDATLGNALAQGGLVAREEGDVEAALRSSA
                     RVLEFEYSVPYLAHAAMEPMSCVAHVAEGRCTVWAPTQGPTVAHAAAARAAGVPPENV
                     TVRRMFLGGGFGRRYETDFVTQAVQISRAIGRPVKLLWSREEDLTHDFYRPAARMRYR
                     AALAADGTPAAVDVVGACGSILARMRGGLGSDRVDAASVDGVLDRELPLAHVRIRHTV
                     VDTPLQVGMWRSVSHSQNAFFKESFMDELAHAARRDPYRYRRKLLAGTRQAAVLELAA
                     AKAGWEKPPPEGHARGIAIVACYGSIVAQVVELSRDGDAPRVHRVVCAVDCGIAIDPY
                     NIAAQMESGVVYGLSAALWGEITVSGGAVEQRNFHQYRVLRMNEMPRVETYIVPSSDA
                     PGGIGECAVPPIAPAVANAWFALTGQRVRRLPFVSAVNAQ"
     gene            complement(1596..2999)
                     /locus_tag="PKN91_10850"
     CDS             complement(1596..2999)
                     /locus_tag="PKN91_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNS28606.1"
                     /translation="MRISHRLAVITSCLLAAAQVQAGEAEFRSLYKELVEINTTLSAG
                     SCTDAAAAMRTRLSNAGYPDTDLHLITAPDRPKDGNLIAVLHGTDAGLKPVMLLAHID
                     VVEANREDWERDPFTLTEEGGYFYARGAADDKAMAAIFTDAMVRFRLEGYRPERGIKL
                     ALTCGEETPNTFNGVKYLLQYHRGLMDAAFALNEGGGGRLDGASDRYLYNGVQAGEKL
                     YQDFTLETVNEGGHSSRPVADNAIYQMMRALAKVQALDFPTEFNDATRSYFARFGEIV
                     GGTRGADMKAVAATGDAPAVERLKQDPSLNSMLHTTCVATMIEGGHAPNALPQRVTAN
                     VNCRIFPGRAPEEIRQALIDAIGDAGVKVTFQAEPEKAGPPPKLTPEIMGPIEKLTAE
                     MFPGVPVIPAQSSGGTDGRFLTPAGIPTYGVSGIFSDGATTNAHGVNERIRVQSLMEG
                     REFLYRLIKLYASPSAH"
     gene            complement(3235..3720)
                     /locus_tag="PKN91_10855"
     CDS             complement(3235..3720)
                     /locus_tag="PKN91_10855"
                     /inference="COORDINATES: protein motif:HMM:NF022606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2938 domain-containing protein"
                     /protein_id="HNS28607.1"
                     /translation="MKYLLDILWTGLGATVATDLWTILRRRLLGTPLPDYGLVGRWIA
                     YMPRGRFVHDPIAATPAVRGERTLGWIAHAIIGVTFAALVPAVFGHAWLDNPSVVPAL
                     LVGIGTVAAPFLVMQPAMGAGIAAHRIPNPRAARLQSLVTHTIFGLGLYFAALVSSVI
                     R"
     gene            3794..4204
                     /locus_tag="PKN91_10860"
     CDS             3794..4204
                     /locus_tag="PKN91_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS28608.1"
                     /translation="MRDLDINEVARLSGLPASTLRYYEEKGLVQSVGRRGLRRLFDPG
                     ILERLALISLGRAAGFSLDQMARMFAPGQRPRIDRLLLAKKADELGAAIRKLTAMRDG
                     LRHAAVCPASSHMECPTFRRLLGIASSPARRRIR"
     gene            complement(4265..4756)
                     /locus_tag="PKN91_10865"
     CDS             complement(4265..4756)
                     /locus_tag="PKN91_10865"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HNS28609.1"
                     /translation="MTKPAPPDTAAITSFWQSARRLYPDAVHGDSFRIRALGDTPEMM
                     NSILGHVLRGEKKGTFALPWLHAKDPSATPRIGDYVVLVDYEGRPAALVTTTQLENVT
                     FDTISGNHTALDGPRMRDPAAWKTLHQAFWTKQLAAAGLQFSKDMPVVVEHFELLHPL
                     REP"
     gene            complement(4989..5867)
                     /locus_tag="PKN91_10870"
     CDS             complement(4989..5867)
                     /locus_tag="PKN91_10870"
                     /inference="COORDINATES: protein motif:HMM:NF025091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HNS28610.1"
                     /translation="MNSTDTPAARLRAAIAAPRILVAPGCYDALSANLIERAGFSAAY
                     LSGASIAYTRFGRPDIGLVGMSEVAETVSQIRERVELPLIVDGDTGFGNALNVQRTIR
                     LFERSGASAIQLEDQQMPKRCGHLSGKTLVSVKEMAGKIRAACDARADSATVIIGRTD
                     ALAVEGIDAALDRAERYLEAGADVLFIEALPDVAAMQTALTRFRDRAPLLANMVEGGK
                     SPLLPAAELQRLGFALAIFPGAMVRVQTFAAQRYLETLKRDGTTSTILGEMLDFEALN
                     QLLGTEAWLERGKLYE"
     gene            complement(5879..>6431)
                     /locus_tag="PKN91_10875"
     CDS             complement(5879..>6431)
                     /locus_tag="PKN91_10875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28611.1"
                     /translation="RPARLPADPGGGMSAPTTVFTGRAWVFGDRIDTDLLAPGHAIRK
                     PIEELAKHCLEAVRPEFARDIVRGDVVVGGEGFGIGSSREQAAQALRYLGVSVVVAKS
                     AARIFYRNAFNLGLPVLILPEAGQIADGARLEVDLARGLVRDMTGGREFRGEAVPDFL
                     LEIVADGGLIPNLHKRLHGKPAA"
BASE COUNT         1025 a         2030 c         2270 g         1106 t
ORIGIN      
        1 cctcccgccg agccgcagct caaacctgcg tccgaattcc ggttgatcgg ggcacggacc
       61 gcgcgcaaag actgtgcggc aaaaatcgat ggtagcgcgc agttcgcgag cgatatcgtg
      121 ctgccgggga tggtgcacgc taccgtgaaa gtgtcgccgg tcgcgggagg gaggctcgtc
      181 cggctcgacg atacgcgtgc gcgagccgcg cccggggtga tcgatgtcgt gcgcatcgac
      241 ggcggcgtcg ccgtcgtcgc ggcgagttgg tggcaggcgc acaaggcgct cgagctgctc
      301 gagcttgcgt tcgaaggtgg cgctgacggc gaaagcacgg cctcagtgga tgccacgctg
      361 ggcaatgcgc tcgcgcaggg aggtctcgtc gcacgcgagg agggcgacgt cgaggcggcg
      421 ctgcgctcga gtgcgcgcgt gctcgagttc gagtactccg tgccctatct cgcgcatgca
      481 gcgatggagc cgatgagctg cgtcgcgcat gtcgcggagg gccgctgtac ggtatgggcg
      541 cccacgcagg gcccgaccgt cgcgcacgcg gccgcggcgc gggccgccgg tgtgccgccc
      601 gagaacgtca ccgtccgccg catgtttctc ggcggcggct tcggccgccg ttacgagacg
      661 gacttcgtca cgcaggccgt gcagatctcg cgcgccatcg gccggccggt gaaactgctg
      721 tggagccgcg aagaagacct gacgcacgat ttctaccggc ctgcggcgcg catgcgctac
      781 cgtgcggcgc ttgcggcgga cggcacacca gccgcggtcg acgtggttgg tgcctgcggg
      841 tcgatcctcg cgcgcatgcg cggcggtctc ggtagcgaca gggtcgacgc ggcgagcgtc
      901 gacggcgtgc tcgatcgtga gctgccgctg gcccacgtgc gcattcgcca cacggtggtg
      961 gacacgcccc tgcaggtagg catgtggcgc agcgtcagcc attcgcagaa cgcattcttc
     1021 aaggaatcgt tcatggacga actcgcgcat gcggcgcggc gtgatccgta caggtaccgg
     1081 cggaagctgc tggcgggtac gcggcaggcc gcggtgctcg agctcgccgc cgcgaaagcg
     1141 ggctgggaga agccgccgcc cgaaggtcac gcgcgcggca tcgcgatcgt ggcctgctac
     1201 ggcagcatcg tcgcgcaggt cgtcgaactg tcgcgtgacg gcgacgctcc tcgcgtccat
     1261 cgcgtcgtct gcgcggtcga ctgcggcatc gcgatcgatc cgtacaacat cgccgcgcag
     1321 atggagagcg gcgtggtcta cggcctctcg gcggcgctgt ggggcgagat caccgtgagc
     1381 ggcggggccg tcgagcagcg gaacttccac cagtatcgcg tgctgcgcat gaacgagatg
     1441 cctcgtgtcg agacctacat cgtgccgagc agcgacgcgc ccggcggcat cggcgagtgc
     1501 gcagtgccgc ccatcgcgcc ggccgtggcg aacgcctggt tcgcgctgac cggacagcgt
     1561 gttcgccgcc tgccgttcgt ttcagccgtg aatgctcaat gagccgacgg actcgcatac
     1621 agcttgatca gccggtagag gaactcgcgc ccctccatca gcgactggac gcggatgcgt
     1681 tcgttcacgc catgggcgtt ggtcgtggcg ccgtcggaga agatcccgga gacgccgtag
     1741 gtcggaatgc cggcgggagt gaggaagcgg ccatcggtgc cgccggatga ctgtgccgga
     1801 atgaccggca cgccgggaaa catctcggcg gtcagcttct cgatcggtcc catgatctcg
     1861 ggagtcagct tcggcggtgg gccggctttc tcgggctcgg cctggaacgt caccttcacg
     1921 cccgcatcgc cgattgcgtc gatcagcgcc tggcggatct cctcgggcgc gcggcccggg
     1981 aagatgcggc agttgacgtt tgccgtgacg cgctggggca gggcgttcgg tgcgtgcccg
     2041 ccctcgatca tggtcgcgac gcaggtggtg tgcagcatgc tgttcaggct cgggtcctgc
     2101 ttgagtcgct cgacggccgg cgcatcgcca gtcgctgcga ccgccttcat gtcggcgcct
     2161 cttgtgccac cgacgatctc gccgaacctg gcgaagtagc tgcgcgttgc atcgttgaac
     2221 tcggtgggga agtccaatgc ctgcaccttc gccagcgccc gcatcatctg gtagatggcg
     2281 ttgtccgcca ccggtcgcga agagtggccg ccctcattga cggtctcgag cgtgaagtcc
     2341 tggtacagct tctcgccggc ctgcacgccg ttgtacagat accggtcgct tgcgccgtcc
     2401 agccgtccac cgccgccttc gttgagtgcg aacgccgcgt ccatcagtcc gcgatgatac
     2461 tggaggaggt atttgacgcc gttgaatgtg ttgggcgttt cctcgccgca ggtgagcgcg
     2521 agcttgatgc cgcgctccgg ccggtagccc tccagcctga agcggaccat cgcgtcggtg
     2581 aatatcgcgg ccatggcctt gtcgtcggca gcgccccgcg cgtagaagta gccgccttct
     2641 tcagtcagcg tgaacggatc gcgttcccag tcctcgcgat tggcttccac tacatcgata
     2701 tgggccagca gcatcaccgg tttcaggccg gcatcggtac cgtgcagcac ggcgatgagg
     2761 ttgccgtctt tcggccggtc gggtgcggtg atgaggtgca ggtcggtgtc ggggtatccc
     2821 gcattcgaca ggcgcgttct cattgccgcc gccgcgtcgg tgcacgagcc cgccgagagc
     2881 gtggtgttga tctcgaccag ttccttgtaa agcgagcgga actcggcttc gccggcctgc
     2941 acctgggcgg cagcgagcag acaggaagtg atgacggcaa gacggtggct gattcgcatg
     3001 attcaccgtg cggatgggca aagagggggc atcatacggc ggctcacggg gctgccgcca
     3061 ttgcgatgcg ccagtccttg ccatggcggc gctgcagctg gggcgtcagg ccgtcagttg
     3121 tgagacgacc ctgcgcaccc acggagcgat gccggtcatg atggccgaca gcagggcgcc
     3181 gagcagaaca ggtgcagatc gaacgggaag ttgcatgggt tcctcctgga ggattcagcg
     3241 aatgacgctc gacaccaggg ctgcgaaata cagcccgagg ccgaagatgg tgtgggtcac
     3301 gaggctctgc agccgcgcgg cgcgggggtt cggtatgcga tgtgcggcga tgccggcgcc
     3361 catcgccggt tgcattacga gaaaaggtgc cgccacggtg ccgatgccga cgagcagcgc
     3421 cggaacgact gacggattgt ccaaccacgc atggccgaag actgcgggca cgagcgccgc
     3481 aaacgtcacg ccgatgatcg cgtgtgcgat ccatccgagc gtgcgctcgc cgcgcaccgc
     3541 gggtgtcgcg gcaatggggt cgtgtacgaa gcgaccccgc ggcatgtacg caatccagcg
     3601 acccacgagg ccgtagtcgg gcagcggcgt tccgaggaga cgcctgcgaa ggatcgtcca
     3661 taggtccgtc gccaccgtgg cccccaggcc cgtccagagg atatcgagca gatacttcat
     3721 gttccggtcc ccgcttgcct ttcatcggta ccggcgccag agtgccactt caagtgcact
     3781 tgaggtcaag gggatgcgcg atctggacat caacgaagtg gccaggcttt cgggcttgcc
     3841 ggcgtcgacc ctgcgctatt acgaagagaa ggggttggtg cagtcggtgg gccggcgggg
     3901 actcaggcga ctgttcgatc ccgggatcct cgagcgcctg gcgctgattt cgctcggccg
     3961 cgccgcgggc ttcagcctcg atcagatggc acgaatgttc gctccgggcc agcgcccccg
     4021 catcgatcgg ctcctgctcg cgaagaaggc ggatgagttg ggtgccgcga tccgcaagtt
     4081 gacagcgatg cgtgacgggc tgcggcatgc cgccgtctgt ccggcgtcga gccacatgga
     4141 atgcccgacg ttccggcggc tactcggaat cgcgagctcc ccggcgcgcc ggcgcattcg
     4201 atgaattgtc ccgctgatgt gaccagcgtg tcgcgttgcg gccgccattg cgcggccggg
     4261 agagttacgg ctcgcgtaac gggtgaagca gctcgaagtg ctccacgaca acaggcatgt
     4321 ctttggagaa ttgcaggccg gccgccgcga gctgtttcgt ccagaatgcc tgatgcagcg
     4381 ttttccaggc ggccggatcg cgcatgcgcg ggccatccag cgcggtgtga ttcccgctga
     4441 tggtgtcgaa cgtgacgttc tcgagttgcg tcgtggtgac caacgctgcc ggcctgccct
     4501 cgtagtccac gagtaccaca taatcgccga tcctgggcgt ggccgaaggg tctttcgcat
     4561 gaagccaagg tagcgcgaac gtgccctttt tctcgccacg caggacatgg ccgaggattg
     4621 agttcatcat ctccggcgta tcgcccagtg cccgtatgcg aaagctgtca ccgtgcaccg
     4681 catcggggta aaggcgtctc gccgactgcc agaacgaggt gatggcagca gtgtcgggcg
     4741 gtgcgggttt ggtcatgata tatgtcggga tcgagacgct ggccgggcgt gtagatctcc
     4801 acccacgctg ctgatctcat ggccgagcag cgtcggtggg gccgggcggg ctatcgtacc
     4861 aatgtacacc gcttgtccag gcaatacgag agcgtacacg ccagggcgga atcgtcgatg
     4921 cacaaagtcg atctctgctg tagcgccggg gccctcagcc cggcggcaac ctggagccgg
     4981 tcgcaacgct actcgtagag cttgccccgc tcgagccacg cctcggttcc gagcagctgg
     5041 ttcagcgcct cgaagtcgag catctcgccg aggatcgtgc tcgtcgtgcc gtcgcgcttg
     5101 agcgtttcga ggtagcgctg tgcggcgaaa gtctgcacgc gcaccatcgc gccggggaag
     5161 atcgcgagcg caaagccgag ccgctgcagt tcggcggccg gcaacagcgg cgacttgccg
     5221 ccctcgacca tgttggcaag gagtggggcg cggtcgcgaa agcgggtgag ggcggtctgc
     5281 atggccgcga catcgggcag cgcctcgatg aacaacacgt cggcgcccgc ctcaagatag
     5341 cgctcggcac gatcgagcgc ggcgtcgatt ccctcgaccg cgagcgcgtc ggtgcgacca
     5401 atgatgaccg tagccgagtc ggcacgcgcg tcgcaggcgg cgcggatctt gcccgccatt
     5461 tccttcacgc tgacgagcgt cttgccggac aggtggccgc agcgtttcgg catctgctgg
     5521 tcctcgagct ggattgccga ggcgcccgag cgctcgaaga gccgtatggt gcgctgcacg
     5581 ttgagtgcat tgccgaaacc agtgtcgccg tcgacgatca gcggcagctc gacccgctcg
     5641 cggatctgcg agacagtctc ggccacctcg ctcatgccga cgaggccgat gtcgggccgg
     5701 ccgaagcgcg tgtaggcgat gcttgcgccg gacagatagg cagcggagaa acccgcgcgc
     5761 tcgatcagat ttgcggacag tgcgtcgtag cagcctgggg cgacgaggat ccgtggcgcg
     5821 gcgatggccg cacgcaggcg ggctgcgggg gtgtcggtcg agttcatgtg aggttgtgct
     5881 atgcggccgg tttgccgtgc agtcgtttgt ggaggttcgg tatcaatccg ccgtcggcga
     5941 cgatttcgag caggaagtcc ggtacagctt cgccgcggaa ctcgcggccg ccggtcatgt
     6001 cgcgaacgag gccgcgcgcg agatccactt cgagccgcgc gccgtcagcg atctgcccgg
     6061 cctcaggcaa gatcagaacc ggcagcccaa ggttgaacgc gttgcgataa aagatgcggg
     6121 ccgcggattt cgcgactacg accgaaaccc cgaggtagcg caaggcctgc gcggcctgct
     6181 cgcgcgagga gccgatgccg aagccttcgc cgccgaccac gacatcgccg cggacgatgt
     6241 cgcgcgcgaa ttccggtcgc acggcttcga ggcaatgctt cgcgagctcc tcgatgggtt
     6301 tgcggatcgc gtgacccggc gcgagcaggt cggtgtcgat gcgatcgccg aacacccagg
     6361 cacggccggt gaacactgtc gtgggggcgc tcatgccgcc gcccgggtcg gcaggaagtc
     6421 gcgcgggtcg c
//
LOCUS       DAOQVY010000134         6202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_13972_length_6202_cov_6.066862, whole genome shotgun sequence.
ACCESSION   DAOQVY010000134 DAOQVY010000000
VERSION     DAOQVY010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13972_length_6202_cov_6.066862"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PKN91_10880"
     CDS             complement(<1..543)
                     /locus_tag="PKN91_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS28612.1"
                     /translation="MREAQRPIAASAQTGDAVVEVSGLRYSVGRREIFAGLDLVARRG
                     RITAIMGPSGTGKTTLLRLITGQVAPSAGRVQVWGENVATLGQRGLFALRRRIGMLFQ
                     NGALLTDLDVFENIAFPVREHTNLTEPLIRQLVLTKLHAVGLRGAARMMPAELSGGMG
                     RRVALARAVVMDPELLIYDEP"
     gene            complement(566..1588)
                     /locus_tag="PKN91_10885"
     CDS             complement(566..1588)
                     /locus_tag="PKN91_10885"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class I"
                     /protein_id="HNS28613.1"
                     /translation="MDKADLERVAQAMVARDKGILAADESSGTIKKRFDAIKLESTEE
                     HRRAYREMLFTTPGIGQWISGVILYDETLRQKTREGTPFAQYLAAAGVLPGIKVDKGA
                     KALAGFPGDTITEGLDGLRERLVEYYQLGARFAKWRAVIDIADGVPSRFAIEANAHAL
                     ARYAALCQEQQIVPIVEPEVLMDGGHSIERCEEVTSEVLAAVFSQLHRHGVHLEGMVL
                     KPNMVISGKKAANRASPEQVAEATVRTLKRHVPTAVPGIAFLSGGQSSPEATLHLSLM
                     NKLGPLPWQLTFSYGRALQDTAIRAWGGNPANFAAGQKELAKRARLNSLATTGRYAAA
                     QEAEAA"
     gene            1685..2161
                     /locus_tag="PKN91_10890"
     CDS             1685..2161
                     /locus_tag="PKN91_10890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HNS28614.1"
                     /translation="MLRISRMTDYATVVLAALARSPAPQMTAAALARRTRIAAPTVSK
                     LLKQLHRAGLVASTRGLHGGYQLARGAEQISAAAILDALEGPFALTECSTAHGQCDIE
                     GHCRVSRAWQSVNGAIRRSLQDVSLAQLAGLDEQPANLPALEHAVKFPGLRTRGAP"
     gene            2158..3603
                     /gene="sufB"
                     /locus_tag="PKN91_10895"
     CDS             2158..3603
                     /gene="sufB"
                     /locus_tag="PKN91_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNS28615.1"
                     /translation="MTDATELEALVGRSYRHGFVTDIDSDTVPPGLDEQVVRLISRKK
                     GEPDFLLEWRLKAFRHWLRMREPRWAHLRFNPIDYQDISYYSAPRVDANAPKSLDEVD
                     PKLLATYEKLGVPLHERARLAGVAVDAVFDSVSVATTFKERLSKAGVIFCSFSDAVRD
                     HPELVEKYLGTVVPYTDNFFATLNSAVFSDGSFVYVPKGVRCPMELSTYFRINAAKTG
                     QFERTLIIADEGSHVSYLEGCTAPMRDENQLHAAVVELIALDDAKIKYSTVQNWYPGD
                     ENGVGGIYNFVTKRGECRGRNSHISWTQVETGSAITWKYPSCVLRGAGSVGEFYSVAT
                     ATNYQQADTGTKMIHIGADTRSTIVSKGISAGHGQNTYRGLVRIMPGAHGARNFTQCD
                     SLLMGGECGAHTFPYVEVKNATATVEHEASTSKISEDQLFYCLQRGISQEDAVNMIVS
                     GFCKSVFKELPMEFAVEAQNLLAVTLEGSVG"
     gene            3606..4358
                     /gene="sufC"
                     /locus_tag="PKN91_10900"
     CDS             3606..4358
                     /gene="sufC"
                     /locus_tag="PKN91_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNS28616.1"
                     /translation="MLKINDLHVTVGHRKVLDGLTLEVPTGEVHAIMGPNGSGKSTLA
                     YALAGRPGYEAESGSVQFDGQDLLAMPAEERARAGVFLGFQYPVEIPGVNNVYLLKAA
                     LNARRKAAGLPEVDAFEFLELVRGKMKFMQMDEGFLSRGVNEGFSGGEKKRNEILQML
                     VLEPRLALLDETDSGLDIDAMKVVARGIESLRGPERSIVLVTHYQRLLDHVRPDRVHV
                     LSRGRIVRSGDAALALELERRGYDWVLGADAA"
     gene            4355..5650
                     /locus_tag="PKN91_10905"
     CDS             4355..5650
                     /locus_tag="PKN91_10905"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNS28617.1"
                     /translation="MSAITQLASDHEAVLAALPAGEVSHAARRATLEMLAEQGLPGPR
                     EENWRYANLRPLEQLRFTPRLTPDAAALAQATELLPAPLAGFDRYVVVDGLFAAPLSA
                     PTGTAGDVEVALQAGIDAGYGSAGGDARFGALASAFAPQAIRVAAGAGAARDIEVVFV
                     ATAAGSEAASYPALLVKLAAQAHLRLVERHIGACDAATFANAALAIEVGAAGQLRHVR
                     VQQAGAQATHFETLRAEVADSAEYLLDTVALGAQSARSTTHARLAGRGARIAYSRSVA
                     VDATRVNDAVVVIEHAAPATITAQDYRGIAAGRARVAFNGHVIMRANAAGSASSQSLR
                     SLLAGPGTEADVRPQLEIYTDDVKASHGATAGKIDEQMLFYLLSRGIERRTAQSLLKW
                     AFLTELIARIPIPDLRAQVERAFAQRFTGEDAAVTRELV"
     gene            5650..>6202
                     /locus_tag="PKN91_10910"
     CDS             5650..>6202
                     /locus_tag="PKN91_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNS28618.1"
                     /translation="MVTAAAIATRRLDAARVRADFPILARKVNGQPLVYLDSAASAQR
                     PRAVIAAVDHYETHLHANVHRGVHTLSQAATDAFEGARERVRRFMNARSTKEIVFTRG
                     TTEAINLVAQSFARPRLGPGDEILITWLEHHANIVPWQMVCAATGATLRVAPIDERGE
                     LRFDEFQALLSPRTKLVATAHVSN"
BASE COUNT          994 a         2166 c         2069 g          973 t
ORIGIN      
        1 gggctcatcg tagatcagca gctccgggtc catgaccacg gcgcgcgcca gcgcgacgcg
       61 ccgacccatg ccgcccgaca gctcggcggg catcatgcgc gccgcaccgc gcagccccac
      121 cgcgtgcaac ttggtcagca cgagctggcg gatcagtggt tcggtgagat tggtgtgctc
      181 gcgcaccggg aaggcgatgt tctcgaacac gtcgaggtcg gtgagcagcg cgccgttctg
      241 gaacagcatg ccgatgcgcc ggcgcagcgc gaacagcccg cgctggccga gcgttgcgac
      301 attctcgccc cacacctgca cgcgcccggc acttggcgcg acctgcccgg tgatcaggcg
      361 cagcagcgtg gtcttgccgg tcccgctcgg gcccatgatg gccgtgatcc ggcctcgccg
      421 ggcgacgagg tcgaggccgg cgaaaatctc gcggcggccg acggaatagc gcagccccga
      481 cacctcgacc acggcgtcgc cggtctgcgc cgacgcggcg atcggccgtt gtgcttctct
      541 cacgcggtcg ggtcaccgtc gccgctcaag cggcttcggc ctcctgcgcg gcagcgtaac
      601 gaccggtggt ggcgaggcta ttgaggcgcg cgcgcttggc gagttccttc tggcccgccg
      661 caaaattggc cgggttgcca ccccaggccc tgatcgcggt gtcctgcagc gcgcggccgt
      721 agctgaaggt gagctgccat ggcagcggcc cgagcttgtt catcagcgac aggtgcaggg
      781 tcgcctcggg cgatgactgc ccaccggaga ggaacgcaat gcccggcacc gcggtgggta
      841 cgtggcgctt gagcgtgcgc acggtcgctt cggcaacctg ctcggggctc gcccggttcg
      901 cggccttctt gccggaaatc accatgttgg gcttcaggac catgccttcg aggtggacac
      961 cgtggcggtg caactggctg aacacggcag cgagcacctc gctcgtgacc tcctcgcagc
     1021 gttcgatcga atgcccgccg tccatcagga cttcgggttc gactatcggc acgatctgtt
     1081 gctcctggca cagcgctgcg tagcgggcga gcgcgtgggc attcgcctcg atggcgaacc
     1141 gggacggcac gccgtcggca atgtcgatga ccgcgcgcca tttcgcgaag cgcgcgccga
     1201 gctggtagta ctcgacgaga cgctcgcgca ggccgtcgag gccctcggtg atggtgtcgc
     1261 cgggaaagcc ggcgagcgcc ttcgcgccct tgtcgacctt gatgccgggc agcacgcccg
     1321 ccgcagcgag gtactgcgcg aacggcgtac cctcgcgcgt cttctgacgc agggtttcgt
     1381 cgtacaggat cacgccgctg atccactggc cgatgccggg cgtcgtgaac agcatctcgc
     1441 gataggcgcg gcgatgctcc tccgtggact cgagcttgat cgcgtcgaag cgcttcttga
     1501 tggtgcccga gctttcgtcg gccgcgagaa tgcccttgtc cctggccacc atggcctgcg
     1561 cgacgcgctc gagatccgct ttgtccatcg tcctgctctc cgattccaga tgccagaagt
     1621 agccgtcaat tatagccgaa ggaccttttg cagcgccgcc taaagaggac taaaatagtc
     1681 ccttatgttg cgcatcagcc ggatgaccga ttacgcgacc gtcgtgctcg cggcgctcgc
     1741 acgctcgcct gccccgcaga tgacggccgc ggcgctcgcc aggcgcaccc gcatcgcggc
     1801 acccaccgtc agcaagctgc tgaagcagct gcatcgcgcc ggactcgtgg cctcgacccg
     1861 tggcctgcat ggcggctacc agctggccag gggcgccgag caaatctcgg ccgccgcgat
     1921 cctcgatgcc ctcgaagggc cgttcgcgct gacggagtgc tccaccgccc acggccagtg
     1981 cgatatcgag ggccattgcc gcgtctcgcg cgcctggcag agcgtcaacg gtgcgatccg
     2041 ccgttcgctt caggatgtca gcctggcgca gctcgccggc ctcgacgaac agcccgcgaa
     2101 cctgcccgcg ctggagcatg cggtgaagtt ccccggcctg cgcacacgag gcgcaccatg
     2161 accgacgcca ccgaactcga ggccctggtc gggcgcagct accgccacgg cttcgttacc
     2221 gacatcgact cggacacggt gccgcccggc ctcgacgagc aggtcgtgcg cctgatctcg
     2281 cgcaagaagg gtgagccgga cttcctgctc gaatggcgcc tcaaggcgtt tcgtcactgg
     2341 ctccggatgc gcgagccgcg ctgggcgcat ctgcgcttca atccgatcga ctaccaggac
     2401 atctcctatt actcggcgcc gcgcgtcgat gcgaacgcac ctaagagcct cgacgaggtc
     2461 gatccgaagc tgctcgcgac ctacgagaag ctcggcgtgc cgctgcacga gcgtgcccgc
     2521 ctcgccggcg tggcggtcga cgcggtgttc gacagcgtct cggtcgcgac gaccttcaag
     2581 gagaggctgt ccaaggccgg cgtgatcttc tgctcgtttt cggatgcggt gcgcgaccac
     2641 ccggaactcg tcgagaaata cctcggcacc gtcgtaccct acactgacaa cttcttcgct
     2701 acgctcaact cggcggtgtt ctccgacggc tcgttcgtct acgtgccgaa gggcgtgcgc
     2761 tgtccgatgg agctctcgac ctacttccgc atcaatgcgg cgaagaccgg ccagttcgag
     2821 cgcacgctga tcatcgccga cgagggctcg cacgtctcct acctcgaagg ctgcacggcg
     2881 ccgatgcgcg acgagaacca gctgcacgcc gcggtggtcg agctgatcgc gctcgatgac
     2941 gccaagatca agtattccac cgtgcagaac tggtatccgg gcgacgagaa cggcgtcggc
     3001 ggcatctaca acttcgtcac caagcgcggc gagtgccgcg ggcgcaactc gcacatctcc
     3061 tggacacagg tcgagaccgg ctcggcgatc acctggaaat acccgagctg cgtgctgcgc
     3121 ggcgcgggct cggtcggcga gttctactcg gttgcgaccg cgaccaacta ccagcaggcg
     3181 gataccggca ccaagatgat ccacatcggc gccgatacgc gcagcacgat cgtctccaag
     3241 ggcatctcgg cgggccacgg ccagaacacc taccgcggcc tcgtccggat catgccgggc
     3301 gcgcacggcg cgcgcaattt cacgcaatgc gattcgctgc tgatgggcgg cgagtgcggc
     3361 gcgcacactt tcccgtacgt cgaagtgaag aacgcgacgg caacggtgga gcacgaagcc
     3421 agcacctcga agatcagcga agaccagctg ttctactgcc tgcagcgcgg catctcgcag
     3481 gaagacgcgg tcaacatgat cgtcagcggc ttctgcaaga gcgtattcaa ggagctgccg
     3541 atggagttcg cggtcgaggc gcagaacctg ttggcggtga cactcgaagg gtcggtgggc
     3601 tgaacatgct gaagatcaat gacctgcacg tcacggtggg ccatcgcaag gtgctcgacg
     3661 ggctgacgct cgaagtgccg accggcgagg tgcacgcaat catgggcccg aacggctcgg
     3721 gcaagagcac gctcgcctat gcgctggcag gcaggcccgg ctacgaggcg gaaagcggca
     3781 gcgtgcagtt cgatggccag gacctgctcg cgatgcccgc ggaggagcgg gcacgcgccg
     3841 gcgtgttcct cggcttccag tacccggtgg agatccccgg cgtcaacaat gtctacctgc
     3901 tgaaggctgc gctcaatgcc cgccgcaagg ctgccggcct gcccgaggtc gacgctttcg
     3961 agttcctcga actcgtgcgc ggcaagatga agttcatgca gatggacgag ggcttcctgt
     4021 cgcgcggcgt caacgagggt ttctccggcg gcgagaagaa gcgcaacgag atcctgcaga
     4081 tgctggtgct cgagccgcgg ctcgcgctgc tcgacgagac cgattccggc ctcgatatcg
     4141 atgccatgaa ggtcgtcgcg cgcggcatcg aatcgctgcg cggccccgag cgttcgatcg
     4201 tgctcgtgac ccactaccag cggctgctcg atcatgtgcg ccccgatcgt gtgcacgtac
     4261 tctcgcgagg acgcatcgtc agaagcggcg acgcggcact cgcgctcgaa ctcgagcgcc
     4321 gcggctacga ctgggtgctg ggggccgacg ccgcatgagc gcgatcacgc aactcgcctc
     4381 ggaccacgag gcggtgctcg cggcgctgcc ggcgggtgag gtttcgcacg cggcgcggcg
     4441 cgccacgctc gagatgctcg ccgagcaggg cctgcccggc ccgcgcgagg agaactggcg
     4501 ctacgccaac ctgcgcccgc tcgagcagct gcgcttcacg ccgcgtctca cgcccgatgc
     4561 cgccgcgctg gcgcaggcca cggagctgct ccctgcgccg ctcgcgggat tcgatcgcta
     4621 cgtcgtcgtc gacgggctgt tcgctgcgcc actgtccgcg ccgaccggca ctgcggggga
     4681 tgtcgaggtc gcgttgcagg caggcatcga tgccggctac ggcagtgcgg gcggagatgc
     4741 gcgcttcggc gcgctcgcga gcgcgttcgc accgcaggcg atccgggttg ccgccggggc
     4801 gggagccgcc cgcgatatcg aagtggtgtt cgtggccacc gccgccggca gcgaggccgc
     4861 ttcctaccct gccctgctgg tgaagctcgc cgcccaggcg cacctgcgcc tcgtcgagcg
     4921 tcacatcggg gcctgcgacg cggcgacttt cgccaacgcg gcgctggcca tcgaggtcgg
     4981 tgcggccggg caactgcggc acgtgcgcgt gcagcaggcc ggcgcgcagg ccacgcattt
     5041 cgagacgctg cgcgccgaag tggcggacag cgcggagtac ctgctcgata ccgtcgcgct
     5101 cggtgcgcaa tcggcgcggt cgactaccca cgcgcggctc gcgggccgcg gcgcgcgcat
     5161 cgcctacagc cgctcggtag cggtcgacgc cacgcgggtg aatgacgccg tggtcgtgat
     5221 cgagcatgcc gctccggcca ccatcacggc gcaggactat cgcggcatcg ccgccggtcg
     5281 cgcgcgcgtc gccttcaacg gccacgtcat catgcgagcg aatgccgcgg gttccgcctc
     5341 gagccagtcg ctgcgcagcc tgctggccgg accgggcacc gaggccgacg tgcgaccgca
     5401 gctcgagatc tacaccgacg acgtcaaggc gagccacggc gcgacagccg gcaagatcga
     5461 cgaacagatg ctgttctacc tgctctcgcg cggcatcgag cgccgcacgg cacagagcct
     5521 cctgaaatgg gcgttcctca ccgagctcat cgcgcgcatt ccgatcccgg acctgcgcgc
     5581 acaggtcgaa cgcgccttcg cgcagcgctt caccggcgag gatgccgccg tcacccggga
     5641 actcgtgtaa tggtcaccgc ggctgccatc gccacccgcc ggctcgacgc ggcgcgagtg
     5701 cgtgccgatt tcccgatcct cgcccgcaag gtcaacgggc agccgctcgt gtacctcgac
     5761 agcgccgcgt cggcgcagcg cccgcgggca gtcatcgccg cggtcgatca ctacgagacg
     5821 catctgcatg ccaacgtgca tcgcggcgtg cacacgctga gccaggccgc gaccgacgcc
     5881 ttcgagggcg cgcgcgagcg cgtgcgccgc ttcatgaacg cgcgctcgac gaaggagatc
     5941 gtcttcacgc gcggcacgac cgaagcgatc aacctggtcg cgcagtcctt cgcacgaccc
     6001 cgcctcgggc cgggcgacga aatcctcatc acctggctcg agcatcacgc caacatcgtg
     6061 ccgtggcaga tggtatgcgc ggccacgggc gccacgctgc gcgtcgcacc gatcgatgag
     6121 cgcggcgagt tgcggttcga cgaattccag gcgctgcttt cccctcgcac gaagctcgtc
     6181 gccaccgcgc acgtctcgaa cg
//
LOCUS       DAOQVY010000135         6171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_14076_length_6171_cov_4.581426, whole genome shotgun sequence.
ACCESSION   DAOQVY010000135 DAOQVY010000000
VERSION     DAOQVY010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14076_length_6171_cov_4.581426"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /gene="murI"
                     /locus_tag="PKN91_10915"
     CDS             complement(<1..677)
                     /gene="murI"
                     /locus_tag="PKN91_10915"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNS28619.1"
                     /translation="MPAHVAKRRTAPIGVFDSGVGGLTVLRALRAALPLEDFIYLGDT
                     ARLPYGTKSGDTVMRYALQASRVLVARSIKCLVVACNTASAVSLPAMRAAFGELPIVG
                     VIEPGASAVCAASRSGRIAVIATDGTVRGGAYETAILARRPDARVTSLACQVFVALAE
                     EGWTSGAVAREAAHRYLADLFERRESERPDAMVLGCTHFPMLIEPIRAAVGEGVAIVD
                     SAATTAAA"
     gene            complement(678..2171)
                     /gene="lysS"
                     /locus_tag="PKN91_10920"
     CDS             complement(678..2171)
                     /gene="lysS"
                     /locus_tag="PKN91_10920"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNS28620.1"
                     /translation="MTIDDTGDENRLIAERRAKLAALREAGPAYPNDFRRDALAAQIE
                     AAYGDKNAEWLEANPVTVRVGGRLMAKRVMGKASFAKLADRTGRIQVFLQRDALGASY
                     EAWKGWDVGDILGAEGQLFRTRTGELSVRASAVRLLAKSLRPLPDKWHGLVDTETRYR
                     QRYVDLIVNDESRAVFVQRIAIIRYLRSFLDAMDFMEVETPMMQPIPGGAAARPFVTH
                     HNALDIDMYLRIAPELYLKRLVVGGFERVYEINRNFRNEGLSTQHNPEFTMLELYWAY
                     ADYRDLMELVERMLRGLADAVAGTRRINYQGRAYDLDQPFRRVTIEQAIIEHNPDIDP
                     LSLRDVTYLRRCCERLGIPVGKHDGAGKLQIEIFEKTAEHTYVDPTFVYAYPAEVSPL
                     SRASDADPFITDRFEFFLAGRELANGFSELNDPEEQAARFRAQALRKEAGDEEAMFYD
                     ADYVRALEYAMPPTAGLGIGIDRLVMFFTDSASIRDVLLFPHMRPET"
     gene            complement(2176..3271)
                     /gene="prfB"
                     /locus_tag="PKN91_10925"
     CDS             complement(join(2176..3198,3200..3271))
                     /gene="prfB"
                     /locus_tag="PKN91_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_133881416.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNS28621.1"
                     /translation="MEVSQLKRTLKDLEARANSLRGFLEYDAKVERLEEVSRELEDPE
                     VWSNPERAQQLGKERARLGADIEQLDRTSHAISDAGELLELAEAESDESAARDIEHEC
                     SALEAEVRKLEFKRMFRGEMDSNNAYIDIQAGAGGTEAQDWAQMLMRMYIRWAAARGF
                     ECEVIDSNPGEVAGVKNATLEVRGEYAYGWLRTETGVHRLVRKSPFDSGNRRHTSFAS
                     VFVSPEVDEDIDIEVNPADIEMDVFRSSGAGGQHVNKTESAVRLRHIPSGIVVACQNE
                     RSQHKNRSTAMKMLKAKLYELEVNKRNAAAKVLEDSKSDVSWGNQIRSYVLDQSRIKD
                     LRTNVEVGNTQSVLDGDLDQFMEASLKAGL"
     gene            complement(3284..5017)
                     /gene="recJ"
                     /locus_tag="PKN91_10930"
     CDS             complement(3284..5017)
                     /gene="recJ"
                     /locus_tag="PKN91_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNS28622.1"
                     /translation="MTLRIERRAVPESAAQSLGVTLHPVLRRVYAARGIGSEAELALS
                     LSRLLPVGSLENVDAAVELLLAHREAGRKVLVVGDFDADGATSSALIVRALRAWGFAD
                     VDFLVPNRFEFGYGLTPEIVALGAERGPTLIVTVDNGVASIAGVAAARERGIDVLVTD
                     HHLPGAELPAANVLVNPNLAGSRFGSRALAGVGVAFYVMVALKRALDARGLVAPGAPG
                     PAEWLDLVALGTVADVVPLDFNNRVLVAQGLARIRAGRCVPGIRALLEIAQRERAELT
                     ASDLGFAIAPRLNAAGRLDDMSIGIRCLLADGEAQARALAARLDQLNTERRVIEARMQ
                     ADALAAVRHLGPLAAESARQRWGVCLYEPRWHQGVVGLVASRVKERVRRPVIAFADGG
                     DGTLRGSARSVPGVHVRDVLENVAARHPQLIVRFGGHAMAAGLTLERDKLDTFAAEFD
                     AEVARWLGAHADADRLDTDGELAADDICLATAEAVRAGGPWGAAFPEPVFDGYFSIAR
                     TRIVGEKHVKMWVELAGAARRFDAIAFNLLDGDAAGPPSGEVRLVYRLDVNFYQGERR
                     LQLLVDHVLPA"
     gene            complement(5014..5961)
                     /locus_tag="PKN91_10935"
     CDS             complement(5014..5961)
                     /locus_tag="PKN91_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28623.1"
                     /translation="MPELVLIVPAPSPADRAALTRPLARARPAPLAIGDWRGWLARRL
                     GREDLARQPPAQLAAGRSAPGGDAWFATPVALLAALDHVRMAPDGCVLLAAEQAAQLA
                     ADFAARLGGADAQGARLALQPAGAEGFLLSGLVAQARTHDPARVLGADIGPWLPTGED
                     AAPLRRLGSEIEMWLHEHPLVRAPRPRGAPPVTSLWLWGGGPTVPAQAPARPMAPWPR
                     GYGIDSWLHGLWRAADESLAGHGEEFASISVTPDRDTVVVVRGDEPIASAWLAPALDA
                     LAARRVRRVRLVIDERVFGFSRFDLVKPWRRAVEWTVPA"
BASE COUNT          954 a         2179 c         2112 g          926 t
ORIGIN      
        1 gccgcagccg tggtcgccgc cgaatcgacg atcgcgacgc cctccccgac cgccgcgcgg
       61 atcggttcga tcagcatcgg gaagtgggtg cagccgagca ccatcgcatc gggccgctcg
      121 gactcgcgtc gctcgaacag atcggcgagg tagcgatgtg cggcctcgcg cgccaccgcg
      181 ccactcgtcc atccctcttc tgcgagcgca acgaagacct ggcaggcgag cgacgtgacg
      241 cgcgcatcgg ggcgccgcgc aagaattgcc gtttcgtagg caccgccgcg caccgtgcca
      301 tcggtggcaa tgaccgcgat gcggccgctg cgcgatgcgg cgcatacggc ggacgcgccc
      361 ggctcgatca cgccgacgat cggcagttcg ccgaaggccg cgcgcatcgc cggcagcgat
      421 accgccgagg cggtgttgca ggcgaccacg aggcatttga tcgaacgcgc gaccagcact
      481 cgtgacgcct gcagcgcata gcgcatcacc gtgtcgccgc tcttggtgcc atagggcagc
      541 cgcgcggtat cgcccaggta tatgaagtcc tccagcggca gcgccgcgcg caatgcgcgc
      601 agcacggtga ggccgccgac accggagtcg aatacgccga tcggcgccgt gcggcgcttg
      661 gccacgtggg caggcactca ggtttccggg cgcatgtgcg ggaacagcag cacgtcgcgg
      721 atcgacgccg agtcggtgaa gaacatcacg agccggtcga tgccgatgcc gaggcccgca
      781 gtcggcggca ttgcgtactc gagcgcgcgc acgtagtcgg cgtcgtagaa catcgcctcc
      841 tcgtcgcccg cttccttgcg caacgcctgg gcgcgaaagc gcgcggcctg ctcctcggga
      901 tcgttcagct cggagaaacc attcgcaagc tcgcggccgg cgaggaagaa ctcgaagcga
      961 tcggtgatga aaggatccgc atcgctcgcg cgcgacagcg gcgacacctc cgccggatag
     1021 gcgtagacga aagtcggatc gacataggtg tgctcggcgg tcttctcgaa gatctcgatc
     1081 tgcagcttgc cggcgccatc gtgcttgccg accgggatgc cgagccgctc gcagcagcga
     1141 cgcaggtagg tcacgtcgcg cagcgacagc gggtcgatat cgggattgtg ctcgatgatc
     1201 gcctgctcga tcgtcacgcg ccggaatggc tggtcgaggt cgtaggcgcg gccctggtag
     1261 ttgatccgcc gcgtgccggc gaccgcatcg gccaggccgc gcagcatgcg ctcgaccagc
     1321 tccatcaggt cgcggtagtc cgcataggcc cagtagagct cgagcatcgt gaactcgggg
     1381 ttgtgctggg tcgacaggcc ctcgttcctg aaattgcggt tgatctcata gacccgctcg
     1441 aagccgccga ccaccaggcg cttcaggtac agttccggcg cgatcctcag gtacatgtcg
     1501 atgtcgagcg cgttgtgatg ggtcacgaac ggtcgcgccg ccgcgccgcc gggaatcggc
     1561 tgcatcatcg gcgtttcgac ttccatgaag tccatcgcgt cgaggaacga gcgcaggtag
     1621 cggatgatcg cgatacgctg cacgaacacc gcgcggctct cgtcgttgac gatcaggtcg
     1681 acataacgct ggcggtagcg cgtctcggta tcgacgaggc cgtgccattt gtcgggcagc
     1741 ggccgcagcg acttcgcgag caggcgcacg gcactcgcgc gcaccgacag ctcgcccgtc
     1801 ctggtgcgaa agagctgccc ctcggcgccg aggatgtcgc caacgtccca gcccttccac
     1861 gcctcgtagc ttgcgccgag cgcatcgcgc tgcaggaaca cctggatgcg cccggtgcgg
     1921 tcggcgagct tcgcgaagct cgccttgccc atcacccgct tcgccatcag gcgtccgccg
     1981 acgcgcacgg tcaccgggtt cgcttccagc cattcggcgt tcttgtcgcc ataggcggcc
     2041 tcgatctgcg cggcgagcgc gtcgcgccgg aaatcgttcg gataggcggg ccccgcctca
     2101 cgcagcgccg cgagctttgc gcgccgctcg gcgatcagcc tgttctcgtc accggtgtcg
     2161 tcgatcgtca cgctgctaca gccccgcctt gagtgatgct tccatgaact ggtcgagatc
     2221 gccgtcgagc accgactggg tgttgccgac ctcgacattg gtgcgcaggt ccttgatgcg
     2281 cgactggtcg agcacatagg agcgtatctg gttgccccag cttacgtccg acttcgaatc
     2341 ctcgagcacc ttggccgccg cgttgcgctt gttgacctcg agctcgtaga gcttggcctt
     2401 cagcatcttc atggcggtcg agcggttctt gtgctggctg cgctcgttct ggcaggcgac
     2461 gacgataccg ctcgggatgt ggcgcaggcg caccgccgat tcggtcttgt tgacgtgctg
     2521 gccaccggcc ccgctcgagc ggaacacgtc catttcgatg tcggccggat tcacctcgat
     2581 gtcgatatcc tcgtccacct cgggggatac gaacacactc gcgaacgaag tatggcgccg
     2641 gttgcccgag tcgaacggcg atttgcgcac caggcggtgc acgcccgtct cggtgcgcag
     2701 ccagccgtag gcgtactcgc cgcgcacttc gagggtggca ttcttgacgc ccgccacttc
     2761 gccggggttc gagtcgatga cttcgcactc gaaaccacgc gccgcggccc agcggatgta
     2821 catgcgcatc agcatctgcg cccagtcctg cgcctcggtg ccgccggcgc cggcctggat
     2881 gtcgatgtag gcgttgttcg agtccatctc gccacggaac atgcgcttga actcgagctt
     2941 gcggacctcg gcctcgagcg cactgcactc gtgttcgatg tcgcgggcgg cgctctcgtc
     3001 ggactcggcc tcggccagct cgaggagctc gccggcgtca ctgatggcat gactcgtgcg
     3061 gtcgagctgt tcgatgtcgg cgccgagcct cgcgcgttcc ttgccgagct gctgcgcgcg
     3121 ctcgggattc gaccagacct cggggtcctc gagttcgcgg cttacttctt cgagacgctc
     3181 gaccttggcg tcgtattcaa agaaaccccc gtaacgaatt cgcacgcgcc tcgaggtcct
     3241 tgagcgtgcg cttcagctgg ctgacttcca tcgtggaatt ttatcaggcg ggcagcacgt
     3301 ggtcgaccag caactgcagt cgccgctcgc cctggtagaa gttcacgtcg agacgataga
     3361 cgaggcgcac ctcgccgctg ggcgggccgg ccgcgtcgcc atcgagcagg ttgaaggcga
     3421 tggcgtcgaa gcggcgcgcc gcgcccgcca gctcgaccca catcttcacg tgtttctcgc
     3481 cgacgatgcg cgtgcgggca atcgagaagt aaccgtcgaa cacgggttcc ggaaacgccg
     3541 cgccccacgg gccgcccgcg cggacggcct cggccgtggc gaggcagatg tcgtcggccg
     3601 cgagctcacc gtccgtgtcg agccgatcgg cgtcggcatg cgcaccgagc cagcgcgcga
     3661 cctcggcatc gaactcggcg gcgaaggtat cgagcttgtc gcgctcgagg gtcaggccgg
     3721 ccgccatggc gtgcccgccg aagcgcacga tcagctgcgg gtggcgcgcc gcgacgttct
     3781 cgagcacgtc gcgcacgtgc acgcccggca ccgagcgcgc cgagccgcgc agcgtgccgt
     3841 cgccaccgtc ggcgaatgcg atgaccggcc ggcgcacgcg ctccttgacg cgactcgcca
     3901 cgagtccgac tacgccctgg tgccagcgcg gctcgtagag gcataccccc catcgctggc
     3961 gcgcgctctc ggcggccagc ggcccgaggt gacggaccgc ggcgagcgcg tcggcctgca
     4021 tgcgtgcctc gatcacacgc cgctcggtgt tcaactggtc gaggcgtgca gcgagtgcgc
     4081 gcgcctgcgc ctcgccatcc gcgagcaggc agcggatgcc gatcgacata tcgtcgagcc
     4141 ggccggcggc attgagccgc ggcgcgatcg cgaacccgag gtcgctcgcg gtgagctcgg
     4201 cgcgttcgcg ctgcgcgatc tcgagcagcg cgcggatgcc cggcacgcag cgcccggcgc
     4261 gtatgcgcgc gagaccctgc gccaccagca cgcgattgtt gaaatcgagc ggcacgacgt
     4321 cggccaccgt gccgagtgcc acgagatcga gccattcggc cggcccgggc gcgccgggcg
     4381 cgacgagccc ccgcgcatcg agcgcgcgct tgagcgccac catcacgtag aacgccacgc
     4441 cgactccggc gagcgcgcgg ctgccgaagc ggctgccggc gagattggga ttgacgagca
     4501 cattggccgc gggcagttcg gcgccgggca ggtgatggtc ggtgacgagc acatcgatgc
     4561 cgcgctcgcg cgccgccgca acgccggcga tgctcgcgac gccgttgtcg accgtgacga
     4621 tcagcgtggg gccccgttcg gcgccgagcg ccacgatctc cggcgtgagg ccgtagccga
     4681 actcgaagcg gttcggcacg aggaagtcga cgtccgcgaa tccccaggcg cgcagcgccc
     4741 gcacgatcag cgccgaactc gtcgcgccgt cggcatcgaa gtcgccgacc acgagcacct
     4801 tgcgccctgc ttcccggtgg gcgagcagca gttctacggc ggcatccacg ttctcgagcg
     4861 aacccaccgg cagcagccgc gacaggctga gcgcgagctc ggcctcgctg ccgatgccgc
     4921 gcgctgcgta cacgcggcgc agaacgggat gcagcgtgac gccgagcgat tgcgctgcgg
     4981 attccggtac cgcgcgtcgc tcgatgcgga gagtcatgcc ggcaccgtcc actcgaccgc
     5041 gcgccgccag ggcttcacca gatcgaagcg cgagaagccg aagacgcgct cgtcgatgac
     5101 gagccgtacg cggcgcaccc gccgcgcagc gagcgcatcg agcgccgggg caagccaggc
     5161 cgatgcgatc ggctcatcgc cgcgtaccac gacgaccgta tcgcgatccg gcgtcacgct
     5221 gatcgaggcg aattcctcac catggccggc cagcgactcg tcggccgccc gccacagccc
     5281 gtgcaaccag ctgtcgatgc catagccccg cggccacggg gccatgggcc tcgcgggcgc
     5341 ctgcgcgggc acggtgggcc cgccgcccca caaccacagc gacgtcaccg gcggcgcgcc
     5401 gcggggacgc ggcgcacgta cgagcggatg ctcatgcagc cacatctcga tctcgctgcc
     5461 gagccggcgc agcggggctg cgtcctcgcc cgtcggcagc caggggccga tatccgcacc
     5521 gagcacgcgc gccggatcgt gggtgcgcgc ctgtgcgacg agtcccgaga gcaggaaccc
     5581 ctcggcgccg gcgggctgca gcgcaagccg cgcgccctgc gcgtccgcgc cgccgagtcg
     5641 cgccgcgaaa tccgcggcga gctgcgcggc ctgctccgct gccagcagca cgcaaccgtc
     5701 cggcgccatg cgcacgtgat cgagtgcggc gaggagcgcg accggcgtgg cgaaccaggc
     5761 gtcgcccccg ggagcgctgc ggcccgcggc aagctgcgcc ggcggttgcc gcgcgagatc
     5821 ctcgcgcccg aggcgccgcg caagccagcc gcgccagtcg ccaatcgcga gcggcgcggg
     5881 ccgcgcacgc gccagcggcc gcgtgagggc ggcacgatcg gcaggcgacg gtgcgggcac
     5941 gatgaggacg agttcaggca cgatcgcgta tcgtaccgaa gtcacctgcc gggcgaccga
     6001 cgatgaaact cacgattgac tcgcgcgccg acatcaacct gatccgcagc cacgcgccgg
     6061 gcgaagtgcg cgtcggcgat gcgttgctgc gcgcgcctgt gctgatcagc gcgacggcga
     6121 tcgttgccga ctggccagcg cgctcgattg ccgcgctcga cgaggcgcaa c
//
LOCUS       DAOQVY010000136         6120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_14234_length_6120_cov_5.659522, whole genome shotgun sequence.
ACCESSION   DAOQVY010000136 DAOQVY010000000
VERSION     DAOQVY010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14234_length_6120_cov_5.659522"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            744..959
                     /locus_tag="PKN91_10940"
     CDS             744..959
                     /locus_tag="PKN91_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS28624.1"
                     /translation="MDDRWLSVDEIADYLGVAKDTIYTWVTSKGMPGHKVGRFWKFKK
                     QDVDAWVREGGAASSSDDFDDKEPRNV"
     gene            952..2994
                     /locus_tag="PKN91_10945"
     CDS             952..2994
                     /locus_tag="PKN91_10945"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNS28625.1"
                     /translation="MSKPRSTQDNNDMSNPDQETQAPESALEEGKLFDYITGKSVKDT
                     DKEKVRQRIARALIHEYGIAAEDIEPDFKLKVLGKNRKVDIAIFKPGQPHTEDNLYRA
                     VVVEKEPKIGTKGAYRMRDPEEARKEFELLEAVMAEVESCDYGLWTNGLEFFFFKKEI
                     TRFDTKFKPIGDWPLGDDTFSVEGRSMGRLRRAEPDMLRIAFRRCHNYIHGNEGMPKD
                     AAFWQFLYLIFCKMYDEQQPNEARQFWVGAFEPFDPAGRERIRKRIKPLFEAVKKKYD
                     SLFKGNEEITLSDRALAFIVSELARYDFGRTDVDAKGTAYQEIVGTNLRGDRGQYFTP
                     RGAIRLVVQMLAPQEHERVLDSSCGTGGFLVETLNYMNKVFHDERHIKSGEEDTEEFI
                     SIRDRLANYAAKNLFGADFDPFLVRAAQMNVMMAGNSLGHLYHMNSLEFPRGDLPGVQ
                     PANDNIPLGSIDVLMTNPPFGSDIPVTERTILEQYELARRWERQGDGFVMTNAIKPAV
                     SPEVLFIERCVKWLKPGGRTGIVLPDGILGNPGDEYIRYWILRHCWVLASIDLPVESF
                     IVEANVNILTSLLFLKRKPDEVIQAEDLGQKQDYPVFMAVAEKVGFDRRGNTLYKRHP
                     DGEEILRDVSVEEKVRIGGRLEIRTLQRKERILDDDLPEIAKAYTAFRAQHPEPGK"
     gene            2991..4328
                     /locus_tag="PKN91_10950"
     CDS             2991..4328
                     /locus_tag="PKN91_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS28626.1"
                     /translation="MITRLEATRYRCFERLGIDVGDFRVLVGANGSGKTTLLDLPVLL
                     GDLLRANNVAIPFMERRQELPPRAGSLNELVFAGRGSDFALAVEARLPDVVQSKVLEG
                     MFARKRTERSRQALQEDRRQWPTHIRYEIGLRISSDQALQVAYEYLFLFPESDAPDRR
                     QAGFHGTAAESKKHWHLTIRREFGYESEFKPETPQANPAKVGLPDTLLAMPRVLFEPE
                     AGYPAARWLFGLLTSDAVFLEPNWAAMRQASPPGQPKVITANGRNIPWLALELKREGA
                     APGAPDRYRSERYLDWIAHVQTALPQIVDIDVHERPDDHHAYFFVSYRGDFQVPSAGL
                     SEGTLRILTLTLLPYLSKQPAILVTEEPENGIHPRAIEAVLQSLSSMYDSQVWVSSHS
                     PVVLARAKLDQLLCARLASEGGVEMVPGSEHPRLQEWRGGIDLGSLFAAGVLG"
     gene            4325..4981
                     /locus_tag="PKN91_10955"
     CDS             4325..4981
                     /locus_tag="PKN91_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28627.1"
                     /translation="MISKRPCVILVADSNMAAAFRGYFQRDGWHFSLGCAPFEIDTGV
                     GADLIVDEGGNDPGVYTKGHELLRPYQASHHHALVVLDCEWEGSPGKAAIEAHITERL
                     VDSGWAAESVKVIAIDPELENWLWQDKPQVAEALRYTGQPPLRQLLADKGWWPQGAAK
                     PLRPKEAAEWVLRQTRQPRSSSIYQKLAAHISIKGCTDATFIELHAALQAWFPQEEPA
                     "
     gene            4978..>6120
                     /locus_tag="PKN91_10960"
     CDS             4978..>6120
                     /locus_tag="PKN91_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28628.1"
                     /translation="MSSLKWKAVRAAWLEEGGRRLDCNPYMSGALEARDTLKQLKTRK
                     DLLRNLTAGYAGGIYNGPVFARVWVDDPRYGVPFLSNSDMLNADLSNLPLLQRRYAQS
                     KKLAHLELAAGTTLITCSGTIGRMTYVRPDMAGIWSSQHIMKVVPNPERIPPGYLYAF
                     LSSRYGVPLVVSGTYGAIIQHIEPEHIADLPVPRFDPKIEEVIATKVDAASQARAQAV
                     TLLRDAARRLHNTLGLQAPTPIAALPKPDVTSVSSNTFQDRGDAYYYGARNAESRAAF
                     DRLTVNRQLGEVAEVFIPGIFKRLYASDPQYGSPYITGGDVFELAPTSDKFLMTRVAA
                     EYRLLLKQGMIVVQEAGQLGGLIGRSVMVGRHLDGFSCSNNMIRIVP"
BASE COUNT         1244 a         1938 c         1821 g         1117 t
ORIGIN      
        1 ctgaccccca gccagcgcga ggcgctcggc aagttcgtcc ccaatccctt cccgcttgcc
       61 cacactagcg cgcccgttcg ccacaagtcg agcgaccccc tgccgatccg cccaagttgg
      121 ctgggctggc gttgagccgg ggcacacgag gaccttcatg tccttgacga gttgatggtg
      181 gagttggagc cctaacgccg ggagtgtgga ttggcaccct accctttcag gccaggcgac
      241 tcccttcctc gttccaatag gtgggtggga tcgagccttg acgcgctggg cggtgcactt
      301 aatcgtggaa gaggtctttg agcgtgttgc gaagcgacag aggggagtct tttggtgacg
      361 aactcggatc gcacacaaac gaaaccccct cgctttcagc cgcaaatctc tgacggtcga
      421 tcctgtcctt ggcccgccac ttggcaagcg agcttccctg caggttttct gtatcgattt
      481 caacggactg cgccggctca cgagctgttc cgccagattc ggctggcgga gcggattaaa
      541 taaccgcgtc cttggtgcgc acgctttcac tgctgcgacg gccccaatgt ctagtgtgaa
      601 gcccccacgc taatgacttc agctccaact cacattaagc gccccagccc catccaaacc
      661 caatctaacc caaccaagcc cagccagagt caggtatcct gaggcgcatc ccaaaccgca
      721 tactgcaagc tttggagccc cacatggacg accgctggct ctccgtcgac gaaatcgccg
      781 actacctcgg cgtggccaag gacacgatct acacctgggt gacctccaag ggcatgcctg
      841 ggcacaaggt cgggcgcttc tggaagttca agaagcaaga cgtggacgcc tgggtacgag
      901 agggcggcgc cgcctccagc agcgatgact tcgacgacaa ggagccgcgc aatgtctaag
      961 ccgcgctcca cgcaggacaa caacgacatg agcaacccgg atcaagagac ccaagcgccg
     1021 gaaagcgcgc tcgaagaggg caagctcttc gactacatca ccggcaagtc ggtgaaagac
     1081 accgacaagg agaaggttcg gcagcgtatc gctcgcgctc tcatccacga atacggtatc
     1141 gccgccgaag acatcgagcc cgatttcaag ctcaaggtgc tcggcaaaaa ccgtaaggtc
     1201 gacatcgcca tcttcaagcc ggggcagcca cacacagaag acaacctcta ccgcgcggtg
     1261 gtggtcgaga aggagcccaa gatcggcaca aagggcgcct atcgcatgcg cgacccggaa
     1321 gaggcgcgca aagagttcga gttgctcgaa gccgtaatgg ccgaagtcga aagctgcgac
     1381 tacgggctgt ggaccaacgg cctggagttc ttcttcttca agaaggaaat cacccgcttc
     1441 gacaccaagt tcaaaccgat cggcgactgg cccctgggcg acgacacatt cagcgtcgaa
     1501 ggccgctcca tggggcggct gcgccgagcc gaacccgaca tgctgcgcat cgccttccgg
     1561 cgctgccaca actacatcca cggcaacgag ggcatgccca aggatgcggc gttctggcag
     1621 ttcctctacc tcatcttctg caagatgtac gacgagcagc agccaaacga ggcccggcag
     1681 ttctgggttg gtgcattcga gccgttcgat ccggccgggc gcgaacggat ccgcaagcgc
     1741 atcaagccgc tcttcgaggc cgtcaagaaa aagtacgata gcctcttcaa gggcaacgaa
     1801 gaaatcaccc tgtccgaccg tgccctggcc ttcatcgtct ccgagctggc ccgctacgac
     1861 ttcggccgta ccgacgtcga cgccaaaggc acggcctacc aggagatcgt gggcaccaac
     1921 ctgcgcgggg atcgcggtca gtacttcacg ccgcgtggcg ccatccgcct ggtcgtgcag
     1981 atgctcgcac cgcaagagca tgagcgggta ctggattcat cgtgtggtac cgggggcttc
     2041 ctcgtagaga ccttgaacta catgaacaag gtgttccacg acgagcggca catcaagtcc
     2101 ggcgaggaag acacggagga gttcatctcc atccgtgacc gtctcgccaa ctacgcggct
     2161 aagaacctct tcggtgccga cttcgatcct ttcctggtgc gtgccgcgca gatgaacgtg
     2221 atgatggccg gcaattcgct gggccacctc tatcacatga actcgctgga gtttccgcgt
     2281 ggcgacctgc cgggcgttca gcctgccaat gacaacatcc cgctcggctc catcgacgtc
     2341 ctgatgacga acccgccgtt cggctccgat attcctgtca ccgagcggac catcctcgag
     2401 cagtacgagc tcgcccgccg ctgggagcgg caaggcgatg gcttcgtgat gaccaacgcc
     2461 atcaagcccg ccgtatcgcc cgaagtgctg ttcatcgagc ggtgcgtcaa gtggctcaag
     2521 cccggtggtc gaacaggcat cgtcctgccc gacggcatcc tgggcaatcc gggtgacgag
     2581 tacatccgct actggatcct gcgccactgc tgggtgctgg ccagcatcga cctgccagtg
     2641 gagtcgttca tcgtcgaagc caacgtcaac atcctcacca gcctgctttt cctcaagcgc
     2701 aagcctgacg aagtcatcca ggccgaggac ctcgggcaga agcaggacta tccggtgttc
     2761 atggccgtcg ccgagaaggt gggcttcgac cgccgtggca acaccctcta caagcgccac
     2821 ccggacggtg aggagattct cagggacgtg agcgtggagg aaaaggtgcg catcggcggt
     2881 cggctggaaa tccgtaccct ccagcgcaaa gagcgcatcc tcgatgacga tttgcccgag
     2941 atcgccaagg cttacacggc gttccgcgcc caacacccgg agccggggaa atgattacgc
     3001 gactcgaagc cacacgttac cgctgcttcg aacgcctggg catcgacgtg ggcgactttc
     3061 gggtactggt gggagccaac ggttccggca aaacaacgct gctcgatctg cccgtgcttc
     3121 tgggtgatct gctgcgcgcg aacaatgtcg ccatcccctt catggagcgg cggcaggagc
     3181 taccgccgcg cgctggcagc ctgaacgagc tggtcttcgc agggcgcggc agtgatttcg
     3241 cgctcgccgt tgaagcccgc ctgcccgatg tcgtgcagtc caaggtgttg gagggcatgt
     3301 ttgcccgcaa gcggaccgag cgatcacgcc aagccttgca ggaagaccgc cgccaatggc
     3361 ccacgcacat tcgctatgag atcgggctgc gcatcagttc cgaccaagcg ctgcaagtcg
     3421 cctacgagta tctcttcctg ttccccgaat ctgacgcgcc ggaccgccgt caggcgggct
     3481 ttcacggcac ggcagccgag agcaaaaagc actggcacct caccatcagg cgcgagttcg
     3541 gctacgaatc cgagttcaaa cccgaaacgc cccaagctaa tccggccaag gttggcctgc
     3601 cggacacgct gctcgccatg ccgcgcgtcc tattcgagcc cgaggcaggc tatcccgcgg
     3661 cgcgctggtt gtttggtctg ttgacctccg atgccgtgtt cctggagccc aactgggcgg
     3721 ccatgcgcca agccagcccc ccggggcagc ccaaggtcat cacggccaac ggtcgcaaca
     3781 ttccttggct ggcgctcgaa ctgaagcgcg aaggtgctgc tccgggggcg cccgaccgtt
     3841 atcgcagcga gcgctacctc gactggattg cccacgttca gaccgcactg ccgcagatcg
     3901 tggacatcga tgtgcatgag cgcccggatg accaccatgc ctatttcttc gtgagttacc
     3961 gcggcgattt tcaggtgccc tctgcgggcc tttcagaggg taccttgcgc atcctcacgc
     4021 tgacgctgct gccctatttg agtaagcagc cggccatcct ggtgacggaa gaaccagaga
     4081 acggcatcca cccccgtgcc atcgaagccg tgctgcagtc gctctcgtcc atgtacgaca
     4141 gccaggtatg ggtgtcgtcg cactcgcccg tggtactggc ccgcgccaag ctcgatcaac
     4201 tgctctgcgc ccggctggcc agcgaaggtg gcgtggagat ggtgccgggc agcgaacacc
     4261 cacgcctgca ggaatggcgc ggcggtatcg acctgggcag cctgttcgca gcgggggtgc
     4321 tgggatgatc agcaagcgcc cctgcgtcat cctggttgca gacagcaaca tggcggccgc
     4381 cttccgtggc tatttccagc gcgatggctg gcacttcagc ctcggctgtg cgcctttcga
     4441 gatcgacacc ggtgtcggtg ccgatctgat cgtggacgag ggcggcaacg acccaggcgt
     4501 atacaccaaa gggcacgagt tgctgcggcc ttaccaagcc agccaccacc atgccttggt
     4561 cgtgctcgat tgcgaatggg aaggctcacc gggaaaggcg gccatcgagg cccacattac
     4621 cgaacggctg gtcgacagtg gctgggctgc cgagtcggtc aaggtcatcg ccatcgaccc
     4681 ggagctggag aactggctgt ggcaagacaa accccaggtg gctgaggccc tgcgctacac
     4741 gggccagccg cctttgcggc aactgctggc cgacaaaggc tggtggccac agggtgcagc
     4801 gaagccgctg cggcccaagg aagccgccga atgggtgctc aggcaaaccc gtcagccacg
     4861 ctcatcgtcg atctatcaga agctggcagc gcacatctcg atcaagggct gcaccgatgc
     4921 gaccttcatc gaactgcacg ctgccctgca agcctggttc ccgcaggagg agccggcatg
     4981 agcagcctga agtggaaggc tgtgcgtgcg gcttggcttg aagaaggcgg gcgacggctg
     5041 gactgcaacc cgtatatgtc gggtgcgctg gaagcgcggg ataccctcaa gcaactgaag
     5101 acgcgtaagg atttgctgcg taacctcacg gcaggatatg cgggagggat ttacaacggg
     5161 cctgtttttg cacgcgtctg ggtggatgac ccaagatatg gagttccgtt cttgagcaac
     5221 agcgacatgc tcaatgcgga cctatccaac ctgccgcttc tgcaacggag atatgcccaa
     5281 tcgaaaaagc tggcccatct agagcttgcc gccggtacaa ccttgatcac ctgctcggga
     5341 acgattgggc ggatgaccta cgttcgcccc gatatggctg gtatatggtc atctcagcac
     5401 atcatgaagg tcgttccgaa tcccgagcgc attccaccgg gctacttata cgccttcttg
     5461 tctagccgct atggcgtgcc gctggtggtg tcgggcacct atggcgccat cattcagcac
     5521 atcgaacccg aacacatagc agatttgccg gtgccaaggt ttgatcccaa gattgaggaa
     5581 gtaatcgcca ccaaggtaga tgcggcttcg caagcgcgtg cccaagctgt cactctgttg
     5641 cgtgacgcag ctcggcgctt gcacaacaca ttggggcttc aagcacccac gccgattgct
     5701 gcactaccca agccagatgt tacctcagtg tccagtaaca ccttccagga ccgcggtgat
     5761 gcgtactact acggggcgag aaacgcagag tctcgcgcgg catttgaccg cctgacggtt
     5821 aatcgtcagc tcggagaagt cgctgaggtc tttattccgg ggattttcaa gcggctgtac
     5881 gcgtccgatc cccagtacgg tagcccatac atcacaggcg gtgatgtgtt cgagttggcc
     5941 cccacatcag acaagttcct catgacacgg gttgctgctg agtaccgctt gctgctcaag
     6001 caaggaatga tcgtggtcca agaggccggt cagcttggcg gcttgattgg gcggtctgtg
     6061 atggtggggc gccacctcga cggcttttcc tgcagcaaca acatgatccg gatcgtccct
//
LOCUS       DAOQVY010000137         5577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_16014_length_5577_cov_5.408729, whole genome shotgun sequence.
ACCESSION   DAOQVY010000137 DAOQVY010000000
VERSION     DAOQVY010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16014_length_5577_cov_5.408729"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PKN91_10965"
     CDS             <1..342
                     /locus_tag="PKN91_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28629.1"
                     /translation="SGAAIALAARPVLGRALGRSGAALAAPGLDGARVSGREIGIIAG
                     NRAAGLGRFFAGFDEPNDGTVAVRETTLAGATDRIVLPVSHTGMLLSPRVARETAQFL
                     RHGRFSLTASS"
     gene            complement(309..2906)
                     /gene="mutS"
                     /locus_tag="PKN91_10970"
     CDS             complement(309..2906)
                     /gene="mutS"
                     /locus_tag="PKN91_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNS28630.1"
                     /translation="MDALAAHTPMMQQYLRIKSQYPDVLLFYRMGDFYELFYDDARRA
                     AGLLDITLTARGQSAGAPIPMAGVPYHSVDGYLARLVRKGESVAICEQIGDPAKSKGP
                     VERQVVRVVTPGTITDEALLEDRRDTLLAAVAFDSSARAGERFGLAWLDLGAGRFTVA
                     QGEGASALAAELERLKPAELLVAEGDGARLGRTPHRTRPPWHFELASASRQLTDQLGT
                     LDLAGFGADELPLAICAAGALLQYVRDTQKSALPHLRALSVEERNDALAIDASTRRNL
                     ELDASLTGRTDATLLAQIDAAVTAMGSRLLRRWLNRPLRDHDLLRARHQAIGELLHSR
                     RYEEVREPLRAIGDIERILARVALRSARPRDLTQLRRAIAALPALRAALAAIDSPLVA
                     RLAADIGEHADVLALLAAAIAEEPAQALREGGVIAAGYDADLDELRTISTDTDAFLLE
                     LERRERERSGIANLKLGYNRVQGFFIEIARAQADRVPADYLRRQTVKSAERFVTPELK
                     GFEDRVLGAREKALARERQLYDEVLTQLIDRLAALQATAAAVAEGDALAALAERALAH
                     RWSAPELTRAPQLAIVGGRHPVVEQCIDAPFVPNDLTLDAKRRMLIITGPNMGGKSTY
                     MRQCALIAILAHMGSYVPAERAVIGPIDRIFSRIGAADDLAGGRSTFMVEMTETANIL
                     HNATAQSLILMDEIGRGTSTFDGLSLAWAVARHLATQVRGYTLFATHYFELTALAQEI
                     DTCANAHLDATGHGDSLVFLHAVKDGPADRSYGIAVAQLAGVPRPVIAAARDYLAELE
                     ALRERHAPTPQRELALTPAARQQPEPSPQALAERAALDLLYALDPDTLTPRAALDALY
                     ELAVKLKRP"
     gene            complement(2978..3856)
                     /gene="purU"
                     /locus_tag="PKN91_10975"
     CDS             complement(2978..3856)
                     /gene="purU"
                     /locus_tag="PKN91_10975"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNS28631.1"
                     /translation="MTRLTETSYVLRLTCPNRPGIVAAVSTFLYQHGCNILDAQQFDD
                     TQSELFFSHIVFNPVDAGGAATIEPLRERFAALARDFAMDWSIEDRARRARVLLLVSK
                     QDHCLADLLYRWHSGELAMDVVGIVANHPRETYAHVTFGAVPFHYLPVTRETRPQQEA
                     QLRTVIRDTRADLVVLARYMQILSDELTRELAGRCINIHHSFLPGFKGARPYHQAYAR
                     GVKVIGATAHYVTADLDEGPIIEQDVERVTHRDGPDDLARRGRDIERRVLARAVRAHI
                     NHRIIVNGHRTIVFPE"
     gene            3917..4429
                     /locus_tag="PKN91_10980"
     CDS             3917..4429
                     /locus_tag="PKN91_10980"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HNS28632.1"
                     /translation="MNCDAALFELARSVGGSLRTAGWRLATAESCTAGWLAKAITDVP
                     GSSDWFDCGYVTYSNEAKQRDLGVPAAVLATHGAVSEAVVRAMAEGALRRAGVDVAVA
                     TSGIAGPDGGMPGKPVGTVWFARAVRRGASIDTVASLQHFPGDREAVRRASVHYALQL
                     IADLASREKP"
     gene            4426..4911
                     /gene="thpR"
                     /locus_tag="PKN91_10985"
     CDS             4426..4911
                     /gene="thpR"
                     /locus_tag="PKN91_10985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNS28633.1"
                     /translation="MTSRRLFFALWPDDGERDALLAASAPLRATLGGRAVPRANLHLT
                     LRFLGEVTADEERAQRAWADTVRHEPIDLRFDRLEWWARPRVLVAAPSVEESVREICP
                     DIKSFRAHVTLVREVPPPPRPSKDWPMSPVTWHCDRFVLVESHGGASYSIVGEWPLYK
                     R"
     gene            4965..>5577
                     /gene="recA"
                     /locus_tag="PKN91_10990"
     CDS             4965..>5577
                     /gene="recA"
                     /locus_tag="PKN91_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNS28634.1"
                     /translation="MEDNRKKALAAALGQIEKQFGKGSVMRLGDASATYDVEAVSTGS
                     LGLDIALGIGGLPRGRVVEIYGPESSGKTTLTLQVIAEVQKAGGTAAFVDAEHALDPV
                     YAGKLGVNVNDLLVSQPDTGEQALEITDMLVRSGAVDIVVVDSVAALTPRAEIEGEMG
                     ELQVGLHARLMSQALRKLTGNIKRSNTIVIFINQIRMKIGVMFG"
BASE COUNT          828 a         1856 c         2014 g          879 t
ORIGIN      
        1 agcggcgccg cgatcgcact cgccgcgcga cccgtcctcg ggcgtgcgct cggacgcagc
       61 ggcgcggcgc tcgccgcgcc gggactcgac ggcgcacgcg tcagcgggcg cgagatcggc
      121 atcatcgcgg gcaaccgcgc tgccggactg ggacgcttct tcgcgggatt cgacgagccg
      181 aacgacggca ccgtcgcggt gcgcgagacc acgctcgcgg gcgccaccga tcgcatcgtc
      241 ctgcccgtga gccacacggg catgctgctg tcaccgcgcg tcgcgcgcga aactgcgcag
      301 ttcctgcgtc acggccgctt cagtttgacc gccagctcat agagcgcatc gagcgcggcg
      361 cgcggcgtca gcgtgtcggg atcgagcgca tagagcagat cgagtgcggc gcgctcggcg
      421 agcgcctgcg gcgagggttc aggctgctgc ctcgccgcgg gcgtcagcgc gagctcgcgc
      481 tgtggcgtcg gcgcatgccg ctcgcgcaag gcctcgagct cggcgagata gtcgcgcgcc
      541 gccgcgatga ccggccgcgg cacaccggcc agctgcgcca ccgcgatgcc atagctgcga
      601 tcggcggggc cgtccttcac ggcatgcagg aacaccagcg agtcgccatg gccggtggcg
      661 tcgagatgcg cgttcgcgca ggtgtcgatt tcctgcgcga gtgcggtcag ctcgaagtag
      721 tgcgtggcga agagcgtgta gccgcgcacc tgggtcgcga ggtgccgcgc gacggcccag
      781 gcgagcgaca ggccatcgaa cgtgctcgtg ccgcggccga tctcgtccat caggatgaga
      841 ctctgcgccg tggcgttgtg caggatgttc gcggtctcgg tcatctccac catgaaggtc
      901 gagcgtccgc ccgccaggtc gtcggccgcg ccgatgcgcg agaagatgcg gtcgatgggg
      961 ccgatcaccg cgcgctcggc cggcacgtag ctgcccatgt gcgcgaggat cgcgatcagc
     1021 gcgcactggc gcatgtacgt ggacttgccg cccatgttcg ggcccgtgat gatcagcatg
     1081 cgccgcttcg catcgagggt gaggtcgttg ggcacgaacg gcgcgtcgat gcactgctcg
     1141 accaccggat gccggccacc gacgatcgca agctgcggtg cgcgggtgag ctcgggcgcg
     1201 ctccagcgat gcgcgagcgc acgctcggcc agggcggcga gtgcatcgcc ctcggcgact
     1261 gccgcggccg tggcctgcaa cgccgcgagc cggtcgatca gctgtgtcag cacctcgtcg
     1321 tagagttgcc gctcgcgggc gagcgccttc tcgcgcgctc ccagcaccct gtcttcgaat
     1381 cccttgagct cgggcgtgac gaagcgctcc gcggacttca cggtctgccg gcgcaggtaa
     1441 tccgccggca cccgatcggc ctgcgcgcgc gcgatctcga tgaagaagcc ctgcacgcgg
     1501 ttgtagccga gtttcaggtt cgcgatgccc gagcgctcgc gttcgcggcg ctcgagttcg
     1561 agcaggaacg cgtcggtgtc ggtggagatc gtgcgcagct cgtcgagatc ggcgtcgtag
     1621 ccggccgcga tcacgccgcc ctcgcgcagc gcctgcgcag gttcctcggc gatcgccgcg
     1681 gcgagcaggg cgagcacgtc ggcgtgctcg ccgatgtcgg ccgccaggcg cgcgacgagc
     1741 ggcgagtcga tcgcggcgag cgcggcgcgc agggcgggga gcgccgcgat cgcgcgccgc
     1801 agttgcgtga ggtcgcgcgg ccgcgccgag cgcaacgcga cacgcgcgag gatgcgctcg
     1861 atatcgccga tcgcgcgcag cggctcgcgc acctcctcgt agcggcgcga gtgcagcagt
     1921 tcgccgatcg cctggtggcg tgcccgcagc aggtcgtggt cgcgcaaggg tcggttcagc
     1981 cagcggcgca gcaggcgcga gcccatcgcg gtgaccgccg cgtcgatctg cgcgagcagc
     2041 gtcgcatccg tgcgtccggt caggctcgcg tcgagttcga ggttgcgacg ggtcgatgcg
     2101 tcgatcgcca gcgcatcgtt gcgctcttcg accgacagtg cgcgcaggtg cggcagggca
     2161 gatttctgcg tgtcgcgaac gtactggagc agcgcgcccg cggcgcagat cgcaagcggc
     2221 agttcgtcgg cgccgaagcc ggcgagatcg agggtgccga gctggtcggt cagctggcgc
     2281 gatgcgctcg cgagctcgaa atgccacggg ggccgggtgc ggtgcggggt cctgccgagc
     2341 cgcgcaccat cgccttcggc gaccagcagt tccgccggct tcagccgctc gagttcggcg
     2401 gcgagcgcac tcgctccctc gccctgtgct accgtgaagc gcccggcgcc gagatcgagc
     2461 caggcgagcc cgaagcgttc accggcgcgc gccgaagagt cgaacgcgac tgccgcgagc
     2521 agcgtgtcgc gccgatcttc gagcagggct tcgtcggtga tggtgccggg cgtgacgacc
     2581 cgcacgacct ggcgttcgac cggacccttc gatttggccg gatcgccgat ctgctcgcag
     2641 atcgcgaccg attcgccctt gcgcacgagg cgcgcgagat agccgtcgac gctgtggtaa
     2701 ggcacgcccg ccatcgggat cggcgccccg gccgactggc cgcgcgcggt cagcgtgatg
     2761 tcgagcaggc ccgcggcgcg gcgggcgtcg tcgtaaaaga gctcgtagaa atcgcccatg
     2821 cgatagaaga gcagcacgtc ggggtattgc gacttgatgc gcaagtactg ctgcatcatg
     2881 ggcgtgtggg cggctagcgc gtccatggcc tgatcatgca ccgggtgaca atcatgcaat
     2941 gcgtcgaaac cggcatatgg cgggttcgat gaaggtttca ttcggggaag acgatcgtgc
     3001 ggtggccgtt gacgatgatg cggtgattga tgtgtgcgcg caccgcgcgt gcgagcaccc
     3061 gacgctcgat gtcgcggccc ctgcgtgcga ggtcgtcggg gccgtcacga tgggtgaccc
     3121 gctccacatc ctgctcgatg atcggccctt cgtcgaggtc cgcggtcacg tagtgcgccg
     3181 tcgcgccgat cactttcacg ccgcgcgcat aggcctggtg gtagggtcgc gcccccttga
     3241 agccgggcag gaagctgtgg tggatgttga tgcagcgacc cgcgagctcg cgcgtcagtt
     3301 cgtcggaaag gatctgcatg tagcgcgcga gcacgacgag gtcggcgcgc gtgtcgcgga
     3361 tcaccgtgcg cagctgcgct tcctgctgcg gccgggtctc gcgcgtgacc ggcagatagt
     3421 ggaagggcac ggcgccgaaa gtcacatgcg cataggtctc gcgcggatgg ttcgcgacga
     3481 tccccaccac gtccatcgcc aactccccgc tgtgccagcg gtacagcagg tcggccaggc
     3541 agtgatcctg cttcgacacg agcagcagca cgcgggcccg gcgcgcccgg tcttcgatcg
     3601 accagtccat cgcgaagtcg cgggcgagtg cggcgaagcg ctcgcgcagc ggctcgatcg
     3661 tcgccgcgcc gccggcgtcg accggattga agacgatatg ggagaagaac agttccgact
     3721 gcgtatcgtc gaactgctgc gcgtcgagga tgttgcagcc gtgctggtac aggaaggtcg
     3781 acacggccgc gacgatgccg ggccggttcg ggcaggtcag gcgaaggacg tacgaggtct
     3841 cggtcaagcg ggtcatcctc tgggcgttcg ccgcccatac taaatgagcg gccggatcgg
     3901 ttagccttgt ccggccatga attgtgacgc agcgctgttc gagcttgccc ggtcggtcgg
     3961 cgggtcgctg cgcacggcag ggtggcggct ggcgacggcc gagtcctgca ccgccggctg
     4021 gctcgccaag gccatcaccg acgtgccggg cagttccgac tggttcgact gtggctacgt
     4081 cacctattcc aacgaggcga agcagcgcga cctgggcgtt cccgcggccg tgctcgccac
     4141 gcacggtgcc gtgagcgagg cggtggtacg ggccatggcg gagggcgccc tgcggcgcgc
     4201 cggcgtcgac gtcgcggtcg cgacgagcgg tatcgccggt ccggacggcg gcatgcccgg
     4261 gaagcccgtc ggtacggtgt ggttcgcgcg cgcggtccgt cgtggcgcca gcatcgacac
     4321 cgtagcgagc ctgcagcatt ttccgggcga cagggaggcg gtgcgccgcg cttcggtgca
     4381 ctacgcgctg cagctgatcg cggatctcgc cagccgcgaa aagccgtgac ctcgcggcgc
     4441 ctgttcttcg cgctctggcc cgacgatggt gagcgcgatg cactgctcgc cgcttccgcg
     4501 ccgctgcgcg cgacgctcgg cgggcgcgcc gtgccgcgtg ccaatcttca cctgacgttg
     4561 cgtttcctgg gtgaggtgac ggccgacgag gaacgcgcgc agcgcgcctg ggcggacacg
     4621 gtcaggcacg agccgatcga cctgcgcttc gaccggctcg agtggtgggc gcgaccccgc
     4681 gtgctcgtcg cggcgccgtc ggttgaggag tcggtgcggg agatttgccc ggacatcaaa
     4741 tccttccgcg cacatgtgac gctggtacgc gaagttccgc cgccgccgcg gccgtcgaag
     4801 gattggccga tgagcccggt gacgtggcac tgcgatcgtt tcgtgctcgt cgaaagtcat
     4861 ggcggcgctt catacagtat cgtcggcgaa tggcccctgt ataagcgatg aacacggaag
     4921 atttgcatct ccagcccgca cgcaactatg gaataatcga ggccatggaa gacaaccgca
     4981 agaaagcgct cgctgctgcg ctcggccaga tcgaaaagca gttcggcaag ggttcggtga
     5041 tgcgcctcgg cgatgcgtcg gccacttacg acgtcgaagc ggtctccacc ggctcgctcg
     5101 gtctcgacat cgcactcggc atcggcggcc tgccgcgcgg ccgcgtcgtc gagatttacg
     5161 gcccggagtc ttccggcaag accacgctca cgctgcaggt catcgccgaa gtgcagaagg
     5221 cgggcggtac cgcggccttc gtcgacgccg agcacgcgct cgatccggtc tatgccggca
     5281 agctcggcgt gaatgtgaac gacctgctcg tctcgcagcc cgacaccggc gagcaggcgc
     5341 tcgagatcac cgacatgctg gtgcgttccg gcgccgtcga catcgtggtc gtcgactcgg
     5401 tcgccgcgct cacgccgcgc gccgaaatcg agggcgagat gggcgaactg caggtcggcc
     5461 tgcatgcccg gctgatgtcg caggcgctgc gcaagctcac cggcaacatc aagcgctcga
     5521 acaccatcgt catcttcatc aaccagatcc gcatgaagat cggcgtcatg ttcggca
//
LOCUS       DAOQVY010000138         5574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_16024_length_5574_cov_6.507701, whole genome shotgun sequence.
ACCESSION   DAOQVY010000138 DAOQVY010000000
VERSION     DAOQVY010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16024_length_5574_cov_6.507701"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PKN91_10995"
     CDS             complement(<1..294)
                     /locus_tag="PKN91_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28635.1"
                     /translation="MPAGIYDDQGGARVAIPRHPDMLQYRAVALQLKCHPVNFATSTA
                     AAREIIAANLLRLERALSGLKIIAGDDVRLVVLPEYVFSGFPIDTTIGEWASRA"
     gene            complement(368..1411)
                     /locus_tag="PKN91_11000"
     CDS             complement(368..1411)
                     /locus_tag="PKN91_11000"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNS28636.1"
                     /translation="MKRRAEPTRPNDTAEGDPADPTGLPRRGAPGVVPADRLDLKLLS
                     RFLAVVEHGSFAKAARAVNLTQQAVAYSIASLEETLGVSLFERDQLGAIPTEHGRRLT
                     HHARALLTEAMRTTASLYALQSATAGSVRVGVSELLSAQIVPVALARLLARFPDIDVT
                     VREGTSSRIFDLLQRGEVDFMVGAPPVSIPLRDNVEQELLFEDFDQVAMRTGHPIASL
                     SAPTLADLQALTWIVPQSREEDYQFLCESFLVAGLEAPRHVIHSDGLAFGINLLLLTD
                     YVSLTSLWLSPQIMNAKSGGLFRTYRVRGLDRKRRAFIRFMRSATLSPAAQSLIEEIR
                     TAACKLRSGDRGD"
     gene            1558..3267
                     /locus_tag="PKN91_11005"
     CDS             1558..3267
                     /locus_tag="PKN91_11005"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNS28637.1"
                     /translation="MLQGHNAASAVRIVLEEYGVRSAFALAGASQSVLIDELDRHGMR
                     IVQTRHETATVGAADGYARVRRNIGVAIINADQGMANAVTGVQSAFEACSPVVVLVGR
                     EPHSWIEPELPVDHDALALLRPVTKWARTCHSAQRLSEYVEIACRKALAGRPGPCVVA
                     YPKDLLTQEVLPGVAAGRPPRAVVRPAPDPAALAEIVSLIDRSERPVIIAGSGAYWSG
                     AGSSLRKFSERFGVPVLMNAMGRGLVPEDNRLGWPWPVAQTAADQADLVIWAGARLSR
                     RFGYGLAPRFQRHVPMVQIDLSAEELSRNRPVDVPLLADVRLSIEQLHAALESNAAPT
                     RDPAWLSEALQGRLRVFLEKAAIPAANVNPYRIGTLLREHLGPDTLLVNDGAVILTRM
                     LGVLRFDGPGSYMDTYPLGSMGMGTPMAIGASIAEQDEAAAQSRSPRRVVLVTGDGSF
                     GFYPSELATAVTEKLRLSIIVANNRSWGNELLMQPPVIGRTLNADLGDINYAAIGQAM
                     GFVGEVVSDGDSLPAALARALASPRPSMLDVQVNETPLEQRELTLLYSDVENTRARHF
                     GGA"
     gene            3429..>5574
                     /locus_tag="PKN91_11010"
     CDS             3429..>5574
                     /locus_tag="PKN91_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS28638.1"
                     /translation="MRLTCVRTVSIAVIALVGYGLNQATASPLAQGEASQQQPDGVLA
                     ALEEVTVTAERRSEAEQSVPMAITALSEGMLERQQIRSVAQLGEVVPNFYIAPNTGTS
                     SAAKIFMRGIGEAESFFTADPPVGMYIDDIYIARQTGAIFDLFDIERLEVLRGPQGTL
                     YGRNSSAGAVKLVSKRPVIGEHQGQVEVAFGRFNSTSLRASGSIPIGATAALQGAVLV
                     RERDGWTKNLATGRDVNDQDVKGARLSLLMEPTERFRALVVADYVRERSTPGYAVPLV
                     LDTSIGDPVPGPEAKTGDFSVTNSDIEQPVNDLDHWGVSTTLEFDVNEDLTLKAIASH
                     RVLENELYLDADGDTYQPLPSRASLYHVYQDQEQYQSSLELQALGKLMDSRLSYVAGI
                     YTFREHNDQDSISVLGLPALYGLPVASAGRIDLTNIARESMTTDSYAAFVTGTLELTD
                     RLSFTTGLRYTSETKDFSNDVMRPDGTAHVACLNQTVTPAVQIRAVPCTAADLALGYS
                     DFINKSAFDKTWTAWTPRFVIDFKLNDSAMTYVSAAKGFKGGTTNGRDVAALRNLYRL
                     IGDMETNWSYEAGLKADWFDRRLRTNVAAFRNEYRGLQASLTTPEGGYGRINSGDVRV
                     NGLEFELAGVPVRGLELTANIGLLDAKYTAWDAALSSCAVYGIDTIPEYLAMPLKVMP
                     DWQYRIGANYSYDTGSRGVFSVGADFNERDEYF"
BASE COUNT         1053 a         1695 c         1794 g         1032 t
ORIGIN      
        1 ggcgcggctc gcccattcgc cgatcgtggt gtcgatcgga aatccggaga aaacgtattc
       61 cggcaacacg acgagccgta catcgtcacc cgcaatgatc ttcaggccgg acaatgcgcg
      121 ctcgagccgc aggaggttcg cggcgatgat ctccctcgcc gccgcagtgc tggtcgcgaa
      181 attgaccggg tggcacttga gctggagcgc gacggctcgg tactgcaaca tgtcgggatg
      241 cctcgggatc gctacacgcg ccccgccttg gtcatcatag atgcccgcgg gcatggcaag
      301 catcaaagta ttcttgccgg tccacagaga actcttgcgg caggggtcgg gtgcggcggc
      361 gcgcgcctca gtcgccccgg tcgccgctac gcagtttgca ggccgccgtg cggatctcct
      421 cgatcaacga ctgcgcggcg ggtgagagcg tcgcgctgcg catgaagcgg atgaacgcgc
      481 gtcgcttgcg atcgagtcca cgcaccctgt aggtacggaa tagtccgccc gacttcgcgt
      541 tcatgatctg cggcgagagc cacagcgatg tcaggctgac gtagtccgtg agcagcagca
      601 gattgatgcc gaacgcgagc ccgtcgctgt ggatcacgtg ccgcggagct tcgagcccgg
      661 cgacgaggaa cgactcgcag agaaactggt aatcctcctc gcgggactgg ggcacgatcc
      721 acgtcagggc ctgcagatcg gccagcgtcg gcgcgctcag gctcgcgatc gggtggccgg
      781 tccgcatcgc gacttggtcg aaatcctcga acagcagctc ctgttcgacg ttgtcgcgca
      841 gcgggatcga caccggcggc gcaccgacca tgaaatcgac ctcgcccctc tgcagcagat
      901 caaagattcg cgagctggtg ccttcgcgca ccgtcacatc gatatcgggg aacctcgcaa
      961 gcaggcgcgc cagcgccacc ggcacgatct gcgcgctcaa caactcgctg actccgacgc
     1021 gcacggaccc cgcggtggcc gactgcagtg cgtacaggct cgccgtggtc cgcatcgcct
     1081 ccgtcagcag cgcgcgggcg tggtgcgtca accgacgccc atgttcggtc ggaatggcgc
     1141 cgagctggtc ccgctcgaac aatgaaacac cgagggtttc ctcgagcgac gcgatcgagt
     1201 aggccacggc ctgctgcgtc aggttgaccg cgcgcgccgc cttggcgaag gacccgtgtt
     1261 cgaccacggc aaggaatcgc gacagcagct tcaggtcgag acgatccgcg ggaacgacac
     1321 ctggtgcacc gcgcctcggc agtcccgtgg ggtcggcggg atcgccttct gccgtgtcgt
     1381 tcggcctggt cggttccgct ctacgcttca cgtcagcagt tccagcatgg gcgcgggctc
     1441 ggacaagaac tccttggcgg gggccaaggg cattctgagt gcgaacaggt ggtcaaaggc
     1501 gtaacactgc cattcgtggc ctcgggccac gcgacgcaac gcgccggagc ctggaagatg
     1561 ctgcaaggtc ataacgccgc aagtgccgta cggatcgtac tcgaagagta cggtgtgcgc
     1621 agtgcgtttg cactggcggg cgcctcgcag tcggtgctga tcgacgaact cgatcgtcac
     1681 ggcatgcgca tcgtgcagac gcggcacgaa actgcaacgg tcggcgccgc ggacggctat
     1741 gcccgcgtgc gacgcaacat tggcgtggcg atcatcaacg ccgaccaggg catggcgaat
     1801 gccgtcaccg gcgtgcaatc ggcattcgag gcctgctcac ccgtcgtcgt gctggtcggc
     1861 agggagccac atagctggat cgagccggaa ctgccggtcg atcacgatgc actcgcgctg
     1921 ttgaggccgg tgacgaagtg ggcacgcacc tgccactcgg cccagcgttt gtccgagtat
     1981 gtggagatcg catgccgcaa ggccttggcc gggcggcccg gcccctgtgt cgtggcctac
     2041 cccaaggatc tgctcacgca ggaggtgctg ccgggcgtag ctgcaggccg gccgccgcgc
     2101 gccgttgttc ggccagcacc ggatccggct gcgctcgccg agattgtcag cctgatcgat
     2161 cgctccgaac gacccgtgat catcgccggc agcggcgcct actggtcggg tgccggcagt
     2221 tcactgcgga agttttcgga gcgctttggc gtgccggtgc tcatgaacgc gatggggcgc
     2281 ggcctggtgc cagaggacaa ccggctcggg tggccctggc cggtggcgca gaccgctgcc
     2341 gaccaggccg atctcgtcat atgggcaggc gcacgcctgt cgcgccgctt cggctacggg
     2401 ctcgcgccac gcttccaacg gcacgtgccg atggtgcaga tcgatctgag cgccgaggaa
     2461 ctctcgcgca accgacccgt ggacgtacca ctgttggccg atgtcaggtt gtcgatcgag
     2521 cagttgcacg ctgcgttgga atcgaacgct gcgccgaccc gcgacccagc ctggctcagc
     2581 gaggcattgc aggggcggct gcgtgtgttc ttggagaagg cggcgattcc tgcggcgaac
     2641 gtcaatccat accggattgg tacgctgcta cgcgagcatc ttggccctga cacgctgctg
     2701 gtcaatgacg gcgcagtcat cctgacccgg atgctcggcg tgctgcgctt cgacgggccc
     2761 ggatcgtaca tggataccta tccgcttggc tcgatgggca tgggaactcc gatggcgatc
     2821 ggcgcgtcga ttgccgagca ggacgaggcc gcagcgcaga gtcggtcacc gcgccgcgtt
     2881 gtactggtta ccggcgacgg gtcgttcggc ttctatccca gcgagttggc caccgcggtg
     2941 accgagaagc tgcggctgtc gatcatcgtg gccaacaacc gctcgtgggg caacgaattg
     3001 ctgatgcagc cgcccgtaat cggccgcacg ctcaacgccg atctcggcga catcaattac
     3061 gccgccatcg gccaggccat gggattcgtc ggcgaggtcg tgagcgacgg cgactcgctg
     3121 ccggcagcgc tggcgcgggc gctcgccagc ccgcggccct ccatgctcga cgtccaggtc
     3181 aacgagacac cgctggagca gcgcgaactg acgctgctct acagcgacgt cgagaacacg
     3241 cgggcgcggc atttcggcgg cgcctgacca gaaattcgcg cgaccgcgat acttgggtat
     3301 ccgtcgtcgt tcaccagccg gctgtgggtg tgcggaacag gggagtaggc atgagcaatg
     3361 tcaagtttgt cgggtgcgct cgagcgtaca gttggcaacc ggtatcaagt ttaaggagtg
     3421 acttctcaat gaggcttacc tgcgtgcgca ccgtgtccat tgctgtgatt gccttggtcg
     3481 ggtacggcct gaatcaggca actgcctccc cgcttgccca gggagaggcg agccaacagc
     3541 aacccgatgg agtactcgcg gcgctcgaag aagtcacggt caccgcggag cgccgctcag
     3601 aggccgagca atccgtgccg atggcgatta ccgcattatc cgaagggatg ctcgaacggc
     3661 agcaaattcg gagcgtcgcc caactcgggg aagtcgttcc caatttctac attgcgccga
     3721 acactggtac gagcagtgca gcgaaaatct tcatgcgtgg cattggcgaa gcagaatcct
     3781 ttttcacagc ggacccgccg gttggcatgt acatcgacga tatctatatc gcgcgccaga
     3841 caggcgcaat atttgacctg tttgacattg agcgactcga ggtgcttcgg ggcccccaag
     3901 gcacattgta cgggcgcaac tcctcggctg gcgctgtaaa actggtatcg aagaggccag
     3961 tcatcggaga gcatcagggc caggtggaag tagcctttgg tcgcttcaat agcacgagtc
     4021 tgcgcgcctc cggcagcatt ccaatcggcg cgacggcggc cctccagggg gcggtgctgg
     4081 tgcgtgagcg tgacggttgg acaaagaatc tggccactgg ccgggatgtg aacgatcagg
     4141 acgtcaaggg ggcgcgtcta tcgctactga tggagcccac ggaaagattc cgcgcgctcg
     4201 tcgtggcgga ctacgtacgc gaacgttcga cgccgggcta cgccgtgcca ctcgtacttg
     4261 acacgtcgat tggcgatccg gttcctggcc ctgaagccaa gactggcgac ttttctgtaa
     4321 cgaactcgga catagagcag ccggtgaacg atctcgacca ttggggagtg tcgacgacgc
     4381 tcgagttcga cgtaaatgag gatctcacgc taaaggcaat cgcctctcat cgcgtactcg
     4441 agaacgagct gtatctggat gccgatggag atacctatca gccgctgcca tcacgagcat
     4501 ccctgtacca cgtgtaccag gatcaggagc agtatcaatc aagcttggaa ctacaggcgc
     4561 tgggcaagct gatggactct cgcttgtcct atgtcgcagg aatctacacc tttcgtgaac
     4621 acaatgatca ggacagcatc agcgtgctcg gcctaccagc gctatacggc ctgcccgtcg
     4681 catctgcagg tcggatcgac ctcacgaata ttgcccgcga gtccatgacg actgattcat
     4741 acgcggcttt cgtgaccggt actctggagc ttactgatcg gctatcgttt acgaccgggt
     4801 tgcgatacac gagcgagact aaggacttta gcaatgatgt aatgcggccg gatggcacgg
     4861 cgcacgtcgc ctgtctgaat cagaccgtga cgcctgctgt gcagattaga gccgtcccct
     4921 gcacggcggc cgaccttgcg cttggataca gcgatttcat caacaagagt gcatttgaca
     4981 agacctggac cgcctggaca ccacggttcg tgatcgactt caagcttaat gattcagcca
     5041 tgacatacgt ttctgcggcg aagggattca agggagggac aaccaatggg cgtgatgtcg
     5101 ctgcgcttcg taatctgtat cgcctgatcg gcgacatgga gaccaactgg agctacgaag
     5161 cgggtttgaa agcggattgg ttcgatcgcc gtcttcgtac gaacgtggcg gcattccgca
     5221 atgagtatcg tggattgcag gcgagcctga ccactcctga aggtggttac ggccggatca
     5281 attccggcga tgtccgtgta aatggactcg agtttgagct ggccggagtt ccggtacggg
     5341 gcctggagct tactgccaac attggcttgc tagacgccaa gtacacagcg tgggacgcgg
     5401 ctctttccag ctgcgcagtg tacggcatcg ataccattcc ggagtatctc gccatgccct
     5461 tgaaggtaat gccagattgg cagtaccgaa tcggagcgaa ctactcatac gataccggga
     5521 gccgcggtgt tttctcagtg ggcgccgatt tcaatgagcg tgacgaatac ttca
//
LOCUS       DAOQVY010000139         5515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_16252_length_5515_cov_5.424359, whole genome shotgun sequence.
ACCESSION   DAOQVY010000139 DAOQVY010000000
VERSION     DAOQVY010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16252_length_5515_cov_5.424359"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1654
                     /gene="ettA"
                     /locus_tag="PKN91_11015"
     CDS             2..1654
                     /gene="ettA"
                     /locus_tag="PKN91_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009392033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNS28639.1"
                     /translation="MLRVSKIVPPKRQIIKDISLSFFPGAKIGVLGLNGSGKSTLLRI
                     MAGVDTQIDGEARPQPGIKIGFLPQEPQLDPANNVRQETVLGLGETFAQLTRFNEISE
                     RFAEEMSADEMTALLEEQAKLQDAIDAAGGWEIERKLEIAADALRLPPWEAKIDKLSG
                     GEKRRVALCKLLLSAPDMLLLDEPTNHLDAESIAWLERYLEDYPGTVVAVTHDRYFLD
                     NVAQWILELDRGHGIPWQGNYSSWLEQKEKRLALEERERDALRKTLERELEWVRQNPK
                     ARQAKSKARLARYEELASREFQERNETNEIYIPPGERLGDLVIEVQGLRKAYGDRLLI
                     DNLSFKLPKGGIVGIIGPNGAGKTTLFRMLTGGEAPDAGEIRVGPTVKLAYVDQSRHS
                     LDDKKSVFEEISGGQDVIRVGNYETAARGYVGRFNFRGAQQQQLIGELSGGERNRVHL
                     AKVLRAGGNVLLLDEPTNDLDIETLRALEDALVAFPGCAVVISHDRWFLDRIATHILA
                     FEGNSEVVWFEGNYQEYVEDYRRRKGADAAQPHRIRYKPLTR"
     gene            1706..3199
                     /gene="hutH"
                     /locus_tag="PKN91_11020"
     CDS             1706..3199
                     /gene="hutH"
                     /locus_tag="PKN91_11020"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNS28640.1"
                     /translation="MTAIALDGRNLTLQLLANDARKAGSFTLDADAHVRLGESARQVA
                     AAAQGEAAVYGINTGFGAFANRRIERSAVAALQVNLVRSHACGVGEPLPDAVVRRMLI
                     LKANSLATGCSGVRPEVVDAILALVNAKVLPIVPRFGSVGASGDLAPLAHLALALIGE
                     GEARCRGRTLRGAEVLRAAGIEPLVLEAKEGLAILNGTQLSCALAIEGLFAAQNLLAS
                     SVAAGALTVEALAGSHAPFDARIHDARGLTGQIEVAHRFRTWLADSEIHRSHTDCDRV
                     QDPYAVRCMPQVLGAFADTLAHARSVLEREINAATDNPLVFGADILSGGNFHAAPIGY
                     VADFMAIATVDAASMSERRIDLLDRRVNPRLTMFMADHPGLESGFMIAHVAAAALASS
                     VKALAHPASVDSIPTSAGQEDHVSMAPWAGEKLLTICERTASVLAIEFLAAAHAIDVM
                     RPLHTSGKLEALHTRIRERVPFRREDHRLDADIAAVTALVQSGALAA"
     gene            complement(3227..>5515)
                     /gene="purL"
                     /locus_tag="PKN91_11025"
                     /gene_synonym="purI"
     CDS             complement(3227..>5515)
                     /gene="purL"
                     /locus_tag="PKN91_11025"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNS28641.1"
                     /translation="EIWCNEAQERYVLAIAPRDLGLFDALCARERAPYAVIGDIDASG
                     RLVVHDRLHGNDVVDMPIDVLLGKPPRMTRDVRTITPRRAPLAAATHVTHAAALREAA
                     LRVLQFPAVADKGFLITIGDRTVGGMVSRDQMVGPWQVPVADVAVTLADYEGVAGEAM
                     AMGERTPVAVLDAPASGRLAVTEAVLNILAADVASLGDIRLSANWMAACGEPGEDAAL
                     YATVRAIGEELCPALGIAIPVGKDSLSMKTAWRDAASGGECAVVAPVSLIVSAFAPVA
                     DVRRTLTPELRRGLDTRLVHIALNGTRRRLGGSIFAQVHGELGDDCPDLDSAPRLADF
                     AAALRALREGGHVLAYHDVSDGGLFTTLVEMAFAGHCGLDIELPAMIATAAEQLFAEE
                     PGVVLQVPVASLDAVIGACRAHGLAGCVSPIGAPAAAMRLRIAVADCVLELPWRELRD
                     AWSRTSHAMRRLRDEPECADEEFAAQCDEADPGLSVALSFDPGEDIAAPFIARGARPR
                     VAVLREQGVNSQIEMGAVLARAGFEAHDVHMTDLQAGVRRLEEFRGLVVCGGFSYGDV
                     LGAGEGWAKSILFNPRVRDAFTQYFARPDTFTLGVCNGCQMLAALKALVPGAAHWPRF
                     VRNRSEQFEGRFSLVEIVPSPSVMLAGMAGSRLPVAVAHGEGRAEFASADAESAFLGA
                     GLTAFRYIDTRGAVAQHYPANPNGSPHGIAAVTSADGRVTLTMPHPERSFRYVQNSWR
                     PQGAGEDSGWMRMFRNARVWVG"
BASE COUNT          899 a         1976 c         1871 g          769 t
ORIGIN      
        1 gatgctgcgc gtgagcaaga tcgttccgcc caagcgtcag atcatcaagg acatttccct
       61 gtccttcttc ccgggcgcca agatcggcgt gctgggcctc aatggttcgg gcaaatcgac
      121 gctgctgcgg atcatggccg gcgtcgacac ccagatcgac ggcgaagcgc gcccgcagcc
      181 cggtatcaag atcggcttcc tgccccagga gccgcagctc gatccggcga acaacgtgcg
      241 ccaggagacc gtgctgggcc tcggcgagac cttcgcgcag ctcacccgct tcaacgaaat
      301 cagcgagagg ttcgccgagg agatgtcggc ggacgaaatg acggcgctgc tcgaggagca
      361 ggcgaagctg caggacgcga tcgacgcggc cggcggctgg gagatcgagc gcaagctgga
      421 aatcgccgcc gatgcgctgc gcctgccgcc gtgggaagcg aagatcgaca agctctcggg
      481 cggcgagaag cggcgcgtgg cgctctgcaa gctgctgctg tcggcgcccg acatgctgct
      541 gctcgatgag ccgaccaacc acctcgacgc cgagtcgatc gcctggctgg agcgctacct
      601 cgaggactac ccgggcaccg tggttgcggt gacgcacgac cgctatttcc tcgacaacgt
      661 cgcccagtgg atcctcgagc tcgatcgcgg ccatggcatt ccgtggcagg gcaactattc
      721 gtcctggctc gagcagaagg aaaagcgcct cgcgctcgag gagcgcgagc gcgatgcgct
      781 caggaagacc ctcgagcgcg agctcgagtg ggtgcgccag aacccgaagg cgcgccaggc
      841 gaagagcaag gcgcgcctcg cgcgttatga ggaactcgcg tcgagggagt tccaggaacg
      901 caacgaaacc aacgagatct acatcccgcc cggcgagcgc ctcggcgacc tggtgatcga
      961 ggtgcagggc ctgcgcaagg cctacggcga caggctgctg atcgacaacc tctcgttcaa
     1021 gctgccgaaa ggcggcatcg tcggcatcat cggcccgaac ggcgccggga agaccacgct
     1081 gtttcgcatg ctgaccggtg gcgaggcgcc ggacgccggc gagatccgcg tcggccccac
     1141 ggtcaagctc gcctatgtcg accagtcgcg gcattccctc gatgacaaaa agagcgtgtt
     1201 cgaggagatc tccggcggcc aggacgtgat ccgcgtcggc aactacgaga ccgccgcacg
     1261 tggctacgtg ggccgcttca atttccgcgg cgcgcagcag cagcagctga tcggggagtt
     1321 gtcgggcggc gagcgcaatc gcgtgcacct ggcgaaggtg ctgcgagcgg gcggcaacgt
     1381 cctgctgctc gacgagccga ccaacgacct cgacatcgag acgctgcgcg ccctcgagga
     1441 cgcattggtc gctttccccg gctgcgccgt ggtcatctcg cacgatcgct ggttcctcga
     1501 ccgcatcgcc acccacatcc tcgccttcga gggcaattcc gaggttgtct ggttcgaggg
     1561 caattaccag gaatacgtcg aggactaccg gcgcaggaag ggtgcggacg ccgcccagcc
     1621 gcatcgcatc cgctacaagc cgctgacgcg ctgagtgggc gcggtcggca gagccggtcg
     1681 gggccgatcg cggtacactt gcacgatgac agccatcgcc ctggacggcc gcaacctcac
     1741 cctccagctg ctcgccaacg atgcgcgcaa ggccggcagc ttcacgctcg acgccgatgc
     1801 gcacgtcaga ctcggcgaat ccgcccggca ggtcgccgcc gccgcccagg gtgaggcggc
     1861 ggtctacggc atcaacaccg ggttcggcgc cttcgccaac cgccgcatcg agcgcagcgc
     1921 cgtggccgcg ctgcaggtca acctcgtgcg cagccacgcc tgcggcgtcg gcgaaccgct
     1981 gccggatgcc gtcgtgcgcc gcatgttgat cctgaaagcc aattcgctcg cgaccggctg
     2041 ctcaggcgtg cgccccgagg tcgtcgacgc gatcctcgcg ctcgtcaacg cgaaggtgct
     2101 gccgatcgtg ccgcgcttcg gttcggtggg cgcctcgggc gaccttgcgc cgctcgcgca
     2161 cctcgcgctc gcgctgatcg gcgaaggcga ggcgcgctgc cgcggccgca cgctgcgcgg
     2221 tgccgaggta ctgcgcgccg cgggcatcga gccgctggtg ctcgaggcga aggagggact
     2281 cgcgatcctg aacggcaccc agctgtcctg cgccctcgcg atcgagggcc tgttcgccgc
     2341 gcagaacctg ctcgcgagtt ccgttgccgc gggcgcgctc acggtcgaag cgctcgccgg
     2401 cagccacgcg ccgttcgacg cgcgcatcca cgacgcgcgc ggcctgaccg gacagatcga
     2461 ggtcgcgcac cgcttccgca cctggctcgc cgacagcgaa atccaccgca gccataccga
     2521 ttgcgaccgc gtgcaggacc cttatgccgt gcgctgcatg ccgcaggtgc tcggcgcctt
     2581 cgccgacacg ctcgcgcatg cgcgctcggt gctcgagcgc gagatcaatg ccgcgaccga
     2641 caatccgctc gtgttcggcg ccgacatcct ctcgggcgga aatttccacg ccgcgccgat
     2701 cggctatgtc gcggatttca tggcgatcgc gaccgtcgat gcggcatcga tgtcggaacg
     2761 gcgcatcgac ctgctcgatc gccgcgtcaa cccgcggctc acgatgttca tggcggacca
     2821 ccccgggctc gagtcgggct tcatgatcgc gcacgtcgcg gcggcggcgc tcgcttcaag
     2881 cgtgaaggcg ctggcgcatc cggcctccgt cgattcgatt ccgacttcgg ccggccagga
     2941 agaccacgtc agcatggcgc cctgggcggg cgagaaactg ctgaccatct gcgagcgcac
     3001 cgcctcggtg ctggcgatcg agttcctggc ggcggcgcat gcgatcgacg tgatgcgccc
     3061 gctgcacacg agcggcaaac tcgaggcgct gcatacccgc atccgcgagc gcgtgccctt
     3121 ccggcgcgaa gaccatcgcc tcgacgccga catcgccgcg gtcaccgcgc tcgtgcagtc
     3181 gggcgccctc gccgcctgag acgcgcccgc ggcgccgcgg gaaccgtcag cccacccaca
     3241 cccgcgcgtt gcggaacatg cgcatccagc cgctgtcttc gccggcgccc tgcggccgcc
     3301 aggagttctg cacgtagcgg aacgaacgct cgggatgcgg catcgtcagg gtcacccgtc
     3361 cgtccgcact cgtgaccgcg gcgatgccgt gcggcgagcc gttgggattg gccggatagt
     3421 gttgcgcaac ggcgccgcgc gtatcgatgt accggaaggc cgtgagcccg gcgccgagga
     3481 aggccgactc ggcgtcggcg gaagcaaatt ccgcgcgacc ctcgccatgc gccaccgcga
     3541 ccggcaggcg cgaacccgcc atgcccgcaa gcatcaccga cggcgacggt acgatctcga
     3601 ccagcgagaa gcggccctcg aactgttcgc tgcgattgcg cacgaagcgt ggccagtgcg
     3661 cagcgcccgg aaccagcgcc ttcaaggccg cgagcatctg gcagccgttg cagacgccga
     3721 gcgtgaacgt gtcgggacgc gcgaaatact gtgtgaacgc atcccgcacc cgcgggttga
     3781 acaggatcga cttggcccag ccttcgcccg cgccgagcac gtcaccgtag gagaagcctc
     3841 cgcagaccac gaggccgcgg aactcctcga ggcggcgcac ccccgcctgc aggtcggtca
     3901 tgtgcacgtc gtgcgcctcg aagccggcgc gggcgagcac cgcgcccatc tcgatctggc
     3961 tgttgacgcc ctgctcgcgc agcaccgcga cacgcggcct cgcgccgcgc gcaatgaacg
     4021 gcgccgcgat gtcctcgccg ggatcgaagc tgagcgccac ggacaggccg gggtccgcct
     4081 cgtcgcattg cgcggcaaat tcctcgtcgg cgcattccgg ctcgtcgcgc aggcggcgca
     4141 tggcatgcga ggtccgtgac caggcatcgc gcagctcgcg ccacggcaat tcgagcacgc
     4201 agtcggccac ggcgatccgc agccgcatcg ctgcggccgg cgcaccgatc gggctcacgc
     4261 agccggcaag accgtgcgcg cgacaggcgc cgatgacggc atcgagcgaa gcgacgggca
     4321 cctgcagcac gacgccgggc tcctctgcga acaactgctc cgccgccgtg gcgatcatcg
     4381 ccggcaattc gatgtcgagg ccgcaatgcc cggcgaacgc catctcgacc agggtcgtga
     4441 acagcccgcc gtcagacacg tcgtggtagg cgagcacgtg gccgccttcg cgcagcgcac
     4501 gcagcgctgc ggcaaaatcc gcgagccgcg gggcggagtc gaggtccggg cagtcgtcgc
     4561 cgagttcgcc gtgcacctgc gcgaagatcg agcccccgag ccggcggcgc gtgccattca
     4621 gtgcgatgtg cacgagtcgc gtatcgaggc cgcgccgcag ctcgggagtc agcgtgcggc
     4681 gcacgtcggc gaccggcgca aacgccgaca cgatcagcga caccggcgcg acgacggcgc
     4741 actccccgcc gctcgcggca tcgcgccacg cggtcttcat cgacagcgag tccttgccga
     4801 ccggtatcgc gataccgagc gccgggcaca gttcctcgcc gatcgcgcgt accgtggcgt
     4861 agagcgccgc gtcctcgccg ggctcgccgc acgcggccat ccagttggcc gacaggcgaa
     4921 tgtcgccgag cgacgccaca tcggcggcga ggatgttgag cacggcctcg gtgaccgcga
     4981 ggcgcccgga ggccggcgca tcgagcaccg caaccggcgt gcgctcgccc atcgccatgg
     5041 cctcgcccgc gacaccctcg taatcggcga gcgtgacggc gacatccgcg accggcacct
     5101 gccacgggcc gaccatctgg tcacggctca ccatgccgcc cacggtgcga tcgccgatgg
     5161 tgatcaggaa gcccttgtcg gcgaccgccg ggaactgcag cacgcgcagt gccgcctcgc
     5221 gcagcgcggc cgcgtgggtc acgtgcgtgg ccgcggcgag cggcgcgcgc cgcggcgtga
     5281 tcgtgcgcac gtcacgcgtc attcgcggcg gcttgccgag cagcacatcg atcggcatgt
     5341 cgacgacgtc gttgccatgc aggcggtcgt gcaccacgag gcggccgctc gcatcgatgt
     5401 cgccgatcac cgcgtaaggt gcacgctcgc gcgcgcagag cgcatcgaac aagccgaggt
     5461 cgcgcggggc gatggcgagc acgtagcgct cctgcgcctc gttgcaccag atttc
//
LOCUS       DAOQVY010000140         5449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_16517_length_5449_cov_4.976826, whole genome shotgun sequence.
ACCESSION   DAOQVY010000140 DAOQVY010000000
VERSION     DAOQVY010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16517_length_5449_cov_4.976826"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="PKN91_11030"
     CDS             complement(<1..1099)
                     /locus_tag="PKN91_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS28642.1"
                     /translation="MYLDLFKLRDLPFRLSPDPQYLYLSRNHSRAKAYMESTIWFTDG
                     FVVITGEIGSGKTTLIETFLRELEQDVVVAQVNQTQVSAIEFLQAVLVQFGFSPFKMK
                     KAELLATLNQFLIEQYAAGRKVLLIVDEAQNLSLRVLEEIRLLSGVETAREKVLRIIL
                     AGQPELNDKLDSRELVQLAQRVRLRFHLTALSQEDTRQYVLHRLEVAGAGGREIFAPE
                     TFPEIYRFTGGTPRLVNTLCDTAMMGAYAADQDTVTREDILNAVEELQWAEYASRTHR
                     ELRSMTGAGRAGERALENTGSRRSDVILAHVTLASEDTVLAEVPLRPGRVIIGRTSDN
                     DIQVDSRFVSRHHCQIVTTAESSKCTRRFVPP"
     gene            complement(1124..2233)
                     /locus_tag="PKN91_11035"
     CDS             complement(1124..2233)
                     /locus_tag="PKN91_11035"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNS28643.1"
                     /translation="MTVGIVGAGQLGRMLALAGYPLGLDFRFLDRAADTPGGQVAPIL
                     VGEFTDSTLLRRLARGTRVVTFDWENVPVAPLRALAGRARLAPPLPALAAAQDRLAEK
                     RLFAKLAVPTNASAAVDSARDLARAFARIGPGVLKTRRLGYDGKGQFRVRSARDLDAA
                     WAALGGAPLIYEQFVPFDYEVSIIGARSARGEIAVYPLNGNVHVDGILRVTRAPFGTP
                     ALARAAARYLKRVLEHFRYTGVLAIEFFVKDGRLLANEMAPRVHNSGHWTIEGAQTSQ
                     FENHLRAILGLPLGSTRARGHAAMVNLIGELPPLATALSEPGLHWHDYGKSARPGRKV
                     GHCTLVEASAGSRDRRVRALLPRLAPGVRVSFPRL"
     gene            complement(2247..2738)
                     /gene="purE"
                     /locus_tag="PKN91_11040"
     CDS             complement(2247..2738)
                     /gene="purE"
                     /locus_tag="PKN91_11040"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNS28644.1"
                     /translation="MTALVGIIMGSSSDWETMRAASDTLAQLGIAHEVRVVSAHRTPD
                     LLFEYAAGAQQRGIKVIIAGAGGAAHLPGMTAAKTTLPVLGVPVQSKALSGLDSLLSI
                     VQMPAGIPVATLAIGAAGATNAALMAAAILAISDPAIDTALKDFRARQTATVLEKGDP
                     RGP"
     gene            complement(2748..3266)
                     /gene="yjgA"
                     /locus_tag="PKN91_11045"
     CDS             complement(2748..3266)
                     /gene="yjgA"
                     /locus_tag="PKN91_11045"
                     /inference="COORDINATES: protein motif:HMM:NF016626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HNS28645.1"
                     /translation="MSDETFDDKPSKSARKRAAHAAQDLGARLIALREPELAALRLPD
                     QLLDAVREARRLTSRAALARQRQYIGKLMRGIDTAPIEAALAARSGAGALDARLQQSA
                     ERWRERLLAEGDRALDEFAAAHAPVDRAQLASLIAAAGNPHASAGARTTASRSLFRTL
                     RAVLCRPADPNC"
     gene            3286..4635
                     /gene="pmbA"
                     /locus_tag="PKN91_11050"
     CDS             3286..4635
                     /gene="pmbA"
                     /locus_tag="PKN91_11050"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HNS28646.1"
                     /translation="MQQRVDGTRIAELKGVISSALEAARRAGATQAEADASVQQGLTV
                     NVRLGAVDTIEYQRDRGLGITVYVGQRKGSASTADLAPAAVAAMVEKAVSIARFTAED
                     PFAGLADPDELAVDIPDLDLDHPWELEPGAAVELARRCEAAGLDLDARITNSEGASIS
                     THRAVHVYGNSHGFLAGYPSSSHSVSCALVAQAGSDMQRDYWYTAARDPDELGDIEAI
                     GRRAGERALGRLGARRLGTRRAPVLFAPDIARGVFGHMVGALRGGNQYRRTSFLLDAA
                     GERILPTFIHIHERPHLARALASSPFDAEGVATRDRELVTAGVLQGYVLNSYAARKLG
                     LRTTGNAGGVHNLIVESDRDALPFEALLARMGRGLVVTELMGQGVNGVTGDYSRGATG
                     FWVEGGAPAFPVEEVTIAGNLRDMYLAIDAVGSDVDTRGALRTGSLLIGEMTIAGEA"
     gene            complement(4632..>5449)
                     /locus_tag="PKN91_11055"
     CDS             complement(4632..>5449)
                     /locus_tag="PKN91_11055"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNS28647.1"
                     /translation="LLVAFLGLAHRAMRSESWQVITQLPLLSRAVHFTTGLEPYVMVT
                     AIFTFVYMLVPNTRVRWQSALIGGISGGVLWAAVGTLFTAFVVYSSRLTIVYAGLAII
                     IATLLWTYFGWLILLIGAQVSFYVQNPEYLRIGLRELRLSSIEVERVSLKIMYLVGRS
                     HATGDTTWTVSSLASELGLPGIAVAQLVATLEGAGLVLATDDERLLPGRDIGHIRLQE
                     ILDTARNQKMGHAPARDVSLPPVDRLTRAIDEAWRSCCQDRTLGQLVAEGSEA"
BASE COUNT          849 a         1873 c         1930 g          797 t
ORIGIN      
        1 cgggcgggac gaaacggcgg gtgcacttcg agctctccgc ggtcgtgacg atctggcagt
       61 ggtggcggct cacgaagcgg ctatcgacct ggatgtcatt gtcggaggtg cgcccgatga
      121 tcacgcgccc ggggcgtaac ggcacctcgg ccagcaccgt gtcctcgctg gcgagcgtca
      181 catgcgcgag gatcacgtcg ctgcggcgtg aaccggtgtt ctcgagcgca cgctcgcccg
      241 cccggccggc cccggtcatc gagcgcagct cgcgatgggt gcgcgaggcg tactcggccc
      301 actgcagttc ctcgacggca ttcaggatgt cctcgcgcgt cacggtgtcc tggtcggcgg
      361 cataggcccc catcatcgcc gtatcgcaca gggtgttcac gaggcgcggg gtgccgccgg
      421 tgaagcggta gatctccggg aaggtctccg gcgcgaagat ctcccggccg ccggcgcccg
      481 cgacttcgag gcggtgcagg acatactggc gggtatcctc ctgcgacagc gcggtgaggt
      541 ggaagcgcag gcgcacgcgc tgcgcgagct gcaccagttc gcgcgaatcg agcttgtcgt
      601 tcagctcggg ctggccggcg aggatgatgc gcagcacctt ctcgcgcgcg gtctcgacgc
      661 ccgacaggag ccgtatctcc tcgagcacgc gcaggctgag gttctgcgcc tcgtccacga
      721 tcagcagcac cttgcgcccg gccgcgtact gctcgatcag gaactggttg agcgtggcga
      781 gcagctccgc cttcttcatc ttgaacggcg agaagccgaa ctgcaccagc accgcctgca
      841 ggaactcgat cgccgagacc tgtgtctggt tgacctgtgc caccaccacg tcctgctcga
      901 gttcacgcag gaaggtctcg atcagcgtag tcttgccgga gccgatctcg cccgtgatca
      961 ccacgaaacc gtcggtgaac cagatcgtcg actccatgta cgccttggcc ctggagtggt
     1021 tccggctcag gtacaggtac tgcggatccg ggctcagccg aaatggcagg tcgcgcagct
     1081 tgaacaggtc gagatacatg gcccgacgat cttacgggaa tcgtcagagg cgcggaaacg
     1141 acacgcgcac acccggcgca agccggggca ataacgcccg gacacggcgg tcgcggctcc
     1201 cggcgctcgc ctcgaccagc gtgcaatggc cgactttccg gcccggacgg gccgatttgc
     1261 cgtaatcatg ccagtgcagg ccgggttccg agagcgccgt cgccaggggc ggtagctcgc
     1321 cgatcaggtt caccatggcc gcgtggccgc gcgcgcgggt gctgcccagc ggcaggccga
     1381 ggatggcgcg caggtggttc tcgaactggc tcgtctgcgc gccctcgatg gtccagtgcc
     1441 cggaattgtg cacgcgcggc gccatttcgt tcgcgagcag ccgcccatcc ttcacgaaga
     1501 actcgatggc cagcacgccg gtgtaacgga aatgctcgag cacgcgcttc aggtagcgcg
     1561 cggcggcgcg tgcgagcgcc ggggtgccga acggtgcgcg cgtgacgcgc aggattccat
     1621 cgacgtgcac gttgccgttg agcgggtaga cggcgatctc gccgcgcgcg ctgcgcgcac
     1681 cgatgatcga cacctcgtag tcgaacggca cgaactgttc gtagatcagc ggcgctccgc
     1741 cgagcgcggc ccaggcggcg tcgaggtcgc gcgcgctgcg caccctgaac tggcccttgc
     1801 cgtcatagcc gagccggcgt gtcttcagga cgccgggacc aatacgcgcg aaggcgcggg
     1861 caaggtcgcg cgcgctatcg accgcggcac tcgcattggt gggcaccgcg agcttcgcga
     1921 acaggcgctt ctccgccagc cggtcctgcg ccgcggcgag cgccggcagc ggcggcgcaa
     1981 gccgcgcgcg ccccgccaat gcgcgcagcg gcgcgaccgg cacgttctcc cagtcgaagg
     2041 tcacgacgcg cgtaccgcgc gcgagtctgc gcagcagggt ggaatcggtg aactcaccga
     2101 cgaggatcgg cgccacctgg ccgccgggcg tgtcggcggc gcgatcgagg aaacggaaat
     2161 cgaggccgag cgggtagccc gcgagcgcga gcatgcggcc gagctgcccc gcgccgacga
     2221 tgccgacggt catgtccggg cggcgatcat gggccgcgcg gatcgccctt ctccagcacg
     2281 gtggcggtct ggcgcgcgcg gaagtccttg agggccgtat cgatcgccgg atccgagatc
     2341 gcgaggatcg ccgccgccat cagtgccgca ttggtcgccc cggcggcacc gatcgcaagc
     2401 gtggcgaccg gaatgccggc cggcatctgc acgatcgaca gcagcgaatc gagaccgctc
     2461 agtgctttcg actgcaccgg cacgccgagc acgggcaggg tggtcttcgc ggcagtcatc
     2521 cccggcaggt gggccgcacc gccggcgccg gcgatgatca ccttgatgcc gcgctgctgc
     2581 gcgcccgccg catactcgaa taacaggtcg ggcgtgcggt gcgcagagac gacgcgcact
     2641 tcgtgcgcga tgccgagctg cgcgagcgtg tcgctggcgg cccgcatcgt ctcccagtcg
     2701 gaagacgacc ccatgatgat gccgacgagt gcagtcatcg cgccattcta gcagttgggg
     2761 tccgcggggc ggcagaggac ggcccgcagc gtgcgaaaca ggctgcggga cgcggtggtg
     2821 cgcgcgccgg cactcgcgtg cggattgccc gcggcggcga tcagcgaggc gagctgcgcg
     2881 cggtcgaccg gggcgtgcgc ggcggcgaat tcgtcgagcg cgcgatcgcc ctccgccagc
     2941 aggcgctcgc gccagcgctc cgcgctctgc tgcagccggg catcgagcgc gccggcgccc
     3001 gagcgggccg cgagcgccgc ctcgatcggc gcggtgtcga tgccgcgcat cagcttgccg
     3061 atgtactggc gctggcgcgc gagcgccgcc cggctcgtca gccgacgcgc ctcgcgcacg
     3121 gcatcgagca actggtcggg aagccggagc gccgcgagct cgggctcccg cagcgcgatg
     3181 aggcgcgcgc cgaggtcctg ggcggcatgc gccgcgcgct tgcgggcgct cttgctgggc
     3241 ttatcgtcga aagtctcgtc ggacatgact gctacaatgc ggccgatgca acagcgtgtg
     3301 gatggtacgc gaatcgccga actcaaaggc gtcatatcga gcgccctcga agcggcccgc
     3361 agggccggcg cgacccaggc cgaagccgat gccagcgtgc agcagggcct caccgtcaac
     3421 gtgcggttgg gcgcggtcga cacgatcgag taccagcgcg atcgcggcct cggcatcacc
     3481 gtctatgtcg gccagcgcaa aggttcggcg agcaccgccg acctggcgcc tgcggcggtg
     3541 gcggcgatgg tcgagaaggc cgtttcgata gcgcgcttca ccgccgagga tccgttcgca
     3601 gggctcgccg atccggatga actggcggtc gacataccgg atctcgacct cgaccatccg
     3661 tgggagctcg aaccgggcgc agcggtcgaa ctggcgcggc gctgcgaggc ggcgggcctg
     3721 gacctcgacg cgcgcatcac caattccgaa ggcgcgagca tctccaccca ccgggccgtg
     3781 cacgtctatg gcaactcgca cggtttcctc gccggctacc cgagttcgag ccacagcgtc
     3841 agctgtgcgc tggtcgcgca ggccggcagc gacatgcagc gcgactactg gtatacggcc
     3901 gcgcgcgatc ccgatgaact cggcgacatc gaggcgatcg gtcgtcgtgc cggcgagcgt
     3961 gcgctcggcc gcctcggcgc ccgtcgcctc ggcacccgcc gggcgccggt actgtttgcg
     4021 cccgacatcg cgcgcggcgt cttcgggcac atggtcggcg cgctgcgcgg cggaaaccag
     4081 taccgacgca cgtcctttct gctcgatgcg gccggcgagc ggatcctgcc gacgttcatc
     4141 cacatccacg agcgcccgca cctcgccagg gcgctcgcca gcagcccgtt cgatgccgag
     4201 ggtgtggcga cacgcgatcg cgaactcgtc accgccggtg tgctgcaggg ctacgtgctg
     4261 aacagctatg ccgcccgcaa gctcggcctg cgtaccaccg gcaacgccgg cggcgtgcac
     4321 aacctgatcg tcgagtcgga tcgcgatgcg ctgccgttcg aggcactgct cgcgcgcatg
     4381 ggcaggggtc tcgtcgtgac cgagctgatg ggccagggcg tcaacggcgt caccggcgac
     4441 tactcgcgcg gcgccaccgg gttctgggtc gaggggggcg cgcccgcctt cccggtcgag
     4501 gaagtcacga tcgccggcaa cctgcgcgac atgtatctcg cgatcgacgc ggtcggcagc
     4561 gacgtcgaca cgcgcggtgc gctgcgcacc gggtcgctgc tgatcggcga gatgacgatc
     4621 gccggcgaag cctaggcctc cgacccttcg gccaccagtt gcccgagcgt ccggtcctgg
     4681 cagcaggacc gccacgcctc gtcgatcgcg cgcgtcaggc ggtctaccgg cggcagtgaa
     4741 acatcgcgcg ccggtgcatg ccccatcttc tgattgcgtg ccgtgtcgag gatctcctgc
     4801 aggcggatgt ggccgatgtc gcggccgggc aacaggcgct cgtcgtcggt tgcgaggacc
     4861 agccccgcgc cctcgagcgt cgcgacgagc tgcgcgaccg cgatgcccgg cagtccgagt
     4921 tcactggcca gcgagctgac ggtccaggtg gtgtcgccgg tggcgtgcga gcggcccacg
     4981 agatacatga tcttgagcga gacgcgctcc acctcgatgc tcgacaggcg cagttcccgc
     5041 aggccgatgc gcaggtattc cgggttctgc acgtagaagg agacctgcgc cccgatcagc
     5101 aggatcagcc agccgaagta ggtccagagc agcgtcgcga tgatgatcgc gagacccgcg
     5161 tacacgatgg tgaggcgcga ggagtagacg acgaaggccg tgaacagcgt gcccacggcc
     5221 gcccacagca ccccgccgct gatgccgccg atcagcgccg actgccagcg caccctggtg
     5281 ttcggcacca gcatgtacac gaacgtgaag atcgcggtga ccatcacgta gggctcgagc
     5341 ccggtcgtga aatgcaccgc gcggctcagc agcggcagtt gcgtgatgac ctgccaggac
     5401 tcgctgcgca tggcgcggtg ggcgaggccg agaaaggcca ccagcagca
//
LOCUS       DAOQVY010000141         5356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_16884_length_5356_cov_4.864931, whole genome shotgun sequence.
ACCESSION   DAOQVY010000141 DAOQVY010000000
VERSION     DAOQVY010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16884_length_5356_cov_4.864931"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PKN91_11060"
     CDS             <1..380
                     /locus_tag="PKN91_11060"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNS28648.1"
                     /translation="RYGDVVEVGGNLRDARVCACVAAGIGRERIAIDPGFGFGKTLAH
                     NLALLRRLDVFVARGLPILVGLSRKRMIGELTGRAVDGRLAGSVALATIAALRGASIV
                     RVHDVAATVDALRIVAALGERTT"
     gene            377..1711
                     /gene="glmM"
                     /locus_tag="PKN91_11065"
     CDS             377..1711
                     /gene="glmM"
                     /locus_tag="PKN91_11065"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNS28649.1"
                     /translation="MSRTYFGTDGVRGRVGEEPMTVDFALRLASAAARVLAPRGGSVL
                     IGKDTRLSGYMFEAALEAGFVAAGVDVKLIGPLPTPGIAYMTRFFGCSFGVVISASHN
                     PYEDNGIKFFDGSGGKLSDELEREIEAEIANGTVTRGSRELGRAARVDKERIEYQKFC
                     ASTLPAGLDLEGLKIVLDCANGAAYKVGPRVFADLGAEVIPVGCSPNGRNINDGCGST
                     APQLLQLTVPGVRAHVGIALDGDGDRLVMIDHLGRVVDGDQLIFVIARARKEAGTLTG
                     PVVGTLMSNLGLEVALRGCGIEFRRAKVGDRYVLGMLKETGGIIGGETSGHILCLDRT
                     TTGDALVAALQVIGVMRQTGRSLAELAAGMSRFPQVLLNVKVAQRFDPSTVPAVNAAV
                     AKVEKDLGDEGRVVLRASGTEPVIRVMVEGREEAQTRAFAEEIATAVRAAAA"
     gene            1765..2208
                     /locus_tag="PKN91_11070"
     CDS             1765..2208
                     /locus_tag="PKN91_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HNS28650.1"
                     /translation="MRLTALTLLIAAASVAQAAEPAQTPALPRTPAPQDAVVYILSPA
                     DGATVTSPVTVRFGLKGMGIAPAGTKMEGTGHHHLLIDSELPANLALPLPATDKIVHY
                     GKGQTETTIELPPGKHTLQLVLGDQLHIPHDPPVISKQITITVKP"
     gene            2291..3061
                     /gene="tpiA"
                     /locus_tag="PKN91_11075"
     CDS             2291..3061
                     /gene="tpiA"
                     /locus_tag="PKN91_11075"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNS28651.1"
                     /translation="MSALSRRPLVAGNWKMHGSRAENAGLVEAVVSSHGLGAADCAVF
                     PPFVYLADVARLLRDSTVALGAQDVCAESQGAYTGEVSAAMLQDVGCVMVIVGHSERR
                     AIYAENDQLVARKFAAAQSRGLAPILCLGESLAEREGGQTEAVVARQLDAVLELVGAA
                     ALANAVIAYEPVWAIGTGRTATPGQAQDVHAFIRGRIAGRDAKIAGQVRILYGGSVKA
                     GNAAEIFAQPDVDGGLIGGASLKADEFLAIVAAAGQDR"
     gene            3079..3423
                     /gene="secG"
                     /locus_tag="PKN91_11080"
     CDS             3079..3423
                     /gene="secG"
                     /locus_tag="PKN91_11080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNS28652.1"
                     /translation="MLRTVLMFVQVFSSVAIIALVLIQRGKGADAGAGFGAGASGTVF
                     GARGARTGLSRATAAFAAVFMLNSLALAYLGTRNEAAPRTILDEIEASEVPVVPSPDA
                     APVPQAPAPAEQ"
     gene            3432..3516
                     /locus_tag="PKN91_11085"
     tRNA            3432..3516
                     /locus_tag="PKN91_11085"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3466..3468,aa:Leu,seq:gag)
     gene            3611..3979
                     /locus_tag="PKN91_11090"
     CDS             3611..3979
                     /locus_tag="PKN91_11090"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNS28653.1"
                     /translation="MLSNYLPILIYLGVAAGIGVALLVIGVLIGRYFTSLPDDAEKRS
                     AYECGFEAFEDSRRKFDVRYYLVAILFIVFDLEIAFLFPWAVALDAIGVPGLVAMGVF
                     LAILVVGFIYEWKKGALEWD"
     gene            3970..4467
                     /locus_tag="PKN91_11095"
     CDS             3970..4467
                     /locus_tag="PKN91_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005914280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNS28654.1"
                     /translation="MGLEADAADAAETYAQRGFLTTSIDSVISWARTGSMWPVTFGLA
                     CCAVEMMHAGASRYDLDRFGVIFRPSPRQSDVMIVAGTLVNKMAPALRKVYDQMAEPR
                     WVISMGSCANGGGYYHYSYSVVRGCDRIIPVDVYVPGCPPTAEALMYGILQLQKKIAR
                     STVAR"
     gene            4478..5140
                     /locus_tag="PKN91_11100"
     CDS             4478..5140
                     /locus_tag="PKN91_11100"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNS28655.1"
                     /translation="MSERIEELARNVDARLAGRVNRVPSLADELAYEVAAADLVDVCR
                     ALRDTDGLAFDTLIDVCGVDYLDYGRDEWRTASATRTGFSRGVNRNAARPAVEAGRFA
                     VVYHLLSVALNQRLRLRVYCEDSNDPMVPSVVGIWASANWFEREAFDLYGILFGGHPD
                     LRRILTDYGFIGHPFRKDFPLSGNVEVRYDPEKQRVVYEPVSIEPRVLVPRVIRRERG
                     NV"
     gene            5133..>5356
                     /locus_tag="PKN91_11105"
     CDS             5133..>5356
                     /locus_tag="PKN91_11105"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNS28656.1"
                     /translation="MSEIRNFTLNFGPQHPAAHGVLRLVLEMDGEVITKADPHVGLLH
                     RGTEKLAESKPFNQSIGYMDRLDYVSMMCN"
BASE COUNT          807 a         1780 c         1863 g          906 t
ORIGIN      
        1 cccgctacgg ggacgtggtc gaggtgggcg gcaacctccg cgacgcgcgc gtctgcgcct
       61 gcgtcgccgc gggcatcggc cgggaacgca tcgcgatcga tccgggattc ggcttcggca
      121 agacactggc gcacaatctt gcgttgctgc gccgtctcga cgtgttcgtg gcgcgcggtc
      181 tgccgattct ggtcggcctg tcgcgcaagc gcatgatcgg cgagctgacc gggcgcgcgg
      241 tcgacgggcg gctcgccggc agcgtggcgc tggcgacgat tgcggcgctg cgcggagcga
      301 gtatcgtgcg cgtccacgac gtcgcggcga cggtcgacgc gctgcgcatc gtggccgcac
      361 ttggggagag gacgacttga gcaggacata cttcgggacg gatggtgtgc gcgggcgggt
      421 cggcgaggag ccgatgaccg tcgatttcgc gctgcgactc gccagcgccg ccgcgcgtgt
      481 gctcgcgcca cgcggcggca gcgtgctgat cggcaaggac acgcgcctct ccggctacat
      541 gttcgaggcg gcgctcgagg cggggttcgt cgcggccggc gtcgacgtga agctgatcgg
      601 gccgctgccg acgcctggca tcgcctacat gacgcgcttt ttcggctgca gtttcggcgt
      661 cgtcatcagc gcctcgcaca acccgtacga ggacaacggc atcaaattct tcgacggaag
      721 cggcggcaag ctgtccgacg aactcgagcg cgaaatcgag gcggagatcg ccaacggcac
      781 ggtgacgcgc ggttcgcgcg aactcggccg cgcagctcgc gtcgacaagg agcgcatcga
      841 gtaccagaaa ttctgcgcgt cgactctgcc ggcgggcctc gatctcgagg gactcaagat
      901 cgtgctcgac tgcgccaacg gcgcggccta caaggtcggg ccgcgcgtgt tcgcggacct
      961 cggcgccgag gtgattccgg tcggctgctc gccgaacgga cgcaacatca acgatggctg
     1021 cggctcgacc gccccgcagc tgctgcagct caccgtgccg ggtgtgcgcg cgcatgtcgg
     1081 catcgcgctc gacggtgacg gcgatcgcct ggtgatgatc gaccacctcg gccgggtggt
     1141 cgacggcgac cagttgatct ttgtcatcgc gcgcgcgcgc aaggaagccg ggacgctcac
     1201 cggtccggtg gtgggcacgc tgatgagcaa cctgggcctc gaggttgcgc tgcgcggctg
     1261 cggcatcgag ttccggcgcg ccaaggtcgg cgaccgctat gtcctcggca tgctgaagga
     1321 gaccggcggc atcatcggcg gcgagacttc gggccatatc ctgtgtctcg atcggaccac
     1381 cacgggcgat gcgctggtgg cggcgctgca ggtcatcggc gtgatgcgcc agacggggcg
     1441 cagcctcgcc gaactcgccg cgggcatgtc gcgcttcccg caggtgctgc tgaacgtgaa
     1501 ggtcgcgcag cgcttcgatc cgtccaccgt gccggcggtc aatgcggcgg tggcgaaggt
     1561 cgagaaggac ctcggtgacg aggggcgcgt cgtgctgcgc gcgtccggga ccgaacctgt
     1621 catacgcgtg atggtcgaag gacgggaaga ggcgcagacc cgcgcctttg ccgaagagat
     1681 agccacggcc gttcgtgctg cggccgcctg agcgggctat cctgcgcgct cgattaccgg
     1741 gacgacccgg ggaaggaatt tgccatgcga ctgactgctc tcaccctgct gatcgctgct
     1801 gcctccgtgg cgcaggccgc cgagccggcg cagaccccgg cgctgccccg cacccctgcg
     1861 ccacaggatg cggtggtcta catcctgtcg cctgccgacg gcgcgaccgt cacgagcccg
     1921 gtcaccgtgc gcttcggcct caagggcatg ggcattgccc cggccgggac caagatggag
     1981 ggcaccggcc accaccacct gctgatcgat tccgaactgc cggccaatct cgccctgccg
     2041 ctgccggcga ccgacaagat cgtccactac ggcaagggcc agaccgaaac cacgatagag
     2101 ctgccgcccg gaaagcacac gctgcagctc gtgctcggcg accagttgca catcccgcac
     2161 gacccgccgg tcatctcgaa gcagatcacc atcaccgtga agccttgaag tccgcctggc
     2221 ggacgttgcg cggcgtggat ggcgccgtta tcatcgcgcg ccttttttac ggggcggccc
     2281 gcgtggccgc gtgagtgcct tgagtcgcag acctctcgtt gccggaaact ggaagatgca
     2341 cggctcgcgt gccgagaacg ccgggctcgt cgaagccgtg gtctcgagcc acgggctggg
     2401 cgcggccgac tgcgccgtct ttccgccgtt cgtctatctc gctgacgtcg cgcgcctgct
     2461 gcgcgactcg accgtggccc tgggcgcgca ggacgtgtgc gccgagtcgc agggggccta
     2521 cacgggcgaa gtgtccgcgg ccatgctgca ggacgtgggc tgcgtgatgg tcatcgtggg
     2581 ccactccgag cgccgcgcga tctacgccga gaacgaccag ctcgtcgcgc gcaagttcgc
     2641 agcggcgcag tcgcgcggcc tcgcgccgat cctgtgtctc ggcgaatcgc tcgccgagcg
     2701 cgaaggcggc cagaccgaag ccgtcgtggc gcgccagctc gacgccgtgc tggagctcgt
     2761 cggcgccgct gccctcgcca acgcggtgat cgcctacgaa cccgtctggg cgatcgggac
     2821 cggccgcacc gcgacgccgg ggcaggcgca ggacgtgcac gcattcattc gcgggcggat
     2881 cgccggccgc gatgctaaaa ttgcaggtca ggtccggatc ctttatggcg gcagcgtcaa
     2941 ggccggcaat gccgcagaga tcttcgcgca gcccgatgtg gacggcggtc tgatcggcgg
     3001 cgcatcactc aaggccgacg aattcctcgc gatcgtcgca gctgcgggtc aggacaggta
     3061 accggaaggt tcacgagcgt gcttcgtacc gtattgatgt ttgttcaggt gttcagctcg
     3121 gtggcaatca tcgcgctcgt gctgatccag cgtggcaagg gcgccgatgc cggcgcgggc
     3181 ttcggagccg gtgcgtcggg cacggtgttc ggcgcgcgcg gtgcgcgcac cggcctgtcg
     3241 cgggccaccg cggccttcgc cgccgtgttc atgttgaaca gcctcgcgct cgcctatctc
     3301 ggcacgcgca acgaggcggc gccgcgcacg atcctcgacg agatcgaggc gagcgaagtg
     3361 ccggtcgtgc cgtcgccgga tgcggcgccg gttccgcagg cgccggcgcc cgcggagcaa
     3421 taagtacttt tgccgacgtg gtggaattgg tagacacact agcttgaggg gctagcgccg
     3481 caaggtgtgg gagttcgaat ctccccgtcg gcaccacctt tgatacaatg cgcgccgcct
     3541 gaggggggca gaccgtcgtt cactgcgagc gaccgcctgt ccccttcgtt tcgtagcgaa
     3601 tcggcgggcc atgctcagca actatcttcc gatcctgatc tacctcggtg tcgcggccgg
     3661 catcggcgtc gcgctgctcg tgatcggcgt gctgatcggc cgctacttca cctcgctgcc
     3721 tgacgatgcg gaaaagcgct ccgcctacga gtgcggcttc gaggcgttcg aggacagccg
     3781 tcgcaagttc gacgtgcgct actacctggt cgcgatcctg ttcatcgtct tcgacctcga
     3841 gatcgcattc ctgtttccgt gggcggtcgc tctcgacgcc atcggcgtcc cggggctggt
     3901 cgcgatgggt gtgttcctcg cgatcctcgt cgtcggcttc atctacgaat ggaagaaggg
     3961 ggcgctcgaa tgggactaga ggccgacgcc gccgatgccg ccgagactta tgcgcagcgc
     4021 gggttcctga ccaccagcat cgacagcgtg atcagctggg cgcgcaccgg ttcgatgtgg
     4081 ccggtgacct tcgggctcgc ctgctgcgcg gtcgagatga tgcacgcggg cgcctcccgc
     4141 tatgacctcg accgcttcgg cgtgatcttt cgcccgagcc cgcgccagtc ggacgtgatg
     4201 atcgtcgccg gcaccctggt caacaagatg gcgccggcgc tgcgcaaggt ctacgaccag
     4261 atggccgagc cgcgctgggt gatctcgatg ggatcgtgcg ccaacggcgg cggctactat
     4321 cactattcct attcggtcgt gcgcggctgc gaccggatca tcccggtcga cgtttacgtg
     4381 cccggttgtc cgccgaccgc cgaggcgctg atgtacggca ttttgcagct gcagaagaag
     4441 atcgcgcgca gtacggtcgc acgctgaagg aaattcgttg tccgagcgta tcgaagagct
     4501 ggcccggaac gtggacgcgc gactcgcggg ccgggtgaac cgcgtgccgt cgctggccga
     4561 cgaactggct tacgaggtgg cggccgccga tctcgtggac gtgtgtcgcg cgctgcgcga
     4621 caccgacggc ctcgcgttcg acacgttgat cgacgtctgc ggcgtcgact acctcgatta
     4681 cgggcgcgac gagtggcgga ccgcatccgc cacccgcacg ggtttcagcc gcggcgtcaa
     4741 tcgcaatgcc gcgcgaccgg ccgtcgaggc ggggcgcttc gccgtcgtct accacctgct
     4801 gtcggtcgcg ctgaaccagc gcctgcgcct gcgcgtgtac tgcgaggaca gcaacgatcc
     4861 gatggtgccg agcgtggtgg gcatctgggc gtcggccaac tggttcgagc gtgaggcgtt
     4921 cgacctgtac ggcatcctgt tcggtggcca cccggacctg cgccgcatcc tcaccgacta
     4981 cggcttcatc ggccatccgt tccgcaagga tttcccgctg tccggcaacg tcgaggtgcg
     5041 ctacgacccc gagaagcagc gcgtcgtcta cgagccggtg tcgatcgagc cgcgcgtgct
     5101 cgtgccgcgg gtgatccgcc gggagcgcgg caatgtctga gatccgcaat ttcacgctga
     5161 acttcggtcc gcagcacccg gccgctcacg gcgtgctcag actcgtgctc gagatggacg
     5221 gcgaagtcat caccaaggcc gatccgcacg tcggcctgct gcaccggggc acggagaagc
     5281 tcgccgaatc gaagccgttc aaccagtcga tcggctacat ggaccggctc gactacgtgt
     5341 cgatgatgtg caacga
//
LOCUS       DAOQVY010000142         5344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_16938_length_5344_cov_5.758177, whole genome shotgun sequence.
ACCESSION   DAOQVY010000142 DAOQVY010000000
VERSION     DAOQVY010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16938_length_5344_cov_5.758177"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..557)
                     /locus_tag="PKN91_11110"
     CDS             complement(48..557)
                     /locus_tag="PKN91_11110"
                     /inference="COORDINATES: protein motif:HMM:NF021517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HNS28657.1"
                     /translation="MDRRKTMSTHAARIAIAPNCSLTPSGARSFFLGTCGVSFGIAGL
                     MALRGFWPVVAFAGLEMWLLGWALYASMQRRHRGETIEITEDSVAVERHLRGASVRTE
                     FPRHWSQVRLRRDVSPLRPTRLTIESGGRSCEIGSFLTEQERQHLAVRLAPLIGGMAE
                     SPPLPSGKP"
     gene            complement(598..1461)
                     /gene="bioC"
                     /locus_tag="PKN91_11115"
     CDS             complement(598..1461)
                     /gene="bioC"
                     /locus_tag="PKN91_11115"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: protein motif:HMM:TIGR02072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HNS28658.1"
                     /translation="MPPPETLDRAAVAREAGRAAASYDSVAALTRHIGAELLSRVEYF
                     DLAPRTVLDLGAGTGHASRALRARFRAALVIALDLSPGMLAIAARQARWPRRFERVAG
                     DACALPLRSGSIDLVHSSLALPSFADLDAALAEIRRVMTPRGLFLFSALGPDTLRELR
                     ESWAAADSAVHVHDFVDMHDLGSALTRAGFAEPVLDVEHYRRHFESLPALAAALRAAG
                     ARNAHAARPRGLTTRRRLATLTQAYEGRRENGRLPATFEVIYGAAFAGEPRAARAGGE
                     FVVSLDRLLSR"
     gene            complement(1448..2404)
                     /gene="bioB"
                     /locus_tag="PKN91_11120"
     CDS             complement(1448..2404)
                     /gene="bioB"
                     /locus_tag="PKN91_11120"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNS28659.1"
                     /translation="MNWTVAEVERLFAQPFVDLLFAAQQVHREHHDPHAVQLSTLLSI
                     KTGACPEDCAYCPQSIRYDTGVEREALLPLAEVRRRALAARAAGATRFCMGAAYRSPK
                     QKDLEHIVAMVREVRALGLESCATLGMLTPAQAAQLKDAGLDYYNHNIDTSAEFYGEI
                     ITTRDFQDRLDTLEAVRDAGIKVCCGGIVGMGESVRDRAAMLATLASLPEPPESVPIN
                     QLVPVPGTPLADAPPLDPFDFVRTIAVARIVLPRATLRLSAGRETMSDELQALCFLAG
                     AGSIFYGEKLLTTGNPDVERDRRLLERLGLARAGTLPRDAAA"
     gene            2809..3195
                     /locus_tag="PKN91_11125"
                     /pseudo
     CDS             2809..3195
                     /locus_tag="PKN91_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
     gene            complement(3217..4095)
                     /gene="ubiA"
                     /locus_tag="PKN91_11130"
     CDS             complement(3217..4095)
                     /gene="ubiA"
                     /locus_tag="PKN91_11130"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HNS28660.1"
                     /translation="MTLRSLSRTLTAYGQLMRLDRPIGTWLLLWPVLWALWIAGEGHP
                     PQQVFIIFVLGVVVMRAAGCVINDFADRDIDPQVKRTRERPLASRRISPYEAMALFGV
                     LICIAFWLVTRLDPFTIKLSVVGALLTVSYPLMKRWFPLPQFYLGLSFGGWGIPMAFA
                     ATLGTIPRVGWVLFFAAVLWAMVYDTLYAMVDRDDDLKIGVKSSAILFADLDRILIGA
                     MQLMMLFALHLAGRSMQFGAWYRAGLIAAAVLFLYQQWLVRNREPAACLRAFTNNNYV
                     GMAIFIGILLDYTFGH"
     gene            complement(4095..4640)
                     /locus_tag="PKN91_11135"
     CDS             complement(4095..4640)
                     /locus_tag="PKN91_11135"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: protein motif:HMM:NF016248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HNS28661.1"
                     /translation="MIETVPASTAPALWLPAGALGCYESDARMRSWLVTPGLLTERVR
                     AAAGAAFSLVVLGEDASHGEHRRTILLGTRAAPWIYAETSIPDASLATHPWLARMGEV
                     SLGEELTARGGTRSDFAYARLTPDAPLVARALARTALPPQTLWIRRSEFSIAGAPLTV
                     QEAFLPGIGVAGLDTHAARIA"
     gene            complement(4648..>5344)
                     /locus_tag="PKN91_11140"
     CDS             complement(4648..>5344)
                     /locus_tag="PKN91_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNS28662.1"
                     /translation="FPEERRAQIVERIAAACRQGRQVYWVCPLIEESDELRYQAAEET
                     AAQLAEALPDVRLGLVHGRMPAAKKEQAMAAFKAGRIQLLVATTVIEVGVDVPNATLM
                     VIENAERMGLAQLHQLRGRVGRGARESSCVLLYRRPLSQVARERLRTIRESNDGFEIA
                     RRDLELRGPGELLGTRQTGLAQLRVADLSRDADLLPRVQQAVEVILREHPDIIAPLTA
                     RWIGDGGRYGRVG"
BASE COUNT          827 a         1865 c         1887 g          765 t
ORIGIN      
        1 cgcggcgccg gtcttgtgca ttttgcgaat catttgaacc ttaagcctca cggtttgccg
       61 gacgggagcg gcggcgactc ggccatgcca ccgatcaacg gtgcaaggcg caccgcgagg
      121 tgctgtcgtt cctgctcagt caaaaaactg ccgatctcgc agctgcgccc gccggactct
      181 atcgtgagcc gtgtagggcg aagcggcgag acatctcgac gcagtctaac ctgtgaccag
      241 tgccgcggaa actcggtgcg caccgaagcg ccgcgcagat gccgttccac ggccacggaa
      301 tcctcggtga tctcgatcgt ttcgccccga tggcgacgct gcatgctggc gtacagcgcc
      361 cagccgagca gccacatttc gagacccgcg aatgcgacca ccggccagaa gccgcgcagc
      421 gccatcaacc ccgcgatgcc gaaggatacg ccgcaagtgc cgagaaagaa cgaccgcgca
      481 ccggacggcg tgagcgaaca gttcggggcg atggcgatgc gggcggcgtg cgttgacatc
      541 gtcttgcggc ggtccacggg ggcagcgagt ttagccccgg cattttcttc cggcaactca
      601 acggcttagc agccgatcga gagatacgac gaactcgccg ccggcgcgtg cggcccgtgg
      661 ctcgccggca aacgccgcgc catagatcac ctcgaaggtg gcgggcaggc ggccgttctc
      721 gcgccggccc tcgtaggcct gggtcagcgt cgcgaggcgg cggcgagtgg tgaggccgcg
      781 cggacgtgcg gcgtgcgcat tgcgtgctcc cgcggcgcgc agcgcggcgg cgagggcggg
      841 gagcgactcg aagtggcgcc tgtagtgctc gacatcgagc accggctcgg cgaagccggc
      901 acgcgtgagt gcgctgccga ggtcgtgcat gtcgacgaaa tcgtggacgt gcacggccga
      961 gtccgccgcc gcccagctct cgcgcagttc gcgcagcgta tcggggccga gcgcgctgaa
     1021 gaggaacaga ccccgcggcg tcatgacgcg ccggatttcc gcgagcgccg cgtcgaggtc
     1081 ggcgaacgag ggcagcgcga ggctcgaatg cacgagatcg atgctgccgc tgcgcagcgg
     1141 caatgcgcag gcatcgcccg ccacgcgctc gaagcggcgc ggccagcgcg cctggcgcgc
     1201 ggcgatcgcc agcatcccgg gtgacaggtc gagcgcgatg accagtgcgg ctcgaaagcg
     1261 cgcacgcagc gcgcggcttg cgtgccccgt gccggcgccg aggtcgagta cggtgcgcgg
     1321 cgcgaggtcg aagtactcga cccgcgacag cagctccgcg ccgatatggc gggtcagagc
     1381 ggctaccgag tcgtagctcg cggccgcgcg cccggcctcg cgggcgacgg ccgcacggtc
     1441 cagtgtttca ggcggcggca tcgcgcggca gtgtacccgc gcgcgcaagg ccgagccgct
     1501 cgagcaggcg ccggtcgcgc tcgacgtccg ggttgcccgt cgtgaggagc ttctcgccgt
     1561 agaagatcga gccggcaccg gcgaggaagc acagagcctg cagttcgtcg ctcatcgtct
     1621 cgcgacccgc cgacagccgc agggtcgcgc gcggcagcac gatgcgcgca accgcgatcg
     1681 tgcgtacgaa atcgaacggg tcgagcggcg gggcgtctgc gagcggcgtg ccgggtaccg
     1741 gcaccagctg attgatcggc acgctctcgg gcggctcggg caggctggcg agtgtcgcga
     1801 gcatggccgc gcggtcgcgc accgactcgc ccatgccgac gatgccgccg cagcacactt
     1861 tgatgcccgc atcgcgcacc gcttcgaggg tgtcgagccg gtcctggaag tcgcgcgtcg
     1921 tgatgatctc gccgtagaac tcggccgagg tgtcgatgtt gtggttgtag taatcgagac
     1981 ccgcgtcttt cagctgcgcg gcctgcgcgg gcgtcagcat gccgagcgtt gcgcaggact
     2041 cgaggccgag ggcgcgcacc tcgcgcacca tcgcgacgat gtgctcgagg tccttctgct
     2101 tcggtgagcg gtacgccgcg cccatgcaga agcgcgtggc gcctgccgcc ctcgcggcca
     2161 gcgcgcgccg gcgcacttcc gcaagcggca gcagtgcctc gcgctcgacg ccggtgtcgt
     2221 agcggatgct ctgcgggcag taggcgcaat cctcggggca ggcgccggtc ttgatcgaca
     2281 gcagtgtcga gagctgtacg gcgtgcgggt cgtggtgctc gcggtgcacc tgctgcgcgg
     2341 cgaacagcag gtcgacgaac ggctgggcga agagccgttc gacttcggca acggtccagt
     2401 tcatggcatt tctcgtcaaa gaggcaggaa atccgggcag acgcggcgac tccgcgccgc
     2461 aagactcgcg gcatgttccc gaggcctgct acgactgtca actttcgcga gtctctcaag
     2521 tcgacaattg cccgctacct gatccgcgct gaatgcctgt gcctgccgcc cacctgcgcg
     2581 ctgtgcggcg cccctggggg tttcgatccc gcgggccgcg ccttcgacct gtgtgcgcaa
     2641 tgcgaccgcg agctcccggc gaacgccgcg ccatccgggc tgggcggaca ggcgtgcgcg
     2701 gcgacctgga ccctgctcgt cgccccttgc cgctacgact acccgatcga ccactgcgtg
     2761 cgtgcgctca agttccacgg cgaggcggcc tgcgggcgcc tgctcggcat gctgatcgcg
     2821 cgtcatcggc gtgcgctcgg cgcaccctgg cccgatctcg tggttccggt gccgctgcac
     2881 cgggcgcgct atctcgagcg cgggttcaac caggcggcgg tcatcgcccg ccatgccgcg
     2941 cgtggcgtcg gactgccgct cgccaacggg ctgctcgagc gctgccgcgc gactgccgag
     3001 cagagccacc tgcgtcgcgc ggcacgcgcc ggcaatgtct cgggggcctt tcgcatgtcg
     3061 ccgcgcggcg agcgggtgct gccgggccgg cgcgtggcgc tggtcgacga cgtgttgacg
     3121 accggcagta ccgcgcgcga ggcgagccgc gtgctgcgcg ccgcgggcgc cattgccgtc
     3181 gagctgtggg tggcggcgcg cgccgcgccc gcgcactcag tgcccgaagg tgtaatcgag
     3241 caggatgccg atgaagatcg ccatgcccac gtagttgtta ttggtgaacg cgcgcaggca
     3301 ggccgccggc tcgcggttgc ggaccagcca ttgctggtag aggaacagga ccgccgcggc
     3361 gatgagcccc gcccggtacc acgccccgaa ctgcatgctg cgcccggcga ggtgtagcgc
     3421 gaacagcatc atcagctgca tcgcgccgat cagtatccgg tccaggtcgg cgaacaggat
     3481 cgcactcgac ttcacgccga tcttcagatc gtcgtcgcga tccaccatcg cgtagagcgt
     3541 gtcgtagacc atcgcccaca gtacggccgc aaagaacagc acccagccga cccgcgggat
     3601 cgtgccgagc gtcgcggcga aggccatcgg gatgccccag ccaccgaagg agagcccaag
     3661 gtagaactgc ggcagcggga accagcgctt catcagcgga tagctgaccg tcagcagcgc
     3721 gcccaccacc gatagcttga tcgtgaacgg atcgagccgg gtcaccagcc agaacgcgat
     3781 gcagatcagt acgccgaaaa gcgccatcgc ctcgtagggt gaaatgcgcc gcgaggcgag
     3841 cggccgctcg cgcgtgcgct tcacctgtgg gtcgatgtcg cggtcggcga aatcgttgat
     3901 cacgcaaccc gccgcgcgca tcaccacgac tccgagcacg aagatgatga agacctgttg
     3961 cggcgggtgg ccctcgccgg cgatccaaag cgcccacagc accggccaca gcagcagcca
     4021 ggtgccgatc gggcggtcga ggcgcatcag ctggccgtaa gctgtcagcg tgcgggacag
     4081 actgcgcagg gtcatcaggc gatccgtgcc gcgtgtgtat cgaggcccgc cacgccgatg
     4141 cccggcagga acgcttcctg cacggtgagc ggcgcgccgg cgatcgagaa ttccgaccgc
     4201 cgtatccaca acgtctgtgg cggcagcgcg gtgcgcgcca gcgcccgcgc gacgagcggc
     4261 gcatccggcg tcagccgcgc gtaggcgaaa tcggagcgcg tgccgccgcg cgcggtcagc
     4321 tcttcgccga gcgacacctc gcccatgcgc gcgagccacg gatgagtggc gagcgaggca
     4381 tccgggatcg aggtctccgc atagatccag ggcgccgcgc gagtgccgag caggatcgtg
     4441 cggcggtgtt cgccatgcga cgcatcctcg cccagcacca ccagactgaa cgccgcgccg
     4501 gcggccgcgc gcacgcgttc ggtgagcagc cccggtgtca cgagccacga gcgcatccgg
     4561 gcgtcgctct cgtagcagcc gagcgccccg gcgggcagcc agagtgcggg cgcagtcgat
     4621 gctggtacgg tttcgatcac tgttccccta gccgacacgg ccgtagcgcc cgccgtcgcc
     4681 gatccagcgc gcagtgagcg gcgcaatgat atcggggtgc tcgcgcagga tcacttccac
     4741 ggcctgctgt acgcgcggca gcaggtccgc atcgcgcgac aggtcggcga cccgcagctg
     4801 cgcgagcccg gtctggcgcg tgccgagcag ttcgcccggg ccgcgcaact cgaggtcgcg
     4861 acgcgcgatc tcgaaaccgt cgttcgactc gcggatcgtg cgcagccgct cgcgcgcgac
     4921 ctgcgacagc ggccggcggt acagcagcac gcagctgctc tcgcgggcgc cgcgcccgac
     4981 tcgtccacgc agctggtgca gctgcgcgag ccccatgcgc tcggcgttct cgatcaccat
     5041 cagcgtcgcg ttcggcacgt cgacacccac ctcgatgacc gtggtcgcga cgagcagctg
     5101 gatgcgcccg gccttgaacg ccgccatcgc ctgctccttc ttcgccgccg gcatgcgccc
     5161 gtgcacgagg ccgagccgta cgtcgggcag cgcctcggcg agttgcgccg ccgtctcctc
     5221 cgccgcctgg taacgcagct cgtcggattc ctcgatcagc gggcagaccc agtagacctg
     5281 ccggccctgg cggcaggccg cggcgatgcg ctcgacgatc tgcgcgcgcc gctcctcggg
     5341 gaag
//
LOCUS       DAOQVY010000143         5164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_17762_length_5164_cov_5.533568, whole genome shotgun sequence.
ACCESSION   DAOQVY010000143 DAOQVY010000000
VERSION     DAOQVY010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17762_length_5164_cov_5.533568"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PKN91_11145"
     CDS             complement(<1..192)
                     /locus_tag="PKN91_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28663.1"
                     /translation="MFRPGCPLLLSLLLAPAAALAQGDTRAEQQFANYAFAHELGSGL
                     YQAGEQLLQIYRLPLRWEYR"
     gene            230..2092
                     /locus_tag="PKN91_11150"
     CDS             230..2092
                     /locus_tag="PKN91_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS28664.1"
                     /translation="MSPSADKPSARRARDSLPPGPNDASLQGFFGVFLYSRRALSLVW
                     TTNRRLTIAIGVLTGLAGVLPAAVAWVGARIVDAVVTAVSSTHDPSVALRFVVVEGVL
                     VAAVAAAQRMLSLCQSLLRAQLGQRVNVMILEKALTLDLAHFEDSEFYDKLTRARREA
                     SSRPLSLVMRTFGLAQNAVSIVSFAVLLAQFSPWAVVVLIIAGLPAFVAETKFSGDAF
                     RLFRWRSPETRMQAYLETVLAREDYAKEVKLYGLGERFLERYRDIFRRLYKEDRALAI
                     RRDAWGLGLGLIATLTLYGAYAWIAIEAVRAVITLGEMTMYLMLFRQGQSAVSASLTA
                     IGGMYEDNLYLSTLYEYLETELPGEGGELVYGPHPEDGVRFEDVEFRYPDATEPAVSG
                     INLHLPPGASVALVGENGSGKTTLIKLLTRLYRPTRGRILLDGLDLEQWDGDALRGRI
                     GVIFQDFVRYQMQVGENIGAGDEEYFEDEERWRVAAGRGQADEFIEMLPAGYQTQLGK
                     WFRGGRDLSGGQWQKIALSRAFMRTRADILVLDEPTAAMDAQAEADVFEHFRKLAEGR
                     ITILISHRFSTVRMADQIVVLERGRIIERGSHAELVALGGRYAHLFALQAKGYR"
     gene            complement(2121..3806)
                     /locus_tag="PKN91_11155"
     CDS             complement(2121..3806)
                     /locus_tag="PKN91_11155"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNS28665.1"
                     /translation="MSNKSTSLLVLLMLCAWPVPHARAQTTALAAAIEARVSTAVLQG
                     ELSIRGQPIAATAAIADFYTRRGFAPAWTDPRNVDDLLRAIVDSADDGLSPADYHLAV
                     LAQMRPQMRLADTTDFGRADFELLLTDAILRLGYHLWFGKVDPEQFSAGWNMSRSLPG
                     LDAASELEAVLATGQLYRQIERARPAHPLYEALRGELAKMRRVALAGGWPELPEGPTL
                     EPGTSDARVPVLRELLVATGDLAAPPPPEPQAAPAGDPLLYDAMLVDAVRRFQKRMGL
                     NVDGRIGRGTQAELRVPVEDRIRQLRVNLDRGRVLLYQMPEEFLVVNIAGAEAAYLRG
                     DEVLWRNRAQVGTPYRETPEYRSELTYLVFNPTWTVPPTIIRNDILPAARRDPYSITR
                     RGLKVLDSSGAVIDPGSVDWSRFRTGHIPYTLRQDPGPDNALGRVKFMFPNPYNVYLH
                     DTPSKSLFDRDTRTTSSGCVRIEQPLELARLLLDDPVNWNDEAIAATIERGEIQNVTL
                     RRRVPIILAYWTAWVDPLGVVNFRRDVYGRDAAWAKALDAPFSFRRQPLVGGP"
     gene            3936..>5164
                     /locus_tag="PKN91_11160"
     CDS             3936..>5164
                     /locus_tag="PKN91_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNS28666.1"
                     /translation="MKHQLSVLLCAASLAAFAAQSSAAPAAAGSLPDATALALATPVI
                     GSRKIDPSLADVKGEIDVIVQLAGKPLAVANGQNSKSLGGLLNRQQQRVHSATLSRDQ
                     DAMLARLLALGGREVGRTRIAYNSVIVRIDAEQLYSVASYRDVVSVRPTREYSLDLTA
                     TVPYIGAAIAQAQGATGAGVTVGVIDSGIDYTHYNLGGEGTLAAYEAAYGSSIVDSRN
                     TTRDGLFPTSKVVEGHDFIGEQWPNAAAVQDDDPIDFQGHGTHVADIIGGLSADGLHK
                     GVAPDVKLIAAKVCSSVSTSCNGAAMLQALDYMLDPNGDGSMDDAVDVINMSIGSSYG
                     QIEDDTALASINAVNAGVVVVASAGNSADRPFITGSPGSTPEVLSVAQTEVPGALAYP
                     LVIDSPASIAGTYTNTAT"
BASE COUNT          813 a         1719 c         1788 g          844 t
ORIGIN      
        1 gcggtattcc cagcgcagcg gcaagcggta gatctgcagg agctgctcgc ccgcctggta
       61 gaggcccgag ccgagttcgt gggcgaacgc atagttcgca aattgctgct ctgcgcgtgt
      121 atcgccctgc gccagcgctg cggcgggtgc gagcaggagc gacagcagca acgggcagcc
      181 gggacgaaac acgccgccaa gattagccgt ttgcttcaga ctatgggcaa tgagcccgtc
      241 ggccgacaag ccatccgccc gccgcgcgcg ggacagcctg ccgcccgggc cgaacgacgc
      301 gtcattgcag ggtttcttcg gtgtgttcct gtacagccgc cgggcgctgt cgctcgtctg
      361 gacgaccaat cggcggctga cgatcgcgat cggggtgctc accgggctgg ccggggtgct
      421 gccggccgcg gtggcctggg tcggcgcgcg catcgtcgat gccgtggtga ccgcggtgtc
      481 gagcacccac gacccgtcgg tcgcgttgcg cttcgtcgtc gtcgagggcg tgctggtcgc
      541 cgcggtggcg gccgcgcagc gcatgctgtc gctgtgccag tcgttgctgc gcgcgcagct
      601 cggccagcgc gtcaacgtga tgatcctcga gaaggcgctg acgctcgatc tcgcccattt
      661 cgaggattcc gagttctacg acaagctgac gcgcgcccgt cgcgaggcat cgagccgccc
      721 gctgtcgctc gtcatgcgca ccttcgggct cgcgcagaac gcggtctcga tcgtcagctt
      781 cgccgtgctg ctcgcgcagt tctcgccatg ggcggtcgtc gtgctgatca tcgccggcct
      841 gcccgcgttc gtggccgaga ccaagttctc cggtgatgcg tttcgcctct ttcgctggcg
      901 ctcgcccgag acgcgcatgc aggcctacct cgagacggtg ctcgcgcgcg aggactacgc
      961 caaggaggtc aagctctacg gcctcggcga gcgtttcctc gagcgctatc gcgacatctt
     1021 ccggcgcctc tacaaggagg accgcgcgct cgcgatccgc cgcgacgcgt gggggctcgg
     1081 tctcggcttg atcgcgacgc tgacgctcta tggcgcctac gcctggatcg cgatcgaggc
     1141 ggtgcgcgcg gtcatcacgc tcggcgagat gaccatgtac ctgatgctgt tccgccaggg
     1201 ccagtccgcc gtatcggcga gcctcacggc gattggcggc atgtacgagg acaacctgta
     1261 cctgtcgacc ctgtacgagt acctcgagac cgagctgccg ggcgagggcg gcgagctggt
     1321 ctacggcccg caccccgagg acggcgtgcg tttcgaggac gtcgagttcc gctatccgga
     1381 tgcaaccgaa ccggcggtga gcggcatcaa cctgcatctg ccgcccgggg cgagcgtcgc
     1441 gctggtcggc gagaacggct cgggcaagac cacgctcatc aagctgctga cacgcctgta
     1501 tcggccgacc cgtggccgga tccttctcga cgggctcgat ctcgaacaat gggacggcga
     1561 tgcgctgcgc gggcgcatcg gggtcatctt tcaggacttc gtgcgctacc agatgcaggt
     1621 cggggagaac atcggcgcgg gcgacgagga gtatttcgag gacgaggaac gctggcgcgt
     1681 ggccgccggg cgcgggcagg ccgacgagtt catcgagatg cttcccgcag gctaccagac
     1741 ccagctcggc aagtggttcc gcggcggtcg ggacctctcg ggcgggcagt ggcagaagat
     1801 cgcgctgtca cgcgcgttca tgcgtacgcg cgccgatatc ctggtgctcg atgagccgac
     1861 tgccgccatg gacgcgcagg ccgaggccga cgtcttcgag cacttccgca agctcgccga
     1921 agggcggatc acgatcctca tctcgcaccg cttctccacc gtgcgcatgg ccgaccagat
     1981 cgtcgtgctc gagcgcgggc gcatcatcga gcgcggcagc cacgcggagc tggttgcgct
     2041 cggcggcagg tacgcgcacc tgttcgcgct gcaggcgaag gggtatcggt gaacggcgcc
     2101 gccggagtgc ggcgcgtggc tcagggcccg ccgacgagcg gctggcggcg gaagctgaac
     2161 ggggcatcca gcgccttcgc ccaggccgcg tcgcggccat agacgtcgcg ccggaaattc
     2221 accacgccca gcggatccac ccaggccgtc cagtaggcca ggatgatcgg gacgcgccgt
     2281 cgcaacgtga cgttctgtat ctcgccccgc tcgattgtcg cggcgatcgc ctcgtcgttc
     2341 cagttcaccg ggtcgtcgag cagcagcctg gcgagttcga gcggctgttc gatgcgcacg
     2401 cagcccgagc tggtggtgcg cgtgtccctg tcgaacagcg atttcgacgg cgtgtcgtgc
     2461 aggtagacgt tgtacgggtt cgggaacatg aatttgacgc gtccgagcgc gttgtccggc
     2521 cccggatcct gacgcagcgt atacgggatg tggccggtcc tgaaccgcga ccagtcgacg
     2581 ctgcctggat cgatcacggc gccgcttgaa tcgagcacct tgagcccgcg gcgcgtaatc
     2641 gagtagggat cgcggcgcgc ggcgggcagg atgtcattgc ggatgatggt gggcggcacg
     2701 gtccatgtgg gattgaacac gagataggtg agttcggagc ggtactccgg ggtttctcgg
     2761 tagggcgtgc cgacctgtgc gcgattgcgc cacaggacct cgtcgccgcg caggtaggcg
     2821 gcttcggcgc ccgcgatgtt gacgaccagg aattcctccg gcatctggta cagcagcacg
     2881 cggccgcggt cgagattgac ccggagctgg cgaatgcggt cttcgacggg cacgcgcagc
     2941 tcggcctggg tgcccctgcc gatgcggccg tcgacattga gtcccatgcg cttctggaag
     3001 cgcctgacgg catcgaccag catggcgtcg tacagcaacg gatcgccagc cggcgccgcc
     3061 tgcggctccg gcgggggagg cgctgcgagg tcgccggtcg cgacaagcag ttcgcgcagc
     3121 accgggaccc gcgcgtcact cgtgcccggc tcgagggtcg ggccctcggg cagttcgggc
     3181 cagccgcctg cgagcgcgac gcgccgcatt ttcgccagct cgccgcgcag tgcctcatac
     3241 agcggatgcg cgggccgcgc gcgttcgatc tgtcggtaga gctgcccggt ggcgagcact
     3301 gcctcgagtt ccgaggcggc atcgagcccg ggcaagctgc ggctcatgtt ccagcctgcg
     3361 ctgaactgct cgggatcgac tttgccgaac cacaggtggt agccgaggcg caggatcgcg
     3421 tcggtcagca gcagttcgaa gtcggcgcgc ccgaagtcgg tcgtgtccgc caggcgcatc
     3481 tggggccgca tctgcgcgag cacggcgagg tggtaatcgg cagggctcag gccgtcgtcg
     3541 gcgctgtcga cgatcgcgcg cagcaggtca tcgacgttgc gtggatcggt ccaggcgggc
     3601 gcgaaaccgc gccgcgtata gaagtcggcg atcgcggcgg ttgccgcgat gggttggccg
     3661 cggatcgaca gttcgccctg gaggaccgcg gtcgaaacgc gcgcctcgat cgccgccgcc
     3721 agggcagtgg tctgcgcccg cgcgtgcggg acaggccacg cgcagagcat caggaggacc
     3781 agcaacgacg tcgacttgtt tgacataaca gttcgcagaa aattcacact ctgtgatgca
     3841 accacttgac ttgcgctctg atatgctcgg cgcaagccgg catccgactg ggcggatgcc
     3901 tcagaaacca gaatcttcaa tccaggggga cattaatgaa gcatcaatta tccgtgctgc
     3961 tgtgcgctgc gagcctcgcg gcgttcgccg cacaaagttc cgcggctcca gcggccgcgg
     4021 gctcacttcc tgatgccacc gcgctcgccc tggcgacgcc ggtcatcgga tcgcgcaaga
     4081 tcgacccctc gctcgctgac gtcaagggcg agatcgacgt gatcgtgcag ctggccggaa
     4141 agccgctcgc ggtcgccaat ggccagaatt ccaagagcct gggcggcttg ctcaaccgcc
     4201 agcaacagcg cgtgcacagc gccacgctgt cgcgcgacca ggacgcgatg ctcgcccgcc
     4261 tgctggcgct cggcggccgg gaagtgggtc gcacgcgcat cgcctacaac tcggtgatcg
     4321 tgcgcatcga tgccgagcag ctctattcgg tcgcgagcta ccgcgacgtc gtgtcggtgc
     4381 ggccgacccg cgagtacagc ctcgacctga ccgccaccgt cccctacatc ggcgcggcga
     4441 tcgcgcaggc gcagggcgcc accggcgccg gcgtcaccgt cggcgtgatc gattcgggca
     4501 tcgactacac gcactacaac ctcggcggtg aaggcacgtt ggccgcctat gaagctgcgt
     4561 acggcagctc gatcgtcgat tcgcgcaaca ccacgcgcga cggactgttc ccgaccagca
     4621 aggtcgtcga gggccacgac ttcatcggcg agcagtggcc gaatgcagcg gcggtccagg
     4681 acgatgaccc gatcgacttc cagggccacg gcacgcacgt cgccgacatc atcggcggcc
     4741 tgtcggccga cggcctgcac aagggcgtgg cgcccgacgt caagctcatc gccgccaagg
     4801 tctgcagctc cgtttcgacg tcctgcaacg gcgcggcgat gttgcaggcg ctcgactaca
     4861 tgctcgatcc caacggcgac ggatcgatgg acgacgcggt cgacgtgatc aacatgtcga
     4921 tcggttcgag ctacggccag atcgaggacg acactgcgct cgcgagcatc aatgccgtga
     4981 atgccggcgt cgtggtcgtg gcctcggccg gcaacagcgc cgaccggccg ttcatcaccg
     5041 gctcgccggg ctcgactccc gaagtcctga gcgtggcgca gacagaagtg ccgggcgcgc
     5101 tggcgtaccc gctcgtcatc gattcgccgg cgagcattgc cggcacctac accaacacgg
     5161 ccac
//
LOCUS       DAOQVY010000144         5156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_17814_length_5156_cov_4.796903, whole genome shotgun sequence.
ACCESSION   DAOQVY010000144 DAOQVY010000000
VERSION     DAOQVY010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17814_length_5156_cov_4.796903"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PKN91_11165"
     CDS             complement(<1..282)
                     /locus_tag="PKN91_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28667.1"
                     /translation="MRTLCLALLVANVVFLGWALITPAAPPMHSSPAPDVPRLTLASE
                     AAAGPAPESGGDGAAGGVTSGQACRSIGPFGSEQEALRAAAAFREGGYEA"
     gene            complement(308..1060)
                     /locus_tag="PKN91_11170"
     CDS             complement(308..1060)
                     /locus_tag="PKN91_11170"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNS28668.1"
                     /translation="MTRLLLDVGNSRVKWAIARGPRLGRQRALALGARGAIPAAALLA
                     ALPGHLDSVRMVSVADTGATRRLASTLRRATGAPVHILRTTASAAGVRCGYREPWRLG
                     ADRWAAVIGAHHLFAPARAVCVVDVGTALTVDFVARDGRHRGGAIVPGPGLMIASLLR
                     DTQGIRRRAATSQAVGRTLYSRSTREAIEQGARYAAAAFIERALLEARRQFGGRPELV
                     VTGGGAPGVLPLVGGPCRLVPDLVLRGLAALG"
     gene            complement(1057..2058)
                     /locus_tag="PKN91_11175"
     CDS             complement(1057..2058)
                     /locus_tag="PKN91_11175"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNS28669.1"
                     /translation="MSAQQMPPLAQRVLRRLSDAGFASGQELAAELGVTRSAIWKAVH
                     VLEEAGVRIEAVSNRGYRLADGIAALDGVDISVRLPAAARARLARCEVAWSLPSTNAA
                     LLAGAEPQPGQAFVLLAEQQTAGRGRRGREWIAPLGGALCLSLAWTFAELPRDLAALS
                     LVVGTCALRALEARGARGLALKWPNDLVFADRKLGGILIEMRAESAGPVMVVIGIGLN
                     VALGSPTRERVTATGTDAIDLVEVGGSTDRNALAAALVAQLLDALPRFAKEGFAPFGA
                     AWREADALRGRPVAVSLGAERQVGIARGIDTAGALLVETPNGLRRFIAGEVTVRAQA"
     gene            complement(2058..2684)
                     /gene="plsY"
                     /locus_tag="PKN91_11180"
     CDS             complement(2058..2684)
                     /gene="plsY"
                     /locus_tag="PKN91_11180"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNS28670.1"
                     /translation="MSVVAIVAAYLLGSVSGALLVGRLRGGIDIRTLGSGNAGGTNAL
                     RTQGIGFALAVVVIDLGKGWIAAALLPLAAARFGAVPAGLPAACAFAAMVGHVWPVWH
                     GFRGGKGAATLVGALLALSPLALGVVLGVWLATVAILGFVGLASMLAAAALPLVVWFA
                     HIGPRGPMLAFGVAAALLVVVTHRANIARMRAGTEPRARRLWLFGRRP"
     gene            complement(2681..3613)
                     /locus_tag="PKN91_11185"
     CDS             complement(2681..3613)
                     /locus_tag="PKN91_11185"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HNS28671.1"
                     /translation="MTTQCTDTVLLVRPAAFGFNPDTAATNRMQREAALTPAEAQARA
                     CAEFNAMVGALRSEGIDVCVAEDTPEPAKPDAVFPNNWVSWHADGTVVLYPMCAPNRR
                     LERRAQILQRVAEATGFDIRRTLDLTAHERHGRYLEGTGSLVLDHVGRVAYACRSART
                     DERVLAEWCEAMGYSPELFTAVDRAGVPLYHTNVLMGIGRHFVAIGAGCIAEPDRARV
                     LARLAASGREIIELSLAEIEAFAGNLLELATWDEALGDSGVIVLSATARAQLAPERYA
                     RLAACSDVLLPIPVPTIEQLGGGSVRCMLAEVFR"
     gene            complement(3831..4526)
                     /gene="aqpZ"
                     /locus_tag="PKN91_11190"
     CDS             complement(3831..4526)
                     /gene="aqpZ"
                     /locus_tag="PKN91_11190"
                     /inference="COORDINATES: protein motif:HMM:NF003838.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HNS28672.1"
                     /translation="MPLLNRVAAEFLGTLWLVLGGCGSAVLAAKFPEVGIGLVGVALA
                     FGLTLLTMVYAIGHISGCHLNPAVTIGLFVGGRCKAGDVVPYVVAQVLGGIAAAWILL
                     TIASGNGTDVLAGGLASNGYGEHSPGGYALGAAFLCEVVMTFFFLLIIMGATDKLSSP
                     GFAGIAIGLALTLIHLISIPVTNTSVNPARSTGPAVFVGGWAISQLWLFWVAPMIGAA
                     LGGVAYRWLGRGK"
     gene            4613..>5156
                     /locus_tag="PKN91_11195"
     CDS             4613..>5156
                     /locus_tag="PKN91_11195"
                     /inference="COORDINATES: protein motif:HMM:NF025587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HNS28673.1"
                     /translation="MTEHVPTLSAGAIETDAFARAIATAYERYGFVVIADHGIDQPLI
                     DGCLDACRAFFALPDEVKRRYHLPGTGGARGYTPFGVETAKGADHHDLKEFWHVGREL
                     PPGHRHAASMPPNLWVAEVPRFRERTLALWEAFDALGRRLLTAIAHALGLPRDYFEDK
                     VDLGNSVLRVLHYPPTADTTA"
BASE COUNT          834 a         1895 c         1777 g          650 t
ORIGIN      
        1 cgcctcgtag ccaccctcgc gaaaggcggc ggcggcccgc agcgcttcct gctcgctgcc
       61 gaagggaccg atcgagcgac aggcctggcc gctcgtcacg ccacctgcgg cgccatcgcc
      121 gccgctctcc ggggcggggc ccgcggcggc ttcgctcgcg agggtgaggc gcggaacgtc
      181 cggtgccggg gaactgtgca tcggcggggc ggcgggcgtg atcaacgccc agccgaggaa
      241 gacgacattg gcaacgagca gggcgaggca cagcgtacgc acggcgcgag tctactccag
      301 gcgcgcctca gccgagcgcg gcgaggcctc gcagtacgag atccggcacc agccggcagg
      361 gaccgccgac cagcggcaga acgcccggcg cgccgccacc cgtcaccacg agttcggggc
      421 ggccgccgaa ctggcgccgc gcctcgagca gcgcacgctc gatgaacgcc gccgccgcgt
      481 agcgggcgcc ctgctcgatc gcctctcgag tgctgcgcga atacagcgtg cggccgactg
      541 cctgcgacgt tgcggcgcgg cggcggatgc cctgcgtatc gcgcagcagg ctcgcgatca
      601 tcaatccggg tcccgggacg atggcgccgc cgcgatggcg accgtcgcgc gcgacgaagt
      661 ccacggtcag tgccgtgccg acgtcgacga cacagaccgc ccgcgccggc gcgaagaggt
      721 gatgggcccc gatcacggcc gcccagcgat ccgcgccgag gcgccacggc tcgcggtaac
      781 cgcaacgcac gccggcggcg ctcgcggtgg tgcggaggat gtgcacgggc gcgccggtcg
      841 cgcggcgcaa cgtgctcgcc agccggcgtg tcgcgcccgt atcggccacc gagaccatcc
      901 gcacgctgtc gagatggccc ggcagcgcgg cgagcagcgc cgcggcggga atggcgccgc
      961 gagcgccgag cgcgagcgcc cgctgcctgc cgagtcgcgg gccgcgcgcg atggcccact
     1021 tcacgcggct attgccgaca tcgagcaaca ggcgggtcac gcctgcgccc tcaccgtcac
     1081 ttcgccggcg atgaagcgcc tgagcccatt cggcgtctcg acgagcagtg ctccagccgt
     1141 atcgatgccg cgcgcaatgc cgacctggcg ctccgcgccc aggctcaccg cgaccgggcg
     1201 gccgcgcagc gcgtccgctt cgcgccaggc cgcgccgaac ggcgcgaagc cctccttcgc
     1261 gaaccgcggc agggcgtcga gcagctgcgc gaccagcgcc gcagcgagcg cgttgcgatc
     1321 cgtgctgcca ccgacctcga cgagatcgat ggcgtcggtg ccggtcgccg tcacgcgctc
     1381 acgggtgggc gagccgagtg ccacgttcag tccgatgccg atcacgacca tcacaggccc
     1441 ggccgactcc gcccgcatct cgatcagtat gccgccgagc ttgcggtcgg cgaaaacgag
     1501 atcgttcggc cacttgagcg cgagtccgcg cgcaccccgc gcctcgagcg cgcgcagtgc
     1561 gcaggtgccg acgaccagcg agagcgcggc aaggtcacgc ggcagttcgg caaaagtcca
     1621 ggcgagcgaa agacacagcg caccgcccaa cggggcgatc cactcgcgcc cgcgccggcc
     1681 acggcccgcc gtctgctgct cggccagcag cacgaaggcc tggcccggct ggggctccgc
     1741 accggcgagc aaggcggcgt tcgtcgacgg caggctccag gcgacttcgc agcgcgcaag
     1801 gcgcgcgcgc gccgcggccg gcagacgcac gctgatatcg acgccgtcga gcgcggcaat
     1861 gccgtcggcc agccgatagc cgcgattcga caccgcctcg atgcgtacgc cggcttcctc
     1921 cagcacatgc acggctttcc agatcgcgct gcgggtaacg ccgagttcgg cagccagttc
     1981 ctgcccggac gcgaatccgg catcgctcag ccggcgcagg acacgctgcg cgagcggcgg
     2041 catctgctgc gccgacatca cgggcgccgc ccgaacagcc acaggcggcg cgcccgcggc
     2101 tcggtgccgg cgcgcatgcg cgctatgttg gcgcgatggg tgacgaccac gagcaatgcc
     2161 gccgccacgc cgaaggccag catcgggccc cgcgggccga tgtgcgcaaa ccacaccacc
     2221 agcggcaacg cggctgcggc cagcatcgag gcgagtccga cgaagcccag aatcgccacc
     2281 gtggcgagcc acacgccgag caccacgccg agcgccagcg gcgacagcgc gagcagcgcg
     2341 ccgaccagcg tcgcggcgcc cttgccgccc cggaatccgt gccacaccgg ccaaacgtga
     2401 ccgaccattg ccgcgaatgc gcaagcggcc ggcaggccgg ccggcacggc gccgaagcgc
     2461 gccgccgcga gcggcagcag cgccgcggcg atccagccct tgccgaggtc gatgacgacg
     2521 acggccagcg cgaagccgat gccctgcgta cgcagtgcat tggtgccgcc cgcattgccg
     2581 ctgccgagcg tgcggatgtc gatgccacca cgcaatctgc cgacgagcag tgcgccgctc
     2641 accgacccga gcaggtaggc ggcgacgatc gcgacgacgc tcaccggaac acctccgcca
     2701 gcatgcagcg cacgctgccg ccgcccagct gctcgatcgt cggcaccggt atcggcagca
     2761 gcacatcgct gcaggcggcg aggcgtgcgt agcgctcggg tgcgagctgc gcacgcgccg
     2821 tggccgacag cacgatgacg ccactgtcgc cgagcgcctc gtcccaggtg gccagttcga
     2881 gcagattgcc ggcaaaggct tcgatctcgg cgagactcag ctcgatgatc tcccggccgc
     2941 tggccgcgag ccgcgcgagt acgcgtgccc gatcgggctc ggcgatacac ccggcgccga
     3001 tcgcgacgaa atgccggccg atgcccatga gcacgttcgt gtggtagagc ggcacgccgg
     3061 cgcgatcgac cgccgtgaac aactcgggcg agtagcccat cgcctcgcac cactccgcca
     3121 gcacgcgctc gtccgtgcgc gccgagcgac acgcgtaagc gacgcgcccg acatggtcaa
     3181 gtaccaggct gccggtgccc tcgaggtagc ggccgtggcg ttcgtgcgcc gtgagatcga
     3241 gcgtacgccg gatgtcgaag ccagtggcct cggcgacccg ctgcaggatc tgcgcgcggc
     3301 gctcgaggcg gcggttcggt gcgcacatcg ggtagagcac cacggtaccg tccgcgtgcc
     3361 agctcaccca gttgttcgga aagacggcgt cgggcttggc cggctcgggc gtatcttccg
     3421 cgacgcagac gtcgataccc tcgctgcgca gcgcgccaac catcgcattg aactcggcgc
     3481 aggcgcgcgc ctgcgcctcg gccggcgtga gcgccgcctc gcgctgcatg cgattggttg
     3541 ccgccgtatc gggattgaag ccgaacgcgg ccggcctcac caggaggacc gtgtcggtgc
     3601 actgggtcgt catcggaatg tcggcaggct ttacagttcc cggtcggggg atgatcgtga
     3661 cgcggcaaag atagtttatc ttcagagagt tagcacgcgt ggcacgcgcg ttgctgccta
     3721 cctgccagct cttcgaaaga gagcgtccgc atggggggtc gagcgcgacc gtcaggttgc
     3781 gcccggggaa tgccccgcga tcgaaatcgc ggggcgttct ttttggcgcc ctacttgccg
     3841 cgtccgagcc agcggtacgc gacgccgccg agcgcggccc cgatcatcgg tgccacccag
     3901 aacagccaca gctgcgagat cgcccagccg ccgacgaaca cggcgggtcc cgtgctgcgg
     3961 gccgggttca ccgatgtgtt ggtcaccgga atgctgatca ggtgtatcaa ggtgagcgca
     4021 aggccgatcg cgatgccggc gaagcccggc gaggacagct tgtcggtcgc gcccatgatg
     4081 atcagcagga agaaaaaggt catcacgacc tcgcacagga aggctgcgcc gagcgcgtag
     4141 ccgcccgggg agtgctcgcc gtagccattc gacgcgagcc cgcccgcgag cacatcggtg
     4201 ccgttgccgc tcgcgatcgt cagcaggatc cacgccgcgg cgatgccgcc caggacctgc
     4261 gcgacgacgt agggtacgac gtcaccagcc ttgcagcggc cgccgacgaa gagaccgatc
     4321 gtcaccgccg gattgaggtg acagccggag atgtggccga tcgcatagac catcgtcaac
     4381 agggtcaggc cgaatgcgag cgcgaccccg accagcccga taccgacctc cgggaacttc
     4441 gcggccagca cggcgctgcc acagccaccg agcacgagcc acagggtgcc gaggaattcc
     4501 gcggcaacac gattgagcaa cggcattgcg aacccccttt ttgttgtcag tccggccgga
     4561 ttatggcccg ccatggcgcc cctgtcgcgc gcagcgcaga atggcgccac acatgaccga
     4621 acatgtcccc actctctccg ccggggcgat cgagacggac gcattcgctc gcgccatcgc
     4681 caccgcctac gaacgctacg gcttcgtggt gatcgccgac cacgggatcg accagccgct
     4741 catcgacggc tgcctcgacg cctgccgcgc cttcttcgcg ctgcccgatg aggtgaagcg
     4801 ccgctaccac ctgcccggca ccggcggcgc gcgcggttac acgcccttcg gcgtcgagac
     4861 cgcaaagggc gccgaccatc acgacctgaa ggaattctgg cacgtcgggc gcgagctgcc
     4921 gccgggtcat cgtcacgccg cctcgatgcc gccgaacctg tgggtcgcgg aagtgccgcg
     4981 ctttcgcgag cgcacactcg cgctctggga ggccttcgat gcgctcggcc gtcggctgct
     5041 gaccgccatc gcgcacgcgc tcggcctgcc gcgcgattat ttcgaggaca aggtcgatct
     5101 cggcaacagc gtgctgcgcg tgctgcacta tccgccgacc gccgatacga cggcgg
//
LOCUS       DAOQVY010000145         5151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_17835_length_5151_cov_5.819270, whole genome shotgun sequence.
ACCESSION   DAOQVY010000145 DAOQVY010000000
VERSION     DAOQVY010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17835_length_5151_cov_5.819270"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PKN91_11200"
     CDS             <1..210
                     /locus_tag="PKN91_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28674.1"
                     /translation="AHAQALHPERDGRSAERVLAAVDRLLAGAGARRAKPLNLLRKWR
                     IRRRLAHWERTGEGRTKAAPLRGRR"
     gene            207..1883
                     /locus_tag="PKN91_11205"
     CDS             207..1883
                     /locus_tag="PKN91_11205"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS28675.1"
                     /translation="MNASFERWRANTLGPFQVRIFAAIWIATLISTFGSLIQGVGASW
                     LMLSIAPSADMVALVQTSTTLPIMLLSLVAGSISDIWDRRRLMLVALGIMVAVSVALA
                     IVSWLGHITPWMLLGFTFILGCGAALYGPAWQSSVGEQVPRELVPDAVALNSLGFNIA
                     RTVGPAIGGAIVAAFGATFAFAINAVSYVGLIVVLASWKRPAPRRTLPPESLGVAMIA
                     GLRYVRLSPALVAIFVRALVFGFLGSAVWALLPLVARDLVAGGAITYGVLLGAFGGGA
                     VVGALTVAQLRQRMSNETLVRVASVMFGLGTIAVAMSPWLATTILALVAAGAAWVITL
                     STLNTSVQVSSPRWVVGRSVAIYQMVTFGGLAIGSWAWGEIAHTSGLRYSLIGAGCGM
                     LASIALGRWLRTPQAESLDLESLRVDIPATPPKVDIVPQSGPVVINVEYRVAAEDRLA
                     FEAAMIELQRVRRRDGARGWTLLRNMDDPEVWIERFHSPTWVAHLRRRLRLTKADRDI
                     EERVRRYHRGDGAPRVIRYLERPPTAMATLAKSEAERIGERTARTDPTIS"
     gene            1896..2555
                     /locus_tag="PKN91_11210"
     CDS             1896..2555
                     /locus_tag="PKN91_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28676.1"
                     /translation="MLKFVFALLPALACAGCLQGDGNERINGPVNVAAGQPAADASTV
                     NGSIKVAPGAAVLAAGTVNGSISLGEGASASQLGTVNGSIALGEQARVAGDVSTVNGD
                     IKLARGADVTGSLRNVNGLFTIDAAHVGGDIKTVAGNIHVGTDSRIDGGIRIEHSTGM
                     TVTIGEKIPVIVIAPGAVVAGPLKFDREVKLYVSDRATIGEVIGATPVMFSGDQPPTE
                     T"
     gene            complement(2558..2983)
                     /gene="arfB"
                     /locus_tag="PKN91_11215"
     CDS             complement(2558..2983)
                     /gene="arfB"
                     /locus_tag="PKN91_11215"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNS28677.1"
                     /translation="MTIQISRQLEIPDSDLSVTFIRASGPGGQNVNKVASAVQLRFDL
                     AGTTVLAPRVKERLRALAGQRLTDDGAILIQARSHRSQAGNRRDAEERLADLVRRALI
                     EPKQRRPTRPTRASKERRLESKARAQRTKRLRARVGRDD"
     gene            complement(2946..4301)
                     /locus_tag="PKN91_11220"
     CDS             complement(2946..4301)
                     /locus_tag="PKN91_11220"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNS28678.1"
                     /translation="MNKNDGAVQAFFATLTDPTILLELFAILISALVAIALAGFLRAW
                     YRRGYGSAHRSGFSARVLDATVIVLPILLTLGVLFAVYVAFAAKGLGTGLLNRAMQLT
                     AALLVVRFGVYLLRQLLGEDSWLKGWQNQFTFVVWLAVAFELLGWFERVEGAMNNIDL
                     LPGKETFTVWELLKTLVIISIIVMATSLIARTIERRVMKMTALAMSTRLGISKFSYFF
                     LITLGLLLGFNAAGVDFTALTLLTGAIGVGLGFGLQAIASNFLSGFVLLMDKSIKPGD
                     VISFTSAAGPTSGGFTADNFGWVQELRGRYIVVRDRDGIETLVPNQFVITNSVINWSY
                     SDARVRLRLPVMISYRDDPEKALLLLQHAAADHPRILREPGPVTRLMSFEDYGMRLEV
                     RFWIADPMNGVNNVRSDVNRAIVRLFREAGITIPVAQRELRMLPALDNDHPDLAPARD
                     T"
     gene            complement(4329..4553)
                     /locus_tag="PKN91_11225"
     CDS             complement(4329..4553)
                     /locus_tag="PKN91_11225"
                     /inference="COORDINATES: protein motif:HMM:NF016231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HNS28679.1"
                     /translation="MIEIEDNVDRELFRNIEKLRQLRIEHRDLDQVIERLSMDIHVDE
                     LQLKRLKRRKLGLKDQITRLESELIPDLNA"
     gene            complement(4553..>5151)
                     /locus_tag="PKN91_11230"
     CDS             complement(4553..>5151)
                     /locus_tag="PKN91_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28680.1"
                     /translation="DAVATGIAAQIDDVTRRRRERVLAIGERLATRSRLPGRDIAART
                     LEGGGVPTRMHLARSLVALGYADDTQHAFDRWLNRDRPGHVPVEWPELDTALPRLVES
                     CRATVLAHPHRYRLSGGALRQLVTDFTAGGGSALEVSMAGMSPGDHDRLASLARRHAL
                     AGSAGSDFHDPAVPWNPLGRFAKLPDGIAPVTERIADS"
BASE COUNT          758 a         1683 c         1860 g          850 t
ORIGIN      
        1 gcgcatgcgc aggccctgca tcccgagcgt gacggccgct ccgccgagcg cgtgctcgcg
       61 gcggtcgaca ggctgctcgc gggcgccggc gcgcggcgcg cgaagccgct caacctgctg
      121 cgcaagtggc gcatccgtcg ccggctcgcg cactgggaac gcaccggcga ggggcgcacc
      181 aaagccgccc cgctgcgtgg tcgccgatga acgccagctt cgagcgctgg cgcgcgaaca
      241 ccctcgggcc ctttcaggtg cgcattttcg cggccatctg gatcgcgacg ctcatctcga
      301 ccttcggcag cctgatccag ggcgtcggcg cctcctggct gatgctgtcc atcgcgccgt
      361 cggccgacat ggtggcgctg gtgcagacgt cgaccacgct gccgatcatg ctgctgtcgc
      421 tggtcgcggg gtcgatctcg gacatctggg accgccgtcg cctgatgctg gtcgcgctgg
      481 gcatcatggt cgcggtgtcg gtcgcactcg cgatcgtatc ctggcttggt catatcacgc
      541 cgtggatgct gctcggcttc acgttcatcc tcggctgcgg cgcggcgctc tacgggccgg
      601 cctggcagtc gtcggtcggt gagcaggtgc cgcgcgagct ggtgccggat gccgtggcgc
      661 tcaacagcct cggcttcaat atcgcgcgca ccgtcggacc ggcgatcggc ggcgcgatcg
      721 tcgcggcgtt cggcgccacg ttcgcgttcg cgatcaatgc ggtctcgtat gtcgggctca
      781 tcgtcgtgct cgcctcatgg aagcggccgg cgccacggcg tacgctgccg cccgagagcc
      841 tcggcgtcgc gatgatcgcg ggcctgcgct atgtgcgcct gtcgcccgcg ctggtggcca
      901 tcttcgtccg cgccctggtg ttcggcttcc tgggcagcgc ggtgtgggcc ttgctgccgc
      961 tcgtcgcgcg tgacctggtc gccggcggcg cgatcaccta cggcgtgctg ctcggcgcgt
     1021 tcggcggtgg tgcggtcgtc ggtgcgctga cggtcgccca gctgcgccag cgcatgtcga
     1081 acgagaccct ggtgcgcgtt gccagcgtga tgttcgggct gggcacgatt gcggtcgcga
     1141 tgagtccctg gctcgccacg acgattctgg cgctggtcgc ggccggcgcc gcctgggtga
     1201 tcactctctc gacgctcaat acctcggtgc aggtgtcctc gccgcgatgg gtcgtcgggc
     1261 gctcggtggc gatctaccag atggtgacct ttggtggcct cgcgatcggc agctgggcgt
     1321 ggggcgagat cgcgcatacc tcgggcctgc gctacagcct gatcggcgcg ggctgcggca
     1381 tgcttgcctc gatcgcgctc gggcgctggt tgcgtacgcc ccaggccgag agcctcgacc
     1441 tcgaatcgct gcgtgtcgac ataccggcga cgccgcccaa ggtcgatatc gtgccgcaga
     1501 gcgggccggt cgtgatcaat gtcgagtacc gggtcgcggc ggaggaccgg ctcgcgttcg
     1561 aggcagcgat gatagagctg cagcgcgtgc ggcgccgcga cggggcccgc ggctggacgc
     1621 tgctgcggaa catggacgac ccggaggtgt ggatcgaacg cttccacagt cccacctggg
     1681 tggcgcacct gcgccggcgc ctgcgcctga cgaaggcgga tcgcgacatc gaagagcgcg
     1741 ttcgccgtta tcatcgcggt gacggcgcgc cgcgggtgat ccgctatctc gagcgcccgc
     1801 cgactgccat ggccacgctg gccaagtcgg aggccgagcg catcggtgag cgtaccgcca
     1861 ggaccgatcc gacgatttcg taaaggagca tcgaaatgct gaagttcgta tttgcactgc
     1921 tgccggccct tgcctgtgcg ggctgtctcc agggcgatgg caatgagcgc atcaacgggc
     1981 cggtcaacgt ggctgcaggg cagcccgccg cggacgcatc gaccgtgaac ggttcgatca
     2041 aggtggcgcc cggcgcggcg gtgctcgccg ccgggacggt caatggctcg atttcactcg
     2101 gcgagggcgc gagcgccagc cagctgggca cggtcaatgg ttcgatcgcg ctcggcgagc
     2161 aggcgcgcgt ggccggcgac gtgtcgacgg tcaatggcga catcaagctg gcccggggcg
     2221 cggatgtcac cggctcgctc cgcaatgtca acggtctgtt cacgatcgac gccgcgcacg
     2281 tcggcggcga catcaagacc gtggccggca acatccatgt cggcaccgat tcgcgcatcg
     2341 acggcggcat ccgtatcgag cactcgaccg gcatgaccgt cacgataggc gaaaagattc
     2401 cggtcatcgt catagcgccc ggcgcagtgg tcgcgggtcc gctcaagttc gaccgcgaag
     2461 tgaagctcta tgtgagcgac cgcgcgacga tcggcgaggt catcggagcc acgccggtga
     2521 tgttcagcgg cgatcagccg ccgaccgaga cctgaggcta gtcgtcgcgc ccgacgcggg
     2581 cgcgcaggcg cttggtgcgc tgggcgcgcg ccttggattc gagccggcgt tccttcgagg
     2641 cgcgggtcgg tctggtgggg cggcgctgct tcggttcgat cagcgcgcgg cgcacgaggt
     2701 ccgcgagccg ctcctcggcg tcgcggcggt tgccggcctg gctgcgatgg gacctggcct
     2761 ggatcaggat cgcgccgtcg tccgtcaggc gctggccggc gagcgcgcgc agcctctcct
     2821 tgacgcgcgg cgcgagcacc gtggtgccgg cgaggtcgaa gcgcagctgc acggccgagg
     2881 cgaccttgtt gacgttctgc ccgccgggac ccgacgcgcg gatgaacgtc accgacaggt
     2941 cgctgtcagg tatctcgagc tggcgcgaga tctggatggt cattgtcgag tgcgggcaac
     3001 atgcgcagct cgcgctgggc caccggaatc gtgatgcccg cctcgcggaa gaggcggacg
     3061 atcgcgcggt tcacgtcgga acgcacgttg ttcacgccgt tcatcggatc ggcgatccag
     3121 aagcgcacct cgaggcgcat gccgtagtcc tcgaacgaca tcaggcgcgt gaccggcccg
     3181 ggctcgcgca ggatgcgcgg atgatcggcg gccgcgtgtt gcagcagcag cagcgccttc
     3241 tcggggtcgt cccggtagct gatcatgacc ggcaggcgca ggcgcacgcg ggcgtccgag
     3301 tagctccagt tgatcaccga attggtgatc acgaactggt tgggcaccag ggtctcgatg
     3361 ccatcgcggt cgcgcacgac gatgtagcgg ccgcgcagtt cctgtaccca gccgaagttg
     3421 tcggcggtga agccgccgga ggtgggtccc gcggcgctgg tgaagctgat cacgtcgccg
     3481 ggcttgatcg atttgtccat cagcagcacg aagccggaga ggaaattgct ggcgatcgcc
     3541 tgcaagccga agccgaggcc gacgccgatc gcgccggtga gcagcgtgag cgcggtgaag
     3601 tcgacgcccg ccgcattgaa gccgagcagc aggccgaggg tgatcaggaa gaagtaggaa
     3661 aatttcgaga tgccgaggcg cgtcgacatc gcgagtgcgg tcatcttcat cacgcgccgc
     3721 tcgatggtgc gcgcgatcag cgacgtggcc atcacgatga tcgagatgat caccagggtc
     3781 ttcagcagct cccagaccgt gaaggtttcc ttgccgggca gcaggtcgat gttgttcatc
     3841 gccccttcga cgcgctcgaa ccagccgagc agctcgaagg cgaccgcaag ccagacgacg
     3901 aacgtgaact ggttctgcca gcccttgagc caggagtcct cgccgagcag ctggcgcagc
     3961 aggtagacgc cgaagcgcac caccagcagc gcggcggtga gctgcatggc gcgattcagc
     4021 aggccggtcc cgaggccttt ggccgcgaac gcgacgtaga cggcgaacag cacgccgagc
     4081 gtcagcagga tgggcagcac gatcaccgtg gcatcgagga cccgtgccga gaagccgctg
     4141 cggtgcgcgc tgccgtagcc cctgcggtac cacgcgcgca ggaacccggc cagggcgatc
     4201 gcgaccaggg ccgagatcag gatggcgaac agctcgagca gtatcgtggg atcggtcagc
     4261 gtggcgaaga atgcctgcac ggcgccgtcg ttcttgttca tcgcgcctcg cgcactgcgg
     4321 tttccgggct aggcattgag atcgggaatc agttcgctct cgaggcgggt gatctggtcc
     4381 ttgaggccga gtttgcggcg cttcagccgc ttcagctgca gttcgtcgac gtggatgtcc
     4441 atcgacaggc gctcgatcac ctggtcgagg tcgcgatgct cgatgcgcag ctgccgcagc
     4501 ttctcgatgt tgcggaacag ctcgcgatcg acgttatctt cgatttcgat catcacgaat
     4561 cggcgatgcg ctcggttacc ggggcgatgc cgtcgggtag cttagcgaag cggcccagcg
     4621 gattccacgg caccgccgga tcgtggaaat cggagccggc ggagcccgcg agcgcgtgcc
     4681 tgcgcgcgag gctcgcgagc cgatcgtgat cgccggggct catgcccgcc atgctcacct
     4741 cgagggcgct gccgccgccc gccgtgaagt ccgtcaccag ctggcgcaac gcgccgccgg
     4801 agaggcgata gcgatgcggg tgcgcgagca ccgtggcccg gcaggattcg acgaggcgcg
     4861 gcaatgcggt atcgagctcg ggccactcga ccggcacatg ccccggccgg tcgcgattca
     4921 gccagcgatc gaaggcgtgc tgggtgtcgt cggcgtagcc cagtgcgacg aggctgcgcg
     4981 cgaggtgcat gcgcgtcggc acgcccccgc cttcgagagt gcgcgcggcg atgtcgcgtc
     5041 ccggaaggcg gctgcgggtg gcgaggcgct cgccgatcgc gagcacgcgc tcgcggcgcc
     5101 gccgcgtgac atcgtcgatc tgcgccgcga tgccggttgc gacggcatcg a
//
LOCUS       DAOQVY010000146         5122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_17953_length_5122_cov_4.810144, whole genome shotgun sequence.
ACCESSION   DAOQVY010000146 DAOQVY010000000
VERSION     DAOQVY010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17953_length_5122_cov_4.810144"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKN91_11235"
     CDS             <1..283
                     /locus_tag="PKN91_11235"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNS28681.1"
                     /translation="IGARGNELARVACTEPREGLWRALTPQMFRLGPLAAALAAAQAA
                     GRQCTDEAQAIEWQGQAPLLVPGSVRNIKVTTPADLALADALLRATMPP"
     gene            297..776
                     /gene="ispF"
                     /locus_tag="PKN91_11240"
     CDS             297..776
                     /gene="ispF"
                     /locus_tag="PKN91_11240"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNS28682.1"
                     /translation="MRIGFGTDVHAYGPGDSVVIAGVRIAHTRGVIAHSDGDVALHAL
                     CDALLGAAGLGDIGMHFPDTDPRWKGADSRELTRSVMALLHARQYAVVNADITILATT
                     PRIGRYRAQMCHGVAQLLGVDEHHVNIKATTTEGLGFIGRSEGLAAQAAVLIDEAAR"
     gene            776..1801
                     /locus_tag="PKN91_11245"
     CDS             776..1801
                     /locus_tag="PKN91_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HNS28683.1"
                     /translation="MAPVVPDVWRTLALDPPRAFGMPPVRGRVRVEPEDFRVDELLGF
                     APAGDGPHLLLHVRKRGANTEWVARELARRAACRPFDVGYAGLKDRHAVTTQWFTVPR
                     GKCSPGEWLGHEGEGYVVLEAHAHTRKLPRGALAGNRFELRIRDIDGDRGALATRLDA
                     LAARGVPDYFGPQRFGRALSNLARVQAGDFRSERAFTLSAARSLVFNAVLARRVAEGS
                     WARIEAGDVANLDGKGSVFAVEAPDEALDARVAALDLHPTGPLWGVGDPRVTGRIAAL
                     EAAVREQFAPVCQALEEEGVAMARRPLRAAVRDVTHVFEGDTLVLQFRLAAGSFATTV
                     LREVLDA"
     gene            1794..2537
                     /locus_tag="PKN91_11250"
     CDS             1794..2537
                     /locus_tag="PKN91_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNS28684.1"
                     /translation="MLETIDEQGVRELRLARPPVNALDTALIESLADAVQSAPAAGAR
                     AIVLSGQPGIFSAGLDVPALLHIDRAGMRSLFEALGRAQEVIARSSVPVIAAITGHAP
                     AGGTVLVLHCDYRVMAEGPFTLGLNEVAVGLTPGALLHHAYARLVGEGRAGVLLTRGA
                     MLDPQYALACGLVDEICAPADVVPRAHAYAQQLLALPSAAMRATRDTARAALIALFDE
                     DDGTFADSATELWFSAETQVRLKALFAKK"
     gene            complement(2577..3110)
                     /locus_tag="PKN91_11255"
     CDS             complement(2577..3110)
                     /locus_tag="PKN91_11255"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNS28685.1"
                     /translation="MSKKPPDDDARLFREAVRDARQLRAEPRATARRRPAARARFANA
                     DRAAVLAESLGPAPPEADVESGEELTFRRPGVQDAVMRKLRRGQYRVEAEIDLHGFTA
                     AEAKLHLRAFLGEAIGSDLRCVRIVHGKGLRSGHRGPVLKYTVNGMLQRIDAVMAFTS
                     ARQVDGGTGAVYVLLAR"
     gene            3178..3939
                     /gene="surE"
                     /locus_tag="PKN91_11260"
     CDS             3178..3939
                     /gene="surE"
                     /locus_tag="PKN91_11260"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNS28686.1"
                     /translation="MKILLANDDGFRAEGIRHLRHALTGVAELTVVAPDRNRSGASNS
                     LTLDVPLRVHEAEDDVLYVVGTPTDCVHLAISGLFNFEFDMVVSGINDGANLGDDVLY
                     SGTVAAAIEGRFLGLTTVAVSLCTEQGGPRHFATAGRVAREIVERLIDQRLDRGMILN
                     VNVPDLPYEQLRGFRATRLGNRHRSEPVIRASDPRGRPVFWIGPAGPGQEAGPGTDFH
                     AISEGFVSVTPLQIDLTRHAALGSVAQWLDGRPRG"
     gene            3962..4594
                     /locus_tag="PKN91_11265"
     CDS             3962..4594
                     /locus_tag="PKN91_11265"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019752402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNS28687.1"
                     /translation="MTSARTRDRLVQRLREQGIRSPEVLDRIRNVPRHIFVDEALASR
                     AYEDTALPIGYGQTISQPYIVARMTEALLEGGPLFNVLEVGTGCGYQTAVLAPLVDKL
                     YTIERIEPLLKRAKERIRELGIKNVRFRHGDGYRGWPAHAPYDGMLVAAAPLRVPDAL
                     LEQLKVGGRLIVPVGAEGEQELVRFTRRELKMERQNLGPVAFVPLLEGTT"
     gene            complement(4854..>5122)
                     /locus_tag="PKN91_11270"
     CDS             complement(4854..>5122)
                     /locus_tag="PKN91_11270"
                     /inference="COORDINATES: protein motif:HMM:NF016326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HNS28688.1"
                     /translation="ISATAIIADWPVRSIAELDEAQLAPLLALDPQVVIIGMPAPAGW
                     PPAAVRARFGARRIGLEVMEFGAACRTYNVLAQEDRQVVLALLP"
BASE COUNT          774 a         1757 c         1789 g          802 t
ORIGIN      
        1 catcggcgcc cgcggcaacg agctcgcgcg ggtggcgtgc accgagccgc gcgagggtct
       61 gtggcgggcg ctcacgccgc agatgttcag gctgggaccg ctcgccgcgg cgcttgcagc
      121 cgcgcaggcc gccggaaggc aatgcaccga cgaggcccag gcgatcgaat ggcagggcca
      181 ggcgccgctg ctggtgccag gcagcgtgcg caacatcaag gtgacgaccc cggccgatct
      241 cgcattggcg gatgccctgt tgcgggccac aatgccccca tgaacggcgc agcggcatga
      301 ggatcggatt cggcaccgac gtgcatgcct acgggcccgg cgacagcgtc gtcatcgcgg
      361 gcgtacgcat cgcgcacacg cgcggcgtga tcgcccactc cgacggtgat gtcgccctgc
      421 atgccctgtg cgacgcgctg ctcggtgcgg cgggactcgg cgacatcggc atgcactttc
      481 ccgacaccga tccgaggtgg aagggtgccg acagccgcga gctcacgcgc agcgtgatgg
      541 ccctgctgca tgcgcgccag tacgcggtgg tcaatgccga catcacgatc ctcgcgacga
      601 cgccgcgcat cgggcgttat cgtgcgcaga tgtgccacgg cgtggcgcaa ctgctcggcg
      661 tcgacgagca ccacgtcaac atcaaggcca cgacgaccga aggcctgggc ttcatcggcc
      721 gcagcgaggg cctggccgcg caggcggccg tcctgataga cgaagcggcg cgctgatggc
      781 gccagtggtt cccgacgtct ggcgcacact cgcgctcgat ccgccgcgtg cattcggtat
      841 gcccccggtg cgcggccgcg tgcgcgtcga gcccgaggat tttcgtgtcg atgagctgtt
      901 ggggttcgcg ccggcaggtg acggaccgca cctgctgctg cacgtgcgca agcgcggcgc
      961 caataccgag tgggtcgcgc gcgaactcgc gcgccgcgcc gcgtgccggc cgttcgacgt
     1021 gggctacgcc ggcctcaagg accgccatgc ggtgacgaca cagtggttca cggtgccgcg
     1081 cggcaagtgc tcgcccggcg aatggctcgg ccacgagggt gaaggctacg tcgtgctcga
     1141 ggcgcatgcg cacacccgca agctgccgcg cggggcactg gcgggcaatc gcttcgaact
     1201 gcgcatccgc gacatcgacg gggatcgagg tgcactcgcc acccggctcg atgctcttgc
     1261 cgcgcgcggc gtgcccgatt acttcgggcc gcagcgcttc gggcgcgcat tgtcgaacct
     1321 cgcgcgcgtg caggccggag attttcgtag cgagcgggcc tttacgctct ccgcggcgcg
     1381 cagcctggtg ttcaacgccg tcctcgcgag gcgcgtggcg gagggcagct gggcgcggat
     1441 cgaggcgggc gatgtcgcga acctcgatgg caagggcagc gtgttcgccg tcgaggcgcc
     1501 cgatgaggca ctcgatgcgc gcgtcgcggc tctcgacctg catccgacgg ggccgctgtg
     1561 gggcgtgggc gacccacggg tcacgggccg catcgcggcg ctcgaggccg cggttcgcga
     1621 gcagttcgcg ccggtgtgcc aggcgctcga agaggagggc gttgcgatgg cgcgccggcc
     1681 gctgcgggcc gcggtacgcg atgtcacgca tgtcttcgaa ggcgacacac tcgtactaca
     1741 attccggctc gcggcgggca gttttgccac gaccgtgctg cgtgaggtgc tcgatgcttg
     1801 agacaatcga tgaacagggc gtgcgcgagc tgcgcctcgc gcgccctcct gtcaatgcgc
     1861 tcgacaccgc gctgatcgag tcgctggccg atgcggtgca gtccgcgcca gccgccggcg
     1921 cgcgcgccat cgtgctgtcg ggccagcccg gcatcttctc ggcgggcctc gacgtgcccg
     1981 cgctgttgca catcgatcgc gccgggatgc ggagcctgtt cgaggcgctg ggtcgcgcgc
     2041 aggaggtgat tgcccgctca tcggtgccgg tcatcgccgc catcacgggg catgcgccgg
     2101 cggggggcac ggtgctcgtc ctgcattgcg actatcgcgt gatggccgag gggcctttca
     2161 ccctgggcct caacgaggtt gcggtcgggc tcacgcccgg cgcgctgctg catcacgcgt
     2221 acgcgcgcct cgtcggcgag ggtcgtgccg gcgtgctgct gacgcgcggc gcgatgctcg
     2281 atccgcagta cgcgctagcc tgcggtctcg tcgacgagat ctgtgcgccc gccgacgtcg
     2341 tgccgcgtgc gcacgcctat gcgcagcagc tgctggcgct gccctcggcg gcgatgcgtg
     2401 cgacccgcga tacggcgcgc gcggcactca ttgcgctgtt cgacgaggac gacgggacct
     2461 tcgccgacag cgccacggag ctgtggttct cggcggagac ccaggtgcgc ctcaaggcgc
     2521 tgtttgccaa gaaatgagca gccacgcgcg tgcggcagcg gccacactgc ccgtcgtcag
     2581 cgtgcgagca gcacgtagac cgcgcccgtg ccgccatcga cctggcgcgc cgaggtgaag
     2641 gccatcaccg cgtcgatccg ctgcagcatg ccgttcacgg tgtacttgag caccggccca
     2701 cgatgcccgg agcgcaggcc cttgccgtgc acgatgcgca cgcagcgcag gtcggaaccg
     2761 atcgcctccc cgaggaacgc acgcagatgc agcttcgctt cggctgcagt aaagccatgc
     2821 aggtcgatct cggcttcgac ccgatactgg ccacgacgca acttgcgcat cacggcgtcc
     2881 tgcaccccgg ggcggcggaa ggtgagttcc tcgccgcttt cgacgtccgc ctccggcggc
     2941 gcgggcccga gcgattcggc gagtaccgcc gcacggtccg cgttcgcgaa gcgcgcacgc
     3001 gccgccgggc gccggcgggc cgtggcgcgc ggctccgccc gcagttgccg cgcatcgcgc
     3061 accgcttcgc ggaacagccg tgcgtcgtcg tcgggcggtt ttttcgacac gatttcctcc
     3121 accggaacgc tttcagtata ttgacgcgcc cgaaaattcc gaaagtcccc gcgccgagtg
     3181 aagatcctgc ttgccaatga cgacggtttt cgcgccgagg gcatccgcca cctgcggcac
     3241 gccctgaccg gtgtcgccga gctgacggtc gtggcgcccg accgcaaccg cagcggcgcc
     3301 agcaattcgc tcacgctcga tgtaccgctg cgcgtgcacg aggccgagga cgacgtactg
     3361 tatgtggtcg gcacaccgac cgattgtgta cacctcgcca tttcgggcct gttcaatttc
     3421 gagttcgaca tggtcgtctc gggcatcaac gacggcgcca acctcggcga cgacgtgctc
     3481 tactccggca cggtcgccgc ggcgatcgaa ggccgtttcc tcggtctcac caccgtggcg
     3541 gtttcgctgt gcacggagca gggcggtccg cgccacttcg cgaccgcggg ccgcgtcgcg
     3601 cgcgaaatcg tcgagcggct gatcgaccag cggctcgacc gcggcatgat cctcaacgtc
     3661 aacgtgccgg acctgcccta cgagcagcta cgcgggtttc gcgccacgcg cctcggtaac
     3721 cggcatcgtt ccgagccggt gatccgcgcg agcgacccgc ggggccggcc ggtgttctgg
     3781 atcggtccgg ccggccccgg gcaggaagcg gggccgggta ccgatttcca cgccatcagc
     3841 gagggcttcg tgtcggtcac gccgctgcag atcgacctga cgcgccatgc ggcgctcggc
     3901 agcgtggctc agtggctcga cgggcggcca cgtggctgac gtgcgcttct ccggcatcgg
     3961 catgacttcc gcacgcaccc gcgaccggct ggtgcagcgc ctgcgcgaac agggcatccg
     4021 cagtcccgag gtactcgacc gcatccgcaa tgtgccgcga cacatcttcg tcgacgaggc
     4081 gctcgccagc cgtgcctacg aggacacggc gctgccgatc ggttacgggc agacgatttc
     4141 gcagccctac atcgtcgcgc gcatgaccga agcgctgctc gagggcgggc cgctgttcaa
     4201 cgtactcgag gtcggcaccg gctgcggcta ccagaccgcg gtgctcgcgc cgctggtcga
     4261 caagctgtac acgatcgagc gtatcgagcc gctcctgaag cgtgccaagg agcgtatccg
     4321 tgagctcggc atcaagaacg tgcgcttccg ccacggtgac ggctatcgcg gctggccggc
     4381 gcacgcaccc tacgacggca tgctggtggc cgcagctccg ctgcgcgtgc cggacgcgct
     4441 gctcgagcag ctgaaggtcg gcgggcgcct gatcgtgccg gtcggcgcgg agggtgagca
     4501 ggagctcgtg cgcttcacgc ggcgcgaact gaagatggag cgccagaacc tcggaccggt
     4561 ggccttcgtg ccgctgcttg agggaaccac atgagaaata ctcgctaata cactgattgt
     4621 cagaatattt taagctcgct tgccatcatc gtgcgcgcgt ggctaacatg tcgcgcaatc
     4681 tggcgcatcg cgagaagaca cagggattta cgggggagtc ggcgtagaag aaatagaacg
     4741 aagaagaaaa agaacagacg acgacaatcg gccgggtctg tcagctgcgg acgattgggg
     4801 tgattacgag ctttccgccc tgtgtcttct ctcgatgcgc ccgaccgggc gactcacggc
     4861 agcaatgcca gcacgacctg ccgatcctcc tgtgccagca cattgtaggt gcggcacgct
     4921 gcgccgaatt ccatgacctc gagaccgatg cgccgggcac cgaagcgtgc gcgcaccgcc
     4981 gccggcggcc agcctgccgg tgccggcatg ccgatgatca cgacctgggg atcgagcgcg
     5041 agcagcggcg cgagttgcgc ctcgtcgagc tcggcgatcg agcgcacggg ccagtcggca
     5101 atgatcgccg tcgcgctgat ca
//
LOCUS       DAOQVY010000147         5089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_18120_length_5089_cov_4.843464, whole genome shotgun sequence.
ACCESSION   DAOQVY010000147 DAOQVY010000000
VERSION     DAOQVY010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18120_length_5089_cov_4.843464"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PKN91_11275"
     CDS             complement(<1..291)
                     /locus_tag="PKN91_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28689.1"
                     /translation="MLVGDAITACGLSLEQPDGDLIVVALASDGRLIHRSAPEVAARN
                     LGANLYQAGGVFRSGSVSGSRGRNADNLVRIVWLAFDADLSDYLGWQKADLWV"
     gene            complement(298..810)
                     /locus_tag="PKN91_11280"
     CDS             complement(298..810)
                     /locus_tag="PKN91_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28690.1"
                     /translation="MIAPFTVTNSSTGGAPRDLTRKVGNELKLVPVDRENDGYGCPDP
                     KRYKLILSGMSEQFEEEDRYNPGEMKQKVVCEFTVVGNRKWEGTRFSSFYNIPKDWTD
                     ERAYLGHLAAALKGKARLDKGDAVDFPKHILNETVFEAVIEVATSARGYDYPKIVTYL
                     ALDNDDAFPE"
     gene            complement(1061..1300)
                     /locus_tag="PKN91_11285"
     CDS             complement(1061..1300)
                     /locus_tag="PKN91_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNS28691.1"
                     /translation="MATNRATRYRATTLSDVIDAQGRIRRWVAAQAGIDQPTLSHLEK
                     GRRTIPADVADRIAMALQTPMLDLFEPVVGDDDGV"
     gene            complement(1641..1898)
                     /locus_tag="PKN91_11290"
     CDS             complement(1641..1898)
                     /locus_tag="PKN91_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28692.1"
                     /translation="MNAVTDGATDAQNDFIEGLRKQLHISRALLSSHTQRAYGHPVEA
                     LTKRDASLLIDQMLSWREGGKGLPADLQRAAGQADLPGFGS"
     gene            complement(1895..2071)
                     /locus_tag="PKN91_11295"
     CDS             complement(1895..2071)
                     /locus_tag="PKN91_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28693.1"
                     /translation="MATPVIELECFLALSESLPLGLSAIDIDSVDLAIANATELLDML
                     TDLLAVVEAMEDAQ"
     gene            complement(2463..3446)
                     /locus_tag="PKN91_11300"
     CDS             complement(2463..3446)
                     /locus_tag="PKN91_11300"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNS28694.1"
                     /translation="MVALGLAQEITNDYAIYNGDCLEIMPSLPDGSIHLSVYSPPFAG
                     LYQYSSSERDLSNARTYAEFLEHYEFVVRELHRLTMPGRMSAVHCMDVPSGNTGKGDK
                     LKDFPGDLVRLHDRLGFDYVARYHVWKEPLAVRNRTMRKSLAHQQVVEDATKCDNASA
                     DYLLLFRKHGENPVPVEHPNGFLTYAGERQIPADVLRYKGWDGKQIENRYSHWIWRQY
                     ASAFWDDVRIDRVLPFREARDEEDEKHVHPLQLDVIDRIVMLFSNPRERVLTPFMGVG
                     SEVYGAVANDRIGIGIELKPSYYRQAVKNVAAALEQRPETLPLFAGIEVAS"
     gene            complement(3428..4813)
                     /locus_tag="PKN91_11305"
     CDS             complement(3428..4813)
                     /locus_tag="PKN91_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNS28695.1"
                     /translation="MSDYLTFLERKTQLDGDHGFDPIVMPDALFDFQQSLVTWAVRKG
                     RAAILADCGLGKTLMELAWAQNVSIHTGKPVLILTPLAVAGQFEQEAHRFGFDATHSR
                     RGEIEAPIVIANYERLHLFDADRFGGIVCDESSILKSFDGMRRRDITEVMRTKPYRLL
                     STATAAPNDFIELGTSSEALGYLGYLDMLSRYFVNDSNNNLHQRRPGRFRTEGNWRFK
                     GHAEQPFWRWVASWARAMRRPSDLGFSDERFVLDPLIVNEHVVEARSAAPGMLFALPA
                     NGLREEREERRRTLDERCEMVAELVNDTGQPAVVWCHLNDEGDLLERLIPDGKQVSGK
                     DTDERKEATFDSFSRGELRVLITKPKIGAWGLNWQHCNHLTYFPSHSYEQYYQAVRRC
                     WRFGQTRPVTVDIVATEGEVNVQQSLQRKSEQADQMFTELVAHMTDAMGVRRLAEFDT
                     PMEVPQWLLSA"
BASE COUNT          905 a         1721 c         1462 g         1001 t
ORIGIN      
        1 cacccagaga tcggcttttt gccagccgag gtagtcggag agatcggcgt cgaacgccag
       61 ccagacgata cggaccagat tgtccgcatt gcggccccgg ctgcccgaca ccgacccgct
      121 ccgaaagacc ccccccgcct ggtagagatt ggccccgagg ttgcgtgcgg caacctcggg
      181 tgcgctgcga tggatcagtc gcccgtcgct cgctagtgca acgacgatca ggtctccgtc
      241 aggttgttcg agtgacagcc cacaggccgt gatcgcgtcg ccgaccagca tggccgccta
      301 ctccgggaat gcgtcgtcgt tgtcgagcgc caggtaggtc acgatcttgg gatagtcgta
      361 tccgcgcgcc gacgttgcga cctcgatcac cgcctcgaaa acggtttcgt tcagaatgtg
      421 cttcgggaag tccaccgcgt cgcccttgtc gagccgggcc ttgcccttca gtgctgcagc
      481 cagatgcccg aggtaggcgc gttcgtcggt ccagtctttc gggatgttgt aaaacgaact
      541 gaatcgcgtt ccctcccatt tcctgttgcc caccacggtg aactcgcaga ccactttctg
      601 cttcatctcg ccggggttgt atcggtcctc ctcctcgaat tgctcggaca taccggagag
      661 aatcagcttg tagcgtttgg ggtcggggca gccgtagccg tcgttctcgc gatccaccgg
      721 cacgagcttg agttcgttac ccaccttgcg cgtcagatcg cgaggcgctc cgcctgtcga
      781 ggaattggtc actgtgaatg gcgcaatcat ggtttcgtgt gctccctatc tcatcgagcg
      841 gccacgtgtc ggccgcacat ccgattcgtc tgccacggct tgcacgtccg tagccccgcc
      901 tgattgcatc gttgcgccca gcgcgaaatt gatcacatcc tcggaatacc cgccatcggc
      961 aagctcccgc agtgcctccc ggtacgccgc gcgttgcgcg ctccggctct cgtacgaacc
     1021 gatcaacacc ggcccgtcgc atcgcgtgat cgcgtatatc tcatacgcca tcatcatcac
     1081 ccaccacagg ctcgaataaa tcgagcatcg gcgtttgcag cgccatggca attcgatccg
     1141 ccacatccgc cggaatcgtg cgtcgtccct tttccaggtg cgagagcgtc ggctgatcga
     1201 tccccgcttg cgcggccacc catcgccgaa tacgcccctg tgcatcgatc acgtcggata
     1261 atgttgtcgc ccggtatcgg gtcgcacgat tcgttgccat gcgttgattc tacaccccag
     1321 cgcctgcgat tgcaagatgt gttgcatctc gactcatcgg ctcagccatc ccttgcgcac
     1381 acctgccgcg agcatggcca gctgccgccg ccggtcacgc cgctgcgcgt gtcgctgtcg
     1441 ccacacagct agccgtgtca gccgttcgac gctgtgtttg ttggccgctg ccaacgcccg
     1501 gtcaccaggc agatatggcg acgcataccg gtctcgttgc cggccgggcg cacaacccag
     1561 gtgataggtc accccgcccc ccgtcgctgt gcgatccacg gacaagatgg tgagttggca
     1621 cgttggacaa actggctggc tcatgatccg aaccccggca gatcagcttg tcccgccgcg
     1681 cgctgcaagt ccgctggcag tcccttaccg ccctcgcgcc acgacaacat ttggtcgatc
     1741 aacagactcg cgtctcgttt cgtgagcgcc tcgaccgggt gtccataggc gcgttgcgtg
     1801 tggctactga gcagcgccct cgaaatgtgg agctgcttgc gcaacccttc gatgaaatcg
     1861 ttctgcgcat cggtcgcgcc atctgtcacc gcattcattg cgcatcctcc atcgcttcca
     1921 cgactgcgag gagatcggtc agcatgtcca gcagttcggt cgcattggcg attgccagat
     1981 cgaccgaatc gatatcgatc gccgaaagac cgagcggcaa cgactcgctc aacgccaaga
     2041 agcactccag ttcgatcacg ggtgtcgcca tcgattcgtt tgtcatttgc tacaatctcc
     2101 ccgagccttc tccaagctcc attccctctc cgcccggcga tcctacccgc cgggcggact
     2161 catgctacgc ggcgacggac atcgtcaccg tggaggccgc agccgctgac cccccgttac
     2221 aacccgcgcg gccacggact ccacggtgac gccggatcgc tccagcgccc cgccggaaat
     2281 agcacgctcc tgtggttcca cccaaaaact gagcgtgcaa tcgattggcg cactccgcgc
     2341 ctgggagccg ccgcaatccg ttgggagggg catcgtgttt ccacgacatg gcagcggctc
     2401 ccaggcgcgg ccccggccgg agccggcaca gatcgcgtag acgtggtagg gggtaggggc
     2461 ggtcatgacg cgacctcgat gccggcgaag agcggcaggg tttcgggtcg ctgttcgagt
     2521 gccgcggcga cgttcttgac cgcctgccgg tagtagctcg gcttgagctc gatcccgatc
     2581 ccgatgcggt cattcgccac cgcaccgtag acctcgctcc caacgcccat gaatggcgtg
     2641 agcacccgtt cgcgggggtt gctgaagagc atcacgatcc ggtcgatcac atcgagctgc
     2701 agcggatgga cgtgcttttc gtcctcttcg tcgcgtgcct ctcgaaacgg cagcacccga
     2761 tcgattcgca cgtcatccca aaaggcagac gcgtattgcc tccaaatcca atgggagtag
     2821 cggttttcga tctgcttccc gtcccagccc ttgtagcgca acacgtcggc tggtatctgc
     2881 cgctctccgg cataggtcag aaacccgttc ggatgttcca ccgggaccgg attctctccg
     2941 tgcttgcgga agagcagcag gtagtccgcg ctggcgttgt cacacttggt cgcatcctcg
     3001 accacctgct gatgcgccag actcttgcgc atggtccggt tgcggaccgc cagtggctcc
     3061 ttccaaacgt ggtagcgcgc cacgtaatcg aatcccaatc ggtcatgcag tcgcaccaga
     3121 tcgccgggga agtctttgag cttgtcgccc ttgccggtgt tcccgcttgg cacgtccatg
     3181 caatgcacgg cgctcatccg gcccggcatg gtcagccggt gcagctcccg cacgacgaat
     3241 tcgtagtgct cgaggaactc ggcataggtc cgggcattcg acagatcgcg ctccgaactg
     3301 ctgtattggt agagtcccgc aaacggtggc gagtagaccg atagatgaat cgatccgtcc
     3361 ggcagcgatg gcatgatttc caggcaatcg ccgttataga tcgcgtagtc gtttgtgatc
     3421 tcctgcgcta ggccgagagc aaccattgcg gaacctccat tggggtatcg aattcggcca
     3481 gccgccgcac gcccatcgca tcggtcatgt gcgcgaccag ttcggtaaac atttgatcgg
     3541 cttgctcaga cttacgctgc aacgattgct gcacgttgac ctcgccttcg gtcgcgacga
     3601 tatccaccgt gaccggccgg gtttgcccga accgccagca ccgtcgcacg gcttggtaat
     3661 actgctcata gctatggctg gggaaatagg tcaggtggtt acagtgctgc cagttgagcc
     3721 cccacgcgcc gatcttgggc ttcgtgatga gcacccgcag ctcgccgcgg ctgaacgaat
     3781 caaacgtcgc ctctttgcgc tcgtcggtgt ccttgccgct gacctgcttc ccgtccggga
     3841 tcaggcgttc caacaggtcg ccttcgtcgt tcagatgaca ccagacaacg gccggctgcc
     3901 cggtgtcatt caccagttcc gcgaccatct cgcagcgctc atcgagcgta cggcggcgct
     3961 cctcgcgttc ctcgcgcagt ccattggccg gaagcgcgaa cagcattccc ggcgcggccg
     4021 agcgcgcctc caccacatgc tcattgacga tcaatggatc cagtacgaag cgttcgtcac
     4081 tgaatcccag gtcggacggc cggcgcattg cacgcgccca gctcgccacc cagcgccaga
     4141 acggttgctc tgcgtgccct ttgaatcgcc agttgccctc ggtgcgaaat cggcccggtc
     4201 gccgctgatg caggttgtta ttgctgtcat tcacgaagta gcgtgacagc atgtccagat
     4261 agccgagata cccaagcgcc tcgctcgacg tgcccagctc gatgaaatca ttcggcgcgg
     4321 ccgtcgcggt ggacagcaac cggtacggct tggtgcgcat cacttcggtg atgtcgcgcc
     4381 gccgcatccc atcgaacgat ttcaggatgc tggactcgtc gcagacaatc ccgccaaatc
     4441 ggtccgcatc gaagaggtgc aaccgttcgt agttggcaat gacgatcggt gcttcgatct
     4501 cgccccggcg actgtgagtt gcgtcgaatc cgaagcggtg tgcttcctgc tcgaactgcc
     4561 cggccaccgc aagcggcgtc agaatcaaca ccggcttccc ggtatggata gaaacgttct
     4621 gcgcccatgc caattccatg agcgtcttgc cgagtccgca atccgcgaga atcgcagccc
     4681 gtcccttgcg aaccgcccag gtaacgagcg attgctggaa atcgaacagc gcgtccggca
     4741 tcacgatcgg atcgaacccg tggtcgccat cgagctgcgt tttccgttcc aggaacgtga
     4801 ggtaatcgct catgaccgta ctccgtacac atagagcgag ccatcgacat cacaaagaag
     4861 tccgtacccg cgttgccaga catcccagcg cgcgcgcgca tgcgcgacgg tcgcgatatc
     4921 gagatcgagc ccgtcacaaa tgtggacagc accgacatag agcgccgagg cccacgccga
     4981 ggcccgcgcc gaggcccccg ccgagccccc cgccgagtcc cgcgccgagt cccgcgccga
     5041 ggcccacgcc gaggcccacg ccgaggccca cgccgaggcc cacgccgag
//
LOCUS       DAOQVY010000148         5087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_18132_length_5087_cov_5.614865, whole genome shotgun sequence.
ACCESSION   DAOQVY010000148 DAOQVY010000000
VERSION     DAOQVY010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18132_length_5087_cov_5.614865"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PKN91_11310"
     CDS             <1..457
                     /locus_tag="PKN91_11310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS28696.1"
                     /translation="VAGAIGGVAGGVLDRRYGSKRTLIGSLAIMLAVSIVMLLELPAV
                     TVRGADAALFASPGDRIFLALAIVASACLGAILASSRSMMVRLSPADRLGEFFGLYVM
                     VGRASSFAAPLLVALATTAFGNQTTGVFSVALTFMALGLVLLGRVHDAR"
     gene            444..1745
                     /locus_tag="PKN91_11315"
     CDS             444..1745
                     /locus_tag="PKN91_11315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS28697.1"
                     /translation="MMRADRASPPWPTARLAWGTVIALTLVYAIAFVHRIGLGLLVEP
                     LQKDLHFSDTAIGWLAGILFAVPYTLGGPIFGWFADRRSRRSLIALAALGWSATTGAF
                     GLLTSYTALAATRIAAGLTQSALQPAASSMIADCFQARDRSRAYGVYVAGTAFGTALA
                     YWLGAVAIGLGAWLAMRTGMKDWSAAFLALALIGLIAPLTMLFVREPARQERAGEAAT
                     SVRATVSFIRREWLVVGSLSLGVAICFLAPYGQLAFMPVMFSRKYGWSAAELALVYGV
                     VAVFAGGFGSMLGGWISAWLARRGRVDSDWIICTTGAAGSLVTGGIAPLMPTAEWSLF
                     FFTLSGLFTNWPSVGALGAVNRLAPNEMRGQLTSVYTSSVGLIGAGLGPVAIGTLSDR
                     LPPAWGGVSAAMSVTFFGCAVLSCALLVVGRAPFARLVSRA"
     gene            1811..3148
                     /locus_tag="PKN91_11320"
     CDS             1811..3148
                     /locus_tag="PKN91_11320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS28698.1"
                     /translation="MQNPEFDRSRYPRAGYAWYVVAVLLVAYILAFVDRDVISLLVQP
                     IRADLGISDLQMSFLLGGAFALFYTFFGMPIAWLADRWNRRLIIFFGVTLWSVATVLC
                     GMAGGYWSLFAARVGVGVGEAALNPPALSLLKDYFPPDRLGRAVGVYTAGISAGMGVG
                     SFLAATIYPAISAHGPYLLPVFGTVSPWQYMFILVGLPGFLVALMILTIREPSRRVYA
                     TTFERVSLWQSIAYVGQRRSTYGLLFIGLSAMAITNYGVGYWIPEFLRRTYELDIPTM
                     AWYLQVRGVVLIVAGVVGVLFGGWLSDTLNRRYDDGYVRTAMIGYAFLFVGYVGIPLM
                     PTPGLSMAMLVPATIGGAIATVGGVASVLAVAPPNMRAQLTAMYYFTLNLIGLMLGPT
                     MVALLTDRVFRSDDSLYLSLVIVAGASVAGGTWLLWRARGHYGASMREARSWQ"
     gene            3165..3833
                     /locus_tag="PKN91_11325"
     CDS             3165..3833
                     /locus_tag="PKN91_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28699.1"
                     /translation="MTLYELVLWLHVLVMGYWIGSDLVINALTHYTTSASGLSGVERK
                     RLWEFLLEVDQHPRNALIFSVPLGFTLASILGLVPIHGAGLVVVWIVCAVWFWFMWVT
                     HWKRNAPEGRAWARWDWWLRYVLIALAVGSGVLSLATGKPFAAPWLAWKVILFGGVLC
                     AGIGIRYYIRKAYAAWPRIWAEQAEPGDEAFVHKSLWQGTYVLWVLWALLFIIGWLGA
                     AKPG"
     gene            complement(3843..4370)
                     /locus_tag="PKN91_11330"
     CDS             complement(3843..4370)
                     /locus_tag="PKN91_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28700.1"
                     /translation="MSNPVSQDEAEICAHFASYSRAWRANDVAALLGHWAEPEFRFYK
                     AEEAERFFTRFADVAAYFRGNAELIEIMDVEFAVNELIPCSAGMVFAIAQLQWNIRFA
                     RDARLPDGSPYAHRGKAMGGDNHVLALLTHMPAGWRFAGWSETPDAPLSYISRLYRKN
                     VRPGSPAAVTADGAP"
     gene            complement(4380..>5087)
                     /locus_tag="PKN91_11335"
     CDS             complement(4380..>5087)
                     /locus_tag="PKN91_11335"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNS28701.1"
                     /translation="DGADSPTDIGPKALDFYVADPILPRVAEIERAGYKFRSRPVRHQ
                     VGRAESEECLFTGPDGVPILLMVGHRHSDAELRPGCLTGPYSEIATISIVSGDLEASR
                     VFYEDVLGMALIVDAETGQSHRNSVNDLTGVPPDTRVHFKVYAEAGEASGKVLIVHFF
                     ERTGRRLLDRMRPGHLGFSLMSHACDDLDTLARRLAAGGFAIVAPPTRIRTDHDERRA
                     LLARGPNEELLEFIQAS"
BASE COUNT          673 a         1653 c         1756 g         1005 t
ORIGIN      
        1 ggtggccggt gcaatcggcg gtgtggccgg gggcgtgctc gaccgccgct acggctcgaa
       61 gcgcaccttg atcggatctc tcgctatcat gctggccgtg tcgattgtca tgctgctcga
      121 attgccggca gtcacggtgc gcggcgccga cgcggcgttg ttcgcatcgc cgggagaccg
      181 catcttcctg gcccttgcga tcgttgccag tgcctgcctc ggtgcgatcc tcgcgagcag
      241 ccgctcgatg atggttcgcc tctcgccggc cgatcgcctg ggcgagtttt tcgggcttta
      301 cgtcatggtg ggacgtgcct cgagctttgc ggccccgttg ctcgtcgcgc tcgcgacgac
      361 cgcattcggc aatcagacga ctggtgtatt cagcgtggcg ctgaccttca tggcgctggg
      421 cctcgtgctg cttggcaggg tgcatgatgc gcgctgaccg cgccagcccg ccctggccga
      481 ccgcgcgatt ggcgtggggc acggtcatcg ccctgacgct cgtctacgcg atcgccttcg
      541 tgcaccgcat cgggctcgga ctgctcgtcg agccgctgca gaaggacctg cacttcagcg
      601 acacggcgat cggctggctc gcgggcatat tgttcgccgt gccttatacg ctcggcgggc
      661 cgatcttcgg ctggttcgca gatcggcgca gccggcgctc gctgatcgcg ctggcggcgc
      721 tcggctggag cgcaacgacc ggcgccttcg ggctgctgac gtcctacacg gcgctcgccg
      781 ccacgcgcat cgccgcgggc ctcacgcagt cggccctgca accggcggct tcgtccatga
      841 ttgccgactg cttccaggcg cgcgatcgct cgcgcgccta tggcgtctac gtcgccggca
      901 cggcgttcgg taccgcgctc gcttactggc tcggcgcggt ggcgatcggc ctcggcgcat
      961 ggctcgcgat gcgcaccggc atgaaggact ggtccgctgc attcctggcg ctcgccctga
     1021 tcggcttgat cgcgccgctg accatgttgt tcgtgcgcga acccgcgcgg caggagcgcg
     1081 cgggggaggc ggccacgagc gttcgcgcga ccgtcagctt catccgccgc gaatggctgg
     1141 tcgtcggcag cctgagcctc ggcgtcgcga tctgtttcct tgccccttac ggccagctcg
     1201 cgttcatgcc ggtgatgttc tcgcgaaaat atggctggag tgccgcggag ctcgcgctcg
     1261 tctatggcgt ggtggcggta tttgccggcg gattcgggtc catgctcggt ggctggatct
     1321 cggcctggct cgcgcgtcgc gggcgggtcg acagcgactg gatcatctgc accacgggtg
     1381 cggcgggcag cctcgtcacc ggcggcatcg cgccgctgat gccgactgcc gagtggagcc
     1441 tgttcttctt cacgctctcg ggcctcttta cgaactggcc ttcggtggga gcgctcggcg
     1501 ccgtgaatcg tctcgcgcca aatgaaatgc gcggccagct cacctcggtc tatacctcga
     1561 gcgtggggct gatcggcgcg ggcctcggcc cggttgccat cggcacgctg tccgaccgcc
     1621 tgccgccggc ctggggcggg gtgtccgcag cgatgtcggt gactttcttc ggctgtgcag
     1681 tgttgtcctg cgcgctgctg gttgtcgggc gcgcgccgtt tgcgcggctc gtctcgcgcg
     1741 catagccgcg tgaaaaaaac aactttgtgt aactatacgg gcccccgact ttcggcgagg
     1801 aaaggtgcca gtgcagaatc ctgagttcga ccgctcccgg tacccgcgtg ccggctatgc
     1861 ctggtatgtg gtcgcagtac tgctcgtcgc atacatcctg gcgttcgtcg accgcgacgt
     1921 gatctcgctg ctggtgcagc cgatccgcgc cgacctcggc atcagcgacc tgcagatgag
     1981 ctttctgctg ggcggggcgt ttgcgctctt ctacactttc ttcggcatgc cgatcgcctg
     2041 gctcgccgac aggtggaacc ggcgcctgat cattttcttc ggcgtgaccc tgtggagcgt
     2101 cgccaccgtg ctgtgcggca tggccggcgg ctactggtcg ctgttcgcgg cgcgcgtcgg
     2161 ggtcggggtc ggtgaggcgg cgctcaatcc gcccgcgctg tcgttgctga aggactattt
     2221 cccgcccgat cgcctcgggc gcgcggtcgg cgtgtacacg gccggcattt ccgccggcat
     2281 gggcgtcggc agcttcctcg ccgcgaccat ctatcctgcg atcagcgcac acggacctta
     2341 cctgctgccg gtgttcggca ccgtgagccc gtggcagtac atgttcatcc tcgtcggcct
     2401 gcccggcttc ctcgtggcac tgatgatcct gacgatccgc gagccctcgc gccgtgtcta
     2461 tgcgacgacc ttcgagcgcg tgtcactgtg gcagtcgatc gcctatgtgg ggcagcgtcg
     2521 cagcacctac ggcctgctgt tcatcggcct ctccgccatg gccatcacca actacggcgt
     2581 cggctactgg attcccgagt tcctgcgccg cacgtacgag ctcgacatac cgaccatggc
     2641 ctggtatctg caggtgcgcg gcgtcgtgct gatcgtcgcc ggcgtggtgg gcgtgctgtt
     2701 cggcggctgg ctcagcgata ccctgaatcg ccgctacgac gatggctatg tccgcacggc
     2761 catgatcggc tacgcgttcc tgttcgtcgg ctatgtcggc atcccgctga tgccgacgcc
     2821 gggtctttcc atggccatgc tcgttccggc gaccatcggc ggcgccatcg cgaccgtggg
     2881 gggcgtggca tcggtactgg cggtcgcgcc gcccaacatg cgtgcgcagc tcacggccat
     2941 gtactacttc acgttgaatc tgatcggcct gatgctgggt ccgaccatgg tggcgctgct
     3001 caccgatcgg gtgttccgct ccgacgattc gctgtatctt tcgctcgtca tcgtggcggg
     3061 cgcgagcgtc gcgggcggca cgtggctgct gtggcgcgcc cgcggtcact atggcgcgag
     3121 catgcgtgag gcgcgaagct ggcaatgaaa accgggaaga cagaatgaca ctatatgaac
     3181 tggtcctgtg gctgcatgtg ctcgtgatgg gctactggat cggcagcgat ctcgtgatca
     3241 acgcgctgac ccattacacg accagcgcgt cgggcctatc gggcgtcgag cgcaagcggc
     3301 tgtgggagtt cctgctcgag gtcgatcagc atccgcgcaa tgcgctgatt ttcagcgtgc
     3361 cgctcgggtt cacgctcgcc tcgatcctcg ggctcgtgcc gatccatggc gcggggctcg
     3421 tggtcgtatg gattgtctgc gcggtctggt tctggttcat gtgggtcacg cactggaagc
     3481 gcaatgcgcc cgaggggcgc gcctgggcgc gctgggactg gtggttgcgt tacgtgctga
     3541 tcgccctggc ggtcggcagc ggcgtactct cgctcgcgac cggcaagccg tttgcggcgc
     3601 cgtggctcgc ctggaaggtg atcctgttcg gcggcgtgct gtgcgcgggg atcggcatcc
     3661 gctattacat ccgcaaggcc tacgcggcct ggccgcgcat ctgggcggag caggccgagc
     3721 cgggtgacga ggcattcgtg cacaagtcgc tgtggcaggg cacttacgtg ctgtgggtgc
     3781 tgtgggcgct gctgttcatc atcggctggc tgggagctgc gaaacccggc tgaggctggc
     3841 gcctatggtg cgccgtccgc agtgacagcc gccggggatc cgggccgcac gttcttgcga
     3901 tagaggcgcg agatgtagct gagcggcgca tccggcgttt cgctccagcc ggcaaagcgc
     3961 cagccggcgg gcatgtgcgt gagcagcgcg agcacgtggt tgtcgccacc catcgccttg
     4021 ccgcggtggg cgtacggcga tccgtccggt agccgggcgt cgcgcgcgaa gcggatgttc
     4081 cactgcagct gcgcgatggc gaagaccatc ccggcgctgc agggaatcag ttcgttcacg
     4141 gcgaactcca cgtccatgat ctcgatgagt tcggcattgc cgcggaaata cgccgccacg
     4201 tccgcgaagc gggtgaagaa gcgctcggct tcctctgcct tgtagaaccg gaactccggc
     4261 tcggcccagt ggccgagcag ggcggccacg tcgttggccc gccatgcccg cgaataactt
     4321 gcgaagtgcg cgcagatttc ggcctcgtcc tgtgaaacgg gattcgacat gcggcggcct
     4381 cagcttgcct ggatgaattc gagcagttcc tcgttcggac ccctggcgag cagcgcgcgg
     4441 cgctcatcgt gatccgtgcg gatacgggtc ggcggcgcga cgatcgcaaa gccacccgcg
     4501 gcgaggcgtc gtgccagcgt gtcgagatcg tcgcaggcat gactcatcaa tgagaatccg
     4561 aggtggccgg ggcgcatgcg atcgagcagg cgccgcccgg tgcgttcgaa gaaatgcacg
     4621 atgagcacct tgccactggc ttcgccggcc tccgcgtaga ccttgaagtg cacccgggtg
     4681 tcgggcggca cgccggtcag gtcgttcacc gaattgcgat gtgactggcc ggtctcggcg
     4741 tcgacgatca gcgccatgcc gagcacgtcc tcgtagaaga cccggctcgc ctcgaggtcg
     4801 cccgacacga tcgagatggt ggcgatttcg ctgtagggcc cggtgaggca gcctgggcgc
     4861 agttcggcat ccgaatggcg atggcccacc atcagcagga tcggcacgcc gtccggtccg
     4921 gtgaagaggc actcctcgct ctcggcgcgg ccgacctgat ggcggaccgg acgggaacgg
     4981 aacttgtacc ccgcgcgttc gatctcggcg acccgcggca gtatcgggtc ggcgacatag
     5041 aagtcgagcg ccttggggcc gatgtcggtg ggtgaatccg cgccgtc
//
LOCUS       DAOQVY010000149         5066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_18253_length_5066_cov_7.154460, whole genome shotgun sequence.
ACCESSION   DAOQVY010000149 DAOQVY010000000
VERSION     DAOQVY010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18253_length_5066_cov_7.154460"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..2166)
                     /gene="fusA"
                     /locus_tag="PKN91_11340"
     CDS             complement(79..2166)
                     /gene="fusA"
                     /locus_tag="PKN91_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNS28702.1"
                     /translation="MPRTTPIERYRNIGISAHIDAGKTTTTERILFYTGVSHKLGEVH
                     DGAAIMDYMEQEQERGITITSSATTCFWKGMDKSFPEHRINIIDTPGHVDFTIEVERS
                     LRVLDSAVALFDSVAGVQPQSETVWRQMNRYNVPRIAFVNKMDRAGANFLRVVDMIRT
                     RLRGNPVPIQLPIGAEDKFEGVVDLVRMKAIYWDMETQGTKFEYGEIPERLKAQAEEY
                     HAKMVEAAAEGNEELLNKYLEEGTLTDEEIKHGLRERSLRGEIVLCMCGTAFKNKGVQ
                     ALLDAVIEYMPSPTEVADVKGLNEAGEPDTRPSSDEAPFSALAFKILNDPFVGNLTFF
                     RVYSGVLNSGDTVYVPGKGRKERIGRLLQMHASERTEIKEVRAGDIAAAVGLKDVVTG
                     DTLCDIEKVITLEKMVFPEPVISVAIEPKTKADQEKMGLALQRLAKEDPSFRVSTDQE
                     SGQTIIAGMGELHLEIIVDRMKREFKVEANVGKPQVAYRETIRGSVESEGKFVRQSGG
                     RGQYGHVWLKIEPQPEGKGYEFVNGIVGGTVPREFIPAVDKGIREAVETGVIAGYPVV
                     DVKITLFDGSYHDVDSNEMAFKIAGSMGFKEGFRKAKPVLLEPIMKVEVVTPEEYSGD
                     VIGDLNRRRGQIQGMEDGPAGKVISAEVPLSEMFGYATNVRSMSQGRATFTMEFAKYL
                     EVPPNIAESLIKK"
     gene            complement(2196..2675)
                     /gene="rpsG"
                     /locus_tag="PKN91_11345"
     CDS             complement(2196..2675)
                     /gene="rpsG"
                     /locus_tag="PKN91_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003369032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNS28703.1"
                     /translation="MSRRAQAPHREILPDPKFHSDMLAKFMNVVMESGKKSAAERIIY
                     GAIDRIGEKTGKQGADALAVLSQALDNVKPVVEVKSRRVGGATYQVPVEVRATRRQTL
                     AMRWVIEAARGRSEKSMSARLAHELMEAAENRGAAVRKREDTHRMAEANKAFAHYRW"
     gene            complement(2691..3065)
                     /gene="rpsL"
                     /locus_tag="PKN91_11350"
     CDS             complement(2691..3065)
                     /gene="rpsL"
                     /locus_tag="PKN91_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNS28704.1"
                     /translation="MATTGQLIRQPRRTRKEKTKVPALGAAPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLVNGYEVSSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGTL
                     DCAGVNERKQSRSKYGAKRPKK"
     gene            3301..4011
                     /locus_tag="PKN91_11355"
     CDS             3301..4011
                     /locus_tag="PKN91_11355"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNS28705.1"
                     /translation="MSDAKAASLPLTGLVIAIGLAAGGWFIGHGLLEARSADRYVTVR
                     GLAEREVKADLVIWPLRNAVTANDLVTLQRLSDESARKVRAFLDKRFEGDDITVSAPQ
                     IQDRDAQGMTNRGNVQRYVAEVVVTLRTNRIDEVRRANEQLGELVSEGVALMQNWESR
                     TQFSYTGLEQVKPEMIAEATRDARKAAEQFAEDSGSKVGSIRNAQQGYFSIDDRDAFS
                     PEYKRIRVVTTVQFFLED"
     gene            complement(4038..4403)
                     /locus_tag="PKN91_11360"
     CDS             complement(4038..4403)
                     /locus_tag="PKN91_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28706.1"
                     /translation="MRRLAAVLPFAWLAIGCASQPRALALDHVEATRWNGGTLEVSSA
                     RGIGGLTVEPAPARPLRICFGYDFSRPFGRLEGLEVVAVARGGQRSALGTTVDGACAT
                     VDAAPGAASLRVQFVDFYR"
     gene            complement(4432..>5066)
                     /locus_tag="PKN91_11365"
     CDS             complement(4432..>5066)
                     /locus_tag="PKN91_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28707.1"
                     /translation="RQLNVFEGEQVERGEVIADGEPNPHDILRLQGIEALANYLTREI
                     QDVYRLQGVKINDKHIEVIVRQMLRKADVASPGDTPLLKGEQLDRARALEINDRVALE
                     GKQQATLSPVLLGITKASLATESFISAASFQETTRVLTEAAVRGLKDDLRGLKENVIV
                     GRLIPAGTGFAAHAHRRRKSTAERRSFMDVGGGVADIDEPTVTEEESAAG"
BASE COUNT          789 a         1708 c         1630 g          939 t
ORIGIN      
        1 cctatcgtcc ccacgttcac gtgcggcttg ctgcgttcga atttctcttt ggacatggac
       61 ttttcctcaa tcccggattt atttcttgat caacgactcg gcgatgttcg gcggcacttc
      121 gaggtacttg gcgaactcca tcgtgaacgt cgcgcggccc tggctcatcg agcgtacgtt
      181 ggtcgcgtag ccgaacatct ccgacagcgg cacctcggcc gagatcacct tgccggcagg
      241 cccgtcttcc atgccctgga tctggccgcg gcggcgattc aggtcgccga tcacgtcacc
      301 ggaatattcc tccggcgtca cgacctcgac cttcatgatc ggctcgagca gcaccggctt
      361 cgccttgcgg aagccctcct tgaagcccat cgagccggcg atcttgaacg ccatctcgtt
      421 cgagtcgacg tcgtggtacg aaccgtcgaa cagcgtgatc ttcacgtcga cgaccggata
      481 gccggcaatg acgccggtct cgacggcctc acggatgccc ttgtcgaccg ccgggatgaa
      541 ctcgcgcggc acggtgccgc cgacgatgcc gttgacgaat tcgtagccct tgccttccgg
      601 ctgcggctcg atcttgagcc acacgtggcc gtactggccg cggccgcccg actggcgcac
      661 gaacttgccc tcggactcga ccgaaccgcg gatcgtctcg cggtaggcga cctgcggctt
      721 gccgacgttc gcctcgacct tgaactcgcg cttcatgcga tcgacgatga tctcgaggtg
      781 cagctcgccc atgccggcga tgatcgtctg ccccgattcc tggtccgtgc tgacgcggaa
      841 cgacgggtct tccttggcga ggcgctgcag cgcgaggccc atcttctcct ggtcggcctt
      901 ggtcttcggc tcgatcgcga ccgagatcac gggctcgggg aacaccatct tctcgagagt
      961 gatcaccttc tcgatgtcgc acagcgtatc gccggtgacg acgtccttca gacccacggc
     1021 cgcggcgatg tcgccggcgc gcacttcctt gatctcggtg cgctcgctcg catgcatctg
     1081 cagcaggcgg ccgatacgct ccttcctgcc cttgcccggc acgtacacgg tatcgccgga
     1141 attgagcacg ccggaataga cgcgaaagaa ggtcaggttg ccgacgaacg ggtcgttcag
     1201 gatcttgaag gcgagcgccg aaaacggcgc ctcgtcgctc gacgggcgcg tatcgggctc
     1261 gccggcctcg ttcaggccct tgacgtccgc gacctcggtg ggcgagggca tgtattcgat
     1321 gaccgcgtcg agcagcgcct gcacgccctt gttcttgaaa gcggtgccgc acatgcacag
     1381 cacgatctcg ccgcgcagcg agcgctcgcg cagaccgtgc ttgatctcct cgtcggtcag
     1441 cgtgccttcc tcgaggtact tgttgagcag ctcctcgttg ccttcggcag cagcctcgac
     1501 catcttcgcg tggtattcct cggcctgcgc cttgagacgc tcgggaattt cgccgtactc
     1561 gaacttcgtg ccctgggttt ccatgtccca gtagatcgcc ttcatgcgca cgaggtcgac
     1621 gacgccctcg aacttgtcct cggcgccgat cggcagctgg atcggcaccg gattgccgcg
     1681 caggcgcgtg cggatcatgt cgacgacgcg caggaaattg gcgcccgcgc gatccatctt
     1741 gttgacgaat gcgatgcgcg gcacgttgta gcggttcatc tggcgccaca cggtctcgga
     1801 ctgcggctgc acgccggcga ccgagtcgaa cagtgccacc gcgctgtcga gcacacgcag
     1861 gctgcgctcg acttcgatcg taaagtcgac gtggcccggc gtgtcgatga tgttgatgcg
     1921 atgctcgggg aaggacttgt ccatgccctt ccagaaacag gtcgtcgccg acgaggtgat
     1981 cgtgatgccg cgctcctgct cctgctccat gtagtccatg atggcggcgc cgtcatgcac
     2041 ctcgccgagc ttgtgcgata cgccggtgta gaacaggatg cgctcggtcg tcgtcgtctt
     2101 gccggcgtcg atatgcgccg aaatgccgat gttgcgatag cgctcgatgg gagtggtgcg
     2161 gggcacttag gaatccttga cagtaaacgg ggccgttacc agcgatagtg cgcgaaggcc
     2221 ttgttggcct cggccattcg gtgcgtgtct tcacgcttgc gtacggccgc gccgcggttc
     2281 tcggcggctt ccatcagctc gtgcgcgagg cgggccgaca tcgacttctc gctgcgaccg
     2341 cgcgcggcct cgatcaccca gcgcatcgcc agggtctggc gccgcgtggc gcgcacttcg
     2401 accggcacct ggtaggtggc gccgccgacg cggcgcgact tcacctcgac caccggcttc
     2461 acgttgtcga gcgcctgcga cagcaccgcc agggcgtccg caccctgctt gccggtcttc
     2521 tcgccgatgc ggtcgatcgc accatagatg atgcgttcgg cagcggactt cttgccgctt
     2581 tccatgacga cgttcatgaa cttcgccagc atgtcgctgt ggaatttcgg atcgggaagg
     2641 atctcgcgat ggggcgcctg ggctcgacgt gacatgatgc tttacctggt ttacttcttg
     2701 gggcgcttcg cgccgtactt ggagcggctc tgcttgcgct cgttgacgcc agcgcagtcg
     2761 agggtgccgc gcacggtgtg atagcgcacg cccggcaggt ccttgacgcg accgccacgg
     2821 atcagaacga ccgagtgctc ctggagattg tgaccctcgc cgccgatata cgagctgacc
     2881 tcgtagccgt tgacgagccg cacgcggcac actttgcgaa gcgcggagtt cggcttcttc
     2941 ggcgtggtgg tgtacacacg ggtgcagacg ccgcgcttct gcggtgcggc gccgagcgcc
     3001 ggcaccttgg ttttttcctt gcgggtccgg cgtggttgcc ggatcaactg acccgtagtg
     3061 gccatgaaat ctccaggaat gtgaagcaac accgggccgc tccacgacga aggggcccgg
     3121 tgttgggtat gtcgctaatt tccccgcccg gctgcattcg tgcccgggcg aaaaggcggg
     3181 ggattctagg tagccatcag gatgctgtca atgcatgctg catagcacaa tcccgcgagg
     3241 gcgcactggc gagccgatcc cgggcccacg ttgcgcgccg ccttgtagac tacatgtccc
     3301 atgagcgacg caaaagcggc ttccctgccc ctcacgggcc tcgtcatcgc gatcggcctc
     3361 gcggccggcg gctggttcat cggccacggc ctgctcgagg cccgctccgc ggaccgctat
     3421 gtcaccgtga gaggactcgc ggagcgcgag gtcaaggccg atctcgtgat ctggccactg
     3481 cgcaatgccg tcaccgccaa cgatctcgtg acactgcagc gcctgtccga cgagagcgcg
     3541 cgcaaggtgc gcgccttcct cgacaagcgc ttcgagggcg acgacatcac cgtgtcggcg
     3601 ccgcagatcc aggaccgcga cgcgcaaggc atgacgaatc gcggcaacgt gcagcgctac
     3661 gtcgccgagg tggtggtcac gctgcgcacg aaccggatcg acgaggtgcg ccgcgccaac
     3721 gaacagctcg gcgagctcgt cagcgagggc gtggcgctga tgcagaactg ggagagccgc
     3781 acgcagttct cctacaccgg cctcgagcag gtgaagccgg agatgatcgc cgaggcgacc
     3841 cgtgacgcgc gcaaggcggc cgagcagttc gccgaggact cgggcagcaa ggtcggctcg
     3901 atccgcaacg cccagcaggg ttatttctcg atcgatgatc gcgatgcgtt ttcgcccgag
     3961 tacaagcgga tccgcgtcgt gacgacggtg cagttcttcc tggaggactg agcgaggggc
     4021 cgcttgccgg gctgcgttca gcggtagaaa tccacgaact ggacgcgcag cgatgccgcg
     4081 cccggcgcgg cgtccaccgt ggcgcaggcg ccatccacgg tagtcccgag tgccgagcgc
     4141 tgcccgcccc gggccaccgc gacgacctcg aggccctcga ggcggccgaa gggacgcgag
     4201 aagtcgtaac cgaagcagat cctgagcggc ctggccggcg ccggttccac ggtgaggcca
     4261 ccgatgcccc gcgcactgct cacctcgagg gtgccaccgt tccagcgagt cgcctcgaca
     4321 tgatcgagcg cgagcgcgcg cggttgcgat gcgcagccga tggcgagcca ggcgaacggc
     4381 agcaccgcag ccaaccgcct catgccaccg tcccgcctgc gcccgggcgc ctcaacccgc
     4441 ggcgctctcc tcttcggtga ccgtcggctc gtcgatgtcc gccacgccgc cgcccacgtc
     4501 catgaagctg cggcgctcgg cggtcgactt gcgacgacgg tgggcgtggg ccgcgaagcc
     4561 cgtgcccgcc gggatcagcc ggccgacgat gacgttctcc ttgagcccgc gcagatcgtc
     4621 cttcaggccg cgcaccgcgg cctcggtcag cacgcgcgta gtctcctgga acgatgccgc
     4681 ggagatgaac gactccgtcg cgagcgacgc cttggtgatg ccgagcagca ccggcgagag
     4741 cgtcgcctgt tgcttgcctt ccagcgcgac gcgatcgttg atctcgagcg cgcgggcgcg
     4801 atcgagctgc tcgcccttga gcagcggcgt atcccccggc gaagccacgt cggccttgcg
     4861 cagcatctgc cgcacgatca cctcgatgtg cttgtcgttg atcttcacgc cctgcaggcg
     4921 gtagacgtcc tggatctcgc gcgtcaggta gttggcgagc gcctcgatgc cctgcaggcg
     4981 caggatatcg tgcggattcg gttcaccgtc cgcgatcact tcgccgcgct cgacctgctc
     5041 gccctcgaag acgttcagct ggcgcc
//
LOCUS       DAOQVY010000150         4798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_19687_length_4798_cov_5.877504, whole genome shotgun sequence.
ACCESSION   DAOQVY010000150 DAOQVY010000000
VERSION     DAOQVY010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19687_length_4798_cov_5.877504"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PKN91_11370"
     CDS             <1..373
                     /locus_tag="PKN91_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNS28708.1"
                     /translation="EDTRIGDDVKLDNQIQIGHNVRIGAHTAMAACVGVSGSATIGKR
                     CQIGGAVGIVGHLSICDDVAVTGLTMVSHSITEPGVYSSGLPAVPAGEWRRAVARLHR
                     LDRMAGRLAALERQLDKDQHD"
     gene            366..833
                     /gene="fabZ"
                     /locus_tag="PKN91_11375"
     CDS             366..833
                     /gene="fabZ"
                     /locus_tag="PKN91_11375"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003377815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNS28709.1"
                     /translation="MTDPVFLDIDGILRKLPHRYPFLLVDRVLECEPGKSIRALKNVT
                     YNEPFFPGHFPHRPVMPGVIMLEALAQAAGILAFVTADFIPDENRLFYFAGIDEARFR
                     RPVEPGDQLVLTAQFERAFKGIWRFSTRGMIDGGEVCSATIMIAPEAKKAAKS"
     gene            830..1600
                     /gene="lpxA"
                     /locus_tag="PKN91_11380"
     CDS             830..1600
                     /gene="lpxA"
                     /locus_tag="PKN91_11380"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR01852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNS28710.1"
                     /translation="MIDSRALISPQAKLAPDVVVGPFAVIGPGVEIGPGCTVGAHAMI
                     ERDTRLGARNHVHQFASLGSAPQDKSYKGEPTRLEIGDDNVFREFCTVNRGTSKDQGV
                     TRIGSGNLFMSYSHVAHDCVVGDKVVLANLATLGGHVQVGDWVIMAGFSGVHQFCRIG
                     AHAFIANNAAVTRDVPPYVMAVGQPAVPHSVNSEGLKRRGFSPVQIRNIRNAFRTLYR
                     EDRKLEDAVGALRAVAREQPEIAAFVTFIDAATRSLVR"
     gene            1609..2751
                     /gene="lpxB"
                     /locus_tag="PKN91_11385"
     CDS             1609..2751
                     /gene="lpxB"
                     /locus_tag="PKN91_11385"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNS28711.1"
                     /translation="MALRVGIVAGEASGDTLGAAFITALRERDPGVQVFGVAGPKMIA
                     AGCEAWESSESLAVMGVTEVLRHLPRLWRLRRRLAQRFLAAPPDVFVGIDAPEFNLGL
                     ERRLKGAMPTVQYVSPQVWAWRQGRVRGIGRSCDLVLCLLPFETRFYAEHDVRAVFVG
                     HPLADRIPLAVDRDAARAALGVAREATVVAVLPGSRVGEVTRLGADFAAACAMLARQR
                     PDTLFLAPMANAAVRELFDAQWREHAPGICARLLDGQADGAIIAADVVLVASGTATLE
                     TTLCKRPMVVAYRLGAITALLLRTLGLVKVAHFSQPNLLAGEELVPEFFQEAVTPAAL
                     AGALAALLDDPARAALLARRFLAIHESLRVDGARRAADAVLELVRR"
     gene            2748..3335
                     /locus_tag="PKN91_11390"
     CDS             2748..3335
                     /locus_tag="PKN91_11390"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNS28712.1"
                     /translation="MSERVRIAGVDEAGRGPLAGPVVAAAVILDPLRAVHGLADSKKL
                     EPAERERIATRIRARSLAWAVAWADPCEIDQLNILEATLLAMRRALLGLPVRPTGVLV
                     DGNRLPRLEDLGLAAAARAVVRGDQSEPAISAASILAKTWRDALMCRLNERYPGFGLD
                     GHKGYPTPSHFAALQRSGPSPIHRLSFAPCQAGRA"
     gene            3332..>4798
                     /gene="dnaE"
                     /locus_tag="PKN91_11395"
     CDS             3332..>4798
                     /gene="dnaE"
                     /locus_tag="PKN91_11395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNS28713.1"
                     /translation="MSAVAAGAPFVHLRVHTEYSLVDSVVRVPALVTAAAAAGMPAVA
                     VTDECNLFAMVKFYRAALAAGVKPVVGADLLVRESAERAQPARLTLLCQNARGYGNIT
                     RLVSRAYREGRRQGVPLLEREWLDRDACDGLIALSGATQGDIGRAFAAGREAEAALCL
                     DQWLALFGDRFYIELQRVGRTDEEAYIAAAAALAARRGVPVVATNDVRFLSPDDFESH
                     EARVCIQDGTLLTDPGRARRYTPQQSLRSPAEMARVFQDIPEALANSVEIARRCSLPL
                     KLGEARLPEYPVPAGQTVAGHLREESARGLEGHLALRGSDPEEAGRYRARLATELDVI
                     CQMGFAGYFLIVADFIRWARGHGVPVGPGRGSGAGSVVAWALGITDIDPLRYDLLFER
                     FLNPERVSMPDFDVDFCMDGRDRVIDYVAERYGRERVSQIITYGTMAAKAVVRDVGRV
                     LGLSYGQVDRIAKLIPFELGITLDDALKKEPELRRLYEN"
BASE COUNT          672 a         1656 c         1698 g          772 t
ORIGIN      
        1 cgaggacacc cggatcggcg acgatgtcaa gctcgacaac cagatccaga tcgggcacaa
       61 cgtgcgcatc ggtgcgcaca ccgccatggc ggcatgtgtc ggcgtgtcgg gcagcgcaac
      121 gatcggcaag cgctgccaga tcggcggtgc ggtgggcatc gtcgggcacc tgtcgatctg
      181 tgacgacgtt gcggtaaccg ggctcacgat ggtgagtcat tcgatcaccg agccgggggt
      241 ctattcgagc ggtctgccgg cggtcccggc cggcgagtgg cggcgtgccg tggcgcgcct
      301 gcaccggctc gatcgcatgg ccggacggct ggcagcgctc gagaggcagc tggacaagga
      361 tcaacatgac tgaccccgtg ttcctggaca tcgacggcat cctgcggaag ctcccgcatc
      421 gctacccgtt cctgctggtc gatcgcgtcc tcgagtgcga gcccggcaaa tcgatccgcg
      481 cgctgaagaa cgttacctac aacgagccgt tctttcccgg ccacttcccg caccgcccgg
      541 tcatgccggg cgtgatcatg ctcgaggcgc tcgcgcaggc cgcgggcatc cttgcttttg
      601 tcacggccga cttcattccg gacgagaaca ggctcttcta ttttgccggc atcgacgagg
      661 cgcgcttccg ccggccggtc gagccgggcg accagctcgt gctgaccgcg cagttcgagc
      721 gcgcgttcaa gggcatctgg cgcttctcca cgaggggaat gatcgatggc ggggaggtct
      781 gctcggccac gataatgatt gcacccgaag cgaagaaagc agcgaagtca tgatcgattc
      841 gcgagcactg atctcgccgc aggcgaagct cgcaccggac gtggtcgtgg ggccgttcgc
      901 ggtgatcggc ccgggcgtcg agatcggccc gggctgcacc gtgggcgcgc acgccatgat
      961 cgaacgcgac acgcgcctcg gtgcgcgcaa ccacgtgcat cagttcgcgt cgctcggcag
     1021 cgccccgcag gacaagagct acaagggcga gccgacccgg ctcgagatcg gcgacgacaa
     1081 cgtgttccgc gagttctgca cggtgaatcg cggcacgagc aaggaccagg gcgtcacgcg
     1141 cataggcagc ggcaatctgt tcatgtccta ttcgcacgtc gcgcacgact gcgtcgtcgg
     1201 cgacaaggtg gtgctcgcga acctcgcgac gctcggcggt cacgtgcagg ttggcgactg
     1261 ggtgatcatg gcgggcttct ccggcgtgca ccagttctgc cgcatcggcg cgcacgcgtt
     1321 catcgcgaac aacgcggcgg tcacgcgcga cgtgccgccg tacgtgatgg ccgtcggcca
     1381 gccggccgtg ccgcactcgg tcaattccga gggactcaag cggcgcggct tctcgccggt
     1441 gcagatccgc aatatcagga atgcgtttcg caccctctac agggaggacc gcaagctcga
     1501 ggacgccgtg ggcgcattgc gcgcggtggc cagggagcag cccgagatcg cggccttcgt
     1561 caccttcatc gacgcggcga cccgcagcct cgtccgctga ccaaccgcat ggcattgcgc
     1621 gtcggaatcg tggccggcga ggcctcgggc gacacgctcg gtgccgcgtt catcaccgcg
     1681 ctgcgcgagc gcgatcccgg ggtgcaggtc ttcggcgtcg cgggtccgaa gatgattgcc
     1741 gcgggctgcg aggcgtggga atcgtccgag tcgctcgccg tcatgggcgt caccgaggta
     1801 ctgcggcacc tgccgcggct gtggcggctg cgccggcgcc tcgcgcagcg gttcctcgcc
     1861 gcgccccccg atgtattcgt cggtatcgat gcgccggagt tcaacctcgg gctcgaacgg
     1921 cgcctgaagg gcgcgatgcc caccgtgcag tacgtgagcc cgcaggtgtg ggcatggcgg
     1981 cagggtcgcg tgcgcggcat cggccgatcc tgcgatctcg tgctgtgcct gctgccgttc
     2041 gagacacgct tctatgcgga gcacgacgtg cgcgccgtgt ttgtcggcca cccgcttgcg
     2101 gatcgcattc cactcgccgt cgatcgcgat gccgcacgcg cagccctggg cgttgcgcgc
     2161 gaggcgaccg tggtcgcggt gctgccgggc agtcgggtcg gggaagtcac ccgcctcgga
     2221 gccgatttcg ctgcggcctg cgcgatgctc gcgcggcagc ggcccgacac cctgttcctc
     2281 gcgccgatgg cgaatgccgc ggtccgcgag ctgttcgatg cgcagtggcg cgagcacgcc
     2341 cccggaatct gcgccaggct cttggatggt caggcagacg gggccatcat tgctgctgac
     2401 gtcgtgctcg tggcgtccgg gaccgcgacg ctcgagacga ccttgtgcaa gcggccgatg
     2461 gtcgtggcgt accggctggg ggccatcacg gcgctgctgc tgcgtaccct ggggctcgtg
     2521 aaggtggcgc atttctccca acccaatctg ctcgccgggg aggaactcgt accggagttc
     2581 ttccaggagg ccgtgacgcc ggcggcgctc gcgggcgcgc tcgccgcgct gctcgacgac
     2641 cccgcgcggg cggcgctgct cgcgcgccgc ttcctcgcga tccacgagtc gctgcgtgtc
     2701 gacggcgcgc gccgcgctgc cgacgccgta ctggagctcg tgcgtcggtg agcgagcgag
     2761 tgcgcatcgc gggcgtcgat gaagccgggc gcggcccgct ggcaggaccg gtggtcgcag
     2821 ccgccgtgat cctcgatccg ctccgggcgg tgcatggcct tgccgattcg aagaagctcg
     2881 aaccggccga acgcgagcgg atcgcgaccc gcatccgcgc ccgctcgctc gcgtgggcgg
     2941 tcgcgtgggc cgatccatgc gagatcgacc agctgaatat cctggaagcc acgcttcttg
     3001 cgatgcgccg cgcgctgctc ggcctgccgg tgcgcccgac cggcgtactc gtcgacggca
     3061 accgcctgcc acgcctcgaa gacctcggac tcgcagccgc cgcgcgcgcg gtggtgcgcg
     3121 gcgaccagtc ggaacccgcg atcagcgccg cgtccatcct tgccaagacc tggcgcgacg
     3181 cgctgatgtg ccgcctgaac gagcgctatc cgggcttcgg cctcgacggc cacaagggct
     3241 atccgactcc ctcgcatttc gcggcgctgc agcgaagtgg gccgtcgccg atccatcggc
     3301 tctccttcgc cccctgccag gcaggtcgcg cgtgagcgcc gtcgccgccg gcgcgccttt
     3361 cgtgcacctg cgggtgcaca ccgagtactc gctcgtcgac agcgtggtgc gcgtgcccgc
     3421 gctcgtcacg gcggccgccg cggccggcat gcccgccgtg gccgtgaccg acgagtgcaa
     3481 cctgttcgcg atggtgaagt tctatcgcgc ggcgctggcg gcgggcgtga agcccgtggt
     3541 cggcgccgac ctgctggtgc gcgagagtgc cgagcgtgcc cagccggcgc ggctcacgct
     3601 gctgtgccag aacgcgcgcg gctacggcaa catcacacgc ctcgtgagcc gcgcctatcg
     3661 cgagggacgt cgccagggcg tgccgctgct cgagcgtgag tggctcgatc gcgacgcctg
     3721 cgacgggctg atcgccctct cgggcgccac ccagggcgac atcggccgtg cgtttgccgc
     3781 cggtcgcgag gccgaggccg cgctgtgcct cgaccaatgg ctcgcgctgt tcggtgatcg
     3841 cttctacatc gagctgcagc gtgtcgggcg caccgacgaa gaggcttaca tcgcggccgc
     3901 cgcggcgctg gccgcgcgcc gtggcgtgcc ggtggtcgcc accaacgacg ttcgtttcct
     3961 gtcaccggac gatttcgagt cgcacgaggc acgggtctgc atccaggacg gcacgctgct
     4021 caccgatccg gggcgcgccc gtcgctacac gccgcagcaa tcgctgcgct cgcccgccga
     4081 gatggcgcgc gtgttccagg acataccgga ggcgctggcg aacagcgtcg agatcgcacg
     4141 tcgctgcagc ctgccgctga agctcggcga ggcccgcctg cccgagtatc cggtgccggc
     4201 ggggcagacc gtcgccgggc acctgcggga ggaatcggcg cgcggcctcg aggggcatct
     4261 ggcgctgcgg gggtccgatc cggaggaggc cggtcgctat cgcgcgcggc tcgcgaccga
     4321 gctcgacgtg atctgccaga tgggattcgc cggctacttc ctgatcgtcg ccgacttcat
     4381 ccgctgggcg cgcggccatg gtgttccggt ggggccgggc cgcggctccg gcgccggctc
     4441 ggtcgtcgcc tgggcgctcg gcatcaccga catcgatccg ctgcgctacg acctgctgtt
     4501 cgaacgcttc ctgaatcccg aacgcgtgtc gatgcccgac ttcgacgtcg acttctgcat
     4561 ggacggtcgc gaccgcgtga tcgactacgt ggcggagcgg tacgggcgcg agcgggtttc
     4621 acagatcatc acctacggca ccatggcggc aaaggcggtg gtgcgcgacg tcgggcgcgt
     4681 gctcggcctg agctacggcc aggtcgaccg gatcgccaag ctgatcccgt tcgagctcgg
     4741 catcacgctc gatgacgcgc tcaagaagga gccggagctg cggcggctct acgagaac
//
LOCUS       DAOQVY010000151         4794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_19714_length_4794_cov_4.111627, whole genome shotgun sequence.
ACCESSION   DAOQVY010000151 DAOQVY010000000
VERSION     DAOQVY010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19714_length_4794_cov_4.111627"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="PKN91_11400"
     CDS             complement(<1..1458)
                     /locus_tag="PKN91_11400"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS28714.1"
                     /translation="MTREETLELHRQGRLEEAEQGYRELLAANPNDGEALRLLGVVRR
                     ARGDLEEAATLIRRAHELAPEQPRLLLMLGAIQFQLGNIERSRTAYEQALALDPNSAG
                     AHSTLGHIAMLNADPTLAEQYFRTALRVEEDAQAYAGLGMIALDREDIDTAMKYLSRA
                     ADLSPNDASVAFALGRGFVKRGMIAFAEQSFRRALELQPGQAHASNALGQLLLREHRA
                     AEAEPFMRSLLNRRNFELAGELGLGDVYRALGRHEDAVAQYRRALERAPHHEAGFEAL
                     LWCLRQLGRAREALALLDERIERFPLQTRWRAERAMLNTQLGRDVEAVDEWQVLHDRD
                     PSNDQVVLELARQRERMGDFDAANELAERLAVRYPDEQEATLIRARARLRSGNDEAAK
                     ALLDLIAGRTTRDDVARHSLNLLGRLHDRAGRYDEAVKHFRASQHGLTGMLPRLETLP
                     DASEYARVIAQAQGEKWAHAPILLVGTPGSGVERIA"
     gene            1603..3078
                     /locus_tag="PKN91_11405"
     CDS             1603..3078
                     /locus_tag="PKN91_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS28715.1"
                     /translation="MGANQDLATLLRARTPLLVVETPEEKRVIESFRHAIAQSLRPLY
                     QWSITDGLRRLDMDDDEIEGLPDATLTLTTIKQRPEPAVFLLLDFQPYLRYTMTLRLL
                     REIVLRQDAAEHTLVLVGARIELPDDLAPMATRFELALPDADALAKIVREEAFNYSRE
                     HGRRVEVDPEAARTVVRNLRGLTVADARRIVRKLIYADGALGPADLPELAKAKFALLD
                     RDNLLHFEYETARFTDVAGLGRLKRWIAQREPAFVGRKMPVRLDPPKGILLLGVQGCG
                     KSLAAKAVAGGFGVPLLRLDIGALYNKYHGETERNLRGALKSAELLAPCVLWLDEIEK
                     ALATAGNDDGVSRRVLGYLLTWMAERKTPVFLVATANDVQALPPELLRKGRFDEIFFV
                     DLPDEATRVELVRIHLAKRDLDPAQFDLAEVARASAGFSGAEIEQLVVAGLYAAAAAN
                     RPLDTAHLIDEARATRPLSVMMASQVEALRAWARERTVPAD"
     gene            3189..3575
                     /locus_tag="PKN91_11410"
     CDS             3189..3575
                     /locus_tag="PKN91_11410"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HNS28716.1"
                     /translation="MNHIDLVALLAVVQLVFFAILVGRARGRYGVKAPAVSGHEMFER
                     AYRVQMNTLELMVMFLPALYIAAKYWPAQYIAGIGAVYLIGRLIYWRSYTAAPARRGL
                     GFALSMIPVLALLIAGLVGIIRNAAG"
     gene            complement(3653..4672)
                     /locus_tag="PKN91_11415"
     CDS             complement(3653..4672)
                     /locus_tag="PKN91_11415"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNS28717.1"
                     /translation="MNAADYRPPRLLRNPHVQSVLASSGVRRLLHARRRALLEDGAVA
                     HVLDCGDGVRLQGFHTAQRAQAQARGLVVLLHGWEGSVTSSYLLHTGARLLAEGFDVF
                     RLNFRDHGDTHHLNRGLFHSCRIDEVVGAVGAISRMFPRWPLAVAGFSLGGNFALRVA
                     LRAPGAGIDLSYALAVCPAINPRGVLHAIESAPWFYEHYFLRKWRRSLLLKQRAFPDA
                     DLFDAADLRGNLREMTRMLVQRHTDFGTLEAYLDGYSIAGDRLAALAVPATILTAADD
                     PIIPVEDFHVLVLPPIAELDIAPGGGHCGFIRDLSLRSWTEDYIAERMAARLSASRRA
                     DDPGT"
BASE COUNT          749 a         1638 c         1655 g          752 t
ORIGIN      
        1 ggcgatgcgt tcgaccccgc tgccgggcgt gccgaccagc agaatcggtg catgcgccca
       61 cttctcgccc tgcgcctggg cgatgacgcg cgcgtactcg cttgcatccg gcagcgtttc
      121 caggcgcggc aacatgccgg tcaggccatg ttgtgaggca cggaagtgct tcacggcctc
      181 gtcgtaacgg cctgcacgat cgtgcaggcg gccgagcagg ttgagcgagt ggcgggcgac
      241 gtcatcgcgc gtggtgcgac ccgcgatcag gtcgagcagg gccttggcgg cctcgtcgtt
      301 gccgctgcga aggcgcgcgc gcgcacgaat cagggtcgct tcctgttcgt ccggatagcg
      361 cacggcgaga cgctcggcga gctcgttggc cgcgtcgaaa tcacccatgc gttcccgctg
      421 cctggccagt tcgagcacga cctggtcgtt ggacggatca cggtcatgca gcacctgcca
      481 ttcatcgacg gcctcgacgt cgcggccgag ctgggtgttc agcatcgcgc gctcggcacg
      541 ccagcgggtt tgcagcggaa accgctcgat gcgctcatcg agcaaggcga gcgcttcccg
      601 cgcgcggccg agctggcgca agcaccacag caaggcctcg aagccggctt cgtgatgtgg
      661 tgcacgctcg agcgcgcggc ggtactgggc gaccgcgtcc tcgtggcggc caagcgctcg
      721 gtagacgtca ccgaggccaa gttcgccagc cagctcgaag ttgcgtctgt tgagcaggga
      781 acgcatgaac ggctcggcct cggccgcgcg gtgttcgcgc agcaggagct gcccgagcgc
      841 attgctcgcg tgtgcctggc cgggctgcag ctccagcgca cggcggaacg actgctcggc
      901 gaacgcgatc atgccccgct tgacgaaacc gcgaccgagc gcgaaagcga ccgaggcgtc
      961 gttcggcgaa aggtcggccg cgcgcgacag gtatttcatc gccgtatcga tgtcctcgcg
     1021 atcgagcgcg atcatgccga gaccggcata ggcctgcgca tcctcctcga cacgcagcgc
     1081 ggtgcggaag tactgctcgg cgagggtcgg atcagcgttg agcatggcga tatggcccag
     1141 cgtggaatgc gcgccggcgc tgttcggatc gagcgcgagc gcctgttcgt aggcagtgcg
     1201 cgaccgttcg atgttgccga gctggaactg tatggcgcca agcatgagca gcaggcgcgg
     1261 ttgttcgggc gcgagctcat gggcgcgccg gatcagcgtc gccgcctcct cgaggtcgcc
     1321 gcgcgcgcgg cgcacgactc ccagcagacg caaggcctcg ccgtcattcg gattcgcggc
     1381 gagcagctcg cggtagccct gctcggcctc ttcaaggcgg ccctggcggt gcagttcgag
     1441 ggtttcttct ctggtcatgg tcattcggta tgggagggcc ggggccgcac ggggaccgta
     1501 cattatgccg cacgacggcg attcggcccg caggacgctg cgtgcctccg tgctaggctc
     1561 agcggcccgc acctcgaacc gaatccgccg gaaccgccac cgatgggagc caaccaggac
     1621 ctcgccaccc tgttgcgcgc gcgcacgcca ttgctggtcg tcgaaacgcc cgaggaaaaa
     1681 cgcgtcatcg aaagtttccg gcacgccatc gcgcagagcc tgcgcccgct gtaccagtgg
     1741 agcatcaccg atggcctgcg tcggctggac atggacgacg acgagatcga aggcctgccc
     1801 gatgccacgc tcaccctgac cacgatcaag cagcgccccg agccagccgt gttcctgctg
     1861 ctcgatttcc agccgtacct gcgctacacg atgacgctgc gcctgctgcg cgagatcgtg
     1921 ctgcgccagg atgccgccga acataccctc gtgctggtcg gcgcgcgcat cgaactgccc
     1981 gacgaccttg caccgatggc gacgcgcttc gagctcgccc tgcccgatgc cgacgcgctg
     2041 gcgaagatcg tgcgcgagga ggcgttcaac tattcgcgcg agcacggccg gcgcgtcgaa
     2101 gtcgatcccg aggccgcgcg caccgtcgtg cgcaacctgc gcggcctcac cgtcgccgac
     2161 gcccggcgca tcgtgcgcaa gttgatctat gccgacggcg cgctcgggcc cgccgacctg
     2221 cccgaactcg ccaaggccaa gttcgccctg ctcgaccgcg acaatctgct gcacttcgag
     2281 tacgaaaccg cacgcttcac cgatgtcgcc ggcctcggcc gcctcaagcg ctggatcgcc
     2341 cagcgcgaac cggccttcgt cggccgcaag atgccggtca ggctcgaccc accgaaaggc
     2401 atcctcctgc tcggcgtgca gggctgcggc aaaagtctcg ccgccaaggc cgttgccggc
     2461 ggcttcggcg tgcccctgct gcgcctggac atcggcgcgc tgtacaacaa gtaccatggc
     2521 gaaaccgagc gcaacctgcg cggcgcgctg aagagcgccg aattgctagc gccctgcgtg
     2581 ctctggctcg acgagatcga gaaagcgctc gccaccgccg gcaacgacga tggcgtctcg
     2641 cgccgcgtgc ttggatacct gctcacctgg atggccgagc gcaagacacc ggtcttcctc
     2701 gtcgccaccg ccaacgacgt gcaggcactg ccacccgaac tgctgcgcaa gggtcgcttc
     2761 gacgagatct tcttcgtcga cctgccggac gaagcgaccc gcgtcgaact cgtccgcatc
     2821 cacctcgcca agcgcgatct cgacccggcg cagttcgacc tggccgaggt cgcccgcgcc
     2881 agcgcaggtt tctccggcgc ggaaatcgaa caactcgtcg tcgccggtct gtacgccgcc
     2941 gccgccgcca accgcccgct cgataccgcc catctcatcg acgaagcgcg cgccacgcgc
     3001 ccgctctcgg tgatgatggc cagccaggtc gaggccttgc gtgcctgggc gcgggaaagg
     3061 acggtgccgg cggattgaac tgccagggcc ggcgcggcga ttgccatcgg cgcgcccatc
     3121 cgcgcggtga cccgcgcagc cgaccctggt gcgtgtcact gcccgcatgc catgcacagg
     3181 gggaagccat gaaccacatt gatctcgtcg cgctgcttgc cgtggtgcag ctggtgttct
     3241 tcgcgatcct ggtcggccgc gcgcgcggcc gatacggcgt caaggcaccg gccgtgtccg
     3301 gacacgagat gttcgagcgt gcctaccggg tgcagatgaa caccctcgag ctgatggtga
     3361 tgttcctgcc cgcgctgtac atcgccgcga aatactggcc ggcgcagtac atcgcgggca
     3421 tcggtgccgt gtacctgatc ggccggctca tctactggcg cagctatacc gccgcaccgg
     3481 cgcgacgtgg tctgggtttc gcgctgtcca tgattccggt gcttgccctg ctcatcgccg
     3541 gcctggtcgg gatcatccgc aacgctgccg gctgagtgtt cgcacccgct tcgatcacgc
     3601 cgccggttgg ccatgcgaac ggtggatgtc cgggccgcgc ggcgcacccc ggtcaggtgc
     3661 ctggatcgtc ggctcgtcgc gacgcgctca gccgtgccgc catccgctcg gcgatgtagt
     3721 cctcggtcca gcttctcagc gagagatcgc ggatgaagcc gcagtggccg ccaccggggg
     3781 cgatgtcgag ttcggcgatg ggcggcagga ccaggacgtg gaagtcctcg accgggatga
     3841 tcgggtcgtc ggcggcggtg aggatggtgg caggcacggc gagcgcggcg aggcggtcgc
     3901 cggcgatcga gtagccgtcg aggtaggcct ccagcgtgcc gaagtcggtg tggcgctgga
     3961 ccagcatgcg ggtcatctcc cgcaggttgc cgcgcaggtc ggcggcatcg aacaggtcgg
     4021 catcgggaaa agcgcgctgc ttgagcagca gcgagcggcg ccatttgcgc aggaagtagt
     4081 gttcgtagaa ccacggcgcc gactcgatcg cgtgcagcac gccgcgcggg ttgatggccg
     4141 gacagacggc cagcgcgtag ctcaggtcga tgccggcgcc gggtgcgcgc agggcgacgc
     4201 gcagggcgaa gttgccgcct agcgagaagc cggcgacggc caggggccat cgcgggaaca
     4261 tgcgcgagat cgcaccgacc gcaccgacga cttcatcgat ccggcaggag tggaacaggc
     4321 cacggttgag atgatgtgtg tcgccgtggt cgcggaagtt gaggcggaac acgtcgaagc
     4381 cctcggcgag caggcgcgcg ccggtgtgca gcaggtaact cgaggtgacg ctgccttccc
     4441 agccgtgcag caggacgacg aggccgcgcg cctgcgcctg cgcgcgctgc gcggtgtgga
     4501 atccctgcag acgcactccg tcgccgcagt cgagcacatg tgcgacggca ccgtcctcga
     4561 gcagggcgcg tcggcgcgca tgcagcaggc ggcgtacgcc gctggaggcg agcacggatt
     4621 gcacatgcgg attgcgcagc aggcggggcg gccggtagtc ggccgcgttc actcctcgac
     4681 tccgcgacgt ttcttcgtgt cgacttggcc gagcgcggcc atgatgcgtg cgcgcgactc
     4741 ctcggccatg cgccggcgcg catcgggcgt cgattgcacc ggttcgagga agtg
//
LOCUS       DAOQVY010000152         4694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_20299_length_4694_cov_5.680966, whole genome shotgun sequence.
ACCESSION   DAOQVY010000152 DAOQVY010000000
VERSION     DAOQVY010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20299_length_4694_cov_5.680966"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..422)
                     /gene="ssrA"
                     /locus_tag="PKN91_11420"
     tmRNA           complement(70..422)
                     /gene="ssrA"
                     /locus_tag="PKN91_11420"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(461..1441)
                     /locus_tag="PKN91_11425"
     CDS             complement(461..1441)
                     /locus_tag="PKN91_11425"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNS28718.1"
                     /translation="MDKPDYAILGAGALGSVLGAHLARAGHRVMLLARGERAAQIERE
                     GVTLRGLAAFTMPVPVLRDPSALSAARVLIVATKTPGTAQALAALRHAAFDAAFSIQN
                     GPGKNELLAEAFGRECVLGALADTSAELLADGAVLFTRNVSVTIGELPAGSSERVRRI
                     VHEIDASGVRCKDSAQVLSLEWSKFCAWTGLMAVAVTTRLPTWRFLVDPDSALVIARI
                     VRETARLAQALGIALTDESVLPVASMASGTEADAVRSITAAGEHSRSTAPGHRVSSLQ
                     DLERGRPLEVHQTLGYAVAKTRELGLELPLLETFYSLVAALDPARQPRGT"
     gene            complement(1444..1911)
                     /gene="smpB"
                     /locus_tag="PKN91_11430"
     CDS             complement(1444..1911)
                     /gene="smpB"
                     /locus_tag="PKN91_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNS28719.1"
                     /translation="MAKEKNSDRPIAENRKARFDYFIEDRYVAGLALEGWEVKALRAG
                     RANLTEAYVYLRGGEAFLIGAHVTPLNTTSTHVHADPTRTRKLLLNRRELDQLVGAVE
                     RKGYTIVPLDLHWEKGRAKLSIGLAKGKKQHDKRATEKDRDWQRDRQRVLKRR"
     gene            1935..2381
                     /locus_tag="PKN91_11435"
     CDS             1935..2381
                     /locus_tag="PKN91_11435"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HNS28720.1"
                     /translation="MRERQVTRSALVAMPPAAIYALINDIESYPQFVPWCRTAVVESE
                     SDTEIVATLGVRRGPLQTEFTTRNILDPVRSITMEHLRGPFKRLHGAWTLVPIGDAGT
                     RIELTLRFEFANRLSASLFDSLFEETAGLLVDAFVARARSLRDAPP"
     gene            2378..2686
                     /locus_tag="PKN91_11440"
     CDS             2378..2686
                     /locus_tag="PKN91_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HNS28721.1"
                     /translation="MKRCTVAYALPHAQWLWPLELPREATVADALRAVRSAVASSGAA
                     GQLEIDWDGATVGIHGAVCARDQRFADGDRIEIYRPLGLDPRAARRARVANARRAGRR
                     "
     gene            complement(2683..3129)
                     /gene="bamE"
                     /locus_tag="PKN91_11445"
     CDS             complement(2683..3129)
                     /gene="bamE"
                     /locus_tag="PKN91_11445"
                     /inference="COORDINATES: protein motif:HMM:NF016258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNS28722.1"
                     /translation="MTRLARLAVLAFALSGLAGACVYRMTIQQGNYLDPDAVAKLQEG
                     MTRSQVRFLLGTPMVPDAFDENRWDYLYFLKQGRIKEPERRRLTVWFEDDKVARIEKV
                     NVLAPVLVPTTPAPSAPPAEDTERAPVQAPEPDQAPPQTDDANPAG"
     gene            3191..3616
                     /gene="fur"
                     /locus_tag="PKN91_11450"
     CDS             3191..3616
                     /gene="fur"
                     /locus_tag="PKN91_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HNS28723.1"
                     /translation="MEGKDLRKAGLKVTLPRLKILEILESGTPRHQSAEGIYKQLLES
                     NEDIGLATVYRVLTQFEAAGLVVRHHFENGMAVFELNEGAHHDHIVCLDCGKVEEFVE
                     TGIEERQNAVASRLGFEIRDHSLVLYGRCRRPNCPSRKP"
     gene            complement(3560..>4694)
                     /locus_tag="PKN91_11455"
     CDS             complement(3560..>4694)
                     /locus_tag="PKN91_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNS28724.1"
                     /translation="FQARELEALDMKEGELAQLTEERSRLANRGRLIEAAQGAITLLY
                     EGEEANAHASTSRALAALKAAGAHDPRLAGIAPMVEDALINLREAARELSQYAEGLDL
                     DTARLGEVEKRLAAFEELARKHRVGVAELPNRLTQLRSELSGLEHADTQLATLRKEQA
                     DALAAWRTRAGQLSSRRKAAAKSLSKEITGRMQTLGMAGGAFQIDVSPLESPEPAPHG
                     LDQVEFRVTANPGNPMRALAKVASGGELARLSLAVQVARGGREGRCMVFDEVDAGIGG
                     AVAEIVGAELRALGDRSQVLSVTHLPQVASQGHHHLRVSKLTDGRTTRTQIVALDGDA
                     RVEELARMLGGVEVTDKARAHAREMLRLSRGAVRAATAPVKNK"
BASE COUNT          711 a         1556 c         1645 g          782 t
ORIGIN      
        1 cggaaccccg cgccacgcgg ggattcgcgc aaagaaaaag ggcctggaga ctatccagac
       61 ccttgttatt ggtggaggcg gggggaatcg aacccccgtc cgcaagtcct agacttcagg
      121 atctacgtac gtagctctct ctttgtttct cgcctcacgc tacccgagga gcagggaaga
      181 cgctcagcca gcggacggta actcttagcg gaaccacccc gtcgcacgga ggcccgcgat
      241 cctatgagcg cgtcccctga ttcagtggca taggcaccag ctggtcagag gtcagccgtg
      301 attaggcggc tagagcgtag ttgtcgtcgt tggcaactaa gttttgcaca gtgattaacg
      361 agggccatgc gcctcggtac gcccctgaag gttcgcaacc cacgtcgaaa ccggtcgccc
      421 ccaaacaggt acttggtaag tatagccgat cgggccggat tcaagtcccg cgcggctgcc
      481 gcgccggatc gagcgctgca acaagcgaat agaaggtctc aagcagcggc agttcgagcc
      541 cgagttccct ggttttggcg acggcgtagc ccagcgtctg gtgcacctcg agcgggcggc
      601 cccgctcgag gtcctggagc gacgacacgc gatgaccagg cgccgtcgat cgagagtgct
      661 cgcccgcggc ggtgatgctg cggacagcgt ccgcttcggt gccgctcgcc atgctggcga
      721 ccggcagcac cgattcgtcc gtgagcgcga tgccgagcgc ctgcgcgagg cgcgccgttt
      781 cgcgcacgat gcgcgcaatc accagcgcgg agtccggatc gacgaggaat ctccatgtcg
      841 gcagccgcgt ggtcaccgcc acggccatga gtccggtcca tgcgcagaac ttcgaccact
      901 cgagcgacag cacctgcgcc gaatccttgc agcgcacgcc cgaggcgtcg atttcgtgca
      961 cgatgcgccg cacgcgctcg ctgctgcccg ccggcagttc gccgatagtc acgctgacat
     1021 ttcgggtgaa caacaccgcg ccatcggcga gcagttcggc gctggtgtcg gccagcgcgc
     1081 cgagcacgca ctcgcgcccg aaagcctcag cgagcagttc gttcttgccg gggccgttct
     1141 ggatggagaa ggcggcgtcg aaggcggcgt gacgcagcgc ggccagcgcc tgcgcggtgc
     1201 ctggcgtctt ggtcgcgacg atcagcacgc gcgccgcgct gagcgccgat ggatcgcgca
     1261 gcacaggcac cggcatcgtg aatgcggcga ggccgcggag ggtgaccccc tcgcgttcga
     1321 tctgcgcggc gcgttcgccg cgcgcaagca gcatgacgcg gtggccggcg cgcgcgagat
     1381 gcgcgccgag caccgagccc agggcgccgg cgccgaggat ggcgtagtcg ggcttatcca
     1441 cgatcagcgc cgcttcagca cgcgctgcct gtcgcgctgc cagtcgcggt ccttctcggt
     1501 cgcgcgcttg tcatgctgct tcttgccctt ggcgaggccg atcgagagct tggcgcggcc
     1561 cttctcccag tgcaggtcga gcggcacgat ggtgtagcct ttgcgctcga cggcgccgac
     1621 cagctggtcg agctcgcggc gattgagcag caacttgcgc gtgcgggtcg ggtcggcgtg
     1681 cacgtgggtc gaggtggtgt tgagcggcgt cacatgcgcg ccgatcagga aggcttcgcc
     1741 gccgcgcagg tagacatagg cttcggtgag attcgcgcgt ccggcgcgca gcgccttgac
     1801 ctcccagccc tcgagcgcga gacccgcgac gtagcggtcc tcgatgaagt agtcgaagcg
     1861 cgccttgcgg ttctcggcga tcggtcggtc ggaatttttt tccttggcca cgccgtattg
     1921 tagacaattg cgccatgcgg gaacggcagg tgacacgctc ggcgctggtg gcgatgccgc
     1981 cggcggcgat ctatgcgctg atcaacgaca tcgagtcgta tccgcagttc gtgccctggt
     2041 gcaggacggc ggtggtcgag tcggagagcg ataccgagat cgtcgcgacc ctcggtgtgc
     2101 gccggggtcc gctgcagacg gaattcacga cccgcaacat cctcgatccg gtgcgcagca
     2161 tcacgatgga gcacctgcgc ggcccgttca agcgcctgca cggcgcctgg accctggtac
     2221 cgatcggtga cgcgggcaca cgcatcgagc tcacgctgcg cttcgagttc gcgaaccggc
     2281 tgagcgcgtc gctgttcgat tccctgttcg aggagacggc gggtctgctg gtcgatgcgt
     2341 tcgtggcgcg tgcccgcagc ctgcgggacg cgccgccatg aagcgctgta cggtggctta
     2401 tgcgctcccg catgcccaat ggctgtggcc gctcgagttg cctcgggaag ccaccgtggc
     2461 ggatgcgctg cgcgccgtgc gcagcgcggt ggccagctcc ggcgcggcag gccagctgga
     2521 aatcgactgg gacggcgcaa ccgtcggcat ccatggtgcc gtctgcgcgc gcgaccagcg
     2581 tttcgccgat ggcgatcgca tcgaaatcta tcggccgctc ggcctcgacc cgcgcgcggc
     2641 gcgcagggcg cgcgtggcga atgcgcgccg ggcagggcgc cgttagccgg cgggattcgc
     2701 gtcgtcggtt tgcggcggcg cctggtcggg ctcgggggcc tgcacgggcg cgcgctccgt
     2761 atcctcggcc ggcggtgccg agggcgcagg agtggtcggc acgagcaccg gtgcgagcac
     2821 gttcaccttc tcgatgcgcg caactttgtc gtcctcgaac cagacggtga gccggcgccg
     2881 ttcgggctcc ttgatgcggc cttgcttgag aaagtagagg tagtcccagc gattctcgtc
     2941 gaacgcatcg ggcaccatcg gcgtgccgag caggaagcgt acctggctgc gggtcatgcc
     3001 ctcctgcaat ttggcgaccg catcggggtc gaggtaattg ccctgctgga tcgtcatgcg
     3061 gtagacgcag gcgccggcga gtccggacag ggcgaaggcg agcacggcga ggcgtgcgag
     3121 gcgggtcaag ggtggttgca tggtgcggat atgggggata atagactaat gataaccggg
     3181 agcacctgat ttggaaggca aagatctccg caaggccggt ctgaaggtga ccctgccgcg
     3241 actcaagatc ctcgagatcc tggagtcagg cacgccgcgc caccagagcg ccgagggcat
     3301 ctacaagcaa ctgctggagt cgaacgagga catcgggctc gccaccgtgt accgcgtgct
     3361 cacgcagttc gaggccgcgg ggctcgtcgt gcgccaccac ttcgagaacg gcatggcggt
     3421 gttcgagctg aacgagggcg cacaccacga ccacatcgtg tgcctcgact gcggcaaggt
     3481 cgaggagttc gtggaaaccg gtatcgagga gcgccagaac gcggtcgcgt cccggctcgg
     3541 gttcgagatc cgcgatcact cacttgttct ttacgggcgc tgtcgccgcc cgaactgccc
     3601 ctcgcgaaag ccgtagcatc tcgcgcgcgt gggcgcgcgc cttgtcggtc acttccacgc
     3661 cgcccagcat gcgtgcgagt tcctcgacgc gcgcgtcgcc gtcgagcgcg acgatctggg
     3721 tgcgggtcgt cctcccgtct gtgagcttgc tgacccgcag gtggtgatgg ccctgcgagg
     3781 cgacctgcgg caggtgggtg acgctcagca cctggctgcg atcgcccagc gcgcgcagtt
     3841 ccgcgccgac gatctcggcg accgccccac cgatgccggc atccacttcg tcgaacacca
     3901 tgcagcgacc ctcgcggcca ccgcgcgcga cctgcacggc gagcgacagg cgtgcgagtt
     3961 cgccgcctga ggcgaccttg gcgagcgcgc gcatcgggtt gccgggattg gcggtgaccc
     4021 ggaactcgac ctggtccagg ccgtgcggcg caggctccgg ggattcgagc ggcgacacgt
     4081 cgatctggaa ggcgccgccc gccatgccga gcgtctgcat gcgccccgtg atctccttcg
     4141 agagcgattt cgccgcggcc ttgcgtcgcg acgagagttg cccggcgcgc gtgcgccagg
     4201 cggcgagggc atccgcctgc tccttgcgca gggtggcgag ctgcgtgtcg gcgtgctcga
     4261 ggccggacag ctcggagcgc agttgcgtca ggcggttcgg cagttcggcg acgcccacgc
     4321 ggtgtttgcg cgccagttcc tcgaacgctg ccaggcgttt ctcgacttcg cccaggcgcg
     4381 cggtgtcgag atccaggccc tcggcatact gcgacagctc gcgcgccgct tcgcgcaggt
     4441 tgatcagcgc atcctcgacc atcggcgcga tgccggcgag ccgcggatcg tgcgcgccgg
     4501 cggccttcag cgccgcgagt gcgcggctgg tggaggcatg cgcgttcgcc tcctcgccct
     4561 cgtacagcag cgtgatcgcc ccctgcgccg cctcgatcag gcggccgcgg ttcgcgagcc
     4621 gcgagcgctc ctcggtcagc tgcgcgagct cgccttcctt catatcgagc gcctcgagct
     4681 cgcgcgcctg gaac
//
LOCUS       DAOQVY010000153         4647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_20600_length_4647_cov_4.985409, whole genome shotgun sequence.
ACCESSION   DAOQVY010000153 DAOQVY010000000
VERSION     DAOQVY010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20600_length_4647_cov_4.985409"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2128)
                     /locus_tag="PKN91_11460"
     CDS             complement(<1..2128)
                     /locus_tag="PKN91_11460"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNS28725.1"
                     /translation="MQAREHLNPTPPAAPDLTTRSMQSRKKTLLPTLILAVLVLNLAV
                     DGLLAFTLAEAKERKEGEVHDMVENMSLLLDLSVTASVEQIDLSLQEIATQLELAMRH
                     GAGRTPESTDHLLSLHQSRLARLGDLHVTDAEGAILLDRSATPRAPHGHALDDLIAAH
                     RSARDDRLIASRLLADEARGGWVVVFSRGYEDADGALAGLVTATLPAAYFQDRLSGLD
                     LGPGGIALIRDLEMHMVARIPPLDTAEGRIGSRGGSKELVDIVASGVQAQSFYSARTA
                     DGIHRINAFRRLSVMPAFVVVGLGEADYLAQWRDDVRKATFLALAFLVVSTLAGWLLW
                     RFISANERASQRSRILLQNASDGIHITDLEGRVIEASDAFCRMLGHPYADLLGMNVSS
                     WDVGQATTTWPQPKGPAPAEAQIRTSETEYRRADGSTMPVEITSYPLELDGRPVVFHS
                     ARDISARRKSEEEVRKLAFFDPLTELPNRRLLIQRLGETLSESTGTRVKGALLFIDLD
                     NFKTVNDTAGHYEGDRLLVRVARTLVECVSRNDVVARIGGDEFVVLLARLGTDEGTVT
                     RLADEVARRILNTLATSLGSGDGEYRASASIGVTLFDGEGEESAEAPLVRADVAMFAA
                     KLAGRNTIRFFDPRMQAALSERAELETGLWTALEQRQFFLSYQAQVLASGEVIGLEAL
                     LRWRHPQRGLVSPASFIPLAEETGLIV"
     gene            complement(2107..4032)
                     /locus_tag="PKN91_11465"
     CDS             complement(2107..4032)
                     /locus_tag="PKN91_11465"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNS28726.1"
                     /translation="MQAPRWPLRLAGAIVLLVLGAVMLWPYRSEDSPSPILLTPTERA
                     WLARHEPIRFISQSSYPPFEFVDAGGERRGMMIELVRWIATEAGFHAEFADAEFQVAQ
                     NAVREGKADVLTSLFFSEPRDRDFDFTATVYDVPASMFVRKTRTDLSTLPDLAGKRIA
                     IQRGDFAEQFLRTAGIAFELLPTANFAEATQAVIDGQADVLIGDEQIVFHHLFSAQHD
                     DRLKLVGDPLYVGRNAMAVREGEHVLLGVIDKGIEHARATGTLARLERKWLGLELRPR
                     KPDWRIYGPYALGALLVLFAVVVWNLRLEAVVRRKTADLEAKERRLQEIIEGTRAATW
                     VFDVATGAIEINEHWAEMLGYRVEELRPIRFETFRGFVHPDDWAHTQDRIDRHLAGEI
                     DHYASELRMRHRDGRWIWMLDRGRVTLRTEDGRPVVMAGIHIDISASKAHEDELQRMA
                     YFDGLTGLANRALLEDRLMQAIARARRSGELVVLGYIDLDGFKQINDRHGHGYGDRVL
                     MVIAQRLAQTLREEDTVARVGGDEFVFILREEDKIEAALQVVERLLGRIAEPIVLDAL
                     ELQVSASIGISACSGAEDIEAAQLVRQADDAMYQAKLSGKNRYHLFDRARNRPQPGEA
                     ASATQAGRRAGCRPAST"
     gene            4221..>4647
                     /locus_tag="PKN91_11470"
     CDS             4221..>4647
                     /locus_tag="PKN91_11470"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNS28727.1"
                     /translation="MEYTMPPAFTPVALEHASRLINHGPTVLVTAAHGGRRNLMAAAW
                     SMPVEFTPPRIAIVIDKNTFTRELVTASGRFGVCIPGAALADLTFAVGNCSGREGDKF
                     ARFGIAALPGPVLGVPLIEAGCAAWMECRLIPERHTEDAY"
BASE COUNT          684 a         1574 c         1634 g          755 t
ORIGIN      
        1 ggacgatcag gccggtctcc tcggcgagcg ggatgaagct cgcgggcgag accaggccgc
       61 gttgcggatg gcgccagcgc agcagcgctt cgagcccgat cacctcgccg gacgcgagca
      121 cctgcgcttg atagctcagg aagaactgcc gctgctcgag cgcggtccac agcccggtct
      181 cgagttccgc gcgctcgctc agtgcggcct gcatccgggg atcgaagaaa cggatcgtgt
      241 tgcggccggc cagcttggcc gcgaacatcg cgacgtccgc acgcacgagc ggcgcttcgg
      301 cgctctcctc gccctcgccg tcgaacaagg tcacgccgat gctggccgag gcccggtatt
      361 cgccgtcgcc cgagcccagg ctggtcgcga gcgtgttgag gatcctgcgc gcgacttcgt
      421 ccgccagccg cgtcaccgtg ccctcgtccg tacccagcct ggccagcagc accacgaact
      481 cgtccccgcc gatgcgggcg acgacgtcgt tccgggacac gcactcgacg agggtgcgcg
      541 cgacccgtac gagcaggcgg tcgccctcgt agtggccggc ggtgtcattg accgtcttga
      601 agttgtcgag gtcgatgaac agcagtgcgc ccttgacccg ggtgccagtg ctctcgctca
      661 aggtctcccc gagacgctgg atgagcaggc ggcggttggg cagttcggtg agcgggtcga
      721 aaaaggcgag cttgcggacc tcttcctcgg atttcctgcg cgccgagatg tcgcgcgccg
      781 aatggaagac gaccgggcgg ccatcgagct cgagcggata ggaggtgatc tcgaccggca
      841 tcgtgctgcc atcggcacgg cggtattccg tttccgaggt gcggatctgc gcctcggcgg
      901 gcgcaggacc tttcggctgc ggccatgtgg tcgtcgcctg gccgacatcc cagctcgaga
      961 cgttcatgcc gagcaggtcg gcgtacggat ggcccagcat ccggcagaag gcatcgctcg
     1021 cctcgatcac ccggccttcg agatcggtga tgtggatgcc gtcgctcgcg ttctgcagca
     1081 ggatccggct gcgctggctt gcgcgctcgt tcgcgctgat gaaacgccac agcagccagc
     1141 ctgccagcgt gctcaccacg aggaaggcga gggcgaggaa ggtggccttg cgcacgtcgt
     1201 cccgccactg cgcgaggtag tccgcctcgc ccaggccgac cacgacgaag gcgggcatca
     1261 ccgacaagcg tcggaacgcg ttgatgcgat ggatgccgtc ggccgtgcgc gcgctgtaga
     1321 aggactgcgc ctgcacgccc gatgccacga tgtcgaccag ctccttcgag ccgccccggc
     1381 tgccgatgcg gccctcggcg gtgtcgagcg gggggatgcg cgcaaccatg tgcatttcca
     1441 ggtcgcggat cagcgcgatc ccgccggggc ccaggtcgag tccggagagc cgatcctgga
     1501 aatacgcggc cgggagcgtc gcggtgacca ggccggcgag ggcgccgtcg gcgtcctcgt
     1561 agccgcgtga gaagaccacg acccagcccc cgcgggcctc gtcggccagc aggcggctgg
     1621 cgatgagccg atcgtcccgc gcggaccggt gtgcggcgat caggtcgtcc agcgcatggc
     1681 cgtgtggtgc tctcggcgtt gcggaccggt ccagcaggat cgccccctcg gcgtcggtga
     1741 cgtggaggtc gcccagtcgg gcgagtcggc tctgatgaag cgagagcagg tggtccgtgc
     1801 tctccggtgt gcggcccgca ccgtgccgca tcgccagctc cagctgcgtc gcgatctcct
     1861 gcagggacag gtcgatctgc tccaccgagg ccgtcacgct caggtcgagc agcagggaca
     1921 tgttctcgac catgtcgtgg acctccccct ccttgcgctc cttggcctcg gcaagggtga
     1981 aggcgaggag cccgtcgacc gccaggttga gcacgagtac cgcgaggatc agcgtgggga
     2041 ggagggtctt cttgcgactt tgcatcgagc gggtggtgag atccggggct gcgggcggtg
     2101 ttgggttcag gtgctcgcgg gcctgcatcc ggcacgcctg ccggcttgcg tcgcggaggc
     2161 ggcttcgccg ggctgcgggc ggttgcgggc acgatcgaag aggtggtagc ggttcttgcc
     2221 gctcagcttg gcctggtaca tggcatcgtc ggcctgacgg acgagctgcg ccgcttcgat
     2281 gtcttccgcg ccggaacagg cgctgatgcc gatgctggcg gaaacctgca gctcgagcgc
     2341 gtcgagcacg atcggttcgg cgatgcgccc gagcaggcgc tcgacgacct ggagcgcggc
     2401 ctcgatcttg tcctcctcgc gcaggatgaa gacgaactcg tcgccgccca cgcgcgcgac
     2461 cgtgtcttcc tcgcgcaggg tctgcgccag gcgttgcgcg atgaccatca gcacgcgatc
     2521 gccgtagccg tgcccgtgcc ggtcgttgat ctgcttgaag ccgtcgaggt cgatgtagcc
     2581 gagcaccacc agctcgccac tgcggcgcgc gcgtgcgatc gcctgcatga ggcggtcttc
     2641 gagcagggcg cggttcgcca gtccggtcag gccgtcgaaa taggccatgc gctgcagttc
     2701 gtcctcgtgt gccttgctcg ccgagatgtc gatatggatc cccgccatca cgacgggtct
     2761 tccgtcctcc gtccgcagcg tcacgcggcc gcgatccagc atccagatcc agcgcccgtc
     2821 gcggtgacgc atgcgcagct cgctggcgta gtggtcgatc tctccggcga ggtggcggtc
     2881 gatccggtcc tgcgtatgcg cccagtcgtc gggatggacg aagccgcgga aggtttcgaa
     2941 ccggatgggg cgcagctcct cgacacgata gccgagcatc tccgcccagt gctcgttgat
     3001 ctcgatcgcg ccggtggcga cgtcgaagac ccaggtcgcc gcgcgcgtgc cctcgatgat
     3061 ctcctgcagc cgccgctcct tcgcctccag gtcggccgtc ttgcgccgca cgactgcctc
     3121 gaggcgcagg ttccacacca ccaccgcgaa cagcaccagc agcgcgccga gcgcgtaggg
     3181 gccgtagatg cgccagtcgg gcttgcgggg ccgcagctcc aggccgagcc acttgcgctc
     3241 caggcgcgcc agcgtgccgg tggcgcgggc gtgttcgatg cccttgtcga tcacgcccag
     3301 gaggacgtgc tcgccctcgc gcaccgccat cgcattgcgg ccaacgtaca aggggtcgcc
     3361 gaccagcttg agccgatcgt cgtgctgcgc gctgaagaga tgatggaaga cgatctgctc
     3421 gtcgccgatc agcacgtcgg cctgaccgtc gatcaccgcc tgcgtcgcct cggcgaagtt
     3481 cgcggtgggc aggagctcga aggcgatgcc ggccgtgcgc aggaactgct cggcgaagtc
     3541 gccgcgctgg atggcgatgc gctttccggc gaggtcgggg agggtggaga ggtcggtgcg
     3601 cgtcttgcgc acgaacatcg acgccggcac gtcgtagacg gtggccgtga aatcgaaatc
     3661 gcggtcgcgc ggctcgctga agaacaggct ggtgagcacg tcggccttgc cctcgcgcac
     3721 ggcattctgc gcgacctgaa actccgcgtc ggcgaactcg gcatgaaacc cggcctcggt
     3781 cgcgatccag cgcacgagct cgatcatcat cccgcgccgc tcaccgcccg catcgacgaa
     3841 ctcgaacggc gggtagctgc tctggctgat gaagcggatc ggctcgtggc gggcgagcca
     3901 ggcgcgttcg gtgggggtca gcaggatcgg actcggcgag tcctcgctcc gatagggcca
     3961 gagcatgacc gcgccgagca cgagcagcac gatcgccccg gcgaggcgga ggggccagcg
     4021 aggagcctgc acgaagagtc ctgatcggtt cgagttattt tgcgcggatt atcccggtgt
     4081 ctcgccccgc cggtcaaaat gcttcgcagc tcgggtgtga gccagttcgc ggacacgctt
     4141 gcgctgccgc cgctatccgg cgcaggcccg gcgccgcggt cctgctgaat catggaaaat
     4201 ggcggcctcc aggatgcccc gtggaataca ccatgccccc cgccttcacc cccgtcgccc
     4261 tcgaacatgc cagccgcctg atcaaccacg gccccacggt gctcgtcacc gctgcccacg
     4321 gcgggcggcg caacctgatg gcggcggcgt ggtcgatgcc ggtggagttc accccgccgc
     4381 gcatcgcgat cgtgatcgac aagaacacct tcacccgcga gctggtcacg gccagcggcc
     4441 ggttcggcgt ttgcattccc ggcgcggcgc tggccgacct gactttcgcg gtggggaact
     4501 gcagcgggcg cgagggcgac aagttcgcgc gcttcggcat cgccgcgctg cccgggccgg
     4561 tgttgggcgt gcccctgatc gaggcgggct gtgcggcgtg gatggagtgc cgcctgatcc
     4621 ccgagcgcca caccgaggac gcctacg
//
LOCUS       DAOQVY010000154         4636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_20671_length_4636_cov_6.024012, whole genome shotgun sequence.
ACCESSION   DAOQVY010000154 DAOQVY010000000
VERSION     DAOQVY010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20671_length_4636_cov_6.024012"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /locus_tag="PKN91_11475"
     CDS             complement(<1..1393)
                     /locus_tag="PKN91_11475"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNS28728.1"
                     /translation="MALLSGALAACSPQSSTAASAAQSASGAPASVAAAPPAERTAPL
                     VQGLPDFAPLVEAYGPAVVNVSVVSRRESRGGFQGPPGMSPDDPFYEFFRRFGIPTPR
                     GELPPARGEGSGFIVSPDGYILTNAHVVDGASEVTVRLTDRREFTAKVIGSDKRTDIA
                     VLKIEADGLPTVKIGDPSRLRAGEWVIAIGSPFGFENSVTAGIVSGVSRSLPSDNYVP
                     FIQTDAAVNPGNSGGPLFNLAGEVVGINSQIYSRTGGYMGVSFAIPIDVATSVRDQLI
                     STGRVSRGRIGVTVQEVNAQFAESFGLDRPRGALVGSVEAGGPGDKGGLKPGDVILAV
                     DGTPIERSAQLPGVIASIKPGSEAKLEVWRDKRSRKLTVKVAELDEPVQRVSMGDADP
                     TDNYSKLGLAVRALDAREKAMTGGSGSLVIEGVEGPAAFAGLQRGDIILGVNGQSVDS
                     VSELQAAVRKAGKA"
     gene            complement(1495..3024)
                     /gene="lnt"
                     /locus_tag="PKN91_11480"
     CDS             complement(1495..3024)
                     /gene="lnt"
                     /locus_tag="PKN91_11480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNS28729.1"
                     /translation="MTGTEAASRAGDSLAARRWAAPLAAAAGASVALAFAPFGLWPFA
                     IIGPAVLIAMWDGAAPRRAAVLGFWFNAGTFAAGTYWLYISIHVFGGAPLWIAVLLML
                     GLVAIMGLYHAALGFAVARWLPARGAWRWLAGLPAAWLLVEWLRGWLFSGFSWLSLGY
                     AQTDTWLARLAPVGGVYAVSAATLVSAGALVMLARARGVRPRGLAVAALVLPWLLALA
                     LDRDWTRPSGAPVRVAVLQGAIPQDMKWLEDNLDTTLELYAQLTREAIGDPLIVMPES
                     ALPDLVNNLLPFLGTLYEEASARGSSLVLGVVRAGDERDQYFNSVVSLSGEGVGWYDK
                     HHLVPFAEFFPVPQFVRSWLRLMSLPYSDFTRGPAVQPPLRAAGLSLAAGICYEDAYG
                     STLARSLAQADTLVNVTNDAWFGRSSARFQHFQIARMRAIESGRYLIRAANDGITAVI
                     GPRGAVVAQAPEYTATVLRASVTPHGGLPPYARVGNFAIVLLAAAALAAALLAGKGSQ
                     A"
     gene            complement(3011..3901)
                     /locus_tag="PKN91_11485"
     CDS             complement(3011..3901)
                     /locus_tag="PKN91_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNS28730.1"
                     /translation="MTRDPNDTTDGNATGRWLKRLKRSVGGEPEDRESLLNSLRAAAA
                     RGLLDADALEMIEGVLDVADLRVRDIMIPRAQMVSVRRNDPASAILPAVVESGHSRFP
                     VMDDDRDDIVGILLAKDLLRVFAEGGTDSFDIRECMRPAVFVPEAKRVNVLLKEFRGN
                     RNHMAIVVDEYGGVAGVVTIEDVIEQIIGEIDDEFDVDEDINIRKDAERQFTVRGVTE
                     IEEFNEYFGSDFPDDEFDTVAGLVMKAFGRLPRRGESVTIGDIEFRVMRADRRRLESL
                     RVSTTRDVAAPEERPASDGY"
     gene            complement(3903..4364)
                     /gene="ybeY"
                     /locus_tag="PKN91_11490"
     CDS             complement(3903..4364)
                     /gene="ybeY"
                     /locus_tag="PKN91_11490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNS28731.1"
                     /translation="MTPALEVEVAHGTRAWAPAPAAMTRWARAALGRRGTGRQLAVRV
                     VAPAESRRLNAAWRGRDKPTNVLSFPAAGLATGGARPLGDLVICAAVVRGEAREQGKP
                     LADHWAHLIVHGALHLVGYDHEGDDDARRMERREIAVLRRLGIQNPYLTRE"
     gene            complement(4361..>4636)
                     /locus_tag="PKN91_11495"
     CDS             complement(4361..>4636)
                     /locus_tag="PKN91_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNS28732.1"
                     /translation="LDEAQNTTIEQMKMFLTRIGFGSRAVVTGDVTQTDLPTGRQSGL
                     RHVIDVVQGVEGVAFTFFDAQDVVRHPLVQRIVQAYEARGDTGRSAP"
BASE COUNT          722 a         1672 c         1597 g          645 t
ORIGIN      
        1 cggccttgcc ggccttgcgc accgcggcct gcagttcgct caccgagtcg accgactggc
       61 cgttcacgcc aaggataatg tcgccgcgct gcaggccggc gaaggcggcc ggcccctcca
      121 cgccctcgat caccagcgaa ccgctgccgc cggtcatcgc cttctcgcgc gcgtcgaggg
      181 cgcgtaccgc gagcccgagc ttcgagtagt tgtcggttgg atcggcatcg cccatcgaga
      241 cccgctgcac cggctcgtcg agctcggcca ctttcaccgt gagcttgcgg ctgcgcttgt
      301 cgcgccacac ctcgagcttc gcctcgctgc cgggcttgat gctcgcgatg acgccgggca
      361 gctgcgccga gcgctcgatc ggcgtgccgt cgaccgcgag gatcacgtcg ccgggcttga
      421 gcccgccctt gtcgcccggg ccgcccgcct ccaccgaacc gaccagcgca ccgcgcggcc
      481 ggtcgaggcc gaaggactcg gcgaactgcg cgttcacttc ctgcaccgtg acaccaatgc
      541 gaccgcggct cacgcgcccg gtgctgatca actggtcgcg cacgctggtc gccacgtcga
      601 tcgggatcgc gaacgacacg cccatgtagc cgccggtgcg gctgtagatc tgcgaattga
      661 tgccgacgac ctcgccggcg agattgaaca gcgggccgcc cgagttgccc ggattcacgg
      721 ccgcgtcggt ctggatgaat ggcacgtagt tgtcggacgg cagcgaacgc gacacgccgc
      781 tgacgatgcc cgcggtcacg ctgttctcga atccgaacgg cgagccgatc gcaatcaccc
      841 attcgccggc gcgcagccgg ctcgggtcgc cgatcttgac ggtcggcagc ccgtccgcct
      901 caatcttgag cacggcgatg tcggtgcgct tgtcgctgcc gatcaccttg gccgtgaact
      961 cgcggcgatc ggtcaggcgg acggtcacct cgctcgcgcc atcgaccaca tgggcgttgg
     1021 tgaggatgta accgtccggc gagacgatga agcctgagcc ctcgccgcgg gcgggcggca
     1081 gctcaccgcg cggcgtcgga atgccgaagc ggcggaaaaa ctcgtagaac ggatcgtcgg
     1141 gcgacatgcc cggcggaccc tggaagccgc cgcgcgactc gcgccggctc accacgctca
     1201 cgttgacgac ggccgggccg tacgcctcga ccagcggcgc aaagtccggc agtccctgca
     1261 cgagcggcgc ggtgcgctcg gccggcgggg cggccgccac gctcgcgggt gcgcccgacg
     1321 cggactgcgc ggccgatgcc gcggtcgatg actggggcga acaggcggcg agcgcgccgc
     1381 tcaaaagggc cacggcggca atccgccggg caaggggctg tgatgccatt cgttgaatgt
     1441 ccgatatggt ggtgacgagg aaggtagacc ttcggagcac cgtttcagtt ccgttcatgc
     1501 ctgggacccc ttaccggcaa gcagcgcggc cgcgagcgcg gcggccgcga gcaggacgat
     1561 ggcgaaattg cccacccgcg cgtagggcgg caggccgccg tgcggcgtga cgctggcgcg
     1621 cagcactgtg gcagtgtact cgggcgcctg cgccaccacg gccccgcgcg gaccgatgac
     1681 ggcggtgatg ccatcgttcg cggcgcggat gagatagcgc ccggactcga tcgcgcgcat
     1741 gcgcgcgatc tggaagtgct ggaagcgcgc ggacgagcgc ccgaaccacg cgtcgttggt
     1801 cacgttgacg agcgtgtcgg cctgcgcgag cgatcgcgcg agcgtgctgc cgtaagcgtc
     1861 ctcgtagcag atgccggccg cgagcgacag ccccgcggcg cgcagcggcg gctgcacggc
     1921 cgggccgcgc gtgaaatccg aatacggcag gctcatcagg cgcagccagc tgcgcacgaa
     1981 ctgcggcacc ggaaagaatt ccgcgaacgg caccagatgg tgcttgtcgt accagccgac
     2041 gccttcgcca ctgagcgaca cgaccgagtt gaagtactgg tcgcgctcgt cgccggcgcg
     2101 taccacgccg agcaccagcg acgaaccccg cgcgctcgcc tcctcataga gggttccgag
     2161 gaagggcagc aggttgttga cgaggtcggg cagcgccgac tccggcatga cgatgagcgg
     2221 atcgccgatc gcctcgcgcg tgagctgcgc gtacagctcg agcgtcgtat cgaggttgtc
     2281 ctcgagccac ttcatgtcct gcgggatcgc gccctgcagc accgcgacgc gcaccggcgc
     2341 gccgctcggg cgcgtccagt cgcgatccag cgcgagcgcg agcagccatg gcagcacgag
     2401 cgccgccacg gcgaggccgc gcgggcgcac gccgcgggcg cgcgcgagca tcacgagcgc
     2461 acccgccgag acgagcgtgg ccgccgacac ggcgtagacg ccgccgaccg gcgcgagacg
     2521 cgcgagccag gtatcggtct gcgcatagcc gagcgacagc caagagaagc cggagaacag
     2581 ccagccgcgc agccactcga ccagcaacca cgcggccggc agaccggcga gccagcgcca
     2641 ggcgccgcgc gccggcagcc agcgtgcgac cgcaaacccc agcgcggcgt ggtacaggcc
     2701 catgatcgcg accaggccca gcatcagcag taccgcgatc cacagcggtg cgccgccgaa
     2761 gacgtggatg ctgatataga gccagtaagt gccggccgcg aaggtaccgg cgttgaacca
     2821 gaagccgagc acggcggcgc ggcgcggcgc ggccccgtcc cacatcgcga tgagcaccgc
     2881 cgggccgatt atcgcgaacg gccagaggcc gaagggcgcg aaggccagtg cgaccgaggc
     2941 cccggcggcc gcggccagcg gtgccgccca gcggcgcgcg gcgaggctgt cgcctgccct
     3001 gctcgccgcc tcagtacccg tcacttgccg gccgctcctc gggcgccgcg acatcccgtg
     3061 tcgtgctgac acgcaggctc tcgaggcgcc tgcggtccgc gcgcatcacg cgaaactcga
     3121 tgtcgccgat cgtgaccgac tcgccgcgac gcggcaggcg accgaaggcc ttcatcacca
     3181 gtccggcgac ggtgtcgaac tcgtcatccg ggaaatccga tccgaaatat tcgttgaatt
     3241 cctcgatctc ggtgacgccg cggacggtga actgtcgctc ggcgtccttg cggatgttga
     3301 tgtcctcgtc gacgtcgaac tcatcgtcga tctcaccgat gatctgctcg atcacgtcct
     3361 cgatcgtgac gacgccggcc acgccgccgt actcgtcgac cacgatcgcc atgtggttgc
     3421 ggttgccgcg gaattccttc agcagcacgt tgacccgctt cgcctcgggc acgaacactg
     3481 ccgggcgcat gcactcgcgg atgtcgaaac tgtcggtgcc gccctcggca aagacgcgca
     3541 gcaggtcctt cgcgagcagg atcccgacga tgtcatcacg gtcgtcgtcc atcaccggaa
     3601 agcgtgagtg gccggattcg accaccgccg gcaggatcgc ggacgccggg tcgttccgcc
     3661 gcacggacac catctgggcg cgcggaatca tgatgtcgcg cacgcgcagg tcggccacgt
     3721 cgagcacacc ctcgatcatc tcgagtgcat cggcatcgag caggccacgc gccgcggccg
     3781 cccgcaggct gttgagcagc gactcgcgat cctcgggctc gcccccgacg gagcgcttca
     3841 gccgcttgag ccagcgcccg gtcgcattgc cgtcggtggt gtcgttcggg tccctcgtca
     3901 tgtcactccc tggtgagata cggattctga ataccgagcc ggcgaagcac ggcgatctcg
     3961 cgccgctcca tgcgccgcgc gtcgtcgtcg ccctcgtggt cgtagccgac gaggtgcagt
     4021 gcaccgtgca cgatcaggtg cgcccagtga tcggcaaggg gcttgccctg ctcgcgcgcc
     4081 tcgccccgga ccaccgcggc gcagatcacc aggtcgccga gcggccgcgc acccccggtg
     4141 gcgagccccg cggccggaaa tgacagcacg ttcgtgggct tgtcccggcc gcgccaggcg
     4201 gcgttcagcc gccggctctc ggcgggcgcc accacgcgca ccgcgagctg gcgccctgtg
     4261 ccgcgccgcc cgagggccgc acgggcccag cgggtcatcg cggccggcgc cggcgcccag
     4321 gcgcgcgtgc cgtgcgcgac ttcgacctcg agtgcaggcg tcatggggcg ctgcgcccgg
     4381 tgtcgccgcg cgcctcgtag gcctgcacga tccgctgcac gagcgggtgg cgtacgacgt
     4441 cctgcgcatc gaagaacgta aacgccacgc cctcgacgcc ctgcacgaca tcgatgacgt
     4501 ggcgcaggcc cgactgacgg ccggtcggca ggtcggtctg cgtgacgtcg ccggtgacca
     4561 cggcccgcga gccgaagccg atgcgggtca ggaacatctt catctgctcg atcgtcgtgt
     4621 tctgcgcctc gtcgag
//
LOCUS       DAOQVY010000155         4561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_21115_length_4561_cov_5.062361, whole genome shotgun sequence.
ACCESSION   DAOQVY010000155 DAOQVY010000000
VERSION     DAOQVY010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21115_length_4561_cov_5.062361"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1674
                     /locus_tag="PKN91_11500"
     CDS             148..1674
                     /locus_tag="PKN91_11500"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNS28733.1"
                     /translation="MASLQSIEQWDDETDVLIAGYGVAGCAAAIEAHDSDPAADILIV
                     EKMPAERAGGNGRASGQSLLISHNAEALYRYQKAMSATNPPPDDMLREWARQMAGLEE
                     YIEARVAEAGNTQLVKGSGWSSGEAVYEFPEFGAEDAVAYNAFVKPIPSGVWITMRTC
                     VEKRPRVRHVFESPLVDLVQDPDTLEVFGAIVETRGMRRAIRARRGVIVAVGGYEADM
                     EMQRNYYGLAEAWPLGTPANTGDGVRVLQKAGAELWHMRNFGQSGGIWPGIKVPEFPT
                     IFMRRHFWQTFSWIEIGAEDRRFYNEGAELYHTHYKERHHGHWVDTPHWRAGPVHMLF
                     DENVREHNCLVTHAMSWNTVVEGYEWSDDNSVEVDKGWIVKADSIEALAAKIGRDPAR
                     VAAEVARFNAACERGEDTDFGRPAHSLQPLVTPPYYAVRIDLAIVCTSGGAKRNILSQ
                     VLDPRGQPIARLHEAGELGSMISHLYQNGSYLTEAMISGRAAGRSAVMLDPWKMKRVA
                     "
     gene            1675..1899
                     /locus_tag="PKN91_11505"
     CDS             1675..1899
                     /locus_tag="PKN91_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28734.1"
                     /translation="MRVVTPAMKLDVRIDELAAKDGLLQMSGVAGMLPCETTLRPSEL
                     RRMIGKLMRPSILRLLLSGDPPVPGKPPAR"
     gene            complement(1975..2772)
                     /locus_tag="PKN91_11510"
     CDS             complement(1975..2772)
                     /locus_tag="PKN91_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNS28735.1"
                     /translation="MKRIAILGSGTPCAAGAPAHLSAMTPPGIELVPWPLPISAFPHT
                     PFDHVLVAASHAAAAAEALAGGADALYIDTFGDYAINAMRALGDAPVIGAGEATLHAA
                     TLVARRFSIVTVWPRSMRFIYDERLAASGLAARCASIRQLGEPVDVMARLQGGDGNVT
                     ARIVEACREAIEHDGAEAIALGCTCMAPVHAAVAQAIDAPVLCCSRTGYAMAIAAVLG
                     GWPSSALAWPRVPRQRVERLLALASGSGAAQAGGDDACPVCPTTPEG"
     gene            complement(2769..2921)
                     /locus_tag="PKN91_11515"
     CDS             complement(2769..2921)
                     /locus_tag="PKN91_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28736.1"
                     /translation="MRFLGVALMPLELGPQPFCVANGGLRDRFDARRAEVEARFRMVL
                     DGARSL"
     gene            3027..4556
                     /locus_tag="PKN91_11520"
     CDS             3027..4556
                     /locus_tag="PKN91_11520"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNS28737.1"
                     /translation="MIGPGLLLSGGLRVSAARTPSKVALICAQQRRTYAELLGRVNRV
                     GARLAALGFRPGDRAIVLAPNCIEYPELICGVADAGGVVATLSARSATAEVTAAANDC
                     GARVLFVHEANLGTVDRSALPLVEHVIVLGEEYEAWLRAADPAFTPMLSAAGAPVAET
                     DPFTLVYTSGTTGRPKGILISHRSRTLTFHAMAMEYGCYGPDDLQLGIAPMAHGAGFA
                     FIMCSVYFGGTVEILPKFDAELVVGKLAEGEFTGVFMVPTHYSSIFGLGPGVLERHRG
                     KARALRTIMSNAAALPQVLKEKIVGYWGPGLLHETYGSTEAGIVTNLRPQYQLEKPRC
                     VGLPFALNEVRLLDQAGRDVAEGEVGELYSRSPYLFNGYYNQPEETAAVWRDGWLTAG
                     DLARRDADGHYYIVDRRKDMVVSGGLNVYPREIEEVLQAHPAVHEAAVIGVPDEHWGE
                     RLCAYFVLRRERHAGSDELEAWCRERLAGYKVPKEFRALAELPRNAGGKVLKKELRQL
                     S"
BASE COUNT          753 a         1490 c         1626 g          692 t
ORIGIN      
        1 gagaggccga ggtgttggcg gagagcaagg ggccgaggac ccgcgcgagc tgacgcaggc
       61 gattgcgtgc ccgccggttg ccgcgggccg gcaagttcac ggacatgaat ttgacagcgc
      121 cgctgcgttt ctggcgggat aggcttcatg gccagcctgc aatcgatcga gcagtgggat
      181 gacgagaccg acgtcctgat cgccggatac ggcgtcgccg gatgcgctgc ggcgatcgag
      241 gcgcacgaca gcgatccggc cgccgacatc ctgatcgtcg agaagatgcc cgcggagcgc
      301 gcgggcggca atggccgcgc gtcggggcag tcgctgctca tctcgcacaa cgcggaggcg
      361 ctttaccgct accagaaggc gatgagcgcg accaacccgc cgcccgatga catgctgcgc
      421 gagtgggcgc gacagatggc gggactcgag gaatacatcg aggcgcgcgt cgccgaggcg
      481 ggcaacaccc agctcgtgaa aggcagcggc tggagctcgg gcgaggcggt gtacgagttt
      541 cccgagttcg gcgccgagga cgcggtggcg tacaacgcgt tcgtgaagcc gatcccgagc
      601 ggcgtgtgga tcacgatgcg cacctgtgtc gagaagcgcc cccgcgtccg ccacgtgttc
      661 gagtcgccgc tcgtcgacct ggtgcaggac cccgatacgc tcgaagtgtt cggcgcgatc
      721 gtcgagacgc gcggcatgcg gcgcgcgatt cgcgcacggc gcggcgtgat cgtggcggtc
      781 ggtggctacg aggccgacat ggagatgcag cgcaactact acgggctcgc ggaagcctgg
      841 ccgctcggca cgccggccaa cacgggcgat ggcgtgcgcg tcctgcagaa ggccggtgcg
      901 gagctgtggc acatgcgcaa tttcggccag tcgggcggca tctggcccgg catcaaagtg
      961 cccgagttcc cgaccatctt catgcgccgc catttctggc agaccttcag ctggatcgag
     1021 atcggcgccg aagatcggcg cttctacaac gagggcgccg agctctatca cacgcactac
     1081 aaggagcgac accacggcca ctgggtcgac accccgcact ggcgcgcggg ccccgtgcac
     1141 atgctgttcg acgaaaacgt gcgcgagcac aactgcctcg tcacgcacgc gatgagctgg
     1201 aacaccgtgg tcgagggcta cgaatggagc gatgacaaca gcgttgaggt ggacaagggc
     1261 tggatcgtga aggccgactc gatcgaggcg ctcgccgcga agatcggccg cgatccggcg
     1321 cgcgtggcgg ccgaggtcgc gcgcttcaac gcggcctgcg agcgcggcga ggacacggac
     1381 ttcgggcggc ccgcccactc actgcagccg ctcgtcacgc cgccgtacta cgccgtcagg
     1441 atcgatctgg cgatcgtgtg cacgagcggc ggggcaaagc gcaacatcct ctcgcaggta
     1501 ctcgacccgc gcggccagcc gattgcgcgg ctgcacgagg ccggcgagct cggctcgatg
     1561 atctcgcacc tgtaccagaa cggctcctac ctcaccgaag cgatgatttc cggtcgcgcg
     1621 gcggggcgca gcgcagttat gctggacccc tggaagatga agagggtcgc ctagatgcgc
     1681 gtggtcaccc cggcgatgaa actggacgtg cggatcgatg aactcgccgc aaaggacggc
     1741 ctgctgcaga tgagcggggt ggccggcatg ctgccttgcg agactacgct tcggccctcc
     1801 gaactgcgcc ggatgatcgg caagctgatg cgcccgtcga tcctcagact gctgctgagc
     1861 ggcgatcctc cggtgcccgg caagccaccg gcgcgttgac gcgcacccgc gcacgatacc
     1921 tgcgtatcgc ggcgaggatg tcgcgcgcgt actcccgctc gaactcgcgt acgctcagcc
     1981 ctccggggtg gtggggcaca ccgggcaggc gtcgtccccg ccggcctgcg ccgcgccgct
     2041 gccgctcgca agtgccagca gtcgttcgac gcgctggcgc ggtacgcgcg gccaggcgag
     2101 cgcgctcgat ggccagccgc cgaggaccgc cgcgatcgcc atggcatacc cggtgcgcga
     2161 gcaacacagc accggggcat cgatggcttg ggctaccgcg gcgtgcaccg gtgccatgca
     2221 cgtgcagccg agcgcaatgg cttcggcgcc gtcgtgttcg atggcctcgc ggcacgcctc
     2281 gacgatgcgc gccgtcacgt tcccgtcccc gccctgcagg cgcgccatga cgtcgaccgg
     2341 ctcgccgagt tggcggatcg acgcgcagcg cgccgcaagt ccgctcgccg ccagtcgctc
     2401 gtcgtagatg aagcgcatcg agcgtggcca caccgtcacg atcgagaagc gccgcgccac
     2461 gagcgtcgcc gcgtgcagcg ttgcctcgcc ggcgccgatc acgggcgcat cgccgagggc
     2521 gcgcatggcg ttgatcgcgt agtcgccgaa ggtatcgatg tacagtgcat cggcgccgcc
     2581 ggcgagcgcc tcggcggccg cggctgcgtg gcttgcggcc acgagcacat gatcgaaggg
     2641 tgtgtgcggg aaggcggata tcggcagcgg ccagggcacg agctcgatgc cgggcggagt
     2701 catcgccgaa agatgcgccg gggcgccggc tgcgcagggc gtgccactgc cgaggatcgc
     2761 gatccgtttc atagcgagcg cgcgccgtcg aggaccatgc ggaaacgcgc ctcgacttcg
     2821 gcacggcgtg catcgaagcg atcgcgcagt ccgccgttgg cgacgcaaaa gggttgcggg
     2881 ccgagctcga gcggcatcag tgcgactccc aaaaaccgca tatctggaac atcggtacga
     2941 aattccgaac ttgattgaca ggagtctgag cccgatgcca ccgtaagtcg agacaccata
     3001 ggaaacgacg gacgggattt caggcgatga tcggcccagg actgctactg tcgggggggt
     3061 tgcgggtctc ggccgcgcgc acgccgtcga aggtcgcgct catctgcgcg cagcaacgtc
     3121 ggacctatgc cgagttgctc gggcgcgtga accgcgtcgg cgcgcgcctt gccgcactgg
     3181 gcttccggcc gggcgatcgc gccatcgtgc tcgcgccgaa ctgcatcgag tacccggagc
     3241 tgatctgcgg cgtcgccgac gccggcggcg tcgtcgcgac actgagtgcg cgttccgcga
     3301 ccgcggaggt caccgcggcc gccaatgact gtggagcgcg cgtgctgttc gtgcacgagg
     3361 cgaacctcgg cacggtcgat cgatcggcgc tgccgctggt cgagcacgtg atcgtgctcg
     3421 gcgaggagta cgaggcctgg ctgcgcgcgg cggatcctgc attcacgcca atgctgtcgg
     3481 ctgcgggcgc gccggttgcc gagaccgatc cgttcacgct tgtctacaca tccggcacga
     3541 ccggccggcc caagggtatc ctcatctcgc accgttcgcg cacgctgaca ttccacgcga
     3601 tggcgatgga atacggctgc tacggcccgg acgacctgca gctcggcatc gcgccgatgg
     3661 cgcacggcgc gggcttcgca ttcatcatgt gttcggtgta tttcggcggg acggtcgaga
     3721 tcctgccgaa gttcgatgcc gagctcgtcg tcggcaagct ggcggagggc gagttcaccg
     3781 gcgtcttcat ggtgcccacc cactactcct cgatcttcgg gctcgggcct ggggtgctcg
     3841 agcgccatcg cggcaaggca cgcgcgctgc gcacgatcat gtcgaatgca gccgcgttgc
     3901 cgcaggtgct caaggagaag atcgtcggct actgggggcc gggtctgctg cacgagacct
     3961 atggctcgac cgaggccggc atcgtcacga atctgcgccc gcagtaccag ctcgagaagc
     4021 cgcgctgcgt cggcctgccg ttcgcgctca acgaagtgcg cttgctcgat caggctggcc
     4081 gggatgtcgc ggagggtgag gtgggtgagc tgtattcacg ctcgccgtac ctgttcaacg
     4141 gctactacaa ccagccggag gagaccgccg cggtgtggcg ggacggctgg ctgacggccg
     4201 gcgatctcgc gcggcgcgac gccgacggtc attactacat cgtcgatcgt cgcaaggaca
     4261 tggtggtctc gggcgggctc aacgtgtacc cgcgcgagat cgaggaggtg ctgcaggcgc
     4321 atcccgccgt gcacgaagca gcggtgatcg gcgtgcccga tgaacactgg ggcgagcggc
     4381 tgtgtgcgta cttcgtcctg cggcgggagc gacatgccgg cagcgacgaa ctcgaggcct
     4441 ggtgccgcga acggctcgcg ggctacaagg tgccgaagga attccgcgcg ctggccgaac
     4501 tgccgcgcaa tgccggtggc aaggtcctca agaaggaact gcgccagttg agttgaacgg
     4561 g
//
LOCUS       DAOQVY010000156         4480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_21635_length_4480_cov_5.522486, whole genome shotgun sequence.
ACCESSION   DAOQVY010000156 DAOQVY010000000
VERSION     DAOQVY010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21635_length_4480_cov_5.522486"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /gene="fliF"
                     /locus_tag="PKN91_11525"
     CDS             complement(<1..1023)
                     /gene="fliF"
                     /locus_tag="PKN91_11525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNS28738.1"
                     /translation="MNAEVVQLPNLGRISAGLRPLLLLVGIAAAVAVGTSIVLWSQRP
                     GLTLLYTGLAAGDLVEVQQSLRAAGIHHEADVERGTISVPAAKLNDARLTLASQGVLQ
                     GSDGFSAIAKDSGFGVSQFMEGARYQHALESELARTIGSLQPVAAARVHIAAPRQSAF
                     VRDRRNATASVFVQLRTGRRLESEQVTAIVNLVASSVPELDPTDVTVVDQSGRLLSQP
                     VDDTDIAAREKQFEFARRLETEYQQRIEALLAPVVGEGRVRAQVVASVDMSVTEEARE
                     QYRPESQVVRSEQLAEESSRSAAGTAGVPGALSNQPPVPGTALAPGVQPSGAQPAAGS
                     TARPARR"
     gene            complement(1049..1405)
                     /gene="fliE"
                     /locus_tag="PKN91_11530"
     CDS             complement(1049..1405)
                     /gene="fliE"
                     /locus_tag="PKN91_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNS28739.1"
                     /translation="MSQMEIDRVLAQIRTLSSQAARPQAPKAGIDAAASAVGGSSRSE
                     PAAFATLMKRGIEQVNATQQNASQLATAFQQGVPGVELPQVMLEMQKASVSFRAATEV
                     RNRLVSVYQEIMNMPI"
     gene            complement(1427..2410)
                     /locus_tag="PKN91_11535"
     CDS             complement(1427..2410)
                     /locus_tag="PKN91_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNS28740.1"
                     /translation="MDRDTSSATPEPVACAQASRETLALARRVATSDCTVLIAGESGT
                     GKEVIARYIHRSSPRAAGAFVAVNCAAIPENMLEAVLFGYEKGAFTGAHAAHAGKFEQ
                     AQGGTLLLDEISEMPLALQAKLLRVLQEREVERIGGRGSVALDVRVLATTNRRLRSEV
                     TAARFREDLYYRLNVFPMVLAPLRERRDDILPLAMHLLSMSCAPGQRIPALAADAAQL
                     LLMHGWPGNVRELDNVMQRALILLDGEVIQAEHVRFDMDEGAEPAAPAVSQGQLSSAL
                     ATAERELILEALRDGHGSRRAAAERLGISPRALRYKLAQLRASGVDLAACG"
     gene            complement(2501..3574)
                     /locus_tag="PKN91_11540"
     CDS             complement(2501..3574)
                     /locus_tag="PKN91_11540"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNS28741.1"
                     /translation="MPTGRSTAVQGVIRLINSVAGHDSSVLILGESGTGKEVVARAIH
                     EASPRRHRPFVAINCGAIPAELLESELFGHEKGAFTGAIATRKGRFEIAEGGTLFLDE
                     IGDMSLSMQVKLLRVLQERVYERVGNHQPMRCNVRIIAATHRNLEESIERGGFRQDLF
                     YRLNVFPIEMPALRERLADLPALLADFTEANARSGRGRVAFTPAAMRALSGYNWPGNI
                     RELANLVERLSILHGGGAVDVQDLPARYRANIPDTALPEVIDEAPISDAEALQVLEGA
                     PGPTSLAELPAEGIDLRGHLDAIERALVQQALDRSSGTVAHAARLLGLRRTTLVEKLR
                     KLGEPALEPAGVLTDKGGCRAVV"
     gene            complement(3774..4343)
                     /locus_tag="PKN91_11545"
     CDS             complement(3774..4343)
                     /locus_tag="PKN91_11545"
                     /inference="COORDINATES: protein motif:HMM:NF028133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNS28742.1"
                     /translation="MDDGIDTIVLDDELAYVDVVPLCWVPLSEPPGAEQTSAQAQHNL
                     RLLQALAALEDSGPNEKPDENSPHSADLMRLEFKVNLLLELVGKILARAQPRPAPVPM
                     RFNAHSAIWRSQDPAPAVGEAGQLEIYLREALVEPLRLFGHVASVGIDRRVKVRFAPL
                     AEPLADLIEKLAFRRHRRQVAGTRQPKNA"
BASE COUNT          675 a         1552 c         1517 g          736 t
ORIGIN      
        1 ccggcgtgcc gggcgcgctg tcgaaccagc cgccggttgg gcgcccgacg gctgaacccc
       61 gggtgccagc gccgtgcccg gcaccggcgg ctggttcgac agcgcgcccg gcacgccggc
      121 ggtgcccgcg gcgctgcgcg aactctcctc ggccaactgc tcgctgcgta ccacctggct
      181 ctccgggcgg tactgctcgc gcgcctcctc ggtgaccgac atgtcgaccg aagcgacgac
      241 ctgcgcgcgc acgcgccctt cgccgaccac gggcgcgagc agcgcctcga tgcgctgctg
      301 gtactcggtc tcgagccggc gcgcgaattc gaactgcttc tcgcgcgctg cgatgtcggt
      361 gtcgtcgacc ggctgcgaca gcaggcgtcc gctctggtcg acgacggtca cgtccgtcgg
      421 gtcgagctcc gggacgctcg atgcgaccag attcacgatc gcggtaacct gctccgactc
      481 gaggcgacgg ccggtgcgca gctgcacgaa gaccgacgca gtcgcattgc ggcgatcgcg
      541 cacgaacgcc gactggcgtg gcgcggcgat gtgcacgcgc gccgcggcga ccggctgcag
      601 gctgccgatg gtacgcgcca gctcggactc gagcgcatgc tggtagcgcg ccccctccat
      661 gaactggctg acaccgaagc ccgagtcctt ggcgatcgcg ctgaatccgt cgctcccctg
      721 cagcacgccc tgcgaggcga gcgtcaggcg cgcatcgttc agcttcgcgg ccggcacgct
      781 gatcgtgccc cgctcgacat cggcctcgtg atggatgccg gcggcgcgca gcgactgctg
      841 cacctcgacg agatcgcctg cggcaagacc cgtgtagagc agcgtcaggc ccgggcgctg
      901 cgaccacagc acgatgctgg tgccgacagc caccgcggcc gcgataccca ccagcagcag
      961 caacggcctc aaccccgcac tgatgcggcc caagttgggc agctgtacaa cctcggcatt
     1021 cattcaggac tcctgtgcga gctgccgttc agatcggcat gttcatgatt tcctggtaga
     1081 cactcaccag gcggttgcgg acttcggtcg cggcgcgaaa cgacacgctc gctttctgca
     1141 tctcgagcat cacctgcggc agctcgacgc ccggcacgcc ctgctggaag gccgtggcga
     1201 gctggctcgc gttctgctgg gtcgcattga cctgctcgat gccgcgcttc atcagggtcg
     1261 cgaaggcagc cggctccgac cgcgacgatc cgcccacggc gctggccgct gcgtcgatgc
     1321 cggctttcgg tgcctgcggg cgcgcggcct gcgaggacag cgtgcggatc tgtgcaagga
     1381 ctcgatcgat ttccatctgg ctcatgcgtg ctctccgggg tgcagctcag ccgcacgcgg
     1441 caagatcgac gcccgacgcg cgcagctgcg cgagcttgta gcgcagcgcc cgcggactga
     1501 tgccgaggcg ctcggcggcg gcgcgccggc tgccgtgccc atcgcgcagc gcctcgagga
     1561 tcagctctcg ttcggccgtc gccagcgcgc tcgagagctg cccctggctc actgcggggg
     1621 cagcaggctc ggcgccttcg tccatgtcga agcgcacgtg ttctgcctgg atcacctcgc
     1681 cgtcgagcag aatcagcgcg cgctgcatga cgttatcgag ttcgcggaca ttgcccggcc
     1741 agccatgcat cagcagcaac tgcgccgcgt cggcggcaag cgcgggtatc cgctggcccg
     1801 gcgcacaact catcgacagc aggtgcatcg cgagcggcag gatgtcgtcg cgccgctcgc
     1861 gcagcggcgc gagcaccatc gggaatacat tcaggcggta gtacaggtcc tcgcggaatc
     1921 gcgcggccgt cacctcgctg cgcaggcggc ggttggtggt agccagcacg cgcacgtcga
     1981 gcgcgacaga tccacggccg ccgatgcgct ctacctcgcg ctcctgcagc actcgcagga
     2041 gctttgcctg cagcgcgagc ggcatctcgg agatctcgtc gagcagcagg gtgccgccct
     2101 gggcctgctc gaacttgccc gcgtgcgcgg catgggcgcc ggtgaaggct cccttctcgt
     2161 agccgaacag cacggcttcg agcatgtttt cggggatcgc ggcgcagttc accgcgacga
     2221 acgcaccggc cgcgcgcggc gatgaccgat ggatgtagcg cgcgatgact tccttgccag
     2281 tgccggactc gccggcgatc agcaccgtgc agtcgctcgt cgccacacgg cgggcgagcg
     2341 cgagcgtctc gcgggatgcc tgtgcgcagg cgaccggttc cggcgtcgcg ctgctcgtgt
     2401 ctcgatccat ccagttcgct ccgtcggcaa aatgccgggc gtcactggta tggagcaaat
     2461 tccgtgccgg gccgattccg cgacggaacc gtgaggcaca tcacacgacg gcgcggcagc
     2521 cgcccttgtc cgtcaaaact ccggcaggtt ccagcgccgg ttcgccgagc ttgcgcagct
     2581 tctcgacgag cgtcgtgcga cgcaggccga gcaggcgggc ggcatgcgcc accgtgccac
     2641 tggaacgatc gagcgcctgc tgcacgagcg cgcgctcgat cgcgtcgaga tggccgcgca
     2701 ggtcgatgcc ttcggccggc agttcggcga gcgaggtcgg cccgggggca ccctccagga
     2761 cctgcaatgc ctcggcatcg gagatcggcg cctcatcgat cacctcgggc agcgcggtat
     2821 ccgggatgtt cgcgcggtag cgcgccggca gatcctgcac atcgacagct ccgccaccgt
     2881 gcaggatcga caggcgctcg acgaggttgg cgagttcgcg gatgttgccg ggccagttat
     2941 agcccgacag cgcccgcatc gcggccggcg tgaaggccac gcgaccgcga ccgctgcgcg
     3001 cgttcgcttc ggtgaaatcc gcgagcagcg ccggcagatc ggcgaggcgc tcgcgcagtg
     3061 cgggcatctc gatcggaaag acattgaggc ggtagaagag gtcctggcgg aagccgccgc
     3121 gctcgatcga ttcctcgagg ttgcgatggg tggcggcgat gatgcgcaca ttgcagcgca
     3181 tcggctgatg gttgcccacg cgctcgtaga cccgttcctg cagcacgcgc agcagtttca
     3241 cctgcatcga caggctcatg tcgccaattt cgtcgaggaa cagcgtgccg ccttcggcga
     3301 tctcgaaacg gcccttgcgc gtcgcgatcg caccggtgaa ggcgcccttt tcgtggccga
     3361 aaagttcgct ctcgagcagc tcggcgggaa tggctccgca gttgatggcg acgaacggac
     3421 ggtggcggcg gggactcgct tcgtggatgg cgcgcgccac cacttccttg ccggtgcccg
     3481 actcgcccag gatcaggacg ctggaatcat gcccggcgac gctgttgatc agccggatga
     3541 cgccttgcac ggccgtcgat cggccggtcg gcatcactgg ttcgtcgtta ttcgccctgg
     3601 cttcactgcc aaaacccaca tccctgtgaa tgccatctgc gttgagagac atccacccag
     3661 ccccgtcgtt ttattgcggg ctattctggc ggcccgttcc ccgagctggt gtgacacggt
     3721 tcaatcgacg cggccacgac cgtggtattg cgtcgcaata tgaaaagtct gcgtcaggcg
     3781 ttcttcggct ggcgcgtacc ggccacctgg cgccgatggc gacggaacgc gagcttctcg
     3841 atcagatcgg cgagcggctc ggcgagcggc gcgaaacgca ctttcaccct ccgatcgata
     3901 ccgaccgacg ccacgtggcc gaacaggcgc aacggttcga ccagggcctc gcgcaggtag
     3961 atctcgagct ggccagcctc gcccaccgcc ggcgcgggat cctgtgaccg ccagatggcc
     4021 gaatgcgcat tgaagcgcat cggcacgggt gccgggcgcg gctgtgccct ggccaggatc
     4081 ttgccgacga gttcgagcag cagattgacc ttgaactcga ggcgcatcag gtccgcggaa
     4141 tgcggcgagt tctcgtcggg cttttcgttc ggtccggagt cctcgagtgc cgcgagggcc
     4201 tgcagcaggc gcaggttgtg ctgggcctgt gccgaggtct gctcggcgcc cgggggctcg
     4261 ctgagcggca cccagcacaa cggcacgacg tcgacgtacg cgagttcgtc atcgagcacg
     4321 atcgtgtcga tgccgtcgtc catcagcggg tctgccctgc gcggcgcgct tcggccggca
     4381 gcattaccca cgcgctgcgg atctcggtca gcagccgaag cacctcgtcg agaatctccg
     4441 ttcggccctc ggcgtttgcc ttggcgagct gctgcaccat
//
LOCUS       DAOQVY010000157         4304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_22873_length_4304_cov_5.010120, whole genome shotgun sequence.
ACCESSION   DAOQVY010000157 DAOQVY010000000
VERSION     DAOQVY010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22873_length_4304_cov_5.010120"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="PKN91_11550"
     CDS             complement(<1..1228)
                     /locus_tag="PKN91_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28743.1"
                     /translation="MRPAASPGLRSRLAGPLAQAAVSALLVFALLAAFLLFSYRSTEA
                     TITTASRNEVRVLATQLGGALRRIEMSVDFVHDKYGRLGLHENTGSPAWKDSLARLQL
                     DMAQLGHHFPEVRSMLIADREGQVQASNLSETPQWNISDRAYFQQAREQATDRLQFSE
                     PLLDKGDARPIVIAYRSILDPDGRFAGLVAVALDLQRLQALLAGVDTGDRGMVSVSRS
                     DDSRLILRWPAAAAKVNQTASEAPSFQRIQAGETSGVARYVGVVDGVERIFAFHRLEP
                     YPFFVLIGRAVDEQFALWWRTGSLASLIAVATLALLLAMQAALHRSRRQLARNKRDFD
                     ALVESRQEATCAWLPDTTLLTCNERYAELLGKAAASLPGTRWIDGLPPHLREQTASAI
                     AQMLQGAGPLTTDRRIR"
     gene            1536..3662
                     /locus_tag="PKN91_11555"
     CDS             1536..3662
                     /locus_tag="PKN91_11555"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNS28744.1"
                     /translation="MLSLRPLFAAIVRLLDRFDYPSKFGAVAIVFGFAVSLYLQQVFN
                     DYGRTVAAVKREIAALEVVDDGLALMVDAQLHRGLSYAALQPAGSGLAAMLPVRAAVV
                     RARMAELDGALGTVSGLEVVSPRWERIRGDLEEALLAADGRATAEHVFERYTVAIRGL
                     LEWLSDVGDASGLGADGDPALHHLAAAQLRSLPTFIETIANLRGMATGSLVASRVDPR
                     TRLGLAARLEAVELAENALRERIERIGAARADVFGLRAPLLRELHDAVRYVRNTARFA
                     LHAEAGSVDPMDFFAMASLALQRASEVHRQHLHRAARELLEQRRQAAVRELWSGVAGS
                     AAVCALVGLLFAAMYASIRRAVAELERGSEHFAAGALDTRVDIVSHDELRTVGERFNA
                     MADKITVLIQGQREQARRLELAASVFASAREGIIITDRKGDIVDVNRAFTKITGWTRD
                     EIVGKNPRVLKSGRQGPDFYRAMWAALAQKGYWEGEVWNRDRQGKEFAEMLAISAVRD
                     SGGEVSHYVALISDITSQKENEQRLRRLAHFDTLTGLPNRTLLADRLEQALGRARRSG
                     KPVAVALIDLDGFKEVNDRLGHDAGDALLVTVARRMKDCLRTQDTIARMGGDEFIAVI
                     GELDDHDEVRRPLERLLAVLAEPIELEQGTVRVAGSIGVTFYPQAGPIESEQLIRQAD
                     QAMYAVKQAGKNGYRIFTDSMPSMRP"
     gene            complement(3631..>4304)
                     /locus_tag="PKN91_11560"
     CDS             complement(3631..>4304)
                     /locus_tag="PKN91_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNS28745.1"
                     /translation="IGRWVLETACMQLARWAAEPARAGLPISVNVSARQILDDRFVDD
                     VLEVLARTGADPGRLKLELTESSLLVDVEAVIGKMNMLKARGIGFSLDDFGTGYSSLA
                     YLKRLPLEQLKIDQSFVRDILDDANDAEIARLVIMLAASLGLVVVAEGVETEAQRENL
                     AGLGCERYQGYLFARPLPIAELEAFLAERDTVAAVAAGSCAGPEVARAGGRPALRAAS
                     TASSR"
BASE COUNT          643 a         1484 c         1517 g          660 t
ORIGIN      
        1 ggcggatgcg gcggtcggtg gtgagcgggc ccgcgccctg cagcatctgg gcgatcgcgc
       61 tcgccgtctg ttcgcgcagg tgcggcggca ggccgtcgat ccagcgcgtg cccggcaggg
      121 atgcggccgc cttgccgagc agttcggcgt agcgttcgtt gcaggtcagc agcgtggtgt
      181 ccggcagcca cgcacaggtc gcttcctggc ggctctcgac cagggcgtcg aagtcgcgct
      241 tgttgcgtgc gagctggcgg cggctgcggt gcagggcggc ctgcatcgcc agcagcagcg
      301 ccagggtggc gacggcgatg aggcttgcga gcgaaccggt ccgccaccac agcgcgaatt
      361 gctcgtccac cgcacggccg atgaggacga agaagggata gggttcgagt cgatggaacg
      421 cgaagatgcg ctcgacccca tccaccacgc ccacgtaacg cgccaccccg cttgtttctc
      481 ctgcctgtat gcgctggaag gacggggcct cggacgccgt ctggttgacc ttggcggccg
      541 ccgcgggcca gcgcaggatc agcctgctgt cgtcgctgcg gctcacgctg accatgccgc
      601 ggtcgcccgt atcgacgccg gcgagcaggg cctgcaatcg ttgcaggtcg agcgcgacgg
      661 cgacgaggcc ggcgaagcgg ccgtccggat ccaggatcga ccggtatgcg atcacgatcg
      721 gtcttgcgtc acccttgtcg agcagcggct cggagaactg cagcctgtct gtggcctgtt
      781 cgcgcgcctg ctggaaatag gcgcgatcac tgatgttcca ttgcggcgtc tcgctcaggt
      841 tgctcgcctg cacctggccc tcccgatcgg cgatcagcat tgagcggacc tccgggaagt
      901 gatgcccgag ctgcgccatg tcgagctgca ggcgagcgag ggagtccttc caggccggcg
      961 agccggtgtt ttcgtggaga ccgaggcggc cgtacttgtc gtggacgaag tcgacgctca
     1021 tctcgatccg gcgcagcgcg ccaccgagtt gggtcgcgag gacccgcacc tcgttgcgcg
     1081 aggcggtggt gatcgtggct tcggtcgagc gatacgaaaa cagcaggaag gctgcgagca
     1141 gggcgaaaac gagcagcgcc gacaccgcgg cctgcgccag cggccccgcg agacggctcc
     1201 gcaagcccgg acttgcagcg ggtctcatgc gcgcgcccca cgcacgatga atggcggacg
     1261 cggtcgatcg gcgcgggtag gtcggagcat cgggaaacgc gtcgcggggg agtcgaagaa
     1321 cctcggcatt atccgcgctt cgcgcgcgcc cttgctggac aaaaatcacg aaggcatgca
     1381 ggcggcggtc gcgggcggcc gcgaccgcat gccgtcgatt cgtcgtgcga gcgtgatccc
     1441 catcgcaggt acgagggcat gcgaggggct aacattcgcg ctttatctcg ccgaaggcgc
     1501 tccactcggc atggcaagcc gccatgcctc cctcgatgct ctcgctacgc cccctcttcg
     1561 ccgcgatcgt gcgcctgctc gatcggttcg actacccgag caagttcggg gcggtcgcga
     1621 ttgtgttcgg cttcgccgtg tcgctgtacc tgcagcaggt cttcaacgat tacggccgta
     1681 ccgtggctgc ggtcaagcgc gagatcgccg cgctcgaagt cgtcgatgac ggtttggcgc
     1741 tgatggtcga cgcccagttg catcgcggcc tcagctacgc cgcgctgcag cctgcaggca
     1801 gcggcctcgc ggccatgctg ccggtgcgtg cggcggtcgt gcgtgcgcgg atggccgagc
     1861 tggacggcgc gctcggcacg gtgtccgggc tggaggtcgt cagcccgcgc tgggagagga
     1921 ttcgcgggga tctcgaggag gctttgctcg ccgccgacgg ccgggcgacc gccgagcacg
     1981 tcttcgagcg ctacaccgtc gccatccggg ggctgctgga gtggttgagc gatgtcggcg
     2041 acgcgagcgg actgggggcc gatggcgacc cggcactcca ccatctcgcc gcagcgcagc
     2101 tgcgttcctt gccgacgttc atcgagacca tcgccaatct gcgcggcatg gcgaccggca
     2161 gcctggtcgc cagccgggtg gatccgcgca cccggctcgg gctcgccgcg cgcctggagg
     2221 ccgtcgagct cgccgagaac gcgctgcgcg agcgcatcga gcgcatcggc gccgcccgcg
     2281 ccgacgtgtt cggcctgcgc gcgccgctgc tccgcgaact ccacgacgcc gtgcgctatg
     2341 tccgcaacac cgcccgcttc gccctccatg ccgaggccgg gagcgtcgat ccgatggact
     2401 tcttcgcgat ggccagcctg gccctccagc gcgcctccga ggtccatcgc cagcacctgc
     2461 accgcgcggc gcgcgaattg ctcgagcagc gccgccaggc cgccgtgcgc gagctctggt
     2521 ccggcgtggc cggctcggcc gcggtgtgcg ccctggtcgg cctgctgttc gccgcgatgt
     2581 acgcgtcgat ccggcgcgcg gtggccgagc tggagcgggg cagcgagcac ttcgccgcag
     2641 gggcgctcga tacgcgcgtc gacatcgtct cgcacgacga gctgcgcacg gtcggagagc
     2701 gcttcaatgc catggccgac aagatcaccg tgctgatcca gggccagcgc gaacaggccc
     2761 gccggctgga gctcgcggcc agcgtcttcg ccagcgcgcg cgagggcatc atcatcaccg
     2821 accgcaaggg cgacatcgtc gatgtcaatc gcgccttcac caagatcacc ggctggacgc
     2881 gcgacgagat cgtcggcaag aacccgcgcg tgctcaagtc gggccggcag gggccggact
     2941 tctaccgggc gatgtgggcc gccctggcgc agaaggggta ctgggaaggt gaggtgtgga
     3001 accgcgaccg gcagggcaag gagttcgccg agatgctggc gatctccgcg gtacgcgact
     3061 ccgggggcga ggtctcgcac tacgtcgcgc tgatctcgga catcacctcg cagaaggaga
     3121 acgagcagcg cctgcgccgg ctcgcccact tcgacacgct caccgggctt cccaaccgca
     3181 cgctgctggc cgaccggctc gaacaggcgc tcggtcgcgc ccgccgctcc ggcaagccgg
     3241 tcgccgtggc gctgatcgat ctcgacggtt tcaaggaggt caatgaccgc ctcggccacg
     3301 acgccggaga cgccttgctg gtgacggtcg cccgtcgcat gaaggactgc ctgcgcaccc
     3361 aggacacgat cgcgcgcatg ggcggcgacg agttcatcgc ggtgatcggt gaactcgacg
     3421 atcacgacga ggtccgccgc ccgctcgagc gcctgctcgc cgtgctcgcc gagccgatcg
     3481 aactcgagca gggcaccgtc cgggtcgccg gcagcatcgg cgtgaccttc tacccgcagg
     3541 ccgggccgat cgagtccgag cagctcatcc gccaggccga ccaggccatg tatgcggtca
     3601 agcaggcggg caagaacggc taccgcatct tcaccgactc gatgccgtcg atgcggccct
     3661 gagggccggt cgtccgcccg ctcgagcgac ctccgggccg gcgcagctgc cggcggcgac
     3721 ggccgccacg gtatcgcgct ccgcgaggaa ggcctccagt tcggcgatcg gcagcgggcg
     3781 ggcgaacagg tagccctggt aacgctcgca ccccaggccg gcgagatttt cgcgctgggc
     3841 ttcggtttcg accccttcgg cgaccaccac caggcccagg ctcgcggcga gcatgatcac
     3901 cagccgcgcg atctcggcgt cgttggcgtc gtccaggatg tcgcgcacga agctctggtc
     3961 gatcttcaat tgctccaggg gcaggcgctt gagataggcg agcgaggaat agcccgtgcc
     4021 gaaatcgtcg agcgagaagc cgatgccccg cgccttgagc atgttcatct tgccgatcac
     4081 ggcctcgaca tcgaccagca gcgagctctc ggtcagttcg agcttgagcc tgccgggatc
     4141 ggcgcccgtg cgcgccagca cctcgagcac gtcgtcgacg aagcggtcgt cgaggatctg
     4201 ccgggcgctg acattgaccg agatcggcag gccggcccgg gccggctccg cggcccacct
     4261 cgccagctgc atgcacgccg tctcgagcac ccagcgtccg atcg
//
LOCUS       DAOQVY010000158         4283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_23026_length_4283_cov_6.198202, whole genome shotgun sequence.
ACCESSION   DAOQVY010000158 DAOQVY010000000
VERSION     DAOQVY010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23026_length_4283_cov_6.198202"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(307..383)
                     /locus_tag="PKN91_11565"
     tRNA            complement(307..383)
                     /locus_tag="PKN91_11565"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(347..349),aa:Met,seq:cat)
     gene            complement(454..1953)
                     /locus_tag="PKN91_11570"
     CDS             complement(454..1953)
                     /locus_tag="PKN91_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNS28746.1"
                     /translation="MDVIRRREFVGGAVGTGVLMAAAPRVLAAQKLDDVDAQIARGHD
                     AAVLRLQDWIRQITIAAENRGIDEGCSYMMQLARDAGFQHVERVPTRGHPSVFATLDA
                     GARRTLGVYFMYDVKQVDPAEWSSPPWEAQLVDVPGAGKAIMGRGAVNQKGPQASLLS
                     ALHAIRAAGRKPPVNLVLIAESEEEIGSPNFADAVLAPQVRAALEKCIGIFMPEAAQS
                     LDGTVQVSLGAKGVVELELVASGAKWGRGPSQDVHSSNRARLDSPAFHLVQALGTLVD
                     DKGDPAIDDWWDDVRPLSAAEERMLDVAATRMSEASAKQSMGVTRWARDADWRQALED
                     MAARPTVNIEGLVGGYTGEGGKTVLPHRAVAKLDLRLVPDMTRDSAVAKLRSHLARHG
                     YGDIEVNVSGGYDPTTTPADAALIRTMMRVYEAEGIDPIFWPRRGGSWPGFVFTSEPL
                     KLPAGHFGLGHGQRAHAPDEYYLVESSNPKLAGIDGAVRSFVQFLYALA"
     gene            complement(1987..3435)
                     /gene="nuoN"
                     /locus_tag="PKN91_11575"
     CDS             complement(1987..3435)
                     /gene="nuoN"
                     /locus_tag="PKN91_11575"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HNS28747.1"
                     /translation="MPDTQTAWSTISAAAPEMYLVAAICAVLLIDVFAGGAKRPRLTS
                     TITLVALAVGAWVTLDYADVSVRVLAFDGLYVADPLALVLKLSGLLFTAVALLYSRPY
                     LERRGIAGGEYYVLALTALLGIFVLASANHLLTLYLGVELLALSLYAMVAFDRDSGVA
                     AEAAMKYFVLGSIASGMLLYGISVIYGLTGTLAIDVLAQQLAADPSLGLIVGTAFVIV
                     AIGFKFGAVPFHMWVPDVYHGAPTAVTLFVSTAPKFAYFALAYRFLSQGLDGISPVWT
                     QMVAVIAVLSLFVGNVFAIAQSNLKRMLAYSAIANVGFILLGFATGTAAGYRAALYYT
                     IVYVLMTLGSFGVILLAGRRGHEADELEDYKGLHSRDPMLALMMLMLMFSTAGVPPFV
                     GFWAKLAIFEALWQVGATWLIVIGALASVVGAFYYIRIVKLMYFDDAPAQPRGEAGFA
                     LRLVLGLNALAALGLGLMPASLMEVCARALAG"
     gene            complement(3448..>4283)
                     /locus_tag="PKN91_11580"
     CDS             complement(3448..>4283)
                     /locus_tag="PKN91_11580"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNS28748.1"
                     /translation="TWLPDAHVEAPTGGSVILAAIMLKMGGYGFLRFSLPITPDASRE
                     LDWLMIGLSLIAIVYISFVALVQTDMKKLIAYSSIAHMGFVTLGSFIAWDIAASTGTL
                     HGAAMGLDGAMVQMISHGLVSGAMFLCVGVMYDRVHSRDIAAYGGVINTMPKFAGFAV
                     LFAFANAGVPGTSGFVGEFLVIIASFKANFWYAFLAATILILGAAYTLWLVKRVIFGP
                     VANDNVARMPDLNGRELLVLGSLAIGVLLLGLWPALLLEIMNSSIHHLVAQMTTSKLP
                     I"
BASE COUNT          771 a         1431 c         1462 g          619 t
ORIGIN      
        1 tgcacccggc gatggcaagg cgctaccggg aggaaatcgc ggcgctgcgg gacgcgctcg
       61 ccaaaaccgg agggcgcggc gaaccggcgg agcatgtgcg ggggctgatc gagaaaatcg
      121 tcctcacgcc ctggtcccat ttggggattg cagggcggga cgaattgaag atcgacttat
      181 ttggcgatct ggccggaatc ctcgacctgg ccgccggagc agaggcggcc agaaaggatc
      241 ggcttatatc cgggaggctc ccaaaagcgg ttaacgataa ccgtctggaa ccctccaaga
      301 tggcgttggt agcgggggca ggatttgaac ctgcgacctt cgggttatga gcccgacgag
      361 ctgccagact gctccacccc gcaccagttg ggccgcgcat cttagcgggg cggccgcggc
      421 attacaaccc gtgctgcgcc tcctgctgcg ggctcaggcg agcgcgtaca ggaactgcac
      481 gaacgagcgg accgcgccgt cgattccggc aagtttcgga ttcgacgatt cgaccagata
      541 gtactcgtcg ggcgcgtggg cgcgctggcc gtggcccagg ccgaaatgcc ccgcgggcag
      601 cttcagcggc tcgctggtga atacgaagcc gggccaggag ccgccgcggc gcggccagaa
      661 gatggggtcg atgccctcgg cctcgtacac gcgcatcatg gtgcggatca gcgcggcgtc
      721 ggccggggtc gtggtcgggt cgtagccacc gctcacgttc acttcgatgt cgccgtagcc
      781 gtgcctggcg aggtggctgc gcagcttcgc cacggcgctg tcgcgcgtca tgtcgggcac
      841 gagccgcagg tcgagcttgg cgacggcgcg atgcggcagc acggtcttgc cgccctcgcc
      901 agtgtagccg ccgaccagcc cctcgatgtt gacggtcggg cgggcagcca tatcctcgag
      961 cgcctggcgc cagtcggcgt cacgcgccca gcgcgtcacg cccatcgact gcttcgcgct
     1021 cgcctcgctc atgcgggtgg cggcgacgtc cagcatgcgt tcctcggccg cggacagcgg
     1081 ccgcacatcg tcccaccagt cgtcgatggc cggatcgccc ttgtcgtcga ccagcgtgcc
     1141 gagcgcctgc acgaggtgga atgcggggct gtcgaggcgc gcgcgattgc tcgagtgcac
     1201 gtcctgcgac gggcccctgc cccacttcgc gccgctggca acgagttcga gctcgaccac
     1261 gcccttggcg ccgagcgaca cctgcacggt gccatcgagc gactgcgccg cctcgggcat
     1321 gaagatgccg atgcacttct cgagtgccgc gcgcacctgc ggcgcgagta cggcatccgc
     1381 aaaattcggc gagccgatct cctcctcgct ctcggcgatc agcacgaggt tcaccggcgg
     1441 cttgcgcccc gcggcgcgta tcgcgtgcag ggcggagagg agcgacgcct gcgggccctt
     1501 ctggttcacg gcgcctcgcc ccatgatcgc cttgccggcc ccggggacgt cgacgagctg
     1561 cgcttcccag ggcggcgacg accattcggc cggatccacc tgcttcacgt cgtacatgaa
     1621 atagacgccg agcgtgcgcc tcgcgccggc gtcgagcgtc gcgaataccg acgggtggcc
     1681 ccgtgtgggt acacgctcga catgctggaa gcccgcgtct cgcgcgagct gcatcatgta
     1741 gctgcagcct tcgtcgatgc cgcggttttc ggccgcgatg gtgatctgtc ggatccagtc
     1801 ctgaagtctc aggacggcgg cgtcatggcc gcgcgcgatc tgtgcgtcga catcatcgag
     1861 cttctgcgcc gcaagaacgc gtggcgccgc ggccatcagc acgccggttc ccacggcacc
     1921 gcccacgaac tcccgtcgcc tgatgacatc catgcccgat ctccctgtcc cgccgtgcgg
     1981 ggaatatcag cccgcgagtg cgcgcgcgca gacctccatg agcgaggccg gcatgagccc
     2041 cagccccagc gccgccagcg cattgaggcc cagcacgagt cgcagcgcga agcccgcctc
     2101 gccacgcggc tgggccggcg catcgtcgaa gtacatcagc ttgacgatgc ggatgtagta
     2161 gaaagcgccg accaccgagg cgagcgcgcc gatcacgatc agccaggtcg caccgacctg
     2221 ccacagcgcc tcgaagatcg cgagtttcgc ccagaagccg acgaacggag gcactcccgc
     2281 ggtcgagaac atcagcatca gcatcatcag cgcgagcatt gggtcgcgcg agtgcaggcc
     2341 cttgtagtcc tcgagttcgt cggcttcatg gccccggcgg ccggccagca ggatcacgcc
     2401 gaatgagccg agggtcatca gcacgtagac gatcgtgtag tagagcgcgg cgcggtaacc
     2461 cgccgcggtg ccggtcgcga agccgagcag gatgaagccg acgttcgcga tggccgaata
     2521 ggccagcatg cgcttcaggt tcgactgcgc gatcgcgaac acgttgccga cgaacagcga
     2581 caggaccgcg atcacggcga ccatctgtgt ccagaccggc gagataccgt cgagcccctg
     2641 cgacaggaac cggtaggcaa gcgcgaaata cgcgaacttc ggcgccgtgg acacgaacag
     2701 ggtcaccgca gtgggcgcgc cgtggtacac gtcgggcacc cacatgtgga acggcacggc
     2761 cccgaacttg aagccgattg cgacgatcac gaaggcggtg ccgacgatca ggccgaggct
     2821 gggatcggcc gccagctgct gcgcgagcac atcgatcgcc agcgtgccgg tcagcccgta
     2881 gatgaccgag atgccgtaga gcagcatgcc ggaggcgatc gagccgagca cgaagtactt
     2941 catcgcggcc tcggcggcga cgccggaatc gcgatcgaag gccaccatcg catagaggga
     3001 cagggcgagc agttcgacgc cgagatacag ggtaagcaga tgattggccg aggcgagcac
     3061 gaagatgccg agcaacgcgg tcagggcgag cacgtagtac tcgccgccgg cgatgccgcg
     3121 gcgctcgaga taggggcgcg aatacagcag cgcgacggcc gtaaagagca ggcccgagag
     3181 cttcagcacc agcgcgagcg ggtcggccac gtagagcccg tcgaacgcca gcacccgcac
     3241 gctgacatcg gcgtagtcga gtgtcaccca ggcgccgacg gcgagcgcca ccagcgtgat
     3301 cgtcgaggtg agccgcggcc gcttcgcgcc gccggcgaac acgtcgatca gcagcacggc
     3361 acagatcgcc gccaccaggt acatctccgg tgcggcggct gagatcgtcg accaggcggt
     3421 ctgcgtgtcg ggcatcgtgc tacggcctca gatcggcagc ttgctcgtcg tcatctgcgc
     3481 gacgaggtgg tgaatggaac tgttcatgat ctcgagcagc agcgcgggcc acaggccgag
     3541 cagcagcacg ccgatcgcca gcgagccgag cacgaggagc tcgcgcccgt tcaggtcagg
     3601 catgcgcgcg acgttgtcgt tggccaccgg gccgaagatc acccgcttca ccagccacag
     3661 cgtgtaggcg gcgcccagga tcaggatggt ggcggccagg aaggcgtacc agaagttcgc
     3721 cttgaagctc gcgatgatca ccaggaactc gccgacgaag ccggaagtgc ccggcacacc
     3781 cgcgttcgcg aacgcgaaca gcacggcgaa gccggcgaac ttcggcatcg tgttgatcac
     3841 gccgccgtag gccgcgatgt cgcggctgtg cacgcggtcg tacatgaccc cgacgcacag
     3901 gaacatggct cccgagacca ggccgtggga gatcatctgc accatggccc cgtcgagacc
     3961 catcgcggcg ccgtgcagcg tgccggtcga cgcggcgatg tcccaggcga tgaacgagcc
     4021 gagcgtgacg aagcccatgt gggcgatcga cgagtacgcg atcagcttct tcatgtcggt
     4081 ctgcacgagc gcgacgaagc tgatgtacac gatcgcgatc agcgacaggc cgatcatcag
     4141 ccagtcgagc tcgcggctgg catcgggcgt gatcggcagg ctgaagcgca ggaaaccgta
     4201 gccgcccatc ttcagcatga tcgcggccag gatcaccgag ccgccggtcg gcgcctcgac
     4261 gtgcgcgtcc ggcaaccagg tgt
//
LOCUS       DAOQVY010000159         4179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_23829_length_4179_cov_5.674345, whole genome shotgun sequence.
ACCESSION   DAOQVY010000159 DAOQVY010000000
VERSION     DAOQVY010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23829_length_4179_cov_5.674345"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PKN91_11585"
     CDS             <1..351
                     /locus_tag="PKN91_11585"
                     /inference="COORDINATES: protein motif:HMM:NF015150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HNS28749.1"
                     /translation="ITLLSGADEPGLEIKTRDGHWLAVNPLAGSIVCNVGDMLQRLTN
                     GVLRSTTHRVANPPATHAGRARYSLAYFLHFNPDFEIRTLPSCISADMPDRYPESITA
                     DAYLQQRLKEIKLL"
     gene            complement(367..1263)
                     /locus_tag="PKN91_11590"
     CDS             complement(367..1263)
                     /locus_tag="PKN91_11590"
                     /inference="COORDINATES: protein motif:HMM:NF015709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HNS28750.1"
                     /translation="MKRAIDVTRAIGAAFIVAGLTGLAGCATVPGARDDDGAQLWRGT
                     LDAVLWMQSSEEYRALAAQVYAQAMSRLPALIEPGTAALEQADAPAQALAALPTAIVL
                     DVDETVLDNSYHQARLLLEGRVYAPQSWDAWVREAVAPPIPGALEFLTAAKRAGHRIF
                     YVTNRECPPAPAPVGEDPCPQRTATMRNLATHGFPDADRVDSFLLLNGKPAWHGSDKG
                     PRRAALASEYRIVALFGDDLRDFVDRGQFAARRSELAPLFGKRWFLLPNPAYGSWERA
                     LVGADCTGTPLECKYSALKRAP"
     gene            complement(1260..3110)
                     /gene="cysN"
                     /locus_tag="PKN91_11595"
     CDS             complement(1260..3110)
                     /gene="cysN"
                     /locus_tag="PKN91_11595"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HNS28751.1"
                     /translation="MGASADLLRFITCGSVDDGKSTLIGRLLYETDSLRADQLASLEA
                     DSKRSGRQAGEPDFALLLDGLAAEREQAITIDVAYRYFATARRRFIVADTPGHEQYTR
                     NMVTGASTADAAVLLIDAQLGLLTQTQRHSCLVSMLGVRHVVIAVNKMDLVGWSRDAF
                     ETIAAQFRAFAGPLGFTTIACVPLSALRGDNVAARSDVAGWYEGPTLLEWLETVDVDA
                     DRSGKPFRMPVQWVNRPDSSFRGFAGTIASGVVRRGDTVVSLPSGAESRIARIVTRDG
                     DLDAAVAGQAVTLTLASAIDVSRGDMLVHAADRPVVADQVEATIVWMHEAPMLPGRAY
                     SVRVGTRTVTGTVSDLKCRIDVATSGQLAARTLELNDIGICTLSFDQPVAFDPYAANR
                     DTGGMVLIDRLTHETIGAGTLRFALRRSQNVHWQFLDVDKRQRAAQKAQQPRVLWFTG
                     LSGAGKSTIANLVERKLHALGRHTYLLDGDNVRHGLNRDLGFRDADRVENIRRVAEVA
                     RLMTDAGLIVLVSFISPFRSERRMARELFAQGEFVEIFVDTALQDAERRDPKGLYAKA
                     RRGELRNFTGIDSPYEVPEHPELRIDTATTSAEDAAELIVAWLGGTREAQ"
     gene            complement(3110..4021)
                     /gene="cysD"
                     /locus_tag="PKN91_11600"
     CDS             complement(3110..4021)
                     /gene="cysD"
                     /locus_tag="PKN91_11600"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004752346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNS28752.1"
                     /translation="MTALPRLTHLDRLEAESLYILREVVAACSRPVLLYSIGKDSSVL
                     LHLALKAFHPARPPLPLLHVDTTWKFREMIAFRDATARRLGLDLLVHTNPDGLRGHID
                     PFVHGPAVYTDVMKTQALRQALDAQGFDAAIGGARRDEELSRAKERIFSLRSAQHRWD
                     PKRQRPELWNLYNTQLGAGETLRVFPLSNWTELDVWQYIERERIEVVPLYFAAERPVV
                     ERGGQLIMVDDERLPLAAGERPKLRRVRFRTLGCYPLTAAIESTASTVAEVVQEMRTM
                     RYSERRGRLIDHDGTASMERKKREGYF"
     gene            3995..>4179
                     /locus_tag="PKN91_11605"
     CDS             3995..>4179
                     /locus_tag="PKN91_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28753.1"
                     /translation="MREARQRGHECRLPPHTTVETARIRPPPDVAEAAAFAEVARGIA
                     IVDEKLITILDVDGVFPA"
BASE COUNT          665 a         1474 c         1402 g          638 t
ORIGIN      
        1 atcacgttgc tgtccggtgc cgacgaaccc ggcctcgaga tcaagacgcg cgacggccac
       61 tggctcgccg tcaacccgct ggccggttcc atcgtctgca acgtcggcga catgctgcag
      121 cggctgacga acggcgtgct gcgctcgacc acgcaccgcg tcgccaatcc gccggcgacc
      181 cacgccggcc gcgcacgcta ttcgctggcc tatttcctgc acttcaatcc ggacttcgag
      241 atccgcacgc tgccgtcctg catatcggcc gacatgcccg accggtatcc cgaatcgatc
      301 accgccgacg cctacctgca gcagcgcctg aaggagatca agctgctctg aacctggcgc
      361 agcgcctcag ggtgcgcgct tcagcgccga gtacttgcac tcgagcggtg tacccgtgca
      421 atcggcaccg accagtgcgc gctcccagga gccataggcc ggattgggca gcaggaacca
      481 gcgcttgccg aacagcggcg cgagttcgct gcggcgagcc gcgaactggc cgcgatcgac
      541 gaagtcgcgc agatcgtcac cgaacagcgc cacgatgcgg tactcgctcg ccagcgctgc
      601 gcggcgcgga cccttgtcgc tgccgtgcca ggcgggtttg ccgttcagca ggaggaagct
      661 gtcgaccctg tcggcgtcgg gaaaaccgtg ggtcgcgaga ttgcgcatgg tcgccgtacg
      721 ctgcgggcac ggatcctcgc cgacgggcgc gggcgccggc gggcattcgc ggttggtgac
      781 gtagaaaatg cggtggccgg cgcgctttgc cgcggtcagg aactcgagcg cccccgggat
      841 cggtggtgcg accgcctccc gcacccacgc gtcccagctc tgcggcgcgt ataccctgcc
      901 ctcgagcagc aggcgcgcct gatggtacga attgtcgagc acggtctcgt cgacatccag
      961 cacgatcgcc gtcggcagcg ccgcgagggc ctgcgccggc gcgtccgcct gttcgagcgc
     1021 ggccgtgccg ggttcgatca gcgcgggcag gcggctcatc gcctgtgcgt acacctgcgc
     1081 ggcgagtgcg cggtactcct ccgacgactg catccacagc acggcatcca gcgtcccgcg
     1141 ccacagctgc gcgccatcgt cgtcacgggc tcccggcacg gtggcgcagc ccgcgaggcc
     1201 ggtcaggccc gcgacgatga acgccgcacc gatcgcccgc gtcacgtcga tcgcccgttt
     1261 cactgcgcct cccgcgtgcc gccgagccag gccacgatca gctccgccgc atcctccgcc
     1321 gacgtcgtcg cggtatcgat gcgcaattca gggtgctccg ggacctcgta gggcgaatcg
     1381 atgccggtga agttgcgcaa ctcgccgcgg cgcgccttgg cgtacagccc cttcgggtcg
     1441 cgccgctcgg catcctgcag cgcggtatcg acgaagatct cgacgaactc accttgcgcg
     1501 aacagctcgc gcgccatgcg ccgctccgag cggaacggcg aaatgaacga caccagtacg
     1561 atcagcccgg catcggtcat cagccgcgcg acctcggcga cacggcggat gttctcgacc
     1621 cggtcggcgt cacgaaagcc gaggtcacga ttgaggccgt ggcgcacgtt gtccccgtcc
     1681 agcaggtaag tgtggcgacc gagcgcgtgc agcttgcgct cgaccaggtt ggcgatcgtg
     1741 gacttgccgg cgcccgaaag cccggtgaac cacagtacgc gcggctgctg ggccttctgc
     1801 gcggcgcgct gccgcttgtc gacgtcgaga aactgccagt gcacgttctg cgagcggcgc
     1861 agcgcgaagc gcagtgtacc ggcgccgatg gtctcgtgcg tcagccggtc gatcagcacc
     1921 atgccgccgg tgtcgcgatt ggcggcgtaa gggtcgaacg cgaccggctg gtcgaacgag
     1981 agcgtgcaga tgccgatgtc gttgagctcg agcgtgcgcg cggcgagctg cccggatgtc
     2041 gcgacgtcga tgcggcactt gaggtcggag accgtgcccg tgaccgtgcg tgttccgacc
     2101 cgcaccgaat aggcgcgacc aggcagcatc ggcgcctcgt gcatccacac gatcgtcgcc
     2161 tcgacctggt ccgcgacgac cggccggtcc gccgcgtgga ccagcatgtc gccgcgactc
     2221 acgtcgatcg cactcgccag ggtgagcgtg accgcctggc cggcgaccgc cgcgtcgagg
     2281 tcaccgtcac gcgtcacgat gcgcgcgatg cgcgactcgg caccggaggg cagcgagacc
     2341 acagtgtcgc cgcgacgcac gacaccgctc gcgatcgtgc ccgcaaagcc gcggaacgag
     2401 gagtccggcc ggttcaccca ttgcactggc atgcgaaacg gcttgccgct gcggtccgcg
     2461 tcgacgtcga ccgtctccag ccattcgagc agcgtgggtc cctcgtacca gccggccacg
     2521 tcactgcgcg cggccacatt gtccccgcgc aatgccgaga gcggcacgca ggcaatggtg
     2581 gtgaagccga gcggtccggc gaaagcgcga aactgcgccg cgatggtctc gaaggcgtca
     2641 cgcgaccagc ccacgagatc catcttgttg acggcgatca ccacatggcg gaccccgagc
     2701 atcgacacga ggcagctgtg ccgctgggtc tgcgtcagca gtccgagctg cgcgtcgatc
     2761 agcagcacgg ccgcatcggc ggtgctggcg ccggtcacca tgttgcgcgt gtactgctcg
     2821 tgtcccggcg tatcggcgac gatgaatctg cgccgcgcag tggcgaagta gcggtacgcg
     2881 acgtcgatcg tgatcgcctg ttcgcgctcg gcggcgagcc cgtcgagcag cagcgcgaag
     2941 tcgggttcgc ccgcctgcct gcccgagcgc ttcgaatcgg cctcgagact cgcgagctgg
     3001 tcggcgcgga gcgagtccgt ctcgtagagc agccggccga tcagtgtgct cttgccatcg
     3061 tctacgctgc cgcaggtgat gaagcgcagc aggtccgcgg aggctcccat cagaaatagc
     3121 cctcgcgctt cttgcgctcc atcgaggccg tcccgtcgtg gtcgatcagc cgcccccgac
     3181 gctccgagta gcgcatcgtg cgcatctcct gcacgacctc agccacagtg ctcgcggtgc
     3241 tctcgatggc ggccgtgagc ggatagcagc ccagcgtgcg aaaccgcacg cggcgcagct
     3301 tcggtcgctc gcccgccgca agcggcagcc gctcgtcgtc gaccatgatc agttggccac
     3361 cgcgctcgac cacgggtcgc tcggccgcga aatacagcgg cacgacctcg atgcgctcgc
     3421 gctcgatgta ctgccagacg tcgagttcgg tccagttcga cagcggaaag acgcgcagag
     3481 tctcgcccgc gcccagctgc gtgttgtaga ggttccacaa ttcggggcgc tggcgcttcg
     3541 gatcccagcg atgctgcgcc gaacgcagtg aaaaaatgcg ctccttggcg cgggacaatt
     3601 cctcgtcgcg ccgcgcgccg ccgatcgccg catcgaagcc ttgcgcgtcg agcgcctggc
     3661 gcagcgcctg ggtcttcatc acgtcggtgt acaccgccgg gccgtgcacg aacggatcga
     3721 tgtgcccgcg caggccgtcc gggttcgtgt gcacgagcag atccagcccc agccggcgcg
     3781 cggtcgcgtc gcgaaaggcg atcatctcgc ggaacttcca ggtcgtatcg acatgcagca
     3841 gcggcagcgg cgggcgcgcc ggatggaacg ccttgagcgc aagatgcagc agcaccgagc
     3901 tgtccttgcc gatcgaatag agcagcaccg gacggctgca cgcggcaacg acttcccgca
     3961 gtatgtacag gctctcggct tcgagtcggt cgagatgcgt gaggcgcggc agcgcggtca
     4021 tgagtgcaga ttaccaccgc acacgaccgt cgagacggcg cggatccgcc cgccgcccga
     4081 cgtggccgag gccgcggcct tcgccgaggt cgcgcggggg atcgcgatcg tcgacgagaa
     4141 gctgatcacg atcctcgacg tcgacggcgt cttcccggc
//
LOCUS       DAOQVY010000160         4099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_24485_length_4099_cov_6.384768, whole genome shotgun sequence.
ACCESSION   DAOQVY010000160 DAOQVY010000000
VERSION     DAOQVY010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24485_length_4099_cov_6.384768"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /gene="murB"
                     /locus_tag="PKN91_11610"
     CDS             <1..679
                     /gene="murB"
                     /locus_tag="PKN91_11610"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNS28754.1"
                     /translation="AVIATNSALGGLERRSAESVYCEAGVACARIAKQCVKWGLGPAE
                     FFAGIPGTLGGALAMNAGAFGGETWNHVVSVEVIDRSGREHTRAAGEYQVGYRSVRPP
                     APDEGFVAALLRFSRRPSMQSHEIRALLEKRKATQPIGEWSGGSTFTNPPGDHAARLI
                     EAAGLKGHRIGGASVSTKHANFIINHGDATAADIEALILHVQAVVEAQAGVRLTPEVR
                     IVGEAKP"
     gene            676..1656
                     /locus_tag="PKN91_11615"
     CDS             676..1656
                     /locus_tag="PKN91_11615"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNS28755.1"
                     /translation="MKLAYHAGRITEVTDAVAFGRVAVMLGGASSEREISLLSGNAVL
                     AALQRCAVDAHAFDPAEQPLADLVSGGFDRVWLALHGPGGEDGTMQGALECLGIPYTG
                     SGVLGSAVCMDKLRTKRLVAAVGVATPESVVLRGPEDFGAALERLGLPLIVKPASQGS
                     SVGMTKVTEAAQLNAAWQAAAVHEPLVFAEPWVTGAEYTVAILKGRALPSIRIETPQV
                     FYDYEAKYFRSDTLYHCPSGLSAGEERRLGELALAACDACGVTGWGRVDFMADSDGQM
                     LFLEINTVPGMTDHSLVPMAARAEEVSFDELVWRVLETSFARTPTMRTRT"
     gene            1656..2453
                     /locus_tag="PKN91_11620"
     CDS             1656..2453
                     /locus_tag="PKN91_11620"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HNS28756.1"
                     /translation="MRLRSRNRRKQTEPAWRLPSIDWRRLAATAGSLAALGGAVAGVA
                     WTLDQPIETISVEGRFQRVSPLDVEQAVKQRVLGTGLVSVDLESVGRSLQSIPWVDSA
                     SVQRAWPRGLRVHVQEQVAAARWGANGLLNTRGELFISESRHIPPELPQLSGPIGTER
                     DVAERYLAVRGRLMEAGLRIAAMRLDERGAWEFDLDNGVTVRLGRRDVDARFERFMVA
                     GLKLVGERGGDITYVDMRYSNGFAIGWRTRGVQLATGKLPADDNPDA"
     gene            2460..3695
                     /gene="ftsA"
                     /locus_tag="PKN91_11625"
     CDS             2460..3695
                     /gene="ftsA"
                     /locus_tag="PKN91_11625"
                     /inference="COORDINATES: protein motif:HMM:NF007009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNS28757.1"
                     /translation="MAKRAERTLIVGLDIGTSKVVALVGEIAADGTIEVIGLGSQPSR
                     GLKKGVVVNIESTVQSIQRAVEEAELMAGCEIHSVFAGIAGSHVRSLNSHGVVAIRDK
                     EVSSHDVVHVIDAAKAVAIPADQKILHVLPQEFVIDGQEGIRDPIGMSGVRLEAKVHI
                     VTGADSAAQNVEKCIQRCGLEVDDVVLEQLASSFATLTDDEKELGVCLVDIGGGTTDL
                     AVFSNGAIRHTAVIPIAGDQVTNDIAVSMRTPTHYAEDIKVRYACALSQLANPDESIE
                     VPSVGDRPARRLARQTLAEIVEPRYEELFSLVRDELRRSGFEEAIAAGIVLTGGSAKM
                     EGAIELAEEVFHVPVRLGLPQQVQGLTDVVRNPIYSTAVGLLLYARDNALPGNRGRTF
                     GGSAKSMLERMKSWFAGNF"
     gene            3733..>4099
                     /locus_tag="PKN91_11630"
     CDS             3733..>4099
                     /locus_tag="PKN91_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010159870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNS28758.1"
                     /translation="MDAYSQSAVIKVLGVGGGGGNAVAHMVGTGIEGVEFLCINTDAQ
                     ALKHSKVKTALQIGANMTKGLGAGADPEVGRQAAMEDRDRIIELIEGCDMLFITAGMG
                     GGTGTGAAPIVAQVAKELGI"
BASE COUNT          664 a         1357 c         1463 g          615 t
ORIGIN      
        1 cgccgtgatc gccaccaaca gcgcgttggg cggcctcgag cgccgctccg ccgagagcgt
       61 ctactgcgag gccggcgtgg cctgcgcgcg catcgccaag cagtgcgtca agtggggact
      121 ggggcccgcc gagttcttcg ccggcattcc gggaacgctc ggcggtgcgc tcgcgatgaa
      181 cgcgggtgcg ttcggcggcg agacctggaa ccacgtcgtc tcggtcgaag tgatcgaccg
      241 cagcggccgc gagcacacgc gtgcggccgg cgagtaccag gtcggctatc gcagcgtgcg
      301 cccaccggcg cccgacgagg gcttcgtcgc ggccctgctg cgcttctcgc gtcggccatc
      361 gatgcagtcg cacgagatcc gcgcgctgct cgagaagcgc aaggcgacgc agccgatcgg
      421 cgagtggagc ggcggctcga cgttcaccaa tccgcccggc gatcacgccg cgcggctgat
      481 cgaggcggcg ggcctcaagg gtcatcgtat cggcggcgca tcggtatcga cgaagcacgc
      541 caatttcatc atcaaccatg gcgatgccac ggctgcggac atcgaggcac tgatcctgca
      601 cgtgcaggcg gtcgtcgagg cgcaggcggg cgtgaggctc acgcccgaag tgcgcatcgt
      661 cggcgaggcc aaaccatgaa actcgcgtat cacgccgggc gcatcaccga ggtgaccgac
      721 gccgtggcct tcggtcgggt ggctgtcatg ctgggcgggg cttcctcgga gcgggagatc
      781 tcgctgctct cgggcaatgc cgtgctcgca gcgctgcagc gatgtgccgt cgatgcgcac
      841 gctttcgatc cggccgagca accgctcgcc gatctcgtga gcgggggttt cgatcgcgtg
      901 tggctcgcgc tgcacgggcc gggcggcgag gacggcacga tgcagggcgc gctcgagtgc
      961 ctaggcatcc cgtacaccgg cagtggcgtg ctcggctcgg cggtgtgcat ggacaagctg
     1021 cgcaccaagc gcctcgtcgc ggcggtcggc gtcgctacgc ccgaaagcgt ggtgctgcgc
     1081 ggaccggagg atttcggcgc agcgctcgag cggctcggcc tgccgctgat cgtgaagcct
     1141 gcctcgcagg gctcgtcggt cggcatgacc aaggttaccg aggccgcgca gctcaacgcc
     1201 gcctggcagg cagccgccgt gcacgagccg ctggtgttcg ccgaaccatg ggtcaccggc
     1261 gccgagtaca ccgtcgcgat cctcaagggg cgcgcactgc cttcgatccg catcgagacg
     1321 ccgcaggtgt tctacgacta cgaggcgaag tactttcgca gcgacacgct gtaccactgc
     1381 ccgtcgggtc tgtccgcggg cgaggagcgt cgcctcggcg aactggcgct cgcggcatgc
     1441 gacgcctgcg gcgtgaccgg ctggggccgg gtggacttca tggccgatag cgacggccag
     1501 atgctgttcc tcgagatcaa caccgtgccg ggcatgaccg accacagcct cgtgccgatg
     1561 gcggcgcgcg ccgaggaggt cagcttcgac gaactcgtgt ggcgtgtgct cgagacgagt
     1621 tttgcgcgca ctccgaccat gaggacgcgg acctgatgcg cctgcgcagc cgcaatcgtc
     1681 gcaagcagac ggagcccgca tggcgcctgc cgtcgatcga ctggcggcgc ctcgcggcga
     1741 cggccgggag cctcgccgcg ctcggcggcg cagtcgcggg cgtggcgtgg acgctcgacc
     1801 agccgatcga gacgatcagc gtcgagggcc ggttccagcg cgtttccccg ctcgatgtcg
     1861 aacaggcggt caagcagcgc gtgctcggca ccggcctcgt cagcgtcgat ctcgagtcgg
     1921 tgggccgcag cctgcagtcg atcccgtggg tcgacagcgc cagtgtgcag cgggcctggc
     1981 cgcgcggcct gcgcgtgcac gtgcaggagc aggtggccgc ggcacgctgg ggcgccaacg
     2041 gcctgctgaa cacgcgcggc gagctgttca tttccgaatc gcgacacatt ccacccgagc
     2101 tgccgcagct ttcggggccg atcggcaccg agcgcgacgt ggcggagcgc tatctcgccg
     2161 tgcgcggccg gctgatggag gcgggtctgc gcatcgccgc gatgcgcctc gacgagcgcg
     2221 gagcgtggga gttcgacctc gacaacggcg tcaccgtgcg gctcggtcgc cgcgacgtcg
     2281 atgcacgctt cgagcgcttc atggtcgcgg gtctgaagct cgtcggcgag cgcggcggcg
     2341 acatcaccta tgtcgacatg cgttattcga acggctttgc gatcggctgg cgcacgcgcg
     2401 gcgtgcaact cgccaccggc aagcttcccg cagacgacaa cccggatgcc tgagcagaca
     2461 tggcgaaacg tgcagaaaga accctgatcg tcggcctcga tatcggcacc tccaaagtgg
     2521 tggcgctggt cggggagatt gccgccgacg gcacgatcga ggtgatcggc ctcggctcgc
     2581 agccctcgcg cggtctgaag aagggcgtgg tcgtcaacat cgagtccacc gtgcaatcga
     2641 tccagcgtgc ggtcgaggag gccgagctga tggccggctg cgagatccac tcggtgttcg
     2701 ccggcatcgc cggcagccac gtgcgcagcc tgaattcgca cggcgtggtg gcaatccgtg
     2761 acaaggaagt gtcgagccac gacgtcgtgc acgtgatcga cgccgccaag gcggtcgcga
     2821 tcccggccga ccagaagatc ctgcacgtgc tgccgcagga gttcgtcatc gacggccagg
     2881 agggcatacg cgacccgatc ggcatgtcgg gcgtgcgcct cgaggcgaag gtgcacatcg
     2941 tcaccggcgc cgacagtgcg gcgcagaacg tcgagaagtg catccagcgc tgcggcctcg
     3001 aagtcgacga cgtcgtgctc gagcagctcg cgtcgagctt cgccacgctc accgacgatg
     3061 aaaaggaact cggcgtgtgc ctcgtcgata tcggcggcgg cacgaccgac ctcgcggtgt
     3121 tctcgaacgg cgcgatccgc cacacggccg tgatcccgat cgcgggcgac caggtgacca
     3181 acgacatcgc cgtgtcgatg cgcacgccga cccactacgc cgaggacatc aaggtgcgct
     3241 atgcctgcgc gctctcgcag ctcgccaatc ccgacgagag catcgaggtg ccgagcgtcg
     3301 gcgatcgccc ggcgcgccgc ctcgcgcgcc agacgctcgc cgaaatcgtc gagccgcgct
     3361 acgaggagct gttcagcctg gtgcgcgacg aactgcgtcg cagcggcttc gaggaagcga
     3421 tcgccgcggg catcgtgctg accggcggca gcgcgaagat ggaaggggcg atcgagctcg
     3481 ccgaggaagt cttccacgtg cccgtgcgcc tcggcctgcc gcagcaggtg cagggcctca
     3541 ccgacgtggt acgcaacccg atttattcga cggccgtggg cctgctgctc tacgcgcgtg
     3601 acaacgcgct gccgggcaat cgcgggcgca cgttcggcgg cagtgcgaag tcgatgctgg
     3661 agaggatgaa gtcctggttt gcaggcaatt tctgagttct atcaaaggag cgtagcgaaa
     3721 atgttcgaat taatggacgc atacagccag agcgccgtga tcaaggtgct cggggtgggc
     3781 ggcggcggcg gcaacgccgt ggcccacatg gtgggcacgg gcatcgaggg cgtggagttc
     3841 ctgtgcatca ataccgatgc ccaggccctc aagcactcga aggtcaagac ggcgctgcag
     3901 atcggcgcca acatgaccaa gggcctcggc gccggcgccg accccgaggt cggtcgccag
     3961 gcggcgatgg aagaccgtga ccgcatcatc gaactcatcg agggctgcga catgctcttc
     4021 atcaccgcgg gcatgggcgg cggcaccggc acgggcgccg cgccgatcgt cgcgcaggtg
     4081 gcgaaggagc tcggcatcc
//
LOCUS       DAOQVY010000161         4027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_25106_length_4027_cov_5.841893, whole genome shotgun sequence.
ACCESSION   DAOQVY010000161 DAOQVY010000000
VERSION     DAOQVY010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25106_length_4027_cov_5.841893"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..608)
                     /locus_tag="PKN91_11635"
     CDS             complement(165..608)
                     /locus_tag="PKN91_11635"
                     /inference="COORDINATES: protein motif:HMM:NF016161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HNS28759.1"
                     /translation="MAEFYPWIKWVHVAAVVASGSLFFVRGLASHLGAGWTMAAPLRY
                     LSYTIDTVLLTAALALTTIVRQYPYMHDWLTVKLLLLIVYIVLGSFALRRGATRGGRI
                     VFWVAALVVLVMIISVARARHPLGLLHDPASLDLHQRALADWPPS"
     gene            complement(633..1373)
                     /locus_tag="PKN91_11640"
     CDS             complement(633..1373)
                     /locus_tag="PKN91_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS28760.1"
                     /translation="MLRPSPPSPIADDGDDFRFCSTCAFSAACLASGYDKSQLRDLHV
                     LVQHVGPFEEGKHIFREGDPFTAIAAVRSGTVKTYVTDANGREQVQGFYLPGEMVGLN
                     AISQSRYPCNAVALEPTVLCRFSFPSMAALATRMPGVQQQLFRLLSQDIGKAALLAGN
                     YTADERMAAFLVSLSRRYAERGFSPTRFLLTMTRTDIANYLRLAAESVSRVFRRFQDE
                     GLIRVDRREIEILQPRTLEDLARSVLRA"
     gene            complement(1343..2542)
                     /locus_tag="PKN91_11645"
     CDS             complement(1343..2542)
                     /locus_tag="PKN91_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28761.1"
                     /translation="MELSRAIDAGLTCSFRRRDAHPARLPAIPRLTTAFDDAAYRMQA
                     GRSNDDPIPRPLAIQLAVHPDPERLIREIEETARHFDRDRDVIALQVDATGAGASRMS
                     DLAALTGSLERHFHFVPAPRRGFVAVLDPLALQPGELAALGALGFDAVEFATDIASTE
                     AALRALESARKAGLRCAVEVNAPPDLAGQDLQVAIDALIAVRPDRLALSLPGEPQGDA
                     CKAVLQAAARLDAAGYSEVGLDPRPLSWLAPAGSQWIAGTLGGGDWAVPPTGADVIGL
                     GIGAMSRIGDCVVESLADADAWAAAIDAGLLPVWRGCVLDTEERLRIDIVEEWLRRGE
                     IPIDAIERRYGIGFAGHFSTALQRLSGYAAEGLASIDSRAVRATSQGRLLLRIMAECF
                     DHHPPRP"
     gene            complement(2586..3188)
                     /locus_tag="PKN91_11650"
     CDS             complement(2586..3188)
                     /locus_tag="PKN91_11650"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNS28762.1"
                     /translation="MHIVGITASAVGSAWLLALALRGGDRGEIAAIAVYCGGLIATFA
                     FSIAYNLTRTPSRQRVLRRFDHAAIYLMIACSYTPFTTQCLQGRWAIGMTSAVWAIAI
                     AGMVAKLGFPTLPRRWSVAGYLVLGWIALVALVPMIETVPRTAMWLIAAGGLVYSLGV
                     PVYVSKDLPLRRAIWHGFVICAAALHWAAVLVGVVTPRVD"
     gene            complement(3288..>4027)
                     /locus_tag="PKN91_11655"
     CDS             complement(3288..>4027)
                     /locus_tag="PKN91_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNS28763.1"
                     /translation="AHAVAPARDRMQRLAALKVLAGRDLRSCVAIDYPPQEAIVRRAK
                     AIRADLVIAATESRTFGKRLLLRNTDWELIRHCPCPLLLIKTPGGYSKPNIVAALDPF
                     HARDKSGKLDSKLLAAANGVAQLLRGRAHAFHAHLPLVATLPGAMGQPVALTLPPDAE
                     DLHRANIRKVFDKLATRGGIPRARRHLRMGDVPTQIEAVLRETKAGIVVMGAVSRSGL
                     QRIFIGSTAEHVLDRVPCDALIIKPGR"
BASE COUNT          667 a         1311 c         1485 g          564 t
ORIGIN      
        1 cgggtgcgcc gaagcgcgca tgcagctgtt cgatcagtac ggtgatgagc accatgtcgc
       61 cgaaggcgcc acagcgcatg agcaggggac gggccatggc gcattctgcg ggccggcggc
      121 ccacgggtcc atggcgccgc ttcagcgcag cgcggcggcg tcgatcacga tggcggccag
      181 tcggcgagcg ctcgctggtg caggtcaagc gacgccgggt cgtgcaacag accgagcggg
      241 tggcgggctc gcgcaacact gatgatcatg accaatacga cgagcgcggc gacccagaag
      301 acgatgcgcc cgccccgggt cgcgccgcgc ctcagcgcga agctgccaag tacgatgtag
      361 acgatgagca gcagcagctt gaccgtgagc cagtcatgca tgtacgggta ctgccgcaca
      421 atggtcgtca gcgcgagcgc cgcggtcagc agcacggtgt cgatcgtgta gctgagatag
      481 cgcagcggcg cggccatcgt ccagccggcg ccgagatggg acgcgaggcc ccggacgaag
      541 aagagcgatc cgctcgcgac caccgcggcg acatgcaccc acttgatcca cggatagaac
      601 tcagccaacg ccgggtccct ggctggaaac ggctacgcgc gcagtacgct gcgggccagg
      661 tcttcgagcg tgcgaggctg caggatctcg atttcccgcc ggtcgacgcg gatcaggccc
      721 tcgtcctgga agcgcctgaa cacccggctc acgctttcgg cggcgaggcg caggtaattg
      781 gcgatgtcgg tgcgggtcat cgtgagcagg aaacgggtcg gcgaaaagcc gcgctcggca
      841 tagcggcgcg acagcgagac gaggaacgcg gccatgcgct cgtctgccgt gtagttgccg
      901 gcgagcaggg ctgccttgcc gatgtcctgg ctcagcagcc ggaacaactg ctgctgcacg
      961 ccgggcatgc gcgtcgcgag cgccgccatc gacggaaacg agaaccggca cagcacggtc
     1021 ggttcgagcg cgacggcatt gcacgggtag cgcgactgcg aaatggcatt gaggccgacc
     1081 atctcgccgg gcagatagaa accctgcacc tgctcgcgtc cgttcgcgtc ggtgacatag
     1141 gtcttgacgg tgccggaacg caccgccgcg atggccgtga acgggtcgcc ctcgcggaag
     1201 atgtgcttgc cttcctcgaa cggaccgacg tgctgcacga gtacgtgcag gtcgcgcagc
     1261 tgcgatttgt cgtagccgct tgcgaggcag gcggccgaga acgcgcaggt gctgcagaag
     1321 cggaaatcgt cgccgtcgtc ggctatgggc gaggggggtg atggtcgaag cactccgcca
     1381 tgatacgcag caacagacgc ccttgcgatg tcgcgcgcac ggcccgcgaa tcgatgctgg
     1441 cgaggccttc cgcggcgtag ccggagagcc gctgcagtgc cgtggagaag tgcccggcga
     1501 acccgattcc gtagcgccgc tcgatggcgt cgatcgggat ttcgcccctg cgcagccatt
     1561 cctcgacgat gtcgatgcgc aggcgctcct cggtatccag gacgcagccg cgccataccg
     1621 gcagcagccc ggcatcgatc gccgccgccc acgcgtcggc atcggcaagg ctctcgacca
     1681 cgcagtcgcc gatgcggctc atggcgccga taccgaggcc gatcacgtcg gcacctgtcg
     1741 gagggacggc ccagtccccg ccgcccaggg tgccggcgat ccattgcgat ccggcaggcg
     1801 cgagccagga gagcggccgg ggatcgagcc cgacctccga gtagccggcc gcgtcgaggc
     1861 gcgccgcggc ctgcagcacc gccttgcatg catcgccctg gggttcccca ggaagcgaca
     1921 gggcgaggcg atcgggccgc acggcgatca gggcatcgat cgcgacctgg aggtcctggc
     1981 ccgcgaggtc gggcggcgcg ttcacttcga ccgcgcagcg caggcctgcc ttgcgtgcgg
     2041 actcgagcgc gcgcaatgcg gcctcggtgc tcgcgatgtc agtggcgaat tcgacggcat
     2101 cgaatccgag cgcgcccagg gcggcgagtt cgccgggctg cagcgccagt ggatcgagca
     2161 ccgccacgaa accgcgcctg ggcgcgggga cgaagtgaaa gtgccgctcg aggctgccgg
     2221 tgagggcggc caggtccgac atgcgagatg ccccggcacc ggtggcgtcg acctgcagcg
     2281 cgatgacgtc gcgatcgcgg tcgaagtggc gcgcagtctc ctcgatctcg cggatcaggc
     2341 gctcgggatc ggggtggacc gccagttgga tggcaagcgg gcgcgggatc ggatcgtcgt
     2401 tgctgcgccc ggcctgcatg cgatacgccg catcgtcgaa tgcggtcgtc aggcgcggta
     2461 tggcgggcag gcgggcgggg tgggcgtcac gccgccgaaa gctgcacgtc aaccctgcgt
     2521 cgatggcgcg ggacagttcc atgcggcatg ttcgccaacg ccgtcgcggc ctgccttgac
     2581 ctggatcagt ccacgcgcgg tgtcaccacg ccgaccagca cggcggccca gtgcaacgcg
     2641 gccgcgcaga tcacgaaacc gtgccagatg gcgcgccgca ggggcaggtc cttcgagacg
     2701 taaacgggca caccgagcga gtacacgagg ccgcccgcgg caatgagcca catggcggtg
     2761 cgcggcaccg tttcgatcat cggaacgagt gccacgagcg cgatccagcc gagcacgagg
     2821 tagccggcga ccgaccagcg gcggggcagg gtgggaaagc ccagcttggc gaccatgccc
     2881 gcgatcgcga tcgcccagac cgcgctcgtc atgccgatgg cccagcggcc ctgcagacat
     2941 tgcgtggtga acggcgtata ggaacaggcg atcatcaggt agatcgccgc gtggtcgaag
     3001 cgtcgcagca cgcgctggcg ggagggcgtg cgggtgaggt tgtaggcgat cgagaacgcg
     3061 aaggtggcga tgaggccgcc gcagtagacg gcgatggcgg cgatctcgcc acgatcgccg
     3121 ccacgcagcg ccagcgcgag cagccaggcc gagccgacgg cgctcgcggt gatgccgacg
     3181 atgtgcaccc acttgtcggc ctggcgttcg gccgcgctac ggtaatgcgg ctcgacgtcc
     3241 tggtgccgga ttttaggtat ttcggctatt gcgtgcggcc gcgccagcta gcggccaggc
     3301 ttgatgatga gcgcatcgca gggcacgcgg tcgaggacgt gctcggcggt gctgccgatg
     3361 aagatgcgct gcaggccaga gcgcgatacc gcgcccatca cgacgatgcc ggccttcgtt
     3421 tcgcgcagga cggcctcgat ctgggtcggc acgtcgccca tgcgcaggtg gcggcgcgcg
     3481 cgcggaatgc cgccgcgcgt ggccagcttg tcgaacacct tgcggatgtt ggcgcggtgc
     3541 aggtcctcgg cgtccggcgg cagcgtgagc gccaccggct ggcccatggc acccggcagg
     3601 gtcgcgacca agggcagatg ggcatggaag gcatgcgcgc ggccgcgcag cagctgcgcc
     3661 accccgttgg cggcggcaag cagcttggag tcgagcttgc cggacttgtc gcgcgcgtgg
     3721 aatggatcga gggcggcgac gatgttcggc ttgctgtagc cgcccggcgt cttgatgagc
     3781 agcaacgggc aggggcagtg gcggatcagt tcccagtcgg tgttccgtag cagcagccgc
     3841 ttgccgaaag tgcgcgattc ggtcgcggcg atcacgaggt cggcccggat cgccttcgcg
     3901 cgacgcacga tcgcctcctg cggcgggtag tcgatggcga cgcaggagcg caggtcccgg
     3961 cccgcgagca ccttgagcgc cgcgaggcgc tgcatgcgat cgcgcgcggg cgccaccgca
     4021 tgggcgc
//
LOCUS       DAOQVY010000162         4014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_25218_length_4014_cov_4.202071, whole genome shotgun sequence.
ACCESSION   DAOQVY010000162 DAOQVY010000000
VERSION     DAOQVY010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25218_length_4014_cov_4.202071"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PKN91_11660"
     CDS             complement(<1..219)
                     /locus_tag="PKN91_11660"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HNS28764.1"
                     /translation="MSRDAESAAVVLLSGGLDSATALAIVRSEGTRCHALSVDYGQRH
                     AAELAAAARIAAHLGAAEHRVMRVDLGGI"
     gene            complement(203..859)
                     /gene="queE"
                     /locus_tag="PKN91_11665"
     CDS             complement(203..859)
                     /gene="queE"
                     /locus_tag="PKN91_11665"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNS28765.1"
                     /translation="MTAAPEARVKITEIFLSVQGEAATVGFPTVFVRLTGCPLRCVYC
                     DTAYAFHGGEWWTLDAVLAGVTAHGVRHVCVTGGEPLAQRNCPALLTQLCDAGFDVSL
                     ETSGAMPIDGVDARVQRVVDIKTPGSGEMHRNRVGDYALLTARDLVKFVITNRDDYEW
                     SRARVAEHGLAARCTVLFSPSYGQMPARELAEWILADRAPVRFQVQLHKVLWGDEPGR
                     "
     gene            complement(856..1650)
                     /gene="ybgF"
                     /locus_tag="PKN91_11670"
     CDS             complement(856..1650)
                     /gene="ybgF"
                     /locus_tag="PKN91_11670"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNS28766.1"
                     /translation="MRHPRLPLAGTLMASIVLGGCSLTPAEEDPVQVRLADLDARVAK
                     VERIIANQSLVELSQRVEALEAHSRELQGRSEVQENAVEGAKQQQRDLYNDLDRRLSA
                     LESGVPEPPREGSGAAASSTVAAAPAAAGEPPSAADQQAAYDAAFAALKDARYADAIT
                     GFRALLEAAPTGDLAPNSQYWLGEAYYVTRDFENAAVAFEAVAERWPDSGKAPGALLK
                     LGYSQYELKRLGDARQTLGRLTARYPDTEAARLAQDRIQRIIAEQR"
     gene            complement(1662..2201)
                     /gene="pal"
                     /locus_tag="PKN91_11675"
     CDS             complement(1662..2201)
                     /gene="pal"
                     /locus_tag="PKN91_11675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HNS28767.1"
                     /translation="MRNLIWVLLAAGALAVGGCASKKGAEKPATDTAQGAGTEAAVGQ
                     GVGDGAVTTAPLGDDTASAGPQGELLSKRIVYFEFDRTELNAQDTAIVNAHARYLAGN
                     PGAKVRLEGHTDERGTREYNIGLGERRSQSVRRAFLLQGVAETQVSTVSYGEERPASA
                     GSDEVAWAQNRRVEIVYLN"
     gene            complement(2225..3544)
                     /gene="tolB"
                     /locus_tag="PKN91_11680"
     CDS             complement(2225..3544)
                     /gene="tolB"
                     /locus_tag="PKN91_11680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HNS28768.1"
                     /translation="MTDARLPARFLFILCMLVGALAATAAHAQLRIEITSGVTDPIPI
                     AIVPFARSVPGDGGIDVAEIVQRDLEGSGRFRAMARRDMIETPSRAADIVVPDWRTSR
                     NDYIVVGRVLPGEGGAAQIEVELVNLVNGQRIAGQRFVAPAGRQREAAHRVSDLIYEK
                     VLGVPGAFATRIAYVAVSGQPPRQTYSLIVADADGEAARTVVESPLPLMSPAWSPDGE
                     WLAYVSFESRSSAIWMQRVSTGERRRVIARAGINGSPAFSPDGKRLAVTLSGSTGNLD
                     IHVLELESGALTRITSDSAIDTEAVFSPDGRTLYFTSDRAGSPQIYRADVLANATAKR
                     VTFTGSYNARGRLSPDGKQLAMVTLAGGAYRVAVQDLATGVVRTLSKGPLDESPSFAP
                     NGAMLIYAGRERGQGTLATIAIDGQTTQRLKADRGEVREPVWGPWRP"
     gene            complement(3537..>4014)
                     /gene="tolA"
                     /locus_tag="PKN91_11685"
     CDS             complement(3537..>4014)
                     /gene="tolA"
                     /locus_tag="PKN91_11685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HNS28769.1"
                     /translation="QAEAERKAEAERQAEAERKAEAERQAEAERQAEAERKAEEARRR
                     AEREAELRRSIAAEERLRAARASGAGAHWRDLIRAKIESEWRRPPTAQPGIECEVQVT
                     QVPGGEVVAVRILACNGDAAVRQSIEIAVMSASPLPAPPDPSLFERNLVVVFKPND"
BASE COUNT          580 a         1445 c         1338 g          651 t
ORIGIN      
        1 gatgccgccg aggtcgaccc gcatgacccg gtgctcggcc gcgccgaggt gggcggcaat
       61 gcgggcggcc gcggccagtt cggcggcgtg gcgctggcca taatcgaccg agagcgcatg
      121 gcagcgcgtg ccttcgctgc gcacgatcgc gagcgccgtg gccgaatcga gcccgccgga
      181 cagcagcacg accgccgcgc tttcagcgtc ccggctcatc gccccacagc accttgtgca
      241 actggacctg gaagcgcacc ggtgcgcggt ccgcgagtat ccactcggcc agttcgcgcg
      301 ccggcatctg gccgtagctc ggcgagaaca gcacggtgca tcgcgcagcg agcccgtgct
      361 cggcgacgcg cgccctgctc cattcataat cgtcgcggtt cgtgatcacg aacttcacga
      421 gatcgcgcgc cgtgagcagc gcgtaatcgc cgacgcggtt gcggtgcatc tccccggaac
      481 ccggcgtctt gatgtcgacc acgcgctgca cgcgcgcatc gacgccgtcg atcggcatgg
      541 cgccgctcgt ctcgagcgac acgtcgaagc ccgcgtcaca cagttgtgtg agcagtgccg
      601 gacagttgcg ctgggcgagc ggctcaccgc ccgtcacgca gacatggcgc acaccatggg
      661 ccgtaacccc ggcgagcacc gcgtccagcg tccaccactc gccaccgtgg aatgcgtagg
      721 cggtatcgca gtacacgcag cgcagcgggc agcccgtcag gcgcacgaac acggtgggaa
      781 acccgaccgt ggcagcctcg ccctgcaccg acaggaaaat ctcggtgatc ttgacccgcg
      841 cctccggcgc tgcggtcacc gctgctcggc gatgattcgc tggatgcgat cctgcgcgag
      901 cctcgccgcc tcggtgtccg ggtagcgcgc ggtgagtcga ccaagggtct gccgcgcgtc
      961 tcccaggcgc ttgagttcgt actgcgagta gccgagcttg agcagggcac ccggcgcctt
     1021 gccggaatcg ggccagcgct cggccacggc ttcgaacgcg accgccgcgt tctcgaaatc
     1081 gcgcgtaacg taataggcct ctccgagcca gtactgcgag ttgggcgcga gatcgccggt
     1141 gggcgccgcc tcgagcagag cgcgaaagcc cgtaatggca tcggcgtatc gggcgtcctt
     1201 gagcgccgca aaagccgcgt cgtacgccgc ctgctggtcc gcggccgagg gtggctcacc
     1261 cgccgccgcc ggggcagccg caaccgtcga cgacgccgcg gcgccggagc cctcgcgcgg
     1321 cggctcgggc acgcccgatt cgagcgcgga aagacgccgg tccaggtcgt tgtacaggtc
     1381 gcgctgctgc tgctttgcac cctccaccgc gttctcctgc acctcactcc tgccctgcag
     1441 ttcgcgggaa tgagcctcga gagcttcgac gcgctgcgaa agttcgacca ggctctggtt
     1501 cgcaatgatg cgctcgactt tcgcgacccg cgcgtcgaga tcggcaaggc ggacctgcac
     1561 cgggtcctct tcggcgggag tcaggctgca cccgccgagc acgatcgacg ccatcagcgt
     1621 gcctgcgagt ggaagtctcg gatgtcgcat gcgggctccg ctcagttcag gtagacgatt
     1681 tcgacgcggc ggttctgtgc ccaggccacc tcgtcgctgc cggcggaggc gggccgctcc
     1741 tcgccatagc tgacggtcga cacctgggtt tcggcgactc cctgcagcag gaacgcgcgt
     1801 cgcacggact gcgagcgccg ctcgccgagg ccgatgttgt actcgcgcgt gccccgctcg
     1861 tcggtgtggc cttcgaggcg caccttcgca cccggattgc cggcgaggta gcgtgcgtgc
     1921 gcattgacga tcgcggtatc ctgcgcgttg agctcggtgc gatcgaactc gaaatagacg
     1981 atgcgcttgc tgagcagctc gccttgcgga cccgcgctcg ccgtgtcgtc gccgagcggt
     2041 gcggtcgtga ccgccccgtc accgacgccc tgacccaccg ccgcctcggt acccgcgccc
     2101 tgcgctgtgt ccgtggccgg cttctccgcg cccttcttcg aggcgcagcc cccgaccgcg
     2161 agagcgcccg cggccagcaa tacccagatc agatttcgca ttgcatcgct acctttgctg
     2221 tcagtcatgg ccgccatggg ccccacacgg gttctcggac ctcgccgcga tccgccttca
     2281 gccgctgtgt cgtctgccca tcgatcgcga tggtcgcgag ggttccctgt ccgcgttcac
     2341 ggcccgcata gatgagcatc gccccattcg gggcgaagct cggcgattcg tcgagcggcc
     2401 ccttcgagag ggttcgcacg actccggtcg caaggtcctg cacggccacc cgataggcac
     2461 cgcccgcgag ggtcaccatc gccagctgtt tgccgtccgg cgacagtcgc ccgcgtgcat
     2521 tgtagctccc cgtaaaggtc acgcgcttcg ccgtcgcgtt ggcgagcacg tcggcgcgat
     2581 agatctgcgg gctgcccgcc cggtccgacg tgaaatagag agtgcgtccg tcagggctga
     2641 acaccgcctc ggtatcgatc gcggagtcgc tggtgatgcg tgtgagcgca ccgctttcga
     2701 gctcgaggac atgaatgtcg agattgccgg tactgcctga caaggtcacc gcgaggcgct
     2761 tgccatcggg cgagaacgcc ggcgagccgt tgatgccggc acgcgcaatc acgcgcctgc
     2821 gctcgccggt cgacacgcgc tgcatccaga tcgccgagct gcgcgattcg aacgacacgt
     2881 aagcgagcca ttcgccgtcc ggcgaccacg ccggcgacat cagcggcagc ggcgactcga
     2941 cgacggtgcg cgccgcctcg ccgtcggcgt ccgccacgat caggctgtag gtctgccttg
     3001 gcggctgacc gctcaccgcc acataggcga tgcgggtcgc gaatgcgccc ggtacaccga
     3061 gcactttttc gtagatcagg tcactcaccc ggtgggcggc ctcgcgctgc cggccggccg
     3121 gggcgacgaa gcgctggccg gcgatgcgct ggccgttgac gagattcacg agttcgacct
     3181 cgatctgcgc ggcgccgccc tcacccggca gtacgcggcc gacgacgatg tagtcgttgc
     3241 gactcgtgcg ccagtccggc accacgatgt cggccgccct cgacggcgtc tcgatcatgt
     3301 cgcggcgggc catcgcgcgg aaccggccac tgccctcgag gtcgcgctgc acgatctccg
     3361 cgacgtcgat cccgccgtcg ccgggcaccg accgggcaaa cggaacgatg gcgatcggga
     3421 tcgggtcggt caccccggag gtgatctcga tgcgcagctg tgcatgggcg gccgtcgccg
     3481 cgagtgcgcc gaccagcatg cacaggatga acaggaaccg ggcgggaagt ctggcgtcag
     3541 tcattgggct tgaataccac gacgaggttg cgttcgaaca gggacggatc cggcggcgcc
     3601 ggcagcggcg aggcgctcat cacggcgatc tcgatcgact gccgcacggc ggcatcgcca
     3661 ttgcaggcga ggatgcgcac ggcgaccacc tcgccacccg gcacctgcgt gacctgcacc
     3721 tcgcactcga tgcccggctg cgcagtgggc ggacggcgcc actcgctttc gatttttgca
     3781 cgaatcaggt cgcgccagtg cgcgccggcg ccgctcgcac gcgccgcacg cagtctttct
     3841 tcggccgcaa tcgaccgcct gagttccgct tcgcgctcgg cacgccggcg cgcctcttcg
     3901 gccttgcgtt ccgcctcggc ctggcgttcc gcctcggcct ggcgttccgc ctcggccttg
     3961 cgttccgcct cggcctggcg ttccgcctcg gccttgcgtt ccgcctcggc ctgg
//
LOCUS       DAOQVY010000163         3933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_25956_length_3933_cov_5.646209, whole genome shotgun sequence.
ACCESSION   DAOQVY010000163 DAOQVY010000000
VERSION     DAOQVY010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25956_length_3933_cov_5.646209"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="PKN91_11690"
     CDS             complement(<1..899)
                     /locus_tag="PKN91_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28770.1"
                     /translation="MALEDGYVEIDGKKYQIVTLDFETYYDKDYTLSGKMNLSEYVRD
                     DRFHAHGVGIKIGNGKTLWYTGKNIPLALREIDWSKCAMLAHNTAFDGFICSQVYDIK
                     PAFYLDTLSMARAAHGHHMRHDLDTLAKAHGLAGKVKRDALTDTKGKQQLTDKEERAL
                     GAYCVDDVEDTYKIFWKLHDYIPDKELQLIDLTLRMFCDPVLRIDIPRVQAELEREVG
                     GKTAALLRSGASVEDLMSNEKFAELLRRAGVNPPMKISPSTGKLTYAFAKSDLDFQTL
                     LQHPNEKVRALADSTLRRVPITAS"
     gene            complement(889..3267)
                     /locus_tag="PKN91_11695"
     CDS             complement(889..3267)
                     /locus_tag="PKN91_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNS28771.1"
                     /translation="MGVQTMPRENRSPKAGSRTPPLFKHQIESVKFMSTRPRVLDASD
                     PGCVSCDTEYLTPTGWKRIDQYTPGDLVAQFHPEDCSIEFVEPISYIKEPCTEMVAIT
                     PVRGTSQLLSPEHRVLWYSNDGSTFGEQSAEDFMYELHKKGPNRLNKKFMTAMRTRAT
                     GTTGLSDAELRVQVAFVADGHLSAKTPGSKGTVRLKKARKIERMHMLLKAAGIEYRYR
                     VQPSTGFTIFRFTPPLHYKHFPNEYHGLNQHELCVIGDEATYWDGSFDPRPSSGRKFS
                     SMVKSDADIVQLAWAALGASTSVHWSKHGGYQVYASWKPLVGPGRKTSVGLTPSPDGF
                     KYCFTVPSTFLYLRRNGRPFATGNTGKTRVQIELFAVRRKRGGGKALVIAPKSLLRSA
                     WQDDFKKFAPEIKTVVCTADKRDQYFAQEADVYITNTDAVRWLAQQPAKFFAQFDTLI
                     IDELSAFKHHTSQRSKAINKIKKHFKYRYGLTGTPNSNSITDIWHQIFILDDGQRLGK
                     SFYQFRNAVCQPRQVGPQPNMLQWEDKPGAELAVGQLLADMVVRHKFEECVDIPANHT
                     YSVPYHMSPKQMKAYQQMEKAAITQLANGSIVSAVNAAGVMTKLLQIASGASYTDGLG
                     GEDYALIDDGRYELVGDLVEQRDHSVVFFNWAHQRDLLIKEFEKRGITYAVIDGKTSD
                     KARKEAVDHFQAGFYRALLAHPQSAAHGLTLTKGTATIWASPTYNLEHFLQGNRRIYR
                     AGQTQKTETIVVLAPGTVEDKVFQKLQEKNVRQGNMLNLLKELFHEEMPNGA"
     gene            complement(3346..>3933)
                     /locus_tag="PKN91_11700"
     CDS             complement(3346..>3933)
                     /locus_tag="PKN91_11700"
                     /inference="COORDINATES: protein motif:HMM:NF014040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNS28772.1"
                     /translation="GKTEHANDRGTRIHNAAELFVQGGIELIPELHSFREEFLRLREL
                     FKQGKVSLEGEWAVDRNWEPTAWHSSNVWARIKLDAFVRMSKTHGVVIDYKTGKKFGN
                     EIKHAEQCQLYQLAAFLRYPELEVIDVELWYTDQNDLTHMRYTREQGLRFYRNFDNRG
                     NAITTATEFPPNPNMFSCRWCPYGPRGTGHCDKGV"
BASE COUNT          704 a         1103 c         1192 g          934 t
ORIGIN      
        1 gaggccgtga tcgggacgcg gcgtaaggtg ctgtcggcca acgcgcggac cttctcattg
       61 gggtgctgga gcagcgtctg gaaatcgaga tccgacttgg cgaaagcata ggtcagcttg
      121 cccgtcgacg ggctgatctt catcggtgga ttgacacccg cgcgacgcag cagctcggcg
      181 aatttctcgt tcgacatgag gtcttcgacg cttgcgccgc tgcgcagaag agcagcagtc
      241 ttgccaccga cttccctctc caactccgcc tgtacgcggg ggatgtcgat gcgcaggact
      301 gggtcgcaga acatgcggag ggtgagatca atcagctgca gttccttgtc cgggatgtag
      361 tcgtgaagct tccagaagat cttgtacgta tcctccacgt catcgacgca gtacgcaccg
      421 agggcacgct cctccttgtc ggtcagctgc tgcttgccct tggtgtcggt cagcgcgtcg
      481 cgcttcacct tgccggcgag accatgcgcc ttggccaggg tatcgaggtc atggcgcatg
      541 tggtggccat gcgccgcgcg agccatggac agtgtgtcga ggtagaacgc cggcttgatg
      601 tcgtacacct gcgagcagat gaacccgtcg aaggccgtgt tgtgggccaa catggcacac
      661 ttggaccagt cgatctcccg cagcgccagc gggatgttct tgccggtgta ccagagggtt
      721 ttaccgttgc cgatcttgat ccctacgccg tgcgcgtgga agcgatcgtc tcgaacgtac
      781 tcggacaagt tcatcttccc cgacagggtg tagtccttgt cgtagtaggt ctcgaagtcg
      841 agcgtgacga tttggtactt cttgccgtcg atctcgacgt atccgtcctc aagcgccatt
      901 gggcatctcc tcatggaaca gttctttcag cagattgagc atgttgccct gtcggacgtt
      961 tttctcctgc agtttctgaa agaccttatc ttccacggtg ccgggtgcca gcaccacgat
     1021 ggtctcggtc ttctgcgtct gtccggcgcg gtagatgcgt cgattgccct gcaggaagtg
     1081 ctcgaggttg tacgtgggcg atgcccagat agtggccgtg cccttcgtaa gagtaaggcc
     1141 atgcgccgcg ctctggggat gagccaacag cgcgcggtag aaaccggctt ggaagtgatc
     1201 gacggcttcc ttgcgggcct tgtcactggt cttgccgtcg atcaccgcgt aggtgatgcc
     1261 gcgcttctcg aactccttga tcaggaggtc gcgttggtgt gcccagttga agaagacgac
     1321 gctgtggtca cgttgctcaa cgagatcgcc caccagctca taccggccat cgtcgataag
     1381 ggcatagtcc tcccctccca acccgtcggt gtaagaggct ccgctcgcga tctgcaacag
     1441 cttggtcatc acccctgcgg cgttgaccgc tgatacgata gagccgttcg ccagttgggt
     1501 gatggcggct ttttccatct gctggtacgc cttcatctgc ttgggcgaca tgtggtacgg
     1561 cacggagtag gtgtgattgg cagggatgtc gacgcattct tcgaacttgt ggcgcacgac
     1621 catatcggcg aggagctggc caacagcgag ttccgctccg ggcttatctt cccactgcag
     1681 catgttcggc tgcggtccga cctgacgcgg ttggcagaca gcgttacgga actggtagaa
     1741 cgacttaccc agccgctggc cgtcgtcgag gatgaaaatc tgatgccaga tgtcggtgat
     1801 cgagttggag ttcggcgtgc cggtgaggcc gtatcggtat ttgaagtgct tcttgatctt
     1861 gttgatcgcc tttgaacgct gactggtatg gtgcttgaac gccgagagct cgtcgatgat
     1921 cagcgtgtcg aactgcgcga agaatttcgc cggctgctgg gccagccatc ttaccgcgtc
     1981 ggtgttggtg atgtacacgt cggcctcttg tgcgaaatac tgatcgcgct tgtcggccgt
     2041 gcacacgaca gttttgatct cgggggcgaa cttcttgaag tcgtcctgcc aagcactgcg
     2101 gagtagcgac ttgggggcga tgaccagcgc cttgcccccg ccacgcttgc gacgtactgc
     2161 gaagagctcg atttgcacgc gagtcttacc cgtattgccc gtcgcaaacg gacgcccatt
     2221 cctgcgtaga tacaagaacg tgctgggcac cgtgaaacag tatttgaagc catctgggga
     2281 aggggtcaat ccaaccgaag tcttgcgccc gggcccaacg agtggcttcc aactcgcgta
     2341 tacctggtac ccaccgtgtt ttgaccaatg gaccgatgta cttgcgccaa gggctgccca
     2401 tgccaactgc acaatatcgg catcgctttt caccatactc gaaaatttgc gtcccgagct
     2461 gggacgtggg tcgaatgacc catcccagta ggttgcttca tccccgatta cgcacaactc
     2521 gtgttgattt agtccgtgat actcgtttgg gaagtgtttg tagtgcagcg gtggtgtgaa
     2581 tctgaatata gtgaatccgg tgcttggttg gacgcgatag cggtattcga tcccagccgc
     2641 tttcagcagc atgtgcatgc gctcgatctt gcgcgctttt ttgagacgca cagtaccttt
     2701 gctgccgggg gttttcgcgg acaagtgccc gtctgctaca aacgccacct gtacgcgcag
     2761 ctcggcgtct gacaacccgg tggtgcctgt tgccctagtg cgcatggctg tcatgaactt
     2821 cttatttaga cggttcgggc cttttttatg cagttcatac ataaaatcct cagccgattg
     2881 ctctccgaaa gtcgatccgt cgttggaata ccatagtacc cggtgctcgg gggagagcag
     2941 ctgagatgtt ccacggacgg gggtaattgc taccatctcc gtacacggtt ctttgatgta
     3001 ggatatcggt tcgacgaatt caatcgaaca atcctcggga tgaaattgcg cgaccaaatc
     3061 cccgggggtg tattggtcga tgcgcttcca ccccgttggg gtgagatatt cagtgtcaca
     3121 agatacacag ccagggtccg atgcgtcgag cacgcgcgga cgcgtgctca tgaacttcac
     3181 gctttcgatc tggtgcttga agagaggcgg cgtgcggctg cccgctttcg ggcttcgatt
     3241 ctctcgaggc atcgtttgta ctcccatgtg tagatcttgt cttcacccag cagtcgcatg
     3301 cgagcggctt tctcgatgtc cagttctgcc aaggtcttgt ccatatcaga cgcccttgtc
     3361 gcagtgcccg gtgccgcgcg gaccgtacgg gcaccaccgg caggagaaca tgttcgggtt
     3421 gggagggaac tcagtcgccg tggtgatcgc gttgccacgg ttgtcaaaat tgcggtagaa
     3481 gcgcagcccc tgctcacggg tgtagcgcat gtgggtgagg tcattctggt cggtgtacca
     3541 gagctcgacg tcgatcacct ccagctcggg gtagcgcagg aacgcagcga gctggtacag
     3601 ctggcactgc tcggcgtgct tgatttcgtt accgaatttc ttgccggtct tgtagtcgat
     3661 caccacgcca tgggtcttcg acattcggac gaaggcgtcc agtttgatgc gggcccacac
     3721 gttactggag tgccatgcgg tcggttccca gttgcggtcg acagcccact cgccttcgag
     3781 gctgactttg ccctgcttga acagctcgcg gaggcgcagg aactcttcgc ggaagctgtg
     3841 caactcgggg atcagttcga tcccgccctg tacgaacagc tcggctgcgt tgtggatgcg
     3901 cgtgccgcga tcgttggcgt gctcggtctt gcc
//
LOCUS       DAOQVY010000164         3866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_26560_length_3866_cov_3.945421, whole genome shotgun sequence.
ACCESSION   DAOQVY010000164 DAOQVY010000000
VERSION     DAOQVY010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26560_length_3866_cov_3.945421"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="PKN91_11705"
     CDS             <1..1059
                     /locus_tag="PKN91_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HNS28773.1"
                     /translation="LETDMHATLRVVRELLVGVEGIAARPIGHRIVLEGLATGARAQD
                     RAAAIATLYPGVVLNFVGKVAWESMIHFDVRIVEVRVGALRDVGVRWRDDINGPNSGL
                     IADLVTNDLFRAVPPAPELDALQGAALPERVWPPKVHLGWITTIDSRIRLLEQQGDAV
                     IVAEPMLSCRSGGSARFVSGGELPIPVFDPQGGADVEYKEYGVILDVKPVAEPDGSIY
                     ARIDTEVSQVDEAQRVLGVPGFIKRQSATEVNLREGETLVIAGLVNRSFSRERRQVPG
                     LGNLPVVGGAFRSRTRRSLDSELAIFITPRIVSGEAARGAPAERPHATAERLQDRIDA
                     LAPPSPPRPAPQPGRPQP"
     gene            1056..2369
                     /locus_tag="PKN91_11710"
     CDS             1056..2369
                     /locus_tag="PKN91_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNS28774.1"
                     /translation="MNPAAAVPAVPLPELARTLHRELLAALDLRRRDIVQMPEAELRA
                     LASERLAGIIGRMSLPRDLDTRALVRFVVDEAIGLGPLESLLADDSVSEIMVNGSGRV
                     FVERGGQLQSTTTRFTSEQALRAAIDRVVSRVGRRVDEASPLVDARLADGSRVNVVLP
                     PLSLCGAALTIRKFSRRRLTMDDLVDLGAISRPMVQFLAECVRHRRNIIVSGGTGSGK
                     TTLLNVLSALIPEGERIITIEDAAELRLAHDNLVALEARTANLEGRGEITIRDLVRNA
                     LRMRPDRIVVGECRGGEALDMLQAMNTGHDGSLTTAHANGPRDLLSRLEVMVLMAGVE
                     LPLQAVREQIAAAVDLIVHVARFPCGARRVTHIVEVTGIDAGVIATQELFHHLRSGSG
                     GAGRFEAAGQVPAFYDTLREGGAVLDYSIFRRDTTVTGTGGGRQP"
     gene            2375..3211
                     /locus_tag="PKN91_11715"
     CDS             2375..3211
                     /locus_tag="PKN91_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNS28775.1"
                     /translation="MALALCAIAAAGAVIGTHQLTRSAASLLRDQGIERPAGALADLF
                     IFIEPDRLLRLSALAGAAGALIVAAVFRSVLSALLAAAIALAAPRCLHWWWRRRYLQR
                     IGAQLPDMITMLAGGVRAGAALAQGLDQLAQRVPAPLGHELALVMRRHRLGVRLEDAL
                     QEMAARMPLEPVRLLVTSVSLALQVGGGLGGTLDRLAGSLRRKQAVEAKLRSLTAQGR
                     LQAIIVTALPFALMLAMFVLDEASMRPLFATASGWMVLGCILVLDLTGWLLIRRIVAI
                     DA"
     gene            3208..>3866
                     /locus_tag="PKN91_11720"
     CDS             3208..>3866
                     /locus_tag="PKN91_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28776.1"
                     /translation="MSTSWDPAMLASALAAGVAAACAVLACVRGTLGARVRPPRDCTG
                     LPVWLRAALPMAAGPARLIGPLLPPALVQNLQARLRASDLDDTVSVAEWCALHLVMSA
                     AVVPAAWLGGGRIGVLVLAAIAAASWPTLWLRDRRARLERGIDRDLPRLLELVTLAVE
                     AGCSLGVALRIGVQSGGSGALPRGLAEALRAIHAGRARTDALAELAARYRHAGSARPV
                     RP"
BASE COUNT          522 a         1354 c         1357 g          633 t
ORIGIN      
        1 ctcgaaaccg acatgcacgc cacactgcgc gtcgtgcgcg aactgctggt cggcgtcgag
       61 ggcatcgcgg cacgcccgat cggccatcgc atcgtgctcg agggcctcgc caccggagcc
      121 cgtgcgcagg acagggccgc ggcgatcgcc acgctgtacc ccggtgtggt gctcaacttc
      181 gtcggcaagg tggcctggga gagcatgatc cacttcgacg tacgcatcgt cgaagtccgc
      241 gtcggagcgc tgcgtgatgt cggcgtgcgc tggcgcgacg acattaacgg acccaattcg
      301 gggctgatcg ccgacctcgt caccaacgac ctgttccgcg cagttccccc cgcaccggaa
      361 ctcgacgcgt tgcagggtgc ggcgctgccg gagcgcgtgt ggccgcccaa ggttcatctc
      421 ggctggatta ccaccatcga ttcgcgcatc cgcctgctcg agcagcaggg ggatgccgtc
      481 atcgtcgccg aaccgatgct gagttgccgc agcggtggca gtgcgcgttt cgtgtcgggt
      541 ggcgagctgc ccattcccgt gttcgatccg cagggcggcg ccgatgtcga atacaaggag
      601 tatggcgtca tcctcgacgt gaagcccgtg gccgagcccg atggcagcat ctatgcgcgc
      661 atcgacaccg aagtgagcca ggtggacgag gcgcagcgcg tgctcggcgt gccgggcttc
      721 atcaagcgtc agtccgccac cgaagtgaac ctgcgcgaag gggagaccct cgtcatcgcc
      781 ggcctcgtca atcgcagctt ctcgcgcgag cgccggcaag tgccgggcct cggcaacctg
      841 ccggtcgtcg gcggcgcgtt tcgttcgcgt acccggcgca gccttgactc cgagctcgcg
      901 atctttatta ccccgcggat cgtatccgga gaggcggcac gcggcgcgcc ggcagagcgt
      961 ccccacgcga ccgccgagcg gctgcaggat cgcatcgatg cactggcgcc accgtcgccg
     1021 ccgaggcccg caccacagcc cgggaggccg cagccgtgaa tcctgctgcc gccgtccctg
     1081 cggtaccgct gcccgaactc gcccgcacgc tgcatcgcga actgctggcg gcactcgatc
     1141 tgcgccggcg cgacatcgtg cagatgcccg aagcggagct gcgcgcgctc gcgagcgaac
     1201 gccttgccgg gatcatcgga cggatgagcc tgccacgaga cctcgacacg cgcgcactcg
     1261 tgcgcttcgt cgttgacgag gccatcggcc tcggaccgct cgagagcctg ctcgcagacg
     1321 acagcgtgtc ggagatcatg gtcaacggct ccggccgcgt gttcgtcgag cgcggcgggc
     1381 agctgcaatc gacgaccacg cgcttcacga gcgaacaggc gctgcgggcc gcgatcgatc
     1441 gcgtggtgtc gcgcgtcggc aggcgggtcg acgaggcttc accactcgtc gacgcgcgac
     1501 tcgccgacgg ctcgcgcgtc aacgtcgtgc tcccgccgct gtcgctgtgc ggcgccgcgc
     1561 tgacgatacg caagttctcg cgccggaggc tgaccatgga cgacctggtc gacctcggcg
     1621 cgatcagccg gcccatggtg cagttcctcg ccgaatgcgt gcgccaccgg cgcaacatca
     1681 tcgtctcggg cggcaccgga tcgggcaaga ccacgctgct gaacgtgctc tcggcgctga
     1741 tcccggaggg cgagcgcatc atcacgatcg aggatgccgc cgagctgcgt ctcgcccacg
     1801 acaatctggt ggcgctcgag gcgcgtaccg ccaatctcga gggtcgcggc gagatcacga
     1861 tccgcgacct cgtgcgcaat gcgctgcgca tgcgtcccga tcgcatcgtc gtcggcgaat
     1921 gccgcggcgg cgaggcgctc gacatgctgc aggcgatgaa caccgggcac gatggcagcc
     1981 tgacgacggc ccatgccaat ggtccccgcg acctgctgtc gcgcctcgag gtgatggtgc
     2041 tgatggcggg agtcgaactg ccgttgcagg cggtgcgcga acagatcgcc gccgcggtcg
     2101 acctgatcgt gcacgtggcg cgcttcccct gcggcgcacg ccgtgtgacc cacatcgtcg
     2161 aagtcacggg catcgatgcc ggtgtcatcg cgacgcagga actgttccat cacctgcgct
     2221 ccggctccgg gggcgcggga cggttcgagg cggccggcca ggtgccggcc ttctatgaca
     2281 ccttgcgcga gggcggtgcg gtgctcgact actccatctt tcgccgcgat accacagtca
     2341 ccggcactgg tggcgggcgc caaccgtgat ctacatggcg cttgccctct gtgccatcgc
     2401 tgcggccggc gccgtgatcg gcacccatca gctcacgcgt tcggcggcct cgctgctgcg
     2461 cgaccagggc atcgagcggc cggccggcgc actcgccgac ctgttcatat tcatcgagcc
     2521 tgaccggctg ctgcgtttga gtgcactggc cggtgctgcc ggcgcactca tcgtggcggc
     2581 ggtgttccga tcggtgctgt cggcactgct cgcagcggcg atcgcgcttg ccgcaccgcg
     2641 ctgcctgcac tggtggtggc gccggcgtta cctgcagcgc atcggcgcgc agctgcccga
     2701 catgatcacg atgctggcgg gtggggtgcg ggcgggcgca gcgctggcgc agggactcga
     2761 tcagctggcg caacgcgttc cggcacctct cggccacgag ctcgcgctgg tcatgcgccg
     2821 ccaccggctc ggcgtgaggc tcgaggatgc attgcaggaa atggccgcgc gcatgccgct
     2881 cgagccggtg cgcctgctgg tgacctcggt ttcgctcgca ctgcaggtcg gaggcgggct
     2941 gggcggcacg ctcgatcggc tggcgggcag cctgcgccgc aagcaggccg tcgaggccaa
     3001 actgcgctcg ctgactgcgc agggccggct gcaggccatc atcgtcaccg cattgccgtt
     3061 cgcgctgatg ctcgccatgt tcgtactcga tgaggcgtcg atgcggccgc tgttcgcgac
     3121 cgcgtcgggc tggatggtgc tcggctgcat cctggtcctg gatctgaccg gatggctgct
     3181 catccgccgc attgtggcga tcgacgcatg agcacgtcgt gggatccggc catgctcgcc
     3241 agcgcgctcg ccgcgggtgt cgcggcggcc tgcgcagtac tggcctgcgt acgcggaaca
     3301 ttgggcgcgc gagtgcgccc gccgcgcgac tgcacaggcc tgcccgtgtg gctgcgtgcg
     3361 gccctgccga tggcggcagg tccggcacgg ctgatcgggc cgctgttgcc cccggccctc
     3421 gttcagaacc tgcaggctcg tctgcgggcg tctgacctcg acgatacggt gtccgtcgcg
     3481 gaatggtgcg cgctgcacct ggtgatgtcc gcggcggtgg tgcccgcggc atggctcggc
     3541 ggtggtcgga tcggcgtgct ggtcctcgct gcgattgccg cggcgagctg gcccacgctg
     3601 tggctgcgcg atcgccgcgc gcgtctcgag cgcggcatcg atcgcgacct tcctcgcctg
     3661 ctcgaactcg tcacgctcgc cgtcgaagcc ggctgctccc tgggcgtggc gctacgcatc
     3721 ggcgtgcagt ccggcgggtc gggtgcgctc ccacggggac tcgccgaggc gctgcgggcg
     3781 atccacgccg gccgtgcgcg caccgatgcc ctggccgaac tggccgcgcg ctaccggcat
     3841 gccggtagcg cgcggccagt tcggcc
//
LOCUS       DAOQVY010000165         3832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_26898_length_3832_cov_5.076781, whole genome shotgun sequence.
ACCESSION   DAOQVY010000165 DAOQVY010000000
VERSION     DAOQVY010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26898_length_3832_cov_5.076781"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PKN91_11725"
     CDS             <1..191
                     /locus_tag="PKN91_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28777.1"
                     /translation="LQYQVGIIAGAGFLPYPPGAESECAKLMYVAMTRATHELLITSS
                     RNSSFSARLRGICEPLAA"
     gene            405..2825
                     /locus_tag="PKN91_11730"
     CDS             405..2825
                     /locus_tag="PKN91_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNS28778.1"
                     /translation="MRMHRTWMRQLPGFAALTLFLPMAAHTQEPDTQSGALEEVVVTA
                     QKRSESAQNVPFSVAAVTEEKMERSGSNNIVELARNIAGLTITDLGPGQSQVAIRGIS
                     AGQVVRDQAGVKESVGIYLDESPISVALFTPDLDLFDLERFEVLRGPQGTLFGAGSTS
                     GTVRYITRAPQLERLEGSVQLTAHEGTDSEFGGAVKAAVNLPVGDRVALRVTGYYDEL
                     AGFIDSHYPGRPVREDVNGGERSGGRIAMLFEPNDQLSITPRVVYQKLETDGFPRIEA
                     YSILGNPFTTSEPAVSPGERGQVTQIREGIDDEFMLADLTLHYDFGGVTLTSVTSYTD
                     RDVVVTRDAGQLTGSVTYDLTFGHPTGYFPEEVRLDSPLIDATQLEAISQEIRLASGD
                     EGPFVWLVGGFYQQVDRDYGQTLPTPGYDAILQRRFGLTSVDFGAPPDMPYFSDLTYD
                     FEQFGLFGEGTWRFNDQWSLTGGVRYYDFEEDRLLTFAGVFADMGYTDEPGSTSSDGF
                     SPRAILSYRPNDDVMITAQAARGFRLGGINDPLNVTLCQGNDIDIYGGHPTWDDEKVN
                     NFELGIKSRLAEGRVLFNAAAFYSDIDGLQVIADAGSCSSRIILNAEAESIGAEVELF
                     ARPSDNWDFGLSATYVQAEITKSQVDPNGNPIAGIRKGNRLPTSPEFQAAASATYSWS
                     WTTTLESYLTFTVQHVGSSFTQLADQEPNFGLIATGGPPGAARFIPFGDPTIDSFAFA
                     AELPSYEIGNLRFGLHSGNWELAAFINNLWDERAFLSIDRERGRSARVGYLTNVPRTY
                     GLTLNYAF"
     gene            complement(2835..3458)
                     /locus_tag="PKN91_11735"
     CDS             complement(2835..3458)
                     /locus_tag="PKN91_11735"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNS28779.1"
                     /translation="MTRNLRLAIAASLALLAAGCGGTFTSKAPISRAYVLYPASAAGE
                     PARATPVFDGALRIARPAAGPGFETDRILLLRADRQLDFYANGHWAAPIPDMVGALAA
                     ETLRPRSLFASVYGDAVALRADYFLQIVVRAFVADYSRGGEAPVAQVLLDCTLVQRLD
                     RAVLANFTVRGEVPAAANRMTEVVAAQEQALQQALESAAASIAAAAR"
     gene            complement(3455..>3832)
                     /locus_tag="PKN91_11740"
     CDS             complement(3455..>3832)
                     /locus_tag="PKN91_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28780.1"
                     /translation="HFPGMMRDAESLVAELRSSSRDFSATMVVMRNLTENSAPDVAEA
                     LAALRVASTNLASASAGVDRFITENERELRSFARDALPEVERLLQDGRSAAAEFRDLS
                     RQLREDPGQLLYESNYFGVEIPK"
BASE COUNT          629 a         1319 c         1288 g          596 t
ORIGIN      
        1 ggctgcagta ccaggtcggc atcatcgccg gcgcgggctt cctgccctat ccgcccggcg
       61 ccgagtccga atgcgcgaag ctgatgtatg tcgcgatgac ccgcgcgaca cacgagctgc
      121 tgatcaccag cagcaggaac agcagtttca gcgcgcgctt gcggggaatc tgcgagccgc
      181 tcgcggcctg acggcggcgg tgaaacgcga tgtccgggct cgtcctgcta ccactaggag
      241 gcagtggggc cagcaatccg ttgcgtaaaa agaacatcct gatgccggcc aggcgcggcg
      301 cttttccgcg gcccctgctt ccaaatcggc gcgtgcaggt gcactatttc gcacaagtgc
      361 aacagcgggc cggccccgcc acccgcgaca ccaaggagtg cgacatgcgg atgcacagga
      421 catggatgcg gcagcttccc ggcttcgccg cgctgactct gtttcttcca atggccgccc
      481 acacgcagga gcccgacacg cagagcggcg cgctcgagga agtcgtggtg accgcgcaga
      541 agcgcagcga atcggcccag aacgtgccgt tctcggtggc cgcggtcacc gaagagaaga
      601 tggagcgctc gggttcgaac aatatcgtcg agctggcgcg caatatcgcc ggcctcacga
      661 tcaccgatct cgggcccggc cagagccagg tcgcgatccg cggcatcagc gccggccagg
      721 tggtgcgcga ccaggcgggc gtgaaggagt cggtcggcat ctatctcgac gagtcgccga
      781 tttcggtcgc gctgttcacg cccgatctcg acctgttcga tctcgagcgc ttcgaggtgc
      841 tgcgcggtcc gcagggcacg ctgttcggcg cgggttccac ctccggcacc gtgcgctata
      901 tcacccgggc gccgcagctt gagcgtctcg aaggcagcgt gcagttgacc gcccacgaag
      961 gcaccgacag cgagttcggc ggcgcggtca aggcggccgt caatctgccg gtcggcgatc
     1021 gggtcgcact gcgcgtcacc ggctattacg acgagctcgc cggcttcatc gactcgcatt
     1081 atccgggccg gccggtgcgc gaagacgtga acggcggcga gcgcagcggc gggcgcatcg
     1141 ccatgctctt cgagccgaac gaccagctct cgatcacgcc gcgcgtcgtt taccagaagc
     1201 tcgagaccga cggtttcccg cgcatcgagg cctacagcat cctcggcaat ccgttcacga
     1261 ccagcgaacc ggcggtctcg cccggcgagc gcggccaggt cacgcagatc cgcgagggta
     1321 tagacgatga gttcatgctg gccgacctga cgctgcatta cgacttcggc ggcgtcacac
     1381 tcacttcggt cacctcgtat accgatcgcg atgtggtcgt cacgcgcgat gcgggccaac
     1441 tcaccggcag cgtcacctac gacctgacct tcgggcaccc gaccggctat ttccccgagg
     1501 aggtgcggct cgattcgcca ctcatcgacg cgacgcagct cgaggcgatc agccaggaga
     1561 tccgtctcgc ctcgggcgac gagggcccgt tcgtgtggct ggtcggcggc ttctaccagc
     1621 aggtcgatcg cgactacggc cagaccctgc cgaccccggg ctacgacgcg atcctgcagc
     1681 ggcgcttcgg cctgacgagc gtcgatttcg gcgcgccgcc cgatatgccg tatttctcgg
     1741 acctgaccta cgatttcgag cagttcgggc tgttcggcga aggcacctgg cgtttcaacg
     1801 accagtggag tctgaccggc ggcgtgcgct actacgactt cgaggaggac cgcctgctca
     1861 ccttcgccgg cgtgttcgcc gacatgggct acaccgacga gcccggctcc acgagctcgg
     1921 acggcttctc gccccgcgcc atcctctcgt accgcccgaa cgacgatgtg atgatcacgg
     1981 cgcaggccgc gcgcggcttt cgcctcggcg gcatcaatga tccgctgaac gtcacgctct
     2041 gccagggcaa cgacatcgac atctacggtg gccacccgac ctgggacgac gagaaggtca
     2101 acaacttcga gctcggcatc aagagccgtc tcgccgaggg ccgggtgctg ttcaatgccg
     2161 cggcgttcta ctccgacatc gacggcctgc aggtgatcgc cgacgcgggc tcgtgctcgt
     2221 cgcgcatcat cctgaacgcg gaggccgagt cgatcggcgc cgaggtggag ctgttcgcgc
     2281 gcccgagcga caactgggac ttcggcctgt ccgcgaccta tgtgcaggcg gagatcacca
     2341 agtcgcaggt cgacccgaac ggcaacccga tcgccggtat ccgcaagggc aaccgcctgc
     2401 cgacttcgcc ggagttccag gcggcggcga gcgccaccta ctcgtggtcg tggacgacga
     2461 cgctcgagag ctacctgacg ttcaccgtgc agcacgtcgg ttcgtccttc acccagctcg
     2521 cggaccagga gccgaacttc gggctcatcg ccacgggcgg gccgccgggc gcggcgcggt
     2581 tcattccgtt cggcgatccg acgatcgact cgttcgcctt tgccgcggag ctgccgtcct
     2641 acgagatcgg caacctgcgc ttcggcctgc acagcggaaa ctgggagctc gccgccttca
     2701 tcaacaatct gtgggatgag cgcgcgttcc tgtcgatcga ccgcgagcgc gggcgcagcg
     2761 cgcgggtcgg ttacctgacc aacgtgccgc gcacctacgg gctgacgctg aactacgcct
     2821 tctgaggcac gcgctcaccg agccgccgcc gcgatcgacg cggcggcgga ttcgagcgcc
     2881 tgctgcagcg cctgctcctg cgcggcaacg acttccgtca tgcgattggc tgcggccggc
     2941 acttcgccgc gcacggtgaa gttcgcgagc acggcgcgat cgaggcgctg cacgagcgtg
     3001 cagtcgagca gcacctgcgc gaccggcgcc tcgccgcccc gtgaatagtc cgccacgaac
     3061 gcgcgcacga cgatctgcag gaaatagtcc gcacgcagtg ccacggcgtc gccgtagacc
     3121 gacgcgaaca gcgaccgggg ccgcagggtc tcggccgcca gcgcgccgac catgtcggga
     3181 atgggcgcgg cccaatgacc gttcgcgtag aagtcgagct gccggtcggc gcgcagcagc
     3241 aggatgcggt cggtctcgaa tccggggccg gccgccgggc gggcaatgcg cagcgcgccg
     3301 tcgaacaccg gcgtggcgcg ggccggctcg ccggcggcgg acgccggata cagcacgtac
     3361 gcgcgggaaa tcggcgcctt ggaggtgaag gtgccgccgc agcctgccgc gagcagcgcc
     3421 agggaggcgg cgatggcgag gcggaggttg cgcgtcactt gggtatctcc acgccgaaat
     3481 agttcgactc gtacagcagc tggcccggat cctcgcgcag ctggcgcgac aggtcgcgga
     3541 actccgccgc cgccgagcgc ccgtcctgca gcagccgctc gacctcgggc agggcgtcgc
     3601 gcgcgaagct gcgcagctcg cgttcgtttt cggtgatgaa gcgatcgacc ccggccgacg
     3661 cgctcgcgag attggtcgac gcgacgcgca gcgctgccag cgcttcggcg acgtcggggg
     3721 cggaattctc ggtgaggttg cgcatcacga ccatcgtcgc gctgaagtcg cgcgaggagc
     3781 tgcgcagttc ggcgaccagt gattcggcgt cgcgcatcat gcccggaaag tg
//
LOCUS       DAOQVY010000166         3808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_27129_length_3808_cov_4.399147, whole genome shotgun sequence.
ACCESSION   DAOQVY010000166 DAOQVY010000000
VERSION     DAOQVY010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27129_length_3808_cov_4.399147"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PKN91_11745"
     CDS             complement(<1..796)
                     /locus_tag="PKN91_11745"
                     /inference="COORDINATES: protein motif:HMM:NF015286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HNS28781.1"
                     /translation="MSALSVFDIFKVGIGPSSSHTMGPMRAAREFVVDLGSDGLLGRI
                     ASVYVRLYGSLALTGAGHGTDRAVMVGLEGADPATVEPDQMQRDVERISSSHRLRLLG
                     EQEIAFDVPMQLLFLTHESLPAHPNGMRFTALDRAGQVLREEEIYSVGGGFIVRGGRA
                     PDAGSGRAPPGRSAAGAAAPAVKYPFASSAELLAHCDASGLEIFELLLANETAWRTPA
                     QVREELLGIWQVMQACIERGFRQQGVLPGVLKVRRRAPRLHRVLTES"
     gene            complement(793..>3808)
                     /gene="putA"
                     /locus_tag="PKN91_11750"
     CDS             complement(793..>3808)
                     /gene="putA"
                     /locus_tag="PKN91_11750"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HNS28782.1"
                     /translation="VERVRAGSTSGSPLDAFLREYSLASREGVILMCLAEALLRIPDA
                     ETADRLIADKIAAGDWSDHLGDSESLFVNASTWGLMLTGRIVQVEADTIDSTRNWFGR
                     LASRIGEPVARAALRQSMKILGHQFVMGRSIEEALERAGGKRERAYRYSFDMLGEAAL
                     TAADAARYLEKYRLAIAALAAAPAAARRQETAGIEAAHSISVKLSALHPRYEFAQRER
                     VLAELAPSLLELTRLAREGGIGITVDAEEADRLELSLELVDAVLADPVTRGYDGFGLA
                     VQAYQKRAPQVIDWLAERAAALNRKLCVRLVKGAYWDSEVKRAQERGLASYPVYTRKA
                     NTDVAYLACARRLAAAQGVIYPQLATHNAHTVAYVAEVFAAAGAPFEFQRLHGMGEEL
                     YEHVVGGEWGDHPCRVYAPVGAHADLLPYLVRRLLENGANTSFVNRLVDASLPAESVV
                     ADPVADIDAATQLAHPRIPLPADLYGTERRNSLGANLADGAEVAALHAACARSRVERW
                     HAAPLLADTAQGSAGATREIVNPADDGDIVGTVTEASAGQVDRAVAVALAAQQRWDAQ
                     GGAARAALLDRAADLMAADRPGLIARCVREAGKSLPDSVAEVREAEDFLRYYAARARA
                     EFEAEVPLPGPTGERNVLRLRGRGVFGCISPWNFPVAIYTGQVAAALAAGNAVLAKPA
                     EQTPLCAARVTELLHEAGVPREVLQFVPGEGTIVGAALSAHDDIAGICFTGSTATARA
                     IERSMAARKGAIGTLIAETGGLNAMIVDSSALAEHVVLDAVASGFNSAGQRCSALRLL
                     IVQEEIAPRVLEMLAGFMDELVVGDPALLATDVGPVIDREALEMLESHAAKVSAGARW
                     SHRVALPATLPPGRFFAPLAVEIERIEDLEREVFGPIVHVVRYRAEDIDSVVDAVNAR
                     GYGLTLGVQTRIDSLAQRIARRARVGNVYVNRNMIGAVVGVQPFGGCGLSGTGPKAGG
                     PHYLHRFATEQTISVNTAAVGGNATLLSLTEV"
BASE COUNT          593 a         1368 c         1299 g          548 t
ORIGIN      
        1 tgctctcggt gagcacgcga tgcaggcgcg gcgcgcggcg gcgcaccttg agtacgcccg
       61 gcagcacacc ctgctggcga aacccgcgtt cgatgcaggc ctgcatcacc tgccagatgc
      121 cgagcagttc ctcacgtacc tgcgcggggg ttcgccacgc cgtctcgttg gcgagcaaca
      181 gctcgaagat ctcgaggccg gaggcatcgc agtgcgcgag cagttccgcg ctcgatgcga
      241 atggatactt gaccgcaggc gccgccgcac cggccgccga cctgcccggc ggcgcccgcc
      301 cggagccggc atcgggcgca cggccaccgc gcacgatgaa gccgccgccg accgagtaga
      361 tctcctcctc gcgaagcacc tggcccgccc tgtcgagtgc ggtgaagcgc atgccgttcg
      421 gatgtgccgg cagcgactcg tgcgtcagga acagcagctg catcggcacg tcgaacgcga
      481 tctcctgctc gccgagcagg cgcaggcgat ggctggacga gatgcgctcg acgtcccgct
      541 gcatctggtc aggctcgacg gtggccgggt cggcgccttc gagtcccacc atcaccgcgc
      601 gatcggttcc atggcccgcg ccggtcagcg cgagcgatcc atacaggcgc acgtacacgc
      661 tcgcaatgcg ccccagcagg ccgtcactcc cgaggtcgac gacgaactcg cgcgccgcac
      721 gcatcggacc catggtgtgc gaactcgacg ggccgatacc gaccttgaat atgtcgaaga
      781 cgctgagcgc gctcatactt cggtgaggga cagcaaggtg gcattgccgc ccaccgcggc
      841 ggtgttgacg cttatggtct gctcggtggc aaagcgatgc aggtaatgcg gtccaccggc
      901 cttgggcccg gttcccgaca atccgcagcc gccaaacggc tgcacgccga ccacggcgcc
      961 gatcatgttg cggttcacat agacattgcc gacccgcgcg cggcgcgcga tccgctgcgc
     1021 gagcgaatcg atgcgcgtct gcacgccgag cgtgaggccg tagccgcgcg cgttcacggc
     1081 atccaccacg gagtcgatgt cttcggcccg gtagcgcacc acgtgcacga tcgggccgaa
     1141 gacctcgcgc tcgaggtcct cgatgcgttc gatctcgacc gcaagcggcg cgaagaaccg
     1201 cccgggcggc agcgtggccg gcagcgccac acgatgcgac cagcgcgccc cggcgctgac
     1261 tttcgcggca tgggactcga gcatttcgag cgcctcacgg tcgatcaccg ggccgacgtc
     1321 cgtggcgagc agcgcgggat cgcccaccac gagttcgtcc atgaagcccg cgagcatctc
     1381 gagcacgcgc ggcgcgatct cctcctgcac gatcagcagg cgcagtgccg agcagcgctg
     1441 gcccgcgctg ttgaagccgg acgccaccgc gtcgagcacg acatgttccg cgagggccga
     1501 gctgtcgacg atcatcgcgt tgaggccacc cgtttccgcg atcagtgtgc cgatcgcgcc
     1561 cttgcgcgcc gccatcgagc gctcgatggc ccgcgcggtc gcggtcgaac ccgtgaaaca
     1621 gatacccgcg atgtcatcgt gcgccgagag cgcagcgccg acgatcgtgc cctcgcccgg
     1681 cacgaactgc agtacctcgc gcggcacgcc cgcctcgtgc aacagctcgg tgacgcgcgc
     1741 ggcgcacagc ggcgtctgct cggcgggctt ggcgagcacc gcattgcccg ccgcgagcgc
     1801 cgccgccacc tgtccggtgt agatcgcaac ggggaagttc cacgggctga tgcagccaaa
     1861 gacgccacga ccgcgcaggc gcagcacatt gcgctccccg gtcgggcccg gcagcggcac
     1921 ctcggcctcg aactccgcgc gcgcgcgggc ggcgtaatag cgcaggaaat cctctgcttc
     1981 gcgcacttca gcgacactgt ccggcagtga cttgccggct tcacgaacac aacgggcgat
     2041 gaggcccggg cgatcggccg ccatcaggtc ggcagcgcga tcgagcagcg cggcgcgcgc
     2101 ggcgccgccc tgggcatccc agcgctgctg cgcggcgagc gccacggcta ccgcgcgatc
     2161 gacctgcccg gcgctcgcct cggtcaccgt gccgacgatg tcgccatcgt cggctgggtt
     2221 gacgatctcg cgggtcgcac cggcgctgcc ctgggcagta tcggcgagca gcggcgcagc
     2281 gtgccagcgc tcgacgcggc tgcgtgcaca ggccgcatgc agggcggcga cctcggcgcc
     2341 atcggcgagg ttggccccga gggagttgcg acgctccgtg ccgtagagat ccgcaggcag
     2401 cggtatgcgc ggatgggcga gctgggtcgc ggcgtcgatg tcggcaaccg gatcggcgac
     2461 gaccgactcg gccggcaggc tcgcatcgac gagacggttc acgaacgagg tgttggcgcc
     2521 gttctccagc aggcggcgca cgagatacgg cagaaggtcg gcgtgtgcgc ccaccggcgc
     2581 atacacgcgg cacggatggt cgccccactc accaccgacc acgtgttcat agagttcctc
     2641 gcccatgcca tgcaggcgct ggaactcgaa cggagcgccg gctgccgcga acacctcggc
     2701 gacataggcc acggtgtgcg cgttgtgtgt cgcgagctgc gggtagatca ccccctgcgc
     2761 cgccgcgagg cgccgcgcgc aggcgaggta ggccacgtcg gtgttcgcct tgcgcgtata
     2821 caccggatag gaggccaggc cacgctcctg ggcgcgcttc acctcgctgt cccagtaggc
     2881 acccttgacg agtcgcacac acagcttgcg attcagtgcc gcggcacgct cggcgagcca
     2941 gtcgatcacc tgcggcgcgc gcttctggta agcctgcacc gcgagcccga agccgtcgta
     3001 accgcgcgtc accggatcgg cgagtaccgc gtcgacaagc tcgagcgaca gttcgagccg
     3061 gtcggcttcc tcggcatcga cggtgatgcc gatgccgcct tcgcgcgcca ggcgcgtcag
     3121 ctccagcagg ctcggcgcga gttcggcgag cacgcgctcg cgctgtgcga attcgtaacg
     3181 cggatgcagt gcggacagct tcaccgagat gctgtgcgcg gcctcgatgc cggccgtctc
     3241 ctgcctgcgc gcggcggcag gagccgccgc cagggccgcg atcgccaggc ggtacttctc
     3301 gaggtagcgc gctgcgtcgg ccgccgtgag cgccgcctcg cccagcatgt cgaacgaata
     3361 gcggtaggcg cgttcgcgct tgccgccggc acgttcgagc gcctcctcga tcgaccggcc
     3421 catgacgaac tggtggccga ggatcttcat cgactgccgg agcgccgcgc gcgccaccgg
     3481 ctcgccgatg cggctcgcca ggcgtccgaa ccagttgcgc gtcgagtcga tggtgtcggc
     3541 ctcgacctgc acgatccgcc cggtcagcat caggccccag gtcgaggcgt tcacgaacag
     3601 cgactcgctg tcgcccaagt gatcggacca gtcaccggcc gcgatcttgt cggcgatcag
     3661 gcggtcggcg gtttccgcgt cggggatccg cagcagcgcc tctgcgaggc acatcaggat
     3721 cacgccttcg cgtgaggcga ggctgtactc gcgcaggaat gcatcgagcg gactgccgct
     3781 cgtactgccg gcgcgcaccc gctcgacc
//
LOCUS       DAOQVY010000167         3798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_27219_length_3798_cov_5.194764, whole genome shotgun sequence.
ACCESSION   DAOQVY010000167 DAOQVY010000000
VERSION     DAOQVY010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27219_length_3798_cov_5.194764"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /gene="gluQRS"
                     /locus_tag="PKN91_11755"
     CDS             complement(<1..763)
                     /gene="gluQRS"
                     /locus_tag="PKN91_11755"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HNS28783.1"
                     /translation="MTPCGRFAPSPTGDLHLGSLLAAAGSYLDARQAGGRWLLRIEDL
                     DTPRIVAGAEARILRTLEACGFEWDGAILHQADRRDAYRAALDTLARKGASFECSCSR
                     KDLLDAGGARVTGGGAYPGTCRQRPTRPGPTSTRLRVPDGSLSFDDRIQGPQHFAWSA
                     LGDVLIRRRDDIPAYQLAVVVDDAEQGVTDVVRGADLLDSTPWQIAIAQALGLAPPRY
                     AHLPVLVEPDGAKLSKSRRAVPVDPGRARRFLHTAL"
     gene            complement(760..1197)
                     /gene="dksA"
                     /locus_tag="PKN91_11760"
     CDS             complement(760..1197)
                     /gene="dksA"
                     /locus_tag="PKN91_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HNS28784.1"
                     /translation="MLAGPRNVQPYIPRRGEQYMGKAQLDHFREILLSWKHDLMMEVD
                     RTVSHMKDEAANFPDPNDRATQEEEFSLELRTRDRERKLIRKIDEALKRIEDGTYGYC
                     LETGEEIGIKRLEARPVATLSIEAQERRERRERQYGDRDDRYR"
     gene            complement(1584..1973)
                     /gene="queF"
                     /locus_tag="PKN91_11765"
     CDS             complement(1584..1973)
                     /gene="queF"
                     /locus_tag="PKN91_11765"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNS28785.1"
                     /translation="MPSQPSTTLETFANPQPDRDYTIRMTIPEFTCLCPKTGQPDFAT
                     LELEYVPDKLCVELKSLKLYIWSFRDRGAFHEAVTNEIVDTLAAATAPRFMRLAARFN
                     VRGGIYTTIVAERRQAGWAPAESVVLP"
     gene            2001..>3798
                     /locus_tag="PKN91_11770"
     CDS             2001..>3798
                     /locus_tag="PKN91_11770"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS28786.1"
                     /translation="MRLTKIKLAGFKSFVDPTAVSFPNNLTGVVGPNGCGKSNVIDAV
                     RWVMGELSARHLRGDSMTDVIFNGSSARKPVGTAHVELVFDNSDGKISGPYAAYSEVS
                     LKRQVSRDGTSTYFINGARCRRRDITQLFLGTGLGSRSYAIIEQGMISRVIESKAEDM
                     RAFVEEAAGISRYKERRRETENRIGDTRENLDRLQDLRDEIDKQIRHLQRQAATARRY
                     QAMKEDERRLTAEVLALRLRDLDSGAGVHDSAMRECDLTMQAALADQRAAEAAIDQQR
                     AFHNEQSELVTAVQGRYYEVGAEVSRTEQAIQHARELRERRTADLATARSSLGEVALL
                     IDRDAAELAALREEITALEPELEAATRAEHGAEGVLAEVEAALATWQAGWEEFNREFG
                     AADQATQVERARIEQLENQLRRLGGQADRLAVERDALLAQESEAQISDLAERESVARG
                     TAEQLAQALGAALASVQEARARQLQSETRLEALRTEREGLRGTMLSLEALQKAALERD
                     GGKAGDWIAASGLAGHGRLAGQLSVDGGWERAVETALGDYLEAVCVDDIDGVAGSMDS
                     LAGASVTLLESGRASGAGAIGGTLAQYVRGPAA"
BASE COUNT          600 a         1248 c         1357 g          593 t
ORIGIN      
        1 acagggcggt gtgcaggaac cggcgggccc tgccgggatc gacggggacc gcacggcgcg
       61 atttcgacag tttggcgcca tcgggttcga ccaggacggg caggtgcgca tacctcggcg
      121 gcgcaagccc cagggcctgt gcgatcgcga tctgccaggg cgtggagtcg agcaggtcgg
      181 cgccccgcac gacatcggtg acgccttgct ccgcatcgtc gaccaccacc gcgagctgat
      241 aggcgggaat gtcgtcgcgc ctgcggatca gcacgtcgcc gagcgcgctc caggcaaagt
      301 gctgcgggcc ctggatgcga tcgtcgaacg agaggctgcc gtcaggcacg cgcaggcggg
      361 tcgaggtcgg gccgggccgc gtgggccgtt gccggcaggt gccgggatag gcgccgccac
      421 ccgtgacgcg tgcgccgccg gcgtcaagga ggtccttgcg cgagcagctg cactcgaagc
      481 tcgcgccttt gcgtgccagc gtatcgagag ccgcacggta ggcatcgcgg cggtcggcct
      541 ggtgaaggat cgcgccgtcc cattcgaagc cgcaggcctc gagcgtgcgc aggatgcgcg
      601 cctcggcgcc agcgacaatg cggggtgtat cgaggtcctc gatgcgcagc agccagcggc
      661 cgccggcctg ccgcgcgtcg aggtagctac ccgctgcggc gagcagggaa ccgaggtgca
      721 gatcgccggt gggcgagggc gcgaagcgcc cgcatggtgt caacgatagc ggtcgtcgcg
      781 atcgccgtac tggcgctcgc ggcgctcgcg acgctcctgc gcctcgatcg acagggttgc
      841 gaccggacgg gcttccaggc gcttgatgcc gatctcctcg ccggtttcga ggcagtagcc
      901 gtaggtgcca tcctcgatgc gcttcagcgc ctcgtcgatc ttgcggatca gcttgcgctc
      961 gcgatcgcga gtgcgcagct cgaggctgaa ttcctcttcc tgcgtggcgc gatcgttcgg
     1021 atcggggaag ttcgcggcct catccttcat gtgggagacg gtgcgatcga cttccatcat
     1081 cagatcgtgc ttccagctga gcaggatctc acggaagtga tcgagctggg ccttgcccat
     1141 gtactgctcg ccgcgacgcg ggatatacgg ctgcacattg cgcgggccgg caaggatatt
     1201 gccaccgttg tcgggcgcgt cggcgtcatc cggcgagtcg gggaaatgcg gtgcgacccg
     1261 gatctgctcc gccgcgggct cgggcgcatg gcgcacgaag ctcgtctcga gcggtgcgac
     1321 cggtttcacg gtgctcttcg tggcgggcga tttcttcgcg ggcttcgggg cgtcgaccct
     1381 ggcacgcgcg cccgtcttgc gggctgcggg tttcgctgcg gtacgggtcg cagacctggt
     1441 ggcggtcttc ttcggcttgc tgctcttcgc aggcgccttc ttggcaggca tcctgagctc
     1501 ctttggaatc gcgggctcga aaacggacgc gcgattatac tggcgtcccg gcgcatgtac
     1561 agggccgcgt aagtgctgga ttttcagggc aggacgaccg actccgccgg tgcccagccc
     1621 gcctgtcggc gttctgcgac gatcgtcgtg taaatgcctc ccctgacgtt gaaacgcgcc
     1681 gcaaggcgca tgaagcgcgg cgccgtcgcc gcggcgaggg tatcgacgat ctcgttggtg
     1741 acggcctcgt ggaacgcacc gcgatcgcgg aacgaccaga tataaagttt gagcgatttc
     1801 aactcgacac agagtttgtc ggggacgtat tcgagctcca gcgtcgcgaa gtcgggctgg
     1861 ccggtcttcg ggcacagaca ggtgaattcc gggatcgtca tgcggatcgt gtagtcgcga
     1921 tccggctgcg gattcgcgaa ggtttcgagc gtcgtggagg gctgtgaagg catggcgatt
     1981 aatttacact agccgccgaa atgcgactaa ccaagatcaa gcttgccggc ttcaagtcct
     2041 tcgtcgatcc cactgcagtc agcttcccga acaacctgac gggcgtcgtc gggccgaacg
     2101 gctgcggcaa gtcgaacgtc atcgacgccg tgcgctgggt catgggtgag ctgtcggcgc
     2161 gccacctgcg cggcgactcg atgaccgacg tcatcttcaa cggttcgagc gcgcgcaagc
     2221 ccgtcggcac cgcgcacgtg gaactcgtgt tcgacaactc ggacggcaag atctccggcc
     2281 cgtacgccgc ctacagcgag gtgtccctga agcgccaggt ttcgcgcgac ggcacctcca
     2341 cctatttcat caatggcgcg cgctgccgcc gccgcgacat cacccagctg ttcctcggca
     2401 ccggcctcgg ttcgcgcagc tacgcgatca tcgagcaggg catgatctcg cgcgtcatcg
     2461 agtccaaggc cgaggacatg cgcgccttcg tcgaggaggc cgccggcatc tcgcgctaca
     2521 aggaacggcg gcgcgaaacc gagaaccgca tcggcgacac ccgcgagaac ctcgatcgcc
     2581 tgcaggacct gcgcgacgag atcgacaagc agatccgcca cctgcagcgc caggcggcca
     2641 cggcgcgccg ctaccaggcg atgaaggagg acgagcgcag gctcaccgcc gaggtgctgg
     2701 cgctgcgtct gcgcgacctc gacagcggcg cgggcgtgca cgacagtgcg atgcgcgagt
     2761 gcgacctcac gatgcaggcg gcgctcgcgg accagcgtgc ggccgaggcg gcgatcgacc
     2821 agcagcgcgc gttccacaat gaacagtcgg aactggtcac cgccgtacag ggccgctact
     2881 acgaggtcgg cgccgaagtc tcccgtaccg aacaggcgat ccagcacgca cgcgagctgc
     2941 gtgagcggcg caccgccgat ctcgcaaccg cacgcagctc gctcggcgag gtcgcgctgc
     3001 tgatcgatcg cgacgcggcc gaactcgcgg cgctgcgcga ggaaatcacc gcgctcgagc
     3061 cggagctcga ggccgccacc cgcgccgagc atggcgccga aggcgtcctc gccgaggtcg
     3121 aggcggcgct cgccacctgg caggccgggt gggaggaatt caatcgggag ttcggtgccg
     3181 ccgaccaggc gacccaggtc gagcgagcgc gcatcgagca gctggaaaac cagttgcgcc
     3241 ggctgggcgg gcaggccgac cggctcgccg tcgagcgcga cgcactgctc gcacaggagt
     3301 cggaggcaca gatttccgat ctcgccgaac gcgagtccgt ggcgcgcggc accgccgagc
     3361 agctcgcgca ggcgctgggg gcggcgctcg ccagcgtgca ggaagcgcgt gcccggcaat
     3421 tgcagagcga aacgcgcctc gaggccctgc gtactgagcg cgagggactg cgcggcacga
     3481 tgctgtcgct cgaggcactg cagaaagcgg cgctcgagcg cgacggcggc aaggccggcg
     3541 actggattgc cgcgtcggga cttgcggggc acggtcggct tgccggccag ttgtcggtcg
     3601 acggcggctg ggagcgcgcg gtcgagaccg cgctcggcga ctatctcgaa gccgtctgtg
     3661 tcgacgacat cgatggcgtc gccggctcga tggactcgct cgccggcgcc agcgtcacct
     3721 tgctcgagtc cggccgcgcg agcggggcgg gcgcgatcgg cggcacgctt gcgcagtatg
     3781 tgcgcggccc cgccgcgc
//
LOCUS       DAOQVY010000168         3645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_28893_length_3645_cov_5.433426, whole genome shotgun sequence.
ACCESSION   DAOQVY010000168 DAOQVY010000000
VERSION     DAOQVY010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28893_length_3645_cov_5.433426"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="PKN91_11775"
     CDS             <1..1000
                     /locus_tag="PKN91_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28787.1"
                     /translation="LATLHLPQDTLGRLAKMGVNGIGAALLLLGAGFARRFGHETLAT
                     LDRLVGREAAPRRAVVPRERYRARGEPSYELTSQPALVAFLAPMLEALEDFLRRRQGS
                     ITALECRLHHRGGAVTRCRLRFAQPAFAAAPIGFLLGERLAATTLAAPVTACELVSSR
                     LVPRRSASATLWQPGEHGGGPGSEAPELIERLRARLGEESVYGLCLVPEHRPEAAWRI
                     AEPLVHRNAGAGARQAASAGAAASAVPAIGADRRPLWLLSTPLPLPSPAGGNAWPHYE
                     GPLEKLAGPERIETGWWDDRDVARDYYVVRSGAAALLWIYRERRPPHAWFLHGVFG"
     gene            1032..1379
                     /locus_tag="PKN91_11780"
     CDS             1032..1379
                     /locus_tag="PKN91_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNS28788.1"
                     /translation="MIHQGSCHCGRIAFEADTDIGTVIDCNCSHCARKGYLLHFMPRE
                     KLRLTTPEANLSTWTFNKHAIRHRFCEVCGCAPFGEGVGPKGEAVVALNVRCIPSLDL
                     ATLEVKKVDGRSL"
     gene            1394..>3645
                     /locus_tag="PKN91_11785"
     CDS             1394..>3645
                     /locus_tag="PKN91_11785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HNS28789.1"
                     /translation="MTMRVPVQGGGAAPAYAELHCLSNFTFLRGASHPQELVERAAAL
                     GYAALAITDECSVAGAVRAHLAARQCGLPLLIGAEFHLRCGLAFVALATGRQGYARLC
                     RLITRGRRAAEKGRYDLGFDDVDALLEHCLVLWLPPRDLADETRAVALAGRIRERFAG
                     EAWLAVELLREGDDAARLESLMRIGRRAGLPLVASGDVHMHARARRRLQDAVTAIRLG
                     VPVAQAGHALYRNGERHLRERGRLAKLYPAPLLAATVEIAARCRFSLEELRYEYPREL
                     VPAGETASSWLRQLTEAGMRERWPQGAPQKVRDRVGHELTLIAELHYEPYFLTVHDIV
                     RYARGQGILCQGRGSAANSAVCYCLGVTAVDPAHEEMQLLMERFVSRERKEPPDIDID
                     FEHERREEVIQYIYGKYGRERTALTATVISYRPRSALRDLGKALGLDPAGIERLTRTL
                     QWWDREAGRVARASEAGFDPESPVLTRLFELAQELIGFPRHLSQHVGGFVISAGALEE
                     LVPIENAAMPDRTVIEWDKDDLDALGLLKVDVLGLGMLSAIRRALASVSALRGEPLTL
                     ADVPQDDRGVYDMLCRADAVGVFQVESRAQMAMLPRLKPRRYYDLVIQVAIVRPGPIQ
                     GGMVHPYLRRREGSEPVDYPGPEVEKVLKRTLGIPLFQEQVMQLAIEAAGFTPGEADQ
                     LRRSMAAWKRHGGLGHFHDKLTRGLLERGYGIEFAERIFKQILGFGEYGFPESHAASF
                     ALLVYVSAWL"
BASE COUNT          510 a         1248 c         1308 g          579 t
ORIGIN      
        1 gctcgccacg ctgcacctgc cgcaggacac actcggacgg ctcgcgaaga tgggcgtgaa
       61 cgggatcggg gccgcgctgc tcctgctcgg cgccggcttc gcgcgccgct tcgggcatga
      121 aaccctcgct acactcgacc gtctcgtcgg ccgcgaagcc gcaccgcgcc gcgccgtggt
      181 cccgcgcgag cgttatcgcg cgcggggcga gccctcgtac gagctcacga gccagcccgc
      241 cctggttgcg ttccttgctc cgatgctcga ggcgctcgag gatttcctgc gccgccgcca
      301 gggcagtatc acggcgctcg aatgccgcct gcaccaccgc ggtggcgccg tcacgcgctg
      361 ccgcctgcgc tttgcgcagc cggccttcgc cgcggcaccg atcggtttcc tgctcggcga
      421 gcgcctcgcc gcgaccacgc tggcagcacc ggtcacggcc tgtgaactcg tgagcagccg
      481 gctcgtgccg cggcggtccg cgagcgcgac cctatggcag ccgggagaac acggcggcgg
      541 gcccgggagc gaagcgcccg aactcatcga gcgcctgcgc gcgcggctcg gcgaggaaag
      601 tgtctacgga ctttgcctcg tgccggagca tcggcccgag gcggcgtggc gcatcgccga
      661 gccgctcgtg catcgcaatg ccggcgcagg tgcgcggcag gctgccagcg cgggcgccgc
      721 ggcatcggcg gtgccggcga tcggcgcgga ccggcgcccg ctgtggctgc tgtcgacgcc
      781 attgccgctg ccgtcaccgg caggcggcaa tgcctggccg cattacgagg gtccgctcga
      841 gaagctcgcg ggtcccgaac gtatcgagac cggctggtgg gacgaccgcg atgtcgcgcg
      901 cgactactac gtcgtgagga gcggcgcggc ggcgctgctg tggatctacc gggagcgcag
      961 gccgccgcat gcgtggttcc tgcacggagt gtttggttga ccgtctggtc attcacgcga
     1021 ggagcatcga catgatccat caaggcagtt gtcattgcgg ccggatcgcc ttcgaggccg
     1081 ataccgacat cggcaccgtc atcgactgca actgcagtca ctgcgcacgc aagggttatc
     1141 tgctgcattt catgccgcgc gagaaactgc gtctcaccac gccggaggcg aatctctcga
     1201 cctggacctt caacaagcac gcgatcaggc accgcttctg cgaagtctgc ggttgtgccc
     1261 cgttcggcga aggcgtcggc ccgaagggcg aggcggtcgt ggcgctcaat gtgcgctgca
     1321 tcccgtccct cgaccttgcc acgctcgaag tgaagaaagt cgacgggcgc agcctctagc
     1381 gcggcgcgac ggcatgacga tgcgcgttcc ggtgcagggc ggcggcgcgg ccccggccta
     1441 cgccgagctg cactgcctct ccaacttcac ctttctgcgc ggcgcctcgc atccgcagga
     1501 actggtcgag cgcgccgccg ccctcggcta tgcagcgctc gcgatcaccg acgagtgctc
     1561 ggtcgccggg gccgtgcgcg cgcatcttgc ggcgaggcag tgcgggttgc cgctgctcat
     1621 cggcgcggag ttccacctgc ggtgtgggct tgcgttcgtc gcgctcgcga ccggccgcca
     1681 gggctatgcg cgtctctgcc ggctgatcac gcgcgggcgg cgcgcggcgg agaagggccg
     1741 ctacgatctc ggtttcgatg acgtcgatgc gctcctcgaa cactgtctcg tgctgtggct
     1801 gccgccgcgg gacctggccg acgagacgcg cgcggtggcg ctggccgggc gcatccgcga
     1861 acgtttcgcc ggcgaggcct ggctcgcggt cgagctgctg cgcgaaggcg acgatgccgc
     1921 gcgcctcgag tcgctcatgc ggatcggccg gcgcgcgggg ctgccgctcg tcgcgagtgg
     1981 cgacgtgcac atgcatgcgc gcgcgcgccg gcgcctgcag gatgccgtga ccgcgatccg
     2041 gctcggcgtg ccggtcgcgc aggcgggcca tgcgctgtat cgcaacggcg agcgccatct
     2101 gcgcgagcgc ggccggctcg cgaagctcta tcccgcgccg ctgctcgcgg cgaccgtgga
     2161 gatcgccgcg cgctgccgtt tctcgctcga ggaactgcgc tacgagtatc cgcgggaact
     2221 cgtgccggcc ggcgaaacgg cgtcatcctg gctgcgccag ctcaccgagg cgggcatgcg
     2281 cgaacgctgg ccgcagggcg cgccgcagaa ggtgcgcgac cgggtcgggc acgagctcac
     2341 gttgattgcc gagctgcatt acgaacccta tttcctcacg gtgcacgaca tcgtccgcta
     2401 cgcgaggggg cagggcatcc tgtgccaggg tcgcggctcg gcggccaatt cggcggtgtg
     2461 ctattgcctc ggcgttaccg cggtcgatcc ggcgcacgag gaaatgcagc tgctgatgga
     2521 gcgcttcgtc tcgcgcgagc gcaaggagcc gcccgacatc gacatcgact tcgagcatga
     2581 acggcgcgag gaggtgatcc agtacatcta cggcaagtat ggccgcgagc gcacggcgct
     2641 gaccgcgacc gtgatcagtt atcgaccgcg cagcgcgctg cgcgatctcg gcaaggcgct
     2701 gggcctcgat ccggcgggta tcgagcgcct cacccgcacg ctgcagtggt gggatcgcga
     2761 ggcggggcgg gtcgcacgcg cgagcgaggc ggggttcgat ccggaaagcc ccgtgctcac
     2821 gcgactgttc gagctcgcgc aggagctgat cggctttccg cgccatctct cgcagcatgt
     2881 cggcggcttc gtgatctcgg cgggagcgct cgaggaactg gtgccgatcg agaacgccgc
     2941 gatgcccgat cgcacggtga tcgaatggga caaggacgac ctcgatgcgc tcggcctgct
     3001 caaggtggat gtgctgggcc tcggcatgct ttcggcgata cggcgcgcgc tggcgtcggt
     3061 ctcggcgctg cggggtgagc cgctcacgct ggccgatgtc ccgcaggacg accggggcgt
     3121 ctacgacatg ctctgccgcg ccgatgcggt cggcgtgttc caggtggaat cgcgcgcgca
     3181 gatggcgatg ctgccgcgcc tgaagccgcg ccgttactac gacctcgtga tccaggtggc
     3241 gatcgtgcgg ccggggccga tccagggcgg catggtgcat ccctatctgc gccggcgcga
     3301 aggcagcgag ccggtggact atccgggccc cgaagtcgag aaggtgctca agcgcacgct
     3361 cggcatcccg ctgttccagg aacaggtgat gcagctcgcg atcgaggctg cgggcttcac
     3421 gccgggcgag gcggaccagc tgcgccgctc gatggcggcc tggaagcgcc acggcgggct
     3481 cggtcatttc cacgacaagc tgacccgcgg cctgctcgag cgaggctacg gcatcgagtt
     3541 cgcggaacgg atcttcaagc agatcctcgg cttcggcgaa tacggctttc ccgaatcgca
     3601 tgccgcgagc ttcgcgctgc tcgtgtatgt ctccgcctgg ctcaa
//
LOCUS       DAOQVY010000169         3632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_29037_length_3632_cov_5.730221, whole genome shotgun sequence.
ACCESSION   DAOQVY010000169 DAOQVY010000000
VERSION     DAOQVY010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29037_length_3632_cov_5.730221"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..106
                     /locus_tag="PKN91_11790"
     CDS             <1..106
                     /locus_tag="PKN91_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016141872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNS28790.1"
                     /translation="LSRLTLPHALVRVVLAEQLYRAHTVLAGHPYHRA"
     gene            103..723
                     /locus_tag="PKN91_11795"
     CDS             103..723
                     /locus_tag="PKN91_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HNS28791.1"
                     /translation="MTAGAPVLCLASASPRRRELLHQIGVSHHVLVTDVDESPRAGES
                     PADYVARVALGKARAAWRDPQARRHLPVLAADTTVVLDGRMLGKPATRADCVAMLQDL
                     SGRTHEVLTAITLLAGSAEAPVVRARVSRSEVRFRGIDAPEAGRYWDTGEPRDKAGGY
                     AIQGRGAVFVAGLAGSYSGVMGLPLFETAGLLGEAGIPVWTGRPAP"
     gene            720..2192
                     /gene="rng"
                     /locus_tag="PKN91_11800"
     CDS             720..2192
                     /gene="rng"
                     /locus_tag="PKN91_11800"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HNS28792.1"
                     /translation="MTTEILINSGLRETRAALVENGALQEVHIERASRRGLVGNIYKG
                     RVTRVLPGMQAAFIDVGLERAAFLHVDDIAVTRPDSTMVGLPKIDDIRRLVNQGDDLL
                     VQIVKDPLGTKGARLTTFLSLPSRFLVYMPRSDGNVGVSARIEDEAERQRLRELIADL
                     PQPAQGGGFILRTAAAGVTRDALRADMIYLGRLWDHVRERALAAAPGALVHEDLPLPT
                     RMLRDELGRDIERVLVDAPAAFGEMQAFARAFMPEAAGRIELYQGQRPIFDLHGIEDE
                     IARALGRKVELKSGGHVVIDQTESMTTIDVNTGAYVGHRNLEETIFRTNLEAAVAIAR
                     QLRLRNLGGIIIIDFIDMQDESHRRQVLEALSRSLAADRVQTHIASLSPLGLVEMTRK
                     RTRESLEHLLCQPCPTCEGRGFVRTPETVCFEILREILRQARQFGSRELVILAHQDVI
                     GRLLDEEAPALSELETANGKPIRLQVEPLYGVDQYDVVLT"
     gene            2189..3007
                     /locus_tag="PKN91_11805"
     CDS             2189..3007
                     /locus_tag="PKN91_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNS28793.1"
                     /translation="MSAPRVAAIQMTTSADVGANLAQAAHWLAQAADRGAVVAALPEN
                     FAFMGLADADKRAIAEEPGAGPIQDFLADAALRHSMMVIGGTVPLRVVGGDGRVAAAC
                     LVFDTGGRAVARYDKIHLFDVELPAAGESYLESAHVAPGHAPQVIDTPAGRLGLAVCY
                     DVRFPELFRRLVAGGAEWFVLPSAFTVPTGQAHWETLLRARAIENLAFMVAPAQSGRH
                     ANGRLTHGNTLICDFWGRILGRLESGPGCVVAEFDRAAQADARARFPALRHRVL"
     gene            3024..>3632
                     /locus_tag="PKN91_11810"
     CDS             3024..>3632
                     /locus_tag="PKN91_11810"
                     /inference="COORDINATES: protein motif:HMM:NF040244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HNS28794.1"
                     /translation="MADETQPLAIARELILRPGGLDVARIDRVLGEVMGHAVDAADVY
                     FQLSREESWALEDGIVKEGGASIEQGVGVRALAGEKTGFAYSDEVVLPALEEAGRAAR
                     AIAAGGAQHALRVTHPASGHRLYLPIDPGAALTDRDKVGCLERVDREIRKSDPRIVQV
                     MASVSSVYEVILVATSDGTLAADVRPLVRFNVSVVAEQNGRRE"
BASE COUNT          513 a         1260 c         1316 g          543 t
ORIGIN      
        1 gctgtcccgg ctcacgctgc cgcacgctct cgtgcgcgtg gtgctcgccg agcagctgta
       61 tcgcgcgcat accgtgctcg ccggccaccc gtaccaccgc gcatgacggc cggcgcaccc
      121 gtgctgtgcc tcgcctcggc ctcgccgcgg cggcgcgaac tgctgcacca gatcggggta
      181 tcgcaccacg tgctcgtgac cgatgtcgac gagtccccgc gggcgggcga gtcgccggcc
      241 gattacgtcg cccgcgtggc gctcggcaag gcgcgcgcgg cctggcggga cccgcaggcg
      301 cgccggcacc tgccggtgct ggcggccgat acgaccgtgg tgctggatgg gcgcatgctc
      361 ggcaagcccg cgacgcgcgc ggactgcgtc gcgatgctgc aggacctctc aggacgcacg
      421 cacgaggtgc tcacggcgat cacgctgctg gctggctccg ccgaggcgcc ggtcgtcagg
      481 gcccgcgtga gccgctcgga ggtgcgcttt cgcggcatcg atgcgcccga ggccggccgt
      541 tactgggaca ccggcgagcc gcgcgacaag gccggcggct acgcgatcca ggggcgtggc
      601 gcggtattcg tcgccggcct cgcgggcagc tattctggcg tcatgggatt gccactattc
      661 gagactgccg ggctgctggg cgaggccggg atcccggtct ggaccgggcg gccggcgccg
      721 tgacgaccga gatcctgatc aacagcggac tgcgcgagac ccgcgccgcg ctcgtcgaga
      781 acggtgcgct gcaggaagtg cacatcgagc gggcgagccg ccgcggcctc gtcggcaaca
      841 tctacaaggg gcgcgtgacg cgcgtgctgc cggggatgca ggccgcattc atcgacgtcg
      901 ggctcgagcg cgcggccttc ctgcacgtcg acgacatcgc ggtgacgcgg cccgactcga
      961 cgatggtggg cctgccgaag atcgacgaca tccgccggct cgtgaaccag ggcgacgatc
     1021 tgctggtgca gatcgtcaag gatccgctcg gcaccaaggg tgcgcggctc accacgttcc
     1081 tgtcgctgcc gtcgcgtttt ctcgtctaca tgccgcgcag cgacggtaac gtgggcgtgt
     1141 cggcgcgcat cgaggacgag gccgagcgcc agcggctgcg cgaactcatc gccgacctgc
     1201 cgcagcccgc gcagggcggc ggcttcatcc tgcgcacggc ggccgcgggc gtgacgcgcg
     1261 atgcgctgcg cgccgacatg atctacctcg gccgcctgtg ggaccacgtg cgcgagcgcg
     1321 cgctggcggc cgcgcccggc gccctcgtgc acgaggacct gccgctgccg acgcgcatgc
     1381 tgcgcgacga actcgggcgc gacatcgagc gcgtcctggt cgatgcgccg gcggcgttcg
     1441 gcgagatgca ggcgttcgcg cgcgccttca tgccggaggc cgcgggccgc atcgaactct
     1501 accagggcca gcgcccgatc ttcgacctgc acggcatcga ggacgagatc gcgcgcgcgc
     1561 tgggccgcaa ggtcgaactc aagtccggcg gccacgtggt catcgaccag accgagtcga
     1621 tgaccacgat cgacgtgaac accggcgcct atgtcgggca ccgcaacctc gaggagacga
     1681 ttttccgcac caacctcgag gcggcagtgg cgatcgcgcg gcagctgcgg ctgcgcaacc
     1741 tcggcggcat catcatcatc gatttcatcg acatgcagga cgagtcgcac cgccgccagg
     1801 tgctcgaggc gctctcgcgc agcctcgcgg ccgatcgtgt gcagactcac atcgcgagcc
     1861 tgtcgccgct cggcctcgtc gagatgaccc gcaagcgcac gcgcgagagc ctcgaacatc
     1921 tgctgtgcca gccgtgcccg acctgcgagg gtcgcggctt cgtgcgcacg ccggaaaccg
     1981 tgtgtttcga gatcctgcgg gagatcctgc gccaggcgcg gcagttcggc agccgcgagc
     2041 tcgtgatcct cgcgcaccag gacgtgatcg ggcgcctgct cgacgaggag gcgccggcgt
     2101 tgtccgaact cgagacggcc aacggcaagc ccatccggct gcaggtcgaa ccgctctacg
     2161 gcgttgatca gtacgacgtg gtgctcactt gagcgcgccg cgcgtcgcgg cgatccagat
     2221 gaccacgtcg gccgatgtcg gcgcgaacct ggcgcaggcc gcgcactggc ttgcgcaggc
     2281 ggccgatcgc ggtgccgtgg tggcggcact gcccgagaat ttcgcgttca tgggcctggc
     2341 cgatgccgac aagcgcgcga tcgccgagga gccaggggcc gggcccatcc aggatttcct
     2401 ggccgacgcg gcgctgcgcc acagcatgat ggtgatcggc ggcacggtgc cgctgcgcgt
     2461 cgtcggcggc gacggccggg tcgccgcggc ctgcctcgtt ttcgataccg gcgggcgcgc
     2521 cgttgcgcgc tacgacaaga tccacctgtt cgacgtggaa ctgcccgcgg cgggcgagag
     2581 ctacctcgag tcggcgcacg tggcgcccgg gcatgcgccg caggtgatcg acacgcccgc
     2641 cggccggttg gggctcgccg tgtgctacga tgtgcgcttc cccgagctct ttcgtcgcct
     2701 cgtcgcaggg ggtgcggaat ggttcgtact gccctcggcg ttcaccgtgc ccaccggcca
     2761 ggcgcactgg gaaaccctgc tgcgggcgcg tgccatcgag aacctcgcgt tcatggtggc
     2821 gccggcgcag tcgggccggc acgcgaatgg gcgactgacc catgggaata cactgatctg
     2881 cgatttctgg gggcgcatac tcgggcggct ggagtcgggt ccgggttgcg tagtggcgga
     2941 gttcgatcgc gcggcgcagg ccgatgcgcg cgcccggttt cccgcattgc gacaccgcgt
     3001 cctgtagcga gctgcacacc aacatggctg acgagaccca acccctggcg attgcgcgcg
     3061 agctgatcct gcggcccggc gggctcgacg tcgcacgcat cgatcgcgtg ctcggcgagg
     3121 tcatgggaca cgcggtcgat gccgcggatg tctatttcca gctctcccgc gaggagtcgt
     3181 gggcgctcga ggacggcatc gtcaaggaag gcggcgcgag catcgagcag ggcgtgggcg
     3241 tgcgtgcgct cgccggcgag aagaccggct ttgcgtattc ggacgaagtg gtgctgccgg
     3301 cgctcgagga agccggccgc gcggcgcgtg cgatcgccgc gggcggcgcg cagcatgcgc
     3361 tgcgcgttac ccacccggcg tccggccaca gactgtacct gcccatcgat ccgggtgcgg
     3421 cgctcaccga tcgcgacaag gtcggctgcc tcgagcgcgt ggaccgcgag atccgaaaat
     3481 ctgatccgcg catcgtgcag gtgatggcga gcgtgtcctc ggtctacgag gtcatcctgg
     3541 tcgcgaccag cgacggcacg ctcgcggccg acgtacgccc gctggtgcgt ttcaacgtct
     3601 cggtggtcgc cgagcagaac ggccgccgcg aa
//
LOCUS       DAOQVY010000170         3621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_29156_length_3621_cov_6.715087, whole genome shotgun sequence.
ACCESSION   DAOQVY010000170 DAOQVY010000000
VERSION     DAOQVY010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29156_length_3621_cov_6.715087"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PKN91_11815"
     CDS             <1..478
                     /locus_tag="PKN91_11815"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNS28795.1"
                     /translation="LRDADAALARLRQRMRLASPGVRVAQAVQRLDELEARLALAMRG
                     ALLGQRHRAAELGRRLVEASPVARVHAAAASGKALAIRLERAMLGELARGAHRLDLAS
                     RTLDAVSPLATLQRGFAVVVRATDRSLVTDAATLKVGESLITRVASGEFRSRVTED"
     gene            478..1356
                     /locus_tag="PKN91_11820"
     CDS             478..1356
                     /locus_tag="PKN91_11820"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNS28796.1"
                     /translation="MSPIPKLLLACAALALFTPVRAATAAADPALPRASAVPGGVLLI
                     DVGALAAHDGAAPVVTLDGVRTMVVPREGQWTAVVGVPLSQAAPATLELRVQYRDGSE
                     RRISTAIADKAYAAQHLKVKPGQVNLSKADLARTSAERERIQAALATWSPAPPATLEL
                     RQPVPGPRSSSYGLRRFFNGEARNPHTGMDIAAPTGTPIRAPADGLVIDTGDYFFNGN
                     TVLIDHGQGLVTMYCHLSAIEVEAAQRVKTGEQLGKVGATGRVTGPHLHWGVTLNRTS
                     VDPALFLPAATPATAP"
     gene            complement(1318..2733)
                     /gene="der"
                     /locus_tag="PKN91_11825"
     CDS             complement(1318..2733)
                     /gene="der"
                     /locus_tag="PKN91_11825"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNS28797.1"
                     /translation="MLPVVALVGRPNVGKSTLFNALTRTRDALVADVPGVTRDRQYGY
                     GKVGPAPYVVIDTGGIVEQPAGIEAPMRQQTERAVAEADVLVLIVDARSGITAQDEFI
                     ARLLRRAGKRVVLAANKAEGLDSDVATAEFHGLGFGEPVPLSAAHGQGARELMEVVLG
                     VPDAEAADDEDRVDAGAPVEPVPDVIRVAIIGRPNVGKSTLVNRLLGEERVIASDMPG
                     TTRDSIFVPFSRDGRNFELIDTAGVRRRAKIDDAIERASVSKTLQAIFEAHVVIVVLD
                     AHEGIGEQDASVLGLALERGRALLIAVNKWDNIPMERREEIRGQLALKLDFAPFVPLH
                     FISARHGTGVGELAQAVVRAFDAAMREMRTPELTRTLERAMTQHQPPLVRGRRIKLRY
                     AHQGGRNPPRIVIHGNQTKHVPDAYKRYLANVFRETFDLFASPVAIEFRTDANPYDRG
                     IRRPADRAPSGRRGRRRGRKE"
     gene            complement(2745..>3621)
                     /gene="bamB"
                     /locus_tag="PKN91_11830"
     CDS             complement(2745..>3621)
                     /gene="bamB"
                     /locus_tag="PKN91_11830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HNS28798.1"
                     /translation="QVKTKARLTGGTGADAALVAVGTGGGEVIALSAVDGRQLWRIAV
                     RGEVLSAPLVTPAAVVVRTVDGRLVALARDDGHELWNVEQQIPRLTLRGAAAPQFSGD
                     TVVCGFDNGKVVATSLRDGTVMWETAVAPPRGRTELERLSDIDGSVRIAGEDIYAVGF
                     QGRVAMLALASGQIWWSRDLSSYRGLALDDENVYVSSSEGDVLSMQRRTGAEGWRQST
                     LAYRGLSAPAAIGAHVAVGDFEGYVHFLDKANGEIVARVKAGGARISTPPVVAGDMLV
                     VIDDDGRMSALRPRT"
BASE COUNT          532 a         1339 c         1217 g          533 t
ORIGIN      
        1 gctgcgcgat gcggacgccg cgctcgcgcg tctgcgccag cgcatgcgcc tcgcctcacc
       61 cggtgttcgc gtggcgcagg ccgtacagcg cctggatgag ctcgaggcgc gcctcgcgct
      121 tgcgatgcgc ggcgcgctgc tcggccagcg ccatcgcgcc gcggaactcg gccggcgcct
      181 cgtcgaggcc tcgccggtgg cgcgcgtgca cgccgcggcc gcctccggca aggcattggc
      241 gatccgcctc gagcgcgcaa tgctcggcga gcttgcgcgc ggcgcccatc ggctcgatct
      301 cgcgagccgc acgctcgacg ccgtgagccc gctcgccacc ctgcaacgcg ggtttgccgt
      361 cgtcgtgcgt gcgaccgacc ggtcgctggt cacggacgcc gcgacgctca aggtcgggga
      421 gtcactgata acacgggtcg cgtcgggcga gttccgctcg cgcgtgacgg aggactgatg
      481 tcgcccatcc cgaagttact gctggcctgc gcggccctcg cgctgttcac gccggtacgc
      541 gccgcgacag ctgcggcgga tccggcactg ccccgggcga gcgccgtgcc cggcggcgta
      601 ttgctcatcg atgtcggcgc gctcgcggca cacgacggcg ccgcgccggt cgtcacgctg
      661 gacggcgtgc gcacgatggt cgtgccgcgc gagggccagt ggaccgcggt ggtcggcgtg
      721 ccgctgtcgc aggcggcgcc tgcgaccctc gagctgcgcg tgcagtaccg cgacggcagc
      781 gagcgacgca tcagcaccgc catcgcggac aaggcctacg cggcccagca cctcaaagtg
      841 aaacccggcc aggtcaatct gtcgaaggcg gacctcgcgc gcaccagcgc ggagcgtgag
      901 cgcatccagg ccgcgctcgc cacctggtcg ccggcaccgc ccgccacgct cgagctgcgc
      961 cagccggtac cggggccgcg ctcgagctcc tacggcctgc ggcgcttctt caatggcgag
     1021 gcccgcaatc cgcacaccgg catggacatc gcagcgccca ccggcacacc gatccgcgct
     1081 cctgccgacg gactcgtgat cgacacgggc gattacttct tcaacggcaa tacggtcctg
     1141 atcgatcacg gccaggggct cgtcacgatg tactgccacc tgtccgcgat cgaggtcgag
     1201 gcggcccagc gcgtgaagac gggcgagcag ctcggcaagg tcggggcgac cggtcgcgtc
     1261 accggacccc atctgcattg gggtgtgacg ctcaaccgca cttcggtcga tcctgcgcta
     1321 ttccttccgg ccgcgacgcc ggccacggcg ccctgacggc gcgcggtcgg cgggtcgccg
     1381 gatcccgcgg tcgtagggat tcgcatcggt gcggaattcg atcgccaccg gcgaagcaaa
     1441 caggtcgaag gtctcgcgaa acacattggc gagatagcgc ttgtacgcgt ccggcacgtg
     1501 cttggtctgg ttgccgtgga tcacgatgcg cggcggattg cgcccgccct ggtgcgcata
     1561 gcgcagcttg atgcgccggc cgcgcacgag cggcggctgg tgctgcgtca tcgcgcgctc
     1621 gagggtcctc gtgagttcgg gcgtgcgcat ttcgcgcatc gcggcgtcga acgcacggac
     1681 cacggcctgc gccagttcgc ccaccccggt accgtggcgc gccgatatga agtgcagcgg
     1741 cacgaaaggg gcgaaatcga gcttgagcgc gagctggccc cggatctcct cgcgtcgctc
     1801 catggggatg ttgtcccact tgttgacggc gatcagcagc gcgcgcccgc gctcgagcgc
     1861 gagcccgagc acgctcgcgt cctgctcgcc gatgccctcg tgcgcatcga gcaccacgat
     1921 gacgacgtgc gcctcgaata tcgcctgcag cgtcttcgag acgctggcgc gctcgatcgc
     1981 atcatcgatc ttcgcacgac ggcgcacgcc cgccgtgtcg atcagttcga agttccggcc
     2041 gtcgcgactg aagggcacga agatgctgtc gcgcgtcgta ccgggcatgt cgctcgcgat
     2101 cacgcgctct tcgccgagca gccggttgac gagcgtcgac ttgccgacgt tcggccggcc
     2161 gatgatcgcc acgcgaatga catccggcac gggttcgacc ggtgccccgg catcgacgcg
     2221 atcctcgtcg tccgcagcct cggcatccgg cacgccgagc accacttcca tcagctcgcg
     2281 agcgccctgg ccatgcgcgg ccgacaacgg caccggctca ccgaagccga gcccgtgaaa
     2341 ctcggcggtc gcgacgtcgc tgtcgaggcc ttcggccttg ttcgcggcaa gcaccacgcg
     2401 cttgccggca cgacgcagca gccgcgcgat gaactcgtcc tgcgcggtga tgccggagcg
     2461 cgcatcgacg atgagcacga gcacgtcggc ctcggccacc gcacgctcgg tctgctggcg
     2521 catcggcgcc tcgatgccgg cgggctgctc gacgatgccg cccgtgtcga tcacgacata
     2581 cggcgcgggg ccgaccttgc cgtagccgta ctggcgatcg cgcgtgacgc cgggcacgtc
     2641 cgcgacgagc gcgtcgcgcg tgcgcgtcag cgcattgaaa agggtcgact tgccgacatt
     2701 cggtcggccg accagcgcga cgactggcag catgacatcg gctttcaggt tctcgggcgc
     2761 agcgcgctca tgcgcccatc gtcatcgatc acgaccagca tgtcacccgc gacgacgggt
     2821 ggcgtcgaaa tgcgcgcgcc gcccgccttc acgcgggcga cgatctcgcc attggccttg
     2881 tcgagaaagt gcacatagcc ctcgaagtcc ccgaccgcga cgtgcgcgcc gatggcggca
     2941 ggcgccgaca gcccccggta cgcgagcgtc gactgccgcc agccttccgc gccggtgcgg
     3001 cgctgcatgc tgagcacgtc cccctcggaa ctcgacacgt agacattctc atcgtctagc
     3061 gcgaggccgc gataactcga caggtcgcgc gaccaccaga tctgcccgga agcgagcgcc
     3121 agcatcgcga cacggccctg gaagcccacc gcgtagatgt cctcgcccgc aatccgcacg
     3181 ctcccgtcga tgtcgctcag gcgttcgagc tcggtacgtc cgcgcggtgg agccaccgcc
     3241 gtctcccaca tcaccgtgcc gtctcgcagg ctcgtcgcga ccaccttgcc gttgtcgaag
     3301 ccgcacacca cggtgtcgcc ggagaactgc ggtgcggctg cgccccgcag ggtgaggcgc
     3361 ggaatctgct gctcgacatt ccacagctca tggccgtcat cgcgcgcgag cgcgaccagg
     3421 cgaccatcga ccgtgcgcac aacgacggca gcgggcgtca cgagcggcgc ggacagcacc
     3481 tcaccgcgca cggcgatacg ccacagctgg cggccatcga cggcggacag cgcaatcact
     3541 tcgcccccgc ccgtgcccac cgccacgagc gcggcatcgg cgccggtgcc gccggtcagg
     3601 cgtgccttcg tcttcacctg c
//
LOCUS       DAOQVY010000171         3578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_29658_length_3578_cov_4.942663, whole genome shotgun sequence.
ACCESSION   DAOQVY010000171 DAOQVY010000000
VERSION     DAOQVY010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29658_length_3578_cov_4.942663"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2483)
                     /locus_tag="PKN91_11835"
     CDS             complement(<1..2483)
                     /locus_tag="PKN91_11835"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNS28799.1"
                     /translation="MSRTTDAVNVHAGGARSLPVLLALGGCFALSGLAALVYQTAWTR
                     EFALVFGTSELAVATVLAAYMGGLALGAALVDRLLPRIARPVLTYAVLELGIGLSAVV
                     LVPLLLLASSRLLDVLFGGQPAPPSSEHGALSWYYLASAFVVLAVPATLMGATLPLLA
                     RHAVHTEQEIGRRIGLLYAANTAGAVGGALLAAFWLLPTFGLRAAIWIAAAINGLVFV
                     IASGIARLAPARSRPDTATRTAPPTRDAAQWILPLMLLSGAVTFLNEVLWTRMLSHVV
                     GSSIHAFGVMVASFLTGIALGGGVAAWFATTRERSTRLFIACEFGVALSAAGAFLLVD
                     RYVPAQAGLAGNAFFGFFLLLPLTLFIGATYPFAVRMLAHGPDDAAAASARVYAWNTV
                     GAIFGAIMGGFLIIPALRYEGAIRGAVIASAALAAAAAWLLARRPRALPWFATAATLS
                     IALLFRADPPTRLLLTSPLDVPTEGELLFYEVGRSADVVVLGQDGGLALRTNGLPEAL
                     MDTPGIAPRFSGEFWMSSLAVLARPATERMLIVGYGGGVVVEGVPPSVRAIDVIELEP
                     VVIEANRATRQLRRRDPLLDTRVNIVTNDARGALRLTDRSYDAIVSQPSHPWTAGASH
                     LYTREFMQLAADHLTPGGVFVQWMNVSFLDEGLLRSLTATLLDVFGEVRIYRPDPSTL
                     VFLASDAPIDPETPLVGNGYPLNRAPLHYARYGLNCAEDLVAALVADAAGTRRLAAGA
                     PLITDERNRMATSSVLELARGMSADQTGRVLAEIDPLQRPDSAVHRDLLPRLSTSYIA
                     RRLSTHVAIDPSTRDRIARMGTLLGDTPRG"
     gene            2518..3324
                     /locus_tag="PKN91_11840"
     CDS             2518..3324
                     /locus_tag="PKN91_11840"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNS28800.1"
                     /translation="MQAPGVITITTDFGHKGPFVGVMKGRIYGRLPTARIVDLTHEIL
                     VHWPAEAGFWLTRAYQYFPTGTVHVAVVDPGVGTGRDIIAVSVDGHVFLAPDNGLLAP
                     VVGAREDARIVKVDLQALARFGVTRPSATFHGRDIFAPLAAELAAGRCRPEDLGSPVD
                     VLVPSWVEEASGDESAVHGVVITLDHFGNLITNIDAHWVVRMGSPRVYAANHSWPLLR
                     TYGDVKPGEYLALVNSFGVVEIARAEQSAAEGLGLSRGAPVVVRDTARLP"
BASE COUNT          619 a         1149 c         1293 g          517 t
ORIGIN      
        1 ccgcgcggcg tatcgccgag caacgtcccc atgcgtgcga tgcgatcgcg agtgctggga
       61 tcgatggcga cgtgagtcga gaggcgccgg gcgatgtaac tggtcgacag gcgcggcagg
      121 aggtcccggt gcacggcgct gtccgggcgc tgcagcggat cgatctccgc gagtacgcgg
      181 cctgtctggt ccgcactcat gccccgggcc agctcgagca cgctggaggt ggccatgcgg
      241 ttgcgctcgt cagtgatcag cggcgcgccg gccgcgagtc gccgcgttcc ggccgcgtcc
      301 gccaccagcg cggcaacgag gtcttccgcg cagttgaggc catagcgtgc gtagtgcagc
      361 ggcgcgcggt tcagcggata accgttgccg acaaggggcg tttccgggtc gataggcgcg
      421 tccgaggcga ggaacacgag tgtgctggga tcggggcggt agatgcgcac ttccccgaac
      481 acatcgagca acgtcgcggt gagcgagcgc agcagtccct cgtcgaggaa gctgacattc
      541 atccactgca cgaagacgcc accgggtgtc aggtgatcgg cggcgagctg catgaactcg
      601 cgcgtataga gatgcgaggc gccggcggtc cacgggtgcg acggctggga aacgatcgcg
      661 tcgtagcttc gatcggtgag ccgcagcgcg ccgcgtgcat cgttggtcac gatgttgacg
      721 cgggtgtcga gcagcgggtc gcggcgtcgc agctggcgtg tggcacggtt cgcctcgatc
      781 accacgggtt cgagttcgat gacgtcgatc gctcgcacgg acggcggcac gccctcgacg
      841 acgacaccgc cgccgtagcc tacgatcagc atgcgttcgg tcgccgggcg cgccagcacc
      901 gcgagtgagg acatccagaa ctcgccgctg aaacgcgggg ctatccccgg cgtatccatc
      961 agcgcctccg gcagcccatt cgtgcgcagc gcgaggccgc cgtcctggcc gagcacgacg
     1021 acgtcggcgc tgcggccgac ttcgtagaag aggagctcgc cttcggtcgg cacgtcgagc
     1081 ggcgaggtca gcagcagccg ggtcggcgga tcggcacgga acagcagtgc aatcgacagc
     1141 gtggcggccg tggcgaacca gggcagggca cgcgggcgtc gtgcgagcag ccaggcggcg
     1201 gcggcggcga gcgcagcgct cgcgatcacg gcgccgcgaa tggcgccctc gtaacgcagc
     1261 gcgggtatga tgaggaagcc gcccattatt gcgccgaaaa tggcgcccac cgtgttccat
     1321 gcatagacgc gcgcgctcgc ggcggcggca tcgtcggggc cgtgggcgag catgcgcacg
     1381 gcgaacggat aggtggcgcc gataaagagc gtgagcggca gcagcaggaa gaagccgaaa
     1441 aacgcattgc cggcgaggcc ggcctgggcg ggcacgtagc gatcgacgag caggaatgcc
     1501 cccgcggccg acagcgccac gccgaattcg caggcgatga acaggcgcgt cgagcgctca
     1561 cgggtggttg caaaccatgc ggcgacaccg ccgccgagcg cgatgccggt gaggaagctc
     1621 gcgaccatca cgccgaatgc gtgaatgctg ctgccgacca cgtggctcag catccgcgtc
     1681 cacagtactt cgttcaggaa agtcacggcg cctgagagca gcatcagcgg caggatccac
     1741 tgcgcggcat cgcgggttgg cggtgcggtg cgggtggccg tatcggggcg gctgcgtgcc
     1801 ggggcaaggc gtgcgatgcc ggaggcgatc acgaagacga gtccgttgat cgcggcagcg
     1861 atccagatcg ccgcgcgcag gccgaaggtc ggcaacagcc agaatgccgc gagcagcgcg
     1921 ccgcccacgg cgcccgcggt attggcggca tacagcaggc cgatgcgccg gccgatttcc
     1981 tgctccgtgt gcacggcgtg gcgcgcgagc agcggcagag tggcgcccat cagcgtcgcc
     2041 ggcacggcca gcacgacgaa ggcgctcgcc aggtaatacc aggagagcgc accatgctcg
     2101 ctcgacggcg gcgcgggctg cccgccgaac agcacatcga ggagccggct gctcgcgagc
     2161 agcagcagcg gcacgagcac gacggcgctc agcccgatgc cgagttcgag taccgcatag
     2221 gtgagtaccg ggcgcgcgat ccgcggcagc agccggtcga ccagtgccgc gccgagcgcg
     2281 aggccaccca tgtaggcggc cagtaccgtg gcgaccgcga gttccgacgt gccgaacacg
     2341 agggcgaact cgcgcgtcca ggccgtctgg taaacgaggg ctgcaagtcc actcaacgca
     2401 aagcagccac cgagcgcgag cagaacgggc agtgatcgtg ccccgccagc gtgcacgttg
     2461 acagcgtcag tggtccgcga catggtgcgc gagtctagca ctcggccgga agcgcacatg
     2521 caggccccag gcgtcattac gatcacgact gatttcggtc acaagggacc cttcgtcggc
     2581 gtcatgaagg ggcgcatcta cgggcgtctg ccgacggccc gtatcgtcga cctcacgcac
     2641 gagatcctgg tgcactggcc ggcggaagcc ggtttctggc tgacgcgcgc gtaccagtat
     2701 ttcccgaccg gtacggtgca cgttgcggtc gtcgatcccg gcgttggcac cgggcgcgac
     2761 atcatcgcgg tgagcgtcga cgggcacgtg ttcctcgcgc ccgacaacgg cctgctcgcg
     2821 cccgtcgtgg gggcgcgcga ggatgcgcgc atcgtgaaag tcgatctgca ggcgctcgcg
     2881 aggttcggag tcacgcggcc gagtgcgacc ttccacggtc gcgacatctt cgcgccgctc
     2941 gcggcggaac tcgcggccgg ccgttgccgg ccggaggatc tcggcagccc ggtcgatgtg
     3001 ctggtgccgt cctgggtgga ggaggcgagc ggcgatgaaa gcgcggtgca cggtgtcgtc
     3061 atcacgctgg accacttcgg caacctgatc accaacatcg atgcgcactg ggtcgtgcgc
     3121 atgggctcac cccgtgtcta tgccgccaac cattcctggc cgctgctgcg cacctacggg
     3181 gacgtcaagc cgggcgagta cctcgccctc gtcaattcgt tcggcgtcgt cgagatcgcc
     3241 cgggccgagc agagcgccgc ggaggggctg ggactgtccc gcggggcccc ggtggtcgtg
     3301 cgggatacgg cccgattgcc ctgaaaatca cggacttgaa agccggtgcg gattcgttat
     3361 agtgcgcgtc tcgctgtgca cgcaatgtgc gcataaatcc ttgattaact taatttttat
     3421 tgcaggcaat ggccgtcagc gaacgcgata acgagagctt cgatctggac gacgagtttc
     3481 tcggtgagac tccggccgaa gacggcacgg agtatgaccg catgctcgac aaggtggaca
     3541 agcgctcacg tgtccagccc ggcaagcgcg gcaaggcc
//
LOCUS       DAOQVY010000172         3453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_31201_length_3453_cov_5.322837, whole genome shotgun sequence.
ACCESSION   DAOQVY010000172 DAOQVY010000000
VERSION     DAOQVY010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31201_length_3453_cov_5.322837"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PKN91_11845"
     CDS             complement(<1..869)
                     /locus_tag="PKN91_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28801.1"
                     /translation="MKRLILYGSERYVIAILRPIAEAARRQGDTVAWYLGGPGAELLG
                     ADDERLADMAAVRRWRSDAVITCNDLVPYFASGVKVQVFHGFDAGKPRHIHVRGLFDL
                     YCTTGPADTAAFEALAARLGYFTVAQTGWPKLDPFFRALASQGAGAHARPRILYHSTF
                     SPSWSAAELLYDTVAALARTGRWDWTVTLHPKMNPATVAKYRALAGPNLRFADNDNIL
                     ELFPDADVMVSDTSSALHEFLLTLRPVVTCRNLRPGPYLIDIRESGELEAAIERALSP
                     PPSLLEAIRAHAQA"
     gene            complement(866..2293)
                     /locus_tag="PKN91_11850"
     CDS             complement(866..2293)
                     /locus_tag="PKN91_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HNS28802.1"
                     /translation="MTPDEFRRAGHELIDWIADYRARVAGGATPVLAQVRPGELRAAL
                     PAAPPLAAEPMEAIRRDLDALLLPACTHWQSPNFFGYFPSNSALAAVLGDLVSTGLAQ
                     LGLNWQSSPALTELEELTTDWLRQMLALPAEFRGVIQGTASDATLVALICARERATGY
                     AAAHGGLASVARPLTVYASAHSHSSVEKAALLAGFGRDHLRLVPVDEDFAMRADALAD
                     LVAEDLARGALPCAVVATVGTTGTTAIDPVGEIAAVAASHGLWLHVDGAMGGSAMILP
                     ECRALWAGIEGADSIVVNPHKWLGASMDCSVYFVRDPQHLVRVMSTNPSYLRTAADDA
                     TTNYRDWGVPLGRRMRALKLWFLIREQGVAGLQVRLRRDLTHARWFASRIDAAEHWRR
                     LAPVPLQTVCVRHEPPGVAGEALDAHTLAWVGRLNASGKAYLTPSQLEGRWMVRVSFG
                     VESTEREHVEALWRLMQRVAQGGEA"
     gene            complement(2315..2827)
                     /locus_tag="PKN91_11855"
     CDS             complement(2315..2827)
                     /locus_tag="PKN91_11855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS28803.1"
                     /translation="MDQEKIRVATPDDAAAVGELLASSYPVLMASAYEEKALAPALEL
                     MTRANPVLLSSGRFYVAATPAGLLVGCGGWSLEQPGTGTLEPGVGHLRHFATRPAWVG
                     RGLGRAIYRSCEVAALSAGVLALECYSSLNAVPFYAALGFGRVRDVAVELRPGVVLPS
                     VVMRREFRAV"
BASE COUNT          604 a         1194 c         1179 g          476 t
ORIGIN      
        1 gcctgcgcat gcgcgcggat cgcctccagc agcgatggcg gcggcgacag cgcacgctcg
       61 atcgccgcct cgagctcgcc ggattcccgg atatcgatca ggtaagggcc cggccgcagg
      121 ttgcggcagg tgaccacggg acgcagcgtg agcaggaact cgtgcagcgc cgaggacgta
      181 tccgagacca tcacatcggc gtcggggaac agctcgagga tattgtcgtt gtccgcgaac
      241 ctgaggttcg ggccggcgag cgcgcggtac ttcgcgaccg tggccggatt catcttcgga
      301 tgcagggtca ccgtccagtc ccagcggccg gtgcgcgcca gcgcggccac ggtgtcgtac
      361 agcagctccg cggcgctcca tgacggcgag aacgtggagt gatagaggat gcggggacgc
      421 gcatgcgcgc ccgcgccctg cgacgccagc gcgcgaaaga acggatcgag cttcggccag
      481 ccggtctgcg ccacggtgaa ataccccagc ctggccgcga gcgcctcgaa agcggcggtg
      541 tccgccggcc cggtggtgca gtagaggtcg aacaggccgc gcacatggat gtggcgcggc
      601 ttgccggcgt cgaacccgtg gaacacctgc accttcacgc cgctggcgaa ataaggcacg
      661 agatcgttac aggtgatcac ggcatcggag cgccagcgcc gcaccgccgc catgtccgca
      721 agccgctcgt cgtccgcgcc cagcagctcg gctcccggcc cgccgagata ccaggccacg
      781 gtatcgccct gccggcgggc cgcctcggcg atgggccgca ggatcgcgat cacatagcgt
      841 tccgaaccgt agaggatcag gcgcttcacg cctcgcctcc ctgcgcgacc cgctgcatca
      901 gccgccacag cgcctcgaca tgctcgcgct cggtactctc gacgccgaac gacacgcgta
      961 ccatccagcg cccctcgagc tgcgagggcg tgaggtaggc cttgcccgaa gcgttcaggc
     1021 ggcccaccca cgcgagcgtg tgggcatcga gcgcctcgcc cgcaacgccc ggcggttcgt
     1081 ggcgcacgca cacggtttgc agcggcaccg gcgcaaggcg ccgccagtgc tcggccgcat
     1141 cgatgcggga ggcgaaccag cgcgcatggg tcaggtcgcg ccgcagacgc acctgcaggc
     1201 ccgcgacgcc ctgctcgcgg atcaggaacc agagcttcag cgcacgcatg cgccgcccga
     1261 gcggcacgcc ccagtcgcga taattggtgg tcgcatcgtc ggcggccgtg cgcaggtaac
     1321 tgggattcgt cgacatcacg cgcacgagat gctgcgggtc gcgcacgaaa tacaccgaac
     1381 agtccatcga ggcaccgagc cacttgtgcg ggttgacgac gatcgaatcg gcgccctcga
     1441 tgcccgccca gagcgcgcgg cattcgggca ggatcattgc cgagccgccc atcgccccat
     1501 cgacgtgcag ccacaggcca tgacttgcgg ccaccgcggc gatctcgccc accggatcga
     1561 tcgcagtcgt acccgtggtg ccgaccgtcg cgaccaccgc gcacggcagc gccccgcgcg
     1621 ccagatcctc ggcgaccagg tccgcgagcg catcggcgcg catcgcgaaa tcctcgtcga
     1681 ccggcaccag ccgcaggtga tcgcgaccga agccggcgag cagcgcagcc ttctcgaccg
     1741 aactgtggct gtgcgccgag gcatagacgg tgagcggccg ggcgaccgag gcgagcccgc
     1801 cgtgcgccgc cgcgtacccg gtggcgcgct cgcgcgcgca gatgagcgcg acgagcgtcg
     1861 catcggacgc cgtaccctgg atcacgccgc gaaactcggc cggcagcgcg agcatctggc
     1921 gcagccagtc ggtggtgagc tcctccagtt cggtgagcgc cggactcgac tgccagttga
     1981 gcccgagctg cgcgagcccc gtgctcacga gatcgccgag cacggccgcg agcgcgctgt
     2041 tggagggaaa atagccgaag aaattcgggc tctgccagtg ggtgcaggcc ggcagcagca
     2101 gcgcatcgag atcgcgacgg atcgcctcca tcggttcggc cgcgagcggc ggcgcagcgg
     2161 gcagcgcggc ccgcagctcg cccgggcgca cctgcgccag cacgggcgtc gcaccgcccg
     2221 ccacgcgggc gcggtaatcg gcgatccagt cgatcaactc gtggcctgcg cggcggaatt
     2281 catccggggt catgtgtgca gtgtaaaggt gtcgtcagac ggcgcggaac tcgcgacgca
     2341 tcacgaccga gggcaagacg actcccggcc gcagctccac cgcgacatcg cgaacgcgcc
     2401 cgaagcccag cgccgcgtag aacggcaccg cattcaagct cgaatagcac tccagggcca
     2461 ggacgccggc cgacagagcc gccacttcgc acgagcgata aatggcccgc ccgaggccgc
     2521 gcccgaccca cgccggacga gtcgcgaaat gccgcagatg ccccacgcca ggctcgagcg
     2581 tcccggttcc gggctgctcg agcgaccagc cgccgcagcc gaccaacaaa cccgccggcg
     2641 tcgcggcgac gtagaacctg cccgagctca gcagaacagg gttggcccgc gtcatcagct
     2701 cgagggccgg cgcgagcgcc ttctcctcgt aggcggacgc catgagcacc ggataggacg
     2761 acgccagcag ctcgccgacg gcggcagcgt cgtccggcgt cgccaccctg attttttcct
     2821 ggtccatgcc ggcccgcgct ccagcgaagt gttggaagat gccaagcctt catgggcccc
     2881 gcaatgagct cacgcacctc gggaacccat cgggaacccg ggatggtttc gtggtcgcat
     2941 tctgcgccgt cggcaccggt agacgaagcg aggtttacca cgcaacatga acgggtagcg
     3001 tcgggggttg tcagccatat gccacgattc acccaaaaga agcatgtctg acgttttcgt
     3061 ttcttacagc catgaggacc ggccggtcgc aaagcgcttt gcggagagtc tggagcgcga
     3121 aggtttcagt gtgtggtggg accagtcgat cagcgcgggt gagaccttcg acaaggtcac
     3181 ggaaaaggcc ctgaaggacg cccggagcgt cgtggtgctg tggtcgaaac gatcggtcga
     3241 gtcgcgatgg gtgaggtctg aaggccatga tggaattgca ggtgcagatc gctcaggcgg
     3301 cggccgtcgg caccggcgtt tcggtcggcg tcggcgtggc cagaatctgc agattggccg
     3361 ggttgtacgc cgcgccgttg taggtgagcg acacgtaggt actgctgatc cagccggtga
     3421 cgccgttata ggtcacctga taccactcgc ccg
//
LOCUS       DAOQVY010000173         3445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_31318_length_3445_cov_4.621534, whole genome shotgun sequence.
ACCESSION   DAOQVY010000173 DAOQVY010000000
VERSION     DAOQVY010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31318_length_3445_cov_4.621534"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="PKN91_11860"
     CDS             <1..928
                     /locus_tag="PKN91_11860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase, exosortase A
                     system-associated"
                     /protein_id="HNS28804.1"
                     /translation="VIHAHSPALNGLAALKAGRRLGLPVVYEMRASWEDAAVDHGTSR
                     EGSLRYRASRALETRVFRRADAITTICEGLRSDIVGRGIAAGKVTVIPNAVDVAEFDA
                     AVEANDALARELGLGPGPVLGFIGSFYAYEGIDVLLRATPAILQAFPQARVLLVGGGP
                     AEESLRRLAAELGIAERVHFAGRVPHRLVSQYYALIDLLVYARHSIRLTETVTPLKPL
                     EAMAMRRMFVASNVGGHLEVVPASLRDRLFRAGDAADLARVVLEQLAKRADWPRSAEE
                     GRRYVERERSWRQSVSAYPRVYDEALKSHRAR"
     gene            925..2118
                     /locus_tag="PKN91_11865"
     CDS             925..2118
                     /locus_tag="PKN91_11865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS28805.1"
                     /translation="MKIASVCRVLPTPDNPAAGAFVLRRLAAMAGKTRLRVIQPVPHL
                     PLVRPLPAWAREPAHIAGGVTIEHAPMTYLPGLLKSLDGRWLEASVYPRLAALQAAGE
                     LDLVDAHFGYPDGVGCVRAARRLGIPAFITLRGMEVDAIRHPRIGPQLAEALRQAAGC
                     ITVSHALRDLALEAGVQPDHVLVAPNAVDRASFHPGDRAAARVRLGLEPDAPLIVSVG
                     YLIAGKGHGVLIRALARLRETLPDARLAIIGGPGYEPDHPRVLTEIAREAGVQGAVRF
                     VGAVKPDAVALWLQAADVFALATEREGCCNSVLEALAVGLPVVTTPAGDNPRFVHNGV
                     NGQLVGIGDVAGTAAALASVLDRAWDPEAISRALAVGNWSDVAEQVLGFMAARRRRSA
                     APGKP"
     gene            complement(2081..3067)
                     /locus_tag="PKN91_11870"
     CDS             complement(2081..3067)
                     /locus_tag="PKN91_11870"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNS28806.1"
                     /translation="MNAISGIESGLVRGAGSLLSRRGASASLLVLIYHRVLPAPDPML
                     PSEPDAAAFAGLMDLLAQNFTVLPLREAVARLRQGSLPPRAVCITFDDGYANNFTVAM
                     PLLRARGLPATVFVAPAYLNGGAMFNDLVFEACRVAPADFDLTELGLGRVALTDAGAR
                     IQAAQQVVDRLKYLTPTERRQAAERLVERVNATGSVDLMMTDEQVAEMSRQGIEIGAH
                     TMTHPILARIPESTARDEIVQSRQRLEAITGQPVLSFAYPNGRPVRDYASEHVRAVRD
                     AGFELAVSTSWGAATAQSDPFQIPRIAPWDKSPLRYAARMVKAYRERHFACA"
     gene            3264..>3445
                     /locus_tag="PKN91_11875"
     CDS             3264..>3445
                     /locus_tag="PKN91_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNS28807.1"
                     /translation="MKRRDFLVTTAGMAGAAIPGIGFGQIKPCPPPGVGMGGGTSAST
                     PCATPTAAADWQARISG"
BASE COUNT          506 a         1165 c         1218 g          556 t
ORIGIN      
        1 cgtgatccat gcccattccc cggcgctgaa cgggcttgcg gcgctcaagg cgggcaggcg
       61 actcggcctg cccgtggtct acgagatgcg cgcctcctgg gaagacgcgg ccgtggacca
      121 tggcacctcc cgcgagggga gcctccgcta ccgcgcctcg cgcgcgctcg agacgcgcgt
      181 gttccgccgc gcggatgcga tcacgacgat ctgcgaggga ctgcgcagtg acatcgtcgg
      241 gcgcgggatc gcggccggca aggtcaccgt gataccgaat gccgtcgatg tggccgagtt
      301 cgacgctgct gtcgaggcga acgatgcact cgcgcgcgaa ctgggtctcg ggcccggccc
      361 ggtgctcggc ttcatcggct ccttctacgc ctacgagggc atcgatgtcc tgctgcgcgc
      421 gactccggca atccttcagg ccttcccgca ggcacgcgtc ctgctcgtgg gcggcggccc
      481 cgcggaggaa tcgttgcggc gcctcgccgc ggagctcggc atcgccgagc gggtgcactt
      541 cgccggccgc gtgccgcacc gcctcgtcag ccagtactac gcgctgatcg acctgctggt
      601 ctatgcccgc cattcgatcc ggctcaccga gacggtcacg ccgctcaagc cgctcgaggc
      661 gatggcgatg cgccgcatgt tcgtcgcgtc caatgtgggc ggtcacctgg aagtggtgcc
      721 cgcctcactg cgcgaccggc tgttccgcgc gggcgatgct gccgacctcg cgcgcgtcgt
      781 actcgagcag ctggcgaagc gcgccgactg gccgcgcagc gcggaggagg ggcggcgcta
      841 cgtcgagcgg gagcgaagct ggcgacagtc cgtcagtgcc tacccgcggg tctatgacga
      901 ggcgttgaag agccaccgcg cgcgatgaag atcgcgagtg tctgccgggt cctgccgacg
      961 ccggataatc cggcggctgg tgcgttcgta ctgcgacgac tcgcggcgat ggcgggcaag
     1021 acgcggctgc gcgtgatcca gcccgtgccg cacctgccgc tcgtgcgccc gctgcccgcc
     1081 tgggcgcgcg agcctgcgca tatcgccggc ggcgttacga tcgaacacgc gcccatgacc
     1141 tacctgccgg gcctgctcaa gtcgctggac gggcgctggc tcgaggcgag cgtctatcca
     1201 cgtctcgcgg ccctgcaggc cgcgggcgag ctggatctgg tcgatgccca cttcggctat
     1261 cccgacggcg tcggctgcgt gcgcgctgcg aggcgcctcg gcattcccgc gttcatcacg
     1321 ctgcgcggca tggaggtcga cgccatccgc catccccgga tcggtccgca gctcgccgag
     1381 gccctgcgcc aggcagccgg ctgcatcacc gtgagccatg cgctgcgcga tctcgcactc
     1441 gaggcaggcg tgcaaccgga tcatgtgctc gtggcgccca acgcggtcga tcgcgcgtcg
     1501 ttccacccgg gtgatcgcgc cgcggcgcga gtccggctgg ggctggaacc ggatgcgccg
     1561 ctgatcgtca gcgtggggta cctgattgcc ggcaaggggc acggggtcct gatccgggca
     1621 ttggcgaggt tgcgcgaaac gcttcccgac gcacgcctcg ccatcatcgg cgggcctggc
     1681 tacgaacccg atcatccgcg cgtgctcacc gaaatcgcgc gcgaggccgg cgtgcaggga
     1741 gcggtgcggt tcgtcggcgc cgtcaagccc gacgccgtcg ccctatggtt gcaggcggcc
     1801 gatgtgtttg cgctcgccac cgaacgcgag ggctgctgca attcggtgct cgaggcactg
     1861 gcagtcggcc tgccggtcgt gacgacgcct gccggagaca acccgcgctt cgttcacaac
     1921 ggcgtcaacg gccaactggt cggcatcggt gatgtggccg ggaccgcggc cgcgctcgcc
     1981 tcggtgctcg accgggcctg ggatcccgag gccatctcgc gagcgctggc tgtcggcaac
     2041 tggagcgacg tggccgagca ggtgctcggc ttcatggccg ctaggcgcag gcgaagtgcc
     2101 gctcccggta agccttgacc atgcgtgcgg cgtagcgcag cgggctcttg tcccacggcg
     2161 cgatgcgcgg gatctggaag gggtcggact gcgccgtggc cgcgccccaa ctcgtcgaca
     2221 ccgcgagctc gaatccggca tcgcgcacgg cgcgcacgtg ctcgctggcg taatcgcgca
     2281 ccggcctgcc gttcgggtac gcaaagctga gtaccggctg tccggtaatc gcttcgagcc
     2341 gctgcctgct ctgcacgatt tcgtcgcgtg cggtcgattc cggaatccgt gcgaggatcg
     2401 ggtgcgtcat cgtgtgcgcg ccgatctcga tgccctggcg ggacatttcc gcaacctgct
     2461 cgtcggtcat catgagatcg acgctgccgg tggcattcac gcgctccacc aatcgctcgg
     2521 cggcctgtcg acgctcggtg ggagtgaggt acttgagccg atccaccacc tgctgcgcgg
     2581 cctggatgcg cgcgcccgca tccgtgagag ccacgcgacc gagtcctaat tcggtcaggt
     2641 cgaagtcggc gggcgcgacg cggcaggcct cgaaaacgag gtcgttgaac atggcgccgc
     2701 cgttcaggta ggccggtgcg acgaacaccg tggccggcaa cccgcgtgcg cgcagcagcg
     2761 gcatcgcgac cgtgaaattg ttcgcatagc cgtcgtcgaa agtgatgcag acggcgcgcg
     2821 ggggcaggct accctggcgc aggcgcgcga ccgcttcacg cagcggcagc accgtgaagt
     2881 tctgcgcgag caggtccatg aggcccgcga acgcggccgc atccggctcg ctcggcagca
     2941 tcggatcggg cgcaggcagg acccggtgat agatcagcac gaggagcgaa gcactcgcac
     3001 cacggcggga aagcagggaa cccgcgccgc ggacaaggcc agattcgatg ccggaaatcg
     3061 cgttcatgga cgggtcatac cagagcggat caacaatgcg ggcatgatat ccgcgttctg
     3121 agagtgtttg cttctggcga catggtgcaa tttgtggact ggttcactaa cttaggcgcc
     3181 tgttcgcatt tgatggatag cccgggaggg gggacacttc ctgccgtggc acagagcgcg
     3241 cgcactcgcg cgggagcaac ttgatgaagc gcagagattt cctggtgacc accgccggca
     3301 tggcgggcgc cgcgattccc ggtattggat ttggccagat caagccctgc ccgcctccgg
     3361 gtgtaggcat gggcggtggc acttccgcct cgacgccgtg cgcaacgccg accgccgcgg
     3421 cggattggca ggcgcgtatc agtgg
//
LOCUS       DAOQVY010000174         3276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_33602_length_3276_cov_5.186588, whole genome shotgun sequence.
ACCESSION   DAOQVY010000174 DAOQVY010000000
VERSION     DAOQVY010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33602_length_3276_cov_5.186588"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..270
                     /locus_tag="PKN91_11880"
     CDS             94..270
                     /locus_tag="PKN91_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28808.1"
                     /translation="MADDERKLAEFRTKAHDSLALDHAGALVYLAPTRVNLALTQERW
                     PDIRFLETREHLAA"
     gene            296..1564
                     /locus_tag="PKN91_11885"
     CDS             296..1564
                     /locus_tag="PKN91_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS28809.1"
                     /translation="MAKVLTRKPVVSWALYDWANSAFATTVMAGFFPLFFKQYWNAGV
                     EATESTFRLGVTSGFTSLIVALLAPIVGAIADKSGARVKLLALFTVLGAAMTAALALV
                     GQGEWFAAALLYGAASIGFWGGNQFYDSLLTDVAEEPAYDLVSGYGFSLGYLGGGVLF
                     AVNAVMVSKPHLFGIADAGTAVKLSFLSVAVWWLVFSLPVVLFVRERPGAAPLPMRAA
                     VSAGWRELRHTVAHVRESRAMLWFLLAYWFYIDGVNTIIKMAVDYGLALGFRQASLIQ
                     ALLLVQFVGFPAALAFGWLGARIGARTGILIALAVYTGVTLYAYRLSSEAEFFLLAVV
                     IGCVQGGVQSLSRSFYGRLVPPGQSGEFFGFYNMMGKAAAILGPLLAGTVALVSGDSR
                     LAMLSIALLFIIGGALLLKVPQGQPAGSTP"
     gene            complement(1534..2340)
                     /locus_tag="PKN91_11890"
     CDS             complement(1534..2340)
                     /locus_tag="PKN91_11890"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HNS28810.1"
                     /translation="MTVPWILAVLVAAGLAWTLVRAQRRRRMLHMPFPAAWRALLAER
                     VSLYRRLPPGMRPRVELAARRLLARTDFIGCGGLEVTEDMRLIIASQAAVLVANRDPH
                     AYDALYSVLVYPSEFVVEDRIEDEYGLVTEGSRALSGQTIDVARIVLSWDDVEAGLAG
                     DDGYNVVLHEFAHHIDHVMEGALTDGGALAPAAARWHATLQSEYDALCDAVDRDEDTL
                     IDPYGAEDLVEFFATATEVFFERAVEFRAAHPRLHAQLCDFYGVDPAGWP"
     gene            complement(2337..>3276)
                     /locus_tag="PKN91_11895"
     CDS             complement(2337..>3276)
                     /locus_tag="PKN91_11895"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNS28811.1"
                     /translation="QNPRTLALAARLRAAAPDATRYARAVLDYFAREGFEYTLTPPRL
                     AANAVDDFLFNTRRGFCGHYASAYATLMRAGGVPARVVTGYLGGEWNPIGGYLLVRQS
                     DAHAWVEIWLEGRGWARVDPTGVVAPERLQRGLYDLMPAAASFTTRLTRDTAWLGELV
                     YAWDAVNTAWRKRVLEFGQQSQLKLLDRLGFDSPHWRHLGYALVAALLAWLAWMSWQS
                     RMWLRAARPDSLARAWRALGAKYARIGLARAPAEAPLAHAARVGAARPDLAATADALA
                     RRYADLRYGPAGSADEVRRFAHDVRRLRLPAATRSA"
BASE COUNT          460 a         1104 c         1183 g          529 t
ORIGIN      
        1 acgatcttga tcgggatgcg ggcggtcttg tcggcgccgc gctgcttgcg ctcgtcgtcc
       61 gccacgatcc agcgggccgt gtgcacgttg atcgtggcgg acgacgagcg caagctcgcg
      121 gagtttcgca ccaaggccca cgacagcctc gcgctcgacc acgccggcgc gctggtctac
      181 ctggcgccga cccgggtgaa tctcgcgctc acccaggagc gctggcccga tatccggttc
      241 ctcgagacgc gcgaacacct cgccgcctga tcggcgcggg tgatagcctg tcgcgatggc
      301 gaaggtcctc acccgcaagc ccgtggtgtc ctgggcgctc tacgactggg cgaactcggc
      361 cttcgcaacc acggtcatgg cggggttctt tccgcttttc ttcaaacagt actggaacgc
      421 cggggtcgag gcgaccgaga gcacgttccg tctcggcgtc acgagcgggt tcacgagcct
      481 gatcgtcgcg ctgcttgcgc cgatcgtcgg cgcgattgcc gacaagagcg gcgcgcgggt
      541 caagctgctg gcgctgttca ccgtgctcgg cgccgcgatg acggcggcgc tggcgctggt
      601 cgggcagggt gagtggttcg cagccgcgct gttgtatggc gcggcctcga tcggtttctg
      661 gggcggcaac cagttctacg actcgctgct gaccgacgtc gccgaggagc ccgcgtacga
      721 tctcgtgtcg ggttacggct tctcgctcgg ctatctcggc ggcggcgtgc tgttcgcggt
      781 gaacgccgtg atggtgtcga agccgcactt gttcggtatc gccgacgccg gcacggccgt
      841 gaagctgtcg ttcctcagtg tcgcggtgtg gtggctggtg ttctcgctgc cggtggtgct
      901 gttcgtgcgc gagcggcccg gcgcggcgcc gctgccgatg cgcgcggcgg tgtcggccgg
      961 ctggcgcgag ctgcgccaca ctgtcgcgca cgtgcgcgag agccgcgcga tgctctggtt
     1021 cctgctcgcg tactggttct acatcgacgg cgtcaacacg atcatcaaga tggccgtcga
     1081 ctacggcctc gcgctcggct tccggcaggc gagcctgatc caggcactgc tgctcgtgca
     1141 gttcgtcggc tttccggccg cgctcgcctt cggctggctc ggtgcgcgca tcggcgcgcg
     1201 caccggcatc ctgatcgcgc tcgccgtgta caccggcgtc acgctgtacg cctaccgcct
     1261 gagttccgag gcggagttct tcttgctcgc ggtcgtgatc ggctgcgtgc agggcggcgt
     1321 gcagtcgttg tcgcggtcgt tctacggccg gctcgtgccg ccgggccagt cgggcgagtt
     1381 cttcggcttc tacaacatga tgggcaaggc cgcggcgatc ctcggaccgc tgctcgccgg
     1441 caccgtggcg ctcgtaagcg gggactcacg gctcgcgatg ctctcgatcg cgctgctgtt
     1501 catcatcggc ggggcgctgc tgctgaaagt tcctcagggc cagccggccg ggtccacgcc
     1561 gtagaagtcg caaagttgcg cgtgcaggcg cggatgcgcg gcacggaact cgacggcacg
     1621 ctcgaagaac acttcggtcg ccgtggcaaa gaactcgacc aggtcctcgg cgccatacgg
     1681 gtcgatcagg gtgtcctcgt cgcgatcgac ggcatcgcac agcgcgtcgt attcggactg
     1741 cagcgtcgca tgccagcgcg cggcggccgg cgccagtgcg ccgccatcgg tgagtgcgcc
     1801 ctccatgaca tggtcgatat ggtgcgcgaa ctcgtgcagc acgacgttgt agccgtcgtc
     1861 gcccgcgagc cccgcctcga cgtcgtccca ggaaagcacg atgcgcgcca cgtcgatggt
     1921 ctggcccgac agcgcacggc tcccttcggt gacgaggccg tattcatcct cgatgcgatc
     1981 ctcgaccacg aactccgacg gatagaccag cacgctgtag agcgcgtcgt aggcgtgcgg
     2041 atcccggttc gccaccagta ccgcggcctg gctggcgatg atgagccgca tgtcttcggt
     2101 gacttcgagc ccgccgcagc cgatgaagtc cgtgcgcgcg agcaggcggc gcgcggcgag
     2161 ttccactcgc ggacgcattc cgggcggcag gcgccgatag agtgacacgc gctcggcgag
     2221 cagggcacgc cacgcggcag ggaagggcat gtgcagcatg cgccggcgcc gctgcgcgcg
     2281 cacgagtgtc cacgcgaggc ccgcggccac cagcacggcc aggatccacg gcacagtcat
     2341 gcgctgcgtg tcgccgcggg caggcgcagg cggcgcacgt cgtgtgcgaa gcggcgcact
     2401 tcgtccgcac tgcccgcagg accgtagcgc aggtcggcgt agcggcgcgc gagggcatcc
     2461 gcagtggcgg cgaggtcggg gcgcgcggcg cctacgcgcg cggcatgcgc gagcggtgct
     2521 tccgcaggtg cgcgcgcgag gccgatgcgc gcgtacttcg cgccgagcgc gcgccacgcc
     2581 cgcgcgaggc tgtcgggccg cgcggcgcgg agccacatgc gcgattgcca cgacatccag
     2641 gcgagccagg cgaggagcgc ggcaacgagc gcatagccca ggtggcgcca gtgtggcgag
     2701 tcgaagccga ggcgatcgag cagcttgagc tgcgactgtt ggccgaactc gagtacgcgc
     2761 ttgcgccagg ccgtgttgac cgcatcccac gcgtacacga gctcgccgag ccaggccgtg
     2821 tcgcgcgtga gacgggtcgt gaacgaggct gcggcgggca tcaggtcgta gagtccgcgc
     2881 tgcaggcgct ccggcgcgac gacgccggtc ggatcgacac gggcccagcc gcgcccctcg
     2941 agccagatct ccacccaggc gtgcgcgtcg gactgcctga ccagcagata gccgccgatc
     3001 ggattccact cgccgccgag ataaccggtg acgacccggg caggaacgcc gccggcacgc
     3061 atcagcgtgg cgtaggccga ggcatagtgg ccacagaagc cacggcgcgt attgaacagg
     3121 aaatcgtcga ccgcattggc ggcgagcctg ggtggcgtca gcgtgtactc gaatccctcg
     3181 cgcgcgaaat aatccagcac ggcgcgcgcg tagcgggtcg cgtccggcgc ggcggcgcgc
     3241 agtctggcgg cgagtgcgag cgtgcgcggg ttctgg
//
LOCUS       DAOQVY010000175         3269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_33714_length_3269_cov_5.400436, whole genome shotgun sequence.
ACCESSION   DAOQVY010000175 DAOQVY010000000
VERSION     DAOQVY010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33714_length_3269_cov_5.400436"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /gene="hflK"
                     /locus_tag="PKN91_11900"
     CDS             <1..775
                     /gene="hflK"
                     /locus_tag="PKN91_11900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNS28812.1"
                     /translation="AVNTVKYESRVLTADVNLVNLALAVQYQFRDPEKVLFGVRDPES
                     SLREVSESAIREVVGRGDLEAILVSARQQVTERTKELIQTTLDYYGTGIRVTSVNLTE
                     VQVPEAVVPSQRDANKAIADRDRLQKEAEAYQSGIIPVAEGAASRQIQDAEAYKAQVT
                     AVAEGEAARFSQLRAAYEAAPAVTRKRMYLDTVEGVLKSSKKVVIDSKGVGNNLIYLP
                     LDKLTEGRAAGGNTGMRPAAAANTESETVTVEGRQRGER"
     gene            775..1650
                     /gene="hflC"
                     /locus_tag="PKN91_11905"
     CDS             775..1650
                     /gene="hflC"
                     /locus_tag="PKN91_11905"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNS28813.1"
                     /translation="MSSRITPIILVVVALLFVATSSLFTVQQTQLAIRTQFGEIQGSD
                     YGPGLHMKWPFIDQVARFERRVVTQNYVGETFLTNENRGLLVDFYVKWRVADAAKYFR
                     ATGGNEDAAGQRLADIVKDGIKNAVAQRTLQEVVTAERAAVTGEMFGRASEAVNEFGI
                     ELMDVRVQRIDLPDDVAARVYDNMKQNFEKQARQLRGEGEKDNQRIKAEADRKRTEIL
                     SAAARDALRIRGEGDAIAAATYAKAYERNPEFYAFYRSMQAYKTAIGKEGDVIVVSPD
                     SEFFKYFKESAPGNR"
     gene            1654..1854
                     /locus_tag="PKN91_11910"
     CDS             1654..1854
                     /locus_tag="PKN91_11910"
                     /inference="COORDINATES: protein motif:HMM:NF021366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HNS28814.1"
                     /translation="MIVLEWSDLAGALALLLVIEGLLPFANPAGMKRTMARVAALGER
                     ELRIAGLGSMLVGLVLLYFVRA"
     gene            1858..3117
                     /locus_tag="PKN91_11915"
     CDS             1858..3117
                     /locus_tag="PKN91_11915"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNS28815.1"
                     /translation="MGRFVVVIGTQWGDEGKGKIVDLLTERAAAVARFQGGHNAGHTL
                     VIGGKKTILSLLPSGILREGVQCLIGNGVVLSLEALFREAQTLIGEGVPVFERLRVSP
                     GCPLILPSHVALDRAREQARGANAIGTTGRGIGPAYEDKVARRAVRIADLFQRKLFAA
                     KLGELLDFHNFVLHKYFRQPAIDFQQTLDEQLAYAERLAPLMTDVGAALHALRVAGAD
                     VMFEGAQGALLDVDHGTYPYVTSSNTTAGYAATGTGIGPRTLDYVLGIVKAYTTRVGA
                     GPFPTELFDEAGEHLSRVGHEVGSVTGRARRCGWFDAVALRRSIVLSSVSGLCITKLD
                     VLDGLDTLQICVEYRPGAQPGEVTPVYEELPGWRESTIGITSYEALPGNARRYLERIA
                     ELTATPLDVVSTGPDREQTIVLRHPFG"
BASE COUNT          556 a         1096 c         1112 g          505 t
ORIGIN      
        1 ggcggtcaac accgtcaagt acgaatcacg cgtgctcact gccgacgtga acctggtcaa
       61 cctggccctc gccgtgcaat accagttccg cgatccggag aaggtgctgt tcggggtgcg
      121 cgacccggaa tcctcgctgc gtgaagtcag cgagagcgcg attcgcgaag tcgtcggtcg
      181 cggcgacctc gaggcgatcc tcgtctcggc ccgccagcag gtcaccgagc gcaccaagga
      241 gctgatccag accaccctcg actactacgg caccggtatc cgcgtcacca gtgtcaacct
      301 gaccgaagtg caggtgcccg aggccgtcgt accgtcgcag cgcgatgcca acaaggcgat
      361 cgccgaccgc gaccgcctgc agaaagaggc cgaggcgtat cagagcggca tcattccggt
      421 cgcggaaggt gccgcttccc gccagatcca ggatgccgag gcatacaagg cgcaggtgac
      481 ggcggtggcc gagggtgagg cggcgcgctt ctcgcagctg cgtgccgcgt acgaggctgc
      541 cccggcggtc acccgcaagc gcatgtacct cgataccgtc gagggcgtat tgaagagctc
      601 gaagaaagtc gtgatcgatt cgaagggcgt cggcaataac ctgatctacc tgccgctcga
      661 caagctgacc gaaggtcgcg ctgcgggcgg caacaccggc atgcggcccg ccgcggccgc
      721 gaacaccgag tccgagaccg tcacggtcga gggccgccag cggggagagc gctgatgtcc
      781 tcgcgtatca cgcccatcat cctcgtcgtc gtcgcgctgc tgttcgtcgc gacctcctcg
      841 ctgttcacgg tgcagcagac ccaactcgcg atccggaccc agttcggtga gatccagggc
      901 tccgactatg gcccgggtct gcacatgaag tggccgttca tcgaccaggt cgcgcgcttc
      961 gagcggcggg tggtcaccca gaactacgtc ggcgagacct ttctcaccaa cgagaaccgc
     1021 ggcctgctgg tcgacttcta cgtcaagtgg cgcgtcgccg acgcggccaa gtacttccgc
     1081 gccaccggcg gcaacgaaga cgcggccggc cagcgcctcg ccgacatcgt caaggacggc
     1141 atcaagaacg ccgtcgcgca gcgcacgctg caggaagtcg tgaccgccga gcgcgcggcc
     1201 gtgaccggcg agatgttcgg ccgtgcgagc gaggcggtca acgagtttgg catcgaactg
     1261 atggacgtgc gcgtgcagcg catcgacctg ccggacgatg tcgcggcgcg cgtctacgac
     1321 aacatgaagc agaacttcga gaagcaggcg cgccagttgc gcggcgaggg cgagaaggac
     1381 aaccagcgca tcaaggccga agccgaccgc aagcgcaccg aaattctctc ggccgccgcg
     1441 cgcgacgcgc tgcgcatccg cggcgagggc gatgcaatcg ccgcggccac ctacgcgaag
     1501 gcctacgagc gcaatcccga gttctatgcc ttctaccgca gcatgcaggc gtacaagacg
     1561 gcgatcggca aggagggcga cgttatcgtc gtcagccccg acagcgagtt cttcaagtac
     1621 ttcaaggaat ccgcgccggg taaccggtag gccgtgatag tgctcgagtg gtccgacctg
     1681 gccggcgcgc tggcgctgct gctcgtcatc gaggggctgc tgccgttcgc caatcccgcc
     1741 ggcatgaagc gcacgatggc gcgcgtcgct gcgctcggcg agcgggaact gcgcattgcg
     1801 ggtcttgggt cgatgctggt cggcctcgtg ctgctttact tcgtgcgggc gtaaaccatg
     1861 ggcagattcg tcgtcgtcat cggcacccag tggggtgatg aaggcaaggg caagatcgtc
     1921 gatctgctga ccgagcgcgc cgccgcggtt gcgcgtttcc agggcggaca caacgccggg
     1981 cacacgctcg tgatcggcgg caagaagacg attctgtcac tgctgccctc gggcatcctg
     2041 cgcgagggcg tgcagtgcct gatcggcaac ggcgtagtgc tgtctctcga ggcgctgttc
     2101 cgcgaggcgc agacgctgat cggcgaggga gtgccggtgt tcgagcggct gcgcgtctcg
     2161 ccgggttgcc cactgatcct gccctcgcac gtcgcgctcg atcgtgcgcg cgaacaggcg
     2221 cgcggcgcca acgcgatcgg cacgaccggc cgcggcatcg ggccggccta cgaggacaag
     2281 gtggcgcggc gcgcggtgcg catcgccgat ctgttccagc gcaagctgtt cgccgcgaag
     2341 ctcggcgagc tgctcgactt ccacaatttc gtgctgcaca agtattttcg ccagccggcg
     2401 atcgacttcc agcagaccct cgacgaacag ctggcgtacg ccgagcgtct cgcgccgctc
     2461 atgaccgacg tgggcgcggc gctgcatgcg ctgcgcgtgg ccggggcgga cgtgatgttc
     2521 gagggggcgc agggcgcgct gctcgacgtc gaccacggta cctaccccta cgtcacctcg
     2581 tccaacacca cggccggcta cgcggccacc ggtaccggaa tcggcccgcg cacgctcgac
     2641 tacgtgctcg gcatcgtcaa ggcatacacg acgcgggtcg gtgccggccc gttcccgacc
     2701 gagctgttcg acgaggcggg cgagcatctc tcgcgtgtcg gtcacgaggt cggctcggtc
     2761 accgggcggg cgcggcgttg cggctggttc gatgccgtcg ccctgcgccg ctcgatcgtg
     2821 ctctcgagcg tgtcgggcct gtgcatcacc aagctcgacg tgctcgacgg gctcgatacg
     2881 ctgcagatct gcgtcgagta ccgcccgggg gcgcagccgg gcgaggtgac gccggtctac
     2941 gaagagctgc cgggctggcg ggaatccacg atcggtatca cctcctatga ggcgctgccc
     3001 ggcaatgcgc gccgctacct cgagcgcatc gccgagctca ccgccacgcc gctcgatgtc
     3061 gtttcgaccg ggcccgatcg agaacagacg atcgtgctgc gccacccgtt cggctgagcg
     3121 gcggcgcacc gccgctcaca gcggcagcag ccaggggatg agcgtcacgc tgaccacgag
     3181 cgcgatcacg ccgtagggcg cgccgacctt caggaagtcg atgaagcggt aattgccggg
     3241 gcccacgacc agcgtgttca ccggtgagg
//
LOCUS       DAOQVY010000176         3234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_34304_length_3234_cov_5.797735, whole genome shotgun sequence.
ACCESSION   DAOQVY010000176 DAOQVY010000000
VERSION     DAOQVY010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34304_length_3234_cov_5.797735"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PKN91_11920"
     CDS             complement(<1..398)
                     /locus_tag="PKN91_11920"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HNS28816.1"
                     /translation="MQSRVVAGLGEIAPPDWDALVPDGTPFLRHAFLRGLESTGCVGA
                     DTGWASTHVLLTDARGLAAAAPAYLKDHSYGEFVFDFAWAQAYARSGLEYYPKLVVAV
                     PFTPATGPRLLVRADLDYGATARRLIGRLEA"
     gene            complement(407..1372)
                     /gene="trxB"
                     /locus_tag="PKN91_11925"
     CDS             complement(407..1372)
                     /gene="trxB"
                     /locus_tag="PKN91_11925"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNS28817.1"
                     /translation="MTTPKHRRLIILGSGPAGYSAAVYAARANRAPMLITGVEQGGQL
                     MTTTDVDNWPGDVEGLQGPELMERMRKHAERFSTEIVGDHIHTVDLAERPFVLHGDSG
                     RYTCDALIIATGASARYLGLPSEEEFRGRGVSACATCDGFFFRGQRVAVIGGGNTAVE
                     EALYLANLAEHVTLVHRRDRLRAEKILQDRLFERERAGKVEIVWNHAVEAVLGDAQGV
                     TGLQLASTTDGSQRRLDVTGAFIAIGHTPNTSLFTGQLDMHDGYIRVQGGAEGNATAT
                     SVPGVFAAGDVADRVYRQAITSAGSGCMAALDADKYLERHDAQGD"
     gene            1549..>3234
                     /locus_tag="PKN91_11930"
     CDS             1549..>3234
                     /locus_tag="PKN91_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNS28818.1"
                     /translation="MRESAVIIVAALAIVLLTALASYSPTDPGFSFTGSSEVVQNRIG
                     PLGAWLADILFFLFGRPAFLFPLMLGAASVAIHRGARGEESASRANSAVRVCGFLLVL
                     LTSCALATLHWAPGSLPQSAGGVVGSLVGGGLDAGLGLLGATLALLAAFMAGVSLAFG
                     VSWFTIMDRIGAATWNAIGWVRARASAARDAAEGRERRLARKEVTVADQKKAATRVKP
                     RIEQPAPRVEKSERVEKERQVPLFESGQAGELPALKLLDDPPVQEQSYSPEALEAMSR
                     LVELKLKDFGVEVEVVAVQPGPVVTRFELRPAPGVKVSQISSLAKDLARALSAISVRI
                     VEVIPGKPVMGLEIPNEKRELVTLGEIIKSRAYDELASPLALALGKDIGGQPVVADLA
                     RMPHLLIAGTTGSGKSVAINAMVLSLLYKSTAEHVRLVMIDPKMLELSVYEGIPHLLA
                     PVVTDMKQAANALRWSVAEMERRYQLMAALGVRNIAGFNKRVREANDAGKPIRDPVMT
                     AKAANDPTIDQSQIPDLMPLPYIVVIIDELADLMLIVGKKVEELITRLAQKARA"
BASE COUNT          505 a         1079 c         1108 g          542 t
ORIGIN      
        1 gcttcgagcc gcccgatcag ccgccgcgcg gtggcgccgt aatcgagatc ggcgcgcacc
       61 agcaaacgcg gcccggtagc cggggtgaag ggaaccgcga ctaccagctt cgggtagtat
      121 tcgagcccgc tgcgggcata ggcctgcgcc caggcgaaat cgaacacgaa ctcgccgtag
      181 gaatgatcct tgaggtaggc gggcgccgcg gcggccagcc cgcgcgcatc ggtgagcagt
      241 acgtgcgtgc ttgcccagcc cgtgtccgcg ccgacgcaac ccgtgctttc gagtccccgg
      301 agaaatgcgt ggcgcaggaa cggcgtgccg tccggtacca gcgcgtccca gtcaggcggc
      361 gcgatttccc cgaggccggc gacgactcgg ctctgcattg tcccgttcag tcgccctgtg
      421 cgtcgtggcg ctcgaggtat ttgtcggcat cgagcgcggc catgcagccc gagccggccg
      481 aggtgatggc ctgccgatag acgcgatcgg cgacatcgcc cgcggcaaac acgccaggca
      541 cgctggttgc cgtggcgttg ccctccgcgc cgccctgcac ccggatgtaa ccatcgtgca
      601 tgtcgagctg gcccgtgaac agcgacgtat tcggcgtatg gccgatagcg atgaaggccc
      661 ccgtgacatc gagcctgcgc tggctgccat ccgtggtcga ggcaagctgc aggccggtga
      721 cgccctgcgc atcgccgagc acggcctcga cggcgtggtt ccagacgatt tcgaccttgc
      781 cggcacgctc gcgctcgaac aggcggtcct ggaggatctt ctcggcgcgc aacctgtcgc
      841 gacgatgcac cagcgtcacg tgctccgcga gattggcgag atacagggct tcctcgaccg
      901 cggtgttgcc gccgccgatc accgccacac gctgtccgcg gaagaagaag ccgtcgcagg
      961 ttgcacaggc cgatacgccg cgaccgcgga attcctcctc cgaaggcagg ccgaggtaac
     1021 gcgccgaggc gcccgtggcg atgatcagcg catcgcaggt gtatcgaccc gaatcgccgt
     1081 ggagcacgaa cggccgttca gcgaggtcga cggtgtggat gtgatcgccc acgatttcag
     1141 tcgagaagcg ctcggcgtgc ttgcgcatgc gctccatcag ttccgggccc tgcagtccct
     1201 cgacatcgcc cggccagttg tcgacgtcgg tcgtcgtcat cagctgtccg ccttgctcca
     1261 cgccggtgat gagcatgggc gcgcggttgg cccgcgcggc atagaccgct gcgctgtaac
     1321 cggcaggtcc ggaaccgagg atgatcaggc ggcgatgctt cggtgtcgtc atgtataatt
     1381 tgcgggtcgt aaagtgggca gcggagtgta cagtatccgc attgcagccc gaatatcttc
     1441 catttaaaat caaaagattg tcgatgatca ttcatcagga ttcttgattt ggccgacgcc
     1501 cgttcctccc gcccgcccgc ccgtttctcc gccgccgtct cgcgtggcat gcgcgaaagc
     1561 gcggtgatca tcgtcgcggc actcgcgatc gtgctgctga ccgcgctcgc gagttacagc
     1621 cccaccgatc ccggtttttc cttcaccggc agttccgagg tcgtacagaa ccgcatcggc
     1681 ccgctcggcg cctggctcgc cgacatcctg ttcttcctgt tcggtcggcc ggcattcctt
     1741 tttccgttga tgctcggcgc cgcgagtgtc gccatccacc gcggtgcgcg cggcgaggaa
     1801 tcggcctcgc gcgccaactc ggccgtgcgc gtgtgcggat tcctgctggt gctgctgacg
     1861 agctgcgcgc tcgcgacgct gcattgggcg ccagggtcgc tgccgcagtc ggcgggcggc
     1921 gtggtcggca gcctggtcgg cggcggtctc gatgccggtc tcggcctgct cggcgccacg
     1981 ctcgcgctgc tggccgcgtt catggccggc gtgtcgctcg ccttcggcgt gtcctggttc
     2041 acgatcatgg accgtatcgg cgcggctacc tggaacgcga tcggctgggt gcgggcgcgc
     2101 gcgtccgccg cacgcgatgc agccgagggc cgcgagcgcc ggctcgcgcg caaggaagtg
     2161 accgtggccg accagaagaa ggctgcgacg cgcgtcaagc cgcgcatcga gcagcctgcg
     2221 ccgcgtgtcg agaagagcga acgcgtcgag aaggagcggc aggtgccgct gttcgagtcg
     2281 ggccaggcgg gcgagctgcc ggcgctgaag ctgctcgacg atccgccggt ccaggagcag
     2341 tcgtactcgc ccgaggcgct cgaggcgatg tcgcgcctcg tcgagctcaa gctgaaggat
     2401 ttcggcgtcg aggtcgaggt cgtggccgtg cagccggggc cggtcgtcac gcgcttcgaa
     2461 ctgcggccgg cgccgggcgt caaggtgagc cagatttcga gcctcgcgaa ggacctcgcg
     2521 cgcgcactgt cggcgatcag cgtgcgtatc gtcgaggtga tcccgggcaa gccggtcatg
     2581 ggcctcgaga tcccgaacga gaagcgcgag ctggtcacgc tcggcgagat catcaagtcc
     2641 agggcttatg acgaactcgc ctctccgctc gcgctggcgc tcggcaagga tatcggcggg
     2701 cagcccgtgg tcgccgacct cgcgcgcatg ccgcacctgc tgatcgccgg cacgaccggc
     2761 tcgggcaagt cggtggcgat caacgcgatg gtgctgtcgc tgctgtacaa gtccacggcc
     2821 gagcacgtgc ggctcgtgat gatcgacccg aagatgctcg agctgtcggt ctacgagggc
     2881 atcccgcacc tgctcgcacc ggtcgtcacc gacatgaagc aggccgcgaa cgcgctgcgc
     2941 tggtcggtgg cggagatgga gcggcgctac cagctgatgg cggcgctcgg cgtgcgcaat
     3001 atcgccggct tcaacaagcg cgtacgcgaa gcgaacgacg ccggcaagcc gatccgcgat
     3061 ccggtcatga cggccaaggc cgcgaacgat ccgacgatcg accagtcgca gatcccggac
     3121 ctcatgccgc tgccgtacat cgtcgtgatc atcgacgagc tcgcggacct gatgctgatc
     3181 gtcggcaaga aggtcgagga gctgatcacg cgcctcgcgc agaaggcgcg cgcc
//
LOCUS       DAOQVY010000177         3114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_36265_length_3114_cov_4.104609, whole genome shotgun sequence.
ACCESSION   DAOQVY010000177 DAOQVY010000000
VERSION     DAOQVY010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36265_length_3114_cov_4.104609"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PKN91_11935"
     CDS             complement(<1..244)
                     /locus_tag="PKN91_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28819.1"
                     /translation="MRAKTLSSKGNAGAGGTFGTALIVGLLGAGLALAQDAESPVGET
                     QQASREVRVGLEEVMVTARRREESMMDVPIAITALTT"
     gene            complement(669..2402)
                     /locus_tag="PKN91_11940"
     CDS             complement(669..2402)
                     /locus_tag="PKN91_11940"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNS28820.1"
                     /translation="MLLGAWRERVSRARAHLGWADTPTPRYTGRAAIEPIASRVYQGG
                     ASLALAAPIDQLYTATELNEWALAASVFERDPARWATLEDQMVQVASQASTPPALPPV
                     LDEQAALERLLRLASLEANPSLHSVIDRAAARGLGWLYDDEGLTLGEGAGHGNFLASA
                     VPRTDNVRWEALRNVPTALVTGSNGKTTTVRLLAACAQAQGWHAGYNCTDGVFLGGRL
                     AIGGDFSGPVGARAVLRDSRVEAAIIEAARGGILRRGLAPNRADVAVVTNVSPDHFGT
                     YGIEDLESMAATKLVVANVVEPRGLVVLNADDPTLVHGAARIEAPVGWFALDADVPLL
                     REHRMRGGATCGLRDGRLIVAHGGSEHDLGDIRAMPLTMDGSARYNIANIAGAALAAL
                     ALGVGTGVVRTVLARFGSSPADNPGRLMRLDVSGLRIVLDYAHNPEGLRGVLGVAQHL
                     RAGGRLIMILGQAGDRDDDDIAELAATAAAGHPDLVVIKETESYLRGRAPGEVPALLR
                     ASLLRHGLGGAQLIDCPSEIEAVRSALGVARPGDVLLLPVHGYLARGEVVSLIGRLQR
                     EGWSAGEPLPL"
     gene            complement(2513..>3114)
                     /locus_tag="PKN91_11945"
     CDS             complement(2513..>3114)
                     /locus_tag="PKN91_11945"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNS28821.1"
                     /translation="FQAPGRMNVFNEHPFKVLFDYGHNAHAVSMLADLAQRLDVTGRR
                     IVVLAGPGDRRDEDLKAIARAVAGRFDHYICRRDDTLRGRDGDEVPRILATTLREAGV
                     PAAQISQIPDEQEAIDAALRMGTKGDLLLVFADQLTRSWKQITKFRPGEVAEPVAAPA
                     PERAVPVPEPAAAAVAVDIALEGLIRDERGIRFAPEGED"
BASE COUNT          511 a         1083 c         1001 g          519 t
ORIGIN      
        1 cggttgtcag tgccgtgatc gcgatcggca cgtccatcat gctttcctcg cgcctgcggg
       61 cagtaaccat gacctcttcg aggccgaccc ggacctcacg cgacgcttgt tgcgtttccc
      121 ccaccggaga ctcagcgtcc tgcgccaatg ccaatccggc tccaagtaag ccgacaatca
      181 atgccgtgcc gaacgtaccg ccggcgccag cattaccctt gctcgatagt gtcttcgcac
      241 gcattgacat cccctattgg cccggacccc ggatttatga cgacgaccta caagctccaa
      301 gtgttcaaga gctgattgtc cgttcttgtg actaaactag cgtagttatc taggtcggtc
      361 aagcgccgtc tgttgcgtag ttatctgggt cagtcctgct ggatgcggca cgcccgggat
      421 gcatcattga cggtgtgacg gaacgacttt ccgcctcggc ttttcgccgg aattccaatg
      481 agcaacgctc ggtcgcagaa ggggagactg acggtcctgt tggcactgca gcaggcgttg
      541 gtcaattggc tcctggatgc cacgaggctt cttgaggcga tcgtgcatcg tgttccgttg
      601 ttcggctgtt cggggataaa cagcccacgt tgagcacgac ttttcgcgaa gtcgccactg
      661 atggtgtgtc acagtggcaa cggctcgccg gcactccacc cttcccgctg aagcctgccg
      721 atcagcgaca cgacctcgcc gcgcgcgaga tagccgtgca ccgggagcag caagacgtcg
      781 cccggcctcg ccaccccgag cgccgagcgc accgcttcaa tctcggatgg gcaatcgatc
      841 aactgcgcgc cgccgaggcc atggcgcagg agactggcgc gcagcagggc tggtacctcg
      901 ccgggtgcgc ggccacgcag gtagctttcg gtctccttga tcacgacgag atccgggtgc
      961 ccggcagctg cggtggcggc gagttccgcg atgtcgtcgt cgtcacgatc cccggcctgt
     1021 ccaaggatca tgatgagcct gcccccagcg cgcagatgct gcgcaacgcc cagcacgccg
     1081 cgcaggcctt ccgggttgtg cgcatagtcg agaacgatgc gcaggccgct gacatcgagg
     1141 cgcatcagcc gccccggatt gtccgcgggg ctgctgccga agcgcgccag caccgtgcga
     1201 accaccccgg taccgacacc cagcgcgagc gcggcgagcg ccgcaccggc gatgttcgcg
     1261 atgttgtagc gcgcactgcc gtccatcgtc agcggcatcg cacggatgtc gcccaggtca
     1321 tgctcgctgc cgccgtgcgc gacgatgagg cgcccgtcgc gcagcccgca ggtcgccccg
     1381 ccgcgcatgc gatgttcacg caataatggg acatcggcat cgagtgcgaa ccagcccacc
     1441 ggtgcctcga tgcgtgccgc gccatgcacg agcgtcgggt cgtccgcatt cagcacgacg
     1501 agcccgcggg gttccacgac attcgccacg accagcttgg tcgccgccat cgactcgaga
     1561 tcttcgatgc cgtacgtgcc gaaatgatcc gggctcacgt tcgtgaccac tgccacgtcg
     1621 gcacgatttg gcgctagccc gcggcgcagg atgccgccgc gcgccgcctc gatgatcgcc
     1681 gcctccacgc gcgaatcgcg cagcaccgcg cgcgcgccca cagggccaga aaagtcgccg
     1741 ccgattgcga gcctgccgcc caggaatacg ccgtcggtgc agttgtagcc cgcgtgccaa
     1801 ccctgtgcct gcgcacaggc ggccaacagg cgcacggtag tggtcttgcc gttggatccg
     1861 gtgacgagcg cggttggaac gttgcggagg gcttcccaac gcacgttatc cgtccgcggc
     1921 acggcgcttg cgaggaaatt gccgtgtccg gcgccctccc cgagcgtcag gccctcatcg
     1981 tcgtacagcc atccgagccc gcgcgccgcc gcacggtcga tcaccgaatg cagcgacgga
     2041 ttcgcctcga gcgaggcgag acgcagcagg cgctcgagcg cggcctgctc atcgaggacc
     2101 gggggcaatg ccggcggcgt gctggcctgc gacgccacct gcaccatctg gtcctcgagc
     2161 gtcgcccagc gcgcggggtc acgctcgaat accgatgccg cgagcgccca ctcattgagc
     2221 tcggttgccg tgtacagctg gtcgatcggg gccgcgagcg cgagcgatgc tccgccctga
     2281 tacacgcggc tcgcgatggg ctcaatcgcg gcgcggccgg tgtagcgcgg tgttggggtg
     2341 tcggcccagc ccaggtgagc ccgggcccgc gacacgcgct cacgccatgc accgagcaac
     2401 atgggatcga tcccgatccc cacgacatcg agcacggcgc cgggagcatc gaagaaaagg
     2461 ttgctgccgg tgagtcgccg cgagttgtcg aacggcagtg caacccgatc gatcagtcct
     2521 ctccctccgg ggcgaaccgg atgccgcgct catcccggat caggccctcg agtgcaatat
     2581 ccactgctac tgccgcggcc gcaggctccg gcacgggcac ggcgcgctca ggtgccggcg
     2641 cggccacggg ttccgcgact tcgccgggac ggaacttggt gatctgcttc cacgagcgcg
     2701 tcaactggtc cgcgaaaacc agcagcaagt cgccctttgt gcccatgcgc agcgctgcat
     2761 cgatggcttc ctgttcatcg gggatctgcg aaatctgcgc cgcaggcaca ccggcctcgc
     2821 gcagggtggt cgcgaggatc cgcggcactt catcgccgtc ccgaccacgc agcgtgtcgt
     2881 cgcggcggca gatgtaatgg tcgaagcggc ccgccaccgc gcgcgcgatg gccttcaggt
     2941 cctcgtcgcg gcgatcgccc ggcccggcca gcaccacgat gcggcgcccg gtcacgtcga
     3001 gccgctgggc gaggtcggcc agcatcgata ccgcgtgcgc attgtggccg tagtcgaaca
     3061 gcaccttgaa cgggtgctcg ttgaacacgt tcatgcgccc gggcgcctgg aaga
//
LOCUS       DAOQVY010000178         3106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_36410_length_3106_cov_5.678466, whole genome shotgun sequence.
ACCESSION   DAOQVY010000178 DAOQVY010000000
VERSION     DAOQVY010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36410_length_3106_cov_5.678466"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1976)
                     /locus_tag="PKN91_11950"
     CDS             complement(<1..1976)
                     /locus_tag="PKN91_11950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNS28822.1"
                     /translation="MPARGRCLVSVVFALAFTSVHAEGTAPFTSEAMWGLARLGAPTL
                     SPDGAHAVLPVTRYDLDENKGDADLWIVPTAGGAARQFTSGKANDSSPVFSPDGQWIA
                     FLSKRDDDEQNQIYVIAVNGGEARRVTEIPVGVAAIKWFPDSRRIAFVSNVWPDLVKW
                     EDQGKRQKERADAKTSARVWDKAPIAYWDHLLDDRERHLFSISIDGGETTAITRGSGF
                     WLSKQEVGPGSYDISPDGEEIAFIADRDRSGIDANDDVILIATCGCKPARNLTPDSVA
                     DEGSPVYSPDGRYLAYTQARTPRFYADTRRLMLRDRASGATRGLTEDWDRSAGNLIWA
                     PDSRSLYGEIDDAATQRIYQFPLAGGKPRAVTAASSFSDLDISGRTAIALRQNFSEPA
                     TLVRVDLAKGSATKLSDFNDGALAGFALGKVESVTYKGANDADIQMWVIYPPGFDPAK
                     KYPVYMLLHGGPHNAILDAVTYRWNAHVFASWGYVVTWHNFHGSSGFGQDFADSINPD
                     RISLPYEDTIKAATWLAAQPWVDGERMVAGGGSYGGFLAATLLGRAHPFKALIAHAAV
                     YNNFTQVAADYGASKERFFSFWEKPEEFARYSPHTSAGNFNTPTLVIHGQLDQRVPVN
                     HGIELFNTLQTRGVPSKLVYFPDENHWVLKPGNSL"
     gene            2111..>3106
                     /locus_tag="PKN91_11955"
     CDS             2111..>3106
                     /locus_tag="PKN91_11955"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNS28823.1"
                     /translation="MSPARLDRYCAVALQIDCDSVHPDSDRASARARMQRTLARIDAA
                     ISGAKRWIGADLRLVVLPEYILTGPPWGETIAEWADKAAIDEDGPEYAAFAQLAQRHG
                     VHLAGNVYETDRNFPGLYFQASFAFDDAGDKVLRYRRLVSMFAPTPHDVWDRYLDLYG
                     LEGVFPVADTVLGRLAAIASEEILYPEIARCHAIRGAEVFLHCSSEVAAVTLTPKQIA
                     RRARALENLAYVVSANNAAVHGIDVPADSSNGFSDVIDYMGNQIVQAGAGESMVANAS
                     LDLAGLRAWRRRPGMGNMLSRQALDLFASSYAAAGIREANGLLDGGAIVVPDRDFF"
BASE COUNT          472 a         1024 c         1076 g          534 t
ORIGIN      
        1 agcgaattgc ccggcttcag cacccagtgg ttctcgtcgg ggaagtacac gagcttcgag
       61 ggtacgccgc gggtctgtag cgtgttgaac agctcgatgc cgtggttcac cggtacgcgc
      121 tggtcgagct ggccgtggat caccagcgtc ggcgtattga aattgcccgc tgaagtgtgc
      181 ggcgagtagc gcgcgaactc ttccggcttc tcccagaaac tgaagaaccg ctccttcgac
      241 gcgccgtagt cggcggcgac ctgcgtgaag ttgttgtaga cggccgcatg ggcgatcagc
      301 gccttgaaag ggtgcgcccg cccgagcagc gtggcggcga ggaagccgcc gtagctgccg
      361 ccgccggcga ccatgcgctc gccgtcgacc cagggttgcg cggcgagcca ggtggccgcc
      421 ttgatcgtgt cctcgtaggg cagcgagatc ctgtcggggt tgatcgagtc ggcaaagtcc
      481 tgcccgaagc cgctcgagcc gtggaagttg tgccacgtga cgacgtagcc ccagctcgcg
      541 aatacgtgcg cgttccagcg ataggtgacc gcatccagga tcgcgttgtg cgggccgccg
      601 tgcagcagca tgtagacggg gtacttcttt gccgggtcga agccgggtgg gtagatgacc
      661 cacatctgga tgtcggcatc gttcgcaccc ttgtaggtga cgctctcgac cttgccgagc
      721 gcgaagccgg cgagcgcccc gtcgttgaag tcggacagct tggtcgcgct gcccttcgcg
      781 aggtccacgc gcacgagcgt cgccggctcg gagaagttct ggcgcagcgc aatcgcggtc
      841 ctgccgctga tgtcgaggtc ggagaaactg ctcgcggcgg tcaccgcgcg cggcttgccg
      901 cccgcgagcg ggaactggta gatgcgctgc gtcgccgcgt cgtcgatctc gccatacagc
      961 gagcgcgagt cgggcgccca gatgaggttg ccggcggagc ggtcccagtc ctcggtcagg
     1021 ccgcgcgtcg cgccgctggc gcgatcgcgc agcatcagtc ggcgcgtgtc ggcgtagaag
     1081 cgcggcgtgc gcgcctgcgt ataagcgaga tacctgccgt cgggcgagta caccggactg
     1141 ccctcgtcgg cgacgctgtc gggtgtcagg ttgcgtgccg gtttgcagcc gcaggtggcg
     1201 atcaggatga cgtcgtcgtt cgcatcgatg ccgctcctgt cgcggtcggc gatgaacgcg
     1261 atctcttcgc cgtcgggcga gatgtcgtat gagcccgggc ccacttcctg cttcgacagc
     1321 cagaagcccg agccgcgcgt gatcgcggtg gtctcgcccc cgtcgatcga aatcgagaac
     1381 agatgacgct cccggtcgtc gaggagatgg tcccagtagg cgatcggcgc cttgtcccag
     1441 acccgcgcgc tcgtcttggc gtcggcacgc tccttctgcc gcttgccctg gtcctcccat
     1501 ttgacgaggt cgggccacac gttgctcacg aaggcgatgc gacggctgtc cgggaaccac
     1561 ttgattgcgg cgacgccgac cgggatttcg gtcacgcgcc gcgcctcgcc gccattgacg
     1621 gcgatcacgt agatctggtt ctgctcgtcg tcgtcacgct tggagaggaa tgcgatccac
     1681 tggccgtccg ggctgaacac cggcgagctg tcgtttgcct tgccgctggt gaactggcgc
     1741 gccgcgccgc cggcggtcgg gacgatccac aggtccgcgt cacccttgtt ttcgtcgagg
     1801 tcgtagcggg tgaccggcag caccgcgtgc gcgccatcgg gcgagagtgt cggcgcaccg
     1861 agccgcgcga ggccccacat ggcctcgctg gtgaacggag cggtgccttc ggcgtgaacg
     1921 gaagtgaagg ccagggcgaa gacgacggaa accaggcagc ggccgcgggc aggcatagtc
     1981 gaaaccccca tgttcatggt gcgctcgttg cgcgcgatag cagcggattg tgccccatcc
     2041 ggccgctcgc cgccgccaat ctgcgacgaa cgccgcgtac aatccgcggc agttccgccg
     2101 aggtatccac atgagcccag cccgactcga ccgctactgc gcggtggccc tgcagatcga
     2161 ctgcgacagc gtgcatcccg acagcgaccg cgccagcgcg cgcgcgcgca tgcagcgcac
     2221 gctcgcacgc atcgatgccg cgatatccgg cgccaagcgc tggatcggcg cggacctgcg
     2281 actcgtcgtg ctgccggagt acatactcac cggcccgccc tggggcgaga cgatcgcgga
     2341 gtgggcggac aaggccgcca ttgacgagga cgggcccgag tacgcggcgt tcgcgcagct
     2401 cgcgcagcgc cacggcgtgc atcttgccgg caatgtctac gagaccgacc ggaatttccc
     2461 cggactctac ttccaggcct cgttcgcctt cgacgacgcc ggtgacaagg tgctgcgcta
     2521 ccggcgcctc gtgtcgatgt ttgcgccgac gccgcacgac gtgtgggatc gctatctcga
     2581 tctctacggt ctcgaaggcg tcttcccggt cgccgacacc gtactcggcc gactcgcggc
     2641 gatcgcgtcc gaggaaatcc tctatccgga gatcgcgcgc tgccatgcga tccgtggcgc
     2701 cgaggtgttc ctgcactgct cgagcgaagt ggcggcggtg actctgacgc cgaagcagat
     2761 cgcgcgccgc gcgcgtgcgc tcgagaacct ggcttacgtc gtttcggcga acaacgcggc
     2821 agtgcacggc atcgacgtgc cggccgattc gagcaacggg ttctccgacg tgatcgacta
     2881 catgggcaac cagatcgtgc aggcgggtgc gggcgagtcg atggtggcca acgcgtcgct
     2941 cgacctcgcg ggcctgcgcg cctggcggcg gcgacccggc atgggcaaca tgctgtcgcg
     3001 ccaggcgctc gacctgttcg cgtcgagcta cgcggcggcg ggtatccgcg aggcaaacgg
     3061 attgctcgac ggcggtgcca tcgtggttcc cgatcgcgac ttcttc
//
LOCUS       DAOQVY010000179         3086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_36773_length_3086_cov_5.475751, whole genome shotgun sequence.
ACCESSION   DAOQVY010000179 DAOQVY010000000
VERSION     DAOQVY010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36773_length_3086_cov_5.475751"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PKN91_11960"
     CDS             complement(<1..324)
                     /locus_tag="PKN91_11960"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HNS28824.1"
                     /translation="MCANSLPRRPVGFTIWELATVMAVVAALLAAGAPGFARLRAAAG
                     VTTNADRLMGAMHFARSQAILTGRPFVVCLSADLASCVGRGAQSGRAWIVFENAHAEF
                     TPVRDA"
     gene            403..816
                     /locus_tag="PKN91_11965"
     CDS             403..816
                     /locus_tag="PKN91_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNS28825.1"
                     /translation="MPNKEIARRLEAHGILPTAQRLRIAGILLAAPQHMTAEQLLAQL
                     QEAGARVSKATVYNTLNLFADRGLIRPLSVDGSRAWFDSNVEAHYHFHDVDSGTLLDV
                     GVPEVGFSRLPAVPDGMEVAGIDVVIRLRKAAPRN"
     gene            864..939
                     /locus_tag="PKN91_11970"
     tRNA            864..939
                     /locus_tag="PKN91_11970"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:897..899,aa:Thr,seq:cgt)
     gene            complement(974..1279)
                     /locus_tag="PKN91_11975"
     CDS             complement(974..1279)
                     /locus_tag="PKN91_11975"
                     /inference="COORDINATES: protein motif:HMM:NF009508.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI-like protein"
                     /protein_id="HNS28826.1"
                     /translation="MHYLLFYEFVPDYLERRQPLRAGHLSLAWAAHDRGELVLAGAFG
                     GDPPGSALLFRGESPAAAEQFAKADPYVTAGLVTRWWVRPWTTVVGETAANPLRPEP"
     gene            1393..2742
                     /locus_tag="PKN91_11980"
     CDS             1393..2742
                     /locus_tag="PKN91_11980"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNS28827.1"
                     /translation="MRRILSGTCLAIALSVTTPALTASPDHFALVHAGTLLAVPGRAP
                     EREMTIIVKNDRIVGVRHGYLDAAAAGLGSGADAEVIDLRDRYVLPGLMDAHVHLIDE
                     PSFGRRSSGRGNRAGRTPAQAAVNAMVYARRTLAAGFTTVRDLGSDDQAVFAVRDAIE
                     AGRMIGPRILVAGSALAVTGGHGDSLPLDATGDPHSRLHDGTCDGASECRQAVRYQYK
                     LGADVIKFTSTGGFSWNTGLEPQLFPDEIEAIVSTSHLVGLKAAAHAYSATSIKQAVR
                     AGVDSIEHGFLVDDEGLSLMRKAGTYLVPTLSASYPPPIFRIPNPESVRIRGEHRAFE
                     RALAAGVRIAFGTDAGTFAHGDNAKEFGLMVKFGMQPMDAIRSATITTADLFGLSADV
                     GTLEAGRVADLIALAGDPLEDVARLLQVDFVMKSGRIAKQGGRMTAPFSHPPPSADF"
     gene            2864..>3086
                     /locus_tag="PKN91_11985"
     CDS             2864..>3086
                     /locus_tag="PKN91_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28828.1"
                     /translation="MKLVTSGSMLSVALAALLVAQGCAKKESAETTQAASGPEAAAVA
                     EPMVAADEPAPTTDAADAAPDAAAAPATEA"
BASE COUNT          518 a         1049 c         1030 g          489 t
ORIGIN      
        1 ggcatcgcgc accggcgtga attcggcgtg cgcattttcg aacacgatcc aggcccgccc
       61 cgattgtgcg ccgcgaccga cgcaactggc caggtcggcc gacaggcaga ccacgaacgg
      121 cctgccggtc aggatggcct ggctgcgggc gaaatgcatc gcgcccatga gccggtcggc
      181 gttggtcgtg acgccggccg ctgcgcgcag ccgcgcgaaa cccggtgctc cagcagccag
      241 cagggcagcc accacggcca tgacggtggc gagttcccag atggtgaaac cgaccggccg
      301 gcggggcagg gagttggcgc acatggcgaa aacatagtcc tcgccaacat cgcaaatgag
      361 tcgcatttgc gatgtaaatc cacagattcg tataattgcc ctgtgccgaa caaagagata
      421 gcccgccgcc tcgaagcgca cgggatcctg ccgaccgccc agcgcctgcg cattgcaggg
      481 attctgctgg ccgcgccgca acacatgacg gccgagcagt tgctcgcgca acttcaggag
      541 gctggcgcgc gcgtgtccaa ggcgaccgtc tacaacacgc tgaatctgtt cgcggaccgc
      601 gggctgatcc ggccactgtc ggtcgacgga tcgcgcgcct ggttcgactc gaacgtcgaa
      661 gcgcactacc acttccatga cgtcgacagc ggcacgctgc tcgacgtggg ggtgccggaa
      721 gtgggtttca gccgcctgcc cgccgtgccc gacggcatgg aagtggccgg aatcgatgtc
      781 gtaatccgcc tgcgcaaggc cgcgccgcgc aactagtgcg gtttgcctcg tcctggggca
      841 ttcattagaa ttcgcgcccc cgcgccgctt tagctcagct ggtagagcac atcattcgta
      901 atgatggggt cgtctgttcg aatcagacaa gcggcaccat ccacccccca cgcgtccatt
      961 tcctgcgtcc ggatcagggc tcgggacgca gcgggttcgc cgcagtctcg ccgaccaccg
     1021 tggtccacgg ccgcacccac cagcgggtga cgagcccggc ggtcacgtag gggtcggcct
     1081 tcgcgaactg ctcggccgcc gccgggctct cgcctcgaaa tagcaatgcg gaaccgggcg
     1141 ggtcgccgcc gaacgccccg gcgagcacca gctcgccgcg atcgtgcgcc gcccaggcga
     1201 gcgacaggtg tccggcacga agtggctggc ggcgttcgag gtaatcggga acgaattcgt
     1261 agaagagcag gtagtgcatg gggcctccgc gggcgattct aaccgcgtag ttccatcagg
     1321 cggtcgcgat cggcccgaga ctgaccgctg cggtgcgctt tgcgacgaag atggcgggcg
     1381 tggagggacc ccatgagacg aatcctgtcc ggaacctgcc ttgccatcgc actctcggta
     1441 acgacaccgg cgctgaccgc gtcacccgat catttcgccc tcgtgcacgc cggtacgctg
     1501 ctcgcggtgc cgggccgcgc acccgagcgg gaaatgacga tcatcgtgaa gaacgatcgc
     1561 atcgtcggcg tgaggcacgg atacctcgat gccgcggcgg ccgggctcgg gtccggcgcg
     1621 gacgccgaag tcatcgatct gcgcgatcgc tacgtgctgc ccggactgat ggacgcgcac
     1681 gtacatctga tcgacgagcc ttcgttcggt cggcgcagta gcggccgcgg caatcgcgcc
     1741 ggccgcacgc cggcgcaagc tgccgtcaat gccatggtgt acgcgcggcg cacgctcgcg
     1801 gcaggcttca cgaccgtgcg cgatctaggt tccgacgacc aggcggtgtt cgccgtgcgc
     1861 gatgcaatcg aggcgggcag gatgatcggc ccgcgtatcc tcgtcgcggg ctcggcgctc
     1921 gccgtcaccg gtggtcatgg cgattccctg ccgctcgatg caaccggcga cccgcactcg
     1981 cgcctgcatg acggcacctg tgatggcgcg agcgaatgcc ggcaggcggt gcggtaccag
     2041 tacaagctcg gcgccgacgt catcaagttc acgtccaccg gcggcttttc atggaacacg
     2101 gggctcgagc cccagctatt cccggacgag atcgaggcga tcgtctcgac cagccatctc
     2161 gtgggtctga aggcggccgc ccacgcctac tccgcgacct cgatcaagca ggcggtacgc
     2221 gcgggcgtcg actcgatcga gcacggcttt ctcgtcgacg acgaagggct ctcgctgatg
     2281 agaaaggccg gcacctatct cgtgccgacc ctgtcggcga gctacccgcc gccgatcttc
     2341 aggatcccga accccgagtc ggtacgcatc cgcggcgaac accgggcgtt cgagcgcgcc
     2401 ctggccgccg gcgtcaggat cgccttcggc acggatgccg gcacttttgc gcatggcgac
     2461 aatgccaagg agttcgggct catggtgaag ttcggcatgc agccgatgga cgcgatccgc
     2521 agtgcaacga tcacgacggc cgatctcttc ggactttcgg ccgacgtcgg cacgctcgag
     2581 gcgggcaggg tcgcggatct catcgcgctc gccggcgacc cgctcgaaga tgtggcccgg
     2641 ttgctgcagg tcgacttcgt gatgaagagc ggccgcatag cgaagcaggg cgggcgcatg
     2701 accgcgccgt tcagtcatcc accaccatcg gccgacttct gacggcggct cgcgcccgcc
     2761 tggcattccg ccctgtacgg tccggcggtt ttggggcaga cttctgctgc gcgcgtcgcg
     2821 acatttcccc cgcgcaccat cttccgaaag ccaggagcga gctatgaaac ttgtcacctc
     2881 gggttcgatg ctgtcggttg cgttggctgc actgcttgtc gcgcagggtt gcgcaaagaa
     2941 ggaatccgcc gagaccaccc aggccgcgag cggcccggag gctgccgcgg tggccgagcc
     3001 gatggtggcg gccgacgagc ccgccccgac gacagatgcc gccgacgcgg cgccggatgc
     3061 ggcggctgcc cccgcgaccg aagcac
//
LOCUS       DAOQVY010000180         3021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_37951_length_3021_cov_4.290290, whole genome shotgun sequence.
ACCESSION   DAOQVY010000180 DAOQVY010000000
VERSION     DAOQVY010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37951_length_3021_cov_4.290290"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1090)
                     /locus_tag="PKN91_11990"
     CDS             complement(95..1090)
                     /locus_tag="PKN91_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28829.1"
                     /translation="MLLFVTLAATLVLLAIIAVAVPLLRGPERPAPWAALGSVVVLLL
                     GSAVLYATWSNWTWQDASAHEAGDSISSLARRLERQPDDLEGWLQLGRSYAALEQFPL
                     ALRAYERADALAAGRNVEALVGMGEILAVMDDSQLVGRAGDLFERALELDPKAGKALF
                     YSAIAAMRRGELPKSRERFANLLALDPPDAVRPLIEQQVAALDRAIAGDEGGVAAPDT
                     QASVRVAVTASPDIAKGATADAPLFVIVRDPDAPGPPLAVKRVAAKFPLELVLTAADA
                     MMPNRAITAGKRVQVVARISRTGQPEAASGDPYGEVSYDVGKDGVREIVIDRVTP"
     gene            complement(1090..1530)
                     /locus_tag="PKN91_11995"
     CDS             complement(1090..1530)
                     /locus_tag="PKN91_11995"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNS28830.1"
                     /translation="MNARAFTLALVTSCLYAAALPAIDTTELPDPVLQERYETLTHEL
                     RCMQCQNQSIADSPVGLAADLRREVADQLVAGKTDAEIRDYMAARYGDFILFRPRLSA
                     RTAWLWALPGVLLLAGGVIAWRIVRQRSRLAANDPDFNADDPER"
     gene            complement(1527..2051)
                     /locus_tag="PKN91_12000"
     CDS             complement(1527..2051)
                     /locus_tag="PKN91_12000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HNS28831.1"
                     /translation="MNRFLLPLAAFALVAIVLGVGIRNVDTKGVVASPLIGRAAPAFV
                     LPNLLDPEAPFDSRSMAGRWYALNVWGTWCVECRVEHGTLVAARQAGQLPIIGLNWKD
                     DGASAIAWLAQLGDPYEATAVDAEGRVAIDFGVYGAPETFLIDPQGVVVHKHIGPLST
                     EVWERDFVARAAAK"
     gene            complement(2048..>3021)
                     /locus_tag="PKN91_12005"
     CDS             complement(2048..>3021)
                     /locus_tag="PKN91_12005"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HNS28832.1"
                     /translation="YAWRAPLLKSAAGFEFTSRESFLLFNNILLVIAATTVFGGTLAP
                     LISDTLGLGTLSVGTQYFNPTFLLPMLPMIALAAVGMHANWKRGRLAERSRPVVVTGL
                     IALAIALALVFGYYGGRPGLALVSLALGAWLVLSSLVDPIDRLRRGLSMPRAIVGMTL
                     AHIALGVFVISVTAVETYTTERDVALARGESIEVGGYDFRFDGIDDVHGPNYDGVHGI
                     VQVSREGRPIATLHPEKRRYFVQQSVMTEAGIESQAGRDLFVALGEDLGAGRWSLRIQ
                     VRPLINYVWLAALLMALGGCIAASDRRYRTARAPATVPVGAGQGSTA"
BASE COUNT          513 a         1036 c         1029 g          443 t
ORIGIN      
        1 ggccggtcaa cacgcgtgtg tagttggccc agatgcccgg cggactggcg aattggagtg
       61 tgaggcttgt agccatgaac ggtgatccgg cctgtcaggg cgtcacgcga tcgatcacga
      121 tctcccgcac gccatctttg ccgacatcat agctgacttc accatacgga tcgccgctcg
      181 cggcctccgg ctgcccggtg cgtgaaatgc gcgccacgac ctgcacgcgc ttgcctgcgg
      241 tgatggcgcg gttcggcatc atcgcgtccg ctgccgtcag tacgagttcc agcggaaatt
      301 tcgcagcgac gcgtttgact gcgagcggag ggcccggcgc atccggatcg cgcacgatca
      361 cgaacagcgg cgcgtcggcc gtggcgccct ttgcaatgtc gggtgacgcg gtgacggcga
      421 cacgcacgct cgcctgtgtg tcgggcgcgg caaccccgcc ctcgtcaccg gcaatggcgc
      481 gatcgagggc ggcgacctgc tgctcgatca gcgggcggac cgcatccggt ggatcgagcg
      541 cgagcaggtt ggcgaaacgc tcgcgggatt tcggcagctc gccgcggcgc atcgctgcga
      601 tcgcgctgta gaacaaagcc ttgccggcct tcgggtcgag ttcgagtgcg cgctcgaaca
      661 ggtcgcccgc gcgcccgacg agttgcgagt cgtccatcac ggcgagaatc tcgcccatgc
      721 cgaccagcgc ctcgacattg cggcccgcgg cgagcgcatc ggcacgttca taggcgcgca
      781 gcgcgagcgg aaactgctcg agcgccgcgt atgaacgacc gagctgcagc cagccctcga
      841 gatcatcggg ctgccgctcg agccggcgcg cgaggctcga tatgctgtcg ccagcctcgt
      901 gggccgaagc atcctgccag gtccagttgc tccacgtggc gtaaagcacg gcgctgccca
      961 gcagcagcac cactacgctg ccgagcgccg cccacggcgc gggtcgctcc ggtccgcgca
     1021 gcagcggcac ggcgacggcg atgatggcga gcaggacgag cgtggcggcc agcgtcacga
     1081 acagcagcat cagcgctccg gatcgtcagc gttgaaatcg gggtcgttcg cggcgaggcg
     1141 cgatcgctgg cgcacgatgc gccatgcgat cacgcccccg gcgagcagca gcacgcccgg
     1201 caaggcccac agccatgccg tacgcgccga caggcgtggc cggaacagga tgaaatcgcc
     1261 atagcgggca gccatgtagt cgcgtatttc ggcgtcggtc ttgcccgcca cgagctgatc
     1321 ggcgacttcg cgacgcagat ccgcggcaag ccccaccggc gagtccgcga tcgactggtt
     1381 ctggcattgc atgcagcgca gctcgtgagt gagcgtctcg tagcgttcct gcagcacggg
     1441 atcgggcagt tccgtcgtgt cgatcgccgg cagcgctgcg gcatacaggc agcttgtcac
     1501 cagcgcgagc gtgaacgcgc gggcgttcat ttggccgccg cccgcgcgac gaagtcgcgc
     1561 tcccagacct cggtcgacag gggaccgatg tgcttgtgca cgacgacgcc ctgcgggtcg
     1621 atcaggaacg tctccggcgc gccgtagacg ccgaagtcga tcgcgacgcg gccttccgca
     1681 tccaccgccg ttgcttcata cggatcgccg agctgcgcga gccaggcgat ggcgcttgcg
     1741 ccgtcgtcct tccagttgag cccgatgatc ggcaactggc ccgcctgcct ggccgcgacc
     1801 agcgtaccgt gctccacacg gcactcgaca caccatgtgc cccacacatt gagcgcgtac
     1861 cagcgcccgg ccatggaacg cgaatcgaac ggagcctctg ggtcgagcag attcggcagc
     1921 acgaatgctg gcgcggcacg cccgatcagc ggcgaagcca cgacaccctt cgtgtcgacg
     1981 ttcctgattc cgacgcccag aacgattgcg accagcgcga aggccgcaag cggcagcagg
     2041 aagcgattca cgcggtactg ccctgccccg ccccgacagg cacggtggcc ggtgcacgcg
     2101 cggtccgata tcgccggtcg ctggccgcga tgcagccgcc gagcgccatc agcagcgccg
     2161 cgagccagac gtagttgatc agcggtcgga cctggatgcg caggctccag cgtccggccc
     2221 cgaggtcctc gcccagggcg acgaacaggt cgcgcccggc ctgcgattcg atgcccgcct
     2281 cggtcatcac cgactgctgc acgaagtagc ggcgcttctc cgggtgcagc gtggcgatcg
     2341 gtctgccttc acgcgaaacc tgcacgatgc catgcacgcc gtcgtagttc gggccatgga
     2401 cgtcgtcgat gccgtcgaag cggaaatcat agccgcccac ctcgatgctc tcgccgcgcg
     2461 cgagcgccac gtcacgctcg gtcgtgtagg tctccaccgc ggtcaccgag atcacgaata
     2521 cgccgagcgc gatgtgcgcg agggtcatgc cgacgatcgc gcgcggcatc gacagcccgc
     2581 gtcgcaggcg atcgatcggg tcgaccagcg acgacagcac gagccaggca ccgagcgcga
     2641 ggctgacgag cgcgagcccc ggccggccgc cgtagtagcc gaacacgagg gcgagtgcga
     2701 tggcgagcgc gatcagcccg gtcaccacga cgggccggct gcgctcggcg agccggccgc
     2761 gcttccagtt ggcgtgcatg ccgaccgcgg cgagcgcgat catcggtagc atcggcagca
     2821 ggaaggtcgg gttgaagtac tgcgtgccga ccgacagggt gccgagcccc agcgtatcgg
     2881 agatgagtgg tgcgagcgtg ccgccgaata ccgtggtggc cgcgatcacc agcaggatgt
     2941 tgttgaagag caggaacgat tcgcgtgacg tgaactcgaa gcctgccgcg gacttcagca
     3001 gcggcgcacg ccacgcatag a
//
LOCUS       DAOQVY010000181         2930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_39737_length_2930_cov_4.380174, whole genome shotgun sequence.
ACCESSION   DAOQVY010000181 DAOQVY010000000
VERSION     DAOQVY010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39737_length_2930_cov_4.380174"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /gene="sppA"
                     /locus_tag="PKN91_12010"
     CDS             complement(<1..1452)
                     /gene="sppA"
                     /locus_tag="PKN91_12010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNS28833.1"
                     /translation="MRAFFGALWRGLDGLRKFLHLVLLLLLFGIVVGALRTTIPVLPS
                     KAALVIRPEGQIVEQLTGDPFERAVERLQGGLRSETLLWDLTDAITAARDDARIGALV
                     LDLDRFTGAGQPTMQELTAAIGEFRASGKKVIAHGSLYLQAPYYLAAHADEIYVDPLG
                     FVLIDGYDRYVMYYRSALEKLGVDMNVFRVGAYKSAVERYTRTDMSAEDREESLAYLG
                     ALWSGYQGEVEKARQLPADAISTYVAGAAAALAAQGGDGAKLALDAGLVTGIRTSIEV
                     ENELAELVGRDDDNDSYRGVEVDEYLRVVRAEATLRRDGKPVIGVVVAAGEILDGFQP
                     AGTVGGESTARLIREARLDDNVKAVLLRIDSPGGSMFAAEQIHREIEALKAEGKPVIV
                     SMGDVAASGGYYIAAPADEIWAQRTTITGSIGIFAAIPTFNRTLDKIGVSVDGVGTTP
                     LSGQLRLDRPLGKEAGQVLQATVEHGYGVFLDRV"
     gene            complement(1488..1832)
                     /gene="rplS"
                     /locus_tag="PKN91_12015"
     CDS             complement(1488..1832)
                     /gene="rplS"
                     /locus_tag="PKN91_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNS28834.1"
                     /translation="MANIIQQIEQSRMTREVPAFRPGDTVVVQVKVVEGDRERVQAYE
                     GVVIAISNRGLNSSFTVRKISHGEGVERVFQTHSPAIAGIQVKRRGHVRRAKLYYLRD
                     LSGKAARIAEKL"
     gene            complement(1807..2568)
                     /gene="trmD"
                     /locus_tag="PKN91_12020"
     CDS             complement(1807..2568)
                     /gene="trmD"
                     /locus_tag="PKN91_12020"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNS28835.1"
                     /translation="MQIEVVTLFPDMIRHALGFGVVGRAIERGLVQVGTEDPRAHTHD
                     VHRTVDDRPYGGGPGMVLKPGPMAAAIDAAAARAPRGAPRVYLSAQGEPLSQRLAMEL
                     AACAGLVLVAGRYEGLDERVITSRIDREVSLGDFVLSGGEVPALAVIDAVARLLPGVL
                     GDARSSAEDSFSDGLLDWPHYTRPVEFEGQAVPEVLQQGNHADIRRWRLKQAVARTWR
                     RRPDLIARGALSREASALLNEFLEEERHGQHHSAD"
     gene            complement(2585..>2930)
                     /gene="rimM"
                     /locus_tag="PKN91_12025"
     CDS             complement(2585..>2930)
                     /gene="rimM"
                     /locus_tag="PKN91_12025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNS28836.1"
                     /translation="ALDLDHGARESRRLVGVDSRDDAARLTGAVIEVEREALPDPGEK
                     QHYWVDLIGLAVRNVEGEALGRVDHFVEAPANDVMVVRGEREHWVPVTPQHLVKVDRA
                     AGTIVVDWPADF"
BASE COUNT          455 a         1055 c          949 g          471 t
ORIGIN      
        1 aacgcgatcg aggaacacgc catagccgtg ctcgacggtc gcctgcagga cctggcctgc
       61 ttccttgccg agcggccgat cgagccgcag ctggcccgac aagggggtgg tgcccacgcc
      121 gtcgaccgag acgccgatct tgtcgagcgt gcgattgaag gtcgggatcg ccgcgaagat
      181 gccgatcgag ccggtgatcg tggtgcgttg cgcccagatc tcgtcggccg gcgcggcgat
      241 gtaatagcca ccggaggcgg cgacgtcgcc catcgagacg atgacgggct tgccctcggc
      301 cttgagtgcc tcgatctcgc ggtggatctg ctcggcggcg aacatgctgc cgccgggact
      361 gtctatgcgc agcagcacgg ccttgacgtt gtcgtcgagt cgcgcctcgc ggatcagccg
      421 cgccgtcgat tcccctccga ccgtgccggc cggttggaag ccatcgagta tctccccggc
      481 ggcgaccacc acgccgatga ccggcttgcc gtcgcgcctg agcgtggcct cggcgcgcac
      541 gacgcgcagg tattcgtcga cttcgacgcc gcggtagctg tcgttgtcgt catcacgacc
      601 gacgagttcg gcgagctcgt tctcgacttc gatcgaggtg cggatcccgg tgacgagccc
      661 ggcgtcgagc gcgagtttcg ccccatcgcc gccctgcgcg gccagggcgg cggcggcacc
      721 tgcgacatag gttgaaatgg cgtcggccgg cagctggcgc gccttctcca cttcgccctg
      781 gtagccgctc cacagcgcgc cgagataggc gagcgactcc tcgcgatcct cggccgacat
      841 gtcggtgcgt gtatagcgct cgaccgcgct cttgtaggca ccgacacgaa acacgttcat
      901 gtcgacgccg agcttctcga gcgcgctgcg gtaatacatc acgtagcggt cgtagccatc
      961 gatcagcacg aagccgagcg ggtcgacgta gatttcgtcc gcatgggcgg cgaggtagta
     1021 cggtgcctgc aggtagagcg agccgtgcgc gatcaccttc ttgccggacg cgcggaactc
     1081 gccgatcgcc gcggtgagct cctgcatcgt cggctggccg gcgccggtga accggtcgag
     1141 atcgagcacc agcgccccga tgcgggcatc gtcgcgggcc gcggtgatcg cgtcggtcag
     1201 gtcccagagg agcgtttcgc tgcgcagccc gccctgcagc ctctcgactg cgcgctcgaa
     1261 cggatcaccg gtgagttgct cgacgatctg tccctcgggc cggatcacga gcgcggcctt
     1321 cgagggcagt accgggatcg tcgtgcgcaa agcgcccacg acgatgccga acagcagcag
     1381 caggagcacc aggtgcagga acttgcgcag cccgtcgaga ccgcgccaca aagcgccgaa
     1441 gaatgcccgc acggcgactc tccctcagga agcggaggac ttccgcgtca gagcttctct
     1501 gcgattctcg cggccttgcc cgacaggtcg cgcaggtaat agagcttggc gcggcgcacg
     1561 tggccgcgac gcttcacctg gatccccgcg atcgccgggc tgtgggtctg gaacacgcgc
     1621 tccacaccct cgccatggct gatcttgcgc acggtgaacg aggagttgag gccgcgattg
     1681 ctgatcgcga tcaccacacc ctcgtaggct tgcacgcgct cgcggtcgcc ttcgaccacc
     1741 ttcacctgca cgactacggt gtcccccgga cggaatgccg ggacctcgcg ggtcatgcgg
     1801 ctctgttcaa tctgctgaat gatgttggcc atgtcgttct tcctcgagaa actcgttcag
     1861 gagcgcgctc gcctcgcggc tgagtgcgcc cctcgctatc aaatccggcc ggcgtcgcca
     1921 cgtgcgcgcc accgcctgct tcaaccgcca gcgccggatg tcggcgtggt tgccctgctg
     1981 cagaacctcg ggcaccgcct gcccctcgaa ctccaccggc cgcgtgtagt gcggccaatc
     2041 gaggagtccg tccgaaaacg aatcctcggc gctcgaacgc gcatcgccca gcacccccgg
     2101 caggagccgc gccaccgcgt cgatcaccgc cagcgccggc acttcgccgc ccgacagcac
     2161 gaagtcgcca agcgatactt cgcggtcgat gcgcgatgtg atcacgcgct cgtccagtcc
     2221 ttcatacctg cctgcgacga gcaccagccc ggcgcaagcc gccagttcca tcgcgagccg
     2281 ctgcgagagc ggttcgccct gcgccgagag atacaccctc ggtgccccgc gcggcgcgcg
     2341 cgccgctgcg gcatcgatcg ccgccgccat cggcccgggc ttcagcacca tgccgggtcc
     2401 gccgccgtat ggccggtcgt cgaccgtgcg atgcacatcg tgcgtgtgcg cgcgcggatc
     2461 ctccgtgccc acctgcacga gcccgcgctc gatcgcgcgc ccgacgactc cgaagccgag
     2521 cgcatgccgg atcatatccg ggaacagcgt aacgacttcg atctgcattg cccccgaccg
     2581 ccggtcagaa atccgcgggc cagtcgacga caatcgtgcc ggcggcgcga tccaccttca
     2641 cgaggtgctg cggcgtgacc ggcacccagt gctcgcgctc gccgcgcacc accatcacat
     2701 cattcgctgg cgcctcgacg aaatgatcga cgcgcccgag tgcctcgcct tccacgttgc
     2761 gcaccgcaag gccgatcagg tcgacccagt agtgctgctt ctcgccggga tccgggagcg
     2821 cctcgcgctc gacctcgatc acggcgcccg tgagtcgcgc cgcgtcgtcg cgcgaatcga
     2881 ccccgacgag gcggcgcgac tcacgggcgc cgtgatcgag gtcgagcgcg
//
LOCUS       DAOQVY010000182         2904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_40265_length_2904_cov_5.778519, whole genome shotgun sequence.
ACCESSION   DAOQVY010000182 DAOQVY010000000
VERSION     DAOQVY010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40265_length_2904_cov_5.778519"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PKN91_12030"
     CDS             complement(<1..995)
                     /locus_tag="PKN91_12030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HNS28837.1"
                     /translation="MIVKSRVLALALLFLPQLALALGLGDIRLNSRLNSPLDAEIELV
                     GATAEELSTLKAQLASRETFARYALEWPAFLSGVTVDQVRSADGRTLLRLRSQDPVTE
                     PFVTMLVEVNWARGRLVREYTVLIDPPIFAPGDAQAAAPVAAPTVGAQRQGEIARPAP
                     TPSGAAAPSASRAPVAAGGADGSYTVRRGDTLTSIAAGLTGGADTQRMMVGLYQGNPG
                     AFGNNMSDLHAGAVLRVPDAATLAAISASDAAAEVRRQYAAWSGRSGAAVGEERLRLV
                     PPSQAGTASGSGAAASAELEQASARVRQLESELQEARRQIEIRNTQLADLQARLAA"
     gene            complement(1096..2118)
                     /locus_tag="PKN91_12035"
     CDS             complement(1096..2118)
                     /locus_tag="PKN91_12035"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNS28838.1"
                     /translation="MSRKFRVAVVGATGLVGDTMIKVLEERDFPVSELYPLASSRSLG
                     KSVEFRGKEYPVLELEKFDFSRADIGLFSAGGSVSREYAPKAWAAGCIVIDNTSEFRY
                     EDDIPLVVPEVNPQDIARYRTRGIIANPNCSTIQMVVALKPLHDAAGIERINVATYQS
                     VSGAGKEAVEELAAQSAALLSGREAQAAVIAKQIAFNCVPQIDSFEANGYTREEMKLV
                     WETRKILGDDAVRVNATAVRVPVFYGHSEAVHIETRRKLSATEARELLERAPGVAVLD
                     EPVPAGYPTAVTEAANQDTVYVGRIREDISCDRGLDLWIVADNVRKGAATNSVQIAEI
                     LARDFL"
     gene            complement(2115..>2904)
                     /gene="aroC"
                     /locus_tag="PKN91_12040"
     CDS             complement(2115..>2904)
                     /gene="aroC"
                     /locus_tag="PKN91_12040"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNS28839.1"
                     /translation="DRFRPGHADYTYWQKYGVRDYRGGGRSSARETVMRVAAGAIARK
                     YLAVRLGVRIHGYLSRIGALELAPVDPAFAYRNPFFCPDPARVAELEALVWALRREGD
                     SIGARVTVVASGVPPGLGEPVFDRLDADIAGALMGINAVKAVEIGAGTQAAAQRGSEH
                     RDLLTPQGFKSNHAGGVLGGISSGQDIVAHLTLKPTSSIQVPGETIDLAGRPVEIVTT
                     GRHDPCVGLRATPIAEAMLAIVLMDHYLRHRGQNGDVVSTTPVI"
BASE COUNT          440 a         1032 c          966 g          466 t
ORIGIN      
        1 gccgcgaggc gcgcctgcag gtcggctagc tgggtgttgc ggatctcgat ctgcctgcgc
       61 gcttcctgca gctcgctctc gagctggcgc acgcgcgcgc tcgcctgctc gagctccgca
      121 ctcgccgcgg cgccgcttcc cgaagcggtg cccgcctgcg acggcggcac gagacgcagg
      181 cgctcctcac cgaccgccgc gccgctgcgc ccgctccacg ccgcatactg gcgacgcacc
      241 tcggcggcgg cgtcgctcgc actgatcgcg gccagcgtcg cggcatcggg aacgcgcagc
      301 acggcacccg catgcaggtc gctcatgttg ttgccgaagg cgccgggatt gccctggtag
      361 aggcccacca tcatgcgctg ggtgtccgcg cctccggtga ggcccgcggc aatggaggtg
      421 agcgtgtcgc cgcggcgcac ggtgtagctg ccatcggcgc cgcctgcggc cactggcgcg
      481 cgcgaggcgc tcggcgcagc cgccccgctc ggtgtcggcg caggccgcgc gatctcgccc
      541 tggcgctgcg ccccgacggt cggcgccgcg acaggcgccg ccgcctgcgc atcgcccggc
      601 gcaaagatgg gcgggtcgat gagtacggtg tattcgcgca ccaggcgccc gcgcgcccag
      661 ttcacctcga ccagcatggt gacgaacggc tcggtgacgg ggtcctgcga gcgcaggcgc
      721 agcagcgtgc gcccgtcggc gctgcgcacc tggtccacgg tgacgccgga gaggaacgcc
      781 ggccattcca gcgcgtagcg cgcgaaggtc tcccgcgagg ccagctgggc cttgagcgtc
      841 gagagttcct cggcggtcgc cccgaccagc tcgatctcgg cgtcgagtgg cgagttcagg
      901 cgcgaattga ggcgtatatc gcccagtccc agggcgagcg ccaactgcgg caagaacagc
      961 agggcaagtg ccagcacccg cgatttaaca atcattctga ctccccgtcc ggctcggcgc
     1021 agcgattttc acaattaaaa tcagcaagtt atgccagaaa tctcggctgc cacccgcatg
     1081 gcaccgcatt atgactcata aaaagtcgcg cgccaaaatc tccgcaatct gcacgctgtt
     1141 ggtggcggcg cccttgcgga cgttatcggc gacgatccag aggtcgagtc cccggtcgca
     1201 cgaaatatcc tcgcggattc tgccgacata aactgtgtcc tggttcgctg cctcggtcac
     1261 ggccgtcgga tacccggccg gtacgggctc atcgagcacg gccacccccg gcgcgcgctc
     1321 cagcaattcc cgcgcctccg tcgccgacag cttgcggcgt gtctcgatgt gtacggcctc
     1381 ggaatggccg tagaagaccg gcacgcgtac cgccgtcgca ttcacgcgca cggcgtcgtc
     1441 gccgaggatc ttgcgggttt cccacaccag cttcatttct tcgcgcgtgt agccattggc
     1501 ctcgaagctg tcgatctgcg gcacgcagtt gaacgcgatc tgcttcgcga tcaccgccgc
     1561 ctgtgcttcg cggcccgaca gcagcgcggc actttgcgcg gcaagttcct ccacggcctc
     1621 cttgcctgcg ccggacaccg actgataggt cgcgacgttg atgcgctcga tcccggcggc
     1681 atcgtgcagc ggcttgagcg ccaccaccat ctggatggtc gagcagttcg ggttcgcgat
     1741 gatgccgcgc gtcctgtagc gggcgatgtc ctgcgggttg acctcgggca cgaccagcgg
     1801 gatgtcgtcc tcgtaacgga actcggaagt gttgtcgatc acgatgcagc cggctgccca
     1861 ggccttcggc gcatactcgc gcgagaccga gccgccggcc gagaacaggc cgatgtcggc
     1921 gcgcgagaag tcgaacttct cgagctcgag caccgggtac tccttgccgc ggaactcgac
     1981 cgacttgccg agcgaacggc tgctcgcgag cgggtacagc tcgctgacgg ggaaatcgcg
     2041 ctcttccagg accttgatca tggtgtcgcc gacgaggccc gtcgcgccca ccactgccac
     2101 acgaaatttt ctgctcatat taccggtgtc gttgatacca catcgccatt ctgcccgcga
     2161 tgacgcaggt aatggtccat cagcacgatc gccagcatcg cctcggcgat cggcgtcgca
     2221 cgtaacccga cgcacgggtc gtgtcgaccc gtggtcacga tctcgacggg ccgccccgcc
     2281 agatcgatcg tctcgcccgg cacctggatg ctcgaggtcg gcttgagcgt caggtgcgcg
     2341 acgatgtcct gcccgctcga gatgccgccg agcacgccgc cggcatggtt cgacttgaag
     2401 ccctgcggcg tcagcaggtc gcggtgctcg ctgccgcgct gtgccgcagc ctgcgtgccg
     2461 gcgccgattt cgacggcctt gacggcattg atgcccatca gcgcgccggc gatgtcggcg
     2521 tcgagccggt cgaacaccgg ctcgccgaga cccggcggca cgccgctcgc gaccacagtg
     2581 acgcgcgcac cgatcgagtc gccctcccgg cgcagcgccc agaccagcgc ctcgagttcg
     2641 gccacgcgcg cggggtccgg gcagaagaac ggattccggt acgcgaacgc cggatcgacg
     2701 ggggcgagtt cgagcgcacc gatgcggctc aggtagccgt ggatccgtac gccgaggcgc
     2761 acggcgaggt acttgcgcgc gatcgcgccg gccgcgacgc gcatgaccgt ctcgcgcgcc
     2821 gaggagcgcc cgccgccgcg gtaatcgcgc acgccgtact tctgccagta ggtgtaatcg
     2881 gcgtggccgg ggcggaaacg gtcc
//
LOCUS       DAOQVY010000183         2891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_40543_length_2891_cov_4.117772, whole genome shotgun sequence.
ACCESSION   DAOQVY010000183 DAOQVY010000000
VERSION     DAOQVY010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40543_length_2891_cov_4.117772"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..1188)
                     /locus_tag="PKN91_12045"
     CDS             complement(154..1188)
                     /locus_tag="PKN91_12045"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28840.1"
                     /translation="MKKLRILLLVQESLVPPADLAGLSESAIDACRTEYNVLSTLTNL
                     GHDVSVLGIGDQIGELRRALRDTRPDIVFNLLEEFSGIVTYDHYVVAYLELMRQPYTG
                     CNPRGLMISRDKVLTKQVLAYHRIQTPHFHLFPWKARYREPRRPQFPLIVKSTTEDAS
                     LGISQASVVHDPAHLRERVQFIHEQAQSDAIAEQYIEGRELYIGVLGNDRLKTFPVWE
                     LDFGTLSDLQAGIATRKVKWDPDYQRKHGIRTGVALELPAGLETRLAALAKRIYRALH
                     MSGYGRMDLRLSPDGSVHVLEANANPNLTYGEDFAESADLAGLDYDGLISRIVSLGLS
                     YQPEWRLFES"
     gene            complement(1185..2195)
                     /locus_tag="PKN91_12050"
     CDS             complement(1185..2195)
                     /locus_tag="PKN91_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28841.1"
                     /translation="MYRLWRRLDDEQLLDLRFCDLPLDIRRGQVARQLSRLYAELRAR
                     GIRFRPHAWYGTEWFSPDGVPGFAVPFYLADPRLKRLERHVMQQVEGGDAKWLRRILR
                     HEAAHALDTAYGLRHRRDWRTVFGPASERYPSDYTSRPGSRRYVLHLGHWYAQSHPTE
                     DFAETFAVWLQPRARWRRDYAGWPALRKLEYVDRLMKEIAQRPPRRRNRRLVEPIAKN
                     RQTLRAHYRRKLGRYELSDWRYDRQLVRVFARPHRRARAAPAARFLREVRPQLERLLV
                     RRARLHPYVVAHALDTVTQRAQQLDLRLARDRRRSKRDAARLTERVVLDFLERNRENY
                     AL"
     gene            complement(2363..>2891)
                     /locus_tag="PKN91_12055"
     CDS             complement(2363..>2891)
                     /locus_tag="PKN91_12055"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNS28842.1"
                     /translation="DLLDRFLGAGNGRWVALFVIVSGLGALNGWTLIVGTLTATMAQN
                     GDFPARFAPLNRRNAPSRGLVLTGVLASTMILMNYSRSLVEGFVFLSTIVTAASLPMY
                     LCASLALVALWRRGERPASGDLAVAGLLGTTYVVIAFIGVGHEPFLWALALALAGVPV
                     GLWVRRGQRLAIASS"
BASE COUNT          477 a          940 c         1028 g          446 t
ORIGIN      
        1 tcgatgaagt cgccgaggcc caccgcctgt tcgagagccg ggcgttgcgc ggccgatcgc
       61 tggtccgcgt gggcggcgaa atctagttcg cagggtcatc gaggacgtgg accgatcggg
      121 cggtgatgtc gtcggcgcga tcagcctcct gcgctagctc tcgaacagcc gccattcggg
      181 ctggtagctg agcccgagtg acacgatccg gctgatcagc ccgtcgtagt cgagaccggc
      241 gagatcggcc gattccgcga aatcctcgcc ataggtcagg ttgggattcg cgttcgcctc
      301 gagcacgtgc acgctgccgt ccggactgag acgcagatcc atgcgcccgt aaccgctcat
      361 gtgcagtgcg cggtagatgc gtttggcgag cgcggcgagc cgcgtctcga gaccggccgg
      421 cagctcgagc gcgacgccgg tgcgtatgcc gtgcttgcgc tgatagtcgg ggtcccactt
      481 gaccttgcgt gtggcgatgc cggcctgcag atccgacagc gtgccgaagt cgagctccca
      541 gaccggaaag gtcttcagtc gatcattgcc gagcacgccg atgtagagct cgcgcccctc
      601 gatgtactgc tcggcgatcg cgtcgctctg cgcctgctcg tgaatgaact gcacgcgctc
      661 gcgcagatgc gccgggtcgt gcacgaccga ggcctgcgag atgccgagcg aggcatcttc
      721 ggtcgtcgat ttcacgatca gcggaaactg cggtcgccgc ggctcgcggt agcgcgcctt
      781 ccacggaaac aggtggaaat gcggtgtctg gatgcggtga taggcgagca cctgcttggt
      841 cagcaccttg tcgcgcgaga tcatcaggcc gcgcggattg cagccggtgt agggctggcg
      901 catcagctcg agatacgcga cgacgtagtg gtcgtaggtg acgatgcccg agaattcctc
      961 gagcaggttg aagacgatgt cggggcgggt gtcacgcagc gcgcggcgca gttcgccgat
     1021 ctggtcgccg atgccgagca cgctcacgtc gtgaccgagg ttggtgagcg tcgacagcac
     1081 gttgtactcg gtgcggcagg cgtcgatcgc gctttccgac agtccggcga gatcggccgg
     1141 cggcaccagg ctctcctgca cgagcagcag tatcctgagt ttcttcacag cgcgtagttc
     1201 tcgcggttgc gctcgaggaa gtcgagcacc acccgttcgg tgagccgcgc cgcatcgcgc
     1261 ttgctgcgcc ggcggtcgcg cgcgaggcgc aaatcgagct gctgcgcccg ctgggtcacg
     1321 gtgtcgagcg catgcgcaac cacatacgga tgcaggcgcg cgcggcgcac cagcagccgc
     1381 tcgagctgcg ggcgcacctc ccgcaggaag cgcgcggcgg gcgcggcgcg cgcgcggcga
     1441 tgcggtcgcg cgaacacgcg cacgagttgc cggtcgtagc gccagtcgga aagctcgtag
     1501 cgcccgagct tgcggcgata atgtgcgcgc agagtctgcc ggtttttcgc gatcggctcg
     1561 accagccggc gattgcgccg gcgcggcggc cgctgcgcga tctccttcat caggcgatcg
     1621 acatactcga gcttcctgag cgcgggccag cccgcgtagt cgcggcgcca gcgcgcgcgc
     1681 ggctgcagcc agacggcaaa tgtttcggcg aaatcctcgg tcgggtggct ctgcgcatac
     1741 cagtgaccga gatgcaggac atagcggcgg ctcccggggc gcgacgtgta atcggatggg
     1801 tagcgctccg acgccgggcc gaacacggtg cgccagtcac gccggtggcg caggccgtag
     1861 gcggtatcga gggcgtgcgc ggcttcgtgc cgcagtatgc gccgtagcca cttcgcgtcg
     1921 ccgccttcga cctgctgcat gacgtggcgt tcgagacgct tcaggcgcgg atcggcgaga
     1981 tagaacggca ccgcgaaccc gggcacgcca tcgggcgaaa accactcggt gccgtaccag
     2041 gcatgcggcc ggaagcggat gccgcgtgca cgcagttccg cgtacagccg gctcagttgc
     2101 cgtgcgacct ggccgcggcg gatgtcgagc ggcagatcgc agaagcgcag gtcgagcagc
     2161 tgctcgtcgt cgaggcggcg ccagagcctg tacatcagcg gaccatcgct cgtgcgaatg
     2221 ggcgggattc gggcgagccg attccggtcg gcaacgacat tcgactcaac acctccccgg
     2281 cgtgaaaagc gatagttgga gcataccgga tatccgtgac accactgcgt gcacgctcgc
     2341 ctggcgcggg ctcctggccg cgctacgagg aggcgatcgc gagtcgctga ccgcggcgca
     2401 cccacagccc caccggcacg ccggcgagcg cgagggcgag cgcccagagg aacggctcgt
     2461 ggccgacgcc gatgaaggcg atgaccacgt aggtggtgcc gagcaggccg gccacggcga
     2521 gatcgcccga cgcgggccgc tcgccgcgcc gccacagggc caccagcgcg agcgaagcgc
     2581 acaggtacat cggcagactc gctgccgtga cgatggtgct caggaacacg aagccctcga
     2641 ccagcgagcg gctgtagttc atcaggatca tcgtgctcgc gagcacgccg gtcagcacga
     2701 gcccgcgcga gggcgcgttg cggcgattga gtggcgcaaa gcgcgccggg aagtcgccat
     2761 tctgtgccat cgtcgcggtg agcgtgccga cgatcagcgt ccaaccgttc agcgcgccga
     2821 gaccgctcac gatcacgaac agtgcgaccc agcggccgtt acccgcgccg aggaagcggt
     2881 ccagcaggtc g
//
LOCUS       DAOQVY010000184         2825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_41989_length_2825_cov_5.049458, whole genome shotgun sequence.
ACCESSION   DAOQVY010000184 DAOQVY010000000
VERSION     DAOQVY010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41989_length_2825_cov_5.049458"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PKN91_12060"
     CDS             complement(<1..652)
                     /locus_tag="PKN91_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28843.1"
                     /translation="MGAHKKPSEPHHDPDLDSTAELPSLDDPLSSTDTYAIPTALVAD
                     ASGGESADPLEAEIGALRSDLATATARRGALELDLRSLSANLRDVEERLIRKGERLVT
                     IEQELEAARAACAEAVELGAREASERERANEELAAARSKAEELGREVESRALAAAALE
                     ARGAELAGEADRLRGEMRALRETLAERDIQLAERANSLAARERRLAEIEPLAAQRAQ"
     gene            753..1916
                     /locus_tag="PKN91_12065"
     CDS             753..1916
                     /locus_tag="PKN91_12065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS28844.1"
                     /translation="MRNVWILTLAQAMAACGMLVMFAFGGIVGARIAPTAALATLPLS
                     LGVVGVAAASIPAALSMQRFGRRPMFVLSALVAALAGLGCAWAVAHAHFGAFCAGALV
                     LGANMAFVQQYRFAAAEYVDVPLVPRAIAAVLLGTLAAAFLGPEAGDRLRNIGGWPEF
                     TGSFIAVAVFLVLSALTLLALGPPMAHALARHDGGRPLRQIVRQRAYLVALFAGLTSY
                     AVMSFIMTATPISMHVIDGHDVTATKNVITSHLVAMYLPSLASGWLIARLGIARMMMA
                     GIVCMLACIVIAAALGHAFLHYFSGLVLLGVGWNLLFVAATTLLTRTYSPAERFRAQG
                     FNDFATFTSQAIASLAAGAAISSIGWLNVNLVCIPLLLAMLVAVSAWRRQVSR"
     gene            complement(1906..2427)
                     /locus_tag="PKN91_12070"
     CDS             complement(1906..2427)
                     /locus_tag="PKN91_12070"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNS28845.1"
                     /translation="MRRAYFECRYGQLHVHNAMPAGGGFDEATPLLCIHRSGATGRMF
                     AGLMRLVGRDRSVYAPDLPGCGESDGPRAPASWAEQAAALGDFLADLRLRRVDLLAAG
                     AGAFVAAELALAQVAIVRRVAFLDPVDAASDREARERLPLLTQPTLLLHAPDDLDAHL
                     PAVTAELRTFLSD"
     gene            2575..2736
                     /locus_tag="PKN91_12075"
     CDS             2575..2736
                     /locus_tag="PKN91_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28846.1"
                     /translation="MKLYGYVGSPYVVRPLLLARWWQTVQADPVAAALRSGFHGNFRA
                     FLAARMKAA"
BASE COUNT          352 a         1026 c          987 g          460 t
ORIGIN      
        1 gctgcgcacg ctgcgcggcg agcggctcga tctcggcgag ccggcgctcg cgcgccgcga
       61 gactgttggc acgctcggcg agctggatgt cgcgctcggc cagcgtttcg cgcagcgcac
      121 gcatttcgcc gcgcaagcgg tcggcttcgc ccgcgagctc cgcgccacgc gcctcgagcg
      181 ccgccgcggc gagcgcccgt gattcgactt cgcggcccag ttcctcggcc ttcgagcggg
      241 ccgcggcgag ttcttcgttc gcacgctcgc gctcgctcgc ctcgcgcgcg ccaagctcga
      301 cggcctcggc gcaagcggcg cgcgccgctt cgagttcctg ctcgatcgtc acgagccgct
      361 cgcccttgcg gatcagccgt tcttcgacgt cgcgcaggtt cgcggacagg gaacgcaggt
      421 cgagctcgag tgcgccgcgg cgcgcggtgg cggtcgcaag atccgagcgt agtgcgccga
      481 tctcggcttc gagcgggtct gcgctctcgc cgccggacgc gtcggccacg agcgccgtcg
      541 ggatcgcgta ggtgtcggtg gacgacaggg gatcgtcgag cgacggcagc tcggccgtac
      601 tgtcgaggtc ggggtcgtga tgcggctcgg agggcttctt gtgcgcaccc atggccccga
      661 caagtctagc gcggcgcgcg gtgtgcggcg cgtggcgtcg cacaatggga cataaccccg
      721 gtaacatggc cgccccgcga aggaagcacc ccgtgcgcaa cgtctggata ctcacgctgg
      781 cgcaggccat ggccgcctgc ggcatgctcg tcatgttcgc cttcggcggc atcgtcggcg
      841 cccgaatcgc ccccaccgcc gccctcgcca ccctgccgct ctccctcggg gtcgtcgggg
      901 tcgcggccgc ctcgatcccc gcggcgctgt cgatgcagcg cttcgggcgc cggccgatgt
      961 tcgtcctgag tgcgctcgtc gcggccctgg cggggctcgg ttgcgcctgg gcggtcgccc
     1021 acgcccattt cggcgcgttc tgcgccgggg cccttgtgct gggggccaac atggccttcg
     1081 tgcagcaata ccgtttcgcg gcggccgagt atgtcgacgt gccgctcgtg ccgcgggcga
     1141 tcgccgcggt gctgctcggg accctggcgg cggcgttcct cgggcccgag gccggcgacc
     1201 gcctgcgcaa catcggcggc tggccggagt tcaccggctc gttcatcgcc gtcgcggtgt
     1261 tcctcgtgct gtcggcactg accctgctcg cgctcgggcc gccgatggcg cacgcgctcg
     1321 cgcgccacga cggcggacgg ccgctgcgcc agatcgtgcg ccagcgcgcc tacctcgtcg
     1381 cactgttcgc cgggctcact tcctacgccg tgatgagctt catcatgacg gcgacgccga
     1441 tcagcatgca cgtgatcgat ggccacgacg tcaccgccac gaagaacgtg atcacctcgc
     1501 acctggtcgc gatgtacctg ccatcgctcg cgagcggctg gctcatcgca cggctcggca
     1561 tcgcccgcat gatgatggcg ggcattgtct gcatgctcgc atgcatcgtg atcgccgccg
     1621 ccctcgggca cgcgttcctg cactatttca gcggactcgt gctgctcggc gtcggctgga
     1681 acctgctgtt cgtggcggct acgacgctgc tgaccaggac ctactcgccc gccgagcggt
     1741 tccgtgcgca gggattcaat gatttcgcga ctttcacgtc gcaggcgatc gcatcgctcg
     1801 ccgccggagc tgcgatcagc tcaatcggct ggctcaacgt gaacctggtg tgtatcccgc
     1861 tgctgctcgc gatgctggtc gcggtgagcg cctggcggcg gcaagtcagt cgctgaggaa
     1921 ggtgcgcagc tcggcggtga ccgccggcag gtgcgcatcg aggtcgtcgg gcgcatgcag
     1981 cagcagcgtc ggttgggtca gcagcggcag gcgctcgcgc gcctcccggt ccgacgccgc
     2041 atccaccggg tcgaggaagg cgacgcgacg cacgatggca acctgcgcca gcgccagttc
     2101 ggccgcaacg aacgcccccg cgccggccgc gagcagatcg acacgccgca gccgcaggtc
     2161 ggcgaggaag tcacccagtg ccgcggcctg ctccgcccag ctcgccggcg cgcgaggccc
     2221 gtcggattcg ccgcagcccg gcaggtccgg cgcgtacacc gagcggtcgc ggccgacgag
     2281 gcgcatcagg cccgcgaaca tgcgcccggt cgcgccgctg cgatggatgc aaagcagtgg
     2341 cgtcgcctcg tcgaatccgc cgccggctgg catcgcgttg tgtacgtgca gctgtccgta
     2401 gcggcactcg aagtaggcgc ggcgcacgtc cggctccgtg tcacgtacca caacctttcc
     2461 gcgccttgcc atgatggact gtccgctcct cgcacggccc gatgctgccg cgggcgccaa
     2521 ctatagcgac aatcccgtcc ctgccctgtg accgctgccc caataaggtc caccgtgaaa
     2581 ctctacggct atgtcggatc gccttatgtc gtgcgcccgc tcctgctggc gcgctggtgg
     2641 cagaccgtgc aggcggaccc ggtcgccgcg gcgctgcgca gcgggttcca cgggaatttc
     2701 cgcgccttcc tggcagcgag gatgaaggcg gcctgaggcc gcgctcaggg agcgcgcacg
     2761 gcgccgatct cggtgagccg cgcgatcgcg cgcgcctggc gctcgcggaa gtagtcgcga
     2821 tcggg
//
LOCUS       DAOQVY010000185         2819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_42111_length_2819_cov_7.028220, whole genome shotgun sequence.
ACCESSION   DAOQVY010000185 DAOQVY010000000
VERSION     DAOQVY010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42111_length_2819_cov_7.028220"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..571
                     /locus_tag="PKN91_12080"
     CDS             140..571
                     /locus_tag="PKN91_12080"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNS28847.1"
                     /translation="MESPPLFVHCCHCRWCQRETGSAFALNAMVEADRVTLLAGTPLL
                     VETPSLSGKGQRIARCPACQVALWSHYAGAGPAISFVRVGTLDEPDRVPPDIHIYTST
                     KQPWVVIPEGALAVTEFYDPRQTWPAASLARFKAARARHSA"
     gene            596..1510
                     /locus_tag="PKN91_12085"
     CDS             596..1510
                     /locus_tag="PKN91_12085"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNS28848.1"
                     /translation="MPAAAAAFRTRMIVSFAIVYIVWGSSYLFTSLGVHRLPPFLFAG
                     IRFVIAGVALTAIAHARGERIRLDATDARQLLVMALLSVAVSNGANVWAMQWVPSNQT
                     ALLNVTSSFWIAIFGLYGARGHPIDARTAIGLGLGFAGTLAIVWPREGFVTTNVLPQL
                     AILTGCVGWAAGTIYFRNARKSLDILAFTGLQMFVGGVILTAIGLGLGEHREWTWSPI
                     GLMAMFYLTVFSSCLAYTAYAWLAQHTSPARVATYGYVNPALATLLGWWVLGEHLTGI
                     ELAGMAVILAGVVLVNWPEEVATPESPG"
     gene            complement(1513..1881)
                     /locus_tag="PKN91_12090"
     CDS             complement(1513..1881)
                     /locus_tag="PKN91_12090"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNS28849.1"
                     /translation="MTGFILRAVIAAFGLWLASAWVDGFSITTTPTLLIAAALLGVVN
                     ATIRPIAIVLTFPITIVTLGIFLLVINAGMLGLVAWALPDFSIAGFWSALLGAIIVSI
                     VSWIGTWFVGSSLKVDAIRR"
     gene            complement(1878..2324)
                     /locus_tag="PKN91_12095"
     CDS             complement(1878..2324)
                     /locus_tag="PKN91_12095"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNS28850.1"
                     /translation="MAGKLRGYAHRIDINADTSRVWRALTEPAALARWCAPGATIKGR
                     PGGSFRASVDRIGVIDAHIDVFDAGRRLRLIHMEQDDLPAQGVTLIDDFLLEADGATT
                     IVRLLGSGYPDDKIWDPYFLRLRTSWERALARLKVYLDKNLDKEAK"
     gene            2385..2768
                     /locus_tag="PKN91_12100"
     CDS             2385..2768
                     /locus_tag="PKN91_12100"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HNS28851.1"
                     /translation="MAWVHAVIALALIEYLYFGAAVARARGRYKVPAPATTGHEAFER
                     CYRVQANTLESLIVLIPSMLIFALYIDPLWSAALGFVFVLGRVGYFVGYTRAARKRHW
                     GYMASILPMIALTGGALIGALRAAL"
BASE COUNT          429 a          965 c          937 g          488 t
ORIGIN      
        1 gtcgcgcggg ccaagcgcca atccggcgtc tgcgcctcag ctcaaacgtt aggcatcacg
       61 aacgcaactc actgccatgg ctgcagcaac ttcattcccg accgaaggcg gctgtgattg
      121 ccgcgccgta cgttaccgaa tggaaagccc gccgctgttc gtacactgct gccactgccg
      181 ctggtgccag cgtgagacgg gttccgcctt tgcgctgaac gcgatggtcg aggccgatcg
      241 cgtcacgctg ctcgccggca cgccgctgct ggtcgagacg ccctcgctca gcggcaaggg
      301 acagcgcatc gcacgctgtc ccgcctgcca ggtcgcgctc tggagccact atgcgggcgc
      361 gggtcccgcg atcagctttg tgcgcgtcgg tacgctcgac gagccggacc gcgtgccgcc
      421 cgacatccac atctacacct cgacgaagca gccctgggtg gtgatccccg agggcgcgct
      481 cgcggtgacg gaattctacg atccgaggca gacgtggccg gccgcgagtc tcgcgcgctt
      541 caaggccgcg agagcgcggc acagcgcctg acactccggc aggatcgccg cgcccatgcc
      601 cgccgccgcc gctgctttcc gcactcgcat gatcgtgtcc ttcgcgatcg tgtacatcgt
      661 gtggggatcg agctatctgt tcacctcgct cggcgtgcat cgcctgccgc cgttcctgtt
      721 cgccggcatt cgcttcgtca ttgccggcgt cgcgctcacc gcgatcgccc acgcgcgcgg
      781 tgagcgcatc aggctcgacg ccaccgacgc gcgccagctg ctcgtcatgg cgctgctctc
      841 cgtcgccgtg tcgaacggcg ccaacgtctg ggcgatgcaa tgggtgccct cgaaccagac
      901 tgcgttgctc aatgtcacct cttcgttctg gatcgcgatc ttcggactct atggcgcacg
      961 cggacacccg atcgatgcgc gcacggcgat cggcctcggg ctcggcttcg ccggcacgct
     1021 ggcgatcgtc tggccgcgcg agggcttcgt caccacgaac gtgctgccgc aactcgcaat
     1081 cctgacgggc tgcgtcggct gggcggcggg cacgatctac ttccgcaatg cgcgcaagtc
     1141 gctcgacatc ctcgcgttca ccggcctgca gatgttcgtc ggcggcgtga tcctcaccgc
     1201 gatcgggctg ggactcggcg agcatcgcga atggacctgg tcgccgatcg gcctgatggc
     1261 catgttctac ctgacggtgt tcagctcctg cctcgcctac acggcctacg cctggctcgc
     1321 gcaacacacg tcgccggcgc gggtggcgac ctacggctac gtgaatcctg cgctcgcgac
     1381 gctgctcggc tggtgggtgc tgggcgagca cctcaccggc atcgagcttg cgggcatggc
     1441 ggtgatcctg gccggagtgg tactggtcaa ctggcccgag gaagtcgcga cgcccgaatc
     1501 acccggctga cgtcagcggc ggatcgcgtc gaccttgagg ctcgagccga cgaaccacgt
     1561 gccgatccac gacacgatgc tgacgatgat cgcgcccagc agcgcagacc agaaaccggc
     1621 gatcgagaag tccggcagcg cccaggccac gaggcccagc atccccgcat tgatcaccag
     1681 cagaaatatg ccgagcgtca cgatggtgat cgggaaggtg aggacgatcg caatggggcg
     1741 gatggtcgcg ttcacgacgc cgagcagtgc ggcggcgatc agcagcgtcg gcgtggtcgt
     1801 gatggagaag ccgtcgaccc aggccgaggc cagccacagt ccaaaggccg cgatcacggc
     1861 ccgcaggatg aaacctgtca tttcgcctcc ttgtcgaggt tcttgtcgag atacaccttg
     1921 agccgcgcca gcgcgcgctc ccagctcgtg cgcaggcgca ggaagtaggg gtcccagatc
     1981 ttgtcgtccg gatagcccga gccgagcagc cggacgatcg tcgtggcgcc gtcggcctcg
     2041 agcaggaagt cgtcgatcag cgtgacgccc tgcgccggca ggtcgtcctg ttccatgtgg
     2101 atcaggcgca ggcggcgccc ggcatcgaac acgtcgatat gcgcatcgat cacgccgatg
     2161 cgatcgacgc ttgcccggaa gctgccgccc gggcgcccct tgatggtcgc gccgggcgcg
     2221 caccagcgcg cgagcgccgc cggctcggtg agcgcccgcc atacccggga cgtgtcggcg
     2281 ttgatgtcga tgcggtgtgc gtagccgcgc agcttgcctg ccatgggcgc tattctgcca
     2341 ccgccggttg cggctgggcg catggatcgc ggagaatcgc tcccatggca tgggtacacg
     2401 cagtgatcgc gctcgcactg atcgagtatc tgtacttcgg cgcggcggtc gcgcgcgcgc
     2461 gtgggcgcta caaggtgccg gcgcccgcca ccaccggcca cgaagcgttc gagcgctgct
     2521 accgcgtgca ggccaacacg ctcgaatcgc tgatcgtgct gattccctcg atgctgattt
     2581 tcgcgctgta tatcgacccg ctctggtcgg cggcactcgg cttcgtgttc gtgctcggcc
     2641 gcgtgggcta tttcgtcggc tacacgcgcg cggcacggaa gcgccactgg ggctacatgg
     2701 cctcgatcct gccgatgatc gcgctcaccg gcggcgcgct catcggggcg ttgcgcgcgg
     2761 cgctctagga gtctgggcgg cagaacctac tgacgagtgg agtgaggtga ggtagtttg
//
LOCUS       DAOQVY010000186         2788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_42826_length_2788_cov_5.380534, whole genome shotgun sequence.
ACCESSION   DAOQVY010000186 DAOQVY010000000
VERSION     DAOQVY010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42826_length_2788_cov_5.380534"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1794)
                     /locus_tag="PKN91_12105"
     CDS             complement(<1..1794)
                     /locus_tag="PKN91_12105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNS28852.1"
                     /translation="MSTPAFDAAPYAQIVRSLFARTRRVMLYGPDGRLWWSEDGADAL
                     DLRTLLEQVIALMRQRDLDAALRHAATAELSTGLLLPIRNDGPAPLAWLGIIGPETRA
                     AADPDATAIAQSLRPVLTLLARDVAAAALLRRQRYASLQAAEQTAELRWLLATASQAG
                     RNNGADRLEATLDACATHLGCDGVMLWLEDRQLERHALRRPLPPGELETLRRLARGSL
                     MSEVRTRKRTTIVAKLRETPDADLLPWTVIAAPVPHGDAPRGLIMAFARRDARRLSTR
                     DSRRLEHIIPRVLAATGMEHDPLTGLPARHVFEDKLRAALARSGSHACVVYADLDQLH
                     VTNDLFGFAAGDDVLRRMATLWREHTSERVEITARFAGDRFVALLRDCTLNQGRAWAE
                     TMRQAIEALPAPPERTGLRVTASFGVVSAAAGEAPEHVLATAETACKAAKDRGRNRVE
                     IFAQTDKSLVQRRDDLQIFRDLSDAFEQNRFMLYAQPIEPLWDPTRARRYEILVRMRD
                     GADDPVSPSRFISAATRYQLLPQLDRWVTARAIASLDPYADALRERNTTFAINVSAQS
                     LIQPEFADYVRTALKGAAIRADLLVFEFTESA"
     gene            complement(1900..>2788)
                     /locus_tag="PKN91_12110"
     CDS             complement(1900..>2788)
                     /locus_tag="PKN91_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNS28853.1"
                     /translation="ALFSFVQACIDSRRPALVAMPNPFYQIYEGATLLAGASPWFLNT
                     TAATGYLPDLDAVPASVWDDVQLLVLCSPGNPTGAVMSADYLARALVLAERHDFVIAA
                     DECYADLWFDEAQPPPSLLGAALAAGRDRYERCVVFHSLSKRSSVPGLRSGFVAGDPR
                     LMQAFLQYRTYHGCAMPVPTQHASIAAWNDDAHAAANRALYREKFELTVPILAPVLDL
                     TVPAGGFYLWPDVRGDDVRFVRELFATQNVTILPGSYLARDAHGENPGAGRVRISLVP
                     DAATCVEAARRIREFARKR"
BASE COUNT          430 a          924 c         1040 g          394 t
ORIGIN      
        1 cgcgctctcg gtgaactcga acaccagcag gtccgcgcgt attgcggcgc ctttcagcgc
       61 ggtgcgcacg tagtcggcga actcgggctg gatcagcgac tgcgcggaca cgttgatcgc
      121 gaaagtcgtg ttgcgctcac gcagcgcatc ggcgtagggg tcgaggctcg cgatggcgcg
      181 cgccgtgacc cagcggtcga gttgcggcag cagctggtag cgggtggcgg cggaaatgaa
      241 gcgcgatggc gagaccggat cgtcggcgcc gtcgcgcatg cgcacgagga tctcgtagcg
      301 gcgcgcccgc gtcggatccc acaacggttc gatcggctgc gcgtacagca tgaagcggtt
      361 ctgctcgaag gcgtcggaca ggtcgcggaa gatctgcagg tcgtcgcggc gctgcacgag
      421 gctcttgtcg gtctgcgcaa agatctcgac gcgattcctg ccgcggtcct tggccgcctt
      481 gcaggcggtt tcggccgtcg cgagcacgtg ctcgggcgcc tcgcccgcgg cggcggacac
      541 gacgccgaag ctcgcggtga ccctgaggcc ggtgcgttcc ggcggagcgg gcagggcctc
      601 gatcgcctgg cgcatggtct cggcccaggc gcgcccctgg ttcagcgtgc agtcgcgcag
      661 cagcgcgacg aagcggtcgc cggcaaagcg cgcggtgatc tcgacgcgct cgctcgtatg
      721 ctcgcgccag agcgtcgcca tgcggcgcag cacgtcgtcg cccgccgcga agccgaacag
      781 gtcgttcgtg acgtgcagct ggtcgagatc tgcatagacc acgcaggcgt gcgaaccgct
      841 ccgcgcgagc gctgcacgca gtttgtcctc gaagacgtgg cgcgcgggca ggccggtcag
      901 cgggtcgtgc tccatcccgg tggccgcgag cacgcgcgga atgatgtgtt cgaggcgtcg
      961 cgagtcgcgc gtcgacaggc gccgtgcgtc gcgccgggcg aacgccatga tcaggccccg
     1021 gggcgcatcg ccatgaggca cgggagccgc gatcacggtc cagggcagca ggtccgcatc
     1081 cggcgtctcg cgcaacttgg cgacgatcgt ggtgcgtttg cgggtcctga cttcgctcat
     1141 cagcgagccg cgcgcgaggc gccgcagcgt ctcgagttcc ccgggtggca acggacggcg
     1201 cagcgcatgg cgctcgagtt gccggtcctc gagccacagc atcacgccgt cgcagccgag
     1261 gtgggtggcg caggcatcga gcgtggcctc gagccggtcg gcgccgttgt tgcgcccggc
     1321 ctggctcgcg gtcgcgagca gccagcgaag ctccgccgtc tgctcggccg cctgcaggct
     1381 ggcgtagcgc tgcctgcgca gcagcgctgc ggccgccacg tcacgggcga ggagcgtcaa
     1441 taccgggcgc agcgattgcg cgatggccgt ggcatcgggg tcggcagccg cgcgcgtctc
     1501 ggggccgatg atgccgagcc aggccagggg cgcggggcca tcgttgcgga tcggcagcag
     1561 cagtccggtg gacagttcgg cggtggcggc atgccgcagc gccgcgtcga gatcacgctg
     1621 gcgcatcagt gcgatgacct gctcgagcag cgtgcgcagg tcgagcgcat cggcgccgtc
     1681 ctcgctccac cacaggcgcc cgtcgggccc gtagagcatc acgcgccgcg tgcgcgcaaa
     1741 cagcgagcgc acgatctgtg catacggtgc ggcatcaaac gcaggcgtcg acaccccaga
     1801 ctcccatgcc gggcgaatta tgacaaagct attgtcggcc aaccgccgga cgggcggcga
     1861 gacagccgtc acggatcgat gccgcggaat gtgcgtgggc tagcgcttgc gggcgaattc
     1921 ccggatccgg cgggccgcct cgacgcaggt ggcggcatcc ggcaccagcg aaatgcgcac
     1981 ccggccggcg ccggggttct cgccgtgggc gtcgcgggcg aggtaactgc ccggcaggat
     2041 ggtgacattc tgcgtggcga acaactcgcg cacgaagcgg acatcgtcgc cgcgcacgtc
     2101 cggccacagg tagaagccgc ccgctggcac cgtgaggtcg agcacgggcg cgaggatcgg
     2161 tacggtgagc tcgaacttct cgcggtacag ggcgcggttc gccgcggcgt gcgcgtcgtc
     2221 gttccaggcc gcgatgctcg catgctgggt cggcaccggc atcgcgcaac cgtgataggt
     2281 gcggtattgc aggaaggcct gcatcagccg cgggtcgccc gcgacgaaac cggaacgcag
     2341 cccgggcacg ctcgagcgct tcgagaggct gtggaacacg acgcaacgct cgtagcggtc
     2401 gcgacccgcg gcgagtgcag cgccgagcag ggacggtggc ggctgcgcct cgtcgaacca
     2461 caggtcggca tagcattcgt cggccgcaat cacgaaatca tggcgctccg cgagcacgag
     2521 cgcgcgggcg aggtagtcgg ccgacatcac cgcgccggtc ggattcccgg gcgagcagag
     2581 cacgagcagc tgcacgtcgt cccacacact cgccggtacg gcgtcgagat cgggcaggta
     2641 gccggtggcc gcagtggtgt tcagaaacca gggcgaggcg ccggccagca gcgtcgcacc
     2701 ctcgtagatc tggtagaagg gattcggcat cgcgaccagt gccggccgcc ggctatcgat
     2761 gcaggcctgc acgaaggaga acagtgcc
//
LOCUS       DAOQVY010000187         2786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_42874_length_2786_cov_5.596485, whole genome shotgun sequence.
ACCESSION   DAOQVY010000187 DAOQVY010000000
VERSION     DAOQVY010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42874_length_2786_cov_5.596485"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PKN91_12115"
     CDS             complement(<1..270)
                     /locus_tag="PKN91_12115"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNS28854.1"
                     /translation="MRVRIIAVGTRMPAWVTAGCDEYLKRLRPALRVELVEIPAGRRP
                     AGAPPTRAVEEEARKLLAALGADEHVVALDERGQEFSTLELARWLG"
     gene            complement(288..770)
                     /gene="rsfS"
                     /locus_tag="PKN91_12120"
     CDS             complement(288..770)
                     /gene="rsfS"
                     /locus_tag="PKN91_12120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNS28855.1"
                     /translation="MITARRSRPAASRATPLERLVTTALDDMKAVNVKVLDVRGLTDI
                     ADTFVIASGNSDRHVRSIADRVIQSAKAAGFPTHGLEGARDSEWVLVDLNDVIVHVML
                     PRTREFYGLERLWEGEADSAPAARPKRQARAKTKSKSGSAPAARPKARARAKSAARTH
                     "
     gene            complement(795..1439)
                     /gene="nadD"
                     /locus_tag="PKN91_12125"
     CDS             complement(795..1439)
                     /gene="nadD"
                     /locus_tag="PKN91_12125"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNS28856.1"
                     /translation="MQPIGIFGGTFDPIHYGHLRTAFELWQALRLKEVRFMPTGDPPH
                     RDAPLADAALRLAMVRVAIADQPAFVVDDRETRREGLSYSVDTLAELRREYPDRSLCL
                     LLGMDAFLGLPTWHRWRELPDLAHIVVAHRPGWKAPTTGPLGELMVDRGTGSVRDLHS
                     ATAGRIYVHAVTQLEISSTELRDLIVDGRDPRYLVPDAVRDMLRESGCYARNSR"
     gene            complement(1439..2374)
                     /gene="holA"
                     /locus_tag="PKN91_12130"
     CDS             complement(1439..2374)
                     /gene="holA"
                     /locus_tag="PKN91_12130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNS28857.1"
                     /translation="MKLSPDALGGHLARGLAPAYLLCGDEPLLVGEAADAVRARARAA
                     GFTDRVVHFIERGSSWADVRAEADSLSLFASRRILELRMPTGKPGASGSATIVDLVER
                     QDPDTLLLILTERLDRDAQGASWVRAIDAHGVVLTAWPIGRDKLPAWLEARARRAGLQ
                     LEPEALALLADRTEGNLLAARQEIDRLALGTRHGRVGVAELTASVGDSARFDVFALGA
                     AARAGNAARTLRIVAGLRAEGVEAPLVLWSLLREARQLLRDGGRFTRRDAPRLIGRAR
                     RVDRAIKGRLQSDAWDELALLAAELCGRTPLTGSY"
     gene            2433..2735
                     /locus_tag="PKN91_12135"
     CDS             2433..2735
                     /locus_tag="PKN91_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28858.1"
                     /translation="MRRIARHPLLLLLLAACMLGAAGVRAAHLHDAAQTDRHEVRQCD
                     LCHGFNTGAGNPAPLATTQPTLQPLGLVAPVPELGIAPQPFAQAHRPRGPPAPVTR"
BASE COUNT          406 a         1007 c          967 g          406 t
ORIGIN      
        1 accgagccag cgcgcgagtt cgagcgtcga gaattcctgg ccgcgctcgt cgagcgcgac
       61 gacatgctca tcggcgccaa gggccgcgag gagcttgcgc gcttcctctt ccactgcacg
      121 cgtcgggggc gcgccggccg gacgccgacc cgccgggatc tcgacgagtt cgacccgcag
      181 cgccggccgc aggcgcttga ggtattcgtc gcagcctgcg gtgacccacg ccggcatgcg
      241 ggtgccgacg gcgatgatac gcacccgcat ccggaccgcc cgccgattca gtgcgtgcgg
      301 gcggcggact tcgcccgcgc gcgggccttc ggacgggcgg cgggtgcgga gccggacttc
      361 gatttcgttt tcgccctcgc ctgccgcttg ggccttgcgg cgggcgcgct gtcagcctcg
      421 ccttcccaca ggcgctcgag cccgtagaac tcgcgcgtgc gcggcagcat cacatgcacg
      481 atcacgtcgt tcaggtcgac gagcacccat tcgctgtcgc gcgccccttc gaggccgtgg
      541 gtgggaaagc ccgcggcctt tgcgctctgg atgacccggt cggcgatgga tcggacgtgg
      601 cggtcggaat ttcccgacgc gatgacgaag gtatcggcga tgtcagtgag tccgcggacg
      661 tcgaggactt tcacattcac tgccttcatg tcgtcgagcg cagtggtgac gagtctctcg
      721 agcggcgtcg cgcgcgatgc ggcaggacgc gagcgtctgg cagtgatcat gtgcctgcga
      781 tggcctcccc tgtgtcagcg gctgttgcga gcatagcacc ccgattcgcg cagcatgtca
      841 cgcaccgcgt cagggacgag ataacgcggg tcccggccgt cgacgatcag gtcgcgcaac
      901 tctgtcgagg agatctcgag ttgcgtgacg gcgtggacgt agatgcgccc ggccgtggca
      961 ctgtgcaggt cgcgcaccga gccggtgccg cgatcgacca tcagctcgcc gagcggaccg
     1021 gtggtcggcg ccttccagcc cgggcggtgc gcgacgacga tgtgggcgag atcgggcagt
     1081 tcgcgccagc gatgccaggt cggcaggccg aggaaggcat ccatgccaag cagcaggcac
     1141 agcgagcggt ccgggtactc ccggcgcagt tcggcgagcg tgtcgaccga ataggacagg
     1201 ccctcgcgcc gggtctcgcg atcgtcgacg acaaacgccg gctggtccgc gatggcgacg
     1261 cgcaccatcg ccaggcgcag cgcggcgtcc gcgagcggcg catcacgatg cggcggatcg
     1321 ccggtcggca tgaagcgcac ctccttcagc ctgagcgcct gccagagctc gaacgcggtg
     1381 cgcaggtggc cgtaatggat cggatcgaag gtgccgccga atatgccaat gggttgcatc
     1441 aatagcttcc ggtcagtggt gtgcgtccgc agagttccgc cgcgaggagc gcaagctcgt
     1501 cccaggcatc cgactgcagc cggcccttga tcgcgcgatc gacccgcctt gcgcgcccga
     1561 tcaggcgcgg cgcgtcgcga cgcgtgaagc gcccgccgtc acgcagcagc tgccgcgcct
     1621 cgcgcagcag cgaccagagc acgagtggcg cctcgacgcc ttcggcgcgc aacccggcga
     1681 cgattcgcag cgtgcgcgcg gcattgcccg cgcgcgcggc ggcgccgagc gcgaacacgt
     1741 cgaagcgcgc gctgtcgccg acgctggcgg tgagctcggc gacgcccacc ctgccatggc
     1801 gcgtgccaag cgcgagccgg tcgatctcct gccgggccgc aagcaggttg ccctcggtcc
     1861 ggtcggccag cagggcgagc gcctcgggct cgagctgcag gcccgcgcgc cgcgcgcgcg
     1921 cctcgagcca ggccggcagc ttgtcgcgcc cgatcggcca ggcggtcagc accacgccgt
     1981 gggcatcgat cgcgcggacc caactcgcgc cctgcgcatc gcggtcgagc cgctcggtga
     2041 gtatcagcaa cagggtgtcg ggatcctgcc gctcgacgag atcgacgatg gtcgcactgc
     2101 cgctcgcacc gggcttgccc gtcggcatgc gcagttcgag gatccgccgc gaggcgaaca
     2161 gcgacaggct gtccgcctcg gcgcgcacat ccgcccagct gctgccgcgc tcgatgaaat
     2221 gcacgacgcg atcggtgaat cccgctgcac gcgccctggc gcgcacggca tccgccgcct
     2281 cgcccaccag cagcggctca tcgccgcaca gcaggtaggc cggtgcgagg ccgcgtgcga
     2341 gatgcccgcc cagggcgtcg ggggacaact tcattcagga atccgtgtca cgctccgatt
     2401 atccgccaga tcgggctaag atagccgccg aaatgcgccg catcgcccgc catccgctac
     2461 tgctgctgct gcttgccgca tgcatgctcg gcgcggctgg cgtgcgggca gcgcacctgc
     2521 acgatgcggc gcagacggac cgtcacgagg tccgccagtg cgacctgtgc catggattca
     2581 atacgggcgc cggcaatccc gccccgctcg cgaccacgca gccgacgctg cagcccctcg
     2641 gcctcgtggc gcccgtgccc gaactcggca tcgcgccgca gccgttcgcc caggcccacc
     2701 gacctcgcgg cccgcctgct cctgtaacgc gctgacactc gcccgccccg tcgcgggtgc
     2761 gctcgttcgc cttcaggagc cctcat
//
LOCUS       DAOQVY010000188         2771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_43212_length_2771_cov_5.647275, whole genome shotgun sequence.
ACCESSION   DAOQVY010000188 DAOQVY010000000
VERSION     DAOQVY010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43212_length_2771_cov_5.647275"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PKN91_12140"
     CDS             <1..349
                     /locus_tag="PKN91_12140"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNS28859.1"
                     /translation="LRFRDRCAALGIGVPIVPGLLPITRFPQVLRFAERCGATIPARL
                     RERFAGLDDDPDTRRLIAASVAIEQVERLREHGVVDFHFYTLNRAELTYAICHALGVR
                     PPAAPAIAVGATA"
     gene            346..>2771
                     /gene="metH"
                     /locus_tag="PKN91_12145"
     CDS             346..>2771
                     /gene="metH"
                     /locus_tag="PKN91_12145"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNS28860.1"
                     /translation="MSVCDTAHGHAPHGHGAGAIRGPGRSDVQAATAPYVIEPVDEAA
                     RTARIRKLRELLETRIVFLDGAMGTMVQRHKPDESAYRGARFADYGRDLRGNNEVLSL
                     TQPAMIEGIHRAYLEAGADIIETNTFSANAVSQSDYGMAALVPELNYASARLARRVAD
                     EVATASGETRFVAGAIGPTTRTASLSPDVSDPGFRNVTFDELAATYADSARALALGGV
                     DLLLVETVIDTLNAKAALFGARTALDELGIDLPIIVSGTITDASGRTLSGQTGEAFWN
                     SIRHAAPLAVGLNCALGAQQLRPYVEELSRVSDAYLCVYPNAGLPNAFGEYDEAPEQT
                     AAIVREFATAGFINILGGCCGTTPGHIRLIREAVAPVPRRVRPTIEPQCRLSGLEPLA
                     IGESSLFVNVGERTNVTGSAKFRKLIEAGDYAAAVEVARQQVTSGAQVIDVNMDEGML
                     DSEAAMVRFLNLIAAEPDIARVPVMIDSSKWAVIEAGLKCLQGKGIVNSISMKEGEGP
                     FLEQARRIRRYGAAVVVMAFDEQGQADTAARKVAICERAYRLLTEQVGFPPEDIIFDP
                     NIFAIATGIEEHDGYGLAFIEATREIKRRLPHALVSGGVSNVSFSFRGNDPVREAMHS
                     VFLYHAIQAGMDMGIVNAGQLAIYDDLDAELRTACEDVVLNRRADATERLLELAQRYK
                     GGRTERREADLAWRQLPVAKRLEHALVKGIDDFVVQDAEEARLSVDHPIKVIEGPLMD
                     GMNVVGDLFGAGKMFLPQVVKSARVMKKAVAHLVPYIELEKDGSSRANGKIVLATVKG
                     DVHDIGKNIVG"
BASE COUNT          449 a          954 c          952 g          416 t
ORIGIN      
        1 cctgcgcttc cgggaccgct gcgcggcgct cggcatcggc gtacccatcg tgccgggcct
       61 cctgccgatc acgcgcttcc cgcaggtcct gcgcttcgcc gagcgctgcg gcgcgacgat
      121 ccccgcgcgc ctgcgcgagc gtttcgccgg cctcgacgac gatcccgata cgcgacggct
      181 gatcgcggcg agcgtcgcga ttgagcaggt cgagcggctg cgcgagcacg gagtcgtcga
      241 tttccacttc tacacgctga accgcgccga actgacctac gcgatctgcc atgccctcgg
      301 cgtgcggccg cccgccgcgc cggcgatcgc cgtcggagcc acggcatgag tgtctgcgat
      361 acggcgcacg gccacgcccc tcacggccac ggcgcgggcg cgattcgcgg accgggccgc
      421 tccgacgtgc aggccgcgac cgcaccctat gtgatcgagc cggtggatga ggcagcgcgc
      481 actgcgcgca tccgcaagct gcgcgagctg ctcgagaccc gcatcgtgtt cctcgacggc
      541 gcgatgggca cgatggtgca gcggcacaag cccgacgagt cggcctaccg tggtgcgcgc
      601 ttcgccgact acggccgcga cctgcgcggc aacaatgaag tgctttcgct gacgcagccg
      661 gcgatgatcg agggcatcca ccgcgcctac ctcgaggcgg gcgccgacat catcgagacc
      721 aacaccttca gcgccaacgc cgtgtcgcag tccgattacg gcatggcggc gctcgtaccc
      781 gagctgaact acgcctcggc ccgcctcgcg cggcgcgtcg ccgacgaagt cgccacggcg
      841 agtggcgaga cgcgcttcgt cgccggcgcg atcgggccga cgacccgtac ggcttcgctg
      901 tcgccggacg tcagcgaccc gggctttcgc aatgtcacct tcgacgagct cgccgcgacc
      961 tatgccgatt cggcgcgcgc cctcgcgctt ggcggcgtgg acctgctgct ggtcgagacg
     1021 gtcatcgaca cgctgaacgc aaaggcggcg ttgttcggcg cacgcacggc actcgacgaa
     1081 ctcggcatcg acctgccgat catcgtctcg ggcaccatca cggacgcttc gggccgcacg
     1141 ctctcgggcc agaccggcga ggcattctgg aattcgatac gccacgccgc gccgctcgcc
     1201 gtcggcctca actgcgcgct cggtgcgcag cagctgcggc cgtacgtcga ggaattgtcg
     1261 cgggtgtcgg atgcatacct gtgcgtctat ccgaacgcgg gactgccgaa cgcgttcggc
     1321 gagtatgacg aggcgccgga gcagacggcc gcaatcgtgc gggagttcgc gaccgcggga
     1381 ttcatcaaca tcctgggcgg ctgctgcggc acgacacccg ggcacatccg cctgattcgc
     1441 gaggccgtgg ccccggtgcc gcgccgcgtg cgccccacga tcgagccgca gtgccggctc
     1501 tccgggctcg agccgctcgc gatcggcgag tcgagcctgt tcgtcaacgt cggtgaacgc
     1561 accaacgtca ccggcagcgc gaagttccgc aagctgatcg aggccggcga ctatgccgcg
     1621 gcggtcgaag tggcccgcca gcaggtgacg agcggcgcgc aggtcatcga cgtcaacatg
     1681 gacgagggca tgctcgactc ggaagcggcg atggtgcgct tcctgaacct gatcgcggcc
     1741 gagccggaca tcgcgcgcgt tccggtgatg atcgactcgt cgaagtgggc ggtgatcgag
     1801 gcaggcctca aatgcctgca gggcaagggc atcgtcaatt cgatcagcat gaaggagggc
     1861 gaggggccgt tcctcgagca ggcacgccgg atccgccgct acggcgccgc cgtcgtcgtg
     1921 atggcgttcg acgagcaggg ccaggccgac acggccgcgc gcaaggtcgc gatctgcgag
     1981 cgcgcctacc ggctgctcac cgaacaggtc ggctttccgc ccgaggacat catcttcgat
     2041 ccgaacatct tcgcgatcgc gaccggcatc gaggagcacg acggctacgg tctcgcgttc
     2101 atcgaggcga cgcgggaaat caagcggcgg ctgccgcatg cactggtgtc gggcggcgtc
     2161 agcaacgtgt cgttctcgtt ccgcggcaat gacccggtgc gcgaagcgat gcattcggtg
     2221 ttcctgtacc acgcgatcca ggcgggcatg gacatgggca tcgtcaatgc cggccagctc
     2281 gcgatctacg atgatctcga cgcggagttg cgcaccgcct gcgaggacgt ggtgctgaac
     2341 cgccgcgccg acgccaccga gcgcctgctc gaactcgcgc agcgctacaa gggcggcagg
     2401 accgagcggc gcgaggccga cctcgcctgg cggcagctgc cggtcgccaa gcgcctcgag
     2461 catgcgctcg tcaagggcat cgacgacttt gtggtgcagg acgccgaaga ggcgcgcctc
     2521 tcggtggatc acccgatcaa ggtgatcgaa gggccgctga tggacggcat gaatgtcgtc
     2581 ggcgacctgt tcggtgcggg caagatgttc ctgccccagg tggtcaagag cgcgcgcgtg
     2641 atgaagaagg cggtcgctca cctcgtgccc tacatcgagc tcgagaagga cggcagttcg
     2701 cgcgccaacg gcaagatcgt gctcgcgacg gtcaagggcg acgtgcacga catcggcaag
     2761 aacatcgtcg g
//
LOCUS       DAOQVY010000189         2765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_43336_length_2765_cov_5.969004, whole genome shotgun sequence.
ACCESSION   DAOQVY010000189 DAOQVY010000000
VERSION     DAOQVY010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43336_length_2765_cov_5.969004"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /gene="mtgA"
                     /locus_tag="PKN91_12150"
     CDS             <1..640
                     /gene="mtgA"
                     /locus_tag="PKN91_12150"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNS28861.1"
                     /translation="LRWVDPPGSMMMLEARIEAWRADDARYRHRYHWVDMAQVSPYAA
                     RAVIAAEDQNFAMHPGFDFEAIRKAAEHNADGRAVRGASTISQQVAKNLFLWRGRSWL
                     RKGLEAWFTVLIETLWPKARILEVYLNIAEFGHGIYGVEAAAQAYWKKSAKALTRSQA
                     ATLAAVLPNPRRYRAQPPSPYVQRRRDWILGQMPYVEMPAATGSATRAREAR"
     gene            complement(783..1235)
                     /locus_tag="PKN91_12155"
     CDS             complement(783..1235)
                     /locus_tag="PKN91_12155"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNS28862.1"
                     /translation="MRATPAGWPRISVSVFYEDPARAIDWLVDAFGFEVRLRIEGEGG
                     AIEHSELTYGEGLLFVGGAGAANRHPERAFPASPRSVGDRNTQAMCIFVDDVDAHCAA
                     ARKAGARIEMEPTTTDYGEEYWTDRCYLAVDPEGHRWWFMQRLRSGGQ"
     gene            complement(1314..>2765)
                     /locus_tag="PKN91_12160"
     CDS             complement(1314..>2765)
                     /locus_tag="PKN91_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNS28863.1"
                     /translation="VKGDVHDIGKNIVGVVLQCNNFEVVDLGVMVPCEQILEAARREQ
                     ADMIGLSGLITPSLDEMVHVAREMKRQGMNLPLLIGGATTSPAHTSVKIAPQYDEAVV
                     YVKDASRAVSVCQSLITADLRDEFVGKIAIEHERRREQHAGKRVKQPDVSIEAARANR
                     HPIDWRTFHPVRPASPGVHVFNDYPLEELLGYIDWMPFFNAWEFAGKFPDILTDPVVG
                     EAASNLYADARRMLKQIVAERWLTVNAVIGLWPANAVGDDIEIYADESRRSVLATLSH
                     LRQQKQKPKGHAHSCLSDYIAPRDTGVGDYIGGFAVTAGVGIEPHLERFRAAHDDYSG
                     IILKALADRLAEAAAEHFHERVRRELWAYAPEEQLTNDELIREGYRGIRPAPGYPACP
                     DHTEKRKLWRLLDAQQRAGITLTESYAMYPTAAVSGWYFSHPASHYFAVGKIARDQVL
                     DYARRKGWSIEEAERWLAPNLGYAPGENLEPAA"
BASE COUNT          441 a          945 c          918 g          461 t
ORIGIN      
        1 gctgcgctgg gtcgacccgc cgggcagcat gatgatgctc gaggcccgca tcgaggcgtg
       61 gcgagccgac gatgcgcgct accgccatcg ctaccactgg gtcgacatgg cgcaggtctc
      121 gccgtacgcg gcgcgtgcgg tgatcgcagc ggaagaccag aacttcgcca tgcatccggg
      181 cttcgatttc gaggcgatcc gcaaggccgc cgagcacaac gcggacgggc gcgcagtgcg
      241 cggcgcgagc acgatcagcc agcaggtcgc gaagaacctg ttcctgtggc gcgggcgctc
      301 gtggctgcgc aagggcctcg aggcctggtt caccgtgctg atcgagacgc tgtggcccaa
      361 ggcgcgcatt ctcgaggtgt acctcaacat cgccgagttc ggacacggca tctacggcgt
      421 ggaggctgcg gcccaggcct actggaaaaa gagcgcgaag gcgctgacgc gctcgcaggc
      481 ggcgacgctc gccgcagtgc tgccgaatcc gcgccgctac cgtgcgcagc cgccctcgcc
      541 gtatgtccag cgccgccgcg actggatcct cgggcagatg ccgtatgtcg aaatgccggc
      601 cgctacgggc agcgcgacgc gcgcccgcga agcgcgctga gcaggctgca ccatcgagcg
      661 aagggttcgc gccaccactg gcagagccgc accagtgctt tgtcttcgat gtccgttgcc
      721 tcgacgatcc cgctgcgcct cgacccgcgc aggcgctggc tcacgcgctg cgcggaccac
      781 tgtcactgcc cgccgctcct caggcgctgc atgaaccacc agcgatggcc ttcggggtcg
      841 accgccagat agcagcggtc ggtccagtat tcttcgccgt agtcggtggt cgtcggctcc
      901 atctcgatcc ttgcgccggc cttgcgcgcc gccgcacagt gcgcatcgac atcgtcgacg
      961 aatatgcaca tcgcctgggt gttgcgatcg ccgacgctgc gcgggctcgc gggaaacgcg
     1021 cgttcgggat gacggttggc cgcaccggcg ccgccgacga acaggagtcc ctcaccgtaa
     1081 gtgagctcgg aatgctcgat cgccccgccc tcgccctcga tcctgagccg cacctcgaaa
     1141 ccgaaggcat cgaccagcca gtcgatcgcg cgggcgggat cctcatagaa cacggacacg
     1201 gaaatgcggg gccaacccgc cggcgtcgca cgcatattga gtcctccctg acctgagttc
     1261 caacgtgctg agcaactata cccgcacgcc gtcggcaggg aggcgccgcg accctaggcc
     1321 gcgggttcca ggttctcgcc aggcgcatac ccgagattcg gcgcgagcca gcgctcggcc
     1381 tcttctatcg accagccctt gcggcgtgcg tagtcgagaa cctggtcgcg cgcgatcttg
     1441 ccgaccgcga aatagtgcga cgccggatgc gagaaatacc agccactcac tgcggccgtc
     1501 ggatacatcg cgtacgactc ggtcagcgtg atgccggcac gctgctgggc gtcgagcagc
     1561 cgccacagct tgcgcttctc ggtatggtcg gggcatgcgg ggtagcccgg cgccgggcgg
     1621 atgccgcgat agccctcgcg gatcagctca tcgttggtga gctgctcctc gggagcgtaa
     1681 gcccacaatt cgcgtcgtac ccgctcgtgg aagtgctcgg ccgccgcctc ggcgaggcga
     1741 tcggcgagcg ccttcaggat gatgccggaa tagtcgtcgt gcgcggcgcg gaacctctcg
     1801 aggtgcggct cgatccccac tcctgccgtc accgcgaatc cgccgatgta gtcgccgaca
     1861 cccgtgtcgc gcggtgcgat gtaatcgctc aggcaggagt gcgcgtggcc cttgggcttc
     1921 tgcttttgct ggcgcaggtg cgacagcgtc gccagcacgc tgcggcgcga ctcgtcggca
     1981 tagatctcga tgtcgtcgcc cacggcgttc gcgggccaca ggccgatgac ggcgttcacg
     2041 gtgagccagc gctccgcgac gatctgcttg agcatgcgtc gtgcgtcggc atacaggttg
     2101 ctcgcggctt cgccgaccac cgggtcggtg aggatgtcgg ggaacttgcc ggcgaactcc
     2161 caggcattga agaacggcat ccagtcgatg tagccgagca actcctcgag cgggtagtcg
     2221 ttgaatacgt gcactccggg cgatgccggg cgcaccgggt ggaacgtgcg ccagtcgatc
     2281 ggatggcggt tggcacgcgc cgcctcgatc gacacgtccg gctgcttcac gcgcttgccg
     2341 gcatgctgtt cgcgccgccg ctcgtgctcg atcgcaatct tcccgacgaa ctcgtcacgc
     2401 aggtcggcgg tgatcagcga ctggcagacg ctgacggcgc gcgaggcatc cttcacgtac
     2461 acgaccgcct cgtcgtactg cggcgcaatc ttgacggagg tgtgcgccgg cgacgtggtc
     2521 gccccgccga tcagcagcgg cagattcata ccctgacgct tcatctcacg cgcgacgtgc
     2581 accatttcat cgagcgaggg cgtgatcagg cccgagagcc cgatcatgtc ggcctgttcg
     2641 cgccgcgctg cctcgaggat ctgctcgcaa ggcaccatca cgccgaggtc gacgacctcg
     2701 aagttgttgc actggagcac gacgccgacg atgttcttgc cgatgtcgtg cacgtcgccc
     2761 ttgac
//
LOCUS       DAOQVY010000190         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_44898_length_2701_cov_5.764928, whole genome shotgun sequence.
ACCESSION   DAOQVY010000190 DAOQVY010000000
VERSION     DAOQVY010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44898_length_2701_cov_5.764928"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PKN91_12165"
     CDS             complement(<1..265)
                     /locus_tag="PKN91_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650899.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNS28864.1"
                     /translation="MRELGVFSEIHPWDVTDEDVRRFRPKGIILSGGPESTTESAAPA
                     APAAVFELGVPVLGICYGMQTMAKQLGGRVASSDHREFGYAEVT"
     gene            complement(336..1802)
                     /gene="guaB"
                     /locus_tag="PKN91_12170"
     CDS             complement(336..1802)
                     /gene="guaB"
                     /locus_tag="PKN91_12170"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNS28865.1"
                     /translation="MRILEEALTFDDVLLVPAYSEVLPREVDLSTPLTRTIRLNLPLI
                     SAAMDTVTEARLAITMAQEGGIGVIHKSMSIEGQAREVARVKKFESGVIRDPITATPD
                     MTIRQVLDVTRAHGISGVPVVNGGKVVGIVTHRDLRFEEKLDAPVSSVMTPQERLITV
                     PENASKEDVLLLLHKHRIEKVLVVGDKFELRGMITVKDFQKAHEFPRACKDEGGRLRV
                     GAAVGTSPDTPDRVAALAEAHVDVVIVDTAHGHSRGVLQMVEKLKKAWPDLQVIGGNI
                     ATPAAAADLVAAGADAVKVGIGPGSICTTRIVAGVGVPQISAVANVAASLAKQGIPLI
                     ADGGIRYSGDVAKAIVAGAYAVMIGGLFAGTDESPGEVELYQGGSYKAYRGMGSLGAM
                     AERHGSSDRYFQDTTAELEKLVPEGIEGRVPYRGGLVAILHQLSGGLRAAMGYTGSSN
                     ITEMRTRPQFMRITNAGMRESHVHDVTITKEAPNYRIR"
     gene            1896..>2701
                     /gene="xseA"
                     /locus_tag="PKN91_12175"
     CDS             1896..>2701
                     /gene="xseA"
                     /locus_tag="PKN91_12175"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNS28866.1"
                     /translation="MAVERNVYTVSRLNREVRLLLERGLPGVWVEGEISNLARPSSGH
                     WYFSLKDRDAQIRCAMFRQKNSAVKFVPKDGAAVVARGRVSLYEPRGDFQLIVEHLEE
                     AGLGALQREFERLRDKLAAEGLFDEAGKRALPEVPRRIGVVTSPTGAALRDILHVLGR
                     RFAPVPVLIYPTPVQGAAAVPGILAALERAAQRQDCDVLILARGGGSLEDLWAFNDER
                     VARAIRAMPMPVVTGIGHEIDFTIADFVADLRAPTPSAAAQHTVPDGRAWL"
BASE COUNT          405 a          924 c          913 g          459 t
ORIGIN      
        1 aggtcacctc ggcgtagccg aattcgcgat gatcgctgct cgcgacccgt ccgccgagct
       61 gcttggccat cgtctgcatg ccgtagcaga taccgagcac gggtacgccg agttcgaaga
      121 ccgcggccgg cgctgccggc gcggcgctct cggtggtcga ttcggggccg ccggagagga
      181 tgatgccctt cgggcggaag cgcctgacat cctcgtcggt gacatcccag gggtggattt
      241 cactgaacac cccgagttcg cgcacccggc gggcgatgag ctgggtgtac tgcgcgccga
      301 agtcgaggat caggatgcga tcctcgtgca ggtccttatc tgatgcggta attgggcgct
      361 tccttggtaa tcgtcacgtc gtgcacgtgg ctctcgcgca tgcccgcatt ggtgatgcgc
      421 atgaactgcg ggcgcgtgcg catctcggtg atattgctgc tgccggtata gcccatcgcg
      481 gcgcgcaggc cgccggagag ctggtgcagg atggcgacga ggccgccccg gtagggcacg
      541 cgcccttcga tgccttccgg cacgagtttc tcgagctccg cggtcgtatc ctggaaatag
      601 cggtcgctcg agccgtggcg ttcggccatc gcgccgagcg agcccatacc gcggtaggcc
      661 ttgtaggaac caccctggta gagctcgact tcgcccggcg attcatcggt gcccgcgaac
      721 aggccgccga tcatcaccgc gtaggcgccg gcgacgatcg ccttggccac atccccgctg
      781 tagcggatgc cgccgtcggc gatcagcggt atgccctgct tcgcgaggct cgcggcgaca
      841 ttggcgacgg ccgagatctg cggcacgccg acgcccgcga cgatgcgcgt cgtgcagatc
      901 gagccgggcc ctataccgac cttgaccgca tcggcgccgg ccgcgaccag atcggcggcg
      961 gcggcgggtg tcgcgatatt gccgccgatc acctgcaggt cgggccacgc cttcttgagc
     1021 ttctccacca tctgcagcac gccgcgcgaa tggccgtgtg cggtgtcgac gatgaccaca
     1081 tcgacgtgcg cttccgcaag cgccgccacc cgatcgggcg tgtcgggtga agtcccgacc
     1141 gccgcaccga cgcgcaggcg cccgccctcg tccttgcagg cgcgcggaaa ctcgtgcgcc
     1201 ttctggaaat ccttgacggt gatcatgccg cgcagttcga acttgtcgcc gaccaccagc
     1261 accttctcga tgcgatgctt gtgcagcagc agcagcacgt cttccttcga ggcgttctcg
     1321 ggcaccgtga tgagccgctc ctgcggcgtc atcacgctcg acaccggcgc atcgagcttc
     1381 tcctcgaagc gcaggtcgcg gtgcgtgacg atacccacga ccttgccgcc gttgacgacc
     1441 ggaacacccg agatgccgtg cgcccgcgtg acgtcgagca cctggcggat cgtcatgtcc
     1501 ggcgtcgcgg tgatcggatc gcggatcacg ccgctctcga atttcttcac gcgggcgact
     1561 tcgcgcgcct gcccttcgat cgacatgctc ttgtggatga cgccgatgcc gccttcctgc
     1621 gccatcgtga tggcgaggcg cgcctcggtc accgtgtcca tcgcggcgga aatcagcggc
     1681 aggttcagtc ggatcgtgcg ggtcagcggt gtcgacaggt cgacttcgcg cggcaggact
     1741 tccgagtagg cgggtactaa caggacatcg tcgaaggtca gtgcttcttc gaggatacgc
     1801 attacggggg aatgtccaag ggaattggac gccgcattgt acgcatgcac cgctcgcccg
     1861 taaaggcgag cgcgcgccgc tacaatggcc gcgccatggc cgtcgaacgc aacgtctaca
     1921 ccgtcagccg cctcaaccgg gaagtgcgcc tgctgctgga acgggggctg ccgggcgtct
     1981 gggtcgaggg cgagatctcc aacctcgccc ggccctcctc cggccactgg tatttctcgc
     2041 tgaaggatcg cgacgcgcag atccgctgtg cgatgttccg gcagaagaac tcggccgtga
     2101 agttcgtacc gaaggacggc gcggccgtcg tggcgcgcgg gcgggtcagc ctgtatgagc
     2161 cgcgcggcga cttccagctg atcgtcgagc atctcgagga ggccggactc ggggcactgc
     2221 aacgcgagtt cgagcggctg cgcgacaagc tcgccgccga gggcctgttc gacgaggcgg
     2281 gcaagcgggc gctgcccgag gtgccgcggc gcatcggtgt ggtcacctcg ccgaccggag
     2341 cggcgctgcg cgacatcctg cacgtgctcg gacgtcgctt cgcaccggtc ccggtactca
     2401 tctacccgac ccccgtgcag ggtgcggcgg ccgtgcccgg catcctcgcc gcgcttgagc
     2461 gcgccgcgca acggcaggac tgcgacgtgc tgatcctcgc gcgcggcggc ggctcgctcg
     2521 aggatctgtg ggccttcaac gacgagcgcg tggcgcgcgc gatccgcgcg atgccgatgc
     2581 cggtggtgac cggcatcggc cacgaaatcg acttcacgat cgcggacttc gtcgccgacc
     2641 tgcgcgcacc gacaccctcg gccgccgccc agcacacggt ccccgacggg cgcgcctggc
     2701 t
//
LOCUS       DAOQVY010000191         2650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_46262_length_2650_cov_5.528709, whole genome shotgun sequence.
ACCESSION   DAOQVY010000191 DAOQVY010000000
VERSION     DAOQVY010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46262_length_2650_cov_5.528709"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PKN91_12180"
     CDS             complement(<1..456)
                     /locus_tag="PKN91_12180"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNS28867.1"
                     /translation="MPAAGAGRRYGAGVLRKQYEPLAGATVIEHALAPFLADARCYRI
                     VVAIAPGDAAWPQVAARCQARGGCPVGAVDGGAERADSVRRALGALQAGADDDQWVLV
                     HDAVRPCVSRAEIDALVDAVHAHPVGGLLAVPLADTLKRAAPSRGNELAR"
     gene            complement(496..816)
                     /gene="ftsB"
                     /locus_tag="PKN91_12185"
     CDS             complement(496..816)
                     /gene="ftsB"
                     /locus_tag="PKN91_12185"
                     /inference="COORDINATES: protein motif:HMM:NF002058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HNS28868.1"
                     /translation="MKWLAGALLIVVLLLQYRLFASPDGLREMRRLEADVAAQRGENE
                     ALVERNARLAAEVRDLKQGLTALEERARSELGMVAPRETFYQVVPRPGSATGAEPRTR
                     TAHR"
     gene            complement(839..2125)
                     /gene="eno"
                     /locus_tag="PKN91_12190"
     CDS             complement(839..2125)
                     /gene="eno"
                     /locus_tag="PKN91_12190"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNS28869.1"
                     /translation="MSRIVDIRGREIIDSRGNPTVEADVTLDSGVRGRAAVPSGASTG
                     TREAVELRDGDAGRFLGKGVLKAVEHVNGVLAAALKGRDPADQAGLDAAMIELDGTDT
                     KGRLGANALLAISLANAHAAALDAGKELYESLAGARAPVLPVPMMNIVNGGAHANNSL
                     DIQEFMILPVGAPSLAEALRCGAEVFQHLKKLLAARGLTTAVGDEGGFAPSLDSNAEA
                     LSMIAEAIDAAGYRLGSDVYLGLDVASSEFYRDGRYVLEGEGREFTPAEFADYLASLV
                     ARYPIITIEDGMAEGDWDGWAQLTRLLGSKVQLVGDDLFVTNTAILREGIAKGIANAI
                     LIKPNQIGTLTETLAAIDMAAAAKYAAIVSHRSGETEDTSIADIAVATAATQIKTGSM
                     SRSDRVAKYNRLLRIEAALGKRASYAGRQAFTVPVA"
     gene            complement(2122..>2650)
                     /gene="kdsA"
                     /locus_tag="PKN91_12195"
     CDS             complement(2122..>2650)
                     /gene="kdsA"
                     /locus_tag="PKN91_12195"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNS28870.1"
                     /translation="VVDVLQTPAFLVRQTDFIVNAASQGRPVNIKKGQFLSPWEMQNV
                     VDKARSTGNQEIMVCERGFTFGYNNLVSDMRSLAVMRDTGCPVVFDATHSVQLPGGKG
                     TASGGQREFVPVLARAAVGAGIAGLFMETHPDPDKALSDGPNAWPLGRMEALLTTLKA
                     LDGAVKSQPYEESLL"
BASE COUNT          393 a          932 c          916 g          409 t
ORIGIN      
        1 ccgcgcgagc tcgttgccgc ggctaggcgc ggcgcgcttg agcgtgtcgg cgagcggcac
       61 ggcgagcagc ccgccgaccg gatgcgcgtg caccgcgtcc accagcgcat cgatctcggc
      121 gcgcgacacg cagggccgca cggcgtcgtg caccaatacc cattggtcgt cgtcggcccc
      181 cgcctgcagc gcgccgagcg cgcgccgcac gctgtccgcg cgctctgcac caccgtccac
      241 ggcgccgacc ggacagccac cgcgcgcctg gcagcgagcc gcgacctgcg gccaggcggc
      301 gtctcccggg gcgatggcga cgacgatgcg gtagcagcgt gcatcggcca ggaatggcgc
      361 gagcgcatgc tcgatgacgg tggcaccggc gagcggctcg tactgcttgc gcagaacacc
      421 ggcgccatat cgccggccgg cgccagcggc cggcactacg agcgcatatc gcatcgggga
      481 gacgccgccg gccggctacc ggtgggcagt gcgggtacgc ggctccgcgc ccgtggcaga
      541 gcccggccgg ggcacgacct ggtagaaggt ttcgcgcggc gcgaccatgc cgagttcgct
      601 gcgggcccgc tcctcgagcg cggtgaggcc ctgcttgaga tcacggactt cggcggcgag
      661 gcgcgcattg cgctcgacaa gcgcttcgtt ctcgccgcgc tgggcggcga cgtcggcttc
      721 gaggcggcgc atttcgcgca gcccgtccgg cgaggcgaag aggcggtact gcagcagcag
      781 cactacgatc aacaacgctc ccgccaacca cttcatggcc gcgtgactct accagacctc
      841 atgccacggg gacagtgaat gcctggcggc cggcgtagct ggcgcgcttg ccgagcgcgg
      901 cctcgattcg cagcagccgg ttgtacttcg caacgcggtc cgaacgcgac atcgaaccgg
      961 tcttgatctg cgtggcggcg gtcgccaccg cgatgtccgc gatggacgtg tcctcggttt
     1021 cgcccgagcg gtgcgagacg atcgccgcgt acttcgccgc cgcggccatg tcgatcgccg
     1081 ccagcgtctc ggtcagcgtg ccgatctggt tcggcttgat caggatcgca ttggcgatgc
     1141 ccttggcgat gccctcgcgc aggatcgccg tgttggtgac gaacaggtcg tcgccgacca
     1201 gctgcacctt gctgccgagc aggcgcgtga gctgcgccca gccgtcccag tccccctccg
     1261 ccatgccgtc ctcgatcgtg atgatcgggt agcgggcgac gagcgaagcg aggtagtccg
     1321 cgaactcggc cggggtgaac tcgcggccct caccttcgag cacgtagcgg ccatcgcggt
     1381 agaactcgga actcgcgacg tcgaggccga ggtagacgtc actcccgagc cgatagcccg
     1441 cggcgtcgat cgcctcggcg atcatcgaga gcgcctcggc gttcgaatcg aggctcggcg
     1501 cgaagccgcc ctcgtcgccc accgcggtgg tcagtccacg cgccgcgagc agcttcttca
     1561 ggtgctggaa tacctcggcg ccgcagcgca atgcctcggc gaggctcggc gcaccgaccg
     1621 gcaggatcat gaactcctgg atgtcgaggc tgttgttggc atgcgcaccg ccgttcacga
     1681 tgttcatcat cggcacgggc agcaccggcg cgcgcgcgcc ggcgagcgat tcgtacagct
     1741 ccttgccggc atccagcgct gcggcgtgcg cgttggcgag cgaaatcgca agcagggcat
     1801 tcgccccgag cctgcccttc gtgtcggtgc cgtcgagttc gatcatcgcc gcatcgaggc
     1861 cggcctggtc ggccggatcg cgtcccttga gcgccgcggc gagcacgccg ttgacgtgtt
     1921 cgaccgcctt cagcacgcct ttgcccagga agcggccggc atcgccgtcg cgcagctcca
     1981 ccgcctcacg cgtgccggtc gatgcgccgg acggcacggc cgcacggccg cgcactccgc
     2041 tgtccagcgt gacatccgcc tcgacggtgg gattgccgcg tgaatcgatg atctcgcggc
     2101 cacggatgtc gacgatgcga ctcatagcag ggattcctcg tagggctgtg atttgacggc
     2161 gccatcgagc gccttgagtg tggtgaggag cgcttccatg cggccgagcg gccaggcatt
     2221 cggtccgtcg gaaagcgcct tgtcgggatc cggatgggtt tccatgaaca ggcccgcgat
     2281 gcccgcgccg acggccgcgc gtgcgagcac cggcacgaac tcgcgctggc cgcccgaggc
     2341 cgtacccttg ccgcccggca gctgcaccga atgcgtggcg tcgaacacga ccggacagcc
     2401 ggtgtcgcgc atcaccgcga gcgaacgcat gtcggacacc aggttgttgt agccgaaggt
     2461 gaagccgcgc tcgcagacca tgatctcctg attgcccgtc gaccgcgcct tgtcgacgac
     2521 gttctgcatc tcccagggcg agaggaactg gcccttcttg atgttgaccg gcctgccctg
     2581 cgaggcggca ttgacgatga aatcggtctg gcggacgagg aacgcgggcg tctgcagcac
     2641 gtcgaccacc
//
LOCUS       DAOQVY010000192         2618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_47188_length_2618_cov_5.099103, whole genome shotgun sequence.
ACCESSION   DAOQVY010000192 DAOQVY010000000
VERSION     DAOQVY010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47188_length_2618_cov_5.099103"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1036)
                     /gene="gcvT"
                     /locus_tag="PKN91_12200"
     CDS             complement(41..1036)
                     /gene="gcvT"
                     /locus_tag="PKN91_12200"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNS28871.1"
                     /translation="MGRHTPLFDLHVRLGARMVDFGGWDMPVHYGSQIEEHHAVRRNA
                     GVFDVSHMCVIDLTGEHVRDLLRSLLANDVEKLRIPGKALYSCLLNESGGVLDDLIVY
                     HFTEEFFRLIVNAGTRDKDLAWIRPRAAERGVAVAERIDLAILAVQGPASRTIVETLL
                     PREAEGRSAVMKLAPFSGRVHGKWFIARTGYTGEDGFEIVLPATEAEATWIALNERGV
                     ASCGLGARDTLRLEAGMNLYGNDMDESTNPLESGLGWTVAFEPEGREFIGRKALEAIR
                     DRGNLRRFVGLLLEDRGVLRSHQKVIVEGVGEGEITSGVFDASSALNFAASTPAG"
     regulatory      complement(1050..1154)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            1308..2537
                     /gene="ispG"
                     /locus_tag="PKN91_12205"
                     /gene_synonym="gcpE"
     CDS             1308..2537
                     /gene="ispG"
                     /locus_tag="PKN91_12205"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNS28872.1"
                     /translation="MSLLPRRTTLAVHVGPVVVGGAHPIVVQSMTNTDTADVEATAQQ
                     VKALARAGSELVRVTVNSAEAAAAVPEVRERLAQLGVDVPLIGDFHFNGHKLLREYPA
                     CAEALAKYRINPGNVGRGSKRDPQFAEMIETACRYDKPVRIGVNWGSLDQDLLTRMMD
                     ENSGAAEPRSAHAVMRDAIVTSAVASAARARELGLPAGRIVLSAKVSGVQDLIAVYRD
                     MATRCQYPLHLGLTEAGMGSKGIVASTSAMAVLLQEGIGDTIRVSLTPEPGGDRTQEV
                     IVAQEILQTMGLRAFIPMVVACPGCGRTTSSYFQQLAQSIQSHVRHRMPEWRKNYEGV
                     EDMTVAVMGCVVNGPGESKHASIGISLPGTGERPVAPVYEDGEKTVTLKGDNIAAEFR
                     ELVERYVERRFARRSPP"
BASE COUNT          445 a          900 c          866 g          407 t
ORIGIN      
        1 gcgtcatgac ctatagccgc ccttatcctt gggaaaaatc ctatcccgct ggcgtcgatg
       61 cggcgaagtt caaggcggag ctcgcgtcga agacgccgct cgtgatctcg ccctcgccga
      121 caccctcgac gatcaccttc tggtggctgc gcagcacgcc gcgatcctcg agcaggaggc
      181 cgacgaacct gcgcaggttg ccgcgatcgc ggatcgcctc gagcgccttg cggccgatga
      241 actcgcgccc ctccggttcg aacgcgacgg tccagccgag gcccgattcg agcgggttgg
      301 tcgattcatc catgtcgttg ccgtacaggt tcatgcccgc ctcgaggcgc agcgtgtcgc
      361 gcgcaccgag cccgcaggag gccacgccgc gctcgttgag cgctatccac gttgcctcgg
      421 cctcggtggc gggcagcacg atctcgaaac cgtcttcgcc cgtgtagccg gtgcgcgcaa
      481 tgaaccactt gccgtgcacg cgaccgctga atggcgcgag cttcatcacc gcggaacgtc
      541 cttcggcctc ccgtggcagc agagtctcga cgatggtgcg cgacgccgga ccctgcacgg
      601 cgaggatcgc gagatcgata cgctcggcga ctgcgacgcc gcgttccgcg gcgcgcggcc
      661 ggatccaggc gagatccttg tcgcgggtgc cggcgttgac gatgaggcgg aagaactctt
      721 cggtgaagtg gtagacgatc aggtcgtcga gcacgccacc cgactcgttc agcaggcagg
      781 agtagagcgc cttgcccggg atcctcagct tctcgacgtc gttcgcgagc agcgagcgca
      841 gcaggtcgcg cacgtgctca ccggtgagat cgatgacgca catatgtgac acgtcgaaca
      901 cgccggcatt gcggcgcacc gcatggtgct cctcgatctg cgaaccgtag tgaacgggca
      961 tgtcccagcc gccgaaatcg accattcggg cgccaaggcg tacatgcagg tcaaaaagcg
     1021 gtgtatgtcg tcccatgcgg agccacttcc accgcccctc tgtcctgtga cctgagagat
     1081 cgggcgggtc caccgggact cgcctcaccc cttcggtggg tgggcaggac ccaccactct
     1141 ccagagctcg cctcggacct gccgttcgtt tgcctgagcg tttccgggcg gaacttgcgc
     1201 cttcggcgtc cccgccccgg ggggcgcgga tctctccgga cagtcttgcg tggcggcgag
     1261 cgggcacatg ataccagcga attccccagc gactagaata atgtcccatg tcgctcctgc
     1321 cccgtcgcac caccctcgcc gtacacgtcg gacccgtcgt ggtcggcggc gcacacccga
     1381 tcgtcgtgca gtcgatgacc aacaccgata ccgcggatgt cgaagccacc gcgcaacagg
     1441 tgaaggcgct ggcgcgcgcc gggtccgagc tggtccgcgt caccgtgaac agcgccgagg
     1501 ccgccgccgc ggtccccgaa gtgcgcgagc ggctcgcgca gctgggtgtc gatgtgccgc
     1561 tcatcggcga tttccacttc aacggccaca agctgctgcg ggagtatccc gcgtgcgccg
     1621 aggcgctggc caagtaccgg atcaaccccg gcaacgtggg ccgcggcagc aaacgggatc
     1681 cgcagttcgc cgagatgatc gagacggcat gccgctacga caagccggta cgcatcggtg
     1741 tcaactgggg cagcctcgac caggacctgc tgacgcgcat gatggacgag aactccggcg
     1801 cggccgagcc gcgttccgcg cacgcggtga tgcgcgacgc catcgtcact tccgcggtgg
     1861 cgagtgccgc gcgcgcgcgc gagctcggcc tgcctgccgg gcgcatcgtg ctgtcggcga
     1921 aagtcagcgg cgtgcaggac ctgatcgccg tgtaccgcga catggcgaca cgctgccagt
     1981 atcccctgca cctcggcctc accgaggcgg gcatgggctc caagggcatc gtcgcctcga
     2041 cctcggccat ggcggtactg ctgcaggaag gcatcggcga cacgatccgc gtgtcgttga
     2101 ccccggagcc gggcggcgat cgcacgcagg aggtgatcgt cgcgcaggaa atcctgcaga
     2161 ccatgggcct gcgcgccttc atcccgatgg tggtcgcgtg cccgggctgc ggtcgcacga
     2221 ccagcagcta tttccagcag ctcgcgcaat cgatccagtc ccacgtacgc catcgcatgc
     2281 ccgaatggcg caagaactac gaaggcgtcg aggacatgac cgtcgcggtc atgggctgcg
     2341 tcgtcaacgg cccgggtgag agcaagcacg cgagcatcgg catcagcctg ccggggacgg
     2401 gcgagcggcc cgtggcgccg gtctacgaag acggcgagaa gaccgtgaca ctcaagggcg
     2461 acaacatcgc cgcggagttc cgcgaactgg tcgaacgcta cgtcgagcgc agattcgcgc
     2521 gccggagccc gccatgaccg acctcagccg gagaaaatgc gtgccatgcg aaggcggcat
     2581 tgcgccgctc gagcgcgccg acgccgagcg ttactgcg
//
LOCUS       DAOQVY010000193         2434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_52919_length_2434_cov_3.767129, whole genome shotgun sequence.
ACCESSION   DAOQVY010000193 DAOQVY010000000
VERSION     DAOQVY010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52919_length_2434_cov_3.767129"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PKN91_12210"
     CDS             <1..631
                     /locus_tag="PKN91_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNS28873.1"
                     /translation="VGKKVEELITRLAQKARASGIHLVLATQRPSVDVITGLIKANIP
                     CRIAFQVSAKVDSRTILDQGGAESLLGHGDMLYLQTGTSVPTRVHGAFVSDQEVLRVV
                     EALRRNAPPVYVDEVLSGPSTPIPGMPGEDGEGEGGGADAEADPLYDEAVKIVTTERK
                     PSISYVQRRLKIGYNRAARLLEAMESSGLVGPLQSNGAREVLAPAPPED"
     gene            631..1275
                     /gene="lolA"
                     /locus_tag="PKN91_12215"
     CDS             631..1275
                     /gene="lolA"
                     /locus_tag="PKN91_12215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HNS28874.1"
                     /translation="MYKAVLAAAGLALVTIGGAGAAATEPPTPIDRYLDGLRTLRAEF
                     TQRLVDARGVTVQEAQGSLVVQRPGRFRWEVRPAGAVGGQLLVADGRNLWFFDRDLEQ
                     VTVKPADASLTATPAMLLSGVGDIRAAFAIEPLPRSGGLDWVRVVPRANDADFRDARL
                     GFDGRELKRMELNDKLGQSATLLFSRTQRNVPVDPAELRFTPPPGADLIGTPEN"
     gene            1281..1679
                     /locus_tag="PKN91_12220"
     CDS             1281..1679
                     /locus_tag="PKN91_12220"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNS28875.1"
                     /translation="MQSLRYRSWWIVGGLAMVLAIVVTSLLPSSSLPSVQVWDKFEHA
                     VAYFVLAAWFGGIVRPDRYGRLALALLSLGIAVEVFQQAMGLGRTAELRDVLANAVGI
                     VLGLAIAVLGVSGWMRGVERMLGVAVPNPE"
     gene            1693..>2434
                     /locus_tag="PKN91_12225"
     CDS             1693..>2434
                     /locus_tag="PKN91_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS28876.1"
                     /translation="MSPPPPDRSRPLADRMRPRSLDEVVGQEHILGSGKPLRMALEGG
                     QLHSMILWGPPGTGKTTLARLVARRADAVFIALSAVLAGVKDVRAAVEQAKANRAQSG
                     RATVLFLDEVHRFNKAQQDTFLPFLEDGTLTFIGATTENPSFEVVSALLSRARVYVLK
                     PLAPDSIAQLLRAALADRERGLGADGLAIDDEALLLLSQAADGDARRALNMLEIAADL
                     AEGGRITLAAAREVASGGRRRFDKGGEQF"
BASE COUNT          355 a          849 c          852 g          378 t
ORIGIN      
        1 cgtcggcaag aaggtcgagg agctgatcac gcgcctcgcg cagaaggcgc gcgcctccgg
       61 catccacctc gtgctcgcca cccagcgccc ctcggtcgac gtgatcacgg gcctcatcaa
      121 ggcgaacatc ccgtgccgca tcgccttcca ggtctcggcc aaggtcgatt cgcgcacgat
      181 cctcgaccag ggcggcgccg aatcgctcct tggccatggc gacatgctct acctgcagac
      241 cggcacgtcg gtgccgaccc gcgtgcacgg cgccttcgta tccgaccaag aagtgctgcg
      301 cgtggtcgag gcactgcgac gcaatgcgcc cccggtctac gtcgacgaag tcctctcggg
      361 tcccagcacg ccgatcccgg gcatgcccgg cgaggacggc gagggcgagg gaggcggcgc
      421 ggatgccgag gccgatccgc tctacgacga ggccgtcaag atcgtcacca ccgagcgcaa
      481 gccatcgatc tcgtacgtac agcgccggct caagatcggc tacaaccgcg ctgcgcgcct
      541 gctcgaggcg atggagtcgt caggtctcgt cggtccgctg caatcgaacg gcgcgcgcga
      601 agtgctcgcg cccgcgccac cggaggattg atgtacaagg cagtccttgc ggcggcgggc
      661 ctcgcgctcg tgacgatcgg cggcgcaggc gcggcggcga ccgagccgcc gaccccgatc
      721 gaccgctacc tcgatggcct gcggacgctg cgtgcggagt tcacgcagcg actcgtcgat
      781 gcgcgcggcg tcaccgtgca ggaggcgcag gggagcctcg tcgtacagcg gcccgggcgc
      841 tttcgctggg aggtgcggcc agccggcgcg gtcggaggcc agttgctcgt ggccgacggc
      901 cgcaacctct ggttcttcga cagggacctg gagcaggtca cggtgaagcc ggccgacgcc
      961 tccctcaccg caacccccgc gatgctgcta tcgggtgtcg gtgacattcg cgcggcgttc
     1021 gcgatcgagc cgctgccgcg ttcgggcggc ctcgattggg tgcgcgtcgt gccgcgcgcc
     1081 aacgatgccg atttccgcga cgcgcggctc ggattcgacg gccgtgaact gaagcgcatg
     1141 gagctgaacg acaagctcgg gcagagtgcc acgctgctgt tcagcaggac gcagcgcaat
     1201 gtgcccgtgg acccggcgga actgcggttc acgcccccgc cgggcgccga tctcatcggc
     1261 acgccggaaa attgaccccg atgcagtcac tgcgctaccg gtcctggtgg atcgtcggcg
     1321 gtctggccat ggtgctcgcg atcgtcgtca cctcgctgtt gccctcgagc agcctgccga
     1381 gcgtccaggt gtgggacaag ttcgagcacg ccgtcgcgta cttcgtgctc gcggcctggt
     1441 ttggcggcat cgtgcgcccg gatcgctacg ggcgcctcgc gctcgcgctg ctctccctcg
     1501 gcattgcggt ggaagtcttc cagcaggcga tgggcctcgg gcggacggcc gagctgcgcg
     1561 atgtgctggc gaatgccgta ggcatcgtcc tcggacttgc catcgccgtg ttgggcgtga
     1621 gcggctggat gcgcggcgtg gagcgcatgc tgggcgtcgc cgtgccaaac cccgagtagg
     1681 cgcgcagcgc tcatgagccc gccgccgccc gatcgcagcc ggccactcgc cgatcgcatg
     1741 cgaccgcgtt cgctggacga ggtcgtcggc caggaacata tactcggcag cggcaagccg
     1801 ttgcgcatgg cgctcgaggg cggacagctg cactcgatga tcctgtgggg cccgccgggc
     1861 accggcaaga ccaccctcgc gcggctggtc gcgcgccgcg ccgatgccgt gttcatcgcc
     1921 ttgtccgcgg tgctcgccgg cgtgaaggac gttcgcgccg cggtggagca ggcgaaggcc
     1981 aatcgcgcgc agtccggccg cgcaaccgta cttttcctcg acgaagtgca tcgcttcaac
     2041 aaggcacagc aggatacatt cctgcctttc ctcgaggacg gcacgctcac cttcatcggc
     2101 gcgacgaccg agaacccgtc gttcgaagtc gtcagcgcgc tgctctcacg ggcgcgcgtg
     2161 tacgtgctga agccgctggc gcctgattcg atcgcgcaac tgctgcgcgc ggcattggcg
     2221 gaccgcgagc gcggcctcgg ggccgacggg ctcgcgatcg acgacgaggc tctgctgctc
     2281 ctctcgcagg cggccgacgg cgatgcgcgg cgcgcactca acatgctcga aatcgccgcg
     2341 gaccttgccg agggcgggcg tatcacgctc gccgctgccc gcgaggtggc gagcggcggg
     2401 cggcggcgct tcgacaaggg cggcgagcag ttct
//
LOCUS       DAOQVY010000194         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_58384_length_2289_cov_4.904208, whole genome shotgun sequence.
ACCESSION   DAOQVY010000194 DAOQVY010000000
VERSION     DAOQVY010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58384_length_2289_cov_4.904208"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PKN91_12230"
     CDS             complement(<1..694)
                     /locus_tag="PKN91_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019408857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNS28877.1"
                     /translation="MFKRLRGYFSSDLSIDLGTANTLIYVRDKGIVLNEPSVVAVQDE
                     PNRGGKVIVAVGADAKGMLGRTPGHITAVRPMKDGVIADFTYTEKMLQYFINKVHGNR
                     MLKPSPRVLVCVPIGSTQVERRAIKESAEGAGARSVGIVSEPMAAAVGAGLPVHEARG
                     SMVLDVGGGTSEVAVLSLNGIVYAASARVGGDRFDEAIMNYVRRNYGILIGEATAERI
                     KIEIGSAYPGQQV"
     gene            813..1103
                     /gene="gatC"
                     /locus_tag="PKN91_12235"
     CDS             813..1103
                     /gene="gatC"
                     /locus_tag="PKN91_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNS28878.1"
                     /translation="MSLNRSDIENIARLARLSLKEEEVAGYVQTLGNILEMVGQLDRA
                     DTAGVDPMAHPLAGQTQRLRADEVTETDRHALYQENAPRVEGGYYIVPRVIE"
     gene            1100..>2289
                     /gene="gatA"
                     /locus_tag="PKN91_12240"
     CDS             1100..>2289
                     /gene="gatA"
                     /locus_tag="PKN91_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNS28879.1"
                     /translation="MSDVPLHRRTLAELAAGLARRDFSSVELTRHLLARLEAAQPHLN
                     AMVSITAESALAAAAQADGVTAAGKAGPLTGLPIVHKDIFCTAGVRTTCGSRMLENFV
                     SPYDATVVARLKAAGTVMLGKSNMDEFAMGSSNETSYFGPVRNPWDKALVPGGSSGGS
                     AAVVAARLAPAATATDTGGSIRQPAALTGITGLKPTYGRVSRYGMIAFASSLDQAGVI
                     AASAEDCALLLTAMAGFDPRDSTSVDAPVPDYAAALAAPARGLRIGIVREFFDKGLDA
                     DNEREVRAALAVYESLGATIEEVSLPNVGLSVPTYYVVAPAECSSNLARFDGVRFGYR
                     CEQPKDLLDLYQRSRGEGFGAEVKRRIMTGTYVLSAGYYDAYYLKAQRVRQLISADFA
                     RAFER"
BASE COUNT          374 a          784 c          757 g          374 t
ORIGIN      
        1 gcacctgctg gcccgggtag gccgagccga tctcgatctt gatgcgctcg gcggtcgcct
       61 cgccgatcag gatgccgtag ttgcgacgca cgtagttcat gatcgcctca tcgaagcgat
      121 cgccgccgac gcgcgccgag gcggcgtaga cgatgccgtt cagcgacagc accgccactt
      181 ccgaagtgcc gccaccgaca tcgagcacca tggagccgcg cgcttcgtgc accggcaggc
      241 cggcaccgac cgcggcggcc atcggctcgc tgacgatccc gacgctgcgc gcgccggcgc
      301 cctcggcgga ttccttgatc gcgcgccgct cgacctgcgt ggagccgatc ggcacgcaca
      361 ccagtacgcg cggcgagggc ttcagcatcc ggttgccgtg taccttgttg atgaagtact
      421 gcagcatctt ttcggtgtag gtgaagtcgg caatgacgcc gtccttcatc ggacgcaccg
      481 cggtaatgtg cccgggcgtg cgcccgagca tgcccttggc gtcggcgccg accgcgacga
      541 tcaccttgcc gccgcggttg ggttcgtcct ggaccgcgac caccgagggc tcgttcagga
      601 cgataccctt gtcgcgcacg tagatgagcg tattggcagt gcccaggtcg atcgaaagat
      661 cgctggaaaa atagccgcga aggcgtttga acatgaaagc gaccggacct tcgggaaatc
      721 cccgtaaaag tggccggtaa tctagcaacc gacaacactt tccacaatac gaggtgtatg
      781 atttcgcgcc gtttttccgt gatcttcctc tcatgagcct caatcgatcg gacatcgaaa
      841 acatcgcccg cctcgcgcgc ctgtctctca aggaggagga agtggccggc tatgtccaga
      901 ccctcggaaa catcctcgaa atggtgggcc agctggatcg cgccgatacc gccggcgtcg
      961 atccgatggc gcatccgctt gccgggcaga cgcagcgact gcgtgccgac gaggtcaccg
     1021 agaccgaccg gcatgcgctg taccaggaga atgccccgcg ggtcgagggc ggctactaca
     1081 tcgtgccgcg ggtgatcgaa tgagcgacgt accgctgcac aggcgaacgc tcgccgaact
     1141 ggccgccggc ctcgccaggc gcgatttctc gagcgtcgag ctcacccgtc acctgctggc
     1201 gcgactcgag gcggcacagc cgcatctgaa cgccatggtg tcgatcacgg ccgagtcggc
     1261 gctcgccgcg gccgcccagg ccgacggcgt cactgccgcg ggcaaggccg ggccgctgac
     1321 cggcctgccg atcgtgcaca aggacatctt ctgcaccgcg ggcgtacgca cgacctgcgg
     1381 ctcgcgcatg ctcgagaact tcgtctcgcc ctacgacgcc accgtcgtcg cgaggctcaa
     1441 ggccgcgggc accgtgatgc tcggcaagtc gaacatggac gaattcgcga tgggctcgtc
     1501 gaacgagacg agttatttcg gtccggtgcg caacccgtgg gacaaggcgc tggtgccggg
     1561 cggctcctcg ggtggctccg cagccgtcgt cgctgcgcgc ctcgcgcctg ccgccacggc
     1621 caccgatacc ggcggctcga tccgccaacc cgccgcactg accggcatca ccggcctgaa
     1681 gccgacctac ggccgcgtgt cgcgctacgg catgatcgcc ttcgcatcga gcctcgacca
     1741 ggccggcgta atcgcggcgt cggccgagga ctgcgcgctg ctgctcacgg cgatggcggg
     1801 cttcgatccg cgcgactcga ccagcgtcga tgcgccggtg cccgactacg cggcggcgct
     1861 cgcggcgcct gcccgcgggc tgcgcatcgg catcgtcagg gagttcttcg acaaggggct
     1921 cgatgccgac aacgagcggg aggtgcgcgc ggcgctcgcc gtgtacgaat cgctcggggc
     1981 cacgatcgag gaagtgagcc tgccgaacgt cggcctgtcg gtgcccacct actacgtggt
     2041 ggcgccggcc gagtgttcgt cgaatctggc gcgcttcgac ggcgtgcgct tcggctatcg
     2101 ctgcgagcag ccgaaggacc tgctggacct ctaccagcgt tcgcggggcg agggcttcgg
     2161 cgccgaagtg aagcgccgca tcatgactgg cacctacgtg ctgtcggcgg gctattacga
     2221 cgcctattac ctgaaggcgc agcgcgtgcg gcagctgatc agcgccgatt tcgcgcgcgc
     2281 cttcgagcg
//
LOCUS       DAOQVY010000195         2179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_63141_length_2179_cov_5.661017, whole genome shotgun sequence.
ACCESSION   DAOQVY010000195 DAOQVY010000000
VERSION     DAOQVY010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63141_length_2179_cov_5.661017"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1559
                     /gene="uvrC"
                     /locus_tag="PKN91_12245"
     CDS             <1..1559
                     /gene="uvrC"
                     /locus_tag="PKN91_12245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNS28880.1"
                     /translation="RPRYNVLLRDDKSFPYILLRTNHEFPRFMSYRGPRHRDGRYFGP
                     FPSAAAVNQTLAQIQKLFRIRNCRDSFFAHRSRPCLQYQIGRCTAPCVGFITREDYAR
                     DVAAAVDLLDGRGGEVERQLTTRMEAAAATLDFEQAAQIRDQLAAVRELQSQQVVTAG
                     SDRDSDVFAIVGEPTEYAVSTMLVRGGRNLGTSTAFPGAGLAEPEEALSSFLMQYYAT
                     TEPPREIYVNLRLPDAEALATALGERASRRVGVSRPSRGLAARWVALTVENATQALRM
                     RRAQSEGVAEMRAALAAALDLPAPPERMECFDISHTGGEGTVASCVVFGPDGPLKKEY
                     RRFNITGIAPGDDYGALRQALTRRYTRVRSGEIPPPDVLFIDGGRGQVGEVRDVLAEL
                     GFADLTLVGVAKGADRRPGQERLFVYGEEAPRVLDPTSPALRLIQRIRDEAHRFAITG
                     HRKRRARRYNESVLEVVPGLGPARRRALLKHFGGLQGVLKAGVADLARAPGIGPALAQ
                     TLYDHLHPGA"
     gene            1559..2116
                     /gene="pgsA"
                     /locus_tag="PKN91_12250"
     CDS             1559..2116
                     /gene="pgsA"
                     /locus_tag="PKN91_12250"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNS28881.1"
                     /translation="MIWNLPNTLTWLRIFAIPLVVALYYLPFRWSDPAAGLLFAIAGI
                     TDSLDGYFARKYGQTSKLGAFLDPVADKLIVAVALVLIVSKDPRWFVVIIASVIIGRE
                     IAISALREWMAEIGQRKKVAVSRIGKHKTILQVTGLSMMLFRWDLFGLPIYDIGLVLT
                     VLAAVLTLWSMWLYLRAAWPEMRSG"
BASE COUNT          329 a          764 c          727 g          359 t
ORIGIN      
        1 atcggccgcg ctacaacgtg ctgctgcgcg acgacaagag cttcccgtac atcctgctgc
       61 gcacgaatca tgagtttccg cgattcatgt catatcgcgg accgcgacat cgcgacgggc
      121 gctatttcgg gccgtttccg agcgcggcgg cggtcaacca gacgctggcc cagatccaga
      181 agctcttccg catccgcaac tgtcgcgaca gcttcttcgc ccatcgcagc cgcccctgcc
      241 tgcagtacca gatcgggcgc tgcacggcgc cctgtgtcgg cttcatcacg cgcgaggact
      301 acgcgcgcga cgtggccgcc gccgtggatc tgctcgacgg tcggggaggc gaggtggagc
      361 gccagctcac gacgcgcatg gaggcggccg ccgcgacgct cgatttcgag caggcggcac
      421 agatccgcga ccagctggcc gcggtgcgcg aactgcagtc acagcaggtg gtcacggccg
      481 gcagcgaccg cgacagcgac gtgtttgcga tcgtcggtga accgaccgag tacgccgtga
      541 gcacgatgct cgtgcgcggc gggcgcaacc tcggcacgtc gaccgctttc cccggcgcgg
      601 ggctcgccga accggaagaa gcgctgtcgt cgttcctgat gcagtactac gccaccaccg
      661 agccgccacg cgagatctac gtgaacctcc ggctgcccga tgccgaagcc ctcgccacgg
      721 cgctcggcga acgcgcctcg cgccgcgtcg gcgtgagtcg tccgtcgcga gggctggcgg
      781 cgcgctgggt ggccctcact gtggagaacg ccacgcaggc gctgcgcatg cgccgcgcac
      841 agagcgaggg cgtggccgaa atgcgcgccg ctctggccgc tgccctcgac ctgcccgcgc
      901 caccggagcg catggagtgc ttcgacatca gccacacggg aggcgagggc accgtcgcat
      961 cctgcgtcgt cttcgggccc gatggcccgc tgaagaagga gtaccggcgc ttcaacatca
     1021 ccggcattgc gcccggtgac gactacgggg cgctgcgtca ggcactcacg cgacgctata
     1081 cgcgcgtgcg cagcggcgag atcccgcctc ctgatgtgct cttcatcgac ggcggtcgcg
     1141 gtcaggtggg cgaggtgcgc gacgtgctcg ccgaactggg ctttgccgac ctgaccctcg
     1201 tgggggtggc caaaggcgcg gaccgccggc cagggcagga gcgcctgttc gtgtacggcg
     1261 aggaggcgcc gcgcgtcctc gatccgacct cgccggcact gcggctgatc cagcggatcc
     1321 gcgacgaagc gcaccgtttc gcgatcaccg ggcaccgcaa gcgccgcgcg cgacgctaca
     1381 acgaatcggt gctcgaggtc gtgccgggac tgggccccgc ccgtcggcgc gcgctgctga
     1441 agcatttcgg cggcctgcag ggcgtgctga aggcgggtgt cgccgacctc gcgcgtgcgc
     1501 ccggcatagg ccccgcgctc gcgcaaactc tatatgatca cctgcaccct ggtgcatgat
     1561 gatctggaac cttcccaaca ccctgacctg gctgcgcatc ttcgcgatcc cgctcgtggt
     1621 tgcgctgtat tacctgccgt tccggtggtc cgacccggcc gcgggcctgc tgttcgcgat
     1681 cgccggcatc accgactcgc tcgatggcta cttcgcccgc aagtacggcc agacctccaa
     1741 gctcggcgcc ttcctcgatc cggtggccga caaactgatc gtcgcggtcg cgctggtcct
     1801 gatcgtcagc aaggacccgc gctggttcgt cgtgatcatc gcgagcgtga tcatcgggcg
     1861 tgagatcgcg atctcggcgc tgcgcgagtg gatggccgag atcggccagc gcaagaaggt
     1921 cgccgtctcg cgcatcggca agcacaagac gatcctgcag gtcaccggcc tgtcgatgat
     1981 gctgttccgc tgggacctgt tcggcctgcc gatctacgac atcgggctcg tgttgaccgt
     2041 gcttgcggcc gtactcacgc tgtggtcgat gtggctctat ctgcgcgcgg cctggccgga
     2101 gatgcgatcg ggttgacgcc cgggccgtaa gtccttgact tggcaactgg caccactaca
     2161 attcccgccc tcaccacgc
//
LOCUS       DAOQVY010000196         1845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_82509_length_1845_cov_2.177095, whole genome shotgun sequence.
ACCESSION   DAOQVY010000196 DAOQVY010000000
VERSION     DAOQVY010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82509_length_1845_cov_2.177095"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PKN91_12255"
     CDS             complement(<1..554)
                     /locus_tag="PKN91_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNS28882.1"
                     /translation="MKILQINASARADGSNSTRLADAITAKLTSARPDAVVEVRDLAR
                     DPHPVLDEPALAALFTPADRRSPEQAARVALDDAVIAQLQSADAVVLGVPMYNFGVPV
                     QLKAWIDAIARAGVTFRYTAEGPQGLLTGKKVYVALARGGLYRDTPADSQVPYLKSVL
                     GFLGMTDIEFVYAEGLAMGEASAA"
     gene            668..1588
                     /locus_tag="PKN91_12260"
     CDS             668..1588
                     /locus_tag="PKN91_12260"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNS28883.1"
                     /translation="MNLDADDLVLFARVAEAGSFSRASERCGLPKATVSRRIGALEAK
                     LGERLMTRTTRQLAITEFGERILEHARRLLEETEAASALAQHRQATPQGTLRVSLPPG
                     FLDELELVPFLLEFAERHPDVRLELDISPRRVDVIAERFDLAVRVAPRLPDDATLVAR
                     RLIELEQGLFASPAYLARAGRPSSPAQLLAGHAALRLVSSDGEPHPWRLARGGERWEG
                     LPDGPIAANSIGLMSTLARHGMGIAGLSARYAASLVAQGLLERVLPEWCLPTMTVWCV
                     TPGRRLLPRRTSVFVAMLEAQLQRPLTAPR"
     gene            1630..>1845
                     /locus_tag="PKN91_12265"
     CDS             1630..>1845
                     /locus_tag="PKN91_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS28884.1"
                     /translation="MPESFDLFVIGGGSGGVRAARIAAGHGAKVALAEEYRYGGTCVI
                     RGCVPKKLLVLASRLADEFERAPAYGWQ"
BASE COUNT          261 a          630 c          673 g          281 t
ORIGIN      
        1 ttcgccgcgg acgcttcgcc catcgccagg ccctcggcgt agacgaactc gatgtcggtc
       61 atgccgagga agccgagcac cgacttcagg tacggcacct gcgagtcggc cggcgtatcg
      121 cggtacagcc cgccgcgcgc cagcgcgacg tagaccttct tgccggtgag cagcccctgc
      181 ggcccttcgg ccgtatagcg gaaggttacc ccggcgcggg cgatcgcatc gatccaggcc
      241 ttcagttgca cgggcacgcc gaagttgtac atgggcacgc cgagcacgac ggcgtcggcc
      301 gattgcagct gcgcgatcac cgcgtcgtcg agcgcgacgc gcgccgcctg ttcggggctg
      361 cgccggtcgg ccggcgtgaa cagcgcggcg agcgccggct cgtcgagcac cgggtgcggg
      421 tcgcgcgcca ggtcgcgcac ctcgacgacg gcgtccgggc gggcggacgt cagcttcgcg
      481 gtgatcgcat cggcgaggcg ggtcgagttc gagccgtcgg ctcgggcgct ggcgttgatc
      541 tggaggatct tcatgagggt ctcctgcgga ggtggatcgg atgaccccac tgtattgatc
      601 tgaaagtcgc aacagaagcc atcgaaacgg atatcatcga tccacaaatg gaacaatctg
      661 ggcgaccatg aatctcgacg ccgacgacct cgtcctgttc gcgcgcgtcg ccgaggcggg
      721 cagcttctcg cgggcatcgg aacgctgcgg attgccgaag gcgaccgtgt cgcggcgcat
      781 cggcgcgctg gaggccaagc tcggcgagcg gctgatgacg cgcaccacgc gccagctcgc
      841 gatcaccgag ttcggcgagc gcatcctcga gcacgcacga cgcctgctgg aggaaaccga
      901 agccgccagt gcgctggcgc agcaccggca ggcgacaccg cagggcacgc tgcgcgtctc
      961 gcttccgccg ggattcctcg acgagctcga gctggtgccc ttcctcctgg aattcgccga
     1021 gcgccatccc gacgtgcgcc tcgagctgga catctcgccg cgccgggtcg acgtgatcgc
     1081 ggagcgcttc gacctcgcgg tacgcgtcgc gccccgattg ccggacgacg ccacgctggt
     1141 cgcccgccgg ctgatcgagc tcgagcaggg tctgttcgcg agcccggcct atctcgcgcg
     1201 cgccggcagg ccgtcgtcgc ccgcgcagtt gctggccggc cacgccgcct tgcggctggt
     1261 ctcgagcgac ggcgagccgc atccgtggcg actcgcccgt ggcggcgagc gatgggaggg
     1321 cttgccggat ggtccgatcg cggccaactc gatcggcctg atgtcgacgc tggcgaggca
     1381 cggaatgggc atcgccggcc tgtccgcgcg ctacgccgct tcgctggtcg cgcagggcct
     1441 gctcgagcgc gtgctgccag agtggtgcct gccgacaatg accgtgtggt gcgtgacgcc
     1501 gggcaggcgg ctgctgccgc ggcgcacgtc ggtgttcgtg gcgatgctcg aggcgcaatt
     1561 gcagcggccg ctgacggccc cgcgctgatc ggcgtcgggg tccggccgcc cggaagagag
     1621 ggagacacca tgcctgaatc attcgacctg ttcgtgatcg gcggcgggtc gggcggcgtt
     1681 cgcgctgccc gcatcgccgc cggacacggc gcgaaggtgg cgctggccga ggagtaccgc
     1741 tacggcggca cctgcgtgat ccggggctgc gtacccaaga agctgctggt gctggcgagt
     1801 cgcctcgccg acgagttcga gcgcgccccg gcgtatggat ggcag
//
LOCUS       DAOQVY010000197         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_1076
            NODE_104407_length_1602_cov_7.723335, whole genome shotgun
            sequence.
ACCESSION   DAOQVY010000197 DAOQVY010000000
VERSION     DAOQVY010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852419
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:15:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,456
            CDSs (total)                      :: 2,424
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 32
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104407_length_1602_cov_7.723335"
                     /isolate="AMR_MDS_1076"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PKN91_12270"
     CDS             complement(<1..389)
                     /locus_tag="PKN91_12270"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNS28885.1"
                     /translation="MDYDSMAAALARSGQYRVLRRFVPAETYQVGPAVDPSRVRAVLV
                     VDVETTGLRKETDRIIDLGFVLAEFDRLTGQVMRVLERYSGFEDPEGPIPAAITQLTG
                     IRDEDVRGQRLDDARVEAAIARADLVVA"
     gene            complement(448..579)
                     /locus_tag="PKN91_12275"
     CDS             complement(448..579)
                     /locus_tag="PKN91_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS28886.1"
                     /translation="MIPSLLHVARGAWCGTLGMRQRLKPIGETRSKGFETVSVAQGM"
     gene            889..>1602
                     /locus_tag="PKN91_12280"
     CDS             889..>1602
                     /locus_tag="PKN91_12280"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNS28887.1"
                     /translation="MRYTTLLVSFLLAGCNAAYIPERTARPVIAPLPSATGDSARFKL
                     VTGKTATGHRDFANAKKILPIVFLQMERDFYCGCRYTGTTVDFASCGYVPRKNLTRAR
                     RIEWEHVVPAWVLGHQRQCWQVGGRAHCVENDPVFQVAEGDLNNLVPAVGEVNGDRSN
                     YAYSAWTRTPTPMYGRCETIVDFKLKKAQPREAVRGRAARITLYMARTYDLRLSAQDK
                     KLMCAWAKTYPVDDWERERN"
BASE COUNT          308 a          559 c          465 g          270 t
ORIGIN      
        1 gcgacaacca gatccgcgcg cgcaatggcg gcctcgacgc gcgcgtcatc gagccgctgt
       61 ccacggacat cctcgtcacg aatgccggtg agttgcgtga tcgccgcggg gatcggtccc
      121 tcgggatcct cgaaaccgct gtagcgctcc agcacccgca tcacctgccc ggtgagacga
      181 tcgaattcgg ccagcacaaa gcccagatcg atgatcctat cggtttcctt cctgaggccg
      241 gtcgtctcca catcgacgac cagcacggcg cgcacccgcg acggatcgac cgccgggccg
      301 acctgatagg tctcggcggg cacaaaccgg cgaagcaccc gatactgccc gctccgcgcc
      361 agggcagcgg ccatgctgtc gtaatccacc ccaatttcct cactgccttg ccgatcgcaa
      421 tccacgaggc tctgccgttg agctcactca catgccttgg gccaccgaca ccgtctcgaa
      481 gcccttgctg cgagtttcac cgatcggctt cagccgctgg cgcatcccga gggtaccgca
      541 ccacgcccct ctggcgacgt gcagcagtga cggaatcatc ccgttagaat acgcgcgctc
      601 ccacgtcggg ctcgatgata aaggtgcaag atcacgcggg caggcgccga gacccgagaa
      661 cgccgtagcc aacgcgaact cgcgcacacc gactgccgga accagtacac caccccatgg
      721 tgcgcatggc cgccacaacg cggccggcgg tgcgcacccc agcgccatac gggccctgca
      781 aaccctcgtg ccaaattatc cgtgacggag ttgcatggtc cgcaccaatc ccatcgcctc
      841 aagatcctca gcccgaatcg cccttgggcc tgcagtcgaa ctttacccat gcgctacacc
      901 acgttgttgg tcagcttctt gctggccggc tgcaatgcag cctacattcc cgaacgtact
      961 gcccgtccag tcatcgctcc gcttccgtcc gcaaccgggg atagcgcgcg cttcaagctt
     1021 gttactggca agacggccac cggtcatcgc gatttcgcca acgccaagaa gatcctgccc
     1081 atcgtatttc tccagatgga gcgggatttc tactgcggct gccggtacac cgggacgacg
     1141 gtcgatttcg ccagctgtgg ttacgtgccg cgcaagaacc tgacccgtgc ccgtcgcatc
     1201 gagtgggaac acgtcgtccc cgcgtgggtg ctggggcatc agcgtcagtg ctggcaggta
     1261 ggcgggcgcg cccattgtgt cgagaacgat ccggtgttcc aggtggccga gggggatctc
     1321 aacaacctcg tcccggcagt gggggaggtc aacggggacc gcagcaatta cgcctacagt
     1381 gcctggaccc gaacgccgac gcccatgtat ggccgctgcg aaaccatcgt ggacttcaag
     1441 ctgaagaaag cgcagccacg cgaggctgtt cgtggtcgcg ccgcgcgcat cacgctctac
     1501 atggcgcgga cctatgacct gcgcctgagt gctcaagaca agaaactcat gtgcgcctgg
     1561 gcgaaaacct acccggtcga cgactgggag cgcgagcgca at
//
